LOCUS       DANKHJ010000001       130071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000001 DANKHJ010000000
VERSION     DANKHJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 130071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 130071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            212..604
                     /locus_tag="PW113_000001"
     CDS             212..604
                     /locus_tag="PW113_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2524346.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PW113_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PW113_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2524347.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PW113_000003"
     CDS             3165..4775
                     /locus_tag="PW113_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2524348.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PW113_000004"
     CDS             5067..5936
                     /locus_tag="PW113_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2524349.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PW113_000005"
     CDS             6101..7372
                     /locus_tag="PW113_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2524350.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PW113_000006"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PW113_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2524351.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PW113_000007"
     CDS             8763..9026
                     /locus_tag="PW113_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2524352.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PW113_000008"
     CDS             9471..9920
                     /locus_tag="PW113_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2524353.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PW113_000009"
     CDS             9977..11395
                     /locus_tag="PW113_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2524354.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PW113_000010"
     CDS             11395..12255
                     /locus_tag="PW113_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2524355.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PW113_000011"
     CDS             12429..13202
                     /locus_tag="PW113_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2524356.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PW113_000012"
     CDS             13349..13714
                     /locus_tag="PW113_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2524357.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PW113_000013"
     CDS             complement(13862..15070)
                     /locus_tag="PW113_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2524358.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PW113_000014"
     CDS             complement(15089..15367)
                     /locus_tag="PW113_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524359.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PW113_000015"
     CDS             15521..16216
                     /locus_tag="PW113_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2524360.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PW113_000016"
     CDS             complement(16658..17455)
                     /locus_tag="PW113_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2524361.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PW113_000017"
     CDS             complement(17452..18636)
                     /locus_tag="PW113_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2524362.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PW113_000018"
     CDS             complement(18661..19908)
                     /locus_tag="PW113_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2524363.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PW113_000019"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PW113_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2524364.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PW113_000020"
     CDS             21888..22202
                     /locus_tag="PW113_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2524365.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PW113_000021"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PW113_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2524366.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PW113_000022"
     CDS             22887..23531
                     /locus_tag="PW113_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2524367.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PW113_000023"
     CDS             23546..23875
                     /locus_tag="PW113_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2524368.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PW113_000024"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PW113_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2524369.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PW113_000025"
     CDS             24863..25687
                     /locus_tag="PW113_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL2524370.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PW113_000026"
     CDS             25680..26027
                     /locus_tag="PW113_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2524371.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PW113_000027"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PW113_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2524372.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PW113_000028"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PW113_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2524373.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PW113_000029"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PW113_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2524374.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PW113_000030"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PW113_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2524375.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PW113_000031"
     CDS             31657..32451
                     /locus_tag="PW113_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524376.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PW113_000032"
     CDS             32441..34483
                     /locus_tag="PW113_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524377.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="PW113_000033"
     CDS             34764..34913
                     /locus_tag="PW113_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2524378.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="PW113_000034"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="PW113_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2524379.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="PW113_000035"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="PW113_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2524380.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="PW113_000036"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="PW113_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2524381.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="PW113_000037"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="PW113_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2524382.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="PW113_000038"
     CDS             39330..40412
                     /locus_tag="PW113_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2524383.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="PW113_000039"
     CDS             40412..41215
                     /locus_tag="PW113_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2524384.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="PW113_000040"
     CDS             41285..42025
                     /locus_tag="PW113_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2524385.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="PW113_000041"
     CDS             42036..43040
                     /locus_tag="PW113_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2524386.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="PW113_000042"
     CDS             complement(43085..44929)
                     /locus_tag="PW113_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524387.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="PW113_000043"
     CDS             45140..45658
                     /locus_tag="PW113_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2524388.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="PW113_000044"
     CDS             45786..46469
                     /locus_tag="PW113_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2524389.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="PW113_000045"
     tRNA            46778..46851
                     /locus_tag="PW113_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="PW113_000046"
     CDS             47010..47369
                     /locus_tag="PW113_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2524390.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="PW113_000047"
     CDS             47492..49123
                     /locus_tag="PW113_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2524391.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="PW113_000048"
     CDS             complement(49185..49826)
                     /locus_tag="PW113_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2524392.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="PW113_000049"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="PW113_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2524393.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="PW113_000050"
     CDS             50443..50787
                     /locus_tag="PW113_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2524394.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="PW113_000051"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="PW113_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2524395.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="PW113_000052"
     CDS             51527..54334
                     /locus_tag="PW113_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2524396.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="PW113_000053"
     CDS             54525..55016
                     /locus_tag="PW113_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2524397.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="PW113_000054"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="PW113_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2524398.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="PW113_000055"
     CDS             56141..56944
                     /locus_tag="PW113_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2524399.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="PW113_000056"
     CDS             56965..57879
                     /locus_tag="PW113_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2524400.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="PW113_000057"
     CDS             57962..58336
                     /locus_tag="PW113_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2524401.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="PW113_000058"
     CDS             58475..58720
                     /locus_tag="PW113_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2524402.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="PW113_000059"
     CDS             58892..59956
                     /locus_tag="PW113_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2524403.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="PW113_000060"
     CDS             60202..60627
                     /locus_tag="PW113_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2524404.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="PW113_000061"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="PW113_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2524405.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="PW113_000062"
     CDS             61166..62020
                     /locus_tag="PW113_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2524406.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="PW113_000063"
     CDS             62021..63358
                     /locus_tag="PW113_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2524407.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="PW113_000064"
     CDS             63363..63587
                     /locus_tag="PW113_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2524408.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="PW113_000065"
     CDS             63589..64479
                     /locus_tag="PW113_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2524409.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="PW113_000066"
     CDS             64488..65306
                     /locus_tag="PW113_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2524410.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="PW113_000067"
     CDS             65413..65862
                     /locus_tag="PW113_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2524411.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="PW113_000068"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="PW113_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2524412.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="PW113_000069"
     CDS             complement(67617..67733)
                     /locus_tag="PW113_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2524413.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="PW113_000070"
     CDS             complement(67806..68762)
                     /locus_tag="PW113_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2524414.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="PW113_000071"
     CDS             68933..69301
                     /locus_tag="PW113_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2524415.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="PW113_000072"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="PW113_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2524416.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="PW113_000073"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="PW113_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2524417.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="PW113_000074"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="PW113_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2524418.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="PW113_000075"
     CDS             71289..73481
                     /locus_tag="PW113_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2524419.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /locus_tag="PW113_000076"
     CDS             73507..74469
                     /locus_tag="PW113_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2524420.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /locus_tag="PW113_000077"
     CDS             74483..75844
                     /locus_tag="PW113_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2524421.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="PW113_000078"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="PW113_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2524422.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="PW113_000079"
     CDS             76973..78193
                     /locus_tag="PW113_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2524423.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="PW113_000080"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="PW113_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2524424.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="PW113_000081"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="PW113_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2524425.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="PW113_000082"
     CDS             80955..81632
                     /locus_tag="PW113_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2524426.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="PW113_000083"
     CDS             81646..82245
                     /locus_tag="PW113_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2524427.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82260..82547
                     /locus_tag="PW113_000084"
     CDS             82260..82547
                     /locus_tag="PW113_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2524428.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="PW113_000085"
     CDS             82596..83378
                     /locus_tag="PW113_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2524429.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="PW113_000086"
     CDS             83399..84106
                     /locus_tag="PW113_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2524430.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="PW113_000087"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="PW113_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2524431.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /locus_tag="PW113_000088"
     CDS             complement(87215..88741)
                     /locus_tag="PW113_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2524432.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="PW113_000089"
     CDS             complement(88756..89403)
                     /locus_tag="PW113_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2524433.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="PW113_000090"
     CDS             complement(89587..89916)
                     /locus_tag="PW113_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2524434.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="PW113_000091"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="PW113_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2524435.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="PW113_000092"
     CDS             90837..91850
                     /locus_tag="PW113_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2524436.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="PW113_000093"
     CDS             91850..93127
                     /locus_tag="PW113_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2524437.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="PW113_000094"
     CDS             93190..95892
                     /locus_tag="PW113_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2524438.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="PW113_000095"
     CDS             96044..96361
                     /locus_tag="PW113_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2524439.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="PW113_000096"
     CDS             96391..96711
                     /locus_tag="PW113_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2524440.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="PW113_000097"
     CDS             96819..98279
                     /locus_tag="PW113_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2524441.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="PW113_000098"
     CDS             98347..98568
                     /locus_tag="PW113_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524442.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="PW113_000099"
     CDS             98599..98781
                     /locus_tag="PW113_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2524443.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="PW113_000100"
     CDS             98781..99194
                     /locus_tag="PW113_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2524444.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="PW113_000101"
     CDS             99317..100498
                     /locus_tag="PW113_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2524445.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100569..100733)
                     /locus_tag="PW113_000102"
     CDS             complement(100569..100733)
                     /locus_tag="PW113_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524446.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100887..100960
                     /locus_tag="PW113_000103"
     tRNA            100887..100960
                     /locus_tag="PW113_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100921..100923,aa:Arg,seq:cct)
     gene            complement(101029..102168)
                     /locus_tag="PW113_000104"
     CDS             complement(101029..102168)
                     /locus_tag="PW113_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2524447.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102467..103102)
                     /locus_tag="PW113_000105"
     CDS             complement(102467..103102)
                     /locus_tag="PW113_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524448.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103215..103850)
                     /locus_tag="PW113_000106"
     CDS             complement(103215..103850)
                     /locus_tag="PW113_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2524449.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103884..104345)
                     /locus_tag="PW113_000107"
     CDS             complement(103884..104345)
                     /locus_tag="PW113_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2524450.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104475..104906)
                     /locus_tag="PW113_000108"
     CDS             complement(104475..104906)
                     /locus_tag="PW113_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2524451.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104924..105244)
                     /locus_tag="PW113_000109"
     CDS             complement(104924..105244)
                     /locus_tag="PW113_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2524452.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105543..106319
                     /locus_tag="PW113_000110"
     CDS             105543..106319
                     /locus_tag="PW113_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2524453.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106334..106537
                     /locus_tag="PW113_000111"
     CDS             106334..106537
                     /locus_tag="PW113_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2524454.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106553..106891
                     /locus_tag="PW113_000112"
     CDS             106553..106891
                     /locus_tag="PW113_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2524455.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106878..107057
                     /locus_tag="PW113_000113"
     CDS             106878..107057
                     /locus_tag="PW113_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524456.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107100..107570)
                     /locus_tag="PW113_000114"
     CDS             complement(107100..107570)
                     /locus_tag="PW113_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524457.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107657..108355
                     /locus_tag="PW113_000115"
     CDS             107657..108355
                     /locus_tag="PW113_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2524458.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108533..108874
                     /locus_tag="PW113_000116"
     CDS             108533..108874
                     /locus_tag="PW113_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524459.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108867..109538
                     /locus_tag="PW113_000117"
     CDS             108867..109538
                     /locus_tag="PW113_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2524460.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109544..110236
                     /locus_tag="PW113_000118"
     CDS             109544..110236
                     /locus_tag="PW113_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524461.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            110233..110982
                     /locus_tag="PW113_000119"
     CDS             110233..110982
                     /locus_tag="PW113_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2524462.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110994..111263
                     /locus_tag="PW113_000120"
     CDS             110994..111263
                     /locus_tag="PW113_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524463.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            111269..111433
                     /locus_tag="PW113_000121"
     CDS             111269..111433
                     /locus_tag="PW113_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524464.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111426..111725
                     /locus_tag="PW113_000122"
     CDS             111426..111725
                     /locus_tag="PW113_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524465.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            111725..112027
                     /locus_tag="PW113_000123"
     CDS             111725..112027
                     /locus_tag="PW113_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2524466.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            112067..112432
                     /locus_tag="PW113_000124"
     CDS             112067..112432
                     /locus_tag="PW113_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524467.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            112922..113062
                     /locus_tag="PW113_000125"
     CDS             112922..113062
                     /locus_tag="PW113_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524468.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            113059..113241
                     /locus_tag="PW113_000126"
     CDS             113059..113241
                     /locus_tag="PW113_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524469.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            113254..113448
                     /locus_tag="PW113_000127"
     CDS             113254..113448
                     /locus_tag="PW113_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524470.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(113481..113693)
                     /locus_tag="PW113_000128"
     CDS             complement(113481..113693)
                     /locus_tag="PW113_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524471.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            113818..114021
                     /locus_tag="PW113_000129"
     CDS             113818..114021
                     /locus_tag="PW113_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524472.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            114018..114290
                     /locus_tag="PW113_000130"
     CDS             114018..114290
                     /locus_tag="PW113_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL2524473.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            114291..114476
                     /locus_tag="PW113_000131"
     CDS             114291..114476
                     /locus_tag="PW113_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524474.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            114463..114810
                     /locus_tag="PW113_000132"
     CDS             114463..114810
                     /locus_tag="PW113_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524475.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            114770..115246
                     /locus_tag="PW113_000133"
     CDS             114770..115246
                     /locus_tag="PW113_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2524476.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(115377..116105)
                     /locus_tag="PW113_000134"
     CDS             complement(115377..116105)
                     /locus_tag="PW113_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL2524477.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            116153..116431
                     /locus_tag="PW113_000135"
     CDS             116153..116431
                     /locus_tag="PW113_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524478.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            116433..116819
                     /locus_tag="PW113_000136"
     CDS             116433..116819
                     /locus_tag="PW113_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524479.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            116816..117196
                     /locus_tag="PW113_000137"
     CDS             116816..117196
                     /locus_tag="PW113_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2524480.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            117308..117760
                     /locus_tag="PW113_000138"
     CDS             117308..117760
                     /locus_tag="PW113_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2524481.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            117757..119484
                     /locus_tag="PW113_000139"
     CDS             117757..119484
                     /locus_tag="PW113_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2524482.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            119548..120735
                     /locus_tag="PW113_000140"
     CDS             119548..120735
                     /locus_tag="PW113_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2524483.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            120707..121276
                     /locus_tag="PW113_000141"
     CDS             120707..121276
                     /locus_tag="PW113_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL2524484.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            121289..122653
                     /locus_tag="PW113_000142"
     CDS             121289..122653
                     /locus_tag="PW113_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2524485.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            122655..122933
                     /locus_tag="PW113_000143"
     CDS             122655..122933
                     /locus_tag="PW113_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2524486.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            122914..123240
                     /locus_tag="PW113_000144"
     CDS             122914..123240
                     /locus_tag="PW113_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2524487.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            123230..123568
                     /locus_tag="PW113_000145"
     CDS             123230..123568
                     /locus_tag="PW113_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524488.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            123558..123923
                     /locus_tag="PW113_000146"
     CDS             123558..123923
                     /locus_tag="PW113_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2524489.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            123930..124556
                     /locus_tag="PW113_000147"
     CDS             123930..124556
                     /locus_tag="PW113_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2524490.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            124556..125020
                     /locus_tag="PW113_000148"
     CDS             124556..125020
                     /locus_tag="PW113_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524491.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            125215..127524
                     /locus_tag="PW113_000149"
     CDS             125215..127524
                     /locus_tag="PW113_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2524492.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            127536..128240
                     /locus_tag="PW113_000150"
     CDS             127536..128240
                     /locus_tag="PW113_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2524493.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            128237..>130071
                     /locus_tag="PW113_000151"
     CDS             128237..>130071
                     /locus_tag="PW113_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2524494.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        43155 a        21837 c        27227 g        37852 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagttt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
   100981 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
   101041 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
   101101 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
   101161 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
   101221 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
   101281 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
   101341 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
   101401 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
   101461 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
   101521 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
   101581 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
   101641 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
   101701 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
   101761 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
   101821 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
   101881 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
   101941 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
   102001 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
   102061 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
   102121 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
   102181 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
   102241 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
   102301 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
   102361 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
   102421 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
   102481 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
   102541 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
   102601 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
   102661 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
   102721 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
   102781 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
   102841 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
   102901 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
   102961 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
   103021 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
   103081 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
   103141 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
   103201 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
   103261 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
   103321 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
   103381 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
   103441 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
   103501 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
   103561 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
   103621 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
   103681 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
   103741 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
   103801 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
   103861 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
   103921 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
   103981 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
   104041 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
   104101 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
   104161 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
   104221 gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct aataattgaa
   104281 atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa aatctctttt
   104341 tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc atatatgagg
   104401 aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
   104461 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
   104521 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
   104581 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
   104641 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
   104701 tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc atgagataaa
   104761 tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat tctgggtttt
   104821 ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt gtattcgctc
   104881 aacatttctt tcaatttcaa atacatacaa accatcactc caactattca ttttcctcta
   104941 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
   105001 aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct ctttcttctc
   105061 tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt aattcatctg
   105121 aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca aaattaattt
   105181 ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt tcataggtat
   105241 tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa tacgcagaaa
   105301 taatttgact aattctgagt aatgagctat actatatctt gtaaacagat ttaacaacta
   105361 aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa gctttaaaat
   105421 caatgttttt atgtcttatt taattatgct cttagtatag agtattactc agatactgtc
   105481 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
   105541 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
   105601 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
   105661 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
   105721 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
   105781 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
   105841 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
   105901 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
   105961 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
   106021 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
   106081 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
   106141 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
   106201 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
   106261 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
   106321 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
   106381 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
   106441 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
   106501 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
   106561 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
   106621 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
   106681 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
   106741 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
   106801 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
   106861 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
   106921 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
   106981 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
   107041 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
   107101 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
   107161 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
   107221 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
   107281 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
   107341 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
   107401 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
   107461 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
   107521 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
   107581 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
   107641 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
   107701 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
   107761 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
   107821 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
   107881 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
   107941 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
   108001 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
   108061 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
   108121 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
   108181 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
   108241 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
   108301 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
   108361 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
   108421 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
   108481 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
   108541 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
   108601 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
   108661 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
   108721 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
   108781 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
   108841 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
   108901 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
   108961 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
   109021 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
   109081 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
   109141 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
   109201 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
   109261 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
   109321 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
   109381 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
   109441 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
   109501 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
   109561 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
   109621 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
   109681 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
   109741 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
   109801 cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa
   109861 tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca
   109921 tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta
   109981 caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca
   110041 ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt
   110101 tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa
   110161 aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat
   110221 tggaggggaa aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg
   110281 tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt
   110341 aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat
   110401 aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa
   110461 atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac
   110521 cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct
   110581 attaataata catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa
   110641 tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct tttgtcagat
   110701 gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg aatcgaacga
   110761 ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc aactattcga
   110821 agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca
   110881 ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct caagaaaaaa
   110941 gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga aaaatgaaga
   111001 tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag gaattagacg
   111061 tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc
   111121 gttcgagttg gagtaaactc agatactatt tcgcactcta tatcgatgga acgcaattaa
   111181 gaaatttagc atataccaaa ttactattca ttgattgtgt taaaggcttg caaaaacatc
   111241 ttaatgaact tgagcaggtg taaacaagat ggacctaaag acatttacag cacagatcga
   111301 attaatgcat caagaagctt taagacaaag tgtgtcgtac gaagacaagt ggctcaacac
   111361 atttcatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
   111421 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
   111481 ggcaggaaga taaaaaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
   111541 aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg gcatggtgat cgaattctag
   111601 taacgggtac cgctaaacag ttatctgaaa ttactggtct gagcaaaaat attatttggg
   111661 atagagctag gagcttatgg attgattcaa aaggaagaca gtttaggtat gtggaggaga
   111721 aataatggac gaactaatcg cgaaagtaga gcagtgggca aaagataagg gattggatca
   111781 agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa atcggggaag ttgccgcttc
   111841 tctagctaga aaagatgaac atggtttaag agacggaatt ggcgatgtag tagtaacctt
   111901 gatcatcttg gcaatgcaaa atgatatgga tttatacgag tgcttggatt ttgcttatga
   111961 cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt ttcgttaagt ccagtgacct
   112021 gaaataattc cattaatgga agaaataact gaataggagg atagacatga ttgatgcaaa
   112081 acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa gcatatgttc taccatttct
   112141 taatcaattt gaagttttgg aatgctctgc cagcggagaa gaattggagt atgttgtaat
   112201 tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt ctttgcacta ttaactgttg
   112261 ggatatgata gctccaggat ttttatgtcc agctatggga gaatttttgg aatattgtcg
   112321 tcttgaagat gcaggcactt tggatttagc ttatctggta tacaactacc taaacatcaa
   112381 cacagatcat ttgtggtttg gcactgccga aagaaaatgg gtagtccgct aaccaccaat
   112441 atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg ccattgctgt aaggttcttt
   112501 acgcgactca aggtcacaga aatataaatg caaatctctt ttgaagtgat ccaaagctct
   112561 cggaaaatgg aagttttttg tttcaaagaa taaatgagat ttgcgtcaag aacctgagag
   112621 ttatttccaa aaaagcttcc aaagaaccga atgagctaag gacatcatgt gtaaacacat
   112681 aatatagctc tcctttcacg gctaatcata acatgaaaaa tattcgatgt cagtaggaaa
   112741 aaaggtgaat tatgaaaaga atattaaatt atcccggcag taacagattc tgcaattaaa
   112801 ctccgaagct gatgtatcag tatcaactgt agaatattat gagcgtggag aaagtcaagt
   112861 agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc tgtttagatg aaggcgatag
   112921 tatgaatgaa attgctgcag accatgaatt atcagaaact gctttaattg atcaaatcga
   112981 attagctaga caatactttg ttccatatgc agatacctgg tccaagcata agaatgacat
   113041 catttttcca gaggaacaat gagagcaact gatccagtaa ttatccttga ggaagccaaa
   113101 tttatttgga ctcacgaaga gatagagcaa gcacgcttgc tcttttctca aggagttaag
   113161 ccgagcaaag tagctgaaat aatgggtcaa aagattcttg atgtcggatt gcttttgctc
   113221 catctagcag aaaaaatttg atttgaggtg aaaatgatga ttcaacttgc aggcatccaa
   113281 acagggaaaa tttatttttc tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa
   113341 acttacacga acaataagaa gctaaggaaa aaatatcctg atttgttgct aaaggatgat
   113401 cagattatgc cggaaccgat gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc
   113461 agccgtgtgg gctggagttg ctatttcttt ttaataatga gtttttctaa atttaattca
   113521 ttctcacgag tttgcgtcca ataatcagaa taatatttta attgtttaag agtaggaatg
   113581 tcgtcttcgg taaaattaga aaataattct ggagaattat ctttttttaa ttgttctaat
   113641 cctgattctt taaaacgttg ttcaacgagt tttactaaat tgttgatatt catgataaat
   113701 caccttttta aatttaattt cgatttgatt aatcgataca agtattatat caaataaaaa
   113761 agctgattta gggggtctta caaaaacact aaaatttttt caaaaagagg aggaacggtg
   113821 gtgaaagtca gagaacaatt caagatagtg agcgacgaag gtaatgttta caaaataaag
   113881 atcgttaata ttaatgaata tcgagaagca gatcacaaat atttggttga cttatacatg
   113941 aacgataaat atttgggaga aggattttgt ggagattcac tttttgatga tgagcgaatt
   114001 ttgaaggagg aaacagaatg atccacgaac taaaaatact accagaatat tttgaagcag
   114061 tgacgagcgg acgcaaacaa ttcgagatcc gcaagaatga tcgtgacttc aaagttggtg
   114121 atcagttaat actacgtgaa tataaaaaat acattaaaca tgaattacct ggactaacga
   114181 gaccaacatt tagttacact ggtgactcat acaaagcaga gatcacttat attactgatt
   114241 acatgcagaa gtccggatat gtagtgttgg ggattaaagg ggtgaattaa atgaaagtat
   114301 acatcttagc tatcacagag ggcacatgga tgttcccagt cggttccgga aaaatctata
   114361 aatcaaaaac agctgcttat aaagcatttg aaaaatacaa aaaagaaaac ggtggcggca
   114421 ctaacgcaaa gattctagtg gctgataact ggcatgaaga aggtgaacgt aattgattta
   114481 cctgttcatt ttcgtggtac taactatttt tatgctagta atcttattca aaaaaagatg
   114541 agtaaagacg aacgaaaaat tctggccaac ttttatttag tattaaaaat cgaacaaaat
   114601 gtgttttcgg aaaggagaat tgtatgggaa aaacaaaatc aaagattaag aagaaaaaac
   114661 gacgtttgga acaaaaggca atccagaatg gaacggctaa gaaaaaataa aaaagctacc
   114721 cctttcgaga tagcagcgca atactattct accataaggg gtggcgtttg tgagatttca
   114781 ttggttaaaa gattaccaag aactagatga gcagattctt tacttaaagt ggaatcttaa
   114841 taaaagtaag cttgaattga atcgatgggt ctgtggtgat ttagcaaacg tccgcatcga
   114901 aaagaattcg agaacttctt ctttagaaga gaatatccaa aagatagaaa atgaattgga
   114961 attgttgatc gagcaaaaag aagaaatgtt attgctgata gatagctttt ctggtatcga
   115021 taatcaaatt gttaagatga aatatgttga tcaaatgagc ttagaagata ttgctgaatc
   115081 tgttgggtat agctcatcgt atgtcagaca gcgacatgca gaaattagaa agacattgaa
   115141 ctttttagat gagtatgaac acagacaagc tgaccgattg aaaaaagaaa acgaaattga
   115201 tttttacaat agcgagaagt acaaagaaca actctcttta ttctaaaatt acactgttca
   115261 ctaaatgttc gtaaacattg ttagcagcat gatattctat tagtgtcaaa aaaatatgaa
   115321 agagccaaga tatcccaacc gttttattaa ttggtatctg tggctctttt attcttttaa
   115381 ttagtagttg gcataaagtt atttccattt ataaattgat cagctaaatc agcatcataa
   115441 tcagccgcga taaattgcgc tgctgattta ataaatgcat ccatatcatg aacatcttta
   115501 ttagtatgtc tacgaacata gtgcgtttca tcattaccaa tccaagtagc agctttggct
   115561 aatctttgga gtttaggaaa atcattcaaa taattttcaa ttgttttacc taataacatt
   115621 ttttttattt tatcttcatc ctctgaattt ttagatattg cataatcttt aattagaaat
   115681 tctagagctt ttctataacc aactccagca atttgttcca attgctcaga tttagcttgt
   115741 gtggcttgag tataaatatc aacaaatgta ggagaaactt tttcaatatt ttctggtaaa
   115801 tctattttaa tcggcggacg gtaataatct ttaattaaaa tattttcaga atatccttca
   115861 tcttcattaa acgtatttat aaattgtaaa ctataaaatt tgttgcactc atcatttata
   115921 cactgtacta atacgccaaa gattctatca ttatcgaacg cactatatga tgaaacccca
   115981 aaatacacat acggagtcat tatatttccg caatgcggac aactatttgg caaataaatt
   116041 gtctctgtat tttgacgatc acgatactta atattaactt cttgaagcat taaaacacct
   116101 cgcatctttt ttataaatca taccacaaaa tatctgatag gagagtaaac aaatgaatga
   116161 ttttcatgaa gcggtactta gtattgaagt agaatcaagt ttggctaaag tttataagaa
   116221 agcaattgaa gcagagaata gtccgtacag agaaaattgg aatggtaatc atgcacatgt
   116281 gcaagtggag aatgatgatt acagaactgg aatgaatacg ttagttattt ctttattgtc
   116341 acacactcta cccaatctgc aggaaacgat tgaatggtat gagcgaatgg gcgcaaaagt
   116401 aatccgtaca aattacaaag gagagaatta aaatggcaat gatcaaaatt agaacatcca
   116461 ttagtggaac agagtattgg gattcagaaa agaaaaggac agtagttgtt ccgaaagatc
   116521 aagaaccaaa ttttgaagta aaagaagaaa aaggaatttt gagtgatggc aaatcatttg
   116581 ttgctgtcaa tggcgaactc atagctcata gcagtgaaat tcttgatagc gatggaaaca
   116641 ctgctgctaa ttttgatgga gacgaagcta ctaatgatca gtccactgaa gatacggatg
   116701 aactggacaa tatgactgca aaagaattgc gtgcatatgc taagaaacat ggtattgata
   116761 ttcctggtgc tatccgtgca aaaggcgata tcataaatct tattcgtgaa gtagaatgaa
   116821 atactgtcag tttgacggat gcacgaacaa aatagcaaag ggtatctact gtactgaaca
   116881 caagagatca agcagatcac gcaagaagaa gcagcaagca aagtctgttt atcatcatga
   116941 gaacaaacca ttctatcgaa cgcaagcatg gaaagatatg cgtcaattta tttatgaaag
   117001 agaaggtggt cattgtcagc ggtgtggtca gttcatattt ggaaagagag cacacgtcca
   117061 tcacattgta ccaatcaaag acaacgaact gcttaagctt gatccaaaca atctcatgct
   117121 attatgttca aaatgtcatc caattgttga aaacgaaacg gaagacaaaa aagtttttcc
   117181 ttcgtatttc aattgaagcc cccctattca ttttcaaaat tttttcgcgt ggggagatag
   117241 ggtagcgggg agtcacgcgc atcgttaggt caaatttttc aaaaaacaaa ggggggtgta
   117301 tacaaaaatg acgactaaag cgcaacgtaa agcgattatt gatgaaaaag taagtgctga
   117361 aaaagctcgt atcttagaaa taatgaattt gtctgatttg tacaccatca ctcttgatcc
   117421 attaatcgaa tcatacttgg atatttttga aatttaccaa cacaaatatt tattgtggaa
   117481 ggaaaaagga tttccggaga cgcaaaaatt cacgaataaa tcaggagcta ctaatcaatc
   117541 aaaacatcca ttggcacaac aagtagaaac ttgggcagat aagaagatga aagctctaga
   117601 tttattagga ttaacaaaca aagcaaaaac tggtagacta attgctggag gatcgaccgc
   117661 aagaaaagat gaagaaatta cacgcccaga agtaaagcca gtagatgaac tagcagcgca
   117721 cagaaataaa tggcgtaaga aggcaggtgc tgaaaaatga ttgaacctgg tgtaaattat
   117781 gctgatttat ttgcaaaaga agtaagaaag aaacctaaga agtatcctaa gaccgttcgt
   117841 ttagcaatag atcgctggta tcagtggaaa aaaagaaaag atatttggtt tgatgtagat
   117901 cgcgcgaatg aaatgatgga ttgggtagaa tcatttattg ttcacacaaa aggagaaatg
   117961 gtgggaaaac cgtttctttt agaaccctgg gaaaaattta tttattcttg gatttatggt
   118021 tgggttaaag aaaatgaaaa aggccaaatt gtacgcgtta ctcgtgaggc ttatgtccag
   118081 attccaaaaa agaatggtaa aacattgatt gctgtcggtt cattaggata tgcaatgtac
   118141 ggagaagggg cgttatcagt tgattgctat gcttgcgcat ctgattttgc tcaagcgcaa
   118201 tatgctgcca aaccttttgc agttacaatc ttaaacaatc caatcctact tgaaggaact
   118261 aaaatattta gggggccaaa gggaactgtt tctagtatta cttatgacta tattcatgaa
   118321 gatatggctt actcaaataa atttattgtt caaaccaaga atattgataa tatcgaggga
   118381 tccaatccat attttgtatt gaacgatgaa ttgcataaac aggagaaaat ggaacagtat
   118441 gacaatttta agtctgctca gatatcttta ccacagccat tgatgtttaa tatctcaact
   118501 gctgggaaag gttcgtcgtc tgttggaatg cgtgtatatc gcgaagcaaa agaagtgttg
   118561 aaacgtgatg ataatgattc aaactttgtt ctaatctatg agccaaataa aaattatgat
   118621 tggacggata gaaaagtctg ggaaatgtgc aatcctaact ggggaatatc agtcgattta
   118681 tctgctttag aatcggcatt taaaacagcg caacggtcag ctcattccaa agctgaattt
   118741 ctaacgaaac acttggatgt atttgtgaac ggcgcggata atttctttga acaggatcaa
   118801 gtagagccat gtttagttac cacacaagaa cttggtgatt taagtggcga accatgttat
   118861 atcggtttag atttatcacg tacacgagat ttgacctgtg tatctttaaa cttcccaaca
   118921 tgggatgaag atggtaaagc ggttcttaaa gtaaagcaac tttattttat tccgaatgaa
   118981 gatttagagt ttcgagaaaa agaagataat gttccttaca gtgatttggc tgaacaaggt
   119041 ttcgttgaat tttgcgacgg aaaaatgatt gatcaagatc agattttgca gtacatagag
   119101 gactgcatga atttatatga tattcagcaa gtgaattatg atccagcgat gagtgacaaa
   119161 cttgttgaga aattagaaaa cttaggattg gaatgcgttg aagttgctca atatcctaaa
   119221 gtattgaatg ctccttttga cgatgtcgaa cggttgtttt atgagaaacg aattcaattt
   119281 gataatccat tattccttta ttgcactttg aacgttgtag caattactaa catcaatgga
   119341 caaaaagcgc caagtaaacg tcaatcaaag aaaaagattg atgggttcgt ggcgtttttg
   119401 tgtggtcata aggaaacgat gaatcaaatg acagatattg attcagatga gttagatgat
   119461 tacctaagtt ccatctatag ataaatagaa aggcggtgaa aaaaattgaa attacgtgat
   119521 cggttatcga atgcagtcta ttcttttatg gaaaaacgtg gatatatcga ggatattttt
   119581 ggacactaca cacgttatgg tcaaagatat gtgacggatt catctatcat ggaatcttct
   119641 gatgtttatg aattagttca agacatatct aatcaggtgg cattggccac accaattgtt
   119701 attggtcccg acggcaacga agtcaaagat catcacttac taaatatttt gaagagtccc
   119761 aatgattatt tgacaggatt cgaatttacc aaattggaaa caaatacttt attgatcaat
   119821 ggtgagacat tcccattaac ggatagggat cagcttcatt tggcgtacgg tgtaacaact
   119881 aaaatcaatg aacgacttca agaagaattt gaaatgaatg gtcaaaaaat acctggtcaa
   119941 atgattcgac acattaagaa catcggaact gattcattaa aaggtgctgg aataattgat
   120001 cttgcaagaa acactctaga aggcgttctg agcgctgaaa aagttttgac ggacaaatat
   120061 actaaaggcg gtttacttgc gttcatgctt aaactagacg cccacatcaa tccaaataat
   120121 agcgcccaaa caaaaattgt caaagctata ttggatcaac tggaaggaac gcaaaatgag
   120181 agtgatcatt ctgttaagat gattcctttg ggaaaaggat attccatcga gacattaaaa
   120241 agtcctgttg atgacgcggc aattttgaat tatttaggtg tttacaaaaa agacttagga
   120301 aaatttctag gaatcaatgt tgatacgtat caatcgctga tgaagacaga tattgaaaaa
   120361 gcgatgatgt atctgcacaa caaagcaatc aaaccaatat tgaaaaacaa gagcgaacat
   120421 tacaccgctc ttttttttat gcctaattct ggctatagag tggaatggaa aattaatatt
   120481 ttggattttg ttccttactc aacaaaaaca aatattggct acaacatcgt tcgtaccggg
   120541 attacaagtc cagataacgt ggcagaaatg cttggttttc ctaaacagaa tactccagaa
   120601 acacaagcta tctatatttc aaatgactta tctaggattg gccagaaaaa tgcaacagat
   120661 gattccttac caacgaatga tcaagacttg aaaggaggtg atggaaatga agaagaagga
   120721 aattcgcacg attgacatca ccaatctttc aacacgttcc gatgaagaaa ctcatacgag
   120781 gaccattagc ggctacgctg ctgtattcaa cagtcaaaca cttttatggg atgacttaag
   120841 cgaagtgatt gttcctggtg cattctcaaa ggcaattagt aattcggatg ttcgttgttt
   120901 gttcaatcac gattggtcca atgtgctagg acgtactaaa agcggaaccc ttcgccttga
   120961 agaagatgaa cgtggtttga aattcgaagt tgatttaccg gacacaacgg tagcaaggga
   121021 cttagttaaa tctatggaac gaggagacat caaccaatgt agttttggtt ttgtgccgac
   121081 tgaagaaacg tgggactaca attctgaacc aatgcttcga acaatccatg aagtggaatt
   121141 atacgaggtt tctattgtgc ctttgccagc atacgaagat acagaagctg cattaagaag
   121201 tcgtgatgaa ttagaaaaaa acgtcgaaga aagaaaaaaa ttaatcaaaa aaattaatca
   121261 agcgctagaa gcgtaggagg aaaaatacat ggataaagaa ttattgaaaa aaatgaaggc
   121321 acgtcgtgag caacgattga ctgaattacg tgaaaaagtt gaatcaggag aattacgcga
   121381 agcagattta gaagccgtga aagaagaaat tgacagtgtt attgatgaat tgaacggaat
   121441 taaagacgaa ttaggcgcag attctggaac tgatgaaaca gacgacaata cagatgatca
   121501 atcgaatagt acggattcag acgaaagccg ttctggtgaa gacaacgatc aagaagaaga
   121561 ttcagacagt gaagatagtt cagaaaatcg ttctggaatg attactcaac agcaacgaga
   121621 tggattactt ggatcaatta agaacggatt ggaggcacgt gcaaaaatga ccaagaaaca
   121681 aaaagatcaa caactacgaa aagcatttgc taattttgta gctggaaata tttctgaagc
   121741 agaagctcga gctttaggga ttgaagctgg caacggttca gttactgtcc cagaagtaat
   121801 tgcatctgaa gttattactt atgctcaaga agaaaattta cttcgtaaat acggaacagt
   121861 ggtgcgaaca tcaggagatg tcaaatatcc aattcttgtg aagaaagcag atgcgaatgt
   121921 aaacaagaaa gagcgttcaa ctgatattgc tgaaacagct attcagtttg atgaaatttt
   121981 gcttaatcct gccgaattcg atgctttggc aacagtaact aaaaaattat taaaaatgtc
   122041 tggtgttcca gttgaagata ttgttgtgga agaattgaaa aaagcttatg tgcgtaaaga
   122101 aatcaattat atgttcaatg gtgatgacgc tggaaatgaa aatcctggtg cattagccaa
   122161 aaaggctgta gcatttaaaa aacctttaga tctaactgct tcaggtgctg ggcaaaaatt
   122221 atatgatgca ttaatcgaat ttaaaaatac accagtgaca gaagtgatga aaaagggacg
   122281 ctttattatt aatcgagctg ctttgactgc cattgaaaaa atgaaaacag atgatggatt
   122341 tcctttgttg cggccattca cacaagcaga aggtggaatt ggttaccaat tagttggcta
   122401 tcctgtggat tggacagatg cagcagataa aaagggtgaa ccagacacgc cagttttata
   122461 ttttggcgat ttttctgcat ttaaaattca agaagttatt ggtgccttgg aaattcaaaa
   122521 acttgttgaa aaattctctg gtaaaaatca aattggattc caaatttaca acttgctaga
   122581 tggtcaattg gtttattctc catttgaacc ggcagtatat cgctacgaaa ttacaaaacc
   122641 agttggtggt taagatggaa gagcaaacta aagaattgtc tttagaggaa aaattcaaat
   122701 cacatattca ttttgaagag ggcatggatg attctttgct ctctttttat ttaaatatgg
   122761 caaaagatta tgtcaaaaca gcaactggtg gccaacaaga atatcttatt ttgatggttg
   122821 ccggcattgc ctatgaatat agagtttcag aggatgaact cgacaaagct atgaatgcca
   122881 tgacgccatt tatcgtgcaa ggagcgattc aaaatgccga agagacagac taataatctc
   122941 agatggaaag ccgaattgct agacatcaaa acaggaacag acggaaacga tcgtccaact
   123001 acaatttacg aatttaagcg tctaatattc tatgaagaac tcggtgtgac ttctcaagaa
   123061 aaatatttat cacagcaagc caagacagac gttgtcagac gaattaaagt cagatgggat
   123121 aaatccatca cagagaaatt aagtgcgctc aaaattgatt ctgtaacgta taacattact
   123181 cgcatttata cgaatcccga tgcaagagaa atggagttga gtttagctta tgtcgattag
   123241 ctttgatgaa ttgaaaacag cgctgaaatc aacaaagtta gcagtgttca gagacaaagc
   123301 cagattaggg acgatgtatc catacattgt gtactcaaat gtgagtaaca gcaaaaaaat
   123361 ggcatccggt aaagtctata aaaaattacc ttattaccaa gtctcttttt ttacacttgg
   123421 aacagaacag gatttagttg ttattgaaca agcgctgcaa aatgctggca ttccatattc
   123481 agattttaca ggtatacaag gtgatgagaa cgacgatacc gtaaccaact attacacata
   123541 tgtgaggtgt gtggaaaatg ccaagtaata agaatggttt ttctgaaata tcggattatt
   123601 taggaaacct ttcaaaagtt gatccgaaaa aattatcgtt ggaatcttta gaagaagcag
   123661 ctaactttta tctaaagcag ttgcttccta atattcctaa gtcgctctta aaaaagaaac
   123721 atatgcgcga tcaaataaaa gtagtagttg aaaacgatca agtgaaagtt caatttgaag
   123781 agacggcctt ttactggcgc tttgccgaaa atggcacaac aaatcaacgt gcccaacatt
   123841 ttgcaagtgg tacgtatgaa caaaacaaag aaaaaattga agaaattatg actagaaaaa
   123901 tacttgattt atgggaaggg tgattttaat tgggaaaaca agatactttt tattttgaag
   123961 gattggacga cattttaatt gcgatgatgg caacgccaga ttctgttgga actgcaccga
   124021 cttatagtga agttttacga ttgccaattg ccacaaaaat cggtgtcaaa gggaatggaa
   124081 cagcattaga aaaatgggca tccagcaaaa tgtttcgtcg tgtgtcgcga gaaacaaaac
   124141 atgaacttgc gttggatcac gtgggtattc cgatagaagt gatggatgaa atcaaaggat
   124201 tagttgctaa aagcggagta acgtttagca aaaataccgc acgagaattt ccgtacttcg
   124261 cctttggatt tattggaaat attgaaaatg gcggtaaaaa agcagtttgg tatcctaata
   124321 cacaactatc caatgtgatt gacgaagagt atgccactgc agaggaagaa acaaaaattg
   124381 acgatgtaac tgcaaatctg gtttccatcg gactgaaata caataatgtg atgtatgcaa
   124441 gttttgattc aaatagagac ggagcttctc tagaattatt taataaattc attgctcaac
   124501 ctgtatacga tgaagaacag tggaaaacat tggctaaagt tggaggtgcg ggttaatggc
   124561 tcggttatct gattatggaa ttcacgttga agacttaaaa aattctgctg ttgtaattat
   124621 tcaaggcgca gaattcccta tttcatttac tatgcaaacg atggaattta tagcagatgt
   124681 gtatggtgga gattattcgc aatttgaatc tgatatgaac gccatgctat ctaaaaaaga
   124741 aggacaaatt tcttctgcta acttatcgcc tagtgatcta aaaatcatgc gtgccttgat
   124801 ttatgccatg ctgcgtactg gtggtttaga ggaagatcca gaaactattt tcaaattttt
   124861 gggaatgagt ggagaggtgt tgtctgctta tagtacctgt atggaaattt ttgctagcca
   124921 gacatttcag gtggaagacc taaaaaaatc caagaagcca caagactttc aaaaagcgca
   124981 agcaaaaaga aagaaaaaca aaaagaatcg gaagagatag gaactccttg gagtttttat
   125041 atttacgttg ctctcactct attgaattgg agtgagagtt tctttttaaa gtctacacct
   125101 aacttgtggc tcaaatctta tttacagtgg ttacaacaaa acaccgattt tgaaccacct
   125161 caatctgtaa ctatggataa atcgccttgg tggtagaaag gagcgctaag ataaatggct
   125221 ggtaaagaat ctgatgtcgt tcttaatttt aaaacgaatg gcgaagtcag ttattcgaaa
   125281 acaatcaaag aaatcaacaa agaaatgaac ttagccgctg ccgagtacaa aaaccaagtg
   125341 tctgcgatgg acaaggatgc aactcaaaca gaaaaattgc gagcggctaa acaaaaatta
   125401 gaaaaacaat taggattggc tgaacaacga tctcaaatgc tgagagagga atatgaaaaa
   125461 tctgtcaaag aaaccggtga atattctgct gaatcagaaa aactttataa acaattactc
   125521 aattctgaaa ccggagaaaa taaacttcgt acagcattag aacagacgaa cgatgcactt
   125581 aaagaacaag gtgacgtttc tgttgataca gcaaaaaaac tccagaaaat cgaagaaaca
   125641 ggtgagaaag taaaaggcgt tggtgaaaaa atgtctgttg gagtaaccgc gcctattgta
   125701 gcggcaggag cagcaggact tgcagcattt ggtgaagttg acgaggcact tgataccatc
   125761 attacaaaaa ccggagcaac aggtgatcaa gctgatagac tttcacagtc tttcaaaaac
   125821 gttggttcaa atactcattt acctttacaa acggttgggg aagctattgg tgaggtaaat
   125881 acacaatttg gattcatgga taaaaaactg gaagattcaa ccaattatct cctacagtac
   125941 gctgaaatca atgatacaga tgtttcgcaa tcagcaatat ctgctcgaca agctattgat
   126001 gcttatggac tagaatatga tgatttgaat tctgtccttg atgtaacaac gaaaacatcg
   126061 cagaatactg gtcaatctgt agacgacttg atgcaaaaag caattgatgg cgcaccacaa
   126121 attaaacaat tagggttgag ctttggtgaa ggggtcactt tacttggaca atttgagcaa
   126181 tctggtgttg attcaagcgc agctttaagt agtttatcta aagcaacagt ggcttatgcg
   126241 aaagacggaa aatctttaag tcaaggacta gaagaactac aagacaaggt aaaaaatgca
   126301 ggttctgaaa cagaagccat caatgcagca gcagaagtat tcgggactaa aggcggtcct
   126361 agaatggccg atgcaattcg tagaggtact ttgaacctgg aagatttagc aaaaactgct
   126421 ggagaaagtg ggggagctgt aggagacaca ttcgatgcta ctctcgaccc aattgatcaa
   126481 gccgatcaag ctatgaacaa tgcaaaatta gcaatggctg acgtaggtga agcagtccaa
   126541 gtaagccttt tgcctttttt tgaaaaagca acttccttat tacaggaatt ctctaaatgg
   126601 tgggggtctc tagatcaaga tacaaagaat tggatcataa caattgctgg tatagcggca
   126661 gcagttggac cagtactaat cgttcttggt tcacttatgg gatctgttac aaaaatcgtt
   126721 ggggaagtta aaagttttat aggagtttgg caaggacttg ctggtttatt tggaatgtcg
   126781 ggaggctggt ttgcattagc agttatagct attggcgcat tagttactgg actaatttgg
   126841 gcatataata acgttaaatg gtttcatgat ggagtgaatt ctttcttcaa aggtgtttca
   126901 gatgtagctg ttgaaatatt taatttttta agtggttatt ttggtggatt tttcgatggt
   126961 gttatagcaa atttcaataa tttttataat gctggaaaac gaattttcag tggttttcta
   127021 gattttatca ctggagtatt tacagggaat tggtcaaaag catggcaagg tctagttgat
   127081 atttttggag gtattttcga tggtattgtt gctgtaggga aagctcctat taatgccatg
   127141 attggattga tcaatggatt tattggtgga ttgaataata taaagatacc aaaatgggtt
   127201 cctggtgtag gtggaaaatc attttctatt tcaaaactgc cttatttagc acaaggcggt
   127261 catctaatca atggacaagc gattgttggt gaagctggac ctgagttact gactgccaag
   127321 aatggtaaaa ccacagttac tccattatca gacgaagaaa aacgcaaagg gatcagtgga
   127381 agagtttctg gaggtagcat tgaacagcat atacacattg gcaaagtcga tgcaaacaat
   127441 ccatctgagt tagatcgtat gaatcgtaaa tttgctaaag caaacaaaca ggctattcgt
   127501 gatttgggag gtgttcctat ttgagtcgac aatttatgaa tccagatgaa ccaaatttta
   127561 tttggaaaaa tcggaatgca gtaattgata tgaattgcat cattgaggca gagcttccag
   127621 atattatgcc gaacaagcga tacgaaactt acacgattca gggccgaagc ggagaattga
   127681 cggagacgtt tgatgactat gaaccttttg atttggaaat agaagggatc actattccac
   127741 attcgaaact gagagaagtc aaacgatggc tcactggtaa aagccaatta atcacacata
   127801 atgatccaga caagtatcta gaggctatct gcagcatgga taaagaagtc cctttcgaaa
   127861 acgagtgggg cttcttttat acattcgatg ttacttttcg ttgtcagcca ctcaaaagaa
   127921 aaatcggtga agcacctaaa atttttcatt ctagtgttat ggatattttt gatcatggtg
   127981 acgaaattgc acatccctat ctggaaatag aatcgaacgg aggagatata acgatcgctg
   128041 tcggtgataa aagcctaaca atacttaata cgttagctgc aacaatcgtg gttgatactc
   128101 agcttggaaa agcaatacaa gaaggtttga atttatttac gcgtggtgat tggccagtat
   128161 tgcagcctga atggaatcag gtaaaaatat ccggaaaatt taaagaagtg cggttttgga
   128221 ataggagtgt gtatctgtga cgcaagaatt tatttatgct tataaaaaaa tgccagatga
   128281 tttgagcgtg aacggagcgt cgttagttga ttgggaagat ttgccagaaa tcaatcgtgt
   128341 gctgaatggc caatatcgtt tttatggtaa ctattcaaga agcggacagt atcgctctta
   128401 tttaaaaaaa ggaaatttta taaaagcgaa ggtaccagat ggatcatggc aatattttga
   128461 aatttataat gtcaaaaaga atttgacctc cgtttctgta acagctcggc atattggttt
   128521 tatggccaat aagaacttta ttgtcaagtc gtttacggat aacggcaatg ggtctcagat
   128581 catgaccaat cttaaaaaca gtttggcatt tgatcaaaaa tttaactatc tttcgaatgt
   128641 agggacaacg caccaattta cagcaagaca agtcgcgcca gttgaagcaa tcattggttc
   128701 aaataacggt aatcaaaatt tgactggcgt gactagcgcg gaacttgata tggataacta
   128761 tgatttgaaa ctggtaaaac agattggatc agataatggt tttcggattg attttggcat
   128821 caatttagaa gcaatcgaag aagaaattga tgaagaatca attgtcaata gtttgtattt
   128881 ggttggcggt gttcctgata acgattatga tgaagacaaa gaacctatcg aatatggtta
   128941 tttagaaatt gatggagtaa ccaacgagaa tcgacgtatt gccaaacgtg aaaacggaga
   129001 ttgtaaaaca gttgatgaat tgatcaaatg gggcaaaacc ttatttgaca atgatcgtat
   129061 ccatgagcca aaagctactc atacagtcag tatggtggcg ttggaacaca cactcgaata
   129121 cggtgacatg tatcaagagc tagcgtcttt acactttggt gatgtcgctc atgttagggc
   129181 gaaagagcta gatatcgaga taaaagagcg tatggtcgaa tacacttatt tcccaacgct
   129241 tgggaaatat aaggatattg ttttaggtaa tgatttatca ctgtacactt cggcagtaaa
   129301 tactcaagca caagaattga aaaagaaaat cgacaatcga acagaaacgt tagttcaaaa
   129361 tgtgttgaat gcgacggcat ggatcactgg taattctggt gggcatgtcg tttttcgtcc
   129421 agaaaaagct ccatcagaga ttcttatcat ggatactcca aatgtggcga atgcaaagcg
   129481 agtgtggcgt tggaatttaa atggcttagg ttattcagac aacggagtaa atggcccgtt
   129541 cggtattgct atcacatcaa aaggagaaat tgttgctgac ttcatcaaag taggaacaat
   129601 aaatgcagaa gtattcgaaa cttcctttaa tgcttatggt gatgtgttaa aacttgtaaa
   129661 aggcacgctt caaatttgga atgaaaacaa aaaaatcatg gagctaacca aaaaaggtat
   129721 ggaattctgg aatagaaaag aatccattgg aacgatcgga acaacagatt cagcaggaaa
   129781 tccatttcca aatgctgtaa cacctacacc tcttgaggaa aattctttgg taatacgtac
   129841 aaatggcgat ggaaaataca ttttaatttc tccaactgca gaaaaaggat ttgtgttatt
   129901 aggaaacgga aaagcatatt atttcggtga tttagatatt cagggaaaac ttactgttag
   129961 aggtaaagaa gtgataccag gacaaaatgg cggtccatct ggcggaggag aaacacctgg
   130021 tgggtatcct gatgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DANKHJ010000002        93136 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000002 DANKHJ010000000
VERSION     DANKHJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..420
                     /locus_tag="PW113_000152"
     CDS             <1..420
                     /locus_tag="PW113_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2524495.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="PW113_000153"
     CDS             complement(504..1226)
                     /locus_tag="PW113_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2524496.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="PW113_000154"
     CDS             complement(1216..1953)
                     /locus_tag="PW113_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524497.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="PW113_000155"
     CDS             complement(1965..2957)
                     /locus_tag="PW113_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2524498.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="PW113_000156"
     CDS             complement(2972..3715)
                     /locus_tag="PW113_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524499.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="PW113_000157"
     CDS             complement(3956..5278)
                     /locus_tag="PW113_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2524500.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="PW113_000158"
     CDS             complement(5265..6230)
                     /locus_tag="PW113_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524501.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="PW113_000159"
     CDS             complement(6227..7765)
                     /locus_tag="PW113_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2524502.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="PW113_000160"
     CDS             complement(8181..8468)
                     /locus_tag="PW113_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524503.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="PW113_000161"
     CDS             8602..9270
                     /locus_tag="PW113_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2524504.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="PW113_000162"
     CDS             complement(9561..10301)
                     /locus_tag="PW113_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524505.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="PW113_000163"
     CDS             complement(10431..11378)
                     /locus_tag="PW113_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2524506.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="PW113_000164"
     CDS             complement(11507..12271)
                     /locus_tag="PW113_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2524507.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="PW113_000165"
     CDS             complement(12275..12664)
                     /locus_tag="PW113_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524508.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="PW113_000166"
     CDS             12799..13341
                     /locus_tag="PW113_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2524509.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="PW113_000167"
     CDS             13489..14121
                     /locus_tag="PW113_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524510.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="PW113_000168"
     CDS             complement(14255..14380)
                     /locus_tag="PW113_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2524511.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="PW113_000169"
     CDS             complement(14514..15407)
                     /locus_tag="PW113_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2524512.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="PW113_000170"
     CDS             complement(15589..16515)
                     /locus_tag="PW113_000170"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2524513.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PW113_000171"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PW113_000171"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2524514.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PW113_000172"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PW113_000172"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2524515.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PW113_000173"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PW113_000173"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2524516.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PW113_000174"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PW113_000174"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2524517.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="PW113_000175"
     CDS             complement(24786..25220)
                     /locus_tag="PW113_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2524518.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="PW113_000176"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="PW113_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2524519.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="PW113_000177"
     CDS             complement(26117..26992)
                     /locus_tag="PW113_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2524520.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PW113_000178"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PW113_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2524521.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PW113_000179"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PW113_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2524522.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PW113_000180"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PW113_000180"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2524523.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PW113_000181"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PW113_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2524524.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PW113_000182"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PW113_000182"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2524525.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PW113_000183"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PW113_000183"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2524526.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRYDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PW113_000184"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PW113_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2524527.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="PW113_000185"
     CDS             complement(35443..36210)
                     /locus_tag="PW113_000185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2524528.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PW113_000186"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PW113_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2524529.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="PW113_000187"
     CDS             37323..37721
                     /locus_tag="PW113_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2524530.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            38000..38500
                     /locus_tag="PW113_000188"
     CDS             38000..38500
                     /locus_tag="PW113_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2524531.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PW113_000189"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PW113_000189"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2524532.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="PW113_000190"
     CDS             complement(39286..39513)
                     /locus_tag="PW113_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2524533.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PW113_000191"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PW113_000191"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2524534.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="PW113_000192"
     CDS             complement(40048..40761)
                     /locus_tag="PW113_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2524535.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="PW113_000193"
     CDS             complement(40764..41660)
                     /locus_tag="PW113_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2524536.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="PW113_000194"
     CDS             complement(41818..42660)
                     /locus_tag="PW113_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2524537.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="PW113_000195"
     CDS             42792..43445
                     /locus_tag="PW113_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2524538.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="PW113_000196"
     CDS             complement(43508..44035)
                     /locus_tag="PW113_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2524539.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="PW113_000197"
     CDS             complement(44055..45002)
                     /locus_tag="PW113_000197"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2524540.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="PW113_000198"
     CDS             complement(45014..46900)
                     /locus_tag="PW113_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2524541.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="PW113_000199"
     CDS             complement(47060..49138)
                     /locus_tag="PW113_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2524542.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="PW113_000200"
     CDS             complement(49151..49426)
                     /locus_tag="PW113_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524543.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="PW113_000201"
     CDS             complement(49788..50243)
                     /locus_tag="PW113_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524544.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="PW113_000202"
     CDS             complement(50397..51605)
                     /locus_tag="PW113_000202"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2524545.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="PW113_000203"
     CDS             complement(51717..52049)
                     /locus_tag="PW113_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2524546.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="PW113_000204"
     CDS             52197..53072
                     /locus_tag="PW113_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2524547.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="PW113_000205"
     CDS             complement(53154..53735)
                     /locus_tag="PW113_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2524548.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="PW113_000206"
     CDS             complement(53741..55000)
                     /locus_tag="PW113_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2524549.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="PW113_000207"
     CDS             complement(55002..56048)
                     /locus_tag="PW113_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524550.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="PW113_000208"
     CDS             complement(56283..56558)
                     /locus_tag="PW113_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2524551.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="PW113_000209"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="PW113_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2524552.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PW113_000210"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PW113_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2524553.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="PW113_000211"
     CDS             complement(59657..60337)
                     /locus_tag="PW113_000211"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2524554.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="PW113_000212"
     CDS             complement(60386..61021)
                     /locus_tag="PW113_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2524555.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="PW113_000213"
     CDS             complement(61087..62490)
                     /locus_tag="PW113_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2524556.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="PW113_000214"
     CDS             complement(62477..63508)
                     /locus_tag="PW113_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2524557.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="PW113_000215"
     CDS             63539..63760
                     /locus_tag="PW113_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2524558.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="PW113_000216"
     CDS             complement(63925..64662)
                     /locus_tag="PW113_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL2524559.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="PW113_000217"
     CDS             complement(64815..66155)
                     /locus_tag="PW113_000217"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2524560.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="PW113_000218"
     CDS             complement(66392..67915)
                     /locus_tag="PW113_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2524561.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="PW113_000219"
     CDS             complement(68293..68904)
                     /locus_tag="PW113_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2524562.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="PW113_000220"
     CDS             complement(69234..69950)
                     /locus_tag="PW113_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2524563.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PW113_000221"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PW113_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2524564.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71306)
                     /locus_tag="PW113_000222"
     CDS             complement(70509..71306)
                     /locus_tag="PW113_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2524565.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="PW113_000223"
     CDS             complement(71276..71647)
                     /locus_tag="PW113_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2524566.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PW113_000224"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PW113_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2524567.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="PW113_000225"
     CDS             complement(72649..73122)
                     /locus_tag="PW113_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2524568.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="PW113_000226"
     CDS             complement(73227..74090)
                     /locus_tag="PW113_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2524569.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="PW113_000227"
     CDS             complement(74286..76385)
                     /locus_tag="PW113_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2524570.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="PW113_000228"
     CDS             complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="PW113_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2524571.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77738..79579)
                     /locus_tag="PW113_000229"
     CDS             complement(77738..79579)
                     /locus_tag="PW113_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2524572.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79761..82076)
                     /locus_tag="PW113_000230"
     CDS             complement(79761..82076)
                     /locus_tag="PW113_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2524573.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82261..83121)
                     /locus_tag="PW113_000231"
     CDS             complement(82261..83121)
                     /locus_tag="PW113_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2524574.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83180..83701)
                     /locus_tag="PW113_000232"
     CDS             complement(83180..83701)
                     /locus_tag="PW113_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2524575.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            83979..84740
                     /locus_tag="PW113_000233"
     CDS             83979..84740
                     /locus_tag="PW113_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2524576.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            84766..87357
                     /gene="mprF"
                     /locus_tag="PW113_000234"
     CDS             84766..87357
                     /gene="mprF"
                     /locus_tag="PW113_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2524577.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="PW113_000235"
     CDS             complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="PW113_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2524578.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            89100..89456
                     /locus_tag="PW113_000236"
     CDS             89100..89456
                     /locus_tag="PW113_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2524579.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(89697..90701)
                     /locus_tag="PW113_000237"
     CDS             complement(89697..90701)
                     /locus_tag="PW113_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2524580.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            90867..91718
                     /locus_tag="PW113_000238"
     CDS             90867..91718
                     /locus_tag="PW113_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2524581.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            91735..91980
                     /locus_tag="PW113_000239"
     CDS             91735..91980
                     /locus_tag="PW113_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524582.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            92167..92871
                     /locus_tag="PW113_000240"
     CDS             92167..92871
                     /locus_tag="PW113_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524583.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        25866 a        19521 c        15977 g        31772 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagttgtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 atctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    76561 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    76621 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    76681 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    76741 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    76801 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    76861 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    76921 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    76981 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    77041 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    77101 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    77161 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    77221 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    77281 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    77341 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    77401 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    77461 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    77521 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    77581 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    77641 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    77701 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    77761 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    77821 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    77881 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    77941 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    78001 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    78061 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    78121 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    78181 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    78241 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    78301 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    78361 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    78421 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    78481 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    78541 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    78601 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    78661 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    78721 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    78781 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    78841 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    78901 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    78961 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    79021 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    79081 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    79141 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    79201 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag
    79261 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    79321 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    79381 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    79441 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    79501 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    79561 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    79621 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    79681 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    79741 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    79801 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    79861 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    79921 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    79981 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    80041 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    80101 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    80161 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    80221 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    80281 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    80341 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    80401 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    80461 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    80521 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    80581 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    80641 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    80701 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    80761 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    80821 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    80881 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    80941 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    81001 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    81061 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    81121 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    81181 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    81241 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    81301 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    81361 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    81421 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    81481 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    81541 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    81601 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    81661 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    81721 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    81781 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    81841 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    81901 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    81961 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    82021 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    82081 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    82141 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    82201 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    82261 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    82321 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    82381 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    82441 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    82501 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    82561 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    82621 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    82681 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    82741 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    82801 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    82861 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    82921 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    82981 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    83041 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    83101 cattttttgc cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga
    83161 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    83221 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    83281 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    83341 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    83401 gattttttac ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata
    83461 aatcgcatcc ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat
    83521 caatactgtc agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct
    83581 gcatgagatc tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct
    83641 cgtagttttc catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca
    83701 ctcgttttac cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca
    83761 gaatgatcat gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac
    83821 ctagttattt tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc
    83881 cttgatttct gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac
    83941 gaaaatgatc gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac
    84001 tttacacagt aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga
    84061 gcgaaattta ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg
    84121 acggcagcgg ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt
    84181 atctagtaga tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc
    84241 agttaatggc cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt
    84301 ttcttggatt tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa
    84361 aagttcatcg attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg
    84421 ccagagtcat cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa
    84481 gtcttcgcaa aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa
    84541 atgatttaaa aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca
    84601 agctaaaaca ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga
    84661 aagaacaagg acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc
    84721 aatttctttc tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc
    84781 atacaatggc tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt
    84841 gtcattattg tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga
    84901 gaaacgtttg ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc
    84961 tctgtgttac ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag
    85021 aatcctcgtt acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt
    85081 ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc
    85141 agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt
    85201 tacagtctgc tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa
    85261 tattggatat ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact
    85321 ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta
    85381 gtttcattgt tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg
    85441 gaaatccgga ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga
    85501 atcgtgtcta tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg
    85561 tccgctattg gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc
    85621 tactacatca ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact
    85681 tttgatcagc gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc
    85741 gtggttgtct tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa
    85801 gcttttacag agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc
    85861 caatttccta gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca
    85921 cgtgtgaaac gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt
    85981 ttattaagtg attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt
    86041 cttgcttcta aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg
    86101 atcgatggaa tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat
    86161 ctgccggatt tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa
    86221 aagatttggt tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt
    86281 gttcatttcc ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc
    86341 ctgaagatcc ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat
    86401 aagcggatgt ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc
    86461 ttcaataata aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa
    86521 gcaatcgaag cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa
    86581 acaagcgaag agattgttat gattttgcat gaattcggct acgatttcat taagatgggg
    86641 gaagaagctt atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact
    86701 cgtgctgtct tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca
    86761 ttttctactg agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga
    86821 aaggaaaaag ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt
    86881 gcagttgtca aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac
    86941 acgaacaata aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct
    87001 ggcagtatgg atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa
    87061 tggttcaatt tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta
    87121 caggaaagga ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga
    87181 ttaagagaat ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct
    87241 agagatagtt ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt
    87301 gagcagacac aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa
    87361 ctgcataccg atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag
    87421 ttcatgcttc tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa
    87481 tcctgtttgg accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg
    87541 tttcttatgc ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa
    87601 ttgagaatgt cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca
    87661 cgttgtcacc aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat
    87721 cagttgtgcg gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac
    87781 cttcttcttt tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac
    87841 ctggtttagc aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca
    87901 cgcctacgtt atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg
    87961 ttacaactgc tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac
    88021 gaacagcacc acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga
    88081 cgtcttccac tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt
    88141 catctactgt atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg
    88201 cttttaatgc ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca
    88261 ataactcgcg aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa
    88321 ctttgttcaa gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt
    88381 cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt
    88441 gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg
    88501 cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt
    88561 cacgttcttc tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt
    88621 tgcctaatac tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa
    88681 tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca
    88741 aacctcctaa ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct
    88801 ttttttgact aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc
    88861 ttattatacg tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa
    88921 aaaagtacct tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt
    88981 aactttcttt tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca
    89041 acaactttca atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca
    89101 tgaaaaaatt aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag
    89161 cttatcatta tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa
    89221 tgtcagctac ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg
    89281 cttataataa agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat
    89341 taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg
    89401 aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa
    89461 tggtcttaaa attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa
    89521 taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg
    89581 ttctttactt ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt
    89641 gacacaattc atttaactga tcactggtac ttgaacacct aatattctag tcatttttat
    89701 aaacgattca atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta
    89761 ttcacaaatt caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc
    89821 ccgtttgata gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct
    89881 tttacaggag gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt
    89941 tttgccgtga tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct
    90001 ttttcttcta tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct
    90061 gtctcaaatt tttttccaag atgcttggct actttttgga tcacttttac attttcttct
    90121 aaagaaggtg caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca
    90181 gaaaactcat attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat
    90241 gcttcatctg ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg
    90301 tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc
    90361 aataaagata agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta
    90421 ggtgcttctt cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct
    90481 tccacaaaat tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt
    90541 tgtccagttt ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga
    90601 atatattctt ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg
    90661 tcaataatat gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc
    90721 ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa
    90781 atttcaagag tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt
    90841 gaacgaataa aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa
    90901 atggttacga aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg
    90961 ctgtttcctc tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag
    91021 gatacaagaa tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag
    91081 agatattctt gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt
    91141 cttttgaaga atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact
    91201 ggccaaaccc tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag
    91261 catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac
    91321 cccaccatct agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt
    91381 tccttgcacc aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga
    91441 tcctcgaagc atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac
    91501 aaatcgctga agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac
    91561 acgaattcct tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat
    91621 tatttgattt tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag
    91681 tcggcaaagc aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat
    91741 ccaatcgaac gcttgaattc tctctcggaa gaagttaccc agacttttca ttccgatttt
    91801 gtctttttga tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat
    91861 caaatcatcg aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac
    91921 gaacatgaag tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag
    91981 aaagaatgga acggattatg tatatataca taatccgttc cattttttat ttttctttca
    92041 aaaaacggga aaatacgcaa atttttctgt tgtaatttgc gtatacgcaa gtttttttgc
    92101 attactcctt ttcatttggc tgtttttttc ttataatcga aagggtatga gcgaaggaga
    92161 gaacatatga agaaaaaaca gaaacaaaaa ttattaaatc aattccgtcc ttcattggat
    92221 ggtgtaagag gacaattatt ccgttctcta gaagaagaga gcttgcgacg ttatggtttg
    92281 ccgttacaag tcatgcttga tcccaaaaac cgtcaaatct taacgatcgg attagctggg
    92341 aaaacaaatg aagatacacg gatcaacgtg ccaatcgatg acaacttcaa tactgtatta
    92401 aagagaattc gttcaggaga aaacgggatt ttcgaacgat tccgtgacaa tcttcttatt
    92461 gaaatcgtgt cttactggaa tgatcaacga ttgaaaaacg atgaaccaac tgcgcaagct
    92521 gttgcgacac cagttacaga agaaacagtc agtgtgccag aagaaaaaac ggaaaaacca
    92581 aaagcggaca caactgaaaa tacacgctca actgatacga atgccgcttt aagttttgct
    92641 gatttttcaa aagccgttgc tgagtatcct aaattttaca cagaacaaaa tgaacatgaa
    92701 gtagttatta atgaaaaaaa tgctgatgaa cctcgtaaat tagcaactat ttctatgact
    92761 actgaaaatg agttcgttat cgaaaaagca ctcgaacgga aatacaaagt caaattgact
    92821 ttgattcctt tgatcgaaca atttgcagcg acagcgatta ctgatcgata aaaattctat
    92881 ttgtcataaa aggctgggat ttaaaatccc agccttttta gtcatataat aatttctaca
    92941 gcaagtaaaa agaaattcct tctaacatca ggctcaaata tcagcttgac ctgtccaaca
    93001 aatcacctat gatcctacaa tgttaggcga cttctcttct acctgaagat atcttggagt
    93061 tgcctgattt cttaagatgc ctaaccttaa ctataaaaaa ttctaataat aaacatattt
    93121 tctgatagag aaaaaa
//
LOCUS       DANKHJ010000003        92018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000003 DANKHJ010000000
VERSION     DANKHJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(551..2164)
                     /locus_tag="PW113_000241"
     CDS             complement(551..2164)
                     /locus_tag="PW113_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2524584.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="PW113_000242"
     CDS             complement(2305..3213)
                     /locus_tag="PW113_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2524585.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="PW113_000243"
     CDS             3396..4520
                     /locus_tag="PW113_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2524586.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4545..4724
                     /locus_tag="PW113_000244"
     CDS             4545..4724
                     /locus_tag="PW113_000244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524587.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4747..5664
                     /locus_tag="PW113_000245"
     CDS             4747..5664
                     /locus_tag="PW113_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2524588.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="PW113_000246"
     CDS             5657..6490
                     /locus_tag="PW113_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2524589.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="PW113_000247"
     CDS             6573..7691
                     /locus_tag="PW113_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2524590.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7685..9583
                     /locus_tag="PW113_000248"
     CDS             7685..9583
                     /locus_tag="PW113_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2524591.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9577..10800
                     /locus_tag="PW113_000249"
     CDS             9577..10800
                     /locus_tag="PW113_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2524592.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10886..12820)
                     /locus_tag="PW113_000250"
     CDS             complement(10886..12820)
                     /locus_tag="PW113_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2524593.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13046..13702
                     /locus_tag="PW113_000251"
     CDS             13046..13702
                     /locus_tag="PW113_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2524594.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13776..14129)
                     /locus_tag="PW113_000252"
     CDS             complement(13776..14129)
                     /locus_tag="PW113_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2524595.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14321..15250
                     /locus_tag="PW113_000253"
     CDS             14321..15250
                     /locus_tag="PW113_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2524596.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15261..16097
                     /locus_tag="PW113_000254"
     CDS             15261..16097
                     /locus_tag="PW113_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2524597.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16345..17121
                     /locus_tag="PW113_000255"
     CDS             16345..17121
                     /locus_tag="PW113_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2524598.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17297..18040
                     /locus_tag="PW113_000256"
     CDS             17297..18040
                     /locus_tag="PW113_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524599.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18033..19613
                     /locus_tag="PW113_000257"
     CDS             18033..19613
                     /locus_tag="PW113_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2524600.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19864..20343)
                     /locus_tag="PW113_000258"
     CDS             complement(19864..20343)
                     /locus_tag="PW113_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2524601.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20359..21111)
                     /locus_tag="PW113_000259"
     CDS             complement(20359..21111)
                     /locus_tag="PW113_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2524602.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21228..21298
                     /locus_tag="PW113_000260"
     tRNA            21228..21298
                     /locus_tag="PW113_000260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21260..21262,aa:Gly,seq:ccc)
     gene            21659..22384
                     /locus_tag="PW113_000261"
     CDS             21659..22384
                     /locus_tag="PW113_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2524603.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22639..22905
                     /locus_tag="PW113_000262"
     CDS             22639..22905
                     /locus_tag="PW113_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2524604.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22902..23333
                     /gene="ruvX"
                     /locus_tag="PW113_000263"
     CDS             22902..23333
                     /gene="ruvX"
                     /locus_tag="PW113_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2524605.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23358..23663
                     /locus_tag="PW113_000264"
     CDS             23358..23663
                     /locus_tag="PW113_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2524606.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23744..24889)
                     /locus_tag="PW113_000265"
     CDS             complement(23744..24889)
                     /locus_tag="PW113_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2524607.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24950..25597)
                     /locus_tag="PW113_000266"
     CDS             complement(24950..25597)
                     /locus_tag="PW113_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2524608.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25791..26084
                     /locus_tag="PW113_000267"
     CDS             25791..26084
                     /locus_tag="PW113_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524609.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26128..26439
                     /locus_tag="PW113_000268"
     CDS             26128..26439
                     /locus_tag="PW113_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524610.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26436..27060
                     /locus_tag="PW113_000269"
                     /pseudo
     CDS             26436..27060
                     /locus_tag="PW113_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27201..27359
                     /locus_tag="PW113_000270"
     CDS             27201..27359
                     /locus_tag="PW113_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524611.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27360..27722
                     /locus_tag="PW113_000271"
     CDS             27360..27722
                     /locus_tag="PW113_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524612.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27759..28607
                     /locus_tag="PW113_000272"
     CDS             27759..28607
                     /locus_tag="PW113_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2524613.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28597..30072
                     /locus_tag="PW113_000273"
     CDS             28597..30072
                     /locus_tag="PW113_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2524614.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30338..30745
                     /locus_tag="PW113_000274"
     CDS             30338..30745
                     /locus_tag="PW113_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2524615.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30748..30963
                     /locus_tag="PW113_000275"
     CDS             30748..30963
                     /locus_tag="PW113_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524616.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30967..31347
                     /locus_tag="PW113_000276"
     CDS             30967..31347
                     /locus_tag="PW113_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2524617.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31481..31636
                     /locus_tag="PW113_000277"
     CDS             31481..31636
                     /locus_tag="PW113_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2524618.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31705..32178
                     /locus_tag="PW113_000278"
     CDS             31705..32178
                     /locus_tag="PW113_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2524619.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32175..33869
                     /locus_tag="PW113_000279"
     CDS             32175..33869
                     /locus_tag="PW113_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2524620.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33835..34020
                     /locus_tag="PW113_000280"
     CDS             33835..34020
                     /locus_tag="PW113_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524621.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34024..35199
                     /locus_tag="PW113_000281"
     CDS             34024..35199
                     /locus_tag="PW113_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2524622.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35192..36715
                     /locus_tag="PW113_000282"
     CDS             35192..36715
                     /locus_tag="PW113_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2524623.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36771..37055
                     /locus_tag="PW113_000283"
     CDS             36771..37055
                     /locus_tag="PW113_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2524624.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37042..37377
                     /locus_tag="PW113_000284"
     CDS             37042..37377
                     /locus_tag="PW113_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2524625.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37672..38298
                     /locus_tag="PW113_000285"
     CDS             37672..38298
                     /locus_tag="PW113_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524626.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39040..39876
                     /locus_tag="PW113_000286"
     CDS             39040..39876
                     /locus_tag="PW113_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2524627.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39940..40371
                     /locus_tag="PW113_000287"
     CDS             39940..40371
                     /locus_tag="PW113_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2524628.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40433..41755)
                     /locus_tag="PW113_000288"
     CDS             complement(40433..41755)
                     /locus_tag="PW113_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2524629.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41909..43549)
                     /locus_tag="PW113_000289"
     CDS             complement(41909..43549)
                     /locus_tag="PW113_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2524630.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43882..44352
                     /locus_tag="PW113_000290"
     CDS             43882..44352
                     /locus_tag="PW113_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2524631.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44574..45677
                     /locus_tag="PW113_000291"
     CDS             44574..45677
                     /locus_tag="PW113_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2524632.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMTPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45961..46641
                     /locus_tag="PW113_000292"
     CDS             45961..46641
                     /locus_tag="PW113_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2524633.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46731..47765
                     /locus_tag="PW113_000293"
     CDS             46731..47765
                     /locus_tag="PW113_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2524634.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47779..48114
                     /locus_tag="PW113_000294"
     CDS             47779..48114
                     /locus_tag="PW113_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524635.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48098..50578
                     /locus_tag="PW113_000295"
     CDS             48098..50578
                     /locus_tag="PW113_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524636.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50634..52193
                     /locus_tag="PW113_000296"
     CDS             50634..52193
                     /locus_tag="PW113_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2524637.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52190..53554
                     /locus_tag="PW113_000297"
     CDS             52190..53554
                     /locus_tag="PW113_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2524638.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53725..54795
                     /locus_tag="PW113_000298"
     CDS             53725..54795
                     /locus_tag="PW113_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524639.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54810..55478
                     /locus_tag="PW113_000299"
     CDS             54810..55478
                     /locus_tag="PW113_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524640.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55498..56076
                     /locus_tag="PW113_000300"
     CDS             55498..56076
                     /locus_tag="PW113_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2524641.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56477..57388
                     /locus_tag="PW113_000301"
     CDS             56477..57388
                     /locus_tag="PW113_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2524642.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57766..58683)
                     /locus_tag="PW113_000302"
     CDS             complement(57766..58683)
                     /locus_tag="PW113_000302"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2524643.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58885..59322
                     /gene="zapA"
                     /locus_tag="PW113_000303"
     CDS             58885..59322
                     /gene="zapA"
                     /locus_tag="PW113_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2524644.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59380..59928
                     /locus_tag="PW113_000304"
     CDS             59380..59928
                     /locus_tag="PW113_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2524645.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60043..62403
                     /locus_tag="PW113_000305"
     CDS             60043..62403
                     /locus_tag="PW113_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2524646.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62490..62804
                     /gene="trxA"
                     /locus_tag="PW113_000306"
     CDS             62490..62804
                     /gene="trxA"
                     /locus_tag="PW113_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2524647.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62986..64764
                     /gene="uvrC"
                     /locus_tag="PW113_000307"
     CDS             62986..64764
                     /gene="uvrC"
                     /locus_tag="PW113_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2524648.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64909..65808
                     /locus_tag="PW113_000308"
     CDS             64909..65808
                     /locus_tag="PW113_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524649.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65801..67063
                     /locus_tag="PW113_000309"
     CDS             65801..67063
                     /locus_tag="PW113_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524650.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67224..69122
                     /locus_tag="PW113_000310"
     CDS             67224..69122
                     /locus_tag="PW113_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL2524651.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69452..70171)
                     /locus_tag="PW113_000311"
     CDS             complement(69452..70171)
                     /locus_tag="PW113_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2524652.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            70508..70840
                     /locus_tag="PW113_000312"
     CDS             70508..70840
                     /locus_tag="PW113_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524653.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70827..72830
                     /locus_tag="PW113_000313"
     CDS             70827..72830
                     /locus_tag="PW113_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2524654.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72849..73319
                     /locus_tag="PW113_000314"
     CDS             72849..73319
                     /locus_tag="PW113_000314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2524655.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73340..73924
                     /locus_tag="PW113_000315"
     CDS             73340..73924
                     /locus_tag="PW113_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2524656.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73939..74925
                     /locus_tag="PW113_000316"
     CDS             73939..74925
                     /locus_tag="PW113_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2524657.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74915..75226
                     /locus_tag="PW113_000317"
     CDS             74915..75226
                     /locus_tag="PW113_000317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2524658.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75248..77029
                     /locus_tag="PW113_000318"
     CDS             75248..77029
                     /locus_tag="PW113_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2524659.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77022..78398
                     /locus_tag="PW113_000319"
     CDS             77022..78398
                     /locus_tag="PW113_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2524660.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78405..79040
                     /locus_tag="PW113_000320"
     CDS             78405..79040
                     /locus_tag="PW113_000320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2524661.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79070..80425
                     /locus_tag="PW113_000321"
     CDS             79070..80425
                     /locus_tag="PW113_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2524662.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80617..82011)
                     /locus_tag="PW113_000322"
     CDS             complement(80617..82011)
                     /locus_tag="PW113_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2524663.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82103..83074
                     /locus_tag="PW113_000323"
     CDS             82103..83074
                     /locus_tag="PW113_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2524664.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83071..83853
                     /locus_tag="PW113_000324"
     CDS             83071..83853
                     /locus_tag="PW113_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2524665.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84022..84393)
                     /locus_tag="PW113_000325"
     CDS             complement(84022..84393)
                     /locus_tag="PW113_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2524666.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84527..84970
                     /locus_tag="PW113_000326"
     CDS             84527..84970
                     /locus_tag="PW113_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2524667.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85013..85822)
                     /gene="yidA"
                     /locus_tag="PW113_000327"
     CDS             complement(85013..85822)
                     /gene="yidA"
                     /locus_tag="PW113_000327"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2524668.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85841..87208)
                     /locus_tag="PW113_000328"
     CDS             complement(85841..87208)
                     /locus_tag="PW113_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2524669.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87192..88022)
                     /locus_tag="PW113_000329"
     CDS             complement(87192..88022)
                     /locus_tag="PW113_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2524670.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88185..88622
                     /locus_tag="PW113_000330"
     CDS             88185..88622
                     /locus_tag="PW113_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2524671.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88698..89318
                     /locus_tag="PW113_000331"
     CDS             88698..89318
                     /locus_tag="PW113_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2524672.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89507..90013
                     /locus_tag="PW113_000332"
     CDS             89507..90013
                     /locus_tag="PW113_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2524673.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90030..90929
                     /locus_tag="PW113_000333"
     CDS             90030..90929
                     /locus_tag="PW113_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2524674.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91016..91900
                     /locus_tag="PW113_000334"
     CDS             91016..91900
                     /locus_tag="PW113_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2524675.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        30403 a        15609 c        18939 g        27067 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgcaaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tgcaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagttttagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaacgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaattcc aagcttagtt
    10921 cttcccattc cgtcaatttt gtttcttgat cttgtctagc agcttctaaa tcttggttca
    10981 gctcattgag ttttacgtga tcttccaggt tttctggcgc gaccatttct tgttccaact
    11041 gatcgatcca ttcatcgatc cgcgccattt cttcttcaac ctgagtaatt tttcgttgaa
    11101 ggctacggat aagcttttgc tgttctttgc tttggtaata atcagatttg ttcgtgggag
    11161 caggagcaga ttctggctca tcttgtgcca acaattcggc gatttcttct tcttcctgct
    11221 ttttctctaa ataataatca tagtctccta gatagacttt gctgccattt tcagagagtt
    11281 cgacgacttt tgtcgcgatc cgattgatga aatatcggtc atgggaaaca aaaagcagtg
    11341 tgccgtcata atcgatcaat gcattttcta atacttcttt gttgtcaata tctaaatggt
    11401 ttgttggttc atccaaaatc aaaaaatttt ctttgttcat cgctaacttg gctaaagcaa
    11461 cccgtgcttt ttcaccgcca cttagcagag ggattggttt ttctacatct tcgccagaaa
    11521 ataagaaagc acctaaagca cttcggatct ctttttctgg agtggtagga tgctcatccc
    11581 acaattctgc taggatcgtc ttgtttgtat gcagatcagc ttgaccttga tcgtaatagc
    11641 cgatggtgac atttgtccct agcgtttctt ttccacggat aaaagggatc tggtgaatga
    11701 ttgatttcag caacgtcgat ttcccgatac cattcggacc tactagagca atcgcttctt
    11761 gacgacggat atctagattg attggttcag ataaggtgac attatcatat ccgatagcgg
    11821 catcttcgat ttgcaagact acatttccag aaggcttttc tatactgaat agaaagtttg
    11881 cttgtttttc atccccttga ggacgatcca gccggtccat tttttctaat gtctttctgc
    11941 ggctttgcgc tcgttttgtg gtagaggcgc gaacaaggtt ccgagcaacg aagtcttcta
    12001 atttatggat ttctgtttgc tgcttttcat aagccttcca ttcacttgct aactgttctg
    12061 ctttcaaatc tagatatttc gaatagttac ctttgtaatg gcgcattttt cttctgctta
    12121 attcatagac ttcattgact actttatcta aaaaataacg gtcatgggaa acaatcaata
    12181 atgcaccagt atagctttgc agataattct caagccagga aagtgtctcg atatctaaat
    12241 ggttcgttgg ttcatctaga atcaaaatgt cagggtgttg caaaagcatg cgagctaaag
    12301 ctaaacgtgt gcgctgtccg cctgataaag tatcgatttt ctgatcataa aatgaagaat
    12361 cgaatttaaa cccatgcaat acggaacgaa tttcattttc ataaccatag ccgtttcgat
    12421 ctgaaaaatc gtgttgcagc cggtcgtatt cttttaaata agaactataa gcaggtgtgt
    12481 ctggcgtacc ttcgctaatg aaaatctcta gctcgcgcat ccgctcttcc atttgtctaa
    12541 cttgttcaaa agctttcagc atctcgttcc aaactgtttc attggaatca agtcctgtgt
    12601 cctgagcgag gtatccgaga gtcgctgttt tatttttagc gatctttcct tcatcaggtg
    12661 cttcaattcc agcaatgatc tttaataaag tcgattttcc agcgccattt ctgccaacta
    12721 aagcgacacg agcgcctgta ctgatttcta aatgtatgtt ttcaaataag acttctgcac
    12781 caaaatggcg agcaatctga ttggcttgta ataatatcat ggaatcctct tttctacttt
    12841 gtgaattctc ctcaaataaa aaactatttt tcaattagta gtctaccata aattccatta
    12901 tactgcgaga tttgcagaaa tgataaataa atgaataaag aataaaattt cacaatatct
    12961 gagcaatgga attgattttt tcctataaat actgctatga tggagaaatc aaagatgtaa
    13021 ttagacaata cgtggaggtc atttcgtgaa agatcatatt attccaaagg cgacagctaa
    13081 aagactgcct ttgtattatc gctatttaag gattttaaat aatgctggaa agaaaaaagt
    13141 gtcttcaact gaattaagtg aagccgttca agtagatagt gcaacgatcc gtcgagattt
    13201 ttcttatttt ggcgaattag gaaaacgtgg gtatggttat gatgtagaag atcttatgca
    13261 cttttttgca aagacattaa acgacgatca attgacaaat gttgcgctga tcggtgtagg
    13321 gaacttggga agtgctttac taaaatacaa attccatcaa agtaatagca tacgtatcag
    13381 ttgtgctttt gatgtgaatg aggaaatcgt tgggcgcatc gtagacggta tccccgttta
    13441 tccaatggaa gatatgtcag aacaaatcaa gatccaacaa atcgaagtag caattttaac
    13501 gatacccgct gaaagagcac aagaagtagt caatcagtta acggaagcag gaatcaaagg
    13561 aatcttgaac tttaccgctg ctcgattgaa tgctccgtca gatgtattga tccaaaatgt
    13621 tgatttgacg aatgaattgc aaaccttgat ctatttcttg cattctgacg caaatgcgca
    13681 ttccgaaaat acagcagaat aaaaataaaa agcgattgaa caaatgaagg caaaactttc
    13741 acttgttcaa tcgctttttt ctgtttggac aacgattatt ttgttcggaa gatttcttca
    13801 taagaaaggg aatcatcttc tgctgtcagt tcatctagta gataacggat ttttttaaag
    13861 tcgatgaacc gtgcttgccg aaagacttct tttccttgat gaaaaatcag gacaactggc
    13921 gcagtcagca cttcaaattt actagctact tctggtgttt caaatgcatc caaatgaagt
    13981 gccggaatat cataatgtgt gagaagttcc tctagtcgtg gacgaacagc gtgacaaaca
    14041 ctgcaattag gcatcgaaag ataaatgacg gcaacggatg actgttgcag tatgtcttga
    14101 acttcagtca agttagtggt ttgtttcatt tttattctcc cttttatgat agaagtgata
    14161 tgtatgagac cttaatttaa tgggaataac agaaaaagtc aattgaagtg ctttggtaaa
    14221 aaataagagt tgtgtaaaaa tttacagagc aacttgtttt ttttgaataa aagcgtatat
    14281 ttgcaaagcg aatgaactag aaataaaaag gaggaaactc atgttttctt ttattccaaa
    14341 ttcggttttg cagatgagta cgattttctt atcgatcgtt attgaagcat tacctttcgt
    14401 attattaggc tgtcttatct caggcgcctt acaagtattt ttgaccccag aacgtgtgaa
    14461 gagatggctt cctgaaaatc gtatcttctc tatttttact ggttcgatct taggattttt
    14521 ctttccttca tgtgaatgcg gcatcgtacc gatcgttcat caatttgtta aaaaaggcgt
    14581 accagttcac acagcgtttg cttttatgct gactgctccg atcatcaatc caatcgtgat
    14641 tttttccact tttatcgcat ttggcaattc ttggcagatg gcaagctggc gggtgatagg
    14701 cagctttttt gttgcccttg tgattggtgt ctggcttgcc tattttcaaa aagacgctgt
    14761 attgaaagaa aaaatcacgt ctaaagtagc agaaaatcac catcatcatg tccatgaaga
    14821 agaacaaagt gagaagaatg aaccaaaaaa tcagctgatc catcaaactg ggcatgtgtt
    14881 gacacattca atcgacgaat tttttgatac aggtcgttat ttgatcattg gcggattaat
    14941 cgcagctgcg atgcagactt atttaccaac tagaattatg ctaacgctcg gctcaacaaa
    15001 gattttagcc attatcatca tgctgttatt ggcctttacg atgtctcttt gctcagaagc
    15061 agatgcgttt ataggatctt ctttgttaag tctgttcggc acagcacctg tagctgcctt
    15121 tttggtcttt ggcccaatgg tcgatatcaa aaatctactg atgatgaaac gatattttac
    15181 aggacgcttt attttatcgc tgatcggact gattgccctt agcgttattg gattcacatt
    15241 atttatttag gagggaaaat atgctgcggt ttttgatttt atctgggtat ttcgaattga
    15301 tgatgtactt gcaagtttct ggcaaactcg atcaatatat caatgtccat tatcgctact
    15361 tagctattct ttcaatgatt ttatctgctc ttttggcttt agttcagctg tatttgtggg
    15421 tcaaaaacgg aaaagcagaa gaaaaggcac acgagcacga tcatgaccat gggttgtcaa
    15481 aaccttatca acgtggaatc gcttatgtac tgctagcttt gccagtaatc gtggggacgt
    15541 tgtttccaac agttagtctc gacactacga tcgtagaagc caaaggattt aatttcccag
    15601 taagtaaaga atcagtaggg gatccggaga tgcagacaca atacttaaag ccggatacca
    15661 gtatttattt caacaaatca gactacttag accaaatgaa tgagttgaaa gaaaagtatg
    15721 gggataagca aactatcaag atcacagatg aaaactattt agaagttatg gagctaattt
    15781 ataattaccc tagcgaattc attggcaaaa aaatcagcta tgaaggattt gtttaccaaa
    15841 caccgaacga tagcaataca gatatcttct tgttccgatt cggcattatc cattgtgtgg
    15901 cagattcagg agtctttggt ttgttgacgc atatgccgga aggagtaacg gttaaaaatg
    15961 atgaatggta taaaatagaa ggaaccatcc aatcagacta ttatcaacca ttcaagcgtg
    16021 aaatcccatc tgtagttgtt acaaacgctg agaaagtctc tgctccgaaa aatcagtatg
    16081 tgtatcgttc tttttaaaag aacaaaataa ataaacatag ctcagacagc tgacacgcaa
    16141 ggacgtcggt tgtctggctt tttttgcatg atgtattcat ttgtttgata tttattttag
    16201 aagcagcatc ctgaaagtat ttgatatcgg tttcaaaggt gttggaaaag attgtgaaga
    16261 gaattacaat ttattcctaa catgtatgtt gaaaagttgt atgaaatgat tttgatagta
    16321 aaatatcgag tggaggtact ttttatggta acgcgtatat ttattttgga tgatagtttg
    16381 gtttttgaaa aaatgctagt agaaattttg gaagaatcca gaattttatt aagtccttgg
    16441 aaatttgaga tcgttcaggg attgaacgtt atacaatttg ttgaattcat taaaaataat
    16501 gaaatctatt cctcagatat ttttttccta gacatcgatt tgaacgccta ttatagcggt
    16561 atcgaactag gcgaaatgct tagagaaaag aatgaaaagt gtaaaattgt ttatctaacg
    16621 tctcatccga ataaagcaat aactgttatt aatcgaaaga ttacgcctag tggttatttg
    16681 ataaaagaat tcacttatga aaaaaataaa aaaagcatcc aaagttactt attgaaagaa
    16741 aaagaaaact atcctgttca acatgctacg aaaaaaaggt ggacaactgt ccgttatggt
    16801 aatagcgatc actatatcca ttacgatgaa atcctttatc taatgagcat gccaggttat
    16861 aaaaataaaa taggcctagt attgaaagat gaagagattc tcgtaaacgg cacaattaaa
    16921 aaatataaaa aatcactcga atatgagtat ttatgtaaag atttaaaagc ttatatcatt
    16981 aatactgaaa acattacatc tattagcaga gtaaacgaaa tagtaacatt tgcaaatgga
    17041 gaacaactag agatgggaaa gagaagtatt gataaactaa agaattttat gcaagaaaag
    17101 atagaaaatg aatcatttta aagaaaacaa agactcatat cttacctgtt agaagagtgt
    17161 tttcctgtag aagagaacaa cccaagtagc agcctagtcg tttctatcct tttttgttat
    17221 gcactctttt tgacatttac tccaccaata acgaagggta caataaaaag taataacaaa
    17281 caaaaggagt acagaaatga ttgaattcat caatgtaagt aaaagttacg ggaaaaaaca
    17341 agcattaaaa gacttgtctc tctcgataag acaaggggaa atttttggat ttttaggaca
    17401 taatggtgcc ggaaaatcaa ctacgattaa aagtttagtt agtattattg aaccatcatc
    17461 gggaacgatt ttagtagatg gaatgaaatt gacggaaaat cgattgagta tcaaacaaaa
    17521 aatcggttat gtgcctgatt ccccagatat ttttttacaa ttgacggctg gagaatattg
    17581 ggacttgatt tcagctgcct atgaattaga cacacaaaaa aaagaaaagc gtttagcaga
    17641 attgacagcg ctttttgata tgtatagtca tcaaaatgaa acaatcgcaa gtttttcaca
    17701 tggaatgcgt caaaaaacga ttttgatcgg cacgttgctg ccagatccag atatttgggt
    17761 attggatgaa cctttgcaag gacttgatcc tcaagcagca tttgatttga aagaaatgat
    17821 gaaggctcat gccgcaaaag gaaaaacagt cattttttct acacatgtat tggataccgc
    17881 acaacagtta tgcgataaac tggctatttt gaagaaaggt gaattgattt atcagggggc
    17941 agttagtgaa ttattgaatg gctcacctga tgaaacgcta gaaaagattt atttgaaaat
    18001 ggctggaagg caagcgacag gagatggacc gtatgtataa gcgacaatta ttagaattaa
    18061 ttcgagttaa tcttttatat gttaatccac agctcacgaa taaagcaaga gagaaaggaa
    18121 aaaagggaaa gcgattaact atctatcttt tgtctcaaaa tcttttttca gggctgattt
    18181 ttttactgat ttacggattt gctatgtttg ctatgaactt tgttaaattg ccaggtttct
    18241 ttacttatta cgtggcactt tttggtatgt taggtttaac tcaaagtata tcagtcattt
    18301 ataatatctt ttttgaagga aatgacctgc aagtattttt gcctctgcct tttggacaag
    18361 aacaggtttt ttcagcaaag attttggtag ttgctttgac catcattcct tttgtttttc
    18421 cgcttctagt catgtttatt ttaaccggat ggcgagctga tctagggatt ttgcttacta
    18481 tcattatctc aatccttttg ttttctctat ttttgattat tcttttttgt ctggctagtt
    18541 tgattgtttt tggtttggct cgaacagcag tgtttaaaaa acatagaaaa acagtttcta
    18601 gtatcttgct tggcagctcg atgctgatcg catttgctgg aatcatgtgg atgaatcatc
    18661 aaacggggac tattgaaaat gagttaacag atcggcatcc gatttcgata ttattgccat
    18721 tctatgatgc tgccagtcag ccactttctc aacaaggttt attgggttgg ggaatactag
    18781 ctatcacggc tctgggattt ttccttgctg taaaacagct gatagtacca aagcttacag
    18841 agcaactgac tgattcaaac agtgagttca aaataaaaag aacccataaa aagaaccaaa
    18901 atgtgcatca gcttttattt agttacaatg ctcaattagt aaaagaacct aatttgatta
    18961 tgcaagttct ttcttcttcc ttgttaactc caatcatttt tattcttgct tttgccctag
    19021 gaggcgaaat aaaattaact gatttagata ataagttaat cggtgttgtt ttcctggtag
    19081 ggatcgcatt ggctttctta acagttaatc agacttcttt tatcagcaat cttatttctt
    19141 tggatcaaga aaattttctg tttatccgtt ctttgccaat ctctatgaac caatatttaa
    19201 aagaaaagtt cagattcgga tggatattgc agagtttttt aactggtggg atcgcattat
    19261 taagcggact gtcttttcag ctgcctttgt tttttatccc tagcttgcta tttggttctt
    19321 tattcggctg ctacttattg tcactacgtt atttcgctag agattatcgg ttgctgctgc
    19381 tgaactggac caatattaac caattattta acagaggttc aggaagtttg ggtttagtcg
    19441 caactatgat gggttcattg atcatcagta ttattcttat cgtcttatac ggaatgttgg
    19501 ctttgtttta tccattttgg ttagtgaata ttcctgtgat cggtttagtt ctaatacttt
    19561 ccgctctatg gatcgtttat tatcaacgaa cgttttggaa aaaatttgaa taaagaagag
    19621 gggccgtgac aaaaatcatg tcgcagcccc ttttttacag atagagggtg ttcaaaagtc
    19681 agtttctcag tattaagtca attttatgta aaaatgtgag aagctatttt tacaaaactt
    19741 tttttaatac tcataattac ttcttattgc tcctgcggtt acttgtcgca atgtttgtga
    19801 gatattttgt catcgctcaa ggctgaacgt ctctttcaca accttttctt tgttacactt
    19861 cgtttattta aaaagccgtt tttttcgttt catcatgatt gcgtcacgct ttactttgaa
    19921 attacggatg ttatggttaa agacttctga taatacaaga cctaaagcga ttgctccagc
    19981 aatcataatt gcttggaccg cataatgagc cgcttctcga taggattcag taacgagatt
    20041 tcgaaccgct tgataagcta aaccaccagg gactagagga accatcccgg gaatattgaa
    20101 aatcgttacg ggcattttca aacgtttaga gaaaagatcg ctgatgaatg ctactcctaa
    20161 agaaccaagc aatgaaccaa aagcagcaga tccgcctatt tgaacacaga tccagtagat
    20221 catccagcca aaagcaccag aaagaccaca acaaatcaat gaacgcctag gtacatttgt
    20281 aataacggca aatgccgcag tcgctaaaaa actaaaagaa aactgaataa atatcgtccc
    20341 catacttgtg ttcctttcct aataaaaaag ctggaaaata aaagcaatcg caaaaccgat
    20401 catcgtagca gtcatcatcg cttctgttcc gcgagaaact cctgagacat aatgtccagc
    20461 tagcaaatca cggatagcgt tcgtaatttg aactccagga actaaaggca tcacacatcc
    20521 gataatgatt agatcctgat tgcttcctaa gccaaaacgt acgctgtaga tagcggctat
    20581 gccaatcaat aaagaagata aaaattccga taaaaatttg attctaaaaa atttcaaact
    20641 atatagataa gctgcgtatc ccaatcctcc aatgactaaa gtcacaggta aatcggtcat
    20701 ctttccgcca aataacacca taattgttcc actgatcact gccgcgctaa cgatttgcaa
    20761 ccagacagga aagaaagaac ggtctcggtc gattgcttgc agttcgcagt aaagttcttc
    20821 taatgttagc tctgaagcaa catatttacg tgaaagatca tttactttta ctactttttc
    20881 taaattaatc gaacggttgg taatttgttc cattcggatg gtcgaggtac gatcaagacc
    20941 aataaagaga ccggtttgag tgacatagct gaccaaacgg aaatttccag aagctaaagc
    21001 aattcgactc atcgtatctt ctacccgata catctccgca ttgctttcca tcatgatttt
    21061 cccagcgagt aaacaagtgt ccagcaacag atctaattct tttcccgtca tagcatcttc
    21121 tccaataatt gattgattag ttttatttta ctacaaaaca taccagaagc gtaaagaata
    21181 aaaaaacttg aggaaggaat aaaagaggcg tatactgttt ttgtattgcg ggtgtagttt
    21241 agtggtaaaa tcccagcttc ccaagctgat gtcgcgggtt cgattcccgt cacccgctta
    21301 aaataggttg tgaaaaagct gctctgcaac aagtaataag aacagccaag caaaaacaac
    21361 ttcttatgtt tttaacttgg atggacttat taccgcagat gcaagctttt gcgcaccgtt
    21421 taccaagcgg ttgtgaaatc agtgtattgt cttgagtatg aagaaaattt ttgaataata
    21481 ataaatacct tacagataga gtggtgactg attagagacg aggaatccgt ctcttttttg
    21541 tatttttaag ccatcttaaa acaggtttta ttttttgctg tttctatttc tttctagtat
    21601 ttatttatag gggaacatat gttaagattt agggagctgt tagtaaagga gtaaagaaat
    21661 gagcaaaaaa acaaccaagg cgatcgtggg gatcgtcgct gctgtgattg caggggcatt
    21721 cggtgtttcc accacacaaa aagattcaga tatctttcag cagattacaa ctatttttga
    21781 ttccacacca caagcttctc aagtagcgga tgtgccagaa tatgatggaa aacatcaaga
    21841 aatagaaatc aatgggaatc agccggaatt tacaaaagag gatcttagcc tttccaaagg
    21901 aacatgggaa agctattcaa atattgataa attgaacaga gtaggacaag ccaatgctat
    21961 gcttggaaaa gatttgttcc ctactgaaaa gagagagcct ttgtatattg acccgacagg
    22021 ttggaaacaa aagaaattaa gtgacgggca atggttatat aatcgcagcc atttaattgg
    22081 tttccagctg actggtcaaa ataacaacat taaaaacttg atgacaggga cacgttcgtt
    22141 gaatgctcca tacatgcttg ctcacgaaaa tgatattgcg gcatatatca aagaaacaaa
    22201 tcatcatgta cgctaccgtg tcactcctca ttttgagggg aatgaactgg tagccagggg
    22261 agtgcaattg gaagcagaaa gcattgaaga caaaaaaatt gaattcaatg tctttatcta
    22321 caatgttcaa gatggctaca cgatcaatta tgagacagga caagcaacaa aaacaaaaaa
    22381 ttagtagcag ctattcgaat tttttatgaa tattatgaaa aaattgtgta aaaacataca
    22441 ttcaaagaaa tatagcgtat actttatgaa aagagtactt ttgataaagc tacagaaaaa
    22501 aggaaaaaaa catacaaata gtgtttctta atttattcgg aagtttggaa tatttgtttg
    22561 tagttttccc tttcttcgag taaaataagg ttgtagaagg taagctggaa ttaccttgcg
    22621 ggaaagaggt gtagaatcat gggttttaca gatgaaaccg tacgttttgg ttttgacgat
    22681 agtcgcaaaa aagaagtgag cgaaacatta gcgattgttt atcgggcttt ggaagaaaaa
    22741 ggctacaatc caatcaatca gattgtcggc tacttgcttt ctggagaccc gtcctacatt
    22801 cctcgttatc gtgatgctcg aaatttgatt cgccgtcacg aacgcgatga gatcatggaa
    22861 gtaattgtca aggactacct ctcaaatcat ggagtgaatt tatgagagta atgggcttgg
    22921 atgtcggttc taagacagta ggtgttgcta tcagtgaccc ctttggctgg acagctcaag
    22981 gagtagagat cattcggatc aatgaagcag aagaagagtt tggctttgat cggttgaagg
    23041 aattagtagc acaatatgaa gtagaccggt ttgtagttgg attacctaaa aatatgaaca
    23101 attccatcgg accaagggca gaagcttcca tgtcatacgg agaaaagatc aaagaactat
    23161 tctccttgcc agttgattat caagatgaac gattaaccac tgttcaggca gaacgtatgc
    23221 tagtagagca ggcaaatact tcaagagcca aacgaaaaaa agtcatcgat aaattagcag
    23281 ctgtgatgat cttacaaaat tatttagacg gctcaggtaa gcaattattt taataataaa
    23341 agaaaagagg taatcttatg acagaacata accattccca cgaccatgaa ggacacgaac
    23401 acatcacatt ggtggatgac caaggaaatg aaacattata tgaaatccta ctaacagttg
    23461 acggacaaga agaatttgga cgtaactatg ttcttttata tcctgctggt gtttcagaag
    23521 atgaagatgt agaattacaa gcttatgcat acatcgaaaa cgaagatgga acagaaggcg
    23581 agttagaaca aatcgaaaca gacgcagaat gggacatgat tgaagaagta ttcaatacat
    23641 tcatggcgga agaagaagaa tagactataa agctaggaga aaccttgata tgatagggtt
    23701 tctccttttt ttatttttta ctgtatgcca tattgtatgc catctagtca atatttaagt
    23761 gttgtactaa tttttcaaca gtcttttctt tcgtttcttt tgccaagtga ccgtaacaat
    23821 cgatcgtctg actaattttt gtatgccgta atctagtttg aacttctacc atagtagctc
    23881 cagcgtccaa gagcattgaa gcatgagtgt gtcttgctac atgcacaccg agaaatggaa
    23941 ccatagcttt ttcacaaata gtaatcaatc ttgcataaat agcgtggtgt gtgattagtt
    24001 ctttatagat accacaaaaa atcatagtcg gatcagttac accaagtgtc agcatatatt
    24061 tccgctgatt aatctgccat ttttttaata tagaaattgt tctttcatct agtggaagaa
    24121 ccccagcact acaatcagtt ttaggatcac tgattttata gccatttttg gattggctta
    24181 gtgttttatt gatggtgacc gtattttctt gaaaatcaat gtctgaccaa ttcaatgcaa
    24241 gggcttcact gatcctaacc ccgctataaa agagaaagcg tagtaacgtt ttatcaaagt
    24301 cgtttcgata agtttcaggt ttgctgttta ggtaatcaaa caaggtgatc agttgttctt
    24361 tactgtaaac tttgaccttt tctttatgct tagtggtggt tcttttaggc attaatacgt
    24421 tgtctagtgg attagtctca ataatcccaa gattgatagc gtagctaaaa atacgcttag
    24481 aagttgaatg aagtaccttg tattggtcag tagtttcagc ccagctattt acaatttgtt
    24541 gggctttttt taccgtgact tcattcacac gaagatttcc atattttgga agaatccact
    24601 ttctaaactt gatttcagtt ttcatgaagg tggcttctcg gacagtggtt ttatatgttt
    24661 ctagccacaa gtcataaact tctttaaacg tttcgttgtt cgctttttta ataccgtttt
    24721 tatcgtagtc cacttgcaag cgtgacaagg caagctgggc ttcttttttt gttttgtaac
    24781 ctcgtctacg aaccttatca ggtttgccag tcattttgtt cattccaaga taggcttcat
    24841 agagccaaaa tttacctttt ttagtttcat attgcttgaa tgttgccata atagttttcc
    24901 ctccatagtg tagcaagccg tgggggcggt tggaggaggc agtttattat tattttgaat
    24961 agtgtttatt tagctcagta acaagagctg tgaactcttt tagaagatca ttttcataaa
    25021 attcttgata ctcttttttc tcatctgtat ttttttctga atcattaatt aatgagtcat
    25081 atcgtaaaag cgtatttaga agagctgcga gataagcaat tggggtactc ccgtcaggaa
    25141 attcaattcg agatcctgtt ttaagatatt ttattgcgtt tgttaaataa atcttctgtg
    25201 agacagtcag gttatcttct tcgatgttat tgatagcatt agaaagatta gaatcaatta
    25261 tagagttatt tttttcaata gtttttttca attgcttttc aagcacattt cgcttaattt
    25321 cgggcatcat atgcatatct cttaatgtat aagatccgtt taatagatag gtagtggata
    25381 catctaatac ctttgaaagt tgtttaagcc tggtgttatt gggaagatta tagccatttt
    25441 cccaattact aacggcacct ttactggccg gtggggataa tttacctcca agttgttcca
    25501 ttgagagtcc cttttcaatt ctagccagtc taattctttg tcctgtctcc actgcttcgg
    25561 ggtatttttt tctagccaca ttttcaccac cattcatata ttttctctac tatatcacga
    25621 tttaaatatg tgtgaaacat aaaagttcaa aaaagtatgt taaaattctt gcaaatgaaa
    25681 aggtatgatg gtatattgag tttataagag gtatagaaat gtattcaaga gcctcttgca
    25741 tttttttaaa atctttgtat agaaatgtat gctaacgagg ggggataaat atggaattaa
    25801 aaattgacaa actcacgctt gatgatccag cttttaaaat tattgacgat cgagttgttc
    25861 aacaagtaga gcaaactcaa ggaacgttac tttcattatt agaaaaaaag taccagtttc
    25921 caaaatatat gaacaaggca caagctgcta agtatatgaa cgtttcatac aatactatga
    25981 tgcagaaata tgttcctaac ggtttaaagt tgattatagt tgatggagtc gtaagaattt
    26041 cacaagatga atgtgatcgc tttatggaag aataccaaaa ataaacgcaa gccgtggggg
    26101 caaagggatt aataggagga aataataatg gaatcgacac aaaataaagc aatcgaaaaa
    26161 gtattatcaa caattcttac agaagacaca gcggcagaat tagcaaactt ggaaggcaaa
    26221 gcgctagaag aaacgtttga atgtttgtac gaacaaatgg actatcaaaa attgctacca
    26281 caagaaccaa cggtaagcgg agtattgcgt ggcttgaacg atttagtaca agcagagttt
    26341 aaagaacgac tttctattga agagtatcaa gaaattttgt atcagcaagt tgagttgtta
    26401 gcgagcttgc tagggcttga actggaggac gttgaatgaa aactgaaatt tggaacggac
    26461 acattataag atttgtcgat attaatgatg aatggtgggc agtggcgaaa gatgttgcgg
    26521 aagcgttagg tcttaaacag gttactagag ccattcactc tttgcctaaa gatggggtta
    26581 ctactagtaa ggtcatcgat tcattgggca gaacacaaga tgtaaacatc atcaacgaaa
    26641 aaaaatattt accgcatggc attcaaaagc cgtaaaaaag aagccgaggc atttcaagac
    26701 tggattttcg acatcatcaa agaactacgc cagtccacag gacttgaagg ctttcaagta
    26761 ttccgcatgc tagacaaaga acaccaaaaa gaagcaatga ctaaattaag ccacgctatt
    26821 actgaaccaa aaccagtaga ctacattaaa gcgaatgtga tagccaataa agctgtatca
    26881 actatttatg gtcattccaa aatggtaaaa aagaaagata tgactcctga aatgttggtt
    26941 gatcgtgaac ctattttaga tgagacagta gaattaatga ctgttaaaga aaaatatggg
    27001 ttgcagtttt cagtgagtga gaaaatttat aatcgttctg ctgaattgca gacaacttag
    27061 gagcattaac atgatcgaga acaaagaaaa agccttatcc ataacagtct tggcggacag
    27121 gataaaggca acttaattgg gataaaaccc gatattcttt gttccgatta taccatatta
    27181 aaaaatcgga gggaaccatt atgaaacgaa acttaattat aggaagcatt ttaatcggcg
    27241 gaattttcgg cttattacca ttaattgcaa aaatagctat ctttgcagga ttagcgttat
    27301 atctcttcac tgcttatgac gagtacgact atcaattaag aactggaggg cataagtaga
    27361 tgtacaaaac aaaactatta aaccaactcg atagccttga attagaggaa attaatcaag
    27421 ggatcgctga actagaaaat aatatcggga aaacctactt tggaaattca ttcaatgaaa
    27481 aactaacagt tttgtatgta ctcaaaaaac atgcagaaca caaaataatt tgtcgagaaa
    27541 tcaacgagct aaaaaatcag attttaaccg catggttaaa cattactgat atgcaagaag
    27601 caagggtgaa aacatttaat acatgggtaa agtatcagaa tcaattaaaa ggtgctgaat
    27661 ttgttcggga tgggttgaaa tacgaacttg agcagttgaa gctcatggag gtgtctgaat
    27721 gatttatacc agtattttga aaaaggagga gaggtttatt ggcaaataat tatcaagcag
    27781 caacggaatt acaaaagtcg ggatggtcaa tttatccctt atctcctgga actaaaattc
    27841 caataaaagg ctctagtggt tggaatgatg caactaacga ttctaatcaa attaatgaat
    27901 ggtggacgga agatgcaact agaaatattg ggttaatgct tgaagatcat caaatggtgg
    27961 tagttgacct agatcaacat tctgaaacac gaaacggcgt tgagaattat aaaaaacttg
    28021 cttcaatgta tgatccattt ccttctactt atacggaaat cacaccaggt aaaggaatcc
    28081 atttcttctt taaaattcct gatggtgtaa ttatccaaca agaaacaggt gcatttgctg
    28141 atatcttcgg gcgtgatgat aaaggtaaaa gtttaagcgg gattgatatt atcactaaag
    28201 gagttccaat agcgccaact atgattattt caagaagtgt tgggagagca tatgaaccta
    28261 ttgaaaacgt atctctaaat aatattgcac cagctcccga ttggctagtg aacctactgc
    28321 taaaaaaacc gcaagaaata aagactcaat accgacccgt agcaaaaaca gggacagcaa
    28381 aaaaattaga tatgattgtt caaggcgctg gcgaaggtag tagaaacgat catttaacta
    28441 agttatgcgg ttggttacta tggcacggtg tagataatca aactttagca gaattgattt
    28501 atacagcaaa tgcgtacaat gaaccaccgc taaaagataa agaagtgaat caaattattc
    28561 gatcgatgat taaaaaagat ttgaggggga aagctcatgg ctcataagca taaattagat
    28621 agtattttag actttcccga agccagcgag cgtgaagaca atatcattga attgaaaacc
    28681 tggatgtcac gattaagatg caataaagac gatcagatta aaagtaacag tgttgtgaat
    28741 gcagaattga ttttaaccaa tgatagcaat ttagctggaa caattgctta taacgaattt
    28801 agcggataca ttcacctatt aaaagattct ccttggatca atcgtaccgc tggagaatgg
    28861 gaagacagtt ttgaggatgc cttaacggca tatattgaag aaaactacaa tgtagttttt
    28921 gatgacaaca aaatacacaa agcagtagta aatgtggcta gaaagaatgt tttcaaccca
    28981 gtaaaagaac gtatcgaaaa agttaaatgg gatcaacagc ctagactgga aacactgttt
    29041 attgaccttt taggggtaga cgacaacctt tacacacgag aagtaacaaa acgttggata
    29101 gtcggatcag tcgcacgtat ctataaacca ggaataaagt ttgagatcgt tccagttttg
    29161 gatggaccgc aagggattgg taagagtacc gttccagcat tactttacac ggatgatttt
    29221 ttcaccgatt cattagattc gctaggtgaa aagaaagatg attatatgca gcttcaaggc
    29281 aatgtaatta ttgaacttgg agaattatct tcaatgaaca aaactaaaat tgaacagata
    29341 aaaaacttca tcagtgccaa gatcgataaa attagacctc cttatggtcg gaatgtcata
    29401 gcttgggcaa ggcaatgtgt atttattgga acgtcgaatg atgggcagta tttgaaagat
    29461 gatacaggaa accgccgttt ttatcctttg ccttgtaaga ataagccaaa gatgaatccg
    29521 tttaaaacaa aggatgaata ttttcttcaa gtgttagctg aagcaaaggt gctttttgat
    29581 aaaggacaac gaatctattt tagtcccgat gaagataaag aagtcttaga aattgcaaaa
    29641 gactatcaag aggatgccaa gatggagaat ccaattaaag aagcaatcac taaatatttg
    29701 gaaatggaaa tcccgtataa atgggaagaa gctccagcgt gggcgagacg ttcttattat
    29761 cagaactatc ctgacagtaa atgtaatgag aaaaatttac agcatttcta taatggagct
    29821 actatgcaac aagagtttta tttagttgat agcgtactca ccgctgatat tttggaagct
    29881 gtttttgata aacaagcaaa ggatttattg aatggtcgat cagatgcaga aactaaaaag
    29941 attgccttga ttattagcag tattcctggt tgggaacgga agcaactgcc aaaaagaaat
    30001 aaacgacgag gattttacaa taagtcaaat gcctatgaaa ataaaaatcg agctggaaag
    30061 tacaaaaagt aacgggtagt aacgggtcag taacacctag cccgttacta gtttaccctt
    30121 actgccccaa gggatacagc aatttggtaa cggggtaaca ccttatttcc ttaaaacttt
    30181 tgaaatagtt agctaatatg ctggggaata atgttactgg attttacccg ttacctgtta
    30241 ccaaaacgct agaacccttg tccccgttgg gattgagtgg taacgggtac ctgttacgta
    30301 ggtgttacca cgtgttacga atcaaggagg aaagtaaatg ctcaatatta tttcaacaaa
    30361 caaagcaccg aactttcaat atacagatga aatggatcgg tttctaatga acacgctggc
    30421 atttagtgtg ggattggtaa cagaagatta ttccacattt gatccagaag tattaaagat
    30481 aatggaagaa gaacctgatt ggctgcagga gtcagtagcg tggtgtcaat cgctagtagt
    30541 gggatcattg gtagatagtg gcaactatga tgatacaggt gagctaatgg atgaatttaa
    30601 ttgtctgctt aacctatatg atcgagcgag acaacgagag cttacatcga atgaggataa
    30661 tttgttttta aacatccatg ataagttctt ggctttgcta ctaacagatg atgaattgat
    30721 aactaattta ttggaggtag aataattatg catatggaaa gtattagata cttcgatggt
    30781 gagaaacaca ttgagtattc aaacactcaa gaagatgtag actttattag ttttgaggaa
    30841 gataaaatca ctaaagttaa ttttaaagac ggaacgtgct tgaagatagt ttcgccttat
    30901 attgaataca aatcaaaatg ggaacaagat gattctgacg aacgagatcg ctttggttgg
    30961 taatctatgg cagttaaaaa acaatgtaat catgctggat gtaaaacatt gattgattat
    31021 aggcagaagt attgtgggaa gcataaagca aagcaaacgg caattaagcg tgaagaaaga
    31081 aagcaaagcg aaggtaaata ttttcaattc tatcaaagta gaacatggcg aaaggcttca
    31141 tacttgtatc ggttaaatca tccagtatgt gaggattgcc tagaagaagg cttgataagg
    31201 aaagctgatg ttgtagatca caaaattgaa ctaaaagatg attggaatag acggttagat
    31261 gaaactaatt ttcgttcttt atgccatgca caccataatt ccaagacagc caatgaaaga
    31321 caaagacgag aaaagagtac cctttgagtt aagggggcat agtgtgaaac ttactgacaa
    31381 tcgatgccta ctcattttgg tacaaataac cgttgtgaaa aggcataagg gtaattacaa
    31441 ataacgatta cacttgtaat cacaagtgaa aaagtatata atgaaagtag gagatttatt
    31501 gaaaaataca attgttatta ctgataaaac agggaaacag cgcaccattg agttacctca
    31561 ttttggtcaa gtgaccattc aaatgcagaa cggaaaaatc atttatatag ataaattaga
    31621 taaagaaaaa ttctgatcga aaaacgaagg aatgtgagct taattgctta cgttcctttt
    31681 tcttttgtct gaaaggagga caacatggga caccctaaaa tattacacga tacaaaaggc
    31741 aatatatcaa gtgaagaaaa agcaatccgt gtggacgcta gagaagaatt gttcaagcag
    31801 caaccattaa taaatatcac gccccctgac tggatggcag cgagcgctag aagtgaatgg
    31861 aatcgtatcg taccaacatt aaaaaaagat tatccattga gtgaagcaga ttatggttca
    31921 ttggtagcct attgtttagc ctttgctcga atgaaaacag ccgaagccga gataagaaaa
    31981 tcaggtacgt ttatcacatg cgaaaacgga gtaaagaaag ccaatccagc ggttcgggtt
    32041 caatcacaag ctatgagtga tttgaaaaaa caagccacct cactaggtat gaccttagaa
    32101 tcacgatcaa aactagcttt gaacaaggtt aaaaatgatg aacccgaaga cccgttcaaa
    32161 gagttgatga attcatgaat gattacattg agaaagtcct atcaggtaag ctgattgcac
    32221 ccaagaaaat tatccaagcg tgtgagcgtc atataagcga tttggagcgt tctaagtcag
    32281 atagcttccc ttatgtgttc gatgaagaac aagccaccaa agcgattaaa ttcattgagt
    32341 tgctaccatc tacggacggt aaagcaatca agatgttagg atttcaaaaa ttcattctag
    32401 gcagtcttta tggctggcgt actaaagaag ggaattacag gcgattcaat cgagcgttta
    32461 ccagtatgag ccgtaagaat gggaaaacgt atatcgcaag tggcatggct gccaatgcgt
    32521 tgattatgga acaagaacca gcagaagcaa ggcaagtatt gtttgtaagt aacgctttga
    32581 aacaagctaa attgggctat gatatgctgt ctaattcact tagaaacgtg gtcaagtcta
    32641 gtaagttttt aagaccacaa ctgaaaatta tgaactctaa gattcagcac ttgccctcta
    32701 attcgttcgc tatggcactg gctagtgaaa ccagcacgct agatgggttt gcaccaacaa
    32761 ccgcaattct tgacgagtgg cacgaagcaa aaactcgtaa aacgtacaac gtcattaagt
    32821 caggaatgac ccaacaaaag aacggcttat tgtgtgttat tagtaccgct gggcttgatt
    32881 taaacgttcc tatgtacgag gaatacttat tgttagatcg tgtgctaaaa ggcgaagaac
    32941 aagccgacag gtactttata gcgatatggg aattggacga tcccgaagaa attcacgatc
    33001 aagagaaatg gatcaaagcc aatccgattt ttgaaagtga agaaatcaaa aaagtaatga
    33061 ttccaaccat tcaagatgat gtgaaccttg ctttgaaaca aaataacctt aattctgtat
    33121 tggtgaaaaa cttcaattta tggagacaag cgagtgagga cagctatatg attgctgaag
    33181 actggcaggc aaccgaagta gaaccacaag atattacagg caagcccgtt tatatcggag
    33241 tggatttatc taaaacagat gatttaacta gcgtttcatg gatcgtaccg ctagataacg
    33301 gcgaacttta ttgtgattca catagctttg tagccaccaa atatgggctt caagacaaag
    33361 aaaagcgcga cggtttgcct tatcgagaac tagaaaaagc tggtgagtgt tccattactc
    33421 aattagaaag tggaatcgtg gactatgacc aagtatttca gtttattcaa gatttgattc
    33481 aagaaaatga tttggaatgt atggggattt gttatgaccc gtataacgct aattcgctta
    33541 tcagtaaagc tgaaaaagcc aactacccaa tgttagaagt aagacaagga acgattactc
    33601 taaacgttcc gacccgaact tttagagaac aagtttatga aggcaacgtt attcacaata
    33661 aaaatacgat tctcacccat gcagtgaaca acgctatttt aaaaacggat aacaacggca
    33721 ttcagattaa taaatcaaag aacagtaaca aaattgatcc aatagctgca ttaatcaatg
    33781 cctatgtgtt tgcaatggat tacttcacca caacggaagg agcgaaagca gacaatgaat
    33841 tttatacaag tgaagaattt tctttctaat tacattcata ccgttctttt acttcttgga
    33901 ttggtgtgtg tgttggttgc tatcacctta ctaacaaatg tctactatgg cttgttagcg
    33961 ctgggcatag tgcttattgg ggtagcggtc atgctaaata cagaacagaa gggaggttaa
    34021 aagatggcat tttttaaagc gagacaaaat acaacgggag atcctttcct acaacatgtg
    34081 gtatcaatcc aatcggatga ttacaccacc agctttacaa gcgttcgtgc attaagaaat
    34141 agtgatgtgt ttgcagccgt tcgaattatt gctagtgata ttgcttcaag tccgattcaa
    34201 ttggtcaaaa acaatatgcc acaagctgat aatgaactgg tgaagttact aaacgagaaa
    34261 cctaattcag aaatggatgg ttggcattta aagtttgctt tggcggtcaa tatgttgcta
    34321 aacggtaata gctttgcaga gattaaacgc aacggtgaaa aggtagaaga aattcactta
    34381 ttacctaact caagtgtaac ggttactcaa ttagataatg gtacgttgtc ttatcagatt
    34441 ggtgataaaa aaagacgtgt gaagtctagc gatattttgc acttcaaata tttcacccaa
    34501 gatggtttaa caggattacc accactttat gcgttacgtg atgaactgaa aattcaacaa
    34561 gctggcaacc gcacattgca caatttcttt actcgtggtg tcagtgggtc aggtattttg
    34621 aaggttcata agtcagattt agacggatca gcaaagaacg caattcgtga aaagtttgaa
    34681 gaagccaacg gttcaagtag tggagataat gctcttagaa cgatcattct tgatgaaaca
    34741 atggactaca aaacattaga aataaacacc gatgttttga agcttatcaa ttccaatgat
    34801 tggaacacaa aacaaattgc caaggcgttt ggtgtaccga ttgagcgttt aggcgttgaa
    34861 aatgaacatt ctagcaccgt tcaaagtaat ctccagtata tccaaagtac gctgattcat
    34921 tactttaatg tgtttgtgag tgaattagat acgaaacttg aaaccaatat ccgctttaat
    34981 tccgatcagt tactagagac tgatcctgaa acaaaagtaa aaaatgtatt ggatcaggtc
    35041 aaagggtcac ttctcacgat taatgagggg cggtcgaaaa tggggctacc ccccatggat
    35101 ggtggcgatc gtttactagc aagtttgaac tttacgtatt tagatacgtt ggagaaatat
    35161 caattaaaag aacaggaagg agttacacca gttgaataat gaagaagaaa aggaaaaacg
    35221 gctgacagaa gaagctgagc taacagccga ttctcccaaa gtagagaaag aaaatgaaaa
    35281 acaaccaaca gacggcaaaa ctatttcagg ctatgcgttg aaattcgggc aaccgtcaaa
    35341 agatttaggc ggctttgtag aagtgattac acccgaagca ttaaaagagg tggatttatc
    35401 aaatgtgttc ttattatatg gacacgatta ttcaaagcca ctagctagtg tcaaagcagg
    35461 tacgttgaaa ttgaatgtcg atgatacagg attacatttt gaagcagagc taaccgacac
    35521 gacctattca aatgatgtgt atgagaatat ctcaaaaggg gttgttgatt ctatgtcctt
    35581 tgggttcgtg cttggattag attcattcga caaaaaagaa gatggcacaa ttgaacgatc
    35641 aatagataaa atcaaagcac ttaatgaaat tagtgtcgtg accgttcccg cttatgattc
    35701 atcaaatgtc caagtaaata agcgttctta cgaatcgttt atgagtaaca accaagcaaa
    35761 acaaacaaac aatagcttag aatccacttc taaagcacaa aaggagagta ataacatgga
    35821 aaaaacttta atcgataacg aaaaaacaga attgcgtggg tatgaagaat atatccgttc
    35881 acaaggcgaa gtgcgtgatg gagtcactac tgtaaatgca gcggcagttg ttcccgaaga
    35941 agtaatcggt gaagtctttg atttgaaacg ttcaaattat aacttagctc aatatgcaac
    36001 agtaaaaaca gtatcaaatg gacaaggtaa atatccagta gcaactaacc aacaagcagt
    36061 gttagcaaca aaagctgaac ttgctgaaat tggtgatatt gacgctgaaa tgtttacttc
    36121 agttgattat aaagtagaaa ctcgtgctgg taagattgcc ttatcaaatg aggttgtgga
    36181 agattcagca gtgaatattg tacaagaggt caaagatcag ttagcgaaat tggtagaaaa
    36241 cactgacaat aagcatatca tggatttatt aaaaacattc actaagaaaa cggctgctac
    36301 gttggacgat ttgaaacaac tatacaatgt ggcattagac ccagcattaa ataaaatggt
    36361 aattctaaac caaagcggat attaccacct agatacattg aaagattcag atggacgtta
    36421 cattttacaa cccgatgtga cagcacctag tggtaaatca ttattcggta tgccagtagt
    36481 attgattgca gatacattgt ttgccaatcc taaagctgga acattcccta tgattatggg
    36541 ggatattgca caatctatct ttgttgctcg tagaaatcaa gtaacgactc aatgggaaaa
    36601 attcgattac tactcacaag gacttgcagt gatcgttcgc aacgattata agaaaattga
    36661 tcaaaatgct tcagtgtata ttgagtttac gccagttgta acaccaaaag tatagaaaaa
    36721 tattgggcgg cggtttatcc cactgccttt ttttcattag gagagataaa atggtaattt
    36781 tggatagcat aaaaaaaagt atgcggatcg atcacactat tgatgatgac tttattcaac
    36841 aattgattga tacagcaggc gaatatataa aaagtgctat tgatagtagc gcaacggata
    36901 aagatatgga taattatcag caatttgatt tggcggtgtc attacttact caacattggt
    36961 atttgaaccg tcaagaagcc agcagcgaac ggataccagt aacggtacaa gcattagtac
    37021 aacaaatgag aggtgcttat tatgccaatc attaagaatg taaatgaatt gactgagaga
    37081 gtcaagttta aaaagacaaa acgggtaaaa gatgaagatg ggcagatggt ggatagtgaa
    37141 gaaactgtat ttgaatgttg ggctaatgtg cgttcacaaa tgctaaaaga cgtgcttgct
    37201 agtgtaggta ccattcttga aggaacgttg acgtttatta ttcgatacga tcaagattat
    37261 gaactaacaa acgatatgaa agtagcttgg aaaaacaaga gttataaaat tatttcaatc
    37321 aatgaaggaa cggcgtttaa agattataca acgatcatag ctaaacagat ttcttagaat
    37381 gattacactt gtaaacgttg tgggtattta gtataattaa ggtagtaaat gaagggttag
    37441 ctgcctgaat tttgcaagac ttagctagtc gaaatctatt gttggactga aaattgtagg
    37501 tgtggttgca aacatgctgg actaagaaag atgatagatg atgaacgttc aattttaata
    37561 caggcatgct aggactgatt ggatggtttt ggcaagctga ttgtgtagaa atcaaagaac
    37621 acgtctgttg tggacgtgtt ttttttgtta taattcaaaa gaggtgattt aatggacaaa
    37681 gcagagttta caaattttta ttggaagtat tatctacatt tagaaaatga gtttattaat
    37741 accacagatt ttgtgatgct cgatgagaat aatttcaaaa cattctctat tgaatatcaa
    37801 aaactactac tagcgattgg ttcggagtgt gagattattt tcaaagaact ttgcggattt
    37861 gatagcagta gcaataaaag cataactgat tttaaagctg ttattaaatc tagcgaatta
    37921 atgtgtttgg ataatggtgt tagggttatg aattctttaa cattgacttc actaaaacct
    37981 tttggagatg ggtggcctga agatacacca gattggtgga aaatatataa cagagtaaaa
    38041 catggtcgtt cttcaaatta taaggaagca aatttggaaa atgtgttgta tgctttagca
    38101 agtctatatt tattagagga atatttgcat aagaaagtaa tactagaagg tgaagtagac
    38161 tttataagtc cagagtcaaa tctatttacg ttatcttggg aaagaaaaca ttcgtcgatg
    38221 caaaaagtga cgtttgaaaa agtggattca aattatgtag ctccactcat tgattttaaa
    38281 gaacaagaag aaatttaaaa acccccacgg cttattgaag tttttgtatg ccatattgta
    38341 tgccatttgg tttaaatcgc ttgaaaaata gtgatagata actctaattc atttagtcat
    38401 aaacgctgtt ttgataacct ttatagttct ttgatatggg tttctcattc atggcggaag
    38461 aagaagaata gaacaaaaga atgaattaat aagatataaa aaaagggatt gtgacaaacg
    38521 tcttgtcaca atcccttttt ccaaataaac gatgttcaat cagctgcccc tcggcattcg
    38581 ctcgaaaaac ggcgaaatat gaagaactat tttgccattt gtttctctaa taatcaggtc
    38641 aaaacgttga cttcacagtc tcttccttaa acggtgttct cctgccaact cctcggtcac
    38701 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    38761 agctgaacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
    38821 taataagttc aaccaaacga aaattatgag gagctatttt tgtttggctg ttcttattac
    38881 ttgatgcagg acagcttttt cacaacctct tttcgtttag cactaaattt ccttaaaaag
    38941 tagaaaagct ttcgaagcct cgtttgtttc ttttactgta taataaaaga aaagagtaac
    39001 aatcgctaaa agatataaac aagagaaagg aaatacaaga tgattggtct gatttatttt
    39061 gtggtgatcg ttctggcgaa cacagttggc gctatttccg ggatgggtgg aggcgtgttg
    39121 atcaaaccga ttcttgatct tatcggcgcc cattctgttg cagggatttc tttttactca
    39181 acagttgcgg tattcacgat gtccattgtt tcgactgtgc gacaagtatc aagcgggaaa
    39241 tcactgaatt ggcagattgt cggttgggta tctagtggag ctgttgttgg aggagtagtc
    39301 ggcaacattg tattcgaagt ttttttacag ttatttgaaa acgagaagca tgtgcagctg
    39361 atccaaattt ttttgacggt attgaccttg gtttttgcgt tcttctacac caagcatcat
    39421 cagccaaaat ttcatttgac gtcatggact tggtacctta tatgtggggg ggttctgggc
    39481 tttttagcta gttttttagg tattggtggt ggaccagtca atgtttcttt gttgatgctg
    39541 atgtttgcat tgccaataaa agaagcaaca ctatattctc tatctacgat tttcttttct
    39601 caactggcaa aattggtaac gattgcttta acaagtagct ttatgcgctt tgatttatct
    39661 atgttgtttt atgttatccc cgctgctgtt attggaggtt tgtggggagc aagatttagc
    39721 agggttttgt ctccaaaaaa agtgaccttt atttttcaag taattgtcct cgtggttctt
    39781 ttgatcaatc tttacaatgc tttcgtcatc ttacaaacat taggagaatc tagactaacg
    39841 caagtcaaaa tcactgaaac atattgttct aaatgaaaaa aggagtatac tgtgataaaa
    39901 aaattcattt aaaaaaataa aaattggctg gtgatttgga tgttgcggga aatgcgttta
    39961 ggagatgcag aagcactacg agaaatcaat gcggaacaat taggatatga tgtaccatta
    40021 accgttacgg tgagacaaat gaagaaactg ttgaaggagc cagaaaaaaa ttttttcttg
    40081 gtctatgaag atcctacttc taaaaaagca gctggctatg tacaagcgga gtcctatgaa
    40141 tcgattttca gcgatccgat gttcaatatt atggcacttg ctgtgtctaa acatgctgaa
    40201 aaaagaggaa tcggaaaggc cttgatgact ggcgtagaag aagcgaaaag acgaggaata
    40261 tcagcaataa gacttaattc tgcagaatac agagtagaag cacaccgatt ttatgaacgc
    40321 atcggttatc attccgataa aatgcaaaaa agatttttga aaatcctgta aatttttatc
    40381 aaaaaatacc tggtcatcaa gccgatactt gatgatcagg tatttttttt gactaagaaa
    40441 gtaattgcat caagaaacct gagatgatca aaacgatggc accaccaagc cgattgccca
    40501 tttgagcaaa agcaataagt tccatccgat tagctgcaga taagacagct acatttcctg
    40561 taccacccat cgagttgttg cataagccag cagtaatagt tgattcgatt ggatataaac
    40621 caaacaaacg accgacaaat cctgaaacta ctgaaatcgc gataacactt gtcaaacata
    40681 agacaacaaa ttgccatgtc aaagcagaag ccaatacatt tagattcaat aacgcaatac
    40741 cgataccggc aagaacagct gctgtcaagt ttttgacaat caagttgttg aacatgatag
    40801 ctgcatcttc gtaatattct ggtaataggt ttgcgatttt acagataaca acgataatga
    40861 tcatgaatgc gtaagcatgg actttaggaa caaaatagtt acagatttgt cctaagatga
    40921 agaaagtcaa tgataccatc aaaccgacac ctaattgtgg aatggaaggt tttacatttc
    40981 taggttttcc atcgccaagg tcgccttttt ccattaattt accattaccg ttgaattttg
    41041 gcattgattg tcccgcacga gcaactaacg ctgcaccaat gatagctagg acatttgtca
    41101 ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc gtccgtacct aaaacatttg
    41161 cataaatcgt tgataaaggt acagctccgg ctccaacacc accagccatt gcgggcaatg
    41221 agatatataa aacggaattt gcaaaaccat ttccgataag catacctacg agtcctacgg
    41281 caaaaaagga gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca
    41341 tcaatagatt acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt
    41401 ctaaaaagcc catattgctg acaaagtttt ctgtggattt tactacatac tcaggtaaga
    41461 tcccaaatgt agcaatcgcg gcagaggcaa agatcgcaaa aacggagcca ccacctaaat
    41521 aacttttgac gatagggatt tttgttccga taaaatgaaa taaattaccg aaaatgacca
    41581 aaacagcgat tgggccaatc atattcgtcg gtaattttcc taaagccatt gcaatgatca
    41641 ggacaaggac catgatcaaa taaaggggca aacttacgcc ggtaattttt gttgaccaaa
    41701 acccttcttt tttcttttcc tcaactgcag ttgcggttgt tgttttttgt gccattttta
    41761 cagatccttt cttattctgt atcgtatgaa aaaagccaaa gagaagcggc aaaagagcag
    41821 tagttggctg gctgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt
    41881 ttctgtatgt tcttttacta taacaatctt aatcttctaa agatttatat tcaggaaccc
    41941 atttagcaga ttcaacagct tctttgatat ccgtgatttc ttcacgattc aatttttgtg
    42001 caacaactga ttttgcgaca gtttcagcaa tcgtttgaga gaattcagtg atttttgcta
    42061 ctggaggcaa gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac
    42121 tagcttgaga aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagcc
    42181 ctaaacctgg gtacatcaac gcattatttg cttgaccgat ttggtaagtt acaccgttat
    42241 attccacatc agcagcaggg atacctgttc caactaaagc ttttccgtcc gtccattcaa
    42301 tcaaatcttt tgcttttgct tctgccaatt ttgttggatt agacaaaggc atgatgattg
    42361 gacgtggggt atgagccgcc atttctttca cgatttcttc agtgaatgct ccaggttggg
    42421 tagaagtacc gatcatgatc gttggatgga ttgcttttac aactgcttct aagtttgtca
    42481 attcatcagc attagtgaat tctgagcgtt tacgagcgaa tgggatttga ccaggagtca
    42541 atccttttgt atcttcaaac aataaaccct gtttatctac ttgatagaaa tgttggcgtg
    42601 cttcttcttc aggtacacct tgacggatca tttcatctaa aaggatgttt gcgataccta
    42661 caccagcagt accagcacca aatgtcaaga tcgtttgatc tttcaattgc tcacctgata
    42721 tattcaatcc acctaataca ccagctagta caacgattcc tgtgccttgg atatcatcat
    42781 tgaaagtagt gattttatct tcgtattttt ctaagatcgt tgcagcattt cctcgtccaa
    42841 aatcttccca atgtaataac aattctggga ataattcagt agctgattga acgaattgat
    42901 caatgaattc gtagtaagtt tcgccttcta cacgagcatg acgattccct aagtataatg
    42961 gattattcaa taattcttcg ttattagtac cagcgtcgat tgatacaggc aagacttgtg
    43021 caggattgat accagccgca gcagtgtaga ccatcaattt accgatagcg atgtcaacac
    43081 cgttcacacc ccagtcaccc atacctaaga ttccttctgc atctgtcaca acgatcaaac
    43141 gaatgtcgcg tccatctgct gcgtttttca aacttgcttt gatattttct ggttcgtcga
    43201 ttgaaagaaa tgcagcatct tgtggtttca agaaaatttc gttgtattgt tcgattgaat
    43261 ccgcaacgac aggatcataa acgacaggca tgaattcaac taaatgttcg cccatcaatt
    43321 tataaaacaa tgtccggttc gtattgaata gattcattaa gaagatacgt ttttccaaat
    43381 cggtctgttt gcttaagtat tgaccgtaag cttggacaga ttgttgttcc aatgtttgga
    43441 cagtacttgg aagcatacca gtgattccgt attttgcccg ttcttctttt gtaaaagctg
    43501 ttcctttatt tagaaaagga ttatttaata agtttagccc tgtaagcatg aatgattcct
    43561 cctaagtttt aatacaaacg aagtatagga gtcagatgag gatatagtca aaaagagcag
    43621 gtataataaa ttttatttat atgagtaata gagtcaaaag aaatcaaaat agcggtattt
    43681 gttcttttta tatcaacaat ccttgcttga ttactatgcg tatctcttat atgctaaaga
    43741 aagaaaagta gaagaataaa ggatatgtta gatagataaa tagaatctct gaagaataaa
    43801 tcacgtgatc gtgacgataa aatacactta cgtcaggtga taaaaaatac ataagtagaa
    43861 aactaagagg aggaacagaa aatgaaagaa gttcaattga tcagaaaaag cgaactatca
    43921 gaaggtggtt gcaatgcatg cggcgtagta gaagcaacaa gctatacatt aaagcttggt
    43981 gccaacaaag cgataatctc tgagttgaca gtaggcggtc ttgtggacag tctggcgctt
    44041 gctgaaggtt ttattggaga agatatttat gagatgtttt cagaaatccg tcagttaaaa
    44101 aaaggggaaa actgcataga ggttcatcat gagagcccga atgtgagatt caaacgtggt
    44161 gataatgaga tgatcttcaa taatcatgtg tccgatcaca ccgagctata tggaatcgtg
    44221 aatcaaatac taactgagct ttttggattg ggaccttatg cgttcaagga agaaaacggt
    44281 aatccaaaat taaacgaaga atggcaagaa acgattgaaa tacaaagaaa caacccgcac
    44341 ttatttcaat aggcatccac agtatttcgt aaaagggact ttgacacgac ttttgtcaaa
    44401 gtcccatcgt tttttatcta tttgtgttct aaacatttct ttcagagtag taacaactgg
    44461 aatgactgaa atgtaaacag attatttgcc tgtatcaata cggaagagta atataaagtg
    44521 tgtaaacgct atcatattat tcattcaatt ttttatagag ggaaggcatt gaaatggaaa
    44581 aaacgtatca agcaggtaca aatgaaggaa tagtggattt tatcaatatg gaagatttag
    44641 aaatagccgc ttcacaagtg attccagctg gtggttatgg ttacatcagc agtggtgctg
    44701 gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcagctg attattcctc
    44761 atgtattaaa agatgtagaa ttacccgata caactacaca ttttgacaaa gaaacgctga
    44821 ctgcaccaat catcatgaca ccagttgctg ctcatggctt agctcatgtg aaggcggaga
    44881 aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt tcttacgctt
    44941 cttgtacatt agaagagata agagaagcag gcggggagaa agcaccgcaa tggtttcaat
    45001 tttatatgag caaagatgat gggatcaatt tggatattct tgaagtggcc aagcgtaatg
    45061 gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt gaaacagacc
    45121 gcagaaatgg gtttaccttc ccactgccaa tgccgatcgt tcaggcttat caatcaggag
    45181 taggccaaac gatggatgct gtgtacaaat cttccaaaca gaagcttagt ccaaaagatg
    45241 tagaatttat tgctgctcat tctgatttgc cagtctatgt gaaaggtgtc cagtcagaag
    45301 aagatgtcta tcgttcatta gaatctggtg ctggcggaat ctgggtatct aatcatggtg
    45361 gccgtcaact agatggtggt ccagcagcgt ttgattcgtt gcaatatgtg gcagaagcag
    45421 tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgcggacag catgttttca
    45481 aagcaatcgc atcaggtgca gatcttgttg ctattggcag acccgttatt tatggattgt
    45541 ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca gaattggaaa
    45601 tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata aaattgcgtg
    45661 agaatcgttt tatttaagaa acaaatgacg aaaaaaatag gagtgcggca gatgatgccg
    45721 tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac gaaaaaataa
    45781 aattgaaata ttttttcata cttaataaaa aaggaagaat attcatgtaa acgattttat
    45841 tgtttttttc tcttttatac aaaaagggtc ataatttcaa gtaagcggat gaattcatga
    45901 ggattttttg catactgtct ctactatact taggtcacta agaaaaagga gtgatttatt
    45961 atgaaaaaga acatgattgg gacattatta ctttcaacac ttcttctagg cggcttggca
    46021 acaccagctt ttgctgaggg tcaagcgact tcaaaaggag atattacctt tactgaacca
    46081 acaaataccg tggagcctct taaccctact gatccttcaa aaccagtaga gccagcagat
    46141 ccggaaaatc cagcaactgg ccagacaggt cctttaacac tggatgtagt tcccgaattg
    46201 ccatttggta cacacgagat tgaaagcgga acaaaaacgt atcaagtaga cgcttcaaaa
    46261 aacgatacac cttatctcca agtatcagat cgtcgaggtg taggtgcaga tggccaagca
    46321 caagggtgga acgtgacagt ttcggtttca gattttgtaa acggttcaca agttttgcaa
    46381 ggcgctgaat tagattttgg aacatccact gttaaaagta cgtcagataa cgaatccact
    46441 ggaccaacta gtcaaactgt aactgggttg agtaaagcaa gtgcagcgac accaatattt
    46501 acagcagcaa aagatcaagg tctgggaaca tggctttctg tttatgatcc agcaaatatc
    46561 acactgaaag tgccgaaagc agcagctgga acattcacgg ctgatttgac ttggaacctc
    46621 gtggctggcc cagtagcata gagtatgaaa taaattcaac aaactgcggt gaaaatacta
    46681 taggtgaagg attttttacc gcagtttttg ttgaaaggat gtggtggaca atgaatcaaa
    46741 gtaaacgact gtttgtttcg tttttttcta ttttaagtct atttttcatt tttccgtcaa
    46801 tcagccaagc aaaggattca gcaggagact ttggaatcaa accggtattt ccagagaacc
    46861 aaatagataa agcaatcggt tattttgatt tattagtggc accggaacaa aatcagacac
    46921 tagaagtaat tatcagcaat tcttcagatg aagaacgaac atttgaggtc tctgtcaatc
    46981 cggcggtaac gagtgatggt ggaacgatcg attatagtca aaaaaaccca acgttagatg
    47041 agacattgcc gtttgatgtc cgtgacgttt tgctcattgc gaaaaaggaa atcaatgtat
    47101 cagcacatgc agaaacaact gtacctatag aagtgaaaat cccagcaaaa agctttaagg
    47161 gacgtgtatt agcgggtatt catgtttcac ctaaagaaga agctgaaaca gaaaatgcta
    47221 cagaaggagc acaaatcaaa aatcgtattg cttataatct cgcagtcgtt ttacaagaaa
    47281 gtcaagagac gatagaaccg gatctgaaac ttttatcagg agatttggac gaggtcaacg
    47341 caaagccaac tgttcagctt cgtttccaaa atcctcagcc taggattatc agcaatttga
    47401 tatttacgag caagatcttt tatgaaaatc agctttatat tgaaaatacg tctaatgcat
    47461 ttttagttgc accaaattcg aattttcact tgaatcttga tttagctggt gataaagcaa
    47521 aagccggcga ttaccgcgca gaaatcatag caaaatcagg cgacagtaat gaatggcgat
    47581 ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa agtaaatgaa aattccgttt
    47641 ttgcagtaca agaacaatct ttcccatggc tttatgcagg attggttgct gcagttctag
    47701 tgttatggtt gattttgctg tttttgtggg tccacaagaa aaagaaaaaa ggagaaacaa
    47761 catgatgagg tgatttcgtt gaaaacatcg atcttattgg gagggattat tgcaagtttg
    47821 ctgttcttca gctggcaaga gccagtccaa gcagatacca aaagttcagg tacgattaca
    47881 ttttatgatc taagtccaat tccgcatcca agtggttcag tcaatggcgc atcaaaacag
    47941 cagacacagc taccgattta taaagaatct aatcaacatg gcgtattccc ggagacgaat
    48001 gaacaaaaga tccgttttct ttatttgatc attggtatag aattatggct tcttgcgtgg
    48061 ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg ttgctacaaa ataaattttc
    48121 attttttcgc tccttctttt ttacttcgct ttttgtttta agtatattta tcttttcacc
    48181 ttcggcagaa gctgttggag caacgcctcc tgccatttct tatgatccat acacgtatga
    48241 aaatatcgta cctccaaatg gtggacgtag tgcagtcaat ggcgggaagc ttgtccaact
    48301 aactggggct aaccaattgc ctaaattgac tgctacttat ttgaacgatg gttcaaatga
    48361 tccttggaaa ggtggcacgg tccgttttca ttgggaaagc catgtgaaac taacaaatgt
    48421 acttgatttg gtgaatatca ctgcacaagg atttgcattt gtggcgatca tcaatttgcc
    48481 ggaacatgta tcaagcagtg atgtattaaa agcaattgac tggaacaacg cctacttgta
    48541 tattgccggt gcccccatca aggttactag cggaagttta ttatcagcag gaagccatac
    48601 cttgcgtttt gggatggggt cgtgggatac aaatactcta ttgacagata ttattgctat
    48661 tattacgcga ggcgaattca acgtggacga tatcccgctt caatttgatg ttacagctaa
    48721 tgtcgcgaag atgacagaaa acggagataa atatgatacg agtccaaaca aaattttgac
    48781 caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt ttttctgttg atttttatga
    48841 ttcgaataat attatccaag atcgtatgtt caattcgatt ttagctccag ctgcaggtag
    48901 attttatcct aaattaactg gcggaaactt gaatccagga gcatatgcat tttctgcaag
    48961 tgcagagata aatacgtgga atagttacat ctcaccaagt gattcaacag gaaaatataa
    49021 tacatttgct gataccacag aatacgtgga tggaaataac cagaaccttc caggctctac
    49081 caatagtcgg acattgacga tggggctgac tgaaggacgt tttgatcagt tgccatttag
    49141 tcgctttgat cgcgtagtca actattttac aggaaaaaac gttacacaag gagcaatact
    49201 gactcatact ccagcaagca caagtggatt gaatcagaca gccaatatcg tgtacagtgg
    49261 gaaagatgcg caaggcggat tgctttctcc tgttgcttta aaggtattgg agcagtacaa
    49321 gctagacgga acggttttac caacaaactt aacagccgat agtcagtggg gaaagctcct
    49381 ttcttctgca tcgtaccaag tcacggccaa ttggtcagca actgaactaa aaaagggttc
    49441 gatccattac aggctttatt cgaaaggaac ccaaaaatta gcgacaggat ggacagatca
    49501 acttttccaa acgatccaat caaataacgg gggtgtaaat acagctactt ctacactacc
    49561 tgcattggct tcagggcaat attattttga ttatcgcttg gtggacgatg tacttactaa
    49621 catgtatccg tcttttgcgt acaaatggca gtctgaacaa gctggaatca ccactcttcc
    49681 tcaaataaca gtggctaatt ttccatctgt gagtgcagat agtcagctaa aaaaccttag
    49741 cagacaacct gagacaggag atcccctgtc tgctctatca ggagatacga ttcaagaaaa
    49801 tgtgacattc acgttaacaa aatctgggga tgcgataacc gacaaaaaaa tcacgatctc
    49861 acttcctgaa aatactactt atatcaatgg ttccttaaaa ttaaatggta caactatcgc
    49921 agataccggc attcaacaag gagtttctgt cccagcagat ctattgagca aaataggaga
    49981 taccattcat cttacttaca actatcaatt aaatactgta gatacttctt tacaatccgt
    50041 cagcatactg acaaaggcag ctgtactttc cagcaatatt acattagcag atggtgctaa
    50101 gcttcctaac ccagttgtcc aaacatctgc taaaacaatt cttgtcccca aacaagaatt
    50161 aacattagtt aatgtaccag atgattttac atttggtaac gatctcccca aaccgttgaa
    50221 gacaagttat tatgaatcaa aaggcgattt ttcttttgat gtgcgggata ctcggcttcc
    50281 aagcacgtct ccttggcaac tgacaggtac attgacttct ttatttaaga atgatcaagg
    50341 tcaagaatta agcggaacaa aattatactt caatcattct ggatcaaagc aattgattca
    50401 gcaaggtcaa aacacgttga tttatgagag tgatggaaca gctaaaggag aggttctcgt
    50461 tgactttccc gataccgatg gtttactctt agaggtaaat agcagtacga atgctcaacc
    50521 tggcgcaacg tatcaaggca tggtcacatg ggaactgaca gctgggccaa ccagctaagt
    50581 gattagaatt tgtatggatc tgtgataact gtcacagatc ctctttttca aggatgagaa
    50641 ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt ggacttggaa cttttcagtt
    50701 tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca acttttatta gaggaaaaag
    50761 gagaggtttc cgttaataac gtacttaccc gcttatcagt tgatcgggct accttgaaag
    50821 aagatctgtt ttatttacaa gctaacttgg aaaatatcaa acaattggaa attgttatcc
    50881 aagaagggaa tattttatta atcagacatg gaaatatagc agcagtcgat gtttattatc
    50941 attgttactt gcgctattct acaaaatatc agatacttct ttatctgttg gaaaatggct
    51001 catttgatcg gcagaaactt gtcatggaac ttaatatcag ttcggctact ttgtctcgca
    51061 gagtgaagga attaaatgat tctttaaatg agtttcatct gaaaataaaa agcgggcggc
    51121 tgaccggtcc agaaagtcaa attcgttatt tttatttcca attattttgg tttggacgtc
    51181 cctatcttga aaacaaaaaa gaatacagtg atttatcagg aaacactttt ttagatctgc
    51241 tgcaaaaaga gtttagtttt gctattacag aagaaggacg attgaaattt gaaatatggc
    51301 tttccattat gaagaagcga atgcgctgta cgaaaaaatc caaaccagat tatagcaatc
    51361 ttcctgttgg attgtttcaa acgacacaat ttttattgga cctccataca tttttgaccc
    51421 gttacttctt tcaatatgcc tttccttgga atcaaaatga aacgttggtt ttttatcttt
    51481 tcatgatcag taactttaca ttagatacac ataactcgca tgtccaggag tttttgataa
    51541 gcgaaaaata caaagacgac gtagtagctc aattgaatcg aacgtttaaa caatcgatag
    51601 aagaatattt ctctttttat gtttttccag aagagatttc gcaaaagatt gcgttgacag
    51661 tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg gatgaatatt tttggccaag
    51721 aaggattaaa acagcggtta actgaagtag aagataaaca attcctggtt ttatgtgaaa
    51781 gattggtcag acaaacattt gctattgttg attttactga agtagacgag gaaaatgccc
    51841 gctttgaatt atttgctcgc tatgcatcta ttttacggac gctgatcaag cagttagaat
    51901 tacgtctgca aattgcttat gatcttcctt atgaaaggct aatggcggat atcgtgaaag
    51961 aaatcatcca ggaacaattg ctgaatacga tcaaatatga tttagtcgaa tacgagaaag
    52021 aaaagcagta tgatatcatt ttgactagcc aattgaaaga gtacccgtca cagaagtccg
    52081 ctaaagtttt tgtattcact agcaacgaat ataattatga tttcccctat ttgaaccaat
    52141 ttttaaaaga atgttattta gaaaaattga atttaaggat gaataaaaca tgaaaatcgt
    52201 gatcattggt gcttcccatg aaggcatcac agctgcccta aatctaagaa aacttcaacc
    52261 gaaagcagaa gttttactga tcgatgaaga tcataaagat ggactaggct atgtttcaaa
    52321 tggaatcaac ctttatttaa aaggaaagat acgtagttta gctgaagttg cccataacat
    52381 gcggactctt caagaaagtg gggcaaaact gatcaccgaa tggagagtaa ctgaacttga
    52441 tccagacaaa catcagctca tcctcgcatc caaagaagga aaaaacgaga ccataaccta
    52501 tgataaatta atcgtagcga caggttcctc tcctgtcacc ttgtataaac aaattgaagc
    52561 tgaaaatgtc tacacctata aaaatctagt gcaatctaag caagtacttg ccgctttaaa
    52621 agaagcaaaa gaagtagtga tcttcggtgc tggttacatt ggattggaat tagcagatgc
    52681 tttgagaaat aaaggataca ttattcatct agttgattat atgccgaatg ttttatcaag
    52741 atatttcgat aaggatatga tcaacagttt tcaaaatcag ctgcaaacta agcaaataaa
    52801 tttttatcca aatgaattct tgatcgattg gaaaaaatca gaagagaaag tagtatccgt
    52861 acaacttctg tctcaaacaa taaaggcaga tatggtcatt ttttctgccc aaactcgtcc
    52921 taatacgaca ttgttaaaag aaaaagtggc tttgtatgag gatgagactg tgatagtcaa
    52981 tgaatatctg cagacaagtg atccggacat ctatgcgatt ggagatatcg tcccagtcag
    53041 ttttgacaaa aataaacggc atctcttttt acctttagtt actcgagctg tacatatggc
    53101 aagagcagta gcgctaactt tgagtggtca gccaaccgct tacgacctca gacaaaagat
    53161 aacagcagca gtcattatag actacttttt aggaactgta gggctgacag aagatgaagc
    53221 gccattcttg gaacaaaata ccggttcgtg ttcaggagaa tttgatctat ttccacaata
    53281 cgacgaagaa aacaaaacag taaatgcaaa gctgatctac catccggata cattagaaat
    53341 aattggcgga cagctgataa gccaagaatt cttattatca gatttgaacc tgcttgcgga
    53401 tatcgtgaaa cacaaaacta cgattccaca gctagcagtt gaggattttg gatttctgtc
    53461 tgaatacacc tctcgttttc actacctcaa tgagttagct tttaaagtac tagctaagag
    53521 agcaaatagc aatagagtag ataaaagact gtgagaaata tcacggtctt ttattgtgtg
    53581 gggatataat aaatattaat caatcgagaa taaaaaattc atataatgaa atagcggaag
    53641 aattttttga gcataatatt cgccaaaaat ttatcaagtg ataaaattgt tgtataaaat
    53701 gattatttca ggaggaggag ttaaatgttt ttagcattaa atgaaattat gcattcaaag
    53761 ttacgttatg cgttagttgc aggggtgatg tttttgatcg cctacctggt attttttttg
    53821 actgggttag cttatggctt agctcaagat aatcgaacag cagtcgacaa gtgggaggca
    53881 gattcgattg tgctttcgaa agatgcaaat tcaaatctag ggatgtccat gattactaag
    53941 aagatagcag aagaagtcga aggtgggaaa gtggcgtatc ttgcccaaac acctggagtc
    54001 gtgactagta aagacagtac agaagaagga aaaatcaatg ttagcttctt cggtatcgat
    54061 aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag cttttgataa cgatgatgag
    54121 gcagtaggcg atattagttt gaaagaagaa tatggtctag ccataggaga tacagttaag
    54181 ttatctggta gtgacaagac gtttaaacta actggcttta cggatcatgc caaattcaat
    54241 gtctcaccag ttctctatac aaccatcaat gcttaccaag aaatccgctt cgaaaaagaa
    54301 gatacaagtg aaaatgcacg gattaacgca atagttgtca gaggtaaaat cagtaattta
    54361 cctgaagatt tagaacagat caaaatttct aaattcatca atgaattacc tggatataat
    54421 gcgcaagtat tgacttttgg atttatgatc ggatttttga tcgttattgc ggctattgtg
    54481 atcgggattt tcatttatgt actgacgatg caaaagatta atatctttgg tgtaatgaaa
    54541 gcacaaggga tcacaggagg atttattgcg cgttctgtag tggcacaaac attcatttta
    54601 tcatttgttg gtattcttct tggtctatta ggaacagtgg gaacatccct tgttctacct
    54661 gatgcggttc cattccaaag caattggctg ttttttggcg tcattagctt gttgatgtta
    54721 gtagtagctg tgttaggcgc actattttct gttcggacaa tcgtaaaaat cgatccgttg
    54781 aaggcaattg gataagaagg aggaagaaca tgaaagcaat agaatttaaa gaagtagaaa
    54841 aaagtttcca agatggcgac caagtagtta aagcattgaa agaaacaacc atgagtatcg
    54901 ataaagggga atttgttgca atcatcggtc cttctggttc aggtaaaagt acttttttga
    54961 ctttggcagg cggattgcag acgcctagca gtggctctgt atttatcaat gatcaagaat
    55021 tcagtaatca aaaagaaagc gaacgcgtta aactacgttt taaagaaatt ggctttattt
    55081 tgcaagcttc caatctggtt ccttttctga cagtaaaaaa gcagttatta ttagtagata
    55141 agattaaaca tgaacatcgt caaaaagaag caaacgaatt atttgagcag cttggcgtgg
    55201 ataagttgat caacaagtat ccagaagaac tatctggtgg agaacggcaa agggtcgcaa
    55261 tcgctcgtgc attgtacaat gatccgtcca ttattctagc agatgaacca actgctagtt
    55321 tggattcaga aaaggcacga gaagtagtcg atatcttggc aaaagaatca aaagacaaaa
    55381 ataaagcaac gatcatggtt actcacgata cacggctgat tgacaaatgc gatagaatat
    55441 ttatgatgga agatggtgta ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg
    55501 gtccatcgat acaacaaaga tacacagcaa aaaattttga aagaagcgac tgacttgttt
    55561 atgtccaagg gatatctcgg cacttcaaca agagaaatcg cacaaaaagt tgggatcacc
    55621 cagcccaatc tctatcatta tttcggtgat aaagaaaaac tatatacggc agttttagaa
    55681 aaccacttaa aagatgttgg aaaagctttg agagaaatcg ttcaaacaag cgaaacggga
    55741 tttcaagtaa ccttgacaaa aatggcgcag tttttgattg agacacatct ggtcgatttg
    55801 tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc
    55861 tttctatgga agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct
    55921 gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc attttttctt agtgttagca
    55981 ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc gcaattgatc
    56041 gatttatatt tgaatggtgt attaggagaa gcgtaaagct gttatatagg gagaaaatag
    56101 ttaggtgaag aagcaagata tgacgaagat cgttatatct tgcttcttta tataggtagt
    56161 ttttcataaa tacaaatttg ttattctctc atgattatca gggtatattt ttatttatta
    56221 tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt ttgtagaatg
    56281 aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag atttcaggat
    56341 atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt tatatctttt
    56401 acagcccctt ttttgcttac ttaagctgat tttaagtttt ggcccttagt ctggatctac
    56461 gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa tcattatcat
    56521 tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac aaacggtcag
    56581 cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag aacaagagac
    56641 attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc taactacccc
    56701 acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa aaaatgctta
    56761 tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg gaaccaaagt
    56821 cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg tattagatcc
    56881 tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag aagaaaaaga
    56941 tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg gagcaacggt
    57001 ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg actacagtga
    57061 agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat atgcgaatga
    57121 agcaactgga atcacgacat attattacta tggacaagag gaaacattag cacagacaat
    57181 tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg ggaactatca
    57241 agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata tgaataatga
    57301 tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg caagtttgac
    57361 acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa aatagctcct
    57421 catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttaaac accgtttaag
    57481 gaagaggtcg tgagcctgcc gttcaactct gagtcacaag gagcaatttc aaaaaacagc
    57541 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag aacaccgttt
    57601 aagaaaaagg ttgtgaaatc aacgttttgt tctgagtatt agagaaacaa acggaaaaat
    57661 agttcctcat attttgccgt ttgttgagct aataccgagg atcagatgat tgaacaccgt
    57721 ttattcggaa caagggactg tgacatgact tttgtcacag ttccattatt tttttagtag
    57781 cttcaaagct ttttctgtat tggcaaaatg aagtttggca tatttttcaa gcataggcat
    57841 gccgcctttc tccaatagtt ttgccgctac ttggtcagat ttcgttccag ctcctgacgg
    57901 aagggtgatt ccagcttcag ctgatgcttt gtctaactct tccaaaaagc tggcacgact
    57961 gatgatagaa gcagcagcaa cagctaaatg atattgttct cctttagtaa cgaagtacag
    58021 tttttcttgt aattgatttt tttctaaacg cgcatatttt ttaaaatttg cttccggcgt
    58081 aaactgatcg atcaaaatcg cttctggttt ttctggctcg atttttttta gcagcaaatg
    58141 gatcgcttga ttgtgcaaag cgactttcat ccgaacggca ttgtattttg gttggatctc
    58201 attgtatttt tctggagata agacaagcag tttatagggg atggtttttt ttaagatgtc
    58261 agcaagctgg atgatttggc tatcttttaa ctcttttgaa tctctaacac caagtttacg
    58321 taattctgca agctgatcac gtttcacata agcagcacat actgttagcg gaccgaaata
    58381 gctgccattt ccgacctcat cactacctaa aacggataat tgactgatat tttttggcaa
    58441 agaagaggct gtttttgctg gcttggcttg tttatcctgt gcctcccccc atttatcagc
    58501 ttctattgct gcattcgtac cttgaaacat cacttttccg gatgtataag cggtaatggt
    58561 cgtcccgtct ttttttgcag caaagctagt ataaggaatc tgtttattta acaaataaga
    58621 gcgatagtat gctttcattt gactaagttg gtcgtttgtt gctttgataa tgacgttgct
    58681 cataaaattc tcctcgtttt tatacataaa actataccgc aactcacgac ttttcgtttt
    58741 ctaagagatt gcgagacata gcattttttt aaagtttatc atataatagt taagtatgtg
    58801 aaaaatattt gtggagaacg gtgaaagaac caacttcatg gtacactaaa taaaactttc
    58861 atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa agcggtgatt
    58921 gccaaccaaa catatactat tatcggacgg gaaaccaagc atcatatgga tatcgtaacg
    58981 aagctgatca atgaacagtt agcagaattg aaacaattat caccacaaat ggataatgaa
    59041 caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga
    59101 atattggagc tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa atttacagag
    59161 cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg aagctcggga agtattgaaa
    59221 gaaaatggtc aagcagatca taccattaaa aatcatgtgg aagcacaaca gattttaaac
    59281 gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt aagatccttt tatcaaaata
    59341 agaaaaattt aaaaaagtta gaagagaaag gaacgtagca tgctgagttt gctgatttta
    59401 tttttattgc taattgcgtt tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata
    59461 ttcgcgattg gatatgtatt gtcatttatt gctgcccagc atttttacaa accattagca
    59521 agtcatttgg aattatacat tccataccca gctgtcaccc caacatcaaa gttagcattt
    59581 tttgatcagg tatttgcctt tcacctagat gaagcttttt atgcaggaac tgcttttctg
    59641 attattttgc taattggctg gctgcttacc cgatttgtgg gggtttttgt ccatggattg
    59701 acgtatgtgc cgatcttgag acaggtagac tggattgcag gaggcatcct gtcactaatc
    59761 atggcttacg ttactatatt tttgattttg cagttacttg cctttgttcc attagatgtg
    59821 gtccaaaatc aattcggcga gcatagtttc gctcgtttta tcgttgagag aacgccattt
    59881 cttaccaaca agatgtatga gctttgggta acgaatattt tgaattaagg aaaatagtgc
    59941 tggaaaatat ttcgtttttt gtacagagta gtttatttaa gaaggctgcg tagcgtatgt
    60001 ccggcacagc tcttttttca tagtgaaaga taaggtgata aaatgaataa ccgaatcctt
    60061 gaaacattgg aatttgaaaa agtcaaacag atggtcagac aatttgtagt gactgcacaa
    60121 ggaaaagaag aactggcaga attggtacca gtgagtgaga aacaaacgat cacaaactgg
    60181 cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc gaggcgggat ccctatacct
    60241 aaaattgaaa atatccgtcc ccatatgaaa cggattgaga tcggagcaga cttgaatgga
    60301 gtggagcttg cacaagtttc cagagtattg tctacgacaa gcgaattgaa tcgtttcatt
    60361 gatgatcttt ctgacagtga aatcgaattt gcacgtttgt atatgtgggc agatcagtta
    60421 gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa tcgatgaaga cggacgcgtg
    60481 actgatgacg cttctcctga gttaaagagc ataagacaga acattcggcg aagtgaacaa
    60541 gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa atgcaaagta tttaagtgat
    60601 gcgatcatca ccatgcgaaa tgaccgctac gttatcccag tcaaacaaga ataccgcggt
    60661 gtatttggcg gtgtagtcca cgatcaaagt gcttcaggtc agactttatt tattgaacca
    60721 aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc aaatcgctga acgaaatgag
    60781 attcaacgta ttttaagtga actttctgct gaacttgttc cccatcgtca agaaatcatc
    60841 cacaatactt atgtgattgg taaaatggat ctgatgaatg caaaagcacg atttggtaaa
    60901 gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc atgtcgtctt gaaacaagca
    60961 agacatccac tgatcgatca ggaaaaagta gtttctaatg acatcacaat cggaaaagac
    61021 tatcaagcca tcgtcattac aggacctaac acaggtggga aaacgatcac gttgaagact
    61081 ttaggtctgt tacaactgat ggggcaagca ggattaccga ttcctgcagg tgaagaaagt
    61141 caaatcggta tttttgagga agtatttgcg gatataggag acgaacagtc gattgagcaa
    61201 agtctatcga ccttttcttc tcatatgacg aacactgtag atattttatc aaaagtcaat
    61261 gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa cagatccgca agaaggtgct
    61321 gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt ctgcttatgt gatggcaaca
    61381 acgcattatc ctgaattgaa agtttatgga tataatcggg caaatacgat taatgccagc
    61441 atggagtttg atgtggatac attaagtcca acgtatcgtt tgctgattgg cgttcctgga
    61501 cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg atacagaagt tattgatgaa
    61561 gcgaagcagt tgatgaatga tgagagccaa gatttaaacg aaatgatcac agatttagag
    61621 aatcgccgga aaatggcaga gaccgaatat ctggaaatgc gtcattttgt ttctgaggca
    61681 caagaacttc acgatgattt gaaagaagct tacagctatt tctttgaaga acgtgaaaaa
    61741 gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt cagaggcgga agaaaaagca
    61801 gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga ttgggcaagg aaatgtgaaa
    61861 gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc tacatcaaga agaaactttg
    61921 aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa cgttgaaacc gggagacgaa
    61981 gtgttggtta cgacttacgg ccaaagaggg acgcttttac gtaaaaacgg gaatcagtgg
    62041 caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag acgagctgac gcctgttgct
    62101 cctcaaaaag agccgacaca acgagttatc catgctgtac gatctgaatc tagtagtcac
    62161 gtgccgaatc aattggacct tcgaggaaaa cgatacgaag aagcgctaag tgaggtagat
    62221 caatatttgg attctgccat tctagctggt tatcctcaag tgactattgt ccacggaaaa
    62281 ggaacaggcg cacttcgcaa gggaattact gattatctta aaaaccatcg gagcgtcaaa
    62341 agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg cgacaattgt taaatttaag
    62401 taaagcttac taaaggtaag cagatggttc gctatccaag tatagaaatg ctatactagt
    62461 catagatttt aaaaatggag gtctttgata tgtcacaagt aattacagat aagacattta
    62521 atgaagaaac agataaagga cttgtattga ttgatttttg ggcaacttgg tgtggtccat
    62581 gtcgtatgca agcaccgatt ttggatcagt tggaacaaga atatgatgaa gaagaattca
    62641 gaattgctaa aatggatgtg gatgaaaatc cagaaactcc tcaacaattc ggtatcatga
    62701 gcattccaac attaatgttg aaaaaagatg gtcaagtagt agaaaaagct gttggtgttc
    62761 attcaaaaga acaactacgt caaatgatcg atcaatattt ataaaaactt acagcaaaaa
    62821 taagctgagg gtgggactga atccagttcc gtcctcggtt tttttgcggt tgagttaagg
    62881 ttattcaaat taatattttt tgagtataat tacgttgtca ggctggattg tttttttgac
    62941 agacatgcta taataacttt aattgaatat gaaggggagt accagatgaa cgaacgaatc
    63001 aaaaacaaat tagctttact tcctgatcag cccggctgtt acttgatgaa agataaaaat
    63061 ggaacgatca tttatgtcgg caaagccaaa atattaaaaa atcgtgtccg ctcatatttt
    63121 actggtagcc ataatacgaa aacggaacgg cttgttagtg aaatcgaaga ctttgaatac
    63181 atcgttacag aatcgaatat cgaagcattg ctcttagaga tcaatctgat aaagaaaaat
    63241 gatcctaaat acaacattat gttaaaggat gataaaacct atcctttcct taagataacg
    63301 aatgaaaaat atccccgttt ggtgattact cgtaaggtac taaaagacaa agcactgtat
    63361 tttggaccat atcccgatgt cggtgcggcg aatgagacaa agaagatcct cgatcgatta
    63421 tttcctctga gaaaatgcaa acctagtcag acgaaggagc cttgtcttta ctatcatttg
    63481 ggacaatgcc tgtgtcctta ttatttcgac gtagatccga aagtctatac gaaaatcgtt
    63541 gatgaagtga aaaacttttt aaacggagga cacgaaaaaa tcgagtcaga gatccatcag
    63601 aaaatggaaa aagctgctga aaacatggag tttgaaaaag ctgcagaata ccgagaccag
    63661 atccgtgcca ttgagacaat catgactcgc caaaaaatga caaatactga tttactggat
    63721 cgagatgtat ttggttatgc gattgataaa ggatggatgt gtgtccaagt gtttttcgta
    63781 agacaaggaa agctaattga acgagatgtt tcaatgtttc ctttctataa cgaagcagaa
    63841 gaagatttct tgacttacct cggtcagttt tatcaggaaa atgagcattt cattcctaaa
    63901 gaagtcttga ttcctgatac gatcgacaag cccagtgtag aagctttgtt agctacgaaa
    63961 gtgctgcaac caaaacgagg agaaaagaaa aaattagttg aattggcgag taagaatgcc
    64021 tcagttgctt tgaatgaacg gtttgatctg attgccagaa aacaggaacg gacgatcgga
    64081 gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc caatacgtat cgagtctttt
    64141 gataactcta atatcatggg gacgaatcct gtggctgcga tggtcgtatt cattgatgga
    64201 aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa cagtcaacgg accggatgat
    64261 tatgcatcaa tgagagaagt catctatcgc agatattcgc gagtgattcg cgaagggttg
    64321 cctttacctg atttgatctt gatcgatgga ggaaaaggac aagtagatgt ggcaaaagat
    64381 gtcttagaaa atcaattagg aatcgatatt cctattgctg gcatggtaaa aaacgataaa
    64441 cataaaacaa atgagttgct attcggtaca gatttggctg tagtaccgct tgagcgaaat
    64501 tcaccagagt ttttcttact ccagcgaatc caagatgaag tccatcgatt cgcaatcact
    64561 ttccatcggc agctacgcag taaaaacagt tttgcttccc gattagatga aattgaaggg
    64621 ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca aatcgttgaa aaatatcaca
    64681 gcagccacga cagaagaatt acaagaaatc ggactgccga aaaatgtcgc acaaaacgta
    64741 tttgataaat tacatcagga atgaggaaag ggcactttac aaagaaagta aagtgtccta
    64801 tcattttatg tacaacaaaa agagcgttta cgttttgttg gtaaataata aatcgcttac
    64861 gtctggtgtc taaggatata tgtgatttct tgaagaagga gtgaagaaat gttagaagtc
    64921 aaggatcttg ttaaaacttt tgggaactac acagctgtcg atcatgtctc tttccaaatc
    64981 cccgatggaa aaatcatggg gctaattggc caaaacgggg ctgggaagac aacgactttt
    65041 cgtttgatct tggatttttt gacacaagac caaggagaag tgttatggaa tggccatcat
    65101 cttggggaga aagattatga tgtcatcggt tatcttccgg aggaacgggg actttatccg
    65161 aaagtaacga ttcaagacca actgctttat tttgcagaac ttagagggaa atcaagaaaa
    65221 gagattgaac caaaaatcga cttttggatg gaaaaatttc aggtaaaagg gaaaaagacg
    65281 gataaagtaa aatctttgtc taaaggaaat cagcaaaaag tccagctgat cgccacgtta
    65341 atccatgaac ctaaattgat cattttggat gagccattca gcgggttaga tccggtaaat
    65401 gctgaattgc taaaagatgg aatattggaa ttgaaaaaac atggttcatg tgtcattttt
    65461 tctagccaca atatggataa tgtagaaaaa atctgcgatc atttgatcat gttgcgaaac
    65521 ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg aatcatttgg ccgtacgaag
    65581 ttattcttgg aatctggttt aagccaacag gaagtagaag aaatcgaagg cgtgaaaaaa
    65641 gtgatccttc atgaagacaa tacgttagag attaccttag aagatcctga agctgggaaa
    65701 gaaatcttta ctcgtgcgac tcagttcggc tacattccta tgtttaacca gcaaccgcca
    65761 actttggaag agattttcaa attgaaagca ggtgaaatcg atgcgtaagt tttggattat
    65821 taccaaagat gtctatttaa aaaatgttaa gtcgatttct ttcttgatca tgatattagt
    65881 cccttttatc atgctgggta tcatttatgt agcaggaaac tttgctcaac aaaatagtga
    65941 tgtcaataaa atcggcgtat tggctgataa tgagcaaatc agtcagcaaa tgagccaagt
    66001 caaaacagat gattttgagt tcaaagcagt tgattctgaa gaaaaagcac aaaaagagct
    66061 ttcggaagaa aaaatcgatg cgtatttagt agtctcaact gagaatgata agataaatgg
    66121 gatgctttat agcgaaaatt cattaggaca aagtacacaa ctactgatac aacaacaact
    66181 gactggcttg caatcgatgc ttcgagcgag tcaattaggc atttcaccag aagaagtagc
    66241 tagcttgagc caacaagcaa gtttgactcg acaaaaagtc aattttgatt caaaaggaaa
    66301 aatgacagtc ggagaagata actcgacagt ccaatacgtc gtaagttatg tagcaacgat
    66361 tgtcttgttc attattattt tgacgtatgc gcagatcatt gcacaagaga tcgcttctga
    66421 aaaaggcacg cggatcatgg aagtgatcct gtccagtacg actgcacaaa agcattttta
    66481 cggaaaatta acaggtgttt tacttgttgc attaacgcaa atggcattat acggtattat
    66541 ttttggtata ggatacagtc aattcaaaga tatggaaatg gtcaaaaata tattagacgg
    66601 tatctcattg gataacttga ttggaccatt cttgtggtat tctcttctat tcatgatctt
    66661 tggcattttg attttttctg ttttagcagc tttatgtggc tctttggtca acaaagcaga
    66721 agatacagca aaagcaattt tgccagtgac ttatctatct ctaggcggat acatgcttgg
    66781 attgatacta ggagcttcag atccaaacaa tatcgttatt cggatcactt cgtatattcc
    66841 attcttatct tcatatatta tgccgattcg cttagcaaat gagacagtcg gtatgggtgg
    66901 agcattgatt tccttgttgg tacttgtagc cgtaacattc ttattgatga tcttgtctgc
    66961 aaacatgtac aaatcgaatg tactcgttta tagcgaaggc ggattatgga gttcattgaa
    67021 acaatcgatt tcaattatga ggaatgaacg aaaaaaagca taaaacaatc ctaaaataca
    67081 gaaggaagct ggacatctat tgttcccagc ttctttttgt atagacctat tttatttatt
    67141 ttactatatt ggatatatat tcattgaaaa tagtgtataa caactttata atattgagta
    67201 taccaatttt tggaggcgtt ttaatggacc gttatttaga tttactttca gaaaaattcc
    67261 cctcaacgga ggatgtcatc acggaaatca tcaatctcga agcaattttg cagcttccaa
    67321 aaggaacaga gttatttttg agtgatatac atggcgaatt tcctgctttt gatcatattc
    67381 tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga ccttttttct gaacaaatga
    67441 cggaaacgga tatcagccaa ttcactattt ttacggctta tccagaatat gcattaacga
    67501 ctgattggta caaagaacaa gataaatcgc agctgatcca tcatttgatc gatcttttac
    67561 ggtttacgac agtcaaatat acccggtcaa aagttcgaaa aggattgccg aaagaatata
    67621 gctatatcat tgaggaactc ttgtatatcg atgaccgtat caatggaaaa aaagactatg
    67681 taaaaaaaat cattgaacaa cttgttttga tgaaggaaga agaaaaattt ttaacgaaac
    67741 ttgcacagac gatccaaaaa tctgtcatcg atcatttgca tatcgttgga gatatttttg
    67801 accgaggaac tcaagcagcc aaagtgatgg atcgaatcat ggatttttct tctgtagata
    67861 tccaatgggg aaatcatgat attttgtgga ttggtgcgta ttgcggctct gaggcatgtt
    67921 tactgacact tttacggatt gcagcacgct ataattattt atttgaatta gagaaagaat
    67981 atgggttgaa tttacgtcca ttgttttctt ttgctcatca aacttatcag aagaatccag
    68041 tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga gcaagaagaa ttagaaaaaa
    68101 tccatcaggc actttctatc cttcaattca aatcagaaca tcagttattg gatcgtcgac
    68161 ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat cgactatgaa gcatcaacga
    68221 ttgatttaga aggtcagctc tatgagttga aaggaacttg ttttcaaacg atccagccag
    68281 atgatccaaa aaaattcttg gaagaggaac aaaaagtgat tgatagtttg atgtattcat
    68341 tccagcactc tttgagaatg caaaaacata tgacgttttt gatcgaaaaa gggggaatgt
    68401 atctcacgaa caatcagcat gtgctgtttc atggctgtat acctgtagac gagaagggac
    68461 agctcttagc atttgaatta gaccaatctt atcgcggaaa agaactgctg ggcttttttg
    68521 aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa agaagattta gcaaccgatt
    68581 tgatttggta tgcgtggagt gggccatttt cgcctttatt cggcaagagt aaaatggcga
    68641 cttttgaacg ttattttctc acagagcctc atacgcacgt cgagaaaagc aatccttatt
    68701 atcacttgcg agatgaagag tggttcagtg aaaaaatttt agcagaattc gacacaaaag
    68761 aagaagggtc agcaatcatt aatgggcaca cgccagtaaa agtaaaaaaa ggggagtcac
    68821 cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg tttatccaaa gcttatcaaa
    68881 aaacgacagg aattgccggt tattcactat tatgcaattc ttatggtttc caaatcgtga
    68941 cacattatcc gtttttgcct attgaacaac aatttgcaaa aaaatccgac caaacaaatg
    69001 tgaaaaaagt aatcgaacag caactaccac gcaaactcaa tcgtgataca acaaaaggta
    69061 tggaactgca gcagcagatt acccagctca aaaggctgtt ggactatcga aaggacgatt
    69121 gagaagaatt ccagtgaaca ttgttccata gactggagac tgagacaaaa gtcgtgtcgc
    69181 agtctctttt tccggataaa cggtgttctt ctgccaactc ctcggtcaca agtcacacta
    69241 ttcaacaaac atgaaaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc
    69301 aggctcacga cctcttggta taaacggtgt tcaaaagcca gtttctcggt attaagccaa
    69361 atttatgcaa aaatgtgaaa agccattttc acaaaatttt tcttaatact cgaaactaaa
    69421 cagctttttc acaaccccct cagaaagtct attaaaattc attcacaaag aaatcgaata
    69481 agcgctgttc agatggatgt acacggtggg taagttccgg atgccattga acaccgagga
    69541 ttttatgcat atcatctttg ctttgaaccg cttcaacgag tccatcgtta gaaaaagcga
    69601 tggcttttaa taaaggtgaa agatctttga ctgcctgatg atggtaagaa ttcacaaaat
    69661 atggatcggg gagaaggttg cttaacaaac tgtctgaaac aatcttgact tcgtgagtcg
    69721 taaattgcgg ggcagtgggc tgttgttcat gttttactga ccactttgga tataatgaaa
    69781 gatcttgata aagtgtacct tccaaaacga cattcaatag ttggagtccc cggcagactg
    69841 caaaaattgg ttttctttgt ttcaatgctt ccaagatcag tgcctgttca aattggtctc
    69901 gttgaatatt tgtttccgtt agctttggat gaggatcttc catgtaaagt tgtggactga
    69961 catcttgacc acctgcaaga ataagtttgt cgatttgtcc aatcagttga ggagctgaat
    70021 ctggagacat gactggcaga ataagaggca atccttgagc atctaaaact gcatccacga
    70081 atccttgcgg tgtatagctc acttgatttc cttggaaagt atctgttgct cgaatcagtt
    70141 gatttccggc aatgccgatg attggtttca tttgtattcc ccctaaagta gacttattgt
    70201 aacaaggatt taaaaaaaat taaactatgt aagacttgcc cctatactta tctgttcttg
    70261 ctgtgttact atctgtaaag agaaatttcg tgtgaaattc atgacgatag ttgaaaatat
    70321 tgcttaattt gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag
    70381 gatctagttc ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt
    70441 tatctttaga ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg
    70501 aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa
    70561 tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc tatgcaacag aaaaagagca
    70621 ggaattggaa acgatccaaa caaactatcg acaacaacta aaacagttgg tggaagaaaa
    70681 agaacaattt gaaatacaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga
    70741 caaggtgcaa gcaactttga aagatcaata tcaaaagcat cagagtcaag cgatcggtgc
    70801 gatcattgaa agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca
    70861 tttcagataa aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaacgttg
    70921 aaatccggga tctgtatcaa aatagaacaa acaaccagtg ggtggagaag tattttccag
    70981 ataccacgat tattgataaa gaagcaaaat taagcgcttt aggtaatcgg ttgaaagaca
    71041 tccaagaagc tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt
    71101 tgaaacgtca agaactttcc ttacaagatt tggaagccaa ttattccgaa gaaaatttca
    71161 taaataaatt gaaagaagtc cttgatttaa aagaaaagtg ggaacaacta actgaagaac
    71221 gtgatcgttt agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc
    71281 caagtatgta tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt
    71341 ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt ttatatggaa gaaatagatt
    71401 atacgccaaa acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgttatag
    71461 aagaaattgc tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta
    71521 aagaaaaact gcaagagacc aagcagcaat tatccattgt tcacgagcag caaaagaaga
    71581 taacgacagc gttaggtcag tgcagtggct atatccaaga atttgaatgg gtagaagaag
    71641 tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg gtttatccat gcgacttatc
    71701 tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca gaatttgctg catgtgttaa
    71761 acgaaaccgt atcagaagaa gatatatacg tcagtttcga aacaccaaac tcagaagaga
    71821 ttcagcagga agtccctact aaattgaaaa accatccgct tgtcgaacct tttgaattgc
    71881 tgactgaaat gtacagtctg ccaaaatatg aagaagtaga tccaacacct tggatgacac
    71941 cattttatct tgtcttcttc gggatgatgg tcgcagatgt tggttatggt ttgctgatgc
    72001 tcattgggac aatactagct caaaaactac ttgttctacc acgcgggatg aaacgtttcg
    72061 taaaattctt tgagattctc tcgataccat cgatcatctg gggattgatc tacagttcat
    72121 ttttcggaca gtcattacca aaagaactgt ttggcatccg cttgcctttc ccaatcctat
    72181 ctacgacgga tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga
    72241 ttttggttgg attatttgtg gcagcaaagg aaaatatcag gagaaaagat tacttgagtg
    72301 ctgtcagtga tggatttgcc tggcaaggga tcctaattgg gatcgttatt gcgttggttg
    72361 gtgccatgtt gataaaaaat acagcatttg tttaccttgg cgctgggcta tcgatcattg
    72421 ctgccttatg tatcttgatc gttccgatta tccaaacgcc gtctaaagtc aaaggtgctg
    72481 ccaaaggtgc gtataatctt tacggactga ccggctatat cggtgattta gtcagttata
    72541 cacgtttgat ggcattgggt atttcaggcg gaagtattgg agcggcattc aatatgctag
    72601 ttgctttcat gccgccagct gctcgattca gtgtagggat ccttttgatc atcgctttac
    72661 acgcactgaa tatgtttttg acactgctaa gtgcctatgt ccatggcgca cgattgcagt
    72721 atgtcgaatt ttttgggaag ttttatacag gaggcggacg tgcctttgat ccgctgaaaa
    72781 cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa caataaataa ttggaggatt
    72841 tttatataat gatggattac ttaattagtc aaaatggtgg aatggtattt gcggtattag
    72901 cgatggcaac agcgacgatt ttttcaggga tcggttcagc taaaggtgtt ggaatgacag
    72961 gtgaggcagc agcagcattg acaacaagtc aacccgaaaa attcgggcag gctctgattc
    73021 tgcaattgct tccaggcacg caaggtttgt atggattcgt tattgccttt ttgattttta
    73081 tcaacttagg aaatgacatg tcagttgtac aaggactgaa ctttctaggt gcttctctgc
    73141 cgattgcttt tacaggcttg ttttcaggta ttgcccaagg aaaagtcgca gcggcaggta
    73201 ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg
    73261 ttgaaacata cgcgatttta ggtttcgtta tttccttcct attagtgttg aatgcctaag
    73321 cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaacgaagc
    73381 agcagagcag gaaagagcgg cgttagaaca agaagagcga atgaagatcg atcaaaactt
    73441 tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa caaaaagaaa aacaaatcga
    73501 attactcgaa aaaaaatacc gtcagctaag gaatcgccag caagtagaag tacgacaaga
    73561 aaatttgaat gcgaagcagg aatttttaag acgattgttt gcagatgcgg taactgaaat
    73621 ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa aatgcattgt attcattacc
    73681 tttgacagga aaagtagcat tcattgctgg agaaaaatca gcagcctatc tttctcagac
    73741 gttgttggat gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt
    73801 tgctgatcaa gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag
    73861 cagccttgta caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga
    73921 ataaggaggg atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag
    73981 cttgaacttt tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca
    74041 cttggtgaaa tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat
    74101 gacttcgaac agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca
    74161 gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt atactttcca taatctgaag
    74221 gttttgacaa aagctgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga
    74281 ttttattcat tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaacttcct
    74341 gaaagcatta tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt
    74401 cagggaattg acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat
    74461 aagctaggat atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc
    74521 acaacgatgg ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt
    74581 tccagctcgg gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa
    74641 gattacacga attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt
    74701 gagggaaata ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg
    74761 tatcaagaag cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca
    74821 aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa
    74881 tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg
    74941 cgacaaagat tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac
    75001 caagcaaacc gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta
    75061 tgtcacagag caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat
    75121 gatacctgct atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga
    75181 aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat
    75241 agaaattttg caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa
    75301 catgtctgat gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga
    75361 aattatcgag atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat
    75421 tggtccaggc gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg
    75481 gatcatttca caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac
    75541 acaaagtaat ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg
    75601 gtggttcgaa ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat
    75661 cgtagaagaa acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac
    75721 gatcaaaaca atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac
    75781 cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc
    75841 aatcaaagaa aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac
    75901 attcttccca gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa
    75961 aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg
    76021 atgtggcgag cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga
    76081 ccctaataca ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat
    76141 gccagtagca gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg
    76201 tgacatgggt tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact
    76261 tcgtgagatg agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct
    76321 tggttcacgg cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga
    76381 aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc
    76441 ggaaccagtc acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag
    76501 tttagcccaa aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc
    76561 aacagaagta gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac
    76621 cgagggcatg cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg
    76681 gattgattcc ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga
    76741 agattatttg cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca
    76801 attcaaaatg ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt
    76861 aggtgcttat ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg
    76921 aagcaaatat atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa
    76981 gcaaacgatg caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta
    77041 cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg
    77101 aagaattaat cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag
    77161 tccaagaaga taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg
    77221 actcttctgt acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg
    77281 gtcgagtatt cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa
    77341 agcatttgga tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt
    77401 ttatccaaac aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat
    77461 taccagtatt ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac
    77521 aagcaacggt actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca
    77581 cttttgaaga agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt
    77641 ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta
    77701 tggctttgac tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca
    77761 tgacagacat gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag
    77821 ttccaggaag aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac
    77881 gagcaggaag aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc
    77941 cagaagacga taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga
    78001 tcatcttgac tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt
    78061 ctctttcgcg gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag
    78121 ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg
    78181 tagttttagg tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc
    78241 ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac
    78301 ttgatctagg gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag
    78361 acgacatgct ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg
    78421 tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag
    78481 ggcataaatt attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc
    78541 gaaagaacaa ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt
    78601 ttgtattggc aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg
    78661 cggaaaacgt tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga
    78721 tgaattttga atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt
    78781 ccagtgccga actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg
    78841 aactgacaga aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc
    78901 ggcgagtcaa tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata
    78961 ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa
    79021 atatgggtac cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca
    79081 aagcaaacaa ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct
    79141 cctgatttta gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg
    79201 gacaaatttt atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac
    79261 aacattgaat acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat
    79321 tgagatcggc ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa
    79381 gaaagtcagt ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc
    79441 aggtgtgatg aagctcatgc tttacatcct aaaattggcc tttgtgatcc aattgattgg
    79501 tacggttgct ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta
    79561 cagtttgttc catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag
    79621 tttagccaat caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc
    79681 tggtgggtta ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat
    79741 gacgattcat tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt
    79801 tgtgttcttt ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg
    79861 attggcaaac acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga
    79921 ttacctccat atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg
    79981 aacatctggg tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca
    80041 gatgcgttcg atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca
    80101 aggcgcagtg ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc
    80161 catcatgatt ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc
    80221 ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt
    80281 gactgcaata gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt
    80341 gactgtggtc ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc
    80401 agaagaaagt gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa
    80461 aataagaaaa taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt
    80521 tattttttga ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg
    80581 aatcaataag atttgattcc tttatttatc aataggttat aattcatatt tttttatttt
    80641 ttcttttacg atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat
    80701 tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat
    80761 accgttagca agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag
    80821 aattcgatag taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag
    80881 ctgattggct ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga
    80941 atggaggatt tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa
    81001 aattgcttgt tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa
    81061 tccagaacaa ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt
    81121 ttggaaagga ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc
    81181 ttcaataacc catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg
    81241 agagatttga aaccctatag gattattaca agaaggcata agaaagacac catgaatctt
    81301 cttttttcta cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat
    81361 tggaacgatc tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg
    81421 gtctgttgct attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt
    81481 ttgaacaccg gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa
    81541 ccaatttgct gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa
    81601 accaacaaga tttgggttat tactagttga ttgaataaac gggacaacga atttattaca
    81661 ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga
    81721 gatggtgatg gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata
    81781 cagcaaacct ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc
    81841 taaataatca gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt
    81901 taaaatatct tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg
    81961 tttgtctggt cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac
    82021 tccttaaaat tgtataccat acaattttac cattgattgt attgatttta gaagttaatc
    82081 ttttgtgagg agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt
    82141 tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa
    82201 aatcagccaa aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt
    82261 tgcgatgatg cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc
    82321 ttttccggat aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga
    82381 tgctcaaaac ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac
    82441 agctgctgta tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc
    82501 tttaattgga gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc
    82561 catcactaca gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa
    82621 agctaaaaaa cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt
    82681 acgtccagcg gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga
    82741 gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga
    82801 gataagtaca tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt
    82861 aacagaaagc ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat
    82921 tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag
    82981 gccgacgatt ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat
    83041 ttataacaaa ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca
    83101 tttgcagatg aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg
    83161 ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca
    83221 gtgaagaaaa accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt
    83281 tgtggtcacg cgacattagc aacagcattt gttttattta actattacaa aatacctgat
    83341 gaaacgatta aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat
    83401 tattacatgg attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa
    83461 gaagcgattg gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta
    83521 gaagatgaaa aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa
    83581 ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc
    83641 ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt
    83701 attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca
    83761 aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca
    83821 gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat
    83881 tggtggagag tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc
    83941 aatttaatga aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa
    84001 gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac catcagggtc
    84061 aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag
    84121 accagcagct ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg
    84181 accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc
    84241 atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg
    84301 gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc
    84361 tttcactcga acacatgtgt gagccatttt catagattca tctctccttt tccttttact
    84421 caatcataac aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct
    84481 catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat
    84541 ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat
    84601 agaaagagat cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg
    84661 cggggaaatt gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga
    84721 aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc
    84781 gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa
    84841 gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc
    84901 gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa
    84961 taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta
    85021 catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg
    85081 ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa
    85141 tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa
    85201 ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga
    85261 agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg
    85321 caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt
    85381 ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt
    85441 ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat
    85501 ggagatgact gccgacacgg cgtgccatta cttcgatctc taggaaatct tcatgtgtca
    85561 gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag
    85621 taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg
    85681 gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg
    85741 cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga
    85801 tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg
    85861 cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat
    85921 cacgcaagga cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt
    85981 gtcccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat
    86041 ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag
    86101 gtagatcgta gctagaattc acagcagtgt aataaacagg attatatccg accttttcta
    86161 ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt
    86221 tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt
    86281 cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta
    86341 aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg
    86401 catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac
    86461 cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta acgatataat
    86521 cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca
    86581 cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc
    86641 gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg
    86701 ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc
    86761 ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt
    86821 catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat
    86881 gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta
    86941 ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa
    87001 cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc
    87061 ctaattgttt aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta
    87121 cttgatgctg gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca
    87181 gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc
    87241 attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta
    87301 gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg
    87361 acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga
    87421 tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc
    87481 ttcatgactt gcccacgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa
    87541 atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa
    87601 tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga
    87661 aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt
    87721 gtgtcacttt gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct
    87781 aatccacctg aatttctgat cactggagcg taacctgcat cttccagtgt ttggatcccg
    87841 tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc
    87901 cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat
    87961 gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc
    88021 attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa
    88081 gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag
    88141 aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag
    88201 gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt
    88261 tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata
    88321 aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg
    88381 gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag
    88441 aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga
    88501 aagaactgca tgttagtttg aaagaccgtc ccacagcagg gaaggttcga atcgattatg
    88561 atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt
    88621 aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt
    88681 gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat
    88741 caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg
    88801 atttagctaa ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac
    88861 tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc
    88921 gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa
    88981 tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta
    89041 tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga
    89101 cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg
    89161 cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact
    89221 tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt
    89281 atgaagagga agaattttct gactctgaaa aagaataaac attattttct tacgagaggt
    89341 tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacgatttt tgtcacacct
    89401 tcttttttgt atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt
    89461 agcgcacctt gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg
    89521 atataagaaa aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac
    89581 tgattatcaa aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt
    89641 agatgtgatg caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat
    89701 cgagcatttt agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg
    89761 cagacaaaaa aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc
    89821 tttagaaact tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa
    89881 agagattctg atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt
    89941 ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca
    90001 aatcctagaa tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac
    90061 taaagaaaac ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc
    90121 gttttattat cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag
    90181 ggttcggagt atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc
    90241 aagttcgaag ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag
    90301 gcgaacgctt tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac
    90361 aaaatgagaa agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac
    90421 aagcagattt tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag
    90481 ttttgggaga ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga
    90541 tcacccaaac agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa
    90601 acagtgtcca gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga
    90661 caaaagaagt caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt
    90721 ggactgagca agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac
    90781 gaagttttta tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg
    90841 ctcctggtta tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt
    90901 tggcatcgga agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat
    90961 tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa
    91021 aagaaattat ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac
    91081 ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc
    91141 acaatctttc aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa
    91201 cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac
    91261 acaagacagt acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga
    91321 tgatggacca ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt
    91381 gcatgccact tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca
    91441 gacagctgaa gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac
    91501 tctttctgcc cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt
    91561 cactggcaaa acccccactt ttgttcgtcc gccctatggt tctgtcacga atgtaggagc
    91621 aacgatcatc cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag
    91681 aaatcctgat ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt
    91741 attccatgat atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa
    91801 agagcaaggg tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga
    91861 gaactattat ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat
    91921 aaataacaca ttttaataag tagttgaaca cataactacc ttacttgcaa aggcttaaca
    91981 atatctatac aggtacaaca aaaaactgtc caccaatt
//
LOCUS       DANKHJ010000004        78790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000004 DANKHJ010000000
VERSION     DANKHJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..434)
                     /locus_tag="PW113_000335"
     CDS             complement(<1..434)
                     /locus_tag="PW113_000335"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2524676.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
     gene            680..1627
                     /locus_tag="PW113_000336"
     CDS             680..1627
                     /locus_tag="PW113_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2524677.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(1838..3910)
                     /locus_tag="PW113_000337"
     CDS             complement(1838..3910)
                     /locus_tag="PW113_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2524678.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(3843..4220)
                     /locus_tag="PW113_000338"
     CDS             complement(3843..4220)
                     /locus_tag="PW113_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2524679.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(4349..4927)
                     /locus_tag="PW113_000339"
     CDS             complement(4349..4927)
                     /locus_tag="PW113_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2524680.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            5059..6351
                     /locus_tag="PW113_000340"
     CDS             5059..6351
                     /locus_tag="PW113_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2524681.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(6411..7529)
                     /locus_tag="PW113_000341"
     CDS             complement(6411..7529)
                     /locus_tag="PW113_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2524682.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(7543..8964)
                     /gene="arcD"
                     /locus_tag="PW113_000342"
     CDS             complement(7543..8964)
                     /gene="arcD"
                     /locus_tag="PW113_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2524683.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(9210..10331)
                     /gene="mnmA"
                     /locus_tag="PW113_000343"
     CDS             complement(9210..10331)
                     /gene="mnmA"
                     /locus_tag="PW113_000343"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2524684.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            10735..11334
                     /locus_tag="PW113_000344"
     CDS             10735..11334
                     /locus_tag="PW113_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2524685.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(11472..12758)
                     /gene="efmA"
                     /locus_tag="PW113_000345"
     CDS             complement(11472..12758)
                     /gene="efmA"
                     /locus_tag="PW113_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2524686.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(13029..13373)
                     /locus_tag="PW113_000346"
     CDS             complement(13029..13373)
                     /locus_tag="PW113_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2524687.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(13510..14655)
                     /locus_tag="PW113_000347"
     CDS             complement(13510..14655)
                     /locus_tag="PW113_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2524688.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(14835..15809)
                     /locus_tag="PW113_000348"
     CDS             complement(14835..15809)
                     /locus_tag="PW113_000348"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2524689.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(15889..16764)
                     /locus_tag="PW113_000349"
     CDS             complement(15889..16764)
                     /locus_tag="PW113_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2524690.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            16894..18801
                     /locus_tag="PW113_000350"
     CDS             16894..18801
                     /locus_tag="PW113_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2524691.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(19342..22620)
                     /locus_tag="PW113_000351"
     CDS             complement(19342..22620)
                     /locus_tag="PW113_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2524692.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(22891..23436)
                     /locus_tag="PW113_000352"
     CDS             complement(22891..23436)
                     /locus_tag="PW113_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2524693.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(23448..24140)
                     /locus_tag="PW113_000353"
     CDS             complement(23448..24140)
                     /locus_tag="PW113_000353"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2524694.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(24159..24443)
                     /locus_tag="PW113_000354"
     CDS             complement(24159..24443)
                     /locus_tag="PW113_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524695.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(24456..25019)
                     /locus_tag="PW113_000355"
     CDS             complement(24456..25019)
                     /locus_tag="PW113_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2524696.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            25247..25909
                     /locus_tag="PW113_000356"
     CDS             25247..25909
                     /locus_tag="PW113_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2524697.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            26033..27229
                     /locus_tag="PW113_000357"
     CDS             26033..27229
                     /locus_tag="PW113_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2524698.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(27330..28706)
                     /locus_tag="PW113_000358"
     CDS             complement(27330..28706)
                     /locus_tag="PW113_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2524699.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(28743..29610)
                     /locus_tag="PW113_000359"
                     /pseudo
     CDS             complement(28743..29610)
                     /locus_tag="PW113_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(29699..30724)
                     /locus_tag="PW113_000360"
     CDS             complement(29699..30724)
                     /locus_tag="PW113_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2524700.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(30742..31416)
                     /locus_tag="PW113_000361"
     CDS             complement(30742..31416)
                     /locus_tag="PW113_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2524701.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(31600..32493)
                     /locus_tag="PW113_000362"
     CDS             complement(31600..32493)
                     /locus_tag="PW113_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2524702.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(32567..34264)
                     /locus_tag="PW113_000363"
     CDS             complement(32567..34264)
                     /locus_tag="PW113_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2524703.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(34257..35570)
                     /locus_tag="PW113_000364"
     CDS             complement(34257..35570)
                     /locus_tag="PW113_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2524704.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(35570..39607)
                     /locus_tag="PW113_000365"
     CDS             complement(35570..39607)
                     /locus_tag="PW113_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2524705.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(39620..40078)
                     /locus_tag="PW113_000366"
     CDS             complement(39620..40078)
                     /locus_tag="PW113_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2524706.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(40376..40894)
                     /locus_tag="PW113_000367"
     CDS             complement(40376..40894)
                     /locus_tag="PW113_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2524707.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(40896..41513)
                     /locus_tag="PW113_000368"
     CDS             complement(40896..41513)
                     /locus_tag="PW113_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2524708.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(41510..42364)
                     /locus_tag="PW113_000369"
     CDS             complement(41510..42364)
                     /locus_tag="PW113_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2524709.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(42361..43272)
                     /locus_tag="PW113_000370"
     CDS             complement(42361..43272)
                     /locus_tag="PW113_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2524710.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(43313..44647)
                     /locus_tag="PW113_000371"
     CDS             complement(43313..44647)
                     /locus_tag="PW113_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2524711.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            44855..45685
                     /locus_tag="PW113_000372"
     CDS             44855..45685
                     /locus_tag="PW113_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2524712.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(45929..46247)
                     /locus_tag="PW113_000373"
                     /pseudo
     CDS             complement(45929..46247)
                     /locus_tag="PW113_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(46369..46563)
                     /locus_tag="PW113_000374"
     CDS             complement(46369..46563)
                     /locus_tag="PW113_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524713.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(47068..47253)
                     /locus_tag="PW113_000375"
     CDS             complement(47068..47253)
                     /locus_tag="PW113_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524714.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(48103..48444)
                     /locus_tag="PW113_000376"
     CDS             complement(48103..48444)
                     /locus_tag="PW113_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2524715.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(48444..48674)
                     /locus_tag="PW113_000377"
     CDS             complement(48444..48674)
                     /locus_tag="PW113_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2524716.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            48961..49299
                     /locus_tag="PW113_000378"
     CDS             48961..49299
                     /locus_tag="PW113_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524717.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(49504..49743)
                     /locus_tag="PW113_000379"
     CDS             complement(49504..49743)
                     /locus_tag="PW113_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2524718.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(49719..50066)
                     /locus_tag="PW113_000380"
     CDS             complement(49719..50066)
                     /locus_tag="PW113_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2524719.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(50798..51379)
                     /locus_tag="PW113_000381"
     CDS             complement(50798..51379)
                     /locus_tag="PW113_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2524720.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(51562..52188)
                     /locus_tag="PW113_000382"
     CDS             complement(51562..52188)
                     /locus_tag="PW113_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2524721.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(52210..53541)
                     /locus_tag="PW113_000383"
     CDS             complement(52210..53541)
                     /locus_tag="PW113_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2524722.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(53696..54097)
                     /locus_tag="PW113_000384"
     CDS             complement(53696..54097)
                     /locus_tag="PW113_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2524723.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(54183..54389)
                     /locus_tag="PW113_000385"
     CDS             complement(54183..54389)
                     /locus_tag="PW113_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524724.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(54484..55986)
                     /locus_tag="PW113_000386"
     CDS             complement(54484..55986)
                     /locus_tag="PW113_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2524725.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(56004..56459)
                     /gene="argR"
                     /locus_tag="PW113_000387"
     CDS             complement(56004..56459)
                     /gene="argR"
                     /locus_tag="PW113_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2524726.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(56557..57174)
                     /locus_tag="PW113_000388"
     CDS             complement(56557..57174)
                     /locus_tag="PW113_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2524727.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            57395..58768
                     /locus_tag="PW113_000389"
     CDS             57395..58768
                     /locus_tag="PW113_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2524728.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            58843..59955
                     /locus_tag="PW113_000390"
     CDS             58843..59955
                     /locus_tag="PW113_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2524729.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(60027..61664)
                     /locus_tag="PW113_000391"
     CDS             complement(60027..61664)
                     /locus_tag="PW113_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2524730.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(61832..62440)
                     /locus_tag="PW113_000392"
     CDS             complement(61832..62440)
                     /locus_tag="PW113_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2524731.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(62824..63975)
                     /gene="queG"
                     /locus_tag="PW113_000393"
     CDS             complement(62824..63975)
                     /gene="queG"
                     /locus_tag="PW113_000393"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2524732.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(63994..64746)
                     /locus_tag="PW113_000394"
     CDS             complement(63994..64746)
                     /locus_tag="PW113_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524733.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(64887..65836)
                     /locus_tag="PW113_000395"
                     /pseudo
     CDS             complement(64887..65836)
                     /locus_tag="PW113_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(65837..66676)
                     /locus_tag="PW113_000396"
     CDS             complement(65837..66676)
                     /locus_tag="PW113_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2524734.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(66673..67419)
                     /locus_tag="PW113_000397"
     CDS             complement(66673..67419)
                     /locus_tag="PW113_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2524735.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            67657..69027
                     /locus_tag="PW113_000398"
     CDS             67657..69027
                     /locus_tag="PW113_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2524736.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(69082..69978)
                     /locus_tag="PW113_000399"
     CDS             complement(69082..69978)
                     /locus_tag="PW113_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2524737.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(70092..70262)
                     /locus_tag="PW113_000400"
     CDS             complement(70092..70262)
                     /locus_tag="PW113_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524738.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(70259..70936)
                     /locus_tag="PW113_000401"
     CDS             complement(70259..70936)
                     /locus_tag="PW113_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2524739.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(71027..71932)
                     /locus_tag="PW113_000402"
     CDS             complement(71027..71932)
                     /locus_tag="PW113_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2524740.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            72107..73531
                     /locus_tag="PW113_000403"
     CDS             72107..73531
                     /locus_tag="PW113_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2524741.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            73577..74608
                     /locus_tag="PW113_000404"
     CDS             73577..74608
                     /locus_tag="PW113_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2524742.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            74615..75802
                     /locus_tag="PW113_000405"
     CDS             74615..75802
                     /locus_tag="PW113_000405"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2524743.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            75958..77340
                     /locus_tag="PW113_000406"
     CDS             75958..77340
                     /locus_tag="PW113_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2524744.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            77467..77697
                     /locus_tag="PW113_000407"
     CDS             77467..77697
                     /locus_tag="PW113_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524745.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(77897..78565)
                     /locus_tag="PW113_000408"
     CDS             complement(77897..78565)
                     /locus_tag="PW113_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2524746.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        22555 a        16518 c        14074 g        25643 t
ORIGIN      
        1 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
       61 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
      121 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
      181 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
      241 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
      301 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
      361 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
      421 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
      481 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
      541 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
      601 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
      661 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
      721 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
      781 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
      841 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
      901 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
      961 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     1021 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     1081 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     1141 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     1201 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     1261 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     1321 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     1381 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     1441 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     1501 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     1561 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     1621 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     1681 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     1741 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     1801 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     1861 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     1921 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     1981 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     2041 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     2101 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     2161 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     2221 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     2281 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     2341 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     2401 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     2461 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     2521 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     2581 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     2641 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     2701 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     2761 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     2821 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     2881 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     2941 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     3001 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     3061 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     3121 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     3181 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     3241 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     3301 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     3361 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     3421 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     3481 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     3541 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     3601 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     3661 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     3721 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     3781 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     3841 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     3901 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     3961 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     4021 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     4081 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     4141 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     4201 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     4261 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     4321 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     4381 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     4441 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     4501 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     4561 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     4621 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     4681 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     4741 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     4801 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     4861 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     4921 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     4981 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     5041 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     5101 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     5161 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     5221 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     5281 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     5341 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     5401 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     5461 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     5521 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     5581 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     5641 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     5701 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     5761 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     5821 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     5881 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     5941 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     6001 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     6061 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     6121 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     6181 tccagtgcat ttggttatcg cggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     6241 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     6301 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     6361 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     6421 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     6481 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     6541 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     6601 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     6661 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     6721 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     6781 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     6841 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     6901 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     6961 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     7021 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     7081 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     7141 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     7201 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     7261 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     7321 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     7381 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     7441 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     7501 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     7561 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     7621 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     7681 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     7741 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     7801 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     7861 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     7921 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     7981 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     8041 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     8101 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     8161 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     8221 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     8281 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     8341 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     8401 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     8461 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     8521 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     8581 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     8641 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     8701 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
     8761 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
     8821 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
     8881 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
     8941 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
     9001 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
     9061 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
     9121 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
     9181 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
     9241 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
     9301 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
     9361 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
     9421 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
     9481 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
     9541 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
     9601 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
     9661 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
     9721 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
     9781 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
     9841 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
     9901 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
     9961 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    10021 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    10081 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    10141 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    10201 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    10261 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    10321 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    10381 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    10441 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    10501 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    10561 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    10621 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    10681 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    10741 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    10801 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    10861 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    10921 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    10981 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    11041 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    11101 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    11161 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    11221 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    11281 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    11341 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    11401 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    11461 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    11521 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    11581 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    11641 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    11701 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    11761 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    11821 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    11881 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    11941 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    12001 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    12061 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    12121 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    12181 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    12241 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    12301 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    12361 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    12421 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    12481 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    12541 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    12601 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    12661 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    12721 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    12781 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    12841 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    12901 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    12961 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    13021 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    13081 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    13141 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    13201 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    13261 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    13321 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    13381 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    13441 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    13501 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    13561 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    13621 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    13681 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    13741 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    13801 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    13861 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    13921 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    13981 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    14041 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    14101 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    14161 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    14221 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    14281 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    14341 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    14401 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    14461 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    14521 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    14581 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    14641 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    14701 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    14761 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    14821 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    14881 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    14941 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    15001 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    15061 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    15121 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    15181 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    15241 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    15301 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    15361 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    15421 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    15481 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    15541 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    15601 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    15661 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    15721 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    15781 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    15841 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    15901 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    15961 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    16021 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    16081 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    16141 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    16201 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    16261 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    16321 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    16381 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    16441 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    16501 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    16561 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    16621 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    16681 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    16741 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    16801 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    16861 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    16921 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    16981 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    17041 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    17101 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    17161 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    17221 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    17281 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    17341 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    17401 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    17461 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    17521 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    17581 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    17641 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    17701 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    17761 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    17821 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    17881 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    17941 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    18001 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    18061 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    18121 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    18181 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    18241 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    18301 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    18361 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    18421 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    18481 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    18541 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    18601 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    18661 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    18721 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    18781 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    18841 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    18901 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    18961 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    19021 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    19081 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    19141 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    19201 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    19261 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    19321 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    19381 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    19441 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    19501 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    19561 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    19621 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    19681 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    19741 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    19801 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    19861 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    19921 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    19981 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    20041 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    20101 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    20161 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    20221 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    20281 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    20341 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    20401 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    20461 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    20521 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    20581 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    20641 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    20701 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    20761 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    20821 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    20881 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    20941 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    21001 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    21061 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    21121 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    21181 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    21241 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    21301 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    21361 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    21421 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    21481 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    21541 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    21601 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    21661 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    21721 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    21781 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    21841 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    21901 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    21961 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    22021 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    22081 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    22141 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    22201 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    22261 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    22321 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    22381 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    22441 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    22501 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    22561 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    22621 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    22681 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    22741 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    22801 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    22861 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    22921 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa
    22981 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    23041 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    23101 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    23161 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    23221 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    23281 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    23341 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    23401 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    23461 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    23521 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    23581 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    23641 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    23701 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    23761 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    23821 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    23881 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    23941 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    24001 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    24061 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    24121 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    24181 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    24241 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    24301 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    24361 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    24421 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    24481 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    24541 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    24601 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    24661 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    24721 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    24781 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    24841 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    24901 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    24961 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    25021 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    25081 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    25141 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    25201 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    25261 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    25321 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    25381 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    25441 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    25501 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    25561 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    25621 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    25681 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    25741 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    25801 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    25861 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    25921 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    25981 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    26041 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    26101 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    26161 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    26221 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    26281 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    26341 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    26401 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    26461 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    26521 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    26581 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    26641 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    26701 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    26761 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    26821 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    26881 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    26941 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    27001 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    27061 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    27121 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    27181 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    27241 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    27301 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    27361 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    27421 acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat tggcaatagc
    27481 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    27541 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    27601 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    27661 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    27721 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    27781 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    27841 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    27901 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    27961 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    28021 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    28081 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    28141 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    28201 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    28261 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    28321 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    28381 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    28441 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    28501 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    28561 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    28621 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    28681 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    28741 gattaaactg ttttcatata ccctaaaagg gataccacct aaaatgacta aaaggcaacc
    28801 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
    28861 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
    28921 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
    28981 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
    29041 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
    29101 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
    29161 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
    29221 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
    29281 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
    29341 caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
    29401 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
    29461 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
    29521 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
    29581 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
    29641 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
    29701 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
    29761 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
    29821 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
    29881 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
    29941 ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
    30001 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
    30061 aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg
    30121 cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt
    30181 cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
    30241 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
    30301 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
    30361 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
    30421 tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat
    30481 aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
    30541 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
    30601 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
    30661 caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
    30721 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
    30781 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
    30841 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
    30901 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
    30961 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
    31021 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
    31081 gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat
    31141 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
    31201 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
    31261 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
    31321 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
    31381 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
    31441 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
    31501 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag
    31561 cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg
    31621 ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa
    31681 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
    31741 tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt
    31801 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa
    31861 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
    31921 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
    31981 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
    32041 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
    32101 atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg
    32161 ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt
    32221 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
    32281 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
    32341 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
    32401 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
    32461 ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac
    32521 tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact
    32581 tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
    32641 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
    32701 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
    32761 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg
    32821 ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat
    32881 ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc
    32941 ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt
    33001 cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt
    33061 tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat
    33121 caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt
    33181 ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg
    33241 ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga
    33301 caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc
    33361 cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga
    33421 ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa
    33481 taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg
    33541 tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat
    33601 tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt
    33661 atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg
    33721 gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta
    33781 agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga
    33841 ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta
    33901 aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac
    33961 cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta
    34021 cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca
    34081 atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg
    34141 tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa
    34201 tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat
    34261 tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt
    34321 cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct
    34381 gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg
    34441 gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat
    34501 tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc
    34561 acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct
    34621 tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc
    34681 gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca
    34741 ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa
    34801 tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct
    34861 ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg
    34921 acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa
    34981 attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact
    35041 tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta
    35101 ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt
    35161 accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg
    35221 ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt
    35281 tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc
    35341 tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca
    35401 tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat
    35461 tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca
    35521 agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc
    35581 cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga
    35641 atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt
    35701 caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt
    35761 ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa
    35821 cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt
    35881 ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact
    35941 tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat agggaagaat
    36001 cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt
    36061 tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa
    36121 gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga
    36181 tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa
    36241 acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg
    36301 gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat
    36361 ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca
    36421 aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg
    36481 aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt
    36541 ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc
    36601 ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt
    36661 gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt
    36721 catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt
    36781 tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa
    36841 ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc
    36901 tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga
    36961 tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat
    37021 tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt
    37081 ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag
    37141 aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga
    37201 ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt
    37261 tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg
    37321 gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa
    37381 tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg
    37441 gttcggcttc cccactctct ggattttggt catataatgc aaattcgact ccagccaacg
    37501 gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa
    37561 tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt
    37621 tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa
    37681 gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt
    37741 tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg
    37801 cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc
    37861 ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat
    37921 taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga
    37981 tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta
    38041 cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc
    38101 gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt
    38161 tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact
    38221 tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt
    38281 gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat
    38341 cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag
    38401 tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat
    38461 cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa
    38521 tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat
    38581 ttctattttc tccgccattg tctttataca atgtttcata gccattttta agccctagtg
    38641 taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac
    38701 gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag
    38761 ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga
    38821 gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat
    38881 tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga
    38941 ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg
    39001 ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt
    39061 ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc
    39121 aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat
    39181 tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga
    39241 tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg
    39301 tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt
    39361 cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc
    39421 caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc
    39481 tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag
    39541 cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa
    39601 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    39661 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    39721 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    39781 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    39841 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    39901 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    39961 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    40021 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    40081 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    40141 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    40201 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    40261 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    40321 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    40381 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    40441 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    40501 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    40561 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    40621 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    40681 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    40741 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    40801 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    40861 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    40921 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    40981 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    41041 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    41101 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    41161 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    41221 atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    41281 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    41341 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    41401 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    41461 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    41521 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    41581 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    41641 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    41701 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    41761 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    41821 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    41881 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    41941 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    42001 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    42061 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    42121 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    42181 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    42241 atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga
    42301 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    42361 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    42421 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    42481 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    42541 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    42601 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    42661 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    42721 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    42781 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    42841 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    42901 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    42961 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    43021 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    43081 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    43141 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    43201 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    43261 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    43321 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    43381 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    43441 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    43501 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    43561 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    43621 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    43681 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    43741 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    43801 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    43861 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    43921 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    43981 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    44041 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    44101 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    44161 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    44221 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    44281 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    44341 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    44401 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    44461 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    44521 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    44581 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    44641 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    44701 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    44761 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    44821 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    44881 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    44941 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    45001 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    45061 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    45121 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    45181 gcacgcgatc gtagatggct taggacctcg tcctgctgac agcgtgggca atccatggag
    45241 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    45301 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    45361 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    45421 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    45481 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    45541 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    45601 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    45661 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    45721 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    45781 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    45841 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    45901 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    45961 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    46021 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    46081 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    46141 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    46201 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    46261 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    46321 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    46381 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    46441 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    46501 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    46561 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    46621 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    46681 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    46741 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    46801 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    46861 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    46921 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    46981 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    47041 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    47101 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    47161 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    47221 gatggttagg aatcctttag aatttttact cattcataga cacttcctta aataactttt
    47281 attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaacc
    47341 ttacattata ctggaatgaa ctgtattgat agtgtaccta aaagctctac tgacataaga
    47401 tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat gtaatcaatt agagaataaa
    47461 ctaatcataa aaggtacttt tacattcata attgtcgctt tgagattcta ctttggttca
    47521 ctatctgacc caattgcaca ttttcgatca ttacaagtat tggtgtcgtt agaacaacta
    47581 aataaaaact agcggtagat ttttcagaat ctatgctaat ttctgtatgg atgttccagt
    47641 gatggcaaat ttctcttttc attgtttttc ttgtcgcatg atatgccttt ttgtgttatt
    47701 cagttaaact atagaaattc tttcctagac agatcttgat cccaaatttt acgtttttga
    47761 gcatcgatat agtttaaaat gacattactc atatctaaag agtttagatt ttttaggact
    47821 aaaactgtca gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata
    47881 cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat gacttgagga
    47941 aatcacctca atcattcgtt taaaatgcta tatattcttt agtttaagat acggaaatta
    48001 atgccatcga ccacactagt agaatgaacg acatttactt ggcctttttt taatttcgtt
    48061 atgtttagca tataaaaata ttatagagtt tacttacaat gcttaaaaca tatactgtat
    48121 aatctgatca attttagcca tgtcttgcaa tggtaagttt tctatttttt tgagttttcg
    48181 ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta tatctagctc
    48241 ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg gacatatgac
    48301 cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg gtcgtctttt
    48361 ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac cttttttagg
    48421 aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc
    48481 cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt
    48541 ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa
    48601 ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat
    48661 tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac actaagtaac
    48721 gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg
    48781 atataaatag aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa
    48841 gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc
    48901 ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc
    48961 atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga
    49021 aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt
    49081 caaatagaaa cattggttca acgaatttca actagacaag actttgctat tgtagaacgt
    49141 gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg
    49201 gataaatggc aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta
    49261 actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata
    49321 aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg
    49381 actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt
    49441 tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt
    49501 atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa
    49561 agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc
    49621 atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt
    49681 ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa
    49741 cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat
    49801 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
    49861 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
    49921 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
    49981 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
    50041 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
    50101 ctgcaacaag aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg
    50161 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
    50221 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
    50281 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
    50341 ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac
    50401 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50461 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
    50521 atgcagggca gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg
    50581 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    50641 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
    50701 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    50761 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
    50821 acaatctttc tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
    50881 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
    50941 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
    51001 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
    51061 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
    51121 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
    51181 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
    51241 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
    51301 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
    51361 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
    51421 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
    51481 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
    51541 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
    51601 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
    51661 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
    51721 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
    51781 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
    51841 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
    51901 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
    51961 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
    52021 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
    52081 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
    52141 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
    52201 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
    52261 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
    52321 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
    52381 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
    52441 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
    52501 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
    52561 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
    52621 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
    52681 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
    52741 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
    52801 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
    52861 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
    52921 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
    52981 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
    53041 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
    53101 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
    53161 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
    53221 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
    53281 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
    53341 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
    53401 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
    53461 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
    53521 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
    53581 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
    53641 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
    53701 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
    53761 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta
    53821 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
    53881 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
    53941 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
    54001 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
    54061 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
    54121 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
    54181 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
    54241 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
    54301 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
    54361 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
    54421 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
    54481 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
    54541 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
    54601 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
    54661 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
    54721 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
    54781 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
    54841 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
    54901 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
    54961 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
    55021 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
    55081 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
    55141 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
    55201 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
    55261 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
    55321 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
    55381 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
    55441 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
    55501 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
    55561 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
    55621 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
    55681 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
    55741 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
    55801 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
    55861 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
    55921 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
    55981 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
    56041 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
    56101 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
    56161 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
    56221 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
    56281 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
    56341 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
    56401 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
    56461 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
    56521 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
    56581 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
    56641 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
    56701 cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
    56761 catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat
    56821 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
    56881 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
    56941 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
    57001 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
    57061 attcgggtcg ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg
    57121 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
    57181 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
    57241 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
    57301 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
    57361 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
    57421 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
    57481 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
    57541 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
    57601 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
    57661 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
    57721 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
    57781 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
    57841 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
    57901 gtttcttgtg cccgttttgt ttctttcttt gattatcggc tggtttgcta ttcccgagat
    57961 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
    58021 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
    58081 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
    58141 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
    58201 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
    58261 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
    58321 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
    58381 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    58441 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    58501 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
    58561 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    58621 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    58681 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    58741 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    58801 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    58861 atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat
    58921 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    58981 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    59041 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    59101 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    59161 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    59221 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    59281 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    59341 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    59401 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    59461 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    59521 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    59581 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    59641 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    59701 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    59761 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    59821 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    59881 ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg
    59941 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    60001 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    60061 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    60121 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    60181 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    60241 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    60301 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    60361 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    60421 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    60481 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    60541 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    60601 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    60661 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    60721 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    60781 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    60841 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    60901 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    60961 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    61021 tctccaaagt aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg
    61081 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    61141 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    61201 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    61261 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    61321 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    61381 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    61441 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    61501 atcgttcctg gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga
    61561 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    61621 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    61681 ctaaacaaaa aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg
    61741 ctgtgggaag caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt
    61801 ttctcggttt catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg
    61861 ttttagcagc atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt
    61921 gattggcagc tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt
    61981 aacgtccgtt agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg
    62041 cagagcttga agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt
    62101 caacaggtgc aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt
    62161 ctgttgtttt gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt
    62221 caccaacata aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca
    62281 ttgagttgtc tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg
    62341 tataaacttc atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag
    62401 caaaagttgt acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc
    62461 cgttttttat tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag
    62521 aaacatgaca tttaatgaca aaataacgaa ttatttaata tttgtaacat ttaaacaaat
    62581 atttttttga tacaaaacca taaaagcctt gttgtataag caaagtaatc gtttttctat
    62641 gtttttttat ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa
    62701 aaaaaagcaa aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca
    62761 aaaaaaccag taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt
    62821 tttttatttt aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac
    62881 ttcttctgtt tctttagcta atatttcatc taatacatca agccattttt ccggttcttt
    62941 tgttcctaga cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt
    63001 taaacattct gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa
    63061 tgcgtttctc tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg
    63121 tttgaagtct ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac
    63181 ttttggttcc atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa
    63241 ctggcagata tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat
    63301 catccctttt gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc
    63361 aagcaatgct tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc
    63421 tcctggttta tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga
    63481 accgaactct tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg
    63541 tgctacagcg acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc
    63601 ttctttttgc catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc
    63661 tctgagtata tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc
    63721 atccctaggc acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga
    63781 tttcggctga tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa
    63841 gccagaagta tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg
    63901 ttccgccgtg gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc
    63961 ttcttttaat gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag
    64021 tttttctttt ttcagctgga caaactggat ctgctgatcg gcaatcttct tttcttccat
    64081 ccattttttt cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt
    64141 atctaatgca ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct
    64201 tacctcttcc catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag
    64261 atttttttgc tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac
    64321 tagacgatta taaaacatat tcattaattt gctgttggca ataaattgac ttttataata
    64381 ggccgactgg atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca
    64441 tgctttgacg gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg
    64501 agaaaggaac acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa
    64561 caaccatttg cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat
    64621 gaataattta gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc
    64681 ctgctggctg atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa
    64741 attcatagcc acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta
    64801 tctgtacgac gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct
    64861 gctcctcaag ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg
    64921 atcgtttcaa tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt
    64981 gctatggaat cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt
    65041 tccatgcttc ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt
    65101 atctgatcaa tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc
    65161 catatataag ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt
    65221 gttactaata attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct
    65281 aattctttta gtttttccac atatgcttct gcattttttt atagagtgtc gcattttctg
    65341 catctttttc agagaatata cgtgcgattt cctccacata ttttattccg ttagataggt
    65401 ctagccaagc atggggatct gcttttgtat gttcttcacc gctagttaaa taaagaggtt
    65461 ctacattttt gctaactgca aaataatctt ttccttcttc ttttttagcc gtttccatca
    65521 agttatcgaa ccagctgtta cctgtttcaa gattcaaacc gttgtataaa ataacatctg
    65581 catcgcttgc ctttttgatg tcttctggta gtacttcata ttcatgcgga tctgttccga
    65641 ctggtacgat actgtggata tctatttgat ctgttccgac ctcttttgcc atgtccgcca
    65701 agatcgaatt ggtagccacg actgacaact tttctttact tccttcatcc gcttggtcag
    65761 aagcctgctt tccgcaaccg gctaatacca agaaactaac tgccaagatt aaaaagagtg
    65821 atttttttgt tttcatctat ctgcttcctt tctgaaataa aattcctttt gttggagaaa
    65881 ataaaaaagc aaggataaaa aatgcggtac atgctaacac gatcgtagct cctgatggcc
    65941 aattgtaact ataactgaaa aacacaccga caactgcact tagcataccg attcctgcag
    66001 ctgtaatgat cattttcagt aaatggttcg ttagcaagta agccgtggcg gctggtgtga
    66061 tcaacatggc aatcaccaag attgttccta ctgtctgtaa actgactaca gccactaatg
    66121 tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt cagcccataa gcttttgcca
    66181 ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa gaaaaagaca aatatcaaga
    66241 cgattccact aacgagtgct gtcagcaaca aatcgctttc cctaacagct aagacattcc
    66301 caaataaaat atgatacaaa tctgtcgcac tctgggcata tgaaatcaag ataatcccta
    66361 atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc atttttcaat cggctccgct
    66421 gagtcacgaa accaatcact gctgctgtta gcatcccaaa agcagtggct ccaaagatgt
    66481 aactaaaacc aaacatataa gaaatggcta cacctggaag aactgcgtga gagatagcgt
    66541 ctcccatcaa agacatccct cgtaaaataa taaaagaacc gatgactcca gaagcaagtc
    66601 ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg ataattcatt aatccatcaa
    66661 taaaggattg gatcattttt tctctcctcc ttttactaaa tgtctgatga tctcaccata
    66721 ggccaattga aggttcttcg atgtaaagac ttcttgtact ggaccggaag cgatcagctg
    66781 tttatttaaa agaataacct catcgaaata ttcttctact ttatgaagat catgatggac
    66841 gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc
    66901 acttaacgca tcaatcccta cgaatggttc atctaaaaag atccattctg ctccttgggc
    66961 tagagctctt gcaataaaaa ctctctgaag ctgtccaccc gatagttcgc tgatctgtct
    67021 tttggcatat tcttccatcc ctactttttt caaagcttgt cttgcacgtt ctttttcttt
    67081 tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa
    67141 tacatcaatt ggaaaggaaa gatccaattc acttcgctgt tccacgtagg ctattttttt
    67201 acggactgag tttaccgact gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa
    67261 ctctagcaaa gatttcataa atgttgattt tcctgcaccg tttggtccga taataccggt
    67321 aattttttgt ggatttatcg tcaagttgat agttttcaaa acagttttcc cttgataagc
    67381 ggctgaaagg tcttgaatta caatggccgt acgttccaaa tcttacactc ctttttaggt
    67441 ttacctaact ttttatttaa atatagttta actaaaatgg aaagcatttt caagtagaat
    67501 gatgaaaaag tttttttcga attttcctcg cattttatga aaattttctg tagaatcaca
    67561 tcaagagttc aataaggcta acctaataat gaaaaacatg tgttgaaacg tttgattatt
    67621 ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga
    67681 agtcttagtg cccgtcatat ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg
    67741 tttatgggtg cctcctcaac catacgttgg acagggccct cagtgatggg agcctatgcg
    67801 attgcaggat tctttttgta tttgattatg cgtgctttag gcgaaatgct ttacgttgat
    67861 ccttcgactg gttcttttgc aaattacgcg agcgagtaca tccatcctgt ggctggctac
    67921 ttgaccgctt ggagtaatat ttttcaatat attgtcgtag gaataagtga agtgattgct
    67981 gtaggatctt atatgaatta ttggtggcct gatttgccgg caattattcc cggaatcatt
    68041 gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca aggcgtttgg cgaattagaa
    68101 ttttggtttt cgatcatcaa agtcataacg atcattttga tgatcattgc aggtcttggg
    68161 gtaatcttat ttggatttgg taatcatggt caggcagtag ggatttcaaa tctttggaaa
    68221 aatggtggat tcttcactgg aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt
    68281 gcttcctatc aaggaatcga actgatcgga atgactgctg gtgaagcaga gaatcctaaa
    68341 aagacgatca ttgaagctgt ccagtccact atcggtcgaa tcttgatttt ttatattggt
    68401 gctattttta tcattgtgag tatctatccg tggaatcaat tgagtgagat tggttcacct
    68461 tttgtacaaa ctttttctaa agtcggcatt acatttgctg caggactgat taattttgtc
    68521 gtgattacag cggcattatc aggttgcaat tcagggattt tcagcgcaag tcgtatggtc
    68581 tatacacttg ccataaatgg aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt
    68641 gcgccttttt atcctgtcgt agcaatctca ggtggaatct taattggtct gatcttaaat
    68701 tatcttctac caatgttcat caaccagtca gaaaatattt tcgttttcgt atacagctca
    68761 agtattttgc ctggtatggt tccttgggta gtgattttga tcagcgagat tcgcttcaaa
    68821 aagaagcacg cagataaaat gcatgatcat ccttttaaaa tgccactatc tccttatacg
    68881 aattatgtga cacttgcttt tttagctatc gtattgattt tcatgtttat caatcctgaa
    68941 acgagaatct cattgattat tggactcgtt ttcttgcttt acatgacgat ccattacttt
    69001 gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag cttcacctta gtggaagctt
    69061 cgttcttttt tattgctttt atcaattcaa ttttagcgtc tcaccgggag tcaggaaata
    69121 attctccatg ctcatcccat tcagcgacaa gatcgtttct actggtacgc cagttcgttc
    69181 ggctatttga tttggacctt ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc
    69241 gctgttggta ttagtttcag aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc
    69301 agaagcttct accgactctg ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt
    69361 tgactctgct tgaagagagg cgctagtctg caaataaata tcggaaatct tagcgacatg
    69421 attcgtataa ttagccgata attgattgat caaagtatct aagtctacac cgtctttatt
    69481 ttctgctgcc tgatacgctg cacgtaattt tctgatccct tcgtcggaga ttgcttgaag
    69541 ctcacgtgct ttttggttag caactgctga taaaccagct attccctctt gatttccctg
    69601 aatttctgtc tggtattctt caatcaatgc aggcgtcttt tcttcgatct ttttcgaata
    69661 atcatttacg attgcttgaa gttgctctgg caaaccattt gctgtttttt cttcagaagt
    69721 ttgtgtctct tttgttttag ttgatgactc acttgtttgt ttcgtctctg ttttctttgt
    69781 cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa ttttttccat gaaaatatag
    69841 acccgcaact cctcctgcta tgatcaaaaa cacggcaact gctgccccaa tcatgatcat
    69901 ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc atccgaacat tttctcccca
    69961 ctcattttca tcatacattt ttttcaccac caatttttat gtataatagc gttatcaaac
    70021 gcttcatatt cttattgtag caaataattg tcgctgtgca catttttatc tttgttattt
    70081 ataagaaaat ttcaccagtt gagatgattc caagattgtc cgtcgatttc tccttgcagc
    70141 tgcattgttc gttcgtctat ctcagccatc agtttcttta tttcatataa gagcgcacgg
    70201 tcttgatatt ctgatttttc gcttaattca tctaattgtt ggatcagcca atcatttgtc
    70261 ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg
    70321 cctcagtcag tgcttctacg atatatgctt gtttttcaga ttcttcttcc tgcattttct
    70381 gattttcctg tactctttga ataaaccagt caatccgctg agtcagcgat ggtttgcctt
    70441 ccaatgagag atactgctga taagcaatta gcttctccca acgtttttcc tgggataaat
    70501 acataaattg tgctaaaacg aatggcggaa gataggtcat atggaattta tttgcgattc
    70561 gctgatacgg cctcaaaaat tcggaaatcg tatattcttc ttttcctcca gcttggtatt
    70621 ctttttccgc cacacctgtt gtcaatacga ttcctagttc tttccctttt aatacatgtg
    70681 ctgctcgttc caagacatta tcttgccaaa tttttaaatg agcaggagaa ctataccagt
    70741 aaaacggaaa ctggaaaatg atccgatcat gctgggccaa gagctgtagt tcagtctcta
    70801 tatctatttt tccgtctgga taacttgatt ccaaatgatg ccaagtagct ggtaaaccag
    70861 cagaagcttc tttgaaaaat tgctgattta aagattcttc gatcgttgga tggctaatga
    70921 tgataagtgt acgcatccct tttcctcctt tttagtcttc gctttacagt gtagcataac
    70981 cgccacaagt ttcttttgcc gatgcttgtc attttaacca gactaatcag gttgtccaag
    71041 aaactgcggt ttgtttttcc aactgttttt tcaaggtatc caaagcaata ttcttaaaat
    71101 tttcgttttt catacgttta cgatagttcg tagccgtttc tccggtactc tttttgaaca
    71161 ttcggctaaa tacgactaga ctattgaacc ctacttgata ggcaatactt tccacggatt
    71221 catccgtcag agaaagaaga aacgcccctc gattgatccg taccccgatc aaatattcct
    71281 tgatactggt acccatatga tttttgaata ctcgcgacaa atggctccga ttgaccgcta
    71341 aatgctgggc gatttcctca acagacaatt tttcagcata atgtgcctcg atgtatttga
    71401 ctgtttcaac agctaaagag gaatgaacct tttgtacacc accgctgacc tttccgccat
    71461 cttttaaaag gactgataaa aaacggtagg ttaatgctgt taactgtaat tcattttcca
    71521 aggtttcttc cgtatctttc atgcattcta aaataatgtc gatatatttt tgtatgtcag
    71581 aagagaccat cgtgtattgg tcttctctga aaagcgactg ccgaatgatt tcttctgcag
    71641 cactcccacc aaatgaaagc cagcaataca tccacggatc ttctccatct gataagtaaa
    71701 acgtcgagtc tcctggacga atcaagaaca gatcaccttt tttcaattgg aattgttgat
    71761 ttttcacatg ataagatcct ttgccttcca tcacgacatg caggatgtaa tcattgcgaa
    71821 tagcaggacc aaaagaatga aacggcatcg ttttagagaa accacaaaaa tcaaaataca
    71881 attctttttc tacttgttga tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc
    71941 ctccttactc ctttagtata atgcaacaaa aagttaaata aaagtcacag aaatatatta
    72001 aaaaggaagc gatttcttgt taagtttaag tcattaaaag atctttgtta ttcgttgtaa
    72061 ccggtttact tagtattata tattgaacag gagaggaata cagattatga aaagcaaaat
    72121 gatccaacgt gtgtcctacg ctttaggcgc ttttggacac gacacattct atgttacatt
    72181 aagcacttat tttatggtat tcgtgacaag ccagatgttc gcaggaacag ataaggcaac
    72241 gaatgcaaaa atgattgcta cagttacaag cttagttgta ggtatccgct tagttgaaat
    72301 tatttttgat cctttgattg gtggcatcat cgataatact cgtacaaaat acggtaaatt
    72361 caaaccatgg ttagttatcg gcggcgttgt tagttccgta ctcttggtag caatctttac
    72421 caatttcttt ggcttagcta ctcataatac gactttattt atcatattat ttgcaatcgt
    72481 tttcatctta ttggatagtt tttattcatt taaggatatc gctttttggt caatgattcc
    72541 agctttgagt acggaaacaa aggaacgtga aaaatttgct acgtttgctc gtttcggctc
    72601 ttctctaggt gcaaatggta ctactctagt cgttgtccct atcgttactt tctttaccta
    72661 tcttgttact cataaacata ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc
    72721 tgctgttatt tcaggcggta ctgcgattat cactgccttg ggcacaaaag aagtcgatac
    72781 tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat gtgttcaaag cagttgctca
    72841 aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct tatgcgattg ccaatgcagc
    72901 aacaactgcc gtattattct acttcttcaa atatgtactt ggccaaccaa ctgaattttg
    72961 gatcgttggt ctagttgctg ccttacttgg tttagtagct gttccactat tcccaatgct
    73021 tacacgcgtc atctctcgtc gctacgttta ccttggaggg atcgcaatga tgatttcttc
    73081 ttactttttc tttacgatcg caggttcaag tttgccaatc gttatcatcg ggctagtatt
    73141 gttctacttc cctcaacaat tgattttctt atccgctttg atgacgatca cagactctgt
    73201 tgaatatggt caatggaaaa atggtatccg taacgaagct gttactctat caattcgccc
    73261 tttactagat aaaatcgctg gtgctttgtc taacgggatc gtagggtttg tcgctatcgc
    73321 ttctgggatg actggtaatg ctactgctgc tgatatcact gctcatggcg taacgacttt
    73381 caaagcttat gcattctatg ctcctgctgc tttgatgatt ttctccgcta ttatttttgc
    73441 ttggaagatt acattgacag agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa
    73501 attggctcca gctgatacaa tggaagatta acttcacgat gcagtagttc cttcccatta
    73561 agaaaggatc ttgaaaatga agattacaga aaaagatttt ggccaaggtt atcacttgat
    73621 aaccttggag aacaaaaaca agctagcact ttctatctct gatttaggtg ctagaatcgt
    73681 cagtttaaaa tcaaatgatc gagaattagt tttgggattt gacaccgctg aagaatatat
    73741 cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg
    73801 ccgcttttct ctcaatggaa aaacttacca attagccaca gatccaaaaa ctggacataa
    73861 tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg
    73921 cgaaaatgaa gcttctgtca tttttactac tacaagtccc gacggagaac atggttttcc
    73981 aggtacaatg gatgtggaaa tccgctatac tttgacaaaa gataatatct ggcgagtgac
    74041 ctctcgcggc accagtgacc aagatacttt attcaatccg acaaatcatg tttacttcaa
    74101 cttgacggga gatgcttctc agtcaatcga tcaacatgag ctttggctca atagcgaagc
    74161 ttatgctcct ttgcgaacag atagtattcc tataggggta aaagaaaatg ctgcaggaag
    74221 tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt tttgctagtg atttggatca
    74281 aaaaaatctt gttgacggaa tcgatcatcc atttttctta aaagaaaccg gcttgggtaa
    74341 agaagccgct cgcttgacta gccccgataa aagaatccaa gtagatattg ctacagatgc
    74401 ttctagtgtg gttattttca ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg
    74461 aaaactggca catcacggtg gcatcacatt tgagactcaa acagctccag gtgcagagcg
    74521 cttctcagca tttggttcga ttcatttaaa agccggatca gtatttgaaa cagtaacaga
    74581 attcaaaatc aaaacaagaa aggaataact catcatggaa atcaaaagaa gcttaaacac
    74641 aaaatttgcc gaaacattcg gacctaaaaa aactacacaa tacttctcac caggacgcat
    74701 caatttgatt ggtgaacaca cagattacaa tggaggccat gtcttccccg cttccatcac
    74761 ttatggaacg caaggtgtag cagcacctcg agacgataat aaaatattag tttattccac
    74821 aaactttgac gatatcggtg tcattagttt tactttagat gagctagaat atgataaaaa
    74881 agcaaactgg gcaaattacg taaaaggaat ggtccttaaa ttaaaagaag ctggacactc
    74941 aatcgatcat gggtttgagt tattagtgga aggaacaatc cctaatggtg caggactttc
    75001 cagtagcgct tcattagaat tattagtagg tgttgtatta gaagatctgt ttgatttaaa
    75061 aacagatcgc ttggaactag tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt
    75121 caattcaggt atcatggatc agtttgccat cggcttcggt gagctagatc atgcaatctt
    75181 attagataca aatacgctga aatacgaaat ggttccagtg aaactagatg gttatgcaat
    75241 cgtcatcatg aatacgaata agcggcggga attggcagat tcaaaataca atgaacgccg
    75301 tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact ttgcctatct cttctttagg
    75361 agacttagat gaagaagaat ttgaatcaac tattgaccaa atcggcgatg atacattgat
    75421 acgcagagct cgccatgccg tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt
    75481 agaagctggg aacttagaag catttggtca actgttaaat gattctcacc actctcttcg
    75541 ctatgattat gaagtaactg ggatcgaatt agatacacta gtagatgctg ctcaaaaaca
    75601 agaaggtgtc ttaggtgcac gaatgacagg agctggattt ggtggctgtg cgattgctct
    75661 tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt
    75721 tgtcggctat gctccggaat tttatgttgc acacattgga aatgggacaa caaagatcga
    75781 tccagaagct gttcatgttt gataaaaaaa tcaacgatct cagctaaaaa caaatagata
    75841 aaaataacct ctcagcaatt tccaaatgtg agccttttgc tcgtatttga aaattgctgt
    75901 tttttattga cacggaaaga gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg
    75961 tgggcgaatc aaaatccggt atttgtttat gaaaaagaac cttcaagaga tattttatgt
    76021 attgactgca aatcttttta cgcctccgta gagtgcgttg aaaaggatct tgatcccctt
    76081 acaactaaat tagtagtcat gtcctatccg tccgattcga cagaaacacg gggtagcgga
    76141 ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt
    76201 gccagagatc ttcccttccc ctatccaagt gatttatata ttgttgcccc aaggatggcc
    76261 tattatatgg aaaagaacag agaaatcaat tctatctata aaaaatatgc agacgaagcg
    76321 aaccatcatg tctattccgt cgatgaaagc ttccttgatg taacaaatag catggcactc
    76381 tttggtaaaa caaatgcgta cgacatggct aaactgatcc ataaagatgt gtttgagcag
    76441 accggaatct acacgactat aggaattggc gataacccgc tacttgctaa actagcctta
    76501 gacaatggct caaaagacgg aaagcaaatg tttgctgaat ggcgttatga agatgtacca
    76561 gaaaagttat ggaaaatctc agatatcact gacttttggg gaatcggcaa acgaacagca
    76621 aaacgtctga aacaaatggg tatacattct gtttatgatt tagctcacgc ggattattat
    76681 cacttgaaaa gcaagctagg ggtcatcggt actcagcttt atgcccatgc ttggggcatc
    76741 gatcgaagtt ttttgggaca agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa
    76801 atcctagata gagattatac aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc
    76861 gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa cacaagtagt cagcttatgg
    76921 gtaggatact ctttaggcta tcttgatcca aatggcaaga ccgggtttca ccagcaaatg
    76981 aaaatcgaag cgacaaatgc tagcagagat cttgcagatg ctttattgac cattttccac
    77041 cagcattacc actaccagga tattcggcgt gtaggcgtga actgttccga cctcgtatac
    77101 gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa
    77161 gttgattttg ttgtcgatac gattagaaaa aaatacggct ttcaatcaat catccatgcg
    77221 catagtctct tgcaaggagg acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca
    77281 ggtggtatga gtggattaga aggtggaaaa gatgagacgg acagcaaaaa aattcattga
    77341 ctataacgag tatcgagatc ggccatttgg attaaaatgg gggactgctt ttgcgatgga
    77401 agaactagtc aacggtatca aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa
    77461 caacaaatga cattgcaaga aatcgatagt atactgacag aagccttttt atctcgtaaa
    77521 gcagtaatca ttcagctcaa tttaaaagat gaatttggaa gactcttgga tgaggtcgaa
    77581 gggcacttca ttggtgaagc ctatgaagat tactttatga ttgaccatca gccgatattg
    77641 tgggaagacg tacggtatat tgagttaaag gaaacaaaaa aatggtttgt gacatgagag
    77701 gttgtgacag aagtgatcaa cttcaaaaaa taagaaagga tttttcgaaa attgcttctt
    77761 aaatttttgt ggaattttgg cttatttttc gaagaagcaa cttctggaac aacgtatatt
    77821 agatatcaga ggttgtgaaa aagtcgctta gttttgagta acaagccaag gcgctgattt
    77881 cacaacctct gtctatctat ccatctattc ggtaagattc atgcatgacg tcttctttat
    77941 ttagtatgta gagccctttt tcttctgacc attcaacata aatcagctgt tttacatctg
    78001 catagccttc tttcttcaag ttctccataa gccatgcttc agtcaattga ttggactgaa
    78061 gcactttatg ctgaatttgc ccttcatcta caagcaggat cgataaagct ttgtcttcat
    78121 ctgatttttt caatacactc atttgaccgg cattctctag gataacatgt ttggcatttt
    78181 caagaaaaaa gcattcttgc tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt
    78241 tatttgtcaa ctctgtcatg ttgatgacgc cgtctttgat tagaacagat ggctcgcctt
    78301 tgacccaacg attgaccttt tcattttttt ggacgatacg ttcgatacca taaatcatta
    78361 ctgcccaaag cgcaatggca aataggacat gccctacatg gacattgtca tcataggttg
    78421 attcttctaa gatcccacct aaaacaagcg tatagactaa atcaaacggt gtgatttctg
    78481 acatggattt cttcccaagc aaacgtacca ctaataataa accgatcaac ccgaacacca
    78541 gtttcattgt aattgaaaaa atcatttttt ctccccctaa ctatctgtta attaaattat
    78601 atatgctttc tcacgaacac gcatggaata tacaaggaag aaatgtatcc ttttactaaa
    78661 aaaaggactg tgacactgta gtgaccccca aaagttagac ttttttggag tggagaaatc
    78721 cactcctttt ttttatgctc taaaatgata tttttacaca accgttacgg ttgttttacg
    78781 aaattggacg
//
LOCUS       DANKHJ010000005        65936 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000005 DANKHJ010000000
VERSION     DANKHJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            621..1484
                     /locus_tag="PW113_000409"
     CDS             621..1484
                     /locus_tag="PW113_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2524747.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1613..3235)
                     /locus_tag="PW113_000410"
     CDS             complement(1613..3235)
                     /locus_tag="PW113_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2524748.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3484..4005
                     /locus_tag="PW113_000411"
     CDS             3484..4005
                     /locus_tag="PW113_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2524749.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4191..5921)
                     /gene="spxB"
                     /locus_tag="PW113_000412"
     CDS             complement(4191..5921)
                     /gene="spxB"
                     /locus_tag="PW113_000412"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2524750.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6086..6904
                     /locus_tag="PW113_000413"
     CDS             6086..6904
                     /locus_tag="PW113_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2524751.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7007..7204)
                     /locus_tag="PW113_000414"
     CDS             complement(7007..7204)
                     /locus_tag="PW113_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524752.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7373..7951
                     /locus_tag="PW113_000415"
     CDS             7373..7951
                     /locus_tag="PW113_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2524753.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            8046..8183
                     /locus_tag="PW113_000416"
     CDS             8046..8183
                     /locus_tag="PW113_000416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524754.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8996..9499)
                     /locus_tag="PW113_000417"
     CDS             complement(8996..9499)
                     /locus_tag="PW113_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2524755.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9858..10883
                     /locus_tag="PW113_000418"
     CDS             9858..10883
                     /locus_tag="PW113_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2524756.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11206..12084
                     /locus_tag="PW113_000419"
                     /pseudo
     CDS             11206..12084
                     /locus_tag="PW113_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            12560..12676
                     /locus_tag="PW113_000420"
     CDS             12560..12676
                     /locus_tag="PW113_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2524757.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13604..13942
                     /locus_tag="PW113_000421"
     CDS             13604..13942
                     /locus_tag="PW113_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524758.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14036..14266
                     /locus_tag="PW113_000422"
     CDS             14036..14266
                     /locus_tag="PW113_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524759.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14666..15037
                     /locus_tag="PW113_000423"
     CDS             14666..15037
                     /locus_tag="PW113_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2524760.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15311..16003)
                     /locus_tag="PW113_000424"
     CDS             complement(15311..16003)
                     /locus_tag="PW113_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2524761.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(16006..16791)
                     /locus_tag="PW113_000425"
     CDS             complement(16006..16791)
                     /locus_tag="PW113_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2524762.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            17028..18428
                     /gene="lacG"
                     /locus_tag="PW113_000426"
     CDS             17028..18428
                     /gene="lacG"
                     /locus_tag="PW113_000426"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2524763.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18453..18770
                     /locus_tag="PW113_000427"
     CDS             18453..18770
                     /locus_tag="PW113_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2524764.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18783..20456
                     /locus_tag="PW113_000428"
     CDS             18783..20456
                     /locus_tag="PW113_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2524765.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20470..22101
                     /locus_tag="PW113_000429"
     CDS             20470..22101
                     /locus_tag="PW113_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2524766.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22592..24157)
                     /gene="guaA"
                     /locus_tag="PW113_000430"
     CDS             complement(22592..24157)
                     /gene="guaA"
                     /locus_tag="PW113_000430"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2524767.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24216..25139)
                     /locus_tag="PW113_000431"
     CDS             complement(24216..25139)
                     /locus_tag="PW113_000431"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2524768.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25335..25940
                     /locus_tag="PW113_000432"
     CDS             25335..25940
                     /locus_tag="PW113_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2524769.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26248..27264
                     /locus_tag="PW113_000433"
     CDS             26248..27264
                     /locus_tag="PW113_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2524770.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27405..28706
                     /locus_tag="PW113_000434"
     CDS             27405..28706
                     /locus_tag="PW113_000434"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2524771.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28752..29414
                     /gene="deoC"
                     /locus_tag="PW113_000435"
     CDS             28752..29414
                     /gene="deoC"
                     /locus_tag="PW113_000435"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2524772.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29435..29833
                     /locus_tag="PW113_000436"
     CDS             29435..29833
                     /locus_tag="PW113_000436"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2524773.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29899..30981
                     /locus_tag="PW113_000437"
     CDS             29899..30981
                     /locus_tag="PW113_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2524774.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31193..32755
                     /locus_tag="PW113_000438"
     CDS             31193..32755
                     /locus_tag="PW113_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524775.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32748..33881
                     /locus_tag="PW113_000439"
     CDS             32748..33881
                     /locus_tag="PW113_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524776.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33881..34837
                     /locus_tag="PW113_000440"
     CDS             33881..34837
                     /locus_tag="PW113_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524777.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34936..36102
                     /gene="deoB"
                     /locus_tag="PW113_000441"
     CDS             34936..36102
                     /gene="deoB"
                     /locus_tag="PW113_000441"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2524778.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            36126..36944
                     /locus_tag="PW113_000442"
     CDS             36126..36944
                     /locus_tag="PW113_000442"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2524779.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36961..37668
                     /gene="deoD"
                     /locus_tag="PW113_000443"
     CDS             36961..37668
                     /gene="deoD"
                     /locus_tag="PW113_000443"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2524780.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37966..38652)
                     /gene="gpmA"
                     /locus_tag="PW113_000444"
     CDS             complement(37966..38652)
                     /gene="gpmA"
                     /locus_tag="PW113_000444"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2524781.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38824..39405)
                     /locus_tag="PW113_000445"
     CDS             complement(38824..39405)
                     /locus_tag="PW113_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2524782.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39538..40221)
                     /gene="rpiA"
                     /locus_tag="PW113_000446"
     CDS             complement(39538..40221)
                     /gene="rpiA"
                     /locus_tag="PW113_000446"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2524783.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40540..40953
                     /gene="rpsL"
                     /locus_tag="PW113_000447"
     CDS             40540..40953
                     /gene="rpsL"
                     /locus_tag="PW113_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2524784.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            41037..41507
                     /gene="rpsG"
                     /locus_tag="PW113_000448"
     CDS             41037..41507
                     /gene="rpsG"
                     /locus_tag="PW113_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2524785.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41601..43688
                     /gene="fusA"
                     /locus_tag="PW113_000449"
     CDS             41601..43688
                     /gene="fusA"
                     /locus_tag="PW113_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2524786.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43860..45047
                     /gene="tuf"
                     /locus_tag="PW113_000450"
     CDS             43860..45047
                     /gene="tuf"
                     /locus_tag="PW113_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2524787.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45454..45762
                     /gene="rpsJ"
                     /locus_tag="PW113_000451"
     CDS             45454..45762
                     /gene="rpsJ"
                     /locus_tag="PW113_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2524788.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45793..46422
                     /gene="rplC"
                     /locus_tag="PW113_000452"
     CDS             45793..46422
                     /gene="rplC"
                     /locus_tag="PW113_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2524789.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46450..47073
                     /gene="rplD"
                     /locus_tag="PW113_000453"
     CDS             46450..47073
                     /gene="rplD"
                     /locus_tag="PW113_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2524790.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            47073..47363
                     /gene="rplW"
                     /locus_tag="PW113_000454"
     CDS             47073..47363
                     /gene="rplW"
                     /locus_tag="PW113_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2524791.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47402..48235
                     /gene="rplB"
                     /locus_tag="PW113_000455"
     CDS             47402..48235
                     /gene="rplB"
                     /locus_tag="PW113_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2524792.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48278..48556
                     /gene="rpsS"
                     /locus_tag="PW113_000456"
     CDS             48278..48556
                     /gene="rpsS"
                     /locus_tag="PW113_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2524793.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48579..48926
                     /gene="rplV"
                     /locus_tag="PW113_000457"
     CDS             48579..48926
                     /gene="rplV"
                     /locus_tag="PW113_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2524794.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48940..49596
                     /gene="rpsC"
                     /locus_tag="PW113_000458"
     CDS             48940..49596
                     /gene="rpsC"
                     /locus_tag="PW113_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2524795.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49599..50033
                     /gene="rplP"
                     /locus_tag="PW113_000459"
     CDS             49599..50033
                     /gene="rplP"
                     /locus_tag="PW113_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2524796.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            50023..50211
                     /gene="rpmC"
                     /locus_tag="PW113_000460"
     CDS             50023..50211
                     /gene="rpmC"
                     /locus_tag="PW113_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2524797.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50236..50502
                     /gene="rpsQ"
                     /locus_tag="PW113_000461"
     CDS             50236..50502
                     /gene="rpsQ"
                     /locus_tag="PW113_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2524798.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50560..50928
                     /gene="rplN"
                     /locus_tag="PW113_000462"
     CDS             50560..50928
                     /gene="rplN"
                     /locus_tag="PW113_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2524799.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50965..51273
                     /gene="rplX"
                     /locus_tag="PW113_000463"
     CDS             50965..51273
                     /gene="rplX"
                     /locus_tag="PW113_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2524800.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51301..51840
                     /gene="rplE"
                     /locus_tag="PW113_000464"
     CDS             51301..51840
                     /gene="rplE"
                     /locus_tag="PW113_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2524801.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51859..52044
                     /locus_tag="PW113_000465"
     CDS             51859..52044
                     /locus_tag="PW113_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2524802.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            52080..52478
                     /gene="rpsH"
                     /locus_tag="PW113_000466"
     CDS             52080..52478
                     /gene="rpsH"
                     /locus_tag="PW113_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2524803.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52510..53046
                     /gene="rplF"
                     /locus_tag="PW113_000467"
     CDS             52510..53046
                     /gene="rplF"
                     /locus_tag="PW113_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2524804.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53207..53563
                     /gene="rplR"
                     /locus_tag="PW113_000468"
     CDS             53207..53563
                     /gene="rplR"
                     /locus_tag="PW113_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2524805.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53584..54084
                     /gene="rpsE"
                     /locus_tag="PW113_000469"
     CDS             53584..54084
                     /gene="rpsE"
                     /locus_tag="PW113_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2524806.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            54099..54278
                     /gene="rpmD"
                     /locus_tag="PW113_000470"
     CDS             54099..54278
                     /gene="rpmD"
                     /locus_tag="PW113_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2524807.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54322..54762
                     /gene="rplO"
                     /locus_tag="PW113_000471"
     CDS             54322..54762
                     /gene="rplO"
                     /locus_tag="PW113_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2524808.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54762..56057
                     /gene="secY"
                     /locus_tag="PW113_000472"
     CDS             54762..56057
                     /gene="secY"
                     /locus_tag="PW113_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2524809.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            56119..56766
                     /locus_tag="PW113_000473"
     CDS             56119..56766
                     /locus_tag="PW113_000473"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2524810.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56957..57175
                     /gene="infA"
                     /locus_tag="PW113_000474"
     CDS             56957..57175
                     /gene="infA"
                     /locus_tag="PW113_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2524811.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57208..57324
                     /gene="rpmJ"
                     /locus_tag="PW113_000475"
     CDS             57208..57324
                     /gene="rpmJ"
                     /locus_tag="PW113_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2524812.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57342..57707
                     /gene="rpsM"
                     /locus_tag="PW113_000476"
     CDS             57342..57707
                     /gene="rpsM"
                     /locus_tag="PW113_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2524813.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57735..58124
                     /gene="rpsK"
                     /locus_tag="PW113_000477"
     CDS             57735..58124
                     /gene="rpsK"
                     /locus_tag="PW113_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2524814.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58204..59142
                     /locus_tag="PW113_000478"
     CDS             58204..59142
                     /locus_tag="PW113_000478"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2524815.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59170..59550
                     /gene="rplQ"
                     /locus_tag="PW113_000479"
     CDS             59170..59550
                     /gene="rplQ"
                     /locus_tag="PW113_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2524816.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59822..60661
                     /locus_tag="PW113_000480"
     CDS             59822..60661
                     /locus_tag="PW113_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2524817.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60637..61506
                     /locus_tag="PW113_000481"
     CDS             60637..61506
                     /locus_tag="PW113_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2524818.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61496..62293
                     /locus_tag="PW113_000482"
     CDS             61496..62293
                     /locus_tag="PW113_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2524819.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62496..63242
                     /gene="truA"
                     /locus_tag="PW113_000483"
     CDS             62496..63242
                     /gene="truA"
                     /locus_tag="PW113_000483"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2524820.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63907..64144
                     /locus_tag="PW113_000484"
                     /pseudo
     CDS             63907..64144
                     /locus_tag="PW113_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(64288..65064)
                     /locus_tag="PW113_000485"
     CDS             complement(64288..65064)
                     /locus_tag="PW113_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2524821.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            65187..65594
                     /locus_tag="PW113_000486"
     CDS             65187..65594
                     /locus_tag="PW113_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDL2524822.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        20959 a        11760 c        13884 g        19333 t
ORIGIN      
        1 ccgtgtcaca gtcccttttt ccgaacaaac ggtgttcaaa agcttgcatc tacggcacta
       61 agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt actgcttgat
      121 gcagagcagc tttttcacaa cctctccact aaacggtgtt ctcctgccaa ctcctcggtc
      181 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
      241 ggagctaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
      301 tattagctca aaaaacggca aaatatgagg aactattttt tccgtttttt tctctaatac
      361 tcaggtcaaa acgctgattt cacaacctct gattcacggt gttccagaag tagcttctac
      421 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
      481 tcttgaagct gaccacttct gtcacaacct cttattttga ttagaacttg cgaaaataaa
      541 ccggttaaag tgtgtctagc gcaagagatt tgctataata gacgataagg gtaaaatgta
      601 acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga ttcttgttgt gatctgccat
      661 accatgtatt agcaaaagaa aaagttgatt ttttatcaat gaatctccaa ttgaatgatc
      721 aagagttagt agatgacctc ggaaagacat tcaattatga ccgctacatg aatcagctga
      781 aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg ccgatattta gaatttttcc
      841 gttcttatgc ggaaaaacag attcctgttt tatatgtggc tttttccagt ggattaagcg
      901 gctcttacag tagtgcgctt caagcggttg aattgctgaa ggaagaatat gaagaccctg
      961 agatctatgt ttttgataca aaagctgcaa gtattgggga agggctttta gtattggaag
     1021 ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac cctagagtgg ctggaaagta
     1081 attatttgaa agtccattca tgggtaacag tagacgatct gaaatatcta gaacgaggcg
     1141 gtaggatttc caaagcaagt gctactcttg gcagtctatt gagtgtaaag cctattatta
     1201 cggtggataa agaaggaagg ctgacgaatg ttgacaaagt tcgaggaaga aacaagtcga
     1261 tccagacaat cgtcaatgag acggtaaaaa caatcgttgc accacttgat caaatcgttt
     1321 atgtggctta cgctggggat ctagagagcg cggagaaggc aaaacggctt ttggaagagc
     1381 agataaagat gaaagatgtg aagctttatc ccttaggacc aacgatcgcc agtcacacag
     1441 gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg ataaaagcag taactcattc
     1501 tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga ctcttaaaag atagatgagc
     1561 atcctatctc ttagaggtcg ctgttttttt attttttaag ctaaataggc cattatgccc
     1621 ataactcgtt caattgtttt tgaatatctg cattttccaa aaattcatca tatgtcgtat
     1681 cagctcgatc gatcacacct ttatccgaaa cggcaatgat gcggtttgcc aatgtttgga
     1741 taaattgatg gtcatgtgac gcaaataaga ttgagccagt aaatgccatc aagccgtcat
     1801 tcaatgctgt gattgattct aagtctaaat ggttcgttgg atcatctaat acaagtacgt
     1861 tagctttcga aagcatcatt tttgaaagca tgcaacggac tttctctcca ccagataaaa
     1921 cattcactgg tttcaatact tcttcaccag aaaacaacat tcttcctaag aagcttctta
     1981 agaaggtgtt atcatcttct tctttacttg catattgacg caaccagtct aaaatcgtta
     2041 gatcagtatc gaattctttc gttgtatcct ttggtaaata agaattactt gttgtcacac
     2101 cccaacgtac tgttccagta tctggcgtaa tttcacccat gataactttg aatagcgttg
     2161 ttgttgttat atcattttct gcgacgaaag cgaccttgtc ttctttgctc aaattaaaag
     2221 atatatcatc taaaattttc tttccatcaa tcgttacact tacattttct acctgcaata
     2281 agtcgtttcc aatctcacgt tcaggtttga atccaacgaa tggataacgt ctagaagaag
     2341 gttggatatc gtctaaagta attttttcaa gcattttttt acgtgaagtt gcttgttttg
     2401 attttgatgc attcgcacta aagcgggcaa taaattcttg gagttctttg atttgttctt
     2461 cttttttggc atttgcgttt gcttgaagtt tagccgctag ttgactagat tccaaccaaa
     2521 aatcataatt tcctacataa agtttgattt taccaaaatc tagatccgcc atatgtgtac
     2581 atactttgtt caaaaagtga cggtcatggg atacaacgat cacagtattt tcaaaattga
     2641 tcaaaaattc ttccagccaa ttgatcgatt ggatatccag accgtttgtt ggttcgtcta
     2701 gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa taacactttt accttttgtc
     2761 caccagttaa ttcactcatt tgctgatggt gcaattcttc agggatgttc agaccttgca
     2821 gtaaaacagc tgcttctggt tcagcttccc atccattaag ctcagcaaat tctccttcta
     2881 attcagctgc tcgaatacca tcttcgtcag tgaaatcctc tttcatgtaa atcgcatctt
     2941 tttctttcag cacttcatac agacgcttgt gtcccatgat gactgtttca agtacagtat
     3001 agtcttcata gtcgtaatgg ttctgtttca atacggccat ccgttcatct ggtcccatag
     3061 taacggaacc tgtggaaggc tgcagatccc ctgataacac tttaaggaac gttgatttac
     3121 ctgcaccatt tgcaccaatc aagccatagc agtttccggg ggtaaattta atattcacat
     3181 catcaaatag tttgcggtct gaaaagtgca aactcacatc atttacagta atcaattttt
     3241 ttcctcactt ttatcattat tagttttgac aagtcttata gaattatata ggggaaaggt
     3301 aaaagattca aggcaatcga acattctttg tgtttcacgt gaaacagaat gaaaaaaata
     3361 aaaataaaag cggagcgaaa atagctacta taggatttat tagtttgcaa cagcataatt
     3421 tttgacgtta tttataagag ggagtactat aaaactgtaa aagaaatgaa agaaggattg
     3481 aaaatggatg aaaaaagaac catcgactgg ggctcattga ttttgggaat tttatttgta
     3541 cttgtctcat tgttatcatt tcaagaccct gtcggaaatt tagttgcaat tgtagtagtc
     3601 tttgcgattt ttgcctttat taaagggata tttgaactgt ttgtacggaa cagattaaaa
     3661 gagctaacag gatataaagg aaagatgccc ttaataatcg ggatcatcga tattctagtt
     3721 ggcgtctttt tcttattcaa tattggtgca ggagtcgcag cattgccttt tgtttttgca
     3781 gtatggttta ttgctgattc agttttagcg ttatttactg ctgacttggc aagaggagta
     3841 agtgaaggat attattggtt tacgatcatc gtcaatattt taggaatttt attaggaatt
     3901 ttcttattgt ttaatccaat ttcttctgcg ttgacattaa gctttttagt tggattctat
     3961 tttatgttat ttggtatcac acatatcgtc tatgcattta gataagtaaa atcaaacaaa
     4021 taaaaaagcc ggcttccgat tacagtttta ctgttgtaat tggaagccgg cttttttatc
     4081 atggacaaat atgcacgagg tgatttttag gtgtcgagac aaatttatgc aaaaatgtga
     4141 gaagctatat acacaaattt tatcttaata ctcgaagcat agcgcagttg tcacaacctc
     4201 ttttcatata attcattcaa tactggcaaa tcatggactt catatttttc tttgaatgct
     4261 tggatttctt cttcagaata ttttcttgga tctaaatgca gatcttctac tggcaatggg
     4321 cgcttgttat tgatcttcac atcgattact acaggacgat ctgattttgc agctgcatca
     4381 aaggcaaatc gaagttgcga gtattctgtg actgtaaagc tatttgcccc gagcgcttct
     4441 ccaactttac cgaaatctgc atcttctaaa aatacaccaa atttcttttg ttccgtatct
     4501 tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa agacaacatt aataattgga
     4561 agctgatatt tgacttgtgt aatgatatct tgcatgacca tagcaaaccc gccatcacca
     4621 cttaaggtaa atacttgttt ttctgggtag cttagttgtg cggcaattcc tcctggcaca
     4681 ccattaccca tagttgcgaa ccatccagat gtcgtatgtt tttgtttacc gttcatattc
     4741 aataaacgaa tagaatggat agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct
     4801 tcagcaatac gattgatttc tttatagaca gcctctggac gaatcggttc ttcctctcga
     4861 tcttcaaaag atttcaacca atttacccat ttttctttat tttcttgatt tgctaggtac
     4921 cagctatcag cagggcgagc atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca
     4981 tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg
     5041 atttggataa attcagcagt tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg
     5101 acaaataaga tcagatcggc ttctgccaat gcttcatttc cgggtttagt agcgacacgt
     5161 ccagcaaaac caagaaagtt ttcataatca tcaggaataa ttccttttgc tagaacggaa
     5221 gcgacaacag gaactgaaaa atgttctgag aattctttga tttgcggaaa tccgtttcgc
     5281 gttccttgac caacataaag aactggtttt tttgcttttt caagataagg caaagcagct
     5341 agcaaatctt tttcttctgg taatatcaca cttgttttgt gagtgtgagc ggtggagaaa
     5401 ggttgttcgt caatttcttc aaaaccaaga tcaacaggga tagtcacgat agccacgcct
     5461 ttatgctcat aggcggcttt aatggcttca tctactacat gcggaagact ttgtggtgtc
     5521 atgactgtac gattgtatac gctaacatct gcgaaaatag gattttcatt tagttcctgg
     5581 aaagagttat agttcatgga tgtagaagca acttgtccta agaacgctac tagaggcaca
     5641 tggtccatct gtgcatcata caaaccattg atcaaatgtg tcgcaccagg tcctgcagag
     5701 ccgaagacag cgccgacttt ccccgttaat tttgcatctg cagcggctgc gagtgcgccg
     5761 acttcttcat gacgaacttg aatgtagttg atttcggttt tttcttgata tagcgcatcc
     5821 ataattgaat tgaaagatcc tccgggaatg ccgtaaatat ggtcgatacc ccagctttca
     5881 agaactttta ccattgcgat tcctgcattg atttttgaca tggtgtttca ctcctttagc
     5941 aatgatgata cataaatttt ttgtatctag agtatagaac tttttatcga aaatcccaac
     6001 taattcgtct tttcacaaaa atgtagtcat gtttttggga atatgctaga gtagtacata
     6061 gatagattca agagagggga atggcatggt tgaatggcta ttgagacttg aaccttggca
     6121 gcaagcactt gctgggacag cttttactta ttttatgaca gctcttggtg ccgggttggt
     6181 ttttttcttt aaagaaatca aaaaagaagt gctaaacttg atgttaggat ttgcttctgg
     6241 tgtcatgatt gctgcaagtt tttggtcttt gttagatccg gctattacaa aagcagaaga
     6301 aaatggagat attgcttggc tagttgtaag tattgggttt ggtttaggcg gtgtgttttt
     6361 atatatggca gataaaacac tgccccatat gcattttgga ccacaacatg aagcagaagg
     6421 attgcctacc catttaaaac ggacgatctt gcttgttttt tctattacat tgcataacat
     6481 tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca gcaactgcgg ataatccgac
     6541 agccgctata ttagccgctg tttcggtggc gctaggtatt ggtatccaaa acttcccaga
     6601 aggagcagct gtatcaatcc cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt
     6661 ttacggacaa gcttctggaa tcgttgaacc aattgctgga gtgattggag cgctactagt
     6721 aacgaaagtg gagctgctgc ttccttatgc acttgctttt gccgcaggag caatgattta
     6781 tgtcgtggtg gaagaattga tccctgaagc acagcagaca ttgtccagca aacgtcattt
     6841 tgcagttttt ggcgtcatga gtggatttat cattatgatg attttggatg tggcattagg
     6901 ataaataaaa aaaatatata tgatatatca aatagggccg cgaacttata tcgcgaccct
     6961 atttttttag taagttatat cataatgaag tgaatggtat catacattaa taatagtttt
     7021 ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg attgggatta
     7081 gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga ctgatgacga
     7141 agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata tttttttgac
     7201 gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac agggattgtt
     7261 taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga tcaaacaatt
     7321 gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata tcatgcctaa
     7381 aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta ttttagtttt
     7441 agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag gttaccgatt
     7501 ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc atcatcattt
     7561 atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt tctttgtttt
     7621 tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca atagcctggc
     7681 tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt atatcttggc
     7741 aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga tgttgaataa
     7801 ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct tagggtattt
     7861 agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc
     7921 aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat ttaagagaaa
     7981 aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt caaagaggga
     8041 tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc acgatgctta
     8101 tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga tttttgacat
     8161 tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat ctatttaaga
     8221 aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta
     8281 atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg ataacttcgt
     8341 aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt gataggagat
     8401 atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg gtgtggtttt
     8461 tggaataaaa aaataacata tctcatttta cacttttgtt attttatact taaaaaataa
     8521 cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta ctgttttttt
     8581 cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa
     8641 gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc
     8701 tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag
     8761 attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc
     8821 tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca
     8881 ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga
     8941 ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt
     9001 gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt
     9061 atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt
     9121 gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg
     9181 ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa
     9241 tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag
     9301 gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa
     9361 aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat
     9421 taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa
     9481 attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc
     9541 tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag
     9601 aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg
     9661 aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt
     9721 ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac
     9781 taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac
     9841 taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata caagataatc
     9901 cttttcgtat catgaatctg atgctactta tctttattgt attatggtta ggttacataa
     9961 atcatgattt tttgatttca agtttttcat cattagggat ctttactttt ttctattatc
    10021 aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg
    10081 ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag
    10141 ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg
    10201 gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat
    10261 taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga
    10321 ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa
    10381 gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc
    10441 tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat
    10501 gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc
    10561 tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc
    10621 agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta
    10681 gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta tctacactta
    10741 ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa
    10801 gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg
    10861 ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag
    10921 atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa
    10981 attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa
    11041 attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa
    11101 gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa
    11161 gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat
    11221 atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact
    11281 ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat
    11341 gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca
    11401 aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat
    11461 gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag aactatctat
    11521 ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc
    11581 tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt
    11641 ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa
    11701 ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca gaataagata
    11761 agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac
    11821 caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaata gttgaaaaaa
    11881 gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac
    11941 gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa
    12001 agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct
    12061 ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga
    12121 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    12181 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    12241 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa
    12301 cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa
    12361 gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt
    12421 caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc
    12481 atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag
    12541 aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa
    12601 aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa
    12661 tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc
    12721 ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc
    12781 atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat
    12841 tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg
    12901 tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac
    12961 cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca
    13021 taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt
    13081 tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc
    13141 agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg
    13201 aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta
    13261 ctattgctag aataacatca aggaataata agttttggtt ttcagataat agtcgataag
    13321 ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc
    13381 actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa
    13441 atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt
    13501 caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat
    13561 tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc
    13621 aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa
    13681 tattgttccg gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat
    13741 aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac
    13801 agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac
    13861 tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa
    13921 aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa
    13981 aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct
    14041 ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga
    14101 aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt
    14161 ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt
    14221 agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta
    14281 tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac
    14341 taactttaat gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag
    14401 taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt
    14461 tcttaattag cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta
    14521 tagaatcaac actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga
    14581 aatttctcct ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta
    14641 ctttgcggag tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt
    14701 catcaatcaa tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc
    14761 cattatttct tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga
    14821 taaagacaaa attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga
    14881 tcttaagttt aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa
    14941 agatacggaa acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac
    15001 tcagcttggt ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa
    15061 agtatatcaa aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa
    15121 cactattttt aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa
    15181 aaagtcaaag atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt
    15241 ttcactggca gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta
    15301 gttgaatcgt ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa
    15361 aatctttgat ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac
    15421 cgaatggatc aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg
    15481 agtaagcatc ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt
    15541 taagaaaaat atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt
    15601 cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga
    15661 cagtagccgt ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt
    15721 caagcccttt tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc
    15781 tttctttttc ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat
    15841 gtttcaaaat ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata
    15901 aaccatcttt tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt
    15961 ccaaaaacat tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt
    16021 ggtattttcg gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac
    16081 ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac
    16141 ataaaaatca tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt
    16201 atttagagcg atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt
    16261 gtgaacgcca tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt
    16321 ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat
    16381 ttctggtcca tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg
    16441 aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa
    16501 aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg
    16561 aatctgcaga gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact
    16621 ttgagcccct ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct
    16681 gcgaacggtc atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc
    16741 ttttttattg accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac
    16801 ctctaattta aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa
    16861 gtaaacattt agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt
    16921 tttatgatta tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat
    16981 cagttagaaa gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac
    17041 cagaagattt tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg
    17101 aaggtggcaa aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc
    17161 ccgatccggc aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt
    17221 ttggtgtaaa tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg
    17281 ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa
    17341 gaaacgtaac gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc
    17401 atggagattt tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt
    17461 ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa
    17521 ccaatcaata tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg
    17581 tcgcctgtct tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa
    17641 acgaacttcc gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag
    17701 acgcgccgga agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat
    17761 tagatgctac ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat
    17821 gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag
    17881 catccacgcg taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt
    17941 acgatggaga aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata
    18001 aagtgaaggg aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact
    18061 ggctgattta tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac
    18121 attacaacaa aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg
    18181 gcattattat ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca
    18241 aggcgataac agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt
    18301 tctcatggac aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc
    18361 agaagcgtta cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga
    18421 ttatttaata agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg
    18481 gttttgagat cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag
    18541 aagtaagaca aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt
    18601 taacccttgc acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga
    18661 tggagatggg attcattttt atccatgggc aggatcattt aatgaccact ttattgctga
    18721 gagatctgat tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg
    18781 ctatggatgg aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac
    18841 gtaatagtta tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat
    18901 tttcaagcat tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg
    18961 aatctgtctc cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac
    19021 tcgtttcagc aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa
    19081 acaaagtaat caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa
    19141 gcgtctctcc gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg
    19201 cttcctttgt cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata
    19261 ttaccattaa aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt
    19321 ttcctttctc tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct
    19381 tccaaatctc atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg
    19441 acgggtactt aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg
    19501 ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa
    19561 ccaatctgca attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag
    19621 ggaattttgt aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc
    19681 tactctttgc taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa
    19741 gtttcgctgt caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt
    19801 ttattccatt tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg
    19861 tattgggcat gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt
    19921 tggttctagg aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg
    19981 ttgacttcct gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag
    20041 aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg
    20101 aagtacctgt ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg
    20161 tactttgtgc tggcgcaggg acaagtggga tgttagctaa tgctttaaaa aaaggtgcat
    20221 ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc
    20281 tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca
    20341 aagaagacac cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg
    20401 gattaacgag agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga
    20461 ggagccataa tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta
    20521 ggggctttgg gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga
    20581 gcagatattt cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat
    20641 atttcaaaag atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt
    20701 ttgtggcagg atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg
    20761 gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat
    20821 tttcactaca gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa
    20881 ggtctgacct ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg
    20941 gaaatgaagc agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc
    21001 ggaatgctct ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg
    21061 gctgcttttt tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca
    21121 gtaatgctcc acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa
    21181 atcacgagtc gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac
    21241 ggttcaatga atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa
    21301 gtgatcgtag ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat
    21361 gcttttggtg aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca
    21421 gagtatttgc tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc
    21481 ttcttctatt gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt
    21541 atgaattatc tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt
    21601 gattttgctg gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata
    21661 aatctagtta aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg
    21721 caaacctggt ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt
    21781 tttggggact acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa
    21841 aatgttagtg gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga
    21901 gagtttgata ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg
    21961 gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt
    22021 gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt
    22081 gtttcattgg tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc
    22141 agccacatcg ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata
    22201 accagaaacg atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca
    22261 ctttgtatta tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa
    22321 aagttgtgca gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga
    22381 atatgatttg agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga
    22441 gaacttcttt ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag
    22501 ggaaccatct tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat
    22561 agggattccc cctccatatt atgttggctc tttattccca ctccactgtt gctggtggtt
    22621 tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag
    22681 agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg
    22741 aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga
    22801 cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag
    22861 ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag
    22921 tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga
    22981 ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt
    23041 ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag
    23101 atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt
    23161 gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat
    23221 atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact
    23281 tgtctaagaa gcgatctctt gcattgactt tgatgatgtt caagccgaat tttccgccta
    23341 gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag
    23401 atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc
    23461 cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt
    23521 gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa
    23581 agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac
    23641 cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga
    23701 ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt
    23761 gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt
    23821 tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt
    23881 gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc
    23941 caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat
    24001 tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa
    24061 gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa
    24121 tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa
    24181 attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa
    24241 tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag
    24301 atatattcct gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag
    24361 gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc
    24421 gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca
    24481 tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat
    24541 tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg
    24601 tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca
    24661 gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct
    24721 ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc
    24781 gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg
    24841 gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact
    24901 tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata
    24961 taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg
    25021 cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta
    25081 cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata
    25141 ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc
    25201 accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt
    25261 cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga
    25321 gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt
    25381 ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg
    25441 tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg
    25501 aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt
    25561 tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg
    25621 taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat
    25681 cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga
    25741 tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga
    25801 agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga
    25861 aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca
    25921 tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg
    25981 gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga
    26041 actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata
    26101 aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc
    26161 aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg
    26221 gaatttagat agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg
    26281 gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt
    26341 gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat
    26401 ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc
    26461 actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc
    26521 tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa
    26581 gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag
    26641 aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga
    26701 ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct
    26761 gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga
    26821 tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg
    26881 ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca
    26941 tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg
    27001 gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca
    27061 gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg
    27121 cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa
    27181 tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc
    27241 gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag
    27301 gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct
    27361 aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc
    27421 tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca
    27481 cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt
    27541 tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg
    27601 gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg
    27661 gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag
    27721 tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag
    27781 caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa
    27841 gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg
    27901 ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga
    27961 gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg
    28021 ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga
    28081 aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag
    28141 ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag
    28201 aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag
    28261 ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt
    28321 tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc
    28381 cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg
    28441 tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta
    28501 aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg
    28561 acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt
    28621 ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt
    28681 tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa
    28741 ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat
    28801 ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct
    28861 gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg
    28921 tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa
    28981 caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt
    29041 tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa
    29101 aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca
    29161 tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt
    29221 tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag
    29281 aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg
    29341 aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg
    29401 gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat
    29461 caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg
    29521 cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc
    29581 atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga
    29641 aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg
    29701 tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg
    29761 agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga
    29821 aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt
    29881 ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta
    29941 gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc
    30001 aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac
    30061 cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt
    30121 tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca
    30181 aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta
    30241 aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat
    30301 gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct
    30361 tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc
    30421 ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca
    30481 ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct
    30541 gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc
    30601 ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa
    30661 tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa
    30721 tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga
    30781 acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac
    30841 actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa
    30901 gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca
    30961 gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag
    31021 gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg
    31081 ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg
    31141 aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca
    31201 agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa
    31261 tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg
    31321 tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa
    31381 aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg
    31441 gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat
    31501 cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga
    31561 gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga
    31621 tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga
    31681 tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat
    31741 cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt
    31801 agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg
    31861 tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac
    31921 ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga
    31981 aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt
    32041 tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat
    32101 ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga
    32161 catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga
    32221 ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac
    32281 ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca
    32341 tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa
    32401 agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc
    32461 tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat
    32521 tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga
    32581 attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt
    32641 cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta
    32701 taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca
    32761 gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag
    32821 gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct tctatgctag
    32881 atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga
    32941 tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt
    33001 ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc
    33061 tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg
    33121 cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa
    33181 tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg
    33241 aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt
    33301 taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa
    33361 tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc
    33421 gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga
    33481 tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat
    33541 taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa
    33601 tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt
    33661 tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga
    33721 tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt
    33781 tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat
    33841 cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat
    33901 tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg
    33961 cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat
    34021 gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac
    34081 accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc
    34141 agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat
    34201 ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa
    34261 catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg
    34321 accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct
    34381 agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa
    34441 tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact
    34501 gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa
    34561 cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg
    34621 taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt
    34681 gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat
    34741 tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc
    34801 aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg
    34861 ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag
    34921 aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt
    34981 ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt
    35041 gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt
    35101 cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa
    35161 gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa
    35221 actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa
    35281 ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat
    35341 tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc
    35401 cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa
    35461 ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg
    35521 tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat
    35581 ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt
    35641 ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc
    35701 aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc
    35761 tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat
    35821 gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa
    35881 gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat
    35941 catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca
    36001 ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct
    36061 tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa
    36121 agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag
    36181 gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg
    36241 aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag
    36301 tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca
    36361 tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac
    36421 gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca
    36481 acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg
    36541 aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc
    36601 aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg
    36661 atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga
    36721 tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca
    36781 tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag
    36841 caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac
    36901 aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa
    36961 atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga
    37021 gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat
    37081 caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa
    37141 ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat
    37201 ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt
    37261 cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc
    37321 caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata
    37381 gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac
    37441 aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg
    37501 gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg
    37561 accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg
    37621 ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa
    37681 aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg
    37741 acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt
    37801 gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt
    37861 gatgaatatt gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa
    37921 gggactgtga caaatcgtgt cacagtccct tttttatagt gttttttata ggtagtattt
    37981 tttagcaact gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc
    38041 aagatccatg atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga
    38101 gttaccatgt gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc
    38161 ttgccagaat ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc
    38221 gcgtttgtct aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc
    38281 cattaatgga ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta
    38341 tttttcagct gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa
    38401 gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca
    38461 tgttttgatt gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc
    38521 tttgattttg cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc
    38581 agcccaacca gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac
    38641 taattttggc atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt
    38701 ctgttttatt gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat
    38761 gagaaaacaa gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag
    38821 gtattagatg cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt
    38881 tccaactgaa acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata
    38941 gagacgtcgg atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc
    39001 ttttttacga gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat
    39061 gtttgctagg ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc
    39121 ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa
    39181 ttgccgcgta gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg
    39241 catgtcagcg atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata
    39301 tgtctcatgg aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa
    39361 atcaacgtca tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta
    39421 gcagtgattt tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat
    39481 agttttttcg caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta
    39541 tttacgacgg attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga
    39601 aaccatgttt aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc
    39661 taagctttct ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc
    39721 tgtggttaag acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc
    39781 aaaaatatga agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt
    39841 cccaagttta tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt
    39901 agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc
    39961 actgatttca tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt
    40021 taacggtatt cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt
    40081 gatacgtaaa ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt
    40141 tgaaccggtc ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc
    40201 agctaattgt tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt
    40261 cctcactatt ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag
    40321 ctttttcaaa aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca
    40381 aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt
    40441 tgataaggat gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg
    40501 tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta
    40561 gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga
    40621 tataacagct ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca
    40681 actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt
    40741 gttcgtttgt caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta
    40801 caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt
    40861 taccatatcg ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt
    40921 tctaaatacg gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg
    40981 cttctactat atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc
    41041 cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat
    41101 tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa acgcggtatt gcagctaaca
    41161 ttatctataa tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt
    41221 ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg
    41281 gttcaaacta ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc
    41341 gttgggtagt aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa
    41401 aagaaatcat ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc
    41461 ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt
    41521 tggtcggctt ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa
    41581 agagaggaga aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg
    41641 gtatcatggc ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata
    41701 ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg
    41761 aacaagaaca agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg
    41821 gtcaccgaat caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac
    41881 gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac
    41941 ctcaaactga aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg
    42001 caaacaaaat ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc
    42061 gtttgcaagc aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag
    42121 gaattatcga ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga
    42181 tcgaagaaac agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat
    42241 tggttgaagc tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag
    42301 aaatcactga agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct
    42361 acccagtact atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg
    42421 ttcttgatta cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa
    42481 ctgatgaaga agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta
    42541 aagttatgac tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt
    42601 tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta
    42661 tcctacagat gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg
    42721 ctgctgctgt tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc
    42781 tagttattct tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta
    42841 aatcaaaagc tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc
    42901 ctactttccg tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg
    42961 aacttcactt agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg
    43021 taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta
    43081 agtttgtacg tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac
    43141 caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc
    43201 gtgaatacat tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag
    43261 ctggctatcc attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg
    43321 attcaaatga aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga
    43381 aagctaatcc agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt
    43441 atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag
    43501 ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt
    43561 atgctacaac tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc
    43621 actacgaaga tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag
    43681 ctgaataatt tcttgaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt
    43741 ttaatatagt ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta
    43801 catcatatac ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa
    43861 tggcaaaaga aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg
    43921 ttgaccatgg taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg
    43981 gcggccaagc tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa
    44041 tcacaatctc aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg
    44101 actgcccagg acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg
    44161 gagctatctt ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc
    44221 tattgtctcg tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg
    44281 ttgatgacga agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat
    44341 acgaattccc tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag
    44401 gcgacgcttc atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc
    44461 caactccaga acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa
    44521 ttactggacg tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg
    44581 acgaagttga agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg
    44641 aaatgttccg taaattgtta gactacgctg aagctggaga caacattggt gctttactac
    44701 gtggtgttgc acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca
    44761 cacctcatac aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc
    44821 atactccatt cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag
    44881 gtgttgttga attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg
    44941 aagttgaatt aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag
    45001 gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt
    45061 taaagtaaag caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg
    45121 ttccgagctt ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa
    45181 gaaacacgaa ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt
    45241 tttacaagaa aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt
    45301 actttagtga attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt
    45361 gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt
    45421 ctacttttca aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt
    45481 ttaaaagcgt atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca
    45541 aaaagaactg gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac
    45601 acaatcatcc gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca
    45661 cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag
    45721 ctagacttac catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat
    45781 ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc
    45841 actgaatctg gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta
    45901 caagtgaaaa caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg
    45961 cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct
    46021 aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa
    46081 attacagtag atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt
    46141 aaaggattcc aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt
    46201 tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa
    46261 aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt
    46321 gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa
    46381 aaatctttaa ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg
    46441 aactaaggaa tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc
    46501 acacttaacg aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc
    46561 gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt
    46621 cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct
    46681 atccgctcac cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac
    46741 agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa
    46801 gttgctgaaa acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa
    46861 gaattcaaac aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa
    46921 aaaggtaacg actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact
    46981 tctgataatg tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact
    47041 gctcttactc aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc
    47101 cagtgatcac tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg
    47161 acactcgcgc aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag
    47221 tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag
    47281 gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc
    47341 aattattcga agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa
    47401 cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga
    47461 ttttgctgaa atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca
    47521 tgctggacgt aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg
    47581 ccaatatcgc gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac
    47641 gatcgaatat gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt
    47701 gaaagcttac atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc
    47761 agaagcagat atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt
    47821 tatccacaac attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac
    47881 aagtgctcaa gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga
    47941 agttcgtatg atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca
    48001 cgaattaatc aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt
    48061 acgtggtagc gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc
    48121 tatcggacgt aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg
    48181 taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact
    48241 ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg
    48301 accttttgtc gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa
    48361 aaaagttatc aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac
    48421 catcgcagtt tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg
    48481 acacaaatta ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa
    48541 gaaaacgaaa cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag
    48601 ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc
    48661 ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat
    48721 ctgctggaat cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg
    48781 acttagatgt tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga
    48841 aacgtttccg tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata
    48901 tcacggtagt cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca
    48961 attggaatgc gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag
    49021 tatgctgagt tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct
    49081 gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt
    49141 cacacagcta aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga
    49201 aaagaattaa acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa
    49261 ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt
    49321 gctttccgtc gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg
    49381 atcaaaactc aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac
    49441 tcagaaggaa ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa
    49501 gcagatacaa catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt
    49561 ccaactaaaa aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa
    49621 aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag
    49681 cattcggtga atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg
    49741 aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa
    49801 ttttccctca caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag
    49861 gggcacctga aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag
    49921 gcgttcctga agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga
    49981 aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc
    50041 agagaattaa ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt
    50101 aatcttagat tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta
    50161 cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag
    50221 gaggccatta agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg
    50281 gtttcagaca aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct
    50341 atctatggca aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca
    50401 gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt
    50461 ttccgtttgt tggaagtagt cgaagaagca gttattatct aataacacga gattttccta
    50521 tatagattga atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt
    50581 ttaaaagtcg ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga
    50641 tctggtcgta aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg
    50701 ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca
    50761 ggagctcgtc gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt
    50821 cgtgatgata aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt
    50881 gaaaacaact tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact
    50941 tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc
    51001 actggaaaag acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa
    51061 gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct
    51121 caaggtggaa ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac
    51181 tctactggtg tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt
    51241 tctaaaaaaa ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga
    51301 atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt
    51361 aactatagct ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt
    51421 ggtgatgcgg tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc
    51481 actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt
    51541 gaaggaatgc caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta
    51601 gacaaattgg tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa
    51661 gcctttgacg gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa
    51721 gtagattatg atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca
    51781 aactctgatg aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa
    51841 ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca
    51901 aaattttcaa cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat
    51961 cgtaagttcc atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc
    52021 ggcgtgaaga aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa
    52081 tggtcatgac agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta
    52141 aacatgagtc tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga
    52201 aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc
    52261 gtgttttcct aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct
    52321 ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt
    52381 taggtatcgc aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa
    52441 aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg
    52501 tgtctttaag tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt
    52561 actcaagacg gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt
    52621 tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac
    52681 agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta
    52741 ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa
    52801 ttacaaggat caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca
    52861 ccagctggag taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac
    52921 aaagaagaag ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat
    52981 aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt
    53041 aaataatcgt ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa
    53101 gtttaccttc aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat
    53161 ttactgcttg aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc
    53221 agataagaac aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac
    53281 tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat
    53341 tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg
    53401 tggaacaaaa acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga
    53461 aaaaggcatt aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca
    53521 agctttagct gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca
    53581 ttcatggttt atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac
    53641 cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc
    53701 ggtgacaaaa acggacacgt agggtttggt acaggtaaag ctcaagaagt tcctgaagca
    53761 atccgtaaag caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca
    53821 acaatcccac acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct
    53881 gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg
    53941 gtagcagata tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca
    54001 acagttgaag gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa
    54061 tcagttgaag aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt
    54121 taaaacgcag tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc
    54181 ttacaaagat taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca
    54241 atactgtttc tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa
    54301 cattaaggag gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc
    54361 caagtacgta accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc
    54421 ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga
    54481 caaacaccat tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac
    54541 tatgcagtca ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct
    54601 gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct
    54661 aatggggaac taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa
    54721 gaagcaattg aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag
    54781 atgcttttaa agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg
    54841 tatttcgcct aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc
    54901 tttcttcttt gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact
    54961 tttccatttt ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc
    55021 ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta
    55081 aaaaactgaa tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg
    55141 gtatcactgc tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag
    55201 caacttacgt aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag
    55261 gagaacagat cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta
    55321 tcatttctag attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg
    55381 atccttcaga catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt
    55441 tgatcgtgac attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata
    55501 cgaaacgcgt agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg
    55561 gagttattcc agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag
    55621 cattgagcgg tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact
    55681 ataatacagc acctggtgct acgatttata cgattctgat cgttgccttc actttcttct
    55741 acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt
    55801 atataccaag cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc
    55861 gtttaagtac agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc
    55921 aaatggtttg ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta
    55981 tcggtgtagc tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg
    56041 ttggattcat tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat
    56101 ttattaggag ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga
    56161 acacaagctg aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg
    56221 ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat
    56281 aaaggtgcat tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa
    56341 ccagatacag agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa
    56401 gctctggacg caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat
    56461 gttggtgaag aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga
    56521 gcaacttacc ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt
    56581 gggcatgaat tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct
    56641 atcaacatca aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac
    56701 actattgatg gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa
    56761 aaatagcacg attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata
    56821 aattatgata gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag
    56881 aattttgagg tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta
    56941 caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga
    57001 aactttgccg aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac
    57061 tgtttcaggt aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt
    57121 tgaattgtct ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta
    57181 ctccgtaaaa ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc
    57241 gaacattgta aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca
    57301 aaacataaac aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag
    57361 tagatattcc tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta
    57421 acactactgc taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg
    57481 aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag
    57541 gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc
    57601 gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac
    57661 gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag
    57721 gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata
    57781 tagaagcagg tattgcacat atccattcta cattcaataa cactatcatc atgatcactg
    57841 atactcacgg aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca
    57901 aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg
    57961 aacacggatt aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag
    58021 caattcgttc attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag
    58081 ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt
    58141 tgagttttat cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata
    58201 agaatgattg agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc
    58261 aagttcatcg ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt
    58321 cgtattttat tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta
    58381 ctgcatgaat tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac
    58441 attaaaggct tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt
    58501 accggacctg ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt
    58561 aacaaagata tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca
    58621 gtgaaacctg gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt
    58681 ggcgttcttc cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag
    58741 aacacacgtg ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat
    58801 ggttcaattg aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg
    58861 gatatttttg tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa
    58921 gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc
    58981 tcttacaatt gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct
    59041 gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca
    59101 aaattgcacg acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg
    59161 gaaaccaacg tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg
    59221 cgtgatttaa caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg
    59281 aaagaagttc gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat
    59341 gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat
    59401 gaggacatcg tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac
    59461 gcagaacgtc aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt
    59521 gcaccaatgg ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg
    59581 attcttttag agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc
    59641 ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca
    59701 attacctgtc atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat
    59761 tgaaaatttt gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat
    59821 catggagcca attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg
    59881 ccccgcattg aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg
    59941 acacaatggt tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga
    60001 atcagggatc gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg
    60061 acagatggtt ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga
    60121 agatgacgtt gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg
    60181 tgtgaaagat gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg
    60241 tttatcagga gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga
    60301 tatcattatt ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat
    60361 ctctacgatc aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga
    60421 tattgatgaa gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg
    60481 tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga
    60541 tctaccattt cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag
    60601 tgaatatatg acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta
    60661 gacttcactt atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg
    60721 acgatcaaag aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc
    60781 ttacttcagc atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa
    60841 cgagatattc agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc
    60901 atcgtctttc agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct
    60961 ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca
    61021 ttagaacaag ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga
    61081 gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta
    61141 ttggatgaac caacggcagg acttgatccc cagggtagaa aagaaatgat ggagatgttt
    61201 tggcgtcttc ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat
    61261 gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg
    61321 gagccgcaag aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct
    61381 actgccaccg agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct
    61441 ttgacagctg aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat
    61501 gaataaattg atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc
    61561 ccgagctaaa ttattagcca gtttttattt tatcggaatc atttttttag caaataattg
    61621 ggaaacgtac cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga
    61681 tttgaaattt tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc
    61741 tttgcaggtc ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat
    61801 cactgaattt ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt
    61861 tatgtcgact ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta
    61921 cttgcttcgt ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc
    61981 gattgctctt cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca
    62041 gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat
    62101 tcctttgctg attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc
    62161 tatggaagca agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg
    62221 gcataataga gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt
    62281 tttgagagga taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca
    62341 agaaaggatg cgagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac
    62401 aggcctcttt cttttatctg aaaagaaaga acgaaaaaat tttataaaaa aatcattatt
    62461 ccagataacc ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg
    62521 catacgacgg aacaaatttt aatggattcc aaaaacagcc aaatgggcgt actgttcaag
    62581 aagaagtaga aaaaacatta caaaaaatgg caaatggaaa agaaatcacg gtctttggtt
    62641 cgggaaggac agatgccgga gtacatgcga ttgggcaagt aatccatttt gattatccag
    62701 aggagcgtcc gttagaacga atgcgtttcg cgctagatac ccagtcacca gaggatattg
    62761 ctgtaagaca agtagaaatt gtttcagaag acttccacgc aagatatttg gtcaaagaaa
    62821 agacgtatca atttcgagtg gatataggga aaccacgcag tccttttcga cgccattatg
    62881 caagttattt tccttaccct cttgatttgg aaaaaattca gcgggcgctt cctgatttat
    62941 tagggactca cgatttcacc tctttttgtg catcaggaag ctcgatagag gataaagtac
    63001 gtacgatcta cgaggctaag atggaggtaa atgaaacaaa agacgaattg ttgtttacat
    63061 ttaggggaaa tggcttcttg tataagatga tacggatctt agtaggaacg cttttgaaaa
    63121 ttggcaatgg ccgattgcct gaagatagta tcccagatat tattgcgaaa aaagatcgta
    63181 atgcagcagg acctacagca catcctgaag gactttattt gtatgaagtg aaatatgaat
    63241 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattact
    63301 tctcaaaatt ttgcaaaatt tcggcttatt ttcgaaggag ctacttctgg aacaccgtga
    63361 atcagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccaa acaaaaatag
    63421 ctcgtcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    63481 ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggaaaa
    63541 atagttcctc atattttgcc gtttgttgag ctaataccga aggtcagctg attgaacacc
    63601 gtttaagaaa gaggtcgtga gcctgtcatt taactctgag tcacaagtaa caatttcaaa
    63661 aaacagcttc tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat
    63721 catctttttg ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc
    63781 ccttaatgag gacagtcgga acaggtttgt catttttata tttaaatggc acaaattttt
    63841 ctaattattt gtcatttagc gctgaaaatt tttgctattt atctaaaaaa gtgataacta
    63901 cttaagatga agcagaatat ttacgccata cggcatacaa ccgcgaaaaa tataaaaaaa
    63961 agaaaacgat tgaacgcgtt tttgcagtcg caaaggaaaa gcatggaatg cgctacatca
    64021 agtatcgaga cttggaaaac aatcaggtgt atacgctgat tgtttttgct gcaatgaatc
    64081 tgaaaaagtt gtccacttgg cagttggaac atatgtcttc atatctttta ttatacaaaa
    64141 atagcaaaag caccagtcta cactcttagt aaagaggaaa ctgatccttt tgtctacatt
    64201 cttaaagtac ttaaactaag tactttattt tactctttag gattgataca gtagcatcta
    64261 tccctttatt aataatcttt tattttttta aataaagatg ttgtctccat cttcaggcac
    64321 taaaactttt tcggaaattc ctttttcttt attgaaagac tttaattctt ctcttgacag
    64381 tccccaatgg tttacagctt ccatatgaac ggcaataatt gtggaatctg gtattgcttt
    64441 atgcaattca tataaatcgt ctttccccat aataagagat tctccaacta aaaaatgatt
    64501 atctccagca tttgctacta tgatttctgg attatactga tcaatagttt gtttaacagc
    64561 ttcataccaa actgtatctc ctgtaaagta tagtgtcttc tcagtcgaat gtttgaaaac
    64621 aataccgcaa acattaccta cataattcaa gatttctcct ctgccatgtt tgccttttgt
    64681 tttaaccaat tgtatagatt caaaaactgt gtcttctcgt agtacctcca catttttaaa
    64741 accatatagt cgaatttcat tcgcatcatt ttcatcttgc acaaataatt ttatttcttt
    64801 aggtaggacc tctttcgcta tatcgtccca atgatctaga tgtaaatgtg ttacaataac
    64861 agcatcaata ccgttgataa tatcttcgac tggcattggc aaatcaacta gaggattcat
    64921 ttgatctttt cttggtgatg gaaaaggacc tttcgaaata aatggtggaa gtgttccttt
    64981 ttccgctaac attggatcaa ttaaaaattt tttacctcca tagtctaaga tagctgttgc
    65041 attacgaatg tgatgtatat tcataaacgt tgtctccttt ttgttgatat gaaatatttt
    65101 tagtataatt caattccata caataaataa accgaacaaa aaaagtcttt tactattttt
    65161 acttaattta tgaaaggttg tttaccatgt tagaccaaac agatataaat atcattgagg
    65221 aactatcaaa aaatagtcga atcacaatga aagaacttgg agaaaaagta catttgactg
    65281 gacccgctgt ctcggcgaga gtgacaaaac tagaggaaag tggagttatt gaaaattata
    65341 cagtaaaatt aaatatggaa aagctagggt tttcgattca cgctttttta attataatta
    65401 cgcaaaattt aaatcataaa ccctatttat catttattaa aaatcatgaa aaattcttga
    65461 ttagaaatta taagattagt ggagggggct gttatctttt ggaatgtaga tttacttcaa
    65521 aaaaatcaat gaacagcttt ttggaacagc ttaatgaata tgcgaattat aaattatcta
    65581 ttgttataga ttaataataa aactagcaga tggtaaaaat actaatatgc gagacacaca
    65641 ttcaaccata actatataac ttatcaattt aaaactaaac tgatggaaac aagtaaggca
    65701 aaaacattta tattttgccc tacttatttt tttaattaaa ttcattgatg attcatattt
    65761 agaaaattct atgaaggcca aatgagtatc ctgtcacttt tttagataaa tagcaaaaat
    65821 tttcagcgtt aaatgacaaa taattagaaa tatttgtgcc atttaaatat aaaaatgaca
    65881 ccaagtatat catgaacgca caaaaagacc aacagggtat accctgttgg tctaaa
//
LOCUS       DANKHJ010000006        64222 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000006 DANKHJ010000000
VERSION     DANKHJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(151..2304)
                     /locus_tag="PW113_000487"
     CDS             complement(151..2304)
                     /locus_tag="PW113_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2524823.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="PW113_000488"
     CDS             complement(2598..3446)
                     /locus_tag="PW113_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2524824.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PW113_000489"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PW113_000489"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2524825.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="PW113_000490"
     CDS             complement(5947..6126)
                     /locus_tag="PW113_000490"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2524826.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PW113_000491"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PW113_000491"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2524827.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="PW113_000492"
     CDS             8274..10553
                     /locus_tag="PW113_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2524828.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="PW113_000493"
     CDS             complement(10646..11293)
                     /locus_tag="PW113_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524829.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="PW113_000494"
     CDS             complement(11472..12191)
                     /locus_tag="PW113_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2524830.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="PW113_000495"
     CDS             complement(12322..13473)
                     /locus_tag="PW113_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2524831.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="PW113_000496"
     CDS             complement(13690..14430)
                     /locus_tag="PW113_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2524832.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PW113_000497"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PW113_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2524833.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PW113_000498"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PW113_000498"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2524834.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="PW113_000499"
     CDS             complement(15391..16041)
                     /locus_tag="PW113_000499"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2524835.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PW113_000500"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PW113_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2524836.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PW113_000501"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PW113_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2524837.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="PW113_000502"
     CDS             complement(17484..18011)
                     /locus_tag="PW113_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2524838.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="PW113_000503"
     CDS             complement(18191..18976)
                     /locus_tag="PW113_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2524839.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="PW113_000504"
     CDS             complement(18969..19838)
                     /locus_tag="PW113_000504"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2524840.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="PW113_000505"
     CDS             complement(19839..21218)
                     /locus_tag="PW113_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2524841.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PW113_000506"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PW113_000506"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2524842.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PW113_000507"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PW113_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2524843.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PW113_000508"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PW113_000508"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2524844.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PW113_000509"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PW113_000509"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2524845.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PW113_000510"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PW113_000510"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2524846.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="PW113_000511"
     CDS             complement(25176..25400)
                     /locus_tag="PW113_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2524847.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="PW113_000512"
     CDS             complement(25453..26415)
                     /locus_tag="PW113_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2524848.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="PW113_000513"
     CDS             complement(26425..26865)
                     /locus_tag="PW113_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2524849.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="PW113_000514"
     CDS             27171..28136
                     /locus_tag="PW113_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2524850.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="PW113_000515"
     CDS             complement(28193..28423)
                     /locus_tag="PW113_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2524851.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="PW113_000516"
     CDS             complement(28509..29396)
                     /locus_tag="PW113_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2524852.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="PW113_000517"
     CDS             complement(29485..30708)
                     /locus_tag="PW113_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2524853.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="PW113_000518"
     CDS             complement(30725..31771)
                     /locus_tag="PW113_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2524854.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="PW113_000519"
     CDS             complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="PW113_000519"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2524855.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33662..33928)
                     /locus_tag="PW113_000520"
     CDS             complement(33662..33928)
                     /locus_tag="PW113_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2524856.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34407..36641
                     /locus_tag="PW113_000521"
     CDS             34407..36641
                     /locus_tag="PW113_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2524857.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36789..37109
                     /locus_tag="PW113_000522"
     CDS             36789..37109
                     /locus_tag="PW113_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2524858.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37314..37538)
                     /locus_tag="PW113_000523"
     CDS             complement(37314..37538)
                     /locus_tag="PW113_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2524859.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37736..39316)
                     /locus_tag="PW113_000524"
     CDS             complement(37736..39316)
                     /locus_tag="PW113_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2524860.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39717..41093)
                     /locus_tag="PW113_000525"
     CDS             complement(39717..41093)
                     /locus_tag="PW113_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2524861.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41221..42339)
                     /locus_tag="PW113_000526"
     CDS             complement(41221..42339)
                     /locus_tag="PW113_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2524862.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42481..42915
                     /locus_tag="PW113_000527"
     CDS             42481..42915
                     /locus_tag="PW113_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2524863.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42993..43526)
                     /locus_tag="PW113_000528"
     CDS             complement(42993..43526)
                     /locus_tag="PW113_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2524864.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="PW113_000529"
     CDS             complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="PW113_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2524865.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44791..45588)
                     /gene="recX"
                     /locus_tag="PW113_000530"
     CDS             complement(44791..45588)
                     /gene="recX"
                     /locus_tag="PW113_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2524866.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45695..47077
                     /gene="rlmD"
                     /locus_tag="PW113_000531"
     CDS             45695..47077
                     /gene="rlmD"
                     /locus_tag="PW113_000531"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2524867.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47251..47829)
                     /locus_tag="PW113_000532"
     CDS             complement(47251..47829)
                     /locus_tag="PW113_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2524868.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48151..48621
                     /locus_tag="PW113_000533"
     CDS             48151..48621
                     /locus_tag="PW113_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2524869.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48729..49955)
                     /locus_tag="PW113_000534"
     CDS             complement(48729..49955)
                     /locus_tag="PW113_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2524870.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50709..50909)
                     /locus_tag="PW113_000535"
     CDS             complement(50709..50909)
                     /locus_tag="PW113_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2524871.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51043..51651)
                     /locus_tag="PW113_000536"
     CDS             complement(51043..51651)
                     /locus_tag="PW113_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2524872.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51736..52206)
                     /locus_tag="PW113_000537"
     CDS             complement(51736..52206)
                     /locus_tag="PW113_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2524873.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52254..53168)
                     /locus_tag="PW113_000538"
     CDS             complement(52254..53168)
                     /locus_tag="PW113_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2524874.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53314..54117
                     /locus_tag="PW113_000539"
     CDS             53314..54117
                     /locus_tag="PW113_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2524875.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54295..55242
                     /locus_tag="PW113_000540"
     CDS             54295..55242
                     /locus_tag="PW113_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2524876.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55310..55603)
                     /locus_tag="PW113_000541"
     CDS             complement(55310..55603)
                     /locus_tag="PW113_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2524877.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(55631..56296)
                     /locus_tag="PW113_000542"
     CDS             complement(55631..56296)
                     /locus_tag="PW113_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2524878.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56436..57068)
                     /locus_tag="PW113_000543"
     CDS             complement(56436..57068)
                     /locus_tag="PW113_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2524879.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(57075..58142)
                     /locus_tag="PW113_000544"
     CDS             complement(57075..58142)
                     /locus_tag="PW113_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2524880.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(58139..58870)
                     /locus_tag="PW113_000545"
     CDS             complement(58139..58870)
                     /locus_tag="PW113_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2524881.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(59039..59518)
                     /gene="greA"
                     /locus_tag="PW113_000546"
     CDS             complement(59039..59518)
                     /gene="greA"
                     /locus_tag="PW113_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2524882.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="PW113_000547"
     CDS             complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="PW113_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2524883.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            61033..62202
                     /locus_tag="PW113_000548"
     CDS             61033..62202
                     /locus_tag="PW113_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2524884.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(62469..64103)
                     /locus_tag="PW113_000549"
     CDS             complement(62469..64103)
                     /locus_tag="PW113_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524885.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        18743 a        13368 c        10773 g        21338 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca ataagcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    31861 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    31921 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    31981 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    32041 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    32101 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    32161 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    32221 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    32281 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    32341 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    32401 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    32461 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    32521 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    32581 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    32641 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    32701 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    32761 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    32821 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    32881 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    32941 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    33001 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    33061 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    33121 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    33181 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    33241 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    33301 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    33361 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    33421 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    33481 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    33541 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    33601 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    33661 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    33721 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    33781 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    33841 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    33901 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    33961 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    34021 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    34081 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    34141 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    34201 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    34261 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    34321 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    34381 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    34441 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    34501 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    34561 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    34621 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    34681 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    34741 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    34801 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    34861 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    34921 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    34981 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    35041 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    35101 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    35161 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    35221 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    35281 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    35341 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    35401 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    35461 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    35521 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    35581 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    35641 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    35701 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    35761 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    35821 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    35881 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    35941 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    36001 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    36061 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    36121 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    36181 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    36241 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    36301 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    36361 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    36421 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    36481 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    36541 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    36601 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    36661 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    36721 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    36781 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    36841 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    36901 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    36961 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    37021 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    37081 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    37141 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    37201 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    37261 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    37321 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    37381 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    37441 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    37501 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    37561 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    37621 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    37681 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    37741 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    37801 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    37861 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    37921 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    37981 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    38041 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    38101 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    38161 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    38221 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    38281 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    38341 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    38401 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    38461 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    38521 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    38581 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    38641 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    38701 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    38761 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    38821 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    38881 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    38941 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    39001 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    39061 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    39121 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    39181 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    39241 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    39301 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    39361 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    39421 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    39481 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    39541 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    39601 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    39661 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    39721 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    39781 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    39841 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    39901 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    39961 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    40021 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    40081 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    40141 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    40201 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    40261 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    40321 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    40381 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    40441 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    40501 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    40561 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    40621 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    40681 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    40741 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    40801 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    40861 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    40921 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    40981 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    41041 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    41101 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    41161 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    41221 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    41281 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    41341 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    41401 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    41461 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    41521 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    41581 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    41641 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    41701 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    41761 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    41821 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    41881 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    41941 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    42001 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    42061 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    42121 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    42181 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    42241 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    42301 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    42361 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    42421 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    42481 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    42541 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    42601 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    42661 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    42721 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    42781 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    42841 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    42901 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    42961 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    43021 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    43081 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    43141 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    43201 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    43261 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    43321 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    43381 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    43441 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    43501 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    43561 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    43621 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    43681 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    43741 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    43801 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    43861 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    43921 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    43981 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    44041 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    44101 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    44161 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    44221 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    44281 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    44341 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    44401 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    44461 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    44521 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    44581 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    44641 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    44701 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    44761 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    44821 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    44881 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    44941 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    45001 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    45061 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    45121 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    45181 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    45241 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    45301 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    45361 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    45421 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    45481 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    45541 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    45601 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    45661 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    45721 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    45781 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    45841 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    45901 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    45961 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    46021 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    46081 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    46141 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    46201 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    46261 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    46321 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    46381 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    46441 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    46501 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    46561 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    46621 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    46681 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    46741 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    46801 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    46861 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    46921 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    46981 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    47041 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    47101 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    47161 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    47221 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    47281 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    47341 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    47401 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    47461 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    47521 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    47581 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    47641 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    47701 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    47761 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    47821 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    47881 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    47941 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    48001 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    48061 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    48121 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    48181 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    48241 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    48301 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    48361 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    48421 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    48481 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    48541 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    48601 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    48661 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    48721 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    48781 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    48841 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    48901 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    48961 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    49021 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    49081 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    49141 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    49201 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    49261 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    49321 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    49381 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    49441 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    49501 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    49561 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    49621 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    49681 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    49741 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    49801 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    49861 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    49921 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    49981 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    50041 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    50101 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    50161 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    50221 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    50281 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    50341 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    50401 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    50461 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    50521 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    50581 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    50641 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    50701 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    50761 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    50821 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    50881 ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa
    50941 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    51001 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    51061 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    51121 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    51181 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    51241 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    51301 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    51361 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    51421 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    51481 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt
    51541 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    51601 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    51661 cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    51721 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    51781 agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg
    51841 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    51901 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    51961 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    52021 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    52081 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    52141 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    52201 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    52261 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    52321 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    52381 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    52441 ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca
    52501 atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca
    52561 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    52621 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    52681 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    52741 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    52801 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    52861 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    52921 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    52981 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    53041 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    53101 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    53161 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    53221 accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt
    53281 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    53341 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    53401 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    53461 gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    53521 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    53581 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    53641 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    53701 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    53761 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    53821 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    53881 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    53941 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    54001 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    54061 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    54121 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    54181 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    54241 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    54301 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    54361 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    54421 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    54481 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    54541 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    54601 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    54661 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    54721 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    54781 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    54841 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    54901 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    54961 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    55021 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    55081 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    55141 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    55201 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    55261 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    55321 taaagtgata ggttggctcc tccaggttgg gattataatc aacaagtaaa tcatatcctt
    55381 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct
    55441 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    55501 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    55561 agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    55621 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    55681 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    55741 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    55801 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    55861 tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta
    55921 ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg
    55981 catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca
    56041 gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat
    56101 ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag
    56161 tggctatatt catatattca ttgacccgat cttcattact atcaatagcc agtacttctt
    56221 gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg
    56281 caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc
    56341 tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    56401 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat
    56461 gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca
    56521 tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt
    56581 ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca
    56641 ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact
    56701 cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc
    56761 aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa
    56821 ctggttacga taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg
    56881 ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc
    56941 gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga
    57001 atagataaat aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact
    57061 tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct
    57121 tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    57181 ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca
    57241 ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct
    57301 ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    57361 atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt
    57421 tgcaattcct tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact
    57481 ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct
    57541 accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc
    57601 aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca
    57661 cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat
    57721 tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc
    57781 ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga
    57841 agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt
    57901 ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct
    57961 aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat
    58021 tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata
    58081 ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc
    58141 atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag
    58201 ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct
    58261 cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca
    58321 aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    58381 ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga
    58441 tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt
    58501 tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc
    58561 gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa
    58621 ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    58681 tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca
    58741 tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta
    58801 cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag
    58861 aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt
    58921 aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag
    58981 agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact
    59041 acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta
    59101 cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt
    59161 tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat
    59221 ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt
    59281 cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa
    59341 caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac
    59401 cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta
    59461 attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt
    59521 gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc
    59581 aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct
    59641 agagcctgtt tttctaagaa ttatttacat atttttccac taattccata tgttcttcat
    59701 aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat
    59761 tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag
    59821 gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg
    59881 tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca
    59941 tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta
    60001 cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct
    60061 gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca
    60121 ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt
    60181 ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt
    60241 ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt
    60301 taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg
    60361 caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc
    60421 caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta
    60481 aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct
    60541 ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt
    60601 ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc
    60661 caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa
    60721 tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct
    60781 tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc
    60841 cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat
    60901 tttatttaaa tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata
    60961 aatgttatca agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact
    61021 aaataaacta ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg
    61081 atcgttgtcg tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa
    61141 ttttacaata atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg
    61201 atactgacta ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat
    61261 gattatattg gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat
    61321 ggctatgcga ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt
    61381 tcggttcaaa aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt
    61441 tctttagata ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact
    61501 atccgtgact tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg
    61561 atgactgatg aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag
    61621 acctctcatt atgattcatt gaactttacc atattgactg gaattttgat caaattaaca
    61681 aatcaaccgt atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat
    61741 acaaaatttt atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct
    61801 aaagaggatg attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta
    61861 gggactggta atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta
    61921 tctggaaaac tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga
    61981 catccatata gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc
    62041 gactcttttg atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa
    62101 agcaatataa aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc
    62161 tatgacctga tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca
    62221 aatactactt ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga
    62281 gtgtgggaca aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac
    62341 agaaagaggt gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac
    62401 attatttgac aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg
    62461 ctagcaaatc attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca
    62521 attagcgtga ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg
    62581 attgttactt tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt
    62641 agtactgtag cattttttga attaacagta accgttaatc ctttataccg gaatacaggt
    62701 aaaactattg ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga
    62761 gaaaagctac tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat
    62821 tgatcttttt tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt
    62881 tccatttgtt cattagaata agttccttga aaattcattt ctttcaccat gttaactact
    62941 gatgaataat ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt
    63001 gtataagaaa atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac
    63061 aatgttgaaa atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt
    63121 aaggcaaacc atggaaatat ttttgtacaa agtagaatag atattatacc cattatataa
    63181 gtgattttag ataagattcc taatttttta aagttaaatg ttagtataat taaacaaata
    63241 agcaaagtta agccaatacc aaatgatagt aattctgaat ctatacttcc aatcaaaaac
    63301 tctttaccca gataaattgc tcttttttgt aatatatcca cataaggtct attcagatct
    63361 tgatacaatg tctgctcaat cataggacct ataaacgcta gtgaaagaag taaagtaact
    63421 atagcagctt ttattaattt aataattaag aagatttcta ttttcttttt ccgataaaat
    63481 tgaataatta aagcaattat aatcattacc atagtcatag ctacagaaag aacatgagtg
    63541 taaattaata aagtcatgct aatcgccaac ataacccaag attctttttt tgagtcgtaa
    63601 agctttataa acccgagtaa aataagagga agtattgaca ttgcgagtaa ttcccccatt
    63661 gcccctcgaa gaaatatttc tacactccta aaagaagatg ttgtatatat aatagcgaaa
    63721 aaggagctag taacatgttt tttggtaatt tcatacatta cataatgaca tataaataga
    63781 gtaatgagcg taacaatata aagaaaaagg ttatagccta ttgctaaatt agtaaacttg
    63841 atcaataaaa ataaaggata tagagttaac catgggtaaa aattatttac ttgcatgcca
    63901 tatgatctaa acgtttcaaa attaataggg gagctaaaaa tagattctaa acttaacgca
    63961 cgattaatgt gaaatgccgt atcataaaag ccaaatattt ccatgttttt aaatgctgga
    64021 aataaatata ataaactaac aaatgcataa actataaata tttttacaga aaaaattttt
    64081 ttcaaattca tataccacct catatttttt tatattacat atagtatcat ttattttttt
    64141 taaacactag attagtcact atataaaatg tgattaattt tattattaat aaaatatttt
    64201 ttatattaca aaagtagtgt tt
//
LOCUS       DANKHJ010000007        62775 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000007 DANKHJ010000000
VERSION     DANKHJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            76..1017
                     /locus_tag="PW113_000550"
     CDS             76..1017
                     /locus_tag="PW113_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524886.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            1018..2061
                     /locus_tag="PW113_000551"
     CDS             1018..2061
                     /locus_tag="PW113_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524887.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            2067..3137
                     /locus_tag="PW113_000552"
     CDS             2067..3137
                     /locus_tag="PW113_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524888.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            3127..4080
                     /locus_tag="PW113_000553"
     CDS             3127..4080
                     /locus_tag="PW113_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2524889.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            4092..5102
                     /locus_tag="PW113_000554"
     CDS             4092..5102
                     /locus_tag="PW113_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2524890.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5387..6208)
                     /locus_tag="PW113_000555"
     CDS             complement(5387..6208)
                     /locus_tag="PW113_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2524891.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(6305..6583)
                     /locus_tag="PW113_000556"
     CDS             complement(6305..6583)
                     /locus_tag="PW113_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524892.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            6851..8860
                     /gene="metG"
                     /locus_tag="PW113_000557"
     CDS             6851..8860
                     /gene="metG"
                     /locus_tag="PW113_000557"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2524893.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(9339..9926)
                     /locus_tag="PW113_000558"
     CDS             complement(9339..9926)
                     /locus_tag="PW113_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524894.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            10101..11423
                     /locus_tag="PW113_000559"
     CDS             10101..11423
                     /locus_tag="PW113_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2524895.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            11578..12354
                     /locus_tag="PW113_000560"
     CDS             11578..12354
                     /locus_tag="PW113_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2524896.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            12347..12916
                     /gene="rnmV"
                     /locus_tag="PW113_000561"
     CDS             12347..12916
                     /gene="rnmV"
                     /locus_tag="PW113_000561"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2524897.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            12909..13793
                     /gene="rsmA"
                     /locus_tag="PW113_000562"
     CDS             12909..13793
                     /gene="rsmA"
                     /locus_tag="PW113_000562"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2524898.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            14244..14873
                     /locus_tag="PW113_000563"
     CDS             14244..14873
                     /locus_tag="PW113_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524899.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(14997..16106)
                     /gene="ugpC"
                     /locus_tag="PW113_000564"
     CDS             complement(14997..16106)
                     /gene="ugpC"
                     /locus_tag="PW113_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2524900.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            16315..16737
                     /gene="mgsA"
                     /locus_tag="PW113_000565"
     CDS             16315..16737
                     /gene="mgsA"
                     /locus_tag="PW113_000565"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2524901.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            17060..17596
                     /locus_tag="PW113_000566"
     CDS             17060..17596
                     /locus_tag="PW113_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2524902.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(17636..19399)
                     /locus_tag="PW113_000567"
     CDS             complement(17636..19399)
                     /locus_tag="PW113_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524903.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(19402..21117)
                     /locus_tag="PW113_000568"
     CDS             complement(19402..21117)
                     /locus_tag="PW113_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524904.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(21896..22120)
                     /locus_tag="PW113_000569"
     CDS             complement(21896..22120)
                     /locus_tag="PW113_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524905.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            22311..25088
                     /locus_tag="PW113_000570"
     CDS             22311..25088
                     /locus_tag="PW113_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2524906.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            25169..25588
                     /locus_tag="PW113_000571"
     CDS             25169..25588
                     /locus_tag="PW113_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2524907.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            25600..26070
                     /locus_tag="PW113_000572"
     CDS             25600..26070
                     /locus_tag="PW113_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2524908.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            26087..26866
                     /locus_tag="PW113_000573"
     CDS             26087..26866
                     /locus_tag="PW113_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2524909.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            26853..27674
                     /locus_tag="PW113_000574"
     CDS             26853..27674
                     /locus_tag="PW113_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2524910.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            27691..28740
                     /locus_tag="PW113_000575"
     CDS             27691..28740
                     /locus_tag="PW113_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2524911.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            28753..29757
                     /locus_tag="PW113_000576"
     CDS             28753..29757
                     /locus_tag="PW113_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2524912.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            30390..31409
                     /locus_tag="PW113_000577"
     CDS             30390..31409
                     /locus_tag="PW113_000577"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2524913.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            31452..32333
                     /gene="galU"
                     /locus_tag="PW113_000578"
     CDS             31452..32333
                     /gene="galU"
                     /locus_tag="PW113_000578"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2524914.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            32633..33010
                     /locus_tag="PW113_000579"
     CDS             32633..33010
                     /locus_tag="PW113_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524915.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            33616..34230
                     /locus_tag="PW113_000580"
     CDS             33616..34230
                     /locus_tag="PW113_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2524916.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            35003..36742
                     /gene="yfcC"
                     /locus_tag="PW113_000581"
     CDS             35003..36742
                     /gene="yfcC"
                     /locus_tag="PW113_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2524917.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            36899..38083
                     /gene="iadA"
                     /locus_tag="PW113_000582"
     CDS             36899..38083
                     /gene="iadA"
                     /locus_tag="PW113_000582"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2524918.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            38194..38397
                     /locus_tag="PW113_000583"
     CDS             38194..38397
                     /locus_tag="PW113_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2524919.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(38498..39160)
                     /locus_tag="PW113_000584"
     CDS             complement(38498..39160)
                     /locus_tag="PW113_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2524920.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(39173..40675)
                     /locus_tag="PW113_000585"
     CDS             complement(39173..40675)
                     /locus_tag="PW113_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2524921.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            41223..42902
                     /locus_tag="PW113_000586"
     CDS             41223..42902
                     /locus_tag="PW113_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2524922.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            43029..43508
                     /locus_tag="PW113_000587"
     CDS             43029..43508
                     /locus_tag="PW113_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2524923.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            43727..44407
                     /locus_tag="PW113_000588"
     CDS             43727..44407
                     /locus_tag="PW113_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2524924.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            44415..45746
                     /locus_tag="PW113_000589"
     CDS             44415..45746
                     /locus_tag="PW113_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2524925.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            45761..46723
                     /locus_tag="PW113_000590"
     CDS             45761..46723
                     /locus_tag="PW113_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2524926.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRLDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            46834..47172
                     /locus_tag="PW113_000591"
     CDS             46834..47172
                     /locus_tag="PW113_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2524927.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            47251..47958
                     /locus_tag="PW113_000592"
     CDS             47251..47958
                     /locus_tag="PW113_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2524928.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(48061..49542)
                     /locus_tag="PW113_000593"
     CDS             complement(48061..49542)
                     /locus_tag="PW113_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2524929.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            49851..50387
                     /locus_tag="PW113_000594"
     CDS             49851..50387
                     /locus_tag="PW113_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2524930.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            50465..51835
                     /locus_tag="PW113_000595"
     CDS             50465..51835
                     /locus_tag="PW113_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2524931.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            51902..52237
                     /locus_tag="PW113_000596"
     CDS             51902..52237
                     /locus_tag="PW113_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524932.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(52318..53175)
                     /locus_tag="PW113_000597"
     CDS             complement(52318..53175)
                     /locus_tag="PW113_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2524933.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            53281..53796
                     /gene="tadA"
                     /locus_tag="PW113_000598"
     CDS             53281..53796
                     /gene="tadA"
                     /locus_tag="PW113_000598"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2524934.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            53819..53903
                     /gene="ffs"
                     /locus_tag="PW113_000599"
     ncRNA           53819..53903
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PW113_000599"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            54113..55498
                     /locus_tag="PW113_000600"
     CDS             54113..55498
                     /locus_tag="PW113_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2524935.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            55535..56212
                     /locus_tag="PW113_000601"
     CDS             55535..56212
                     /locus_tag="PW113_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2524936.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            56220..56984
                     /locus_tag="PW113_000602"
     CDS             56220..56984
                     /locus_tag="PW113_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2524937.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            56986..57645
                     /locus_tag="PW113_000603"
     CDS             56986..57645
                     /locus_tag="PW113_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2524938.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(57675..58184)
                     /locus_tag="PW113_000604"
     CDS             complement(57675..58184)
                     /locus_tag="PW113_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2524939.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(58296..59294)
                     /locus_tag="PW113_000605"
     CDS             complement(58296..59294)
                     /locus_tag="PW113_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2524940.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            59495..60079
                     /locus_tag="PW113_000606"
     CDS             59495..60079
                     /locus_tag="PW113_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2524941.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(60156..61349)
                     /locus_tag="PW113_000607"
     CDS             complement(60156..61349)
                     /locus_tag="PW113_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2524942.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            61413..61793
                     /locus_tag="PW113_000608"
     CDS             61413..61793
                     /locus_tag="PW113_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2524943.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            61867..62229
                     /locus_tag="PW113_000609"
     CDS             61867..62229
                     /locus_tag="PW113_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524944.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT        20473 a        10802 c        12917 g        18583 t
ORIGIN      
        1 tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga agtcaaaagc
       61 tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt ctttttcatg
      121 atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt gctgccgggc
      181 acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct aaataaacaa
      241 gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa tttgcttcaa
      301 ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt gttagctgga
      361 agaatcggac catccttgca gttagggtta caggcaatta tctttggaac agtcctaggg
      421 acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac atcttctaca
      481 ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt attacaatat
      541 atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt tgtgtacaca
      601 atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg gtttatccgt
      661 acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc aaaaggattg
      721 agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc attgatgact
      781 ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga aaatatcttt
      841 gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga ttatcctacg
      901 atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt gatcgtggat
      961 ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg aggataaatg
     1021 gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga atttcttcca
     1081 ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt gaattttatc
     1141 caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat ggttttacta
     1201 ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga tccaactgcc
     1261 caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat cgatggattg
     1321 aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc aaacgtccct
     1381 tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt aagccgctta
     1441 tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact agatgtaacg
     1501 attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg
     1561 caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg
     1621 gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac aaactggata
     1681 ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga atatgtttta
     1741 gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat tttgccgaat
     1801 atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat tttcttcgaa
     1861 gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt aggaacgatg
     1921 ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat tccggcagta
     1981 actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg tgatgccttt
     2041 gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa gacttagaaa
     2101 tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat tttcatttga
     2161 ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca gtgacgacca
     2221 gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa atcctcttta
     2281 aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa
     2341 tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc attgggaagc
     2401 aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga caaaaagctg
     2461 cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc aacagttatc
     2521 cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt
     2581 atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact attcaagcac
     2641 agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt atctttatca
     2701 ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa
     2761 gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat ccctatactt
     2821 ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg tatgctattc
     2881 ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat ccaagaaatg
     2941 aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta tcaaaaacac
     3001 acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg ccagaagaaa
     3061 tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt caaggaggaa
     3121 ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt caatgtagga
     3181 aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga aggagaaaca
     3241 ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac gatcattcgc
     3301 ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac gaagatcaaa
     3361 gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca agatccatac
     3421 gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat tgatgttaat
     3481 ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt gagaactgtt
     3541 ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg gcaacgccag
     3601 cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg tgatgaacca
     3661 atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca agatctccaa
     3721 aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt taaacatatt
     3781 agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac aagtgatgaa
     3841 atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat tcctctacca
     3901 gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc agatgacgga
     3961 caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac tgagcaggaa
     4021 gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt tgctaactag
     4081 gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa gaacaaaaac
     4141 agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg ctgaaaaagc
     4201 ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc gctacattaa
     4261 aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat gcacagctta
     4321 atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca aagcttaaaa
     4381 cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg atctatctgg
     4441 aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat cagcatcggc
     4501 cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa gaattttatg
     4561 tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa tggatcggga
     4621 tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta actttaccaa
     4681 aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc
     4741 gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag aaacaaaaga
     4801 gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat cttgaacaat
     4861 tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt ccaaaagaag
     4921 aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa ttgcgttata
     4981 agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca gaagaaagca
     5041 attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt atcaagggat
     5101 aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa aagtcttaat
     5161 gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg acaaaccaat
     5221 aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt ttttattcag
     5281 aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa gagttgtttt
     5341 tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat tcatggtgcc
     5401 ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt tcaccgatct
     5461 catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt ttataatcga
     5521 tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct ttacctaaaa
     5581 cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat gctttcaaga
     5641 tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca gtgactaaat
     5701 caagcgagat agccacttct ccgctagata aatggatctt gttactatga cgttctagtg
     5761 tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct ccaataaagg
     5821 ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta ctgaatgtat
     5881 gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt gtttctctta
     5941 agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat gcccatgaat
     6001 ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct tcttctttgt
     6061 tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct ggacgttcct
     6121 gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa tataattttt
     6181 cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta aaagtacttt
     6241 ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta
     6301 tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa agcagataaa
     6361 acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata acgatatata
     6421 cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa tgcccatgga
     6481 ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt gctaatgaaa
     6541 gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt ccagtccctt
     6601 actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa gccgtttttt
     6661 agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc ttttaaagga
     6721 tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa aaaagctaac
     6781 agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc gataaaggag
     6841 gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct attatccaag
     6901 cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa ttgctcgtta
     6961 taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac atggtcaaaa
     7021 aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc
     7081 ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat tcattcgcac
     7141 gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat tagaacaggg
     7201 agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag aattctttac
     7261 agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc
     7321 gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc gcatgagcaa
     7381 atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc agccagaatc
     7441 tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc ttgcagtttc
     7501 acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg tggtgtatgt
     7561 atggatcgat gcattatcca attatattac tgcactagga tatggctccg aagatgaaag
     7621 tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt
     7681 tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt
     7741 atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt
     7801 cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt attatttgct
     7861 tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg tttcccgtgt
     7921 caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg ctatgatcaa
     7981 caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat ttgatagtga
     8041 actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga
     8101 attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca aatacattga
     8161 tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt
     8221 catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc caatcatgac
     8281 agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa tgaatcttga
     8341 aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag gtacaccaat
     8401 cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg
     8461 tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac
     8521 gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt
     8581 catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac tagatgctgg
     8641 agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg atccaagcga
     8701 attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca
     8761 aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac aagtaatcga
     8821 agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag ggggtatgac
     8881 actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt aaattccttc
     8941 ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa taaatcctag
     9001 aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa taggaatcgt
     9061 aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga ttcatctggt
     9121 actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta ttacatctat
     9181 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
     9241 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
     9301 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
     9361 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
     9421 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
     9481 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
     9541 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
     9601 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
     9661 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
     9721 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
     9781 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
     9841 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
     9901 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
     9961 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
    10021 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
    10081 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
    10141 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
    10201 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
    10261 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
    10321 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
    10381 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
    10441 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
    10501 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
    10561 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
    10621 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
    10681 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
    10741 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
    10801 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
    10861 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
    10921 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
    10981 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
    11041 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
    11101 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
    11161 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt gtctttattt
    11221 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
    11281 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
    11341 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
    11401 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
    11461 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
    11521 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
    11581 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
    11641 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
    11701 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
    11761 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
    11821 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
    11881 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
    11941 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
    12001 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
    12061 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
    12121 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
    12181 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
    12241 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
    12301 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
    12361 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
    12421 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
    12481 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
    12541 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
    12601 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
    12661 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
    12721 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
    12781 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
    12841 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
    12901 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
    12961 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
    13021 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
    13081 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
    13141 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
    13201 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
    13261 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
    13321 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
    13381 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
    13441 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
    13501 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
    13561 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
    13621 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
    13681 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
    13741 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
    13801 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
    13861 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
    13921 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
    13981 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
    14041 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
    14101 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
    14161 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
    14221 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
    14281 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
    14341 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
    14401 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
    14461 tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct tggtacttgc
    14521 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
    14581 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
    14641 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
    14701 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
    14761 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
    14821 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
    14881 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
    14941 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
    15001 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
    15061 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
    15121 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
    15181 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
    15241 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
    15301 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
    15361 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
    15421 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
    15481 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
    15541 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
    15601 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
    15661 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
    15721 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
    15781 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
    15841 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
    15901 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
    15961 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
    16021 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
    16081 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
    16141 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
    16201 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
    16261 actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa
    16321 atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat
    16381 aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca gaggatcacg
    16441 gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg ggatcagcaa
    16501 attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg agacccgctg
    16561 gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga tgtctatgag
    16621 attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact ggaagccggc
    16681 ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc attttgatgg
    16741 ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa aagctaaaca
    16801 aactgataat atgcgatata atacgaacgt attggataat ataaacaata atattagttt
    16861 ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa
    16921 taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg cctcttggac
    16981 gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga tgtagcagct
    17041 cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga ttgctattat
    17101 gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg agaccccatt
    17161 tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt ttggtccttt
    17221 ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct tctcgaaatc
    17281 tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct acggattctt
    17341 tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta gtgtcacgct
    17401 gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg tgccattgtt
    17461 cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac cgattcaatc
    17521 aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa gaagtttagc
    17581 gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct tgtttttatt
    17641 gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga agcagctcat
    17701 catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg tccgcatttt
    17761 tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc attgctaaga
    17821 aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct tcatctaaga
    17881 tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg cgttgtccat
    17941 ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc aaacgttcta
    18001 caaattcata catataagag gcttttaggg catcattgat ttctgcgtca cttgcttccg
    18061 gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca
    18121 ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca tggtcatcga
    18181 ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca agtgtggatt
    18241 ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca gtaaaattga
    18301 aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca aaagagactt
    18361 ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc ttttcttctg
    18421 gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg
    18481 tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc gtaaagctgg
    18541 aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg ccaactaaac
    18601 caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc aaggaagaag
    18661 aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca aattgctgtt
    18721 gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt cgttcggtca
    18781 caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt tgtgaagcac
    18841 gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg accaatgtca
    18901 gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc actccagaaa
    18961 ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca tttgtaaatc
    19021 gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat cgattcagat
    19081 gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga ttgcttaaca
    19141 cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat agaataagta
    19201 aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg atactttgac
    19261 caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt atcacagcta
    19321 gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg
    19381 ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt cctgatattc
    19441 tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac cttgatccat
    19501 cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa taatcactgt
    19561 actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat aatccaacgc
    19621 acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt
    19681 cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt ttgtatctaa
    19741 accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag cttcccaaca
    19801 ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag tcccggtaaa
    19861 tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca gctgccaaga
    19921 acgaacatcc attccgtcca ttataactgt accggaactg acatcataga aacgcggaat
    19981 caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa ggactgtatt
    20041 tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag tataacggaa
    20101 gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag tactgacctc
    20161 agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc ctgaagcttc
    20221 ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa gcatttgatt
    20281 catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat ttactttgat
    20341 cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg tagcaggtga
    20401 taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat ttgtgctttt
    20461 atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac gaacaccgct
    20521 aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt tttggtagag
    20581 aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca ataatgctaa
    20641 gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc cgatacttaa
    20701 aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa gctgattgat
    20761 gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga
    20821 gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc cttgtgaagc
    20881 tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag acatagcgac
    20941 catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc cattatcgat
    21001 caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa
    21061 cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact gaatcatatg
    21121 attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac attgtagctt
    21181 aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt attatgaaga
    21241 agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa aacatgccag
    21301 tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa ttctgcataa
    21361 aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt taatttgttc
    21421 ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga
    21481 ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc
    21541 ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga
    21601 tggaacgaag acaacctccc gacatcaagt caaaagccac ttgagatttt gatctgaaaa
    21661 taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg
    21721 aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta
    21781 ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca
    21841 catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt
    21901 ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt
    21961 cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa
    22021 gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc
    22081 aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt
    22141 attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa
    22201 taaaaattat aagggataag taggctgctg tctataataa gatatatttt tcaattcttt
    22261 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
    22321 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
    22381 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
    22441 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
    22501 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
    22561 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
    22621 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
    22681 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
    22741 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
    22801 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
    22861 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
    22921 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
    22981 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
    23041 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
    23101 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
    23161 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
    23221 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
    23281 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
    23341 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
    23401 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
    23461 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
    23521 aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg
    23581 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
    23641 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
    23701 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
    23761 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
    23821 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
    23881 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
    23941 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
    24001 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
    24061 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
    24121 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
    24181 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
    24241 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
    24301 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
    24361 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
    24421 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
    24481 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
    24541 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
    24601 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
    24661 tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt
    24721 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
    24781 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
    24841 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
    24901 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
    24961 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
    25021 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
    25081 aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta
    25141 tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg
    25201 aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat
    25261 tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct
    25321 ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt
    25381 caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa
    25441 tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt
    25501 gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa
    25561 tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc
    25621 gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac
    25681 tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa
    25741 ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg
    25801 ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat
    25861 gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag
    25921 gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa
    25981 cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat
    26041 aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc
    26101 agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt
    26161 gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac
    26221 tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc
    26281 ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc
    26341 tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat
    26401 attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta
    26461 tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct
    26521 ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg
    26581 aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt
    26641 gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc
    26701 atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc
    26761 tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc
    26821 acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca
    26881 aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt
    26941 ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa
    27001 agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta
    27061 acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa
    27121 ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg
    27181 gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg attgccacag
    27241 gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga
    27301 ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag
    27361 gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg
    27421 cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata
    27481 ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata
    27541 tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag
    27601 aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga
    27661 tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa
    27721 gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg
    27781 aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca
    27841 gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat
    27901 tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc
    27961 gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt
    28021 gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat
    28081 ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta
    28141 cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata
    28201 gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa
    28261 aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc
    28321 accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa
    28381 acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg
    28441 gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt
    28501 tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca
    28561 aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct
    28621 gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt
    28681 attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa
    28741 gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca
    28801 ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac
    28861 gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat
    28921 atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact
    28981 ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact
    29041 caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt
    29101 atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct
    29161 cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga
    29221 gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta
    29281 gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt
    29341 gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc
    29401 atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt
    29461 cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa
    29521 gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat
    29581 tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga
    29641 cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag
    29701 cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat
    29761 agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt
    29821 tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa
    29881 cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact
    29941 atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac
    30001 atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag
    30061 agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg tctcttatgt
    30121 attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt
    30181 aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa
    30241 atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac
    30301 aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa
    30361 ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg
    30421 gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt
    30481 ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt
    30541 tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg
    30601 ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg
    30661 aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac
    30721 aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac
    30781 aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca
    30841 caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca
    30901 tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag
    30961 cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg
    31021 atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga
    31081 tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt
    31141 gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc
    31201 tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag
    31261 cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg
    31321 ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa
    31381 aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc gtcgtttagg
    31441 aggaacagca agtgaaggtc aaaaaagcag tcattcctgc cgctgggcta ggaacacgtt
    31501 ttttgccggc aacaaaagcc atggcaaaag aaatgcttcc aatcgtggat aaaccaacga
    31561 tccagtttat tgtggaagaa gcattggatt cgggaatcga agacatcttg atcgtaacgg
    31621 gaaaagaaaa aagacccatt gaagatcact ttgatgcaaa tatcgaattg gaaaacaatt
    31681 tgcgggaaaa ggaaaaaaca gaattactcg ctttagttga agaaacgact caagtcaatc
    31741 ttcacttcat ccgtcaatct catccaagag gactggggga tgccgtgctt caagcaaaag
    31801 catttatagg aaatgaaccg tttgtcgtca tgctggggga tgatttgatg aaagatgata
    31861 tcccattgac tcaacaattg atgatggatt atgaagaaac acaagcttcc gctgttgccg
    31921 tcatgcgtgt accagaaaat gaaacatcta aatacggaat tattgatcct aaaacagata
    31981 gcgaaaaggg tcgttgcaga gtaaaagggt ttgtagaaaa acctgaaatg ggaaaagcac
    32041 caagtaattt agccattatc ggacgttatc tactgactcc taaaatattt gagatattag
    32101 aaactcaaga accaggagca ggaaacgaga tacagctaac ggatgcactt cagactctta
    32161 accagacaga ggcggtctat gcgagagagt ttaaaggaaa aagatacgac gtaggcgata
    32221 agttaggata tatgaagaca aatattgaat atggacttca acattcggaa attgggactt
    32281 cattaagtgc atatattatc cagttgagta aaaagctaag aacagaaagt taactggaaa
    32341 taagtggcct gctgagaatg aaaggctatt tgttattaaa gcggcagctt gtgcgtcatt
    32401 tgcgcaactg tcgttttagt tttttgactc tagatcagtt gagtcgtgga aaacgaaaga
    32461 tagaaaaaga ctttcgtgaa tagattcaga aaatattatg ctaatggttt aatttttttt
    32521 taagttgtat aaaagaaaat gattacataa gagaaatgaa agccaagcca gaagattcat
    32581 agtagaataa aaaggcgaat tttttaatta taaaaaaagg tgtgtgttca ctatgggaaa
    32641 attagtcgct gttgcacagt cgctgcttga tatactcaag tggctgatgg aaggaagcaa
    32701 ttggatattt gtgttgccac tattgcttat tagtctattt atcatgtttc gcatggtcag
    32761 aaaaaaagaa acgtttctga tttttatctg gtatgcagca tccgttgggg ttgctacctt
    32821 gatggttgaa ttggcgttag gtagttggga agcttgcgta tttgctttat tttttgctgt
    32881 actgtatcta atcatttata tcccatatat gaaaagaaag tctcggatag gcagaaaagg
    32941 tagacataca actacttttt ctagtacaga ttctctcgaa caaagtagaa gaacgagaag
    33001 aagacggtaa tatttagtag gtatacgcaa aaaaagataa aataatggat aaactattga
    33061 gatgagagat ttatttaatt ggtggaaaaa agcttttttc acttatataa aagaatcttt
    33121 tttaaaggaa tgcttgttgt atcgggataa gccattcctt tttttatgtt atttatatat
    33181 atataaatca taccaatgtt tttttgtatt ttcttgcttg cacttggata agagattact
    33241 tacaataaat tcagatagaa acagctgaat aaaaaaaggc tccctgaccc ttagttggcg
    33301 ctaagaatca gggaatgacc agtcacacac atacaccctg accgatcatt ttgccatagt
    33361 caatgcagtg catcgaccta atttattgta cctaatctag ggtgaaaatt ctacccctat
    33421 gtagatattt tccatggtct gcacgttgtt ttatttgcta tgtcaaggta ttgggcgtat
    33481 tcgttcaatg ccttttttgt gtttgaaaca gcaaagacca catgtgttac tgtctaacta
    33541 ctgttttttt atgtacttcc ttagattagg cagctgtttc tattcatgtc aagtatagct
    33601 aggcgggaga gataaatgag tttatgttat tttgactatt atcaccaatt ggaagagaaa
    33661 ttaaggatag aagagccgct gatgcttcaa gctggcactt ggatcgcgaa acaaattaat
    33721 caaggtggta gattgcagat atatgctagc cgcacactaa gtggtgtagc atttgaattt
    33781 tgggatcaaa ccccggatct tgtaccaagt atattgattg aaaatccagc cggaggtgtc
    33841 tatgaaacgc tagaaggtgc aggacaggca attattgatc agctttctgt caaatcagaa
    33901 gacatttttc ttcttctttc gaatgaagga agaaacccgg caatcatcga actagctcag
    33961 tggatcaaag aaaatggtca tccattaatc attgttactg gatttgattt gtcacgaagt
    34021 atcaaatcgc aacatagcag tggtttgcga ttatttgaat ttgctgattt ggttttggac
    34081 aaccacgcca atttacatga tgcagtcttg actttgccaa atctagatcc agcgatctgc
    34141 ggaacagcct ctcttgcaac gatctttttg ctgcagcaag ttttgtattt tacagcaaaa
    34201 cagctagtcc aggacacaag tggaaaatag ttatccacat gttgacaaag ttccttaaaa
    34261 aaataatgtt ccacataaaa atggagcgtt tgaaaaaaag aaaacgatta ttaatgagga
    34321 ttaacaacga ctactttttt tgttttagtt attttaagat ggatcatctt tgattttaag
    34381 cttgttttgt ttaaagtttg ttatttttat aagttgtttc acagttatga gaaatgagtt
    34441 ataagaatat gtagtgtcag aaaaataaaa agtagtggaa aaagagacaa gaaagaatcc
    34501 atatggattc tttcttgtct cttaatacac gttgatgaaa gagtcttccc caaaactctt
    34561 tacaaagtaa ttatagtaca tttgttatca ttattgcaat gaagtttacc tgatataaca
    34621 aaattgtcca acaggatacc tttttctaat agaaataaaa tacacaaaaa ttgaaggata
    34681 gataatggat atttataagt gtttattatt ttttttgcaa tgaaattcaa tttttcaatg
    34741 attgtaaaat gaaaaaaaca ggacgagatt ttctctaatc taaaaataaa aaaagaattt
    34801 taaggaaaaa cagagaaata aaatacttaa tataaatgga aagagaaaaa aataatcata
    34861 cataggagaa aaaaaacaaa atagaaaaaa gtgattttct aataaattat tcatgtggaa
    34921 atgtcatttt tactgtataa ttcatttgat tatccatttt ttattcatca aaattactga
    34981 aaaaagaaag gaagaaataa atttgaagaa aacaaattct tcaggaaaac cgaaacgcga
    35041 cttcactaaa ttatcgacac cccatacgta tgtcattatt tttggtgtag tcatctttgc
    35101 atggatactg acttttgtcg ttccagcagg aaagttcagc acgcaagata ttgaatacaa
    35161 agatgcaaat ggcgaaacaa gtactcgtac tgttttacgt caagattcct tccgttatgc
    35221 ttacgagctg gataagtcgt atgtgtttga tcaattagaa gaactgcaag atcatccagc
    35281 agaacgtgaa aaactggatg ttccggaaaa aggattggaa aaagtcattg ctgacggaga
    35341 aaaaaatctt acgcaggaaa aactagatga aatctccttg acagatgatg tgctttacga
    35401 tcagtacggc gaaaatatct atgatacttc taaaaaattg cataaaacag ccaaaatatg
    35461 gggaacagat gattttggcg gctttggttt cttaaatttt gtttttgaag gactagtatc
    35521 tggagataag tatggatctg ctgtagggat cgctgcattg atccttgttg ttggaggtgc
    35581 ttttggtatt attatgcgta ccggagcgat cgacgcaggt atctatgcgt ttatcagtaa
    35641 aactaaggga ctggagcgtt tagctttacc gctattattc tttgcttttt cattcggtgg
    35701 cgctacattc gggatggcag aagaggtaat tccattctca atggtgatgg ttccatttgt
    35761 cattgcgtta ggatatgact cgatcgtagc agtaaccgtg acttatgtcg catctcaagt
    35821 cggaaatgcg acctcttgga tgagcccgtt tagcgtagcg gtggctcaag ggattgctgg
    35881 aatccccgtg ttgtctggtg cgacattccg attgattatg tgggtagttg ttactgcatt
    35941 agccgctggt tatatgatga tatatgcaga aaaaatccgt aaaaaaccag aaagttcgtt
    36001 gacttacaaa tcagatgatt atttccgttc tcatatcaaa aagacatctg atgaaaataa
    36061 aaaattcatg cttgggcata aattgatctt attagagatg ttagttgtcc ttgtctggat
    36121 cgtttggggc gtgactcaaa aaggctatta cattccagaa atcgcttctc aattctttgt
    36181 gatgggatta gttgctggaa tcattgctgt tcttttcaaa ttagatggaa tgaaagtcaa
    36241 tgacatcgct tcttctttcc aatctggtgc agctgattta gctggtacag caatcgtagt
    36301 aggtatggcc aaagggattc ttcttgtctt gggaggatct gatgcatctg ttccttcagc
    36361 tttgaatacg attttgcatg ggatcgggac ggcgttgacc ggtgttccag ctgtcattgg
    36421 tgcttgggcg atgtacatct tccaaagttt gttcaacttg gttgttacat caaattcagg
    36481 acaagcagct ttgactatgc caatcatggc tccactggca gatttagtag gtgtatccag
    36541 acaagtagca gttcttgctt atcagcttgg ttcaggtttt atggacgctt ttacacccgt
    36601 atcagccagc ttgattggtg tgctaggtgt tgcacggatc gaatgggcaa aatgggcaag
    36661 attccaaatc aagatgcaag gattcctgtt tgttttagga actattttca tcatgattgc
    36721 cattgcaata ggtctgcagt agtactggag acctgtaaat aaaaaagtaa tccaaacagt
    36781 agataaggac tccaagaacc gtcattttac ggtaaaatca aaggagtaaa gatcctatcg
    36841 tttggattat tttttaagat cctatcatca gttatactta aagaaagagg aaaaatgtat
    36901 gaaaatcatt cgacaaattg aagtgtttgc accagattac ttaggaaaaa tggatgtact
    36961 gattgccggt gggaagattt tggctgtaga agaacagcta gaaggcggct acgaaggagt
    37021 ggaagtagaa gaactttcag gtgagggaaa agtactaacc ccgggtttca ttgactgtca
    37081 tttccatatt ttaggtggag gaggagaagg cggttatcaa aatcgaacgc cagaagtcac
    37141 attgagccaa ttgactacag ctggtgtaac gacagttgtc ggttgtttgg gaacagacgg
    37201 agaaggtaga gatatgactg cgttgatcag caaagcgaaa ggtctggaag cagaaggaat
    37261 ctccacttat gtatacgaag gttcgtaccg attgccggta aaaacagtaa cagattctat
    37321 catcaaagac tttttgacaa ttgataaaat tatcggaatc ggagaaatcg cagtatccga
    37381 tcaccgttct tctcagccaa gttttgaaga gttcgctcgt gcagtagcag atgctcgtgt
    37441 aggaggcatg ttatccggaa aagctgggat catcaatgtc cacttaggcg gaggaaaaag
    37501 aaagctagag ctattgacac gaatcgtaga agaaacagaa attccgatca cacaattttt
    37561 gcctactcat gcaaatcgca caccagaatt atttgaagct tgcgtggcat ttgccaaacg
    37621 tggtggcacg attgacttca ctgctagcga agatcctgat ttttgggaga agacagatgg
    37681 tgaagttcgc ttcagcaaag cactgaaacg attgatcgag gaagatgttt ctttagataa
    37741 tttcacgatg acctcagatg gacaaggaag cttgccttat tttgatgaaa acaatcattt
    37801 cctagggtta ggagtaggaa gtgccaaggc attgctagta gggatcaaag aagctgtgca
    37861 aaaagaaagt atccctttgg aaatcgcctt acgagctata accagtaatc cagcacgtat
    37921 attgaaattg gataaaaaag gaaaaatcga aattggtgct gatgcagatc tgtgtatctt
    37981 ggataaagaa acgcttgata ttgatacagt tatcgctaaa ggagaaataa tggtccaaga
    38041 aaaagaagtc aaagtatggg ggactttcga aaaaagtttt taatcaacag ctctaactat
    38101 cttttttaga aaacgagcgt tctgtttgtc tgtatctgtt gttgatgtta ccctgaaggt
    38161 agatcaataa acaaactaag aggaggaagc acaatggcag atttaaaagg acgttctaaa
    38221 gatatgaaag ataaagtagt cggtgaagga aaagaagcat acggcaaagc aacagacgac
    38281 aagagcaaag aaacagaagg gaaagcacaa tcactaggtg ctgacatcaa agaaaaagct
    38341 cgaaatttag gcgacgaagt caaagacaag ttttctcgaa aagacgaaaa aaaataaaaa
    38401 ccacttatta ctaattgaat caagcaaata aaaaatccga accatatctg cgagacagat
    38461 atggttcgga ttttttattt gatcattatc ctgtttttta aacttcgatt ttttgaaatt
    38521 ttttattttt tagttcaaag acttcatcac agctttttgc tacttcaatc tcatgagtca
    38581 cgatgataat acagcgattt tgttcatggg cgatttcttg gaaaagtttg acgatatctt
    38641 gtgaagttgt ttcatctaga tttccagtag gctcatccgc tacgattagt tgatgttcac
    38701 agcagatagc acgaacgata gcgactcgct gctgctgacc tcccgaaagt ttggtcacct
    38761 gtttttttgc taatatttca tcaattccta cacgagccag tcgatctaaa gcaacttgtc
    38821 gataattctc ttgcttggtc ttagtgattt ccattgctgc tatgacgttg tctagagccg
    38881 ataaataagg aagcaagttg taagcttgga aaataatcga gacatcattt ttacgataac
    38941 ttcttaaccc gattttgttc agtgaacgat cttgatagta tacctttcct tcttttggtg
    39001 catctagtcc agaaataagc gaaagaaaag tcgtttttcc agaaccactt gctcctaaaa
    39061 tagcgtacat ttttccagct tcgaaattca gttctacatc tttataaagc gcatcttgcg
    39121 gatcttgata ccaaaatccc aattgttctg tccgtaacat caaattcctc ctttagttta
    39181 ataggatttt tcttgggttt aatcttaata tccctataga agctaaaaag acagaaaaca
    39241 gactgattcc aaatgctact ccggctaata gtcccaattg tgcaggtttg acattgattt
    39301 ccaattcgct gacttgatca gaaacagcaa atggattatt cgtggttcct tgttgaccat
    39361 cgcgttgacc gccttgttct tgtctgttac ctggcatttg tccaccgcca ggagcttgct
    39421 ggttttgtgt cgtggtttct gtctgttggt caagtagctg ctgtcccacg gcattcgcga
    39481 cgacgtttcc gctaagcgaa gcaactccta atgcaagaat catacaaata gccacttcgg
    39541 taaacaattg gagaatgatt ttacttctgg attctcctaa agatagcaag acgcctaact
    39601 catgccgacg ctcccgaatg gtaatcataa tgatcagagt aagtatgata ataccggcta
    39661 cagccactag taaaacaaca tttttagaaa aacttgccac gttgtttaaa ggttccaaca
    39721 ttgactgata catggaatca tttgattgta agctaaatgt atctgtgtcg atcagcttct
    39781 cagcttcttt gagaaactca tccattttat ctggatcaga tagagtgtaa atagcagaat
    39841 ctatcgtgtt atcgtcagat gtcccattta gttcatttgc aaaagtgtaa gaagtgtaaa
    39901 gtgtattcga cggattcatg aagttgaatt tcatacccat actgccgctt gtttcgctgg
    39961 attcataaat tcctttgatc gtcatttcat aagtggtatc ttcatcttca gtactcgtga
    40021 ttgtgaaagt atctccgact gccaggtcat ttgtttctgc tagagtagaa tcaatgacta
    40081 cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc atccgtgatt tttgctgttc
    40141 catcagaaaa attactattt tgtgcagttg ctgacacacc actgatttga aagtctgctt
    40201 gagtcatttg cggattcccg cccattcctc cagcaggttc accgcctgca tcagtagttt
    40261 ctgaactgtc ttcacttgaa tcagaactag tgatggcagt tattccatct tttgccatag
    40321 ctgaggcgga actttcaaag gaataactct ttacattatc taattcagct attttttccg
    40381 cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg atctggacgt tcttcagtgg
    40441 tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga agcttctcga ttggcactta
    40501 aagtcactgt tgctccaact tctttttgtg cttgattcgc tgcttcttct gcagcgcttc
    40561 gaatcgtgag gccagctagc acaaaaataa gaatggctgt gaacacggca aacaataaca
    40621 atgaacgtcc ccattttact tttgtacttt tccatgcacg tttcataaaa ttcatatttt
    40681 gcatccctcc tagtagatgc atttgataat agacaaccta tcttaaagct agcttaaatc
    40741 ttatgctgtt tgcacaccat caacatgagg ttctgtatat ataaaaagtc actatagtta
    40801 ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa ccgacgaaga agaaatgaaa
    40861 gaggaatagc aaaagaaatc ctagatgcta cagagggatt tttacgtgca tagttgttct
    40921 aacaattgct gatactagcg atttagttta tttgttgtat aataatgggg aacagctgaa
    40981 tacttatata agtattctgg ctatagcgtt tcgcaaaaga tccattttgt taatgaagta
    41041 tatccaaaat aaaatgccca tccaactgtc agaacgcgga tgtgctggta attttcctgc
    41101 tgattctcca taagttgaga aatagtgatt ttgcatatgc ttcattggaa tggctcatat
    41161 atttagataa agaagaaaat cgacatcttt aaacttggat gtcataagaa gaggtgaagt
    41221 ttgtgagtac cattaaaatt atcccattag gcggcgttcg tgaaaatgga aaaaacatgt
    41281 atgtcgcaga agtgggcgag gatattttta ttttagattg tgggctaaaa tatcctgaaa
    41341 atgagttgtt ggggatcgac gtcgtaatcc ctgattttac gtatttagaa gaaaacattg
    41401 atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga tgcgatcggg gcacttcctt
    41461 acttgttgtc aaaaatttct gtaccagtat tcgggacaga gttgacgatc gagttagcaa
    41521 aattgaatgt cagtcgtaat gaagcagcca aaggatttaa ggatttccat gtggtagatg
    41581 agcatacaga aatagacttt ggtgaaacag tagtgagctt tttccgtacc actcatacga
    41641 taccagattc agtggggatc aatctgaaaa caaaagaagg tagtatcgtt tatacgggag
    41701 attttaaatt tgaccaaacg gctatcccaa tgtatcaaac agattatgca cgattggcgg
    41761 aaatcggcaa agaaggagtc ttagccttac taagtgaatc ggcaaatgca gaaaatcctc
    41821 agcctgtagc ttctgaagcc caaatagcag atgaagtatt tgatacgatc aaatattggg
    41881 aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca acgagtgcaa caagtattgg
    41941 atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg tcaagatttc gaacgaatta
    42001 ttcgcacagc gatgcgttta gaaaaacttc aattaccaag tgaagatctg cttgttacgt
    42061 tgaaagacat gaaaaactat gatccagaag aactgatcat tttagaaact ggccgaatgg
    42121 gtgaacccat caagtctctg caaaaaatgg caaacggtac acaccgtaat gtcaagattg
    42181 aagaaggaga tttggtttat atcactacta cgccaagcat cgcgatggaa accatcgtgg
    42241 cgaaaacgga agatatcatt tatcgagctg gtggaacagt gaagttgatc tctgagaata
    42301 tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt aatgatcaat ttgatgaaac
    42361 caacatattt tgtgccagtt caaggagagt atcgtgaatt agctgctcat gcagatttgg
    42421 ctcatgcagt agggatgcca tacaaaaata tctatattac tggacgcgga gacgtgctgg
    42481 aatataagaa caagcgtatg agtgttgcag gaactacaag tgcagaaaat gtaatgattg
    42541 atggattagg cataggtgat atcggaaaca ttgttttacg tgatcgaaaa atcttgtcag
    42601 aagacgggat tttcgtagcg gttgtcacta tcagtcggcg agaaaagaaa atcatttcaa
    42661 aacctcaaat tacttcacgg ggattcgttt acgtaaaagc tagccgtgat ctgatgaaag
    42721 aaagcagcaa tatgattgaa acaatcgttg agaaacattt agaaagcaat gattttgagt
    42781 ggagcaaatt aaagcaagac atccgagatc aattaggccg ttacttattt gaacaaacaa
    42841 aacgccgccc agtgatcttg ccagtcatta tggaagctac tcaacgtcga aataaaaaat
    42901 aaacaaaaag agtaagatga acctctctat agagtgctcg tcttgctctt tttttgcgaa
    42961 aaacaaataa tttttgaata cagcatccct cgctttataa taaacgatag aataaaggga
    43021 gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt agaagaacaa aagatcagct
    43081 atcaaccact tgattttagt catgtggtag aagaatcttt cacagaagaa ctacaaaaaa
    43141 gagggattga tccagctttg atatgtaaaa ctctcgtagt gaaaggaaac aaaactgggg
    43201 tgatcatcgc attgattcct ttgaacgatc acttagatta caaaaaaacg agacaagtga
    43261 cgaaagatcg taaagttggt tttcctggaa tggattttgt attaatgcat acaggatatc
    43321 cccacggagc aaacacccct attggaataa aactcgcaca tcctgattat tgttttttag
    43381 cagacagttc tttaaaagaa aaagaagaag taattgtgtc aagcggtgaa atcgggagaa
    43441 gccttcggat aaaagtagcc gatttagaaa agatcgtcca tcctatttac gctgatttaa
    43501 ttagttaagg gacgagaaga gaagctaagc agagcagaga cctcaaactt gagattatat
    43561 ctgcttggta cttggtgcat cctgtattat ctgttacaat tgctttgtac atactgtact
    43621 atcaatccaa tgaaaatata tttagcagct atattttttc attgatgtgc aagtgatgaa
    43681 atcgttttta ataaaaaaga tacagagaaa acgcgaggtg taaaatatga aaattacgta
    43741 tcatggacat tcctgtttaa caattgaaat ggaaaatggc caacgcgtga tgattgaccc
    43801 gtttattaca ggaaatccat tgacagactt aactgcagaa gaagtaaaga cagattggtt
    43861 gctgattact catggtcata gtgaccacat aggagatatg attcctattg caaaagaaaa
    43921 tcaagccaca attatcagta ttgttgagat tgctgattat gcgaacagca gaggagtaga
    43981 ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca ttcggcgtgg taaaattcgt
    44041 acatgcacag catagttcaa gctatgaagt agacggtatc gttcagtata tgggcgagcc
    44101 tagcggaatt attattcaag cagaaggaaa aacgatttat catgcagggg atactgctta
    44161 tttcagtgat ctaggattgc ttgcagaaga attcgatatt gatgtcgctt tcttgccgat
    44221 tggcgataac tatacgatgg ggccagaaga tgcagcaaag gctgcgcaaa caattcgtgc
    44281 caagaaagta gttccaatcc attacaatac tttccctgtc attgaacaag atcccgaatc
    44341 ttttgtagag ttactggaag atagtgaagg aaaaataatg agcgtctcgg aaacgctgga
    44401 agtgtaggca gaaaatggcg acaaaacatg atttgatttt agaatatatt gaaagtttac
    44461 ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga attgaatgtc agtgaaggaa
    44521 cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact agtttcaaca attcaacgag
    44581 tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat tgaacgtttg acatttggcg
    44641 aggtcgtcag gatcattgaa ggggacgttt taggcggggc ggcaggatta gacaaggtct
    44701 tgaataaatt cgtcatcggc gcgatgacta aacatgccat ggaacgctat attacgccag
    44761 gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa attagcattg gaagatggcg
    44821 ccgcagtatt gatcacagga ggattcaata cgactgctga gattgcaaag ctagctgatg
    44881 atcttgaaat gccgatctta cgcacctctt atgatacatt tacagtcgca accatgatca
    44941 accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat gcttgtcagt gatatctata
    45001 tgccagctga aaagactcat tatttgcagc agacagatac gatttctgat tatcagcgtt
    45061 tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa ccgccatcat cgtttgatgg
    45121 ggattgttac ggcaaaagat gttttaggga aatcgccaaa ccagatcatc gatcgggtga
    45181 tgacgaaaga accaatcagt gtaaagaaaa caatgagtat tgcctctgtt agccatcaaa
    45241 tgatctggga tgggctagaa gtcatgccgg tcgtttccga tgacttgaca ttggaaggct
    45301 tgattacccg gcaagatgta atgaaagcga tgcagcttgt ccagcgacaa ccgcagattg
    45361 cagatacgat ttcggatcag atatctggaa atatccatac gattgatact gaccgtgaag
    45421 gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc accacaaatg gtcaatggcg
    45481 ttggaaccat ttcttttggc gtacttagcg aaatcatttc tgatgtcgca caaaaaacaa
    45541 tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt gaacctacat tatttgcggc
    45601 tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt attggaaatc ggcagacgtt
    45661 ccgctaaact ggatatcgaa gtgtttattg aaaacaccat cgtttccaaa gcaatcgttg
    45721 tttgtcaagt aatggaacgt tcttagaaag caggagtgaa atgacagtac agcaagaaat
    45781 agtagataca ataaaaaaat acgataaaat cattatccac aggcatttgc gccttgatcc
    45841 tgatgcatta ggatcacaag taggattagc cgaactatta cgggaaagtt tcccagaaaa
    45901 agaaatacgt caagtaggtg aaacgattga agggcttcgc tttttagcag aaatgcaaga
    45961 agttgaagat gtcttctacc aaggagcgct agtcatcgtg actgatacag ctaattcacc
    46021 cagaatcagt gatcaaagat atcagttggc agaaaaactg atcaaaatcg accatcatcc
    46081 caatgatgac ccttatggag atcttgtgtg ggtaaataca aaagcaagta gctgtagtga
    46141 gatgattgca gaatttgctt tgatgtttcc tgaatcttta aagatgaacg ttaaggctgc
    46201 acggttactt tatgcaggaa tcgtcggaga tacagggcgc tttttgtatc cctccacgac
    46261 gtcccgtaca ttagagattg cttcgatatt gagaaattat ccatttgatg caagtgcttt
    46321 gaacagagag attgaacaaa tgccgatgaa agtcgctaaa ctttccggtt atctttatca
    46381 aaatatccag gtggatgaaa atggtgcagg aagaataatc cttccgcaag aaattcttga
    46441 taaatatgga atcgatgatt cagagacagc ttcgatcgtt tcacttcctg gagtgattga
    46501 cgaagtgctg gcttggggga tttttgttca gcagccttca gggtattacc gcgtacgttt
    46561 acgttctaag gggccgatca tcaatgaatt ggctaaacgc catcatggag gaggccatcc
    46621 attagcaagt ggtgcgaatg caaaagattt agcagaagta gaggaaatct ataaagagat
    46681 ccaagatata tgccaatcgt ataagagcag aaaaacagaa tgatacttcg ttcgaaaacg
    46741 tgccttttgt agtaaaaatc acaaatgtat cttttcaaga tcttcttttt ccgttacaat
    46801 agtgctcact aaagatttta aaggagaaag aagatgacat taccaaattg cccagaatgt
    46861 ggctcagagt acacatatga agatcgaggc ttgttgattt gcccagaatg cgggaacgaa
    46921 tggaacaaag aagaacaaga aactgtcata gaagacggat tagtggtatt agatgcaaat
    46981 ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta aagacttgaa agtaaaaggt
    47041 gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca tccgtttagt agaaggcgat
    47101 cataatatcg attgcaaggt agaaggattt ggaccgatga aactgaaatc ggaatttgtg
    47161 aaaaagaatt aaagaatatt caaggtcttt tcacattctt ttcctactac taatgataaa
    47221 ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt atggaatgta tggtttttta
    47281 gatccgacat ttcttttggt cattcttgga ttagttattt ccggaattgc gtcagcgtac
    47341 gtcaatagta ctttccgaaa atacgatcaa gtgagaagca aaaacaatgt gactggtaca
    47401 caagcagcac aatatatatt acaaagtcaa cagataaata atgtcggtgt tcaacaaatt
    47461 gccggagatc tcacagataa ctacaattca ggtaataaga tgttgagttt gtctcaagca
    47521 actgcacagt ctacttcagt tgctgcgata ggtgtagcag ctcatgaatg cggacatgcg
    47581 gtacaagatg cttcgggtta tgtcccattg aagattcgtg cagcacttgt tccagcagct
    47641 aactttggtt cgatgatttc gttccccttg attatggtag gtgtattgct tagctggaac
    47701 tctacgttaa tcaatatcgg gattttcgct ttttctttag cattactttt ccaattggtg
    47761 acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc aaattttgag tgaaggcgga
    47821 ctactgacgg aagaagaagt accgatggca agacacgtat tatttgcagc agcactgact
    47881 tatgttgcag cagcgctttc gacattcctg cagctgttgc gcttgatctt attgttcggc
    47941 ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag cgctttgatc tgagctaata
    48001 ccgagagtta gctgattgaa taccatttat ttggaataag aggatgtgac aagaattttg
    48061 tcacatcctc tttgttttgt tctcataacc ttttttcata tacagttcat gaaccgcttt
    48121 tattaacttg ccaaataaca ctgtattgtg cgttgtatcc agatagaaag tctccgtttc
    48181 atctgaaatt ttctcatgct gatctgtaat gatcaaatca gcatgttctg aatatgcggt
    48241 aattttaaca gcatctttgc gaaataactg ctgcaaatgg ctttgaagat gataatttcc
    48301 tgtaaactgt ttggttatct gcaaatggat gagtaactgc tctttcgttt ctgtttgtag
    48361 taagtgttgg atcaattgac taaggtattg actgatttgg tttctttccc cattttttga
    48421 aaaaattgta tcgatttttt ctcttaattt cttttcctgt ataaaagaac tagaatgatt
    48481 ggacgtggct tctggaaagc aaagtgtaga aaaactactg ctggattcac ccaacagttc
    48541 gaaaaaaaca actgccaaca caagaaaata aaaataaatt ggtattttat tttctaaatg
    48601 gttgagagga aactgttgag ttaagtaagt catcaattga ccgacttgtc gacaaataag
    48661 gtttttgctt gtggaaaata aaaaaccaat ctcctgtttt tgtcgaaaag agatttgatt
    48721 tggaaaaaaa caatagaaaa aataattaaa gtaggctctc tctgtttctg tctcttcaca
    48781 agggcgactc actgaaaaag aagtaaagta ttctgaataa tcatgtgtat gctgcaataa
    48841 gtccatcaat tcagtgattt cagaagaagt ttttggaaaa tatgcctgtt gtttgtttcg
    48901 taatatgaat aaagttaaca gcaagtagat atttttttct tgttttgaaa ttttttctat
    48961 ttgctcatct gtactgttca tttttagtgc atttaattca agtgaggaaa atggccaatc
    49021 gttacctcca aatgtatctg aaagaaaaaa gtaaagatag agacggatag ataactcatt
    49081 tccttcaaga gtacagcaat tttttacaga taggatccgt aaatggaatt ttattaagga
    49141 atgattgagc tgttttgtca gtttttcgag ataggagggc gatatataaa gttgttcaag
    49201 taaatatttt tttggtacag cagtttgtgg ataattgatt agtaacatca aatagttaaa
    49261 acgattggat tcttttaggt agcataattt taacttagaa aaaacagcta actttttagt
    49321 ttgatcggtt tctttggttt ggatctctcc tttatgatta gaataaataa attcatcaat
    49381 caataaaaat tgcttgagtg atacatttat ttttcggatc agttctttga ttgtccgttt
    49441 tggaatatgt aagtcattgg ataattcttg taaagaacaa ttattttttt gaatgaccat
    49501 ttgtagaaca agtaactctt tttttcttgc ttcaattagc ataaatacct cctttttatt
    49561 ctgtttttgt atatagttag acaaaaactg tgagtggaaa gtcatttcag tctgtgttat
    49621 tctcacagag tgtcgccttc ttttttatat aacgttattt aatttttttg ttaccttgtt
    49681 tattctattg tttatttcct agggtatttt gtcaagatat tcttgttttt aaggcggaaa
    49741 aattgatttt taatggatat aactaaatat gttttgataa ttatcaataa tatttttaga
    49801 attatttatg ttatttattt atgggtagat gaaaagagac ggaggtaaga atgatgggga
    49861 gagtaacaaa ttatagtaaa gaatatttgc tgttcaaaag tatggtatac gtggaagaat
    49921 atggaatgaa gtcaattact gcaagagagt tagcagagtt ctgcggttgt tctacatacc
    49981 cgatttatac acatttcaaa tcaatcagcg ggttaaaaga gaagatatta gaagaaatca
    50041 ctgtttattt tgaaaactac ttagctgaat gcagttccga tgatgtttta agcattgtct
    50101 acttattgaa agattttttt tctatccatg aaagtattcg tgaaatcatt gcaaaatctg
    50161 atttagacat ggattcgcta tttaagcggc catttttctc atatgtcaaa ggtcacacga
    50221 agatcaaaga agaaaagtcg cttgaattgg tatggcttaa tgcattggga agtttgcgct
    50281 cagatgattc ttctcaagat tttcttgaaa tgatgaacgc tgtttgggat aaaaacgagg
    50341 tgacggatga atacttggaa aatatcttaa aggaacagtc accttaggca gcaacatgaa
    50401 aaagtagatc agccaccttt atttttcgta aaaaagattg taaaatgtct aacgaaacat
    50461 tggaatgaag gaggaagcga caacgttacc acagcaattt atcaaaaaat atcgtcttct
    50521 tttaggcgaa gaagcatccg attttttttc tgcgcttgaa caaggaagcg tgaaaaaagg
    50581 atttcggtgg aatccactaa aaccggcagg attggacatg gtccaaactt accatagcga
    50641 agaattacaa cccgctccct acagcaatga gggatttctt ggcactgtca atggaaagtc
    50701 tttcctccat caagctgggt atgaatatag ccaagaacca agtgcaatga tcgttggaac
    50761 agctgctgca gcaaaacccg gtgaaaaagt tttggatttg tgtgcggctc ccggtggtaa
    50821 atcaacccaa ttagctgctc aaatgaaggg gaaagggtta ttagtgacga atgagatctt
    50881 tccgaaacgt gcgaagattt tatcagaaaa tatagaacga tggggagttt cgaacgcaat
    50941 agtgacaaat catgcacccg cagaacttgt ccctcatttt tcgggctttt ttgatcggat
    51001 cgtggttgat gctccttgtt caggagaagg aatgttccga aaagatccaa atgcaataaa
    51061 agagtggaca gaagaatctc cgctatactg tcaaaaacgt cagcaagaga ttttgtcttc
    51121 agcaataaaa atgctaaaaa ataaaggaca actgatttat tcgacttgta cgtttgcacc
    51181 agaagaaaat gaagagatca ttagctggct tgtagaaaat tatccagtaa cgattgagga
    51241 gattcctttg actcaatccg ttagttcagg acgttctgaa tggggaagcg tagctggttt
    51301 ggaaaaaaca atacgtatat ggcctcataa agatcagggt gaaggacatt ttgttgcaaa
    51361 gctgactttt catggacaaa atcagatgca taaagaaaag aagacacgaa aaaaaagtaa
    51421 agtgcaaatg acaaaagagc aggaaaaact ctggacggag ttttcaaatg attttcatta
    51481 tgaagctact ggaagacttt tagtgttcaa cgaccatctt tgggaagttc ctgagttagc
    51541 cccttctctc gatggtttaa aagtcgtacg tacaggtttg catttaggag atttcaaaaa
    51601 gaatcgattt gaacctagct atgcattagc gcttgctaca aaaaagatag agaatatacc
    51661 ttgcttgccg atcactcaaa aggaatggca atcttacaca gcaggagaaa catttcaacg
    51721 tgatgggaat caaggatggg ttttgctagt attagataag atacctgtcg gatttgggaa
    51781 acaagtaaaa ggaacagtaa agaacttttt ccctaaagga ttgagatttc actgagtgga
    51841 ttagttcgtt tatttttttg tctatgctat gatggagata gataaaaagg agctgatact
    51901 tatgaaaaca tcaacaaaag taacaatcgg attaagcgta gcagccgctg ccagtgtagc
    51961 gacagcagtc gttgtatcgg gaaaagtaat ggaaaagatc catcatatga ctactcggac
    52021 gaaagtaaaa aaattcgtct atgacaaatt tgatggaaat gaaaaattac tagaaatcgt
    52081 agatggtctt tcagatagtg acttggattc tttgatggga atgttagcaa aaatcaaatc
    52141 aggaaaaaag aaaatctctg tttatggtga ttcaatcaaa gattcaaccg aagatgtaaa
    52201 agaccggcta atgcattttg ttgaaaaaat gatgtaagag aaactagctt ttgaacacta
    52261 ttcattcgaa ataaggggtt gtgacaaaat tattgtcaca acccctttaa tctatcttta
    52321 attatgttca tgaagcaata aaagatagca aaacagtaaa tcgttggctt tttttagatt
    52381 gaaaccagac tgttcttgaa atttttcgat tcgatatttt agagtattac gatgaagaaa
    52441 cagtgttttt gcagcagagc tgacattgcc taattcattc cataattctt gaatgatcac
    52501 ttccatttca ttttgtttga tcagttctat gtaacttttg atcaacttac tatcttttac
    52561 agtatctttt gtatagtaat gtaaagccac atcttgcata gaaaaagtac ggctgtgagt
    52621 ataaagattg ttttgctctg ttaaaaaaat ttttctttct tcagcaaaca aagggacgag
    52681 gtcggtgcca ggtgaatgga aagaaccaac gaatacagac gttgtcgtat caaaatcagc
    52741 atctaaagat agaaaaatac cgttgatttc agtagcatca agataatcac ggtttatttg
    52801 ttcgactaag agcgcatctg tgtctgtata aaaaaagaaa tcagcagaat agcgaaataa
    52861 ttccaaaata gtatcttgcc attctttttt taagaattct ccttttgatt cgacgtgaag
    52921 ctggatcaaa cgaaagcttt tttcttgaac acaagaagca tctttaaaaa gatagtgata
    52981 ccatgggtgt tttttttgat cgttgattac agggaataac gctttcaaaa ggctggtttc
    53041 ttgttgagat aatgaatctt gtttgatcca gataaaagca tcatcaattg caaaagaaag
    53101 gatatccgct tctgatgctg gagagttttt tttctgtgct tgtgggtaaa gagcaagtaa
    53161 tttatccatt ttcatcttgt tcacctcttg tatttatttt accatagaaa aaaagttagt
    53221 tacagccttt ttatcatatg ctaaagtaaa acaagaaaaa acaaaggcag ggaagggtaa
    53281 ttggtaaata caaatcttga tgcagtagaa aaagaaaaat ggatgcgcct tgcattggca
    53341 gaagcaaaga aagcagaaat gcttcatgaa gttccgattg gagcagtggt tgtattggat
    53401 ggaaaagtga ttggaagagg atataacctt agagaaacca cgcaagatgc tactacacat
    53461 gcagaaatgc ttgcaatcaa ggaagcctgt gaaaaagtgg gaagctggcg cttggaagat
    53521 gctgcacttt ttgttacctt agagccttgt cctatgtgca gcggcgcgat gattttgtct
    53581 cgtgtagcag aagtttattt cggcgcgtat gatccaaaag gcggaacagc cggtaccttg
    53641 atgaatcttt tggaggacga gcggtttaac caccaagcat atgtagaagg tggcatttta
    53701 gaagacgaat gtgggatgat tttgactgac tttttccgaa agctccgcca gcgaaaaaag
    53761 aaattaaaaa aagactggtc aacgaatgaa aaataaggta tactattctt tgccgaaagg
    53821 ctaggacaat ggtgggcttg tgaagcgtgt caggtctgga aggaagcagc actaagcagg
    53881 ttccgccatg tgtctgatta taatgaaacg acaacagact ttcgggtctg ttttttttat
    53941 ttttatagta ctgtaaaaat gaatgttcct gattttttag ccagactgat ttatcgaata
    54001 ttgtacttat acaatttgct tcaaggcagg aaaaactctg aaatagagct tgtttcaatg
    54061 gaagaaaaag ctgcctatgg tatgataaag ataaagagag gcgagagggt acatggaaaa
    54121 aatagtgatt ttacatacga atgatctgca ttctcattta gaaaattggc caaggatcag
    54181 aaggtttttg gatcaacgta aacgagaaaa cgaaaagaaa gaaaaaacga caacgattgc
    54241 agtagatcta ggtgattttg tggatcgttg gcatccattg tcagaagcaa cgaatggaca
    54301 ggaaaatatc ttgctgatga atgaagttgg ttatgatgca gcgacgatag gaaacaatga
    54361 aggcgtagga aattccaaag aagagttgaa tcatttatac gatcatgctg agtttgatat
    54421 catcctggat aacttattcg ataaaaatac gctccagcca ccggcatgga cgaaaccata
    54481 taaaatcatt acaacgaaac accagacgaa aatcggacta attgcattta ctgctccatt
    54541 tccgcttact tataatccga atggctggga catacggaat ccttatgata ttttgccaga
    54601 attagtcgaa gatttacgtt cacaagtgga tgttttagtt ttgatgagtc atctcgggat
    54661 tcaggatgat ttccaaattg caaaagaaat accagcaatt gatgttattt taggatcgca
    54721 cacccatcat ttattcagag aaggtcacgt aatcaatgat gtccaaatag cagccgctgg
    54781 aaagtacggg cagtatgtcg gtgaagtcca tttaaccatc gatgaagaga aaaaaatcct
    54841 taagcattca gccaaagcca tccctaccga aacaatgaca gcttttacag aagatgaaca
    54901 agaaataaat agttgtctag aacgtgggca tgaattatta aaagaaaaag aagtagcaga
    54961 tttgccattt tctctgagtc tagatatctt caatgagcat tcgtttatcc aagtagcctt
    55021 tgaggcggtg aaagaacgag gaaagacgga agcagcaata ttaaacagtg gtctgttttt
    55081 aactgaaatt ccaaaagggc gggtcaatca ggatcagctt cacactgcgt tgccgcatcc
    55141 gatgcacttg ttaaatgtca cattgggtgg aaatgatctg atccgcttag ttttagaaat
    55201 agaaaaaaat cgtaactttt tacgtaacta tcctatgaag ggaatgggat ttcgcggtaa
    55261 gatttttggg cagatggtct ataatggaat cacatatgat tctattaacc atcaagtctt
    55321 ttggaagaac aagccaatcg atctgaataa aaattatact tttactaccg tagatcattt
    55381 gatgtttgtt ccattttttc caacgattga gattgctgga aaaaatgaat ttttgttccc
    55441 tgaatttatc cgaagtgtgg taggagatta cttgaaagct cactacccaa tcaaataaaa
    55501 acaaggtata ataaaaaaga taggtggtga acgcatgtct gcaaaacaaa atgaacagca
    55561 gaacgttgag cataaaacag aaattgctaa gaaaaaatca tcagctgaac aggaaacaac
    55621 agtcaccgaa gaactgacag ctgctttgga agaaatcgtt gaggagccta aagcagaaaa
    55681 agtcaaaggc gagctagtga cactgttaga tgacagtaat tttttgatag gtgaaagaca
    55741 ttatcggctt gtgtccgatt atcgtgaagg atttaatgca gaaaagcttg gagaacgtta
    55801 cagtgatgta ctagcccgat atgattatat cgtaggagat tggggttatg aacaactacg
    55861 cctaaaagga tttttccaag cggataatcg acgggcgcat ccagatcagc gaatcgattc
    55921 cttggaagac tatctttacg aatattgcaa ttttggttgt gcgtattttg tcatcgagag
    55981 aatcggcggt aaaaaagaaa agacaactca gcgccgaaaa aagaaaaaaa accataatcg
    56041 tgcgtatatc gatgagaaaa aaggaccagt cgcatctaat cgcaaaaaac cggttattaa
    56101 aaatcgaaaa acagagtcag caaaaacctc tgaaaaagta aaaaaacaag aacaacctaa
    56161 acaagtagaa aaaacaaaaa cagcatttac gatcagaaaa agggaagagt agtggatgaa
    56221 tggactataa aggatattta attgatttag acggaacgat ttatcgtggg acacaaccca
    56281 taccggccgg taaacgattt gtggaagaat tgcaaaaaag aaaattacct ttcttgtttg
    56341 ttaccaataa cacgactaag tcaccagaaa cagtagctaa tcgtctagca gatgagtttg
    56401 acattcatgt agctccagag acagtctaca cagctacttt ggcaacaatc gattttatga
    56461 aagcagatgg aaaagggaaa aaagtctatg ttatcggaga agcaggattg attgacttga
    56521 ttttagctgc cggatttaca tgggaagaag aggctcctga ctatgtagtg gtaggcttag
    56581 ataattatct gacctatgaa aaagtagtta aagcaacact tgctattcaa aaaggtgcta
    56641 ctttcattgg aacgaaccca gataagaata tacctactga acgcggcctt cttcctggag
    56701 cagggtcagt catctctttt gtagagacag caacccaaac gccgccgatc tacatcggaa
    56761 aaccagaagc catcattatg gataaagccg ttgaagtatt agggctgcaa aaagaagaag
    56821 taatcatggt tggagataac tatgaaacag atatccaagc tgggatacgc aataatatag
    56881 atactttgct cgttttatca ggttttacaa aagaagaaga tgtacctggg ttgcctatcc
    56941 cagctaccta tgtgaaacaa tcactggatg aatggagctt ctaatatgaa aaattcgaga
    57001 tggcagtgga tggaatatgc tggattgttc agcctatttc tgacactgat cagtctggca
    57061 gttggtgtga cgatcaactt tcgaccacta tacgtatttg atatcggaca tttgcaaatt
    57121 ttggattata cttctttaga tcaagagacg ttactgaaaa attttgatca cctaatgaat
    57181 tatctcaata atcccttcaa aaccatcctc agcttgccag atttcccagt ttctgctagc
    57241 ggagcccatc acttttatga agtgaaaatt ttgtttttag tggattatgc tgtgtttttc
    57301 atcactttga tcccgagtat tttgtttatc aaatatttgc aaaagaatga tcggctttgg
    57361 cggttgattc gtccgtttca aatcggcatg ttactgccag ttgttttcgg ttttttcatg
    57421 atgataggat ttgatcgctt ttttatcctt tttcatgaaa cgtttttcaa caacgatgac
    57481 tggttattcg atcccgttac tgatccaatc atcaatgtac ttccagaaga atttttcatg
    57541 catagcttta tcttattttt tgtattatta gaattatttt ttgctgtttt tcttttctta
    57601 ggaaaaaata gcttgaaaca aacaaaaaag aaagaactcg tctaaataga cgttttcttt
    57661 ctttttttgt tgaattattg agtaaggcga cttctttttt ctggttcttt gtgtgctaat
    57721 cgactggcag cagcagcggc gatcgctccg acgatatcat ctaagaaggt atggacatga
    57781 acgccatcgt ggctgtttag ttcttttaaa ataccaggtt tcaccttgtc gatataacca
    57841 taattcgtaa agccgatcgt accgtaaaca ttcacgattg acaaggctag gatctcatcg
    57901 ataccgtaaa ggccttcgtc atcttcgata atatgttgca acggactcat caattttttc
    57961 ttttctgcta atatatctaa ttggattcca gtgataatgg cgttatgtac ttcacgcttt
    58021 gttagcacgc tgttgacact ttcagtacaa tccgccaaag tcaaatcaga aatatagtct
    58081 ttttgtaaaa acatgacaag ttgtgcgata tcttctacag ttacgccacg ttccttcaat
    58141 agttctcttg ccttttcttc taaagtctca gttttgacga ccatattatt gctccccctt
    58201 tattttttct ttctctattt catgttaacg gaacatgaaa aaaatgcaag aaactgcttt
    58261 cgggtttaac ttgaaatgca aagagatata aaaaatcaac taggacgaag atttttatcg
    58321 tataagcttg tgctgtgacc aggctgagtt cgttcctttg gattaatata gtgtaaagcg
    58381 ttgttcactg cagtaggtgc ttcaccgagc ccagtagcaa tcagtttcac ttttccttca
    58441 taggtacaga tgtcaccagc tgcataaacg cctggaatag aagtagacat atctgatttt
    58501 acagtaatgg catttctggt actatcaagt ccccaagaag aaagatgttc taaattagaa
    58561 gagaatccat aattgacgat cagagaatca atcatcaaat caatcgtctc gtcacttttc
    58621 acttttttta accggatatc tgtcagttcc ccattattgc ctgataatcc atcgatgaga
    58681 tagggagtaa gcaagttgac agatgaagat ttcaagcgtg atacactatg ttcgtgcgca
    58741 cgaaattccg ggcgacgatg aatcaagtaa acttcgcttg caataggctc taacatcaac
    58801 gtccaatcga tagcagaatc accacctcca gcaatagcga ctttttttcc agcatacttc
    58861 atcaaatcat tcacgaaata atccaatccg tggttttcaa atgattcagc attatctaaa
    58921 ttcaattttc ttggctggaa tgaaccattt cctaaagcta aaatgactgc tttggaataa
    58981 tggatgcctt tatttgtagt aatctcgata acttcgtctt cacgagtaag cgagagaact
    59041 tcttctttta aatggaatgt atggttaaaa gtagttaatt gtttttctaa ttgatcgatt
    59101 aattcacttg cttttattgc tggataccca ggaatatcgt aaatatattt ttcgggatac
    59161 aaagttgcta attgaccacc aagttgaggt agactgtcaa tgatttttgt tttagcttgg
    59221 cgcattcctg cgtaaaaagc ggcaaacatc cctacaggac ctgcaccgat gattgtaatg
    59281 tcatagattt ccataaaaat tcctcccttt tcacactata tcagaattta attgagaatg
    59341 cttctcaaaa atgtttgaat tcgaaatcga aacggtaagt tatagaatgt atctagtgat
    59401 gaaaaataaa agaaaacaaa tctagtattt ttacgagagg ttaggtatta tgttattatt
    59461 attatgaagt atgcaaatat taggaggaaa caatatggca ttcccacaac tagatttaaa
    59521 aagtgaaaaa ggaccaaaag ctgttatcaa aacaaaccgt ggtgacatta ctgttcaatt
    59581 attccctgaa ctagcaccga agacggttca aaatttcatt gaactttcca aaaaaggtta
    59641 ttatgatggc gtgatttttc accgcgttat tcctgatttc atgatccaag gcggcgatcc
    59701 aactggaaca ggaatgggtg gcgaaagtat ttatggcgaa agttttgaag atgaattcag
    59761 tcgtgaactg ttcaaccttc gcggagcttt atctatggca aattcaggtc caaacacaaa
    59821 cggaagccaa ttctttattg taaataacac aaatgtgcca gctaatatgc ttggacaact
    59881 agaaggtgct ggattcccat cagaaatcat tgaagcgtat aaaggtggcg gaactccttg
    59941 gctagatttc cgacatacag tctttggaca tgtactagaa gggatggaca cagtagatga
    60001 aatcgcaaat gtacaacgtg gtccacaaga ccgacctgta catgatgtag tgattgaaac
    60061 aattgaaatt tcagaatagt ttggtatggt acggagtaat tatgaaaaag gacgaaacct
    60121 agtaaggtct cgtcctttta ttttgaataa atgatttaat cgattgtctc taaagcatac
    60181 ttgattcgat taagtccttc cagtaaagtt tcttctggac aagcaatatt caagcgcata
    60241 tgcccatgac cggaaggacc aaacgtgatg ccaggattta atacgacttt tcctttccga
    60301 acaagagtgt tctccaattc ttggtcgtct gaaatatagg cggtaaaatc taaccacatt
    60361 aagtaagttc cttcagggtt cattattctc actttcggaa gatactgttc aaaaaactga
    60421 ttaactgttt gaacattttt ttctagataa ggaatcagtt ggcttaacca ttctccaccc
    60481 gtctcataag ccgcttgggt tccaatcagt ccaaaagtgt tgatttcgtg ctgttggttc
    60541 atcactaaat gtttttcaaa atcagctttc atctctggat ttttgataaa taccattgaa
    60601 tttttgattg cagctagatt gaaagtcttg gttgcggaag taaaagcaac tagtagatca
    60661 tctaaattag gatcaatcgt tagcatggaa gtaaatgtat gatcgaataa agttaaatct
    60721 tgatggatct cgtcactaaa cagaatcact tgatgtttta aacacaattc gcttaactgt
    60781 ttcaattctt ctttgctcca aacacgccct ccaggattgt gaggattgca taaaatcatt
    60841 gcttttacat tgttttcttc gatttttttc tccatgtcag acaagtccat gatgaaatga
    60901 ttattttttt ctagcaaaga gcttcgaacg atgtgacgtt gatttgtttc tataatagag
    60961 gaaaatggag gataaactgg gtcatgaatc aaaatagaat cgcctggttt tgtaaaagtt
    61021 tgtactgctg cagctaaact ggttaacaca ccacttgtaa aaacgatatt ttctttcgtc
    61081 aactccacag catggtgttg gcgttcccaa tgaatgatag cctgatatag tttatcagac
    61141 agagtggcat aaccaaagat tccatgtttg atgtattcac taaacgcttt agctacccca
    61201 ccgggagaca agaagtccat atctgctacc cacaaaggaa gtaagtcatc catttgatat
    61261 gtctcagcaa ttgcgtccca ttttacactt tctgtatctt ttcttgaaat ctgtttatca
    61321 aattcgatca tcttaagacc tccagtcatt ttcgtcgctt ctttttttga gctatttaca
    61381 gtataatgat aaatgcgaag agagggaagt agatgactta taaaattgga caaataattg
    61441 aaggaaaagt gacagggata cagccttatg gcgcattcgt ttctcttgat aacgagacgc
    61501 aaggacttat tcatgtttca gaagtacaat ccggttatac gaaaaatatc cattcattgt
    61561 taaaagtcgg acagccagta acagttcaga tcatcgacat tgatgaatat tccaaaaaaa
    61621 tcagtttatc tttaagaacg ttagaaaaaa caaatccagc tgttccatat cgcagaaaaa
    61681 ggtatttcac gaataaaaat cgtaagatcg gatttaccac tattgcggag cagttaccta
    61741 tttggataga agaagcatta tctgaactag aacggaaaga aaataagaaa taaaaagtgt
    61801 attttttctg aaaatgtgct agaattttta tgtaagccat agaacaagtt gatcgaggtg
    61861 gaaattttgg caagcaaaag agttgctggt acaattatgt tgcatttgga ggatggtact
    61921 aaaaaatttt tggtccattc aattgatgac aagttggaat ttgcgttagc ggaactttct
    61981 gaaggaaaga ctggtttagc gaatatactt aatttcttaa aagaaatagt tcatatcgat
    62041 gttagcaaga ttagtttgat ggaattgaca aatacacata ttaaagagga aaatgttccg
    62101 ttgtttgtct ttgaaactga acaaaagaat caaagagaag aattaggcga aggatatatt
    62161 tgggaagaac caggtcaaat gcgctctgtt cttggaactt acgaagtaga gggagtgcct
    62221 ttcttctaaa atacagatta agcgaacaaa gaagtttgaa tttttttcaa acttcttttt
    62281 ttattgtttg gtccaaaaaa tacaatgaaa tgttctgcta aagatagttt tttagtgcat
    62341 tttattttca aaatatgttg aaaacggttg tataacgaat aataataatt gatcgaaaaa
    62401 tcatttcaca aagtaatgct taatttatag ttgaccaagg aagaaataca tgataaattt
    62461 aacagcgttg attctgaagt ggatgttata tcacatttct aaaaaattta aaaaatagtt
    62521 attgacatgt tactaacgac atgataagat aactgagtcg ttaaaaaaga accacttgcg
    62581 aagcttgatt ctatcgaaaa aaacttcaaa aaagttcttg acaaataaca aacgataagt
    62641 tatgataaat gagttgctga aagatttcga aaaacgaaat cgaaaaaaac ttttaaaaaa
    62701 gagttgacaa tcgcttgtct ctctgatatg atataaaagt tgctacggca gccaaaaacg
    62761 aatcgggaaa cactc
//
LOCUS       DANKHJ010000008        61702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000008 DANKHJ010000000
VERSION     DANKHJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(122..499)
                     /locus_tag="PW113_000610"
     CDS             complement(122..499)
                     /locus_tag="PW113_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524945.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="PW113_000611"
     CDS             755..1483
                     /locus_tag="PW113_000611"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2524946.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="PW113_000612"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="PW113_000612"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2524947.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="PW113_000613"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="PW113_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2524948.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="PW113_000614"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="PW113_000614"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2524949.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="PW113_000615"
     CDS             4618..6057
                     /locus_tag="PW113_000615"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2524950.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /locus_tag="PW113_000616"
     CDS             6080..7132
                     /locus_tag="PW113_000616"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2524951.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="PW113_000617"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="PW113_000617"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2524952.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="PW113_000618"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="PW113_000618"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2524953.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="PW113_000619"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="PW113_000619"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2524954.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="PW113_000620"
     CDS             10651..11565
                     /locus_tag="PW113_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2524955.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="PW113_000621"
     CDS             complement(11698..13263)
                     /locus_tag="PW113_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2524956.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="PW113_000622"
     CDS             complement(13256..14707)
                     /locus_tag="PW113_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2524957.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="PW113_000623"
     CDS             complement(14721..15737)
                     /locus_tag="PW113_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2524958.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="PW113_000624"
     CDS             15957..17045
                     /locus_tag="PW113_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2524959.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="PW113_000625"
     CDS             17099..18625
                     /locus_tag="PW113_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2524960.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="PW113_000626"
     CDS             18627..19832
                     /locus_tag="PW113_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2524961.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="PW113_000627"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="PW113_000627"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2524962.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="PW113_000628"
     CDS             21330..22754
                     /locus_tag="PW113_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2524963.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="PW113_000629"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="PW113_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2524964.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="PW113_000630"
     CDS             24327..25304
                     /locus_tag="PW113_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2524965.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="PW113_000631"
     CDS             25329..26972
                     /locus_tag="PW113_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2524966.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="PW113_000632"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="PW113_000632"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2524967.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="PW113_000633"
     CDS             28548..28823
                     /locus_tag="PW113_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2524968.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="PW113_000634"
     CDS             28829..29596
                     /locus_tag="PW113_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2524969.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="PW113_000635"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="PW113_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2524970.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="PW113_000636"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="PW113_000636"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2524971.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="PW113_000637"
     CDS             33740..34042
                     /locus_tag="PW113_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2524972.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="PW113_000638"
     CDS             34045..35208
                     /locus_tag="PW113_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2524973.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="PW113_000639"
     CDS             35234..38662
                     /locus_tag="PW113_000639"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2524974.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="PW113_000640"
     CDS             38683..39807
                     /locus_tag="PW113_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524975.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="PW113_000641"
     CDS             39797..40234
                     /locus_tag="PW113_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2524976.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="PW113_000642"
     CDS             40218..40556
                     /locus_tag="PW113_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2524977.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="PW113_000643"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="PW113_000643"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2524978.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="PW113_000644"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="PW113_000644"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2524979.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="PW113_000645"
     CDS             41713..42756
                     /locus_tag="PW113_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2524980.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="PW113_000646"
     CDS             42915..43643
                     /locus_tag="PW113_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2524981.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="PW113_000647"
     CDS             43759..44004
                     /locus_tag="PW113_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2524982.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="PW113_000648"
     CDS             44001..44219
                     /locus_tag="PW113_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2524983.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="PW113_000649"
     CDS             44355..44894
                     /locus_tag="PW113_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2524984.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="PW113_000650"
     CDS             44887..47160
                     /locus_tag="PW113_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2524985.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="PW113_000651"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="PW113_000651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2524986.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /locus_tag="PW113_000652"
     CDS             complement(48333..48587)
                     /locus_tag="PW113_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2524987.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="PW113_000653"
     CDS             complement(48987..49256)
                     /locus_tag="PW113_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2524988.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49726..50181)
                     /locus_tag="PW113_000654"
     CDS             complement(49726..50181)
                     /locus_tag="PW113_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2524989.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="PW113_000655"
     CDS             complement(50319..52247)
                     /locus_tag="PW113_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2524990.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="PW113_000656"
     CDS             complement(52444..53277)
                     /locus_tag="PW113_000656"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2524991.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="PW113_000657"
     CDS             complement(53469..54173)
                     /locus_tag="PW113_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2524992.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="PW113_000658"
     CDS             complement(54196..54822)
                     /locus_tag="PW113_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2524993.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="PW113_000659"
     CDS             55148..57523
                     /locus_tag="PW113_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2524994.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="PW113_000660"
     CDS             complement(57547..58038)
                     /locus_tag="PW113_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2524995.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="PW113_000661"
     CDS             58225..59145
                     /locus_tag="PW113_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2524996.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="PW113_000662"
     CDS             59299..59643
                     /locus_tag="PW113_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2524997.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="PW113_000663"
     CDS             complement(59833..60333)
                     /locus_tag="PW113_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2524998.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="PW113_000664"
     tRNA            60861..60944
                     /locus_tag="PW113_000664"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
     gene            complement(61268..>61702)
                     /locus_tag="PW113_000665"
     CDS             complement(61268..>61702)
                     /locus_tag="PW113_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2524999.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20233 a        10662 c        13216 g        17591 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    61261 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    61321 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    61381 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    61441 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    61501 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    61561 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    61621 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    61681 ttgttaattg ttgtcctgag ta
//
LOCUS       DANKHJ010000009        60492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000009 DANKHJ010000000
VERSION     DANKHJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            247..1026
                     /locus_tag="PW113_000666"
     CDS             247..1026
                     /locus_tag="PW113_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525000.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1072..2016
                     /locus_tag="PW113_000667"
     CDS             1072..2016
                     /locus_tag="PW113_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2525001.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2032..2811
                     /locus_tag="PW113_000668"
     CDS             2032..2811
                     /locus_tag="PW113_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2525002.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            2823..3521
                     /locus_tag="PW113_000669"
     CDS             2823..3521
                     /locus_tag="PW113_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2525003.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            3549..4313
                     /locus_tag="PW113_000670"
     CDS             3549..4313
                     /locus_tag="PW113_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2525004.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4326..6143
                     /locus_tag="PW113_000671"
     CDS             4326..6143
                     /locus_tag="PW113_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2525005.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6154..6786
                     /locus_tag="PW113_000672"
     CDS             6154..6786
                     /locus_tag="PW113_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2525006.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            6824..7906
                     /locus_tag="PW113_000673"
     CDS             6824..7906
                     /locus_tag="PW113_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2525007.1"
                     /translation="MSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEEELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPVFVDSDYETWNMN
                     PEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGTDVAQTLFEKGIC
                     LPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            7914..8528
                     /locus_tag="PW113_000674"
     CDS             7914..8528
                     /locus_tag="PW113_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2525008.1"
                     /translation="MYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGSPVIFK
                     QQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPELWNIL
                     KGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFEKDIEY
                     VENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            8521..9681
                     /locus_tag="PW113_000675"
     CDS             8521..9681
                     /locus_tag="PW113_000675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525009.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNA
                     EKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGIN
                     YNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
     gene            9671..10567
                     /locus_tag="PW113_000676"
     CDS             9671..10567
                     /locus_tag="PW113_000676"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2525010.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            10539..11630
                     /locus_tag="PW113_000677"
     CDS             10539..11630
                     /locus_tag="PW113_000677"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HDL2525011.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISLSSYIFSINMLASTS
                     IVFLLISNNFIRIIQNIFVLNYIIYASVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFI
                     NIRGTAFVNGILPLFRENIIFKAVYYFFE"
     gene            11667..12605
                     /locus_tag="PW113_000678"
     CDS             11667..12605
                     /locus_tag="PW113_000678"
                     /inference="COORDINATES: protein motif:HMM:NF016640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HDL2525012.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            12851..13867
                     /locus_tag="PW113_000679"
     CDS             12851..13867
                     /locus_tag="PW113_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HDL2525013.1"
                     /translation="MKMKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQL
                     KEDRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAY
                     IDSNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKK
                     SNELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKME
                     RDFTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFV
                     ETIEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTV
                     YYLS"
     gene            14235..15659
                     /locus_tag="PW113_000680"
     CDS             14235..15659
                     /locus_tag="PW113_000680"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2525014.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            15889..16173
                     /locus_tag="PW113_000681"
     CDS             15889..16173
                     /locus_tag="PW113_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525015.1"
                     /translation="MSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANT
                     LVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            16261..17427
                     /locus_tag="PW113_000682"
     CDS             16261..17427
                     /locus_tag="PW113_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2525016.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            complement(18810..19076)
                     /locus_tag="PW113_000683"
                     /pseudo
     CDS             complement(18810..19076)
                     /locus_tag="PW113_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19914..20642
                     /locus_tag="PW113_000684"
     CDS             19914..20642
                     /locus_tag="PW113_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2525017.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            20660..21853
                     /locus_tag="PW113_000685"
     CDS             20660..21853
                     /locus_tag="PW113_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2525018.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            21846..22841
                     /gene="lacD"
                     /locus_tag="PW113_000686"
     CDS             21846..22841
                     /gene="lacD"
                     /locus_tag="PW113_000686"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2525019.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            22838..23779
                     /locus_tag="PW113_000687"
     CDS             22838..23779
                     /locus_tag="PW113_000687"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2525020.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            23825..25591
                     /locus_tag="PW113_000688"
     CDS             23825..25591
                     /locus_tag="PW113_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDL2525021.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            25608..26087
                     /locus_tag="PW113_000689"
     CDS             25608..26087
                     /locus_tag="PW113_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDL2525022.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            26100..26990
                     /locus_tag="PW113_000690"
     CDS             26100..26990
                     /locus_tag="PW113_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2525023.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            26977..27786
                     /locus_tag="PW113_000691"
     CDS             26977..27786
                     /locus_tag="PW113_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2525024.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            27802..28206
                     /locus_tag="PW113_000692"
     CDS             27802..28206
                     /locus_tag="PW113_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2525025.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(28387..29325)
                     /gene="yidC"
                     /locus_tag="PW113_000693"
     CDS             complement(28387..29325)
                     /gene="yidC"
                     /locus_tag="PW113_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2525026.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(29659..30741)
                     /locus_tag="PW113_000694"
     CDS             complement(29659..30741)
                     /locus_tag="PW113_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2525027.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            30883..32334
                     /locus_tag="PW113_000695"
     CDS             30883..32334
                     /locus_tag="PW113_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2525028.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            32327..33049
                     /locus_tag="PW113_000696"
     CDS             32327..33049
                     /locus_tag="PW113_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2525029.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            33069..34220
                     /locus_tag="PW113_000697"
     CDS             33069..34220
                     /locus_tag="PW113_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2525030.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(34568..34843)
                     /locus_tag="PW113_000698"
     CDS             complement(34568..34843)
                     /locus_tag="PW113_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2525031.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            34951..35715
                     /locus_tag="PW113_000699"
     CDS             34951..35715
                     /locus_tag="PW113_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2525032.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            35839..36342
                     /locus_tag="PW113_000700"
     CDS             35839..36342
                     /locus_tag="PW113_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2525033.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            36607..37254
                     /locus_tag="PW113_000701"
     CDS             36607..37254
                     /locus_tag="PW113_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2525034.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            37415..38683
                     /locus_tag="PW113_000702"
     CDS             37415..38683
                     /locus_tag="PW113_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2525035.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(38860..40062)
                     /locus_tag="PW113_000703"
     CDS             complement(38860..40062)
                     /locus_tag="PW113_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2525036.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            40589..41314
                     /locus_tag="PW113_000704"
     CDS             40589..41314
                     /locus_tag="PW113_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDL2525037.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            41304..41978
                     /locus_tag="PW113_000705"
     CDS             41304..41978
                     /locus_tag="PW113_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDL2525038.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(42266..42550)
                     /locus_tag="PW113_000706"
     CDS             complement(42266..42550)
                     /locus_tag="PW113_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HDL2525039.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(42844..44151)
                     /locus_tag="PW113_000707"
     CDS             complement(42844..44151)
                     /locus_tag="PW113_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2525040.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            44640..45746
                     /locus_tag="PW113_000708"
     CDS             44640..45746
                     /locus_tag="PW113_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525041.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            46337..46537
                     /locus_tag="PW113_000709"
     CDS             46337..46537
                     /locus_tag="PW113_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2525042.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            46684..49473
                     /locus_tag="PW113_000710"
     CDS             46684..49473
                     /locus_tag="PW113_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2525043.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            49520..50047
                     /locus_tag="PW113_000711"
     CDS             49520..50047
                     /locus_tag="PW113_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2525044.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            50068..51258
                     /locus_tag="PW113_000712"
     CDS             50068..51258
                     /locus_tag="PW113_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2525045.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            51298..52596
                     /gene="asnS"
                     /locus_tag="PW113_000713"
     CDS             51298..52596
                     /gene="asnS"
                     /locus_tag="PW113_000713"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2525046.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            53530..54204
                     /locus_tag="PW113_000714"
     CDS             53530..54204
                     /locus_tag="PW113_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2525047.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            54201..54542
                     /locus_tag="PW113_000715"
     CDS             54201..54542
                     /locus_tag="PW113_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525048.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            54572..54931
                     /locus_tag="PW113_000716"
     CDS             54572..54931
                     /locus_tag="PW113_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2525049.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            55455..57536
                     /gene="cadA"
                     /locus_tag="PW113_000717"
     CDS             55455..57536
                     /gene="cadA"
                     /locus_tag="PW113_000717"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2525050.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            57847..59313
                     /locus_tag="PW113_000718"
     CDS             57847..59313
                     /locus_tag="PW113_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2525051.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            59623..60096
                     /locus_tag="PW113_000719"
     CDS             59623..60096
                     /locus_tag="PW113_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDL2525052.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFIVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            60170..>60492
                     /locus_tag="PW113_000720"
     CDS             60170..>60492
                     /locus_tag="PW113_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2525053.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEA"
BASE COUNT        20214 a         9952 c        12388 g        17938 t
ORIGIN      
        1 tgaaaattaa ggaaataaaa atgaaatcga ttaattgtta tcttattaat gttttattta
       61 cttttaaatc gtaaattgcc ctagtcaaga aaacaatttg tggtatgatt atatagggaa
      121 aacttgttta caaatatttc tgtgaatata ttaatttaat tatgtttttt aaaataaaaa
      181 gctgtcattt ttatgtatgt gaattatatc ttaatttatc tatcatcaat acttaggagt
      241 gaatctatgg aaaagccatt cgtcaagtca ttgactgctg tggcggtagg tgcaggtgct
      301 attgcgatct gtttgtttgg ttaccatatc aataatcagc ggcaacatca gcaacggatc
      361 aattatgcag aatctgccat caccaatcaa aaagacacct tgacttcttt atcaaaagaa
      421 gtagacaagc tgtatagtac aaaagaaaaa aattttctaa accctgaaat aacagaagaa
      481 acaatcacaa gtttgtcaaa taagctatcc tctgtcaaac tttcagcaga tgattacgac
      541 atcaaagaat cagaattgcc taaagaagct gttgcaatcc aagatgagaa gaaagcaatc
      601 gtgacacagt tagatagtgc agaatcaaaa ctgaagatcc agacagctgt caacgattta
      661 tttactaaaa atgtatcaaa ctggcagcaa gctgtagacg atgtcgtcat caaagaaaag
      721 gtgacaagtg cagatgtcgc tcgtgtacgt gaaaatatga gcttttttaa agatagtcct
      781 tggaaaacgg ttgttatgca atatctagga ttcgctgata ctcagatcgc gcaagtcacg
      841 caactagatc agttattcga tacgatgttg aaggatggtc aagtgactgc caccgctact
      901 tacgatcaat atttgacggc gctcagtcaa atagagcaga tccggaatga aaaaataagc
      961 gcagcatacg caacaaaagc acaaactgtt gctcagcaaa tgggctatag caatataagt
     1021 tattgacagt taaaacgttg attcaagcaa ggttagcaaa cggaggaaac tatggcaaga
     1081 cgaagaagaa aatcaagagg gaaaaaaata tttctgatta ttttagcagt tcttatcgta
     1141 ttagtattag caattgtagg agtaggagca aaattatatc tggatctctc acattctatt
     1201 gagaaaactt acgaatcagt ggaacgaacc caaggagaac gaacgaaacc agttgatctg
     1261 gacaaacaag aatctttctc tgttctattg atgggcattg atactggaga tttaggtcgg
     1321 gtcgatcaag gaagatcaga tacgatgatg gttgccacag tcagtcctga agacaaacag
     1381 acaacgatcg tcagcattcc tcgagatacg tatgtcgaca tcgtaggtcg tgacacaaaa
     1441 gacaagatca accacgctta cgcattcggc ggagctgcca tggcgatgga taccctacaa
     1501 aaatatgtag acatcccaat cgatcactat gtctctatca atatgaaagg gctaaaggaa
     1561 ctcgttgatg cagttggtgg tatcgaagtc aataatgacc tcactttctc acaagatggc
     1621 tatgactttc caatcggtaa aattacattg aacggggatc aggcattatc ttattctcgt
     1681 atgcgttatg aagatccaaa tggtgactat ggtagacaag agcgtcaaag aaagatcgta
     1741 gaaggtgtag caaaaaaagt attgagttta aacggtgtca gcaactatca atccgttttg
     1801 aatgctctag agtcgaatat gaaaacagac atgagctttg atgaaatgaa aaaaatcgct
     1861 ttagattatc gggaggcatt tggtacgatc aagcaagatc agctgcaagg agaaggcttc
     1921 atgcaagata atatctccta tcaaaaagtc agcgatcaag aacttgatcg tgtgaaaaaa
     1981 gaattaaaag atcaaatgaa tctagagaat aaataggaaa gtggagaaaa aatggaagag
     2041 acaatcagct tgaaagaaat ttttgaaaca ttacgcaaac acttggtaac aatcatcatc
     2101 tcaatgtttg ctggcttggc catctcagga atcgtaacat ttttcatctt gacaccaaaa
     2161 tacagttcac aagcacaatt gatcgtgaca ctgccacaat cagacacagc aaatgtcaat
     2221 gatgtcaata cgaacctgca aatgatcaat acgtataaag atatgatcgt cagtgattta
     2281 gtactaaatg aagtcaaaga tcgtttagaa acagaagaca atgtgaaaat gactgctgga
     2341 caaatcaaag atgcgatctc cgtcaatcag tcacaaaatt cacaaatgtt ctcgatccag
     2401 gcaataagca cgaatgcagt gactgctcaa cagatcgcta atacaacttc acaagttttc
     2461 caagaaaacg ccaaagacgt gatgaatgtc gataaaatct cgattatttc aggtgcagtt
     2521 gccaatacga cacctgtttc gccaaataat aaattgaatc tagtgattgg cttagtgtta
     2581 ggaatgatgg taggcgtagg tctggcattc ttattcgaat tacttgatcg gacagtgaaa
     2641 gatgaaaaat atgtgacgga tacattagga ttcccaatct taggaacagt tccagaaatg
     2701 tcagcaaaag aattgaacgc tggtatccgt aaaaaaggat cagctcctgc acgaccaaaa
     2761 gctacaaacg ataaaacagc cccatcacgc agaagccgct caagagtcta ggaggaagaa
     2821 aaatggcacg aacacagaaa caaaaagcaa ctcaaactca tgcagtcagt ttgatcacat
     2881 taacaaaccc atcttcacca atcgccgaac aatatcgtac gatccggacg aatatccagt
     2941 ttgcctccgc agctggtcag caaatcaaaa cgattgtcgt gacttcatca ggtcctggag
     3001 aaggaaaatc caccactgca gcaaacattg ccgttgtttt tgccaaatct ggtcaacggg
     3061 tactgctagt cgatgcagac ttacgtaagc ccgtcatcta taaaacgttc aagttgaaca
     3121 acgcaagcgg attaagtact gttcttagtt catcaggaag tgtagcagat gagatccaac
     3181 gaacaccagt cgacaatttg tcgattctgc caagtggacc taaaccacca aatccatcag
     3241 aactactaag ttcgccaaga atggatcaga ttttagcaga agcaagacaa ctatttgatg
     3301 tagtgatttt cgacatgcca ccagtcgttg ccgtaacaga tgcacagatc atgtcttcaa
     3361 aaacggatgg caccttattg gtcgtacggg aaaatacgtc acgaaaagaa tcattgacta
     3421 aagcaaaaga actgttagat atggtacaag cgcgagtcct tggtgttgta tacaatggag
     3481 cagaacacag cagagatgct ggctattact acgggaatta agaaataaaa agagaggaga
     3541 caagagaaat gatcgatcta cattgccata tcctgccagg aatcgatgac ggagcagaaa
     3601 acatagatgc cagtatcgcc atggctgaaa aagcgattca acaagggatc acacatattt
     3661 tatgcacacc ccatcacaat aatggaaaat acagcaatga aaaatcacaa gtcatttctc
     3721 tagtcgcttc gttacaagca gaattagaga aaagacaatt gcctttgacg ttattggaag
     3781 gacaagaagt acgtattacc ggaacactga ttgaagacat ccaccgagat gaaattttat
     3841 ttacagattt agatgataca tatcttctaa tcgaatttcc gacactggaa gtgccgttat
     3901 atgcagaatg gctctttttt gagttgttac aactaggaaa gacaccagtg atcgtccatc
     3961 cagaacgcaa cgcgcatttt cgagaagacc caaatcattt gctgccattc ctagatatgg
     4021 gatgcttggc tcaactcacc gcaccaagtt atgtcggtgt atttggaaaa gacatccaga
     4081 aaacagccaa aatgatggtc aaacataacc tcgtgcaaat ggctgcttcc gatgcccatg
     4141 gggtctctaa acgtaccttt tacttaaaag aatgttatga gcaaatcgcc aaggactttg
     4201 gtaaggaaaa agtagcacag atgaaacaag tcgcaaagga cctgatcaac ggggatgaga
     4261 tcagttatcc agtctatgaa gaggtaaaaa agaagaagtt tggtttattt tagggggaat
     4321 gtagggtgtt tgtattaaca agaagaagaa aaattgccac attggtattt gtggacagct
     4381 tgttgttgat tgctgcgaat attgcagcag ctaaattcat gaagccgttt gtagctatac
     4441 caatggacct tattttaatc tctatcggtt tgtcgattgg tttttattta ttctatggta
     4501 gtttattcaa agtttttact cgaatcaatc gttatacaaa tttaagagag atcattgcga
     4561 tttttggttc gttatctgca agtgcggcct caagtatcat catattgctt ttcataaata
     4621 aacaatacag tttacgttta gtgatctttg catacctact ttcgctatta ctgatcattg
     4681 gcagccgatt gatctggcgt atctatatcg aaacaaaaaa tatgaggtat ctatcaacag
     4741 attccgctaa aaataccttg atcgttgggg ccggcgaagg tggacgtatt ttatacaaca
     4801 gctttcttgg ttctaaaacg gctcaagata tccatgtcat tggattcgtg gatgatgatc
     4861 cgaataaaag aaatacctat ctttcaggaa aaaaagtact ggggtcatta aaagatatac
     4921 cggaattgat cgaaaaatat gacatccaaa tggtaacgat cgctatccct tctttatcaa
     4981 gaaaaaaatt acgaagaatc ttcgagttag tcgaatctgc tcatgtgaaa gtcaatacga
     5041 tgccttctat ggaagaactt gcctcaggga aaatcagcgt atcaaaatta aaaaccatcg
     5101 acgtagtgga attactggga agagatgagg tagagttgga tatcgaatcc atcaaagacc
     5161 agatcacaaa caaagtcatt cttgtcaccg gtgcaggagg atcaatcggt tcagaaatct
     5221 gccggcaaat catccaattt gaccctgcaa aactattact tttaggtcat ggggagaact
     5281 cgatttactt gattgaccgt gaattacgca cgcatcatca aaagtgtcct acagaaatcg
     5341 tgccgatcat tgcggatatc caagatcgca agaaaatcaa tgagatcatg gaacaatacc
     5401 acccagatat cgtctaccat gcagccgctc ataagcatgt accattgatg gaatataacc
     5461 caaaagaagc agtcaaaaac aatattttcg gtacaaaaaa tgtagcagaa gcagccaaag
     5521 cagcgaaagt aaaaaacttc gtcatggtct cgacagataa agccaacaat ccgccaaacg
     5581 tcatgggttc aacaaaacga atcgctgaaa tgatcgtgac tgggttgaat gaagaaggat
     5641 gtacgaaatt ctcagctgtc agatttggga atgtccttgg ttctcgcggt agtgtgattc
     5701 cagtattccg ggaacaaatt gctcaaggag ggccagtcac tgtaacagac tttcgtatga
     5761 cccgatattt catgacgatt cctgaagcaa gtcgtttagt tattcaatct ggggcgttag
     5821 caaaaggcgg cgaaatcttt attttagata tgagtgaacc agtgaaaatc gtagatctag
     5881 caaaaaacat gatccgattg agcggatata gcgaagacga gatcgaaatc attgaaacag
     5941 gtatccgtcc aggggaaaaa ctatatgaag aactattgtt agataaggaa agaaacgatg
     6001 aagcagttta cgagaagatc tttgtgggaa acatcaaagg ttattccatg caaacagtga
     6061 tggaatttgt caagagcctg ccgcaagacg atgaacaatt ggctaaagac attgtcactt
     6121 ttgcgaatgc ttcgaataag tagggggagt aaagtgaaag caattattgt aggagctggt
     6181 ggatttggca aagagattgc ttttctacta gaaagtttct ctagatatga attgattggt
     6241 tttgtcgatg attcgttaaa aatgcaaaat caagaattag tgggaaaaac tgtattggga
     6301 acaatagact cgttattaga attagaagaa gaaactgcta tttttctggg aatcgcttcg
     6361 ccagatatca aagagaaaat atatcaaaag ataaataaaa ataataaatt gattttccct
     6421 aatttagttg catcaagtgc actagtgggc ataaatgttc agttaggaat tggcaatatt
     6481 ttgatgccct atacgactta cactgctgac gtagttttag gaaatttcaa tatgatcaat
     6541 atcggctcta ctatcggaca tgatactcaa atcggtaatt ataattcaat ttttccaagt
     6601 gtaaatattt caggacatgt tctattagga gataaaaatg aaattggagt aggtactaaa
     6661 ataatacaaa atctaagaat tggaaatatg aacattatag gtgcgggaag tgtagtaata
     6721 aaaaatatta ataataagac taaaaatgtt ggcgtaccta ccaaagtcat agaaagatgg
     6781 gattaagttg gaaaataaac gaatattatt agcatctcct catatgagtg atgaaggata
     6841 tgaacaagag tatgtaaaag aagcatttga tacaaattgg gtggctcctt tgggtatgaa
     6901 tgtcaatggt tttgaagagg aattagcaga atatgtcggt gctagtcatg cagctgcact
     6961 atcttctgga acagcagcta tacatctagc tttaaaagcg gctggggtcg gtaaagatga
     7021 tatagtgatt tgtcagtctt tgacgttctc agctactgcc aatccaatca tctatcaagg
     7081 agctattcca gtatttgttg atagtgatta cgaaacttgg aatatgaacc ctgaacattt
     7141 gcgaacagcg ctagaaaaat atcctaacgc aaaagctgta ttagtagtgc atttgtacgg
     7201 tttatcagct gaactagatg aaattactaa aatatgcaaa gaacataatg ttgttttaat
     7261 tgaagatgct gcagaatcat taggaacaac atataaagga aaatatactg gtacattcgg
     7321 cgactatgga atttattctt ttaatggaaa taagataatc acgacttcag gtggcggaat
     7381 gcttgtgtct aatgacaaag aaaaggtgga aaaagtgagg ttttgggcta cgcaatctcg
     7441 tgagaaggca cgccattatc aacacaatga aattggctat aattatcgaa tgagcaatat
     7501 cgttgcaggt atcggtcgag gacaattgaa agttttaaat gaaagaattg agaaaaaacg
     7561 atatattcat agtttttata aaaaagaatt gtctaaattg tcaaatatac aattcatgcc
     7621 agaaaataat tggaatgaac caaattattg gttaagtgtc ataacagtaa agggaataaa
     7681 tacgaatcat cttattgatg agttagataa aaataatata gaagcccgcc cgatatggaa
     7741 accaatgcat ttacagccag ttttcgataa gtatgagtat attggtacag atgttgctca
     7801 gacattgttt gaaaaaggta tctgtttacc ttcagattct aaaatgaccg atgatgattt
     7861 gtatcgagta actaatacaa ttaagcgagt gatgcaaaat gtctaataga ggtatctacg
     7921 aaaagttctt taaacgtttg atagatattc ttttttcaat tttaggttta ataatgttat
     7981 ggccactgat gacgtttgtt gcaatcctag ttcgaataaa gcttggatcg ccagtgatct
     8041 ttaaacaaca acgacctgga aaacatgaaa agatttttct gatgtataaa tttcgaacaa
     8101 tgacagatca aagagatgaa gaaggtaatt tattatcaga cgatatcaga ttgactcaat
     8161 ttggtaaaat attgcgttcg actagtcttg atgaactacc agaattatgg aatattttaa
     8221 aaggagatat gagtattatt ggaccgcgtc ctctgttagt tgagtatctt tcactataca
     8281 gtaatgaaca acgaaaaaga cattttgtgc gcccaggttt aaccggatta gctcaagtca
     8341 gtggtcgtaa tgcaattagt tggaaagaaa aatttgaaaa agatattgaa tatgtggaga
     8401 acatttcaat gatgaaagac ataaaaatat tttttttaac gctaaagaaa gttattgtcc
     8461 gtgatggaat aacatcagta gcaagtggga ctactgagaa atttgagggt gcgaagatag
     8521 atgagtgaga ataaaaaaaa gtggctgctt attcatcctt ttgattatat cagtcgaacg
     8581 aatatattag aggaaattct gacggaaaac agtatcgaat attttaatgt tgtatctgat
     8641 tactcacata taagaaaaca atttctatcc agtaaagaaa ggcatatgat taaaaatcgt
     8701 catgtgataa aaacgatcgg atataaaaaa aatatatccg taatgcgaat tttatctcat
     8761 atatggtttt caataaaaac attattattt attcgaaaag aaaagcctga aattatttat
     8821 tgtagaatac cacctaatac cttagttttt tttattgcta agtttagtag atgcaatcga
     8881 gttatttttg atgtgtatga tttatggcca gagtcaatgc cgggaatagg gaacaaatat
     8941 aaatttttta ctgatttatg gaaaaagcta cgagatcaat atctcagtaa ttctagtcta
     9001 gttataacag aatgcgatta tttcaaaaaa ttcataaaaa tttccggaga atcaaaaaat
     9061 atgaccattt atccatgttc tttcaatgta gatgaccaaa atgattatgg agagaaaata
     9121 gtcaataaaa gcttagagtc tacgttgaac cttgcttatt taggtacgat taataatatc
     9181 gtagatttag aagcgattaa tgaattattg aaaacatgca aaagaaaatt tggaaacaaa
     9241 ataaatcttt actttattgg aaaaggtgaa agcaaaacta aattagctga tatttgtgaa
     9301 aaaaatcata ttgaatttgt tgactgcggt gttatatatg acaatgcaga aaaaaaagtt
     9361 atactcgata aatgtcattt tggactaaat ttatttaagt caaataccat aattggttta
     9421 agtctgaaat cgattgagta ttttaggttt ggattaccta taatttcaaa tttatcagga
     9481 gatacgaaag aaatagttaa aagatataat gtcggaatca attataatag taccgttgat
     9541 gaaaatgaga tgttgaaatt ttatcagtgt aataaaaaca taaatgaaaa tgttagaaaa
     9601 atttacgcca ctatattctc tgagcaagta ttgaaaatga aatggaatga agtgtacaag
     9661 gtgatgaacg atgaaaaata aaattgggat aattattgtt acctacaatc cagataataa
     9721 cataaagtta ttagtggata cgttaataca agagaaacag aacaaactta ttatcgtgga
     9781 taacggctca gataatatta actcttttca atttgaaatg atggaaaact taattttaaa
     9841 aaaaatgaat gagaacattg ggcttgcgac agctcaaaac ataggtattg aaattgccaa
     9901 aaaagagaaa tgtgattata tccttttact agatcaagat accatcatac aaaagaatct
     9961 ttctttaaaa ttattagaag agtttaccaa tattgaagcg aaagataagg acattgggat
    10021 tattgttccc aattactttg agaatagcaa tgatgaatct gtctctttta cctgcaaaaa
    10081 tgagagaggg agatacgtaa aaaaacactt tgcttcaact agtggttcca tgaaaatagc
    10141 atttgcaaat tcctctggaa tgtttttcaa aagtgaatta attgacgatg ttggaaatat
    10201 gaaggatgaa ttttttattg atcagattga tgttgaattt tgtaaaaggg ttgcaaataa
    10261 tggctataac atttatgtaa caaatcaagt ctatttttat catcacgttg gagatggaga
    10321 aaatttaaaa ataggatcaa aaaaaattaa aatagacaat cataattcaa cacgaaagta
    10381 ttttattgca agaaattcaa cattggtgaa aagaatgtat aaaagtgata aggaattttc
    10441 ccgccttatg aataaaagat tattgaaatt ttttattgca atagcttttg agactgataa
    10501 gctcagtaga tttagagcgg ctataagggg ggttcgagat ggatacagag gcgtcattgg
    10561 agattaatca agaaaagagt gtttcacaaa aaaaatggag tacatcgaac aagattttgt
    10621 ctggggtgct acttttgata atgtggatca tcatggcatt taacacacaa aacgctgatt
    10681 attatgcata ttctgtcttg tataatggga tagcacaatt tggtatggac tttagttcag
    10741 atagtattga accgggattc agagctgtta tgtacgtatg tacctttatt tccagtcagt
    10801 atcagatttt tttattgatt atttctactg tcttactctc agctttattg tatgtcatca
    10861 gattattcac aactaatgtt tttttagttt tgttcttgtt ttttattttt ggttttactt
    10921 cacaagcaat acaagtacgt atgtttagcg cattgatatt catattacta gctttttatc
    10981 atctaactaa attgcgaggc agcaaagcta tatttaagta tatttttttt atcttagtgg
    11041 ctggactgtt ccataatctt gcatattttt atctcccttt ttttattgta aaaattaata
    11101 aaaagattac tggatatgtt acaatcctta gcttgctcct attctctatt ggacaaagtt
    11161 atatcagttc aatatttata aatacgaagt atgatgcata ttttgataga aacagtacat
    11221 cgatttctca cgtagtgatg tttgctctct ttttttcagc aaatcttttt ttagttcata
    11281 aattatcaaa aatgaatatt tacgaaaaaa aagttatcag cttaagctca tatatatttt
    11341 ctatcaatat gttagcttct actagtatag ttttcttact tataagtaat aattttatta
    11401 gaattattca aaatatattt gtattaaatt atattattta tgccagtgta ttaatgagta
    11461 attataagag aacaaaagta aagaattata tctacttgct tgcgtatttc ttttttatca
    11521 ttgtgtgttt gatattcatc aatataagag gaactgcttt tgtaaatgga atcctaccat
    11581 tatttagaga aaatattatt tttaaggctg tttactattt ttttgagtaa gaataaatag
    11641 cacttaaaaa tagagatgag ggatttatgg gaagacaaga attatatagt ttagctatca
    11701 atgaattgga aaaaagaaat cttgaaatac atgaattaaa tcaaaaaaat aattctattt
    11761 taggcatgaa aatacgaaat aaaattaatc aacttaacca aatgaaaaaa cgagataatc
    11821 tgttgccagc aaaatatacc cttaaatcga tgaccggtaa ggggcctatt aaagactatt
    11881 caaacagaga gaatcgcagt gatgaactaa gaaaaaaaga gttaactaat caaggaacac
    11941 ctaaaaggat tgctgtgtat agttgtatca ctggtaatta tgattctatt aaagaaccgg
    12001 atttttctca tccattggta gattactatt tgtatacaga taatgtgatt acaagtgagc
    12061 attggaatat tagagagata cctaaatata tatcggaatt aaaaaataat attttgatca
    12121 atagatatat taaatttcat cctaatgagc tatttactga ctacgatttt actttgtatg
    12181 ttgatggtaa tgtaacaata acttctgata ttaccggatt actgaacagt tcttccaaac
    12241 ttgggttaag tatgcataaa catagtgaaa gaaaatcttt atattctgag gcagatgcat
    12301 taattgcagt tggcaaaggt aataaggaaa gtattataga gcaaattagt aagtacagag
    12361 aagaaggatt tcctgataat tttggattgg ctgaagccac ggttatttta gttgatttaa
    12421 ataattcaat cgctgagaat cttctagatt tgtggtggag agagtatttg aactcagaaa
    12481 gtgggcgaga tcagctcgca tttccttacg tgttgtggaa gtccggacat gcaatgaagg
    12541 aagtgactac cttaggcaat aatgtatata aaagcgctaa attaagaatc ataagtcatt
    12601 attagcccta aatgatttct aaactattct ttcaaatcct agtaaatatt tggagataga
    12661 agatttcctt tttaaagtca taaaaaatta agttatattt tttgagtgaa tggagaagac
    12721 gcatagtatc ataaacttcg ttatatctta aattatgggg tatgcctaaa taataaatgt
    12781 aatacgagta taaagaagaa agcaattttg ggtttaaccc tattggttcc tttcagaaaa
    12841 aggtggatga ataaaaatga aaatattaat aacaggtgga gcaggattta ttggttttca
    12901 tttgtctaga aaattactca aggaaactga aaatcaaatt gtagcgattg ataatctcaa
    12961 tgattattat gatgtccaat taaaagaaga tcgattaacg atactgtacg attataataa
    13021 ttttaaattt atcagaatgg atctattaga gaaagaaaaa ttagattctc tatttgaaag
    13081 tgaaggtttt gacatagtaa ttcatttagc tgctcaagca ggtgttagat attctattga
    13141 aaacccttat gcttatattg attctaatat tatcggtttt cttaatattc ttgaagcctg
    13201 ccgaaacttc cctgtaaagc atctattata tgcatcatct agttcagtat atggtgggaa
    13261 cacgaaagta ccgttttcaa cagaagattc ggtggatcat ccagttagcc tttatgcggc
    13321 taccaaaaaa tcgaatgaat taatggctca tacttatagc cacctttata aaataccaac
    13381 aactgggctg agatttttca ctgtctatgg tccatttggg cgaccagata tggcctattt
    13441 ctcttttacc aagaatattt tatctggaaa agaaattaag atttttaacc atggaaagat
    13501 ggaaagagat ttcacttaca tcgatgatat cgtagaggga atcactagat taatcaataa
    13561 agtacctgaa cccaactccg actggacaga catcgatgat ttgagtagtg aaagttgggc
    13621 accatataag atatacaata ttggaaataa taagccggtt gcattgttgg agtttgttga
    13681 aacaattgaa aagtatttag gtgttaaagc gaacaagatt tttttggaca tgcagccggg
    13741 tgatgtcgtt agaacttatg ccgatattga agatttagag gaaaaaatca actttaaacc
    13801 gaatataacc ttggatgagg gcattaaaaa attcgttgaa tggtatacag tgtattattt
    13861 gagttaattt tgaaaggaat attgaagatg ggtaattcat acgatttttt tagctttgtt
    13921 aggtatttta ggaaattcta ctttcaaacg tgacgaactt tctcgccgaa tagatatttt
    13981 ctataactaa aagaatttct ataaaacaaa attgtgtatc aagatgtgtg aactggattc
    14041 tgactgccaa tattgattga tgagattgaa gaaatatata gttgatcatg gatatcttta
    14101 tttgaaaaga tcttagctat tcacatattc aaaaaaattt ttactacgag gactcacacg
    14161 atacagagga atttaggtag ttctaattaa accagaaagt catctataga tgtgagttga
    14221 gggaggtcat acggttggat agtagcagta ataaatcaca ttcaatagct aaaaaagctt
    14281 ttatttacgc aataggaaca ttatcatcaa aaatgatatc tgtcattata atccctatct
    14341 atgcagtata tatttctgtt gccgatttag gaagctttga ttatcaaaat acaattgctc
    14401 aatttctttc tccagttatt tttggagcaa tatgggaagc aattttacgc atgggattag
    14461 gcaaagaaaa aaatattcag aaaaaagtag tgacgaacag tatttgtttt gttatagcaa
    14521 atacagtgat attagccatt tttttaccaa taatatatag aatgattttc ccgaatttag
    14581 agtattatca attgtttgtt tttcaattaa ttttcactcc tatcctaagt gtttttcaat
    14641 atattgtaag agcactaggt gaatcaaaga tttttgtgat gagtggtata atcgctgcgg
    14701 gtatcaacct gttactcctt actatttttg ttgttacaat gggtatgggg atacttggtc
    14761 tcttactctc tatcataatt tctaatgtta taaatttgat ttttttgatt ttcagggggc
    14821 agatctttca atatttcgca acgagttctt ttgatttttt atttttaaag tccatgctga
    14881 aatactctat cccactcact ttgaatctca cttttggatg gctaatagtt ggtttttcaa
    14941 gattttatat tggaacgtac atcggagtaa cagaaaatgg tattttttct tttgcgatga
    15001 aattttcatc tataatttta actcttacaa gtattgttag tatgtctatg atagaggatg
    15061 cggttatcac gtctgagaat gatgatttca tcgaaagatt cgttgagaaa aatacctata
    15121 tttttaaaat gtacattagg ttagcgactc tttctattcc tatcatttgc tgtgcatact
    15181 cttttataac caaccctgat tataaaaaat cattagttct agttccaata ttagttatta
    15241 tggcatgttt cagtgggatg acaacaaata tcggaaatat atttgcagta tacaataaaa
    15301 caaatcagtt attaatgatt tcagtagtgt caggtctatt gagtgttgtt ggcacctatt
    15361 tattaggcta ttcttttgga gtaattggtg tctcatttgc tactttgctc ggtactttag
    15421 tattatttgg acttagatat tattttggaa ggaaaattgc ttattatgca tttgattgga
    15481 agcgaatatc gctactttta gttctctatt ccgcaatcgc ttttgtatgc attcatagag
    15541 gcttctattt taatcttttc ttaacgatta tttttagtgc tattttcgtt tttacgtata
    15601 aacaattaat tataacgtat ctaaaaaaat ttttcagaat aaaaatggct aaaaggtgag
    15661 ggtatacgaa gtaagaatag aatctttcga agaataaaga gaaaactatt tggaaaaaat
    15721 ctgcgagaat gtattaatga aggtttgata ttcggaaaaa aacatagttt agtagaggtt
    15781 cctgaatttg ggagcgagcc atatttaatt gaaatagggg ataatttcag atgttcattt
    15841 ggtgttaaat ttataacaca cgatggtgga gtctgggtct taaggaagat gagttctgtg
    15901 agcgagtcaa cttcaaaaat aggtaggatc aaattgggaa ataatatttt tattggtatt
    15961 tactctattg ttttcccagg agtttcaatt ggcgataatg taattattag agcgaacact
    16021 ttagtaaata aagatatccc aaataatagt gttgtagcgg gtagttcatg taaagtgatt
    16081 tcaactatag aggagcatta tgagaaaaat aaaaatgaat ttattaaata tcgaacctcg
    16141 atgattttga agcaaaaaaa ttttttctat taaataatat tgattgaata aggaatatgt
    16201 tcaaaaggat aacagctaaa atactataaa atgaaatttg attggagcgt atggaagaat
    16261 atgaaaattt cagttttcgg attaggctat gttggtctag caaattcact attattagca
    16321 caaaaagagc aggtcaaagc ttatgatatc gtggaagaaa aaatcacgat gttacaaaat
    16381 ggcatctcac caatagaaga taaagaagtt catgaatttt taaaacggga tgacttaaat
    16441 gtcgaattta cgtcaaattt tactgatgca gtaaacttcg gtgattactt gattattgca
    16501 actccaacag attatgacga aaagaaaaac tattttaata catcaacagt agaagatgta
    16561 attgagaagg ctttagcaat tcgtccagat tcaacattca ttattaagtc gacagttcca
    16621 gttggctatg caaatgaaat taaagcgaaa tttggtacag aaaacatcat tttttcacct
    16681 gaatttttgc gtgaaggaaa agctttgtac gacaacttat atccatcaag aattatagtt
    16741 ggagaatgtt ctgctcgtgg tcgcgagatt gcaactatgt ttcaaagaaa cgcatttaac
    16801 aaagatgcat cagttctttt gactcactca actgaagcag aagctattaa attattctcg
    16861 aatacatatt tggcattgcg agtggcgtat ttcaatgaat tagatacgta tgcagaattt
    16921 cgtgggcttg atagtaagca aattattgaa ggtgtcggtt tggatccacg gatcggttcg
    16981 cattataata atccttcttt tggctatggt ggatactgtt tacccaaaga cacaaaacaa
    17041 ttaagagcca actatgaaaa agtaccaagc aatatcatca gtgcaattgt tgatgcaaat
    17101 acaacaagga aagattttat tgctggacag attttggcga aaaaaccaaa tgttgttgga
    17161 atctatcgtt taacgatgaa ggccggttca gataattttc gatattcgtc gatccaagga
    17221 attatgaaac ggattaaagc caaaggaatt gaagtggttg tttacgagcc tacattggat
    17281 gaagatgaat tttatcattc acaagtattg aaagatatta atgaatttaa aaatatttct
    17341 gatgtgatcg tatctaatcg gtttgaagat attttaaagg atgttgaaga aaaagtttat
    17401 accagagata tctttggtga taactaaata ataatgaacg ggtacatgga ggaaaatatt
    17461 ttagatggtg actcgaaata aatgtttgtt ctcattgcat ctgtatctat accgataagt
    17521 ttaaaaattc cgattaggaa aatgaagttg gctatccttg cgaggaattt attgctggat
    17581 tattttatga actaaagcaa gacatcatca gaaaaaaatc caaaatttga caatgggatt
    17641 gatcgtttac cagctgtaag cgaagtatct gacacgaaaa tggaaaatgc aattactgtt
    17701 attaccaata gctttttttc atcgatcaag tatttgaaaa cattatttgt ttgccaagct
    17761 gatcaatgtt ctttcagact tggaagaaca ttgatcagct cacaagtaat gattgaagtt
    17821 taggttatgg gataaaggat aagtgaaata aaagtcgtgt ctgcatcttt gttagtacca
    17881 gtaaattttt ctactacggc gctagtcacg acagtccagg catctgaaat tgaagatgca
    17941 aatagagttt ctgctagttt aggtattgat gttactgtat tagcattaat cttagagcca
    18001 gtagcatcca ataggtgtag ctatagttct aatagtataa aacaatgcag aggatatgat
    18061 tgctttatgt ctcggtgtgt caggtcaaaa tagagttcca tatggggcga tttctcaatg
    18121 aataatctta tagatataat cttgtttgct atttcgttat cacttttact tttcttcgat
    18181 tatttcaaaa ataaaaatcc aaaatatttt aagttcttac tatttatcat tactgtacta
    18241 gctgttttat cgatgacaga ttttatttaa cttatctcaa aaagtaggga accatatcat
    18301 ttttcagtaa catgcgtatt atttgtataa ataatacaaa atgaaaaatt atgattattc
    18361 tattgtcttt ttagaaagcg ttttctgtga gcttttaatc taaaaggagg gatataaaat
    18421 aaagaaaacc tattttatgc tatttttttg ttgtttctgt gtaggaatcc tgtggtatat
    18481 aggtggtaat gaaaagaaga ttcatggagt tgtgacaaat atttctgcgg atgagttcgt
    18541 atttgaagat gaaaaacaga acgaatatag tattcaaaaa agaagctctt tgtcaaatga
    18601 tgttggtaaa gagaaatctt agaataattt ggacagaaga ggctgggaca gaagtgttta
    18661 actccgagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcggc
    18721 ttatttccga aggagttgct tctgctcccg ccgtttactc gagagtctgt agcaaaactg
    18781 atttttagat ttatcccaca ttcttctgtt tatttggtta aaaatgtttt gccatgacga
    18841 atcatgatcc gtagtttgaa attgtaaaag ctgcgaaagc catacgtatt gcgttttaat
    18901 actttgatgt ggttgtttaa acattccaat ggtctgtttg aatagctgca gttaagcgca
    18961 ttttgaatct gtggcagaaa atttttaaac gttcgtaaag tggttgtata ggtttcaggc
    19021 agatcgctaa tgtctctagt taaacagtcc tcaaatagtg acgaatctct tttctttcac
    19081 tcaatgattc aagaaggttc ttttaatata ttggaagatg tggatattct tctggtgtgt
    19141 actgaagagt ccctttgtaa atcaagcaaa tttgtccttt gtatttttct ttagttaaac
    19201 aattttcctc gaaatggata ttgatattta ggtctagaat atttagggta tctttgataa
    19261 gcagagtatg agacatcttg aatcctccta tgaatttgtt gggcaaatta attctaaagg
    19321 atttcagggt gtcttttcta tattttttta tacaaaaaag atgttgataa agttcacaaa
    19381 catcgaaaag tatagagccc gaattttacg aatatttttt gtaatataaa agtatagata
    19441 ctaaattctt attcgttaaa cttacgccat aaggaaacta gaaaaagaca gaattatcat
    19501 aaaaaatttt tgttattttt aaaaatgtac aacaaaattt cgactatcag accggcatga
    19561 gagaaaagtt atctaaaaaa taaaaaatcc tactttattt agagaaagcc atttttattt
    19621 acatagtagt ctattaggcc catttgcttg tcgatagtag aaatagccac agatgtataa
    19681 agaatatttt ttctttactt ctctagacca tttagctgaa tctttgccaa gattcagctt
    19741 ttttttgttt tttatttgtc aactggtatt tttgaggtat cttttgatac cagttctaaa
    19801 ttaccttgaa accttgttat aatagctttt ttacgccaaa aaccctttga caaatgagga
    19861 atatgtgggt attcttgtgt tgtggttata accagtaaag aagaggtatt tttatggaga
    19921 gctcaagtaa aggaaaatca ttatattatc aactagttga cgtattgaaa gaacgtatcg
    19981 aaacacagat gacacctcat gacaaattgt tatctgaaag agaactttgt cagcattatg
    20041 gagtgagtcg gacaacggtt cgccttgctt tgcaagaatt agagacgcaa gggtatattt
    20101 atcgccgaca cggaaaagga acatatgtgt ctgatctatc agataagtcg tctgatctgg
    20161 cgggagcgta tagttttaca gaagagatga gggaattggg acgggtgcct aatacagtca
    20221 tcttggaatt caagcaaatg gaagcaaaca aaaaggttgc caagaatcta aacatcaatt
    20281 taggcgaaac agttttcaaa ttgaaaagac ttcgtttagc ggatgacgaa ccattgatgg
    20341 tggaacgttc ttatttacct gcaaaactat ttattggtct tgatctgcca ttacttgaaa
    20401 gtaagccatt gtatgatgtg ttttctcagg attatgaaca gcaagtccaa ttagcagatg
    20461 aagaatttta tgcaagtatc gttcaaaaga aagatgctga tccgcttcgt atcgctgatg
    20521 gtgccccggt attatatcta agccgaacga cctataatgt gaaaaatgaa attattgaat
    20581 atacggatag tgtagcgaga gcagaccagt ttcattataa gatccgccac gtccgaaaat
    20641 gattgagagg gaaaacaaca tgtttgaact atcgaaggat aaattaaaag agttgggtgc
    20701 ggaaataact acgcgagaaa tcaaacagca accgcaatta tggaaagaaa cagctgagat
    20761 attagcacag aatcaagaaa aattagcatt gttttttgac cagataaaaa aagcagcaaa
    20821 tggcaaaaga gttcgagtga tttttactgg tgcaggcaca tcacaatatg tcggggatac
    20881 agcagtacct tatttaaatg aatacggtga taacgatcac tttatttttc atagctttgg
    20941 tacgacagat atcgtgtctt caccaaaaga atttctattt ccggatgaac cgacattact
    21001 ggtgtcattt gcacgtagtg gaaatagtcc agaaagtgtg gcaacagtca agattgccaa
    21061 tgaacaaatc aagacattgt ttcatttgac gattacttgt gctaaagaag gtcagttagc
    21121 aaagaatgca gcgacagatg aacgaaatta tcttttactg atgccagaaa aatcaaatga
    21181 tgcaggattt gccatgacag gaagttttac gtgcatgttg ttgagtgcgt tatgggtatt
    21241 cgaccaaact gccgttgaga aaaaagaaag ttatcttcaa caattgatca gaactggaga
    21301 agatctaatt tcacgagatc aagaaatcca aaaattattg cctaagtcat ttgaacgtat
    21361 cgcctatctt ggttcaggaa gtttggctgg tgcaacgaga gaagcacaat tgaaaatatt
    21421 ggaattgaca gccggtaaag tggcaacgat ctttgattct tctatgggct tccgccatgg
    21481 accaaaatcg tttttaaata gcgaaacact tgttattggt tttgttaata accaaccgta
    21541 tacaagaaat tatgacttag atattctaga agaaataaaa gatgaccaaa ttaccacgag
    21601 tgtcatcgcg atcgcccaac caggaaacat cgatttcaca ggtacaagtc ttttgttaga
    21661 agcttcgatc agcctacctg atgcgtatct tgccttacct atgatcatga cagcccaaat
    21721 cattgcgctc gatacgtcga taaaagtagc gaataaacca gatacaccat cgcctacagg
    21781 tacagtcaat cgagtggtca aaggtgtcat tatccatgaa tatgtgaaca aaggggaaac
    21841 gaaggatgaa tgaagaaaaa attgcagcta tgaagagttt atcaacaaaa gaaggaatca
    21901 tcagtgcatt ggcaattgat caacgcggtg ctttgaaaaa gatgatcaaa gcgttaggaa
    21961 aagaaccaac ggcgcaacag atcactgaat tcaaagaatt agtatcagca gaattaacgc
    22021 cttatgcttc tgctatttta ctagatccag agtatggatt gccagctgct aaagccagac
    22081 atcaggaagc aggactactt ttagcctatg aaaaaacggg ttatgatgcg acaactccag
    22141 gacgtctgcc tgatttatta gcggattggt ctacgcttag attaaaggaa caaacagccg
    22201 atgcgatcaa gtttttgctt tattatgatg tagatgaaga cccagagatc aatcatttga
    22261 aacatgtatt tatcgaacgt ttaggcagtg aatgtgcagg tgaaaagcgt ccgttttatc
    22321 tagaattggt ttcgtatgat gccaaaacca atgacagcac ttcattagaa tatgcgaaga
    22381 tcaaaccgca taaagtgatc gagatgatga aagaattttc tgatcctaaa tataaagtgg
    22441 atgtattgaa agtagaagtg ccagtcaata tgaactatgt agaaggttat tctgatggtg
    22501 aaattgcgta tagtaaaaaa gaagcagcgg cattcttcaa ggaacaaagt gatgcaacac
    22561 cgttaccatt tatcttcttg agtgcagggg tttcgactgc attgttccaa caaacattga
    22621 tctttgctca tgaatctggt tcaacattca acggtgtgtt atgcggacgt gcgacttgga
    22681 aagatggagt caaagcatat gttgaaaatg gtagagaagg tgcgattgag tggttgagaa
    22741 cacaagggcg acaaaatatc gaatcgttga ataaagtgat aaatgaaaac gccacccctt
    22801 ggtttgacaa agtaaaagaa gattcggtga cagcaaaatg atcacgacag ttaccatgaa
    22861 tccttcaatt gacatcgctt acacgttgga tgaattacgg atcgaccaag tcaatcgggt
    22921 caaaacggtc aacaaaacgg ctggaggaaa aggcttgaat gttgctcgtg tccttcattt
    22981 attagcagat gatgttttag ctactggatt agtcggcggg cagcatggag catatatcaa
    23041 agaacaactc gatcaatcag ggataacgca tcaattttta cctatcaaag gggaaacgag
    23101 agattcgatt gctattttac atggagtgaa tcaaacagaa ctattggaaa gcggtcctgt
    23161 tgtgacggaa gaagagcaac ataaatttac cgagcatttt gcacgactaa tggaaacaac
    23221 tgatatcttt acgatttcgg gtagtctgcc tcaaggattt tctaaaagtt attatgcaga
    23281 gttacttgcc ctaacgaata aaaaaggaac acgagtattg ttagatactt ctggtcaaac
    23341 cttgcttgaa gtcttggaag gcaaggaaaa accatttttg atcaaaccta atttggaaga
    23401 attaggagag ttggtccacc aaacaatttc ttcggctact tcgttagatg aaatccaatc
    23461 attattggat catccgcttt ttgaaggaat caattggatc gttctttcgc taggcgcaaa
    23521 aggagcaatc gctaaagtag ggagacactt ctatcaggtt acgattccta aaatcgcggt
    23581 ggtgaatcct gtggggtcag gagattcgac tattgcaggc ttagcacatg ctctatcaaa
    23641 gagctatacg attgaagaaa cacttgcgtt tgcgatggcc tgtgggatgg cgaatgcaca
    23701 acaagaaaaa acaggatgga tcgatcctgt gaaagtcgat tattttaggg gggaaatcaa
    23761 cgtcagaaag attcgttgat tataaaatta gaaagcggtt tctaaaaacg aggaggaaat
    23821 ttgcttgact agatttgaaa taaaagaaga gtttttgtta gacggtcaac ctttcaaaat
    23881 tttatctggt gccatccatt atttccgagt gcatccgacg aattgggagc ggtcattgca
    23941 caatttgaaa gcgcttggat ttaatactgt ggaaacctat gtgccttgga atctccatga
    24001 aaaagaagaa ggaacctatg atttttcgaa aatattggat ctttcccatt ttctagagtt
    24061 agcgcaatcc cttggattat acgcaatcgt acgtccagcc ccttatattt gtgcagaatg
    24121 ggaatttggt ggcttgcctg cttggctatt gacaaaggag tgccgtattc gttcagcgga
    24181 tcctcgcttc atgaagtatg tcaaacggta ttaccaaacg ctactaccta aaatcgttcc
    24241 tcatctagtc actcatggtg gcaacgtctt aatggtgcag gtagaaaatg agtacggttc
    24301 gtatggtgaa gagaaagcat acctgcgcca agtaaaaaaa tacttggagg aaggtggaat
    24361 cgatgtaccg ctattcactt ctgatggacc ttggcaagcc acattaagag caggcatttt
    24421 gattgacgat gatgttttta cgacagggaa ttttggctca aaagcaaaag aaaattttgc
    24481 agatatggcc cagttttttg aagcacatgg taaaaaatgg ccgttgatgt gtatggaatt
    24541 ttgggatggc tggtttaatc gttggaaaga gccgatcatt aaaagagatc cggaagaatt
    24601 agcggatgca gtcaaagaag cattacagat cggcagtatc aatctctata tgttccatgg
    24661 cggaaccaat tttgggttta tgaatggctg ttcagccaga ggcgcgattg atttgccgca
    24721 agtcacttcc tatgattacg atgcaccatt ggatgagcag ggaaatccaa cggaaaaata
    24781 ttttgctctg aaacgaatga tggcagaaaa ttttccagaa atgcagcaaa tggaaccatg
    24841 gataaaagaa acaatcgtca aaaaaggaat caacttgaaa gaaaaagtca gctttttcgc
    24901 aacactggat accatcagca agccgatcca ctcgacctat cctcaaacaa tggaagaatt
    24961 gggtcagaat actgggtacc tattgtatcg aacaacgatc aaaaaagatg cggtcaatga
    25021 aaaattacgg atcatcgacg gacgtgatcg ggtccatgtt tttctcgatg aagaaaagca
    25081 agcgattcaa taccaaacag agatcggtga gaccattcca ttgacacttg agaaagaaga
    25141 ccatcaaatt gatctattat ttgaaaatat gggacgggtc aactatggac acaagctgct
    25201 agctgatacg caaagaaaag gaatacgtac aggagtgatg agcgatcttc attttatcac
    25261 agaatggaca cagtattgtt tacctttaga gactacagaa cacatcgatt tttcaaaaaa
    25321 atgggaagca ggtcaaccag cattttaccg ctttgaagcg gaactgtcag aaatcggtga
    25381 tacttatttg gaccttagtg agtttgggaa agggatcgtt tgggtgaatg gcacgaatat
    25441 tggtcgtttt tgggaagtag gaccaattct ttcactgttt gttcccgagg gtttatttag
    25501 aaaaggacaa aatgaaatcg ttatctttga aacagaaggt agattcagtg aagttataga
    25561 ttttgtcaaa gaaccaatag aaaaaagtta gaaaaggaga atggatgatg gcaattattg
    25621 gagtaagaat tgatggacgt ttgatacatg gacaagtggc aaatctttgg acgacaaagt
    25681 tgaatattgg tcggatcatg gtcgtagacg atgaggtagc aacaaatgcg atcgaaaaaa
    25741 gtgggttaaa gttggcaacg ccagctggag tcaaattaag tattttacct attgaaaaag
    25801 cagccgcaaa tattttagca ggtaaatatg attctcaacg agtgctgatc gtagcgaaaa
    25861 aaccagatcg tatcttagcg ctagtagaaa aaggtgtacc gatcaaagaa ttaaatgtcg
    25921 gcaatatgtc acaaacagat gaaacacgtt ccatcacaaa atcaatcaat gtcgtagatg
    25981 cggatgtaga ggtcttcaag aaattagatc agcttggcgt tcatttgatc gctcaaatgg
    26041 tgccaaatga caaagcagaa gactttatga gtcttttaat caaataggag gaatgattca
    26101 tggatattca atggtggcag atattattat taacattata tgcagggtat caaattttag
    26161 atgaattaca gatttattct tcaatgagcg ccccagtttt tgcaggatta tatgctgggt
    26221 tagtaatggg tgatttaaaa gcaggtttaa tcattggtgg aagtatgcag ttgactgttt
    26281 taggggtagg gacatttggt ggtgcatcaa aaatcgatgc caactctgga acgatcttag
    26341 caacagcttt ctccatttct ctagggatga acccagaaca agcgattgcg gccatcgcgg
    26401 ttcctgtagc aagtttgatg atccaattag atattttggc acgttttgcg aatacgtatt
    26461 ttgcacatcg gatcgatcaa catgtggaaa caatgaatta caaagcaatt gaacggaact
    26521 tcttgatggg ggcattacct tgggcacttt ccagaatggt tcctgtattc ttggccctag
    26581 cattcggtgg tggcttagtc caaaatgttg tggctgtttt aaacggcgac ctaaaatggc
    26641 taggagatgg cttatctgtt gcaggggcag tcttaccagc agttgggttt gcaattttac
    26701 tacgctacct accagtaaag aaacacattt cttatctgat tttaggattt acgatcacag
    26761 cgttattagc aacagtgttc tcgaatgtcc aattgttagg aacaagtgtg gctagcgtgg
    26821 tcaaagattt cccaggaaca ttcaattcat taccgatgct tgctgtggca ttgattggtc
    26881 tagcactggc aacgatcagt tataagaatg atcaaaagaa tgaccaacat ccagtagtaa
    26941 gtcagcaatc acaatctgat gagggggaaa tcgaagatga cgaaatctaa ttataaattg
    27001 accaaagaag attttaagca gatcaatcgt agaagtttgt ttactttcca attaggttgg
    27061 aactatgaaa gaatgcaagg ttcaggttat ctgtatacta tcttgccaca acttagaaaa
    27121 atctatggtg atgatacacc agaattaaaa gaaatgatga aaacacatac gcaatttttt
    27181 aatacgtcta atttctttaa cacgatcatt actgggatcg atcttgctat cgaagaaaaa
    27241 gaaggagtct cttcaaagga aactgtttct gggatgaaag caggattgat gggttccttc
    27301 gctgcaattg gtgattctgt atttgcggca ttgatcccga ccatatttgg agctttagcc
    27361 gcaaatatgg caacagatgg taatccagta ggtatcctta tctggatcct tgctcaggtc
    27421 gctgtcatgg tctttcgttg gaaacaacta gaatttgcct acaaagaagg gatttctcta
    27481 gttaccacga tgcaatcacg cttgactgca ttgacagatg cggctacttt actaggagtt
    27541 ttcatggttg gagcgttagt agcaaccatg atcaatgtta aattctcatg ggcaccaacg
    27601 attggggatg taacgttgaa tatgcaaaac tatatcgata tgatcttacc taaactttta
    27661 ccagctggaa tcgttggtgg cgtttattgg atgttaggta agaaaaatat gacacctact
    27721 cgagcaatat tcatcgtatt gatcgtatgt gtagcattat ctgcattagg tgtgatttcg
    27781 aaataaggga gaattggaaa gatgagtaga caactagtct tgattagtca tggtaaattt
    27841 tgtgaagaac taaaaaacag tacagaaatg atcatgggac cacaagaaaa tatctatacg
    27901 atccctttat tgcctgaaga tggcgcggac acgtataaag aaagattcct ctccgttaca
    27961 gcaccattga aagattacgt agtgctttgc gatctgttgg gtgggacacc tgcaaatacc
    28021 gtttccaagc taatcatgga aggtgaaacg atcgaactat atgctggcat gaatttgccg
    28081 atggtcatcg aattcatcaa ttcgcaaatg attggagcag aaccagattt tattggttcg
    28141 ggtcgtgaga gtatcgtttc agtgaaccaa gtgattgctg gcatgggaga cgatgaagaa
    28201 gaataatcaa gcacaacttc ttgactggtg atacaactgg atataaatac ggatgtataa
    28261 agaggccgtg tataaaaaag ccaacgtacc gcgaaatata gttttctata tttcacagta
    28321 cgttggcttt tatcgtcata aaatcagaaa tgatctgggt cgatcgaaga agctagttac
    28381 aaataactat ttacgctttt gctgtttacc agcattccga ccattcttcg cattgttttt
    28441 tggtgcttgt acttgttttt ttgtttgctt cgcaggttca gcaggtgtga catctttacg
    28501 aggcgtcacc acttgttttg gcggattttg ttttaattct tccgcaactt gcgcttttaa
    28561 tcttggtttc atgatgacat tcgtaatgaa tgtttgtaga caactaaaga tcccaccgac
    28621 tacccagtaa agagtgacac cagctggggc actgatcgac atgaagacga tcatagcggg
    28681 tgatacgatc aacatcgtac gcatcgtttt cttttgttct tcagggatac cgatagttga
    28741 gatatagcct tgaagtaaat aagcaatacc ggcaatcgct actaaaacta agctagggtt
    28801 acctaggtcg atcccgtaaa aggttgattc acggatgcct tctgtatagc gagcagtgaa
    28861 atatagcgca gaaaagatcg gcatttggat cagaagcggt aaacaaccga taccaccagt
    28921 catacttaag ccattttcac gataaacttg ttgcatttca gcttgtgcag ccatttgttc
    28981 ttctcgagtt gtcgcagctt tcaatttttg ttgcgtagct tcaacttgtg gtttcaatgc
    29041 ttgcatcttt tcagattgga tcattgtttt tttcgattga ctaataccta atggcaaaat
    29101 gatgatacga acgatgactg tcatcaagat aacagcccag ccgtaggacc aattaaagtt
    29161 ttccactaaa tatgtaatcg cttgtcccat tgggtgaacc aatatacggt aaatgattcc
    29221 tgaagtgtca gggttaccat ttgaatccat gcggacacaa ccggacaaga aaagcatgat
    29281 cgtgacaagc ccagagccca ataaccaatt tttccattta ttcataaaat acttcctctt
    29341 ttataatttt tacacaagct taactatact aaaaaagcgc tcataattca atcaacttca
    29401 tagaaatttc atagaaataa ccgattggag gatcaaaaaa gacgcttaat gttaaaaaga
    29461 agaatccagc taaaaatact tcagtataaa gcatacttag cacctccgtt ctctaaaata
    29521 agatgagcaa cggatatttc cttgaaatca taattttagc gcaaaaaaaa gaagtggaat
    29581 gtcaattttt tccacttctt tttttttacc attgagccga tccataaaaa agctgactgc
    29641 catggtataa ggggctaact atccctgtgt cacttgttct aactctttca ttttctggac
    29701 aaccagtgct ggtgtgatcg tttctttcat gaagtggatc atttcatcag gtaaacatgc
    29761 acgttcagct acggcttgat agtcactttc tgttaaatgt aaacccatat cagctaaact
    29821 cgttggtaag ttcaagtgat gataaaacgg cagtaggtgt tcgatttctg ctaatttatt
    29881 ttcgatcatc agctggacaa ggataccgta tgcaactttg tttccatgaa gttgctgatg
    29941 gctatcaggg agtacggtca tcgcatcgtg aatggaatga gctccagctg ttcggccgta
    30001 gtcatcgcca aacccaccaa ccataccagc caataaaatg tttgtttcca cgacatcgat
    30061 aaaagcttga ttgatttttt gctggtccat cgcttccatt gcctctgcac ttttctctaa
    30121 aaggatttcg cggcatcgta acgctgcaaa ttccgctact tggacttcga taggcttgat
    30181 agttaactga gagatgattg cgtgagattc ataccatttt gctaaggtgt caccaatacc
    30241 ggcaatcatc atctcgcgag gggagtgcaa aatgatcttt ggatcgatca acactaaatc
    30301 atttgcttta gggaacatat catagcgaag catcactcct tcttcattat agaccacact
    30361 aagtggagta taagctgcgc acgtcgcagc taaggtaggt aagatgatga ttggtaagtt
    30421 tgtcttatag gcaatcaatt ttcctagatc tgctactttc ccgccgccga ctgcaataat
    30481 accattgaaa tccttctttt tgcataaaga aacaaaactg tcagcaagtg gatcggtaca
    30541 ttctccacca taatattcaa aaatttttgt tattgataaa tctggaaaaa catcttttac
    30601 tgctttccaa gatgtttttc catggagaat caaaacgttg tcaatatgcc ttttgtaaag
    30661 gtgttcaggg agtgaatccc atgcgttttc ttttatttca tactcttgag gggcaccgcg
    30721 aacaattaaa ttttttgtca tataaaaaac ttcttccatt atttttttaa attaatcttt
    30781 ttttattata atagttatgc tagtatatta gcaagttatt tatgaatatt tattctttta
    30841 tagcttttct gattacatta ttattcaaag gtggccaaat ggatgaaaaa aggaataaac
    30901 atattattag tggcgctgat gtttctactg gtttttttac cagtaaaagt acaaggtgca
    30961 gaaaaagatg aaatttacga gcggataaaa caaagtggag aattagtcgt tggcctatct
    31021 gcagactatg cgccctatga attccatgcg aatgtggatg gtaaggataa aatcgttggt
    31081 tttgatgtat ccattgccga aaagatcgca aaggatatgg gtgtgaaact aaaagtagaa
    31141 gaacttggtt ttgatgcatt attaggtgcg ttgaaaactg gcaagatcga tttagttata
    31201 tctggtatgg cggtgactga agaacgttta caagaagttg acttttctga cccatatttc
    31261 gtggtacagc aaaaagtact cgttcgtaaa gaggacaaaa ataaatttaa gacgacagcc
    31321 gacttttctg gcttagccgt tggggcgcaa aagcaaacca cacaagagga gttagtgaaa
    31381 aatgaactga ctggtgcaga gttgacctcc ctgcaaaaag ttcctgatct gatcaataat
    31441 ttgctgaatg aaaaagttga aggtgttgtg ctggaacaac cagttgcaga agcatatgcc
    31501 caacagaata agaaagtagc tttgactcca gtaaaatttg cgcaaggcga aaaaaccaca
    31561 gctattgcga tgtcaaaaaa tacaccagaa ctgaaaaatc atgtcaatgc gtcaatcaaa
    31621 acgatcaatg acgacggctt gctgaaagat tatcaaaaac aagcgaatca attgatgttc
    31681 caagatgatc agtcctttct tcaaaaatac ggtccgtatt atttgattgg aacaaaatac
    31741 actattttat tagcttttgt gggtgtattg tttggaatgg tctttggtag tttgcttgca
    31801 ctgatgaaat tgggtaagaa taaattttta aaggcattag ccattattta tatcgaatac
    31861 gtacggggaa cacctctctt agttcaaatc tttatcgttt acttcggaac aggagtactg
    31921 ggattggatc tatcgaaact ggtagcaggt tgtattgctg tctcgttgaa tagtgctgct
    31981 tatgtagccg aaattgtccg tgccgggatc aatgcggtca acaaaggaca gatggaagct
    32041 gcacgttctt taggaatgga ccagaaagaa gccatgcgtt tcatcatctt cccacaagca
    32101 atcaaaaata ttcttccagc tctaggaaat gagtttgtca cgatcatcaa agagtcctca
    32161 gttgtttcgg tcattggggt atcggaattg atctttcaaa cgggcgtggt acaaggtgct
    32221 agcttcaaac cattcctacc atatgtgttg gtctctttga tttatttcgt gttgaccttc
    32281 agtatttccc gtatattagc tttagttgaa aggagaatgg gtctgaatgc ttaatgtaaa
    32341 aaaactgtca aaaagttttg gcgagaatga agtcttaaaa tcaattgatc tttctgtgaa
    32401 agaaggagaa gtagtggtga tcattggccc ttcaggaagt gggaaaagta caattctacg
    32461 ttgtatgaat ctgctggaag aaccaacttc tggtgaaatc ttttttaagg gacaaaacat
    32521 tacagcccct gacagcgata tcaatcaaat tcgtcaaaaa atgggaatgg tatttcaaag
    32581 ttttaatttg tttccccata tgaatgtact cgaaaatttg accatcacac ctgtaaaaat
    32641 caaaaaagaa gaccctaaaa aagcaaaaga acatgctcta tcattattga accaagttgg
    32701 tctaaaggaa aaagtggaca gttatcctac cagtttatct gggggacagc aacaacgggt
    32761 agcaattgct cgagcattag caatgaatcc tgatgtgatg cttttcgatg aacctacatc
    32821 tgcacttgat cctgaaatgg tcggtgaagt gttggcagtc atgcaggatt tagctaaaaa
    32881 agggatgacg atggtcgtgg tcactcatga aatgggtttt gctaaagaag ttgccgatcg
    32941 tgtgattttt atggctgacg gcatcattca agaagaggga acaccgaaag agatttttga
    33001 aaatccgcaa aatagtcgaa cacaggattt cttgaacaaa gtgttgtaac aaagatggag
    33061 gaagtaagat ggacaaaagt aagattgcac gccggtttca gcaaccagca gaaaatctgt
    33121 tgatgaatat tgctattttg gcaaaggaaa aagaggatat tatcgattta tcgattggtg
    33181 atcctgactt gatcactgat ccgacgatta ttgaagcagc ttatacagat gtaaaaaatg
    33241 gtgctactaa ttatactgcg cctgatggca gcaaagaatt cttgtcaacg gtcgtagatt
    33301 tttatcaaaa gaaatatgga ttgagcgttg aatgcaacca agttcgagct acagtcggag
    33361 caatgcacgg gatgtatcta gttttacagg cattactaga tccagaggat gaggtcatca
    33421 tccatgaacc ttatttttca ccatatcggg aacaagtcat tttatctgga ggcaagcctg
    33481 tttttgttcc tacctatgaa gaaaagggct tccagttgga tgtggaagat ttaaaagcag
    33541 cgattacgga tcgtacaaag gtgatcgtca tcaattcgcc gaataatcct acgggagctg
    33601 ttttttctga aagcacgttg aaaaaaatcg ctgaagtcgc aatcgaaaat gaactctata
    33661 tcatttccga tgagatttat gaagactttg cctttaaaga gaagttcgta ccgatggcgc
    33721 gatttgcgcc cgataatacg atcactgtca gtggtgtatc gaaggggttt gcgatgactg
    33781 gctggcgctt agggtacatg atcgcaccag catacatcaa tcaaattgct gggttgatca
    33841 atgaaaacat cacttattcc gcaccgactg cttctcaaag aggtgcaatc tacgccttaa
    33901 agcactatga acagcttgta ccgccagttg ttgaagtatt caaagaacga ttagcttata
    33961 ttgagaaaag agtggcagaa atcccttact tatctttatc ttctgtttct ggaagcatgt
    34021 atgcttttat taatatcagt aagagtgggc tcgattcagt aacgtttgtg gaagaggttt
    34081 tagaaaaaag cggcgtactt tttgtccctg gaaaagcgtt tggggaaacg atcggggaca
    34141 actatattcg actagcagcg actcagccat tggaaatctt aggtgaagcc tttgatcgtt
    34201 tagcaaaact aaatttttag ataaaactgt atatttgaaa acagctatgg gtatccatta
    34261 gaacaaagag ggaaaatgat cctttcgaga gttggcttgt tgactgggca gcgtcaggat
    34321 ttgacaatga aaaagtggta gtggaaaatc aaagtcgatt ttccactacc ataaaatatt
    34381 atagactcca ttttattgaa ccaaaatttt tgaagtcctc ccgcttttac gtatgcctat
    34441 cactggttac taaactcgct aataagaatg tttttttgtc gatctaggat ggagagaggg
    34501 gcaaactttt tcgatctaca tacagacagt ccgatttttc tgacatttcc tattcatttc
    34561 tagaaggtca gcctattaca acaaactttt ttctgtcctc catcatcgga ttttcctgaa
    34621 ggtctacata ttggacacgt ccgctggggc ttggtgatcg tttgacttct tccacgaatt
    34681 ttttgattgc ctcatcgtcg cccgcagctt cgatcgtgac agagccgtca tcttcatttt
    34741 tcactgttcc ggtgatccct aattgatccg ctacgatttt tgtcatatag cgaaaaccaa
    34801 cgctttgtac acgtccttgc acgttcatct tgattttgtt cattttattc accgttcttt
    34861 cttttatttt taaattaatg ataccgactt ttttgctact gaacaagtgc aatgatctaa
    34921 tccatggtat aatcagttga ggtgaaaaat gtgaaagaga tccaatcagc aaaaaatcca
    34981 agaatcaaag aattaaaaaa attacataag aaaaaatatc gtgaagaaaa aggggaatat
    35041 ctgcttgaag ggtttcacct gatcgaggag gctgttgcct caaatgcgaa ggtcaacgaa
    35101 ctttttatta cgcaacgcgg tctgactgag tgggctagtt ggcttgaaga aaatcaggcg
    35161 ctttctgtta cactagtatc agaagaagtg atggcaactg tttcagatct accaactccg
    35221 caagggatca tggcagtggt ggaagctgaa aaaagagaga taccgaattt tgaaggtggt
    35281 tggttgttgc tcgacaacgt acaagatccc ggcaatgtag ggacaatgat ccgcacagcc
    35341 gatgcttttg gtctatcttg tgttgtacta ggtaagggaa cggcggatgt ctacagtacc
    35401 aaagtattaa gaagcatgca ggggagtaac tatcatttac cgatcatcca agaggatcta
    35461 acaaaactat tgcctatcct taaagagcaa ggatttataa cctatggaac agaactgaat
    35521 gaagaagcag tgtccctgaa tgatatccag acaacagaaa attatgcgat cgtgatgggg
    35581 aatgaaggtc aaggcgtacg tccagaagtg ctggcgctaa cggataaaaa tgtgtatata
    35641 ccgattcaag gaaatgcgga gtctttaaat gtaggagttg cagcgggcat catgctttat
    35701 cacttctccg tatagttaag gaactattaa gctttctgca ttttgcgaaa acctcactag
    35761 aatattacga acactagtgg tatactaagt tcaatttcaa agataaatgg aattcaaaaa
    35821 ggtggcagat cgataattat gacagaaaaa tggcgtgaag acgaagaata tatgtcttat
    35881 ataaatgatc tattagaaac tgatgcagta aagaaattag caaattacac gcaacatgtc
    35941 cactcgacac gattagagca ttcaatcagt gtgtcttact atagctacaa attagcgaaa
    36001 aaatggggcg gtgatgcaag agcgactgct cgagcaggcc ttttacatga tttattttac
    36061 tacgattggc gtacgacgaa gttcgatgaa ggtacacatg cgtatgttca cccaaggatc
    36121 gctgtgaaaa atgctgaaaa aatcacggag ctttcagatc tagagcgtga tattattttg
    36181 aaacatatgt ggggagcaac gattgcccct cctaaatata aagaaggtta tatcgttact
    36241 tttgtggata aatattgtgc agtcaaagaa gcagctcaac caatgacagc ttcaatgaaa
    36301 caaaaatggc aacgatattt cggtaaagaa caatcgattt aaggtagtta actaagtatt
    36361 tgacccgagt attttgaaaa aaatcctcgg gttttttgtt tagacattga ctaattggtc
    36421 tttttagtgt ttactttgtt gagatgaaaa agtacataac caacataaaa ataaaggggg
    36481 tgatgataag atgcgatgtg tatcttattt aaaagagttt gagggcttta tctaaaggca
    36541 ctttgataga aagatcactt ttttcttcaa agtagccgat tttgaaagaa gaaaggactc
    36601 aaagtcatgg aaaaaacaat caaaccatat caatattatt ttatcaagcg agaagtcgag
    36661 cagctgttaa gcgcctatag ttcagtgaat gatccaaaga cagtccaaac agtccaagca
    36721 ttagctgcgg aaaagatcca cgaggtactt ggtcatccta atacagctgt aaatgaattt
    36781 atggaaaagg tactagacgt cactctaaaa aaagaaaaag cagaacgact attagaggaa
    36841 ttgaaagtga tcgttcttcc attcatacaa ccgacaaaac cgcagatcga taaattattc
    36901 cgaaaagtga aaaagttaaa acaaccagat tggaatcaac tggatttaag ggaacatact
    36961 tatattggtt ggaatgatcc aggtagccaa aaaaaattta tgatctatta tgaagaagaa
    37021 aaactaaaag ggatcagtgg aacattatct cctacaatca acaaaggaat ttgttcgatt
    37081 tgtcaaagaa cttcaaatgt ttcactcttt ctttctacca caaaaactgg ttcagatgga
    37141 acatacacga aaaaaggaaa ttatatttgt catgatagtg accaatgtaa tcagcagttg
    37201 gtacaattag ctccttttta cgaattcaca gaacgagtga aacgtgagcg gtgatttcat
    37261 tgtatttttc tgaaaaatat gctatcttat ttggtgaagt gaataggtga tggtgttcgc
    37321 ctttaaacgt tgatcgtaag ataactaatg acgcctacta aagaaacggg gctacccctt
    37381 tttttagtag gcgttttttt gaggaggaaa tgagatggaa aatcgattac ctgatccatt
    37441 gaaggtgatc atctatatga tcaaatggtt attgatcaca gcaattttag gtatttttat
    37501 gggaagttta tccgcattct ttctaactag tttgactgat gtgacagcta ttcggctgga
    37561 gcatccttgg ttactttttc ttttgccgat aagtggggga ctttttgctt atctatatac
    37621 gacgtatggg ggcaattctt cccggggaaa caatttagtg atcgatcaag ggaatggagg
    37681 aaatgaaaag attccattac ggctgatccc actgacctta tttggcacca ttaccacgca
    37741 tctttttggt ggttctgttg gtcgggaggg tacagcggtc caaatgggcg gggcacttgc
    37801 tgagaatttt gctcgttggc taaaattgaa caaaatcgag cgtgagattc ttgtcatcag
    37861 tgggatcagc gcaggtttta gctcagtatt tgggacaccc ttggctggga cattgtttgg
    37921 attagaagta ttggcaatcg gaaaagtgag aaccgaagca ctgtttccaa gcttttttgc
    37981 agcacttttt gcgaactttg taacggaaag tttcaatgtc aagcataccc attatcaaat
    38041 gggggcgatt cctgcatggg acccttcact tttcttcaaa ttgttgcttg cgggcattgc
    38101 atttggtatc attggctggc tctttagccg ttcgatcgtc tggttaaaag cttggtatgc
    38161 aaagcggatc ccccatccag ttttacgcaa ctttattgga ggagcagtcg tggtactcgt
    38221 agtagtgatt ttacagacgc aaagatatct cggattaagc ttacctttat tacaagatgc
    38281 atttaatgga agtgctcatc cttatgattt tcttgggaaa cttggcttta ccgttctatc
    38341 acttggcgcg ggataccaag gaggcgaagt gacccctttg tttgagattg gggcaacttt
    38401 aggtgcgaca ttagcaccca ttcttcatgt atcagtacct tttttagcag gattagggtt
    38461 tattggggtg ttctctggag caacgaatac accgattgct tgctttgtta tggggatcga
    38521 actttttgga ggtcaagcag ccagctactt ctttatgatc tgtttgatta gctttatgtg
    38581 ttcaggaaat agtggaatct attctgcgca aaaagttgaa gtgaaaaaag gaatcttgtt
    38641 cctaccgatc cttggaagaa tctcacgaca aaaagagaaa taaaaagagg ttgggacaga
    38701 agtgtttaac tccgagaaat aagaagaaat tcacgaaaat tgctttttaa atttttgtga
    38761 atttcgctta tttccgaagg agttgcttct gctcccaccg tttattcgga gttagagtct
    38821 gtggcaaaac tgatttttag ttttgtccca cgctcttttt tattgagcaa atttgagaat
    38881 aagagcagaa aatactgcaa agccaatcat taagccacag atgataagat ggcgtttttt
    38941 gtgtaagaca aggatggcaa taaattctct taagatagca ttcggtaagt agtatagtgc
    39001 agtttttgca ccgattccgt ttgcattgag tttcgcttgt cgcgcatata gtccagctct
    39061 aaagatatgg taattatttg aagtaaaaat cgcgcgataa ggcccattca tttgctgatc
    39121 cattatttct tttgaaaaca gcatattttc tagcgtcgtt gtggaattcg tttctactaa
    39181 aatatcacgc tcgggaatcc ctttttctaa agcatattgt ttcattgcaa tggcttcagc
    39241 tactttttca tcggttcctt ttcctcctga catgagcaag actggcggat tcaaagttgc
    39301 tttactttgt gcccaataaa atgcgatcgc cttattgatc cttttagcaa gtaggggaga
    39361 tacgcgttca ccatcaatca agccagcgcc gagaacaacg atataatctt ggttatacct
    39421 tggacggttg aactgataca aaattgaaac agtcataaaa ttaaagaaaa cgatgagtag
    39481 ataagcaaaa ataaacggaa tgacagcaaa tagagcattt gcccattgtg ggagccaatg
    39541 gactaatgta tagtccaata tcaaaaagat agtcaaagcg actcctaaca agagagtcaa
    39601 aaggttggcc aaagaacggg attctttttt catgactaca acaccattcc aaaataaaaa
    39661 aatgatcaaa atgtagatac cgattgccaa aagaattaat attggtatcc cgatcaaagc
    39721 caataaccca ccgataaaga tattttgcgt ctctattagt agccacccta gataaccacc
    39781 aaagacaatc aaaaacgtat taaaaagtac gccattgatt aacctacgtt tttccttaaa
    39841 atagaaaaat gcaaaaatac tgaaaaatag caatggaatc aaaaaataaa atgtatgata
    39901 aaaaacttct aaaacgacga agtaagtgac catcatcata aaagtccacg tcccacaata
    39961 aaagaataga gcgggatcgc tttggcgctt gcgccaatag aggatgcctg acattcctaa
    40021 aatatagata actaaaaata tcaggtctct agtaagattc attgaatgaa ctcctttcga
    40081 tttgtaaatt ttttcttaaa aaagatagca tcatttagta gtaaatatat cgaatataga
    40141 aaagttagtt actaaatagt caatcagttt tttcacgacg tttctattat aacgaattat
    40201 gttgaaaaaa gcttttatcg aaaacataaa tcttcaaatt aacagatttt ttagacctcg
    40261 atcatgatga agacttttgt agatacaaat aactgtaaca ttgaaaatga atgcgaaaac
    40321 gatatttttt tttattggaa aatatcggtc aagcaataaa ccagttgcat aataaaatgt
    40381 taacggcaac aaaatctgtg gaaggaatga accggatgat ttgatggaaa aaacaagata
    40441 gacaagaagt atacatagta aaacgtggaa caagtaggcc agaatgaaaa atcagttttt
    40501 atccactcgc ttttctatac attcactgtc acaaatataa tcgaaagtaa ttctattttg
    40561 agataaagta ttttatagga gatagtatat gaacaaaaaa agatttaaac gtattgaaag
    40621 aaaatttgca ttaacttatg accaagctaa gtatttgaaa acactatttc caaaacaggt
    40681 agttcgggat aaatatagtg atcattttat ttcttccttg tattttgata caaacgatca
    40741 tcacttttta aaagaaatca ctacagaaaa gtcctatcga gaaaaattta gaatcagaaa
    40801 atatggagat cctcaggaca tatcgcctat ctacttagag ataaaaagaa agtttcaagg
    40861 aatgacagat aaacgtagaa taaaattagc gcatcatgag ctcgatgata tgttggaaaa
    40921 tatcgaattg gcagaaacca cattcagtaa tttagaagaa gagtatattt ttaatgaaat
    40981 acagtggttg ttcagaaggt tggcattgga gcccaaagta tatatctatt atttaagaga
    41041 agcatttgta aatagagatt cagaagattt gcgaatcact tttgatcaaa atatccatta
    41101 ccgtctaatg aacttaacga cctgggaaca ctgtacagaa gacaatctgg tagcgccaga
    41161 aatcgatgtg ctgatggaag taaaaacgac aggagaaata cctgaatggt tacaggtagg
    41221 attggatagg atgggtaaaa aagagataaa attttctaaa tataaacaag tctataaaag
    41281 atatatccaa gaggacccgc tctatgttca atagtgtctt aaaaaatgag ccttctcaac
    41341 tcacactaga tgatttcttt ttatgtatgg tggtgtcgat tttgctaggc attctgattg
    41401 cttacaccca tatgtatcaa aacaactgta caaaaaactt catgtttgcc ttgattgggt
    41461 tacctacttt agtacatacc gtcattttgt tgatcaacgg taacttaggc acggggatcg
    41521 ctgttctagg cgcatttaac ttagtgcgat ttcggtctgc tcaagggaat tcaagagata
    41581 ttttcagtat tttttgggca acgtcgattg gtttagccac agggacggga tatattgtct
    41641 atgcactatt cgtgtctagt atagttgctg ctatccaagt catcatcttc acgcttccaa
    41701 tcaaaaataa acatacagtt tttaatactg aaaaaacgat caagctaaaa gtttctaaat
    41761 ccttgtttct ttcgagtatt attgaagaaa aaattgcaga atatacagaa aactgcaatt
    41821 taaaaaaagt aaaaatcaat aaagaaactt gttccttaca ctatatagta aaagtaaaaa
    41881 cgagtatgct tgaaaaagaa ttggttcgaa cgatcatgga tcatccagaa gtcatttcga
    41941 tcactgtagg tatggataaa tcgaataaat tattgtgata accaagaaag tacttaacaa
    42001 tgaagtaaga ccccagttcc aaagtttagc ggggacactt caataaagta ctttcttttt
    42061 tgtcttcagc aactactggt aaaactgaga gcaagctttc gtgagagtac agcgataaag
    42121 atcaacctcc ctttttatct cgagcaattt ccttgcaagg ctgtttcgtt gatcttaaac
    42181 gagttagcaa cagtcaaaga tgcgatggcc ataaaaaaat tgcattaagt tggctggtat
    42241 ctgatagctg ggaatctaag gaagactatc cattctgttt cttaaattcc gctagttgat
    42301 agatatcagc caattgttcc agatgatcaa catcaaagat aaccgaaaga tgtttccttt
    42361 tattcccatt tgcttgaaga gtttttatct tctctttggt tccttcactc aatgagagtg
    42421 tgactaattt ctccctcgtt ttttctggga aatgaaagga tactttgaaa aatccgtccc
    42481 aaatagaaag ccagaagatc ggttttactt tctgagagtg ggacaaaagt aagaaacact
    42541 tttgtctcat tctttaaaaa cgaataaacg gtgggagcag aagcaactcc ttcggaaata
    42601 agccaaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttctcggag
    42661 ttaaacactt ctgtcccaac ctcttgtatt caccttttga tagccaagct tttccatcat
    42721 tataaaaccg caaatttcaa aaaaaaacga acaatacaaa aagcgtattg ctcgtttttc
    42781 tcttcagttt ctcacaccta aacgactttt gtctcgacct ctttttggtg actattcatg
    42841 atgttaaaat aaattactga aaaaatctgt gacatggcgc caaccaatga caaaaatatt
    42901 agctttttca acagtagaag tggtaatgat gtctgtcgaa ggctcttggt ttgcttccaa
    42961 atagcctaat ttgtcatcag ctaattggat cgttgctgag ccaactgtag tgcctttgtt
    43021 gataggagcg gtcacttggt catccgttac tttcttttta tcaattgttg gagtaatcgt
    43081 taattttcca gtatccattc cattttcgac ccagactttt acagggcttt ttagcgcaac
    43141 attgacggag gtttcttttc catctttcac tttgatgtct tttaggcttg ggatgcttgc
    43201 atttgctttt ccaagttctt tttctgacca gttgtcataa caataatcca tcaatcgtga
    43261 tgtttcattg aatcgggcac ttggattttc ttcatggtta tctgcattta aaacgactgt
    43321 gatgattcgt cggccatctt tgttgatcgt tccgacaaag catgcacccg caaaatcagt
    43381 tgtgccagtt tttaatccat cgacaccttc gtgatagttt acaaaaccag gtagcatcca
    43441 gttccaattg accatttcaa ccggtgactg tgtgttttcg ccgaatgttt gatttgtggt
    43501 actcgtgatt tttaagactt caggataatc taagatgagg tgacgagcaa caatagcgac
    43561 atctttcgca gacagttgat tttcatctgt ttcaccagta ttctcaggtc gattctcacc
    43621 taggtaagaa ttgtttaaac ctgatgcatt gacgatcgtt gcatctttga taccccaatc
    43681 ttttagttgt ttcttcatta gctcaagaaa tttactttca ctgcctgcaa ctttttcagc
    43741 taaagcaacc atggaagcat tggctgattg gatgaaagct gagtcaacaa gttctttcac
    43801 tgtataatca ttttctttat gcaaaggaac attcgataga tccggcacga cacttaattt
    43861 ttctgcataa tccgaaatgg ctaccttatc ttcccaagat aatttaccct ctttgatttg
    43921 ttcaagtacg atatatagac caatgatctt tgtgatggaa gcaattccca tcgttttttc
    43981 gccatcctga tcataaagga tttttccact ttctgcatct acggcaaagg cagctttggc
    44041 atttatttta aatgcgtcgt ctgcttgaac agttgcgcta ggcaagaaag aagtgataca
    44101 taagataaga cttgctaata gtacaataaa acgggtattt tttttgaaca tctttgttct
    44161 cctcattttt aaattgattc ttacaactta gaatattatc agagagaaca aaaggtttca
    44221 atggttctga aaaaaacctt ataaagaaca agtcttatta atgaagaatc atagaaagtt
    44281 atcttttggg ttcattgaca cgaactttga ctggctgaac ttcgggttcg gttaggcgtg
    44341 atgctgcaaa aagggcgcct gctgagagtg cgaccaaact aaaaatcatg aataattgca
    44401 taagatcacc tcgttctttt ttgttttttt atagtatagc aagagagttt gaagctactg
    44461 ttaaggaaat gagcatataa ttgatgaaag tatgaaaaaa atatgaagta ggacaacaca
    44521 actaaaaatc gatcaggtat ttctctggta gggattcgtt ttattgtatt gataaaatgg
    44581 gatgtggtag actttattta cagatacaaa gaaatgattt ttttaggagg ggtacgttta
    44641 tggcaaaaaa agaaaaagaa ttattatcac aattgacttc agaaggtacc aaaggaccgt
    44701 ttgtcacgat tatgttaaac acccatgttg cgcatcaaga cgtagaaaaa gatcaaatca
    44761 aattcaagaa ttttgcaaag gaagcgaaaa agcgctttga aaagaaatac tctgagcatg
    44821 attggtctcc attccaagaa aaaattacta gtttgttgga cgatccgtct ttctggcgta
    44881 gtgccacaac gagtgtagct gtcatagtat caccagaaca agtgtttatc caccgtttgg
    44941 gtattcctgt agatgaccag tattatgtga gtgatctacc ttatttatta gcgatcatca
    45001 aaaatggaca tttcaactat tcatactatt tattaggatt gaatcgtgat tcgatgaaat
    45061 tgtacgaagt aacgaataaa caagtcaaag aagtggcttt gcctgaagat gcaccaagag
    45121 atgtcgtcac tgctttagga tctgaactaa caggaggcgg gttgaactac gcatcaaatg
    45181 gtagtgaagg tacgacgttc catggagtca atcctaaaga tgaagaagtc gaaattgatt
    45241 gggtcaatta ttatcaagca gtcgatgact atttgaaaga tacatttgaa aatccagaga
    45301 aatggcctat cttcttattt gcattaccag aaaatcagac gatgttcaaa aaagtggcga
    45361 agaaccctta ttatcgttcc gatgttgcga tctcagcttc acctgcacaa ctttcattaa
    45421 aagaaattga agcaggcgca aaaaaactat cgcaagaatt agctgtcaaa gaagcaagtg
    45481 actacaataa gttgcttgat aaaaagttca ttgatcaact cgttgatatt ataccggcag
    45541 caaaagaagg gaaaatatcc catttattca ttgctacatc taatttagca gatggttttg
    45601 gcgaagatcc agaaacagaa tatgaccgcc gtcaggtgct gaatgaacta gcagataatg
    45661 tgatcacgaa cggtggtcag gtctttatcc ttgaacaaaa agatgcacca gacgaaaaga
    45721 gtttagttgc gatcttacgt tattgagtga aaactaccaa agtagatgaa tggtcaggcg
    45781 ttgatagaag tgtatagctc cgagagaagg agtttcctct gtttcagccg ttggattcat
    45841 cttgagcgag agacaaaagc tagcttttgt ctctcgctca ttatttttgt gctttagtcc
    45901 cattggcttt gcgaggggaa gatacttttc tcaaaaaaac gaaccttgca gttgagaggg
    45961 ggatagatgc gcatactaat ttttatcagt taaaaaatga tgtggatcat tagagaattc
    46021 caaagttcgt tcatcctttt tcagataata aattgaaatt ttttgcaaaa gtgaaaatag
    46081 caaatagaaa ttttctgaat cttatagggg tgatttcgag aaaacgttcg tttatttgca
    46141 aatatatctt ctaaataaga gaataaatac aaaaaaataa ggaaaaactc tggcaatttt
    46201 gtaaacggta tgatatagta agaatgtagt ttttgttcgg taatctgtgg tagcttgttt
    46261 caaataaaca tcttccagat atacaccatt caagtaattg caaaatatgt gttgatacac
    46321 aaggaggatt tttattatgg aaacaggtac agtaaaatgg tttaactcag aaaaaggatt
    46381 tggattcatc actgcagaaa acggaaacga tgtattcgtt catttctcag ctatccaagg
    46441 cgacggattc aaaactttag aagaaggcca agcagtaact ttcgacgttg aagaaggtca
    46501 acgcggacct caagcaacaa acgttactaa agcttaattt acctatatta aaaataaaag
    46561 tccagacatt tatgtgtctg ggcttttttg tctttttttc cttaaaaagc gatgagagta
    46621 tttttctgca ctttgtagta taatttcaaa agaacattag aggcaggaga agaggaagat
    46681 attttgaaac caagaactta tgcagtcgtt gatatcgaga cgactgggac gaatccaaaa
    46741 gaagatcgga tcatccaatt tggctgcgtc atgattgaag ctggtcggat cacttcacgt
    46801 ttttcgatcg atattcatcc agggcggaag atttcaaaac aaatccagca tttaacaggg
    46861 atcacgaatc aacgcgtcca aaaagcgcct tattttgaag atgtcgccca aacgatttac
    46921 aatcttttgg cagacaccat atttgtcgcg cataatattt attttgatta taattttttg
    46981 aaccatgaac tcatgcgatg cggacttcct agtttgaaga taccgggcat cgataccgta
    47041 gaacttgcac aaattttctt gccgacggaa ccaagttttc gcttagctga tttgtcagaa
    47101 agccttggct ttattcatga gaatcctcat caggcggata gtgatgccga agtgacaggt
    47161 cagcttcttt tactgatcga agaacgaatg aggaaattgc cgatcatcac attagagcag
    47221 attgcacgat taagtagcca tacaggaatg gatacgagtc gtttcattta ccacgtgctt
    47281 gaagaaatga aagaaaaccc ggagcccctt gatctctcat tagaaatcat cgatgggcta
    47341 gctttgcaga aaaaagaagt ggagctattc acatccgtcc attatggcga gcggacatat
    47401 ccaagaaaaa aacaagcaaa agaaaaaatg tttggtaaaa cactgatgta tcgaaaagaa
    47461 cagaacaggc tgatgaatgc tgtctacgac catttcacaa aagatgaatc aaaagatttg
    47521 atgatcgaag cagctacagg aatgggcaaa acgattggtt atctgttgcc tttgagttat
    47581 ttggctacac ctgaaaaacc agctgtcatc agcaccgttt cgcttgtttt gcagcagcag
    47641 atcatggaaa aagacattcc attactgaat caattgctag atcagccgat acaacctgtg
    47701 attatcaaaa gctatcgcca ctatattgat ttacagcgtt ttaaaggtac acttgtggaa
    47761 ccgccgggac aaaaacaata tgctttgtac caaatggctg tcttagtttg gctaacacaa
    47821 acgaagaccg gtgatttgga tgaattacat ttaaccaatc tgaaccatgc attctttgca
    47881 gatgtcgcgc acagaggcac tgggttttta gcaaggaatc agtcatttta caaacaagac
    47941 tttgtgcgtt tcttgcaaaa gaaaatcaaa caaagcaatt ttttgatagt caaccatgca
    48001 tttttagtac aagaaacaca gcgaaaagaa ccattgcttc cagctagtcc ttatttgttg
    48061 attgatgaag tgcatcattt gccggaaatt gctgaaaaag taaatgacaa acgactaagt
    48121 attaactact tccataaaca aattcagcaa ctggaagagc cgaatcagtt atttgaccgc
    48181 atacaaagtt atttgccgaa ggaacatgaa gccttgcgct ggttgtcgat ttatcgagaa
    48241 gaactctctg cactatgcga agcgcagatt tgcgtggcag agggattaat gaacttagta
    48301 agcagcaagc aacctaacgg acaatatcca gaagaagtca tgatgacaaa agaaatgttg
    48361 gatcagctgc cactggaatc tggaacagca atggatcatc tgcaactttt ttatcaagag
    48421 atccaacaac tacaatcaca gatgcgcttg acttttgaga ttatccaaga aagttggacc
    48481 aaccagatgc ggatcgattt agcaagttta tatagcttat ttgatcaatt agacaagcaa
    48541 tacttgctga tggaccaatg gctagaagac tggcgagatc atttgatcca ttggttcgtt
    48601 cccgggaata cagcacagca gatgattttt catctccttg attttcaagc agctatgttg
    48661 ccgtcaactg tgtggtatcc tcgatatgac catattcttt atattggagg aacactgaag
    48721 atcggaccta atcgacagta tctctctaaa cgacttggca ttgaagcagc acctcttaaa
    48781 gtgattgctt ctccttatga ttatgagaaa caggcaagaa tctatgtgcc gaaagaagca
    48841 cctgcagtcc atcaagtcag caatcaagca tatatcgatt atttagaagc agcactcaca
    48901 ggattgattg aacaagaaga tcggccgatc cttgttttat tcacttctca tgatgttttg
    48961 caaaaggtat atcagaaaat gcatttgtct atgctagaaa aaggacgtga aattcttgct
    49021 caaggatttg gcgggagtcg ggaaaaactt ttgaaacgct tcattttgtc agaaaaaagc
    49081 gtcttatttg gtgctgacag cttttgggaa ggcgtagatt tacctggaga tgctttgcag
    49141 attgttgtag tcacacgttt acccttcgac aacccgttac gtccgatggt caaagctagg
    49201 aatgcttatc tggaatcaga ggggatcaat cctttttatc aggaatccgt gcctaaagca
    49261 gctttgaagt tacgccaagc attaggtcgt ttgataagga cggagacaga tcgtggcgta
    49321 ttacttattt tggatcggag attagtcact gcaaaatatg gtgcacgaat catccatgca
    49381 ttaccgaaaa aagtaccgat aaaagagcta gctatagatg aaatctatca ggatatccat
    49441 gaattcttaa agaaaaatga agaaggaagt taaaggtgcc gtcttttttc atcattaaaa
    49501 tctgctataa tgaaaaggaa tgaagataaa ggagcgacga cccttgaaga aaaaacagac
    49561 aaaggaaaaa acgattaatc gcgtgttgct tggcttgatc gtatttttat tagcggtcat
    49621 cgtgatcagc tcgatttttt acttgcgttc cagtcgtccg gcggtccagg cgaaaaaaga
    49681 agcagtagaa atcgcaaaga aatatgcaaa tctcgaggaa gccgatcatt tttactggtt
    49741 tactcgaaag aaaacgtact tttcattgac gggtacgaat gataaaggaa aagaaatcgc
    49801 cgttatcatt cctaaatctg gtgaaaaagt aaaagtattg gatcaaacaa agggactgac
    49861 tgaaaacgaa gcaaaacaga caattgctgc ggcccatcct gaaatccaaa tcgaaaaagc
    49921 tgctttaggg atgtatgatg atcagcctgt gtgggaagta gtaggaaaag aaacaaatgg
    49981 gaaactgaat tattacctga ttggctttga atctggagat gaagtcaaga caatcaaggg
    50041 aatttaaaag gtaaaggaat gaatgggatg gagttatcaa aaagaacaaa acgattagaa
    50101 ccgtctgtca ctttggcagc tgcggcaaag gccaaggcat taaaagcaaa aggcagagat
    50161 gtgctgagtt tgactgtggg agaaccggat tttgcgacac cagaaaacat ccaagaagca
    50221 gcaatcgaag caatccgcaa tgggaaagct agctattata caccaacagc aggaatccca
    50281 gagcttcgac aggcaatcgt tgattatctg aagaaaaact atggacttgt ctatgaacct
    50341 tcacaaacag tcgtaacaga cggagcgaaa tttgctttgt ataccctttt ccaaacgatc
    50401 cttgacccgc aagatgaagt catcattcct gtgccttact gggtcagtta cggtgagcaa
    50461 gtaaaactgg cagaaggact tcctgtcttt gtgaaagggg aagaatccaa tagctggaaa
    50521 gttactgttg agcagctaga acaagcacgt tcagaaaaaa caaaagccgt aatcatcaat
    50581 tctccatcga atccgactgg aatgatttat agcaagaatg aactgcaggc aatcggtgaa
    50641 tgggcagtaa aaaatgatat tttgatagtt gctgatgata tttatggccg tttagtttat
    50701 aatggcaatg agtttactcc aattgcaacg atttcagaag caatcaaaaa tcagaccatc
    50761 atcatcaatg gcgtgtctaa aacatatgcg atgacaggct ggcgcattgg ttatgcggtg
    50821 gggaattctg agatcatcaa tggaatgatt gctgttgctt cccagtcaac cagtaatccg
    50881 acagcagtca gtcaatatgc agctgttgaa gcattgaaag gcgagcaaga tacagtggaa
    50941 gaaatgcgga acgcctttga agaacgcctg aatacgttat atccgcttgt ggcagaactg
    51001 ccgggtgttt ctcttgaaaa gccacaagga gctttttatc tttttcctaa tgtaaaagaa
    51061 acgctacgca tgtgtaaata tgaaaatgtc acacaatggg tagaagatct tttagaagaa
    51121 acaggggtag cgcttgtgac tggcgaaggc tttggggcac cagaaaatgt ccgcatgagt
    51181 tatgccactg atcgcgctac gttagaagaa gcggtacgca gaatcaagca atttattgag
    51241 agtaaaagtc aaaattaatc ctgaagaaat tcaaggaata taaaaggaga gtcatacgtg
    51301 gaacaaattc aaatcatcga ttcgaaaaac catgtgggag aaacagtaac aatcggcgct
    51361 tgggtcgcaa ataagcgatc aagcggaaaa atcgcatttt tgcaattaag agacggaaca
    51421 gcctttttcc aaggtgtagt tgtaaaaagc gaagtgccgg aagaagtttt ccaaacagct
    51481 aaaaatctac accaagaaac atccgtgtta gtaactggag aaatccgtga agacagtcgt
    51541 tcaaaatttg gttatgaaat cggaattaca aatgtggaag tggtaagcga gagccacgat
    51601 tatccgatca caccaaaaga acacggaaca gacttcttga tggatcatcg tcatttatgg
    51661 ctgcgttctt caagacaaca tgcgattatg cagatcagaa atgaaatcat tcgtgcaacg
    51721 tatgaattct ttaatcaaca tcattttgtc aaaatcgatc cgccgatctt gactggttct
    51781 gcaccagaag gcactacgga tctattcgaa acaaattact ttgatcaaaa agcttattta
    51841 tctcaaagtg gccaattata catggaagct gctgctatgg ctttcggaaa agtcttttca
    51901 ttcggtccga ctttccgtgc tgaaaaatca aaaacacgac gtcatttgat cgaattctgg
    51961 atgatcgaac cagaaatggc tttcactcat caagaagaaa gtttagaagt acaggaacaa
    52021 tatgtagcat tcttagtaca aaatgtttta gatcattgcg aatacgcatt agatgtacta
    52081 ggacgagaca aagaagtctt aaaaacttat acaaaattgc cattccctcg tattacttat
    52141 gatgatgcga tcgaattact gaagaaaaat ggatttgatg acatcgaatg gggcgaagac
    52201 tttggttcac ctcatgaaac gttcattgcg aattcattcg aacagcctgt ctttatccta
    52261 aactatccaa aagccatcaa gccattctac atgaaaccac atccaacaag agaagacgtg
    52321 gtgatctgtg cagatatgat cgcaccagaa ggttacggtg aaattattgg tggaagcgaa
    52381 cgggcaacag actatgattt cttgctagaa caaatccgta atcacggttt ggatgaaaaa
    52441 gaatattcat ggtatcttga tctacgtaaa tacggttctg ttccgcactc tggttttggt
    52501 ctaggccttg aacgaacagt cacttggatt gctggtatcg accatgtgcg tgaagctatt
    52561 ccattcccac gtttgttgaa ccgaatttat ccataagagg ttgtgaaatc agtgccttga
    52621 cctgagtatt agagaaacaa aacggaaaaa tagcttctca tattttactg ttttttgagc
    52681 taatatcgag ggtcagctga ttgaacaccg tttatatcaa gaggttgtgc gtctgccgtt
    52741 cagctccgag tcacaaggag taatttcaaa aaaacatttt ttcatgtttg atgactattg
    52801 tgacttgtga ccaaggagtt ggccggagag caacgtttat ttaaaaaaaa gaactgcgaa
    52861 ctctcgcagt tctttttttt taaatacggg ataaaggaag tttccagcag ataagagaag
    52921 aaagcaaaag aaattgctat ttgattcaca aggttctata acgaaatcgc aatcaatcaa
    52981 acatatacaa agaaggttat tcaaatggaa atctgatttg atgatgcttc tgctgttgaa
    53041 ataaagcagc atctagcacc tgaaaacaaa ctaatagtaa cattcgaaga tggagtaggt
    53101 ccgtattttc agcatgcaat gatccatatg taagttcaat tcacccttga aatcatcgga
    53161 ccagataaag ttatggccaa tctatcccaa gcaatctaga agagatatta gtaaaggaac
    53221 atttactgga agtcttagat gagcctataa atatatgttc tgatggcgtc atcaagtggt
    53281 ctgattgacg gaagcttagg tgtgatcgat ctctctggac caaacaatga aggaatcaag
    53341 gggaatccag cacgttaaga aaaaaatagc ataaaaaact agacacttga tgagcgttca
    53401 ttcaattgtt tgaagggact aggctggtgt cattttttat acatatattt tctctttcgt
    53461 cttgataggg cttatgggat aatataagga agaaaatcat cgttttattc aaaaaggagt
    53521 ttttgacaaa tgaaaaaacg tatattattt atcggtgaca gcattacaga cgcaggaaga
    53581 gaccgagcag aagcaactga tctaggtaaa ggttatccac ttttgattgc cggaaaacta
    53641 atctcccaat atccaacgat ggaatttgaa tgcatgaacc gtgggatagg aggagacaaa
    53701 attgcagatc tgcaagtacg ctggcgagaa gattgtttag ctttacaacc tgactatgta
    53761 tcgattttga tcggaatcaa tgatacctgg cataatacta gcgatcaggc tatattcgga
    53821 actgagaaat ctgctcagca atttgaatcc gtttatcgaa aattactaaa tgaacttcaa
    53881 aaagcgggaa tctgcaacat catcttgatg gagccgtttg tacttcctat aaccgacgac
    53941 cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa ttgttcgtaa acttgctaag
    54001 gaatatggcg cattgctggt tcctttagat ggcatattca atgctttagg gattgcttat
    54061 ggtttttcga aatatacaag agaagatggg gtccacccaa cgattggcgg acatgagatc
    54121 attgcacaaa gctggataga tgcaattgcc gcagtcgatt tttttagtgg agataaaaat
    54181 gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc agtaaaagaa gatctacctc
    54241 aaatcaatca gctgattact gatcatccag aatcattcgt aaaagcaaaa acggcagctg
    54301 acctaaatga taagaataaa ctattgctcg ttgctgtaac aaaagaaaaa cggattatcg
    54361 gttttgcgca agcggacgtg caacagtcag aagaacgggt gggtcagcga ccatcaggaa
    54421 aactaatcca acacacatta gtaacaaaag aaaatacgga agaaatccaa aaaaaatttg
    54481 cagaatattt gatgctggaa gctaaaaaaa gaagaataaa atcaattttt gcccagtttt
    54541 aatcaaaaac agtaatagaa ggagaaatac aatgagttgt ttaggaaatg tcatctggtt
    54601 tatattcggc ggactaatcg gtggtcttag ctggttagca gcggggatac tttggtgtat
    54661 cacgatcata ggtatcccaa tcggtatgca atgttttaag atcgccttcc tttcttttgc
    54721 gccttttggc aaagaagtaa tttatcacca gtctggcatg tcattgatcg ttaatatcat
    54781 ctggctgatc gtttcaggcc taccaattgc tctcgcacat gtcgtcagcg gcatccttct
    54841 atgtatcacg atcataggga taccttttgc caagcaatct ttcaaacttg ctcgattggc
    54901 gttgatgcca ttcggtgcag aaattgtata aaagagaata ataaaggtta gataaagcat
    54961 agagtcaggt gggatacatg tatttcacct gactctatgc gttttcctgt ataagttctt
    55021 ccatgtgact tttacgtctt ttatttatat ggggttactg tttatggaaa gttgaaataa
    55081 ttttcattaa agaactcttt ctatattttt gaaagcgtag tggagcttcg tatatctggg
    55141 aaggacattt tgcagggatt gaaaaaagat attgtcctat cgaaaaatta atgaatttag
    55201 acaaattgcc agcaacaggc ttcaaactta gttgctatcc tgtcaagatc aagaaggcaa
    55261 gtgcgggttg gataagagca gtggcaatga tcgaagagaa aaagaaagaa taagccaaaa
    55321 catctgtatg gaagaaattt tccgatgcag atgtttttca ttttttcttt cggatacctt
    55381 gacatatgaa taaccattca tttataatta gttatgaaga gatattcata tgttgaggcg
    55441 aggaggtcat taaaatgaaa agttacagat tagaaggttt agattgtgcg aactgtgcaa
    55501 tgaaaatcga aaaaggagtt cagaaaatca atggagtcaa ggaagcaacg gtaaacttca
    55561 cttctggaaa actaacgatt gacgcggagg aagatcattt ggctacgatt gaacaagaaa
    55621 caaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt aacagaaata gataaggaaa
    55681 aagtatctga acatggaaat gaaaaagaga gaaatacgct gttccggatt ttattttccc
    55741 ttgcaggtat cgctttattg ctattatttg attttaatga actaattcgc ttgatagggt
    55801 atttattgat ttatctgctg ataggatatg atgtagtgaa gaaggcagtg atgaatatcg
    55861 tcaaaggaaa aatctttgat gagaattttc ttatgtctgt tgctacgatt ggcgctatgt
    55921 tgatcggtga atatccagaa gccgtagcag tgatgctatt ttatcaaatc ggcgagtatt
    55981 tccaaggatt ggcagtcagt cattcaagaa aatcgattcg cgaattgatg gcgatccgtc
    56041 cagaaacagc tcacgtgcag acggcggaag gattgatgac cgtgaaccct gaagacgtgc
    56101 tgatcggtca gtttgtactt gtgaagcctg gtgaacgtgt accgttagat ggaacgatca
    56161 tcgaaggaga atcattagtc gatacgtcag cacttacagg agaatcagtc cctaaatctg
    56221 tatatgttgg tgaaacagta ttaagcggat ttatcaataa aaataaacca tttttagttc
    56281 aagtagaaaa atcttatgaa aattctacta tcagcaaatt attagaatta gtagaaaatg
    56341 ccagcagtaa aaaagcacca gctgaaaatt tcattactaa attcgcgcgc tattacacac
    56401 cagtcgttgt aggattggcc gttttattag ctgttttacc tccacttgtt gtttctggag
    56461 cggcgttttc tgaatggatc tatcgagcat tgacattttt agtgatctca tgtccatgtg
    56521 cattagtgat ttccgtgcca cttagtttct ttggcggtat cggaggagca agtaaaatcg
    56581 gtgtattagt aaaaggttca aattacttgg agttgcttgc gcaaaccgag acagtggtat
    56641 tcgataaaac cggcacatta acgaaaggcg acttttcgat ccagacgatc gatacaaaaa
    56701 tcgatccaaa gatattcatg caatatattg cgagtgcaga acagttttct acacatccga
    56761 ttgctcaatc tgttttagaa ggctacgcag gtccgctttt accaacagct aatatccagg
    56821 aatttgcagg tgaagggatc ttagctgaag ttgatggcaa acaagtatta gtaggtaatc
    56881 acaaattgat ggaaagattt gaaatctctt ttccttcaag tcaagaaatc ggtacattgc
    56941 tttatctagc aatcgatcaa agttacagtg gttatcttgt tattgcagat accttgaaag
    57001 aagatgccgt agacgcattg gttcaattaa aacaggctgg tgtgaaaaac acagtcatgt
    57061 tgacgggcga ctcgaaaaaa attgctgatc atatcgggaa gcaagtaggc gtggacaaga
    57121 tatatagcga attgcttcca gaagataaag tgcagcggtt agaggaaatc ttacaaagta
    57181 acaataaaaa gaccgcattt gtcggagatg gaatcaacga tgcaccggtg ctagcaagag
    57241 cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg
    57301 tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa
    57361 cattgaaaat cgtcaaacaa aacattgtct ttgctattgg gataaaaatt ttggtacttt
    57421 ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt atttgcagat gttggggtga
    57481 cagtattagc tgttttgaat gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa
    57541 tgaagaggac gaatgaacgt cctctttttt ttatatcctt ttcttaatga aaaatgtaaa
    57601 gaaaatagat aggattataa aataactaat gtttttaatt ttgcagaaaa ttcaaaatag
    57661 cttgaaattc aataaggtca atgaaataaa agaaaataac ttattttttt gatggaaaaa
    57721 atatgaaaaa acgatgtgta ttaaataaaa taatacacac ataaaataac actgtcttgt
    57781 aagtttgtta aaaattgaat atgattacat ctggataaca aatttttatc ataaaggaga
    57841 gaacgaatga acaacaaaca attacgctgg tttactgttg cgttaattgc atttaacatg
    57901 gtttggggca tggggaatgt agttaataac tatgctcagc aaggagtatc agttgtcacc
    57961 tcttggctat tgattttggc catttatttt attccatacg cattgatcgt tggacaatta
    58021 ggatcagcct tcaaggatag taaaggaggc gtcagttcat gggtcgaaaa tacgacatct
    58081 aataaacgac tggcatacta tgccgcttgg acttactggg tcgtccatat tccttatttg
    58141 gcacaaaagc ctcaagcaat tctgattgct ggtggttggg cagtccaagg aaatggaaat
    58201 atcgtaaata cgatgtcagt acaaatggta gcagttattt cacttattat atttcttgcc
    58261 tttttatatt tatcaacaaa aggactttct actttgaaag tgattggcgg actggccgga
    58321 acagcgatgt tcgtcatgtc gatcttattt attcttttag ctgtaacggc accgttcatc
    58381 aatccaacga tggaatttgc cacaccacat atgaatcaag tgaaaacata tattccaaca
    58441 tttgatttca gttatttcac aaccgtttcg atgctagtat ttgcagttgg aggggcggaa
    58501 aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa aggaatttcc taaagggatg
    58561 atcttcttag cagcaatggt agggatttgt gctgtattag gctcagtcgc aatgggtatg
    58621 ctgtttgcta gcgataatat ccctaaagac ttgatggcaa atggggcata cagtgctttt
    58681 caaattttag gtaattacta tggtatcggt aactcattga tgatcattta cgcattgaca
    58741 aacatgattg gacaaatctc cgcattagcc ttttcaattg atgcaccact aaaaatcttg
    58801 ttagcagatg cggatccaga atttgtacca aactggctac gtaaacgtaa tcgtaaaggt
    58861 acattggtga atggttatct tctgacaggt atactagtag gaatcattat tttgttgccg
    58921 atctttggta tcaaagatat gaatgaatta gtaaaatggt tgacgaactt gaactcagtt
    58981 gtcatgccaa tgcgttacct atgggtgttc ttggcatata tgatgttgaa taaagcttat
    59041 aagaaattca attctgaata taaatttgtt aaaaatccaa aaatcggttt tatctttggt
    59101 gcttggtgct ttgccttcac tgcgtttgca tgtatcctag gaatggtgcc aaaagtagat
    59161 tatactgctg atcctaaagc atggtggttc caattattca cgaatatcat cacaccaatt
    59221 gtcttgatct tattaggtat gatcttgcca gcaattgctc gtcgagacaa aaacaaagaa
    59281 attactgaac cagaaacagt ggttcagcct taaaatagaa atacttgtta caagaggttg
    59341 tgacagaagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc
    59401 aagtaataag aacagccaaa caaaaatagc tcctcatatt ttgccgtttt ttgagctaat
    59461 gctgaaggtc agctgattga acaccattta ttcgaaacaa gggactttga cacgactttt
    59521 gtcacagtcc cttgtttttt tgcggcgtta taagtggtat gatcgaacca aattccaaag
    59581 agaagtgagg gagaagaatg aagatcatcg tgattggaca aagtgggtcg ggaaaatcgt
    59641 ccctcgcaag gaagatcaaa gaaatcaccg gctttccgca ttcaggcaaa aaagtggttt
    59701 ttgcaagaac agcaatcgtt tatagtatct aatccgtcat ggatcgttga aggaaattat
    59761 acttctacgt tagaggaaag gattaaagaa gcagataaga tcatttggct taaagtgccg
    59821 cgttatctcg ccatctaccg agtagtttgg cgttctctaa aaagaaagat aaacaaaaaa
    59881 tctcggccag atatgcctga atcattttcg gagaaactaa acaggaaata cttagaattt
    59941 ttatctttta tctggcattt tgaaggaaac aatgaaccaa aaatacagca gctgatccaa
    60001 gtagctaatg caagaaataa actgatcatc cttaaaacaa gacgagagaa acaagaattt
    60061 ttaactaaat tgaaaataga gagcaaaaat aattgacagt ttttgtttac aagcgtaatc
    60121 tgtattcata agctaatgat tacatttgta aacgaaaggt gtgaaataaa tggaaaaagt
    60181 caaaacgcca gtaaagatca gtgattcaga gtgggagatc atgcgtgtta tttggacact
    60241 tggacagacg acagcccaag aaatcacgca gatcttagct gattcgatgg attggaaacc
    60301 agcaacgatc aaaactttgc tgggacgttt ggtaaaaaaa gaagtgatct ggacagaaca
    60361 aaaaggaaaa aaattcattt atcatccagc tgtctcggag atggaaacag ttcgcagtgc
    60421 gacggaaaat cttttttccc atatctgtgc aaaaaggatt ggagagacga ttgcggatct
    60481 agtagaagaa gc
//
LOCUS       DANKHJ010000010        59176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000010 DANKHJ010000000
VERSION     DANKHJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1087
                     /locus_tag="PW113_000721"
     CDS             <1..1087
                     /locus_tag="PW113_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2525054.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="PW113_000722"
     CDS             complement(1305..2447)
                     /locus_tag="PW113_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2525055.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="PW113_000723"
     CDS             complement(2452..2637)
                     /locus_tag="PW113_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2525056.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="PW113_000724"
     CDS             complement(2991..3236)
                     /locus_tag="PW113_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525057.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="PW113_000725"
     CDS             complement(3230..3631)
                     /locus_tag="PW113_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2525058.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PW113_000726"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PW113_000726"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2525059.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="PW113_000727"
     CDS             complement(5649..7415)
                     /locus_tag="PW113_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2525060.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="PW113_000728"
     CDS             complement(7428..8222)
                     /locus_tag="PW113_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2525061.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="PW113_000729"
     CDS             complement(8232..9608)
                     /locus_tag="PW113_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2525062.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="PW113_000730"
     CDS             complement(9728..10375)
                     /locus_tag="PW113_000730"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2525063.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="PW113_000731"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="PW113_000731"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2525064.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="PW113_000732"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="PW113_000732"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2525065.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="PW113_000733"
     CDS             complement(13042..14673)
                     /locus_tag="PW113_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2525066.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="PW113_000734"
     CDS             14977..15648
                     /locus_tag="PW113_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2525067.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="PW113_000735"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="PW113_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="PW113_000736"
     CDS             complement(16805..17257)
                     /locus_tag="PW113_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525068.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="PW113_000737"
     CDS             complement(17264..17812)
                     /locus_tag="PW113_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2525069.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="PW113_000738"
     CDS             complement(17857..18189)
                     /locus_tag="PW113_000738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525070.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18460)
                     /locus_tag="PW113_000739"
     CDS             complement(18203..18460)
                     /locus_tag="PW113_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525071.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="PW113_000740"
     CDS             complement(18655..19197)
                     /locus_tag="PW113_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525072.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="PW113_000741"
     CDS             complement(19218..20309)
                     /locus_tag="PW113_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2525073.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="PW113_000742"
     CDS             complement(20317..22527)
                     /locus_tag="PW113_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525074.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="PW113_000743"
     CDS             complement(22543..25071)
                     /locus_tag="PW113_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2525075.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="PW113_000744"
     CDS             complement(25123..25533)
                     /locus_tag="PW113_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525076.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="PW113_000745"
     CDS             complement(25546..25764)
                     /locus_tag="PW113_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525077.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26753)
                     /locus_tag="PW113_000746"
     CDS             complement(25761..26753)
                     /locus_tag="PW113_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2525078.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26832..27743)
                     /locus_tag="PW113_000747"
     CDS             complement(26832..27743)
                     /locus_tag="PW113_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2525079.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="PW113_000748"
     CDS             complement(27762..28106)
                     /locus_tag="PW113_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525080.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28682)
                     /locus_tag="PW113_000749"
                     /pseudo
     CDS             complement(28126..28682)
                     /locus_tag="PW113_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28763..29263)
                     /locus_tag="PW113_000750"
     CDS             complement(28763..29263)
                     /locus_tag="PW113_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525081.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="PW113_000751"
     CDS             complement(29264..29809)
                     /locus_tag="PW113_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525082.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="PW113_000752"
     CDS             complement(29806..31062)
                     /locus_tag="PW113_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2525083.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31422..31733)
                     /locus_tag="PW113_000753"
     CDS             complement(31422..31733)
                     /locus_tag="PW113_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525084.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31756..33066)
                     /locus_tag="PW113_000754"
     CDS             complement(31756..33066)
                     /locus_tag="PW113_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2525085.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33680..34132)
                     /locus_tag="PW113_000755"
     CDS             complement(33680..34132)
                     /locus_tag="PW113_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525086.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34134..34499)
                     /locus_tag="PW113_000756"
     CDS             complement(34134..34499)
                     /locus_tag="PW113_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525087.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34598..38881)
                     /locus_tag="PW113_000757"
     CDS             complement(34598..38881)
                     /locus_tag="PW113_000757"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2525088.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39028..39267)
                     /locus_tag="PW113_000758"
     CDS             complement(39028..39267)
                     /locus_tag="PW113_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525089.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39357..39629)
                     /locus_tag="PW113_000759"
     CDS             complement(39357..39629)
                     /locus_tag="PW113_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525090.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39712..39963)
                     /locus_tag="PW113_000760"
     CDS             complement(39712..39963)
                     /locus_tag="PW113_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525091.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40959..41870)
                     /locus_tag="PW113_000761"
     CDS             complement(40959..41870)
                     /locus_tag="PW113_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525092.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="PW113_000762"
     CDS             complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="PW113_000762"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2525093.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44048..45268
                     /locus_tag="PW113_000763"
     CDS             44048..45268
                     /locus_tag="PW113_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525094.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45288..45782
                     /locus_tag="PW113_000764"
     CDS             45288..45782
                     /locus_tag="PW113_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2525095.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45760..46446
                     /locus_tag="PW113_000765"
     CDS             45760..46446
                     /locus_tag="PW113_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525096.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="PW113_000766"
     CDS             complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="PW113_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2525097.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49457..50503)
                     /locus_tag="PW113_000767"
     CDS             complement(49457..50503)
                     /locus_tag="PW113_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2525098.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50514..50786)
                     /locus_tag="PW113_000768"
     CDS             complement(50514..50786)
                     /locus_tag="PW113_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2525099.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50821..51729)
                     /locus_tag="PW113_000769"
     CDS             complement(50821..51729)
                     /locus_tag="PW113_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2525100.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="PW113_000770"
     CDS             complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="PW113_000770"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2525101.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53108..54229)
                     /locus_tag="PW113_000771"
     CDS             complement(53108..54229)
                     /locus_tag="PW113_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2525102.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54226..54927)
                     /locus_tag="PW113_000772"
     CDS             complement(54226..54927)
                     /locus_tag="PW113_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2525103.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(55071..55511)
                     /locus_tag="PW113_000773"
     CDS             complement(55071..55511)
                     /locus_tag="PW113_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2525104.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(55640..56566)
                     /gene="dnaI"
                     /locus_tag="PW113_000774"
     CDS             complement(55640..56566)
                     /gene="dnaI"
                     /locus_tag="PW113_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2525105.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(56566..57939)
                     /locus_tag="PW113_000775"
     CDS             complement(56566..57939)
                     /locus_tag="PW113_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2525106.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(57962..58453)
                     /gene="nrdR"
                     /locus_tag="PW113_000776"
     CDS             complement(57962..58453)
                     /gene="nrdR"
                     /locus_tag="PW113_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2525107.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(58765..59097)
                     /locus_tag="PW113_000777"
     CDS             complement(58765..59097)
                     /locus_tag="PW113_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2525108.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT        17625 a        12289 c         8942 g        20320 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gacctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctgaaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcaaga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt ttaacccttt agaaacaccg atatatcaac tagtccccct
    31141 cgttttttgg gaaaagtagt acaactgctg accttagata atcacttgat acaaaagggt
    31201 ttataaaact tttttctatg tgttatgtta tttctgcccc ccatattata tatcgggggg
    31261 ctttacacaa agaggccgtg cgcaagcgca cgacctcttt gtgagctgca cacgctgcta
    31321 gcgagtgcgc attataggcc catgcttcgc agccttaatg cgcaccgcca ggcagagtgc
    31381 ggctaccaga ctacgtctag agccctcccg atagataata actacaaaat taaatgatct
    31441 aattttgtta tttcactata tttttcagga gaaaaaacgc gataaattga caaagttaaa
    31501 tcctcttcta aatctactcg ataatgatac aatcgagtca atcgttctat atcttttttc
    31561 gttagcaata cttgattaat aacttttcct gtaaaaacac gttctttgcg caaaaagcgg
    31621 taatcagtta cttgttcaat acgattcaac caatcataaa acgttctgga agaaagagaa
    31681 ggattaatca ttcgtattgc tgtagctact ctataataac aactctctgc catttaactt
    31741 cacctcctta gttgattaca aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt
    31801 tgctaaatac atttggcgct ctagttgttc ttgtaacaac tgattatcat ctcccatcgt
    31861 ttgatcttga ctatcctctt ttactgtaaa atcatagaca atcatttcag taaatttctc
    31921 aaattctttg ataaagtgat aatctttgta aacaaatggc gcaaaaaact ctacaggtac
    31981 atcaaatcct ggaaaagcat atccacgacc aggttgatct ttgttgtaaa atggcttatt
    32041 tttaaaatct tctccaaaag tcattaaata accaatggga gacaatcgac caacagaaat
    32101 acggaatgtt aaattatccc gaacaccact tggaaaatat tctgcatctg gtcgttgcat
    32161 tgctaaaata atgtacatcc ctgcttgacg accctttaat actaatggct gaatcaattt
    32221 atcgactcta tttctcgctt ccatatccaa cgaaccataa aaagctgccc attcatcaaa
    32281 aaagataaaa tgcggtggta acccataata agcataattt ttccctggtt catatttagg
    32341 atctttcatg gttttcatat aggcataccg ttgattcatt tcttttaatg catttcgtaa
    32401 acaaataatc atttcttcat ttattttgta aaacacatgt cctttgaata catcgacgtc
    32461 acctaatgct gctaaatctg ccgcttttcc atcgcaaata tctactgtcc caacacttaa
    32521 acaagcttga attaaagaat acaataaata ggttttccca ccaccgattg caccagcaat
    32581 caacatattt ggtgattgat catataccca atccaaagtt ttcattaaat gaatacgtcc
    32641 attttctgct atacattgat caataggaat ccgaccagaa tctgaaagga atacataatt
    32701 gattcgggct ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cagataacaa
    32761 atgacccatt tttaaaaatc gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac
    32821 taattcttgt ttcttttgat aaaaataaac ttttggtaat ctcatttttt cccttgtttg
    32881 tttctcacta acttgtttct ttgtataaac aaaccgattg tcaattaaca ttcgaacaag
    32941 taactgacgc caataaacag aggcaaaata tccttctttt agtcgcgacc aatgcatcaa
    33001 ccctaaaatt aacccactaa ccagcaacaa atacaacaca atacttattc caggaataag
    33061 atacatttgc caattcgaaa acgtacttaa acgtggataa aatactttcc aactaaacca
    33121 acctaacaga atcaaaaatg gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg
    33181 attgaaatag cgaattcgcc gaccacgata cttgaaaaaa tgagctttca attgtcacat
    33241 tcctcctttc tgtttttact tttcctgtgc taaaaacttt tgacaaaaag caactaatac
    33301 aggaatatca catttagtaa aaaaacataa tcgaaaaaga tttaatcgat ttttataagg
    33361 gtgtgcacac atattacata ttcgtactat acgaatccaa atcacttcct gatgatcttg
    33421 tttccactgg tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat
    33481 aaccgcacaa atatatgcat ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc
    33541 aatcaattgt tggatatttt gataacaagc acaatctctc aaagtatttg attggtttta
    33601 tctgaattca aaatctcccc ttccttctat cttagataaa aagaatagga agactccttc
    33661 ctattctttc aaaaaaacct tattttttct ctttttcctt agaatcaaca cttggttgtt
    33721 tttgatctaa acctttttct tgtgattgtt tcatattagg attatttgtt ggagcttcta
    33781 ctttctctaa tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg
    33841 ctggaatata taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa
    33901 aaccagtcaa atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg
    33961 gatttccaaa aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt
    34021 cattatcaaa tacacgatca gactcttcta atgcgccaaa gcgtacggct cctaaacaag
    34081 tttcatctaa tgcttctgta atgtcataat tatttggaaa ttttaatccc atcttacaaa
    34141 acctccccaa tttttacacc agttaaaaag acaccaacat cttgatagcc agttgttcga
    34201 cgctctgttt gtcccatatc atttgtttca cgactattca ctcgttcttt acgaaaaaca
    34261 aaataagctt cttcaaatga aatacgcacc tctttttctg gtaatcgtaa tcttttatcc
    34321 tcttcagctg tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt
    34381 aattgaacat atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa
    34441 cccaataaaa atacttgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata
    34501 aagtttttcc tcctcttaaa aaccaaaata aattctaaat atttctacat tacaatcaaa
    34561 caagcggtta aaaatcctaa ccgcttgttc ctcatgacta atctttggct ttacgtactt
    34621 tataaagtgt tatacctaaa gcaatgacta atactactaa tccaagaacc ataagtatac
    34681 tacttgtttg ttcacctgtt tttggcaaca ttggtacaac agtagctggt ttaacccgtt
    34741 gttctggttt cactggttgc ggtggttttg gcgcttccac aatttgaacc gtttgatctt
    34801 tgtttgttag atctacattg tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg
    34861 ttttcgcttg gagccattcc gcaaaactca agccttttcc tgcaagattg cgaccgtcca
    34921 ctaagaattc aaataagaat tccatcattt ctttatcggc cgcaaagact ttttccgcat
    34981 gagtgccttc aaatggtgtg ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga
    35041 tttgaccaat cgcaagaccg tctactttga cccattcaag cacagtttga tttggcaagg
    35101 ccaagatctc ttttccgcca tttttattcg tcgctttcgt cgtaatggtt ggattactga
    35161 ttttgatggt ttggtcattg tcggtaatat ctgcgtgaga agccaataat acgccttctc
    35221 taaacagatc ttcaaagacc acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa
    35281 agtctagatt gactgtacca tccgttgttt ctgccacaaa cgttaaggta cttgtgactt
    35341 cttttccatc tactagcact ggtttttccg ttactttgtc cattagaaca ccgttgactg
    35401 tgtatgtttt tccaacgacc aaatcgtggt atttcacagc gtctgtaatg gtgactttcg
    35461 ttaatggatt aacctctttc aagccagtgg gtttatgtgt agccgttgta cgtacacttg
    35521 ggttagttac tcgtaccgtt tgccctttgt cgttgatgtc tttatggttt gctagttctt
    35581 catcatttct aaaaagactt tcaaacacga cgatttcacg accatttata cctgtcaaat
    35641 caaaggtaaa tggtacgatc accgtttcat tttctgcttt ggcaataaag ccaacactcg
    35701 tttctaccgt atttccatgc acaagcaagg gttcaccggt tgctttatcc attaagatcc
    35761 ctttgaccaa gtatgctttt cctggaagaa tctcggtaat ttcaacggta tcgtttaagg
    35821 taaacaatcc tgttgggtct gcgactgtca atccgtcttc aaagacagcc gtggtatgaa
    35881 cggttggatt attcactttc acactttggg cttcatcgtt gatatccgca tgtgctgcaa
    35941 cttcaatgcc atcacggaaa agtttttcaa aaacaacgat cgtttgacct ttcagaacac
    36001 tagcattaac cacaaattca aggtcttttg tgccattgac tttgtctgca ataaaggcca
    36061 aggtacttgt gatttctttg ccatcttgta aaattggcgc attcgttgcc ttgttcatca
    36121 atactcctga aatggtgtat tcttttccga caaacaagtt ggtataagaa acactgtctt
    36181 taatgacgat cgtttctaat ggatcaacca ctttttcacc atcggcttta tttgacgctt
    36241 gtgtttctag ttttggtgga atcaagtaat tacgtacaga aaccactaac gtttccccat
    36301 catctggcgc attttgtttg aatgcaaccg tgaatgcttg tttatctgta tttggttgga
    36361 aactttctaa tggggcattt tccaagaagt aatagtcatc ggcaactaga cctttcacta
    36421 aaatttcccc gttttgatca gtggtatatt caccaattgc ttgattttta ccgttataca
    36481 aggtaaagtt cacacctgcg agcggcgttt tcttcttgtt ccattcttca tacttgaata
    36541 attttacact tttccgtgct tgacggttcg caaaatggat cactttgatc ggttctgtat
    36601 cattggtaat cgagaacact aatgggtttt gattcaaaac gtaatttacg ggggctttga
    36661 tttcttccaa acgatagcga tcttttccat aagtaagtgt accgttagtt aggaaaaagc
    36721 ctttttcatt ggttttaaag atatccgttg tttctgtatc atttggaatc tccattgtga
    36781 cataacgatt ggctaaagta tcaaaaatct tgaattctgt attagcaagt ggaatcactt
    36841 ttcctgtttc ttgatccact ttcaccactt taagacgttg ttcaattact ttatcttcta
    36901 aaacataatg gaaagtttgc ccattttcag aaattgtgac ttcaagtggt tcaaatagtt
    36961 tatacccttc ttttcctttg gtttcagaaa gaatgtacgt atcaaagaca acggtatttt
    37021 taccaaaagt agcataacca tcattgtctg tcacaacggt tttcaccact ttgccagttg
    37081 tttttgacgt caaagtgatt tcaatgccag ctaattttgg tttgttatct ggattcgttg
    37141 aatcgactaa atctttatta gcaactttca ctaaatcaat ggttcctttg atcacttgat
    37201 cttttgcggt cgcattgaca gtttttccag gttcaacaac aatttcaatt gcttcatcag
    37261 aaagcacata accttttggc gctttgattt cttttaaaac atatttgcca ggatcaagat
    37321 ctttaatttc tgcctgtact ttgccgttaa tttttttcaa ggtaatttct ttaatctttt
    37381 tcccatctgc ttgatacaaa ccatagaccg cattttctaa agaagctgca ccttgtgctt
    37441 tcacaccagt ttccgcatct tctttaaaca cacgaatgtt tccttgtttt agaatttgta
    37501 aattaataac agacgcatta ggttctgttg gatgtaacac accaattgtt tgagctcctg
    37561 gacgacgata ggctaatggc gcaccttgaa ctttaatttt attcaattga atttttgctt
    37621 tatccggtgc ctcttttgtg actgtaatcc atacttcatt gccgtgaata gatacattga
    37681 caccttttgg tttttgaatg tctttcacat atttcaaaaa atcattttta ttctttactt
    37741 tcaacgtttc gccaattttt accgtatgtg tttgattatt aaaagttgct ttttcaccaa
    37801 agttattaat caaacgttga atttctgctt ttcgacgatc ataatcaaca cggatatttt
    37861 tgattgggat ttttgtgccc aaagcttccc aaatcataaa ttgtgccgca atgtattgtt
    37921 catcggattt gtctttattt gtgacatatc ctaaataaga gatcaagctt gtttttttcc
    37981 gtacatttga atttttcaaa tagtttttca aagcagtagc tgtataaact tgaccttctg
    38041 cggctgagac accaggctca atacaaaacg ccacttgtcc attaatctca aaacgaacca
    38101 tataatttaa agtgaagttt agattgttcg gactagaagt aacaccaata ttttcgcccc
    38161 aatcaatttt ccgaccacct aaaacagctg cattggttgc acggcgaaca cgaaccgaac
    38221 gattggttgg tactgttact aacagtgtag gtgtacctgt ttttggaatg ttctgctctt
    38281 ttttcttttg attttcaata ctagccgtag attctttgtt tggtttcgtt ggttgatttt
    38341 gactagctgc ggttgaagag attgtttttt caacgttttc ctcatttttt ccttctttgt
    38401 cagcagtagc cgctggttgt tccactgttt cttctattcg tttcttttct tccgcttgtt
    38461 tctcagaaga ctctggctgc acagaaggcg ttgcttcggc agctttttct ccttctagag
    38521 tggacgtttc tgaagcaggg gttgtaattg aggcagtttc ttcaactggt tgtgaagtac
    38581 caggactatt ccctgggtgt tgtgtttccc caatcgtctg atcgagagtc gttttggaat
    38641 tgaccgttgg cgcttctgtc acaggggcct gttcaacagt ggcttcacca gctgggaccc
    38701 cggcttctgc tggttggact tctgtcactt cagaagcgac ttgggcaatg gcttccatcg
    38761 taggttgtat agccgtcgtt tcttctgcat gtgcttgagc agttcccacc aagaaacaga
    38821 attgagaaaa agccaccaat gcgagtgctt tcaaccaatt cattttcgtt tttgcataca
    38881 caagaattcc tccttttcct gagaatttta tataataaaa caaaacgccg ttaccaaaag
    38941 tgataacgac gtgaactgtc tcttctattt ttatctggcg ctcttatctg aacacatttc
    39001 atcaatgatt ttaccctccc actataatta cagaacaaaa aaagaacctg cttcgtggaa
    39061 atcaactgtc catttcccaa ttggaatacc agcttttttg gcaacttcac catcaccaaa
    39121 tctcatgcca taccaattac taccttcatc cttaatttct ttccaaccat atgacatagc
    39181 ttcatctaat gaatcaaaaa tcatgacact attgccaatt gttacaggat ctggcttaag
    39241 atcttctgga agagaaatat caatcatacc ataatgatcc gattcctcag tattattgtc
    39301 attactagct actggtttgg ttgttctact ggcttatttt gatacaacaa gtactcttat
    39361 tcttctttta cttctccgtt ttcaatttcc cattcaaacc actccgcaaa gtcttgctta
    39421 aaaccatcta ctcgatacct attttgtaaa taggcatctt ctgacgtcgt aggatctaaa
    39481 taggtaggtt tattctctgg acgagcatag acacctaccc atgtttcgta atctccttca
    39541 aaataactat ctaccaaata gccttttttt ctaagaatct cccgaacttg atttttaatt
    39601 attgatactt gttcaaaacc atcactcatt ctatcacctc aaataactct agattctcat
    39661 aaatattccc gacaatttca atcctatcaa tgatctctaa caaatcgcta cttatgcttc
    39721 cccattcgta taagaatttc ccttccgcaa aataaatttt ccataaactt cttcatgatc
    39781 gtcccaacca atatcactct cgaaaaattc cacgttgttc ttatctttta gctcagtaaa
    39841 tgccataagt tcaacatgtt ttccaacctc gaaacaatca caccacccat caaccgttac
    39901 acgtgtgcct tcaataccta agaaactttg tcctcgtgta ttaaacacta aaactttccg
    39961 cattttttgc tcattaatta atcacgcgcg aaatctagga atcacaactt attcgcttct
    40021 tctttataaa taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt
    40081 taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa
    40141 actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct
    40201 tttactagaa cttgtataca aaaattgttg aatgctgata taatttgcgg ctacttgaaa
    40261 aaacgataga tgtaaaaaga taaatagaat cattttttat tctatatcag tctatgattc
    40321 attgcttatc aatcacccaa cccattcgat gccgtttttt ttagagaaat ttaaaaaaga
    40381 ccgtacgaat ctcgcattat ccgattactt ttccagaaaa gatccccaat tctgtataaa
    40441 taaccctatc ttcttgttgg tttcattttc tcatataaga attcagtcaa tctacccact
    40501 atgaccatta ctacgtccaa cacaactgac atattaacca cgtaagtatt ccctcacaga
    40561 tctccgaatc tccatggtat ggattcccat cctgacgctg cagaggacaa catatttcat
    40621 gtggctctgt acccttttga tttggaaaca ctacaaatca atactcatct ataacttatc
    40681 aagcgaactt tcgctaggct tctagtgatt actaaattgt tggataacat acatagccca
    40741 ttttatctag ctgttattca taacagttag ttcttgtatt acttcttccg cttgtgttgc
    40801 cattgagctt gtcaaaaagt tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa
    40861 ccctttttgt ttcttatcat atcaagaaac ctccttttat aaattttttc tataataaaa
    40921 aacgtcgata acttaataag tatcgacgta aactattttt atatcccgta aatcaaaaat
    40981 tctaaagaaa gatttcctat ataagcatat tggataattc gattagtatt gggtatatct
    41041 ctaccattct catacctatc tatggaccat tttgaaattg aaggagaaaa aagctttcca
    41101 aactcctccc tttctagata ataatctttc ctaattttcc tcatccttaa tcccatacta
    41161 aaagaattta tctttttaaa atcgctaact ctttcatctt ttggaagtat attcttataa
    41221 tcttctccgt acataaggta aggaacagtg accttaccaa gataggcaat attcattagt
    41281 ttttcaatat ctggaatatt aatacctttt tcccatctac ttataacatt tttgcctgta
    41341 tagatggtaa agtttttacc aaatttttca agcgtctctt cccggttctt tcggacttct
    41401 cgtattcttt ttcccataac aattttatcg tcttttgata aactaaatat ctcactatca
    41461 cgattaatct ccttcaaaat ttctttttca ttacttaata gaaaaatatc gatttttttt
    41521 tgcatttctt ttgacggatt tctctttcta tttaacacat aactgagtaa tacttcacta
    41581 attcctatat tatcagctaa ctctttgtta agtaacttat tcatatctct gtatgcaatt
    41641 agtttaccga tgagaatctc aatcttatct tcctcatctt ttattagagt ttctatattc
    41701 agaatcttgc ttagagtatt atctacgtct ttagggattt ttcttgaccc tttttcatat
    41761 ttaactaaag tactttgtga aatacctgct aaatcagcta actttttttg agatagtttt
    41821 ttctcttttc ttagagattt aagaaccctt ccacttgaat tatcaaccat gaaaaaagca
    41881 cctcctatga gatgctttca tcatatactg acagctaaaa atcttcaaga aaaatattcc
    41941 aattttgtta attttagtat cgccgtgata accaaatgac agtttaaaat cttttataca
    42001 gataatcctc agtgttgcat ctactcaaaa aggtttattt aataggtttt tcgattgatt
    42061 ataagttatt tcacactatc ttgctcatcc attttcagaa cggccatgaa ggcttcttgc
    42121 ggaacttcga cggagccgat ttgtttcatc cgcttcttcc cttctttttg tttctctaag
    42181 agtttacgtt tacgagaaac atctccacca taacatttag ccaagacgtt tttacgcaag
    42241 gctttgatgt ctgaacgggc aacgattttt tgtccaatag ctgcttggat tggcacctca
    42301 aattgttggc gtgggattag ttttttcagt ttttcaacga ttgcttttcc acgttcatag
    42361 gcaaagtctc ggtgaacgat aaagcttaat gcatcgactt tttctccatt cagcaagatg
    42421 tccatcttca ctaacttgct tttttgataa ccagacattt cataatccaa tgaggcatag
    42481 cctttcgtgc ttgatttcaa tttgtcgaaa aagtcaaaga caatttcaga aagcggaata
    42541 ttatagacga catttactcg gtaatcatct aaataatcca tcgtaatgaa ttctccccgt
    42601 tttcgttgag aaagttccat tactgcaccg acaaaatcat ttgggaccat gatctgtgct
    42661 ttgacgaatg gttcttctac atcctgaatc gttacaggtt caggaaaatc tgccgggtta
    42721 tctactgtag cagtcgtacc atccgtttta ttaacatggt aaataacgga tggtgcagtc
    42781 gtgatcaatt ctagattgaa ttcccgttcc aagcgttctt ggacgacatc catatgaaga
    42841 agtccgagga atccacagcg gaacccaaaa cctaatgctt gagaggtttc tggttcgaat
    42901 tgtaaagcag catcattcaa ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga
    42961 gaagtatcaa ttggataaag accacaataa accatcggat tcatttttcg gtaacctggt
    43021 aaagcttcag cggcaggatt gtctgctaac gttactgtat cccctactcg tgtatcttgg
    43081 acagttttga tacttgctgt gatataaccg acatcaccga ccatcaaaaa gtctctagca
    43141 actgcttttg gcgagaagac cccgacctct gtaacatcaa atgttttgcc attactcatc
    43201 agtttgattt tgtctccggg tttgaccata ccgtccgtga tccggacatt tagaacaaca
    43261 ccgcggtaac tatcataaat agagtcaaag atcaaagctt ttagcggtgc atctaagtct
    43321 ccacttggtg caggaacata ttccacgatc tgctcgagaa tatcttcaat cccaatccct
    43381 gatttggcac tggctaatac agcttcacta gcatcgattc cgattacgtc ttcgatttct
    43441 tgacggacac gttcgggatc tgctgcgggt aggtcgattt tattgatgac tggcaggatc
    43501 tccaaatcat tgtctaacgc taaataaaca ttggccaatg tttgtgcttc gattccttgt
    43561 gctgcatcga ccactaaaac ggcaccctca caggcagcaa ggcttcgaga gacttcatag
    43621 gtgaagtcga cgtgccctgg ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc
    43681 gctgtgtaat tgagttctac agcatttaat ttaatcgtga ttccacgttc acgttccaaa
    43741 tccatcgagt ctaacagctg atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct
    43801 aaaatacgat cggccaatgt agatttccca tggtcgatat gagcgatgat cgaaaaatta
    43861 cgaatctgct cttggcgttc tttcattttc tttatatcca tagcgtctcc tcttttcata
    43921 taatcagcac tttccattat accaacgaaa agcagtaatt taaagagttt ttaattagtc
    43981 agctatagaa gttttttttg tagaattttg ctatactgtt atcaagctaa taaatggaga
    44041 tgaaaaaatg gatcaaaaag attttagaga aaatcttgaa ttaaaaccgg tcactcaaga
    44101 acatatcgat caattcaatg aattgctttc ctatgtgttc caagtgactg aagcagacat
    44161 cgaggaaagc gggtttgaga ataaacgagc ttttatccgt tccaaaaaac cgattttgga
    44221 agtatcgaaa gtgttcggat ggttccacga aaataaactg atctctcaaa tcgctatcta
    44281 tccttgtgaa gtcaatattc acggtgcacg ttataaaatg ggtggtgtga ctggagttgg
    44341 aacttatcca gaatatgcta accatggatt gatgcaagat ctaattatcg ttgcattgga
    44401 taatatgcgc aagaataagc aatggatctc ttatttgtat ccttacagta tcccctacta
    44461 tcggcgtaaa ggatgggaaa tcatgtctga taaactttcc tttaaaatca gagatacaca
    44521 gctacctaaa caaatcccag taagtggtat ggttgagcga ttaccagttg atcatccaga
    44581 cgtattttcc gtttatgaca aatttgccca acaaaaccat ggtgctttat tccgcagtga
    44641 ttttcattgg gaagagtatt ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta
    44701 ctacgatagc aaccatgaac caagaggtgt tctgttctac tgggtagctg aagaaatatt
    44761 tcacgtaaaa gaaatgttct atcttgatca agaagctcga aatggcttat ggaactttat
    44821 ttccgcacat ttttccatga tctactgggt acatggagat atttataaaa atgagccttt
    44881 agcatttttg attgaagata gtcagatcaa agagcagatc gaaccatatt ttatggctcg
    44941 gatcgttgat gtaaaagaat tccttcaacg gtttccgttt gttggaacag ctgatgcttt
    45001 tcatttcatc attgaagatc ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg
    45061 ggatgaacag ggacaagtcc gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga
    45121 tatccaaaca ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat
    45181 cgaacggtta gaaacagatg aagaaacact gaagtcacta gagcgtatca ttccaaacat
    45241 ggaagcttac tttagcgatt atttttgata aaaaggatga tgtttagatg aaaatttatt
    45301 ttgccgcacc aatgtttgct aaaagtgatt tgttatataa ccaacagtta gttaaagaaa
    45361 tcagagagct ctcttctgag ctttcgatct atcttcctca agaaaatgaa gcaatcaatg
    45421 acaaaactgc ttatgcagac agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg
    45481 ctagtgattt aatgattgct ttactagatg ggctgacgat cgatgccggg gtagcatcgg
    45541 aaatcggtgt ggcttatgcg aaaggcatcc cagtgatcgg cttatatact gatactcgac
    45601 agcaaggcgg aacccacccg aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat
    45661 ttcactacct gaacttatat acaataggtt tgatcaaatt gaacggaaaa gtggtttctt
    45721 cagaaattga gttgctttca gaagtaaaaa gatttttaga tggagggact ttcagtgatt
    45781 aaagaaatca aaaaggtgca aatagcttta cttgcttttg gtgtattcgt tgtctgctat
    45841 aatttttatg aatttatcac acaaaaatat tcgacttctc aaggaatcac atttatcgtg
    45901 gaatctttat tagggattgc attgattttc atgccacaag tcattttgaa agttttcaaa
    45961 cttaaaatac cagcagcaat cgttttgttt tactggtttt tcttgttcat ctctgtcttt
    46021 ttaggcacag ggatgcattt gatcagtatc atctcttttt gggacaaaat cttacatgcg
    46081 gtgagtccaa tggtcctgac agctcttggc tatggattga ttggttactt gatgaaagat
    46141 gctgagatat caaaaaccag tccttggttg ttcctcttat tcggctttgc gtttgctgga
    46201 ctttgcggcg tgttttggga gttctgggaa ttcttatgcg accagttctt aggcatgaat
    46261 cttcaacgtt ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc attgatggat
    46321 acgatgggcg acttgctgac aaatacgata ggtgctgcct taatgggatt gtttgcatgg
    46381 tctcaaagta aaaaagatga gcgttacttt gaaagttata aattagaaaa agtgaaaaat
    46441 acctaataaa agaggttgtg acagaagcgg gcagcttcaa gaaatttcgg cttatttttc
    46501 gaagaaacta cttctggaac actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt
    46561 cagacccctt ttttctttta tagtctcaat ggaagattag acgatttctt ctagctcttc
    46621 tgttttgaaa tgaagttgtc catcaagtaa agtaatcgtt acttttgatt taggcataac
    46681 atgtcctgca acgatttctt tagctagtgg tgtttctact tccttagtga taaatcgttt
    46741 caatggtctt gcaccatatg ctggttcata agcattttca gcaatccacg ttttggcttc
    46801 atcactgatt gtcagcagga tttcttgatg ttccagacgt tgtgccaatt gcgccaccat
    46861 tttgtcaacg attcctttca cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc
    46921 gattcggttc aagaattctg gtttgaagtt tccacgtaat agcgtgttga cttgttcagc
    46981 cactgcttct ggaatcgttc catcagcagt cacgccttcc agcagtaact gtgaaccgat
    47041 attgctggtc atgatcagaa ccgtattttt gaaatctact actcgccctt ttgaatcagt
    47101 caagcgtccg tcatctagga cttgtaacaa gatattgaag acatcgggat gcgctttttc
    47161 gatttcatcc aacaagacga ttgtataagg atttcgtctg acagcttccg ttaattgacc
    47221 accttcttcg taacctacat agcctggagg agctccgact agacgagaca cagcatgttt
    47281 ttccatatat tcactcatat cgatccgtac catatgatct tctgagtcaa acaagttctc
    47341 agctaacgct ttcgctaatt cggtttttcc gacaccagtt ggtcctagga aaaggaaaga
    47401 acctagagga cgatttgggt cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac
    47461 cgcatcaact gcttcatctt gaccaatcac tcgtttatga agtgtttcat tcaatttaat
    47521 tagtttttct cgttcacctt ctacaagttt cgttacgggg atacccgtta atctgccaac
    47581 gacttgagca atctcatttt cggtaacgga ctcttgaacc atcttaatct cgctgtcttt
    47641 tgctttagct tctaattcct tcaattcttt ttctaattga ggtatcgtac catgacgcaa
    47701 tacagcagcg cgttctaaat catagttatt ttctgcatct tctaattcgt gttttgcttt
    47761 atcgatttca gcacgttttg ctgaaacaga attgacttct tctttttctg tttcccactg
    47821 catcttcatc gcatttgctt cttcacgaag ttcagctaat tcttcttgca aatttttcaa
    47881 gcgtttttta cttgcatcat ctgattcttt tttcagtgca gcttcttcga tttccaactg
    47941 catcaaacgt ctagtaactt gatctaattc cgttggcata gagttcattt ctacccgaat
    48001 cgttgcactg gcttcatcga tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat
    48061 ataacggtca gataaagtag ctgctgctac taatgcgttg tcatggatgt taacaccatg
    48121 gtggatctca aaacgttctt ttaatccacg cagaatactg atcgtatctt ccactgttgg
    48181 ttctttgact aatacctttt gaaagcgacg ttccaacgct ttgtcttttt ccatatattg
    48241 acggtactca tctaaagttg tggcaccgat caaatgtaat tcaccgcgag caagcattgg
    48301 tttcaacaga tttccggcat ccatacttcc ttctgttttt cctgccccta cgatattgtg
    48361 gatttcatcg atgaacaaga ggatacgtcc atcacttttt ttgacttctt tcaagactgc
    48421 ttttagtctt tcttcaaatt caccacggaa ttttgctcca gcgatcaatg cacccatatc
    48481 taatgaaaaa atcgttttat cttttaaatt ttcaggcaca tctttgcgta cgatacgttg
    48541 agcaagccct tcaacgattg ccgttttccc tacaccgggt tctcctatca gaacaggatt
    48601 attttttgtt tttcgtgaaa gaatacggat gacatcacgg atttcttcat cacgaccgat
    48661 gattggatcc atttttccgc ttttgacttg ctgtactaaa tcaacgccgt atttttctaa
    48721 tgctttgtat tgttcttctt ggttttgtga ggtcactctt tctcctcctc tcatctcttc
    48781 gatgttttta cgcaattctt tttcagataa tccgttttta ttcaaataaa cagttaacgg
    48841 ataatttttt aatttcatca acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc
    48901 gaagctttca cgtatttggt ctgcttcatt caataatcga aagaaattct ggctgaggtt
    48961 ttgtccatat tggacgtttc caccagaaac aactggatac tcatccagta gacgatcaat
    49021 ttcatgttca aaagattcga catccaatcc ggcatccgta tagaaatttc gaccaaaatg
    49081 attagattgt aaaaagattt tccatacatg ggcaatgtcg atatcttgat gttttcgagt
    49141 gaccgcaatc tgttgagctt cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat
    49201 attcatacag gtataccctc ctaattaaag gtaagatcac ttatttcttt gatctttaca
    49261 aaaactatta tatcgctttg gtcaattttg gtcaaaatat acacatagtt ttttgacttt
    49321 ttttctcgac ttatataaat cttaaaaaaa caaaattgat tgtatgtata taaaaaaaga
    49381 gccaaacaaa cgtaaaatta tcttatccat aacgtttttc tttgatttga taaaaagggt
    49441 agatcttata aaagacctac ccttgatttt ctttattctc tttttttact tctattattt
    49501 cttctttgat cactcttcgc cgttcagcaa tcacatacac aaatccatag agaaatggca
    49561 agataaagga actgaaacga tacagtaaaa gcgaagaaac tgcgtcaact gttccaacaa
    49621 ctggtctgaa taataacaaa tagacaaatt caaaggaacc aataccagca ggcgtaggaa
    49681 taacccctga aagcactact gcaaagctga taaacgaaat ggtcagtaga aaatctattt
    49741 taggatggtt ttcaatcaga atcaaataag gaagtacata ccaaaataca agttttaaca
    49801 tattccatag ataaatccgc aatagcgcag tcctgtcttg gataatggtt tggaccgttt
    49861 cccttaaaga gtagatctgc tgattgcatg tatccaccca tttccgcaaa cgctcacttt
    49921 taaataactt attcgataag ctgacaaaaa atatttgaac attcaagctg acggacatcg
    49981 ccaataaaaa ggcaacaatc acgatcgtca aaaggatacc agcgatgata aaccagatca
    50041 ttgatggacc attagaataa aaaagagaga attggatgac caagccagct aatgcataag
    50101 tgatcactgc cagtttgtac ataatcatat ggagtgctgt cacccctgct ccctgggata
    50161 gcttcattcc ttttttgtta taaaaataaa tctccgagat cagcgttcct gtaccaaacg
    50221 aaacgattcg gtaaaacgca atatagctag aggtcaagaa cccatctttc gttgtaaaat
    50281 tcggcgcgaa aggttcagca atttctttga ttgagcgccc ttcagcgatt tggtaaactg
    50341 tccctaatat aacagccgct gccaaaacaa caacactggt agacagcaat tcggcaaaga
    50401 tatctgcaaa tgattgattg attaaataat aaataatggc aaaaatcaca atcagcaaaa
    50461 gcagattcag aatgatttta gttttcgttc ctttagacga catctcctgc tccttaatgg
    50521 aaaaaataaa tgagaatcaa atcaacaagt acacctgcag cgaatcctat agtaaacttc
    50581 tttttccgag ttttatgatg gaaaactttt cgagagatca atcctcccaa accaccgcct
    50641 aacaggctga caaatatcaa attattctca ggaacacgcc agccgctagt tctagcctga
    50701 tgtttatcat accccatcag acaaaataag taaaaattca caatgaaaag gtaaaaccac
    50761 aaaggattgt gtaacaatac atccattact cttcttccaa ctttctgtta actactattt
    50821 ctaagctttt tcttctttct ttggtgaata aactgttaat ccttttgata caacagataa
    50881 ttcaactgga agatcatctg tcgtgtctcc atccgttcga gtgcccacag atttttcctg
    50941 cgcttggatt ttcatgcgtt tggctgtgaa ataagtaatg ccggggatcg tatagaattt
    51001 gcctctcatg attctaggca aataaaaaat aaatcggaaa atatctaagg aaggtacgat
    51061 cgttacatga aattttccat catctagggt cgctgagtcg tcaaaatttg taaaaccgcc
    51121 cacggagtta gacatcgtga ctgtcaacag ttgggatttt cctttccaat gctcctcgtc
    51181 tgtttctaaa tccaaatgat attttttctt cttcagcaat aacttaaaaa acttcttgat
    51241 aaagatccac gagccgtaac gctgtttttc ttcttgactg atccaaactg ctgtatctgc
    51301 taagattccg attgtcagtg tagagataat gacttcatca ttgacttgac catagtctat
    51361 tttccgtaca ttgccttcca ggatcacgtc agcagcttct tcttttttga ggggaatctc
    51421 aagaacacgt gcaaggttgt tgacagttcc acctggaagt aatccgacat ggtattgaga
    51481 taatgttttt tggaacgctt ggacgatatg atgaatcgtg ccgtctccac ctataattac
    51541 taaagtatca gcttctatgg cttctgcttt cttgactaat tccgcatcat caatcgatgg
    51601 gccagtttca gctagttcta catataattc tggctgcttt tcttttacat aatcttgaaa
    51661 ccagcgtgct aatttctcac cctcatcttt ccctgaactt ttgttataaa aaactagcag
    51721 cttttccatt ctttacctcc tatttactct tcaaaaaaga gggcgggaca accaattcca
    51781 ttgtcctgcc ctttagcctt agacagatga cattccatca ttgggacgaa cgccttgtca
    51841 taagctgttc tttttcgatt ttaactcttt gtgttcaatt ctgcaatttt tacaatcacg
    51901 tctgttgctt tttccattac ttgtaaagaa acaaattcga atcgtccgtg catattttct
    51961 cctccggcaa atagatttgg cgtcggaata cccatgtaag agatttttga accatctgtt
    52021 cctccacgaa ctggttcgat cactggagag ataccaagct cgatcattgc ttgtttcgct
    52081 aattccacga tgctcatatc tttttcaatg atttctttca tgttgtaata ttgatcatac
    52141 atatctactt tgattcgttc ttgatcaaat cgctgattca actcttcttg gatatgtgtg
    52201 atttgtgctt tgcgtgcttc gaatttttca cgatcatgat cgcgaatgat ataactcata
    52261 gaagcttctt caggattccc atccaatgcc atcaaatgga agaaaccttc gtatccttcc
    52321 gttttttctg gaacttcatc ggctggaagc tgattatgaa aatcgattgc taactgtaga
    52381 gcattgatca ttgtatcttt tgccgtccct ggatgtacat ttttcccttg gatcgtaata
    52441 tttgcttgag cagcactaaa cgtttcatac tgcaactcac ctaaaggacc accatcgact
    52501 gtataagcaa aatctacatc gaattgctca acatcgaatt tatccgctcc gacaccgatt
    52561 tcttcatcag gaccaaaagc cactttgatg gtaccgtgag ggatagaagg attattgatc
    52621 aagatctcca ttgcagtcat gatttccgca atccctgatt tgtcatctgc tcccagtaat
    52681 gtactgccat ctgttgtgat caatgtgtgg ccttcataat ttttcaaatt tggaaaatct
    52741 tttgtattca atgtatagcg ttcttccgca tctagtacga tcgtagactc gccatcatag
    52801 ttttcaatga tttgtggatt gactcctact gcattaaaat ctgctgtatc catgtgggaa
    52861 ataaatccaa tggaacgcac ttcatgatca acattgcttg gtaatgttcc cataacgaat
    52921 ccgttatctt cattatagaa aacatcagct aagcctaact cttctaattc ttttttcaat
    52981 tcttgcgcaa aagcaacttg tgtctgagtc gatggagtcg ttttgcttgt tgcatcggag
    53041 cgtgtttctg ttttgacata tcgtaaaaaa cgtggcaaaa gattttcgta catagtattc
    53101 tctcctttta attaaattga aatgggtttg tatttgtttc agaaacgaaa aagttaatat
    53161 cccaattttc ttcttgtttc caagattcaa atttctcgat aaatcgttgc ttgcataacg
    53221 attcaatata atgaccggga tcaattgcaa tcaacccagc gctttgcata tcatgagccg
    53281 tatgataata gatatctccc gtgatataga catctgctct ttgggcgatt gcttgcggat
    53341 aaaatttttc accgcttcca ccgcaaatcg caatgcgctt cacagaggat tttgcatttt
    53401 ttggctggac tattctcagt ccatctagtt ggaacgcttc ttttacttgt tctacaaatg
    53461 cctctataga aatttcttgc ggcaattcac ctactcgtcc taacccaaac atttcgactg
    53521 gttcatctat agcgaaaaga tcataagcag gttcctcata aggatgagct gaccgcatgg
    53581 cttgaatcac ttgtttttcg atagtttccg gcaaaataac ctcaactttt gcctcttgaa
    53641 cttgttctgt cttccctact ttcccaatcg ctggttgtgc accagcttcc ggagtaaatc
    53701 gaccgtgtcc aatcgaggta aaacttgtac cggtataatc accttgtgta ccagcaccag
    53761 ctgcagctaa tacttcacgc attttctgtg catgatccac aggtacataa acggccagtt
    53821 ttttataatg aatctcatga gtctttacta aataactctc cacttcaata ccgagaagtt
    53881 cacaaaacca gtcattcagt ccatcccaaa taatatccat gttcgtgtgt gctgcataga
    53941 cggcaatatc atgtttcaac aaatctgcat acattttttc ttgtggctga tcagtaacca
    54001 gccgttttac aggacgaaag attggaggat gtttagcaat cagcaaatcg atttttttct
    54061 tgattgcttc ttcgaccact tctggacgaa cgtctaatgt catcattact cgttggattg
    54121 gcttatccaa tgttccgata tgcagtccga ctgggtctcc ttcttcagct agccactgag
    54181 gacaatagct ttcaaacttt tggatgaaag tgcgtccgct gattgtcatg gctttaaaac
    54241 ctcctcaatc atttggattt cctgctcgat ttgtttttga cgtgcctctt gattagctgc
    54301 ttgtttgagc tgttcacgga tagcttttct ttgttgaagc tctcttgtcc attttgattt
    54361 gaagatctca cttttctctt caagcagaaa tggcccgaac atcagctcca tttcattgta
    54421 agtaactggt ttttgttctt tttcggctac aatgatttca tatatttttt tattttcttc
    54481 aagtattttt tctgattgga ttttatactg attttcttgc agccaagatc gtaatgtacg
    54541 ctctccgatg tttggctgaa ggatcaaacg ttcttgtccg cttaagcgtt gattcaactt
    54601 gccagcttct aggatatctc ggatcagtga acctcccata cccgcaatcg tgacagctgt
    54661 aataccatct tgtggctgga tagcatctag accatttgca aggcgtacgg tgatacggtc
    54721 ttctaatcca tcttttttga cttgcttacg tgctgattca taaggacctt gaacgacttc
    54781 accagcaact gcatattcga tttttcctct taatatcaat gcaacgggta agtaagcgtg
    54841 atctgatcca atgtctgcta aacgagcgga ttcagggaca aagtcaccca ctgcggctaa
    54901 gcgctgagat aaatctaaat ggttcatagc ttcactcctt ctagataatt atagcatttt
    54961 ccaaataaaa taaggagact aggaataaaa aaattcccat atctccttaa aaaatatacc
    55021 ttttcgttct ataaaacaac attttcttat ttttgaaaaa atgttattgg ttatttgttc
    55081 aatgaaacat caataatcga atctccagga acttgcggtg ggatagacat cgtcatgatc
    55141 ggcatctcaa ctaatgtaaa ttggactttg tcaccgattt tcaattcatt tatgtccacg
    55201 cttatttttt cagcagtagc atttaagatc actccgtcat tgctgaatga cgttcccata
    55261 ttcgctggat cttctttggc catgacatcg acgagtttca actgaagcgt ttcttcttct
    55321 gcttctactt gagtgatact ttcaatcgtt ccgacaaata tgctttgtct ctccgttttc
    55381 gtactgcttg tttttactgt ttgacccgca tcaatcgaat caacagaacc tgtgttgcta
    55441 tctgcgctac cgcagcctgc taaaagaaaa aatagaccaa ctacagatat actagaaaaa
    55501 atagccctca taaaaaatcc tccgtttaag tttctgcatt acttgttgcg gtgattgtct
    55561 ccggtcattt ctgtccatat aatgattcgc tgacctgctt ttactactag gtcaaatgtc
    55621 agttggactt gtcaactgtt caaccattgc gtcggttgcg accagtcatt tcgatttctt
    55681 ttgtcaaata acgtatgcgt tccattatcc gtttcgcttt tagcggctct tcatctccac
    55741 gttgagtgac ggacaaatgc tgttccaatt catccattgt aaaattcgat gagaagaagg
    55801 ttggtaactg ttcctgcatc cgatattgaa ggataacacc gaatacttcg tctcgtatcc
    55861 aactactcat cgcatctgca ccaatgtcat cgatcatcaa aataggtgaa cgtttcactg
    55921 catctaactt ttctgccact tgatcttttc cgattgcctg tttcatctca acggcaaatg
    55981 tcggaaaatg gactagcgtc gtggtgaatc cagcaatcgc taattctctc gctgttgctc
    56041 ctaataaata tgtttttcca ataccaaaac ttcctactag atacagtcct ttatgatacc
    56101 cttttggatc agcagtatat ttctctacaa aatctagggc ttctaagctc gcatatcctc
    56161 gtccatctgt acgttcaaag ttttcaaaag aagccgattg aatatctttt ggcatatcca
    56221 ttgcttttac tcggcttctg atttcttctt gtctttgccg tgctaacaat gcttccgttg
    56281 gtacataagt gacatccaca taatgaaaat tcaacgtcag cttcggctca tagccgggag
    56341 cgatcatcgt tggatcgttt ttttgatatt tacgcttttc ttgcacgaat tcatacaatt
    56401 tagcgtaact tttttcaata tccgcttgtg tcaattcttc ttggtgtgcg cttagaaaag
    56461 cttgaacatc cgcatcattt aagacttcag cgatcatttc agcatacttt tcttgataat
    56521 tgcgtttgct gatgatcctc ttcaattctt tgcctacgtc ttccactaga tatcaccctc
    56581 cttttttgcc aggtattctt taaaacggcg gtcaatctct gcttttttct cgctgctgat
    56641 ctgtttttca tcttttggct ggttgaccca atcaggaagc ttttcccgtc gaacatttcg
    56701 cttgccagtt tgataatttg tttgcttctt ttgttttgat tgttttgctt gattacttat
    56761 ttgccgaatg tgctggatcg cttcttctgc tgtggcgatt ttcttttgtg cccattcatt
    56821 tgccactgta tttacataac tggcatttaa gctagcatta ttcttgatca ctagtacata
    56881 attcagcaaa acattgatca cgctactgga aagacctgat tgagaaacaa gatcttgcag
    56941 taaccagttt tcttgtttag aaacatatcc gcctttttct gacttgattg cttccagata
    57001 tttcatcggt ggaatgctct gtgcttcttt tatcagctga agcgctgcgt ttgtcaaatg
    57061 ttcgtttgac gagggttcat tactgttttc agaaaccgca actgtttctt ttgtttgctt
    57121 caccggacgt ctctgcattg ccaaaatggc tttttgcagt tctttttcag atactttttg
    57181 ttcactaaag tcataagatt gactgatcag ttcgccaagt gtcaattcat cgaaaccgta
    57241 aagctgatga tacatgtcga cttgtttgat gattggatca tcaagacttg caatatgttt
    57301 ttttgccagc caatcattca aaaactgcca atcgaatgtc gaatctgctt caagtaatgc
    57361 tactgattca gattcttcaa aagatttatt cagcgattcg atgttttctt gattagcagc
    57421 gtacactgct tcattgaagc gataaacatc taaaaatttt tttgtgattt tttgatatcc
    57481 ctctgctgta tgccttttag gttcaaagaa ttgggtaagc ttctggaatc gtcgttcacc
    57541 aactttttcc agaagaagaa aagccagaac agagtctttg aaaaatgctt taggaatgag
    57601 tggttctaac agctcataaa taaaacgagg tcccagatca ctatcttctt taaaataaac
    57661 atccagtaag ccgattcctt ctagcttttt tcttgcatca taaaattgcg ggataccgca
    57721 gcctagacta ctaagcagat ctgcatgcat cccttcagga cctttgccag attcttgaga
    57781 gatttctgat aacagggtga aatagatact caaggcttct ccgccgatta gtggctgata
    57841 aagatacagc aaggcgttct tgctgtcttc tctcagcgga gccgcttttc ttccttggta
    57901 aatatttttt ggttgaagct ctttccaagc attttccaag gaaatgcctc ctttttgttt
    57961 cttaattgtt ttctttatta ttctctttat tattttcttt tgctttatcc acgatatctt
    58021 gcaactcttt taagaaaaca gacatatctt tgaactggcg ataaacactt gcaaaacgaa
    58081 tataagcgat ttcgtctaaa tcaactaaat cttccatcac atattcacca attagattcg
    58141 tcgatacttc gttttctccg agactgcgta cgcggttttc gacatggttg acgatttgtt
    58201 ccatttgttc catagctaca ggtctttttt ctgctgaacg gatgagtcct cttaaaattt
    58261 tatcgcgatt gaactcttcg cgttccccgt ttttcttgat tactaataac ggagcagctt
    58321 caattcgttc aaatgtagtg aatcggaacg aacaattctc acattcgcga cggcgacgga
    58381 tcgcacgtcc atcatctgct tgacggctgt cgattactcg tgaattatta tgttgacatc
    58441 tgggacatct cattttgtct cacctcttgc atcgcatttt atataaagga tgtgtctttc
    58501 tttacaacta gttgtattat aacacgaaac aaatcacaaa cgcgactgtc ctacccaaaa
    58561 aacacggata taagaacaat tttttctact ttcatgcgta attgtgtccc ctgtccttac
    58621 taaatttcaa ttttccaata ctgatcacct tgattactga gaaataactt acacaaaagc
    58681 tgatttagtc atctagcaca aaaaacttga agcagatgaa caccttctca tctgcttcaa
    58741 gtcacatctc gattttttac tattttataa cttctcttcg attcctgtat agtgtttcat
    58801 tcgctgttcc tcgatttttt gctcatcggg aatgtggtca ctttctggga gcggtgtttt
    58861 actacgatca ggtgtcggat tttttcgttc ttgagtcaaa tcttgccgct tcgcttcttc
    58921 ttctgcctgc tggtcttcta tttcatcgat tcttgcttgg tactgctttt ctttttcttc
    58981 tgctgtttct gattgcgtac gaccttcatc gattagattt tctttattga gcatctcgcc
    59041 gattttttct tttgcgttac ctacgacttt atccttttga ttttttaatg atcccatcgc
    59101 tatccctcac tttctatata ctcaggatag caaaattgac acagatactc aaagaaagcg
    59161 attagcaaaa agccaa
//
LOCUS       DANKHJ010000011        59114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000011 DANKHJ010000000
VERSION     DANKHJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..516
                     /locus_tag="PW113_000778"
     CDS             <1..516
                     /locus_tag="PW113_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2525109.1"
                     /translation="LQRFDGIRYGYRSENVKSLDDVYVNSRSEGFGEEVKRRIMLGTF
                     SLSAGYYDAYFKKAGQVRTLIKRDFEKVFADYDLIIGPSSPTVAFGIGENINDPITMY
                     MSDILTIPVNLAGLPGMSVPAGLSEGLPVGLQIIGNYFDEKTMYQAAYAFEQATEFHK
                     QQPAILGGKDE"
     gene            513..1943
                     /gene="gatB"
                     /locus_tag="PW113_000779"
     CDS             513..1943
                     /gene="gatB"
                     /locus_tag="PW113_000779"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2525110.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            1953..3008
                     /locus_tag="PW113_000780"
     CDS             1953..3008
                     /locus_tag="PW113_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2525111.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            3119..4495
                     /gene="rlmD"
                     /locus_tag="PW113_000781"
     CDS             3119..4495
                     /gene="rlmD"
                     /locus_tag="PW113_000781"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2525112.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            4704..5540
                     /locus_tag="PW113_000782"
     CDS             4704..5540
                     /locus_tag="PW113_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2525113.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            5689..5910
                     /locus_tag="PW113_000783"
     CDS             5689..5910
                     /locus_tag="PW113_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525114.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            5911..8628
                     /locus_tag="PW113_000784"
     CDS             5911..8628
                     /locus_tag="PW113_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDL2525115.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            8621..10576
                     /locus_tag="PW113_000785"
     CDS             8621..10576
                     /locus_tag="PW113_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2525116.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            10566..11702
                     /locus_tag="PW113_000786"
     CDS             10566..11702
                     /locus_tag="PW113_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2525117.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(11753..11956)
                     /locus_tag="PW113_000787"
     CDS             complement(11753..11956)
                     /locus_tag="PW113_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525118.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(11972..13804)
                     /locus_tag="PW113_000788"
     CDS             complement(11972..13804)
                     /locus_tag="PW113_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2525119.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(13807..14253)
                     /locus_tag="PW113_000789"
     CDS             complement(13807..14253)
                     /locus_tag="PW113_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525120.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(14246..15196)
                     /locus_tag="PW113_000790"
     CDS             complement(14246..15196)
                     /locus_tag="PW113_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525121.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            15591..16208
                     /locus_tag="PW113_000791"
     CDS             15591..16208
                     /locus_tag="PW113_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525122.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            16305..16511
                     /locus_tag="PW113_000792"
     CDS             16305..16511
                     /locus_tag="PW113_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525123.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            16516..16830
                     /locus_tag="PW113_000793"
     CDS             16516..16830
                     /locus_tag="PW113_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HDL2525124.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            16831..17973
                     /locus_tag="PW113_000794"
     CDS             16831..17973
                     /locus_tag="PW113_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDL2525125.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            17975..18517
                     /locus_tag="PW113_000795"
     CDS             17975..18517
                     /locus_tag="PW113_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDL2525126.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            18578..20515
                     /locus_tag="PW113_000796"
     CDS             18578..20515
                     /locus_tag="PW113_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDL2525127.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            20726..21496
                     /locus_tag="PW113_000797"
     CDS             20726..21496
                     /locus_tag="PW113_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL2525128.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            21489..21833
                     /locus_tag="PW113_000798"
     CDS             21489..21833
                     /locus_tag="PW113_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HDL2525129.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            21826..24069
                     /locus_tag="PW113_000799"
     CDS             21826..24069
                     /locus_tag="PW113_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2525130.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            24207..24416
                     /locus_tag="PW113_000800"
     CDS             24207..24416
                     /locus_tag="PW113_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525131.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            24413..24697
                     /locus_tag="PW113_000801"
     CDS             24413..24697
                     /locus_tag="PW113_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDL2525132.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            24697..26049
                     /locus_tag="PW113_000802"
     CDS             24697..26049
                     /locus_tag="PW113_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2525133.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            26046..26474
                     /locus_tag="PW113_000803"
     CDS             26046..26474
                     /locus_tag="PW113_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL2525134.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            26648..27007
                     /locus_tag="PW113_000804"
     CDS             26648..27007
                     /locus_tag="PW113_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2525135.1"
                     /translation="MKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAINKR
                     YGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCKSC
                     HSKIHMTRRNTKDKTIM"
     gene            27141..27713
                     /locus_tag="PW113_000805"
     CDS             27141..27713
                     /locus_tag="PW113_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2525136.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            27728..28510
                     /locus_tag="PW113_000806"
     CDS             27728..28510
                     /locus_tag="PW113_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HDL2525137.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            28510..29745
                     /locus_tag="PW113_000807"
     CDS             28510..29745
                     /locus_tag="PW113_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL2525138.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            29819..30196
                     /locus_tag="PW113_000808"
     CDS             29819..30196
                     /locus_tag="PW113_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HDL2525139.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            30198..30392
                     /locus_tag="PW113_000809"
     CDS             30198..30392
                     /locus_tag="PW113_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525140.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            30376..30552
                     /locus_tag="PW113_000810"
     CDS             30376..30552
                     /locus_tag="PW113_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HDL2525141.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            30549..30692
                     /locus_tag="PW113_000811"
     CDS             30549..30692
                     /locus_tag="PW113_000811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525142.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            30775..32340
                     /locus_tag="PW113_000812"
     CDS             30775..32340
                     /locus_tag="PW113_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2525143.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            32396..33130
                     /locus_tag="PW113_000813"
     CDS             32396..33130
                     /locus_tag="PW113_000813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525144.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            33196..34524
                     /locus_tag="PW113_000814"
     CDS             33196..34524
                     /locus_tag="PW113_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2525145.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            34424..35179
                     /locus_tag="PW113_000815"
     CDS             34424..35179
                     /locus_tag="PW113_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2525146.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            35183..36394
                     /locus_tag="PW113_000816"
     CDS             35183..36394
                     /locus_tag="PW113_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2525147.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            36405..36716
                     /locus_tag="PW113_000817"
     CDS             36405..36716
                     /locus_tag="PW113_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2525148.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            36713..37051
                     /locus_tag="PW113_000818"
     CDS             36713..37051
                     /locus_tag="PW113_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2525149.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            37044..37475
                     /locus_tag="PW113_000819"
     CDS             37044..37475
                     /locus_tag="PW113_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2525150.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            37472..37813
                     /locus_tag="PW113_000820"
     CDS             37472..37813
                     /locus_tag="PW113_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525151.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            37816..38406
                     /locus_tag="PW113_000821"
     CDS             37816..38406
                     /locus_tag="PW113_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2525152.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            38416..38796
                     /locus_tag="PW113_000822"
     CDS             38416..38796
                     /locus_tag="PW113_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525153.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            38844..38984
                     /locus_tag="PW113_000823"
     CDS             38844..38984
                     /locus_tag="PW113_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525154.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            39003..41564
                     /locus_tag="PW113_000824"
     CDS             39003..41564
                     /locus_tag="PW113_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2525155.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            41635..42036
                     /locus_tag="PW113_000825"
     CDS             41635..42036
                     /locus_tag="PW113_000825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525156.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            42113..42805
                     /locus_tag="PW113_000826"
     CDS             42113..42805
                     /locus_tag="PW113_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2525157.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            42802..46692
                     /locus_tag="PW113_000827"
     CDS             42802..46692
                     /locus_tag="PW113_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2525158.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            46702..46869
                     /locus_tag="PW113_000828"
     CDS             46702..46869
                     /locus_tag="PW113_000828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525159.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            46856..47263
                     /locus_tag="PW113_000829"
     CDS             46856..47263
                     /locus_tag="PW113_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HDL2525160.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            47264..47683
                     /locus_tag="PW113_000830"
     CDS             47264..47683
                     /locus_tag="PW113_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2525161.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            47673..48581
                     /locus_tag="PW113_000831"
     CDS             47673..48581
                     /locus_tag="PW113_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2525162.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            48697..49734
                     /locus_tag="PW113_000832"
     CDS             48697..49734
                     /locus_tag="PW113_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDL2525163.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(49795..50085)
                     /locus_tag="PW113_000833"
     CDS             complement(49795..50085)
                     /locus_tag="PW113_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525164.1"
                     /translation="MEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIEAEEAL
                     YQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            50395..50568
                     /locus_tag="PW113_000834"
     CDS             50395..50568
                     /locus_tag="PW113_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525165.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            50629..52182
                     /locus_tag="PW113_000835"
     CDS             50629..52182
                     /locus_tag="PW113_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2525166.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            52146..52595
                     /locus_tag="PW113_000836"
     CDS             52146..52595
                     /locus_tag="PW113_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525167.1"
                     /translation="MRTTFEREVTRMNNTIPYGYVIKNGEINVDEQQAQQIREFFNIY
                     LEGVGLQEAGKRSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIK
                     FFKKRKEIKVPDTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            52597..54147
                     /locus_tag="PW113_000837"
     CDS             52597..54147
                     /locus_tag="PW113_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2525168.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            54270..58793
                     /locus_tag="PW113_000838"
     CDS             54270..58793
                     /locus_tag="PW113_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDL2525169.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            58928..59056
                     /locus_tag="PW113_000839"
     CDS             58928..59056
                     /locus_tag="PW113_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2525170.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT        19015 a        10458 c        13306 g        16335 t
ORIGIN      
        1 ttgcaacgat tcgatggtat ccgctacggt tatcgctcag aaaatgtgaa atcattagat
       61 gatgtatatg tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg ccgtatcatg
      121 ttaggtacat tctccttaag tgctggttat tatgacgctt acttcaaaaa agcgggacaa
      181 gtccgtacat tgatcaaacg agattttgaa aaagtctttg cagattatga cttgatcatc
      241 gggccatctt caccaacagt tgcattcgga atcggtgaga acattaacga tccaatcaca
      301 atgtacatga gtgacatctt aacgatccct gtcaacttgg ctggacttcc aggtatgtct
      361 gtaccggcag gtttatcaga aggattacca gttggattgc agatcatcgg taattatttt
      421 gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac aagcaacaga attccataaa
      481 caacaaccag ctattttagg agggaaagac gaatgaattt tgaaacagtc attggactag
      541 aagtccacgt cgagttaaaa accaactcaa aaattttctc tccagcgcca gctcattttg
      601 gtgcagaacc aaacagcaat acaaacgtga tcgactgggg ttatccaggc gtgctgccag
      661 taatgaataa agctgcgctg gaattcggga tgaaagcagc tttggcatta aactgtgaaa
      721 tctctaaaga tactcatttt gaccggaaaa attactttta cccagataat ccaaaagcat
      781 atcaaatttc ccaatttgat caaccaatcg gtcatgacgg atggatcgaa atcgaagtag
      841 aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt agaagaagat gctggaaaaa
      901 acatgcatgg tattggcggt tattcttacg ttgatttgaa ccgacaaggc acgcctttga
      961 tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga agcatacgct tatctagaag
     1021 cattgcgatc aattatccaa tttacagaag tcagcgatgt gaaaatggaa gaaggctcta
     1081 tgcgctgtga tgccaatatt tctcttcgcc cttatggaca agaagagttc ggtacgaaag
     1141 cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa aggtttagct tttgaagaaa
     1201 aacgtcaagc aaaagttctt ctttcaggag gagaaatcca acaagaaaca cgccgttttg
     1261 acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga agggtctagt gattatcgtt
     1321 acttcccaga gcctgatatc ccgaaattcg tgatcgatga cgaatggatt caaaaagtaa
     1381 aagctagttt gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt gagtttggac
     1441 ttccagagta tgacgcaatg gtattgacac ttacaaaaga aatgtctgat ttttttgaag
     1501 ctgttttgga aaatggtgca gatgccaagc aagcatctaa ctggttgatg ggtgaagttt
     1561 ctgcttactt gaacagtgag aaactggaat tagctgagac aaaattgaca cctgagaact
     1621 tagcaggaat gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc gccaaaaaag
     1681 tcttccgtga attgatcaca aatggcggag aagcccaaga agtagttgaa aaaaatggtt
     1741 gggtgcaatt atctgatccg aacaaactac tgccaatcat taatgaaatt ttggataaca
     1801 accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc ttcctagtcg
     1861 gtcaaatcat gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat aagctactta
     1921 aagatgaatt agcgaaacga tagaggctga aaatgaaaaa agcacgcgta atttataacc
     1981 ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga tatcctacag gcattagaag
     2041 aagcaggatt tgaagccagc gcctatgcaa ctacaccaga accagattca gccaagaatg
     2101 aagcatgtag agtcgcagaa ctaggttttg atctagtagt ggcagctggt ggcgatggca
     2161 caatcaacga agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa atggccatca
     2221 tcccagctgg tacaacgaac gactatgctc gagcattaaa aattcctaga gataatatta
     2281 aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa aatggacatc ggcctttctg
     2341 ataacagtta cttcatcaat atcgctgcag gtggctattt gacagaattg acttatgaag
     2401 ttccatcaga actaaagagt atttttggtt atcttgctta tcttgcgaaa ggagcagaga
     2461 tgctccctcg agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg gaatatatcg
     2521 gaaatgcttc gatgtttttc ctagggctga cgaactctgt tggaggattc gaacagatcg
     2581 cgccagatgc caaattggat gatgggaagt tttctttaat cattgtgaag acagcaaata
     2641 tctttgagat cttgcatatt gcagcactca tgctgaatgg cggtcgacat gtcgatgatc
     2701 caagaataat ctataccaaa acaagcagtt tgttcgttga aacagaagat gatatcaatc
     2761 gaccagtaat gatcaactta gatggtgaat acggcgggga agcaccgatg catttccaaa
     2821 atcttcatca acacattgag ttctttgcca ataccgatca gattcctgat gaagcaatca
     2881 caggtacaga tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa gaagtcgaac
     2941 gtttaacgga tgaagatatc gacggtgacg gaaaaatagc agacgaaaca cctaatcaaa
     3001 agaattaatc aaaagccggt gctgaacgta gtaaggtcag tgccggtttt agttgtgtaa
     3061 ttagctgatt tatagtagag ttcagtagga ttgaaaaata ggcttaaagg agcaaaatat
     3121 gacagaacag caaatagtaa agaaaaacga acgatataca gtcgaaatcg tcgacttgag
     3181 ttatgaagga atgggggtag caaaaatcga tggttatcca ttatttatcg aaaatgcact
     3241 ccctggagaa gaagtagaaa tactagtagt aaaagcaggc aataagtttg gatatggaaa
     3301 agtcgaaaca tttattacac aatcacctga tcgtcagcct gtagaaaaca atgcattact
     3361 tcgcataggt atcgcacctt tagcccattt gaagtatgaa caacagcttg tcttcaaaca
     3421 aaaacaagta gagcatgtaa tggcgaagat tgctaagatg ccagaagttc ctgtacttcc
     3481 aacggtcgga atggcagaac cattcggcta ccgtaataaa gcacagatcc ctgttcgccg
     3541 tatcgatgga cagttagcca ccggttttta caaaaagaat agccatgatc tagtccctat
     3601 cgaagatttt tatatccaag atcctgtcat cgatcaagca atcaaagtag tcagagatat
     3661 cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa aatgaaggac agcttcgtca
     3721 catcattatc cgtcgtggtc attattccca tgaaatgatg gtcgttttag ttactcgtaa
     3781 agaaaaattc tttaaatcca aggaaattgc tgaagctatt catgaagaat tgccagaagt
     3841 ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat gtgattttag gagacaaaga
     3901 aaaagtttta tatggcagag actatatcga agatcagcta ttaggaaaaa cctatcgaat
     3961 ctcagcaaag tcattttatc aagtaaatac tcaacaagca gagtatctgt accaaacagc
     4021 catcgagttt gccgagttag ataaaaaaga cattgtgatc gacgcttatt caggaatagg
     4081 tacgattggc ttgtctttag cagacagagt ggataaggtg tatgggatgg aagttattcc
     4141 agaagcaatt gaagatgcac agttcaatgc tttaaataat aaaatcgaaa atgttcatta
     4201 tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga tcaaaccatc
     4261 tgtgatcgta gttgaccctc cacgaaaagg attagatcat cattttattg aatcagcaat
     4321 cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt ttgcaagaga
     4381 tgctaagtta tttgcagaag caggttatgc cgtgaagaaa gttcagcctg tagatctttt
     4441 cccgcaaacc catcacatcg agtgtgtagg actgcttaca cgtgtggaac gttgatatga
     4501 caaggattag agcgattcgg gatggtttcc ggattgctct tttttcgtgt tgaaatgacg
     4561 atttttgggt gagcgtagag ttgatggaaa tgctatggag ctttgagttg ataggagtta
     4621 caccgtagac ttgataggtt agatgatttt gataacggtt acgtgatata atatattatc
     4681 gagttttcaa gggagacaaa cttatgaagc aaatttttat attttcagat acacagaaag
     4741 ataatatttc ttattttgaa cgcgagccag caatcttggt ttcaatgagt cgtagtgatt
     4801 tgccgtattt gaaggaacag gatttttcaa attatccagc tgtttatgtt ttgattggtg
     4861 gtaataagcg ttacgtgggt caggcagcag ggcaatcaat ttcacttcgt ttatctcaac
     4921 actttctcaa ggaatataaa gcttgggttg aatcagtttt attctttaca agagcagatg
     4981 ggaaaatgtc aaaagcagat acagattatc ttgaaaaacg acttatccaa gatttccaag
     5041 aaaaatctga ctatgaaatg atgaattcaa ctgctggtaa tacttcgtat attgataaac
     5101 ttcaaaaagc aaaatcggat cagctatatg acacagtgtt tgaaattata gatgaaattg
     5161 caaatatcga tttgtttggt acttccgaag acagtgaaac ccaaatttat gaatttagtt
     5221 catctggaca atatgaaatt gagtatgatg ggaaaaagat atcttcgaaa tctgctagag
     5281 gtttattggt tgattttgtt tctcagctat ttgcggatgg aaactacaac aatcaactgc
     5341 aagaattaat tgtcgatgag acgccgtcgt tcgctataat tttaggacgt aaaccatcca
     5401 tgtataacgg aagaccaaat tcagccaggg tagctgatgg agtatgggtt tatgcaaatt
     5461 tcaacaaaaa gaatgtcaga atcaaaattg ataaactagc tcgtcaattg gggattccgg
     5521 ttaaaataaa gtggaattaa tatttgttat ctcgccttta gtaaatctat gctgaaggct
     5581 tttattttag aaaaatagtt tgcgttcgcg aaaatatttc atgtttcgta taataatgat
     5641 agctcataga tgatataatt gaagatacca gtagaataga ggtgtagtat gtcaatttct
     5701 tataataaat tgtggaagtt attgattgat ttgaatatga acaaaactca acttcgtgaa
     5761 atatctggcg tcagtcccaa tgtaatagca aaactcggta aaaatgagtt ggttgcaatg
     5821 gagtcattat tgaaaattgc tgctgcctta aatgttgatg ttagtgaaat aattagttca
     5881 gatgttatag atgcggagga tgttaaataa atggcaaaaa ataaagtttt aaacatcact
     5941 gaaattgaag atggtgtaaa aaaaatactt tcaaataggg gcgatgattt catcactcaa
     6001 tttttgtcct tgtacgatat ccccaaaacg tcgattacac gtgcaaaatc aaaattcgat
     6061 aatggtgaac catttgtgat taagaacaaa cttcattatg aagaaattga aggcaacgtt
     6121 gtcattgcga ttgatgcgat tgctcaatca atcaaagagc aaaaatctaa accacgatat
     6181 atcttggtaa acgattattt agatattgct gcaattgata cgaaaactca tgaaactttg
     6241 aatattccaa ttgaagagct accggctaat gctgactttt tcttagcgtg gaatggcatt
     6301 gagaaggctg attatcaagc tgaaaatcca gctgaccgaa aagctgctga acgttttgca
     6361 aaattgtatg atgtggtggc taaagataat cctgatgcca acgagcatgc atttaacttg
     6421 ttcttgattc gagtgctgtt cttgttgttt gcagaagata caggaattat gaataagggt
     6481 tcctttacaa atgttctaaa aacaagaacg gctgaagatg gttcgaattt taacgaagta
     6541 atcaaagaac tctttgcaat cttggatatt aatgagttag ctcgcaatgg caaggatgag
     6601 tggctacttg aatttccata tgttaatgga aaactgtttg gtgagccaca tgtggatttg
     6661 accttttcaa aagtttcaag agagctgctt attgaagcag gggaattatt gaattggaat
     6721 gaaatcaatc ctgatatcct tggcgcaatg attcaatcag ttgcttcagc agaagaccgt
     6781 cacgttgccg gtatgcatta tacaagcgtg ccgaatatca tgaaagtcat caagccgtta
     6841 ttcttagatg atttgacaga gtcatttgaa actttaaagc ttcgcagtga agaaaacgag
     6901 ctgaaggaca ttactgaaaa aacaagaaac gataataaga gaacaatcat caatgatcta
     6961 agtgctttgt tgacgcgaat ttctaatatt aagtttttgg atcccgccag tggttctggt
     7021 aattttctga tcattgctta taaggaaatc cgtagacttg aaattaagat tttgcaattg
     7081 ttgggcgact tacaaaataa tgattccatg ccactatcag tgattcatct tggtaatttt
     7141 aatgggattg aattggatga ttttgcgcat gaagtagcaa gactttcatt atggattgct
     7201 gaacatcaaa tgaataaaga gcttgaagaa gcaataccag gtaccattgc tgagttgtta
     7261 ccgttaaaag atgctgggaa tatagtgtgt gccaattctc ttcgaattga ttggaatgag
     7321 gtgttgcgac tcaaagaaaa tcatgaaata tatataatgg gaaatccacc gtatctgggc
     7381 aaaagtcttc aaaatgctga acaacgtgaa gatatggaaa aacttgttgg cgatattcga
     7441 aaatataaat tgcttgatta tatttttggg tggttttgga aagctaaaca atatattgca
     7501 acatctcatg gctcgttcgc atttgttact actaattcaa tttgtcaagg tgaacaagtt
     7561 agtatccttt ggccgtacct tttaaaagat gaggtaagaa tttcatttgc atatcagagc
     7621 tttaagtgga acaacaatgc aaagaataat gccggtgtga ctgttgtcat tattggtatg
     7681 acaaataatg tgaatattca tcctaagtta tatttagaga atgggtcagt aatgagtgtt
     7741 tcaaatatta atgcatattt agctaatggt gaaaatgtat ttgtcaacga atcaagaaat
     7801 agtatttcaa atttaccctc tttgaaattt ggcagtatgg caaatgatgg tggtaatcta
     7861 gttttttcag tggcagaata ctggaatttg attcaaaaat atccagatat taaacattta
     7921 tttaagaaat atttaggatc aacaggatta ataaatggag acttaagata tactttatgg
     7981 ttgaccgagg aagatgcaac tagatacaaa caaaattctc ttattaaaga aacaataaaa
     8041 aatgtaaaaa gttataggga aaaaagcaag agaaaagaaa ctcgagcact agctgagaag
     8101 ccatggtcat ttggtgaagt tcgttatgat gaaaccagtg agaatagtat tctaattcct
     8161 cgtgtgtcat cagaaaaaag agattatgtt cctatgggta tagttgggag cgatactata
     8221 atttcagatt cggctatggc aatctataac gctccaatgt ggctgcttgg tctgttagaa
     8281 tcccgaatgc atatggtgtg gctgcgctct gtcggtggga aattagaaac tcggtaccgt
     8341 tattcagccg ggttagtcta taatactttt ccgattcagg atttatccgc ccagcgtaaa
     8401 aatgaaatga ctcgcgtgat gacagaaatt cttgatttac gagaatacga aggcggcacg
     8461 ttagccgatt tgtataataa agacagtatg ccagaatcat tacgtaagaa acaccaggaa
     8521 ctggatggga ttgttgatag agcttatcag caacgcccgt ttgaatctga cgaagatcga
     8581 cttggaacat tactaaaact ctatcaggag atgacaatca atgactagta atttatttga
     8641 aattaatttt gataaaaatc aatcaaaaac aacaaatgac ctcggtatgc gtgaaatgca
     8701 agaacgtgtt tatgaaaagc gtgcgtcaca gtatttgttg gtaaaatcac caccagcttc
     8761 aggaaaatca cgtgcattga tgtttgttgg cttggataaa ctccataatc aaggaattaa
     8821 aaaagtggtt attgcagttc ctgaacgctc aattggtaaa tcatttaaag atactgaatt
     8881 atcgaagttt ggtttttatt gggattggga agtaaagcca agtgacaact taactattgg
     8941 gggcggtgaa aaatcaaagg ttcaacactt tgttaaattc atgaactcga ctgacccaag
     9001 tgataatatc ctgattgcca cacatgctac gttgcgattt gcttttgaag agttggacga
     9061 ttcattattt gacaatacgc tattagcgat tgatgagttc catcatgtga gccgtgatga
     9121 taaatctgtg ttaggtaatg ccttacgtag catcatgtca aactctaatg cgcatatctt
     9181 agcaatgact ggttcatatt tccgtggcga ctctgttcag attctggaac cagccgacga
     9241 agataaattt gataaggtca cttacactta ttatgagcaa ctggaaggat ataaatatct
     9301 gaaatccttt gcaatggggt atagtttcta tcgtggaaaa tatacagatg ctttggataa
     9361 agtaattgat gtcaacaaaa aatcaattat ccatattcct aatgttaatt ctggtgagtc
     9421 tactaaagat aaatacgcgg aagttgatga aatacttgat ttattgacgg acggtggtaa
     9481 tatcacacag aacgaggatg gtatttatga aatcgaacgt ccagatggta aaaaactatt
     9541 agtggccgac cttgtgaatg aagaaggacg tgaaaaagtc tcagcatatc tagcaagtat
     9601 tacggatgac attaaagatc ttgataagct tgatatcatc attgcacttg gaatggcaaa
     9661 agaaggcttc gattggccat ttgcggaata tgcactgaca atcggttatc gtaactcatt
     9721 aactgaagtg attcaaatca ttggtcgcgt aactcgtgac agccccaata agactcatgc
     9781 acagtttaca aatcttattg cgcagccaga tgcacaggat gacgaagtat tattcgcggt
     9841 taacagtatt atgaaggcaa tttctgcatc tctacttatg gaacaagtgt tggcgccagt
     9901 ttacaagttt aaaccaaaag atggtaagcc agacaaacca ggtgatcttt caatcagagg
     9961 actgaaacct gctgatacga cggaacgtac aaagaaaatc atcgaaaatg atatggaaga
    10021 tttgaaatct agcattttac agagccaaga tattcaaaat gcgattgttt caggtgctga
    10081 tgcgaagatg attaataaga cgttgattcc acgtgtcatc attgatcgtt atcctgattt
    10141 gacgaacgaa gagttagaaa ccgttcgtca acatacagtt gccgatataa atattgcggc
    10201 tggacggaca acaacttcag aagatggtag ccgcgaaatt attaagatgg ccgatcgttt
    10261 cattaacatt gatgaattga atattgattt aatcgatcaa gtgaatccgt tccaacgtgc
    10321 gtatgaagtg attagtcgtg atattgatgc accgactctt cggtttattc aggattattt
    10381 gtcatcaaat aagtatgatt ttactgacga acaattggtt gcggcctacc aagctgctaa
    10441 gaagttccgt gcagaaaatg gtcgaaatcc tgatcgaaat agtaaagatg atggtgaaaa
    10501 atacttggcc tttgctttgc tacgacttgc tgagataaaa cgtgaaagag aggcagcgaa
    10561 agaagatggc gaatagttta gatagcatct ttgaagatga cgcttttgat gatttaacca
    10621 agcaagttga aaaaaagaaa gttgtaaaac aagacccaga agttgctaaa tttcaggaga
    10681 ttatagactt tgtgaatgaa aacggacgag agccagaaaa aactcctgaa tggtctagtg
    10741 aacgcgcatt gtgggctcgt cttagcggat ttcgaggaaa acccgaaagg tctgagaagg
    10801 ttaagaagta tgacactctt ggtttattgg agaaagaatt cgaaccggat ggagagtttc
    10861 tcgctgattc agttgaaatc gatgacgagt tgaatttaga tgatatctta ggctcagatg
    10921 atatgattga tgatttttca gacttgttgg atgtttcccg ctatcgtaga actgtgaacg
    10981 ctgcagataa aattggccgc agaaaacatg caagtcattt tgaaaagtat aagagcttgt
    11041 tcgatcaagt gcatgcagat atcgcatctg gcagacgaaa aattatcccg tttgagcagt
    11101 atgatattca tgaaaataga ttctacatcc aaaacggcgt aatgatgtat atcgtttcca
    11161 tttcgaagga tacctttatc gctgataatg ggaaaacaaa tgccagaatg catgttgttt
    11221 atgagaatgg gacagagaat aagaatctgt tgttacaatc tttagcttcg tctctatact
    11281 cgactgaacg tcatggaagg atggtgacag agccggtaga tgaggaaaca ctggctgaaa
    11341 gctttggaga agagtttacg acaggttata tctacgtgct gaagtcatta agcacgaatc
    11401 cggatattag caagtggaat aacctgtata agattggttt cactcgaaat gatgttgaaa
    11461 gtagaattgc caatgcggaa aacgacgtga cctacttaaa tgcaccagtg aaggtcatgc
    11521 tttctgctga agtacaaaat gtcaacgctc agctacttga gcgcactctc catcatgtat
    11581 tccaggacaa acaagttgtc tttcaggatg gaaacttcaa aaaagctact gaatggtata
    11641 tggttccatt gaatgatatt gaatcgaaaa tcaatgaagt aatcgctgga ttacaaaaat
    11701 aaatatgaaa atgcactgta ctcatgaatc gaatacagtg catttttgat atttaatcgc
    11761 gaacgatttc aacaatatcc gataaatcac aatcaagtgc ttcacagatg cgaagtaaga
    11821 tatctgttgt tacattttcg cctttattta gttttgagag tgttgctgaa ctgatatcag
    11881 tcatttttct gagttgtgct ttattcagtc ctctgtcgat taggagtttc caaagcggat
    11941 tgtagctaaa cttcatgtaa tcattcctgt tctagttata aatatcgtat agatatccat
    12001 agcgtccatt gtttttatct tcagtaaaga caatctgatt tacttgttta tcatcatagt
    12061 ttagtgctgg caagttcgtt gtattctcaa cgatgataag ttgtccttcg ttttgatgat
    12121 ccataaagta tttgaaaagt ccttctttca tattgtcatc gccatcttgt tttttgcgtt
    12181 catctaatcc aagaagaggg gaatcgataa tgaacacaga aggtgtgtac ttagcgtgat
    12241 tatacaagag atgtcgatat gccattacca ctactgaatt gataaacgct gtatatccat
    12301 gtccgttttc ggtccatttt agatttccat tgattttgat gtcgaaaata tccttggcga
    12361 aaaatgcgtt acgaaattca ttaggtttat aatgcatatt acgtaacatg gagtccagta
    12421 actcagacat ttctttgtag aattctgcat caaatttctc tttaggttta taaaggatac
    12481 gttcgtcttc tggctcattc tcgatttttt caagatcact tttaagctta ttttgcattt
    12541 tttccatcga gattagctgt tgtttaatat ccctgtaagt ttcgatttta ttgattcgat
    12601 cctttaacgt ctcagctttc ggttttagtt catcgttgat tgctgcatca agcgatttct
    12661 ttcttgtttt aagctcatca agctgttcca ttaactcttg cagttcaact tcggtatcat
    12721 gagattcttc cgtcaaatct gataattgag agagcaatga atgtaattct gacctagcag
    12781 tatcgatgta ggttgtttgg acaggtttac ttagttcgcc gttacagaat ggacagctat
    12841 gtgaagtatc atgttcatta tgatcattta ataatcgctc accatctacg attagtgtta
    12901 gtcgttcaat atccgattga tattgataat gtaactgttt gtacctattc aaaagaagtt
    12961 gagtttcatg aagatactgt tccttttgat aaatctcttt tatcaagatt ttgctgctgg
    13021 ctaacgcatc attgatttgc ttttcaattg tttcaagttc agcgaacatt ccatcgacaa
    13081 tttgttgaaa atcatcagag ggagcctcag gtaattctga tttagccact tcgtagtttt
    13141 catataattc agaaaggctt gaattgatgt agttaatcac cgagttcctt ctaatttttt
    13201 tatgttcttt tgtttcgttt tcttccgagt tcgcaaaatc tgaaccgtat agcagaagta
    13261 acaacgatga taaaaaagct gtgcgttggg ttgtttcccc aggaaaaaga acagaggcgc
    13321 tggtattgac tctatcttgt ggaataagtg tgctaggcgc taatgtccgc caagttaatt
    13381 tttgacgttt gaaatcctca ttcttgataa tctgataatc atcatcgatg cctaaaagtt
    13441 gtaaccaaaa atggctaatt ggatttttcc cagatcgtgc attatatttt tcataatcta
    13501 catattcacc tgaactcgtt attttggata ccaaaatgga gttgctatca aattctctag
    13561 ttaatgaata tttgccatta gtcgattgaa taatcagttc aactttactg tatccggttg
    13621 catcggttga aaagggagca gcgtcttttt tcgcgccata gagaaaatcg attgccgtaa
    13681 caatgctagt ttttccggta tttgaagggc cagaaattat gtttagtcca ttggtgaact
    13741 tgacagttgc atcttcacga tctttgcctt ttatggtaac acgttcaata taataatttt
    13801 gcattatcat tgctctcctt taaattcctt tgcgcgttgg tttatttgtc gcagaagctc
    13861 aacgtcactt ttatcttgaa tgtatttaac agattcgtca gctatttgta agtatttttc
    13921 tgcatatacc gatacgaatt gtgatatcaa ctttaggcca tcttctgata aagcatactt
    13981 aaaaccttct ttgctttgcg atggttggat aacatgttgc agtacaagat ttttgactgc
    14041 atctttaact aattcacgtc gcgcaataaa ttcactgaat ctatagctgt tatcaccatt
    14101 taagttgtaa tcggaaattt caaagtcgcg accatacgtg gtgatgaagt ctgtgattaa
    14161 aattctatca gcgctgacag gctctttaaa ttcagagagc agtaacatta ctcgaaccga
    14221 aatttcaaat ggagtattaa acaaactatt catcttcagt cacccatttg atctttccgt
    14281 cattaaccag catgtggcaa atgccttttt tagcagaatt ttgcagcaat cccggtataa
    14341 acgataagat tgatccgttt aatgttataa cagacgactg ctgtagaacg gctttgagtc
    14401 tctcaacgcc atcatcgtag tcttcttcat agacttcctc gatgccgtcg tacatatctt
    14461 cttttaaagc ttcaaagtgc tcagttcctt cctcttgtgt aaagttatca cggaccccac
    14521 gacgtatgct ttcagcttta tagtaatttc ttcgctgtct cttgaaatcc tttagccgcg
    14581 aatttggcag gtcgtttgta tgctcgtata cttcgtatcc atcggcatct gcatatgctg
    14641 aaaaaagagc tatgacatac gtcatctcct cagctctaat atcaagggga actggcaaat
    14701 cgtcaaagaa ttttttcact tcatcattat aagagaaatc accattagtg ttgattctta
    14761 aacttgcgag ctctaattcg ctaagcttcg gcttggcctt ttttgttttt acttttgttt
    14821 gagaagcaat tgaggtgatt tcatcaagta aaagatgggc cactatttca gaaacgtctg
    14881 tatctttggg gattaccaca cctttatcaa tcaattgttg cttaaggacg gttggagcat
    14941 catctataag acgttcataa ataaaagtcg tgcatttgtc gatgttgaat tttccagcaa
    15001 cccgaactgc atactcttgt gttaatggtc gagtaccatt gtaaaatctt cgtaggttat
    15061 catcagaaaa accgaaaatg gtggcctcgt catcatttga aacactatcc agtaaagcaa
    15121 ttaaaaaatc ctgtggcttc acgtactcgc ttcccataaa cggataaagc gtttgagtaa
    15181 tttcacttat ttccaaaata caacctcctt tgttacgtcc gccgttgacc gccgtttgcc
    15241 gccgatgact gacgaaaaca agggaggcct ttttctataa ttaaaacata gtagtttaac
    15301 tatttaattt tatcatttat ggttcataaa cactacatta tgtttcaaga aagtgaacga
    15361 atttatctta aaagcgaata gtcctactaa taaaatataa caatgcctga gttgcaatag
    15421 ggcaaggtta caaatactga aaagaactgg ttatccagaa aatttttcag atattgtatg
    15481 ccttacttta ttgcgctctt ttttcgtgat gagggacggt gtacgtattg gcacggtccc
    15541 tgtttgtttt ccttgctcaa aagctctttc aggcaagaaa gagggatcta atgagtagat
    15601 ttatgaaaac acaccaaaac gaccgcagca cttacaagta cgtgtttgag cgtactgatg
    15661 ccaatggacg tatccaaaaa gaagtggttg agattcgccc aggcgaagac ggcgtcactg
    15721 agcttaacat tcaggagctg catcgcgctg acgaccgtgc gatttacaac ttttataaaa
    15781 acgcacgtcc tgaacgcaca gaagaagaaa aagaaaagat caaggcttgg aaagagaagt
    15841 tccgtgaaga ctttaataat gagcacggct attatccaat ggacgaatac gtggatggtg
    15901 aagccaacga ttacttcaaa cgtaactaca atttgtcgct caatgaattt gatgatgaat
    15961 gcgatgaggg ggacaacccc atccaatcat tggtttacga tgcttaccaa gataatgaag
    16021 aagatcctcg tatcgatcgt ttgcgtgaag tcgtgacgca attattaccg taccaccaag
    16081 aaatctatca catgctcgtc actgaaaaca tgtctcagac tgaagtggcg gacaagcttg
    16141 acaagaaaaa acagtcaatc aatcgcacct ttggaatcat caaagacaag atcaaagaat
    16201 tattttagaa aaaagttacg ggggagttta ctttccccgt tttccttcgc ctgtattgtg
    16261 taagggagta gatcctaaac aaataaagat gaaggaggac catcatgaac ctaaaacaca
    16321 aaatccgagt aaacgtgact gatgacacgg gtaacaaaga aactgtccta caaggtggtg
    16381 tgcgtcattt gcctcgctgg tttgcacggt ggctgttcgg taagggcaac atggacaaaa
    16441 ttctcgtgct cacaccaggg gacacaatcg aatcagtcga aatccgtgaa gttaacaaga
    16501 agggagcgta atgacgtgag taaaacaaaa ctatttcttc aggtcgttga ggacgtccaa
    16561 gcgttagctg atagcttaca agagttggct gtggcgatga aaaccaatga acctgaacca
    16621 caagttgaaa caccagaacc gacaatggaa acaaaatcag aagtcaaaca accgacgctt
    16681 gaagaagtcc gtggtttgtt ggcacgtaaa tctcaagaag gtaagtcaac tgaagtgaaa
    16741 gcactgattg agaaatatgg tgccagtcgc ttgagtgatg tgccgacaga aaactacgcg
    16801 gctttggttg cagatgcgga gggattgtag atgccaacac aacacgcagt cctatccgca
    16861 tcttcaagta atcggtggat tcactgtccg ccaagtgttc gcttgtcaga gcactttgaa
    16921 aacaagtcga gcccgtatgc ccaacaaggc actgatgctc ataccttatg tgagtacaaa
    16981 ctgaataagt tcattggtaa tgacgtcaca gacccaacat ctacgcttga attctacgac
    17041 gaggagatgg agcagtgtgc tgaaagttat gcgaccttcg tcatggaaga agtcgctaag
    17101 gcgaaacaga caacagttga ctctattgta atagtagaac agcggctcga cttctcaagg
    17161 tacgtcccag acggctttgg gaccggcgac tgtctcgtca ttgcggatgg aaccctatct
    17221 gttattgaca tgaaatacgg tctaggaatt ttggtagatg cgtatcagaa cccacagatg
    17281 atgtgttatg ccttgggagc gttggagctt ttcgatgtta tttatgacat tcaagaagtc
    17341 aagatgacca ttttccagcc aaggcgtgag aatgtgagta cgtacacact gctgaaagaa
    17401 gaattacttc agtgggcaaa agatgtgttg tcgcctgccg cagatttagc tttcaaaggt
    17461 gagggtgagt accaatgcgg gaaatggtgt ggattctgtc cggcgaagaa tagctgtcgt
    17521 gcacgagcag accataactt gaaactggcg caatatgagt tcaaaccacc agagcttctt
    17581 agtgatgatg aaatcgaaga aatcatcggt aaagtggatg acctggtttc ttggagtaac
    17641 gatatcaagg actgtgcttt gaaacttgca ctcggtggca aacaatggac tcatcacaaa
    17701 ttagtcgagg gtcgctctac tcgtaagtat tctaatgaca atgatgtcgc tgcggcggtc
    17761 atcaaggctg gatacgaccc atatgataaa aagttacttg gtgtcacagc aatgacgaag
    17821 gcactcggta aagagaaatt caatgaaatc ttgggcgaat acatcatcaa accaaaaggc
    17881 aagctgacgc tagtcgatgg ttcagataaa cgacaagcgg tcacagtaaa caacttaaac
    17941 gaagaattta aaccattaac ggaggaacaa taatatgtct aaaacaagca aagtaatcac
    18001 tggtacaaac acacgtctct catacttcca cggttgggaa ccagcatcca tcaacggggg
    18061 cgcagagaaa tattcaacag cagtcttgat tccaaaatca gataagagaa cgattgatac
    18121 cattgaaaaa gcaatcgatg cagcaatcga agagggactt gcgaaattcg gtgggaagaa
    18181 accaaacaaa gcagaaatca agctgccatt acgtgatggg gatatcgaac gcgacgatga
    18241 agcataccaa ggccattact tcattaatgc gaactcaacc actgcaccac aaatcgtgga
    18301 tcagcacgtt caaccaatct tggatcgttc acaagtgtat agtggttgct acgctcgtgt
    18361 gtcgattaat ttctatgcct ttaacgcaaa tggaaatcgc ggggtagcgg cagggcttgg
    18421 gaatatccag tttgtacgtg acggcgaacc actcggctca caagtatctg caagcgatga
    18481 gtttgaaacc ttggcggatg atgatttctt ggcttaatca ttaaaacaaa aacggccatg
    18541 gcagaagtgc tgtcgcctgt ttgattggag gaagaaaatg aaacatttgt cattggattt
    18601 ggaaacgtat agtagtgtcg atttatcgaa atgtggtgtc tacaaatatt cagagtcccc
    18661 cgattttgaa atactgctat ttgcatacag tgtagatggt ggtcccgtcg aagtggttga
    18721 tctgaagatg gacgaaacta tcccggctga aatattaact tcgctatcag atgaaacagt
    18781 gattaaacac gccttcaatg cgagttttga acgtgtgtgt ttgtcacgct ttctggggtt
    18841 ggatggcttt ttgaatccac gagggtggca atgcacaatg gtgtggggtg cgacattagg
    18901 tttgccgttg tcacttgtag gagttggtgc tgttcttggt ttggataaac aaaagctcac
    18961 agaaggaaaa gacctcatcc gatacttttg cgtgccatgc aatccgacca aagttaatgg
    19021 tggacgcacg aggaacttac cacaccatgc gccaactaaa tggtcagcat ttaagacata
    19081 caatagtcgt gatgtggaag ttgaaatagc tatccaagga aaactttcca attttccagt
    19141 gcttgattct gtttggaaag aatactggct ggatcaggaa atcaacgacc gaggaattga
    19201 gattgataag gactttgtgg aagctgccgt tcagttggat gaattatcaa aagctggatt
    19261 gaatcaacaa ttgcaagaat taactggact agaaaatcca aacagcgtgc ttcaaatgcg
    19321 gggatggtta ctagaccacg gacttgaaac cgagtcactc ggtaaaaagg cagtggccga
    19381 actactcgaa caacctattg atgaaaattt aaagcaagtc ctggtcttac gtcagcaact
    19441 cgccaagtca tccgtgaaaa agtatcaagc catgcaaaac tttgcgtgtt ctgataatcg
    19501 ttctcgtggt ttattccaat tctatggagc caaccgtacc ggacgctttg caggacgtgg
    19561 catccagctt caaaacttgc ctcagaacca tatgtcagat ttatctgaag cgcgtagttt
    19621 agtaaaacaa agcaattttg aaatgcttaa cattctctac ggcaatgtcc cgaacgtact
    19681 ctcagaactc ctccggacag cgtttgtacc acgtgaaggg atgaaattta tcgtatctga
    19741 cttctcagcg attgaagcac gtgtgattgc ttggctgtcg ggtgagaagt ggcgtcaaga
    19801 agtattcaaa aatggtggcg atatttactg tgcgtctgcc agtcagatgt tcggtgtgcc
    19861 agttgaaaag catggcgtga atgggcatct acgtcaaaaa gggaaaattg ctgagcttgc
    19921 tctagggtac ggaggatcgg tgggtgcatt gaaagcaatg gatgctcttg aaatgggact
    19981 aactgaggat gaattacagc cgcttgttaa ttcttggcga gactcaaaca ttaacataac
    20041 ggagctttgg tgggctgttg ataaggcagt aaagacttgt gtgaaagaac gacggagcac
    20101 agcgacgcat ggcatcaagt ttgaatacaa gtcgggtttt ctgttcatcc aactcttgtc
    20161 aggacgtaaa ttagtttatg taaaaccaaa gattggtgag aacaaattcg gcggtgaagc
    20221 tgttacctat gaaggtgtcg gtgctaccaa gaaatgggaa cgtcttgaaa gctatggtcc
    20281 caagtttgtc gaaaacatcg tgcaggcaat agctagagac attctctgtt tcgcaatgga
    20341 gaatcttcgt aagtatcgca ttgtcgggca cgtccacgac gaagtgatta tcgaagtgcc
    20401 aatggaatcg aaggttgaag acatctgtca gttgatgagt caaacgccta agtgggctga
    20461 aggcttgcta ctgaacgctg atggatacga gtgtgaattc tatcaaaagg attagtcaat
    20521 gtggagtcag aaatggctcc atattttttt caaatttttt tatcaaaaat aaggttcgaa
    20581 ttgggttctt tcttcgctta tagacgaagg caaaaaaatc aaaaataaat tcgagtcaga
    20641 gtttactttc gaccattttc ttcgcctgta agttagaagg ggcaaaatgc ctctcctgaa
    20701 aattctatta caggaggttc acaagatgaa cgaattgaaa gtttttgaaa atgcggagtt
    20761 tggctctgta cgcacggtga tgattggcga tgtccctcac tttgttggta aagatgtagc
    20821 atcaattctt ggctacacaa atccacaaaa agcattacga gatcatgttg atgaggaaga
    20881 taagacggtg aacgaatcgt tcagcgttaa tgggacaatg gctatcctta tcaacgaaag
    20941 tggattgtac tcactcatca tttctagcaa gatgccgaac gcgaagaaat tcaagcgttg
    21001 ggtgacaaat gaggtcttgc cgtctatccg ccaacacggt gtgtacgcga cagaagattt
    21061 catcacaaag tcaatcgaag atccagcatg ggccatttca gtgttgaagc agctacaaga
    21121 gaaaaaagaa atggtggcga tgcaacaaca aatgattttg gagatgaaac caaaagttag
    21181 ctactacgat ttgattcttc aaaacaattc tgtgatgtcg atttctaaaa tctcgaagga
    21241 ctacggtatg tcaccacagg caatgaacaa gttgcttcat gaactgggaa ttcagtacaa
    21301 gcaaggcaag atgtggttgc tttatcagaa gtatgccaat caaggctaca cacaatcaaa
    21361 aactcatgcg atagatgcag agcgcagcgt gatgcatacc tactggacgc agaaaggtcg
    21421 tttgttcttg tacgacttgc tgaaatcgaa gaagaacatc ttaccagtga ttgagcgagg
    21481 tgataataat gcctagacgt aaagattaca gaacatcagt ctatgtctgc tcgccgttct
    21541 caggaaatgt tgtcgtcaac cataaaaaca ctaagcgtta ctgccgtatt gctcttgacc
    21601 gtggctacat gccagttgcg gcacatctga tgtatccaca attcatggac gacgcaacgg
    21661 agcgtgatgt ggcaatgcac atgtcacttg ttctcatggg caaatgtgag gaagtctggg
    21721 tggtgggtga caccatctcc caaggcatgg cagttgaaat tgaacaagcg gagtattgga
    21781 agaaacatat caggtacttc gatgatgaga tgaaggaggt ctcaaatgct taagatgacg
    21841 ttattcacat ccaatcaggt gggtaattcc aagaatggat tgttcccaac caagcaagtg
    21901 gtgacagata aagaaacact tgttgccacg gtcaagctgg atcatgtgat ggctgagtac
    21961 aaaggcaact atcgcaaagg tgacaacttc atccagtctg atgtgattcc acttgattgc
    22021 gacaatgacc actcagacaa tccagatgat tgggtagccc ctttcgaagt ggcgatgcag
    22081 tttcctgatg tgacgtttgc ggtcagctat tcacgtaaca acatgaaaga caaaggtggc
    22141 aaatcagcac gtccacgttt tcacgtatat ttcccgattc ctgccacaac tgataaagat
    22201 gaatatgtgg aactcaagaa gcagattcaa agtgagttcc catactttga caacaatgcg
    22261 atggatgcgg cacgtttgct tttcggaaca ccaaactctg aagttgaaat ctatgaaggt
    22321 agtaagttga tcactgattt tctcgaagat gatctgtttg ctgattggga taatgcgcag
    22381 tcagagatta gcgaaggcag ccgtaattca agcatgagtc acatcgcagg aaagctcatc
    22441 aaacgctacg gtgcaacgga agatacgtat cacaagtatc tcgaacaatc ccaaaaatgt
    22501 aatccgccac tgccggagga ggagcttaaa gctatctgga atagcgcggt caactttggg
    22561 aagaaagtga aacaacaaga aggctacatt cctccagagc agtacaactc tgatttatca
    22621 cttgagccaa ccgactactc ggacttaggt caagcaacgg tcttagctcg cgagtatgaa
    22681 tgtaagcttc attactcacc gtcaactggt taccttgtct acaacggcag ctattgggaa
    22741 gagtcgaaac caaaagcgca aggtgtgatc cacgcattga cagaacgtca actggaagaa
    22801 tctgaaaccg aaattgagaa gcgtaccaag gaaatggtta gtaatggcgc gttcgatgta
    22861 cttgctagtg ttggtccgaa aaaggcagtc accatgttca acacagcgca acgtcatagt
    22921 tttgatttgt accaacacgc acaagagtat aagaagtttg ctgtcaaacg ccgcgacagc
    22981 aagtatctct cgtcagcatt aaccgaggca aagcccatgc ttgaaatcga gcaacggtta
    23041 ctagatgtga acgagttctt gctgaacaca ccaacagcaa cctttgattt gcgaacaggc
    23101 aaatcacaag accacaactc agaagattac atcacgaagc aaacggaatg tgccccgagt
    23161 gacgccaatc agcagatttg gttggacgct ctgaacacca ttttcgtagg tgaccaagag
    23221 ttgattgatt atgtccagat gattgtagga cttgcagcta ttggtaaagt ttacgttgaa
    23281 gcgcttatca tctcgtatgg tgaagggcgt aacgggaagt ccacgttctg gaatgtcatc
    23341 agccgtgtgc tcggtaatta ctctggcagc atctcagctg atattcttac aagtcaaatc
    23401 cgacgcaatg tgaaacctga acttgcggaa gcgaagggga aacgcctact catcgcagcc
    23461 gagcttgaag aggggatgcg actcaacaca tctaacatta agcagctctg ctcgacggat
    23521 gaaattgcgg ctgagaagaa atacaaagac ccattcaagt atgtcccaac gcatactttg
    23581 gtgctctata ccaaccacct cccaaaagtt ggagcgattg ataaaggaac ctggcgtcgt
    23641 ttaattgtga ttccattcct tgctacgatt gacggcaata aggacatcaa gaattatgcg
    23701 gactaccttt ttgaaaatgc cggcggagcc attttgcaat ggatccttga cggtgcgaaa
    23761 aaagtcatcg ccgcagactt tcatttaacg gttccacaag ttgtcgctaa tgcaatttct
    23821 gaatataaga cggcgaatga ttggcttggc catttcttag acgagtgctg cgagactgct
    23881 gttgactttg aagaaaaatc tggtgagttg tatgcagagt atcgtgcgtt ttgtacgcga
    23941 actggtgagt acattcgtag ttcagccgat ttttacacgg ctctggaggc gggcggattt
    24001 aagcgccggc gaactaataa aggaaacaag attgttggtt tgcaactcaa atccgagttc
    24061 ttggaatagt tgattttaaa ggaaagtgta acctgtgtag gtcttatata gaacttttct
    24121 taagggataa aaaattacca cctatataaa gttatgtatt gcgtattcac gggttacacc
    24181 atacttgcag attggagaat atctaaatga aatttaaaca gtggttacaa attggaatcg
    24241 gtaaacgtga tttacgattt gatgagtttt acgagtttgc catgtcggat gtcacgtatc
    24301 cttggaagaa atcattcgat aagcaagtcg cacatctgat gattcactgc gcagaaaagg
    24361 aatacttgga aatcttgcgt gatgcgtatt tcgagtattt gacggtgtgg ctatgagaga
    24421 aaagcaggtt gagcaagcac tagtgaaagc cgtcaaaagg gtaggtggta tttgcccgaa
    24481 gtttacatca ccggggcttg ctggtgtgcc tgaccgattg gtacttatgc caaatgggaa
    24541 acttggattt gtcgaagtga aggcgccagg gaagaagcca cggtctttac agctttttcg
    24601 tatgaaacaa ctgacagatt tagggtttca gtgtttcgtg cttgatgaga ttgatcagat
    24661 acctgagtta ctagaaagaa taggaggtga tgtctgatga agttcgtacc acataactac
    24721 cagaaatatg cgattgattt tatcaaagat cataaaactg ccgcccttct attggacatg
    24781 ggcttgggta aaacggtgac aacgctgaca gctattaaag acttgatgca tgatgacttt
    24841 acaatacaac gtgtcttaat tattgcaccg ttgcgcgtga cgcaatcaac atggccaact
    24901 gaaattcaaa agtgggatca tttgaaagat ttatcgtatt ccgtggttct tggaacacca
    24961 agtcagcgaa tggcagcatt acaaaataat gccaacatct atctgatcaa tcgtgagaac
    25021 ttggactggc tgattaacaa atcgggtttt acgtttgatt ttgatatggt ggtgattgac
    25081 gaactgtcgt ccttcaaaaa cttaaaagct aaacggttca ctagctttat gaaagtcaga
    25141 cacaaggttg accgaattgt tggattgacc ggaacacctt caagtaatgg tttgatggat
    25201 ttgtttgcgg agttcaaggt tctggatatg ggcgagagat tgggctatta tatttcacgt
    25261 tatcgagata aatactttct acctgacaag caaaatggca tgcagattta ttcttggaaa
    25321 ccaagggaag atgcagaaca agaaatctac gacaagattt cagatatcac gatttcaatg
    25381 aagtcagtcg atttcttgga tatgccagag cttgtgatga atgaagtgcc agttgatatg
    25441 ggactcaaag aaaagcaaaa gtacgatgaa attaaggcag acttggtgct acaattgaaa
    25501 gaagccacca ttgatgcagc caatacagcg gtgctttcga ataaactgct acaaatggcg
    25561 aatggagcaa tctatgatga gtttaatgtg agtcatcaca tccatgacca gaagttagat
    25621 gctttggaag atttgattga aggtacaaat ggcaaaccga ttctgattgc ctattggttc
    25681 cagcatgatc tggagcgtat caaggaacga ttcaacgttc gacagatcaa aacgtctcag
    25741 gacattattg attggaacga aggtagcatc cccatcgctg tcattcatcc ggctagtgca
    25801 ggacatggat tgaatttaca agctggtggt tcaacacttg tatggtttgg tcttacgtgg
    25861 agtttggaac tttaccaaca aaccaatgca aggctgtggc gacaagggca aagtgatact
    25921 gtggtcattc atcacattat tgcgaaagat acgattgatg aagatgtgat gttggctttg
    25981 aaacgaaaag ataaaacaca atcatgtttg attgatgcag tgaaagctag attggaggga
    26041 agcaagtgac accaaaagaa tacttgaaac aagcgtaccg tttgaataaa cgaattgacg
    26101 accggattaa agagaaggcg gagctctatc aactggcggt gacagtttct tccacaacat
    26161 tcgaagaaaa gatttcaagt ggcacacgga atactgaggc accatttgtt cgtaaccttg
    26221 agaagtgtta ccaattggaa gctgaaattg atgcagagat tgataagttt gtagacctca
    26281 aacgacaaat gcgtggcgtg attgatgaac tcacaaacgt ggacgaaaag attgtgttaa
    26341 tcaatcgata cctgttggaa gagagctggg acaagattgc tgaagatctt cacgctgacg
    26401 tgcggacagt ctttcgttgg catagcaaag ctcttgagaa cttgaagcta ccagatgaac
    26461 ccatagtcat ttgaaatgtg tcagtgaatg tcagtagcct ttagtgatat cattaagatg
    26521 tcaaaaaaga caaacgatac tcaagcaata ccagcaatag aagtatcgaa caaatactcg
    26581 aagcctttgt gggaaaaatc cttcaagggc ttttcttttg cccgaaagga tgtgacccca
    26641 cttgccaatg aaaccagcta acccctgtaa gttcccaggt tgtccgaacc gtacgcatga
    26701 tacttattgt gatgaacata aaacacttgc gaggaaacgt tacgagaaat acgagcgtga
    26761 tccggcaatc aacaaacgtt acggcagagc gtggaagaaa atacgggcaa ggtacgtggc
    26821 agcacatccc ctatgtgaga tgtgtctgaa ggaaggacgc tttacttcgg ttgagattgt
    26881 gcatcacatc aaggaattaa gtgatggtgg tacgcatgat gaaaataatt tgatgagtgt
    26941 ctgcaaatca tgtcactcga aaattcacat gactcgacga aacacaaaag ataaaacaat
    27001 catgtgaaaa ataacaaggg ggggaggggc ggtaattatc cctaaaccct tctaaactgt
    27061 acagcgcgct aggcccttac gcgaaaaaag ttcagttcaa acgggggata gccccaaaca
    27121 agaaaaagag gtgataaatt ttggcaaaag atggaacaca acgcggtggc agacgacctc
    27181 gttcagggga acgctcacag ccactcgttg ataaagttgc tgctggaaag aaagcatcgc
    27241 ggatggaatt tgacttaccg gatgttgaat tattaattgg tgaagatatt ggtgagggtg
    27301 tagagcttga gggcaatgat tatcctgaac caagtgctta cctttctgca aaacagaagg
    27361 atggcactgt gctaggcgct gatgaaattt ataactcaac gttccaatgg ttgaaagacc
    27421 gcggatgcga aacgttgata gccccacgaa tggttgagtc ctacgctcag aactttgcgc
    27481 gttttgttca atgtgaaaat gctctgagtc aatatggttt gctcggaaaa cacccgacta
    27541 ccaaggcagt catggcctcc ccatttgtgg cgatgtcgca gaacttccaa aagcaagcaa
    27601 atgtgacgtg gtatgagatt caacagattg taaaagagaa tgcatcagtg gaatacacag
    27661 gagatccgaa tacggatgcg atggaacgat tgttacgttc taagaatggt tgaaaggaac
    27721 agaatttatg atagagaagg ttaatccgca acatccagat aaagttgcag atcgtattgc
    27781 cggagccatt gtggacttgg catacgcaga agaaaataat ccaaagatag cagttgaggt
    27841 tttaattggt catggcatct gtcatgtgat tattgaaact tcaactgctt taaatttgaa
    27901 tgaaatcagc caagcgatta atcgtatcgc aggcaaaatt aaaaaagatg tggtgattgt
    27961 gccccaagat aaagagttgg ctgacaatca agcgactgaa attcgttgtg gcgataatgg
    28021 aatctttcgt gggatgccat tgactgatga acagttacag ctctcaaaaa ttgcgcgtga
    28081 cttatatcag gaccatccat ttgatggaaa gtacattctt gatggtgaac gcttgataat
    28141 ttgtcagagc cacgcaaaga caaatgagtt aaaacaaaaa tatccaaacg ctacagtcaa
    28201 ccctctaggt gattggactg gcggcccgga tgtggataca ggtgcgacta atcgcaagct
    28261 tggttccgat atggcagacg gagtaacagg tggcggtatc catgggaaag acttatcaaa
    28321 agctgatgtg tcagtgaaca tctacgcatt cttgaaagct caagaaactg gtatgcccgt
    28381 tgagttaagc tgtgcgattg gtgacactga gattgatggc gtgccttatt ctgaaatcgt
    28441 ggaagttgcg cgtgattatg tgaatgagct tggcgggttt gagaaactag cagaatgggg
    28501 gttgttctaa tgaacacaac agaacgtttt gagaaagttg atattgataa gctggtccct
    28561 tatgccagaa atgcccgtac ccacagtaag gatcaagtga aacagattca agcttctatg
    28621 cgtgagtttg ggttcataaa tccggtgatt gttgataagg attataactt gattgcaggt
    28681 cacggacgta ttcttgcggc taaagaagaa ggtatcaagc aggtaccgtg cgtgtttgtg
    28741 gaacacctaa ccgaggcaca aaagcgtgcg tacatacttg ctgataaccg actagcaatg
    28801 aacgccggtt gggatgatga aatgttggcg gttgagttat cagacctgca aggtgaagag
    28861 tttgatttgg atttgcttgg ttttgaaact gatgaacttg ataaattact caacggtaat
    28921 gatgatgccc aagatgatga ctttgatgta gatgaggaac ttgaaaagcc agccttcagt
    28981 aaaacagggg acttgtggtt gcttggtcgt caccgtttag tttgtggtga ctcaacaaaa
    29041 cctgaaactt atgagaaact catggacggc aaactagcaa atcttgtggt gacagatcca
    29101 ccttatgggg tctcatatca aggtaaagcg ggaacgatta aaaatgataa tctgaaggac
    29161 gaggcatttt acaacttctt gtatgatgcc ttttcttgta tggaaagtgt gatggccaac
    29221 gacgccagta tttatgtttt ccatgcggat acggaaggct tgaacttcag aaaagcattc
    29281 gtggatgcag ggttctattt atcaggtacg tgcatctgga agaaacagag tctagtgctt
    29341 ggtcgttcgc cttaccaatg gcagcatgag ccggtgctct atggctggaa gaagaaaggc
    29401 aaacatcaat ggtacaccgg acgtaaggaa tcgaccatct gggaatttga taaaccaaag
    29461 aagaatggtg accacccaac gatgaaacca attccgctgt tggcatatcc gattaccaat
    29521 tcgtcactca gcaactgcat cgtgttggat agttttggtg gcagcggatc aactttgatt
    29581 gcttgcgaac agaccgaccg gatttgccat acgatcgaac tggatgaaaa gttctgtgat
    29641 gtgattgtta agcgttatat cgagcaggct ggaagcgatg cagacgtttc agtgattcga
    29701 gatggaaaga ctatcgcatt taatgaccta gaaaatcctg aataaataac gtatcacttg
    29761 ctataaaatc cctttagagt gatatataga ctaccaaaag aatacggaag acaaagttat
    29821 gtttccaact catgaacaaa tcgagggtat gaaaaaacgt taccctgaag gtagtcgtgt
    29881 agaactcatc aagatgaacg acccacatgc cccagcgaag ggcacacaag gcacagtaac
    29941 cggtgttgac gacattggtt caatccttat ccaatgggac aacggttcaa cactgaacgc
    30001 ggtatacggc gaagatgtgg tgcacatcat caaaccagac ttcgaattgg tctttcaaaa
    30061 cggtagaaga gaaggatttg aaaccttcaa tgatgcttgg caatacgtga cggacatgat
    30121 attgaaccac gatttggtct gggttgacct tgagtcaaaa acagaagata ccatctacat
    30181 cagaaagggg ctttaagatg aaagtgagta aacgtaagat ttacaacatc gcaaagaatt
    30241 acatcgttca gttgcctgaa cgtggcgacc tgaaggccca caacagcgac cgtgaagact
    30301 tcttggacat tgcggtttgg tgtttggaag atgccttaat tgcggcctat gaggcaggca
    30361 gaaaggacgg tcaaaatgaa cgcaaagatt aagcaacaaa tcattgagat tgcagaaagt
    30421 ggtctgacaa atatgtttga tgcgattgca gtgcagcgaa ttgcacatga ccggcacttt
    30481 tacgagttgg tgattttcat tgaagaacac aaagatgagt acagccgctt tatcttggag
    30541 ggtgataaat gatggattat caaatttaca tgccagataa tgaagatggc attgaagaag
    30601 gatactacga ttggcaagaa ctggtgcaat tgctgcggga taacaagaac aaccctgaag
    30661 ccattcagtt cattgccgat atgatggaat aaagagatta aggcagctga caacagctgc
    30721 tttttgcgta gaagggagtg agttccaatc gataattaca aaccaactga atttatggac
    30781 aaagactccc attatgataa gtcacgtgca gattatgccg tggcctttat tgagttgttg
    30841 aaacacacga aaggtcagtg gttcgggaaa ccgttcaaac tgatgccttg gcaagaaaag
    30901 attatccgtg acttattcgg aacgattaag gcaaatggct acagacaatt caatacggct
    30961 tatgttgaaa tacctaagaa gaatggtaag agtgagttag cggctgccgt ggctttgctt
    31021 ttgacttgcg gtgatgggga agaacgcgct gaggtatatg gctgtgctgc tgatcgagcg
    31081 caggcttcaa tcgtatttga cgtagctgtt gatatgattc gccagaaccc agcgttaagc
    31141 aaacgagtga aaatcctgac ttcacaaaaa cgaattattt atcagccaac gaatagtttc
    31201 tatcaagtat tatcagctga agcatactct aaacacggat taaacagttc tgcggttgta
    31261 tttgatgagc ttcacgcaca acctaacaga aagctgtttg atgtcatgac gaaaggggca
    31321 ggggatgcta gaacgcaacc gctattcttt ttgattacaa cggcagggaa tgataccaat
    31381 tcaatttgtt atgagcagca tcaaaaagct ctcgacatta ttgaaggtcg aaagcatgat
    31441 aaaacattct atccgttaat ttacggtatc aagcaagatg atgattggac tgatccagaa
    31501 gtttggaaga aagccaatcc gtcacttggg ataacgattg cagaagaaaa ggtggtggct
    31561 gcttgtgatt cggctaaaca aaatccagct gaagaaaact cattcagaca gcttcgcctg
    31621 aaccaatggg tgaaacaagc agtgcgttgg atgccaatgg ataaatggga cgcttgttca
    31681 ttcaatgcag atgaagaaag tttgagaggt cgagtttgtt acggaggtct cgacctttcc
    31741 agcacgaccg accttacatc cttcgttttg gtgtttccac ctgaagatga ggatgacaag
    31801 tttgtgatac tgccatatta ctgggtgcca gaagatacct tggacttacg agttaaacgc
    31861 gaccatgtga attatgattt gtgggccaag caggggtaca tacaaaccac tgagggaaac
    31921 gtagtccact atggtttcat tgaaaaattt attgaggaac tgggtgagaa atacaatatc
    31981 cgggagattg cctttgaccg ttggggagca gttcaaatgg ttcaaaactt agaaggtatg
    32041 ggatttactg tagttccttt cggtcaaggt ttcaaggata tgagcccggc tactaaagag
    32101 ttgatgaagt tgactcttga acagaaatta gcccatggcg gtcatccggt tcttcgttgg
    32161 aatatggaca acattttcgt ccgaactgac ccggcaggta atatcaaagc tgataaagaa
    32221 aaatccactg agaagattga tggtgccatc gcgacaatta tggctctgga tcgtgcaatt
    32281 cgttgtggaa atgataatgg tgccagtgtt tatgatgata ggggattgtt gtttctgtga
    32341 aatatgagct tttattacaa tccattgata taattgtctt tggaggtgta tgtgtatgaa
    32401 tgaatatttg attcaagtag caccagataa aatatcttca gacccggaca acacattaat
    32461 ttctagactg ttttatagaa aatggtgggg aaaaattcta actgaagatt tgacaaagta
    32521 tgctgaagga attgggaatt ataggggaga aacgattatt tctttcaaca caattgctgg
    32581 tgcaacgata cggttattga aatgctataa tcaaaagaac ttttcaatgc cagatagtca
    32641 ttatgggcgt ttgaaagtca ttttatattc cgatgatatc accgaagaga tgaaagtgaa
    32701 atttgtaaaa ttctatgatt taagtcatac tcttgcaaat tttatgccac tatatgtatc
    32761 aaaaccttct ctcaatggtg ttaaaggtaa agggccgtat catgactttc ctgatttatt
    32821 ttattctgat gtaagagata aacgatatgg ttcatcaact aacgctgata aatttatcgt
    32881 taataataaa gactatttct ctaaattcaa agattggaaa agttttatag aactaaatta
    32941 tttacaagat ttcttcgatg atgaagatta tatgacgttt aaaaaacttg caccaagtag
    33001 ttcattcttt ccttatcaaa gaaaatcctt aacacctgac gaaaaatcga aatgtttaga
    33061 ggaagtaaat acctttttaa ataatgcaat tgagataatt ttgtcaagag gaagtcggtt
    33121 ttatatctaa tgtgaacaca gcatctacaa atcgtaggtg cttttttgat actcaaattt
    33181 tgaaaggagc aatcaatggg cataataaaa tcactattca aatcacgcga taaaccaaca
    33241 aatcaaacca tcatgaatcc ataccgattt atgtttggcg gctcatcagc tggtaaaagc
    33301 gtgaccgaac aaacagctat gcagatgacg gctgtgtact catgtgtgcg cgtgctatca
    33361 gaagccgtgg caggattgcc attacacttt taccaatacc gagatgctgg cggcaaagat
    33421 aaagcaatga aacattcgtt gtatttcttg ctgcatgatg aaccaaattc ggaaatgaca
    33481 tcatttgtat ttcgtgaaac cttaatgact cacctgctac tttggggtaa cgcctatgcg
    33541 caaattatcc gaaatggcaa aggtgaagtt gtagctttgt atcctttaat gccagataag
    33601 atgacggtta atcgcgatga caacaaggaa atctattatt tatatcaagc tgatgtcggg
    33661 caagtcagac tttcaaaagc tgacgtgctt cacattcctg gccttggctt tgatggcttg
    33721 gttggttatt caccaattgc catggctaag aatgctatcg ggcttgcgat tgcaacagag
    33781 gaatatggtt ctaaattttt tgccaatggt gccaatcctg gtggtgtcct agaacatcct
    33841 ggaactttga aagacccagg aaagcttcgt gaaagttgga atgcgacatt tggtggcagt
    33901 tcaaatgcgg ccaaggttgc agtacttgaa gaaggcatga agtatacgcc tatttccatg
    33961 tcaccagaac aagcccagtt ccttgaaaca cgtaagtttc aactggaaga aatcgcacgg
    34021 atattccgag tgccccttca catggttggc gacttagacc acgcgacctt ctcaaacgtt
    34081 gaacatttga gtttggattt tgtgaagttc tcattatcac catggctcaa tcgttgggaa
    34141 caagcactta ataaatcact gttaagtcgc gatgaaaaag aaaagtattt cataaagttt
    34201 aatgtggatg gcttattgcg tggtgactat gccacacgtc aacaaggcta tgcaaccggt
    34261 attcaaaatg ggttcctatc tcctgatgat gtgcgtgaac ttgaggactt gaacccaatc
    34321 cctggtggtg ctggtgcgaa atattacttc aatggcaata tgctgccaat tgaacaagcc
    34381 ggggcagctt atggcgtaaa caaagaagaa agcgaggaac aagatgaaga agttttggaa
    34441 gtggaagaat caaacggttc tgaatcaaga caaccaagag gaaatcctag agagaaccct
    34501 ctacctcaac ggaccaatcg ctgaggagag ctggtttgat gatgaagtca caccgcagat
    34561 tttcaaagat gagttaaatt caggtggagg taacgttaca gtctggatta attcgggtgg
    34621 tggcgattgt gtcgctgccg caagtattta caacatgctt gctgactaca aaggtaaagt
    34681 cacagtcaag attgacggca tcgccgcttc ggccgcaagt gttattgcaa tggcaggtga
    34741 caaggtactc atgagtcctg tggcgatggt gatgattcac aatccgtcag cggttgcgtt
    34801 tggcgaaaaa gttgatatgg agaaagccat tgccatgctt gatgaagtga aagcaagtat
    34861 tatcaacgcc tatgaacgta aaacgaattt atcgcgtgtg caactggcac atatgatgga
    34921 tgcggagacg tggatggacg caaataaagc tgttcaccta ggatttgccg atgaaatcat
    34981 cggtgagatt gacccggata ttccggttaa taattcgatg tactcggaag tcaaagtaac
    35041 taattcactg acttcagcga ttgctaagaa gtgtcgtatt gaaccacctg aaccaaagga
    35101 acgagaagaa attaataaca ctatcccagc cgattcgtta atgagtcggc ttaatttaat
    35161 gaaaaattgg aggaactaac ccatgaacaa aattcaagaa ttgatggaaa aacgtgcaca
    35221 agcttggaac ggagcaaagg cttttgtaga agcaaagcaa gataaagatg gcttgatgtc
    35281 tgaagaagat gccaagactt atgcggaaat ggaaacaaaa gtgaagaact ttacgatgga
    35341 aattgaacgt atgcagtcaa tggaagcgat ggatcgtgaa cttgaaaagc caatgagcca
    35401 accattaact tcgcaaccta tgactactga aaataaaatg gaacctaaga aaacaggacg
    35461 tgcttcagct gaatataaag aaggtgtact tgaagccttg cgctcaaact tccgacatgt
    35521 ttcaaatgtt cttcaagaag gtgtggatac ggcaggcggt tatttggtgc cagaagaata
    35581 tgactcacgt ttgattgacg gcatgacttc tgaaaacatc atgcgtacac ttgcgacaac
    35641 aattacgaca agtggtgaac acaagattaa tattgcaggt tctaaaccag ccgcttcatg
    35701 gattgaagaa ggtggcgagt tgagctttgg ggatgcaacg ttcgatcaaa tcttgcttga
    35761 tgctcacaaa cttcatgtgg caatcaaggt gactgatgaa ttgctttatg acaacgcctt
    35821 taaccttgaa aactatatca tcgaccaatt caccaaagcc ttagcaaacg cggaagaaga
    35881 tgcgttcttg aatggtgacg gtgttggtaa accactcggt atttttgcag caacaggtgg
    35941 cggacaagtt gccattacaa ctaatactca aagtgcagtt acggcagatg atgtggtgaa
    36001 ccttgtgtat gctttgaaac gtccatatcg taaaaacgcg aagtttatca tgaacgacca
    36061 aaccatcagc ttgcttcgta agttgaaaga caacaatggt gcctacatgt ggcagccagc
    36121 tttggtagca ggtgaacctg accgtctgct cggttatcca gtttatactt cggagtttgt
    36181 tccaaccgtt aaggcaggtg cgccagtcat tgcttttggt gatttcagct actataacat
    36241 tggcgaccgt ggcgtgcgtt cattcgacca gttgcgtgaa ctctttgcag gtaatggcat
    36301 ggttggtttc ttagctaaag aacgtgtgga cggtaaactt gtgttacctg aagcagtgca
    36361 aatcttgaag atgaaagcat ctgcctcatc ttaaggagta gcctatgagt gacttactaa
    36421 ataaggtcaa ggcaaacctc atacttgagc ataacgaaga tgatgagctg ttggaacgat
    36481 tgattctgac cgctctttct tatgctgaga gctatcaaca tattgagtct ggctactatt
    36541 cagaacatga aatgccgcca accactgaac aggcggttat tgtgttagcc agtaactttt
    36601 acgaaagccg tgatggttca actggtggct tctttgcgga taacgtcaat gccagtaagc
    36661 aagtctggga aacggtcaat ctgttactca ggctcgatag gcggtggcaa gtatgagttt
    36721 tggaaagatg aatcaacgaa tacaaatcgt taaagtgacg aaccaaaagg atagcgctgg
    36781 attttcagtg aaacaagatg aagttcttgc agctgtccat gcatacaaag aggaaaagaa
    36841 tgcgactgag aaatgggtaa acatggcgac ttttagttca gcgacctgcc tgtttcggat
    36901 tcgttatatt cctaatctag aggttacgac agagatggtt gtcatttcag atcagaaaag
    36961 gtatcagatt gtgtcggtgg agaatgtccg aaatcgcaat atgtaccttg aaatgctgac
    37021 tactgaaatg gaggtgagtg cggatggcta aagcattgat gaagatgcca gaagatttct
    37081 tgttaaaggt ttcaaaacta gaaaacaaga cagatgaaat catccccaag gttttagaaa
    37141 gtggtgctga agtggtggaa ggcaaagtcc gaagtaactt aactgccgtt gttggctcaa
    37201 atacaaaagt accaagtcgt tcaactggtg aacttgaatc agcccttggt atcacacaag
    37261 ctcgtcaaga taaaagcggc aactggaaca tcaaggttgg ttttgatgag cctcgaatcg
    37321 atggcagttc aaatgctaag attgcgaaca ttctcgaata tggaaaacac ggtcagccag
    37381 caaagccatt cttgaaacct gcaaaatcac aaagtcgcaa gtcatgcatt gaaacaatga
    37441 aatcaaaaat ggaaagtgag gtaaacggca tttgaacatt ttagaggaat tgaacagtct
    37501 ttttgccaca cttcatattc ctgttgaaac gggcgagttt agtgataaag cgccagacaa
    37561 gtattgtgtg ctgacaccac tcgctgacag gttcgatgtt tacggggata actttccaac
    37621 cgttgatgta caggaagttc gcatttcctt gtttgtgaaa ggcaactatc tgaacacgca
    37681 aaaacaactg gttcagaact tgttgaaaca cgagtttacg attactgaac gattatttgt
    37741 aggatttgaa aaagatacaa attactatca tgtggcgatt gatgtcgcca agcattatga
    37801 aatggaggtc tgaaaatggc aacgattgga ttggataaat tatattatgc accgattaca
    37861 gagtcggcaa ctactggcga agaaacatac ggcacacccg ctatgcttgc gaaagcaatc
    37921 tcagctgagc tttcggtgga acttgctgaa gcaactttat acgccgatga tggtgcatca
    37981 gaagtagtga aagaatttaa aaatggaaag ttaacgctcg gtgttgatga tattggacgt
    38041 gctgtggcgg cagtgcttgt tggtgcaacc gtggatcaaa acggtgtctt aatttcgaca
    38101 accgaagatg gtggcacacc agttgcgatt ggtttccgtg ctcgtaaatc gaatggcaaa
    38161 tacaaatatt tgtggttgta ccgagtgaag tttgccgtgc ccagcacaag cctagcaaca
    38221 aaaggtgacg acattacatt ctcaacacca acaattgagg gtacagtcat gcgtcgtaac
    38281 aagttagatg ctagtggtaa gcatccatgg aaagcagaag ttgatgaaaa cgatacggga
    38341 gttgaacaaa caactatcaa tggatggtac acgagtgttt atgaaccaga tttcacagca
    38401 gactaggagg caacaatgag tgaagaacgc agtgccaaga ttagccttgg cggagatgaa
    38461 tatgaactga ttttgacaac aaaagccacg aaagaaatcg ccagtcacta cggtgggctt
    38521 gataaccttg gtgagaaatt gttgaaatcc gagaactttg aattggcgct tgatgaaatc
    38581 atctggttga ttacgctgtt ggcgaatcag tccatcaaga tttacaactt gaagaacaca
    38641 gaaaagaaag atgaactcac gactgaatat gtggagttga tgacgagtcc gttgcagttg
    38701 gctgaatata aatcagcaat cactgaagca atgttcaagg gcacagctcg caatatcgaa
    38761 agtgaaacag attcaaaaaa caaagcaggc gagtaagtga tgaagaaaca tttactcgcc
    38821 tgtattacta tgggacagtt caaatgggta tgacgtcaga tgatttctgg tactgctcgc
    38881 ttggactgtt tttggattta tgggagtgtc ataagcaatt tactgggatt agcaagccga
    38941 aagttgagtt attcattgat gatgtgatac cgagtggaat ttaagggaag gtggtgattt
    39001 atttggcaga taattttggt ttaaagattg gcgttgaagg tgaaaaggag tttaagaact
    39061 cgttgcgtga aatcaaccaa tcattcaagg tgctaggttc agaaatgaaa ctggtatcat
    39121 ccgaatttga taaaaacgat aaaagcatca gctcactgtc tgctcgcaac caagtcttaa
    39181 acaaatcgat tgatgcccag aaagaaaaaa tcaccaccct tgaaaatgct ttgaagaacg
    39241 ctagtgacag ttttggtgaa aacgataagc gtactcagaa ttggacagtg caattaaaca
    39301 atgcaaaagc ccaactcaat aatatggagc gtgaagttgc taacaacgag aaggcaattg
    39361 acgccatggg tactgaagaa gttgatgctg ctaagaaaac tgatgagctt ggcaacgaac
    39421 tcaaggatac aggtgacgag gctgaaaagt ctggaagtaa atttgaaaag ttcggtggcg
    39481 ttctaaaagg tatcggggta gcaatgggta cagtggcact tgctgctggc gctgctgccg
    39541 tggagctagg aaaacaagtg gtatctgctt acgcggacta tgaacaatta gtcggtgggg
    39601 tggatactct tttcaaggat gcgtcatcca ccgttcaaaa gaatgccgac aatgctttca
    39661 aaactgccgg aatgtcagcc aatgaataca tggaaactgt cacaggtttc tcagctgcct
    39721 tgatttcatc tctcggtggt gataccaaga aagccgcagg agttgcggat caagctgtta
    39781 ctgatatgag cgataatgca aacaagatgg ggacggatat tgagtccatc cagaacgcct
    39841 atcaaggttt cgccaagcaa aattacggga tgctggacaa cttgaaatta ggttttgggg
    39901 gcacgaagtc cgaaatggaa cgtttgctcc aagaggctga gaagatttct ggtgtcaaat
    39961 atgatatctc gaacttcgct gatgtgactg aagcgattca cgttattcaa accgaaatgg
    40021 gcatcactgg tacaaccgcc aaggaagcga gtgagacgat aagtggttcg attgacggta
    40081 tgaagtctgc aatctcaaac ttgatggctg gacttggtaa cagcagcgct gacattggta
    40141 tgttgattgg caatgtggtg gaacaatttc aaaacgtcat caagaatatt ctaccggtca
    40201 ttcaaaatat cgtgtcggct ttaccggcag tggtggatgc cattattcaa gcattggggc
    40261 agttattgcc aacactctta gaagcagtaa cctcattatt ctcacaagtg cttgaaacga
    40321 tacttggctt acttcccaca cttattccag tggtggttca agccgtttta acgattgttc
    40381 aaacacttat tgagaactta cctttactga ttgacgctgc ctttcaactt gtgacaacac
    40441 ttatccaagg actaggcgaa gcgctgccag aacttattcc aattacagta caggcaatca
    40501 taacgattgt gaacggcttg attgaaaact tgccattact gttggatgct gctttacaac
    40561 ttatcatggg acttgcccaa ggacttatca cagcacttcc aatactgatt gaaagtctgc
    40621 cacagattat tacaggaatc gtgaacttta ttatcagtgc tattccacaa atcattcaga
    40681 ctggtattca acttttgaca tcacttgtga cagctttgcc ggatatcatc aatgcgattg
    40741 ttgcagctat tccagtgatt attcaaaata tcatcaatgc cgtgattggg gcaatccctc
    40801 agttgattga cgcaggtatt cagttacttg tggcattgat tggtgcgtta ccgcaaatta
    40861 tcacaacgat tgcgaatgcc atgccgcaaa ttatcaacgg aataacagga actttggtaa
    40921 acaacatcga caaaatcatc aatgctggtg tgaagttgct tgtggcatta gttcaaaact
    40981 taccagcaat catttctgcc attgtcaaag cagtaccgca aatcatcaca gggcttgcga
    41041 atgctttcgg tgagtatgtt tcaactatgg gaactatcgg gaagaacttg attacaggat
    41101 tgtggaatgg tatcgctgat gctggaagtt ggttacgcaa caagatttca ggattctttg
    41161 gcggtgtagt agacaacatc aaggacttct ttggtattca ttcaccatca acactgtttc
    41221 gtgatgaaat cgggaagtac atggcacaag ggattggcgt aggttttgat aaggaaatga
    41281 ctggtgtttc taaaacgatg cagaaagcta ttccaacaaa actttatatg ccaagctttg
    41341 atattgatac cgatatccac gctgccgttg atagcacatt ctcgatgctt gatttaggtg
    41401 ctaaggttga tgccttaaca aacatcacga gtgaaatgtt gccaacgcta cttaaagcaa
    41461 tggacattaa agtcgtcttg aatgatggaa cattggtcgg caaacttgct ccagaaattg
    41521 ataagagctt ggcgacgttg aatcgtagac gtgtagcata ttagtgttgg attttcacat
    41581 tttgactgta tactataact aacgagtgtt tactgaagag atggtggtaa tcatatgttg
    41641 aagcataaat ttaaagcaaa gaaacctgca ctgataggat atattctact gcttgctatt
    41701 ggattatttt taacggcagt aagatggtat agcggcataa atcatggttt tgtattcatc
    41761 aacaatcaga ttaattctca catttcaaac ttcgcattaa gctttctttt ctatttggtg
    41821 ataggctatc tttggttgct tatgggaacg aatttaaggg ttcttactgc gatgggagct
    41881 ggtctgattg ttgcaaatat tgtatgtgaa acgatgatga cttttctgaa tacaccagat
    41941 ctaatcgatg caatatatgg aatttccggc gtgctggttg tatacgcgta tttagcgatt
    42001 accaagaaaa tagggctgga agaaacaacc gaataggatg atagattata aactttagga
    42061 ttagacattg ttgtctggtc cttttttagt tatttagatg ggcggtggtc aattgaacgc
    42121 atttatttta gacggaacaa ttaattcacg gactgattta ggtttgcgaa ttacggaagt
    42181 gccagttatt ccagtgacgg aacgtgtcat tgatagtatt gagattgatg gacgtgaagg
    42241 agatttgacc attctgaaag gctggaaaga tgtgactttc ggttttaagg tagtgatttg
    42301 gaaatctgat gtttggacta gctggcagat aattttgcca caaatcctaa acgctaaaac
    42361 tatcgcattt tcaaatgaac caaacgtgtt ttacccaatc aagtatgcta aggcgagcgg
    42421 actaactcag attttatctt ccatgtggga gtttgaaatg gagttaacct gttcgccttt
    42481 tcgttataag accaatgtag cgactatcaa tcgaaccacg tcaggaacgg tcaataatcc
    42541 cggcaacatt tactcattgc cgaagattaa ggtctacggc aatggaacta ggacactcac
    42601 gataaatggc aagcctatcg tgttaaatct gacgaatggc aacctgacac tagattcgga
    42661 tttgaaagaa tgcttttacg gggatgtggc agccaatcaa tacatgacag gtgactttcc
    42721 tgagtttcga gtcggcagta atactgtgac tcttggtaca ggtataacca aagtagaaat
    42781 agaaccgagg tggcgatact tatgattacg ctttacaaca aaacagaatc aaatttcaat
    42841 cataacggtc tcgctgtcct tgatggcaac attctgaacc ccgtggtgag tgaggaattg
    42901 aacggactct tttcctttga attcgattat ccaattcatg ctccacacag tgaggaattg
    42961 ttgccagaaa tgattgtgaa agcaccagta ccagaactgt ccgaccagct tttcaggatt
    43021 gttgaacgag atgatgcact tggtggtttt ttgcacatag tagctcatca tattttctat
    43081 gacctagcca caaactttat tgaagatact tatatcgtca acaagaacgg gtcgggagct
    43141 ttgacacaac tgcttggtgc aactcaattc atgcaccgtt ttaccggtac atcaaacatc
    43201 agtgcggtca ataatgtccg cttggtgcga ctaaatcctg tagaggtgtt gcttgatagt
    43261 gacctagaga atggttttca aagcagttat ggtggtgaaa ttgtacgcga taacttcacg
    43321 atttcaatgt taacgactcg tggcagtaac aacggcgtac aaatccgaga caagaagaat
    43381 ctgacaggat ataaagctga tgttgattac tctggaattg ttactcgcat tatgccacaa
    43441 gggtatgatg gactatttct tcccgaaaaa tacgtggaca gtccaaacat caaccaatac
    43501 gtccagccta aaatcaaagt catcaagtac gacaacgtca aagttggtga tgaagaggat
    43561 gagtttactc aagccgaggc ctatgatgaa cttcgcagat tggctgggtt agaatactca
    43621 gctaacaagg tcgatttgcc aaaagccacc tatgatgtgg agtttgcccc gcttgagcga
    43681 actgaagaat ataaagattt tgcacagctt gaaacgatta atttgggtga tactgtttca
    43741 gttattcatg ccgaagatgg attgaacatc acagctagaa tggtctcata caaatacgat
    43801 ccatttctga aagcgtatat ctcagtaaca ctcggaaaca tcacgccaaa attcacggat
    43861 gtggcgaagg acatcaaccg ggtggacaac aaggcggagc aagccaaaga cgatgccaat
    43921 tacgcattga gttcggcaaa tggtaaaaac accaacttct atggcccgac aactccaaat
    43981 aatccgaaac tcggtgacgt ttggtacaaa gaaaatggtg acaagtttga aatgtgggtg
    44041 tatgaaatcc gtgatggcgt cactcagtgg tatccactta tgacagatat gacggcagaa
    44101 gaagtaaagc aagccgtgga acaagcacaa aaggatgcca atactgccac tgaaaaggcc
    44161 aatcaagcat ttaacgatgc tgtgaatgct ttgaatgcag ctgaaagtgt aggtgcaagt
    44221 gttgaaacgc ttactacatc cgttgaacag aatactagca agatttcatt agtggcgagt
    44281 ggtatgtcaa ctcttgaagg acgagtaatc acagctgaat caactctgac agtgcaagct
    44341 gggcagattg ctagtaaggc aagtcaatcg acggttgata atctgacagg gcgtattaca
    44401 actgcagaaa gcaccatcac ccaaaatgct gatagatttg agctatctct ttctaaaaca
    44461 aataaagcgg tcaatgtttt agccggtatg agcattgcag atttgcagtg ggcagttggt
    44521 ggtctgaata caaatgatgg aaaagaaaat acctatggtg gatacgttcg ctcaggtttt
    44581 acaagagtta gaaatggcga aaagtatcta cttcaaacat ttgtgggtgg gaaactttac
    44641 tctacttacg caacggctta tctgctttat tacaaagagg ataagtcctt tttatcgtac
    44701 acccaatttg gaaacaccac cacaccgctt actgttccaa gcagtgccgc ttttattcgc
    44761 attcgtttaa caactagtga tgcaccagaa accatcagtt gctatatgtt gaaaacggat
    44821 gtgacaaatg gctatgttga tttatccgag ataagtaacg tcgtaaaact tacagcgacc
    44881 actgatgcct tactgttaag tgtttcagaa accaaagaag caatcggcgt tccatttaaa
    44941 gtcaatcaat gggaacaagg aagtttgagt accactgacg gttcagaagt gtccgcaacc
    45001 aattatctcc gaagtgagtt ccttgatgtc aaagtgggag ataagtatat cgggcaactg
    45061 cgtgacggaa ctcaagctgc tttctactat cattactacg gcatggattt accttacatt
    45121 gattttgtcc caacggcaaa acaatttagt gaaaccctaa cggcgggaac ttatactaat
    45181 gcgaccattg cgagttatct agtaagtaag aacgttgaat cgatgagtat tacaagtggc
    45241 gtgacaacaa acccctattc aaattcagga gattatctgg tcatttacaa attgccattg
    45301 gaaggcaaag tcgctccgag tgtaatttcg tggaatggtc gcaaggatac atcagataat
    45361 attgtcttgc tttatacagg tggtgcttgg gaacagattt cgaccgttac aagtagcagc
    45421 aatgccactt attcttggac gttaacggct gagcagatat cagggattac tactgacagc
    45481 gtgtatctag ctttcttttc agttaagact ggcacatatg ctgggattta taattcaagc
    45541 aacacacctt ttactctaaa cccgtcggat ggttcaagtt atcatcaaat ctcttatact
    45601 ggttcgagcg gagcaattac agtcccgagt ggcacattga aaatgcgagt ccgagtgaac
    45661 acgagcatca aacccgatga atatgaagga aatatttatc ctacgaccac aaggcaagac
    45721 tataccaatg ccaacacatt atattcagca attctcatgc agaaagactt aatcaattta
    45781 cgagttggta aaggagacgt gattaatcaa atcaatatct cacctgaaag tattttgatt
    45841 gctggaaaca aggtgcacat cactggtcaa acaaccattg atagtgcagt gattagttct
    45901 gccatgatcc aaaatgcggc catcacaaac gcaaagattg cagatgccac aattcaaagt
    45961 gcaaaaattg caagcattga tgcggcgaag attaccacag gaactttgtc ggcagcccgt
    46021 attggcgcga actcgattac ggctgataag attgctgcta atatcctaac agcattgact
    46081 gcgagttcgt ccattcgaat cacaggaact acgattggat attattcagg aagcactctt
    46141 gtgactgaaa taaactcgca gggaatgacc attcgacgtg gtggaacgac agtcggaaca
    46201 attggtgcaa ataacatcag tggtcactct gattggcgtg gattggtatt tgatcttgag
    46261 tatggaacag agtatatgtc atgggctcat aaagattcct cctctgcgag tgtctacacc
    46321 cagaagcttt cttggtatgc gagaacattg cagtctggta tggagaaggg gttccacttt
    46381 gacgacgaca tttatttacc atcaatgctt cgtataggga cacaacatca aatatctgtt
    46441 ggtgggatta catttagtag ttccctttat actaagattg gctcaagttc cggcagcgct
    46501 ggtattgcat tcaacggttc taatctgatg ctcggagatg atgggaagtg ggttgacttt
    46561 ggagtcatcc gtgaaatctg tgccaaactt gcgggacgaa caatcgcttt accaaccggt
    46621 ttcagtagca acggtacagc gagtggttgg tacaatccgc agtcgtttaa ttcaatgacc
    46681 acatacagtt aggaggccgc catgaacgaa gaaaaagaag aagtgatagt aactgaacct
    46741 atcgaggcag atattccaaa gccaatgaaa gttgggacgg atatggttga tttgcttgtg
    46801 atggtcgtgg atgatctttg tgtccagatt ggtgatttaa aggagaagat agataatgct
    46861 caaaattaga aatgaagatt tggtggacgt gatgagtttc ttaaaaggtt ttgacttacc
    46921 accaaagatt agccgagtgc gaacgaagct tgcaaaactc atccaggtga aggttgatga
    46981 actctatcaa gacgaggtag acttgcttca aaaatacgga aagaaagatg agcacggcaa
    47041 tttggtccaa catgacggtg ccttttcact catcccagaa accgcaactg aatatcacaa
    47101 agaaaaggca gacttgctca acgaagaaac ccacatcaac gtggatgaac ttcaagacaa
    47161 gctgccttta ttggttgctg gatttgaaaa tagtgaagtc accatttcag ggaaagatgc
    47221 tgaaatgctc gatttaattt tagaacaatt ggaggatttg taaatgaaag aagtgtggaa
    47281 ttggattcaa ctcgccatcg caggtggtgg aggagtgatt ggatggtatg tgggaggaat
    47341 tgatggtttg atttatacgc ttgtaacttt tgtggtagtg gactatgtaa ctggtctgct
    47401 tcgtgcaatc aacgaaaaga aactatccag caagattggg gcaaagggca tcactaagaa
    47461 aatcatgatt tttcttttgg ttggtgtggc tcatatgctt gatgtggaag tcatcaaagg
    47521 tggaaatgta ctgcgtgatg cggtcatttt cttctatatc tcaaacgaag gaatttcact
    47581 gctggagaat gccgtggcga ttggactgcc agtgccggaa aagttgcgtg aaatcttgca
    47641 gcagttacac gaaaagaagg aggaaacaga aaatgggaat tagtaatcta gcaacaaaat
    47701 atggatttca gtcatttagc cattactcga atggtcgtag tggtgccaaa gtagataaga
    47761 tagtgattca tcacatggca accaccaact atgatattgt tcctgatatt tggaaaacgc
    47821 gtgaggcgtc agcacactac ggtattggaa aaaatggaga aattcgagca tacgtggacg
    47881 aaggaaacac agcttggcac gctggtaatt ggaacgccaa tatctcaagt atcgggattg
    47941 agcactgcaa tagcactgga tcaccgaact ggggtatcaa tcaagcaact attgatgcaa
    48001 gtgcaaagtt gtgtgcagat attgccaaac gttatggtct agggaaattg gtggtgaaca
    48061 agaatatctt ccctcactca catttttcgg ccacatcatg tccgggacag ttgcttggta
    48121 aactgcagga gattgcggat aaagccaatg cgattaataa tggttcatca accccaagca
    48181 cgccagctag tggtctgtat cgtgttcgca aatcatgggc tgacgccaag tcacaaattg
    48241 gtgcctataa ggacttgaac aatgcgaagc gggctgcaga tagccattct ggttataagg
    48301 tgttcgacca gaacggtaat caagtttatc catctgggtc atcaacatcc aacgtgaaaa
    48361 ctcttgtggt tcaagtgagt ggtctcaacg ttcgcaacaa gccaagttta agtggtgcgg
    48421 ttgttggttc atacaataaa ggcgcaactt ggacgcttcc gaagtcagag aagatgacga
    48481 ttgcggatgg ttgggtatgg gctcgttgcc cgattggata ttgtgctgtc ggtaagaata
    48541 ctggtaagac ggaatctgac gattatattt tgatttcata aatttaatcg gaacaaaatt
    48601 atgtccggat tgttgactta aaatatcgaa cgtgttatta ttaattcaac cagaacataa
    48661 atttgttccg attgaattat agaaagaggt atcgatatgg ttacgtttaa agatttgtta
    48721 ccagaaccaa cagaaagcca acaagctttc attttagatc acgggaaaac agaagatgat
    48781 ggtcagttga aatacgctga tgatgctaaa agttacggtt ggaacatgcg ccaatatggt
    48841 aaattgaaag ccggcgcggt tgttttgaat cgtcatccgg ggaaaattac taaagaccgt
    48901 aagtgggaaa tttacggggg cggatacgtt gagagcattt ctgaacctga tgaagctgga
    48961 aatgttacgg cagtaattac acacgcattt acaatcgaac cggcaattaa acaaggtgat
    49021 tcatttattg aaaacttcga ttggaataca cctagcaaaa agaagcgtaa aaagccgaat
    49081 tcatgggcct acttctggga tcagtatgga atgaatgaaa ttacctatgc cgattttgtt
    49141 gcattgattg aaaatcgtca tcttatccca atcgatgata cacaatcgtt gccgtctgaa
    49201 ctggatctta caaacgagga agttgaagaa attgagcaag aaagttcaaa aggatttact
    49261 gttcttgttg atgaagtggg accaaatcgc acgaatggga ctcagaaacg aaaattcact
    49321 ggacgccata ctgattggga gcgtgtcaac aaggcaaaac aaaaaacggg tgctctaggc
    49381 gaagaaatcg tacttgattt cctcattcag gaagcagaaa agaacaaaac aaaacttcct
    49441 gaacatgttt ctaaaactga aggagatggt catggctatg atatttgtgc gtttgaccaa
    49501 tcaggtaatg aaatccatat tgaagtgaaa gcttcaaaaa caaacttctc tgacggattt
    49561 gaaatgagta ctaatgaggt tgctgcctcc cttggagata caccatataa aatttacttt
    49621 gttcacgacc ttgatgtggc gtcaaaagtt tgcaaaatca aaatttatga tggcccaatt
    49681 actgaagaaa acttcacgat ggttccaacg agctacaaaa ttttcaataa gtagtttgac
    49741 gtctttataa ttcaaaacag cctcacaagt tttattgtgg ggcctttttt tcgtctagtt
    49801 ttctttaagg taggtgttta cagcattttg cattaggatt aaatgatttc ttaactgaaa
    49861 tacctgctct ttgtaattga tgcagaaaag aggcgattct attgattcca tacgattatt
    49921 catcgcgttt atattggctt gtaaaaactg gtataaagct tcttcagcct ctatggtttt
    49981 aaagcacttt tcaaattctt gtttgaagct ttcatcaaat tcctccagtt cctttgttgt
    50041 cgcagatttg caaaaatgtt catattcttt ttctaaccct tccatatcaa aactactcat
    50101 aactttctcc ttcccacaat tgatatttat attgttattt attggatgaa gcttgtaaag
    50161 gcttatttga tgtttgggaa gtaaaaagtc aaaatttaca aagaataatt cctactatat
    50221 ataaacaaac ttagaagcct gcaagagtga ttcgtttcat tcttgcaggc ttttttcgtt
    50281 ttgtgggttc gagtcggtta tttctttcgc atatggatga aaggcaaagg aaaatttgtg
    50341 attgggttta ctttcatgga ttttcttcgc ctgtatcttg aggaggcgaa agcaatgact
    50401 aacaagaaaa cagaaattaa ctattcagat gaagcagcct attacatttc acagcagatc
    50461 cttctgaact tacttgttac cggtaaaatc aacctggaag aatacgtcaa aatcgacaat
    50521 aaaaaccggg aaacattcaa tcctttttta tcaaggataa tggtctgaaa cgttgatata
    50581 acggcacttt agagctaaca tgtcacctaa tggaaaggag ttgagctgat gaaaacgata
    50641 cataaattgg cacgtccaga gttgccggaa gcttccaaac taaaggtggc agcatacgct
    50701 cgtgtatcaa catcaagtaa tgaacagctg gcaagtttac aaacccagat tactcactat
    50761 gaaaatcaca tcaagaacca tgaacaatgg gactatgtcg gtgtttatta tgatgaaggg
    50821 atttctggaa ccaagactgt aaaacgtgat ggtttaaaac gattgattaa ggatgcagag
    50881 cttggaaaga ttgatttgat tctgacaaag tctatttcca gattttcaag aaatacagtt
    50941 gactgcttga atctggttcg gcagctaact gatattggtg tcaccatctt ttttgaaaag
    51001 gaaaacatta atactggtga tatggaaagc gagttgttgc tttcaatttt atcaagcttg
    51061 gcagaaagtg aatcctattc ccattcagaa aacttgaagt gggcgattcg aaaccggatg
    51121 tctaaaggga aattcaaagc ttacccgccg tatggctaca agcggaaagg ctatgatttt
    51181 cacatcgttg cagctgaagc aaaagttgtt aaacaaatgt tccagtgggc attagatggc
    51241 atttcgactg gaatgattgc caagcggtta aacgatggaa aacaattcac taaacgtggg
    51301 gcaaattggc aagcaacatc agtcagaagt attttgaaaa atgaagttta taccgggact
    51361 ttgacgatgc aacaaacctt tgttgatgac cagtacaaac gacgcataaa caagggcgaa
    51421 ttgccaatgt accagatcaa aaacaatcac gaacccatag tatctaaaga agattacgac
    51481 cgagttcaag aactcattcg gagaaaagct gaggccaaag ggaatactgg tgacgggaaa
    51541 aagtacacca agcgatacgc attttcaggc aacatcatct gtgataaatg cggcagcaaa
    51601 ttcaagcggg ttcacactta ttggaaaggt aatacgaaga aggttcagtg gaagtgtacg
    51661 gggcatctta aaaataaaga tagctgcgat gcattaccaa ttacggatga agatttgaag
    51721 gttgcttatt tgaccatgtt gaataagttg attttcggac acaagttggt tctggaacct
    51781 tttatgaagt ctttgaataa agcaggtgcg aatcaggaag ggctcgaagt gcttgctgat
    51841 gaaatcgaca agattcaaga aaagcttgag gttctggcaa gtttgactgc ttccagtatt
    51901 ttatcaacga aggtgtcttt agaagaacaa agcaaacttc aacaagagag ggctgtcgca
    51961 caagaaaagc agcgcctcat cattgagtca gtcaacggca aaagcactca gcaaattgaa
    52021 ctagaagcct tatacaaatt cgcgcggatg gcagagatgt tgacggactg ggatgaagat
    52081 ttattcaaga agttcacaaa cagcattaca gtttacaacc gccaagaagt cggatttgaa
    52141 cttaagtgcg gactactttt gaaagagagg ttaccagaat gaacaataca attccatacg
    52201 ggtatgtcat taagaatgga gaaatcaacg tggatgaaca gcaagctcaa caaatacgtg
    52261 agttcttcaa tatttacctt gagggtgttg gcttacaaga agctggcaaa cgctctggca
    52321 ttaacaagcc gcatcccttt atgaaacgta ttttgatgaa tgaggtttac ttaggaaatg
    52381 agatatatcc aaaaatcatt gatgaggata ttttcttcca agcggatctg gagcaagaac
    52441 gaagaattaa gttctttaaa aaacgcaagg agatcaaagt gccagatacg aaggtaatac
    52501 ccactcattt taaatctggc aagattaccg aaaagtattc tgacccattc aaacaagcgc
    52561 aatatgcata ttcaaaaatc aaggaggtta agtgaaatgg caggaaaaca ggtgacggtt
    52621 attccaatga agcctagaca atgggcagca gagaataccg aagagaaacc aaagttgaaa
    52681 gtggcagcct attgtcgtgt ttctactgaa atggaggaac aggcgagttc ttacgaagca
    52741 caggttcaac actatacgga ctacattcaa agaaatcccg attgggagct tgtaggaata
    52801 tttgcggatg aagggatttc aggaactggg acgaaaaagc gtgacggttt taaccggatg
    52861 attgaggcgt gtcaaaacgg cgaagttgag tacatcatca caaaatcaat ttcacgcttt
    52921 gcccgtaata cggttgattg cttgcaatac atacgacagt taaaagaact gaagattgca
    52981 gtgttttttg aaaaggaaaa catcaataca atggatgcga agggtgaggt acttttgacc
    53041 attatggcaa gtctggccca gcaagaaagc cagtcactaa gtcaaaacac caagatgggc
    53101 gtacagtatc gtttccagca gggacagcta cgtatcaacc acaatcattt tcttgggtac
    53161 actaaggacg aagatggtaa cttggtgatt gaaccaaagg aagcagaagt catcaaacgc
    53221 atcttccgcg aataccttga gggcagttcc ttgcaagaaa ttgctaatgg tctaatggct
    53281 gatggcatcc taacgggagg gaagcgaaag ctctggcgag gagaaggagt cagattgatt
    53341 cttcgaaacg agaagtacat gggggatgcg ctgcttcaaa aaacatatac taccgatttc
    53401 ttgacaaaga aacgagtgaa gaatgacggg agttacgctc agcagtatta tgttgaagat
    53461 agccatccgg ccattatccc aaaagatatc ttcatgcaag cccagcaaga actagaccgg
    53521 cgtaaatcaa tgaaaaacaa aaagtcacag tgcttttctg ggaagtatgc gttatctggt
    53581 atcacagttt gcggtgactg tggcaatgct taccgcagag ttcattggaa gaatcgagga
    53641 actgtgtggc gatgcaaatc cagagtggat aaacgagaac ataattgcac gggtcgaaca
    53701 atctacgaaa cagacctgca tggagctgtt atcaaggcaa tcaatgaaac gttaatcgat
    53761 cgtgatgctt ttcttcagca attaaatgac aatatcaact cagttatgac tgatggatta
    53821 actgaccgat tggcagaact agacagtcag ttgaaagagc ttgaagctga aattattagc
    53881 ctggcagttg gcggtcaggg ttacgatgaa ttggcaagcc aaatattttc actaagagat
    53941 gaacgagatg cggttgcaaa acaaatagca gcgaatacta atttgcaaca acgggttgat
    54001 gagatggtag tctttgtaaa agaacacgat gtcatcaatg agtataatga agtgcttgtt
    54061 cgaaggttga ttgagaaggt caccattttt gaaaagaaca ttgttgttga tttcaaaagt
    54121 ggggtgagag tgactgtaga aatttaattt gaatagatag gactagacgt taatgtgggt
    54181 agccatatta acgtctttat tgattggatt tcataaagtg attgatgata taatatatta
    54241 tgtaaaaaat aggctggggg cgaagttctg tgagccaatg gaataccatt gatgaattaa
    54301 gggatatttt tattcgtaaa tatacggatt ctgagttgag taatgaactt gaaaaggcct
    54361 gcagcgaaga atatgaattg atgcgtgatt ataatgggcg tcagatttta gagttattac
    54421 agaatgtaga cgatgcctat ggagatataa aatcagaaaa tgatgctagc aatgacgaag
    54481 tgaaagttca gataacatat aaaaataata tcttggaagt tgggaataca ggcacttcat
    54541 tttcaaaaga tacaattgaa agattgtgct taggaagagc gagtaataaa tcatcacaaa
    54601 atatcggaaa taaggggact ggttttcgtt ccttgctaaa cgatgctgag tgggtcgaac
    54661 tgtattcggg agaatttgct attcgattta gtgaagaatt cacaaaaaag ctttttgagc
    54721 agtatgtatc aagagattct gacaaattca gtgaattaat ttttgagcag aagaaaaatt
    54781 ggaaaaaaga agattatgat ttatgttttc caataatgaa ctgtcctcaa ccaataaaca
    54841 aaattgattc tggttttgat acattaattc gagtaaaact aaaagaaact aatttaagta
    54901 aaggcacgag tgtttctaaa caacttcagc agccatttta taagtcactg ttatttttac
    54961 caaatataac aaaaattgtt attgaaacaa ctgatgatga aaaaatatca gagaaaattg
    55021 tggatggaaa tgatgtattg atagaaaaaa gtgatgttac tggtcaatca ccgtctgaag
    55081 aatattttgt ttttgacaaa gaatctatga taggagataa agttgctaaa attgcactag
    55141 ccgtttctaa agattctgat tacgattact ccaaagaaaa actatattgc tactttccag
    55201 tacgtaattt tggtacacca attcatgcac ttataaatgc accgtttata acaaataatt
    55261 cacgtgatga cgtcccggat gatagtgaac aaataaataa gaaaatattt attgaggtgc
    55321 tcaaatttat ttctgaagta gcagaaaaat tatctaagcc tgaatatgaa gacatagcat
    55381 taaaaatggt aactcctttt aaagagaata aactatggga cttagatgta tttgacttaa
    55441 tgaacgaatt cttgggtatt ttatctaatg cccaaatact accgactgtc aattggcaat
    55501 atatttctat caaagataat ccaaaacttt tccttcaaga attccctgag gagctaatag
    55561 gtgagacatt ttccaccttg ttgcaattat tagaaacaaa cgaaaatgac caactagtac
    55621 aagaacttgc taattataat gaatacactg gcctgtcgtt tgttgtagat gaactatctg
    55681 ataaaatcaa cattctttca tcttcttggg aaaatagaat aaggacaaaa gtgttcttat
    55741 ggtggagtga gcaatataaa agttctccga ttgtccctaa gctattaaag gacaatagtg
    55801 acaattggtt gatgaaatca gataaggtat tcttaccgac cgatacaggt gttactgtgc
    55861 ttccaaaaga gctgtcttct tgggttagtt taagcatttt gaaacaaaca tatgttgatg
    55921 agttaattta tcagattaag acttcatcaa gttggaaaaa taaatgggaa gaaacagcta
    55981 atgcatacaa agctgaacgt acaggagata aacgtattct agatgcattc agtgaaaaat
    56041 atcttgcaat tgaattcact gagcaaagca gttcagattt gatcattgga acgattaatc
    56101 gacaaattga cactgttgaa aaatctatca gtttcattaa ttggttttat gataactaca
    56161 atgaaaaact atcagctgga agtgagctgt ctaaagttcc atttaatctt ccaaacgttg
    56221 ctggtgacgt tcaaccttcc aataaaatgt atattggaaa agagtatggc aatgaattag
    56281 gagataagct ttttcaagat acagattaca cggcattgaa aaaaatagat tgctctagtt
    56341 ttgctgagga gagagatttt ctgtcattta ttcttcgatg tggtgtttcc aaatatccta
    56401 agatttatga taacgattct ttaaaaaatg atgttggatt taaaaagttt atttataaca
    56461 aatataagaa tgaaataaaa tttacgaata ttaattattt aacttctaag tacatcagta
    56521 attttgaaga attaatagac aaattagata cgaaagacat tgtcaattgg ctcaatcaag
    56581 acgaagaatt gacagattta ttatcttcgg taaaaaaaga atcaagtgca agtcagcaat
    56641 cgaactggag tggattgtac tttaattcaa atgagtatgt gaaatacatt ttgaataaaa
    56701 ctgcatggat taagattgat ggagaaaaat atagcccgag acaaattgtg ctatatgaaa
    56761 agctccaggc acacgttccg ggattgtatg gagtttctga acaaagctta atggaactct
    56821 tggggcaacg cattgttgaa cagttggatt tcagaaaaaa tatggcgatg ttttcggatg
    56881 atgaaataag agctatatta gttgagttac caaaattcga taaaggcgaa ataagtcgca
    56941 gattatataa tgaacttata agaaataaaa aaggaatgaa tcctacttat tctaccgaag
    57001 gattatcagt cttagccaaa gatggcttct tttatccaaa caaagaatta aggtatgctg
    57061 ataagcgact gcctaaatca atagaaaaac aaatgcgttt tatttacgtg cctgaaaaag
    57121 ctagtacaac tacgatatat gaatggcttg gggttgaacg cttcaaaaca aatttaaaat
    57181 tggaaacatt tgagttattg gaagattatt ctgaggattt caaaaaagag attgatgata
    57241 tcaaaactgc ggtattatct acgatagatg acaatagtaa gaatgttagt tcattaaaac
    57301 gtattcagat tattccatgc agcaaaatat ctgctaaaga tgttgagcaa gatggtcagt
    57361 caatcgtttt ggaagattat tattttgtgg aaagtaaagg aaaattctac ttaaaattac
    57421 ctgctaattc tattgaaatt aatcaattac gtttgattga tacgttttct tcaacaatcg
    57481 tagacatatt caagcaggtg ttaaatctaa gcttagattt gaatttgatt gaattgttaa
    57541 tttcccgaga tacggaacat aaacaaagaa aaataactga agaattcggt gtagataagt
    57601 ggaatgaatc gtttgagcta ttgtttagcg aaaatgcgtt aaataaaaaa gttaatgact
    57661 ttttgttatc aaatggacta aatgaagagg aatatcctac catttttgaa attgactatt
    57721 catatgcatt aaaagaagaa gagtatcaat tgttggcgat agctctagaa agcatttcaa
    57781 aagatattga ggatttgaat ggattgtcag aattgattca tttggatatt cgtgaatttt
    57841 ggaaactgaa attaagatct tatctagata agaaagcgga acattaccgc cagattttat
    57901 atagcaatgc aaataaaagc ggtgatgaaa tactcaaaga aacattccta gaaaaaattg
    57961 atagatacaa agattatcaa tttgagattt ctgaaattcc aaattcagtt caagtcgaca
    58021 ttgagaaaga aagtaagaag agatttccag agctaagtgt gtcaattact gatgaaattg
    58081 acattgatgg cctgtatgat aataatgtcg aagagttagt cacggttgca gctattacaa
    58141 tagatgattt tgattattat attagaggtc atcaaaaagt ttcttcctta ctgtattttg
    58201 gtattccgtc ccgtttaaga gaagatatac tgatattcgt tactaaagaa aatgatgttg
    58261 atttgaacgg agaaaaatct ggtatctcta ttgaaggaac atcaactgtt caaaccattc
    58321 ttgaaccatc gaatagtgtt agtaggaaag gtaagaacac acgaaatgga gaaagaagta
    58381 aacgagatta tgataataga aactccgcaa atgatgatgc tggtgaaaca gcagaacaaa
    58441 ttgcttacgc tgaattgact cagaatcaaa aacttaatgt aatttggaat acgaaatatt
    58501 ctagtaaatc ttcagatcgt aataagcagc caccaaacgg aattgtttgt gatatgtggg
    58561 ttcatgaccc agagaatggg aatatgtact ttgaggtaaa atcatcaatt acagaatttg
    58621 aaatgtcgat taatgaatat aattctatgt tagataaccc tgactcttac gaggtcgttt
    58681 tagtcaatcg tgataccaaa gagataagtc gtcataaatt tgatgaactt gatggattga
    58741 aacaggcaag tagctatctt ttcaaattta agcaaaagag aagtgacggt tgagtgattt
    58801 gtttccacaa acctattcaa tctatcaact caaaaatcat aaacaaccat tattatagta
    58861 ggaagataca gagtgtactc tcaacgcata tcgaattagt cgcttcattc gttaaaggag
    58921 aataaaaatg gaccaaaaag aaaaaatgaa agaattgatc aaaaagaaac aaggtggagg
    58981 ccgatccaaa caaatggaaa cacctaagaa tgatcgtaaa aacatgagaa aaggccctaa
    59041 aatctttaat aaatagacgt gctaaagaaa gactaagaag tgttaacttt tgttattgtt
    59101 tcttagtctt ttta
//
LOCUS       DANKHJ010000012        52836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000012 DANKHJ010000000
VERSION     DANKHJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="PW113_000840"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="PW113_000840"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2525171.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="PW113_000841"
     CDS             complement(1036..1956)
                     /locus_tag="PW113_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2525172.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="PW113_000842"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="PW113_000842"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2525173.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="PW113_000843"
     CDS             3685..4218
                     /locus_tag="PW113_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2525174.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="PW113_000844"
     CDS             complement(4708..5232)
                     /locus_tag="PW113_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525175.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PW113_000845"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PW113_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2525176.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PW113_000846"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PW113_000846"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2525177.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="PW113_000847"
     CDS             complement(6954..7634)
                     /locus_tag="PW113_000847"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2525178.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="PW113_000848"
     CDS             7760..8629
                     /locus_tag="PW113_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2525179.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="PW113_000849"
     CDS             8655..9170
                     /locus_tag="PW113_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525180.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9810)
                     /locus_tag="PW113_000850"
     CDS             complement(9217..9810)
                     /locus_tag="PW113_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525181.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="PW113_000851"
     CDS             complement(9835..10014)
                     /locus_tag="PW113_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525182.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="PW113_000852"
     CDS             complement(10091..10600)
                     /locus_tag="PW113_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525183.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="PW113_000853"
     CDS             complement(10696..11076)
                     /locus_tag="PW113_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2525184.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /locus_tag="PW113_000854"
     CDS             11406..12155
                     /locus_tag="PW113_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2525185.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12339..13616)
                     /locus_tag="PW113_000855"
     CDS             complement(12339..13616)
                     /locus_tag="PW113_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525186.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13695..14339)
                     /gene="pcp"
                     /locus_tag="PW113_000856"
     CDS             complement(13695..14339)
                     /gene="pcp"
                     /locus_tag="PW113_000856"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2525187.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14538..15482)
                     /locus_tag="PW113_000857"
     CDS             complement(14538..15482)
                     /locus_tag="PW113_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2525188.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15484..16170)
                     /locus_tag="PW113_000858"
     CDS             complement(15484..16170)
                     /locus_tag="PW113_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2525189.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16644..16964
                     /locus_tag="PW113_000859"
     CDS             16644..16964
                     /locus_tag="PW113_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2525190.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17118..18023
                     /locus_tag="PW113_000860"
     CDS             17118..18023
                     /locus_tag="PW113_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2525191.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18020..18793
                     /locus_tag="PW113_000861"
     CDS             18020..18793
                     /locus_tag="PW113_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2525192.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(18974..20398)
                     /locus_tag="PW113_000862"
     CDS             complement(18974..20398)
                     /locus_tag="PW113_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2525193.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20609..21055
                     /gene="nrdI"
                     /locus_tag="PW113_000863"
     CDS             20609..21055
                     /gene="nrdI"
                     /locus_tag="PW113_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2525194.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21120..23102)
                     /locus_tag="PW113_000864"
     CDS             complement(21120..23102)
                     /locus_tag="PW113_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2525195.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23116..24012)
                     /gene="murQ"
                     /locus_tag="PW113_000865"
     CDS             complement(23116..24012)
                     /gene="murQ"
                     /locus_tag="PW113_000865"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2525196.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24015..25076)
                     /locus_tag="PW113_000866"
     CDS             complement(24015..25076)
                     /locus_tag="PW113_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2525197.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25287..25853)
                     /locus_tag="PW113_000867"
     CDS             complement(25287..25853)
                     /locus_tag="PW113_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2525198.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(25881..27383)
                     /gene="eat(A)"
                     /locus_tag="PW113_000868"
     CDS             complement(25881..27383)
                     /gene="eat(A)"
                     /locus_tag="PW113_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2525199.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27641..28762)
                     /locus_tag="PW113_000869"
     CDS             complement(27641..28762)
                     /locus_tag="PW113_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2525200.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            28888..29829
                     /locus_tag="PW113_000870"
     CDS             28888..29829
                     /locus_tag="PW113_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2525201.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(29979..30875)
                     /locus_tag="PW113_000871"
     CDS             complement(29979..30875)
                     /locus_tag="PW113_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2525202.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            31144..32484
                     /locus_tag="PW113_000872"
     CDS             31144..32484
                     /locus_tag="PW113_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2525203.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            32702..33145
                     /locus_tag="PW113_000873"
     CDS             32702..33145
                     /locus_tag="PW113_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2525204.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(33649..35067)
                     /gene="nhaC"
                     /locus_tag="PW113_000874"
     CDS             complement(33649..35067)
                     /gene="nhaC"
                     /locus_tag="PW113_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2525205.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(35470..36693)
                     /locus_tag="PW113_000875"
     CDS             complement(35470..36693)
                     /locus_tag="PW113_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2525206.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(36724..37563)
                     /locus_tag="PW113_000876"
     CDS             complement(36724..37563)
                     /locus_tag="PW113_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2525207.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(37580..38260)
                     /locus_tag="PW113_000877"
     CDS             complement(37580..38260)
                     /locus_tag="PW113_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525208.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(38262..39335)
                     /locus_tag="PW113_000878"
     CDS             complement(38262..39335)
                     /locus_tag="PW113_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2525209.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(39391..39629)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(39672..39905)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(39982..40653)
                     /locus_tag="PW113_000879"
     CDS             complement(39982..40653)
                     /locus_tag="PW113_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2525210.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(40740..43418)
                     /gene="mgtA"
                     /locus_tag="PW113_000880"
     CDS             complement(40740..43418)
                     /gene="mgtA"
                     /locus_tag="PW113_000880"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2525211.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            44077..44514
                     /gene="ndk"
                     /locus_tag="PW113_000881"
     CDS             44077..44514
                     /gene="ndk"
                     /locus_tag="PW113_000881"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2525212.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(44536..45402)
                     /locus_tag="PW113_000882"
     CDS             complement(44536..45402)
                     /locus_tag="PW113_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2525213.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(45562..47655)
                     /locus_tag="PW113_000883"
     CDS             complement(45562..47655)
                     /locus_tag="PW113_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2525214.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(48404..49027)
                     /locus_tag="PW113_000884"
     CDS             complement(48404..49027)
                     /locus_tag="PW113_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525215.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(49186..50583)
                     /locus_tag="PW113_000885"
     CDS             complement(49186..50583)
                     /locus_tag="PW113_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2525216.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(50615..51586)
                     /locus_tag="PW113_000886"
     CDS             complement(50615..51586)
                     /locus_tag="PW113_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525217.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            51960..52385
                     /locus_tag="PW113_000887"
     CDS             51960..52385
                     /locus_tag="PW113_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525218.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        15272 a        10978 c         9272 g        17314 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat ttttttctaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaataac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttttt atagtaattc cttcagatac
    12361 tttcttgcct catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct
    12421 tgtttctgag cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc
    12481 acatttccag attggatatc tttcactact agttgccatt tttcttttga gactgtacgg
    12541 tctttcttat cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca
    12601 tttctgacta tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat
    12661 tgcttccaaa taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat
    12721 tccatctgag cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata
    12781 aatgtttcaa aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa
    12841 tctttacgta aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct
    12901 tctgcaaagt cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg
    12961 tgttcactta ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc
    13021 atttctactt ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc
    13081 tgtttcatcc gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga
    13141 aaatcgatat tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc
    13201 attccctgat ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg
    13261 ttgatgccta gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt
    13321 aattctctac cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga
    13381 ttgatcttga agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa
    13441 cgagagctga ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat
    13501 tttgcataga tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag
    13561 cttttgctga tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct
    13621 gtttctttcc tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag
    13681 tgtttcttgc taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct
    13741 actgctgctt caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc
    13801 gtacccactg gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct
    13861 cgaattgccg ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag
    13921 acaaatgttc ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt
    13981 gtcattgctt tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc
    14041 gtatcgaatg gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg
    14101 acacgttcga acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct
    14161 ggttggtgct gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt
    14221 acttccactt tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca
    14281 taggctggat tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc
    14341 tgttgttcct ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt
    14401 gaacagcgta aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat
    14461 tcttataaaa aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc
    14521 acaacccctc tcttctatta tttaaataga atcatataag caatttggaa aactagcata
    14581 aataacgcaa taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc
    14641 gctacaggca cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg
    14701 caagtcaatg caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt
    14761 gcaccgatcc caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta
    14821 aatatcacca tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg
    14881 atcgtaccta ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct
    14941 agagaagcta aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct
    15001 gcatcatcta aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg
    15061 gctgcaaaaa caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac
    15121 aagatcgcaa agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct
    15181 gacatttttt ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta
    15241 gcaattgctg gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat
    15301 cttcctcctg caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga
    15361 ggattggttt tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg
    15421 atataaagga tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc
    15481 atcttattgc ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa
    15541 gtagacactt gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg
    15601 tcccaatgtt acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc
    15661 tcctccgatg aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat
    15721 accttttagt tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc
    15781 caatgccatc ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt
    15841 aaaagccgca aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac
    15901 tttcttgaat cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt
    15961 tcctgttgcc aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt
    16021 acctaaaagt tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa
    16081 agcaaccatt acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa
    16141 gacgcctaaa aggctaagat aattttccat atattttcct ccataaaatg acaatatttt
    16201 tttaattata aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat
    16261 ttttaaatat ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa
    16321 gcttttttta atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt
    16381 aaatgattaa taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc
    16441 gctaacgata tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata
    16501 tctggatagg tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa
    16561 gaggatgatc attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt
    16621 tatagttaag gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg
    16681 aagtattctg gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt
    16741 tttacacagt gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt
    16801 tgccgatgag tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa
    16861 ttggtgtggt cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc
    16921 tactcatcag tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta
    16981 ctgatcagaa aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa
    17041 ataagtccaa tgacctagcg acttctccac tcatcaggtg tatggttgta ctatctatag
    17101 aaaaggagtg aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc
    17161 acaaacaagc cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag
    17221 taggtcctaa tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt
    17281 actcagatgg aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt
    17341 tggaacaagt aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg
    17401 atcatctgaa gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc
    17461 agctcaagat ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac
    17521 aaaaattggg aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc
    17581 cgatgaacgg tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg
    17641 ctactcaggg cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg
    17701 tcgatgagct gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat
    17761 tgaaccaaac agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa
    17821 cgatcttgac agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag
    17881 aaaacgaaga attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga
    17941 cagacgtcca gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag
    18001 taaaggggga agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa
    18061 gaaaaagtca actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact
    18121 tctagccaaa aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag
    18181 tgccacatct tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga
    18241 agcacaatat ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt
    18301 agtaagcaaa tggatcacat tagttattta ttctgtttat ttataccttt tagctattat
    18361 tgtcactgtg ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc
    18421 tactggtcaa acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt
    18481 atggctgatt ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc
    18541 catctcagca ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc
    18601 actcatccaa aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa
    18661 ccaaattgga aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct
    18721 attcggtaat ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg
    18781 aaaaaatgta tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc
    18841 cgattggctg tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt
    18901 cacagctcct ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac
    18961 ttcttttata cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc
    19021 cgaccatcgt cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt
    19081 tttgctcctt acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa
    19141 gcattacttc attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa
    19201 gccctactaa aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc
    19261 ctaagaaaag ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag
    19321 cactgtcagt gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga
    19381 tagcataagt tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt
    19441 cttcagccat gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa
    19501 ctaaagaaat gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt
    19561 caaacacata gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg
    19621 tcccatatgg aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa
    19681 tcgttgcttt cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt
    19741 caataccaac aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg
    19801 ggatcggctg tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt
    19861 ggatcgaaaa cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga
    19921 atgtgacgat agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat
    19981 atccccataa aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata
    20041 ctgggaagaa atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc
    20101 agcatgtgag ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt
    20161 ctgcgtaact aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg
    20221 acaaggagag catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc
    20281 cagccgatcc ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa
    20341 ttactaaggc agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt
    20401 aagcatcctc caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt
    20461 acttaattaa taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca
    20521 agcaaagcgc ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata
    20581 ctaaaaggaa agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa
    20641 tacgcgtgcc tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc
    20701 tgtcaatcct gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac
    20761 agaaccattt tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg
    20821 tgatacagaa atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa
    20881 actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac
    20941 agccaaacaa tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg
    21001 aacaccatct gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa
    21061 aagaagaagc tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat
    21121 taagctttga catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca
    21181 aagtttttta cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct
    21241 gctttgatcg cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct
    21301 tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga
    21361 agcaagattt ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca
    21421 gagctgacta ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag
    21481 ccttcaccca tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact
    21541 gttccgtcag caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact
    21601 ttttcaggca cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg
    21661 tctttaggaa taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat
    21721 ccaaggatgt agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa
    21781 ggtccaatag cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct
    21841 ccaccgatac aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat
    21901 ggttcgccga ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct
    21961 ttatcttttt tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc
    22021 atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt
    22081 ggagtcaaaa tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg
    22141 aagccggcaa atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca
    22201 gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat
    22261 gaaagagttg gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc
    22321 tcaacgattg acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct
    22381 gcaagagctt caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc
    22441 atcacaacgg ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca
    22501 ttgatacctg tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca
    22561 tcgatatatt gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa
    22621 actgcggcaa tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag
    22681 atattagaaa tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt
    22741 gccgctttca tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt
    22801 gctgtagctg tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc
    22861 atttgctgtg ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct
    22921 tctactacac caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca
    22981 ttgatgatct tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg
    23041 ccgcctgccc ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc
    23101 atggtctttt ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc
    23161 ttgagcttcc tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg
    23221 atctgcttct tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat
    23281 gattcgtttt gagcgttcga ttagtttttc atttgttggt ttgacatcga ccatcaagtt
    23341 attgaatact ttacctatac caatcatact gatagtagaa atcatattca agataagttt
    23401 ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac
    23461 ttcgattggg aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc
    23521 tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc
    23581 gctagctgcg ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc
    23641 ttgccctagc tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc
    23701 accagcaatt agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc
    23761 agcatctaac acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc
    23821 ttttttaaag gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc
    23881 aaccgcagcg gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact
    23941 catctcatct aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt
    24001 gattttgctc atttttatct tccttctttt cttataaatt gatagttcat accagctttg
    24061 atttggttga tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca
    24121 tccgctggca aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg
    24181 ttatctaaag taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga
    24241 atctctcgaa aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc
    24301 gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag
    24361 actgtctgtt ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca
    24421 tctccgatgt aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga
    24481 tggatccctc gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct
    24541 gtatctcctg gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt
    24601 ttctgatacc attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct
    24661 tcaatctgtg agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc
    24721 gtgctagcat tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa
    24781 atatgataat ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct
    24841 aatgaaaaac ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta
    24901 ttctttttcg cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct
    24961 tctggtatat gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata
    25021 tactgtttct tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta
    25081 tccctctttt tctgtatccg tcttcatttc tatgactaga gtatatatct aaagaaacaa
    25141 aatttcaata cttttattaa aataattaaa tttcatttct aaaggttgag tttatattat
    25201 caaaagaatt gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat
    25261 tttatttaaa taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc
    25321 atgaatgtaa agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc
    25381 aaggcattat ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat
    25441 aaaggaacga tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg
    25501 aacgctaaac gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca
    25561 aaatccattt ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact
    25621 gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca
    25681 ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc
    25741 ggcacatctg cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc
    25801 gttacataaa gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt
    25861 tgttcttgag attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta
    25921 tccaggaagg tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt
    25981 tgttctaatt gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat
    26041 agttcagctg gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc
    26101 atctgctcga tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc
    26161 aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg
    26221 tcttcataat tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct
    26281 ataaccttgc cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg
    26341 ttaggacctg aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat
    26401 aacagatttt cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac
    26461 gcttggctat tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc
    26521 tcgctgatct gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca
    26581 gcatcagcac ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact
    26641 tgtttctttg ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt
    26701 ttctttagcc tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca
    26761 cgaagttttt tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt
    26821 ttttcgattg ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc
    26881 acgatgaagc cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata
    26941 tccaaatgat tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt
    27001 gccagtagga cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt
    27061 tcaggatctg tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta
    27121 atatcattta acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag
    27181 gcaaattctt gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc
    27241 agtaaagttg tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca
    27301 aaatttagat ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga
    27361 ttttttattt cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt
    27421 cagaactggc taaagaggat gcaagggttg gatacacaca aaaagaccca tacatcgaaa
    27481 aaaccaagcc aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg
    27541 aatgcagctt tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat
    27601 atttgaaatt acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga
    27661 aaaatctgct aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca
    27721 ttgcttcttc taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag
    27781 aaagtccttc tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt
    27841 tttttgcaag cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc
    27901 gcccttctcc tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt
    27961 ggctgactaa atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa
    28021 taccgcctgc tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta
    28081 tttgctgagc aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc
    28141 ctttttgtct tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat
    28201 ctgcagcgat aatcaatttt actttagaaa caagaacgtt tcttcgatca aaatgaagtt
    28261 ttttagtaga taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt
    28321 ggagtaatcc tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt
    28381 ctacttcttg aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc
    28441 ggatagtttg atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga
    28501 tgactgcggt gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga
    28561 ataagagatc catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt
    28621 ctgcagtatt ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt
    28681 cccagccatt tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat
    28741 caattgccgc caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt
    28801 ttcatttttt tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga
    28861 actcattgaa taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg
    28921 ggcagccgaa tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt
    28981 tggcctgctc acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa
    29041 gaagtggtcg caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac
    29101 gtggacttga tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc
    29161 caatccatta ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa
    29221 gatgtactgg aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca
    29281 gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag
    29341 aacttggaac cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca
    29401 aatttaaatc cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta
    29461 aatggaacat tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg
    29521 caaaaagcgg cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca
    29581 aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat
    29641 cctgacaaca tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt
    29701 ttgactgaaa tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt
    29761 gctacaccat attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc
    29821 gcagaataaa aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta
    29881 agcccaataa aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg
    29941 tttgtttcag tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag
    30001 aggtcatatt cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa
    30061 ttttctgcat cataaaattc caatagctct gataggattg gcttaaatgg tgtatgcaga
    30121 aattccggta attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa
    30181 aaatgataat gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat
    30241 tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc
    30301 gattcccctg tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct
    30361 cgatgtggaa gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag
    30421 ctttctgctc gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt
    30481 tcgctagaag aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt
    30541 tcttttgctt ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc
    30601 tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt
    30661 gcattggtaa atgggcgcac gcgtcgagcc atctccatat caaatccata atctaaaaaa
    30721 acgatcagct gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt
    30781 aaatcttttc cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta
    30841 ttttttgcaa ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca
    30901 ctttttccta tgatagcctg tttaaatagt aaaaagccag taaaacgttt cctatatttg
    30961 tatgaaaatt taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt
    31021 cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt
    31081 taaatattgc tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca
    31141 tttatggaag ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct
    31201 ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc
    31261 ggcccttctg tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa
    31321 ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata
    31381 cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg
    31441 attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg
    31501 cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt
    31561 tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc
    31621 attatcttac tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc
    31681 gttttcccta cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag
    31741 ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct
    31801 atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc
    31861 atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct
    31921 tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat
    31981 atcccatttg cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc
    32041 aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt
    32101 tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt
    32161 tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt
    32221 ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg
    32281 gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt
    32341 gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta
    32401 actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat
    32461 gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata
    32521 gatggagcta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa
    32581 taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg
    32641 attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta
    32701 aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg
    32761 tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt
    32821 catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat
    32881 gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg
    32941 ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat
    33001 gattcctcat cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga
    33061 agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa
    33121 aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc
    33181 agaagttatc agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg
    33241 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct
    33301 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag
    33361 aggttgtaaa atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct
    33421 catattttgc cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga
    33481 gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc
    33541 cacatttttg tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc
    33601 gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa
    33661 ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc
    33721 tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa
    33781 gtaagtgagt gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg
    33841 cactaagtag ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc
    33901 aaatcctgct ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa
    33961 ttgttcacca ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt
    34021 tggtgtgttc gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc
    34081 gatcaacaat ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact
    34141 ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa
    34201 ccctgattcg atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat
    34261 cattcctact gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca
    34321 tgcacacagt aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc
    34381 ttctagaaca tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc
    34441 taaaatcatg aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg
    34501 cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga
    34561 taatggagac atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc
    34621 tggatttaag cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac
    34681 agtaaaggca ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac
    34741 aaaccactgg acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc
    34801 ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc
    34861 tggtttgatg ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac
    34921 tgcatacaac atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa
    34981 tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa
    35041 aaaacttggt gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt
    35101 ctaaaaaagt taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata
    35161 aaaataaaaa gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa
    35221 aaacagatct gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt
    35281 cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc
    35341 gctactgcgt gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg
    35401 aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttca cacacaagtt
    35461 tattttttac tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc
    35521 ttgcttgatt ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag
    35581 tagttctgct gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa
    35641 ttcttctgtg tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg
    35701 tatgacttgg ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc
    35761 tatttcttca tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga
    35821 aggagtaacg atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc
    35881 cgtacttatc gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt
    35941 ttgccgattt cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc
    36001 agctactcct ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg
    36061 tagagcgttg attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc
    36121 aggtgctgct gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc
    36181 taaagattct gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac
    36241 atcctcaaaa aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc
    36301 ttcccctggg gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac
    36361 tatgctggcg gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat
    36421 acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat
    36481 ataacgtgca tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc
    36541 tttttctaat tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg
    36601 atgttcgtgg atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc
    36661 cttcaattgg tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt
    36721 ttcttatttt ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc
    36781 gattactttg actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt
    36841 gttttcatct tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac
    36901 tggttctaag aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct
    36961 attgatcact gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc
    37021 agaaatgtct agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt
    37081 tgccttatcc actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt
    37141 tgtaacatca ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa
    37201 agtgttagaa taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt
    37261 tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat
    37321 cgatccatct gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa
    37381 attaatgttc tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc
    37441 aataatccct agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct
    37501 acaccctgcc aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt
    37561 cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt
    37621 gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga
    37681 atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag
    37741 ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag
    37801 gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt
    37861 ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa
    37921 aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc
    37981 caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac
    38041 gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct agtaatccat
    38101 tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg
    38161 ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca
    38221 aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc
    38281 actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg
    38341 atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa
    38401 ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt cgctgttact
    38461 tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt
    38521 aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt
    38581 tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca
    38641 ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc
    38701 gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt
    38761 aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga
    38821 tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt
    38881 gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt
    38941 tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat
    39001 tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga
    39061 ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag
    39121 agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa
    39181 ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca
    39241 tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt
    39301 ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa
    39361 gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc
    39421 tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga
    39481 tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat
    39541 tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc
    39601 tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata
    39661 aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc
    39721 ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg
    39781 aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac
    39841 tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa
    39901 atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa
    39961 ttcaaattat tttttctata tttatttttc tgttttttta tttaacattt caataacagt
    40021 tcgaagatct tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt
    40081 tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt
    40141 tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc
    40201 caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt
    40261 aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt
    40321 aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc
    40381 ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc
    40441 acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc
    40501 aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat
    40561 atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa
    40621 caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat
    40681 acacatagtt tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt
    40741 tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga
    40801 taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct
    40861 aattcagcac caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg
    40921 ataacaaaca tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt
    40981 gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat
    41041 gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca
    41101 ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga
    41161 ccaaagtaaa ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa
    41221 tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc
    41281 aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc
    41341 acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc
    41401 ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa
    41461 aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata
    41521 tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga
    41581 ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat
    41641 acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca
    41701 ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat
    41761 tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct
    41821 tgtttcgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct
    41881 gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca
    41941 atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt
    42001 tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt
    42061 tcttcaatcg cgccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca
    42121 ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta
    42181 tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc
    42241 cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct
    42301 tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc
    42361 gtacataaca catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt
    42421 ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc
    42481 atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc
    42541 ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc
    42601 agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat
    42661 agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc
    42721 gcacttccac tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt
    42781 tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat
    42841 gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata
    42901 tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg
    42961 tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc
    43021 gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg
    43081 aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg
    43141 atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc
    43201 aatatttcta caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt
    43261 tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca
    43321 aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt
    43381 ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct
    43441 ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat
    43501 agccctggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat
    43561 ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc
    43621 caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct
    43681 ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc
    43741 atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat
    43801 gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc
    43861 agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta
    43921 atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta
    43981 aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg
    44041 ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc
    44101 aagccagacg gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag
    44161 ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat
    44221 tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt
    44281 cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt
    44341 ggagcaacaa aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg
    44401 gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc
    44461 cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa
    44521 ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga
    44581 cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt
    44641 cgtcttttct tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt
    44701 ttcatagatc actgtacttc tttctttatg tgataagctc cgtttcatat cttcattcac
    44761 actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa
    44821 agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat
    44881 tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat
    44941 agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca
    45001 ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag
    45061 attcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat
    45121 ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt
    45181 taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt
    45241 cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc
    45301 aatcccgact agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt
    45361 cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa
    45421 tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa
    45481 taaaaaaatg tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact
    45541 actgaaggtt ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt
    45601 tactggatat aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg
    45661 ttcgcctttt tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt
    45721 tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt
    45781 cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc
    45841 atcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc
    45901 tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc
    45961 atgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa
    46021 tagatgtagt aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc
    46081 tttagtataa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa
    46141 ttcatcccat gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt
    46201 cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt
    46261 tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata
    46321 attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt
    46381 ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg
    46441 ctcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt
    46501 ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata
    46561 tacatgatct ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc
    46621 ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt
    46681 gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact
    46741 tgtctttccc tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg
    46801 gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa
    46861 ctgctggaaa ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt
    46921 ttcgggattt ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt
    46981 actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt
    47041 atacgcatcg attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa
    47101 ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca
    47161 ggtaacagcg atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac
    47221 cttcaaccaa accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg
    47281 gggcttcatc aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa
    47341 tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa
    47401 caaaagcgta tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt
    47461 aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat
    47521 aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt
    47581 cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt
    47641 tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg
    47701 aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat
    47761 tattccgtcc acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag
    47821 agataaaagt cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta
    47881 ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca
    47941 ggacagcttt ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca
    48001 agtcacaata tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga
    48061 gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat
    48121 tagattatgt caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca
    48181 tctgcggtaa taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc
    48241 ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct
    48301 gaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc
    48361 tctaatactc aggtcaaaac gctgatttca caacctctgt ttttcattgt ccataggatt
    48421 gatattgtcc ttgtgatgac tcattcgtaa taattgaggc ttgattttct tcacttcccg
    48481 ttatcgtttg tccactgcca gaatctgaag gacttgttcc tgtcgactcg tttttttgag
    48541 aagagacagt tgttttcttg acttgttcat ttgtcttttt ccattccgct aaacgatcgg
    48601 cgaccacttt tttgtctact ttttcagaat aagcggtgat cacttttcca tttttcaaac
    48661 gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg tctttaaatt
    48721 gatattgtct gagagaaatc gcactaagta cgtcatcgat attaataaat tgattttctt
    48781 cttttccttt tggcaaataa atgtccaatt cgcctttttc gtaattggca gttattttat
    48841 actccgccca ccaataagtt gagaattctt ttttgaaatt atctactgat tctttgcttg
    48901 ctacgatttt ttctgtttct ttctgagcaa gatctgctgt catttttcct gaaaatgctg
    48961 cggataaaat aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt
    49021 tcctcatgct ttcctccact cctatttaat tttgactatt ctattatata caaaaaatga
    49081 aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt ttcacaaaat
    49141 ggtaagaatg gagaatttca cgcatttgct ctcaactatt ctttcttaag cctcaaatcc
    49201 gtttttatct gatacgtctt tgaaccaatg acccgatttt ttgattgttc tttttccatc
    49261 ttgtgctaag tctactgaaa taaagccata tcgattctta tatgcattgg tccacgacca
    49321 gttatccata catgtccaca tatgatagcc taaacaattg gcaccttctt gaatggcttg
    49381 atggacatat ttcagatgag attggacaaa ttcgatccga taatcatcgt ggatcatgcc
    49441 atcttctttg ataaatcgtg cttcgttttc tacacccata ccgttttcag aaataaagca
    49501 gtcgatattt ccataatgat ctcgtacatt gatcaaaata tcatagattc cttgttcata
    49561 gatctcccat ccgcgataag gattcatttt acgaccaggc atttcataat tgtcgaaata
    49621 atcctcaggc attggaccgt ttttagtatc gattggtgtt tcttttgctt ttacccggcg
    49681 tggctggtaa taattgatac ctaaaagatc tactgtattt tctttgataa ccgtcaaatc
    49741 ctctgcatgg atcacaggca tgagatcaag ttcttttact atctcaatca attcacttgg
    49801 aaactcgcct tttacagctg gatcaaggaa tgaacgattg aagaacgcat ctgctatttg
    49861 cgctgcttta acatcttcag ggtttgattc gtctcttgga tagcttggtg tcagattcaa
    49921 gatgatacca ataacaccac cttgctccat ttcatggtaa gatttgatgg ctaacgctga
    49981 agccaacgct tcatgatagc caacttggat cgcttttttc atatctgact cttctggata
    50041 atgccatcga tataaataac ctgcttcgac tggaacgatt ggttcattat gagtaaacca
    50101 tttttttact ttcgaaccaa acaattcaaa acaacttttg gcatattttt cataggcttc
    50161 aaccgtttct cggttcaacc atccgccttt ttcttgcaga tacatcggca tatcgaaatg
    50221 atacagattc acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat aacgatgata
    50281 gaaacgaacg gcttcttcgt tgattgttcc agattcatca ggtatcaaac ggctccattg
    50341 gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc aactgtacat cctcttgata
    50401 cttcgtataa acttgcgagg ttttttcagg tcctacttga tcaaagaact tttgcggttc
    50461 ttttttgtac caataatcaa aaatgttctc tccttttcca tcgcctgaaa tccttccttc
    50521 tgtttgtggg ccacttgctg cagaacccca ccaaaaattt gctggaaatt gatatttttt
    50581 catcaaagca cctctttttc ttttttagtt cgttttagtt gattccatgt atgatatttt
    50641 ccatcacaat cactttttca ttttcttcct cttctttttt gattcgtttt aacagctgtt
    50701 ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat cgtgattttc ggaaggatac
    50761 tcagatcgtt tttatctaaa taatcatagt gactgaacgc aatatccttc ccgattctga
    50821 caccttcttg attgaaacat tcgatggcac caatcgacat attgtaattg ctgacaaaaa
    50881 tagcagttgc ccctttttct aataatgctt ttgccccgta atagccgctt tttctagaat
    50941 agtctcctgt aaagacctct aattggatcg atggatcatt ttggaccgct tctttggctc
    51001 ctgctaaacg ttctcttgct acgtagtctg tttgagtggc agcaataaat ccgatttttt
    51061 tatgtccttg ttcaccatga tgagcgatca cttttgctac actttctcga tcattcacta
    51121 atattgtatc aatacctgct ggtccttctg gacaattcaa tgcgataact ggaatactca
    51181 tttttgtgag catttcaaat ccagaccact cttcttcgga taagaagaca atcagcccat
    51241 cgatcacatg cttcatcagg tattcaattt tttgttcaat tttttctggc tgatcggcaa
    51301 acccgcttaa taataagcta tacccatttt cttcagctac tctttcgatc cctgagacaa
    51361 cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag tccaatagag aagcttcgat
    51421 tattcttcaa ccctttagca atgatgtttt ctttatagtc caacgctgaa atcgcttctg
    51481 ctattttttt catgttcttt tccttcagtt gctgaccatt caagtaacgg gaaactgttc
    51541 ccactgaaac accagccatc ttagccacat cacggatcga tgccatacat tctcactcct
    51601 gccagttaca tttgatttct ctttcttgtt gttcgattat gatgaccgca ttctttgcat
    51661 agtgttgctt gtcatcaata tccatataac taaagtcttc agtagagcaa cttcctctca
    51721 ctattaatta tacttaaaca agtatttgaa ccggttcatt tttaatatag cattttttta
    51781 aaagattgca acactttttt aaaacgcttt ctgaaagtgg cggatttttt gcctttttaa
    51841 aattttattc taaaatttac tctatttgtg ggtgttttgt gcgtttttct cttccatccg
    51901 tttgaagttt tacattccaa tggtataatt tctttatcct atcaaatggg ggaagtattt
    51961 tgaagaaatt aatcgtacta tttattttgt ctgcgggact tttgacattg actggctgtg
    52021 atttgtttgg cggctctgac caatcctcgg acgaaacaac cgattcttct gtcttaaaag
    52081 cggaaaaagc agataccacc aacagtgtat atacgctaaa agtacgtaaa gatatggatt
    52141 tatccgcggg acagttgaca tttacagata aaaaaatgga atggacacgc acgtatcaga
    52201 cacaaggaac tgattcttct gaatcatctg aagaaaaaac agtgctgaca gatatcaaga
    52261 tcactaccaa agatgacgaa tatattattt caggaaaaga aaataaaaaa gaaacaacag
    52321 tcatatttac aaaaatcggt aattaccgtt tgaaggatga ggatggcaac atctacagtc
    52381 tatagtgccg tctgttggat cttcttttgt ttctcccctt cttttctgct atagtaaaga
    52441 agcaagatga ttgtaaagga gacgaaatgg atgatcgata gtcctgatta ttgtacatgt
    52501 aataacaaca aaaatatcca tacagaattt aatgaccaag gaagcttgag catatgcgac
    52561 aactgtcaca aactagtcaa agattcattt aaaacatttg aatcttatcc tgttcaaaat
    52621 tatgtaaaaa gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg ctgattttta
    52681 tgaacttcaa actcagcttc ttcctttgat caagcttcat cagtaaaaca aacagtggat
    52741 catttcagtt tacaaatcaa agagtaaaga tttatttatt tcaaatattt gaatataaaa
    52801 taaccaaacg atagaaaggc ttattctaca tcacgt
//
LOCUS       DANKHJ010000013        47374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000013 DANKHJ010000000
VERSION     DANKHJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(29..1261)
                     /locus_tag="PW113_000888"
     CDS             complement(29..1261)
                     /locus_tag="PW113_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2525219.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="PW113_000889"
     CDS             1516..2085
                     /locus_tag="PW113_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2525220.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="PW113_000890"
     CDS             complement(2263..2703)
                     /locus_tag="PW113_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2525221.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="PW113_000891"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="PW113_000891"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2525222.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="PW113_000892"
     CDS             3657..4580
                     /locus_tag="PW113_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2525223.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="PW113_000893"
     CDS             complement(4805..5044)
                     /locus_tag="PW113_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525224.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="PW113_000894"
     CDS             complement(5049..5468)
                     /locus_tag="PW113_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2525225.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="PW113_000895"
     CDS             complement(5695..6933)
                     /locus_tag="PW113_000895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2525226.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="PW113_000896"
     CDS             complement(7040..7675)
                     /locus_tag="PW113_000896"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2525227.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="PW113_000897"
     CDS             complement(8017..8652)
                     /locus_tag="PW113_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525228.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="PW113_000898"
     CDS             9164..10306
                     /locus_tag="PW113_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2525229.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="PW113_000899"
     CDS             10299..10505
                     /locus_tag="PW113_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2525230.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="PW113_000900"
     CDS             10584..11777
                     /locus_tag="PW113_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2525231.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="PW113_000901"
     CDS             11982..13121
                     /locus_tag="PW113_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2525232.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="PW113_000902"
     CDS             13114..13914
                     /locus_tag="PW113_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2525233.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="PW113_000903"
     CDS             13914..14741
                     /locus_tag="PW113_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2525234.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="PW113_000904"
     CDS             14719..15453
                     /locus_tag="PW113_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2525235.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="PW113_000905"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="PW113_000905"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2525236.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="PW113_000906"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="PW113_000906"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2525237.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="PW113_000907"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="PW113_000907"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2525238.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="PW113_000908"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="PW113_000908"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2525239.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="PW113_000909"
     CDS             19038..21071
                     /locus_tag="PW113_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525240.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="PW113_000910"
     CDS             21115..22395
                     /locus_tag="PW113_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2525241.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="PW113_000911"
     CDS             22605..23411
                     /locus_tag="PW113_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525242.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="PW113_000912"
     CDS             23423..24643
                     /locus_tag="PW113_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525243.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="PW113_000913"
     CDS             24633..26219
                     /locus_tag="PW113_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2525244.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="PW113_000914"
     CDS             26258..28396
                     /locus_tag="PW113_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2525245.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="PW113_000915"
     CDS             complement(28764..29747)
                     /locus_tag="PW113_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL2525246.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="PW113_000916"
     CDS             30125..31516
                     /locus_tag="PW113_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2525247.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="PW113_000917"
     CDS             31532..32572
                     /locus_tag="PW113_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2525248.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="PW113_000918"
     CDS             32591..33676
                     /locus_tag="PW113_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2525249.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..34671
                     /locus_tag="PW113_000919"
     CDS             33709..34671
                     /locus_tag="PW113_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2525250.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTPKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            34664..36091
                     /locus_tag="PW113_000920"
     CDS             34664..36091
                     /locus_tag="PW113_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2525251.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            36093..37163
                     /locus_tag="PW113_000921"
     CDS             36093..37163
                     /locus_tag="PW113_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525252.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            37150..38571
                     /locus_tag="PW113_000922"
     CDS             37150..38571
                     /locus_tag="PW113_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2525253.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            38584..39591
                     /locus_tag="PW113_000923"
     CDS             38584..39591
                     /locus_tag="PW113_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2525254.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            39603..40607
                     /gene="neuB"
                     /locus_tag="PW113_000924"
     CDS             39603..40607
                     /gene="neuB"
                     /locus_tag="PW113_000924"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL2525255.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            40604..41752
                     /gene="neuC"
                     /locus_tag="PW113_000925"
     CDS             40604..41752
                     /gene="neuC"
                     /locus_tag="PW113_000925"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL2525256.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            41725..42402
                     /locus_tag="PW113_000926"
     CDS             41725..42402
                     /locus_tag="PW113_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2525257.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            42392..43534
                     /locus_tag="PW113_000927"
     CDS             42392..43534
                     /locus_tag="PW113_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2525258.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            43547..44524
                     /locus_tag="PW113_000928"
     CDS             43547..44524
                     /locus_tag="PW113_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2525259.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            44524..45432
                     /locus_tag="PW113_000929"
     CDS             44524..45432
                     /locus_tag="PW113_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL2525260.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            45433..46185
                     /locus_tag="PW113_000930"
     CDS             45433..46185
                     /locus_tag="PW113_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2525261.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            46190..47137
                     /locus_tag="PW113_000931"
     CDS             46190..47137
                     /locus_tag="PW113_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2525262.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        15836 a         7486 c         9104 g        14948 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa agataatgag
    34021 tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg aatagatcta
    34081 gatcaaatta aacataaaga attttattat aaacatatag atgtatatca aaaacatgat
    34141 tatcctagaa ttaataggaa taacacaact gcatttaaaa tttttagagc tataattaat
    34201 cgttgtctga ggatgattaa acttccccca aaaattagac accataaatt tgatttgtat
    34261 catggttctc aatggtgggc tttgtcgaaa gaatgcctaa cagaattaat acaaatgtat
    34321 gaacaaaatc aacaagacta cctgaatttt aaaataggta tgtttgcccc agatgaaaaa
    34381 ttttttcata cacttttttt taattcctcg ttcaaaaata aaaatgttat tggaggacca
    34441 gatttaccac ttgaattgaa aaatattgaa gaaactactt tacaaactcc gaaattagca
    34501 aatattcata ttattgatcc ttccatgaac aaatggtttg atgaaaggga tatagatcaa
    34561 ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat cggattactc tgataaacta
    34621 ttaaatagaa ttgataaaga aattttgaaa gttggaaata tagatgaata gaatagtacc
    34681 tgttttagag aagagtttat taagtcgaga tgcgtttcga gtaataaatt taatcatttg
    34741 tctgctttat tttatgccta gtacttttaa tgtaactaat atcccgatga aaatagcttt
    34801 tgcttggggt ggaattatag ttttgtatga ggtttttata aagaaaaatt tttttaaaat
    34861 gcgtttttct tggctactga ttggagcaat gattagtttt atcttaacta taatcattaa
    34921 cagagaaaat tttttaattc catctattta taattttgga tatttgctga ttactttgat
    34981 agtaattttt cctgcagact tttcacaaag tgaagaggaa aaaaagaaaa aaatggtgta
    35041 ttttaataat gttttcatta tcataatttt tttagctgcg ctaatatcta tctatcaatt
    35101 tatttttttg atttcttatc atgtcccaac aggaacgcca ggattacttg caagacaagg
    35161 ttttattgaa catcggttat ttggtgtcta tacaagtcca aatgttggag ctatgtttgg
    35221 atatacttct attattttaa gtttaattag tgtattaatt tcaaaaataa caaaaacgat
    35281 tcgtgttttt tatatgatga attttatcgt acaaatattt tatattacac tttcttcgtc
    35341 tagaggtgcc caggtagtaa tttgttcttt tttagtatta tttcttttgt tgtttgccaa
    35401 ttcaaaattt agaaagagca ttagagaagt actacctgta aaaatcaaat ggatggtaag
    35461 catattatta gttctattgg tatatttttc ttttggtact aattatgcta aggatttttt
    35521 agcaacaatt cctgtatcga ttgatagaaa agtagaagtt atagagtcaa atgaatcaga
    35581 taaaactcca cttccagaaa aagaagtaat attacagcat tctagtgata actccgaaat
    35641 ttcagctggt cgttttacga tttggaaagc tgcgcttaaa gttataagac caactttgtt
    35701 atttggttat ggagagaccg atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt
    35761 agatcttgat actacagatg tgaatgaact aaaaagagca catggaaaca tgcataatgg
    35821 atttcttcaa gtacttgtat caagtggtat aattgctttt atatgtatct atgctttcta
    35881 tgtgttaaat atctttagtt tactcaaaac attttttaga agtaaagtga attatgcgtt
    35941 tcttggtatt gttttaattt ttatactatc tatttttata gatgaaatgg tggaggctca
    36001 tgtacttttc aataagcgcg atgtcatttc aattgttttc tggtattatt tgggaagcat
    36061 ttcttacttg tatttaaaaa aggagaatta atatgaagac agctttcatt gcttggactc
    36121 ctttacattt gattaatata ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag
    36181 atttatttat ttattctgaa tttgaaaacg ctagaagata tagtgagcgt ataaaagaat
    36241 tagggctatt taatgatgtt gtttttgtga atcaccggaa aatcggttct tcctttaaac
    36301 gtaaaattaa cttgttaact aaccataatc aatttttctc ggatgattta tccgtttatg
    36361 atgaaatttt tatacagggg gaaaattatt tcacaaaaat acttttctcc aatgtttaca
    36421 aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg tttgggtact tatttgaaga
    36481 agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta aataaaaata
    36541 gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg ttactttatg
    36601 atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca
    36661 tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc
    36721 aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata
    36781 gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc
    36841 gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa
    36901 atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta
    36961 tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata
    37021 cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt
    37081 ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt
    37141 tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc
    37201 tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt
    37261 gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca
    37321 attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac
    37381 tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta
    37441 attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg
    37501 aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat
    37561 atccagtctt attttacgat taactttaac cattttaaat atttgatttt atcttttata
    37621 tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat
    37681 aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt
    37741 tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct
    37801 ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc
    37861 gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt
    37921 agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca
    37981 tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat
    38041 tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca
    38101 aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta
    38161 ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa
    38221 acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg
    38281 attttcatac ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt
    38341 ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca attatttgac
    38401 acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat acttcatatt
    38461 ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat tagtttagtt
    38521 atatattttt taaagattaa gaatcgaatt atagatgact ataattcata ggaggagata
    38581 taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat tggaagtgaa
    38641 attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag tagaggtgaa
    38701 gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag atttttaatt
    38761 ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga tattattttt
    38821 catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga agctgtaaaa
    38881 acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat
    38941 tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg tgcgacaaag
    39001 ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac agaaacaata
    39061 ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat tcctcttttt
    39121 agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat gacacgattt
    39181 atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt
    39241 ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc tcaatcatta
    39301 ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa agttgtaggt
    39361 atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat tgaacgctca
    39421 tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga cgaactgtac
    39481 aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga attaatgagt
    39541 ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta aggagtttta
    39601 tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat ggaagtctag
    39661 atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg gttaaatttc
    39721 aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg
    39781 ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa ttatctcaat
    39841 ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt ttgtctactc
    39901 cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga tttaaaattg
    39961 gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat gggttgccag
    40021 ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta gattgtttca
    40081 tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt tctgaagata
    40141 ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt gggtactccg
    40201 atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct aaggtaattg
    40261 agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct tctttagatc
    40321 ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt ttaggtgatg
    40381 gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga aaaagcctag
    40441 tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt acaataaaac
    40501 gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa aaagttaaaa
    40561 gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa aaatacttat
    40621 agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga atttactaca
    40681 agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata aatatggatt
    40741 tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc aaagtttgtt
    40801 tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa atggtttcac
    40861 aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata gaggagaaat
    40921 gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata ttcacggagg
    40981 cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa tagcatctgt
    41041 tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg aagattcttg
    41101 gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata gattacctag
    41161 ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat acgttttgca
    41221 agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt tagctttgat
    41281 caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata gtgatgctgg
    41341 tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta tttatattac
    41401 gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta tgatcggaaa
    41461 ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa atttaggaac
    41521 cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa atgaaaatga
    41581 aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt
    41641 taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat tgaatcaaat
    41701 attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt aaaatatact
    41761 agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat tgaaacctta
    41821 agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa aatgcaagat
    41881 ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa aaaaaaaatc
    41941 tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat aaaaaaattg
    42001 aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt ggcaaaagaa
    42061 atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc agatgctgta
    42121 attggagagc atgctataat aaacacaggt agcattgttg agcatgattg tagaattaat
    42181 aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga
    42241 actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc ttggtgtatg
    42301 ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc ttatggcaat
    42361 ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat tatcaagtcc
    42421 tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata attggatagc
    42481 gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata ctggttctaa
    42541 gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct taagactgct
    42601 taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag ctacagtaaa
    42661 tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta atacatggaa
    42721 tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca accaattacc
    42781 taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac gaattttagc
    42841 tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt taggttctac
    42901 ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat ttaacggtaa
    42961 taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa aatatgttga
    43021 gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc aacatagcga
    43081 aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg gacaaatgga
    43141 agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata aggaagcttt
    43201 ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc caaatcgttg
    43261 gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta acgtaataaa
    43321 taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac atttgcaacc
    43381 agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt cagaaaagtt
    43441 atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg aacaagaatt
    43501 tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga acataggaat
    43561 tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac actttaatga
    43621 tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg aaaaggaatt
    43681 tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt
    43741 ttttgtttgt acatccccta taacccatga atttattata aaagaggctt taaatttaga
    43801 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
    43861 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga gaaaagaaat
    43921 tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat accatgtagg
    43981 acagtattta cccgactggc atccttggga agattatcgc aatttttttg tttcaaacaa
    44041 aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta tagatacatt
    44101 tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg atattgatta
    44161 ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa ctttaagtgt
    44221 agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa agattttaat
    44281 agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt ctagtatgac
    44341 agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata ctattattga
    44401 agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc
    44461 ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca ttgaggtaaa
    44521 ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca tctaaaaaat
    44581 ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta tagtagcagt
    44641 ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt ggatacacat
    44701 tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact aaatgtaatt
    44761 gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag tattgatctt
    44821 actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag gtataaaaat
    44881 atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt aagagctatt
    44941 tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa ttattctgcc
    45001 tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga ttatattata
    45061 aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc gaatcttgaa
    45121 attaacagta atgatttagc aacatatata ttgagatacg atgataaaat tatagaaatt
    45181 catttagatt attttggaag agttcctaca agaaaatttc aattgtttac tttagatgaa
    45241 tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat tgttaaagaa
    45301 atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta
    45361 gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct
    45421 gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt
    45481 aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg
    45541 atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac
    45601 agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt
    45661 aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta
    45721 caactagaaa gagaagataa tatatttgat ttaattatag atttagattt gacttcacct
    45781 ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa
    45841 gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga
    45901 attgaaaatg aagttactat agttaataaa tcaagataca catctaggca acagacacct
    45961 gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt
    46021 aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat
    46081 attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat
    46141 tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga tgagctgtta
    46201 tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt taggagaaga
    46261 acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc ttgatatgac
    46321 gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg aagagatcat
    46381 gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg tggcagattc
    46441 agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa tgattttaga
    46501 attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt cagcggctgt
    46561 ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc
    46621 ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc atttatatga
    46681 tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa atccgaattc
    46741 tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa tcgtaggaga
    46801 agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg tttatataga
    46861 tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt
    46921 caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa tcaatgaaat
    46981 tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata tccatgactc
    47041 tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg gtattttaag
    47101 tggaatgact aattatttga aaactgaaat caattaaaac atactaagat aagattagag
    47161 ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca aatctgggat
    47221 agaagggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat ttggacagaa
    47281 cagaaagaag aaaacctctt tctgttctgt ttatttggtt aaaaatgttt tgccatgacg
    47341 aatcataatt cgtagtttga aattgtaaaa gttg
//
LOCUS       DANKHJ010000014        42253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000014 DANKHJ010000000
VERSION     DANKHJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..890)
                     /locus_tag="PW113_000932"
     CDS             complement(<1..890)
                     /locus_tag="PW113_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2525263.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFT"
     gene            complement(1142..1501)
                     /gene="rplT"
                     /locus_tag="PW113_000933"
     CDS             complement(1142..1501)
                     /gene="rplT"
                     /locus_tag="PW113_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2525264.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1554..1754)
                     /gene="rpmI"
                     /locus_tag="PW113_000934"
     CDS             complement(1554..1754)
                     /gene="rpmI"
                     /locus_tag="PW113_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2525265.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1826..2350)
                     /gene="infC"
                     /locus_tag="PW113_000935"
     CDS             complement(1826..2350)
                     /gene="infC"
                     /locus_tag="PW113_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2525266.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(2394..2519)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2670..3302
                     /locus_tag="PW113_000936"
     CDS             2670..3302
                     /locus_tag="PW113_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2525267.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(3304..4209)
                     /locus_tag="PW113_000937"
     CDS             complement(3304..4209)
                     /locus_tag="PW113_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2525268.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(4294..4953)
                     /locus_tag="PW113_000938"
     CDS             complement(4294..4953)
                     /locus_tag="PW113_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2525269.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            5269..6213
                     /gene="mvk"
                     /locus_tag="PW113_000939"
     CDS             5269..6213
                     /gene="mvk"
                     /locus_tag="PW113_000939"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2525270.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            6206..7183
                     /gene="mvaD"
                     /locus_tag="PW113_000940"
     CDS             6206..7183
                     /gene="mvaD"
                     /locus_tag="PW113_000940"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2525271.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            7196..8281
                     /locus_tag="PW113_000941"
     CDS             7196..8281
                     /locus_tag="PW113_000941"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2525272.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            8278..9342
                     /locus_tag="PW113_000942"
     CDS             8278..9342
                     /locus_tag="PW113_000942"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2525273.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9591..10394)
                     /locus_tag="PW113_000943"
     CDS             complement(9591..10394)
                     /locus_tag="PW113_000943"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2525274.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(10414..11256)
                     /gene="kduI"
                     /locus_tag="PW113_000944"
     CDS             complement(10414..11256)
                     /gene="kduI"
                     /locus_tag="PW113_000944"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2525275.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(11272..12279)
                     /locus_tag="PW113_000945"
     CDS             complement(11272..12279)
                     /locus_tag="PW113_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2525276.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(12286..12930)
                     /locus_tag="PW113_000946"
     CDS             complement(12286..12930)
                     /locus_tag="PW113_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2525277.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(13030..13788)
                     /locus_tag="PW113_000947"
     CDS             complement(13030..13788)
                     /locus_tag="PW113_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2525278.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(13915..15066)
                     /gene="fucO"
                     /locus_tag="PW113_000948"
     CDS             complement(13915..15066)
                     /gene="fucO"
                     /locus_tag="PW113_000948"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL2525279.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(15148..15459)
                     /gene="rhaM"
                     /locus_tag="PW113_000949"
     CDS             complement(15148..15459)
                     /gene="rhaM"
                     /locus_tag="PW113_000949"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL2525280.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(15461..16306)
                     /gene="rhaD"
                     /locus_tag="PW113_000950"
     CDS             complement(15461..16306)
                     /gene="rhaD"
                     /locus_tag="PW113_000950"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL2525281.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(16320..17576)
                     /gene="rhaA"
                     /locus_tag="PW113_000951"
     CDS             complement(16320..17576)
                     /gene="rhaA"
                     /locus_tag="PW113_000951"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL2525282.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(17573..19033)
                     /gene="rhaB"
                     /locus_tag="PW113_000952"
     CDS             complement(17573..19033)
                     /gene="rhaB"
                     /locus_tag="PW113_000952"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL2525283.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            19186..20055
                     /locus_tag="PW113_000953"
     CDS             19186..20055
                     /locus_tag="PW113_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL2525284.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(20181..21080)
                     /locus_tag="PW113_000954"
     CDS             complement(20181..21080)
                     /locus_tag="PW113_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525285.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(21245..22069)
                     /locus_tag="PW113_000955"
     CDS             complement(21245..22069)
                     /locus_tag="PW113_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2525286.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(22080..23375)
                     /locus_tag="PW113_000956"
     CDS             complement(22080..23375)
                     /locus_tag="PW113_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL2525287.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(23391..23870)
                     /locus_tag="PW113_000957"
     CDS             complement(23391..23870)
                     /locus_tag="PW113_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2525288.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(23876..24904)
                     /locus_tag="PW113_000958"
     CDS             complement(23876..24904)
                     /locus_tag="PW113_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL2525289.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(24921..26027)
                     /locus_tag="PW113_000959"
     CDS             complement(24921..26027)
                     /locus_tag="PW113_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2525290.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(26256..28853)
                     /gene="adhE"
                     /locus_tag="PW113_000960"
                     /gene_synonym="adhC"
     CDS             complement(26256..28853)
                     /gene="adhE"
                     /locus_tag="PW113_000960"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2525291.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(29158..29538)
                     /gene="yajC"
                     /locus_tag="PW113_000961"
     CDS             complement(29158..29538)
                     /gene="yajC"
                     /locus_tag="PW113_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2525292.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(29635..30780)
                     /gene="tgt"
                     /locus_tag="PW113_000962"
     CDS             complement(29635..30780)
                     /gene="tgt"
                     /locus_tag="PW113_000962"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2525293.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            30998..31696
                     /locus_tag="PW113_000963"
     CDS             30998..31696
                     /locus_tag="PW113_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2525294.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(31864..34503)
                     /locus_tag="PW113_000964"
     CDS             complement(31864..34503)
                     /locus_tag="PW113_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2525295.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(35122..36153)
                     /gene="queA"
                     /locus_tag="PW113_000965"
     CDS             complement(35122..36153)
                     /gene="queA"
                     /locus_tag="PW113_000965"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2525296.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            36346..37521
                     /locus_tag="PW113_000966"
     CDS             36346..37521
                     /locus_tag="PW113_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2525297.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            37525..38166
                     /locus_tag="PW113_000967"
     CDS             37525..38166
                     /locus_tag="PW113_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525298.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            38163..39080
                     /locus_tag="PW113_000968"
     CDS             38163..39080
                     /locus_tag="PW113_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2525299.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            39077..39748
                     /locus_tag="PW113_000969"
     CDS             39077..39748
                     /locus_tag="PW113_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525300.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(40289..40777)
                     /locus_tag="PW113_000970"
     CDS             complement(40289..40777)
                     /locus_tag="PW113_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2525301.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(40978..41244)
                     /locus_tag="PW113_000971"
     CDS             complement(40978..41244)
                     /locus_tag="PW113_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2525302.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(41244..41507)
                     /locus_tag="PW113_000972"
     CDS             complement(41244..41507)
                     /locus_tag="PW113_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2525303.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            41983..42168
                     /locus_tag="PW113_000973"
     CDS             41983..42168
                     /locus_tag="PW113_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2525304.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
BASE COUNT        11990 a         9209 c         7709 g        13345 t
ORIGIN      
        1 gtaaataatt tcgcaaactg acgacagatt tctttataac ctccgcgcaa ttttccagta
       61 aacagcaatg cccagatacc tgctagaata aaacctaaga tgattggaag atctaaaaga
      121 acgaccccta aaactggtaa taatacggct aaccatgaga tcattggtgc gtcgccacct
      181 tcagctggta cgtttgctgc cattgcatag cgtttctttt tgttcattga tatattagcg
      241 accgttagta ccacaactaa gcttacaatc atcccaatca taccaatttg gaaatagtca
      301 ttataagtat agctttcagc cgctggattg aaaccagcat aaatcgtttg atattgtttg
      361 aaattgacga tattagcaaa gattccagcc ataatcgagc ccataaatga gaacagtgca
      421 accggtactg aaatacctaa tgacatcaag attggcaaca caataactgc aatagaaatc
      481 actggtccga ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc
      541 atcgtgattc ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg
      601 attccgctat ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc
      661 gatttggcgt actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct
      721 tgcccttcca tcgccgggtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat
      781 acagtagcca tgatggcaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc
      841 atgaaaaaga atgagataac taacaaaata ccaataataa tatcactcat gttattcctc
      901 cgtccatttt gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat
      961 gtagttagcg ctttcattaa agggtgacca taaatgttca gttttcaatt aaaaaacctt
     1021 aattatgcta ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa
     1081 taaaaaaaga ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag
     1141 cttatttagc caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt
     1201 taacagccaa gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt
     1261 gcattaattt tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc
     1321 acaatttacg gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat
     1381 tcattacttg ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg
     1441 ctaattttag cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca
     1501 tgtataattc ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc
     1561 atttttgcta gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta
     1621 cgcaattgac ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg
     1681 aagcgtttca atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt
     1741 ttttgttttg gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg
     1801 tccaacagcc agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga
     1861 acatgctgcg tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt
     1921 cagctaagcg attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga
     1981 aacggataga agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt
     2041 ttgtattgaa gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca
     2101 ctttttgttt tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt
     2161 agtccatgat tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt
     2221 ttgcttgttc tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat
     2281 ctgaaccgat caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg
     2341 ctatggtcat tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa
     2401 taaaaaaacg agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa
     2461 ctatctagcc cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc
     2521 tcaaccatca tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc
     2581 ttccttttct gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc
     2641 tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc
     2701 ttcctcttct tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc
     2761 tgatcaacgg gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc
     2821 atatccaaaa acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca
     2881 tttttgaaaa atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg
     2941 tgtttcatca atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta
     3001 ctttcactga ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct
     3061 ccttcgatca ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt
     3121 ttttgtctga caatcagttg accggttcga actggtttcg aacttctctg aagggattag
     3181 actttacgac taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa
     3241 tcgtcaacca agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact
     3301 agatcaacga atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat
     3361 gatcaaggcg attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc
     3421 gaagaatcct gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt
     3481 catcgtgtca ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa
     3541 aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc
     3601 caactcttct ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc
     3661 taaaagtact acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc
     3721 tgaaagttgc agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg
     3781 acgttcacta ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt
     3841 acgaaacaat aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag
     3901 tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat
     3961 aaaagcgata ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga
     4021 tacaagcagt aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc
     4081 tagagaataa gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc
     4141 tagcacatcg ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt
     4201 ttcccgcata ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa
     4261 taaaggttta ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat
     4321 ctctgctaat ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac
     4381 aaaattatag cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca
     4441 ttgatcgtaa cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa
     4501 ttgttcatac gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg
     4561 aatatgaact aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat
     4621 gttttccagc agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc
     4681 atttagatgg aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc
     4741 tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa
     4801 agcatactgt tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc
     4861 ataaaatgcc tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc
     4921 accaattgta cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa
     4981 tattgtgttt aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta
     5041 tttttttcta gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc
     5101 tttagaaaac gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat
     5161 gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg
     5221 ctttattttt tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat
     5281 ggccaaggag aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga
     5341 gaaccagcga ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg
     5401 gatgcaatgg acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct
     5461 catgcattaa aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa
     5521 aaaaacttgc aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc
     5581 gctgcagtcg ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct
     5641 cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa tcgcccatgg taatcctagt
     5701 ggaatcgatg cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct
     5761 ttcgactact tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa
     5821 ggacaaacaa gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa
     5881 actggacaaa tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt
     5941 gaaaattcac cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag
     6001 ctgacaatca gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca
     6061 ttgggtgcca aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca
     6121 aaaacaaaag cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg
     6181 atacaaggat taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat
     6241 tgctctaatc aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag
     6301 cctttcgtta acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta
     6361 tatggtagat gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt
     6421 cagtcagttt cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat
     6481 cagtcagaat ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc
     6541 tttagcaggc gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag
     6601 atttgctcga cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg
     6661 ggaaaaaggc catgacgact taagttctta cgctaagcca gtcccttccg attctttcga
     6721 agacgattta gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag
     6781 aaatgggatg cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt
     6841 tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg
     6901 agaaacgatg gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc
     6961 attcacttac tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa
     7021 gcaaggcatt ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga
     7081 aaacagccat ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt
     7141 aattactgca catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat
     7201 tgaagtatct gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg
     7261 ccatccagca gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg
     7321 aaaagtcgga agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg
     7381 caacggagaa ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat
     7441 tcgcttgact gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa
     7501 agtaacaagt gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc
     7561 cgtgaccgtt gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt
     7621 ggagattttc aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg
     7681 cgacatcgct gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg
     7741 gctccaagaa caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg
     7801 tctatccatt gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg
     7861 tagccctgcc tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat
     7921 ggtggcttat cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg
     7981 gtttaaagaa aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact
     8041 gcatgattta tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg
     8101 taatttagct gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg
     8161 cggaatcgta attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa
     8221 agcagaaatc actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg
     8281 aatcgaaaag acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa
     8341 tttgacttcg tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat
     8401 ctaagtacac aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact
     8461 ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca
     8521 gatttgatga ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat
     8581 acttatacga ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca
     8641 gggacttctg tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag
     8701 attcatttga atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat
     8761 tggaaagtac ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa
     8821 gtcggttttg gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca
     8881 gttgatatca gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc
     8941 aagcgggaac tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa
     9001 gcaaatgaag cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat
     9061 gacatattta aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg
     9121 acgcatctga tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa
     9181 gagcttcgtc tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa
     9241 tctctgatct tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg
     9301 aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     9361 gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt
     9421 cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt
     9481 ctgtcccagc ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt
     9541 caatccgctg atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt
     9601 tttccgatat aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca
     9661 gatgcgctag aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt
     9721 gctgctggcg tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt
     9781 gtttcgcgta atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat
     9841 tggatattgt attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct
     9901 tttgccgcag cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata
     9961 ttgatgattt taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg
    10021 aatggggcat ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc
    10081 atcggtgtcc gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct
    10141 tctttgatct gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa
    10201 ccttttgcat cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga
    10261 ttcaagttat taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat
    10321 ccaataccat agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc
    10381 atactaaatt ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat
    10441 atccatgtct gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct
    10501 tgtacctaca cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac
    10561 aactaaatgt tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt
    10621 ggcataatca aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt
    10681 attccaagcg cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca
    10741 tacatttgga tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt
    10801 ctccattggt tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg
    10861 aacagaacta atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg
    10921 tcgtgtttct tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc
    10981 gatctcgatg aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa
    11041 gtaatcaaca cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac
    11101 tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa
    11161 taccttttct actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc
    11221 tggactgtgt gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat
    11281 gatttttcca gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca
    11341 gtccccatgg atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg
    11401 cggagacata ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc
    11461 tactcgatcc acgattgggg tgattctatg gacttgggat tccacatatt catggttcat
    11521 ccacaatgtc cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata
    11581 aaacacctga atattcggga acttttcctg cattttttga taataataaa ttagaggttg
    11641 ttcttctgct tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc
    11701 tgagcagata tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt
    11761 ccataatttt ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc
    11821 ttctatcacc tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga
    11881 aacatgaaat atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat
    11941 ttcagcaaaa cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc
    12001 agtttccata taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac
    12061 tccataacat tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt
    12121 agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc
    12181 gccataatgg gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa
    12241 tcgcaacatg atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc
    12301 tttcattttt gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc
    12361 tgcgggtgct aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc
    12421 tttcatattg tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc
    12481 cttgaaggca gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc
    12541 taccccgctt ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg
    12601 aatttgatac agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata
    12661 ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc
    12721 aatcactatt tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc
    12781 ttgagggact gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca
    12841 agcattcaaa gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt
    12901 caatcgagat aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt
    12961 tattttataa cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg
    13021 cttttttcat tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact
    13081 gattatttgt tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc
    13141 accaatgatt tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat
    13201 ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg
    13261 taatgcattg gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc
    13321 agtgtactgc tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa
    13381 tggtcgggtg attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt
    13441 gttcacgtac aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt
    13501 ttgctgcagc tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt
    13561 tttattcgcg tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt
    13621 tacgtagccg atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc
    13681 cacacccttt gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat
    13741 tttagaggct ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc
    13801 tatttcttta taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat
    13861 ataaactacc atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat
    13921 caaactttga tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt
    13981 gcatacatct cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa
    14041 ttcagaaatg gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc
    14101 cgcttctctt acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc
    14161 gatctcacgg tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag
    14221 tgctgcacac gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc
    14281 gtgtaccagt cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc
    14341 cattttttct cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat
    14401 ttcgattgct tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc
    14461 ttcaatagca tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc
    14521 catcatcata tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac
    14581 aaattttcgg tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt
    14641 tccagaagtc gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc
    14701 ttctaaacta atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc
    14761 cgtatcgatt ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac
    14821 aaaagccact ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa
    14881 agcataagcg attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc
    14941 gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg
    15001 gacgatcgac tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt
    15061 actcattttc tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca
    15121 agcaaccggt tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact
    15181 gactggagag gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg
    15241 attgatagat gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc
    15301 aaataacaaa cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg
    15361 aagtgctgta cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt
    15421 ttctgggtaa actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat
    15481 gaacttcctc caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca
    15541 ggttttcgtc cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt
    15601 ctgctgcctt ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc
    15661 cgtagattcc atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg
    15721 cttccccaat ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa
    15781 aaacaactaa gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta
    15841 attcatgcgt aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat
    15901 tatctgggtc tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg
    15961 taggaaggca ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa
    16021 ctcgaacgat tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg
    16081 aaccagttac aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc
    16141 ggcggatcac tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag
    16201 aaatattccc gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga
    16261 tcatttcttt cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc
    16321 tattttctta attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa
    16381 ccaactggaa cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct
    16441 ttcaattctt ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc
    16501 aatcgctcaa ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg
    16561 atgacccaag cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt
    16621 ttttccaaca attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc
    16681 aaaaccacat ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg
    16741 ctgaataaag ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca
    16801 gcatctagac aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa
    16861 ccgactgtat aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca
    16921 taccgttcat ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt
    16981 ggggtaaaac gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata
    17041 cttgtttgcc cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt
    17101 tcgatccagt attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc
    17161 attggatgcg aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac
    17221 tctacccatt tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta
    17281 tccaaataga tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc
    17341 tccagatctt gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc
    17401 atgattccac cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc
    17461 cagcaatgca tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca
    17521 ccgatagttg catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga
    17581 ttactccttt gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt
    17641 aaaaagtttc acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa
    17701 actgacctgt actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc
    17761 ctgcttctac aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg
    17821 atccgccccc tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga
    17881 tcagctcttg tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg
    17941 atgagacagg gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat
    18001 ttgggggatt caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt
    18061 ttgcagcaag ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt
    18121 cctggatcaa ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc
    18181 attcattcgt ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa
    18241 tccctaaaag cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg
    18301 ttcctaatac agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca
    18361 aatcatattt tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca
    18421 atggtggaaa taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt
    18481 ttgtccctgc attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac
    18541 cagtaaagac ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat
    18601 tttctttttc ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg
    18661 tctgctcata caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg
    18721 tacgcttgtc ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac
    18781 agtaatcgac tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta
    18841 atttttcaaa tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac
    18901 cgtctttgtt ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt
    18961 ttgcatccct cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa
    19021 atgttggatt catttctcca cctcttttac tttattgttg attagcactt tattaataaa
    19081 gcgttatcat ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta
    19141 ttttggtata taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa
    19201 aaagccaacc actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga
    19261 agcaggaatt tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac
    19321 tatactgaac gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt
    19381 tcgcttacat ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac
    19441 tgccaagaat atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc
    19501 catttgaatg gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac
    19561 accttccatt caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg
    19621 atccacatgc aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac
    19681 gagttgctga atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt
    19741 cccgcctacg tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca
    19801 cttgatcaat tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc
    19861 aaatttatcg gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa
    19921 gaccttcttc ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag
    19981 aattttgctt actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt
    20041 cgtaaattca gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata
    20101 tagataaaaa taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg
    20161 ttatttttta gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt
    20221 ttctgtgtat cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct
    20281 ttcagaaaat gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg
    20341 tattctcgca acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac
    20401 gtaaatccag tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc
    20461 ttttccattt cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt
    20521 tgttccacac gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt
    20581 acaacttgat tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca
    20641 ccgtaaattt ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc
    20701 tgttctattt gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga
    20761 ttagaccatg gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc
    20821 tcgaatacat ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc
    20881 acacttcctg ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt
    20941 aatgtagcat ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg
    21001 acatctttga catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg
    21061 ctgattttct tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt
    21121 ataccacgtt ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag
    21181 acgtggcatc ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt
    21241 tacattattc aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa
    21301 ccacatacag gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca
    21361 caccgtctcc caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat
    21421 gaaccgcaca tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt
    21481 cttgataatc actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg
    21541 aatctactgc ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac
    21601 ctggttctat ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa
    21661 cggacacagc ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg
    21721 gcaaaacaca tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa
    21781 gatactgctc gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat
    21841 tgatatagca acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat
    21901 aatttcccat cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac
    21961 ctaaggcgag ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta
    22021 tcgtttggac ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc
    22081 taactcagca tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag
    22141 acgactaaga taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg
    22201 cctcctacac ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca
    22261 cacagattca tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg
    22321 attggtaaaa atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc
    22381 accagtaata acacaagatt gactaataac agaataataa atttattatc cgttacgccc
    22441 ataatcaacg aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg
    22501 gaagaagtag ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata
    22561 ttaggtatat cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa
    22621 atgacagcta tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata
    22681 atgatgacaa tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc
    22741 atctgtcctt tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc
    22801 atgattccta gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc
    22861 gaagcaccac cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata
    22921 cctgtcggag ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc
    22981 acttgctgag ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag
    23041 atggaaccaa ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc
    23101 ccgacgaaca acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata
    23161 atgcccgaga gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc
    23221 attttttgag ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc
    23281 atcgatgaga atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat
    23341 acaaaaaata acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt
    23401 tcttgaattg ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg
    23461 atgagtaccc ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa
    23521 ggcgagatct gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc
    23581 gcagcaaata ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca
    23641 gttaattttt cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg
    23701 gaagacaaca tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt
    23761 ggatttccta aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca
    23821 agcaaaacag ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg
    23881 ctccttgctc ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat
    23941 catgaagtgg ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc
    24001 catgttcttc ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt
    24061 caaaaaccgt tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct
    24121 gttctttgat ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg
    24181 aatagtattt agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg
    24241 atccgttgat caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg
    24301 gcgaaccgcc taaaagctct accattttga tagcggtttc gctttgcatc acacggatct
    24361 tctttccctt caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct
    24421 gaccagaatc ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg
    24481 gctccattat ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat
    24541 aaggaactga aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt
    24601 ttgtgaaatc tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt
    24661 gtccatctgg gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc
    24721 caaattcatt cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg
    24781 taactatctc atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc
    24841 cgcttagacc tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt
    24901 tcatatgggt ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc
    24961 gttaacgact gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg
    25021 tcccccattc ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca
    25081 agcaatcctt ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta
    25141 tggaccgctt tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa
    25201 gaaggatcat ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca
    25261 cgcacttgtg cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat
    25321 tctaagattt caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa
    25381 aatgcttcgg catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt
    25441 cgtttatcca tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat
    25501 tctgggcgat tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta
    25561 tcgtcccaca actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca
    25621 tttgtccgag gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag
    25681 cgtgaatctt tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg
    25741 acgtttttgg gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga
    25801 taagcgcgag gattttttgt catctggtaa tttttataaa taccatatag accgacacct
    25861 tgtggccaat tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac
    25921 aaaaattcac cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt
    25981 tctttcacta attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct
    26041 ttttaaacgc tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc
    26101 gctttttgaa caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc
    26161 agttacttgg aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat
    26221 ctattttctt atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg
    26281 atctgtttca attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg
    26341 taagctaatt cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga
    26401 gcttttagac tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa
    26461 gcttcgacta attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg
    26521 taatctgtat ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct
    26581 tttgcattat agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc
    26641 ccgtattccc cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca
    26701 aacgccatac cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt
    26761 gtttttactg atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca
    26821 cgtgtataat ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc
    26881 atcccggtat ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt
    26941 gcgacatctg gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca
    27001 gtgatgaccg caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg
    27061 aattgtgttt tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt
    27121 tgttttgcac caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct
    27181 gcgtccattg cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc
    27241 agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact
    27301 tcgatcttca catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg
    27361 aattggacca taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct
    27421 aagtacaata gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg
    27481 ttatttctcc gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga
    27541 gaaacagagt tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta
    27601 tagatatcac cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc
    27661 attcgaagac cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt
    27721 cctaatcctc caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta
    27781 gctttgatca tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct
    27841 acgccttcca attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat
    27901 tttgcgattt ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg
    27961 ttcatcactg catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt
    28021 gcttggaatt ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca
    28081 gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt
    28141 ttaatttttg ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct
    28201 ggttttcctg tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc
    28261 gcgatacctg gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat
    28321 tggatacaat tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct
    28381 tctgcagaag atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc
    28441 aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg
    28501 caaaccacac cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc
    28561 acaccaactg ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt
    28621 ttgattgctt tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat
    28681 ggcatatgtt gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct
    28741 tgatcaaagc tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc
    28801 attgcttgta cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc
    28861 ctcctaatgg gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt
    28921 tttctatgta aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct
    28981 ttatttacag gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca
    29041 cacccgatta ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat
    29101 aaaaaatgag gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta
    29161 tttttcttcc ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac
    29221 tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt
    29281 ccctggtttg accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc
    29341 taaaacaact gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat
    29401 cgttacgact gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg
    29461 tttcttttgc gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag
    29521 cattggtaat gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta
    29581 gcagaaaaat gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa
    29641 gctttttgcg ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag
    29701 caaattatca tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa
    29761 attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat
    29821 ataggcacgt gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa
    29881 atcatgggca tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc
    29941 atttcgagcg attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc
    30001 atcgatcaat gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat
    30061 caacggtgta gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa
    30121 cccgccgatt gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg
    30181 aagatcttca aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc
    30241 atgagctttc aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta
    30301 gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa
    30361 tttattttgg atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg
    30421 atttttgaaa tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg
    30481 aaagccgcct gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc
    30541 agcttcttct actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat
    30601 aacgccagca cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt
    30661 tcctactggc atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa
    30721 acgcgcgcca gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat
    30781 tcatttccct actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa
    30841 gtgctttttg caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc
    30901 tttttttatg aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg
    30961 gtatattggt gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa
    31021 acgccaagca aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat
    31081 ccctacattg attggtattg ctactattct tcttattatg attcctatta tcttgtttgc
    31141 caccaccata tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag
    31201 ttcaggaggc ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat
    31261 ttcttggact tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga
    31321 tgcatttcgt ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat
    31381 ctcgatcttt acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta
    31441 ttcttattct caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc
    31501 taaagtactg gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact
    31561 tttctggtta gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat
    31621 tggttatcta tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt
    31681 gccaaaagat atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg
    31741 tttatcgaga ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca
    31801 ttcatgtcac acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt
    31861 taatcattcc tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc
    31921 gattggtacg atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag
    31981 gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag
    32041 taacgccatt tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct
    32101 gctgcgtaca ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt
    32161 ttgaccgaca tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa
    32221 ataataacca aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa
    32281 taaacctcga gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg
    32341 ttccgctggc tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa
    32401 cagtagttgc acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat
    32461 aaagatctcc gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata
    32521 aaccgaacgt ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat
    32581 catatctgcg gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt
    32641 acttgctttc aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc
    32701 cgtacgttgc caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac
    32761 agagagagct ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc
    32821 tgaaagtgct tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt
    32881 caccacatga tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat
    32941 tgctccttta ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa
    33001 gtctttttcc agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc
    33061 aacagcaatc actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc
    33121 tacatcgcct gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat
    33181 gtatttttcg cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag
    33241 ctcgtttact cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac
    33301 agcccttacg atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg
    33361 ctcgaccgtc acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt
    33421 cattgaatct tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat
    33481 ttcattttgt gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt
    33541 ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta
    33601 tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga
    33661 aatcgaagtc atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac
    33721 taaagccgca gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac
    33781 ctgtaaaggt gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc
    33841 tgtagccatg ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc
    33901 tttaaatacc atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc
    33961 atctttttca actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga
    34021 tgcttcgatc aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc
    34081 acccactacc agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc
    34141 cgtttgttta ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac
    34201 aatcgccaat accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc
    34261 cccatgttct gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat
    34321 cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc
    34381 atcgaattta ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc
    34441 agtatctgga tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc
    34501 catcctattc ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc
    34561 ctttttttat ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt
    34621 taaagtgctt atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga
    34681 ggataccatc aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt
    34741 tagaagtaca ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc
    34801 gtatggatgt ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg
    34861 aataacgtaa acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt
    34921 aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga
    34981 tggaaagtta caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct
    35041 tatcgagagc tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa
    35101 aactagcggc ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg
    35161 atagcgctct tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc
    35221 actaactaac atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac
    35281 cactttgaat tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat
    35341 ttctccatta aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc
    35401 cacgattttc ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa
    35461 acggtagaat tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa
    35521 tgtacctaac cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc
    35581 atcaagtaat tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc
    35641 tttcgcatag acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg
    35701 caatggcatt tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc
    35761 aatgatccgt ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc
    35821 aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt
    35881 atctccttgt gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc
    35941 tccatataaa cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg
    36001 taactcttca atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa
    36061 aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg
    36121 caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct
    36181 tcatcattct atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt
    36241 tttgttttcc taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact
    36301 agtaatagat agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa
    36361 aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt
    36421 gaaaagggtg agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg
    36481 cggatgatca accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc
    36541 atccaagaaa ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt
    36601 ggattgatgc ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa
    36661 gtcagtcaag aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc
    36721 cgtgagatgt tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga
    36781 gtcgttcgtg cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg
    36841 cttgatccaa tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg
    36901 ggaaaaacga tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt
    36961 atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt
    37021 catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag
    37081 ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa
    37141 aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt
    37201 accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga
    37261 ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc
    37321 gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc
    37381 gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc
    37441 tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct
    37501 gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt
    37561 ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc
    37621 attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt
    37681 ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg
    37741 ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg
    37801 attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca
    37861 aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg
    37921 ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt
    37981 gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac
    38041 caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat
    38101 tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa
    38161 gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt
    38221 gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa
    38281 tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag
    38341 ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga
    38401 tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat
    38461 tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg
    38521 agaaaagata cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc
    38581 ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg
    38641 tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt
    38701 atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg
    38761 gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg
    38821 atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac
    38881 catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag
    38941 aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg
    39001 aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc
    39061 actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta
    39121 ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc
    39181 tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag
    39241 gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc
    39301 tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt
    39361 tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca
    39421 agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact
    39481 attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt
    39541 agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga
    39601 tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt
    39661 gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct
    39721 agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc
    39781 atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga
    39841 ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat tccgtatact
    39901 ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc
    39961 cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga
    40021 tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg
    40081 tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca
    40141 tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg
    40201 ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt
    40261 tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg
    40321 cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc
    40381 aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac
    40441 tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt
    40501 tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg
    40561 tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact
    40621 ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat
    40681 ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta
    40741 acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat
    40801 aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag
    40861 gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga
    40921 gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca
    40981 ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta
    41041 aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag
    41101 cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc
    41161 aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt
    41221 gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta
    41281 aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga
    41341 aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca
    41401 ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt caataactga
    41461 taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct
    41521 ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact
    41581 taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga
    41641 tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat
    41701 tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca
    41761 acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg
    41821 aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc
    41881 tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg
    41941 atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt
    42001 aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta
    42061 agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga
    42121 ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata
    42181 ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga
    42241 ataagacaaa aca
//
LOCUS       DANKHJ010000015        41707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000015 DANKHJ010000000
VERSION     DANKHJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="PW113_000974"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="PW113_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2525305.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="PW113_000975"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="PW113_000975"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2525306.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="PW113_000976"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="PW113_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2525307.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="PW113_000977"
     CDS             2179..2499
                     /locus_tag="PW113_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2525308.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="PW113_000978"
     CDS             complement(2611..3480)
                     /locus_tag="PW113_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2525309.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="PW113_000979"
     CDS             complement(3576..4880)
                     /locus_tag="PW113_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2525310.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="PW113_000980"
     CDS             complement(4877..5386)
                     /locus_tag="PW113_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525311.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="PW113_000981"
     CDS             complement(5561..7696)
                     /locus_tag="PW113_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2525312.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="PW113_000982"
     CDS             complement(7779..9434)
                     /locus_tag="PW113_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2525313.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="PW113_000983"
     CDS             complement(9436..9750)
                     /locus_tag="PW113_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2525314.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="PW113_000984"
     CDS             9920..10180
                     /locus_tag="PW113_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2525315.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="PW113_000985"
     CDS             10194..10508
                     /locus_tag="PW113_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2525316.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="PW113_000986"
     CDS             complement(11238..11792)
                     /locus_tag="PW113_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2525317.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="PW113_000987"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="PW113_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="PW113_000988"
     CDS             complement(14153..14869)
                     /locus_tag="PW113_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2525318.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="PW113_000989"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="PW113_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2525319.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="PW113_000990"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="PW113_000990"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2525320.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="PW113_000991"
     CDS             complement(18339..19799)
                     /locus_tag="PW113_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525321.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="PW113_000992"
     CDS             complement(19789..21084)
                     /locus_tag="PW113_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2525322.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="PW113_000993"
     CDS             complement(21416..22093)
                     /locus_tag="PW113_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2525323.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="PW113_000994"
     CDS             complement(22123..23214)
                     /locus_tag="PW113_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2525324.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="PW113_000995"
     CDS             complement(23315..23749)
                     /locus_tag="PW113_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2525325.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="PW113_000996"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="PW113_000996"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2525326.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="PW113_000997"
     CDS             complement(25787..27682)
                     /locus_tag="PW113_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2525327.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="PW113_000998"
     CDS             complement(27740..29515)
                     /locus_tag="PW113_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2525328.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="PW113_000999"
     CDS             complement(29565..31061)
                     /locus_tag="PW113_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2525329.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="PW113_001000"
     CDS             complement(31255..32877)
                     /locus_tag="PW113_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2525330.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="PW113_001001"
     CDS             complement(33205..34518)
                     /locus_tag="PW113_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2525331.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="PW113_001002"
     CDS             complement(34763..34987)
                     /locus_tag="PW113_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525332.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="PW113_001003"
     CDS             complement(35007..35429)
                     /locus_tag="PW113_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2525333.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="PW113_001004"
     CDS             complement(35571..36281)
                     /locus_tag="PW113_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525334.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="PW113_001005"
     CDS             complement(36299..36796)
                     /locus_tag="PW113_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525335.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="PW113_001006"
     CDS             complement(36833..37285)
                     /locus_tag="PW113_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2525336.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="PW113_001007"
     CDS             complement(37420..38025)
                     /locus_tag="PW113_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2525337.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="PW113_001008"
     CDS             complement(38475..39095)
                     /locus_tag="PW113_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2525338.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="PW113_001009"
     CDS             complement(39097..39966)
                     /locus_tag="PW113_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2525339.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="PW113_001010"
     CDS             complement(39989..40396)
                     /locus_tag="PW113_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2525340.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="PW113_001011"
     CDS             complement(40415..41704)
                     /locus_tag="PW113_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2525341.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13943 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DANKHJ010000016        41480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000016 DANKHJ010000000
VERSION     DANKHJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1308)
                     /locus_tag="PW113_001012"
     CDS             complement(<1..1308)
                     /locus_tag="PW113_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2525342.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQK"
     gene            complement(1315..2226)
                     /locus_tag="PW113_001013"
     CDS             complement(1315..2226)
                     /locus_tag="PW113_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2525343.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            2346..3203
                     /locus_tag="PW113_001014"
     CDS             2346..3203
                     /locus_tag="PW113_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2525344.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(3390..3752)
                     /locus_tag="PW113_001015"
     CDS             complement(3390..3752)
                     /locus_tag="PW113_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2525345.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            4098..5537
                     /locus_tag="PW113_001016"
     CDS             4098..5537
                     /locus_tag="PW113_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525346.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(5544..8096)
                     /locus_tag="PW113_001017"
     CDS             complement(5544..8096)
                     /locus_tag="PW113_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2525347.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(8210..9540)
                     /locus_tag="PW113_001018"
                     /pseudo
     CDS             complement(8210..9540)
                     /locus_tag="PW113_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(9506..10984)
                     /locus_tag="PW113_001019"
     CDS             complement(9506..10984)
                     /locus_tag="PW113_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2525348.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(11289..13124)
                     /gene="tdc"
                     /locus_tag="PW113_001020"
     CDS             complement(11289..13124)
                     /gene="tdc"
                     /locus_tag="PW113_001020"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2525349.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            13594..15021
                     /locus_tag="PW113_001021"
     CDS             13594..15021
                     /locus_tag="PW113_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525350.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(15113..15478)
                     /locus_tag="PW113_001022"
     CDS             complement(15113..15478)
                     /locus_tag="PW113_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2525351.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(15475..15840)
                     /locus_tag="PW113_001023"
     CDS             complement(15475..15840)
                     /locus_tag="PW113_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2525352.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(16032..16280)
                     /locus_tag="PW113_001024"
     CDS             complement(16032..16280)
                     /locus_tag="PW113_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525353.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            16279..16407
                     /locus_tag="PW113_001025"
     CDS             16279..16407
                     /locus_tag="PW113_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2525354.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            16464..18158
                     /locus_tag="PW113_001026"
     CDS             16464..18158
                     /locus_tag="PW113_001026"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2525355.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            18162..18281
                     /locus_tag="PW113_001027"
                     /pseudo
     CDS             18162..18281
                     /locus_tag="PW113_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            18258..18431
                     /locus_tag="PW113_001028"
     CDS             18258..18431
                     /locus_tag="PW113_001028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525356.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            18809..19942
                     /locus_tag="PW113_001029"
     CDS             18809..19942
                     /locus_tag="PW113_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2525357.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            19973..20293
                     /locus_tag="PW113_001030"
     CDS             19973..20293
                     /locus_tag="PW113_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2525358.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(20322..20786)
                     /locus_tag="PW113_001031"
     CDS             complement(20322..20786)
                     /locus_tag="PW113_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525359.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(20918..22171)
                     /locus_tag="PW113_001032"
     CDS             complement(20918..22171)
                     /locus_tag="PW113_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2525360.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(22200..22517)
                     /locus_tag="PW113_001033"
     CDS             complement(22200..22517)
                     /locus_tag="PW113_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2525361.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(22543..22863)
                     /locus_tag="PW113_001034"
     CDS             complement(22543..22863)
                     /locus_tag="PW113_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2525362.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(23327..24742)
                     /locus_tag="PW113_001035"
     CDS             complement(23327..24742)
                     /locus_tag="PW113_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2525363.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(24759..26075)
                     /locus_tag="PW113_001036"
     CDS             complement(24759..26075)
                     /locus_tag="PW113_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2525364.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(26225..26923)
                     /locus_tag="PW113_001037"
     CDS             complement(26225..26923)
                     /locus_tag="PW113_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2525365.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(27212..28168)
                     /locus_tag="PW113_001038"
     CDS             complement(27212..28168)
                     /locus_tag="PW113_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2525366.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(28281..29114)
                     /gene="msrA"
                     /locus_tag="PW113_001039"
     CDS             complement(28281..29114)
                     /gene="msrA"
                     /locus_tag="PW113_001039"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2525367.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(29289..30923)
                     /locus_tag="PW113_001040"
     CDS             complement(29289..30923)
                     /locus_tag="PW113_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525368.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(30910..32778)
                     /locus_tag="PW113_001041"
     CDS             complement(30910..32778)
                     /locus_tag="PW113_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525369.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            32744..34381
                     /locus_tag="PW113_001042"
     CDS             32744..34381
                     /locus_tag="PW113_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525370.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(34526..35266)
                     /gene="truA"
                     /locus_tag="PW113_001043"
     CDS             complement(34526..35266)
                     /gene="truA"
                     /locus_tag="PW113_001043"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2525371.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(35457..36092)
                     /locus_tag="PW113_001044"
     CDS             complement(35457..36092)
                     /locus_tag="PW113_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2525372.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(36239..37480)
                     /gene="sstT"
                     /locus_tag="PW113_001045"
     CDS             complement(36239..37480)
                     /gene="sstT"
                     /locus_tag="PW113_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2525373.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            37678..40707
                     /locus_tag="PW113_001046"
     CDS             37678..40707
                     /locus_tag="PW113_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL2525374.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(40767..41459)
                     /locus_tag="PW113_001047"
     CDS             complement(40767..41459)
                     /locus_tag="PW113_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2525375.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT        12982 a         8275 c         6843 g        13380 t
ORIGIN      
        1 tttttgaaca gctagtgcat ctaattcttc gacaaagact ggttttttat ttgaaccata
       61 tgtttcaacc atttgaacaa ctaatttttg gcctttccct aatggatcat cattgtcgcg
      121 aagtgattgc catgctggtg tagaatgacg acgaccgcca ggattatgtc ccatgttagg
      181 cgcaccgcca aaaccagata aacgtccttt tgttacggta gaagagtttc cttcgtaatc
      241 taattgcaag gttgaaccga taaacatatc gatggcatat tgtcctgcag cttgtgataa
      301 tgttcggtta ctgcgcatcg tgccgtcttt accgacaaaa aagacatctg ggcgagcagc
      361 aatatatttt tccataccaa cttcgccacc aaaactatgg atgctttcaa cccaacctga
      421 ttcgatggct ggaatcatcg ttggatgtgg gttcaatacc cagttcttcg cgattttccc
      481 ttttaaccct agtgattcac cataagttgg cagtaacaat tcgatagctg ctgtattgaa
      541 tccaataccg tgattcaaag attgtacgcc gtgttctgcg tagattcctt tgattgccat
      601 cattgccatt agaatttgca attctgtgat gttttgagga tctcgtgtga ataaagcttc
      661 taattgataa ggtttatctg ctttcacgac aacgtctacc cagtcaccag ggatatctac
      721 acgtggtaaa gaatcgacaa cttcgttaac ttgaacgatc acgataccat ctttgaaagc
      781 tgcagcttcg atgattgttg gtgtttcttc cgtattgaaa cctgtgtaca agttgccgtc
      841 tttatctgct ttatctgcag ctactaaaac aacatttgga atcaaatcga tgaataagcg
      901 tccataaagt tccaaataag tatggatatc acctaatttc attgtgccgt cttcaatcat
      961 ttgggcgaca cgcaagcttt gtgcaccagc ataagagaaa tcgatcttgc ttgcgattcc
     1021 tttttcaaaa atattcaaat gttctggtcg tgagatacta gacatgatca aatgcagatc
     1081 attcactttt tctgcatcta ctttttcaag actttctgaa agaaaacttg cttgtttttg
     1141 attgtcgcct tctaatacaa ctttgtcgcc gggagcaatc agtttttcca ttacttctac
     1201 gattttttca gtttctacat acttgccatt gagcaagttg ctgatttttt ccaatcgctg
     1261 cgcttttttt tcgcgtttag aagcccagtt gcggtttggt ttttccataa ttagtcatct
     1321 ccatttttga tatagaaacg tggcgtcatc aataccatgt tggtctagat tgatcgtacg
     1381 gatcccttta tctacaccaa attttgtatg aatcagcttt gtcaaagtat tgccgggggg
     1441 aaattcaatc gtcagatcca ttcctaattc ttttgccaag tccatcattt ggttccactg
     1501 cacagggaaa gctaggtttt ccaacaaatc tttacgaatc tcttctgcgt cctgagtggc
     1561 tcttccatcc gtatttttta tatacaaaca tctagcatca gatagatgga cagactgcat
     1621 atacggcttg aatttcttga ccgtatcatt catcaagaga cagtgagatg gcacagggac
     1681 tttcagcagc ttcgctgttt gcgcttggtg ccgtttggct tctgccaacg taacggataa
     1741 accttcgata ctcccagata acgtatgttg catcggacaa ttttcatttg acagataaac
     1801 tggatggtca ggatcaaatg tttttgctac aatttcctca gcttcgcttc tcgtcacacc
     1861 tactactacg cccattgcat aaccgcttgg ataagcttct ttcattaaga ctgcgcggtg
     1921 atagactagt tccgctgcat tctcaaatct caaagatcga caagcaacag ctgcacaaaa
     1981 agcaccaatc gaatgtcctg ctgcaatatc aggctgaata ccgttttttt ccatttcatc
     2041 taaataaaat actgcttttg tcagtaatgc tagctggata aaaaccgtat cttgatagtt
     2101 ttcttcttta tcaactaacc gataccccgt cacattttct aaacgttcca tgtacatttc
     2161 agggatatcc gctaacatcc ctaacttctg actgccttgt ccaggaaata agaaacctgt
     2221 actcatgagc ctcgcctcct cagcacttac tttttccact gctatcttag tcaatttctg
     2281 ctataataaa aaatgccaaa aaaagaaaaa accataacaa aaaggtatgg atgggatgga
     2341 taattatgaa attgcttcat ctgcagtatt ttaaaaaagt attcgaaaca aaaaatgtta
     2401 cccaagcagc caaagaatta tttatttctc aacctgcctt gagccgtgcg atcaaacatt
     2461 tggaaaacga attaggtgtc cctttgtttt accatagcgg cagaaacatt gagacgaccg
     2521 tttatgcaga agaattttat ccatatgcag tcaagacatt agacacatta gaagaaggaa
     2581 tcaaattatt gtcttcggtc aatgagaaga tcgttacttc cgtcatattg tatttagagg
     2641 tagcctctgt ttctattccg aacttggtcc gaatttttat ggaaaagcat cctgatatcc
     2701 aattaacgat catgcaacat catttaccag atgatattac cgatcctgta ctgtacatca
     2761 cttcggagat gaagcctggc atgaccaata ttcctatcat gaaagaacct gtttatgtcg
     2821 ccattcctaa gaaacatcct cttgctaaaa aagaacatct gcaattagaa gatatccaac
     2881 gcacccccat cttgatgctg tcaaagagca acgccttccg tcatacactg gatgcagcac
     2941 ttgaagcgaa aagtatcgaa ttgacgatca gttcgacgac agatgatcct gcgacattac
     3001 gttctattgt caagcaagga ttagctgtta gctttttccc aaaagtttct tggtcttacg
     3061 ataaaaatga tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca cgaacaattt
     3121 atttatcttc tagcttcaca gaagatcatc ctttgatcaa aatgattgca aaaacgatgc
     3181 gggaatttta tttaggaaaa taaaaaaaaa agagctggga caaaattctt gtcgcagctc
     3241 ttttttcgaa caaacggtgt tccagaagta atttcttaga aaataagccg aaatttccca
     3301 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctaa acatttctgt
     3361 cacaacctct tctctattgt cctagacatt cattgcgttg atttaaaact tccatcaatc
     3421 aaatctgcta gttcatctag cttttctatt ttacgtagat caatagagat ccaatgttct
     3481 ttattcatgt gataggctgg aaaatacccc tcttttcctc gcaaaattcc gccaagttca
     3541 ggatcgacct tcaaatcaag aatattgatg atcctatggt cacttgcatc atcatagatt
     3601 ttttcttctg gaacagacat caggacccca aaccattttt tgcttttttg atgtctgaat
     3661 acgcaataat caggaaattt tttaaaaata tattctggag acaaattgta tgtttcattg
     3721 atgtgatcga ttagttcttt tttcgttagc atctgcttcc atctctttct tattgattcg
     3781 ctttattcaa agataccacg taagaataga aaaatgaata caagtagttg ctaatcaaat
     3841 acttcgatat ttttcttttt tatcaactct taaatgttca atccgcctgt gttggtcttg
     3901 ctaccatcta ctattaaaat gtttattatt gtttgcccac ctattttttg attttagtgg
     3961 tatcattttt caagttcatg atgaaattct tatcatcagt aaatgctcca gaaaaataaa
     4021 caatgattga aaaatcagtt gaaacttgac caccgtttat tttctccgta ttcttagaat
     4081 agaagggagt ctgattgatg tattcactaa tgaaaaaaat cattaccgaa aaagatattc
     4141 gacgtcatgt ttctttagta gaacaattgc tgaatcatac aaagataacc gtaaatgaat
     4201 tagctgaaat catagggaca accgaacgaa cgattttttc tgaccttcaa tctattcgct
     4261 ctcagctgcc cgaaggctgg gacatcatca gcgatcaagc aggaatcagt ttgcaaaacc
     4321 agcaaaattt attaacaaat gacctttggg agattttttt caaacaatca gtcagtgtcg
     4381 aactgctgaa aaatctgctt tttacaaaaa aagttgctgt ccctgatttt ctagccgacc
     4441 atgggctttc ttatgggaca ttgaaaagac atgtgactaa aatcaatcag cgtttagcaa
     4501 gttacgactt gcagatcgat ttgacgaaat atacagcttg tattcttgga aaagagcgtg
     4561 cgattcgtac tttttatcat cgattgctga ttcctttcac tcataacaac tattttttcg
     4621 aggattattc gatccatgaa tcccattatt ttcaattttt aagaaacctc agtcaaactg
     4681 aactagcggt tgaaacagaa gagattttcg gaacatgttg gttctttatc aatacgatcc
     4741 gtataaaagc gaattgccga ttagattctt ctatccatac caacagcaca ctctcctccc
     4801 tgtatgactc tgcattgaaa aaattgtatt taacggaagg tatttatctt aaagacactg
     4861 agctttcttt tgcttctttc tgctttctgg aaagttggaa ttacaacaat aactatggac
     4921 aagaaaccgc tcgctgcctg catcattctc catttttaga ggtcctcgaa acgtttgtag
     4981 aggaactagc ttcagagctg tctcttgatc aactaaaaaa aacatctctg actgataact
     5041 tggctctcct catattaaaa tatcatgaat cgccgatatt gattgaagca ttggaccgtc
     5101 aataccatcg ttttttagaa acatatgatc agcaatatgc acacttgtat caacagaaag
     5161 agaatttact gaacgatcta aaaaaagaaa tagaaatcaa tgaaccaaat tactttctcc
     5221 aactcttgtc tctactgatc caacaaacga ttttttctat caaaccgaat ttgttcaatg
     5281 tctattttat tttccaagga gaaccttcat ggaaggcgtt cttgcagcag gaattaaaag
     5341 attacttagg aaaacgtgtc tgcttcttac ctatcgaact aaccgatctt tccactattt
     5401 cttttgaaaa aacagatatc ttgatttcca atttccctct agattctcta gatatcccta
     5461 ttgtatacat ttcttctatt ccaacgaaaa atgagctcaa tcgtttgact gaactcactt
     5521 tcaaaacttt tttatagtaa cggctagcga ctgtattctc tagctgtttt ttcttttttg
     5581 cgaaacagtt tgttcatgat ccctttttct atttctacaa ctaagaatag aagaatcaca
     5641 tttgctccaa tcaataactg ttgtgtcatc gttaaactta ctgtaccgat cagctgctgg
     5701 ccgactggta gaaaaataac catcccttgc aataaagcaa ggatacctaa tgaaagaaaa
     5761 agcgctttgt tttgcaaaag accttggttc aatgacggct tggacaactc tctgcaattg
     5821 atcatgtaaa cagcttgagc taagacgata ttttgcagaa gaatggtttg ctgtaagctt
     5881 tgcccttcaa actgcatagc caataaatag gcaggaacca tgatcagtag ggagacatag
     5941 atgatccgaa acaaatgata accagatagg attccttgct ttggatctct tggtgggcgc
     6001 ttcatgattt ctggatctgg cgactcgaac cctagtgcat aagataaagt gattgtcgtt
     6061 accatattga cccataaaat ctgtactggg gacaaaggta gcgggcgatt cagcataagt
     6121 gcccaaatca agatcaatcc ttgagctaat gcagttggca agaagaaatt gatggttttt
     6181 tgcaaattat ccaaaatccg ccggccctct tttacggctt ttgcaatcgt atggaaatta
     6241 tcatctccta aaaccatatc agctgcctgt ttgcttactt cactaccttt gattcccata
     6301 gcaattccta catcggatct tttcaaagca ggtgcatcat ttacgccgtc tccggtcatt
     6361 ccgacgattt ctccattttt ctgcaaagct ttaacaattc gcagtttatg ttctggtgtc
     6421 gttcgagcaa agacatcgac tattggcact tgctgaatca actcttcatc agacataaga
     6481 tccaaatcga tcccttctaa aactttggca gtatgtttta agccaatttg ttccgcaatc
     6541 gcttttgctg tgtctttgtg atcccctgta atcattttca ctgagatccc tgcttgttgg
     6601 cattccttta cagctttgac tgcactttct tttggcggat cgatcaatcc ggcgatccct
     6661 gcaaaagtta attgggttaa agtctcatgc gtgagttcct gtttcgaatc tactttttta
     6721 taagcaaatc ctaaaacacg ctgacctttt ctcgcaaatt ctgcagctgt ctgactccat
     6781 gcttcttgtt ctgcagaaga aagagttgat ttagcaaata atacttctgg tgcacctttg
     6841 acgtaaatca ccgctccttt ttcctccgca tgcatcgttg ccatatattt gtattcagaa
     6901 ctgaacggaa tctttgcttc tatttttttc aaaggtagat tggctgtttc tgtgtgtcga
     6961 atcagcgcaa cttctgtcgg attgccactt aaattttctg ttttctgttc ttcttttgtt
     7021 tttaattcct gacagttttt cataatggac aacacgcagg cttcttcttt tgcagctaca
     7081 tccacaactg tcatttcatt ttttgtcaat gttcctgtct tatctgaaca gatcactgtc
     7141 atagaaccta atgtttctac cgaaggaagt cctttgatga tcgccttttc ttttgccatt
     7201 tcatgtactc caacggatag aatcatggtc aaaacagctg gcaatccttc gggtaccatt
     7261 gacaccacca aagcaatcgt agccgaaaga aggatatgcc aatccatacc atatcgaaat
     7321 gtcgtaaaaa taactaagaa taggctcaaa caaagaatgc cttgaaaaat ttgtttgttt
     7381 aatcggttga tttttttaat cagaggcgtc gtttgttgtt cgaccgattg caatgcttga
     7441 ttgattttac cgatttctgt cgtgtttcct gtttctacaa cgacaccgac tgcagaacca
     7501 gattgtacaa gcgttcctga aaaacccata tttttctggt cgccgatcga ttcttcttct
     7561 gtcaaaacgg catgactttt ttcagatgct tctgattctc ccgtcaaaat cgattcttcc
     7621 actaataaat catgtgcttc gattaatcgg atatctgctg gaacgacatc tccggcacgt
     7681 aagacaatga catctccttt taccagttct ttcgcaggga ttttttcttg catcccgttt
     7741 gatagtacta tcgcttcttg tcccatcagc tgcttcaaac cattcaatga ttcttccgct
     7801 tttctttctt gccagtaact aacgagtcca ttgacgatca ctacgaataa aataatactt
     7861 ccttcaatat attcactaga agcaaatttc aaaaaagagg caatgatcaa cacgatcatc
     7921 agcagatcag taaaatgctt tgctattttt ttccatcgtt ttgtctgctg ccttgctttt
     7981 atttcattat atccttgatc agtcaggcgt ttctgtcgtt cttcttgcga caagccttct
     8041 gttgatgttt gatagttttt caatacatct tctaccgtca atctgtacca gttcatccta
     8101 tcccctcctg tttaattgat actgttatta tacgcaggga agttttttca ggcgtatcga
     8161 ttatttcagg ggggtctgaa ataattcgtt ttgcaaaatg tatctgggcc taactgatat
     8221 ttctattgct tattctttta tatacaaaat ttaagaatac aaaccagata accaataaaa
     8281 aaggaataaa gcgtgtcgtg ctttcgaata acaagctgaa agcaattgaa gtaaaaaaca
     8341 ccaacaaagc aagatcttta tatctttgtg tagtgttccg tttcttttct ttaataaatt
     8401 gaacatgtgc acacaagatc gaagaccaca caaatagaaa acaactcgtt gtcatgcctg
     8461 ccaaaagctg aaaaaacaat cgactatcag ctacaaaaaa atgtagacaa agaccggcaa
     8521 aaaataacat tcctgtaatt aaaataccgt gtaaaggtac gcctttctta gaaagcgcac
     8581 aaaaccaatg gggcaaactt ttttcttttg ctgtttgata taaaatcctg ctagtactgt
     8641 agactgcact atttgctgag gacaaagaag caaccaaaat cattagattg acaattgctg
     8701 ctccgccttg gcaatggatc acttgcagca tagtcactaa aggactttct tttaaatcta
     8761 aacaatgcca tggaacaaca aataaaatcg acaaaatcgt tcctatatag aaaaagagaa
     8821 tacgccaagg cagacagttg attgcttttt tcaaattctt ttgcggatca cttgtctcgc
     8881 caacggttaa gccgatcatt tcaattccag caaaactgaa taaaaccata ggaaatgaga
     8941 ctaccattcc agcaaagcca aacggtagta attcatcgat ctgtccaaaa ttttctttga
     9001 ttccgataga ctgggtatga aaatctgaga cactcaatcc tatacccaat aagataaaaa
     9061 ggaaaatccc caagatcttg atgattgaga aaaaagattc caattcacca aaccacttga
     9121 ctgcaacaag gttgactgcc attaagatca ttagtgcgct acctccgctt atcaaagctg
     9181 gtacatgtac aaaccagaaa tggatataca atcctattgc tgttaattcc gcaagtccag
     9241 ctccaagcca gcaaagccaa taagtccaat tgacaaaaaa tgcccacttt tgcctaaata
     9301 atagtcaacg aaatcactga tagagttaaa tgtactattt gctaaaaata aatcacccat
     9361 ggcccgcatc aaaccataaa tagccaaacc gcatatcaaa taaacgagaa taacagctgg
     9421 ccctgctgat gcaattgcct ttgctgaacc taaaaataat cctgttccaa ttgcgccacc
     9481 taatgcgatt aactggatat ttcgattagt tagagtccgt ttcattgccg gtttttccat
     9541 caagttcacc tttttctttt tcaagcagca tggcgacttc ctgttcccaa gaaggtttct
     9601 ttattttttc aatacagagt ggtactacaa acaaaatgac actgatggct gtgactgcaa
     9661 ttagattgat ccatgttcca gctttcatcg tcagtccgac agaaaatccg attgccgcaa
     9721 ataaaacaat aacgacagtc caaactccaa gattgctctt tttctctttt tttccaattc
     9781 taaatggtcg attcaacttt gcatgtgtat aacgcaatct caaaactgcg atccccatga
     9841 tgacatagac gatacagtag ataacagtag ttgaatttac gagcatcaaa aatgcttgat
     9901 tgacgccagg aatcagcaaa taaaaaagtg caaagattga gataatgaca gactgagtca
     9961 aaataacgga tttggatacg tcaaattgat tcgtcttcca aaaattgaat tttggtggat
    10021 acgcaccttt tctagcactt tctgtcactg tctttgctgg tcctgaggcc caagcagata
    10081 attggactgc tacaccaatg aagacaagta aactaaacag attcacaata agatgtggca
    10141 aaccaagaat ttggcaataa atcgaaatag attgcgcgat attattcaat tccatttgtc
    10201 cttttggtac gacatttgct acaactaaag cattcaaaat attgacgaaa aatataaaaa
    10261 ttaacgcggc aaatactccg cgaatatacg ttttaactgg attttccaat cgagtgatat
    10321 acacagagga catttcgata ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg
    10381 aggatgcagt cgtagtatct ggtattaatt tgtcccatga aaaactgcct aatgttccgg
    10441 ttggtacgat cccaactttg atccacgcag caaaccctaa taagagcatc atcgtcaaag
    10501 gcacgtacaa tcctaaccaa acaccgactt ttccgccgat ttttgccata tcaaatttca
    10561 aattcaaaaa agtgatgatc caataaacga ctaaaatgac aactaatgta aatttgctgt
    10621 tcaaaccaag cggcacatta tcgataatat taccaaacaa cggagcgagt gctgaagcga
    10681 ccatcaccat cccaggaaac atttggaccc ataacagcca tgagacaacg aacccccatt
    10741 tttttcctag agcattcgat acccaaagtt ctggaccacc tttttgggga aacattccag
    10801 caaattcacc agagatcaat gagattggaa aagcgtacaa taatactgca accagcatgt
    10861 aaaaaatcat cgtccagctc gttgctgcta catctggaat atttcgtaca cttgctacaa
    10921 gggcgcaggt cattccgata aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt
    10981 tcatctttat tccttctttc aatagcgaca tatgtttttg ttttctttta tttttgttca
    11041 agaacagaaa tgacaaggtt tcatcgattc agtacaaaac ccagtatcac cgaaacatcc
    11101 attgaacgat cttagtgaac tgggtttgat acatgaaacg aacgatccaa cttatagaac
    11161 gttttataaa aaagaatatc ttggcgagga atatatgaaa aacaggggga aatacaattg
    11221 ttttgatgaa gcaaataatt gtctgttctc catatgtctc ttgttattct ttcacacaaa
    11281 aatcagttct attggatgat ttcgtttagt ttcttttcca ttgctttggc gatttttttt
    11341 gtataaaaat tgaaaattcg atcatcattc aaataaggag tcatgatagc cgcacgtagc
    11401 agagtgattt tctgttcttt tttccattct tctttcgtta gacccattct ttcaacaaaa
    11461 cgaatcggag aatcaccgta atcttcttgt gtaaatgtag tatgagaagt aataaaccgt
    11521 tcaccataaa catcgccatc catataagaa gaccgatcga acattttttc atttaattca
    11581 ttgatagcgt tcaaatctgt acagccttgt tctttgaata cccaattgac catattaaaa
    11641 tcaggatgat tcaatggata gacttcgatt gtttttcctt taacagtaaa agtcagatga
    11701 tcgagaaatt ctctgaatct ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat
    11761 ccggtcacat tcaatggcaa tacacgatgt gctgtccaga ctgcggcggc ggttgctcca
    11821 gcttttgatc cttccaaaat ataagctcct aacatatctg gagcctttac tgatttttca
    11881 aacacatatg gcgcgaaata agaaatgata tttctcatat ttttatgttt gattacgatt
    11941 cctccagcag cataagggac atagcccatc ttatgaggat ctatcgtgac agaatccgct
    12001 tctgaaatag ctctgaaccc ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa
    12061 acatgatgtt cttcaaagaa ttcagctaag gaagcatacg gcacaaattc tcctgcttcg
    12121 tttagaaaca gtgaacgcgc atagccgcca tatgcggcat caacatgtaa atagaaatag
    12181 atcccttcat cttttaacct ttctcttaat tggacgattt tatctacact gtctacttga
    12241 ccttcttctg tcgttcctac gacagcaacc acacctagaa ttgggatttt ttgtccagct
    12301 aattcacgaa ttgttttttc caaagcatta atatccattc gataatcctc ttgaactggg
    12361 atagcaacca tctgatctaa gccgactccg caaatatcta acgccttcat ccaagaatag
    12421 tgttttgttt gagggaccag ccatttccct aatctttgga tattttttcc gctacgggaa
    12481 gaagctgctt tgacctcatc catttcttcg tccgtaaagc tttcggtcat ttccaaaatt
    12541 tcttcaacag ataaattcaa aagttcccac tcagacattt ttttcacttt ttcaggcagc
    12601 acttccttga cagctaaagg aatcgatttg atgcaacggg cgtaccataa tccttcaaga
    12661 ttagcaatag aaccatctgc tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa
    12721 gcaaaatcct gtcctacttc tgcttccatt tgcgaagttg ccatagaaga ttccaatgca
    12781 acattatttg ggttccacag catcgcatag ttgtaagcta ataaagcagg catcaatgtt
    12841 tcagcattca tttgtcccca gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt
    12901 aatcgctgac tgacttcatc caagacctct atcatatggt cgcgggtggc tagataatct
    12961 ggagtgagct tatctccttc gctaattgca ggcatatctg aaggaatata attttctctc
    13021 catccaagat gttcatctac catcttgttc aacaacattt tataaactgg tccattttca
    13081 gctttgtcgc ctataaagac ggccttgata tccatatctt tcatttttct tccctccata
    13141 ttttgttggt caatgtcttg cagatacatg tgtttatctg taacacctct tcactatagc
    13201 gccaaaaatg agaatttttt ttttataaca tcaaatccac gtagattttt acaaacaaaa
    13261 ataaataaag tcggattttt ctgataattc aacgcaaaaa agcgaacaat ttttcaataa
    13321 atcagtcttc taaaatagat aacctaaaaa aagcgaacaa acaaataaaa tgactgtcct
    13381 tcattcacta aacatatttt tattttttta tattgttaaa aattcgcaat ttatttttta
    13441 atagaattat ctgttttcca aattttataa cataaaaaac cttttttaac ggcatttata
    13501 tgctttttca gaaattaact gatgatttca aaaagaaaac atgtcataat atttcccata
    13561 ttgtgcaaaa aaatacaagg cggaggctct actatgattg aagaatatat tgagaaaaat
    13621 attttaagac agctgttttt atgtgggcaa ttctatgtaa ataaagaagt caatctagaa
    13681 aagctctcca atctcctgca tgtttgcaaa accactttac tcaatgatat caataacata
    13741 aaaaaagaat ttgaagaaca gatcgcttat acacatcggg aaaaggaccg ttacacttta
    13801 tatttttcag aacatatacc tcgatgcaag atcatgcagc aattgtctca aaattcgttg
    13861 tttttaaaaa cttgtctgct ttatttggaa gaagatgaac ctgattatct tcaattaact
    13921 gaatgtgaat tcatctctgt cagcaaagct tattcattaa aaaaacaagt attagcttat
    13981 tttaatgact gcgggatcga aatcgaccgc tactcccctc gattcactga aatggaaaga
    14041 cgtctgctgc ttcttaatgt gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct
    14101 gaatcgtttt ttgaacgtgc cgatcgtttc attgaatccg taacggaaaa tagtggcaga
    14161 ttttatgata aagaaaataa agaaatctta tcgattggtt ttgctatcag tttcctacgc
    14221 cagcaagtgt gtgctgtgac aatagattca aaatttatcg aagaaatcaa aaaacgtcct
    14281 atttacaatt atgtcgaatc agcatgggaa aacactgatt tccaaaccta ttataaaaaa
    14341 gaagaattcg cctttatttt gacactattc aatctttgta attatggttt ccattcttat
    14401 cagttgattg cggaagactt tcaacagcta caccaagtgt ttattgacaa tactcccgaa
    14461 ataaaagaac tagttgctac ctttgaatct cacttcaatc aagaattatt cggaaaccaa
    14521 ccgttcgaac gtgcgctgat tcatctgatg cgttcggctt gggataacta tcaattattc
    14581 atgccagaaa aattttatct tttaaatgag gaacagacaa atttatataa agaagtccag
    14641 acaatttttt ccagctggtc cagccagtta ccttatgatc tacggttgaa tcctaattgc
    14701 atgcgggctt ttgtgatcga attgagcgga attctccgtc tgacaaaaga acatctgact
    14761 atctatatcg tcacaaattc ggatgttcat tatctgattt atcgggaagc cttggaagct
    14821 gtaactactt tcgattttca agttgcacca acaatttatt caagtatatc tgatataaag
    14881 aagtatgcgc agcagtcctc aaaccgggta ttatgtgaac gtacattata tacaccagat
    14941 gcggtccaat atgaaaatat tattcctatt tcgatcaata cgatcgatcg agctatcatt
    15001 tcagctgtgc aaaacaaata aggggctgtg acaaaaatca tgtcacagcc tcttatttgt
    15061 tgaagatgcc catttctgta acaatctctt gatacacgtc aggctcacaa cctcacttat
    15121 tttggacatc tacgggtccg atctctttat ttaaaaaata agaaatgatg gttcggataa
    15181 ctaccaacgc cgctagttgg aaaatatcct gcaaggtagg tttgataatc gattcgatga
    15241 tcccagctga aacaagaatt tccaaactta gaagtatata gcctcccaac atctttttaa
    15301 tagcattgtt ctcgctgact cggtcgcttt gcacttttgt atggacaaat aaatatttca
    15361 aaaaatcctt gattgataga caaacacccc aaaccaataa acagatagca aagatatcaa
    15421 gtcccagtac aagtttttcg aaatatggaa gaagaaactg catcaactct gtcgtcatcc
    15481 tacctctcct cttctttgtt tttctttgtc ttctatttcc tgattcaaga aataagaaat
    15541 gactgttcga atgataataa taagtgcaag cttcaaaata tctgtccaag tcggcttgat
    15601 aactgactcg atgatatcag caacgactag tacttccaag cttaacaaaa tataacttgc
    15661 tagcagtttt cttgtatctg cattacgagt attacgttct gcatagtttt tcttgacatg
    15721 agaccaatca aaaagtttga atagaacaag aacgatcccg acaattaaga taccgataga
    15781 acaaatattt aaaatagcaa tgatccattc tagaaattca attaaatttg agcgaaacat
    15841 atacgctacc tactttcttt taatttgttc ctattctttt attgtaaaag aaagttacct
    15901 tgttcgtcta tttaaaagtt gttctaatga taacggttcg cttaaatttt tctttttcac
    15961 tctcccattt ttctccctcc atccataaat cgtggaagta tccaaaacaa cgtagcaagt
    16021 taaaataagg cttagtccgt catcagcttt tcaaagaagc actgtctcgg tgcttttttt
    16081 gtacccaaat ttctaaaaat agaaccatac acctcctcaa atcttttctt cttacctacc
    16141 ctatttttat ttacaggaaa atcagacact tcttcttttt tgtcaaaatt tcctttttct
    16201 tctcgtccta tcatttggca ttctaataaa aacgaactat ccttggatat agaaagagaa
    16261 aacaaggagg cgtttttcat gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg
    16321 cttgcccttg gcgctgcata tctaggaaaa aagacaggac tattcgaaga tgacagtcat
    16381 ttatatgatg aattcgaatc aatctagcat cctatttagt tgaatcacta cggacatact
    16441 tggaagacat tggaggaatc acaatgagaa aaagtaaagc aataatgatt ggtgccggta
    16501 ttgccaatat ggcagcggct gtttatttga ttcaagaagg caaatggaaa ggggatcaga
    16561 tcacctttta ttcattggat gatcacggtt caaatgatgg tgcaccgacc gacactgtag
    16621 tggatgaata ttggaataaa aaccatccat tagaaaacca aaaaggctat attgctcgag
    16681 gcggtcgcat gcttaattac cgtacctatg tagatttgat ggatctgtta agtcgaatac
    16741 cttctgttac tgagccaggg atgactgctg aagaagatac gagagatttt gatgcaaaac
    16801 accgaacctt tgacaaagca cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa
    16861 aactgggatt caataataaa gaccgtattc ttctgactaa actgattgct atgcctgatt
    16921 cagaagaaga aaaactagac aatatcacta tcgaagaata tttcaaagat gatccacact
    16981 tttttgaaac aaacttctgg tttatgtggg aaacaacatt tgctttcaga acacgtagtt
    17041 ctgctcaaga attgcgtcgc tatatgcatc aaatgattta tgaattcact caaatcgaac
    17101 atctagtggg cgtcaatcga acacgttaca atcaatacga aagtattatg cttcctttga
    17161 tcgagtactt gaaagaacaa ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg
    17221 aattcaaaga aacaccgatg caagatgaaa tcactgtgac tggtttgaaa attaccaata
    17281 cactgactca agaagaagaa tacgttgtgg tcgatgaaga tacagcagtt attttcacta
    17341 atgggtccat tacagattca gctactttag gagattacga aacaccagct gtagaaaata
    17401 tggattatgg tgcagcttct agtctctgga aaaaagccag tgaacgtttc tacaacttgg
    17461 gaaatcctga caaattcttt gctgatcgag atgcaagcga atgggtcagc tttaccctga
    17521 cgacgaaaaa ccatttgttg ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa
    17581 atgccttgaa ttcatttatt tcaaccacac cgatcacgcc gttaggccaa aaagatgtca
    17641 atatgtctat tgtcgtgcat catcaaccgc attttacgtc tcagaaacca aatgaaacag
    17701 ttatttgggg ttatttcctt tatccaagac gacgtggcga atttgtggat aaacagtata
    17761 ttaaaatgaa tggaaaagaa atgctagaag aactgattgg acaactttca aaagtagatc
    17821 ctggaccagt gaatattcgt gaaaaagaaa cagaaatttt cgacagcgta ataaataata
    17881 tccctgtcta tatgccttat gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac
    17941 caaaagtcat tcctaaacat tcgaccaatc tggcctttac aggagaattt gtcgagcagc
    18001 cttatcagat gatctttact gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc
    18061 attttgcggg tattcctatg tcccgtcttg tcaaaacacc gcgttacgac aaagatattc
    18121 ctactctagc tcgtgccgca aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg
    18181 ccctgcatca agtaataaga acaaccaggc taaaacagct tcttatgttt ttgatttggt
    18241 tggacttatt accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag
    18301 ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt
    18361 ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa
    18421 aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc
    18481 ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg
    18541 tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc
    18601 atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct
    18661 ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt
    18721 atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa
    18781 ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa
    18841 aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg
    18901 tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt
    18961 tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc
    19021 tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg
    19081 tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca
    19141 gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc
    19201 ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct
    19261 tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga
    19321 aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct
    19381 attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt
    19441 acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga
    19501 cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat
    19561 cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag
    19621 cttacaaagc ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg
    19681 aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt
    19741 ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa
    19801 aatttacgca atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct
    19861 gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat
    19921 aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa
    19981 aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg
    20041 acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt
    20101 attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg
    20161 atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag
    20221 aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa
    20281 aaaagaggca tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt
    20341 tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg
    20401 tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga
    20461 tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc
    20521 ggttcaacaa acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt
    20581 tcaatgacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga
    20641 ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga
    20701 actccccatt ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt
    20761 aatttgatcc agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca
    20821 aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa
    20881 acggaaaaca atcacttgtt ctccgttttt tgattaatca agcatctaaa gcttcttttc
    20941 ttctttcttg gatcgtagct accttgacga acggtagata gatcaaaata gaaagaacca
    21001 aattgacagc ggctagtaca ccacctgcaa tactttttgt tgccaataca ccgccaataa
    21061 ttggtggagt tacccatgga ggcataacgg tagctgctgg cactaaacct gtagaagtcg
    21121 caaagtaagc aacactaact aagaccattg gtgtcaaaat aaatggaatg aacatgatcg
    21181 ggttcaacac gataggtaac ccaaacatcg ttggttcatt aatattgaag acacctggcg
    21241 caatcgacaa gtttgttaca accatatatg gtttgttttt acgtcctact aaataaatag
    21301 ctaacagtaa acctaaagtt gcacctgttc ctcctaagtt gacaaagctg tcaaagaaag
    21361 gtttgttcac gataaacggt gcgctatgtc cagctgcgat tgcattaacg tttgcttcaa
    21421 tggctggcgc attgattgtt tgcatcaaag gatcgaccat attcgctccg tgtaaaccaa
    21481 agaaccataa gaatggtgtg ataaatgcaa gcagtaaggc agatggataa gagtttgcaa
    21541 gtcccataaa tggttcttgg accactgtat agaaagaacc tacgatatca tcaacaccga
    21601 atgcagcaaa aattgcagca actaaagcaa acgcagagat agtgatcatt gctggtagta
    21661 atgaagcaaa tgcttttgca actgccggtg gcacaccatc aggcattttg atgatcaatt
    21721 tttcatttcc gcttaaacgt acaaagattt ctgttgaaat aatcccaaca ataatagcaa
    21781 taaacaaacc gtttgagctc atacctaatg ctccgcctaa agcgaagaaa gagcctaaag
    21841 aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt agctaaatga taagcaagca
    21901 agaaagcaat caagacagaa agaatggcaa atgtaccgtt ccaagcagca gccccgaatc
    21961 ctttccatga ttcaccacca aaaattgagt tcatcaattc ttggaacccg ggaatcggta
    22021 agttattgat cattactgct aatgctccta agatcattaa cggcatactc accataaatg
    22081 agtcccgaat cgcaactaaa tgccgttgtg caccaatttt tgatgctaca ggtataaaat
    22141 gtttctccat aaatgcaaca aatttatcca taaatcttcc ctcttttctt tactgtagat
    22201 tacgcgttca atcgacgata cagatcaatg atctcttttg ctaaatctgt aaaagctata
    22261 gatgtcatca gatgatcttg actgtggacc gtcaaaagcg tgacttcaac gtgatttcct
    22321 tgtgcttctt gtgtcagcat ctctgtttga gagtgatgtg cttggactaa agattcctct
    22381 gcatctttga ttttttcatc tgctaaagtg aaatcacctt ttttcgcagc agcaatcgct
    22441 tccattgcat cgctttttgc atttccgcca aacatgatca gacccatgat ggcttctaga
    22501 tttttttgat cttccattta ttgtatcctc cgataatttt tattatttgt ttattagttt
    22561 ttctgcttgg tcgagtactt tttcaccatt catcatgcca tagtcagcca tattgattac
    22621 atctaaagga atgcctttag gtgctagctt ttcagcaaat tgattttgca taaagcgtac
    22681 ttgcggtcct aaaagtaaaa cgtctacatt tttagattct agttgattat ccgcatctga
    22741 agcagataca gcaaagatgt ctgcttccat tccttttgct tctgctgcct tttgcatttt
    22801 tgtgactagg agacttgtac tcattccagc tgaacataca agcatgattg ttttcttttc
    22861 cattttaaac acgttccttt ctatacttac acttgtatat aagcaagtag cgtgccaact
    22921 ttaaaagcgc tttaaaaagt tgatatcatc acatttaagg tctataccaa gttgcataca
    22981 cactaaaaaa gaaagcgcac actaaacaca caaattaatt tttattacct tttttctttt
    23041 aacgtgcaca aaaaaagttg tgacacaaaa cttgtcacaa cttttttcaa ataaacggta
    23101 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacataa gatgttgttt
    23161 ttactttgct attcttatta cttgatgcag gacagctttt tcacaacctc tcaataaacg
    23221 gtgttcaatc agctgaccct gggtattagc tcaaaacgct ggattcacaa cctcttttat
    23281 ttcaaataac cagtaaatac aatgaacatc tacaaaaaac ttctatttat ttatagattt
    23341 cgtcaggcaa atccaattct ctcttatcaa tcaaagtctg ataccacgaa gcagattttt
    23401 tcaatgttct gcttcgttca tcgttcaatt cgattcgaac taatccatat cgattcttaa
    23461 atgcattcat tggtgacaca cagtcagtga atgcccataa catatagcct tcgcaatttg
    23521 cgccttcttc tgttgctttg atcaagtaat gcaaatgctg tccgataaat tcgatacggt
    23581 aatcatcttg cacgattcct ttctcatcca tatactgttc ttcatattct cttcccatac
    23641 cgttttctgt tatcagccaa gggatattat gataattttc ttttagatac atggctatat
    23701 catacatgat tttcggatag atttcccatc ccctggaatt attcattttc ttcccaggta
    23761 aatcaaagac ttcataatat cgttcaggat ggaaaggtgt ctcttcattc cattgataac
    23821 gaggggcgcg aacgcgttta ggataatatt gattgattcc aaggaaatct actgtatgat
    23881 gctgaatcgt ttctaaatcc tctgttgcag gatcgaaata aatcgattgt ttttgacaca
    23941 actccaggaa ctcttctgtg tagtatcctt ttaccatcgg atcaaaaaat actcgattat
    24001 aaaacaaatc atacatataa gcagcttttt ggtcttctgg agaattggat cgagcataga
    24061 ccatttcagg atttaaaata cagccaatcc ttccaccata atttccgctg cggtaaatct
    24121 ctactgcttt agccgttgct aaaactttat gataattcca catcatccac ttttttgtgt
    24181 tttgttcatg cggccagcga acagcatcca aatagcaacg cgtttgggga acgattggtt
    24241 cgttgaatgt aaaccagttt tttaccctat caccgtaacg atcaaaggca atttgagcat
    24301 acttagcata aaaatcaact acttttcttg aagaccagcc atcatatttt tccaaaagaa
    24361 aagctgggac ttcataatgt tccaaacaaa tcatcggttc tacgtctgct tcgatcaaag
    24421 agtcgatcat ttcacttata tgctgagcat attcttcatc tactatacag ttctcataat
    24481 cagtaaaaaa acgagcccaa ttgatagaag tccgataatg tgtcagatgg atggatttca
    24541 tcagatccac atcctgctgg tattgttcca taaaattagt tgctacggct gggccatacc
    24601 cattatgcca aacaaaatgt tcatttttat accagctatc tataaacgaa tcctgtcctt
    24661 ctttttttcc tttccaacct tccgtttgcc aagcagaaga tgcagcacca agaataaaat
    24721 tttttggaat agtaattttc atgatttcct cctgtttttt agatcccatc attttccaca
    24781 cgatttgcta cttttacaaa tggcagatag atcaaaatag aaacaataat acaaacaagt
    24841 tgcgtaataa ctgcaccgat atttcctgct gttgccagat aagcattgac gatcggcggc
    24901 attggccaag ggaccattac aactgctttc gccgcaaatc ctaaagcggt tgcaaaataa
    24961 ccaatcgttc cagtgataag aggcgttaag ataaacggaa tagctaaaat tggattcagc
    25021 atgatcggca taccaaaaat aactggttca ttgatgttga agatcccagg agcaagggat
    25081 aacttggtga tttgtttcat atcttcccgt tttccaacta acaatactgc aatcagtaaa
    25141 ccgatcgtga ctccagaccc gcccatactc atatacatat cccaaaatgg catagtaata
    25201 atgttcggta cttcttttcc agcttcaaaa gcttccgtat ttacagcaat tgccgctaaa
    25261 agcaacggtt cccgtatcgg cttcaccatt tgatttccat ggataccaat cacccaaaag
    25321 acttgcgcaa taaacatcaa tagcaaaata cccggtaacc cttgtacgac accttctaat
    25381 gggcgttgta cgacattgta aatgatatca taaaggtaca ttcctgtaag ttctttgatg
    25441 ataaatcccg cagccgcgat acccgataca gtcagaatcg tcgggaataa agcagaaaaa
    25501 ctagcagaaa cattcgttgg cacggtatca ggcattctca gctgtagttt cttctgtttc
    25561 ctcaaccagc aataaagttc aatagaaagg atggagacca acattcccaa aaataaccct
    25621 ttcgtatctg tatattgttt agccaatacg ttttctacta taacagtctg attttcattc
    25681 actaattcat aaacagttgg attaacagat aaataagaca tcactgcgat gataccaggg
    25741 aatacaccat ccactttatt cagcttggct aattcgatcc ctatcagaaa aacagcatag
    25801 atggtcaaga aacttagtgt gacatagctg atggcagatg cgataggctt taattcttct
    25861 aaaaaacgca agaaacttat ctgtgctaag ccattttccg agtcaaagac cattgctgaa
    25921 aacaatgtgc caaaagcacc cgtaataatt accggaatca atgcagcaaa tgcgtttttt
    25981 atcaccatga tataccttaa attattgatt ctcgttgcca cgttccctaa aacattggct
    26041 aatttatcca taaaactcat tccagatccc ctcattcctg cttcttctcc ttgacgtcat
    26101 ctctttttgt ctattagtca gcgcttacat ttcgatgaac agaaactatt tactcctctt
    26161 ttttaccaaa tataaaacaa aaattcatct gatgtattta tcataaaata catcagatga
    26221 actgtcaatt agtttctttt tctcctatta ttttctgtaa ttctctccga tttattgcca
    26281 tatggacagt cattgcatca aacgcagcaa gtgcattccg cctttcgatg gcctgaacga
    26341 tctctgtatg aatatcgatc gtctcgattt tcagatgctg gatatcataa tttttattaa
    26401 acaaagcaat tgattcatta atgattggga taatatgcag caaggctacg tttccgctag
    26461 cttctgcaat catcgtatga aaatcgatgt ctaattgaac atgttcttgt gtgccttctc
    26521 gaaaagaagt ttcaatcttt tgcgttaatt ttttcatttg gacgatttga ttcttcgttg
    26581 catattccgc tgccaaacct gccatcttag gctccaataa atatctcagt tcaaataagt
    26641 ctcgtatcat ccgatcctga tcttcaatca aagaaaaacc gagtgggtca ttggcaatcc
    26701 ctcgcttatc actgacaaat gtccccgctc cttgccgaac ttcaaggata ttcctggaag
    26761 ctagagcccg cacagcttct cgcaccgtgc ttcgccccac ctctaaataa tcggacaatt
    26821 catattcatt cggcagttta tctcctactt gcaactgcat ttcctttaaa tatctaagga
    26881 tcttgtcagt agttacttct actagggttt tattttcttc caaaccacat acctcctttt
    26941 catctgatgt attttttcgt aattattata acacgtttga atcatctgat gattttttgt
    27001 atacaaaatt gattaatccg ttcatagaca tacaaacgaa gaagatattt accataaaaa
    27061 agactaggat aaaattcact cctagccttc gatcattttt attttgaacg acaaaaaata
    27121 tgatgttcaa taacagacga tcttcattcg attcttgatg tcatcttttg ttttttttaa
    27181 tatatggata aaaagtttga ttacatcgtt tctattctag aatttggtcg attctcaatt
    27241 cttcttctgg tgagaaatct aaattatcca tcattcgaac actttcttgt aactgactta
    27301 accgacttgc tccgaccagt acacttgtaa cagatttttg tcgcaaattc catgcaaggg
    27361 ccatttgcgc taatgattgt cctcgtgata cggcgattgt ttgcagtgct ttgatttttt
    27421 ccagagttga tccaacttgt tcttctgaaa ggaatggtat ttccttacga tgtgctctag
    27481 aatcttcagg aataccatgt agataacgat ttgtcagcaa cccttgagca agcggactga
    27541 acgtgattgc ccctagccct tcttcttcta atactggaga tagatcgtct tctagtagtg
    27601 cacgagaaaa catattgtag cgcatctgat gaataatgaa cggtgcttcc tttcttttta
    27661 aaatttcagt catttttttt gtatcttctc cattatagtt ggatatacca atatataatg
    27721 cttttccttg tcttacaagt tgcatcaaag cttctgctgt ttcttccaaa ggtgtatcag
    27781 ggtctgggcg atgatgatag aagatgtcaa cataatctag ccccattctt tggagacttt
    27841 ggtcacaact tgaaatgatg gatttacgag agccccattc cccatatggc ccttcccaca
    27901 tatgataacc tgctttgcta gaaataatca gttcatcacg gtaggcttga aaatcttctc
    27961 taaaaatacg cccaaagttt ttctctgcag ctccagcagg gcctccataa ttattcgcaa
    28021 ggtcgaaatg ggtaattccc atgtcgaatg cgcctcttaa aatctctctt tgattatgga
    28081 taggatcata atcaccaaaa ttttgccata atccgagaga taaaatcggt aatttcaaac
    28141 ctgaatgacc ggatttacga tattccattt tttcataacg tgcactatct gcaatataca
    28201 atttttctcc tcctcatcaa atctttttaa aacgctttct tttttctatt tttagtttac
    28261 tacattttaa agaaaaggag ttaatcaaaa tgattttttc gtatattttt ttgccatttg
    28321 ttttttactc gttgatagat cagaaattga ttacgtgctt gctggatttt tttataacgt
    28381 ttcggttgtt ttttgtaaaa ttgctgatga tagttttctg ctggccaaaa cgtaccggct
    28441 ggttcgattt ttgtgacgat tggttggtgg aaagttcctg aactgtctag tttctgtttt
    28501 gattgttccg ctaattcttt ttgtcgttcg acttgataaa aaatcactgg acgatattgc
    28561 ttccctctat cttgaaattg tccaaatgta tctgtgggat cagtaatttg ccaatagatt
    28621 gtcagcaact cgctataact tatttctctt gtgtcataaa tgatctccac agcttccaca
    28681 tgacctgtgt aaccaccact tacttgatca taatttggtt tttcaacatg tccgcccgtg
    28741 tatcctgaca aaacagatag gatccctttt ttcgattcaa acggctcgac catacaccag
    28801 aaacatcccc ctgcaaatac tgcacgttct tgatagtcag catcttgcat ggcatgcttg
    28861 gaaaagtcat atttttgctc cttatcaaat ccttccgtaa tcaataaata aaagtccatt
    28921 acatcaggcg tcagattcat tcggatagct aatggacgta gtgttgcttc tagttttgat
    28981 aactgttctt taacggatat attttctctt atgtcattct ttgtctgaat caatactttt
    29041 cgttcccaat ctcgtgtatt cggattcaaa atcagattat aaagtgtttg aatgaatatt
    29101 tgttcctcat ccatagattc tcctcctttc cgaagaagac aagctagtga ctttagctgt
    29161 aatttatcat aaagtgagga aataagcgat tacttcatcc ccctacagat ttttcgtata
    29221 aaaaagagag aatccttatc gacagattct cctttttaat caatttattt tataaatgaa
    29281 aagttaattt accttttatg gaaaactatt ttaaaaaata aacataaata tgcagttaat
    29341 gtaacaagat aagaaataat agtcgttact gtataacttg tgcttacttt aatatcagca
    29401 actcctttga ctccgcttat ttgtaccaat ccattagcat tcacctgctc ctttacaggt
    29461 tgatgattta tttctacatt cgttcctaaa taagcatata caggaagtgt gattacttgg
    29521 ctcttctcat tagttactct atatatgata gaactaccag ttgttttata aggaaggttt
    29581 acccattgac tgccaatctt tgcttcgtgt tttattatat gctccttatt ctccaatgaa
    29641 gcgataggag aataatcata taaaaaataa tccattcctt tagtggtata cgcctctact
    29701 gtttctttgt ttacaacaat atgtcccggc tgagaaatta ttttttgatt gaaatttttt
    29761 ttactagaaa cagttaaagt gattaataac aaaacagtca taaataaata aaatagttct
    29821 cttgtcttat tgtatcttct atttttgaaa aatatcatcg ttaatatcag agaagagaat
    29881 attatttgca agcttaagat ccgccaagga aattgtaaca atttggctgg cgtattttgc
    29941 agaagatacc atggcaccaa actagttgta gctagccaag tcaaacacgt accataataa
    30001 atcttttttt ctatgtttgt taatttttta aataaaatca ggggaataca taaagataac
    30061 agaccaacta gtcccattga gtaagtagtt aagtcgttgt tcatagcttc agtcagacta
    30121 tcgaacacat tagatgcatg agcatataaa tttgtcacgc taggtttgtt gattttttgg
    30181 tagagcattt gttcaaacat cggataccaa aaagcactcg tcagaaccaa tgtcattcct
    30241 gccgcactaa ataatttttt aaatatagca ataaaatctg cattcttgaa acctttagaa
    30301 accaaacgaa taaaaataaa aatgataatt aatgctgtac tcattaatgt tgatagtaca
    30361 tggctgtaca ctagcaaact catactcaat gctagcttta cagacggata cttttcttct
    30421 tttccagaga tgatttgata agcataaaat aatacaggga taacaagtgt tattgcgata
    30481 gcttcggcaa ttgcggaacg atagtagatg tctactgtcc gataagcaga gaaaatatag
    30541 agacaactaa ataaaaatgc cgcttttttt tgttttaaaa accgctcgcc ataatgataa
    30601 gctacgagtg ttgtacacaa gttcaataac caaacgtaaa taatatagct tacaatcaaa
    30661 ttttttgtca tccaatataa cattacagct ggaaaaaaag tcagaaaggg ataaaaataa
    30721 tttacaccat atccagtata attgaatgtc tcataattaa tcggactggt aaaaatagtc
    30781 gataacccct tgattcgaga taagtgaaaa gaaagatctc cagtggtata aatactctgt
    30841 gtatttttga aaaagatgaa agaatatata ctactaacta taattaatcc actcaatatt
    30901 actacttttt tatttatttt cattcttctt gtttatctcc tttttagtca catatcgaga
    30961 atctttcaac ttattaggtg caaatcgttt aaaacttgaa tacaccacta aacaataagt
    31021 cagaatactg atccataaag ccatgagagc agttggagta tattttgttg taatttctat
    31081 cttattttct ccagcattta catccacatt cactaagcca tgttttttat aagagtcatt
    31141 tacgacttta ccgtttactt ttacctgtgt acctaaataa gcatatactg gtactgtcac
    31201 tttttgcttt ttgttgcttg ccactgtgta agtaatgtta ttatctgcag ctttatactt
    31261 ccctttttcc cacgattctc ctacttttac ttcatgtttt tctaagtgag gcctttcttt
    31321 tatcgcttct gttggggcgt aatcgtataa tcctcccata tctgatgttg tataacgtgg
    31381 gagtgtttct tcattgataa caatatgacc gtgttttgat tggataactt ttgcgtaatt
    31441 ttcttttgct gctatagacg tgacgagtag aaacaccaca gtagcgccaa tgattaacca
    31501 tgaatatttt ttattcatag tctttttcat aaaaacaagc gttatgatca ttgaagaaaa
    31561 aaagatttgg atacctaaga tacgccaagg aaactggagt aagcttatcg gtgttttttg
    31621 aaacaaaaac catggaaata aattagttac gcatacccat gtagaaacta cgccaacata
    31681 gatgattttt tcttttttct ccatttcttt cagtatgaat aaaggaaaaa tcaacgcaac
    31741 caatcctaat aaccccatag tgtatgttgt aatatcgtta tttaacgctt gaatgatact
    31801 atctgaaata gaaagtgctt ctagctgcaa atctgttttg aagggactgt gtatttcttg
    31861 ataattcatc tgctgcaaca taggatacca aaaatagcta gttaatgcac aagtcaaaaa
    31921 aacagccttt actaaattca cgaccacaaa ttttatatcg ttttttattt gcttgcttaa
    31981 aaacaaacga actataatca tacaagcaat caatgcacta cacaatagtg tagttaagac
    32041 atgagtatac acaagtagac ttatactgac agataaagcg atgacttttt tttctctatt
    32101 gaatatcaat ccataagcat aatataaaac agctgggata atcgtaagag cgattgcttc
    32161 tgcaattgcg gatcgataat agatatcaac tgttcgataa gttgcaaaag tatataaaca
    32221 actaaataaa aaagcagcat ctatcttttt aaaaaacttt tctccataat aaaaactaat
    32281 cagaatcgta caaatattta ataaccaaat atatagaaca taacttaata ttaagttatt
    32341 tgaaattcca taaaaaacga ctgcaggaat tattgtaaga aaaggataaa aatagttcag
    32401 tccatctcca taattgttaa atgttgtata gttgattggc ccttcaaaaa tagaagataa
    32461 ccctttgatt cgtgaaagat gaaaagataa atcacctgtt gtataaattt cttgagcatt
    32521 tctcataaaa tttactgaat ataaagtact aagtaagata agaactacta aaagaaatgc
    32581 ttttttactt attttcatag atttgttgtt ctcctttttt aattctattt tcagtatact
    32641 agaattgtct ttttttaata agttttgcgt taaatttttc atagcactag tatgatatac
    32701 tagaaaagaa tcaattttta ttacttttaa ataggagaaa aaaatgaaaa aaaacaaaaa
    32761 ccaaataata gatatcattt ttatgttatt tctaagtttt gcttacgtca tccctctatt
    32821 catgtctaag ggaatctatc atagtgtcaa tcaggatact tactttcatt tatctcgaat
    32881 cataggacta gataatgtat ggagttctcc agtaaacttc aataacttcg atcatcatgg
    32941 aacaatgatg aatatttttt atccatggct tactctttat ccagcttttt tattttataa
    33001 aatgatggga aatcttgttt taagttataa tatctactat ttttttatca cttttttaac
    33061 aatggtcgtt tcttattttt ctatgaaaca aataaaaaac aatcgttata tttcgctgtt
    33121 attttcgatt atttatacat tttccgccta tcgtgccatt gacatattcc atcgtgcttc
    33181 attgggagaa gctgtcgctt taacattttt gcctttgata ctaatggggt gttacgaaat
    33241 ttatattaga gattaccaaa aatggtactg gttgtctatt ggcatgacgc tagtagtgta
    33301 tactcattta ctttctgtcg ctatggtttc tgtatttatt ggaggaacgt tgtttctttc
    33361 tttttatttt tgggaccaaa aaatcgctag actcttaagt cttttaaaag ctaccgtcct
    33421 cacctttttt ttaagtgcgg gattcttaat tccttttatt caacaatcta gagcacaaga
    33481 attgaaagtc ccactgggaa aagaattaag tggtatggct cctagtgata tgctgacaca
    33541 tattttaaat aataactata acaactatac tattggatta ttcttatttc tcggattgat
    33601 tggtgctttt atttttataa aaaaattaac tgctgatgat ttatttattt tctttcttgg
    33661 tgtatttgtt cttttttgtt caaccaattt atttccttgg caacttttta accatacacc
    33721 agttaagagt ctacaatttg tctggcggtt aaatggtttt tcttctttat ttatagccta
    33781 tacgatgagt atagtgatct attatatttt ttctactaaa aataattcat ggaaaataat
    33841 ggtctttacg tttcttgcac taatattgca tctctcagga gtaagcaatt ctatccatgc
    33901 taacaaagat tcattaacac ttatagcacc tgaagacgct gttgctatag ctgaaaatta
    33961 caatcatact gattatgcaa acaaagagtc aatctatcat cctgatatga ttaataatga
    34021 tcaatattta ctaggtaacc aggaaataaa tccaacaact cattttatga gtaacaaact
    34081 tacaattgaa ttagataatt cgaataataa aaatactgtc ttgacgacac ctatttatcg
    34141 ttataaaggg caagtggctt ctattaatgg caaactagta caaacaaaat tatctaagtt
    34201 tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta cttatcagta
    34261 cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt tatttttact
    34321 ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac attctcacta
    34381 gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa agtctttttt
    34441 tatatttctt taatttgctt catacattat ttaacatcga tatttatact gtgaaacatg
    34501 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
    34561 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
    34621 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
    34681 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
    34741 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
    34801 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
    34861 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
    34921 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
    34981 tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
    35041 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
    35101 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
    35161 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
    35221 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
    35281 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
    35341 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
    35401 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
    35461 tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa aaaactgatt
    35521 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
    35581 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
    35641 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
    35701 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
    35761 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
    35821 atggctgctg taactagggt agctaacaca cgagatgtac taacaaatgc agcatacaat
    35881 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
    35941 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
    36001 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
    36061 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
    36121 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
    36181 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
    36241 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
    36301 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
    36361 ccattgaatc ctggataaca ccgataataa atccaatacc gacgatttgc atcgcgacat
    36421 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
    36481 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
    36541 aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
    36601 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
    36661 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
    36721 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
    36781 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
    36841 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
    36901 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
    36961 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
    37021 gaaaagctag tcccaacaat acagcccaga ataagacgcc aaggtaattc gcatcaataa
    37081 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
    37141 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
    37201 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
    37261 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
    37321 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
    37381 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
    37441 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
    37501 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
    37561 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
    37621 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
    37681 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
    37741 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
    37801 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
    37861 caaacttccg ttgttattgg tgcaaatttg atcccacttt acatgggtta ccaatatcaa
    37921 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaata
    37981 gccacactag tttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
    38041 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttcttgtact
    38101 ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc gaaaacaaca
    38161 attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac tctatttaat
    38221 aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta tttattttta
    38281 atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa gcttcctcaa
    38341 gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca aatttcactc
    38401 atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc tctcttcgca
    38461 atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa attaggtata
    38521 aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat atgtaatttc
    38581 cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa ctcagggaag
    38641 gcatggctga taaatatcgg gcagtttttt atttgctatt tattagcagt gtccctccta
    38701 gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat cgaatacttc
    38761 gcttttgatt cgattgaaca attaactcag cgttggaaaa aaagaattca ttggcttttt
    38821 gttcataaaa gttatttttt agttgcagca tttttttact gctttacctt cgtgcagatt
    38881 ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt aaaaatattt
    38941 actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt tttcttctta
    39001 ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat cattaactta
    39061 ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt ctttccttca
    39121 gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc agtcttatta
    39181 atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt acaacgtaga
    39241 ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag tatcgctgta
    39301 ttattaggat gtttttcggg tgtctttttc attaatcaca aaaattcgcc ttctttcttg
    39361 ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc cgtaaaagat
    39421 aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga agaacctgct
    39481 ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa ggcagatgaa
    39541 atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa tttaagtgaa
    39601 agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc tattccttac
    39661 atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg ttatggtggc
    39721 ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa cctgtctgct
    39781 tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc aaatattacc
    39841 gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta tcgcagaaaa
    39901 caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc ggataaatta
    39961 acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc atacaaagaa
    40021 accttgaaag ctttacaaag caatacggaa acgtcacaat tcatccaatt atccactatg
    40081 caaaaccata tgccttatga tgattactat agtgaattga actacactgc tgaaggaact
    40141 gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct tcactatacc
    40201 gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc gattaccttt
    40261 gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa aaaatatgga
    40321 ttagaacagc accagacaga ttatttcatt tataacaaca agtatagtcg tgaacaagca
    40381 gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc tcttgagcat
    40441 gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa tcatctacct
    40501 gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa acaggtcttt
    40561 gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca aaaagaaatt
    40621 ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta cagtgcaaca
    40681 tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg tgataaaaga
    40741 catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat ttgattaaag
    40801 ccatttttca attcattatt cgctgtctcg ttgaagtaaa gataattatc gggattcccc
    40861 tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa tagatagacg
    40921 tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat ttgatagtct
    40981 gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc aatagccatc
    41041 ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc cacactcatt
    41101 tgattcgtat aacttttgta ttgttgtccc attgtttggc cccatttggt cataaatgcc
    41161 tctaattgtg cggatttgtc tgcattccac agtgtttgtt caacttgttc gctgctttct
    41221 gaagaggaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
    41281 gttttggaag actctgacga agtagaggta tctgctgttt ctgtacttgc tgcagttgac
    41341 ttgctctctg tgctagttgt tgtagtagtt gcaactgttg ctttcgctgt ttcttttgtt
    41401 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
    41461 gttactaaaa tccttttttt
//
LOCUS       DANKHJ010000017        41001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000017 DANKHJ010000000
VERSION     DANKHJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(37..1929)
                     /locus_tag="PW113_001048"
     CDS             complement(37..1929)
                     /locus_tag="PW113_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525376.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1926..3653)
                     /locus_tag="PW113_001049"
     CDS             complement(1926..3653)
                     /locus_tag="PW113_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525377.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3669..4319)
                     /locus_tag="PW113_001050"
     CDS             complement(3669..4319)
                     /locus_tag="PW113_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2525378.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4523..4819
                     /locus_tag="PW113_001051"
     CDS             4523..4819
                     /locus_tag="PW113_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2525379.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5011..5631
                     /locus_tag="PW113_001052"
     CDS             5011..5631
                     /locus_tag="PW113_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2525380.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5723..6889)
                     /locus_tag="PW113_001053"
     CDS             complement(5723..6889)
                     /locus_tag="PW113_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2525381.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6901..7254)
                     /locus_tag="PW113_001054"
     CDS             complement(6901..7254)
                     /locus_tag="PW113_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2525382.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7382..8404
                     /locus_tag="PW113_001055"
     CDS             7382..8404
                     /locus_tag="PW113_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2525383.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8423..8584
                     /locus_tag="PW113_001056"
     CDS             8423..8584
                     /locus_tag="PW113_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525384.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8843..9277)
                     /locus_tag="PW113_001057"
     CDS             complement(8843..9277)
                     /locus_tag="PW113_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525385.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9387..11246)
                     /locus_tag="PW113_001058"
     CDS             complement(9387..11246)
                     /locus_tag="PW113_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2525386.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11364..12524)
                     /locus_tag="PW113_001059"
     CDS             complement(11364..12524)
                     /locus_tag="PW113_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL2525387.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12831..14255)
                     /gene="araA"
                     /locus_tag="PW113_001060"
     CDS             complement(12831..14255)
                     /gene="araA"
                     /locus_tag="PW113_001060"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2525388.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14271..14969)
                     /locus_tag="PW113_001061"
     CDS             complement(14271..14969)
                     /locus_tag="PW113_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2525389.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14926..16587)
                     /locus_tag="PW113_001062"
     CDS             complement(14926..16587)
                     /locus_tag="PW113_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2525390.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16805..18205
                     /locus_tag="PW113_001063"
     CDS             16805..18205
                     /locus_tag="PW113_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2525391.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18457..19536)
                     /locus_tag="PW113_001064"
     CDS             complement(18457..19536)
                     /locus_tag="PW113_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2525392.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19622..20581)
                     /locus_tag="PW113_001065"
     CDS             complement(19622..20581)
                     /locus_tag="PW113_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2525393.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20593..22104)
                     /locus_tag="PW113_001066"
     CDS             complement(20593..22104)
                     /locus_tag="PW113_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2525394.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22126..22689)
                     /locus_tag="PW113_001067"
     CDS             complement(22126..22689)
                     /locus_tag="PW113_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2525395.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22695..23558)
                     /locus_tag="PW113_001068"
     CDS             complement(22695..23558)
                     /locus_tag="PW113_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525396.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23572..24468)
                     /locus_tag="PW113_001069"
     CDS             complement(23572..24468)
                     /locus_tag="PW113_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525397.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24643..25932)
                     /locus_tag="PW113_001070"
     CDS             complement(24643..25932)
                     /locus_tag="PW113_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2525398.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25904..27421)
                     /locus_tag="PW113_001071"
     CDS             complement(25904..27421)
                     /locus_tag="PW113_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2525399.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27619..28509
                     /locus_tag="PW113_001072"
     CDS             27619..28509
                     /locus_tag="PW113_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2525400.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28582..29406)
                     /gene="nadE"
                     /locus_tag="PW113_001073"
     CDS             complement(28582..29406)
                     /gene="nadE"
                     /locus_tag="PW113_001073"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2525401.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29418..30890)
                     /locus_tag="PW113_001074"
     CDS             complement(29418..30890)
                     /locus_tag="PW113_001074"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2525402.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31527..31889)
                     /locus_tag="PW113_001075"
     CDS             complement(31527..31889)
                     /locus_tag="PW113_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2525403.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32112..32864
                     /locus_tag="PW113_001076"
     CDS             32112..32864
                     /locus_tag="PW113_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2525404.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33004..33927)
                     /locus_tag="PW113_001077"
     CDS             complement(33004..33927)
                     /locus_tag="PW113_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525405.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33942..34214)
                     /locus_tag="PW113_001078"
     CDS             complement(33942..34214)
                     /locus_tag="PW113_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525406.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34558..35745)
                     /locus_tag="PW113_001079"
     CDS             complement(34558..35745)
                     /locus_tag="PW113_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2525407.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            36109..37875
                     /locus_tag="PW113_001080"
     CDS             36109..37875
                     /locus_tag="PW113_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2525408.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            37977..39236
                     /locus_tag="PW113_001081"
     CDS             37977..39236
                     /locus_tag="PW113_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2525409.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            39383..40684
                     /locus_tag="PW113_001082"
     CDS             39383..40684
                     /locus_tag="PW113_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525410.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            40687..>41001
                     /locus_tag="PW113_001083"
     CDS             40687..>41001
                     /locus_tag="PW113_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525411.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYR"
BASE COUNT        12280 a         8364 c         7216 g        13141 t
ORIGIN      
        1 cacagcccct aaaaaaaata ataaatttgt actattctat gaagcttctt cttcagaaaa
       61 ctgactgtta taaagatctg catagaatcc gtctttttcc atcaatgtat cgtggtttcc
      121 tgtttctacg atcgaaccat gattcatcac aataatgttg tctgcatcgc gtattgtaga
      181 caagcggtga gcaacaacga aacttgtccg gttttccaat aggcggttca tcgctttttg
      241 aatcagtatt tctgttcgtg tatctacact agatgttgct tcatcaagga tcaggacatc
      301 tggatttgct aagaatgccc gcgcaattgt gattagctga cgctgccctt gcgaaatatt
      361 gcttgcatcc tcgtttaata cagtgttata tccttctggc aatttacgaa caaaatcgtc
      421 tacgtgagca gctttagcag cctcaatgat ttgttcgtct gttgcatcgt cgttcccata
      481 tttgatattg tcatagatac ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa
      541 ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct acaccatcgt aacgaatact
      601 acctccagaa acatcgtaga accgttcaag aaggttgatc agtgtcgtct tccctgcacc
      661 agtcggacct acaatagcga ccatctcacc aggtttgaca tccaagttaa aatcagtcat
      721 tagcaagtta tctggtgtgt agccgaattg aacattttca aaacgaactt tgtatggtgt
      781 atctttttct tctggaaggc cagatttttc attggacatc tcttcttcat ccaacacttc
      841 aaatacccgt tcggctgaag caactgttga ttggatagtg ttcatcaaac ttgcaagttg
      901 agtgattggt tgagagaact gtgttgtata ttgcaagaac gcttgaacat cccctaatgt
      961 catgttccca tttgcaactt ttactccgcc tagtacagcg acgaacacat agcctagatt
     1021 tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata aattgagctt tccaaccagc
     1081 ggcatacaga ttctgatttt ctttttcaaa tacttcctgg tctttttcag tatgattgaa
     1141 tgttttaaca atggtatgac caccatatgt ttcttctact tggttattca ataaacctaa
     1201 acttttttgc tgatctgcga aaaacacttg tgatttcggc gctacgatca tcacgatgat
     1261 caagcttaaa gggatcatca ataacgcgac taacgtcaat tgccagctga ttgaaagcat
     1321 catcacgatg accccaatca atgtgactgc actagttaca aattgagtca aattttgctg
     1381 cagcgtgctt gcgatgttat ccatatcatt gattgctcga gacatgatat ccccatttgt
     1441 atgtgtatca taataggaaa tcggtacacg attcattttg gcttccaaat catgacgtaa
     1501 ttcataaact gttcgttggg aaacacgtgt cataataaat tgctgtaaaa agttgaatac
     1561 agcagaaatc aaatacatca cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc
     1621 tatcggaaat tttgttactt gttggccttg ttgcatctgt tgagcgccag ccattacacc
     1681 tttgaatatt tctgtggttg ctttccccaa gacctttggt gtttggattt ggaagatcac
     1741 ggctgctatt gctagtacaa atacagcgat gatcgcaacc atacgtttag acatatagcc
     1801 aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt tcacctttta caccgagatt
     1861 tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt
     1921 tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg
     1981 tttgttgcta atagttcttc atgtgttcct ttgccaacca gttttccttc atctagcaca
     2041 aggatcatat ctgcatccat tactgtactt actcgttgag caactaaaac ggtgatactt
     2101 tctttcatat tcgttttcaa tgcttggcga agattggcat ccgttttgaa atccaacgct
     2161 gagaaggaat catcaaacac atagatatct gctgttttga ctaatgcccg tgcaattgcc
     2221 agacgctgtc tttgtccacc agagaagttt cctcctcctt gttccacatg gctatctaag
     2281 ccatcttcca tctctgaaac aaaatcttta gcttgtgcga tttctaatgc tttccaaatc
     2341 atttcatctg ttgcatccgg cttaccatac tgcatattgt ctcggatcgt tccagagaac
     2401 agaactgctt tttgaggaac aaagcccatt aattctcgca aattatattg agtcatgtca
     2461 ctaatgtccg ttccatttat ttggatcgaa ccagattcga tgtcatataa tcgagggatc
     2521 aaattgacta aggtcgtttt acctgaacca gtccccccta tgattgcgac gatctcgccc
     2581 gacttggctt ggaaatcaat atcttccaat gcaagttttt ctgctccatg atatcgataa
     2641 ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt tttaggattt
     2701 tcagggtcat ggataccgat tttttcatcc aacacttcat taatacggtc agctgaagct
     2761 tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca taaagctgat caagatctgc
     2821 atcgcataag tcataaatgc aatcagatta ccgacttcca aagtcatatc tgctatgtag
     2881 tgtccaccaa accaagtgat tgctacgttt gtaccgctca tgatcaatgt catcattggc
     2941 atcatcaatg cgacgatcgt attaactttg attgccgtat ttgtataatc tttattcgct
     3001 aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct
     3061 gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt tcttttgcat acttttgaat
     3121 aatggaacag caaagtacat gattccgccg atcaaaataa tcattactgg gatgacaact
     3181 aagaagattt ttgtcaactg atgatctttt tgataagcaa gtatgctcgc gccaatcaac
     3241 gtgattggtg cattgatcat catacgtaaa aacatttgtg taaccatttg gatttgagtc
     3301 acatcattgg tcgttcttgt gatcaaagag gccgttccat atttatcaaa cgcatctttt
     3361 gtcagactct ctgatttttt atagacatcc gtacgcaatt gcttacccag tttctgtgat
     3421 tcacgagtag caaaaaatgt atttgcgata gcagctaaga tactgatcaa agaaaagccg
     3481 atcatcacaa aacctgtatg ccaaatataa tcgacatctc cttgcgctac ccctttatca
     3541 ataatattgg aggtaagtgt cggcaaatat aaatctgcca gtacttggat caccatgaaa
     3601 actgcagctg caattgctga agtcaaggag atccgtttta cgatttttag catctagttc
     3661 ccttctttct atttatatac tccgtttttc agccaagaaa gtttcaaatt gaattcactg
     3721 attgcttgct ctactgagta atcttcagat gttgaaaata agcgatacga atgcgcaatt
     3781 gttgtaaata cagtattcat cactaattgg atcagtaatt ccaattcatg atcatttttt
     3841 actttcagct tcgtccgatc gatcaattca agcaactctt ttgcatcttg tttgtgttct
     3901 ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt tcttgaggca
     3961 cggtaatcca tgttcataaa caagttacga taaaaagaag cattctcgcc ttccaaaatc
     4021 tcataaatca ttttagaaaa ataggattcg aatcctttga atagatcacc atcggattct
     4081 ttcattagtt gtaacatatc tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa
     4141 taaagatcct ctttatcttc aaaatactga tagaaactgc ctctagggat atccgctaat
     4201 ttgacgatat tagcaatcga agcttctttt aagggtactc tagaaaactc gattctcgca
     4261 gcctcgatta gtcttttttg tttttcttta ggcaaatgga aaaatgtctg tgtcggcata
     4321 tggattctcc tttctttcaa gatgacacat tgtcatatga cgctatgtca ttataacacc
     4381 tcctacctgt tttgcaagac atttgcatag tttttttcat tatcatccga tacattaaaa
     4441 aagtgataag aggaatcctt ccatttgaat taatgagagt tgcccattaa aatagccaca
     4501 tactaaacaa aggatgagat aaatggccca atcaattagt gtccaagact tcaataaatt
     4561 gcctctcgat gctgacagca tcgttttaga tacaagaaat accttagatt acacaaaaaa
     4621 ccacttaagc cactcgcttt ctatccccgc tgctatcttg ccaagacaat taaaaaatct
     4681 agatccaaag aaactttatt atattctaag ctatacaggc aggcgatcag aagcattagc
     4741 cgatcaactt actgctaatg gttttcgtgc tgtttatatc attggtggga tggaagctct
     4801 tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa agaggctgtg acaaaaaatc
     4861 gtgtcacagc ctcttttata ccctgtcttt ttttagttat ttattcttca accttatcaa
     4921 tatgacttga tagccttttc tttttcgagt aaactgattt tgttagtcat agttataaca
     4981 atcatagcaa taaaaggaga aggtaagcat atggcatctg ttttagtagt caaaggtcat
     5041 cccctaactg cagaagaatc gcgcacagtt aaggcattga cgtctttttt aacaagttat
     5101 aaagaaaatc atccagatga tgaagtgact gttttggaac tttatcgcga tgatattccg
     5161 gaaatcgatg aagaattatt atcaggttgg gaagcattac gtgctggtgc agaattcact
     5221 tccttatcag aaagtcaaca acaaaaaatt gctcgtttca atgaattgac tgatcagttc
     5281 cttgctgctg ataaaatcgt tatcgcaaac gccttatgga atctaaatat cccaacaaaa
     5341 ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa cgttccgcta tacagaaaac
     5401 gggccagaag gattagtgac aggtaaaaaa gcactgcaca tccaatctaa tggcggtgta
     5461 tacaacggac aagactttgc ctctcaatat gtaaaaggta ttttaaactt tgtaggcatc
     5521 gatcaagtag atcagctatt catcgaagga atcgattatg accctgaccg tgcagatgag
     5581 ttgatgcaaa atgcattaga taaagctgct gctttaggta aatcatttta aaaattgaga
     5641 taaatgcata caaaagagac agaggaaaaa atcttcaaag cagcatttgt gccttggata
     5701 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
     5761 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
     5821 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
     5881 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
     5941 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
     6001 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
     6061 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
     6121 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
     6181 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
     6241 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
     6301 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
     6361 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
     6421 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
     6481 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
     6541 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
     6601 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
     6661 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     6721 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     6781 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     6841 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     6901 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     6961 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     7021 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     7081 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     7141 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     7201 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     7261 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     7321 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     7381 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     7441 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     7501 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     7561 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     7621 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     7681 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     7741 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     7801 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     7861 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     7921 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     7981 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     8041 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     8101 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     8161 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     8221 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     8281 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     8341 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     8401 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     8461 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     8521 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     8581 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     8641 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     8701 tttactcgat tttgagtaag tacaaggttg ttgttggtta taagtatgtc attatcataa
     8761 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     8821 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     8881 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     8941 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     9001 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     9061 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     9121 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     9181 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     9241 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     9301 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     9361 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     9421 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     9481 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     9541 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
     9601 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
     9661 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
     9721 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
     9781 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
     9841 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
     9901 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
     9961 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
    10021 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
    10081 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
    10141 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
    10201 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
    10261 tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
    10321 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
    10381 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
    10441 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
    10501 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
    10561 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
    10621 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
    10681 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
    10741 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
    10801 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
    10861 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
    10921 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
    10981 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
    11041 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
    11101 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
    11161 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
    11221 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
    11281 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
    11341 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
    11401 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
    11461 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
    11521 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
    11581 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
    11641 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
    11701 ccgctgtctg ccaaagcttc ctgagtcgat ccccctagat gaggcaacat aagtatacgg
    11761 tcattttcta agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt
    11821 tccaatgctt gcataacagc atatttatca acaatttcac ttcttcctac attgataagt
    11881 acagcatctt ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct
    11941 gtcaacggca gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct
    12001 tgccgaacat aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag
    12061 ctatagcaac ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt
    12121 ttgccttgca attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata
    12181 ttaggacctg ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag
    12241 agcaaacagc acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg
    12301 acgatcgtcc cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct
    12361 cttgaaattg ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata
    12421 actattcctt cggctggcaa atcatctatt attttgaaat attccccttg taacttttct
    12481 atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac
    12541 taacaagtat ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag
    12601 caaaaagagc agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac
    12661 tgccataagc cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg
    12721 ttccgttagc tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg
    12781 tcaatctctt cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata
    12841 accactgttt ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt
    12901 agcgagacaa cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg
    12961 aaattcggtt tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt
    13021 ggttcaaagg cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc
    13081 gatacgacta ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta
    13141 tctcccattg ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct
    13201 acttccatca tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa
    13261 tcttccatga atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct
    13321 gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca
    13381 gctagtccag gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta
    13441 tagcctcctt cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca
    13501 tgttcctccc atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac
    13561 tcttcaaata attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct
    13621 ccgataccaa aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct
    13681 tctgtgacac ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt
    13741 tcattatagg ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt
    13801 ttccagtagc caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat
    13861 tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa
    13921 ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc
    13981 ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact
    14041 tcttctcgat aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat
    14101 tcttgaaggc ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct
    14161 tgtgcatgtt tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca
    14221 acaaaccaaa attctttttc tccaatattc aacataatta gccactcctt ttatttgttt
    14281 tgtccgtaat acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga
    14341 ggcagctctt ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca
    14401 tctagtacga ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg
    14461 tgtccgtgta aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt
    14521 tctatgataa cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa
    14581 aatcgagcac aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga
    14641 acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct
    14701 ccgatttgag gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg
    14761 agtttacctt caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc
    14821 tcttcataac tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg
    14881 ctcacatttc cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg
    14941 tatacttctt ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg
    15001 aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc
    15061 tgctatttct tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc
    15121 tgccaagaag ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc
    15181 gattccccac gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc
    15241 agatgctaga atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa
    15301 acgatcaatt tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa
    15361 agcactggct aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg
    15421 tacaaataat ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata
    15481 agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa
    15541 taatttatct ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac
    15601 ccaagcattg atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga
    15661 aggtgtagtg accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat
    15721 catagcgaag gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt
    15781 agcgaccata ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa
    15841 atttcccgta gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc
    15901 tgctactaga actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat
    15961 taactgacca aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg
    16021 aaacatgcct gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac
    16081 ataacctgca agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat
    16141 agattgatac aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt
    16201 taatattcgt tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa
    16261 ctgttccttg ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc
    16321 gattgtcgta agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact
    16381 gacgcgcagt cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt
    16441 gttttcccac tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc
    16501 tttgatccta gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa
    16561 catactttgc gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg
    16621 atcaaaccgt ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt
    16681 caagcagtta tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata
    16741 aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat
    16801 acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg
    16861 cggcattcta tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca
    16921 tgattggggt ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt
    16981 aggtgcgatc cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa
    17041 aatgattctt ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc
    17101 tcacaatggt tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc
    17161 tgcatctgcc ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag
    17221 attgtcaggg atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga
    17281 ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc
    17341 tgtccccgcg ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct
    17401 tatgcaatct ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa
    17461 agaagctgaa caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg
    17521 aacttcttgg catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg
    17581 tgtagcagta ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat
    17641 cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg
    17701 aatcatttta gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg
    17761 aactttattg aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt
    17821 aggaactgta ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta
    17881 tgtcgcattt tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt
    17941 cccactagct gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc
    18001 tttcctagtt ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt
    18061 cggcattttt gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga
    18121 aactcgtggg aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa
    18181 acgtatttct atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat
    18241 aagaaggaac cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt
    18301 ttgatgaata ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg
    18361 tgaaagaaac gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa
    18421 ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt
    18481 ttcccgttca actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat
    18541 cgttgtgacg atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt
    18601 tgtcaatttt ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag
    18661 gtcatctgga atatgatagc cattttctgt caacttatct agcattaaac tagctatttg
    18721 atcgttatag caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt
    18781 ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg
    18841 cacattatac tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc
    18901 gtcgattttt gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc
    18961 cataaaaccg actttcacat catctaaaca aatataagag gcatttaact cttcataagc
    19021 agcattgatc atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt
    19081 tgggttatac tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat
    19141 cattttcttc aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa
    19201 tgaataccca gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata
    19261 atcagagaga tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc
    19321 tttcttatag ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc
    19381 atttaccaac acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg
    19441 cagcttgaat tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg
    19501 ttttatctct tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta
    19561 cgtacatata tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg
    19621 ctcataaggc atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa
    19681 agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct
    19741 ctggtcgagc atgatagatc aaaatatcaa agcacccgtc ttctgaaacc gtaaacgagt
    19801 tgtgaccagg accaaattgc ttattttttt tagaagaaac gaatactggc tccgataatt
    19861 tatgccatga ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg
    19921 catagttttc atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa
    19981 ggactgctgg accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag
    20041 aaaggaggat ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat
    20101 tagaattccc agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag
    20161 aagagaaagt tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt
    20221 ctatccaatc tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat
    20281 gatgagattc atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc
    20341 cattgatata atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag
    20401 cctgccatac aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta
    20461 attcaatcac ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt
    20521 tatataccca aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca
    20581 taacaaattt cctcacattt tctttattct aattacattc caagagtatt tttctatccc
    20641 tacatttaat tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc
    20701 tggttcttca cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc
    20761 ttgatctagc aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt
    20821 gatcaagaaa atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac
    20881 attttttatc ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc
    20941 tttagcatat ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc
    21001 tttagcatct gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt
    21061 tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc
    21121 catagtaaag taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc
    21181 cggttgggca tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa
    21241 atatcctttt acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata
    21301 agcttttgca aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc
    21361 ttcttcatca tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc
    21421 ttcttctaat gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc
    21481 aactaagatt gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc
    21541 taacctacca tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt
    21601 ccctaaacac cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc
    21661 actccagaag gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat
    21721 tccttttgtt cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt
    21781 aaaaaattcg tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt
    21841 ttttggtcgt tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt
    21901 tcctccagga tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg
    21961 tctaaacccg tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac
    22021 acttcccata tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa
    22081 atcctctctg atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc
    22141 tatagtcaag ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg
    22201 agggaaaaga aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct
    22261 caaaaaatca aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc
    22321 tttgttttca gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac
    22381 tacatatcca ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa
    22441 ataaataatg atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc
    22501 ttgaaagaaa aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata
    22561 aaaaacagca catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag
    22621 aatacctaat aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac
    22681 tattttcatc ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc
    22741 tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca
    22801 ggtccataaa aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc
    22861 atttttgaac tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc
    22921 gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa
    22981 atattaaact cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta
    23041 ataaaaaagg aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa
    23101 cctgcataag tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag
    23161 acttggaatg ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg
    23221 aaacgcatac gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact
    23281 gtccctccag caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa
    23341 accttaatat agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata
    23401 atgtcttttt cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc
    23461 aacaatataa ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga
    23521 ctattcacag taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat
    23581 ttgtttttct ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat
    23641 aaacaaaacc gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg
    23701 actgtaaata tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac
    23761 atatacttgg tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa
    23821 ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc
    23881 catttgagca gattcataca aagagggaga gattccctga atagcagtca agagaatcgt
    23941 catagtaaca ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt
    24001 tggatcgcga aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc
    24061 ccctacattc ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat
    24121 aactggaatg taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc
    24181 tactgctgct aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa
    24241 agaatgaatc aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa
    24301 acttacaaaa tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt
    24361 tcctaaaagc ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa
    24421 atacgcagca cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc
    24481 atctttcatt ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga
    24541 acaatcgctg tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat
    24601 atctgtatct tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg
    24661 attgatggca tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt
    24721 ttcaatcagt ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa
    24781 atatggctct gcatagtcca tttgtttcga taattcagat acaataggat cattttttac
    24841 ttcttcatct tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt
    24901 tttaccaatt tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt
    24961 attatctgta cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc
    25021 tgaatcactc ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc
    25081 gttatttaac catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc
    25141 catttcggcg ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat
    25201 cgcttttaac gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt
    25261 cactaattta ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt
    25321 gaagacaaaa cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa
    25381 ttcttcccaa gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt
    25441 attccaatac atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac
    25501 ttcacctaat tcaatagccg tttctttaaa atcagattta tctactccat catattccca
    25561 gaaatcgtct aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt
    25621 taatgcaata aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa
    25681 attggcccaa gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa
    25741 ttcattaacg atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt
    25801 caaagtcact tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa
    25861 aatacctgtt gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg
    25921 ttcctccttc atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac
    25981 aattgatttt cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac
    26041 ttttaggaag attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc
    26101 cctctaatga taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa
    26161 ctgcaaatat gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt
    26221 cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt
    26281 gtccatacac agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt
    26341 ttgtttctgt cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta
    26401 ctctatctgc atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa
    26461 aattatagtt atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt
    26521 gttccgctga atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac
    26581 tatgtttctt cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca
    26641 tgtctaagga acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat
    26701 gcagagacaa ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac
    26761 caaatgtagg catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta
    26821 cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat
    26881 gtccaatctg ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag
    26941 ggctctcttg accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac
    27001 aatattcgac taaattacga gctgcatcta ctccacgagt acccagattg acagccatat
    27061 tgatttcggc ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg
    27121 tttcaattgt ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac
    27181 tgtcttccca acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt
    27241 tcaattcttt taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat
    27301 gatctggctg ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt
    27361 acaaccgcgg atcaactttg cctacacgct tgacactact aatcaacatt gtggcttcca
    27421 taactgacct cctttaattt atacatgaat aatacacctc gttgaaagcg ttgtcaataa
    27481 tataaatgtt aaacttactt attttttatc gattttaatt aaccaaaata ttaataatga
    27541 ttaaaacaaa taatttaata taacttttat taacaaatat gttaatatat aagaaaaacc
    27601 ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa
    27661 gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat
    27721 gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa
    27781 aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc
    27841 cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg
    27901 aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt
    27961 ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct
    28021 cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct
    28081 aattttttaa atacagagga tacattggag atgttggaag tttctgtgga aatttcttct
    28141 gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg
    28201 gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct
    28261 gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa
    28321 cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa
    28381 gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc
    28441 tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac
    28501 tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg
    28561 acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg
    28621 tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac
    28681 ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc
    28741 tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc
    28801 cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg
    28861 caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc
    28921 atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat
    28981 acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct
    29041 taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg
    29101 ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag
    29161 acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt
    29221 tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa
    29281 agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat
    29341 ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag
    29401 gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt
    29461 tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc
    29521 ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct
    29581 ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt
    29641 aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg
    29701 aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct
    29761 tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt
    29821 actttttctg cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg
    29881 atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt
    29941 gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct
    30001 tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt
    30061 tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag
    30121 ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac
    30181 gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat
    30241 gcttcataat catttccata agattggact aacgaatgag catgggtccc gctagctggt
    30301 atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc
    30361 gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat
    30421 agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg
    30481 aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc
    30541 acttggatta atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt
    30601 gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca
    30661 cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt
    30721 tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca
    30781 aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga
    30841 tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac
    30901 tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa
    30961 atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact
    31021 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat
    31081 tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa
    31141 aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat
    31201 aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt
    31261 ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt
    31321 ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca
    31381 cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg
    31441 gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt
    31501 accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc
    31561 actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc
    31621 actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag
    31681 tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca
    31741 tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata
    31801 gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta
    31861 ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag
    31921 atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac
    31981 ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa
    32041 aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag
    32101 gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga
    32161 gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag
    32221 gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa
    32281 caatggcatt caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc
    32341 agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc
    32401 tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca
    32461 aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac
    32521 aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga
    32581 atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc
    32641 tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc
    32701 ataagaagtg gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag
    32761 gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg
    32821 gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca
    32881 acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt
    32941 tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata
    33001 tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa
    33061 caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag
    33121 aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata
    33181 gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag
    33241 ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac
    33301 aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt
    33361 ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt
    33421 caaataagta agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg
    33481 ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc
    33541 atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc
    33601 ttcaagatac cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta
    33661 atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt
    33721 ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt
    33781 tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc
    33841 atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag
    33901 ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct
    33961 tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc
    34021 gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc
    34081 attggtattt cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg
    34141 gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct
    34201 acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt
    34261 ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa
    34321 agtctcttat tttaaattga atacattata atgatactgt aaatatatct ttattattag
    34381 tgaatggagt caagtccaca atcttgtgta aaagttcaac ctcatcaaaa gcacaatgtt
    34441 ttgcgttgtg aacctaactg ccatgccttt ctttctgagg agagaggtca ggagcagtag
    34501 cgcagcgact cttgatgtct ctcctcagaa agaagagact cgcacaggtt catggtgcta
    34561 aatgttattc gaattaaatt ctaaatattt tcgtggagaa gccagccact cagtgtcttt
    34621 ttccatcaaa agtgttccaa ttatccgagt gatggaattg atatttggaa aaatccgaat
    34681 cactcgttct ctccggcgaa tctcttcgtt caaccgttcc aacacattgg ttgtacgaat
    34741 ccttcggcgg atattttcag gaaaagacaa aatttgaata gcatcttcaa agccgttttc
    34801 taaaatgtcg caagcagcac ttaattttga atcacaacca tatttttcca agaagtgctc
    34861 ttttctttca cgtgtcaatt caagttcaga ggctttaaag atactcttca actcgtcttt
    34921 tacatcagaa gaaactttct ttggcagctt atcaaaaata tttctcaaaa agtgggcttg
    34981 gcatctttgc cagctcgcac ctaaaaagtt cttgcgaaca gccttaacta accctttgtg
    35041 tgcatcagaa atgaccagtt ttacaccgaa caaacctcgt tgaacgaacg attgaaaaac
    35101 ggtatcccaa ttatcttccg attcgccgtc ttgaacatca aaaccaagaa tcgtacgtcg
    35161 tccatcagaa tcaatgccga taccaatata tacacctttt gagacaatac gatgattttc
    35221 ccttactttc atatacaggg catcacacat gaaaaatgga tattccgagc cttctaggct
    35281 ccggtttctc cactcaaaaa caaatggatc caactgtttc atcagatttg aaacgaatga
    35341 tttagaaaca gtcgcaccat ccgttagcag ttcaactgtt ttagttactt tgcgagtgga
    35401 gacaccagaa atgaccattt caatcatggc agcgatcaaa gctttttcgc ttcgctgata
    35461 tctttcaaag atagtaggac taaacttacc gtctctggtt cgtggaacat gaagttctaa
    35521 cgtaccaatt cgagttgtaa acgaacgatc gtagtaacca tttctttgtg attgccgttc
    35581 ttctgaacgt tcataggcat ttgcctgaat atattgattt cgttgttctt ccatcagctg
    35641 attaaacatg atagtcataa gtgtttttgc ggtatcattc gcaccgcttt cgtttataat
    35701 tgcttgaatt tcttcgcttt caaaagtaaa atgtacttga gtcattgata tcagtcctat
    35761 tcgagtatgt tttctgcaaa aaacattgta ctcctaactg agatcaatgg cttttttgat
    35821 gcttcacttt tacacaatta tatggacgta atctacgcca aaaactgtta aaaaactttt
    35881 actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag
    35941 gaagtagact aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa
    36001 aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgcttct
    36061 ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct
    36121 gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt
    36181 attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctatg tggagatcca
    36241 tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt
    36301 accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaagtcg cttacagtat
    36361 gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt
    36421 ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat
    36481 caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt
    36541 cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga
    36601 agtaaagatc cagaccgaga agactttttc gggggagatt tgcaaggtgt gatggatcat
    36661 ttggattact tgacggacct tggcatcaat ggcatctatt tttgtcccat atttaaagcc
    36721 acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat
    36781 aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc
    36841 gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac
    36901 ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat
    36961 cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact
    37021 ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc
    37081 gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa
    37141 gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc
    37201 tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat
    37261 gcagtgatga attatgcatt tacagattcg attaaagatt attttgcaaa aaagaaaatc
    37321 acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg
    37381 aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt gaccctttgc
    37441 caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct
    37501 ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc
    37561 aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa
    37621 cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta
    37681 atagtaaatg atcgtgaaga taagctctat ttcacacgga aactagaggg acagatcatc
    37741 tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt
    37801 cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt
    37861 ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt
    37921 tgtgcccatt ataataaggg tgtaacagca cgttgaatac aaaggaggaa atgaagatga
    37981 aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat
    38041 tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt
    38101 taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg
    38161 aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat
    38221 cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc
    38281 ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag
    38341 cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg
    38401 cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa
    38461 cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag
    38521 gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg
    38581 caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga
    38641 ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc
    38701 caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca
    38761 ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata
    38821 tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg
    38881 gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat
    38941 ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa
    39001 tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag
    39061 ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag
    39121 tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag
    39181 aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa
    39241 aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga
    39301 gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag
    39361 caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa
    39421 aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa
    39481 aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt
    39541 tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt
    39601 tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg
    39661 tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa
    39721 tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac
    39781 acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt
    39841 cactgcattt actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt
    39901 aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc
    39961 agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg
    40021 tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat
    40081 cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat
    40141 attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc
    40201 ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat
    40261 tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt
    40321 acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca
    40381 gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac
    40441 acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca atggcggtcc
    40501 cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact
    40561 tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt
    40621 catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga
    40681 ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt
    40741 acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca
    40801 agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg
    40861 actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat
    40921 acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc
    40981 tatacttata gtcgctatcg t
//
LOCUS       DANKHJ010000018        39068 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000018 DANKHJ010000000
VERSION     DANKHJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..494)
                     /locus_tag="PW113_001084"
     CDS             complement(<1..494)
                     /locus_tag="PW113_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDL2525412.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETS"
     gene            complement(491..1231)
                     /locus_tag="PW113_001085"
     CDS             complement(491..1231)
                     /locus_tag="PW113_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2525413.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(1244..2602)
                     /gene="rsmB"
                     /locus_tag="PW113_001086"
     CDS             complement(1244..2602)
                     /gene="rsmB"
                     /locus_tag="PW113_001086"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2525414.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(2602..3540)
                     /locus_tag="PW113_001087"
     CDS             complement(2602..3540)
                     /locus_tag="PW113_001087"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2525415.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(3533..4024)
                     /gene="def"
                     /locus_tag="PW113_001088"
     CDS             complement(3533..4024)
                     /gene="def"
                     /locus_tag="PW113_001088"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2525416.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(4039..6447)
                     /gene="priA"
                     /locus_tag="PW113_001089"
     CDS             complement(4039..6447)
                     /gene="priA"
                     /locus_tag="PW113_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2525417.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(6700..7005)
                     /locus_tag="PW113_001090"
     CDS             complement(6700..7005)
                     /locus_tag="PW113_001090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2525418.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(7010..7624)
                     /gene="gmk"
                     /locus_tag="PW113_001091"
     CDS             complement(7010..7624)
                     /gene="gmk"
                     /locus_tag="PW113_001091"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2525419.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(8111..8395)
                     /locus_tag="PW113_001092"
     CDS             complement(8111..8395)
                     /locus_tag="PW113_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525420.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(8413..9303)
                     /locus_tag="PW113_001093"
     CDS             complement(8413..9303)
                     /locus_tag="PW113_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2525421.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(9323..9541)
                     /locus_tag="PW113_001094"
     CDS             complement(9323..9541)
                     /locus_tag="PW113_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2525422.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(9511..11211)
                     /locus_tag="PW113_001095"
     CDS             complement(9511..11211)
                     /locus_tag="PW113_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2525423.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            11478..12251
                     /locus_tag="PW113_001096"
     CDS             11478..12251
                     /locus_tag="PW113_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525424.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(12332..12901)
                     /gene="thiT"
                     /locus_tag="PW113_001097"
     CDS             complement(12332..12901)
                     /gene="thiT"
                     /locus_tag="PW113_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2525425.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(12967..13069)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13075..14046)
                     /locus_tag="PW113_001098"
     CDS             complement(13075..14046)
                     /locus_tag="PW113_001098"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2525426.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(14140..15513)
                     /gene="glmU"
                     /locus_tag="PW113_001099"
     CDS             complement(14140..15513)
                     /gene="glmU"
                     /locus_tag="PW113_001099"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2525427.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(16016..16834)
                     /gene="purR"
                     /locus_tag="PW113_001100"
     CDS             complement(16016..16834)
                     /gene="purR"
                     /locus_tag="PW113_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2525428.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(17001..17825)
                     /locus_tag="PW113_001101"
     CDS             complement(17001..17825)
                     /locus_tag="PW113_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2525429.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(17798..18478)
                     /locus_tag="PW113_001102"
     CDS             complement(17798..18478)
                     /locus_tag="PW113_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2525430.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(18501..19439)
                     /locus_tag="PW113_001103"
     CDS             complement(18501..19439)
                     /locus_tag="PW113_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2525431.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(19684..20547)
                     /locus_tag="PW113_001104"
     CDS             complement(19684..20547)
                     /locus_tag="PW113_001104"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2525432.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(20689..23262)
                     /gene="mprF"
                     /locus_tag="PW113_001105"
     CDS             complement(20689..23262)
                     /gene="mprF"
                     /locus_tag="PW113_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2525433.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            23403..24209
                     /gene="proC"
                     /locus_tag="PW113_001106"
     CDS             23403..24209
                     /gene="proC"
                     /locus_tag="PW113_001106"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2525434.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(24288..25844)
                     /gene="rny"
                     /locus_tag="PW113_001107"
     CDS             complement(24288..25844)
                     /gene="rny"
                     /locus_tag="PW113_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2525435.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(26255..27304)
                     /gene="recA"
                     /locus_tag="PW113_001108"
     CDS             complement(26255..27304)
                     /gene="recA"
                     /locus_tag="PW113_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2525436.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(27413..28654)
                     /locus_tag="PW113_001109"
     CDS             complement(27413..28654)
                     /locus_tag="PW113_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2525437.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(28894..29472)
                     /gene="pgsA"
                     /locus_tag="PW113_001110"
     CDS             complement(28894..29472)
                     /gene="pgsA"
                     /locus_tag="PW113_001110"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2525438.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(29681..30604)
                     /locus_tag="PW113_001111"
     CDS             complement(29681..30604)
                     /locus_tag="PW113_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525439.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(31343..32629)
                     /locus_tag="PW113_001112"
     CDS             complement(31343..32629)
                     /locus_tag="PW113_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2525440.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(32622..33890)
                     /locus_tag="PW113_001113"
     CDS             complement(32622..33890)
                     /locus_tag="PW113_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2525441.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            34142..34603
                     /gene="mscL"
                     /locus_tag="PW113_001114"
     CDS             34142..34603
                     /gene="mscL"
                     /locus_tag="PW113_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2525442.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(34662..35108)
                     /locus_tag="PW113_001115"
     CDS             complement(34662..35108)
                     /locus_tag="PW113_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525443.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(35201..35446)
                     /locus_tag="PW113_001116"
     CDS             complement(35201..35446)
                     /locus_tag="PW113_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2525444.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(35561..36139)
                     /locus_tag="PW113_001117"
     CDS             complement(35561..36139)
                     /locus_tag="PW113_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2525445.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(36216..36746)
                     /locus_tag="PW113_001118"
     CDS             complement(36216..36746)
                     /locus_tag="PW113_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2525446.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(36750..37610)
                     /gene="rlmB"
                     /locus_tag="PW113_001119"
     CDS             complement(36750..37610)
                     /gene="rlmB"
                     /locus_tag="PW113_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2525447.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(37603..37998)
                     /locus_tag="PW113_001120"
     CDS             complement(37603..37998)
                     /locus_tag="PW113_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2525448.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(37995..>39068)
                     /locus_tag="PW113_001121"
     CDS             complement(37995..>39068)
                     /locus_tag="PW113_001121"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2525449.1"
                     /translation="VMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQ
                     SIDELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMA
                     TKHLGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSK
                     SLGNFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLK
                     FRQETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQP
                     KVSAAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIR
                     DLLKEKGILLEDTPQGTRWRREA"
BASE COUNT        10805 a         8508 c         6855 g        12900 t
ORIGIN      
        1 atcgatgttt cagataaagc gatagcaatc ccgaaatccg tgatttttac aacaccttca
       61 ttatcgatca gtacgttttg gggtttcaaa tctcggtgga tgatttgatg gttatgagct
      121 aaagaaatag cagaaaggat ctgctgcata atatttactg ctgtttcata aggaacagga
      181 taatgtgttt ggatatatcg tttcaagtcc attcctttga cgtattccat caccagatac
      241 tgcatgttgt cttcttcacc aacatcgtaa acactaacaa tgtttggatg aacgagttct
      301 gttgctgcta aagcttctct ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca
      361 aatcgtaaga ctttaactgc tacatcacga tccaagatta aatcgtgcgc caaaaataca
      421 tttgccatac caccgctacc gatatttcca gtgatttgat aacggccatt taattttttg
      481 cccaactcga tcatacactg tcctccttat attcgataag aagaacggtg atattatccg
      541 ctccgcctgc ttcattggca cgatcaatca gctcagttac tttatgagac aatgggcctt
      601 cttgattgat gatcgttcct atttcttctt cagacagcat gttcgtcaag ccatctgaac
      661 ataacagcag tcgatctttt agttgccaaa gatgagtaga aacatctact tcgactgttc
      721 caggcatgcc gacagaacgt gttaaaatat ttttccttgg atggttcact gccatttctt
      781 cgctgatttc tcctgatttg actagttcat tgactaaaga atgatcctcc gtcaattgca
      841 tgattttatc ttcacgaatc aaataagctc tgctatcccc aacattggca atcgtgaacc
      901 agtcatctga cagagcggcc gctacgattg ttgttcccat accgttatat tccggctgtt
      961 cttgtccacg ctgataaatc agcatatttt cttcttgaat cgattgaatg aaccattggg
     1021 cgattttttc atcgtcaaca gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg
     1081 ccatctgact ggcaatgtct ccggcacgat gaccacccat cccgtctgct aatacagcaa
     1141 gcttgagacc tgaacggctg gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc
     1201 ttcctacatc tgattgatat tcaatctgca ttttttcacc tcattacttt ttgcgcaaac
     1261 aacaaatgaa aaatccatct gtgtaaaact gatgcggata caaagtcaac attttctctt
     1321 cgattgatga ttgtagcata ggattggtca aaacatcaat cctctcaaaa tcagggtgtc
     1381 tttccaagaa agcagaaaca acgtcttggt tttcttcttt taagatcgta caagtgctat
     1441 aagtcagaat accggaagac tttaaagttg gtgcgcagct ttctaaaatt tccaattgga
     1501 tttttggtaa attcgctaat tcatccggct gttttttata acgaatatct ggttttcgcc
     1561 gaagcagccc aagacctgaa caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt
     1621 cgaactgttt actgacttct ctagcatcca tcttttgagc gtagacagca gaagagacac
     1681 ctagacgtgc tgcattatct tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga
     1741 ccacttctcc accgcatgac ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg
     1801 gtgctgcaca ggcatctaag acatgatgtt caggctcgat ttgcatagac ggcgctacaa
     1861 gcatcgaact ttcatcttgg atcgtcatga cgccatcgtg gaacaatgag ctagaagcta
     1921 aatgcccttt tttcgctact ataccgtatg gactgacttg gcttttttca gctgtgatct
     1981 cttcttcttg caaaacagtg atggcctgat ctctggaaag tcggcgtgta tctactcggg
     2041 cactagcatg gctaggctga tatagcgata aaccgagctt ttcagtttct tcgtatccga
     2101 tttgatccac cagtcgtttc gtcatccata gaggcaggct gattttgaca gaaagtcttt
     2161 cgataggatc agcaatgcct tgcagatctg gtgctccttg acgctgataa ttcctcaaga
     2221 caccatttac aaatttccct gtcccaggat ttccttttac ttttgcgatt tcgaccgcct
     2281 cgtttaaaat agcatgtgca gggactttat ctaaaaattc taattggtat acagaaagat
     2341 aaagaagcat cttgacccag tcatccactt tttttgcttt agcgatcaat ggtgcaacgt
     2401 aaaattccaa tagtcgctga tggctaatcg taccatagat caattctgta aataaaccca
     2461 catctttttg atttagctcg ctttgtttga tcatctcatt caacaaaaga ttcgagtagg
     2521 cgccaccctt gttcactcgc tctaatgtca gtaatgccat atatcgtact gattttttca
     2581 tttttttagg gatttttttc attatttcac ctgctgacca atctgaactt gctgaccgct
     2641 tccgttaagg aaatcatgta cggcctgttt tcctttacca gctggctgta attcattgat
     2701 ggctaagacg gtctgtttac cgcaagcaat ccatagattt tttttgtctt tccggatgat
     2761 cgtaccaggt gctttgtctg tagtttctgc caatacttca accgataata atttccagcg
     2821 tatgccctca taggtcgtga atgcaattgg ccaaggacgc attccgcgta cttggttgtc
     2881 aatttcttct gcagttttat gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga
     2941 aaaagttgct tctgactcgt cttgaggtac tggtttttgt ccatccaaaa tattgggcaa
     3001 tgtttctagc aatagctttc ttcctaaaag gctaagcttt tcaaacatcg tccctacatc
     3061 atcttgttta gtgattggta tgctttcttg cgcatagatt ccgcctgcat ccattttttt
     3121 gatcatttcc atgatcgtta caccagtttc tttctcccca ttcatgatgg cataatgaac
     3181 aggcgcacct ccccgatatt taggaagtaa agaagcatgg acattgattg cccctaattt
     3241 tggtacttgc aataactttt caggcaagaa ttggccaaat gccgcagtga cgatcacatc
     3301 aggcaccaat tcaatgattt cttccatttc tggcgaaccg gagattttct ctggctgcag
     3361 cacacggata ccgtgcttca gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt
     3421 ccgcccaact ggacggtcgg gctgtgttac cactgcctgg atctcgtaac ctgattctat
     3481 cagcccttca agaatcggca cagaaaaagc aggtgtgccc ataaatacta gcttagtcat
     3541 caagatgttc ctccatatat tcttcaagtt cttcttccgg gatctgctgt atcattttct
     3601 caatgaacaa gataccgtct aagtgatcga tttcatgctg gaaggcacgg gctaaatagc
     3661 caaaagccgt cacttctatc tcttctccgt ctcgatcata ataacgcacg gtcacttcat
     3721 ctgctcgctt gaccgttcca tagacatgcg gaatactgag acaaccttct acgtcaaggc
     3781 tttcaccttt tgcttcgatg atttctgggt taatcagttc aaatttctcg ccttcatcta
     3841 cttcgatcac agcaattctc ttgttttgtc ctacttgagg agcagcgatc cctattccgt
     3901 cattcgcaat cattgtctca tataaattgt ctaataaagc gattgtctcg tccgtgatta
     3961 catcgattgg ctgagcagtt ctttttaatt tatcattagg gtggatcaat ataggatagc
     4021 gcataatttg gccctttctt aaataaagtt taatggttcg ttgtcaattg ccacaaaaag
     4081 accttgtcgc tgttctttct ggcttccatt caaaatttca tccaaaacat gaggaagatt
     4141 cggttcatgt ttatatttga tgattagttg ataataatat cggtttttca ctctcaaaat
     4201 catgctgggc gttggcccta atagcaagct ttgagggctt aacacttctt ttagctgatt
     4261 ggcaatctga aagattttct ttgccgctac ttgttcttca ggatggctgg cggttatttt
     4321 cacagtaaag taataaggag gatagcctcc acgatgccgt acatgcattt cctgctgata
     4381 aaactgctca taattttgtt ttttagccaa ttctatggcg taatgttgag gattgaatgt
     4441 ttggatcacg acttcgcctg ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag
     4501 caattggaat gtatgctcac tagaacgaaa atccggtaaa ttcagggagg tatcagcatt
     4561 caacacccca actaatgtca cttctggaaa gtccaaacct ttagcaatca tttgtgtccc
     4621 taataatata tctgcttctt ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt
     4681 tcttcgagtg gtatctacat ccatacgcaa aatcctagct tgcggataaa gttctcggag
     4741 ttcctcttct actttttgag tacctgttcc atagtagcgg attttattgc cgccacaatt
     4801 aggacagcga ttgggaatag gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt
     4861 atccatatgc agtgtaagag agatatcgca attaggacat ggaagaacaa atccacagtc
     4921 cctacacatc acaaaagacg aatatcctcg acgattgagc agtaggacag tttgttcttt
     4981 tttgtctaat cgattttgta ttttttcctg caaattagca gaaaaagtag aggtccgatg
     5041 attctgaaac tcttcacgca tatctacgat ctcaatagct ggaagctgtg cagctgcttt
     5101 ggctcggtgg tttaactgca ataactgata aacgcctttt tgagctcgtg ctcttgattc
     5161 taaagagggc gtcgcgcttc ctagaactat tgggcaatga tgatatttac tgcgccaaat
     5221 tgctagatct ctcgcatgat aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc
     5281 ttcatcgata atgatcagcc cgatattttt cagcggggca aaaatagcag atcgtgcgcc
     5341 tactactact tgcgcttctt cccgctcaat cttgcgccat tcatcatatt tttcaccttg
     5401 ggaaagtccg ctatgtaaga ccgcaactga ttctccgaag cgtcctttga atcgctcgac
     5461 catttgcgga gtcaatgcga tttcaggcac taacataata gccgttttat tttctgacaa
     5521 aacatcagcg attgcctgaa gatatacttc tgttttcccg ctgccagtga ttccctccag
     5581 caaaaagacc tgatcttttt gctgctgacc tgctgtgatg atttgttcga cagcattttt
     5641 ttgttctgca ttcagttcca atgccgtcgt ttggtcaaat actcggtctt tataagggtc
     5701 gcgataacgt tctgattcta taaactccag ccagcccttt tcttttcctt gattcaagac
     5761 agcagtactg attcctttat ttttgaaata agcaattggt tcttcttggc tcagttcaga
     5821 taaacacaaa agaagttctt tttgtttctt ggcattttgc cgtaattttg cccattcttc
     5881 ttcgatttgc ttttcagtta acaaagaacg gaccaaacga atcgttttta ctttatttcg
     5941 tgtatggact tcatatttga tatctactaa ttgctcttgg cgccagcgaa tcaattgaga
     6001 aaggctgcct tcttcttctg cttccttcca ggaaatcact tctcgctgat caaacaatac
     6061 ctgaacttct ggtgtgtctg ccaaaggata gatcaattta tcatattctg ctttcatcac
     6121 actcggaagc atcgtttgta gacaagtgat tttgaatgcg taagtaattt ttttcatata
     6181 atcagctaat gaaagtaatt cttcattgac caccggagca aggtctagca aacgaataac
     6241 tgccttcaaa gatggattag ctgattcttc tgatttttgg atagccataa caaatccttg
     6301 gacatgacga ttgccattac caaaagggac ttctactcgc atcccgactt gcaaagctgt
     6361 ttccatttca gagggaacca aataagtgaa tggctgatcg gtctgcatag taggtacgtc
     6421 cacaatgact tcagctgtta atgtcatggt tcgtttcctc ctttttttgt cttttcttat
     6481 caagcgacaa aaagttcctt gcctatttta ctagagatcg atggatttgt cttatcttct
     6541 gccaaatcct aacaaaaaat taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt
     6601 cagacacagc cttttctacc agacaataag tggcgaatgt ttgaaaagga aacagctggg
     6661 tttgcttttc tttttagatg ttcgctctat ttttctttat tacatttttt gctcttcacg
     6721 gatacgtgat tccaattcgt gttgttcacg ctctttttgt gcttgacgtt cctcttcttc
     6781 catcttcaaa cgttcacgtt ttaattcagg atgtggatca ttgactacat ttcctgcatc
     6841 gatttcttct aaagcttgac cgacgctttt tactgaatca aaagaatcta gagtaggctg
     6901 tgcacttgca tccaattcat gcgcgcgttt acttgctaaa atcactagag aatattttga
     6961 atttacacga tctaatagtg agtcaattga aggttttaac atcataagac tacatctcct
     7021 ttaacatttt aatatattta ccgattacgc gatctactcg aaaatgttca ctggcaataa
     7081 tgtttttgat tcgttcaact gctagcggca cttcatcatt tacaacagca tagtcgtaaa
     7141 gagccatcat ttcgatttct tcttttgcaa ctttcattcg tttttcgatt acttcatctg
     7201 catctgttcc tcggcctacg atccgtgatt ttaattccgc taaatctggc ggagtcaaaa
     7261 agatgaatac accatcgggc actttttctt tgacttgttt tgctccttgg acttcaatct
     7321 ccaagaaaac atcttttcct tcatccaatg tcttgttgac atatgacaaa ggagttccat
     7381 aataattccc gacatattct gcgtattcca gcatctctcc agcttcgatc attgattcaa
     7441 actcttcttt gcttcggaag aaataatcaa cgccttcgac ctccccttca cgcattttac
     7501 gggtagtcat tgagatcgaa tactgaaaat cattatcctc actctcaaaa attgcttttc
     7561 gtactgtgcc ttttcctaca ccggatggac cagacaacac gattaataat ccacgctctg
     7621 acatgacgac gtcctttcaa ctagtctttt tgtacttatc gtttattttg cacttttttt
     7681 ctgcaacttt caaggttatc tttaattata gctagaaatt ggtaaaaaag ctaccctgta
     7741 ccaagaggtc gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa acataaacag
     7801 cttctcatgt ttttcgtttg gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt
     7861 gtataagagg tcgtgaagaa gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat
     7921 agtttctcac atttttgcat aaatttggct taataccgag aaaatggttt ttgaacatca
     7981 cttattcaaa ataaaaaaag attgtgacac gaattttgtc acaaccttta aatgtattcc
     8041 tagagcaaag aagctaagcg aagaaatcca attctgttta tttattgtat ttctttgttc
     8101 agcatccttg ctaaaagccc atattggaca aagtattcac tacacccatg atctcatctt
     8161 ttcgagaacg tgctttttct gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa
     8221 atttccgatt catcgtcacg taagcaccgt cgttgatcaa aataaacagt tcgttctcat
     8281 tatagtctgc acaggcatct ttccatacgt caaagccgtt tttttcagaa gggatatccc
     8341 gtttaaccac agtttttcca cttaattcgt ggaacgttac gtcataatat ttcattatct
     8401 ctacctcttt cgtcattcaa tattttgtac ttgttctctg attttttcta agatcgtctt
     8461 agactggacg acttgatttt taatttcgat tgcatttgat ttggaaccga tcgtgttgat
     8521 ttcccgattc atttcttgga tcaggaaatc taattcccga cctacaggac attgtgcttc
     8581 gataagttca tctagttttt gtacatggat cgccagacga tccagttcct cgtgaatatc
     8641 tccgcgttcc aacaagatgg ctagctccgt caacaaccgt tgctcatcga ctttcgctcc
     8701 aagaaattcc tccagctttt tttgatacct tatttgaaat tccttttcat agatttctac
     8761 aaaactatcg agttttttta gtacttcttt gaattcttca gtattctttt tcaacactaa
     8821 agctaaagct tgaccttctt tttctcgact cgcctcaata gaatcaagtg cgcttttcaa
     8881 tgtatctaga gctaactgtg ccaattcttc catgctctct tccggttttt cttctaccgt
     8941 tacgaagttt tcgtccagca cgaagcgttc cgccatccga ctaattgaca cttgattttg
     9001 actaccgtat cgttgttgaa ttccgtctgt caattgagtg ataagtgaat caatcaattc
     9061 ccagttgatg acgatctgtt tttcgttttt gccaatataa gctagattca caaatacttc
     9121 gattcgaccg cggcttaact gctgtttaat aagctgacga aaatcgtttt caacaaagtt
     9181 caggatttta ggacttcgta tttgaacatc tagaaaacgc tggttgacac tttttatttc
     9241 aatatctaac tgatacgctt ttgtttcgcg gatggctttg ccaaaaccag tcatactttt
     9301 cattcgacag ggtccttcct cattacatat tttctattgc tttttgcagt tgttcaaact
     9361 cttcgttcgt caatgtcagg cctttcccca ttttttcatg gtctggcgcc caatctcgaa
     9421 gatcgaattt tggcgggcga ccattccagc tgactaggtt cagttcttta cgccacccct
     9481 tgttgttttc tgacaatata gcgatttctt ctacgatttc atatgaaaat tcttgtgcca
     9541 tggttgttcc tccttgagat aatataaata taggatcagt aatgtgtcaa ctggttgctt
     9601 aagcaattca tgctgcaaac gatccttgcg ttttccttgt agcagataat caaaataaac
     9661 agttttcgtg cgctcatttt gtattttttg ctggtatttt cgataccttc gccattcttc
     9721 agtacttaat tgaccaattt gtgccaataa atcatccggc atacttttta tatcttgttg
     9781 gcagatcatt tgaatcatca tgtctacttg atagatttca ttgggcagta aaaaatgttt
     9841 ttttgctaaa agttctagca tcgccaatcc gcatgtctct ggtacgaccc tgttcttata
     9901 taaattacgc agtttttgcc aaaattttat gcttttgaaa aaagataatt ctaactgaat
     9961 ttcttttgtt aagtaagaca aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg
    10021 aaatgtatac cagttcccat agtaatagcc taatcgtttt ttcatttcca gatagcctaa
    10081 ttgtaaattt aattggttcc tttggctatc aaaaagcaga aagctgccta gtgtccacaa
    10141 tgtttgacat ttccaatgga taaaaatgtc tggcatacga atttttttcg ctgggcctgg
    10201 cccttgcaca tctacgacaa atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg
    10261 tattttgttg cgatacccac cgtctgcata tttatttttt ccgatctttc gataagccat
    10321 agcgggataa aatgaagcag aggcgagtat ccaatctggt atttcttctg tttttgtctg
    10381 ttggatatca gtcactactt ccgctagttc tggcagttta gtagacaccg tatataaacg
    10441 tattttggga ttatgtttga gtaaggacaa atccaaattg tctttgacca tcttttccaa
    10501 tggagcagaa gaaaccccgc cttcaattag tgctgttttc gtcatttggc gtctttctcg
    10561 atagaagcgt tcacgaaggt cttcaactgc agccatttct ggtaacgcaa gcacttgtct
    10621 tgtcgacagc tttttccata aattcacagc ttttttcata tctccttgta aaatcaatgc
    10681 gccgttcaac gcacctaccg aggtacctgt gataatctca aagttgatat catgttcctt
    10741 cagcgcctgc cacacaccaa tctgataagc accgtgcgct cctcctctac ctaaaacaag
    10801 tgctgtatga tagaccagct ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg
    10861 aaaaatacgc aaccattctt ctgatagagg agattgaaat aaaaaacaac agttttcttt
    10921 aataaaaaaa cgagcgcaag cttccatata ccgaaaaaat tttttccatt caaaagtttc
    10981 accttcaatc aacagattat taaaaataac atctcctcta cttttagcaa tagtaaaata
    11041 gacggtatat tttttcgata ctgctttaaa agcacattgc ttttttgcag aacgccaaaa
    11101 agcgtctcta ctttcttgat gactttgaaa aaatggaagg ggttgttttg cttgctggat
    11161 aggcgaatac agcgacattt tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc
    11221 ctttttcttc aagtatacca gtacgtcata aaaaggtcat gagtctcgta ttcgactttg
    11281 tagattccat aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata caaaaatcac
    11341 aaaaattaat tgaaaatgag agtgttttca atgaaaacgt gattttgtaa tggaatttta
    11401 aaataaaaga aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa
    11461 ataagaggag gaacatgatg actgagtcag tttatgtaca tattgatacc acaagtaatg
    11521 ctgttttaac aaaagggctg actgctactg actttgcaaa cagtatcgtg cattttcctc
    11581 aaaacttgct cttgcttgat ccatctgctt ctgctggaga atatgaatcc catacagggc
    11641 tgaaagtaat ccgtggtaca gaaaatattc agcgtttctt ttcctcttct cgtaacaggc
    11701 gtggattcga tgaattgaag tggatcgact ttactgattt aacgatgctg aaagaattga
    11761 cacctttaga aatttcagaa ttattatatt ttggtcatat gaaaacacat cttcattcgc
    11821 cgttttttta taaactacag aataattttg tatacttcga tcttaatgat cagctgaatc
    11881 ggatttatta tcgctattta gatgaatttt ataggatttt tgctagtaaa ttaagttcga
    11941 tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa
    12001 aaatcagtgt ggaattattg aaagaaatgc gtaatttgat gcaggaaggg atcatttttt
    12061 cttttgatca agttggtttg atcgatggta aatatcgtat cccaatctat gtgattgaag
    12121 attatatttg gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc gctactttaa
    12181 tctactctgt caccgatcga cactggcaat tggtagaaga tgccgatctt tcttatctgc
    12241 cattgaagta gatctatttc ttatcaaaaa ataaaaagga aacaagaaac taggacatga
    12301 ttctgtctca agtttcttgc ttctttttta tttacagctc ctgtcgttta tctggcacaa
    12361 aaatcgaagg agtcgtttgt tggatcccta ataaaacgat gatcgttaca atagctgtac
    12421 tcaacccgct ggcaccattc attacgaaag aaaagagcca aggattcatc ccccatagtg
    12481 catacgctcc ccagaaaacc actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc
    12541 caatcaatgc accaaacacg atgctttttc taatttgttt cgtattatcg tctacatagc
    12601 ctttttggac atttgaagcc atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt
    12661 actcaatcaa cacttgaacg acacttaaat aataaacttg tcccaacgga aaatgtagga
    12721 tgccccaaat gaaacctgcc agcatccctg cctttgtccc tcttcttaac gcaaaaagag
    12781 tcaatggaat ctgccctaat gaaatagaaa aactgctgcc gatctggatc ggaatcaacg
    12841 acaacaccat cgctaacgct gcaacaatcg ttccttctac ccaaactcta ttttttccca
    12901 taaaaaaact tcctcctttg aataaaaaag tcacaaagga ggaagcagct ttcgaaaata
    12961 gtcaaacagc tgtttattca caattcctac gctcgcataa ccgatacagg tacgaagggt
    13021 ttagaaatag attcaatctc agcccaattg gactcccctt tgtgagttta tttattattt
    13081 attttttgtt tcaaacaatg gactaactgg tttgttttca tggatacgtt tgattgcatc
    13141 tcccattagt ccgccaacac ttacttcatc gattttgtcg attttacgat cttctggaag
    13201 atagatcgag tctgtaacaa ctagacgctc gatagctgaa tcttctattc tttgtaaagc
    13261 agggccggat aatactggat gtgtacatga agcatacaca ctagttgcac cagcttcttt
    13321 caaagcattg gcagccaatg tgatcgtacc tgctgtatcg atcatatcat cgatcagtac
    13381 acaagttttg cctttgactt gtccaatgat attcattact tctgcaacat ttgcttttgg
    13441 acggcgtttg tctatgatcg caatcggaga tttcaaaaat tccgctaatt tacgtgcccg
    13501 tgtgacacca ccatggtctg gtgaaacaac gacaacatca tcgcctttga ttcctttttc
    13561 taagaaataa ttggcaataa gtggcgcacc catcaaatga tcgactggaa tatcaaagaa
    13621 accttggatt tggactgcat gaaggtccaa agtcagcatt cttgttgcac cagctttttg
    13681 aatcatgttt gctactaatt tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg
    13741 acgcgcatag ccataataag gcatgactac gttgattgtt tttgcacttg ctcgtttcag
    13801 tgcgtcaatc atgatcagca actccatcaa gttatcattg actgggctgc tcgttgattg
    13861 aataacatag acatgcgatc cacgaatact ttcttcaatg tttacttgaa tttctccatc
    13921 actaaattga gtaacagatg attttcctaa ctcgacgccg actgctgctg caattttctc
    13981 tgctaatggg cgattggaat ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt
    14041 tgacatgggg acctccactt tctttttcac aactctagaa ttctacctaa taaatagtag
    14101 tcatattttt taaataaatc aagtgatttt gcaaaagtct tagtttagat aagggagttt
    14161 ttttgcatat ccttctttat tgacttgtct tgctctcgca attgccagcg catattctgg
    14221 aatattgtct gtaattgttg acccggcagc aatcgaactg tttttagcta cctcgacagg
    14281 tccgatgata ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt
    14341 gttctttcca tcatagttga cgaagacaac accacatccg acattgattt cttctcccag
    14401 cgtagcatca ccaacataag tcaagtgacc gactttagtt cctttgccga tttgagcatt
    14461 tttcacttcc acaaagtttc cgatatggac gttttcgcca atttcagctt ttggtcgtaa
    14521 atgtgcataa ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt
    14581 ttcaatgacg acgtgatcct caatcctact gtctacgatt tttgaatgtg acccgatgac
    14641 acaatcagaa ccaatgactg tatttccttg gatttgtaca cctgcttcga tcaatgtgtc
    14701 tggaccgatt tctacgcctg catcgatgta tgtcgttgct gaatcgacaa agcttacgcc
    14761 attgaccata tgcatctggt taattcgctt tctcatgatc tcatttgctt tagctaacgc
    14821 gatccgatca ttgactccca ttgattcttc aaaatcatcc gtttgataag cagcaactgt
    14881 atggccttct tcttttaaga tttcgatgat atctgttaga tagtattcac cttgagcatt
    14941 gtttgttcca actttgctta acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc
    15001 tgtattgatt tcttgtacaa gagcttcttc tggtgttgca tctttttgtt ccacgatttt
    15061 ttctacgatc ccaatatggt cacggatgat tcgtccatat cctgttgggt tttctgcttg
    15121 tgccgtcaag atcgttgcac ttgcattttt tccttggtga tattcaaaaa gattgttcag
    15181 cgtttcagta gtcaataatg gtgtatcgcc actgataact aacgttgttc ctttttttcc
    15241 ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa
    15301 cgcatattta ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt gtccaacgat
    15361 cgtaatcact tcatctggtt tcgtttcact gacccgatta atgatatgtt ctaccatagg
    15421 tttaccagaa acgggatgta aaactttata taattttgac ttcatgcggg tacctttccc
    15481 tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc actcctaaat atagtttgct
    15541 gatttcagtt tatcccagta tacccatttt ttcaacaaaa caagtgattt tcttttatcc
    15601 cagcgttttc tggataaaaa aatagataga ccataacggg cctatctatt tggtaaccgg
    15661 tgctctcctg ccaactcctc ggtcacaagt cacactactc ataaaacatg aaaagctgtt
    15721 ttttgaaatc gtttcttgtg acttagagtt aaacggtagg cttacaacct cttcattaaa
    15781 cgtttttcaa tcagctgacc ttcggtatta gctcaataaa cggcaaaata tgaggagcaa
    15841 tttttccgtt tattgagcta atactcagga caggacgctg atttcacaac ctctagataa
    15901 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc
    15961 cattttcaca aaatttttct tactactcga aactaagcgg ctttttcaca acctcttatt
    16021 ctgaaaaata atttcctggc acaaccgtaa tatttttcgt ttgagtatct acatctttga
    16081 cataaagcag tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa
    16141 atacagtgat gccaacaagt tctgattcga attcttcaat caagctcttc attccgttga
    16201 ctgttccgcc gcctttcatg aaatcatcca ctacaaggac acgtgaacca cgtttcaaac
    16261 ttctcttaga cagttccatc ttttcaattc tttcagaaga tccagacaca tagttgacac
    16321 ttactgttga tccttctgtt atttttgagt cccggcgtac aataacgaat ggcgcattca
    16381 gataatagga cacagcttgg gcaataggta cgccttttgt ggctacagtc attacagcat
    16441 cgattttttc accaaggtat ttagaagcaa tgattcttcc aacttgacgc agcagttcag
    16501 gttctcctaa caaatcagag agataaacat atcctcctgg aagcagacgg tcttgttctg
    16561 atagacgttc tgctagagat tcaatatatt ttttcgcctc ttcatatgaa atttcaggga
    16621 tgaacaatac accacctgca gcacccggga ttgtttccaa ggttccatac cctcgttctt
    16681 taaatgtttt cttgatgatt cccaaatctt cactgataga agatttagca gattcataac
    16741 gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg attcaataaa tactgtgtca
    16801 tgtcaactag acgttcactg cgacgtactt tcacctattc caactccttt taagaaattt
    16861 tatcgtttta cattatataa gtttttcttc aaaaattcga gcattttatg aaaaaataag
    16921 aaaatagttc ggtatttatt aaaataaaaa gaagcctata aaaaattttt cgtttttata
    16981 aggcttcttt tctttataat ttagttattt tttcttctga aggcaaccaa tattcgtttg
    17041 atgatattta caatcaggaa caaagcaata aagactaatg tgattgttgc tcctggaggt
    17101 gtatctagat aaaacgagct agttagtcct gacagcatcc ctagaaatcc tacacaaaca
    17161 ctgatagcaa tcacgacatt gaagcttttg cctaatcgca taccgattgc tgcgggaaga
    17221 accatgattg cggatatcaa aagtgcaccg gcaatcggga tcatcacagc aatagccaca
    17281 ccagtaatca cattgaacat catcgacatc aaatgaacgg gtagcccatc cacgtgggcg
    17341 gtatcttcat caaaagtcag aacatacatc ggccgtttga ataaaaagaa caatataccg
    17401 atggcaacga ataagattcc taaaaagatg acttgctccc atgtaatggt cacgatcgaa
    17461 ccaaacagat aggattggat acttgttgct gaattccctc cgctcaaatt catcaatact
    17521 aacgctagcg ccaaaccacc agccattaaa atggcaatgg agatttctga ataggttcgg
    17581 tagatcgttc gcagatactc caatatcaca gctgctaata caacgactag catggtcgta
    17641 agtgtaggat taaggttcaa aaagaatcct aacgctaccc ctgctaaaga gacatgagaa
    17701 agcgtatcag ccatcagcga ttgtcttctg atcactaaaa acacacctag catcggggcg
    17761 attccggcaa tgaagatagc ggcaagaaac gctcttctca tgaattcata tgaaagcaac
    17821 gccattgaga atcctccttt ctcaccagtc ggatctgtct gtccgcatac tgtttgatat
    17881 cttcatgatc atgggtgatc attagaactg atttcccatg gacatgcgca ctatgtcgga
    17941 gaagacgata aaattcattt ctggattctt catccatacc tgttgttggt tcatcaagaa
    18001 caaataagtc agggtcagta gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc
    18061 cagacaattc acctattcgc cgatttcgca tatcccacat ccctacagct tctaaagcac
    18121 gctgtacatg ttgatgatct ttttcagaga gacgcttgaa ccagcggcct cttggatatc
    18181 ttcctgaacg gactaattcg atcaccgtac taggaaaacc tgcattgaaa gatgcaactt
    18241 gttgaggaat ataaccgata cttaattttt ttccttcttg atttttctca gcaatcttta
    18301 tcattccttt tgaaggtttc agtaatccta acgatgcttt caccaaggtt gacttggctg
    18361 ctccattttc tccagttaaa atgacaaatt cacccgcatc tacatgataa gagacatctt
    18421 ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga tatttcgata taacgcacct
    18481 tacaacctcc attctaaaac ttaattgata ctttctttta aagctttcag atttttttcc
    18541 atgacagaaa tataattttc gcctgctttg atttgttcat tagtcaagct ttctaaagga
    18601 ttcaacactt ctagcttcac tcctgtttca ttcgacaatg tctcagctac cttcgaagag
    18661 gcattttctt caaaataaat cactttgatg ttgtttttct tcacaaattc ttttagctca
    18721 gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt
    18781 aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt
    18841 gttgcatctt ttagctcatc ggagtatttt tgatccagtg cttcaagctt tttgatataa
    18901 gcatcggcat tcttcgtaaa agtttccttt ttatctggat atttttcgcc taattcttca
    18961 gcaatcgttc tgacttcttt tatagctaat accggatcga gccaaacatg tggatcgtat
    19021 tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct
    19081 aaacggtcga ttcctttagc tgcttcgatc atcaaggtct gtttgctatc cactgaatcc
    19141 tttaacgaat ccacaaaaaa ttccatgtct gaactattat acacgaaaac atctgcatcg
    19201 gaaatttctg ctctttcttt tgcacttggt tcgaaatcat gcggctccgt tccagcaggg
    19261 atcaaaagtt tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc atacatcgga
    19321 taaaaagtcg taacgatcgt caaatcctct tttttgtctg cttccttgtt cgtatttcca
    19381 catgctgcaa acagtaaagc acctatcatt accgtagctg tcaaaagata ttttttcatt
    19441 tatatgctcc tttcaatgaa caaatcggaa ttttacgatt tattaacgtg aactaatgta
    19501 tcaaaataaa aaaacacagt caatagaaat ccatcaattt ctgatttaag catgtatatt
    19561 cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc tagccatttc actacaataa
    19621 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
    19681 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
    19741 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
    19801 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
    19861 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
    19921 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
    19981 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
    20041 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
    20101 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
    20161 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
    20221 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
    20281 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
    20341 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
    20401 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
    20461 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
    20521 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
    20581 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
    20641 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
    20701 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
    20761 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
    20821 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
    20881 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
    20941 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
    21001 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
    21061 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
    21121 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
    21181 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
    21241 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
    21301 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
    21361 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
    21421 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
    21481 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
    21541 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
    21601 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
    21661 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
    21721 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
    21781 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
    21841 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
    21901 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
    21961 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
    22021 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
    22081 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
    22141 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
    22201 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
    22261 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
    22321 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
    22381 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa
    22441 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
    22501 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
    22561 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
    22621 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
    22681 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
    22741 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
    22801 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
    22861 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
    22921 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
    22981 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
    23041 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
    23101 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
    23161 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
    23221 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
    23281 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
    23341 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
    23401 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
    23461 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
    23521 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
    23581 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
    23641 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
    23701 tagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
    23761 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
    23821 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
    23881 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
    23941 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
    24001 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
    24061 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
    24121 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
    24181 aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa
    24241 aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa
    24301 tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct
    24361 tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct
    24421 tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca
    24481 ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat
    24541 gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca
    24601 gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact
    24661 gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc
    24721 tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata
    24781 tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc
    24841 aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct
    24901 ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt
    24961 tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt
    25021 ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat
    25081 ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc
    25141 tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc
    25201 ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc
    25261 gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat
    25321 tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca
    25381 tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca
    25441 atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct
    25501 tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc
    25561 gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt actttctctc
    25621 agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct
    25681 tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat
    25741 tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga
    25801 ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata
    25861 ctatcttttt catttttatc atctatacag cttctatgat aaattattct aaactcgtca
    25921 atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta
    25981 ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat
    26041 ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt
    26101 ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa
    26161 ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac
    26221 tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt
    26281 cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa
    26341 ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt
    26401 cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa
    26461 tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca
    26521 tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct
    26581 ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca
    26641 aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa
    26701 acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt
    26761 tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat
    26821 gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga
    26881 ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt
    26941 ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat
    27001 attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta
    27061 cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt
    27121 cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc
    27181 cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt
    27241 taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg
    27301 ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat
    27361 atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc
    27421 ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag
    27481 cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga
    27541 tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag
    27601 ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt
    27661 ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt
    27721 ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt
    27781 cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct
    27841 tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat
    27901 aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc
    27961 ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat
    28021 atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc
    28081 gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa
    28141 aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg
    28201 gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc
    28261 ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt
    28321 tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat
    28381 tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg
    28441 tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca
    28501 actcttctaa acgctgctga ttatctccta caactgtttg ataataaact tcgataccta
    28561 gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa
    28621 gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata
    28681 cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg
    28741 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
    28801 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
    28861 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
    28921 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
    28981 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
    29041 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
    29101 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
    29161 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
    29221 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
    29281 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
    29341 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
    29401 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
    29461 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
    29521 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
    29581 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
    29641 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
    29701 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
    29761 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
    29821 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
    29881 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
    29941 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
    30001 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
    30061 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
    30121 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
    30181 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
    30241 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
    30301 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
    30361 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
    30421 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
    30481 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
    30541 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
    30601 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
    30661 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
    30721 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
    30781 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
    30841 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
    30901 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
    30961 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
    31021 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
    31081 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    31141 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
    31201 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
    31261 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
    31321 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
    31381 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
    31441 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
    31501 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
    31561 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
    31621 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
    31681 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
    31741 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
    31801 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
    31861 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
    31921 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
    31981 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
    32041 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
    32101 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
    32161 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
    32221 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
    32281 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
    32341 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
    32401 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
    32461 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
    32521 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
    32581 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
    32641 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
    32701 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
    32761 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
    32821 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
    32881 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
    32941 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
    33001 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
    33061 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
    33121 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
    33181 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
    33241 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
    33301 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
    33361 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
    33421 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
    33481 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
    33541 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
    33601 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
    33661 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
    33721 tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
    33781 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
    33841 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
    33901 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
    33961 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
    34021 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
    34081 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
    34141 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
    34201 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
    34261 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
    34321 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
    34381 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
    34441 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
    34501 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
    34561 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
    34621 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
    34681 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
    34741 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
    34801 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
    34861 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
    34921 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttct
    34981 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
    35041 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
    35101 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
    35161 aaagcaaacg aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa
    35221 attcgatctc aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag
    35281 aattttcttc aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc
    35341 cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga
    35401 tacgaccttc taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta
    35461 ctatcaacta taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg
    35521 caataaaaat aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt
    35581 tcaagtgttt ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat
    35641 ccattgctaa acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa
    35701 ttttagcttc tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa
    35761 gatgctgaat tgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg
    35821 tctctatttt ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct
    35881 gcttcctcaa cagagaatag atttgattgc ttataacgct tttataataa cttccaaagc
    35941 taacaccttt gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt
    36001 gttcccaatc ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa
    36061 gtggaaaata tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc
    36121 gcaataactc ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat
    36181 catcaatgat tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg
    36241 tccaattttt gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa
    36301 tcaataaaat gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc
    36361 ttatataatt cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct
    36421 gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt
    36481 ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat
    36541 ttcttataat tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc
    36601 attccttcat atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc
    36661 attttgtcct gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg
    36721 tctacaatca acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga
    36781 tacataagca aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg
    36841 atcgtcagca attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta
    36901 ccaatgacta aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata
    36961 tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc
    37021 gtcacgcgtg caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt
    37081 gtgattccaa ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct
    37141 gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag
    37201 gggtcttctt ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa
    37261 ggtgtaatcg caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt
    37321 tgtttaggaa cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct
    37381 actttttctc ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc
    37441 gcttctactg cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga
    37501 tcagaaactg actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg
    37561 tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc
    37621 aactttctga atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg
    37681 cagatagccc atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt
    37741 ttttgcgctc gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc
    37801 tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac
    37861 ataattcgtt gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg
    37921 gtcacgaaca tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa
    37981 ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat
    38041 aagatccctt tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg
    38101 cttcttgctt gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct
    38161 gctgtaaata aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc
    38221 aagacagcgg cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca
    38281 tagaccactg tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc
    38341 tgttctaaat tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca
    38401 gtttcttggc ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct
    38461 gcttctttca tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg
    38521 aagaaacgca agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc
    38581 cctagagatt tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc
    38641 cagtaattag cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga
    38701 tgaggaaatt ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt
    38761 gttgccataa cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc
    38821 caagaaatct cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt
    38881 tgctgctgtt cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg
    38941 tcacttaatt tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat
    39001 tcataagcat agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga
    39061 tccatgac
//
LOCUS       DANKHJ010000019        38259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000019 DANKHJ010000000
VERSION     DANKHJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..660)
                     /locus_tag="PW113_001122"
     CDS             complement(<1..660)
                     /locus_tag="PW113_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2525450.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KA"
     gene            802..3138
                     /locus_tag="PW113_001123"
     CDS             802..3138
                     /locus_tag="PW113_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2525451.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(3253..3564)
                     /locus_tag="PW113_001124"
     CDS             complement(3253..3564)
                     /locus_tag="PW113_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525452.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            3745..5220
                     /locus_tag="PW113_001125"
     CDS             3745..5220
                     /locus_tag="PW113_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2525453.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            5267..5851
                     /locus_tag="PW113_001126"
     CDS             5267..5851
                     /locus_tag="PW113_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2525454.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            5853..6236
                     /locus_tag="PW113_001127"
     CDS             5853..6236
                     /locus_tag="PW113_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2525455.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(6553..6837)
                     /locus_tag="PW113_001128"
     CDS             complement(6553..6837)
                     /locus_tag="PW113_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2525456.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(7189..7899)
                     /locus_tag="PW113_001129"
     CDS             complement(7189..7899)
                     /locus_tag="PW113_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2525457.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(7896..9368)
                     /locus_tag="PW113_001130"
     CDS             complement(7896..9368)
                     /locus_tag="PW113_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2525458.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            9506..9979
                     /locus_tag="PW113_001131"
     CDS             9506..9979
                     /locus_tag="PW113_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2525459.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(10132..10683)
                     /locus_tag="PW113_001132"
     CDS             complement(10132..10683)
                     /locus_tag="PW113_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2525460.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            10788..11318
                     /locus_tag="PW113_001133"
     CDS             10788..11318
                     /locus_tag="PW113_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2525461.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(11801..12244)
                     /locus_tag="PW113_001134"
     CDS             complement(11801..12244)
                     /locus_tag="PW113_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525462.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(12260..13420)
                     /locus_tag="PW113_001135"
     CDS             complement(12260..13420)
                     /locus_tag="PW113_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2525463.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13927..15627
                     /locus_tag="PW113_001136"
     CDS             13927..15627
                     /locus_tag="PW113_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525464.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(15729..16562)
                     /locus_tag="PW113_001137"
     CDS             complement(15729..16562)
                     /locus_tag="PW113_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2525465.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16866..19031)
                     /locus_tag="PW113_001138"
     CDS             complement(16866..19031)
                     /locus_tag="PW113_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2525466.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            19291..21585
                     /locus_tag="PW113_001139"
     CDS             19291..21585
                     /locus_tag="PW113_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2525467.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            21578..22243
                     /gene="pgmB"
                     /locus_tag="PW113_001140"
     CDS             21578..22243
                     /gene="pgmB"
                     /locus_tag="PW113_001140"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2525468.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            22236..23243
                     /locus_tag="PW113_001141"
     CDS             22236..23243
                     /locus_tag="PW113_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2525469.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            23265..24284
                     /locus_tag="PW113_001142"
     CDS             23265..24284
                     /locus_tag="PW113_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525470.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(24565..25932)
                     /gene="nhaC"
                     /locus_tag="PW113_001143"
     CDS             complement(24565..25932)
                     /gene="nhaC"
                     /locus_tag="PW113_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2525471.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(26041..27462)
                     /locus_tag="PW113_001144"
     CDS             complement(26041..27462)
                     /locus_tag="PW113_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2525472.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(27705..29582)
                     /gene="tdc"
                     /locus_tag="PW113_001145"
     CDS             complement(27705..29582)
                     /gene="tdc"
                     /locus_tag="PW113_001145"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2525473.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(29873..31129)
                     /locus_tag="PW113_001146"
     CDS             complement(29873..31129)
                     /locus_tag="PW113_001146"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2525474.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(31241..31446)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            31709..33010
                     /locus_tag="PW113_001147"
     CDS             31709..33010
                     /locus_tag="PW113_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2525475.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(33079..33678)
                     /locus_tag="PW113_001148"
     CDS             complement(33079..33678)
                     /locus_tag="PW113_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2525476.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            33932..34813
                     /locus_tag="PW113_001149"
     CDS             33932..34813
                     /locus_tag="PW113_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525477.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            34826..36436
                     /locus_tag="PW113_001150"
     CDS             34826..36436
                     /locus_tag="PW113_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2525478.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(36513..37172)
                     /locus_tag="PW113_001151"
     CDS             complement(36513..37172)
                     /locus_tag="PW113_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2525479.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(37455..38120)
                     /locus_tag="PW113_001152"
     CDS             complement(37455..38120)
                     /locus_tag="PW113_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2525480.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        11569 a         7771 c         6999 g        11920 t
ORIGIN      
        1 cgccttatag cctagtcccc gtactgtgat gatttgaaaa tcttccgtct tttcaaaacg
       61 ggtccttaaa cgtttgatat gtacatcgat cgttcgttct tctgtattcg tatctaatcc
      121 ccaaatatca tccatcaact gttggcgagt gaagatcttg ttgggataag acagtaattt
      181 atacagaagc agaaattctt tttgaggcag aatcaacgaa tgctgatctt gtaacactgt
      241 ataagcatct agctctaatt ttgtttgtcc gattatcaag ctactgctgt aattgatctt
      301 tgaacgtcga agcaatgctt ccacacgtag gatcatctca ttgacatcga tgggttttgt
      361 catataatca tctgctccta atttgaaccc gcgtttctta tcctcaaaat cggcctttgc
      421 cgtaatcatt agaacaggtg tgtcaaatct ggtgctacgt aatgagtcta gcaagtcata
      481 gccatccatt ttcggcatca tgatatctgt aatgattaat tctatacgtg tcgcttctag
      541 ttttttcagc gcatcctctc cattcaatgc ggtgatgact gaaaagcccg ctcgttccaa
      601 cacagaacgg taaagcaact gtaattgcgg atcatcttct actactaaaa tacgattcat
      661 tgatctccct ctgttttttg aaaaatttct agattttttc ttgtagttca tcttcagtta
      721 acatttattt cgtatactac gatcattgtt ctgatatact tattagtata ccgaatattt
      781 caatacggga gaagggtgat tatgcttcaa ttaaaagata taaaaaaata ttataaagtc
      841 ggcgaaacca ccaccaaagc attggatggt gtgtctgtcg ctttcagaca aaaagaattc
      901 gtagcgattt taggtcctag tggttctggt aaaacgacta tgctgaatgt gattggtgga
      961 ctagataatt atgattccgg tgatatggta atcaacggaa agtctactaa agactttaag
     1021 gacagtgact gggatgccta ccgaaacaac tctatcggtt tcatttttca aagttacaac
     1081 ttgattggtc atctaggcat tattgaaaac gttgaacttg ggatgacatt aagtggtgtt
     1141 tctaaagacg aaaaacgaaa acgggcagaa gatgcattgc accgagtcgg cttgacaaat
     1201 catatgcata aaaagccaaa tcagttatct ggtggacaaa tgcagcgagt ggccatcgct
     1261 cgagcactag ccaatgaccc agatatcctt ttgtgcgatg aaccaaccgg agcgttagat
     1321 acagaaacaa gtatccagat catggaattg atccaagagt tatcgaaaga aaaattagtg
     1381 atcatggtca cacataatcc tgaactggcc catcaatacg cagatcgaat cattgaattt
     1441 tctgatggta agatcttaac agattcccat cctcacatcg aacgaccaaa agatgatcaa
     1501 tttaacttac gcagaacaaa gatgagtttt tggacagcgt taaagctttc cttcaacaat
     1561 atccgaacaa aaaaaggacg tactttcttg acctcctttg cttccagtat tgggatcatc
     1621 ggaatcgcga tcgttctgtc cctttcttct ggattccaaa aacaaatcga taacacgcaa
     1681 gctgaaacga tggcgaaatt tccgattact atttctaagg taacgactaa ccagactcgt
     1741 gacgatgccg gattgggtgc aagcaaagcg gattatccag acagcaaaac gattactgca
     1801 aaagtcagcg aagaagataa agcacagcac acgaataaga ttgatcaaac atacgtcgat
     1861 tacgtaacag acatcgatcc taacttaagt aataatatcg gatttacccg tactacagga
     1921 atcaatcttt tacgtgatgt aaatggcaaa gtacagcctg tcagcttctc gaatcaaaat
     1981 cctgatacgg aatctctttc tttatctagt acgatgtcag caatgacagg tgtgggagtg
     2041 tcaagtttcc ctacacagct tgacactagc aaagaaaact ttttaaaaga caattattct
     2101 ttacttgccg gaagttatcc agcctcagca acagatgtcg tgctgatcgt agacggcaat
     2161 aataatacta atatcaacgc attgaagaat cttggatttg atgtgaaaga agacgaaaaa
     2221 cttgattttg atgatatcgt tggtacaaca ttcaaattag taaacaacaa tacttactat
     2281 acaaaacttc caactgggaa ttttatccct aatacagatt atgatgcgat gtatcagaat
     2341 gcttctgatg aattgaagat ctcggggatc ctacgcgtga aatcttcttc tacaatgaat
     2401 ctcttatctc caggaatcgc ttatagcgat caattaacga ctcaaatcgt caacgaaaac
     2461 aaagaatcag aaatcgtcaa agctcaaaga gacagtggtg taaatgtctt aactactgaa
     2521 aaagttgacg aaaatgccaa acaaacattg cttagttatt taggtggaga cagcttgcct
     2581 tcaagtatca tgatttatcc gaacaatttt gaagacaaag aaaagatctt ggattatcta
     2641 gatgattaca ataaaggcaa atcagatgaa gataaaatca tttatactga tttagctgga
     2701 acgatgactg aactgactgg cggattgatg gatgctatca cttatgtcct tattgctttt
     2761 gcagggatct ctttagtaac aagtatgatc atgatcagta tcattactta tacatctgtg
     2821 attgaacgga caaaagaaat cggtgtacta aaagctttag gtgcacgtaa aaaagacatc
     2881 actcgcgtat ttgacgcaga aacgtgtatt ttaggtattt cttctgggat attaggtgtg
     2941 tttattgcat ggctagctac gttccctatc aatagcatct tatacaacat gacggatcta
     3001 aaaaatgttg ctcaactaaa tcctgtccat gcgattattc tagtgatcgt ctctactata
     3061 ttgacgatgc ttggcggtca tcttcctgca cgaatggcag cgaaaaaaga cgccgctatc
     3121 gctttgcgtg cagaataagg ttgtgacaac cacatttaga tttgagtcat aagagcgctg
     3181 aaatagtaga aaacagcaag aaacgaggaa gtaatatgtt ccttgtttcg cgctgttttt
     3241 tttaattatt ttttataatt ctggtatact tttctttcct ttgtaccaca actcttctgg
     3301 cgtatacctg tcttttccat ttatgaatac tgttccatct gcttcaatcg ttacttcagt
     3361 cccaatccac atattacgag aaagtgcgag aagatttttt ctttttcctc ttcctttttt
     3421 tccaaattgc aaggcatcgg ttatctcttc tgtccaatga gatttcacgt attttacttc
     3481 tccgttttca aatgtaactt tcaaaatatt cgctatattt tccactttta ctgctttagg
     3541 aaatgactcc tccatttttt tcactttcct tcctccaaca ctaagtatgc gctttctttt
     3601 agtgataagt atatcatagt ttattctatt atcaaaaaaa cgcatccatt ccattgtaga
     3661 atactttacc gaacaagttg aattgttttc ctctttttca ccttttacta tagataggta
     3721 aagacaacta aggaggaatc tctcatgaca gatcaaccgt atgatttaag aaaagtcatc
     3781 gaagaactaa aaacacttcc aggtcaatat cacgaaacag atattgaaat cgatcctgaa
     3841 gcagaattat ctggtgttta ccgttatatc ggtgctggcg gtacagtaaa acgtccgact
     3901 caagaaggac cagctatgac tttcaacaat atcaaaggtt tccccggcac acgtgtgaac
     3961 atcggaacaa tggctagtcg taaacgtgta ggtgaaattt tgcaccatga ttatcgaaca
     4021 cttggtcgtt tcttgaaaga cgctgtagaa aatccaatca aacctgtgaa agtagacaaa
     4081 gcagacgctc cagcacaaga agtcgttcat ctagctaccg atgcagactt cgatattcgc
     4141 aacattttgc ctgcacctac aaatactcct gaagatgctg gtccatacat cacaatgggt
     4201 gtagtcttag gatcagatcc agaaaaaaca atgagcgatg taacgatcca tcgtatggtt
     4261 ctagaagata aagatacgat cggtatgtac atcatgcctg gcggacgcca cattggtcac
     4321 ttccaaaaac aattcgaagc cttgaataaa ccaatgccta tcactatcaa tattggttta
     4381 gatccagcaa ttgccattgg cacgactttc gaaccaccta ccactccact tggctacaat
     4441 gaactatggt gtgcaggagc tttgcgtaac gaacctattc aaatcgttga tggtatagca
     4501 gtcgatgaag tgggtattgc tcgtgctgaa ttcatcattg aagcagaaat catgccaaat
     4561 gaaacaatgc aagaagatat caacacgaac tcaggaaaag caatgccaga atttcctggc
     4621 tatactgggg atgctaatcc agctgtacaa gtagtgaaag tcaaagcagt tactcaccga
     4681 aaagacaatc caatcatgca aacaacgatt ggtcccagtg aagaacacgt atctatggcc
     4741 ggtattccta cagaagcaag tattcttgcc ttggtcgata aagctattcc aggaaaagta
     4801 gtaaatgtct acaatcctcc tgcaggtggt ggtaaattga tgaccatcat gcaaatccgt
     4861 aaagaaaacg aagcggatga aggtattcag cgtcaagcag ctattcttgc tttatcagcc
     4921 ttcaaagaat tgaagacagt cattttagtc gatgaagatg ttgatatttt cgacatgaac
     4981 gatgtagttt ggactttcaa cactcgtttc caaggagata aagatatcat cgtgatccca
     5041 ggaatgagga accatccatt ggacccatca gaattaccag aatataatcc tggctctatt
     5101 cgtttccgtg ggataagtgc aaaaactatc tttgacggta cagttccttt cgatatgaaa
     5161 gaacattttg tacgtgcaaa attccaagaa gtagaagatt gggaaaaata tttgaaataa
     5221 tgaatgatct gcaactgttt tgacatcata aaaaggagtg cggaaaatgg aacaattggc
     5281 acaacggaag aaaaaaatcg tgattggtgt aagtggcgct tcaggcacca tttatgccat
     5341 caacctagtg aagaaactaa aagaatatcc gatgatagaa acacacggaa tcatcagtcc
     5401 atgggcaaaa cagaacttaa agctggaatc cgacttatca ctaactgaat tcgaaagcct
     5461 gtttgattac cattattcaa acaaagattt aggtgctgct gtcgccagcg gatcattttt
     5521 gaccgatgga atgatcatcg taccggcaag tatgaaaaca gtcgcaggta tctctgttgg
     5581 tttaggcgac aacctgattg cacgtgctgc tgatgtaaca atgaaggagc aacgaaagtt
     5641 agtcattgtt cctcgtgaaa cgccattaaa tgccattcac ttggaaaact tgactaaatt
     5701 agcacgacta ggtgtgcaaa tcataccacc aattcctgct ttttataatc agcctcaaac
     5761 gatccaagat cttgttaatc accatacaat gaaacttttg gatcaatttg aaattgtaaa
     5821 tgagcattca aaacgttggc agggtgatta atatggaaaa agaaacgata gttgaagtaa
     5881 ccaaactagg tttcacgatc caagctgctg ttaaagtaat cggtaaagac ttattgatca
     5941 ctttaacagg cggagataca ccacatatcg gaacagtcac tacattttct cgtgatacag
     6001 agatgcaaac tgtacgtttc cctagccacg atggtcggtt ccataaagac gatgtcttag
     6061 ccgaaaaaat agctgtgatc atcaaaactg ccgttccggg aagttgtacg atcacttccg
     6121 gtatccatgt tgaccatatc tccaatgcac aaatcaacgc ctcttttccg atgtcagaag
     6181 agttaggtta caagatttta gattggttgg aaaaaataga tttccatatc aaataaccag
     6241 tttattataa agatggtgaa aaaccattat gaaagaagag gttgtgaaat cagcgttatg
     6301 acctgagtat gagagaaaaa aatggaaaaa tagcttctca tattttgccg tttttgagct
     6361 aataccgagg gacagctgat tgaacgccgt ttatcaagag gttgtgataa agccgcttcg
     6421 tttcgagtag taagaaaaat tttgtaaaca tgggtttcca catttttgca taaatttgac
     6481 ttactaccga gaaactggct tttgaacacc gtttatcaag aggttgtgac acgtcttttg
     6541 tcacaacctc ttttattttt gcttcactgt aactgctaca atatcaccga tttcttttcc
     6601 gatgttttta cgaatatctt ttcgtagtcc aaggatgtag cagactgaac catctggatt
     6661 tttcacgccc atattcacga tactgccttc atacggcaca ttgtcaaaag ttgcttggac
     6721 tttaactctt ccagcaccga attcttttct tatatcatac ggaaaaataa tatatgcccc
     6781 gcccttgccg acttcggaag catagatttt tgattcaaaa gaataaattt ccctcatttt
     6841 atttctccta ttttttacat ctagtatagc atagcaaaaa aaagaaggaa ctgtgacaaa
     6901 agtcttgtcg cagttccttt tttcgaataa acggtgttct cctgccaact cctcggtcac
     6961 aagtcacaat agtcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcag
     7021 agttaaacgg caggctcaca acctcttcct taaacggtgt tcaaaagctt gcatctacgg
     7081 cactaagccc aatcagatga aaaatatgga gtgctatttt tatctgattg ttcttactgc
     7141 ttgatgcaga gcagcttttt cacaacctcc tacatggtgt tcaaaatatt attcgccttt
     7201 tgcttctttc actaattttg cgaatgcttg ttgttggaat tcagcagcta aggctttcgt
     7261 acgttgttct tgctctgctt tgctcatttt tggattttca gtcattgctt ttaccatacc
     7321 gtcttgaaca taagcttttg cttcactttc catcgtactt tgctgtttct tcactaattc
     7381 agctaacttg gctgcttggt ccgtacttaa ttctacccat gtctctggga ttttgaatgt
     7441 attcactcgg ctgtcatatt cgtgattgtt atcagcaata ttgaaccaga atttatacat
     7501 gtcgtttttg tattcccaat aagtagtttt cgtcactttt tcagcatcgc cgtctgtctt
     7561 ttctacttta tttgtttcgt tatccgtacc agttgctttt ggtttttctt gtttttcatt
     7621 tgttcggtaa agatacactt tttcttttcc attccctaaa gctttataca aaagcatatc
     7681 tgctccttgg gaatcagcac tggaaacaat tttttctgtt tttgtttccg ttactttttc
     7741 cattccaaag tgattggaca aattagcgac gatcaaactt agtgaagcga cgaagattag
     7801 tccgaaaatc aaagataaaa aggtttgcca tgttttttta gcgaacacgt tcgttaaagc
     7861 aaaagcaagc aagctgacaa tcaaaactcc aataatcatg cattatcccc cgctttcatt
     7921 agaacaggtt cttttttctt caataaaaag gcaataataa agccaagaat cccaaagatg
     7981 atcgctacga taaacgcggc atggtaacct gacagtgtgg cattgattgc ctgatcttta
     8041 taagctaatg gcgtattatc caatacagat ttgcttggga tatttgcttt tgtcacattc
     8101 gtcaacacac tgatcaaaat agctgtacca atcgagcttg cgatttgacg gaatgtatta
     8161 tttaccgctg tcccatgact gatcaagtta tttggcaatg cattcatacc agaagtcgtt
     8221 accggcatca tgaccatgga gatcccaaac atacggatcg cataaaggat cacgatatat
     8281 aaaactggag tcgtttttgt caagaaagca aatggtgcag ttgccgctgt caaaatgaac
     8341 atccctgtga tggctaaacg tttcgcgccg tatttatcga aaatcgcacc cgtcacaggc
     8401 atcatagccc ccatgatcaa tgcacccggc aacaacatca agccagaatg gaatgctgat
     8461 tctccacgaa tattttgaat atacaatggt acgaccattt ctgcccctac catcgccata
     8521 tttgttacac cagctaaaac agctgcaatc gtgaatgtag atgatttgaa tacacgaagt
     8581 tccaagaatg gatgttccaa atgtaattga cgccatgaga acaaggcgat gaaaatcaca
     8641 ccaacaacta agaaaccaat tactttcaag cttccccagc cgtcatctcc tacactagaa
     8701 aatccgtaaa gtaaactacc gaatccaatc gttgataaaa tagcggataa aatatcaatg
     8761 ctaggatttg acaatggaat aacacttttc attaggaaga atgctaaaac caagacaaga
     8821 acaacgattg gcagtaccat accaaacaag tcacgccaat ggtagttatc aatgatataa
     8881 ccagataatg tagggcctaa agcaggagca agtccaataa cgatccctga catccccatt
     8941 gctgatcctc ttttttctgg cgggaaaata gatagcatga tattttgcaa taatggcatc
     9001 gatactccca caccgcaagc ttggatcaaa cgaccgatta gtaatgtact aaaattaggt
     9061 gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca tggcagaaag atataaattt
     9121 cttgaactga atttattgat cagccatgct gttattggga tcatgatccc gttgactagc
     9181 aagaaaccag tcgttaacca ttgcacatca gacgctgaaa tatcgaaagc tttcatcaat
     9241 gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg tacaaaatgt accaattaat
     9301 aagacaacaa ctaaaagtgt ccgattatat ggttttccgt aaatatctac gggttggttg
     9361 ttactcatat ttctccttct ttctgaattt tttctgaaac tactagaata ctttagtcct
     9421 aaattcgcta tttgtcaacc tacttgacac ataagttaat acggatataa ttaagggaag
     9481 caagaggaca aaaggagtga caaaaatggt tggggcttcc cttaaacgtt tgttggaaaa
     9541 tgatcaattg gttgttggca tcagtgaatt aagtgatatc gtaggtgtgt ctccacgtca
     9601 attaaggtat tgggaacaaa agggattcat tcgttcgatc gaagctgatg cgaattgtcc
     9661 tagaaaatat cgtttaccta cagtcataaa agtagaactg atcaaaaaat atttagatga
     9721 cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc cgccaaaaga aaatgcacca
     9781 tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa ttgatcgacg aacgctatac
     9841 gattttacaa atcggtaact tcaaagaaga gaatgctaag atgtacatca ttcatgacaa
     9901 cgagacagac gaattaactt acaagctcct tcctgtcgat gaaaaagctg actacgaaca
     9961 gttgttaaaa gaactctaaa cgccactaat ctcctaagta ccaagctttt taataagtag
    10021 atgctacata aatacttgaa aaaaggcctg tttgaacata agaatatccg aacggtgatc
    10081 tgcgtttctt tcatatagaa aggaattctc catcactgtt cggatatttt tttatttttt
    10141 tcggttactt tcttctaata catctgttag ccaatttgca tattctgttt cccgattgat
    10201 cgcatgcgct aaaatcaggt agtgtccgta attttcatgt ttttcttttt cagtaggaaa
    10261 gatcgttttt tttcgctcta atagatgaag tcgtttttct tcatgaaggt cacgctgctg
    10321 ctgaataatt tctactaatg ccggatcatt cacgtctttg acgaaataca atttcagaac
    10381 aaattcgtct cgattaaccg gtagttcatt ggtgggtgta tgaatccact ctgccaattg
    10441 ttccttccct tcttctgtta attcataaac tttcttttcc aatttttcac ctgtgatctc
    10501 tacttgatgc tgaatgattc cttgctcttc catttttgca agttcaggat aaatctgact
    10561 atgttttgct tgccaaaatt ctcctatttc atttttgaac gattgattca attcatatcc
    10621 tgtcatcttc ttcttactta ataaccctaa caaaatatat cgaagtgtat tttttcttgc
    10681 catcagtttt ccctccttga caaatcttta aatccattct atcataaatg agtaatcgaa
    10741 aacatgtagt ttattacatg ttttaattta taaggagatg cttaaacatg aaagaattta
    10801 aagaattgga agattttatt ggaactcact ttatctatac gtatgacaat ggttgggaat
    10861 atgaatggta tgccaaaaat gaccatactg ttgattaccg tatccatggc ggaatggtca
    10921 aaggccgttg ggtaaaagac caagaagctg atatcgttaa attgacggac ggcgtattca
    10981 aaatcacttg gacagagcca acagggacag acgtggcatt ggactttatg ccaaatgaaa
    11041 agaaacttca cggaatgatc tttttcccta aatgggttga agaacatcca gaaatcactg
    11101 ttactttcca aaacgaacat atcgcagaaa tggaagcagc tcgtgaaaaa tatgaaactt
    11161 atccaaaatt acttgttcct gaatttgcta cgatcactta tatgggtgat gcaggtatag
    11221 acaatgaaga tgttattagc gaagaaccat acgatggaat gccagatgat atccgtagcg
    11281 gaaaatactt cgatgaaaat tataaacgag taaaataagg ttgtgaaaga gaggttgtga
    11341 aatcagcgtt ctgacctgag tattagagaa agaaacggaa aaatagttcc tcatgttttg
    11401 ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca agaggttgtg
    11461 aaatcagcgt tatgctttga gtaataagaa cgctagttag aaaatagctt ttcatatttt
    11521 ctaggctagt gggcttatta ccgagaagca gccgtttgaa caccgtttat caagaggttg
    11581 tgaaatcagc gttttgacct gagtattaga gaaacaaatg gaaaaatagt tcctcatatt
    11641 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta tcaaagattg
    11701 taacaactgc atattcacac agttaaatta gtgacttcaa aatgaaataa tgctcccagc
    11761 aaaaaattag gctgggagca ttatttcgtt attctgttca tcaaaaatca gcgttttgat
    11821 tccatttcat cacaagttcc tcctcattta ctgcttcatc tttttgctgt gtttgaattg
    11881 taaaaccttg tgaaagatag aaatgaatag ctgattgatt tttttgatag actgtcaaag
    11941 tcagtgtttt attggtttct tttatttttt tcattagttg cgtaccgatt ctttgttgcc
    12001 ggtattccct tttcacaaag attcctgcga taaactgatc aatcacccca gcaaaaccaa
    12061 taatttcttg atctatttct gctaagtaaa tcactgcatc tggtaaagct ttcctaacag
    12121 attctacttg attctcccaa taagttgatg gaatgaattc atgagaatcc aagttagcat
    12181 gcagccaaat cgatagaaca gattccagtt ctttggcaga caattttgtt gttgtgcgga
    12241 tcattttttc tctccttact tattcattga ttttaaaata aaaaccattt ttttgaagct
    12301 cttttacaag ttttcgcccc tcaaaaaagt atatcttctc ttgttttact tttctaatac
    12361 caaaagaaac agcaacttta ggatcatcag ctgaaacgat tcgataagtt ccaccaggca
    12421 attgatccag cttgactttc ccatgatggt cgctttgtac tgaaaacggc acaccatttc
    12481 tagtaagtgt ttttttctta tgtttcaaaa tgaatgaact accatttacc ggtttccctg
    12541 acgaatcact aataataaat gaaaaatcaa gcactttttt acttaatcta tactgataga
    12601 ttagtcgtcc gataaacact aacagtatga ctacagctat tatcgttcct gcaagaataa
    12661 accattgccg catggttctt tcctgatttc cttttccttc ttcttttttt accgattcgt
    12721 tataaggaat ccgatgtcct gtcactagca aacgatgcgt gttgatcata tacggcgtac
    12781 atgtaagcaa ggtaactaag tcttctcctt ctgaaatcgt tagaacagac gtgtcatctg
    12841 gcagtaccac ttgtatattt ttaacttgat aagccaattt ctcccccaaa acagtcaaaa
    12901 taaacgtgtc tccgttttct aaacgatcta attcggtaaa taaccgtcga ttaggtaaac
    12961 cgctgtgagc agagatcact gaatgcgtcg aatttccacc aagcgggaaa gaggttcctt
    13021 gcaagacgcc tgctccattt tcaaggatcc taccattgag ccgatcataa agtgaaatat
    13081 ttaattggat cttaggtata ttgatacttc cgatcagcca ttcctctaat tggctggatg
    13141 ttgtactttc atttctgttc ttttcattga aaggatcagt accaggattg aatccttggt
    13201 cagttaatcg ttggtttttc tcctccattt tctttcgctg cacttcaatt tcagctgcgg
    13261 tccgtttttg tacttgttcg atacgttttt gatcaataaa actatttaaa gaatccacat
    13321 aaaatggata taaagcgatc agtaacccta cgacgataca tattaataaa aacaaacgaa
    13381 acaaaagtcg acgagactgt ttttgcttac tctgcttcac atgtgtcctc ccttgcaagt
    13441 ttttttgttc tttcttttat tatatgggtt caggaaaaaa atagaaaatg aaagccgatg
    13501 aggttgtgaa aaagctgtcc tgcatcaagt aataagtaca gcccaaaaaa atagctactc
    13561 atattttttg ttgggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg
    13621 aagaggttgt gaaaactgcg ttatgctttg agtaataaga gcgctagtca gaaaatagct
    13681 tctcatattt tctaggctag tgagcttatt accgagaagc agctgattga acaccgttta
    13741 ccgagaggtc atgaaaaagc tactttgttt cgagtattaa gaaaaattct gtttcaaaac
    13801 aaggagatgc aacaaaactt atgtcgcatc ctctctcctt agaactttca cttatgtttt
    13861 tcttgtaaaa ttcataaata ttttcatgaa ttttctaatt tcttgcctac ctctcgcata
    13921 ggatatatga tgaatttatc taaagtttgc aaaggagttc gacaattgaa aaaatatggc
    13981 ccatttttag caattttttt cattgctttg gcatttctca tgcctcattt attcacacat
    14041 aaactaattt tgggtgctga tgccccattc cattacaatc ggttctatga tgccgcgcaa
    14101 caaattaaat atggggattt ccattatttt ccttcccttt atggattcca gcaaagtggc
    14161 aggatcgtga atgccgttta cggcccattt ttcgcttatt tacaaggatt gatcgtgttg
    14221 cttgcaggta gctggtttca ttatcagatt ctttcaaatt tcattcttta tcttatttca
    14281 ggattttcta tgctgatttt actgagatat gtcaatgttc gaaattgggt aagtgtacca
    14341 gtagccgttt tattcatgag tacgtattct attcaatact ggtctatcaa ccaaggattc
    14401 actagctggg ggacttgttt ttatccttta tgtatgatcc ctattgctga tttcgtcctt
    14461 cataaaaagt tccctgttat caaagtcgct gtctcagtgg gacttatgac acagatccat
    14521 ttactgtctt cgatcatgtt agctttgatg tatatccctt tttatcttta ttactttttt
    14581 aaccagactg aaaaaggaaa agcactgctt caactgatta agtcggtctt gctttatttt
    14641 gttctgacag caaatatttg ggcaggatta gctattgttt acagtgggaa taagattctt
    14701 cctccctttg ttaacagaga aatgagtcaa aaggcgatag atctggatgg cagttattgg
    14761 ctccattacc ctaaggcctt tcctatattg atattatcag gttttttgct ttttattttc
    14821 tttcaacaaa agaacacctt attccaaaaa atcgtgttgt acacaagtct tgttttcttt
    14881 tttctttcta ctagcttgat tccatggacg acattgatcg aaaaaaatgt gccttttatt
    14941 agtttgatcc agtttccatt tcgatttttc gtgccattta cgctattgct tctactttac
    15001 cttgcccttt cactgaacca gtggtctgaa caaaagtggt tccgtctttt aagtatattt
    15061 ggcattctta ttttttcagc acagacgatc cagaacttat accagcatct ggaaaagtgg
    15121 gaaaatgaaa catttgtcag tcgacatact tatctattcg atacgccgga agaagcaagg
    15181 aaaacattct tcagcaaaga tatgtctacc agcttgttcg ttttccaaaa aaccacacct
    15241 gactatctgc caatctataa ggagacaaaa gacaataaat acgaccggta taacgaagaa
    15301 atactagaaa acgaaacaac ttttataaaa acacatccag gcggtctttt gacaatcaag
    15361 tggaaaaaca ctggggacaa aactgttcat atccctgtta tcgtctacga tcggactgtg
    15421 ctacagcaaa atggaaaaac attgactgat tatgaagtaa cagatatcgg gaccccaatc
    15481 gtgaagcagc aaaaaggaat caacgagctc acgcttcatt atcaaacgcc aatctatttc
    15541 tatttcattc tttctcttac tttgattggc tggtttacct tgctatgttt atttatttat
    15601 catagataca agcttttacg tgcttaggca aaagggataa atacatccga tcaaaaaact
    15661 ctccaaacgg tataatcgtt tggagagttt tattttaatc agttttttta tgttatatgt
    15721 tatatttttt attcgttcac atctaattcg atttccaccc cgtagtgatc actgatgatt
    15781 ggactatcga tcccatcaaa aatccgttga tattttttaa cctgcattcc ttcagggacg
    15841 aaaataaaat ctatacgcaa tttttctgta ttttgttccc aaccatctat tttcttttcc
    15901 acagtagctg cttttccttt ttcattcgcg acaacatacg aatcttgaag aagaaggcaa
    15961 ctttctctta cagtataata accttgagag tctactggat tattgaaatc acctaggaaa
    16021 aaaagtggta cgcgagtttc caaaaagtag ttttccaagt ttttccactc aaaataaaag
    16081 ccactttttt tatctgtcca ccaagaaaag tggcagctcg agacaaaaat gtcctgatca
    16141 tctatctttg ttttcccaat caagatcttg cgtgtctgat aatcttcttt cttttgaaat
    16201 tgggagacgg taaggacctg actttccaat gtttttttcg taagcagtgc atttccttct
    16261 tcatatttat cataaccgat gtggctcatt tcccaactcc aaaaatactc ttttccgtgt
    16321 tcctttaaaa gctgaactaa acggtaggca aaattatctt tacggatagg tacttgattc
    16381 tttaccggac agaacttcat tagcttcgca ggttctactt cttcagattc cagtaattgg
    16441 ttgatttctt gtaaagcaat cacttcataa tcttctaaca ggatcttatc tgctaattct
    16501 tgcaattttt tctctggttc tggctcaagc cagctatgcg tgttgagtgt catgaatttc
    16561 attggttttc tccttgtctt catttcgaaa caaaagggtt gtgacaaaat ttttgtcaca
    16621 acctctccag caaatagtgt tctcctgcta actcctcagt aataagctca atggaccagg
    16681 agctattttt tgtttggctg tttttatcac ttggtgcagg gcagcttttt cacgacctct
    16741 ccactaaaca gtattcaaac tgctgcttct cggtaataag cccaaccaaa cgaaaaatag
    16801 gaggagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    16861 tctccttaca ttttaatgga gccgatcact ttaccagctg tcactgtacc taattgcttg
    16921 atcactactt cttctacttt atcgtttgta aatacgatca tgatcgttgg atctttttct
    16981 tcgttttgga tcacatcaag atccatctca gctaatttcg ttcctgttcc tacttcttgg
    17041 ccttctttga cagcaatctc aaaagctggc tgattcattt ggactgtatc gattcccata
    17101 tggatcaaaa cttcgatacc atctttcgtt tccagcccaa ttgcatgttt gctagggaaa
    17161 atacttgtca cttttccagc gatcggcgca acaacttctc ttgatgttgg aataacagca
    17221 aagccgtcac ccatcatttt ttgtgagaat accggatctt ttacttcaga taaaggaatc
    17281 acttctccag aagctgcgga aactagttct tgttctatac ctaagtattg cttactacct
    17341 gcttgtccat ttcctgtaga aaccatttct ggctctggaa tctctgctcc tgattgcagc
    17401 agatcttcga tatctgattt caagacatct gcttttggtc cataaactgc ttggactcca
    17461 ttgtctttga cgatcaatcc catcgcccct gctcgtttcc aggcttcttc agtccctact
    17521 ttttctcgat catttacact tacgcgtaac cgagtcatac aagcatcgac atctttgata
    17581 ttttcttttc ctcccagcaa atgaatgatc tggaccactt gctgatctgc tgttcctgtt
    17641 ccgctgttag atacaacggt ttcttcactg tcattgtcgt aatttccgtt acgtcccggt
    17701 gtagcaaagt tgaatttttt gatcaagaaa tttgctaaga aatacgtaac aacaccaaaa
    17761 gcgatcacac acaacacgaa gttgataaga tctccaccta gacccgcttt gatagctaac
    17821 ggtgtccgag tcagcaattc gatattccca aatgaatgga cccgcaatgg caagatatct
    17881 gccatcgcaa atgcagcccc ttggatcact gaataaatga catataatgg cacagcagca
    17941 aacataaaca tgaactccaa tggttctgtt acacctgtta gaaagacagc caacgctgct
    18001 gaaaaataca ttgatttata tcttgctttc ttatcaggat cgacattacg atacatcgca
    18061 aaagccattc ccatcaagat acctgaagaa ccaatcattt gtcctacttt gaaacgagcc
    18121 ggcgtccagt tatgcaacac aaactcatat tgcgacatat cccctgcacc tttaagattc
    18181 actaaatcag tggcccaagc caaccataac ggatcttgcc caaatacttg tgtccccgcc
    18241 tgtgcgcctg ataaaatctc atacgatccg ccaagctgtg tatagttgat aggaatcgtc
    18301 agcatatgat gcaatccaaa tggcaatagt aaacgttcta atgttccaaa taaaaacggt
    18361 gctagaatcg gtgcgctttc ttgtgattct gcaatccata aaccaaaatt attgattcca
    18421 gcctgtacgt ttggccacac cacagcaaga atcaatgcca cgattgttga ccataagata
    18481 acaacaaacg gaacaaagcg ctttccattg aaaaatgaca aagcatccgg caattttcta
    18541 taattatagt acttattata agccattgcc ccgacaaatc cagcgatgat ccctacgaat
    18601 actcccatat tcaaagctgg tgcttccaat acacttgtaa aaaatccttt aaccatgatc
    18661 ttcgtaccga acagagtgtg ggtaaatgca tcttcattcg caagcatgtc acttgtgaca
    18721 ccaaagatag ccccagtgat ccgattgatt aaaataaatg agatcccagc tgcaaatgct
    18781 ccgcctgctc tttcttttgc ccaacttcct cctattgcta aagcaaataa aatatgaaga
    18841 ttaccaataa ttgcccaacc aatattttca attactccgc ccgttgtgac aagcggcgtc
    18901 caatcaggat tgatcaaggg aattgatttc cctatactga tcatcaaccc agctgccggc
    18961 ataactgcta ccacaaccat taaagccttt ccaaattttt gccaaaactc aaaactaaat
    19021 atctttttca ttccgtttag ctcctttacg caatcggttt cgttaaacgt attatagcgt
    19081 gcgttttctt ttttgtaaat gctttatttt ttatttttct gtctatacca ttctataaaa
    19141 cctataatac gtgtgttccc ttctataatt ttctcctaaa aatttatttt tttaagcttt
    19201 tttctatgaa atattttctt ttgtctattg acttgtgcaa tcggttgcgt ttattatgaa
    19261 cgtataaaac agctcattag gaggagaaaa atgaaacaga tcaaacgact atttgctatt
    19321 gatccatgga aaatcagcac gacacaatta gataaaaaaa atcttcgact gcaagaatct
    19381 ttaacaagta ttggaaacgg ttatatgggg atgcgcggga actttgaaga aacttactca
    19441 ggggaccacc atcaaggtac ttatttggcg ggtgtctggt atcccgataa gacacgtgtc
    19501 ggctggtgga aaaatggtta tccagaatac tttggaaaag tgatcaacgc cttgaacttt
    19561 atcgcgatag atatttcgat caacgatcag ctgatcgact tggcttcttt ggaaccagaa
    19621 gacttttact gggaattaga tatgaaaaat ggtgtattat ctcgtacatt cacaatcact
    19681 acttctacta ataaagtacg catttccttc gaacgttttc tgagcattgt caaaaaagaa
    19741 tcagcttata tctatttaac tatagaaatg cttgaaggta gcggaacagt caaactcgta
    19801 tcaaaattgg atggaaatgt tcaaaacgaa gacagcaact atgatgaaca tttttggaat
    19861 gagattgatc gtggaactat agaagaaatc ggctatttga cgactaaaac tattccaaac
    19921 aactttggga ttgaacaatt tactgttact gcagcaatgc gccatttcgt taatggaaaa
    19981 tcagtcatcc ctgactatca cgaagatact ttagcattac atggttgctt gtctgcagaa
    20041 ttatctcaag gtgagacatg gtcagtggaa aaagaaatta tcgttcttac tagtagagat
    20101 attccagaag cgcagcaagc agaaaaagct gtctcagaat taacgaacca gacaaacaat
    20161 tatatccaag caaaagaaga gcaaacagcg gcttgggcag aacgatggaa gcttgccgat
    20221 gtggtcatcg aaggagacga agaagcacaa caaggtattc gattcaattt gttccaacta
    20281 ttctctactt actacggcga agatgaacga ttgaatatcg gccccaaagg attcacagga
    20341 gaaaaatacg gcggagccac ttactgggat acagaagcgt atgctgtccc tctttatctt
    20401 gctttagcca aacctgaagt aactaagaat ctgctgaaat atcggcataa tcaactgccg
    20461 caagcaatcc acaacgctca gcaacaagga ttaaaaggcg cgctttatcc tatggtcacc
    20521 ttcactggtg tcgaatgcca taacgagtgg gaaatcacat tcgaggagat tcaccgaaat
    20581 ggcgcgattg cctatgcaat ctacaattat acgaattaca ccggcgatac ggaatatttg
    20641 aaaaatgaag gactggaagt tttagcagaa atcgcacgct tctgggcaga ccgtgtccat
    20701 ttttcaaaac gagcaaataa atacatgatc cacggtgtga ccggcccaaa cgaatatgag
    20761 aacaatatca ataacaattg gtatacaaat acgatcgcag cttgggtctt gcgttacacg
    20821 cgagaaaatt atttaactta tcaaaaagaa gctgctatca ctatcagcga agaagaacta
    20881 gccaaatgga aagatatcgc agagaacatg tacttcccag ttgacgaaga attaggaatc
    20941 tttgtccagc atgacacatt cttagataaa gatctcatgc ctgtatccga tattcccaca
    21001 agcgatcttc ctctgaatca gcattggtct tgggacaaaa tccttagatc ttgcttcatc
    21061 aagcaggcag atgtactaca ggggatttat ttcttcaatg atacttttac aaaagaagaa
    21121 aagcaaaaaa actttgattt ctatgaacca atgactgtcc atgagtcttc cctttcgcca
    21181 agtatccatt cgattttagc agcagaatta ggaatggaaa gcaaagctgt agaaatgtac
    21241 cagcgaacag ctcgcttgga tttggataat tacaataacg acacagaaga cggcttgcac
    21301 atcacttcca tgactggtag ctggttgtcg attgtccaag gatttgctca aatgaaaact
    21361 gcccatgatc agttgagatt cgcaccattt ctgccaaaaa cttggaaagc ttattctttc
    21421 catatcaatt atcgcggacg tttattgttc atctcagtca cagatgaaca agttactttc
    21481 gagttattag aaggttctcc tctagaaatt tacgtctatg aacagtctta ttctttagcg
    21541 gatcgtctag aaataacgct aagaaaggag gaagcaaatg tttaaagggg tattgtttga
    21601 tttagacggt gtcattaccg atactgctga attccattat cgtgcttggc gaaagcttgg
    21661 tcaggaaatc ggcattacga tcgaccacac attcaacgaa cagctaaaag gcgtcagccg
    21721 agaagactca ctctctttgc ttttagctta tggcggaaaa gaacactctt tttcaaagga
    21781 agaatttgca gaattagcga aaagaaaaaa cgattattat ttagagatga tccagacgat
    21841 cgaaccaaaa gatgtcttcc ccggggtcgt gcctttactg gattctttaa aagaagaaaa
    21901 aatcaagatt gctcttgctt ctgcaagtaa aaacgggcca ttcctacttg aaaaaatggg
    21961 tcttacacct tactttgatg ctattgccga cccggcagaa gcaacaaacg gaaaacctgc
    22021 gccagatatc ttcctccttg cagcaaaagc tgtcggacta aaagcagaag aatgtatcgg
    22081 gatcgaagac gcgcaagccg gtatccaagc catcctctca agtggcgctc aaccaattgg
    22141 cgtgggacgt gctgaagacc ttggagagga gatcccgctt gtccctgata cgacttttct
    22201 tacgatcgat tacttgaaag aaaagtggca tgaccatgga taatccagag atctccgtcg
    22261 taccagttcc agagactgaa ataaagaaaa tcacattgaa gaacgactac ttagaagtcg
    22321 ttcttctcaa ttacggtgcc cgcctgcacc aaatcttcgc accagacaaa gaaggaaaat
    22381 cagaaaatgt tttattgtct tacgatcgtt tcgaagatgt tctaacagac aaaagctttt
    22441 ttggagcaac tgtcggacct gtagccggaa ggatccggga tgcttcttgg ggtgagcata
    22501 gattggagaa aaactgcgga tcacaccata tccacggtgg cacgaaaggc tggtctttcc
    22561 aattttggga agtagaagtt ttcaaaactc catcatcaat aggtgtggtc ttttacttaa
    22621 aagatgaatt ttctacctat ccaggaccaa tcactgctac gatcacttac cgtttaacaa
    22681 aaaacgaatt agagatgaca acctctgcta gtagccttgt tgaaaccatc tgcaatccga
    22741 ccaaccatgc ctacttcaac ctttcaggaa atggaacaag agacatctat gaacatcagt
    22801 taacggtctt tttagatggt gtgcttgaat tagatcaaga aaaactgcct actggaaatt
    22861 ggacaaaaaa agaaaaactg ccaatcgact ttagaaaatc cccaacactg caagagatcc
    22921 ttgcatgtta tccagatgga ctagatgatg tatttctttt gcatcatcct aggctttcca
    22981 aaacttcctt acaattattc gagaagcatt ctggtcgcca aatgactata gcaacttcta
    23041 ataaaagtat ggtattattt tctacgactg gattcgaagc tgatttctct gtcaatggaa
    23101 aacagatgca ttcaaactac ggattagcaa tcgaaccgca agaaatccct gatatcgttc
    23161 atttccccaa atttggatcg atcaacctcc atcctggtca agaacggatc agtcagacga
    23221 tttatcggtt ctctgcacaa taacaagaga aaggtgtttt tatcatggct attaccgtaa
    23281 aagatgtggc gaaaaaagcc ggtgttgcca cctcgaccgt ctctcgtgtc atcaacgatc
    23341 atcctagtat ttccgagaca acaaagaaga aagtccgaaa aattatggat gaactaggtt
    23401 atgttcctaa catgactgcc cgcaatctag gaaaacgcat ttccagtgcc atcggcgtaa
    23461 tcttaccccc tcttgattca aaagagcgga tcggtaaccc attttatctt gagatcatgg
    23521 aagcaatcaa cgaagaagcg cggaattatg atatgactac agcaatcgct actgctaaaa
    23581 attttgaatt gctgttagag aacgtccagc gtatgcattt acaaaaacaa gtagatggtt
    23641 ttattctagt gtattcagat agtcaagata ctgtcattga ttatttatac caaaaccaca
    23701 tccctttcac tttgatcgga cagccttatc atcatgagaa cgaaatcgtt tatgtagata
    23761 atgataacca gcttctaggt aaacaagcaa cagaattttt gatccagaat ggacataaga
    23821 atatcctgtt tgtgacaaac acgactcatg aaagtttgta tttcgaacga tatttcggtt
    23881 atcaaaaagc aatgatgatg gctggtcttc ctgcgttatc cagtgtagta ctagagcatc
    23941 cggaggatta tctgtccttt gagaaaacgt tgaaagaaaa cgaagccaca gcattggtgg
    24001 tcattgacga tatcttcgcc cttcgcacga tgcagttggc gcaaatgtat ggctatgtca
    24061 ttccagagac actctccgtc attagcttca acaactcgat cttctcaaca ttaacacacc
    24121 cttaccttac aagtatcgat atcgatatct ccgaattagg acgtgtcgct atgcaaaagc
    24181 tgcgcgaatt gatccatgaa gaattgagca gtggcgtacg tttggtcatc cctcatcgct
    24241 tgatcaaaag agagacagtc gttcctttag aaaacccata ctaaatagct gactcactaa
    24301 atacgctgac catttatcct atggtcagct ttttatgtaa aagaagatac tacttctaac
    24361 acattgttta tctgtaaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    24421 tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
    24481 caaaaattta aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct
    24541 gtcccagcct cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc
    24601 gcctaatatt gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa
    24661 cgcgctaact ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga
    24721 attgacggtc gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac
    24781 gtcatcaaaa gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc
    24841 aacgatcaga ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg
    24901 gtccatcttt tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa
    24961 cccacctaat gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc
    25021 gcctcgagaa agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat
    25081 caagatgtct gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag
    25141 agcagctgtc gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc
    25201 aaataaaata actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt
    25261 ttggatcatt tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt
    25321 ataaaaaatc aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg
    25381 ttcgaataga ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga
    25441 gcagttattc cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa
    25501 tccaagtaaa tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga
    25561 actgccgaca gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg
    25621 cacagaaaca agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca
    25681 tgaagcaact aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat
    25741 tcctttgatg atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa
    25801 caacaaacac atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat
    25861 cagtactcct aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat
    25921 atttttattc atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat
    25981 tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt
    26041 ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt
    26101 gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact
    26161 caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca
    26221 gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc
    26281 tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata
    26341 aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc
    26401 ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac
    26461 gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac
    26521 ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat
    26581 aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc
    26641 aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc
    26701 tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc
    26761 ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat
    26821 cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc
    26881 agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt
    26941 gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca
    27001 tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat
    27061 ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt
    27121 accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa
    27181 catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt
    27241 tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat
    27301 tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc
    27361 gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat
    27421 aaaggtccca agacccattt tacttacttc agatttactc accgttgaca ctctccttga
    27481 ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag
    27541 cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc
    27601 aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca
    27661 gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt
    27721 gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt
    27781 tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc
    27841 aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca
    27901 aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac
    27961 tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat
    28021 ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg
    28081 aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag
    28141 tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca
    28201 tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc
    28261 cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt
    28321 tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg
    28381 ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct
    28441 gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct
    28501 gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg
    28561 tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag
    28621 aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg
    28681 attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca
    28741 gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca
    28801 actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat
    28861 gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta
    28921 cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg
    28981 atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt
    29041 gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct
    29101 aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat
    29161 tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag
    29221 gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat
    29281 gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta
    29341 cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa
    29401 ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga
    29461 cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc
    29521 tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc
    29581 atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc
    29641 ttctattctg taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt
    29701 atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa
    29761 ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa
    29821 ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa
    29881 ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga
    29941 attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta
    30001 tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa
    30061 caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga
    30121 atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg
    30181 ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg
    30241 ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt
    30301 cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat
    30361 cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa
    30421 cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg
    30481 ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg
    30541 tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca
    30601 gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta
    30661 cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac
    30721 cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa
    30781 atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt
    30841 tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc
    30901 cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc
    30961 ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa
    31021 cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg
    31081 tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct
    31141 ccgtgtagtt tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca
    31201 ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg
    31261 gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg
    31321 cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt
    31381 gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt
    31441 cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa
    31501 ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt
    31561 ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga
    31621 gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa
    31681 aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc
    31741 cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc
    31801 tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac
    31861 agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga
    31921 ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct
    31981 tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat
    32041 ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca
    32101 ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca
    32161 actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca
    32221 aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc
    32281 acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt
    32341 cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg
    32401 tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta
    32461 tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac
    32521 atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa
    32581 agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt
    32641 gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga
    32701 agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag
    32761 cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt
    32821 cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt
    32881 ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa
    32941 aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga
    33001 atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag
    33061 tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt
    33121 ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac
    33181 cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc
    33241 tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc
    33301 catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata
    33361 aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt
    33421 ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat
    33481 aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt
    33541 cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat
    33601 gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct
    33661 cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata
    33721 gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca agctagcaat
    33781 tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat
    33841 aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt
    33901 atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt
    33961 gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg
    34021 tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact
    34081 tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga
    34141 agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg
    34201 aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc
    34261 tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa
    34321 ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga
    34381 tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca
    34441 agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct
    34501 cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga
    34561 agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga
    34621 agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca
    34681 cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa
    34741 attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca
    34801 ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt
    34861 ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc
    34921 tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg
    34981 attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc
    35041 gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag
    35101 tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga
    35161 acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc
    35221 tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat
    35281 gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt
    35341 aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag
    35401 ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc
    35461 aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt
    35521 gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct
    35581 aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat
    35641 ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt
    35701 attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc
    35761 ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa
    35821 acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt
    35881 agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga
    35941 agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta
    36001 ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg
    36061 tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat
    36121 gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg
    36181 ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc
    36241 ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc
    36301 tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac
    36361 gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga
    36421 cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc
    36481 aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg ctctttatca
    36541 atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat
    36601 tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt
    36661 ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata gagagcgact
    36721 cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc
    36781 tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag
    36841 aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac
    36901 tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt
    36961 aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg
    37021 atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga
    37081 tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga
    37141 acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt
    37201 ctaataaaga aacataactt cccttattcc aactgataat tttcttttat catagcataa
    37261 acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt
    37321 ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt
    37381 gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc
    37441 ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt
    37501 tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat
    37561 agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt
    37621 gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc
    37681 catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac
    37741 tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa
    37801 tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga
    37861 gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt
    37921 tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga
    37981 atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct
    38041 gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa
    38101 tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa
    38161 ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac
    38221 agccaaacaa aaaactgcga tgagcatcac cgcagtttt
//
LOCUS       DANKHJ010000020        38140 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000020 DANKHJ010000000
VERSION     DANKHJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(451..1761)
                     /locus_tag="PW113_001153"
     CDS             complement(451..1761)
                     /locus_tag="PW113_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2525481.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="PW113_001154"
     CDS             complement(1773..3191)
                     /locus_tag="PW113_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2525482.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="PW113_001155"
     CDS             complement(3431..4999)
                     /locus_tag="PW113_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2525483.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="PW113_001156"
     CDS             complement(5003..5413)
                     /locus_tag="PW113_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2525484.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="PW113_001157"
     CDS             complement(5410..6333)
                     /locus_tag="PW113_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2525485.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="PW113_001158"
     CDS             complement(6330..7208)
                     /locus_tag="PW113_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2525486.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="PW113_001159"
     CDS             complement(7195..8532)
                     /locus_tag="PW113_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2525487.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="PW113_001160"
     CDS             complement(8535..9638)
                     /locus_tag="PW113_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2525488.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="PW113_001161"
     CDS             complement(9712..11220)
                     /locus_tag="PW113_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2525489.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="PW113_001162"
     CDS             complement(11186..11878)
                     /locus_tag="PW113_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2525490.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="PW113_001163"
     CDS             complement(11900..12874)
                     /locus_tag="PW113_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525491.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="PW113_001164"
     CDS             13174..14046
                     /locus_tag="PW113_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525492.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="PW113_001165"
     CDS             complement(14504..16702)
                     /locus_tag="PW113_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525493.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="PW113_001166"
     CDS             16934..17926
                     /locus_tag="PW113_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525494.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="PW113_001167"
     CDS             17986..19407
                     /locus_tag="PW113_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2525495.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="PW113_001168"
     CDS             19400..20899
                     /locus_tag="PW113_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2525496.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="PW113_001169"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="PW113_001169"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2525497.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="PW113_001170"
     CDS             22491..23174
                     /locus_tag="PW113_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2525498.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="PW113_001171"
     CDS             23176..24012
                     /locus_tag="PW113_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2525499.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="PW113_001172"
     CDS             24023..25777
                     /locus_tag="PW113_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525500.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="PW113_001173"
     CDS             complement(26053..26394)
                     /locus_tag="PW113_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2525501.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="PW113_001174"
     CDS             complement(26462..28633)
                     /locus_tag="PW113_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2525502.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="PW113_001175"
     CDS             28802..29701
                     /locus_tag="PW113_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2525503.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="PW113_001176"
     CDS             complement(29772..30629)
                     /locus_tag="PW113_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2525504.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="PW113_001177"
     CDS             complement(30614..33316)
                     /locus_tag="PW113_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2525505.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="PW113_001178"
     CDS             complement(33329..34618)
                     /locus_tag="PW113_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2525506.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="PW113_001179"
     CDS             34768..35814
                     /locus_tag="PW113_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525507.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="PW113_001180"
     CDS             35816..37969
                     /locus_tag="PW113_001180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2525508.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11255 a         7846 c         6778 g        12261 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcagggc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagcatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taaaccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg actcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga cagagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa
//
LOCUS       DANKHJ010000021        37822 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000021 DANKHJ010000000
VERSION     DANKHJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..653
                     /locus_tag="PW113_001181"
     CDS             <1..653
                     /locus_tag="PW113_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525509.1"
                     /translation="NTDEKEALTKERIQEAKNSNTGLEKLVKENLQNSTSSQGGRSVD
                     VRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDVRENVQNSTSSQGFKSMDVREN
                     VQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRK
                     SAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLLKK"
     gene            668..886
                     /locus_tag="PW113_001182"
     CDS             668..886
                     /locus_tag="PW113_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525510.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            2405..2719
                     /locus_tag="PW113_001183"
     CDS             2405..2719
                     /locus_tag="PW113_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525511.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(2772..3545)
                     /locus_tag="PW113_001184"
     CDS             complement(2772..3545)
                     /locus_tag="PW113_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525512.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            3650..3895
                     /locus_tag="PW113_001185"
     CDS             3650..3895
                     /locus_tag="PW113_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525513.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            4051..4902
                     /locus_tag="PW113_001186"
     CDS             4051..4902
                     /locus_tag="PW113_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2525514.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            4935..5609
                     /locus_tag="PW113_001187"
                     /pseudo
     CDS             4935..5609
                     /locus_tag="PW113_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            5866..6054
                     /locus_tag="PW113_001188"
                     /pseudo
     CDS             5866..6054
                     /locus_tag="PW113_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6100..7065
                     /locus_tag="PW113_001189"
     CDS             6100..7065
                     /locus_tag="PW113_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525515.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            7087..7299
                     /locus_tag="PW113_001190"
     CDS             7087..7299
                     /locus_tag="PW113_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2525516.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            7374..7850
                     /locus_tag="PW113_001191"
     CDS             7374..7850
                     /locus_tag="PW113_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2525517.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            7847..9655
                     /locus_tag="PW113_001192"
     CDS             7847..9655
                     /locus_tag="PW113_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2525518.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            9732..9947
                     /locus_tag="PW113_001193"
     CDS             9732..9947
                     /locus_tag="PW113_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL2525519.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            10039..10902
                     /locus_tag="PW113_001194"
     CDS             10039..10902
                     /locus_tag="PW113_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525520.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            10939..11475
                     /locus_tag="PW113_001195"
     CDS             10939..11475
                     /locus_tag="PW113_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2525521.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            11489..11911
                     /locus_tag="PW113_001196"
     CDS             11489..11911
                     /locus_tag="PW113_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2525522.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            11841..14240
                     /locus_tag="PW113_001197"
     CDS             11841..14240
                     /locus_tag="PW113_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2525523.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            14272..16263
                     /locus_tag="PW113_001198"
     CDS             14272..16263
                     /locus_tag="PW113_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2525524.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            16286..16537
                     /locus_tag="PW113_001199"
     CDS             16286..16537
                     /locus_tag="PW113_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL2525525.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            16527..17756
                     /locus_tag="PW113_001200"
     CDS             16527..17756
                     /locus_tag="PW113_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL2525526.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            17753..19834
                     /locus_tag="PW113_001201"
     CDS             17753..19834
                     /locus_tag="PW113_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2525527.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            19983..23903
                     /locus_tag="PW113_001202"
     CDS             19983..23903
                     /locus_tag="PW113_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL2525528.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            23904..24848
                     /locus_tag="PW113_001203"
     CDS             23904..24848
                     /locus_tag="PW113_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525529.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            24852..25055
                     /locus_tag="PW113_001204"
     CDS             24852..25055
                     /locus_tag="PW113_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL2525530.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(25355..25591)
                     /locus_tag="PW113_001205"
     CDS             complement(25355..25591)
                     /locus_tag="PW113_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525531.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(25774..26160)
                     /locus_tag="PW113_001206"
     CDS             complement(25774..26160)
                     /locus_tag="PW113_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL2525532.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(26142..27470)
                     /locus_tag="PW113_001207"
     CDS             complement(26142..27470)
                     /locus_tag="PW113_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2525533.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(27431..27760)
                     /gene="mobC"
                     /locus_tag="PW113_001208"
     CDS             complement(27431..27760)
                     /gene="mobC"
                     /locus_tag="PW113_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2525534.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(28018..28388)
                     /locus_tag="PW113_001209"
                     /pseudo
     CDS             complement(28018..28388)
                     /locus_tag="PW113_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            28533..28718
                     /locus_tag="PW113_001210"
     CDS             28533..28718
                     /locus_tag="PW113_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2525535.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            28876..29043
                     /locus_tag="PW113_001211"
     CDS             28876..29043
                     /locus_tag="PW113_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525536.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            29661..30320
                     /gene="vanR"
                     /locus_tag="PW113_001212"
     CDS             29661..30320
                     /gene="vanR"
                     /locus_tag="PW113_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2525537.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            30323..31684
                     /gene="vanS"
                     /locus_tag="PW113_001213"
     CDS             30323..31684
                     /gene="vanS"
                     /locus_tag="PW113_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2525538.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(31775..33076)
                     /locus_tag="PW113_001214"
     CDS             complement(31775..33076)
                     /locus_tag="PW113_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2525539.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            33355..34161
                     /locus_tag="PW113_001215"
     CDS             33355..34161
                     /locus_tag="PW113_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDL2525540.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            34179..34886
                     /locus_tag="PW113_001216"
     CDS             34179..34886
                     /locus_tag="PW113_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL2525541.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            34883..35854
                     /locus_tag="PW113_001217"
     CDS             34883..35854
                     /locus_tag="PW113_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2525542.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            35847..36875
                     /gene="vanB"
                     /locus_tag="PW113_001218"
     CDS             35847..36875
                     /gene="vanB"
                     /locus_tag="PW113_001218"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2525543.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            36881..37489
                     /locus_tag="PW113_001219"
     CDS             36881..37489
                     /locus_tag="PW113_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2525544.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(37555..37728)
                     /locus_tag="PW113_001220"
     CDS             complement(37555..37728)
                     /locus_tag="PW113_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525545.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        10371 a         8929 c         9659 g         8863 t
ORIGIN      
        1 ctaatacgga cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa
       61 ataccggatt agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg
      121 gccgatcagt agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga
      181 atgttgatgt aagagagaat ttacaaagct cttcaaatgg tcaaggtact aagacaattg
      241 atgttcgaga aaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa
      301 gagagaatgt acaaaattct acaagtagtc aagggtttaa atcaatggat gtaagagaga
      361 atgtacaaag ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc
      421 aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa
      481 agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg
      541 aaaatgataa gggatcaaaa ccaagacgaa gatttaccta tgaagacaac gagttattca
      601 gagatacact taatgatcct aatccgcttt tcgataatct attaaaaaaa taaaaggggg
      661 aattgtcatg aaattaattc taaagattag tacgcatgat ttcttagaac ttgacgaatt
      721 atgtgaatca gtaaagaacg caacaaatga aaaaatcacg gtcactaatt atattgaact
      781 gatgattcaa gaagaattaa agatgaaaca tgagataaaa aacaacttga aatattgtta
      841 tctagaaaaa agtgaagaga taatccatga attaaatggc ttttaaaaat tgtatttgac
      901 atgaatttac gacaaggaat tagtggggat taagcgatta ttctcttcca ctttagagaa
      961 ataatgggtt aaaaagccag aatacgtaga gcaatattga caacctataa aggagctatt
     1021 gttgaaaatt gaagtaacaa aaagataaag ggaggtttac atctatcaga ttaaaagtga
     1081 tagtacaccc atgaaacctg caaaagagtt gaacctaatg tatatcggtt taactctttt
     1141 tatttgcaaa ttaaaaaaat atcaccgaag tgacgaatga aactatgacc aaattaagca
     1201 agaaaatata tcttgcaaat aaagtagtat aaaaagatat tgataaagcc aatcaaaact
     1261 ttgaatttca aacgttagat aatcaaacag atacaaaaaa tgaaatcagt tctacgcgta
     1321 cgattattaa aaataataaa ttttcactag aagaaaatgg aaataaaatc aaataattag
     1381 gaagaaaaaa caaagcaatt agaaaagcct gcaagcaaat ttgagtaatc gagatccaat
     1441 ttttttaagg aagtttaaca aaaatatgta attctattat cactattaaa gagaaagttg
     1501 gagcggaata taaagaaaag agaaaagtaa ttaaacgcaa aaaaatgtag aacttaaaaa
     1561 atataaagag gaacaaccgt taaaattagg aatgatgttg tgtaaacatt ataattgcta
     1621 tgatataaat gaagtgttaa agaaagcaaa tttaaaccaa ttttgtgaac agaaaagcct
     1681 tagtcactat gaccaaaact acggacgaaa ctatgaccaa aagtaggtac taaattatga
     1741 ccgaaaatag gtccaaaatg gattttttta tgatatcaaa aattatgaaa tgattttgaa
     1801 agaaaaccag ttggcacagc caacttagcc accttcctgt aggaaggtgc tatcagtttt
     1861 cccttatgct cttttctttc ttttttgttt catatgtcaa atgccatttt gaaatgtacc
     1921 atcttaagaa atttatcctt aaaaacaatt gcgttattgg ataaaaagat gctacattat
     1981 cttaaaaaag gaatcaaata tgatgaattt agtggctagc atatcgtgtg attaagatac
     2041 tttatagtgt aagttgaagg agaaaaaata tttaaaatat acttcccatt tcagtgggaa
     2101 gtaaaattaa tcttgttgtt tctactagta atgtatcttt ttggaaacta tgtaacgatg
     2161 taaacaagat ttttttcaga agaaaaaacg ataaaagttg tgataaacga tttaaaaaat
     2221 aggagaaaat atatcaagcg catggtatga aacgcacatt taaaaagatt cgactaacta
     2281 aaaaaataag tttacaaagc attgttgtgt attacaagtg atctatacac tgaaaaatga
     2341 tagagaaaat ctgatacgtc cgcaacaatg gacaattgaa aatgaaagtg aggaaattgg
     2401 aagaatggct gatgaaaata aagttgttcc gcttgaaaca ctagtgataa ataaggaaga
     2461 gtttgagcaa ctttttcttg atttttttgt tcaatatgac aggatttatg attttagttc
     2521 gtcactagat ttatttgaat tagaggcaga aataatagaa aacagtcgag aggagaatgg
     2581 taaaatcatc atttcagtag aaaaattcaa tgaattattc tcagaaacat ttgaaataga
     2641 tacagattat tatcaaccac aagaagtttt ccaatcattt agaaatcaga ttattcgctc
     2701 aggtaagacc gtaaactgaa aaacaataaa agaaacgttg aaagaataat tattccttca
     2761 acgtttcttt tttagaatgt tttaatgacc aaagcagttt cattgtcagg cttaggtaat
     2821 tcatgaaatc ccgtatttat agtgctcaat tgatcaacta accatgtttt actttttgaa
     2881 ttgtagataa atctaaaatt aatatcgtta ttatcgtgtt gaaggggcgt ctccaaataa
     2941 tctgctgtat attcatagtc aacgtaacct gctaatacaa aagagagtat ccctttattt
     3001 aatgttagat ttaatggata atcatacgac tttatttttt tcaacttcat attgtacata
     3061 tcttcagcca taaaagttga gttcgtgatt tcatcttggg tctcattaaa aaagttatcc
     3121 gaataatcgc ttatttttga aaagtcttta ttggcaccag cctcaaaaaa ttgttcaact
     3181 ttttcagtat aaagttgtgc taacgctcct ttttcaatat taactttaat attttcatgt
     3241 ctatcaattt gtgtgctgtt cagtaaggga agatcagctt ttagttttat atcgtcttga
     3301 tttctttcaa tttcaattgg caaaatcttt tcagtagatt cagtttctga taaaggaaca
     3361 tctagttttt tatcatgata ataagcttca aatccatctg gaaccggaaa aaaatgtatt
     3421 gggtaattct tttctgaggt tgttgtacta gtttccgtac ttgtagaact tgtgtcagaa
     3481 gtggtacacc cgcttatagt tccgatgagt aaagcgcaag ttagaatgat tgcaaatttc
     3541 ttcaaaaaaa ttcctcctta aaattgaata tggactaata ctagtcccat tgtagcatac
     3601 cgaatcttta agattaataa gaaaaaggac taattttagt ccaaaaggtg tgctattctt
     3661 gaaagaggaa aaaattattg ataaagaaaa tgtaatcggt agcaatatta gacgagtccg
     3721 attagaaaaa ggaattggtc aaaccgagct gattagagac ctacagcttc gtaaaattgc
     3781 tataactagg gaaacgttgg ttaagattga aggtggccgc caacacatta agttggatca
     3841 attgaaagcc atcaaagata ttctacaggt gtcttatgaa gatttgcttc aatgactact
     3901 tgtagtcatt tcgtttgaaa ttattatgtt acacccttat gtgtcacgaa gaaatactgg
     3961 tataataatg aatgggatat gtaacgatag ggtatactgt tatgttgcct aataaattgt
     4021 ggatcaaaat acaagaatgg agggatatcc ttgtataagg catatgaatt ggattttaca
     4081 aatgctgact taaatgcatt ctcctcaagc tcgcaatcat atacacatgt agtaaatcaa
     4141 atagatcaca accaaaaatc tattaacaag agaatagaaa atctatttga aagtgaaaat
     4201 gatttagttg attattattc taaggacagc aaaattctag atgcagacga aattattaaa
     4261 gattggtttc caactataaa agcagatatt tttatatctc actctcatgc tgatgaaaaa
     4321 ttagctatac gttttgcatc atggctattt gaaaattttg gtcttactgc atttattgat
     4381 tcttcggttt ggggatacag ttctgattta ttaaaaaaaa tagaccaaaa atattgctat
     4441 aaggaacaaa caaaaacata tgattatgat aaaagaaatg taacaactag tcatgttcat
     4501 atgatgcttt ctacagcttt aaataatatg attgatagta cagaatgtct gttttttctt
     4561 aatacaccta attcaattag tttgagcaat gaaatcacta atgaacagaa atttacgtat
     4621 tcgccatggc tatatagcga actgacgaca gcttcaatag tggaaaaaaa gaatccacga
     4681 ttggaaagta atcctcaaat gagcaccgag gatgttagaa gcattataaa gcactattct
     4741 tcgcaagaaa atctgcaaat aaaatattct ttaaatttaa gtgaattaac ttatttacga
     4801 aaagctgatt tgaaaaaatg ggctttagat gctgtggaat gcactaaagg aagtaataca
     4861 ttagatattt tatataaaaa caccattttc actaatctct aatcaattta ctgaatcgaa
     4921 aggaaccggg tagaatggat aagaaagttt taataaagag gttctctgaa gctattgaaa
     4981 aaggaaatgc agcaatattt gcaggtgcag gattatcgat gtcacaagga tatgtaagtt
     5041 ggccggaact acttaatgat cctgcaacag aaatcgggtt ggattctaaa aaagaaacag
     5101 acttagttac tctggctcaa tattataaaa atgaaaatgg tggatcaaga ggaattttga
     5161 atcagatact tatggataat tttggtgaag aattagaaat atctgaaaat catcgaatac
     5221 tagcaagttt acctattgaa acttattgga caactaatta tgatcatcta atagaaaaat
     5281 caatccgaga agcttataaa aaccctcagg ttaagaaaaa ttatactcag ttagcaacaa
     5341 ctaatccaaa tgtggataca attgtttata aaatgcatgg tgatatagat gatgtcagtt
     5401 cgacagtaat aacaagagat gattatgaaa aatatgatga ttcgtacgcg ttatttaaag
     5461 aaactttaaa aggagatttg ttaacaaaaa cttttctctt tttaggattt agttttacag
     5521 accctaattt agaaagaatt ttatcagata taagatgggt tttgagagaa aaccaaagac
     5581 ctcattattg tataatgaga aaaatattat agaaaaaatt ttaggttatc gctgaaagcc
     5641 ccggaaatac gggcatttcg caggatatga gaactcaatt tactaccaat ttactacttt
     5701 cggattgagc cgcatatatg ctgaaccata ctgctaatac ccgccacccc aaatacggca
     5761 catcataaat tgaatacgac accgtaaacg cggcgttcct ctttcccgtc atgggagaac
     5821 aggacgccgc ttttttatgc ccaaatagag aaaggaggcg acgccatgct atttacttcc
     5881 ggccctgacc gggcgtttga acggctgatg caggaaaagc ccggtttcga tcactacgac
     5941 ggcggcgggg cggacgcgcc ggaggattgc ggcacctgcc gattttaccg cccggattgg
     6001 aaatatcaat tctgccagtt tgcagagtgt ccctatcagc cgggcaagct gaccgactat
     6061 gcagcggtca aattcacagt gaaaggagcg aacaacgcta tggcagtttt ccgtgtggaa
     6121 aagaacaaag gctatacggt gatgtcaaat caccacctgc ggaataagaa cctgacctta
     6181 aaggccaagg gcctgctgtc gcagatgttg tccttgccgg aggattggga ctatacccta
     6241 aaaggactgt cctatatcaa ccgggaaagc atcgacgcga tcaggacagc ggtatgggag
     6301 cttgaaaaag ctggatatat ccggcgtgag cagggccgtg acgcaaaagg caaaatggcc
     6361 gatatggtct ataccatcta cgaacagccg gtattggaaa acccggtatt ggaaaatcca
     6421 acatcggata acccggtgtt ggaaaatccg acatcggata atccggcgtc ggaaaatcca
     6481 acgcaattaa ataaagaagt acaaagaact gacttatcaa aaaaagaaaa aataattaca
     6541 gatggacaaa gtacccattc cattcctatc ccatccccga cccctgcccc tatggagggc
     6601 gaggcagcgg ctacgccgcc ggaacggaaa aggacaggaa aggatgaggc tgtacggatt
     6661 tatcgggaaa tcattttgga gaacatcgag tatgcctatc tcattcagga caggagcatt
     6721 gaccgtgaac agcttgacga aatcgtagac ctgatgctgg aaaccgtctg caccgcccga
     6781 aagacaatcc gtgtcgccgg ggacgactac cctgccgagc tggtgaaatc caagtacatg
     6841 aagctgaccg gcgaacatat ccgctttgtc cttgactgtc tgcgggagaa cacaacgaaa
     6901 gtccgcaata tcaaacaata cctgcgggct atgctgttca acgcccccag caccatttcc
     6961 aactactaca cggcccttgt cgcgcacgac atggcacagc ccgattgggg aaagcctaaa
     7021 tccggcctgc cggactattc ctgcggcgag ggcgaaagcc tgtaacgaga aaaggaggac
     7081 gaccctatga agcagggcgc attgattttt gacgagcgaa cagaccgtta cgacatccgc
     7141 tttgatctgg ccgactatta cggcggcctg cattgcgggg agtgtttcga cgtgatgatc
     7201 ggcggcaaat ggaggcccac ccgcattgaa atggccgaaa actggtatct tgtcggcatc
     7261 cgggccgacg acctttccgg cctgcgggtg cggatttgac ctatatctac ctgcggcgtc
     7321 tggtggttcc gccttagacg ccgcttttga ctttctgaca aggagggatg cgattgcagg
     7381 aagaagttac tcaaaagacc attgccctgt ccatgaaaac gggcaagctc acggctcaag
     7441 tgctgcaagc ggcgctgaaa aagtacctgc aacaccgggc caagggcaaa aacacgctgc
     7501 accacggcca gcagagccta aagcagctta aaaagcatgg ggctgatctc tccaacattg
     7561 agatcaccga ggccaacatt ggagcgttca agccatgcgc caagaaatac ggcgtggatt
     7621 ttaccttacg aaaagataag accacccagc cgccccacta cgttgtgatt ttcaaggcca
     7681 aggatgcaga taaccttgat ctggcgtttc gggagtttac cgccaagacg ctttccaagg
     7741 agcagcggcc ctctatccgc aaggttttgt ccgctgtgaa gcaaaaggcg gcggcccaga
     7801 ccaagcagcg ggccaaggag aaaatcaagg aaagggggct ggaactgtga agcctgacgt
     7861 aaagaaattg ctgatcctga acctcccgta tctgattttt gtgtacctgt ttgcgaaatg
     7921 cggggaggcg taccggctgg ccgctggtgc tgacctgtcg gggaagctcc tgcattttgc
     7981 ggatggtttt gcggcggctt ttgccaaccc tctgccaagc ctgcacctgt ttgacctgtg
     8041 tatcggtatc gccggtgccg tgatcgtgcg gctggtggtg tactgcaaga gcaagaacgt
     8101 gaagaaatac cgcaaaggca tggagtatgg cagcgcccgc tgggggacgg ccaaagacat
     8161 agcgccttac atcgacccca agtttgaaaa taatatcctg ctcacccaaa cggaacggct
     8221 taccatgacc ggcaggccca aagaccccaa gacagcgcgg aataaaaacg tgctggtgat
     8281 cggtggcagc ggttcaggga aaacgagatt tttcgtgaaa cccaacctga tgcaatgctt
     8341 tccgaccacg gactatccga cttcgtttgt ggtgacagat ccgaaaggca ctttggttct
     8401 ggaaaccggc aggatgttcc agcaggcggg ctaccgcatt aaaatcctga ataccattaa
     8461 cttttccaag tccatgaagt acaacccctt tgtctacatc cattcggaaa aggacgtgct
     8521 aaagctggta aataccctga tttccaacac caagggcgag ggggaaaagt cagcggagga
     8581 tttttgggtg aagtcggaac ggttgtttta ttcggccctg atcggctata tctggtacga
     8641 ggccccagcg gaggaaatga acttctcaac gctgctggaa atgataaacg ccagtgaagc
     8701 ccgcgaggac gacccggagt ttcaaagccc cgtcgatctc atgtttgagc gtttggagca
     8761 gaaagacccc gaccattttg cggtgcgcca gtacaagaaa ttcctgctgt ccgcaggcaa
     8821 gacccgttct tccatcctga tttcatgcgg tgccagactt gcgccctttg acatccggga
     8881 ggtacgcgag ctgatggagg acgacgaaat ggagctggac accatcgggg acgaaaagac
     8941 ggtgctgtat ctcattatga gcgatacgga cacgaccttt aatttcattc tggctatgct
     9001 ccaaagccag ctaatcaacc tgctttgtga ccgggctgat gatgtgtacg gcgggcggct
     9061 gcctgtccat gtacgcctga ttttggatga atttgccaac attggccaaa tacccaattt
     9121 cgataaactc attgccacca ttcgaagccg tgaaatatcg gcctctatta ttttgcagag
     9181 ccagtcgcag ctaaaagcga tttacaagga cgccgccgag atcatttcgg ataactgcga
     9241 tagtgtgctt ttccttagtg ggcgcggcaa aaatgccaag gagatttccg atgcgctggg
     9301 gaaagaaacg attgacagtt tcaacaccag cgagaaccgg ggaagccaaa cgtcccacgg
     9361 attgaactat caaaaattag gaaaggagtt gatgtcccaa gacgaaattg ccgtgatgga
     9421 cggcggcaag tgtatcttgc agctacgggg tgtgcgcccg tttttatcgg agaaattcga
     9481 tattaccaag catccccggt acaaatacct tgccgacgcc gacaagaaga atacctttga
     9541 cgtggacaga tttttgacta tcacgcgccg gaaacggcag caagtggtaa cgcaggatga
     9601 agtattcgac ctgtatgaaa tcgacctgtc ggacgaggac gccgccgctg aataaacagc
     9661 ggcttttttc attccaacaa caaaatcaaa aaatcattat gagccggaaa ccggcagaaa
     9721 gaggtaaatt tatggcattt tttaattccg cagttactac tttgcagact atcgtgatcg
     9781 gactgggcgg cgctctgtgc gtatggggcg gcatcaatct gttggaggga tacgggcagg
     9841 acaatccggg ggcaaaatcg cagggtgtca agcagcttgt cgctggcggc ggcgtggccc
     9901 tgatcggtgt cactctggtt cccctgctgt ccgggctgct gggctgatcc tggcctttgt
     9961 ccctgtccaa acccttaact aatcgcctcc gcgtcctccc ggccagaccg ggagggcgcg
    10021 ggaaaggagg tgctacccgt gattagtgaa atcattgaag aatggataaa aggcatcctc
    10081 atggacggta tcacgggcaa cctgtccggg ctgttcgata cggtaaatgc caaagtcgga
    10141 gaaatcgccg ccgacgtggg acgaaccccg caggcatgga acagcggtat tttcaatatg
    10201 ctgcacagcc tgtcggaaac cgtgatcgtc cccatagccg gggccatact cgcccttgtc
    10261 atgtgctatg agcttatcca gatgataacg gaaaagaaca atatgcacga ctttgacagc
    10321 tccatgttct tccgctggat attcaaatcc gcgtttgcaa tcctgatcgt aacgaacaca
    10381 tggaacatcg tcatgggcgt attcgacgcc acccaaagcg tcgtcaatca aagcgccggt
    10441 gtgattatcg ggcagacatc cattgatttt aacaccctga taccagacct tgaaagccaa
    10501 ttggaggcta tgagcgtggg cgctcttttg gggctgtggt tccaaaccct tgttgtgggc
    10561 ctcaccatga atatcctttc catctgcatt ttccttgtga cctatggccg catggtggaa
    10621 atctatgttg tcacgtcgct ggggcctatc ccccttgcca caatgggcaa cgcggaatgg
    10681 cgcggcatgg gccaagggta tctgaaatcc ctgctggcgc tgggcttcca agccttttta
    10741 atcatggttg tggtgggtat ctacgcggtg ctgatacaga atatcggttc cgcttccgac
    10801 atatccggcg caatctgggg ctgtatgggc tatacggtgc tgctgtgctt ctgcctgttc
    10861 aagaccggca gtatatcaaa ggccgtattc ggggcgcatt gatcccaaaa tccaagcata
    10921 cacaggagga acttgtttat ttcaacgtac aacatcattt acgctgaccc gccgtggcgg
    10981 tatgactgca aaaacgtaca gggcgcagcg gaaaatcact attccaccat gagcattgac
    11041 gagctgtgcg ccctgccggt ggaacggctg gcgtccaaag actgcctgct gtttttgtgg
    11101 gccacattcc ccatgctgcc ggaagctctg cggttaatca aagcgtgggg cttttcattc
    11161 aagaccgtgg cttttgtatg gctgaaacag aaccgtaaaa gccttacatg gttttacggg
    11221 ctgggccgct ggacaagggg aaacgcggaa atctgcctgc tggcaacaca cggcaagccg
    11281 aagcgtcgtt ctgcctctgt ccatcagttt attatcagcc cgattgaaca acacagcaaa
    11341 aagccggatg tgacccgtga aaaaatcgta gagctggcgg gcgatttgcc ccgcgtggag
    11401 ctgtttgcca gacagaaaac ccccggctgg gacgtgtggg ggaacgaggt ggaatgtgac
    11461 cttatcctga cgtgaaagga ggtgccaaat ggcttatgta accgtcccca aggacttaac
    11521 ccatgtgaaa agcaaggttg tgtttgggct taccaaacgg caactgatct gtttttccgg
    11581 ggcgctggtg atcggcgtcc ccttatattt tctcacccgt gactatctta ccaacagcgc
    11641 ggcggccctg ctcatggtat ttgccatgct gccgggcttt ttgcttgcca tgtacgagcg
    11701 tcacggccag ccccttgaag tggtgattat gcagatcatc cagtgctgct tcatccagcc
    11761 aaaggagcgt ccctatcaga ccgacaacac ctacaccgcc cttgtgcggc aataccagat
    11821 ggaacaggag gtaaaggcca ttgtccaaaa aggaaatccc aagagaaacc gaaaaaacaa
    11881 agctgacccg cgcccagcga aaagaaattg acgcggtaat ccgtaaatat aagggcgacg
    11941 gcaggcccca cacggcgcag cagagcatcc cctacgaggt catgtacccg gacggcgtgt
    12001 gccgggtatc gcccggcgtc ttttccaagt gcattgagtt tgcggacatc agctatcagc
    12061 ttgcccagcc ggacacgcag acggctatct ttgaaaaact gtgcgacctg tataactatg
    12121 tggacgcctc cattcatatc cagttttctt tcctcaatcg gaaagttgac ccggtgcagt
    12181 acgccaagag ctttgagatc gcgccgcagg gggacgactt cgacgacatc cgggccgagt
    12241 acaccggcat tctgcaaaag cagcttgcaa acggcaacaa cggcatggta aagaccaaat
    12301 acctgacatt caccatcgag gccgaaagcg tgaaagcggc gcgagccaga ttgaagcgca
    12361 tcggctttga cctgttaggc tatttcaaga gcatgggggc cgtagcccat gtgatggacg
    12421 gctgggaacg gctgaacctg ctgcatggcg tgtaccaccc ggacggcgag atattcaatt
    12481 ttgactggaa gtggctggcc ccgtccggcc tctccaccaa ggattttatt gccccgtcct
    12541 ccctgtgctt cggcaatgca aagacattcg gcatgggcgg caaatacggc gcggtaagtt
    12601 tcctgcaaat cctgtccccg gagctgtcgg acgatatgct ggccgacttc ctcaatacgg
    12661 aaagcggtgt tttagtcaat ctccatgtgc aggccattga acagaccaag gcgatcaaga
    12721 ccatcaagcg caaaattacc gaccttgacg ctatgaagat tgcggagcag aaaaaggccg
    12781 tccgcagcgg ttacgatatg gacattcttc ccagcgacct tgccacctac ggcgaggacg
    12841 ccaagaagct gctgacaaag ttgcagaccc gcaatgaacg gctgttccag ttgacgttcc
    12901 ttgtcctcaa cgtggccgac accaagcaaa agctgaataa cgacgtgttc caagccgcag
    12961 gcgtggccca aaagcataac tgtccccttg tccggctgga ctaccagcag gaacagggcc
    13021 ttgcaagcag cctgccgctg ggcgtcaatc agatcaagat acagcgcagc cttacgactt
    13081 ccagcgtggc cgtgttcgtc ccctttgtga cgcaggagct ttttcagggc ggcgcggcca
    13141 tgtattacgg catcaacgcc aagtcccgca acatgattat gctggacagg aagcaggccc
    13201 gttgccccaa tgccttgaag ctcggcaccc ccggcagcgg caaatccatg agctgtaaat
    13261 ccgagatcgt cagtgtgttc ttaacgacgc ctgacgatat ttttatttca gacccggagg
    13321 ccgaatatta cccccttgtc aaacggctcc acggacaggt aatccggctg tcgcctacca
    13381 gcaaggactt tgtgaacccg ctggacatca acctgaatta ttcggaggac gataacccgc
    13441 tggccctgaa atccgacttt gtgctgtcgt tctgtgagct tgtcatgggc ggcaagaacg
    13501 ggctggaagc cattgaaaag accgtgatag accgggctgt gcgcgtgatc taccggccct
    13561 atctggcaga cccccgcccg gagaatatgc cgatcctgtc cgacctccac aaagcgttgt
    13621 tagatcagca tgtcccggag gctgaccggg tggcgcaggc ccttgacctg tatgtgtccg
    13681 gctccctgaa cgtatttaac caccgtacca acgtggacat cggcaaccgg cttgtgtcct
    13741 ttgacattaa ggagctgggc aagcagctca aaaagctggg gatgctcatt gtccaagacc
    13801 agatatgggg gcgcgtcacg gccaaccgaa gccaaggaaa ggccacatgg tattttgccg
    13861 acgagttcca tttgcttttg aaagaggaac agacggcggc ctattctgcg gagatttgga
    13921 agcggtttag gaaatggggc ggcattccaa caggtgccac ccagaacgtc aaagacctgc
    13981 tttcctcccc ggaaattgaa aatatccttg aaaacagcga cttcattacg ctgctcaatc
    14041 aggcgtccgg ggatagaaaa atccttgcgg aacgcttgaa tttgtccacc gaacagcaga
    14101 agtacatcga caattccgaa cccggcgagg gccttttgat ctttgaaaac gtggtgctgc
    14161 cctttaccaa ccccatcccg cacaacaccc agctttacaa gattatgacg acccgcttaa
    14221 acgaggtggc gggagtatga cccggcgacg ccacaaagga ggtgaaccac catgaaagag
    14281 ctgaaagcca aggcgaaagt gacgcaggcc atgacccgcg acgggctgac gctggaaaat
    14341 caggccaccg gcgaggtgga gaacatttcc agccgggagg ccgaacagga tttgtccgcg
    14401 ccggggccgg gcggcaccgc cgataagctg ctggaacggg ccgacgccat ccatgacggc
    14461 cataaggcca agaaagccgc gaagcaggcc ggggctgtgg ccgccgaggg ggacgctgcc
    14521 ctgcaccggc catccgcccg gctccaactg tcggaggaag aacgggccga cccggattta
    14581 cagccctata tccgaaaatc ggacaaacgg gccgataagc tggataaggc ccgcgccgcc
    14641 atccccaaga agcgcgtccc ggtcaaggaa accgtctttg acgaggcaag cggcaaagca
    14701 aagaccgtcc tgcactttga caaacaggac aaggggcctc cctccctgaa acccaatccg
    14761 gcaggccgtc ctgtttccga ggtttttcta ttcggccacg ggaaaatcca tgaagtcgag
    14821 catgaaaacg tgggtgtgga gggcggccat aagggggagg aacttgccga gcggcaggcc
    14881 ggaaaagcca tccgcagcac catccggcac cacaggctga aaccctactg ggacgcggaa
    14941 aaggccgagc gcaggctatt gtctgccaac gcggagtatt tctaccagaa atctttgcgg
    15001 gataacccgg agatcgcaca ggcggccagc aaccccattt cccggttctg gcagaaacag
    15061 cagatcaagc gccagtacgc caaggccgcc cggacagcgg gccagaccgc aggcgcggcg
    15121 gcacagggcg cggccaagac agccggggcc tccacgaaag cggccaaggc cgcagccaaa
    15181 gaaagtgaac gggcggcctc ctttgtggcc cgccactgga aaggggcgct ggccgtcctt
    15241 gtgttggggc ttttgatgct gcttatcatc ggcggcttac agtcctgcac cgccatgttc
    15301 ggcagcgccg gaagcggcat cacggcgtcc tcttactttt ccgaggatag cgatatgttg
    15361 gccgccgagg acgcctacgc ggagctggaa gccgagctgc aagactatct ggacaactac
    15421 gaggccaccc atgactatga cgaataccat tttgaccttg acgagatcag ccatgacccc
    15481 tatgtgctga tttccattct gtcggcgctc catgacggga tatttaccat cggggaggta
    15541 cagggtgatt tgtccatgct ctttgacaag caatatatcc tgacagaaaa cgtcgtggta
    15601 gaaacccgtt accgcaccga aaccgataca tggacggacg cggagggcaa cacccataca
    15661 gacacctacc aagtgccgta tgactattac atctgctacg tcacgctgga aaacttcgac
    15721 ctgtcccatg tgccagtcta tatcatggac gaggaaacgc taagtttcta ttccctgtat
    15781 atgtccacgc taggaaaccg gcctgatctg ttttccggca gccagtaccc caacgcctcc
    15841 accctgacgc cgccgaccta ctacgacgta cccccggagg ctttggagga tgccaccttt
    15901 gccgccatgc tcaaagaggc ggaaaaatat atcggttatc cctatgtgtg gggcggcagc
    15961 tctcccagca ccagctttga ttgcagcggc ttcatttcgt gggtaatcaa tcatagtgga
    16021 tggaacgtgg gacggctggg ggcgcagggc ctatataaca tctgcacccc tgtttccagt
    16081 tcgcaggcca agcccggcga ccttgtattt ttcaagggaa cctacgacac ccccggcgtg
    16141 tcccacgtcg gcctgtatgt gggcaacaat gtgatgctgc attgcgggaa cccgatttcc
    16201 tacacgaacc ttacttcaaa ctactggcaa tcccattttt acagttacgg gcgtttacct
    16261 taacgccaga aaggagcttt caattatggc aaccaaactt gaccgcatcg aaaaggacat
    16321 ccagaaaacc aagtctaaga tcgcggagtt tcagaaacag cttcgggagc tggaaacgca
    16381 gaaaaccgaa caggagaatt tgcagatcat ccagcttgtg cgcggcatga acatgaagcc
    16441 ggaggaattt gccgcgttct tacgcagcgg cgcaatgcag gccgcccctg ccgcaacccc
    16501 ttaccatgaa caggaggaca atgcccatga agaataagac cagacggatt tcccgtattc
    16561 tccccgcact tctggccgtg atgctctgcg tcacggcctt tcccgttacc gcccttgcag
    16621 gcggcaatga cccggcaccg gccccgctgc cggaggctac aaccgaaccc gctacgggcg
    16681 gtttggagcc tgaaacagac gaaaccggca ccgccacgga cgggacagaa accgaggccg
    16741 aaaaagatac cggccctctg accggcaagg acatcaccga cctgttttcc gcgctgttcg
    16801 gttccaaggt gagcattgcc gccacggacg acggcatcca gatcaccacc ggcaccgaca
    16861 aaaaagaacc tgaacagacc ggcaccgtca ccaccaacgg cggcaggctg aatgtccgca
    16921 ccggcgcggg gcttgacaag accgcgttca cccagcttcc caatgggacg acggttgagg
    16981 tagtcggcac agacggcgac tggattaaaa ttctcttgcc ggagcgcatc ggctatgtcc
    17041 attccgacta tatgaccgtc agcgaaaagg aagtagcggc taccggcgag ggcggttttt
    17101 ccctctccat tgacccggag gaaattgctt ctctgctgga actgttcaat ggcggcgggg
    17161 ccagcgccgc cctgtccccg gacgggaacc tgtcactgat tgacgacatc ggcagcgccg
    17221 cgaaaaatgg caagcagttt atcaccgtgg aaaccaaaaa cggcaatgtg ttttatctga
    17281 ttattgaccg ggacgacgag ggaaaagaaa ctgtccattt cttaaatcag gtggatgaag
    17341 ccgatctgct gacgctgatg gaggacgggg aaaccgtcac ccccgctgct gtctgctcct
    17401 gtaccacgaa atgcaaggcc ggggctgtga acacgaattg cccggtatgt aaaaccaacc
    17461 tgaccgagtg cagcggcccg gagccgcagg aaccccagcc ggaggaaccc gaagccccgg
    17521 aggaagaacc caagggcggc gcgggcggcc tgatcgtgtt cctgctggtg gcgctggcgg
    17581 gcggcggtgc ggccctttac tatttcaagt tcaaaaagcc caaggctgaa accaccggca
    17641 gcgacgattt gggcgagtat gattttgggg aggacgagga tttggaggac gaggaacccg
    17701 aaaccgagtt tgacccggaa gctgattttg tgagtgaaca ggaggacgag gaatgacata
    17761 taaacttgta atcgcggaaa aacccagcgt ggcccgcagc attgcgggcg tgatcggggc
    17821 cacggagaaa cacgacggct atttggaggg gaacggctat ctggtgagct ggtgcatcgg
    17881 acatctggtg tcctttgccg acgccggacg ctatgacgag cgtttcaaaa aatggcgcta
    17941 tgaggatttg cccattctcc cggaaacatg gcagtacatc gtccccgacg acaagaaaca
    18001 gcagtttgac gttctctgtt cgctgatgca gcgccccgac gtgaccggcc ttgtgtgcgc
    18061 caccgacgcc gggcgcgagg gggaattgat tttccgtttc gtctaccaga tggcgggatg
    18121 ccaaaagccc tttgagcgcc tttggatttc ctctatggag gacagtgcta ttaaggacgg
    18181 ctttgcccac ctgaaaccgg gcggcgacta tgatccgctg tatcaatcgg ccctgtgccg
    18241 ggccaaggcc gactggctga tcggcattaa cgccacccgg cttttttccg tcctgtacca
    18301 caagaacctg actgtgggcc gcgtccagac gccgacctta aaaatgctgg tggacaggga
    18361 cgccaagatt accgatttca agaaagagaa atatcacatc gtccatatca cggcgggcgg
    18421 cgcggacgcg gtaagcagcc gcttttccga cgctgccgag gcaaacaccg tcaaggcggc
    18481 ttgtgcaaaa gcgcaggccg tttgcgtttc cgtgacgcgg gagaaaaaga cggaacagcc
    18541 gcccaagctc tatgacctta ccaccttgca gcgggaggct aaccggcttt tcggctatac
    18601 cgccaagcag acccttgact atgcccaggc actgtatcta aagaagctgc tgacctatcc
    18661 gagaacggat agtcaatatc ttaccgacga tatgctgccc gccgtggaaa gccttgtgtc
    18721 cggcctgtgg gagctggtgc cgtttgccaa gggcctgaac ctttccccgc agtttgaccg
    18781 catcctgaac agcaaaaagg tgtccgacca ccacgccatt atccccaccg ctgaatttgt
    18841 gaagcagggc tttggcactc tggccgagag tgaaaaaaag ctgctgtccc tgatctgctg
    18901 caagctctta tgtgccgtgg ccgggccgca tatctatgag gccgtcaccg ccacatttac
    18961 ctgtgccggg caggagttta ccgccaaggg caagaccgtc ctttcccccg gctggaaaga
    19021 gattgaacgc cggttccgtt cttccttaaa gaccgacgcc gacgaggacg ccgaggcggt
    19081 gcgggagctg ccggagcttt gcgagggcca gacctttgca gacgtggccg cctctgtcac
    19141 cgagcatttc accacgccgc ccaagcccta caccgaagat acgcttttgt ccgctatgga
    19201 acgggccggg gccgaggata tgccggagga tgccgagcgc aagggattag gcacaccggc
    19261 cacccgcgcc gccattctgg aaaagctggt gcagatgggc tttgtccaga gaaaaggcaa
    19321 gcagctcatt cccaccaagg acggcatcaa tctggctgtg gtgctgccgg aggcgcttac
    19381 ttctccccag cttactgccg aatgggaaaa ccggctgacc gagatcgcca aggggcaggc
    19441 cgatcctgac gaatttatgg acggcatcga gacgcaggcg cgggagctgg tgcagaccta
    19501 ttcctgtatc agtgaggaaa agcagaaatt gtttcaggcc gagcgtgttc ctatcgggac
    19561 gtgtccccgc tgtggtgagg ctgtctatga gggcaagaaa aactactact gcggaaaccg
    19621 ggcctgccag ttcgtcatgt ggaagaatga ccgatttttt gaggaacgca agaaagcgtt
    19681 caccccgaag atcgccgctg cgctgctcaa aaccggcaaa gtcaaggtaa agggcctcta
    19741 ttccgtaaag accggcaaaa cctacgacgg gacggtgctt ttggctgata ccgggggcaa
    19801 gtacgtcaac taccgcattg aacacaggag ctaaaggaca gtaacacagc aagcataggg
    19861 agtgtggcta cactcccggt aaaatccccc ggccgcgtcc ctgccgggcc ggggattttt
    19921 gcttgttggg gaagtcccca accccctatt ttttgaaaca gaacagagaa aggagtgacc
    19981 gcgtgaacga agtattttct atccaagtcc ataaccggca gtttttggaa cagggcaaac
    20041 aaggcgtctg gctggatttg cccaccacaa ccgaaacgct gcaagccgcc ctgcgggatg
    20101 ttggcattac tgccgacaat ccgcaggatt tttttatcaa cgggtattcc tgcccggagg
    20161 acagacacct tgccattccc tatgacatgg tgctggccgc cgacgtggac gagctgaatt
    20221 tccttgccgc ccggctgggc cagcttgacg ccgccgagat cgcagagctg aacgccgccc
    20281 tgcaaaatcc ccgtggcggc tttgagaata tcggccagat catcgactac accgaaaatg
    20341 tggattacta tgtgcatctt cccgacgtta ggggggatgg acagttgggc gactactacc
    20401 tgaaccgttc cggcatggtg gatatgccgg aggaatggaa agccgggatt gatacggcgc
    20461 agtttggacg ccacattgcc cagcaggaac aaggcgtttt tacccagtac ggctatctgg
    20521 tgagaagcgg cgacgaatgg cagcgcgtcc atgagggcca gccggtgccg gaggaatacc
    20581 gggtgatgca ctacccccag ccggaagctg accgggagga accgcaggcc cagcagccgg
    20641 aggcatccga aagccccgac aaagcgccgc ccccggttat cccgatcatg ctggacggca
    20701 tgaacagcaa agaacgcatg aaagtgatta ctgaccgtct ggaaaccggc gtacaggagc
    20761 tttttgaaag tagccgctat atggcttact tatccgctat gtccaagatg cacgattaca
    20821 gtctgaacaa tacgctgctg atcgccatgc agggcggttc gattgtggcg ggctataaca
    20881 ggtggcagga tgaatttgac cgccatgtga agcggggcga gaaaggcatt aagatactgg
    20941 ccccggcccc gttcaaggtt aagcagcaga tggagaaaac cgacccggcc acccagcagc
    21001 ccgtgatcgg cgcggacggc aagcccgtga ccgaagaaca ggaagtgacg gttccggcct
    21061 ttaaggtggt gacagtcttt gacatcagcc agaccgaggg caaggatttt cccatgccct
    21121 atatcagtga acttaccggc gacgtgcagc agtatcagga tttctttgcc gcgctggaac
    21181 ggacatcacc ctatcccata agcattgagc cgatttcaga cggggccaag ggccgctgct
    21241 tctacgcaaa acagcgcatt gccatcaacg agggcatgag cgaattgcag accctaaaga
    21301 ccgccatcca tgagattgcc catgccaagc tccacgccat tgaccgggag gcccccgccg
    21361 aggaacaggc ggcccggcca gaccgcagca cccgcgaggt acaggccgaa agcgtggcct
    21421 ataccgtctg ccagtattac gggctggaca cgtcggacta ttcttttggc tatgtggccg
    21481 attggagcag cggcagggag ttatccgagc tgaaagcctc ccttgaaacc atccgggcca
    21541 cggccagcga attgataacc gagatcgacg gccattttgc ccagcttcag caggagcgcg
    21601 aggcccggca ggaacaggag gccgtcacgg ttgacgttac cccggaggca gcggccccgg
    21661 cgcaggccga ggaagaacag gccccggccc ttgatccggc agccgagccg cttgtgactg
    21721 tgctatggag cgaaagcccc cagctaaagg acggccagca aatgccgttg catgaggccg
    21781 acgcgctgtt tgcccgtctg gatgcagcat atccggcaga ggcaggctat gacaaaaccg
    21841 cgttccgcat tgactgtatc tttcagggcg agccggacag ctacgagggg cggcaggatt
    21901 ttggagatag ggacggttcc ctgatcgaac atatccagag ctaccaccaa ttttacgcgc
    21961 aggacgaaaa ctggaaaaag catgtaatcg ctacaaccgg catagagggc tgggagcagg
    22021 acaaggccca tcgggatatg ctgttaggcg agtttgtccc ctatatgaag ctgcattgca
    22081 gccttgccca aatggaacag acggcgcagg gccgcttaca gtccggggaa accctgacgc
    22141 cggaggaaac ggcctatttg ggggcgcttc tctcttatgt gcaggagtgc cgccccatgc
    22201 tcaatcaggg ccagtatcag ttgccggagc cgccgaaact ggctgacttc gaccagagct
    22261 tgcaggacta caaagcacag gtacaggccg agatcgcgca ggaggccgcc gcagccggta
    22321 tgaccgtgga ggaatacgcg gcggctggct atgaagcacc ccaacaggac accttttcca
    22381 tctaccagct tcgaggcggg gaggaaaccc gcgactatcg gtttgagcca tacgagcgtt
    22441 tacaggccgc agggctggcc gtggacagtg ccaactatga ccttgtttat acggcaccgc
    22501 tggataaaag cacgtctttg gaggatattt accgcacatt caacatcgac cacccggcag
    22561 attttagggg acacagcctt tccgtttccg acgtggtggt gctgcaccag aatggacagg
    22621 acaccgccca ctatgtagac agcttcggct atatggaagt gccggagttt ttgcgggaaa
    22681 atcccctgcg taccgccgaa ctgtctacgg agcagaacga aaacatgatt gacggcatta
    22741 tcaacaatac gccgcccgaa ccgcagccgg aggaacccga acagctatct tttgaggatg
    22801 tggcagttgc ttccggccag acacaggagg aacccccgga ggctgcgccc ccgcaaacgg
    22861 ctgtccgcta ttatcccatc aatgagggag ccgcccgccg cgccaaggaa gccatgagct
    22921 tttctgacta ccggcccggc agcgccacgg cggaataccg gcactatgtc gatcaggccg
    22981 tggaaattgc ggagcgccag aaaaagcgtg tggagccaga gtatcacgaa aagatagaca
    23041 gcctccttga cacctacgcc cgcaagctgg ccgagaacat gaataagggc tatgagatta
    23101 ccgcccgcgt cccctctgtc ctgattgccg ggccgtccaa tttcccggta cgggcgaaag
    23161 aaaagcagaa cgccgccagt gaccgcaaca tggaggaatt tcagtacatt cagggcttgc
    23221 ttgacaaaat ccgcagcacc ggcatgggtg gtatcagcgc cgacgatccg caggccgttt
    23281 ccaagctgga aaagaaactg gaaaagctgg aagcgtcgca ggagttgatg aaggccgtca
    23341 atgcctacta ccgcaagcat gggacgctgg acggatgccc ccatctgatc gaaagaggca
    23401 ttgaaaacct gaaagcggat atggcgtcgg gctggcacta tgagaaaaaa ccgttccagt
    23461 cctggcagct ctccaacaat aacgccgaga tacgccggtt aaagggccgc attgaggaat
    23521 tgacccgcca aaaggaggcg gcctatgtgg gatgggagtt tgacggcggc actgtggaga
    23581 tcaaccgggt ggccaaccgc ctgcaaatct tctttgaggg gaagccggat gctgctgtac
    23641 gcgacgagct gaaaagcaac ggtttccgct ggtcgcccaa ggccggggca tggcagcggc
    23701 agttaaacga caccacaatc cgcgtcgctg accgtatcaa gtgcatccag cccctttccg
    23761 gcgaaaagcc cagcgcctta cagcttgcgg cccgcaggga gaaagaaaag ccctctatcc
    23821 gggcgcagct caacgcggcc aagaccgaac aggccaagca gccaccggcg cgggaaaaat
    23881 caaagggatt ggaggtagag taaatgcaga agtttgaaaa tgtggacgtt ctggccgcgc
    23941 tggaacagat catgcggcag aacaccgcgt tttatcaaag tgactttggc attgacaaag
    24001 acattatcag gcgggatgcc gccagcgatc aggccgcaga caagaccctg ctctggatgt
    24061 cccggccctc cgggacgtac tgtttccggg aacgtgatgt tttcctgaaa gacacccgac
    24121 agtacaacac atggaaattt tacggggagc agacccgtga caagatactg gcctacgcgg
    24181 tggagctgac cggcacacag ggcggcacta tccggggcaa tctctatgag ctggactatc
    24241 cgcagcattt ccggcacgtc attgaaaccg cccagccggt gagtgtgaac cggctctttt
    24301 atgagcatgg cacccgtgac atcccggaga gccagcgttt tgacggaagc cctgaccggg
    24361 tattgggaaa ctttttgtac tatgaggccc agccccatga cccggctgtg ctgcaggagg
    24421 cgctacggac ggagcagcac agccgggggc gggccgtccc cggcgatttc aaagtccacg
    24481 tcaccgcttt gcatgacagc cggattgaaa ccgaggcccg gcgtattgtg gacagcctga
    24541 aagatctgtc cgcgcctaac agccccaata aaacgcattt catggtggag atctccccgc
    24601 actttgcggc gattgcttcc accaaggata atgaccggct ctttgccatg ctgccctata
    24661 aatccctgtg ttttaccggg atgaaaggca ggcatggcgt gtatgccgtg attaaccggg
    24721 acgagcgccg ggatgtgagt attcgcaggc cccggccctc tatccgcaag cagctatccg
    24781 acagcaaaca ggccgccagc ccaaaaaagg cggcggccag aaccaaaaag aatgaattgg
    24841 aggtataagg aatgatgcac tttaccgtgg aggaagaaaa cctgatctgt atgtaccaca
    24901 atgcagaccg gcgcagaacg ataaacaggc tcaccgccgt catgccggtt atggatgggg
    24961 atatgcgggc gcaggcccag cagacccttt ccaagctgga acgcatgacg gatgccgact
    25021 atgacggcca gaagttccac ttcacagacg aataacgaac agcagacgcc gggcgcggcg
    25081 gccatgtggt cgcttgcgtc cggcgtcttt tctttttgcc tattcctacg catttagaac
    25141 ccggtattgt tggggatagg atgaagtatt cgcttgcccc tgcaaacatg cgggagaagc
    25201 cttgcagcgg gcggttttca atcccttaaa tgcgtaggtt gcagctataa ttagacccct
    25261 gaaacagata tagccgcgca gcgggtgtat ctgtttcagg ggtgtgcagg tgatagccgc
    25321 aaagcggcgt aagagggtgc agccctcttg ccgtctatct ctgttccagc gggaagctcg
    25381 gcaatgcttc cagcagtttc agggaaatgc gctgccgtat atcttcgtca atgactttct
    25441 tctttttgcc gtttgcctgt ctgatctcca ccgtggaaag ctcgttgatg tagtcagcat
    25501 aatgggccaa cactttttcc gtggcccacc tctcaccggc aacggcggct ttgatcgttt
    25561 catagtccag cagcttttct tgtccgtcca tcctgatacc cccgtcagac tgattttttc
    25621 tatgatacca tattcggaag catggaacca gagaaaacgg cgcttttctg ctggtaaatt
    25681 ctacgcattt agaacagcac ttcggcgggg ataggataaa acacccacat accccgctga
    25741 ccatgccgga aaagccttga aatataaggg cttttacagc tctaagtgcg taggtttcca
    25801 gtatcttttc cgctggcgct cggcctcttt gcggcggcgc atccgtttgg agcaggccgc
    25861 gcagtatttg gcccggttag acttcggcgt gaacgtggcc ccgcactcac agcagcgttt
    25921 cacttcgtcc cggtctttgg tgatctcggc atacagggcc gcatccagcg ggaggacagc
    25981 ggccttaaac cagcggcaca gaagcgaata ggaaatgctc tgtacgcaaa tacattcttc
    26041 gccgtcgtcc agcaacaggc agttgccgtc acaatagttg cagcactcat gcgtcagctt
    26101 ccgggccttg cgatattgag ggtaattcat tcgtggaata ctcatagctc ttgcgtccat
    26161 tcctttcccg gcgtcatacg caaaatgccg tccacattct gcttgatgat gccgtattcc
    26221 tgcatctgct ttttcgcttc gccgtacttc tcatacagcc gttccttttc ctcggccaat
    26281 cgcttgtact cggctttcag ggcgtacaga tcggggagct ttttcacacc ctgtcccttt
    26341 agggcctttg ccgcagcttc atacaaaatc aacgtgcttt catgctcacg ccggaatttt
    26401 gccttatccg ctgcattgcg gtattccata gccacgggcc agagctgctt gtaggtagaa
    26461 atattcttta tcagcaccgc catatccgca agccggtgtt ccatagcttt gagggtagcg
    26521 gcggcctcgt cgctggcggc ggtgatctcg gccactttgc tttccaaatc cgggtaggtg
    26581 tctatcccat gttcagtcag gaaattcaaa gtctttgcgg cctgcttcaa attgtggagc
    26641 ttcgcccacc tctcatagcc tgccgactgc tgggccttaa tgttgttttc cagatcaatc
    26701 cgcaaagaaa tccccttgcg ctctttgggc gctctggtga taacgcggcc tttgatacgc
    26761 tctttgatgg cttcctcggt gtaggcttcc cccaatgtct tgcagcgggt aaagcgttcc
    26821 tgtccgggag cgcggaaaga aataaacttt cccggcttga tctcatagcc ctgcgcctgc
    26881 atgagccgca ggaaatcatc aaaatctttt gcctgcggga tagcggcgtc aatggctgct
    26941 ttgagcttcg ctttccagct cgtccctttc cgctgggcgt cccactcggc atagctcttg
    27001 cccttactct ggccgggctt caccacggac aatccgtttt ccttgcacag cctgtcgctg
    27061 gtgcgccgga tataggcata gctctgtttg ttggaaatgt acttgcgctg atttaccata
    27121 tccacggcac agaaaataat gtgattgtgg acgtggccct tatcaatatg ggtagtcagc
    27181 acatagggat atttcccttg cagcacttcc tcggcaagct gcttcccgat ctcatgggcc
    27241 tgttccggcg tggcttcgcc gggggcaaag gattgtatca ggtggtggcc cagattattg
    27301 cccttttggt atgcctgcgc cagcagcatt tcaaattcaa tgtccgctgt ttcataagag
    27361 caggcaaagg aggccaccag cagcttgccg tcagttttgt ccgggttctc gatgtagtca
    27421 agggcttttt tcagcgttga cttgatagga tgtatctttg taactgccat atctgcgcca
    27481 gcgccccctt tatatcctcg atgtcctgtg cgtacaccgg ccccataccg ttcagcctct
    27541 ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc gcccggatgt
    27601 cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg taggctgaaa
    27661 agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc tccggcgtca
    27721 cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt cctttccatt
    27781 caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg gacggggcag
    27841 gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt tctccactaa
    27901 caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag aaaaaagtgc
    27961 aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg atgccagtta
    28021 cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt caatcgcgcc
    28081 ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga ccctttgccg
    28141 cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag gaagcccgca
    28201 ggctttaata agctgtatga caaccggcag actgggaatg gcccgtcgtt ttcgatgttg
    28261 gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga aacgcgcttt
    28321 gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca atcttcaata
    28381 ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg tggaatgttg
    28441 gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt cttcgagcaa
    28501 aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg aaagaggcag
    28561 gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg ggacgcatcc
    28621 ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa aagccgattg
    28681 acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc ggcgcatttt
    28741 ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac tataagatga
    28801 tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag aaagaaaaga
    28861 tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga aaatgcccga
    28921 aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga aaaggccggc
    28981 atctggattc tttatggtga agtctccaga cattggataa ctgctggtct aatgcgcctt
    29041 tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg aaaatattct
    29101 gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg atgatgaaga
    29161 tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg actgaatctg
    29221 ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc ctgtgtgaaa
    29281 agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga ggcttggggt
    29341 gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca gaatatccgt
    29401 aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca accgctgtaa
    29461 tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt aattcgttta
    29521 aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg acggatgatt
    29581 aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt atgttatctt
    29641 cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat gatgatcata
    29701 tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat gcctgcacag
    29761 atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt attcttgata
    29821 ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg aaaaatgata
    29881 ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg gcgtttgatg
    29941 cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta aagcgggtgg
    30001 aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc aggctgacac
    30061 ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg acacgaaaag
    30121 aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg catgaaatca
    30181 ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc cacactcata
    30241 tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc ggtgtaggat
    30301 accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg ttttttccta
    30361 tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg cacagcaatt
    30421 tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc agccattggt
    30481 ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc tgtttcacta
    30541 caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac tctatgccac
    30601 accaaatgcc aatacatcaa atagttttag acccaacttt ctttatgtgg tacatagaga
    30661 tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt atcaagggct
    30721 gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt tatgcgcgta
    30781 tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg cgaataaaat
    30841 ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg gcgcactggc
    30901 tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc tggaggatga
    30961 aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg cagcttctca
    31021 tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc ttgaaaatat
    31081 cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga tggataggca
    31141 gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga gaatcgtacc
    31201 catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg attttcaaac
    31261 cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc aaattgtcct
    31321 gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg cagttcagaa
    31381 cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa aatgccgcct
    31441 ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc tgttcacccc
    31501 attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa aaagtgggcg
    31561 aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc tccagtatgc
    31621 gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca catcaacatt
    31681 gtaaatattt aaaacttaaa tgattttggg ctctttgtca aataggactg atagagtgag
    31741 ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa
    31801 tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag
    31861 cgactcgttt caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag
    31921 gcaattcgag ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat
    31981 gcagttcttt tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct
    32041 ctttgtcgtg aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt
    32101 cagagatcga aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat
    32161 tgcgccattt ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag
    32221 acaacgcctt taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt
    32281 tcatgattcg tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac
    32341 gatccacaac gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat
    32401 tcatgtccgt cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat
    32461 aagaaacgag agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc
    32521 catccataca agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca
    32581 ttaataaaca acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga
    32641 gtgtccgaga aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt
    32701 gtttttcagt tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc
    32761 tatcgacaat gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt
    32821 gtttcgctaa agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt
    32881 ttttctttcc gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc
    32941 gacgaatttc tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct
    33001 tcgattccac atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat
    33061 gatgaagatc aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg
    33121 gagcttctgt atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc
    33181 agtcctagaa attatagagc cgattttgac cgacaggtat aaccctgccg gtcttttttt
    33241 gctgctacag gaaaactaca gactggctac agggaaagta cagatgcgct tgccataata
    33301 acaatcgtac cagccacaaa tcgtagtttt attgcaaagg aggcattcga tcaaatggaa
    33361 aaaagcaact atcattccaa tgcggatcat cacaaacggc atatgaaaca atccgtggaa
    33421 aagcgggctt ttctatgtgc gttcattatt tcgttcacag tctgcacgct gtttttgggg
    33481 tggagactgg cttccgtatt ggaggcaaca cagataccgc ccatccctgc aactcataca
    33541 ggcagcagca ctgacgtagt ggagaatttg gaggaaaacg ctcttgccac cgccaaagaa
    33601 cagggagatg aacaggaatg gagcctgatt ttagtgaaca ggcagaaccc catccccgca
    33661 cagtacgatg tggagcttga gcaactatca aatggtgagc ggatagatat tcggatttct
    33721 ccctatcttc aagatttgtt tgatgccgca agaactgatg gagtttaccc gattgtcgca
    33781 tccggatacc gaacaacaga aaaacagcaa gaaattatgg atgaaaaaat tgccgaatat
    33841 aaggcgaaag gctacacctc tgcacaggct aaagcggaag cagaaacttg ggtggccgtg
    33901 ccgggaacga gcgagcatca gcttggtctt gctgtggata tcaatgcgga cggaattcat
    33961 tcaacaggca acgaggttta tagatggctg gatgaaaaca gctatcgttt tggttttatt
    34021 cgtcgctacc caccagacaa gacagagata accggtgtga gcaacgagcc gtggcattac
    34081 cgatatgttg gcatcgaagc tgccacagag atgtacaacc aaggggtttg ccttgaggaa
    34141 tatttaaaac cagaaaaatg agaaaaggat gttatgttat ggacagaaaa agattgacac
    34201 agcgtttccc gttcctgctt ccaatgagac gggcgcagag aaaaatgtgc ttttatgcgg
    34261 gaatgagatt tgacggctgt cgctatgcgc agacgatagg ggaaaaatcg ctttcccatt
    34321 tgctctttga aacggattgc gcattataca accacaatac cggatttgac atgatatacc
    34381 aggaaaacaa ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc ttattgataa
    34441 gaccggggga aaccttttct ttctggttgt tcctgcatac gccattgaca attatccagc
    34501 gcagaggtca cgaagtaaag gagtttccag agccaaacag tgacgagatt aaaggggtgg
    34561 atgcaaccat ttcagagggc tggattgatt taaaagtgcg aaacgatacc gactgcacct
    34621 accaaatatg ggtgacctta gatgatgaga aaatcatcgg tcaggtgttc gccgacaaag
    34681 agccccaagc attatacaaa attacaaatg gcagtatcca gtatgtccgt gaaagtggcg
    34741 ggatttatga atatgcccag gttaaacgga tgcaagttgc cttaggtacc ggggaaataa
    34801 tagattgcaa gctgctttat acaaacaaat gcaaaatctg ttatcccctc ccggaaagtg
    34861 tggatattca ggaggagaac caatgagaaa aagtgtgggc attactgttt ttggatgcga
    34921 acaggatgag gcaaatgctt tccgcgcttt atcgccggat tttcatatta tccctacgct
    34981 gattagcgat gcgatatcgg cagacaacgc aaaattggcc gctggcaatc aatgcgttag
    35041 cgtaagccat aagtccgagg tttccgaggc gacaattctt gcgctgagaa aggtcggggt
    35101 aaaatacatt tctacccgca gcatcggctg cgatcacatt gatacgactg ccgccgagag
    35161 aatgggaatc tcggttggca cggttgcgta ttcgccggac agcgttgcgg attatgcttt
    35221 gatgctgatg ctgatggcca tacggggtgc aaaacccacc atgcacgccg tggcgcaaca
    35281 agatttcaga ttggatcgta tccgggggaa agaactgggg gatatgactg tgggagttat
    35341 tggaaccggc catatcgggc aagcggtcgt caaaaggctg cggggatttg gatgccatgt
    35401 gctggcctat gataacagcc gaaaaatgga tgcagattat gtccagcttg atgagcttct
    35461 aaaaaacagc gatattgtta cgctccatgt gccgctttgt gcggataccc gccatctgat
    35521 cggtcagaag caaattggag agatgaagca aggcgcattt ttaatcaaca ccgggcgcgg
    35581 agcgcttgtc gataccgggg cgctggtgga ggcgttggaa agcggaaagc tgggtggtgc
    35641 ggcactggat gtgttggagg gcgaggatca gtttgtttat accgattgct cgcagaaagt
    35701 gcttgaccat ccctttttgt cgcagctttt aaggatgccg aatgtgatca tcacacccca
    35761 tacggcgtac tacactgagc gtgtgctgca ggatactaca gaaaaaacaa tcaggaattg
    35821 tctcaatttt gaaaggagtt tacagcatga atagaataaa agtcgcaatc atcttcggcg
    35881 gttgctcgga ggaacatgat gtgtcggtaa aagccgcaat agaaattgct gcgaacattg
    35941 atacggaaaa attcgatccg cactacatcg gaattacaaa aaacggtgta tggaagctat
    36001 gcaagaagcc atgtacggaa tgggaagccg acagtctccc cgccatactc tccccggata
    36061 ggaaaacgca tgggctgctt gtcatgaaag aaagcgaata cgaaacacgg cgtattgatg
    36121 tggctttccc ggttttgcat ggcaaatgcg gggaggatgg tgcgatacag gggctgtttg
    36181 tattgtctgg tatcccctat gtgggctgtg atattcaaag ctccgcagct tgcatggaca
    36241 aatcactggc ctacattctt acaaaaaatg cgggcatcgc cgttcccgaa tttcaaatga
    36301 ttgataaagg tgacaagccg gaggcgggtg cgcttaccta ccctgtcttt gtgaagccgg
    36361 cacggtcagg ttcgtccttt ggcgtaacca aagtaaacgg tacggaagaa cttaacgctg
    36421 cgatagaagc ggcaggacaa tatgatggaa aaatcttaat tgagcaagcg atttcgggct
    36481 gtgaggtcgg gtgtgcggtc atggggaacg aggatgattt gattgtcggc gaagtggatc
    36541 aaatccggct gagccacggt atcttccgca tccatcagga aaacgagccg gaaaaaggct
    36601 cagaaaatgc gatgattaca gttcccgcag acattccggt cgaggaacga aatcgggtgc
    36661 aggaaacggc aaagaaagta tatcgggtgc ttggatgcag agggcttgcc cgtgttgatc
    36721 tttttttgca ggaggatggc ggcatcgttc taaatgaggt caataccctg cccggtttta
    36781 catcgtacag ccgctaccca cgtatgatgg ccgccgcagg aatcacgctt cctgcactga
    36841 ttgacagcct gattacattg gcgttaaaga ggtgataagt atggaaaatg gttttttgtt
    36901 tttagatgaa atgttgcatg gcgtccgttg ggatgccaag tacgccacat gggataactt
    36961 cacaggaaaa ccggtggatg ggtatgaggt gaatcgcatc atcggcacaa aggccgtggc
    37021 gtttgctctg cgcgaggcac aaatccatgc ggctgcgctt ggctatggct tgcttttatg
    37081 ggatggatat cggccaagaa ctgcggtgga ctgcttcctg cgttgggcag cgcaaccgga
    37141 ggacaatctc acaaaagaaa aattttaccc caatatagag cgagccgagt tgattacaaa
    37201 gggttatgtg gcctcacaat ccagccatag ccgtggaagc gcaattgatc ttacgctcta
    37261 ccacctggat acaggggaac ttgtttcaat gggaagtaac ttcgatttta tggacgaacg
    37321 gtcgcatcat acagcaaaag ggatagggga tgcagaggca caaaatcgaa gatgcttgcg
    37381 taaaatcatg gaaagcagcg gatttcagtc ttatcgcttt gaatggtggc actataagtt
    37441 gattgatgag ccataccccg atacctattt taattttgcc gtttcataat gaaagtattg
    37501 gttttgtatc agattttgga tttgataaaa tctatgctgg tcatggacaa tcttttactg
    37561 gaaagtggtt aaagcatctt ttgtaataca ttgcaaatta tagtattcaa tctgcccagc
    37621 ggcagaaaag aaaaaaaccg ctgttgggca gacctttccc acgcccattt tgtattttgc
    37681 tggcggcggt tttgagaaat caaggccgcc gttcttttct ggcagcaaaa ggtgtggcgg
    37741 cccataggag gatgccgcta tgctgttttg ctattgccat gctctaatcc acttccacca
    37801 ctcaaaaaaa gccttgcccc tc
//
LOCUS       DANKHJ010000022        37069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000022 DANKHJ010000000
VERSION     DANKHJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(11..1594)
                     /locus_tag="PW113_001221"
     CDS             complement(11..1594)
                     /locus_tag="PW113_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2525546.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2172..2315)
                     /locus_tag="PW113_001222"
     CDS             complement(2172..2315)
                     /locus_tag="PW113_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2525547.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="PW113_001223"
     CDS             complement(2538..3017)
                     /locus_tag="PW113_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2525548.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="PW113_001224"
     CDS             complement(3090..3374)
                     /locus_tag="PW113_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525549.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="PW113_001225"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PW113_001225"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="PW113_001226"
     CDS             complement(3718..5004)
                     /locus_tag="PW113_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2525550.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="PW113_001227"
     CDS             5142..5813
                     /locus_tag="PW113_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2525551.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="PW113_001228"
     CDS             5779..6267
                     /locus_tag="PW113_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2525552.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="PW113_001229"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="PW113_001229"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2525553.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="PW113_001230"
     CDS             7228..7992
                     /locus_tag="PW113_001230"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2525554.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="PW113_001231"
     CDS             8225..10438
                     /locus_tag="PW113_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2525555.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /locus_tag="PW113_001232"
     CDS             10449..10895
                     /locus_tag="PW113_001232"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2525556.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="PW113_001233"
     CDS             11066..12325
                     /locus_tag="PW113_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2525557.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="PW113_001234"
     CDS             complement(12414..13361)
                     /locus_tag="PW113_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2525558.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /locus_tag="PW113_001235"
     CDS             13755..15047
                     /locus_tag="PW113_001235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2525559.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="PW113_001236"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="PW113_001236"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2525560.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="PW113_001237"
     CDS             16940..17815
                     /locus_tag="PW113_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2525561.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18081..19964
                     /locus_tag="PW113_001238"
     CDS             18081..19964
                     /locus_tag="PW113_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2525562.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20039..20323)
                     /locus_tag="PW113_001239"
     CDS             complement(20039..20323)
                     /locus_tag="PW113_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2525563.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20313..21047)
                     /locus_tag="PW113_001240"
     CDS             complement(20313..21047)
                     /locus_tag="PW113_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2525564.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21176..21799
                     /locus_tag="PW113_001241"
     CDS             21176..21799
                     /locus_tag="PW113_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2525565.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21991..23112
                     /locus_tag="PW113_001242"
     CDS             21991..23112
                     /locus_tag="PW113_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2525566.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23119..26247
                     /locus_tag="PW113_001243"
     CDS             23119..26247
                     /locus_tag="PW113_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2525567.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26266..29460
                     /locus_tag="PW113_001244"
     CDS             26266..29460
                     /locus_tag="PW113_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2525568.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29606..30313)
                     /locus_tag="PW113_001245"
     CDS             complement(29606..30313)
                     /locus_tag="PW113_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2525569.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            31078..31476
                     /gene="spxA"
                     /locus_tag="PW113_001246"
     CDS             31078..31476
                     /gene="spxA"
                     /locus_tag="PW113_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2525570.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            31808..32461
                     /locus_tag="PW113_001247"
     CDS             31808..32461
                     /locus_tag="PW113_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2525571.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            32687..33685
                     /locus_tag="PW113_001248"
     CDS             32687..33685
                     /locus_tag="PW113_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2525572.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            33861..35669
                     /gene="pepF"
                     /locus_tag="PW113_001249"
     CDS             33861..35669
                     /gene="pepF"
                     /locus_tag="PW113_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2525573.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            35874..>37069
                     /locus_tag="PW113_001250"
     CDS             35874..>37069
                     /locus_tag="PW113_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2525574.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNN"
BASE COUNT        12181 a         6599 c         7798 g        10491 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa
    18001 ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt
    18061 atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca
    18121 ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc
    18181 caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt
    18241 tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat
    18301 tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt
    18361 cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca
    18421 gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc
    18481 gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact
    18541 atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga
    18601 tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata
    18661 caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat
    18721 ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga
    18781 aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt
    18841 gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga
    18901 gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat
    18961 ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc
    19021 agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt
    19081 tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg
    19141 tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt
    19201 tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag
    19261 caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac
    19321 aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag
    19381 aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac
    19441 aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg
    19501 ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac
    19561 aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca
    19621 ctgttctgat cggactagga acgaatggct cattctcgga agctcagttt gatagtgtga
    19681 tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct
    19741 ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc
    19801 tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc
    19861 atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag
    19921 gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt
    19981 caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc
    20041 atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc
    20101 gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt
    20161 ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac
    20221 cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct
    20281 gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc
    20341 tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg
    20401 tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt
    20461 gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt
    20521 tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta
    20581 cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg
    20641 tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg
    20701 actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg
    20761 acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga
    20821 cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca
    20881 actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag
    20941 tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg
    21001 atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa
    21061 aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg
    21121 tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag agactatgtt
    21181 ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg
    21241 ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg
    21301 tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat
    21361 ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc
    21421 ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt
    21481 gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact
    21541 taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata
    21601 tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg
    21661 cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga
    21721 acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata
    21781 tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt
    21841 cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt
    21901 ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat
    21961 aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat
    22021 atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct
    22081 atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat
    22141 gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg
    22201 aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga
    22261 ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca
    22321 gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa
    22381 ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta
    22441 gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt
    22501 agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc
    22561 gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat
    22621 ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa
    22681 ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc
    22741 ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt
    22801 ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat
    22861 atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat
    22921 cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga
    22981 aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg
    23041 gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg
    23101 gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt
    23161 atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg
    23221 aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa
    23281 acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa
    23341 gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa
    23401 ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta
    23461 acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa
    23521 gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt
    23581 ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact
    23641 gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa
    23701 aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa
    23761 aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc
    23821 caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta
    23881 acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg
    23941 aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa
    24001 aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta
    24061 gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa
    24121 agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa
    24181 aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa
    24241 aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa
    24301 caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc
    24361 gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg
    24421 caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata
    24481 gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa
    24541 gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa
    24601 cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca
    24661 ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa
    24721 cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct
    24781 gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa
    24841 caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa
    24901 gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa
    24961 acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca
    25021 agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc
    25081 tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa
    25141 agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga
    25201 cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg
    25261 caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata
    25321 gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc
    25381 cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat
    25441 aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg
    25501 ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc
    25561 caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta
    25621 caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca
    25681 agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga
    25741 gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg
    25801 ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta
    25861 gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat
    25921 caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca
    25981 ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc
    26041 gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca
    26101 atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta
    26161 agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa
    26221 agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt
    26281 ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt
    26341 gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg
    26401 gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa
    26461 cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct
    26521 cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa
    26581 atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct
    26641 ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct
    26701 attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta
    26761 aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa
    26821 cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg
    26881 catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa
    26941 ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag
    27001 cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata
    27061 caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat
    27121 ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc
    27181 caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa
    27241 ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg
    27301 tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta
    27361 gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga
    27421 aaaatccaag ctcgagtcga tttcgtctat ggcgaagtca tctattcgac tgacccgaag
    27481 catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga
    27541 gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca
    27601 ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag
    27661 tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat
    27721 caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca
    27781 gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt
    27841 tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc
    27901 caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct
    27961 aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa
    28021 tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc
    28081 aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg
    28141 ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta
    28201 cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc
    28261 gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct
    28321 ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac
    28381 acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg
    28441 caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa
    28501 actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca
    28561 atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc
    28621 ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg
    28681 tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg
    28741 aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa
    28801 atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata
    28861 cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat
    28921 taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag
    28981 aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa
    29041 gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat
    29101 cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta
    29161 aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt
    29221 tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa
    29281 aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca
    29341 ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg
    29401 acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa
    29461 gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta
    29521 tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc
    29581 atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct
    29641 gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat
    29701 ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat
    29761 tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca gctagctgaa
    29821 aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag
    29881 gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat
    29941 ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct
    30001 cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat
    30061 cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc
    30121 gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat
    30181 ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt
    30241 tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa
    30301 ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg
    30361 actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata
    30421 tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg
    30481 aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat
    30541 tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag tctcaatata
    30601 actattcaaa cataaaataa cgttatttga tttatttgtt tatagtaaat tttatttagt
    30661 aatcattgct ttaaaatact acgcttatat aacagagaaa ataagcgtta taataaagaa
    30721 cagtattatt taaaatgaca gaaaacatta ctattaaaga aataaaaaac ataaaaaatc
    30781 ttaagaaatc ggtttcattt agcttttgag ataaaataat agtacagttt tatacgctgt
    30841 ttcatatgtt tagaaaattt gaattagatt tgaaaactct aatttttaaa tgataaaaaa
    30901 agcgagaatg ctggttttac tgtatattaa ccttagataa ctttcatttt tttaaaaaaa
    30961 ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca gatgggagat
    31021 tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg gtgcacgttg
    31081 ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc gtggttgcaa
    31141 gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa cagttctgaa
    31201 ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc gacacgttcc
    31261 aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga attattggaa
    31321 cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga taaacgtctg
    31381 caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt acgacaatta
    31441 gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa acttggaaaa
    31501 gactattaca aagatagttt ttccaagttt ttttgttttt acaaaaagta ttcttgatta
    31561 gatgtataaa aaggtagtag aagatgatga ttgcacgaaa aatagagagg gaaaattagc
    31621 tgaaaagtcg gctatatagt attcgtctgc caaaaatgtt atagttaaat aagatagctt
    31681 gaaaggttga aaaatccgct ggttttatta aattcgctta acagcgagat tttttttctt
    31741 tacatttaag ctgatttcgc tacaatgaat ctatattaaa cattgtggca aagtgaggtg
    31801 tagcaaaatg gaaatggaac atattaatga aaatacgatc cgtgtactaa ttggtcatga
    31861 agatttagag gaacggggcg tttcattttt ggatttactt ggcaatcaca aagaaatcga
    31921 aaatttcttt tatagtattt tggaagaagt tgatatcgaa gaggagttcc gaggaagcga
    31981 agctgtcact ttccaagttt tgccaaaagg agacggatta gaactgttta tcagtaaaaa
    32041 tcttccggat gaagaaattt ctcaatcaga agattctact gttgattcag aagatgtgag
    32101 tgactttctt cgtcagcaga ttattggcag cgatccggag gaagaattat ctggaagcgc
    32161 cctagaagaa caggaaagac aagcaacttt tgcttttgaa gattttgaac aagtgatcca
    32221 acttgcaaca gatgtcgaac tagagtctgc ttggactgat ttgtatttat tcgaagagcg
    32281 ctattaccta gtcgttcact tctggatgga aaatctcaac caagcggatg tagagaatca
    32341 gattgctcga attttggagt ttgctgaaaa gagcgatcgt acaccagaaa cattgaatga
    32401 atatggacaa tgtctgatgg cgcgtaatgc tatcgagcga acgaaatttt attttgtata
    32461 aatcagcatt atagtaagag actgtgacaa aaattgtgtc gcagtctttt tttcgtataa
    32521 atgtgttcaa aagcttgcat ctgcggtaac aagcccaacc aaacgaaaaa tattgggaac
    32581 tatttttgtt tagctgttct tattacttga tgcagaatag ctttttcacg ccctcttttt
    32641 tcgtgtaaat aaattcttag acgtaaataa gttcagaggg agaaaaatgc tgcttgcaat
    32701 gaatgaatat aataagtgct gttttgctaa acatgcagaa aaaggaaaga aatattattg
    32761 tcgtggctgt cgaaaaagag tgattttgaa aaagggaaag aaaaaatgtg ctcattttgc
    32821 ccatcaaaaa tcagataact gttcggtctt tagtgaaaga gaatcaaaag agcacttaca
    32881 gttaaaggaa tgcttcatgg attggctggg acagtcagta gaaccagtat ttttggaagc
    32941 ctatttgccg aggttgcggc aaaggccgga tattttgttg gcgaatctag caatagaaat
    33001 ccaatgttct agactgtctc accagagatt tattgaaaga acccaaaatt atttgaataa
    33061 cagttaccaa gtatggtgga tacttggtaa ttcttttctt ggacaatcgc aattctcatt
    33121 gatagaaaaa agttgctgct actataatag aaaaagagga gtgcattgct ggaaagctga
    33181 tctcaagaaa caaaagcttt atttgtatca tcatattaca gagactgtat cagggcatat
    33241 ttccttttgc agtagctgct ggactttttc tagtagggac cttaaagaaa tattcactag
    33301 taacgaaatt aaaatgaacc aaatgaaaaa aatagaaaaa gtgagtgaag atgggaagaa
    33361 atggctgaca cgccaactta ttcacaagca aaagaataca gtatccatac aagaacaatg
    33421 ctacttgcgt cataaacatc ttttgcatct atcatcgtgg atctatcaaa aaagtcggtt
    33481 tttcttttat ttacaagaac aggtttttct ttatcgaatg ttgtacgaag aagccttgga
    33541 aaaccagaaa gttcctgatt tcaactcatg gctttgccaa ataaaagaac ataaaaggga
    33601 atggcttttt cctatgattg atgaagagat ggtctatcag cagtttttta acgaatgcat
    33661 acacttgtct tctttgaaaa aatagtaagt ataaaaatag gctaggaatc ggttttctat
    33721 gtaaaacaag atttagttat gctaaaattg atggaagcag acacatgaat gagtagaaaa
    33781 gttagatgct gtgacatgtg gatgttagac tatgatctat tttgatagat agagctaagg
    33841 gaagaaaagg agtggatttc atggaagtaa agcagttgcc aaaacgagaa gaattgcctg
    33901 aaaatttaac ttgggacttg accaagatct tttcaagcga ccaagagttt gatgagaaat
    33961 atttggaatt atcagaagag ttaaaacaat ctgaaaaaca caaaggaaca cttgatcaag
    34021 gcgcttctca atttttaaat gcgattgaat tcgtattgag ggtttatcgc caaactgaag
    34081 tcatttatgt atatgcgcac cttaaaaacg atcaagacac tggaaataca gattaccaag
    34141 cgctttatgc aagagcaagc agtctgtttt cgaaagttag tgaagccgtt tcctggtttg
    34201 aaccagaaat attgcaattg tcagatgacc agatttggca atatttcaaa gaagaaccaa
    34261 aattggaagt ctatcgccat tatatccagc aaatagtaga taatcgagcc catgtcttat
    34321 ctgctgagca ggaatctctt cttgctggag caggtgaaat ctttgatgct tcaagtgata
    34381 catttgctgt tttgaataat gcagatctag tttttccaac gattgaagga gaaaatggtg
    34441 aaatagtcca attatctcat ggcgtgtatg gtcagttgct agaaagcacg gatcgaaggg
    34501 tgcgcgaagc agcatttaag gggttgtaca gtgtttacga acaatttaga aatacatttg
    34561 cttctacttt aggcacacat ataaaaggac ataattttaa agcgaaagtc cgtaattaca
    34621 gctctgccag agaagcgtct ttgagcaata atcatattcc tgaaagtgta tacgatactt
    34681 tggtagacgt ggtaaacaag catttgcctt tgttacatcg atacatggaa ttacggaaac
    34741 gtttattaga agtggaaaaa ctgcacatgt atgatcttta tacaccggtc ttaggggaag
    34801 ctccaattac ctttacgtac gaagaagcaa aagaaaaagc tttagaagca ctgaaaccaa
    34861 tgggtgaaga atacatggcc atcgtagaaa aagcattctc tgaacgttgg atcgatgttg
    34921 tcgaaaataa agggaaacga agcggtgctt attcttcggg aagctatgac acaaatccat
    34981 atattttatt gaattggcat gatacgctgg atcagctatt tacgcttgtc cacgaaatgg
    35041 gacatagtgt tcatagttat ttcactcgtt cgaaccagcc ttatgtgtac ggcgactact
    35101 ccatcttttt agcagaaatt gcttcgacaa cgaatgaaaa tatcctaacg gagtatttat
    35161 tggaaacaga aaaagaccct cgtgtacggg cttatgtact caaccattac ttggatgggt
    35221 ttaaaggaac agttttccgc cagacacaat tcgctgaatt cgagcatttc atgcatacgg
    35281 aagatgaaaa aggtgtgcca ttgactagtg aatacctaag tgatagttat ggtaaattga
    35341 atgcaaaata ttatggtcca gcagtcgaag aagacccaga aattaaattc gaatggtcac
    35401 ggataccgca tttttattat aattactatg ttttccaata ctcgactggc ttttctgctg
    35461 cttcagcact ggcgaagaaa atactaaacc aggaaccaga ggcactagag aactacttgg
    35521 cttacctaaa agcaggtaac agcgattatc ctgtggaagt aatgaaaaaa gcaggagttg
    35581 atatgacaca ggctgcatat attgaagatg caatgtcgat gtttgaacaa cgtctaaatg
    35641 aattagaaga attgatcgat cgtttataag gctttctatt tttgattttc tcgaatagaa
    35701 acgaaaaaaa tacgtcggct ctcttgtaag ggagtcgacg tattttttta aggctctata
    35761 ctttttgatg ttggtgaact tcaccaacat cttttttgta taaaaaaata tagaaaagac
    35821 accctgaaat cctttagaat taatttggct accaattcat aggaggattc aagatgtctc
    35881 atactcagct tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa
    35941 attgtttaac taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc
    36001 tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt
    36061 gggggacgac aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg
    36121 agctgaagaa acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct
    36181 ctgttgctga aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct
    36241 taaaagagaa tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg
    36301 tctatcgtgt catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa
    36361 aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta
    36421 tcatgatgga cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt
    36481 atttagaacg ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg
    36541 ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt
    36601 tgatcatcga tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag
    36661 tgaaagaaac cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa
    36721 aattgaagcg ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac
    36781 gggtttggcg tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt
    36841 tagctgcttg tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg
    36901 taaagaaaag agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc
    36961 cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg
    37021 cgcttcacta cagctattca aacggaccat tggaatgctt aaacaacca
//
LOCUS       DANKHJ010000023        35432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000023 DANKHJ010000000
VERSION     DANKHJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            600..1469
                     /locus_tag="PW113_001251"
     CDS             600..1469
                     /locus_tag="PW113_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2525575.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1678..2703
                     /locus_tag="PW113_001252"
     CDS             1678..2703
                     /locus_tag="PW113_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2525576.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2742..3692
                     /gene="manA"
                     /locus_tag="PW113_001253"
     CDS             2742..3692
                     /gene="manA"
                     /locus_tag="PW113_001253"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2525577.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3786..5489
                     /locus_tag="PW113_001254"
     CDS             3786..5489
                     /locus_tag="PW113_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2525578.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5491..7524
                     /locus_tag="PW113_001255"
     CDS             5491..7524
                     /locus_tag="PW113_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2525579.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7535..7975
                     /locus_tag="PW113_001256"
     CDS             7535..7975
                     /locus_tag="PW113_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2525580.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(8175..9302)
                     /locus_tag="PW113_001257"
     CDS             complement(8175..9302)
                     /locus_tag="PW113_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525581.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9305..9982)
                     /locus_tag="PW113_001258"
     CDS             complement(9305..9982)
                     /locus_tag="PW113_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525582.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            10246..12012
                     /locus_tag="PW113_001259"
     CDS             10246..12012
                     /locus_tag="PW113_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2525583.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            12027..12464
                     /locus_tag="PW113_001260"
     CDS             12027..12464
                     /locus_tag="PW113_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2525584.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12477..13628
                     /locus_tag="PW113_001261"
     CDS             12477..13628
                     /locus_tag="PW113_001261"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2525585.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13690..14388)
                     /locus_tag="PW113_001262"
     CDS             complement(13690..14388)
                     /locus_tag="PW113_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2525586.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14381..15733)
                     /locus_tag="PW113_001263"
     CDS             complement(14381..15733)
                     /locus_tag="PW113_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2525587.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15956..17356)
                     /locus_tag="PW113_001264"
     CDS             complement(15956..17356)
                     /locus_tag="PW113_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2525588.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17517..18254
                     /locus_tag="PW113_001265"
     CDS             17517..18254
                     /locus_tag="PW113_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2525589.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18379..18858
                     /locus_tag="PW113_001266"
     CDS             18379..18858
                     /locus_tag="PW113_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2525590.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18855..19622
                     /locus_tag="PW113_001267"
     CDS             18855..19622
                     /locus_tag="PW113_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525591.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19852..20421
                     /gene="lepB"
                     /locus_tag="PW113_001268"
     CDS             19852..20421
                     /gene="lepB"
                     /locus_tag="PW113_001268"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2525592.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20525..21127)
                     /locus_tag="PW113_001269"
     CDS             complement(20525..21127)
                     /locus_tag="PW113_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2525593.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            21233..21702
                     /locus_tag="PW113_001270"
                     /pseudo
     CDS             21233..21702
                     /locus_tag="PW113_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21808..22392
                     /locus_tag="PW113_001271"
     CDS             21808..22392
                     /locus_tag="PW113_001271"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2525594.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22395..23468
                     /gene="prfA"
                     /locus_tag="PW113_001272"
     CDS             22395..23468
                     /gene="prfA"
                     /locus_tag="PW113_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2525595.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23461..24300
                     /gene="prmC"
                     /locus_tag="PW113_001273"
     CDS             23461..24300
                     /gene="prmC"
                     /locus_tag="PW113_001273"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2525596.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24293..24472
                     /locus_tag="PW113_001274"
     CDS             24293..24472
                     /locus_tag="PW113_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525597.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24494..25519
                     /locus_tag="PW113_001275"
     CDS             24494..25519
                     /locus_tag="PW113_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2525598.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25710..26954
                     /locus_tag="PW113_001276"
     CDS             25710..26954
                     /locus_tag="PW113_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2525599.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            27107..28054
                     /locus_tag="PW113_001277"
     CDS             27107..28054
                     /locus_tag="PW113_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2525600.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            28188..28817
                     /gene="upp"
                     /locus_tag="PW113_001278"
     CDS             28188..28817
                     /gene="upp"
                     /locus_tag="PW113_001278"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2525601.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28956..29198)
                     /locus_tag="PW113_001279"
     CDS             complement(28956..29198)
                     /locus_tag="PW113_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525602.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29319..29391
                     /locus_tag="PW113_001280"
     tRNA            29319..29391
                     /locus_tag="PW113_001280"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29352..29354,aa:Thr,seq:cgt)
     gene            complement(29510..30148)
                     /locus_tag="PW113_001281"
     CDS             complement(29510..30148)
                     /locus_tag="PW113_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2525603.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(30271..30903)
                     /locus_tag="PW113_001282"
     CDS             complement(30271..30903)
                     /locus_tag="PW113_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2525604.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            31254..31553
                     /locus_tag="PW113_001283"
     CDS             31254..31553
                     /locus_tag="PW113_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2525605.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            32048..34213
                     /gene="acm"
                     /locus_tag="PW113_001284"
     CDS             32048..34213
                     /gene="acm"
                     /locus_tag="PW113_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2525606.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34485..34970)
                     /locus_tag="PW113_001285"
     CDS             complement(34485..34970)
                     /locus_tag="PW113_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2525607.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    35060..35405
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        12185 a         6106 c         7094 g        10047 t
ORIGIN      
        1 tcaaaaaacg gcaaaatatg agaagctatt tttccgtttt gtatctctaa tactcaggtc
       61 aaaccgctga ttttacaacc tcttccttaa acggtgttct cctgccaact cctcggtcac
      121 aagtcacaat agtcaacaaa cctgagaagc tgttttttga aattgctcct tgtgactcgg
      181 agttaaacgg caggctcaca acctcttggt aaacggtgtt caaaagccag tttctcggta
      241 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc
      301 gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc
      361 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
      421 acttgatgca gaacagcttt ttcacaacct ctttttataa gagtttttac tcattaattt
      481 acaaaaaaca agcaaaatgc gaattttctc acattttttt taacaaaatc aaagttattg
      541 cttgaaagaa gaagaatcca tcgttaaaat aaagcttgac gtagagggga gcaagaaata
      601 tgaaagattt tcaattgggg gctgccacct atttaaaaag tgtagccatt cgggtgaaag
      661 accgagataa aatgattgat ttttacaaga acgttgttgg ctttgatttg aaaagagaag
      721 aaaacgaatt agccatttta ggaacagaag aagctggaag tgaaatgttg ctgctggaag
      781 aaagtcctag agcgaatgat cacatggggg tgatcaaaaa gctgaaccgt ttttcattga
      841 ttatccctag cacagaagaa atggcagata tcctttgtcg tatccgtaaa atgaattatc
      901 caatcaaaga agctttagaa gatcatggac gtatggggat tttgctcgta gacccagaag
      961 agaatgaatt ggaaatttac catgaagaga ataagaacga ccaaagagaa attccagaaa
     1021 caatggatca agaagcatta ttaagaaaat ctgaagaaaa attcagtaaa ctatcaacag
     1081 gttcttattt tgaaaaaatc catttaaatg ttactaactt aacaaaagaa cgccaatttc
     1141 ttaatcatgt acttggtttg aaggtacata atgaagaaga gggcatccat gtattgaatg
     1201 aaggagattt ccatgttggt ttgacagaag ctagcggagg cgcaattgat ttgcctacag
     1261 atgaagtgat tggacttgat tttctgaaat tcatgatcga tactgaaagc atggaaggac
     1321 tgattgctca cttgcagcaa ctatcagttg atttcttctt agacaaaaag cacacagtac
     1381 tcacaatcta tgatgctata ggaatcgaat ggtggttcgt cagtgagcca aaagaaaaga
     1441 ataaaactga cattcgatgc gacgcttaac tttttctttc ttaacgattg tcgcatgtct
     1501 cgtcatttta tgaacggtca tactatcgct tattctagag atagaaagag aaaatagtat
     1561 accgggggaa actgcgcggt ttttcgcgca gtttttttgt gtaaaaaagg ttgattcctc
     1621 atagattaag ctttaagctt ggactgtaag aatctgatag aaaagcagga gaaaaagatg
     1681 tatccacaca cgaaacagct tattttgaaa aagatggaag aaggatttta tccaggtgta
     1741 gcagtcgcct tcataaaaga aaatcaagta gaaacattta cagccggctt tgcccaagtc
     1801 gttcctgaaa agcagctgat gacagaagat ctgttgtttg atgcagcctc actgacgaaa
     1861 gtcgttgcaa ccacttcatt agttttacgt ctattagaag atggaaagat agaactcgat
     1921 caaccgcttc ataaatatct tcctgaattt gaaaatcaga caatcacttt gcgtcattta
     1981 ctgacacata cttctgatat caaaacatgg ataccaaaca gagataagct atctcaggaa
     2041 gaattgatcg ccgcttattt gaagctagaa ccaggtgagc aactaggaaa agaagtaaaa
     2101 tatacagatg ccggtacgat tcttcttggg ttgatgctag aggaaattta ccaagaaact
     2161 gcagtggaaa tctttcaaga acaagtattg gaaccattag gaatgatgaa cagcttattt
     2221 cttccttttc cttccaaaaa aatcgttccc acagaacaac tgccttcggg tgaggttttg
     2281 cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg aacatgcagg taacgcgggt
     2341 ctctttattg atttgaaaga cagcattact tttgtccaaa tgtatttgaa caagggaaaa
     2401 atccgtaatg gtcgtttttt agaagaagag acgattgatt ctttactgca agatcaaaca
     2461 ccgaaaaaaa atggctatcg ttctttagga tgggacttaa aaaaggatgt agaagacggt
     2521 catgcgattc tttttcatac tggttataca ggtacgtttt tcttgattga tataaaaaca
     2581 caagaagcgt tgatattttt gtcaaatcgc gtacatcctg tcgatcatcg tgcggagtat
     2641 ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag agaaagcggt attaccgaaa
     2701 taatgctata atagctatga aaaatgagag gagtttgaat tatgcaggaa cctatgtttt
     2761 taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg tttacgttca gtctttggtt
     2821 ttgatatccc taacgataaa atcggagaag attgggcgat cagtgcgcat cctcatggtg
     2881 tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt agatgaacta tggtcagaac
     2941 acaaggaatt gtttggccat ccttcagaac cagtgtttcc attattgatc aagatcttag
     3001 acgcagaaga tgatctatct gtacaagtac atccagatga tgcctatggg atgaaacatg
     3061 aaggtgaatt aggaaaaact gaatgctggt atatcatcga tgctgaacct ggtgcagaaa
     3121 tcatctatgg tcatcatgca aagacaagag aagaactagc tgagatgatc aaagatgggc
     3181 gctgggatga ccttttgaag aaagtaccag ttaaaaaagg tgatttcttt tatgtaccaa
     3241 gcggaacgat ccatgctatt ggtaaaggaa tcatgatttt agaaacacag caaagttctg
     3301 acacgactta ccgcgtgtat gactacgaca gaaaagatgc gcaaggacaa acaagagagc
     3361 tgcatatcca acaatcgatc gatgtaacaa ctgtccctgc gaaaacacca gaattacaaa
     3421 tcaaagaagt ccgaaaaggg aattcatcga tcgtcactta tttagaaact gagtttttca
     3481 atgtatatga gtgggatatt aaaggaatca catctttcaa aaaacaagcc ccttatacat
     3541 tgatgacggt aatcgaagga gcaggagacc ttgtcgttga cggaaaaaca tatccattag
     3601 aaaaaggaac aagctgtatt attccaaatg gcgtttctga gtgggcggtc caaggtgaac
     3661 tagcaatcat tgcgtctgaa ccaggcaaat agttcccaga tagctgaaag cagcagacgg
     3721 tttttcgttt gctgcttttt ctacataaaa tccgtccatc aataaataga agtctggtga
     3781 aaaagatgga atacaaatgg aaagcaaaca acagcagtcg cgtgtctatg aaacaactga
     3841 gaaaagctgg acatcactta aatgaaatga tcatgccaaa tctaagtctg tttatcgctt
     3901 ggggaatctt gtcattagca acaaaaggtg tgtctggcga tttgcacacc acattagaag
     3961 aggtatgcca ttggatgatc cagctgcttt tacccatctt gatcagttat tcaggcggga
     4021 aatcggtagg gggacaaaaa ggggcagtcg ccggtgcaat tgcttcttta gggttgatcg
     4081 ctcgttccaa tgctccacaa atcgtaggag cgatgattat cgggccgctt gccggcatcg
     4141 tatatgaaca atttgtttct agattccatg tgaaattcaa ggctggatat gaaatgcttg
     4201 tcagcaacct actagtagga ctgactggta gcgttatttg tataattgga atcgttttta
     4261 ttgaaccagt tttaaatagt gttcacttgt tgctcgcctc cgttgtgtct tggctagtca
     4321 caatgaatct gttaccactt gtcagtctga tcttagaacc tctaaaagta ctatttttca
     4381 ataatgcaat caatcatggt gtattgacac ctttaggaat cgaattctca caaacagtcg
     4441 ggtactcttc cttatttttg atggaggcaa atcctggacc tggtttaggg atcttacttt
     4501 ctatcctttg ttttgcagaa aaacaggaaa aagtaaatgc gagtggagca ttgatgatcc
     4561 atgcgattgg cgggattcac gaaatctatt ttccatttgt cttgctgcga ccgtatctat
     4621 ttttagctgt aatggctggt ggggcaagcg gcacgttgat atttcaatgg ttagatacgg
     4681 gattagctgc acctgtatcg cctgggtcac tactcgtgat ttttgccaat gcgccttatc
     4741 cacaactagc tggggtaagt atagggatag caatcagtac atttgtgacc ttcatttgtg
     4801 cagcgatact attgaaatta ccaaataaag aaaaagagaa caaaacggtc aaagaaaggg
     4861 gagaaatctt ggaaccatct atcattcatt ccgtcatttt tgcttgcgac tcaggtatgg
     4921 gatcaagtgc tatgggagcc gccctgctga aaaagaaatt agcagcagaa aaaatcgatc
     4981 tttctgtcgg atacagctct atctatgaat tgaataacca agtaaatcag ctggttatcg
     5041 tgcaacatga actagcgggt cttgcaagag aaaaagtacc tcaaagtcaa atctatgtac
     5101 tggatcactt tttggaaaca gatatcttgc tgtcacgaat aaaagaagcg atccaatttc
     5161 caaagcggga agaaaaaaaa gagatagcag atatcacaga acaaaaaacg tcgcctgcta
     5221 atattgtatt tttgtataaa aacaacagac gcggctcaca aactatgggc atgacgattt
     5281 taagtcaatt agcaaaaaaa gcgaaaaaac agtttgttat ctcaaaagaa tcaatagaaa
     5341 aaatcaaaat ccaagaagaa acgatttata tcgcacaaag agaactagct gtaactgaac
     5401 agctagaaga aaagatccct cggctagttc tagttgaaca ttgggtagct acgaatgaat
     5461 acgaaaaact cgtgaaggga gaaatataag atgtatttat cgcccagaga aaaacaatta
     5521 ttagcagaat ttctcagtag cccgtcacct gtatctatcc aaaaaatgat gaatttattg
     5581 aaggtcagta aacggactat ttaccgcgaa ttagataatc ttgaagtctc attgaaatcg
     5641 gtaaatgctt ctttgaaaaa agcagctaga ggctcatttg tcattgaggc atcagaagaa
     5701 gtattagagc aattaaaaga aaatacagat gggaaaagta atgacttatc cactgctaaa
     5761 cgacaacacg cgattttagc cgagttgtta ctgacgacag agccgttgag tcttgctcat
     5821 ttcatggaat catatcgcat cagtaatacc actttttatg cagatatcaa acagttggaa
     5881 gaaagtattc gccaactgcc tttggaaatc attcggaacc agggatacga gatcgaagga
     5941 ccggaaaaat accgccggct tctgacagcg aatgtattag aattagaaat caatgaatat
     6001 gaactttttc atagtatttc tttcgatgca tcactgaatt atttttttcg attcgttgat
     6061 ccgcaacatc tatctcttgc acgcaaagta atcggggaag aattgatcca aaaaaagacc
     6121 aatctaagtg atagaaagct tgaacatttg gttttgatgc tgacattgac gatggagcgt
     6181 gtgacgaaaa atcatttact gacaaatgag acatataata gtctggcaaa caaagaatta
     6241 ctacagacag ctaaacgatt gttttcacgg atcgcttctg agacaaaaca actttatcca
     6301 gtaaatgaga tcgtcttttt tgcaggatta ttaaatgact tcacgaactc atttgatcag
     6361 aatttctttg aagagacgtt cgatacacag cttgcttatt tagtaaaaca actgattcaa
     6421 gaagtaagtg aagagacgaa tgtccatttt tatgaagatg aaactttgta caaaatgctg
     6481 ttgactcatt tatctggtgt gttttcaaga gccgttttac aagaagagac actgaccaat
     6541 ccgattttag aaaaaatcat ggttcaatac gaggaagttg cgaatgcaat ccgtaaagca
     6601 gtagagcgga tttttcaaga aaaaaaatta tccgaagaag agatcgctta tatggtcctt
     6661 cattttgcga attcattgga acgaagtccg aaagaaagca atatcagtat tgcaggattt
     6721 tcgccaagtg gattagcttc tactagcatg ttggaactac gattgaaaag gtattttccc
     6781 ttcatccaac aaattcattt ctatcggatt gctgatttaa aaaaaatcga tttacaagaa
     6841 gaatatgatg ttgttatttc tacatctatt ttaccaggat acggtggaaa atatatttta
     6901 gtctccccgc tactgctaga agatgaagtc aagcagctaa aagaagcttt ttcgcatatt
     6961 gagaagaaaa aaaggcaaaa aattacgtat aacagcaata acgagtggat gatagaagat
     7021 acttatgagg aaacaatgca tttcatggat caaatcaatc aattactcgg tcgttttttt
     7081 atccaagaac tggataatcc tgagacgatt tccgaaacga ttcgcagctt atttccttat
     7141 tttcctgaag gactagtaac ggatgaagag gtggttcatc gccggctgct taagcgatat
     7201 gaacaagcac ctatcggtat tcctcataca aatatggggt tattccatac atccagctca
     7261 ttgatttcta aaccgatctt ttgtattttc aatttgaaac atccgttgac cattgaaggg
     7321 atggatagac agccagtcga tttgtcccgt atgttagtta tgctggcgcc ttcgccaatc
     7381 caagaaaaag acgggattct tttaggaaag atcagtggag caatcatcat gaatgacttg
     7441 aacaccgaga tcttccgctc aggaaataaa gaaatcgttt atcagttgtt agcgaaaatc
     7501 ctgattgaag aaataaagaa atgaaggtga aaagatgata cagctaccag cagaaaaaat
     7561 attgctcgat caatcctatc aaaattggaa agaagcgttg accgagatcg gccagtggat
     7621 gacccaacaa gggggaatca aaccggcata tatccaatca atgatcgaaa gacaagaaaa
     7681 agcgagtgtc tatatcggga atttcgtagc cttgccacat gggactgaca aagatgaaga
     7741 agtagtagaa gaaggaatct atttagtcca agtgcctgat ggtgtggact ttggaacaaa
     7801 agaggaacca aaaatcgcga cactcttatt tgtagtggct ttaaaaaaag aacatcagtt
     7861 agcggtgctc caagatttag cctttttctg ttcagacatc gaccaagtaa tggcgctcag
     7921 tgatgcggaa accattgaag aagtccaaat gatttttgaa catggacata tataaccaat
     7981 gttcacagta aaaaaagctg ccttgcatca agtaataact tcaaccaaga taaaaataag
     8041 aggagctatt tttgttggga ggttcttatt actgcggatg caagcttttg aaaactattt
     8101 attctagaaa accgtttatt tgaaataggg gacggtgaca aggtttttgt caccgcccct
     8161 attatttttg tgttttaata gctctttttt atatagatag tgcccatacc tatgaataat
     8221 aaaacgatac tccatatacc aagtgtcaag ataccattca tgaaagtaac ctgttctgaa
     8281 gctagtggcg agaaaatatc tcctcctatg atcacatttt gaaaatcaac ataagaagtt
     8341 ggcaagtaat gactgattga ttctatccat ccaatcgaag aaaacaagcg atactctgct
     8401 aagaaaagac aaagaagtgt acctaaaaga ttgactagag aatgttcaaa taaagcagat
     8461 agaagaaaac tgattgtact taaaaagagc acccacaaca acaggaacag aaagctctgg
     8521 aaaagatact catacatcgg caggattcgt atctcctgac caatgatcgt tcctacagga
     8581 taacggaagt ctcccaaacc atttttgatt attactagta tagagacgat caaaaatggc
     8641 atcacgagac ttagcatagc tgcaccataa taaatggtaa aacggatgct atgtttcttt
     8701 accatactat ccagtaatag attgactaga gagattacct gctttctttt ttcataagag
     8761 atgatggaag aaagaaacaa ggcggttata cataaaatca aaaaactaga aatcattcct
     8821 gaaaaaatat tttcgtagta atttgctaaa gagagatgcg ctggcaagtc aaaaatagaa
     8881 tatctttgga tctgatgttc tttcatataa cggagcaact cgacacgttt ttgttgttca
     8941 atgattggaa ttccttgtct ttgaccagaa atcagtcgat ccagtatatc tttttcataa
     9001 tgataagttg cttcgaccat ttgctggaca ttccctgtgt tttttgcatg taagattgct
     9061 tcgatcagtt tgttgctttt ctctaaactt tccacaaatt ctttctcatt ctcatcttct
     9121 ttcaacgttt caatcaaacg ttgcatctct tcatagttat gattcagttc atcgaattct
     9181 gtataagcaa gtttatgatc aaaaaaaagt gggaatgcaa agagcacgac caacaataga
     9241 ggaaataaaa agtttacttt gttttttagt aagagattgc gttcaaatgt aagtaaagac
     9301 atagttacac ctcatataat ttacgataaa tagctaaaat ctcttccata tttgaggatt
     9361 ctgtgacgat ttctccattt tttagaaaac atgctttaga acaaatcgat tggatattcg
     9421 cgatagcgtg ggaagaaaga agaatcgtct tcccctcttt ctgccattgt ctgatgaggc
     9481 tattcaagat atcaatactt cctgggtcta atccgttcaa cggttcatcg aataacatga
     9541 cagatgaatc actgatcaag taagcagcaa gcaagacatg ttgtttcatt cctagagaca
     9601 tttttccaat cggcaaagta aggtactcag tcatcttcaa tcgttggaaa acagtttggc
     9661 attctacttt gctctgccaa tagtatttga tcaaagtcaa ataatcaaca gccgatagat
     9721 agtcggataa ctgattcgtt gattctagaa aaaagaaaga ccggcgataa tctgtataag
     9781 cagtaggaga gatgttgtga agggacaatt gatcataagt tgttggcagc agttgggcaa
     9841 tgccattcag caaagttgtt ttgccaaatc cgttcggggc aatcaaagca gctacttctc
     9901 ctgattctac taaaagattc aattgtcgaa tcacacgttt tttaccacgt ttcatgttca
     9961 tgtttttgat cagtaatgtc atagataggc ctccaatata aaatttaata acataataga
    10021 atggaaaaca tctaaattaa atgctaacaa aagttattgt ttgcatatta taaaaaaatt
    10081 aaaataacag ttttatttaa aattgaagtt ttctgatacc gaaatctttt tattttgctg
    10141 acatactcaa acctttggca caaagtttcg tgccaaagta cgactatttc tctttttagt
    10201 aagcggatac aattagagta tcaaaaggag agagggagag acaaaatgga acggacgatt
    10261 cccaaaaaca acatttcgct aaaagcgagg gtacaaaaat taggcagtac tttatcaagc
    10321 atggtcatgc caaatattgg agcactgatt gcctggggtg tattaacggc tctatttatc
    10381 ccagatggtt atttaccaaa tgaggctttc gctaccatgg taagcccaat gctaacttat
    10441 ctgattcctt tattgatcgg ttatactggc ggaaaagtaa ttgctggtga ccgcggatca
    10501 gttgttgggg caattgcaac gatgggtgtg attgtcggaa cagatattcc aatgatgcta
    10561 ggcgcgatga tcatgggacc tcttggtgga tatgccatca agaaatttga tcagctattc
    10621 caaaaacgga tcaaatccgg ttttgaaatg ctagtcaata atttttcagc aggtcttatc
    10681 ggttttggat tagcgttatt aggtttttct gcgattggtc cagttgtcga tgcattgact
    10741 caagcaatgg caaaaggtgt agagatcatt ttatctgccc atttgattcc attgacaagt
    10801 atttttattg aaccagcaaa aatcttattt ttgaataatg caatcaatca tgggattttg
    10861 acaccattgg gaactgaaca agtagcagag gctggaaaat ctattctatt cttgcttgaa
    10921 gcaaatcctg gtccaggact tggcgtatta cttgctttta ctttgtttgg caaaggagca
    10981 gcaaaatctt cagcaccagg agccatgatc attcactttt taggcgggat ccatgaaatc
    11041 tattttcctt acgttatgat gaaaccgtta ttattcttat cagttatagg cggaggaatg
    11101 tcaggaagtt tcattttcca attgatggat gctggactaa gagcgccagc ttctccaggg
    11161 tcgatcattg cgattctagc gatgacaccg ttcaattctt accttcctgt tattttgggc
    11221 gtgattgttg ccgcagctgt ttcgtttggt atttctgcag taattttgaa agcggatgcg
    11281 aaagatacaa atgaagactt cgaaaaatct gttcaagaga caaaggcagc taagcaagct
    11341 tccaaaggtg cccaaggtat tcctcaagga gctacattct ctggcgtaca aaaaatcatt
    11401 tttgcttgtg atgcaggaat gggatcaagt gcgatgggcg cttcgatctt gagaaaaaaa
    11461 gtacaggaag cagacttgcc tcaaacggtg accaatcaag caatcagcca actgacagat
    11521 gactcgaata cattgatcgt tactcaggtc gaactacaag aaagagcaaa acaaaaagca
    11581 ccgagcgcgc gttttgtctc agtagaaaac tttttgaatt cacctcgata tgatgaaatc
    11641 attcaagaat taagcagcaa gcagaaaaca tcagtttctg aaattgagaa tgagccagca
    11701 gaagagtttc tttcagcaaa catcaaacaa atcaaagaga ttctacttgt ccacgatgat
    11761 cgttccggat cagcaacaat ggggatgacc gtgctcaaag atatattaga caagaaccat
    11821 ttaactatcc cgttaagaaa agtcaatatc caagatttaa aagatgaacc tgattcactg
    11881 atcgtagcca aagaaagttt agcggaaaaa gtacagatgg ctgcacctca atccattcat
    11941 ttatcagtca acagcatggt caatccgcaa aaatacgaat cgattgcaca atatttgaaa
    12001 cagacagctt aagggaggaa aacagcatgc aaagtttgcc attcgacatg atcgagctca
    12061 atcgatcttt taaaaccaaa gaagaagcga tccgctattg cggtcagatg cttgtagaag
    12121 cagggtgcgt tgatgaggaa tatatcgatg ccatggtaga tagagacaaa atgttatctg
    12181 tttatatggg gaattttatc gcgattcctc atggaacaga tgaagcaaaa aaatatgtga
    12241 aaaaatccgg catctgtgtc gttcaagtac cagatggtgt agattttggt accgaagaag
    12301 aagaaaagat tgcgactgtt ttgttcggta ttgcaggagt cggagatgag catttgcaac
    12361 ttgtacagca aatcgcactt tattgcagtg atatggacaa tgttgtccag ctagcagacg
    12421 cgctcagcaa agaagaagta acggaaaatc tagcaattgc ttaaggagag agaaaaatga
    12481 gagcagtaca ttttggtgca ggaaatatcg gtcgaggatt tattggagag gttttggcag
    12541 ctaatggatt tgagattact ttcgtagatg tgaatcacac gatcattgat gctttaaatg
    12601 aaagaaaaga atacgaaatc gaattagcag atgaatcaaa agaaaaaatc actgtaaaaa
    12661 atgtcaaagg aatcaacaat caaacaaatc cagaaaaagt aatccaagaa atcgctgaag
    12721 cagatttagt tactaccgcc attggcccga atatcttacc ttttattgct gagttgattg
    12781 caaaaggtat ccaagaaaga gaaaaggaag gaagcacaac atctttagat atcattgcct
    12841 gtgaaaatat gattggcggt tcagcatttc tagaaaaaga agtgtataaa tatcttccgg
    12901 aaacatcatt tactgacaaa tatatcggct ttccaaatgc agcagtcgat cgaatcgtcc
    12961 cattacaaca ccatgaagat cctttattcg tccaagtgga acctttcaaa gaatgggtca
    13021 ttgacgatag ccaaagaaaa aataaaaaaa tccaattaaa aggggtttta tatgtagatg
    13081 atctagaacc atatatcgaa cgcaaactat ttagtgtcaa tactggtcat gcgactgtcg
    13141 cttatacagg tgccttgtta ggttaccaga ccattgatga agcaatgcaa gatgcgttag
    13201 ttgtggcaca attaaaatca gttttgcagg aaacaggaag tttgctgatt gccaaatggg
    13261 ggtttgatgc agagcagcat catgcgtata ttgaaaaaat cattcatcgc ttccaaaaca
    13321 aaaatatctc tgatgcgatc acacgtgtcg ctcggacacc attacgtaag ttaggttatc
    13381 aagaaagatt tacacgtcca gtgagagaac tacaagaaca taacttgact tgcccacatt
    13441 tgacggcaac aatggggatt atttttaatt actatgatcc tgaagatgaa caaagccgtc
    13501 aattgcatga aatgaagatc catgaaaact tggaacaatt gattcaagaa gtcacaggaa
    13561 tcaatgatcc gaaaacaatc gggaatatca agcaaaacgt gaaccgttat gccaagcaag
    13621 tcgcttaaaa taggatacga aaaaggggaa tgtgacgaaa tttttgtcac attccccttt
    13681 ttcgaataac taggctgttt gtccttcaag atagggcaga tccaattctt taccgtaacg
    13741 ttgttctaaa gctaggcgga tcaaacgagc tgccaggttc tcatttcttg caaggatagg
    13801 tccatggaaa taagaaccga agacatttcg gtaaatgaca ccttcagatt ggtcttcacc
    13861 attatttcct tgaccttgta ccactttccc caaaggtcgt tcaccttcac ctaagaaagt
    13921 ccgaccattg tggttttcaa atccataata agtttcattg aattcttcgt tatggataac
    13981 gatatcaccg ataaaccggt tattttcctg acttaacgtg tagtgatcca gtgcactgat
    14041 tccttggatc ttttctccat tagctcccat gtagtagtga cctagcaatt ggtagccacc
    14101 acaaatggct aacatgacgc catcattttc gatatagttg atcaaagaat ctttcttttt
    14161 ctggatgtct tttgaaatga ttaattgctc aaagtcttga ccaccgccaa agaaaaccaa
    14221 gtcatattta tcagcatcaa aaggttcatg tatactaacg atctccgtat ggcaagaaac
    14281 ccccattttt tctgctatat atttcaacat caaaaggttt ccgttatcac catatgtgtt
    14341 catcaaattt ccataaaggt gggcgatatt gagttcataa ttagccacgg ttcattcctc
    14401 ctttgataaa tccttgagca gctagttctt tacgaagctg caatacagca gtgtaagtag
    14461 ctaaaatata gacatgctca gttggcaatt ctttgatttt ttcgattact tctggtaatt
    14521 cttttgtttc tgtcaattta ttttctggaa tcccggcaac ttttagacgt aaagtcatat
    14581 ctgtatgacg gtctcctcca gaaataacag ctgggatatc catatcggca aagttttcaa
    14641 aattaccatc ccaaatccag ctgacatcga ttccgtctgc gtaattggca tttaataaag
    14701 ccactaaaga aaatggataa ggagctagac caatcatatc gattacttga ttgataccta
    14761 caggattttt gacaagtact aatgtacatt ttttcccatt gatattgatg acttcttgtc
    14821 ggccgaatac tttctcgtca tatcctaaac cttgtttgat tttatcagga tcgacattga
    14881 agtactctgc cactgcagta gctgctaaag cattgtagac attatacata ccgccaactt
    14941 cgattccgta ttcatgacca tcgatcacga aatctgctga cacattgtcc atccgtacca
    15001 tatcagttaa tcgataatcc aattcaggac gtttgaagtc acaatttgga cagtaatatt
    15061 tccctaagtt tgcataagtg atcattttat aatgcaagat gtgatgacaa tttggacata
    15121 agacaccgtc tgtattgtaa tgagccattt gttcacgatc ttcttcatgg tcaaagccat
    15181 aatattttct aggattgact gtttcgatag aattgaagat tggtgaatca ccgttactga
    15241 taatcgttgc atttggtgac tttgcggcac cgtctacaat cattttatag gtcgtataga
    15301 tttctccata ccgatccatt tgatcacgga aaatatttgt aaataagaac agttctggtt
    15361 tcatatattc tgtgactttg ctcaaacttg cttcatctat ttctaggaca gcaaactttt
    15421 tctgtccttt ttttggtttt gcctgcaaga aagtcgatac aattccctgt accatattcg
    15481 caccagtagg gttggttaga acttcatcga attgctggcg caaaatattg actgttaaag
    15541 cggtagtcaa tgttttaccg tttgttccgg tgacaacgac gacttggtag tcttttgcca
    15601 aactatctag tatatgtgga tcgactttca gcgctagttt acctggtaaa cttgatccgc
    15661 ctttaaaaaa agtttttaat gcccattgcg acgtttttcc aactgcaatc gcaaaatgac
    15721 tgcgtattcc cattatgtaa cctccaaaaa cgatttgctg acgaataaat tcaactaatc
    15781 ataccatatg ttgaaaaaaa ggttaaattg cttttcttat tcttaagaaa aggttacggc
    15841 tgaaagtgca tgaaatcgga caaaatcgca tgatttaaca aaaaaacagc ttgaaacaaa
    15901 atgattgttt caagctgctg tgattttatt attccctctt acctatattc ccattttaac
    15961 cgataataat tttctcgctt ggatattcat agcctagatc tcgtgtttct tttggcacga
    16021 ataacatcaa tgtatatagc attccgatcc gcccaataaa catcagtgta gcaatcgtga
    16081 ttttgcccac aacagacaag tctccggtaa tccctaaaga taaaccagtt gttccaaacg
    16141 cagaagttac ttcgatgatg atcgagatca gcgtttgttc ttctgtagca gataaaaaaa
    16201 cgatacaaaa gaaacacata ccaagagaaa gcataaagac aacgactgat tttcgcacat
    16261 catcttgatc gattctccgt ccaaaaatat tgatgttatc ttcacttttg aggaaggaat
    16321 atagatacaa accgatgatt gctacagtag ttgttcgaat cccgccgcct acagaactag
    16381 gactgcatcc gataaacatc agtaaagaaa aaatgatcag tgttgtgatt tgaaaatcac
    16441 ccaaatcgtg aatctgcaga ccagcattac gagtcgtaat cgaataaaac atcgaattga
    16501 tccatttgac actcatattg gaatcttgga ataaatggtc cttttccaat agataaatca
    16561 agacagttcc cgaaacaaac aagataacaa aagctaacac agctaatttg gtaaacaagg
    16621 aaaaacgaaa cggaagcttc gcattggaac gtttgtataa tagccattcc cggcactcca
    16681 tcaatacagg aaaaccaatt cctccgacaa aaatcaaaaa catgatcagg atcaagaaaa
    16741 aatagtcatg tgcaaagggc ttgatggaat ccccagtgac gtcaaaccca gagttcgtga
    16801 cagccgatat cgcctgataa aaaccgtaaa aaacggcatc acgccaacga tcaaaatagc
    16861 ctctgtaata aaaataaata gaaaagaatg tcccaaaaag taattgaaac cagattaaaa
    16921 tagcaaaagt gatccgaatc agtcggacga taccagacaa acgcggttga ttcatatccg
    16981 tcatgatcag ctgtcgctgt ttcagtgtga tccgtcgttt agaaaaaatg acaaaagcag
    17041 tagaaatcat catgatcccc aaaccaccaa cttgaaataa tatttccagc agtataatac
    17101 cgttatcatt gaacacagaa ttgatatcaa aagtcgatag tccagtcaca ctcactgtac
    17161 tgattgccat gaacagcatg tccacaaaag aaacatggct atctggttcg cgaaaaatcg
    17221 gcatataaaa tagaacgagt gataagaccg tcatcaatat ataataaaaa acgatgattt
    17281 ggatcgatga gaaatgattg gcagcaaact tctgtcctcg ccgctgcagc cttcgaaaga
    17341 ataaatcaac atgcacagta ctatcctcct gacaatagtc gtttctacca gtatacaagg
    17401 ggaattagga atactcaaag aaattatcaa acagataaca atacagcttt cgattaaaat
    17461 agaaatggct tataataagg aaaatcctag aaaaaagaag gaggaggaat aacaaattga
    17521 ttaatgctca acaaatcgta gaaaaaatga aaaatcaaaa aataaattat gatacagttc
    17581 tgaaaaaaat gatcatgcaa tgggagaaag aagaagtaaa gcctaagata ttaatacata
    17641 catgctgtgc tccttgcagt acgcacacat tggaattcat gtctcaatat gcagagatca
    17701 cgttattttt ttcaaattcg aatattcatc ctaaaagtga gtatttgcga agattacatg
    17761 aacaaaaacg atttgtagac caatttaatg agaaaactga ccatcatgtt gcgctaatcg
    17821 ttgatgaata ccgtccaaat gaattcacca aaatggtttt aacaaaccat cttgaaaacg
    17881 aaaaagaagg tggtgctcgc tgcagtgcat gcttcaatat gcgattagat cttgttgcgc
    17941 aaaaagcgca agaactagga tacgattact ttggaagtgc cctgaccatc tcgccaaaga
    18001 aaaatgcagc tttgatcaat cagatcggaa tggatatcca aaagatttac gggacacaat
    18061 acttgccaag tgatttcaag aaaaacaaag gctatgaacg ctctttgcaa atgtgcaaag
    18121 agtatgatat ttatcgccaa tgttattgtg gttgcgtgtt cgctgccaag cagcaaggat
    18181 gcgatctgaa agaaatcaat cgggaagcaa aagcttactt aaaaacatca accgtagttt
    18241 ttgaggaaaa ttaaagaaat ctttcggaaa aacgtaagaa aaaaataaag aatttatggt
    18301 agaaataagg tagactgtat aatttgtccg tagtaaagta aggaaacaag aataaatttc
    18361 aatgaaggaa gagataagat gcgaaaagta gcacctattt tgaagccaat cgctgtatta
    18421 acaatctcga cgattgtcgc gactactaca ggtattgtga atagttccag ctaccgtgca
    18481 ccaagttcaa gttctagtac tgtagaagaa acaacaccaa ctacttcagt ctcgacatct
    18541 gaaccaacaa caacggaaag tttgaatgaa accactacga caagtacatg ggacgataca
    18601 acaactagtt cgtcgtatga tgagacatat acaactgaca gctcaagcga aacaagtaat
    18661 acggtaccag atggcactcc atcggattca accaatccaa gtacaagtcc ggatacaagt
    18721 gctagctccg aaacgatacc aagcagtacg actgtagatg ataacacgca aaatgatgat
    18781 gcagcacgta cagcacagca aaaccaagat tcaggaaata cccaaacgta taaccagcaa
    18841 gcaggtgatt ctcaatgaca tttttactag accttatcaa cggggtgcat aaattccttg
    18901 gctatttaga catcagtcct aaatatctta accgtggata tacgatttta agtgtgattc
    18961 ccacgcttta tttattgaga attgtttatg gactttggca aaaccaaaac tatcttcaat
    19021 ttttcttata tggacttgca tttctagtgc ttgtttattt tactgtttta aatgtatttt
    19081 attacttttt agataaaaac acgaaagccg atgtcactca gctttttgtg aagtatttac
    19141 ctgatgaagc gttcaacatc cagacagaag aatcgataga taatggaaat tcgatcgata
    19201 aagtgaatac acaagaggtc atggtttctt atgacgaaga ttatcaattg aaattagccg
    19261 agaatatgcg gtatttgatt ggaaatggtg agatcaaaac aaatgaactc ggtcgcatgg
    19321 atggatttct ggtcgataga aatacattgt atccttatta ctatgtcaag aaaacaggag
    19381 agaaagagta tgttctaaat attggacgaa gctactcgga catggaaaaa gtagggacca
    19441 ttcgacttga ctcaacagaa gacacattgg aacctgtcgg attgttcatc gtcggcggtg
    19501 attttgtcaa agacgggtta cgctatcatg agccttatcg tctgaaattg attgcaaaaa
    19561 aggcacagtc gcaggaagaa ccagtggttt atagtcgtag ccaaagaaga agacattcat
    19621 aatgtgatga aacgccgtgt atcagtggtt gcgataaaaa cgtttattcg gaataagggg
    19681 gatgtgacac gtcttttgtc acgttccttt tttatataac gagaaagata agacgaacac
    19741 atcacttatt ctttcctttc atttataaac attgtagaat aaagaaagta caacgaagta
    19801 ttgtgaaaaa acgtaataaa taacgaggaa aatacaagta ggaggtaagc tgtgaaagaa
    19861 tttttgaaaa attgggggat tttgatcctt gtattagcgg ctattttatt agctagagtg
    19921 tacgtgttta caccagttac tgtaaacggg cattcgatgg accctacatt aagtgatgga
    19981 caacgattga tttcatctaa gatctcaaac tatgaacgaa tggatattat tactacaaag
    20041 gaaccggggg atgaagaacg gatgattgtt aaacggatca tcggtatgcc tggagataca
    20101 gtcaagatgg aaaatgacca gttgaccatt aatggcaaaa agtatgatga accttatctg
    20161 gatgagttca aaaaagaatt ttcagaagat aagttacaag gagaatatgc ttatagctca
    20221 ggattccaag cacaagcaga aagttcatcc acctttacga gtgactttga atatactgtg
    20281 ccaaaaggaa agtatcttgt tctaggcgat aaccgcttga tttcaaaaga cagccgaatg
    20341 tttgggttag tcgacaaaga tatgatccaa ggaaaagttg ttttccgtta ttggcctctt
    20401 tcagaaatta aaatcatgtg atgaaaaaat aaaaagaagc tgtgacatgt tttttgtcac
    20461 agcttctttt tccgaaaatt ccttcttatt tcttggagtt gctcactttt tttgcaatct
    20521 cattttaagc tttttggctg atggataaga aaagcgaagc gtttttgttc ataaatagct
    20581 tttccccacg atggaaggta tgactagtag cagagacaaa gcccgttttt tccaatcgat
    20641 tattcaattc ttcttggcta aagccattat ggactttcgg atgactaatt tgttcattct
    20701 tgtcaaagtc tacgatgatc aattgaccgc caggagctaa aatttcataa agttttgtaa
    20761 gaatattctc cgtatcagga atgtgtaaaa gtacaagtga tagaaggatg agattagctt
    20821 ggacagcagg aaattcgaca gaagcatcag cgtggatcgt tcggacattt ttcagatctg
    20881 ctgcttggat cttttcttct gccattttca gcatagtttc tgaagcatcg gcaatgatca
    20941 gttctttgaa tctttccgca agcggtaaac taacaagccc agttccgcct ccgtaatcca
    21001 gcaggacttt ttggtggctg tcgcgcggta gataagtagt tagttcttcg cgaataatct
    21061 tggctaatgc ttgacggtca ggggaatcat aatggttagc gatggaatca aaaatatttt
    21121 ttgtcatcaa atagctcctt acgtaaaact ttgaaatttt aacttattat agcacgtgaa
    21181 aagaaagaaa gcatgctata ctcaattcaa aaagggagaa tctagaaaga atatgccgat
    21241 atcgtttgta aaagatagag aggaaaaagg aaagtgtgtc cgagaaatat tactagactt
    21301 gcctgagtgg tttgggcttc ctgaatcaac ggaaaaatat atcgaggaat caagtaaact
    21361 acctttatgg tgtgaaaaaa gaaaagaaga atatttaggc tttatcactc tttcacagac
    21421 aagtgaagat actgccgaaa tctattctat tgtatgggaa taaaaaaaga ctatcatcac
    21481 caaggattag gaacaaaact ttttaaagaa gcagaaggtt acgcagcaaa acattacaaa
    21541 tatttgcaag tgaagactgt agacgagggg cactactcta tatatgatca aactatttgt
    21601 ttctacgaat cattaggatt ttcaaggttg gaagtttttc caaatttatg ggatgaatgg
    21661 aacccatgtt tagttcttgt caaaaaattg gaacaaaagt gagcaataga tttaatacta
    21721 atctgtgctt gatgaaaatt ctcactgtaa aaaatgctga aaatcaagta taataccata
    21781 ggaacaaact ggaaggtgaa aaagagcatg gcacaactat tttttaagta tggcgcaatg
    21841 aatagcggca agacgatcga gatcctaaaa gtcgcacata attatgaaga acaaaataag
    21901 ccagttgtct taatgacaag cggtattgat acaagagatg gtttaggcgt tgtttcaagc
    21961 cgaatcggac tgaaacgtaa tgcgatgccg atatttgaag aaacagacgt atttgacgta
    22021 attcagaaaa tggaaagcaa accctattgt gtgctgatcg atgaagcgca attcttaaaa
    22081 aagaaacatg tcattgcttt tacgagaatt gtggacgaat tggatattcc ggttatggca
    22141 tttggtttga aaaatgattt tcgaaacgag ttatttgaag gatcaaaata tttattgctt
    22201 tatgcagaca aaatcgaaga aatgaaaaca atctgttggt tctgccacaa aaaagcaatc
    22261 atgaatttac attatattga tggaaaccct gtttatgaag gcgatcaagt gcaaattggc
    22321 ggaaatgaag cttattaccc cgtttgtcga aaacattact ttcacccatc aatgattaca
    22381 gaaggagaat aagcatgtac gatcaattac aagcaattga agaccgttat gaagagcttg
    22441 gagaactttt aagtgaccca gaagtgattt ctgatacaaa gcgttttatg cagctttcaa
    22501 aagaagaagc aaacacgcgc gaaacagtcg aagtttatcg tcgttataaa gaagtagtac
    22561 aaggaatcac cgatacagaa gaactgcttg gtgaaaaact ggatgatgaa atggctgaac
    22621 tagcaaaaga agaattatct gaattaaaaa aagaaaaaga agtcttagaa gaaaaaatca
    22681 agatcttgct tttaccaaaa gatccaaatg acgataaaaa tatcatcatg gaaatccgtg
    22741 gagcagcagg cggagacgaa gcagcattat tcgctggcga tcttttcaat atgtaccaaa
    22801 agtatgctga agcacaaggc tggaaaacag aagtgatgga agctaacgtt accggaatcg
    22861 gtggatataa agaagtgatc atgatgatca ccggagacaa cgtatattcg aaattaaaat
    22921 atgaaagtgg tgcgcaccgc gtccagcgtg ttccttcaac tgaatcgcaa gggcgtatcc
    22981 atacttccac cgcaacagtt gtcgtgatgc cagaagctga agaagtagag atcgatattg
    23041 aagacaaaga tatccgtaca gatatctatc atgcttcagg tgctggcgga cagcacgtca
    23101 ataaaacagc atctgctgta cgtttgactc acttaccaac agggatcgtt gttgctatgc
    23161 aagatgaacg ttctcaaatc aagaaccgtg aaaaagcaat gaaaatcttg cgtgctcgtg
    23221 tgtacgataa gatccagcaa gaagcacaaa gcgagtatga tgccaatcga aaatcagcag
    23281 tcggaactgg ggatcgttca gaacggatcc ggacgtataa tttcccgcaa aatcgtgtga
    23341 ctgatcatcg tatcggatta actatccaaa aattagatca gattttagca gggaaattag
    23401 atgagatcgt tgatgcattg atcatgtatg atcaaacatc aaaactggaa gctatgcaaa
    23461 atgggtaaaa tgacctatcg agaagtcctg tcacgggctt cttctttttt agaggatcat
    23521 gggaaagaag catatagtat ccaattttta tttttggaac gaaaacaatg gaaaaaactt
    23581 gattggctcc ttcatatgaa cgaagagatt tcagaagaag aacagcggtt gatcgaaacc
    23641 gatctgcgac ttctgcttgc agatcatcca cctcagtatt tgctaggata tgcagatttt
    23701 tatgatcatc ggttaaaggt aactgaagca acactgatcc ctcgaccaga aacagaggag
    23761 ttagtcgaat ggtgtttaga cgagacgcct ggtgtaccgt tagaagtgat agatattggg
    23821 acagggactg gagcaattgc tattagtttg aaagcagcaa gaaaaaattg gcatatatct
    23881 gctgtggatc tttcagaaga agcattagaa gtagcgaaag aaaatgctca gaaagaagga
    23941 acgaaaatca gtttttatca tggagataca ttaggaccag taatggatca gcaatttgat
    24001 gtgattattt ctaatccgcc gtacatcagt cggaatgaat gggaactgat ggatgaaagt
    24061 gtccggtcat tcgaacctaa aatggccctt tttgctgaga acgatggatt agctatttat
    24121 gaaaaaatag cagttgaagc accaagcgta ctaaaatcaa atggaaagat ttttttagaa
    24181 atcggatttc gccaaggtga agcagtaaaa aaaatatttc agcaagcatt tccagataaa
    24241 aaagtggcga taaaaaagga tctttttggc aatgaacgaa tgatccaagt ttatggttga
    24301 agttgtggat gaaaaatttt ttatttgttt ttttattata aaaaaacact tatttgacaa
    24361 ggatgtaaac gactttttgg tttatcaaca agtttatcca ctattgacaa atagattatc
    24421 cacaaacagt ggaaaacttg aggataaact cagacaaaat aagaatgtat gaaaaagggg
    24481 ttgtggataa actgtggaaa cgaaacgata tgatgaaaca gaattgcaag aggcagccga
    24541 agcactgaaa gaaggagaac ttgttgcttt tcctaccgaa acagtatacg gattgggcgc
    24601 caacgctctt ttaccaaacg ctgtcaagaa agtgttttct gtaaaaggga gaccgcaaga
    24661 taatccatta atcgtccatg tcgcctcttt tgaacaagta aaagagtatg tggacaactt
    24721 tcatccggca gcagagaaga ttgtgaagaa cttctggcct ggaccattaa ctttgatatt
    24781 taagatcaaa aaagacactt tgccctcagt tgtgacggga ggattatcca cagctgcatt
    24841 tcgtataccg gataacaaaa aaacactaga agtaatcgaa ctttccggtg ttccgcttgt
    24901 cggtccaagt gcaaatacgt cgggaaaacc aagtccaacg actgcagatc atgtatatca
    24961 tgatctacaa ggaaaaatta ccgggattat tgatgatgga gctacgcgaa ttggtgtaga
    25021 atccactgtt cttgatttaa gtgatccaac agccatgcct atgattttac gaccaggtgc
    25081 agtaacgaaa gaacaaatcg aagcagtcat cgaatctcct gttgctattg atcagcatct
    25141 tgtaaaagaa aatgaaacac caaaagcccc tgggatgaaa tacaaacact actcacccga
    25201 tacacgtgtt ctgatggtca gagatggcga ttggtcaact gcagttcaat gggcaaaaaa
    25261 taagaagatc cgtgcaggtg tcatagcaag ccccgaaatt gcggatcaag tgcggacaga
    25321 tactgcagct gtttatatgt ataacgacaa ctcagtagaa gcggcggcta aaggattgtt
    25381 tgcaggtcta agaggattag atgaaccaac acttggatta gatttgattt ttgtacaagt
    25441 gtatccagaa actggattgg ggaatgctta catgaatcgc ttgaaaaaag cagcaggaca
    25501 aaattatttt gaaaaatgat caattggtta ttgtcgcatt cttttagtat gatacaatgc
    25561 tatcaagtaa atcttctatc agtgaacgaa aaactaagcg taactaatcg taatagaaga
    25621 gatacgaata tttggcaatt tgtttttgat gggaaaaata aaattgtagg aaaataaaat
    25681 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    25741 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    25801 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    25861 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    25921 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    25981 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    26041 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    26101 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    26161 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    26221 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    26281 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    26341 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    26401 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    26461 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    26521 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    26581 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    26641 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    26701 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    26761 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    26821 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    26881 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    26941 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    27001 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    27061 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    27121 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    27181 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    27241 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    27301 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    27361 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    27421 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    27481 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    27541 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    27601 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    27661 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    27721 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    27781 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    27841 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    27901 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    27961 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    28021 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc
    28081 ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt
    28141 tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc
    28201 aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg
    28261 gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat
    28321 cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa
    28381 aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg
    28441 ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag
    28501 atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac
    28561 gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg
    28621 atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg
    28681 agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag
    28741 atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt
    28801 tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt
    28861 ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata
    28921 aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag
    28981 ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca
    29041 atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg
    29101 tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg
    29161 atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat
    29221 tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac
    29281 aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct cagttggtag
    29341 agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag
    29401 aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa
    29461 ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata
    29521 aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga
    29581 tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa
    29641 ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc
    29701 caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag
    29761 cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata
    29821 ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt
    29881 gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat
    29941 ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa
    30001 gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa
    30061 atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt
    30121 ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga
    30181 tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat atttgtattg
    30241 ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta aaaattttct
    30301 agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt ccgagcgtac
    30361 ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag tatcaaaaaa
    30421 gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa
    30481 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
    30541 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
    30601 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
    30661 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
    30721 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
    30781 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
    30841 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
    30901 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
    30961 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
    31021 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
    31081 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
    31141 agtgtattga aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat
    31201 actttgttta tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta
    31261 aaagtgaaga aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga
    31321 tcaaagaaaa ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt
    31381 attatcctaa agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag
    31441 aattgtctaa accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa
    31501 aagaattagg tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag
    31561 ttattcatct caatgaatta aaaagtatag tcaatctcat aatgcaggct aggttcattt
    31621 atttgaacag agtctgtatt tttttattct aaataaagaa acagatgttt ctttatattg
    31681 tattttatcc tttaattaac acaaaaacag ctagtataaa ctaataatgt taattaaaag
    31741 tgtattttat gaaactatcg ttatttatta atgttgcatt tttgtaagtt aaaaattgga
    31801 aatatagatt gaaaaaatga aagcaataag taaaataaaa tgaatatatg cgaaaaaata
    31861 atttacatta ggaaaaacct tgtaaaaaca gttttttggc atatttattt tttataaata
    31921 tttgtaaaat aactatcaat tttttagtta aaagattgga aatgagtggg ggaggaattg
    31981 aagaaaggat ttttgagaga tgatatagta ggttttattt ttagttaaaa taggggagat
    32041 agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta ttactaatat
    32101 aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca gcagtaatgt
    32161 cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa aagtaaaatt
    32221 ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga tcaattggcc
    32281 aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa
    32341 taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa
    32401 tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg ccagaaacgt
    32461 aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg ataaaacagc
    32521 tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt attataaaac
    32581 aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata ttaacaataa
    32641 cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg gacaaagatt
    32701 agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg tttatagagg
    32761 agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta atttttcagt
    32821 aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta ggaaaataat
    32881 ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga atcattcgga
    32941 agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca atcatacagt
    33001 taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat tgaagatttt
    33061 caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat taagacgagc
    33121 agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg aacagggaga
    33181 agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag aagcacccaa
    33241 ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa acgaaaatga
    33301 tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca tcacagcaac
    33361 taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat tattcagagc
    33421 aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag acaatggaat
    33481 aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg aatatacgtt
    33541 taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat
    33601 tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct caggacaaaa
    33661 aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca cagtaaatct
    33721 attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg attggcgcta
    33781 tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata ctgtaacgga
    33841 agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa acagttatac
    33901 accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga aaaattttcc
    33961 agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc aaatatttgc
    34021 tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag ctgtacctaa
    34081 acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa aatctagttt
    34141 tgctgtaatc attggtttat tgatagtaac tatctgtgct gggatattct tacaaaaata
    34201 taaaaaagtt tagttagaat ataatatttt ttgttaaata tgactgaaat caaaagaata
    34261 aaattccgat ttataatcga aaaaagaagc atttttatat atgcttcttt tttcgtagaa
    34321 tcaataaata tggatttggc taactatgga agaatactcg tattagtttt cagcgttcat
    34381 actagttgtt acattgtttg attggccaaa agaatttctg atatattttg tgctaaataa
    34441 agaaaaagac gcttgaattg ggaaataaga aaactactct atcattacaa attttcataa
    34501 actggcagat gttctaagta aacaagtatc ttttcaaagt cggaaaaatc cataatcaac
    34561 gtagtggtat tgatatttgg atgaaagcct acggtatctt gacgatcaat agcagcatcg
    34621 atcacgactt ggattttttt ctctttgtcg tgcatcaatg ctaatggagt cactgtacct
    34681 gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa caaaagataa gcgcttttct
    34741 tttaattgtt cagctagttg ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga
    34801 tagatttgtt ttccttttct tgatttcaat aatagatttt ttacttgcgg accaggtagg
    34861 gaaaaaggga catttttgac tgacgtaatc gcaggatgat cgactttttg aaaagagata
    34921 cccagttttt ttagtagttc ataagcttct tgttcggttg cacgactcat cgcgcatcac
    34981 cctccttttt ttattagtgt atcataaaaa agagttgaca aatgaaaaat aatcatgtaa
    35041 attacacgta aagttaaaac aaatgacatc gaaaagaaga gtagccgtta ggtgttttta
    35101 gagagtctct ggttggtgga aagaggcagc agttaaccgt gaaacacatc ttggagtcga
    35161 ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag
    35221 atgaccagat aaggctggct tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga
    35281 ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt tcatctgaac ggcaacgact
    35341 gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg gaaccacgta taatacgtcc
    35401 tcttggcatt tgccaagagg atttttttat tt
//
LOCUS       DANKHJ010000024        34962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000024 DANKHJ010000000
VERSION     DANKHJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PW113_001286"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PW113_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2525608.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PW113_001287"
     CDS             complement(2020..2922)
                     /locus_tag="PW113_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2525609.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PW113_001288"
     CDS             complement(2919..3611)
                     /locus_tag="PW113_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2525610.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PW113_001289"
     CDS             complement(3654..4295)
                     /locus_tag="PW113_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2525611.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PW113_001290"
     CDS             complement(4333..4647)
                     /locus_tag="PW113_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525612.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PW113_001291"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PW113_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PW113_001292"
     CDS             complement(5984..6916)
                     /locus_tag="PW113_001292"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2525613.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PW113_001293"
     CDS             complement(6913..7689)
                     /locus_tag="PW113_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2525614.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PW113_001294"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PW113_001294"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2525615.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PW113_001295"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PW113_001295"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2525616.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PW113_001296"
     CDS             complement(12014..13294)
                     /locus_tag="PW113_001296"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2525617.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PW113_001297"
     CDS             complement(13313..14239)
                     /locus_tag="PW113_001297"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2525618.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PW113_001298"
     CDS             complement(14252..15535)
                     /locus_tag="PW113_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2525619.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PW113_001299"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PW113_001299"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2525620.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PW113_001300"
     CDS             complement(16323..17234)
                     /locus_tag="PW113_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2525621.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PW113_001301"
     CDS             complement(17231..17695)
                     /locus_tag="PW113_001301"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2525622.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PW113_001302"
     CDS             complement(17700..18398)
                     /locus_tag="PW113_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2525623.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PW113_001303"
     CDS             complement(18385..18771)
                     /locus_tag="PW113_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525624.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PW113_001304"
     CDS             complement(18918..20588)
                     /locus_tag="PW113_001304"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2525625.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PW113_001305"
     CDS             20771..21004
                     /locus_tag="PW113_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2525626.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PW113_001306"
     CDS             complement(21103..21534)
                     /locus_tag="PW113_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2525627.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="PW113_001307"
     CDS             21613..22026
                     /locus_tag="PW113_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2525628.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="PW113_001308"
     CDS             complement(22064..23212)
                     /locus_tag="PW113_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2525629.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="PW113_001309"
     CDS             complement(23334..23930)
                     /locus_tag="PW113_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2525630.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PW113_001310"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PW113_001310"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2525631.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="PW113_001311"
     CDS             27160..27591
                     /locus_tag="PW113_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2525632.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="PW113_001312"
     CDS             complement(27636..28985)
                     /locus_tag="PW113_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2525633.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="PW113_001313"
     CDS             complement(29055..30218)
                     /locus_tag="PW113_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2525634.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="PW113_001314"
     CDS             30347..30670
                     /locus_tag="PW113_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525635.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="PW113_001315"
     CDS             complement(30853..32202)
                     /locus_tag="PW113_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2525636.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="PW113_001316"
     CDS             complement(32271..33224)
                     /locus_tag="PW113_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2525637.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="PW113_001317"
     CDS             complement(33326..34516)
                     /locus_tag="PW113_001317"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2525638.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DANKHJ010000025        33265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000025 DANKHJ010000000
VERSION     DANKHJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(41..229)
                     /locus_tag="PW113_001318"
     CDS             complement(41..229)
                     /locus_tag="PW113_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2525639.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="PW113_001319"
     CDS             499..861
                     /locus_tag="PW113_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2525640.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="PW113_001320"
     CDS             913..2595
                     /locus_tag="PW113_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2525641.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="PW113_001321"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="PW113_001321"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2525642.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="PW113_001322"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="PW113_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2525643.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="PW113_001323"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="PW113_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2525644.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="PW113_001324"
     CDS             6430..7434
                     /locus_tag="PW113_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525645.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="PW113_001325"
     CDS             7435..8379
                     /locus_tag="PW113_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525646.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="PW113_001326"
     CDS             8379..9341
                     /locus_tag="PW113_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525647.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="PW113_001327"
     CDS             9368..10282
                     /locus_tag="PW113_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525648.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="PW113_001328"
     CDS             10308..12089
                     /locus_tag="PW113_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2525649.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="PW113_001329"
     CDS             12437..13123
                     /locus_tag="PW113_001329"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2525650.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="PW113_001330"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="PW113_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2525651.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="PW113_001331"
     CDS             16733..17542
                     /locus_tag="PW113_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2525652.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="PW113_001332"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="PW113_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2525653.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="PW113_001333"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="PW113_001333"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2525654.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="PW113_001334"
     CDS             complement(21094..21546)
                     /locus_tag="PW113_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2525655.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="PW113_001335"
     CDS             21696..22646
                     /locus_tag="PW113_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2525656.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="PW113_001336"
     CDS             22639..23598
                     /locus_tag="PW113_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2525657.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="PW113_001337"
     CDS             23595..24350
                     /locus_tag="PW113_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2525658.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="PW113_001338"
     CDS             24373..25326
                     /locus_tag="PW113_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2525659.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="PW113_001339"
     CDS             complement(25970..26266)
                     /locus_tag="PW113_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2525660.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="PW113_001340"
     CDS             26621..26977
                     /locus_tag="PW113_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2525661.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="PW113_001341"
     CDS             26987..27886
                     /locus_tag="PW113_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL2525662.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="PW113_001342"
     CDS             28403..29134
                     /locus_tag="PW113_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2525663.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="PW113_001343"
     CDS             29279..31306
                     /locus_tag="PW113_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2525664.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="PW113_001344"
     CDS             31414..32511
                     /locus_tag="PW113_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2525665.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33265
                     /locus_tag="PW113_001345"
     CDS             32818..>33265
                     /locus_tag="PW113_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525666.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        11138 a         5762 c         7125 g         9240 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaaggatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gttga
//
LOCUS       DANKHJ010000026        31348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000026 DANKHJ010000000
VERSION     DANKHJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..170
                     /locus_tag="PW113_001346"
     CDS             <1..170
                     /locus_tag="PW113_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2525667.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="PW113_001347"
     CDS             complement(553..1281)
                     /locus_tag="PW113_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL2525668.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="PW113_001348"
     CDS             complement(1259..2833)
                     /locus_tag="PW113_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2525669.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="PW113_001349"
     CDS             3062..4000
                     /locus_tag="PW113_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525670.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="PW113_001350"
     CDS             4012..4923
                     /locus_tag="PW113_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525671.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="PW113_001351"
     CDS             4947..6419
                     /locus_tag="PW113_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2525672.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="PW113_001352"
     CDS             6470..7081
                     /locus_tag="PW113_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2525673.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="PW113_001353"
     CDS             7111..9024
                     /locus_tag="PW113_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL2525674.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="PW113_001354"
     CDS             9152..12133
                     /locus_tag="PW113_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL2525675.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="PW113_001355"
     CDS             12130..13791
                     /locus_tag="PW113_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL2525676.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="PW113_001356"
     CDS             13831..14763
                     /locus_tag="PW113_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL2525677.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(15155..16702)
                     /locus_tag="PW113_001357"
     CDS             complement(15155..16702)
                     /locus_tag="PW113_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2525678.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16804..17157)
                     /gene="tnpB"
                     /locus_tag="PW113_001358"
     CDS             complement(16804..17157)
                     /gene="tnpB"
                     /locus_tag="PW113_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2525679.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(17147..17341)
                     /locus_tag="PW113_001359"
     CDS             complement(17147..17341)
                     /locus_tag="PW113_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525680.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17567..18769)
                     /locus_tag="PW113_001360"
     CDS             complement(17567..18769)
                     /locus_tag="PW113_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2525681.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18772..18966)
                     /locus_tag="PW113_001361"
     CDS             complement(18772..18966)
                     /locus_tag="PW113_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2525682.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(19013..19861)
                     /locus_tag="PW113_001362"
     CDS             complement(19013..19861)
                     /locus_tag="PW113_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL2525683.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(20066..20416)
                     /locus_tag="PW113_001363"
     CDS             complement(20066..20416)
                     /locus_tag="PW113_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525684.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            21011..21457
                     /locus_tag="PW113_001364"
     CDS             21011..21457
                     /locus_tag="PW113_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2525685.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            22081..23073
                     /locus_tag="PW113_001365"
     CDS             22081..23073
                     /locus_tag="PW113_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525686.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            23089..23973
                     /locus_tag="PW113_001366"
     CDS             23089..23973
                     /locus_tag="PW113_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525687.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            24022..25629
                     /locus_tag="PW113_001367"
     CDS             24022..25629
                     /locus_tag="PW113_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2525688.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25630..27114
                     /locus_tag="PW113_001368"
     CDS             25630..27114
                     /locus_tag="PW113_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2525689.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27291..28283
                     /locus_tag="PW113_001369"
     CDS             27291..28283
                     /locus_tag="PW113_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525690.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28335..29204
                     /locus_tag="PW113_001370"
     CDS             28335..29204
                     /locus_tag="PW113_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2525691.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29624..31189)
                     /gene="guaA"
                     /locus_tag="PW113_001371"
     CDS             complement(29624..31189)
                     /gene="guaA"
                     /locus_tag="PW113_001371"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2525692.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(31244..>31348)
                     /locus_tag="PW113_001372"
     CDS             complement(31244..>31348)
                     /locus_tag="PW113_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2525693.1"
                     /translation="NNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         9822 a         5236 c         6325 g         9965 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gttgaaaagt aagcgcccca tagaacagta cctgtacgtc aaataacccg
    15121 acctatagaa tagaatcggg aatgtttggc gtttttagtt acgagaaaaa tgttgttgta
    15181 catccttgtt ccaaggcaac agttgatcta aagtttctgc tgaaagtacg ggaagatttg
    15241 gtatcttatc aaatagaaac tctagatact ttacaggatt caatccgtta agttctgctg
    15301 ttttatatac actcagaatg attcctgaag ttcgtgctcc ttcaagactt gtggaatgca
    15361 accaattttt ccgtcctatc accagctctt tgatatttcg ctccgcacga ttatttgtga
    15421 gttctaaacg accatcttca aaaataggtt caaaaccact tcgtagttta cacgcatatt
    15481 gaacggctgc atccaacttg gattttgcaa caggatctat cgttgccatc cactcataaa
    15541 aagcaataaa taatggacgc agttcttgtt gccgacagct taatcgcttc tcaggagaaa
    15601 agtctttcca tttcttttct aggacaaacc atttatccaa ttgtttgact gcttgagctg
    15661 cgggaatatc cgtgtttttc tttccttttg gaattgcttc gaaaaatttc cgccgaacat
    15721 gcgcgaaaca atagacaagt cgtacacttt ccacggcatt atacccgcta tagccgtcac
    15781 agtgtaaata ccctgtaaac tccttgagaa aagttttcgg aacatctcca gcacgacttt
    15841 cgtcgtggtg ataataaacg atgggatgct gactgtcttt tcctgtacaa aactgccagt
    15901 aataggtatc tgttttggca ctttcaatca cacgataact tgtttcatcc ccgtgtagaa
    15961 tctcttcttg attaagatag actcttaatc gttctcctaa agggtctaac gcgttttgca
    16021 ctgctttaat atgccaattg gtgatattat ctctcgaaat atccaaccca tgtgtttgtt
    16081 tccaatagtt ctcttgccga taggcaggaa ctttctgacc aaatttcatc cggatcgttt
    16141 ccgccactaa actattggaa cctaagctat tagaaatcaa aggtttagga acgatcgctt
    16201 ttttaaaaga agaaagtcct tctttttcgc agcttttaca acaataggta tgttgataat
    16261 gggtgagaca atccaacata gcctgatgga aacgaatttc ttctcgtact tttgtccgtc
    16321 cgatatcttt catctgtgaa ccgcaatgtt cacaaaattg ttcttccaaa ggaaggagat
    16381 gttctttctc tgttataggt aagtgtttga ttttgtctgc tttcgttcct acaactcgtt
    16441 gatgtccttt tactggaata atggtggcag ctgcttctgg aacacttaca gaaatttctt
    16501 catcactaaa aagattcaat tgcccattcg tttcctcaaa gagagtctct ttagagcgtc
    16561 caaataattt gttggttaaa taattgacct tttcattcaa tagttggatt tcagttgtaa
    16621 gattctgaat ggtttgttgt tgtgcttgat tggttttatg ggcttcttga agcaactgaa
    16681 gcaacgtatc tgtagtttcc aaaatttttc accaccttga atcgaactga ttttgttaca
    16741 gaataccaca agaaacgcac aagaaatagg ggttcaacac attttttgtg ttaacccccc
    16801 agcttaaaaa actccttttt ttcctggcaa aatagctcga ggttgatcga tacttaatcc
    16861 ttctaacaac catcgaacct gttggttcgt taaggttcga acttcgtctt tcgtccgagg
    16921 ccattttaac ttgccgtttt ctatccgctt ataacagaga agaaaaccat caccgtccca
    16981 atagagaagt ttgaaacggt cttgacggtt cccgcaaaat aaaaaaacgg catcttcgta
    17041 aagctcgagt tgatactctt gttgaataag actggcaaga ccatcaattc cttttcgcaa
    17101 atctgttttt ccgcacacaa tataaatatg tttggtttga agtaaatcat aaagcattgg
    17161 acaacagttc tttcaaaata gcttgaagaa tataattgtt aataccgtta tagatcaaaa
    17221 cttcagaacc attctctctt ttgatgcgca tcgctaactt ggcagccgcg ggtttacctt
    17281 tttctggtgc actcttttcg tttcctaatt ggacaggaac aatctcattt aatttataca
    17341 tagaaaacct ccagacttct tgatagctca agtatatctt gaggtgcatt tgttagtaag
    17401 gtactgttct atggggcgct tacgttgaaa aacgttgatt tatcggcatt ccccatccat
    17461 attatattta cgtcttattc ccactcaata tgagtagcct cgtaaagaag ctattatatc
    17521 agtgcttttt cagcctattc ctcattttgg tagggaaatg gtagggttag atttttactg
    17581 cttgatttcc attaaagttt gcaactttag attttgttgc acaggtaaat ttaatgtttc
    17641 ctgtcatttg ttctgcaatt ccttcatcat tacggctcca tagatgagcg tagtgcttta
    17701 aagtgatttc agggctagaa tggcctaacc gttgcgacac gaccagaatg tctgcgttaa
    17761 actcattgat taggtagcta acatgagaat gacgtaatcc ttttccttgt attaggggta
    17821 ccttagctag tttggcataa cgctgaataa ttcgttgaac agttgaacga tagagtggga
    17881 ggtcagtgta gctaagaata aattgttcta caccatgatt tttttgaata actttccact
    17941 tttttagcac ttcaattgta tcatcgtcca gagaaatgat ccgcaagccg ctttcagttt
    18001 ttgtgtaagg tttccgaata aaatcttttt gatttgtcat ttccaaggtg tgatgaaccc
    18061 tcaacttttt acgtttgaag tccacgtcat tccaattaag tgctaatcct tcgcttacac
    18121 gaacaccagt catgtaatat agccagatca ttaaaaaaca catatgttcg tagaaatcat
    18181 ctttataaat ttttgccaaa acttcttcaa attcttcttt tgtccagtaa ggaacgattg
    18241 attttccttt tggaattgct tttgttcttt tggaaatatt ctcattcaga aattgcaggg
    18301 tgaccgcata gtctaatgat ttacgaaaca taccgtacat aagtgaactg tatgcttgtg
    18361 agtatccgct atcgttcaat aaccaaattc gataattttc acaatctcgc acattgattt
    18421 cacgaagctt cttatcaccg aaaaattcag taatctgaat caatccacac tcacgagaac
    18481 tccaagtact tttttgtacg ctagctttgt agtgtggaac gtaagactgc tccataaatt
    18541 ctttgtaagt taaatcataa ttcgcaaatc catttacaac catgtattca ttttttatac
    18601 gcatgacttc tttgtgtgct tcacgagctg atgagaatcg ttttcctaga gaatcttttg
    18661 ttcctttctt ctggatcctc tttccagtca cttgatctgt tcctaactca actagataag
    18721 aaaagcgacc atttttatct tgatacacac aagggtattt tgttttcata gttattcctc
    18781 ctgtgaaaga ggaacaccta taatttcttc aattacttgt cggggtacta ccccaactct
    18841 tttattatta taatatggat atccctgatt taccattctt gtttttgctt gtcggattat
    18901 tgtggtagct tgatgtgttt taaatcctag agcctctaaa tcttttcgac aaattgtatt
    18961 tttcattgta taaactcact ttcaaagaaa acactatcat tgggtaatag tgttatttct
    19021 ttatttttgt ataatgaacg gtactttctt gaaaatccat cacttgttca ggacttccca
    19081 actcatttaa agctaaagca ttgtaaaaaa atggacgtct aactatatat tcgcttgtat
    19141 atagtatatc ttgattcgtg gaatcatagg gtttctttac catctttttg gtgacagttg
    19201 gtagtttgag attccgggac ttgaaaaaag cttttttctt atgctctttc aattccagct
    19261 ctttatcgaa atatttgctg atatagcgcc cgcgattttc gatactgtct acatcgatct
    19321 tgttgatctt aacgaaaccc tgaccccaaa tcgtagttaa ccggttaaat gagataaagg
    19381 ggaaatcaaa caagataata tggtagtgga ttgcaccgcg ttgctgcttt tcccacgtgg
    19441 cgagatactt taaatttgct tttttagttt tatagaggtc ataattcagt cgtttgataa
    19501 attttttaaa ttcattattt gcatactcaa tgtcttgaat gttttctttg aaagtgagag
    19561 ttaaaaattt cgtttgattg tcaaaattcg tgtctacaag gcgggcaatc tcaaatcgca
    19621 tactttgata atgtttctgt ttgcgtttta aactgtcata ttgtccttga gcggataatt
    19681 catcatagtt ttttcttttt tcaagagtaa tatcattttc ataaatccaa gctaattttt
    19741 gttttcgttc cagatttact tcgttttcga tcgttttacg tttgggagta atgattgctt
    19801 cataatcata aatttcaata tacgtgggag tttcaatgat tttctgatta taagcttgca
    19861 aaaatcatca ctccttgttg cgtattcgat tgtcaaagaa ccagtcattt tctcctgatg
    19921 ttgatctata aatcaagtta agggggaagg cctgcgtagc agcccttcta gcctatgggg
    19981 tgttgggcgg tcttaacgga cagaaacggc tctgtgggcg ctgccccgcc gtttcgtcct
    20041 aaccacccaa cactccatag tatagttagt tttgtgtagt ggtaccttgt tttctgggaa
    20101 tcaaagttgc cgtatcagcg gttgccgata acccataact gacaaattgt ccattcgagc
    20161 gtccccaagg tacaatcacc aaattggtaa atgtaattat ggctaaacct tgttgggtaa
    20221 gttcttccgg cgtgatcaca ggatgttcag atttgacttt gacactgatt ttttcgtagc
    20281 gaagttttgg taggatacat tcgtagctcc aacctagaat ttcgccctcc ttttgccagc
    20341 gagtgacatt gacgacttgg tattcttttt cgagtgtttc ttttggtaaa ctgagatcgg
    20401 taatttttat tggcatcaca attctccttt tttatacgaa agtttatacg atttgattaa
    20461 aaaatataga tggccatcta cgacttgtat tatatacgag aacttatacg tagtcaagag
    20521 attttctaaa aaaatatata cgatttttta tacgagtgtt ataatgaaaa aaatgataag
    20581 gaggaatttg aatatgtttg gtttagtaca acctaataaa gtgaaggtgg gccaacgaat
    20641 caaagaaata aaagacggaa tgtctctctc ttttacagaa ttagggaatc gattaggttt
    20701 aaaaaagccg acgatcagtt cctacgttca aggctatgcg ttggctccta agagtgtgat
    20761 caaccaatta tctagtatca gcggaaaacc tgtcggctgg ttttattttg ggcatgtgga
    20821 agaatatatc cgagagtatt tgtttctcat tggtcagaaa aaaatagtgg acgattatcc
    20881 tgaaatcatt gagaaaataa agcaagaatt ttatacgggc gagttcaaaa atcctgcttg
    20941 ggagaatgag ctaggctatc ctttggagga atttatccaa gaatgttttg tagaatattc
    21001 gagtgaaatc atgaaggact atgtcgtaca gattacgaaa gaggtacttg aagaatccaa
    21061 aacgctaaac aaactatcat caaaagcaaa agaagaagct gtcacgattc tttcatctgg
    21121 gatcattgac tatatcgaga tgagtggtga gatcaagtat ggagagaaag atcgcattgc
    21181 gaaaatggtg aaagaatcaa taaaaacctt tgatgtgaaa caagacctga catttaatga
    21241 ttcctatttg attggaaagt tgatcaatat tcttggtaca gatgaagata cggagctgtt
    21301 tctttcttct ttatcaatga tatttactga taagcatttt tccactcgat ttggtgggga
    21361 agaattagtc gaaatatttc aatcgatgcg accagcgtta atcaaactct atcgggagaa
    21421 aacaccagat gaattttatg attggtttga gaagtaaaat aagaaaagtg gcttagatta
    21481 caagttggtg aaaaaatttt tttcagcata acatcagtaa tctataaagg ggttgttgat
    21541 tatactgtag aagttaagta ctcaagagat tttttgtacg taaacaaagc aaaaaagggt
    21601 gcagtttcac tagaaaatgt actctttttt gtgatttttc ttagttatag agtttttttg
    21661 aaactttaaa aatgagaaaa catattgatt ttaaagagtg tagacatagc ttcttttgct
    21721 aaatagatta aacttactgg tgagtggtct tgaacatact tttaaatcac attttactga
    21781 gttttattgg attttataat tcattggaaa cctcgtactt aatctgtttt tatctatcga
    21841 ctagaatctg tatatattcc agctatcaat gaggccgcag aaaagggtgc ttaaaaataa
    21901 tctgttgttt aaaatattat tatcagaata cttagataca aaattctggc acaaagtaaa
    21961 aaaaatattg acttaaaaaa atggatatgg taatctataa acgaaaatag tggtaacgtt
    22021 tccaaaatgc atcttcgtta aatttttatt tttttaaacg aaaatggaaa cgtttctaaa
    22081 atgtattggg aggagattga attggaaaag acattgggac caggtggtga gataagggcg
    22141 gtacaacggg ataaaaaaaa tatttcgaag tgggaaaaat taaagaaaca gttgcggcgt
    22201 gataagtgga tgtacttgtt gctcttacca ggtatcatca tgacgtttat ctttaagtat
    22261 gtgccaatgt acggtgctgt gattgcattt aaagactata atcctatgct aggaattttg
    22321 gaaagtcctt gggtaggact agatcacttt agggaattta tcagttcacc aaattttacg
    22381 cttttgctga gcaacacatt aaaaattagt atttatggtt tattgttagg tttttttcca
    22441 ccaattattt tagcactgag cttgaatcaa attatcaata aaaaaataaa acagaaaatc
    22501 cagttgattc tatatgcgcc gaattttatc tcagtagtta ttattgtggg gatgctgttt
    22561 ttgttcctat ccgcaaatgg acctattaat gggttagtag aaaaggttac ggggcaacca
    22621 attatgttca tgtctaatcc agcttatttc agaacgattt atattttatc gggaatttgg
    22681 caaggaatcg gctggtcatc cattctttac accgcaacgc tggcgggagt cagtaccgat
    22741 ttatatgagg cagcaaatat cgatggggca aatattttac aaaagatttg gcatatcgat
    22801 ttgcctgcat tgaggcctgt gatggtgatc agcttcatct tggccgccgg cggtattatg
    22861 aatgtaggat atgaaaaagc ctacttgatg caaacttcaa tgaacatccc tgcctcagaa
    22921 atcatttcca cttatgtata caaagtcggc ttgcaatcag gtgattatgc ctactctgct
    22981 gctgttggtc tgttcaatat gattattaat gtcgttttat tactgattgt caacaatata
    23041 accaagcggc taaacgatgg tcaaggcttg taatgaaagg agaaaattat gaatagtgct
    23101 ttatcaaaaa tcgaccgtca aatcttatgg ttgaatcgag tgattgtaac ttttttagtc
    23161 gtcatcacca ttgttccgct gatttatgta ctggtagctt cttttatgga cccagctact
    23221 ttgatcaata aaggtataag cttcaatccg aaagattgga caatacaggg atatcaacgg
    23281 gtatttgcag atgattccat tttacgcggt tttgtaaatt cgttgtttta ctcttttagc
    23341 ttcagcttat tgactgtatt tattacgatg atcactgcgt atcctttgtc tcgacctaac
    23401 ttggcgggga aaaagattat tatgactttt tttgtcatta caatgttcgt gggtggtggc
    23461 ttgataccta cctatttatt ggtaaagaac ttgggcatgt tagatagtgt atgggcgatt
    23521 atcgtaccag gtgctataaa tgtgtggaat attatcttag cccgtaccta ttttagtgct
    23581 ttgccaaaag aactttctga agctgctact atcgatggcg ccaatgactt gcaaatactg
    23641 ttcaaagtca tgcttccatt agcgaaacca attatgttcg tattgttttt atatgccttt
    23701 gttgggcaat ggaactcata ctttgatgcc atgatttaca tcaaagatcc cgacttacag
    23761 ccgttgcaac tcgtattaag aaatattttg atccaaaacc aaccttcaca agacatggtg
    23821 ggagccaata ctgccatggc agaaatgaaa caaattgctg agttgatcaa atacgcaacg
    23881 atcgtcattt ctagtctgcc attaatcata atgtatcctt tcttccaaaa atattttgat
    23941 aaaggtgttt tagttggttc tgttaaaggg taagtattgt tcactatttg tatgaataaa
    24001 aattataaag gagattttaa gatgaaagca aaatggatgc tgccaatcgt ttttacttcc
    24061 gtactaattt tgggggcttg tggaggctca aaagaaaaga gtaaatcaag ccctgattat
    24121 gaactagaaa atgtctcttt tccattgaaa gaaaaagtat ctttaaaaat gatgtctcaa
    24181 agtgcccctt tggctcccaa tgatcctaat gacaaattga ttttccaacg attagaagaa
    24241 gagaccaatc tccatattga ttggacaaac tacagtagtg attttgccga aaaaagaaat
    24301 ttggatatcg ctagtggtga tttaccagat gccatcttca acgccgctgc aggtgactat
    24361 gatttattaa actgggcgga aagtggcgtt attgtaccga ttgaagattt gatcgaagat
    24421 tatatgccaa atctgaagaa aatttttgat gaaaaccccg aataccgaca aatgtctact
    24481 gcacctgatg gtcacattta ctctatgcct tggattgaag aattaggcga aggaaaagag
    24541 tcgattcata cagtaaatgg gatggcttgg atcaacaagg agtggttgga taatttgggt
    24601 cttgaaatgc cgcaaaccac tgatcagttg atgactgtgt tggaggcttt caagacgcaa
    24661 gatccaaatg gtaatggtga agcagatgaa attcctttta gctttattaa caatggagga
    24721 aacgaagact taaaattttt aatgggggca ttcggtctag gtgataatga tgaccactta
    24781 gtcgttgata acgatggtca ggtacaattt actgctaaca aagaagggta taaagaagcc
    24841 atcaaatatt tcaatgaaat gaataataaa ggtcttattg ataaagaggc tttcgaacaa
    24901 gattggaata cgtatgttgc caaagggaaa gaacataagt atggcgtgta ctttacttgg
    24961 gataaagcaa atatcacggg tgcagatgat agttatgacg tcttgccagt cttagcggga
    25021 ccagatggaa cgaaacatgt tactcgtacg aatggcatgg gtttctcaag agatcgtttc
    25081 gtaattacta gtgcgaataa aaacttggaa cttacagcta ggtggataga taaaatgtat
    25141 gaaccgttac agtctgtcca aaataactgg ggaacctacg gtgatgaagc acaggcgaat
    25201 atttttgaat taaaggatgg tatgttaaaa catttgcctt tagaggggac agcaccgggg
    25261 gaactaagac aaaaaaccga agctggagga ccattagcag ttcttgattc ttattacggc
    25321 acagtcacta ctatgccaga tgatgcgaag tggcgtttag atttgatgca tgacacgtat
    25381 ttgccatata ttacagaaga caatatctat ccacgtgtct ttttagaaaa agaagaaaca
    25441 gatcgattag caaaaatcga aacggatatg tgggattaca tctatcgcaa aagagcagag
    25501 tggatcaccg gcggtaaaat cgatgaagaa tgggaagaat accttgctga attggaacgt
    25561 gtaggtttgg aagaatggat tcaaataaaa caaactggct atgataattt tttgaaggga
    25621 gcacaataag tgttagtaga agaaacggcg attttggata aagcaaatcg atttattgaa
    25681 atgatgcgag gaaaagttaa ccctcagtat cgacaaaact atcatttttc cgcacccatc
    25741 gggtggataa atgaccccaa tgggtttata tattacaaag ggcattacca tctcttctat
    25801 caatactatc cttacgatag tcgatggggg cccatgcatt gggggcatgc gcgcagtagt
    25861 gatttggtcc attgggaaga tctaccagta gcgttagccc cagatcaacc ttatgacagc
    25921 ggcgggtgtt tctcaggaag tgcaattgaa agagacggaa aactatattt attttataca
    25981 ggctgtcaag aaattgatgg tattagccat cagcatcaat gtttggcggt ctctgaagat
    26041 ggtattcatt ttagcaaata cagtggtaat cctgtggtag cagaaaaaca gctaggtgaa
    26101 aatgggctaa tacaagaatt tcgtgatcct aaagtaattg ttcacaacga tggttactat
    26161 atggtagtag ctactaaatc tacacagtct actggtagaa tagtgatgtt tcaatcgtca
    26221 gatttgtata cttggcactt ttactctgtg ctgctagaag ggacttcaga gcaaggaata
    26281 atgtgggaat gtcctgattt attccattta gacggacgag atgtcttaat catgtctccg
    26341 atacaaatca agaagaacgg actggaatat cataatatca gttctaccat ggcttgtatt
    26401 ggtgaggtaa actgggaaac aggtcagatg atagttgata atttccatga aatggatttt
    26461 ggaatggatt tttatgcacc acaaacaaca agagatccta atggtggacg ggtgatgatt
    26521 gcttggatgc agatgtggga tcgtacattg cctacacatg agttaggcca tctttgggca
    26581 ggctctatga gtttgccccg agagttaaga gtgaaaaaaa atcgattggt acaaaaaccc
    26641 atctcaactg tatatagtca tttagtttat caacaaggca gtgaaaatat tgcagtgact
    26701 gacacatcag tcgtatttcg aaacgcgctg tctgattctt catatttaca tctagtcgca
    26761 gatttggaga aagctgaagt ttttacaatt gaattactgc ggtctaagac ggattgtctc
    26821 aaactagcct atgacactgc tacacaggtt tttgaattgg atcgtcagaa actaggaaga
    26881 gagcttacag gggcggaaga agaccctctg aatgtacgaa aagttgaagt acctttgatt
    26941 gataatcagc tcgttcttga gatttttaga gatacctcat caattgaagt ctttattaat
    27001 gggttagaag ttatgacggc tactttctat gagctggaaa aagctcgaga tatcttgttt
    27061 cattcgatcg gagaaagtcg tattcaagct tttgaaacag cgagtgtgaa ataaatgttt
    27121 gattgcttag gcgatgaatc aattatttaa taggttttga aggataatgt ttttagaaaa
    27181 gaacagatag ctagatttcc catggatttg ctattagtat tttgaaaaaa ttagtaattt
    27241 atgacaaaat tcattatact aaacttgtgc agaaatgtga gggatcaact atgaaagcaa
    27301 caatgaaaga tgtcgcaaaa ttagcaggtg ttggagtagg aactgtttcc cgggtattga
    27361 ataacggttc tgttaaagct tctactagag aaaaagttga agcagcaatg acagagttaa
    27421 actatcaacc agatttttat gcccgggggt taaagatgaa gcatacgaac acggttgctt
    27481 tgattctacc cacggtatgg catccatttt ttggcgaatt tgcctaccat gtagaaacag
    27541 cattagaaag acaaaactac aagctttatt tgtgtaactc cagtggtaat agtgaaaaag
    27601 aacaagaata tatccagatg gtcagccaaa ataaagtaga cggcatcatt ggaattactt
    27661 attcagatat cgatgaatac atttcgagta atctgccttt tgttagtatt gaccgttatt
    27721 tcactgaaga ggtgatttat gtgaccgctg acaatgcggc cggtggacgt ttggctgcaa
    27781 atgaacttta tcgccgagga tgtcagaatg tagcatatat cggtgggtac caagaaacac
    27841 cgaatgaaac gaagagtcgt cgaaaatatt ttgaaatcga gtgccgtcaa ctggggattt
    27901 cctataaagc gttagatatg ttagaacctt tagaccatct ctcagaacgt gtaacaaatt
    27961 ttttgttaga gaatcccgat atcgatggga tttttacgat caacgatcca atggcattag
    28021 ctgtcattga tatgcttgaa aaaataggga aaaacgtacc agaggatgtc caagttatcg
    28081 gatttgatgg tcaaaagatg acagaaggag atccttatct agtctcaacg attgcccaac
    28141 ctacaaagca aatggcggaa gtggcagtgg caaaattaat tgctgtcatt aatggagaga
    28201 cagtagaaaa acgtaccgta ttgccgattg aatttggcga aggtgacaca acgaaacctt
    28261 tagtgagaaa cgattttgct tagtgcaaat acgtttcttt ttttgaaaat gagtgaatga
    28321 aaggtgtaga taaaatgtta tatggtggaa tagaagccgg tggaacaaag tttgtctgtg
    28381 ggattggtaa tgaacaatta caagtgatag aacggaaaag ttttcctacc acaacaccag
    28441 aagaaacgat ggagcaagtg tttgatttct ttgataagta caaaggaaat atcgcatcaa
    28501 ttggtgtagg agcatttggg cctattgata ttcaaatcga atcaagaact tacggatata
    28561 tcaccaacac accgaagtta gcttggcaaa attatcattt tgtaggagct ttggaaaacg
    28621 ccttgtcggt tcccatctat tggactaccg atgtaaatgc tgcttgttat ggagaatata
    28681 tggcagggta tggaaaaggt aaaaatagtg ttctgtatta caccgttggt acaggtgttg
    28741 gtggtggtgc tgtagtggat gggaaaatag tcgcaggttt tagtcatcct gaaatgggac
    28801 atatgctctt acaacgtcat aatgaagatc aatttgaagg aatctgtcct tttcatcatg
    28861 attgtttgga agggttagca tcaggacctg cgattgaaaa acgctttgga agaaaagccc
    28921 agactatctc atttgatcat ccttattgga cgattgaggc agattatctt gctcagtgtg
    28981 tgtataacac aacgttacta tatgcaccgg aagtaataat tttaggcggg ggtgtaatga
    29041 aacagccgca actactcgag ttgattcgcc aatcgtttaa agaaaaaatg ggttcctacg
    29101 tggatacgcc tactctcgaa gaatatctac taactccaag gcttgctgac aatgcgggct
    29161 tgattggctg cctagcttta gcaataaaga aactcaatca ttagaatagt atttattaga
    29221 actattcata acataccgga agtttaaaag ggtattctga aacttaaata gtctcaattc
    29281 attcttcttt cttgagaatc tgagtagaga cttttattct gtttcaaatt aatataatta
    29341 cgaaatgata ttgagtacaa gaaagttctt tttaccagat cttttaaatc gaaaaatctg
    29401 atatatatat ttatagtact aaaaatctga ttagataaaa agctaattca ataggaaaat
    29461 aaaggtagtg aataaaagca aagcccttgt tagtagttaa tctaacaagg gctttggtag
    29521 ggaaaatggt aggaaaccat cttatttttt acgaaaatca ggaactttag taattgaata
    29581 atgttgattt ataggtattc ctcatccata ttatgttggc tctttattcc cactccactg
    29641 ttgcaggtgg tttgctggta atatcataca cgatacggtt aacgtgtgcg acttcattta
    29701 cgatacggac actgattttt tgcaagactt cccaagggat acgggcaaag tcagcggtca
    29761 tgccgtcaat agaagtgaca gcacggatac ctacagtgta gtcatacgta cggccatctc
    29821 ccataacccc aacagagcga atgcctggta ggacggtgaa gtattgccag atgtcgcgat
    29881 ctaaaccggc attggcgatt tcttcgcgca agatagcgtc tgattcacgg acgatttcta
    29941 atttttcttc tgtcaattca ccaaggacac gaatacctaa gcctggtcca gggaatggtt
    30001 ggcgccaaac gatgctgtca ggcatgccta attgggtacc taattcacgt acttcgtctt
    30061 tgaacaatgt gttcaatggc tcgatcagct tgaattgcat gtcttcaggt aatccaccta
    30121 cattgtgatg tgatttgatg gtttgggcag tttcagttcc tgattcgatc acatcggtat
    30181 aaagggttcc ttgtgcaagg aaagaaacgc cttcttcacc ggcaagtttg gttgcttcat
    30241 catcgaagac atatacaaat tcatttccaa tgattttacg tttttcttct ggatcagaca
    30301 cgccagcaag ttttgataag aaacgttctt tggcatcgac ttttataatg ttcaagccaa
    30361 attttccgcc aagactttcc ataacttgtt ctgcttcacc tttacgcaac aatacgtgat
    30421 caacgaagat acatgttaat tgatcgccga tcgctttttg taaaagaaca ccaacaacag
    30481 aggaatctac tccgcctgaa aggcctaata aaactttttt gtcaccgact tgttcacgaa
    30541 tttttgcgat ctccatgtcg atgaagttat ccattgtcca gtcaccagta cattggcaga
    30601 catctagtgt aaaatggcgc aacaagtcat ttccatattc ggaatggcga acttctgcat
    30661 ggaattggat tccgtacatt ttgcgttctg cattttggat cgatgcgatg gggcaatctt
    30721 tgctggtagc aacgacatcg aaatcagtcg gtgcttgggt aactaggtca ccatgactca
    30781 tccaaacggt ttgtttttca ggagtgtttt ggaaaagggc cgcttctgca gaagtgattt
    30841 ccaattctgc ttttccgtat tcacggtttt tggctggttt aacttttccg cctaatttat
    30901 aagtaatcaa ctgcataccg taacagattc ctaaaactgg gatccccaaa tcaaaaatct
    30961 tttcatcaat accaaaggcc ttctcatcat aaacactgtt tggtccgcca gaaaggatga
    31021 ttcctttagg gttcattgcc tttacttctt ctgcggtaat gcggtggctc atcaactctg
    31081 agaaaacacc gaactcacga atacggcgag tgatcagttg gttatactga ctaccgtaat
    31141 caagtacgat aatttttggc tcttcgtcaa atcgtgttgg tgaagacaaa tttgaataaa
    31201 acttagtccg atatgcggcg cgttctgtgc gccgcatatt tttttagttt tcaaagatca
    31261 gaccttgttg taaatagatc cgtgcccgaa agttataaaa gttgcgataa ccaaacgcaa
    31321 ttcgtttaat cagcttgatc ttgttatt
//
LOCUS       DANKHJ010000027        30759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000027 DANKHJ010000000
VERSION     DANKHJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..511)
                     /gene="recO"
                     /locus_tag="PW113_001373"
     CDS             complement(<1..511)
                     /gene="recO"
                     /locus_tag="PW113_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2525694.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSK"
     gene            complement(654..1553)
                     /gene="era"
                     /locus_tag="PW113_001374"
     CDS             complement(654..1553)
                     /gene="era"
                     /locus_tag="PW113_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2525695.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(1568..1963)
                     /locus_tag="PW113_001375"
     CDS             complement(1568..1963)
                     /locus_tag="PW113_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2525696.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(1947..2423)
                     /gene="ybeY"
                     /locus_tag="PW113_001376"
     CDS             complement(1947..2423)
                     /gene="ybeY"
                     /locus_tag="PW113_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2525697.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(2440..4632)
                     /locus_tag="PW113_001377"
     CDS             complement(2440..4632)
                     /locus_tag="PW113_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2525698.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(4656..5627)
                     /locus_tag="PW113_001378"
     CDS             complement(4656..5627)
                     /locus_tag="PW113_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2525699.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            6333..7712
                     /gene="brnQ"
                     /locus_tag="PW113_001379"
     CDS             6333..7712
                     /gene="brnQ"
                     /locus_tag="PW113_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2525700.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(7866..8312)
                     /locus_tag="PW113_001380"
     CDS             complement(7866..8312)
                     /locus_tag="PW113_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2525701.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8339..8515)
                     /locus_tag="PW113_001381"
     CDS             complement(8339..8515)
                     /locus_tag="PW113_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2525702.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(8677..9105)
                     /locus_tag="PW113_001382"
     CDS             complement(8677..9105)
                     /locus_tag="PW113_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2525703.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            9197..10057
                     /locus_tag="PW113_001383"
     CDS             9197..10057
                     /locus_tag="PW113_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2525704.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(10149..10499)
                     /locus_tag="PW113_001384"
     CDS             complement(10149..10499)
                     /locus_tag="PW113_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2525705.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(10514..12241)
                     /locus_tag="PW113_001385"
     CDS             complement(10514..12241)
                     /locus_tag="PW113_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2525706.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(12455..13381)
                     /gene="trxB"
                     /locus_tag="PW113_001386"
     CDS             complement(12455..13381)
                     /gene="trxB"
                     /locus_tag="PW113_001386"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2525707.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(13526..14959)
                     /locus_tag="PW113_001387"
     CDS             complement(13526..14959)
                     /locus_tag="PW113_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2525708.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(14959..16362)
                     /locus_tag="PW113_001388"
     CDS             complement(14959..16362)
                     /locus_tag="PW113_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2525709.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            16593..17495
                     /locus_tag="PW113_001389"
     CDS             16593..17495
                     /locus_tag="PW113_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2525710.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            complement(17535..19415)
                     /locus_tag="PW113_001390"
     CDS             complement(17535..19415)
                     /locus_tag="PW113_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2525711.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(19511..20362)
                     /locus_tag="PW113_001391"
     CDS             complement(19511..20362)
                     /locus_tag="PW113_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2525712.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(20688..22472)
                     /locus_tag="PW113_001392"
     CDS             complement(20688..22472)
                     /locus_tag="PW113_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525713.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(22472..24223)
                     /locus_tag="PW113_001393"
     CDS             complement(22472..24223)
                     /locus_tag="PW113_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2525714.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(24364..24588)
                     /locus_tag="PW113_001394"
     CDS             complement(24364..24588)
                     /locus_tag="PW113_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2525715.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            24839..26425
                     /locus_tag="PW113_001395"
     CDS             24839..26425
                     /locus_tag="PW113_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2525716.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            complement(26491..28266)
                     /locus_tag="PW113_001396"
     CDS             complement(26491..28266)
                     /locus_tag="PW113_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2525717.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(28479..29381)
                     /locus_tag="PW113_001397"
     CDS             complement(28479..29381)
                     /locus_tag="PW113_001397"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2525718.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            29660..>30759
                     /locus_tag="PW113_001398"
     CDS             29660..>30759
                     /locus_tag="PW113_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2525719.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDV"
BASE COUNT         8783 a         6556 c         5444 g         9976 t
ORIGIN      
        1 atttggaaga atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa
       61 ttggcgcgat tccgaagcgt tgcagtattt gcacttcaaa gatattcgtg atgatttctg
      121 catcatctcc ttggtccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt
      181 catattgatg atcttcgatt gctgcatcta ctagatttag caaatacgta ccataaccgc
      241 tcaaaaagat atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa
      301 acgacaaacc ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag
      361 ccggagctaa tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag
      421 aagattccgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
      481 tcaagccttt cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
      541 aagcagattc tgccgatcca gattatttat gtttcgatct tccgctgttt ttctcaacgt
      601 acggattttt atccagttta tttctatacc taacctataa gaagtccaga atatcagtac
      661 tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg
      721 actttgaccc aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt
      781 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg
      841 ctatctcttt cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc
      901 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
      961 ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct
     1021 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca
     1081 ttgtttcctt ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa
     1141 tcttcgatga ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga
     1201 taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccacgt
     1261 ttttgatcag cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct
     1321 gcttctacca taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata
     1381 aaaatcagct gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt
     1441 gccttgtcac tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt
     1501 cctacattcg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt
     1561 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga
     1621 tgacagcaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     1681 caatcggatg aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga
     1741 tgatctctac tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat
     1801 caagctggaa aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca
     1861 tattgcgttc ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca
     1921 ggaaatgttt gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct
     1981 ttctgtaaac caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata
     2041 tgcaagaatc catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct
     2101 tctgcttgtt cagcagcacg ttcaatagag atcatgatgt ctcctagtac acgaggtaat
     2161 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca
     2221 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt
     2281 gccgcattgt ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat
     2341 gctgcaaatt gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt
     2401 tcatccataa aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt
     2461 tatcttctca acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact
     2521 taaaccgctg attaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc
     2581 gtctaattga ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt
     2641 ttcgctcgtc ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac
     2701 gatccctgct tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac
     2761 ttccggattg cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg
     2821 ctgtttgcaa atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc
     2881 atctgtcaca tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg
     2941 gttttccgct cctgaaggca agttttcaac gaaaaagcta gcatgtttga tcttgccgat
     3001 atcatgataa tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc
     3061 gttagcactt agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag
     3121 caactgtttc aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc
     3181 atcattgacc atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag
     3241 tgcaccagtg aacccgcaga cgagcattaa ccaagtcgtt gcatcaccaa agctcattcc
     3301 ttgatagacg atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc
     3361 agataatcct tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa
     3421 aacataagac atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa
     3481 aagtgcggat actgcttgga aaataccagc catgatccct gctcttcgat tcaagaaaaa
     3541 gcttaaaaca agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc
     3601 cgtttggaat aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata
     3661 gaaaagcacg tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag
     3721 tacgccgatt tgaagcaaaa cagctaaaac catggcaatt agcggaaaaa tcgaagttgt
     3781 ttgattagtc agtcctagta attccaattt tttcatagct gaagcatcga tttgattacc
     3841 ttcacgaaca atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcgga
     3901 ttgtttcaat tcttctgtct ttttctcatt caatacgtca ttcactacga taccttgatc
     3961 cactaagtag cgaatcattt gttgttgtgc tggtgtgaca ttcaaatatt ggatttgctc
     4021 ttctgcttct tgtttaaaag cttccaattc actttcacga acttgtttgc tcatctgttc
     4081 gtcaatgagc ttcagactct catccctcac agtatgaatc tcagtttcag tcatttcaaa
     4141 tatcgtcgaa taaaaattgt tcggtagttt ttgataaaac acaacatttt ctgcattcac
     4201 attttcaaaa tttttcttca gcgcggcgat ccgctcttct gtagtaggct ttgggatatt
     4261 ttcatcttcc tttgccttat caatcttttc gtcataagat ttgttgatgg catcattcgt
     4321 cttatcgatc aattcaaata gttgcttgat ccgattgttc tggtcatctg caagatcttt
     4381 ttggtaagtg tattctggag tgactgcttc tgctgccagt tttctttttt gttcggtttc
     4441 ttccgtgttc tcgatcgttt tgttcgcacg gatactttct tctgctactt ggccttctct
     4501 gaaatcattg gccttttgtt tgacactact aaatgtaacg ccgcagataa tgataaaaaa
     4561 caagaacagc aggaaaggca atgctgcctt tccaagtttt tcttgtagtt tctgcagtgt
     4621 tttattcaac atgtgcttat cctcctaagc aaagcttatt cttgatggag atctgccttt
     4681 tcataagctt tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa
     4741 ttaacaaaat cgattttctt gatttgcttt aatgttcgtt ctgcatgaac aagaccactt
     4801 gtcgtccctc gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga
     4861 taaccgagac gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc
     4921 aagataacga atgcatcatc caatgttcgc ccccgcatgt aagctaaagg tgcgatttcg
     4981 attacgcctc gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca
     5041 tcataaacag gccgcagata aggatccact ttttctttta gatccccggg aagaaagcct
     5101 agattttcac ctgcttccac agcaggccgc gtcaaaatga ttttttgcac ttcacctttt
     5161 ttcaaggcag cgattgctaa tactactgca agaaaagttt ttcctgtacc ggctggtcct
     5221 acgccaaata cgatatcatg tttggctacc gattcgacat atttcttttg gccgctgttt
     5281 tttacccgta taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca
     5341 aaataatcta atgtaccttt ttgccccatt ttcaatgccg tcacaacatc gggcgtactt
     5401 acgtggattc ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa
     5461 atctgggatt gctcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc
     5521 gttgtttcct caatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca
     5581 tcagcagccg tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc
     5641 ctttttttcg attcattctt tcctatttta ccatactcag aagaaactat atagcaaata
     5701 agaaaaggtt ctaaaagagt tgcttaacca taagtaccaa gaaacgttaa aaaactagcc
     5761 gcttccattc tctaaaggtc tcgtttgaat aaaaaaagct gatcatgtaa gctatgcata
     5821 agatagagga caagtattcc gtctaactta gcaagtattc tctgggtatt tttcttgttt
     5881 caaaaaaaga aaacaaaata tttgaaatat tcttgcattt caaaaaaaac aggtgtatta
     5941 taacgtcaac aaataaatat acagcaaagc gtcgaggaaa acagtaggct attcagtgat
     6001 ttattcccag tgagtcatgt aagtgagaaa tgacaataaa tcgaatagac gaacatcatt
     6061 tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct ccattatcag gctagcggat
     6121 aactttatcg aaaagcgtcc gtgaatgaag aagctttttt aagcgaaaaa aggtggtacc
     6181 gttctagaat ctagagccct tttcccaagt ggaaaagggc tttttttata ttttaaggtg
     6241 gtggttttca tggaatctga tattagtggt atttctagtt tctgcaacgt ttattttgtt
     6301 actcaaaatg ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga
     6361 agcaattatt gatggtcagt tcacttatct tcgggatgtt cttcgggtca ggtaatctga
     6421 tctttcctgt acacttaggt caaatggctg gatcaaactg gatcagcgct ggtgtaggat
     6481 ttgccatctc aggcgctctt ttcccattat tagctatctt agctgtagta gtgacaaaaa
     6541 gtgatggtct gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttga
     6601 tccttgttca tttaacgatc ggaccattct ttggtacacc gcggacagca gcaactgctt
     6661 ttgaaatggc tgctaaaccc tttttgcctg aaaaatatga ttcattaggt atgtttttat
     6721 tctctgctct tttcttttta cttgcttatt tccttactgt acatccaaca cgtttggtca
     6781 aatatgtcgg aaagtattta aactccttgt ttcttgcatt attagctatc atttttgtcg
     6841 tttctttatt ccaacccatg gggaatctaa accaatctgc tgcagattct tatcagacga
     6901 acgctttgac aaatggcgtt ttagaaggat acaacacggt tgatgcagta gctttacttg
     6961 ctttaagtgt cacttttgtc catgcagttc gtgatcttgg ttttaaagat aaaaaagtca
     7021 cagaattaac agcaaaagct ggtagcctga gcgttgcgtt agaagtcatg atttattttg
     7081 gtctggtcct tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa
     7141 ctgcactttc tcagatcact aatttttatt taggtaactt cggtgctagc ttcttagggg
     7201 taatggtcac attaggtgtc ttcactactg ctatgggact cgttgtttct tttgcacaag
     7261 atttccataa actgttccct aaagtcagtt atatgacatg gctgcggtta acgacttttg
     7321 tctcttttgt cgttgcgaat gccggattgg ataatatcat ccaatggtct ttaccagtat
     7381 tgatgctttt gtatccattg tcacttgcgt taatcttact ttccttaaca gcaaaattct
     7441 tccaaaagag tccatttgtc tatcaagtaa cgatgttctt ttctgccgtt ccagccgtat
     7501 tggatatgtt ggcaaattcg cctgcattag tgagtcagca aactgtcgta gcaagcttac
     7561 taggatttta tcatcattat gttccttttg ctgcattagg tcttggctgg gtcgtcccaa
     7621 cgttgcttgg ttacgctggc agcttgctct tttattatgt atatcgcgta tcaggatata
     7681 aacaagaagc aagtgagtta ccagaagaat aaccaaatca aacacaattt aacataagag
     7741 atattaaaca ggaaaaaaac agctagaaga aagaataaga ttctttcttc tagctgtttt
     7801 ttagttcttt ttttacgaac acaaaaaact gcggataatc actgtccgca gtttaaatga
     7861 taaaattaag attgtaatag ttctttcaca atcgcattca cttggtttcc atctgctttt
     7921 cctttgactt taggcataat tgcacccata actttgccaa attctttcat tgatgaagca
     7981 ccagtttgag tgattgcttc ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc
     8041 ggcatatatt tttcgactat tacaatttca ctttttactt tttcagctaa atcgtcacgt
     8101 cctgcttctt caaactcatg caaagaatca cgtcgctgtt tcatttcacg agacaacacc
     8161 gttagttctt cttcttctgt caagtcgtga ccttctttaa tctgctcgtt ttgtatagat
     8221 gacttgatca ttcggatgac ttgtaaagat tccttatctt tcgctttcat cgctgctttc
     8281 atgtcatcgt ttaatctact tagtagtgac acactaccaa ctccaattca tcacagaact
     8341 agaatttttt gcgttttcta gctgcttcag attttttctt gcgtcttaca cttggttttt
     8401 cgtagaattc acgtttacga gattcttgta aagtacccgc ttttgacacg gaacgtttga
     8461 agcgacgaag agcatcgtca agtgattcgt ttttgcgaac gactgttttt gacatgttaa
     8521 ttccctccct ccgagcttaa aaagtaaccg taattgttat tgaaatatat ctcaaaaaac
     8581 aagactgtcc tgtaaatatt atatctaatg ctgttttgac cgtcaaggaa atcttgcttt
     8641 tttttaaaat tatcaaatgt tattttattt aaatccttat tgacaatttt cacatcttcc
     8701 gaaaatctcg aatctatgcc cttcgatcgt gcatccacct agctgttctt cgaaataatt
     8761 catcggacac atatggattt ctttcgttgt tccgcacact gtacagataa aatgatggtg
     8821 atggccgatt ttcccctgac agcaatgaaa acgaaacttc atttctccat ttaaatcggt
     8881 ttcttccagt aaatcgatct ctgcaaaatc atggagattc cggtaaatcg tatcataact
     8941 tacccctttg aattcctgat tcatatagtc aaaaacttct ttagcagata cgtagcggtt
     9001 acgcttcact aaataagtta acaatgcttc tcttttttta gtatatttaa aaccgttatt
     9061 tttcaatgtt tctaatgatt gttcgatcaa tgtctgtttt ttcatgactt attcctccaa
     9121 atcaaaatga ttacgaagta cgcattctag agtatggtat aataaacata aaaaaaatgc
     9181 aagtgaggtt ttatccatgt cttccgattc tacagaaatc gttacttttt ttgtcatctc
     9241 cgactctgct ggagaaactg cgacaaaact agcgcaagct actatggctc agtatcctac
     9301 cgttgagttc aacctattcc gcagaacgtt cgtgacagat gaaaaaacat taaggcaggc
     9361 attgcaagat gcattagaag aaaaagcact tgtcctgcat acattaatca atcaagaact
     9421 gatcgagata acacgttctt tttgtgaaga acatcaatta tttagttttg atgtgatgac
     9481 gccaccgata tcagaaattg aacgtttaac tggtatcaag ccaattcggc aacctggtgc
     9541 cctccattta ttgaatgaga actatttcaa acggatcaaa gcaatggaat ttgctgtaaa
     9601 atacgatgat gggaaagatc ctcgtggttt tttagaagca gatgtcgttt tgctaggcgt
     9661 atcccgaact tccaaaacgc ctttgagctt gtttttagct aacaaaaatt taaaagttgc
     9721 caatttacct ttgatcccac aagcacatat tcccaaacag ctttgggaga tcgatcctaa
     9781 aaaaatcgtg ggactaacga ataacccaga tattttaaat aatatccgta aagaacggat
     9841 gcgctcttac ggattaaacc ctgatactgc ttattccgat atcgaaaaaa ttagagctga
     9901 acttgatttt gcgaatgatc tttatgaaaa actaggatgt gtagtgatca atgtggcatc
     9961 tttatctatt gaagaaacgg cttcgcttat tttgaatgct ttagacctag aagatcatag
    10021 ttattatgga actgaaacaa cagaaaaaag cgattaatca tttttgtctt tttcttaaaa
    10081 aacactggag tgtgacaacg aagaaatttc gttgtcacac tccagtgttt atctatttgc
    10141 acctatcgtt attgatgttc cacatgtttg agtgtctggt tcaaaatatc cagtacatga
    10201 gaatcatcaa gactatacaa aatcgctttt ccctcgcgat gagacttgac gacatgattt
    10261 tctcttaata aacgtagttg atgcgataca gcagactgct ccatttccac tgcttgggtg
    10321 attgctgtca cattttgttc cccgtctttc agatatagca gaattttaag acgagtgggg
    10381 tcactcaaca ttttgaataa ttgactgact ttctctactt ttgatttttg cgtgtctggc
    10441 aatccattca cctccaactt gaaaaaagac tgacttctaa agtttaccac tttttccatc
    10501 agtctcaagt tttttattct tctgtcatct ttttgataat cgcttcataa gaggctactt
    10561 tttcggctgc gtcttctgta gagcttcctt ttacaccaat ataaaacttg attttcggct
    10621 cagttcctga agggcggata gcgatccagc cattttcttc caatgtatac ttcaatacgt
    10681 cggatggcgg tgttgttagt ttttctacgt ttccttcacg gtctgttctc gtcaattctt
    10741 taaagtcttc tgtttgaata acttttgctc cggcaaattc tgtcggtgct tctttacgga
    10801 atttttccat caaagcagag attttcgcag aaccttcttg tccgctcatt gtcacagaaa
    10861 tcgttttttc tgcgaaatag ccataggttt caaaaatcgt ttgcaagccg tcatacaacg
    10921 ttttgccttg ttttttatag taagctgcta cttctgctaa taaaagcaat gcttggatcg
    10981 cgtctttatc gcgaacaaaa ggtttaacta aataaccata actttcttcg aatccaaaca
    11041 taaaagtatg tgaatggtct tcttcaaatt gttggatttt ttcagcaatg aatttaaacc
    11101 ctgtcaacac attgaacata gtagcaccgt agtgttccgc aattgctgta ggcaattcgc
    11161 tggaaacgat tgatttcaat accgctgcgt tttctggtaa agtgcctctt gattgatgtg
    11221 cttccaaaat atattgagca aacaatgctc cgatttggtt cccagtcaat acttcataag
    11281 aaccatctgg caaacgaacc gctgcgccta aacggtctgc atcaggatct gttgcaatca
    11341 gcaagtcagc tccctctttt tctccaagtc gtatagcata ttcaaaagct gaatgttctt
    11401 ctggatttgg cgatttgacc gtagtgaaat ctggatctgc cacagcttgt tcgggaacta
    11461 gtacgaattg ttcgaatcca gcttgtttca acgcacgttc gcccaacatt ttccctgttc
    11521 catgaagtgg cgtgtacaca agtttcaaat ctttccccat tgtttcaacc aattcacgat
    11581 caatcgtcac tgctttgatt tcttctagat aagcagcgtc cacttcttct ccgatgatcg
    11641 tgatcaaccc gctatgttta gcttcttcgt cagataatac ttcgactttt agcggattgc
    11701 tgacttcacg gacatatttc gtcaatgcat ctgcgtctgc aggaggcatt tgtccgccat
    11761 cttctccgta gactttatat ccattgtaag ccgctgggtt gtgagatgcg gttaccatga
    11821 ttcccgcaaa cgtcttcaaa tgacgaactg caaaagaaag ctctggtgtt ggacgcagac
    11881 tttcgaatac ataagacggg atattgtgat gtgccagtgt tttggctgct tccattgcaa
    11941 attctggtga ctgatggcgt gagtcataag cgatagcgac acctcggcgt tttgtttctt
    12001 caccttgtgt atccatgaaa cgtgctagtc cttctgttgc ttgtcttacc gtatagatgt
    12061 tcattcgatt gatcccaggt cctaagattc cccgcatgcc tgcagtaccg aattccaacg
    12121 gtgcataaaa agcatcttca agtttttcag gatcttcacc cagatcattt aattgttttt
    12181 ttaagttttc ttctaaattt tcttcatgta cccattgttc atacacttgt tcccaagaca
    12241 tgaaaataac acctctttct gaaatatggt tcttaataag tatagcattc acttattaga
    12301 tatgaaagta tgaggaccaa aaaacgctta aatcaagaaa ggattctttt atcaagcatg
    12361 attgcaagta ttcacataaa tcggattcag tggatttcac gcgaaaaaaa agagtaaaag
    12421 aacaattgaa accgttcttt cactcttttt aaacttatgc tgttgtttct tctaaagact
    12481 ccaagtattt atacacttgt tgtccagcaa taccgccgtc accaacagct gtagtgattt
    12541 gtcgcaaatc tttttcacga acatctccga tggcaaaaac gcctggcata ttcgttttca
    12601 tttcgttgtc tgttggaatc cagccagctt cgttcgtcaa acctgcttct ctaaaaggtg
    12661 cagttaatgg atccagtcca acatagatga atactccgtc tgctgctaaa tcaaattcat
    12721 tgccggtttt gacattacgt gctttcacac ctgtcactac catttcgttt ccaacgattt
    12781 cgtcaacgac tgtatcccac ataaaggaaa ttttttcatt tgcaaatgca cggtcttgga
    12841 tgatcttttg tgcacgtaat tcatctctac ggtggacgat cactacttca gaagcaaatt
    12901 gtgtcaaata gatggcttct tctactgctg agtccccacc cccgatgact actagacgtt
    12961 tgttacggaa aaacgcacca tcacagactg cacagtaaga aacgcctcgt ccagcaaatt
    13021 cttcctcgcc aggtacgcct aatttacggt ggatacagcc tgttgcgata atgactgttt
    13081 ttccttcgta acttttatct tcacagatga cttctttatg gctgccgtga tccttgatat
    13141 cttgaacgat tccataagca ttttctgtcc caaatcttga gacactttca tacatcttat
    13201 aagctagatc ggggcccatg atcgaatcga atccaggata attttcgatt tctgccgtat
    13261 tgttcatttg gccgccaggg gcaccttgtt cgatcatcaa tacggataag tttgaacgtg
    13321 aagcatacaa agcagcagtc atcccagcag gacctgcgcc aataacgatt acatcataca
    13381 tatgtagatt ctcctttact taatcaagta agcatacttt acagccaatg cgaaaaaaag
    13441 tctattgttc tgcttgcgtt tctcgaatag ctttattata ctcgaaaaat ccgctgaaaa
    13501 acaatagact tttataaatc attgatcaat tgttcgaaag attttctcca tttgaatcaa
    13561 ttactttttt ataccagttg aatgattttt tctttgaacg ggctaatgtg ccatttcctt
    13621 ggttatcacg atcgacataa atgaatccgt accttttctt catctcaccg gtaccagctg
    13681 atactaaatc gatacatccc caagtcgtat agcctaatag atcgactccg tctagttcta
    13741 ctgcatcctt catcgcctgg atatgcgctg ccaggtaatc gattcggtaa tcatcttcca
    13801 cataaccatt ttcatctggc gtatcgactg cacctaagcc gttttctaca ataaataatg
    13861 gtttttgata acgatcataa atatcattca tcgttattct caagcctaat ggatcaattt
    13921 gccaccccca ttcacttgct tgcagatatg gatttttgac agaagcaaag atatttcctg
    13981 cagtctgttc caagagttcg ttgtcggtag tcgaaactcg tgaagaataa taagaaaaag
    14041 agataaaatc aacagtatgt tctttcagca atgcggcatc ccctggttcc atttcgatct
    14101 gaatgccatt tcgctccatt tctttcaatg cataagcggg atattctcct cgtgactgga
    14161 catcaatgaa gaaataattt tctcgatctg attttctaga agcccaaaca tcttcaggat
    14221 tgcaagtgta tggataatat gctcctgctg ccagcataca gcctactttg ttctccggat
    14281 ctacttcatg agcaatcttc gttgcgatgg cacttgcaac caactcatga tgtgccgctt
    14341 gatatttgat ttgttcttca ttttctcctt cttcaaaata cagccctgcg cccataaatg
    14401 gtgcatgaag gatcatattg atctcattga atgtcagcca atatttgaca agtcctttat
    14461 agcgattgaa gatgactcga cacagatttt cataaaaacc aacgagttct ctggaacgcc
    14521 acgcaccata ttctttgatc aaatgcattg gacaatcaaa atgcgtaatc gtcacaagtg
    14581 gctcaattcc gtgcttttgg cattctttaa agacatcctc ataaaatttc aagccagctt
    14641 cattcggctc tttctcatcc cccattggaa aaatacgact ccaggcgata gacattcgat
    14701 aagtcttgaa ccccatttct gcaaacaaag caatatcttc tttataacga tgatacatat
    14761 cgattgcaat ttttgctgga taaaaatgat cctcatcaaa ttcaaacatt tttttctttc
    14821 cggtgataac aggaaaacgg tcttttccga ttggcacaac atctacgttt gctagtccgc
    14881 gtccaccttc atcataagcg ccctcacatt gattggctgc tgtagcaccg ccccataaaa
    14941 aatcttttct gaaccccatt atacttcctc cattatatgt gtattacctg ataattctct
    15001 tgccagttca tcttgaactt cgattccttc ttcttttaat cggctgattg tttccttgaa
    15061 ttgcggtaaa tactgctggt gtgcaataaa caactcatcc atgatccgtt ttgctgtctt
    15121 tcctgatcgt ataagtggat tgatcgtaaa cgcctgtaaa gctgtcccat aatcaccagt
    15181 cacagctgct tcgatcgtca gcaattccat attcttcatt acttgcaacc agcctctttc
    15241 agcagcagga agagaaccaa acgctacatt tctcgctcct tgtgcgccaa cataggcagt
    15301 gacttctaca acacaatctg ccgacaggtc aggaacagct ccatcattct ttgtagatac
    15361 aacgatctgt gtttctttat ttccatagat tgaagcaatc gtttcacagg cagcgtctga
    15421 ataataagcg ccacctcgtt tttgcaattg ttcaggttta tggtctaatt ctggatcttt
    15481 gtataattca aatagctctg cttctgtttc tttcacttgc tgcgctcttg taccaatcgt
    15541 ttgatattct tctaaagcat gttccagcat ttcttcttca cgatagtaat agcggtggta
    15601 gccacaaggg atcatgttca tttgttttaa ttgttctttg aaaaacggga tatcaaagat
    15661 atttttcggt aatccattat cttcatcgta taaacgatca atcaattctg ccgtgcggtc
    15721 atttccttta ttatctgcta ctttgtgcca atggaaatga tttagaccgg caaacttgta
    15781 gatcagctct tcttttttct tgccaagcat tgctggttct gtcatcatcg ccatcactgg
    15841 cacattgcat aatccaatca ctttatccca tttgccatat ctgataactg cttctgtcac
    15901 catcccactt gggttcgcga aattgatcag ccaagcattt ggacataaac gcttcatgtc
    15961 ctcaacgatt gccaaaacga ctggaacggt acgaaaagct ttgaatatcc cgccagcacc
    16021 atttgtttct tgcccaatca tcccataata actagggatc cgctcgtctt tgattcgtgc
    16081 atctaattga ccgacacgaa actgagtcgt aacaaaatcc gcatctttca atgcttcttc
    16141 ccgatccaga gttgcataga tcttgacttc atatggagaa gcatcccaca tacgctgaga
    16201 catctgacta acgatctcaa gcttttcttt tccttcttct acatctacga gccagatttc
    16261 tcgtatcggt aattcctcat atcttttgat aaatccttcc atcaactcag gggtgtaact
    16321 gctgcctcca ccaatcgtag cgattttgat tccttttgcc atgttctcac tcctttactt
    16381 ttttacactt tcattatctt ttttgtaaac aaaacaaaca atagaaatcc gtttctttcc
    16441 agtaacattt acaaaagtta ctgttttcta gtggattttt gtaaatatct tgtcaaaaaa
    16501 agcctatttt aagttatata gtataaatcc gtttacaaaa cattttcaaa aaagttataa
    16561 tttacgtaaa aaaataagaa gaaggtatct acatgttatt acgtgaaaaa atcaaagaaa
    16621 tctccttttc tccttcagaa aatgaagtga tccagtttct cttaagcgat tctccagcac
    16681 ttggcgaaat gaccatccaa gcgatagctg agcgatgtta tgtccacccg tctacactca
    16741 tccgtgtagc aaaaaaaatg ggttttgatg gctgggtcag ttttcgtaat gcttttttag
    16801 ctgaaacagc ctatcttgaa gggaattttc aagacgtaga tgcgaatctt cctttttcca
    16861 ccacagacgg tattatgaca atcgcaaata aaatggcttc tttagaacag atgacgattc
    16921 atgatacact ttctctcatt catcatgacc aactgcaaaa agctaaatat ttactgctcc
    16981 aggcaaaaaa aatcgtgatc ttcgccagta actcgaatac tttgatttct caagatttca
    17041 tgctgaaaat gaagcgcatc aaaatggatg tcacaattgt caccactttt ggtgaatcat
    17101 tctatgaagc gtacaattgt acgagcgata tgtgcgggat tctgatttct tatacgggtg
    17161 aaaacaatgt gatccttcgt accgcagaaa tattgaaaca aaatgatgtt ccaattttag
    17221 ctttgaccag tatcggtgat tcaacgctat ctgcaatgag tacagccgct ttgcgactta
    17281 cgacacgcga gcgactgtat tcaaaaattg gtaattttac gatcaataca tcaatttgtt
    17341 atcttctaga cgtgttatat agttgtgttt ttgcagaaaa ttatcaacag aatttagtcc
    17401 atctaatttc tgtcggtcaa aaattcgata aacgccctat cagctcagcc atcatgaaag
    17461 agcctttcaa ttttgaaaat aaagaagtgg aataaaaaga atagttaagc ctagtccatt
    17521 cttcacctaa ccgttcaggt cagtgcggtg ataagtgcat cgtctggccc gaccgtctcc
    17581 ccttggtttt ccaaaatatc caaataatcc gtgtaattgg tgacgatcac aggtgtttct
    17641 aaactgtatc cttccttttc gattttcttt ttgtcgaagc gtagcaacag atcgccttgt
    17701 ctcactcttt ctccttgctg cactaagctt tcaaaatatt tcccttccaa ctgcacagta
    17761 tccaatccga catgaatcag caattccagc cctgtttcag aaaccattcc gattgcatgt
    17821 ttcgtcggga acagggtcat aactgttccg tcaaaagggg cacgtacttc tcctttttca
    17881 gggtagatta agattcctcg acctagtgtt ccttgtgcaa aagctgcatc cttcgctgca
    17941 tttagtggag cgatttgtcc ttgtatcggt gtttttacaa tttctttacc tgattttttc
    18001 tgtatagcat ctgtttcacc tgcttcatcc ttccaaaaga aaaaagtcag cgaaaacccg
    18061 attaccgaag caatagcaat gccgataaac gcttgaaaca taccactggc gtctgtttcc
    18121 gtgatgtagt ttaccacccc aaaaatccct aatccgccca ttatgtagct gattgttcca
    18181 gatgccatca aatagattcc tgaaacagca ccaccaatca tcgagtaaat aaatggcttc
    18241 ttcttcggta aggtgattcc ataaatagcc ggttctgtta cgccgaagat tcctgaaatc
    18301 acagctggca tgcaaagcgc cttcattttt tgatccttga acttgaaata catcgccaat
    18361 accactgctg tctgtgcaaa actcgcgcca taaacgccta ctaacatctg actccaccca
    18421 ttttgcgcta gctgcataat cgcaagaggg ataatactcc aatgaagtcc gaaaatcacc
    18481 agtacttgcc aaagaaaccc tagaactgca ccatacacag ctggagaaaa gtccatcaat
    18541 gcttggaatc ccgtgcttaa tagatctgtc agtatactga caatcggacc gataatcaaa
    18601 aagccagcag gaagtgcgat taataacaca gaaaaagaaa ccaaaaaggt ctggacgact
    18661 gcaggaatcc atttcttggc aattttttgg acttgtccgg caaatgcgat aatgaagata
    18721 acaggtacga cgctactagt ataactgctg cccacccatg ggatacccaa aaatgtttca
    18781 taagccggaa gacccagcaa gctataaggc gcaggactgc catcagggaa accactttgt
    18841 agtgctgctc cttgtacagt cggataacaa agtgctgcac caatcacgag ccccaccatc
    18901 ggattcagat gaaatttttt cgctgctgtg taccctaaga tcactggcat gaaataaaaa
    18961 attgcatccc ctatcccatt cagcatgatg tacgtacccg atccttcagt ataaaatttg
    19021 agaaacaaga acaaagcatt taatcctttg accattcccg ctgcagccaa agcacctaaa
    19081 aaaggctgga aacagccact taatatgtcg attaatcgat tgaacagatt actacttgaa
    19141 gaaacctctt cttctgcttc atcggaaatc ccggctactg tcagtacttc tgcatagaca
    19201 tctggcacat gattcccgat tacaacttga tattgcccgc cgcttttcat gatggtgact
    19261 atcccttcca tctttttcaa aacatcatcc tttgctttat tttcctcctt tagtttgaaa
    19321 cgaagtcgcg tcacacagtg tgtcaagcta cggatatttt cttttcctcc gatattcttt
    19381 acgatgtttt ctgctaattc ctggtatttt cccatttcta ttccctccaa ttaattagtg
    19441 gaaggtttag ccaactgaat gtcactaccc ttatttcata cgttcttgtt aatttgtcct
    19501 ccttttttat ttatttacaa cgattctcat gcactaaacg cgcagtatgg atcgttaaat
    19561 acattttttc atccgtggac atctggtagt cgaaagcccc ggttaaaaag tctgctattt
    19621 tctcaacgca accaaacgta tccttatatt ttttttgaat aacttggaac aattcctcag
    19681 aaacttctcc atcttctgat ttcgctccat ctaggacacg tgtcagaaaa aaacgcaggt
    19741 gtgtgacgaa tcgaaaaaag tggactgaat cttcatcaaa tgtaatctta tagtaatagc
    19801 ggacgatatt taaaatatcc tgtattgttg tggtaaacag gtaggtattg tcttgctggc
    19861 tttgaatgcc ttgtgcattg actaaatgca atgcgataaa tccggcttcg tcttctgata
    19921 acttgatgtg gtatttatcc gagattttct tcactgtttt tttcccaatg gcatactctt
    19981 taggaaacag ttgtttgata tcatacagca atacatttgg cacaaagatc cccatttttt
    20041 gacgttcgat agctgtatac aagtgatccg tcaatgaaat atacacagaa tcatttaaat
    20101 ggacttgcag agcctctttt gtatattcaa tgatttcaca agaaatttct aagtactcaa
    20161 gaggaatcgc ttgtgccaat tcttggaact gttgtgaaag tccgtcattt gtcaactgaa
    20221 attttttgtc gatgcatgag tcaagaatcg ggtcacccac cttctttttg aatgccaatc
    20281 cttttcccat cacaatcatt tcccgatctc tttcatccct tgttattacg acgttgttat
    20341 tcagtacttt ctcaatcagc atgttttgct cctttccatg atatataggg ctgtatccaa
    20401 aacaaaaaga aaaacccaaa aagccgtcca tgaaaccatg ggagactcat caggtttagc
    20461 ttgcatcagg caatcactat ccaagttgta tcgttttctt tatattatcg aaagcaaacg
    20521 attttgtcaa cgctttctta ggggctatta ggcacttctg actagctgac gtcgtctttt
    20581 ttaaatctgg taactacgac actaactctc acagatgaaa aatatgggaa gctatgtttt
    20641 attttcactc tagtgcttag gtcaaaatgc tactgtcaca acatctttta tataccacta
    20701 ctttgcagtt tgtacatatc tgcatacaaa ccattttgcg ctagaagtgt ttcatgattc
    20761 ccgcgttcta ctatatgacc tttgtctaaa actaaaatca attctgcatc tcgaatcgtt
    20821 gacagccgat gggcaatcgc aatcgttgtc cgtccttgtc gcattttcgc caatccttct
    20881 tggatcaatc cttctgtttc agtatcgata tttgctgttg cttcatccaa tactagaatt
    20941 ttaggatcag tcacgattgt tcgcgcaaaa gtgatcagct gtcgttgtcc actagaatag
    21001 ctggcaccat gttcgatcac ttttgagtga tatccttttg gtaattcatc gatgaatttg
    21061 tttgcttgaa caaactcagc cgcatggcgt atctgttcgt ctgtgatagc tggattcatc
    21121 atccgaatat ttcctgcgat atccccataa aacaagaaag catcttgcaa gaccagcccc
    21181 attttcttac gtaactcttc cattggatat tctcgaatgt catgatcatc aatcaatatc
    21241 tgaccttcat aaaattcgta aaaccgcatc aacacattga tgatcgagct tttcccactt
    21301 ccggtgtgtc ccactaatgc aacggtttct cccggattag caacaaaaga gatattgttt
    21361 aatacattgt gctctccatc ataagaaaag cttacattgc gaaactcaat cttacctgaa
    21421 gtaatcattt gatctgctcc agggttttgt tgtggtgcat attcttccgt atccataatc
    21481 ttcaagatac gactaccagc aaccatccca tctgtgaaga tgctcaaaaa gtccatcatt
    21541 tgagacattg gattaaaaaa tccctggaca taagtcgtaa atgcatagat cagtccaact
    21601 tctacgggag aatgcaacgc atcgatccca aataaagtca acgccaacgc aatcgccaat
    21661 gcatatagaa aattgatgat cggacttaac agaatcgaat tcgttttgat catcgcatag
    21721 cgcgtgtcta aatactcatt gttcacttgt tcaaattctt tagctaaacg agcttcttgg
    21781 cgaaactgct ggataatacg catgccggaa atcgattcat tcaattttgt attcagctga
    21841 cttaattttt cccgcatatt tcggtaaata cgagaactaa atttctggta ataccagatg
    21901 acaattccta aaatcggcaa gaacaccaaa gtccacaagg caatttctcc attgatttga
    21961 aacatggcaa tgaatgaaga aacgactgca aagatcccag tcaataccat caaaaagacg
    22021 taccaaaatt caaatagcgt ttctgtgtca ttcgtcactc gagaaacggt tgatcctgcc
    22081 ggtgtctgat caaaataacg catacctagt gtatgcagct tttcaaataa ttttacccta
    22141 atatactgat aggtcttcaa tgaagccatg gaatacaaat accattggaa aaaccaaatg
    22201 atacttttta ctaggactcc aaataagtac ataccagcaa aaaacaaaat aacttgtgtc
    22261 gtagccgact gtttagccag atgatcatcc ataaaagttt ggatgatcct tggcaatagg
    22321 acattgatca cagacagagc aaacgcaaat aagatagcaa cgatgaacgt tgttctaaaa
    22381 ggtttggcga acttcatcag tcgtttgatg atctttttct gttctttaag tggaatcgat
    22441 tttgtccatt ccgattgata attttcatcc atcaggcctc gcctccttct atcttcgctt
    22501 ctaattgctg tttctcccac atttgttcat accagccgcc tagtgcaagc agctcatgat
    22561 gggttcctcg ttcaatgatt ttcccttgat cgacaacaat gatttctttt gcatgcatca
    22621 cgctacttaa acggtgagct gcaatgatag ttgttttctc ttttcttatt tcttttaaat
    22681 tatgcaagat agcttcttct gttttggcat ctactgcaga aagtgcatca tctaatatta
    22741 ataattcagg atttttgatc aatgctcgag caatcgaaat cctttgtttc tgtccgccag
    22801 ataaagagac tccacgttct ccaactaagg tatcataacc ttttggtaat tccttgattt
    22861 cattgttgat aaatgctaaa gaagccgcat gctctacttc ttcttctgaa agttttggat
    22921 tagaaaaacg gatattatct cgaatggtca tcgaaaacaa gaagtgatcc tgaggcacat
    22981 agccgattga tcctaataaa gcattcaatg aatattcttt gatcgaacgt ccgccaaatt
    23041 ggattttccc ttgataatca tcgtattcac gcatcagtaa tttgatgatc gttgtttttc
    23101 ccgcaccagt tttcccaaca atacctaatg tttctccttc ttggattgca aacttgattt
    23161 gttctaatgc tggtgattct tctttcggat aattaaaaga ggaaatagct acattcagct
    23221 cgccttttac cggggtctgc actgcaccca gttgttccac gatatgcgtt ttttcatgca
    23281 aaagttcatt gacgcgatca taagaagcat ttcctctttc caatacgttg aacaatcgac
    23341 caatcgcaaa catcggccaa acaagcatac cgatataact aataaatgag accatctgcc
    23401 cgatcgagat cgtgccttcc aagacatact ttccacctaa aataattgtt gcaacataag
    23461 acagaccgat aatcaaggta atgaatggat caaataagga gtctagaaaa ttgactcgtt
    23521 tgtttttaat aattgcatct tcaatctttt cttcaaaatc cgctagatct tctttttctt
    23581 gtccaaatgt tttaatgact ttgatcccag tgatactttc ttgtgtctta tcattgatac
    23641 tagaaaaagc ttcttgcgaa tcacgaaaag catcatggag tttcgacccc agcactcgtg
    23701 aagtgacggc cagcaaaggc agaggaatca gcgcaatcag tgttaagcgc caatcaataa
    23761 acaaaatcat cacaatgatc gttgttccgc ccgtaatcac tgaatctgca aaggtcaaga
    23821 taccagctcc tgctacattc tggatcgcat tcaaatcatt cgttgcatga gccatcaaat
    23881 ccccagtccg atatttttgg taaaagagat tgtccatttt tgtaaaatgt ttgaataact
    23941 gctgccgaag ttctttctcc aaccgagctg cacttcccca aatattcgtc cgccagatat
    24001 aacgaaaaag atattgtaag aaagcagcga taatcaatag gaagatccac ccgaaaatga
    24061 tctttactga taaattttta tcggcaatct catcaatgat gatcccaatc acttttggag
    24121 gaactaactg taccaaggcc actgcgaata gcgagaaaac cccaattaaa taatgtttct
    24181 tttcctgacg aaaaaaccag cctaactttc taaaaatcga cattttactc ctcctttgtc
    24241 tttatcaaga gcctaggatt gtctgtgctt gtctttttgc tgaataatct gaatagtcta
    24301 acaaattaca aaataaaaaa cggactgaaa aaatcagccc gtaaaaagtc gttgggtcag
    24361 aacctatttt ttcccttggt tcttcatttg ctgcatcatc tggcgaattt tcttctcaga
    24421 tggtttttgt cccattgaca tcatcattga tcgcaacatg tcctcattaa cgggaggatt
    24481 tttttctaaa tagtctttca tatatttacg cgctaggaaa aagccaccta ctgcgccgat
    24541 tagcaaagca ataatagcaa taagtacgac aatacctgtt gataacatat aaatctctcc
    24601 tttctatttc cgaattgctc tcaatgactt attttactaa agtttattga aaaaagaaag
    24661 gcgtattctt tagtttcatg caaaaaatag tggttttttt agaggagaac aaaatttctt
    24721 tcgtcatcta acaagacgcg ccacgaaaat aaattaggca taccgaatat ttttctttaa
    24781 ttttttcttt ttgaggtgta gaatgtaatc gtagataaat aggaaaggtg gttttttctt
    24841 gaataatcaa tccaacaaac aatttcataa gcatcgactt attgaacata caaatggttt
    24901 atctttagct gaagtaaatg gaacagttga tgtaccaaag gggaaaagtt tttttaaaac
    24961 acttcttgct tactctggac caggtgcatt agtagcagtt ggttatatgg atccaggtaa
    25021 ctggtccact tcgatcactg gtggacaaaa tttccaatat ttattgatgt cagttatttt
    25081 gatgtctagt ctgattgcta tgcttttaca gtatatggca gcaaaattag gaatcgtgac
    25141 ccaaatggat ttggcccaag caatccgagc acgcacaagt aagacattgg gtgtcatttt
    25201 atggatcttg actgaattag ccattatggc aacagacatc gcagaagtca ttggtgcagc
    25261 aatcgccctc tacttacttt ttgatattcc attggtgatt gccgtcttta ttactgtatt
    25321 agatgttttc gttctcttac tactgacaaa agtcgggttt cgtaagatcg aagcactggt
    25381 tgtctgctta attttcgtca ttttattcgt ttttgtctat caagttggct tgtctaaccc
    25441 agattggcac agtttatttg ctggctttat ccctaacagt catacattct cagactcacc
    25501 ggtcatcggc ggcgaaactc cgctgactgg agctttaggg attattggtg caacagtcat
    25561 gccgcataac ttatatcttc attcagcagt ttctcaaact cggaagattg atcaccaaga
    25621 cgaagaagat gtagcacaag ctgtacgttt ttctacttgg gactctaata tccaattgtc
    25681 gatggctttt ctagtcaatg cactgctatt gatcatgggt gtcgctgtct tcaaaaccgg
    25741 ttctgtccaa gatccttcct tttttggtct gtatgaagcc ttatctgatc catcagctat
    25801 gagcaatggt atcttgattg ctgtagcaaa atctggtatc ttatccactt tatttgctgt
    25861 agcacttctt gcttctggcc agaactctac tattactggt acgttgaccg gtcaagtcat
    25921 catggaaggc ttcatccata tgcgtatccc gatttggcta agaagactgg tcacacgatt
    25981 gctttctgtg attcctgtat tgatttgtgt tttgtttaca agtacaaaag gaacgatcga
    26041 agaacatacc gctttgaata acttgatgaa tgaatcccag gtgttcttgg cttttgcgtt
    26101 gccgttttcg atgatccctt tactgatgat gaccaacagc caagcggaga tgggtagccg
    26161 atttaaaaat tcattcatcg taaaacttct tggctggttc tctgtgatta gtctaactta
    26221 cttgaactta cgaggattac caggacagat cgaaagtttc tttggtgaac aagccacttc
    26281 tgctcaacta gcattggctg atcacatcgc ttatgtaatc attgttcttg tccttctcct
    26341 gttggcatgg acgattggtg atttatataa aggaaacaaa aaatatgaac agcgtttgca
    26401 ggaaacggta gaagaaatcg aatagaaact gatttttcca cacaaagacc cttgatagtt
    26461 aggttaaaaa tctagctacc aagggtcttt ttatttagaa gataaagcct ttttcagatt
    26521 taatttactg aaaactagca tgcttgttac tacgatgaat tgcgttaatg gcacgcctgc
    26581 gccagtgtag acacttaaaa aataatcctt tttttctggc gcataagaac aaaaccacga
    26641 gacaccgaaa agaaaaacca attgtgtcag tattaatccg gtgaccacaa tcaaagagga
    26701 actataatag gcacgatcag cttttgcaaa tacggttcct agtatcataa gtaatatccc
    26761 tgctattgaa gcaatcagca cgggaatagc cagaacttgg taaattttta tgatgacatt
    26821 cgcgactttt gctgattttg ttaattggaa attaggtgtt ctcggttcat tactatcgat
    26881 ccaattctcc attagcggaa ggtttaataa ttcttgagcc tgtttgactt gttcttgcgt
    26941 tccgaaacta taatttcctc cttgatggaa atctttatat cgccatgcat aagcgtatcc
    27001 tgattgaagg aaatcgaata cgattggcag atcactcatc gtctttccat ctcctgatcc
    27061 catcagatag aactcttttt tctttttgat ggttcccttg tgataggctt cttctaattc
    27121 ttggttgact tttttccaaa aatcattggt ttcttttgca ttgttttggt aataaccaga
    27181 ttcgcttacc acatctcgca atgcccaaaa gatgatatct ccgggtaatt cttttacttt
    27241 tccaccttga gtccacgcgt gagtcgtata taattcgtca atacgggtag aaacagtatg
    27301 gaatgtagga gatgcggcta atgctttatt tagtgcagca tgtgagaccc atatctcact
    27361 gttttcttta ttcaagttcg tttcatcatc tattcggatc agttgtttca tcaaacgtcc
    27421 aaattctcca ctggaacggt cattgatggt tgcaataccg tatgttgtct catttttata
    27481 ttcgatccac tgatagacga tggaaaagcc aactatagga agtaaaaaca atgaaagttt
    27541 cagccattcc cgtttcgaga gaagaaagag tcgttttttt aacttcttta cttctttgaa
    27601 agaaaagtct tgccctagca agatctggac gaaactgata cccaaagcta ctacagtaaa
    27661 aggaagaagc cagatagaat cttcgcgaat gaaccaaaag aaaggaaaag cgacagaaag
    27721 ccctaaaaac catggcagca attttctgct agaaccgtat ttgcggtaat aaatagctag
    27781 taaacaagcg ataatgatct caacagccgg aatcacgagg gcattacgat atgttcgagt
    27841 ggaatattca tgtgtaaaac taatcggaga aaacaaaaaa taaaaataga tacttaaaag
    27901 aagatacttg ttcttcacga tcggtttaaa cgcgagtgca accaatatac tggcaaaaat
    27961 actgaacatg ccgaacaaaa cagcataaga aagatgcagt ttatgtgcaa gtactaaaaa
    28021 taagctgtag gagattcctt tggaaagtgt tgtagtcgta tactccccta gccatttacc
    28081 gtccaaaata ttttgagcat agcgaatcag cagctggtca tcataggttg ccgacatgtc
    28141 tgttccatag attgctttac tatataaata caaacgaata attgtaaata atagggctat
    28201 gccaagaata aagtatgtat gtttcttttt tagttggttg atcaagggtt ttaaagtgtt
    28261 cagcatcttt tcgtccttcc aatcacgttt tgccacttgc ttgtgaaact tttcgttttt
    28321 ttacttttct accagtatac cctgtttgtc taaatttaaa aagagggggg agaagatgca
    28381 tgatgaaaaa gactgcaaaa agcactcctt tgtttcttaa acaaaaaagg acaaggcaat
    28441 gtgcctggtc cttttttgct gctgtcagat agaaaatctt agagtatcac ttcttctgct
    28501 aataactcag gaaacacttc tggatcgaat atttgtgctt tgaacatggc gatttctttt
    28561 ccatatggtg cgtaagcttt ctttttatct ttgccgacat agggagtttc caagattttc
    28621 gaaatattgt caaacattgg atcatggacg atcttattca gcgtgtcgaa tccgattgtt
    28681 ccaaaaccaa tattggcgtg ccggtcttta tgcgaacctt gcggattttt agaatcattg
    28741 atatggagta ctttaagccg atctaacccg atgatcttat caaactcttc cattacacct
    28801 gaaaagtctt ctttaatatt ataaccggca tcattcgtat gacaggtgtc aaaagtaacg
    28861 gataattttt catttaattt tactccatcg atgatttgcg ctaattcctc aaatgttcga
    28921 ccgatttccg tacctttacc tgccattgtc tccaaagcga tttgtgccgt ctgttctttt
    28981 gtcaaaactt cattcaagcc ttcaataatt ctagcaattc cggcttctgt tcctgcccct
    29041 acatgagcac ctgggtgaag tgtaatttga gtagcgccta aagcttccgc acgaacaatc
    29101 tcttctctca gaaattggac ggcaaaagga aacatttctg gtttgatcgt attccccaaa
    29161 ttaatgatat aaggggcatg aacgacgata tttgttagac cgttttctgc catgtacgct
    29221 tttccggcat caatattcat ctcttcgatg gcttttcttc tagtgttttg aggggcgcct
    29281 gtatagatca taaaagtcga agatccataa ctggctgctt ctgctgctgc tcctaacagc
    29341 attttctttc cgctcatgct gacgtgtgag cctaataaca tggatggatt cctccttgaa
    29401 aggcaattgt tactaacaat tcctatatat tggtaagatg atatacaaag aaaatgattg
    29461 atttttcttt gccctgttct ccatagtaac tgaaattttc tctaaagaaa agcttaagaa
    29521 ctcttttata ataaagatca ttttctcacg ttttaactct tttaaaaaag aaattcctgc
    29581 caaaaacagc cttgtctgtt tttttgttct attatttgct ataattagcg aaataaaacc
    29641 attagttgag gtgacccttt tgccacgcaa aaatactcga aaaaaacaga atagaagaaa
    29701 aaaagaaaaa tggtttgttc ctaagattat ttttagagtt tttcaatcat tgactgtttt
    29761 tattactgtt cttcttttga tgttcgcagc tctaggtatt ggaataggcg ctggctattt
    29821 tgcctatctc gtcgaagaca cacaattgcc gactaaaaaa gcactgcaga cagaactagg
    29881 aaacattaca gaaacgtcaa agattgttta tgcagataat acagagatat caaaaatcca
    29941 gacagatctg atgcgcacga ctatttcatc tgataagatt tcacctttat tgaaaactgc
    30001 aattatcagc acagaggatg aatacttcga taaacaccaa ggttacgttc ctaaagctgt
    30061 tttacgagca ttagtatctg aagctactgg tattggctct tctggtggat caactttgac
    30121 acagcaatta gtcaaacagc agatccttac agatgaaaca acattcaaac gtaaagcaaa
    30181 cgagatcatt ctttctgcac aagtagaaaa atacttttca aaagatgaga tcattgctac
    30241 gtatttgaat gtctcaccat ttggtcgtaa taataaggga caaaacattg ctggagtcca
    30301 ggaagctgca aaaggaatct ttggtgtaga tgcaaaagat gtgacgctgc cgcaagctgc
    30361 ttatatcgct ggattacccc aaagcccgat cacgtatagt ccatacacga acacaggcgc
    30421 gctaaaagaa gatctttctg ccggccttgc cagaaaagat tttgttttat tcagtatgta
    30481 tcgtgaaggc caaattacga aagaacaata cgaagaagca aaagcctatg accttacaaa
    30541 agactttcta ccacaacaaa tcgcagaaca gactgacaga gaattcttgt attatactgt
    30601 gatgaatgaa gcaacaagaa tcattgcaca acaattggct gaaaaagaca acgctgatat
    30661 gtctgattca tcggtcagtg acgcttatta tcaaaaggca cagcaaacga tccaaaataa
    30721 aggatataca atccattcga caatcgataa agatgtcta
//
LOCUS       DANKHJ010000028        30203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000028 DANKHJ010000000
VERSION     DANKHJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(499..1443)
                     /locus_tag="PW113_001399"
     CDS             complement(499..1443)
                     /locus_tag="PW113_001399"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2525720.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1739..2683)
                     /locus_tag="PW113_001400"
     CDS             complement(1739..2683)
                     /locus_tag="PW113_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525721.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2904..4118
                     /locus_tag="PW113_001401"
     CDS             2904..4118
                     /locus_tag="PW113_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2525722.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4140..6155
                     /locus_tag="PW113_001402"
     CDS             4140..6155
                     /locus_tag="PW113_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2525723.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6238..7611)
                     /locus_tag="PW113_001403"
     CDS             complement(6238..7611)
                     /locus_tag="PW113_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2525724.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7637..7921)
                     /locus_tag="PW113_001404"
     CDS             complement(7637..7921)
                     /locus_tag="PW113_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2525725.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7923..9938)
                     /locus_tag="PW113_001405"
     CDS             complement(7923..9938)
                     /locus_tag="PW113_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2525726.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10117..10422)
                     /locus_tag="PW113_001406"
     CDS             complement(10117..10422)
                     /locus_tag="PW113_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2525727.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10511..11542)
                     /locus_tag="PW113_001407"
     CDS             complement(10511..11542)
                     /locus_tag="PW113_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2525728.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11749..12624
                     /locus_tag="PW113_001408"
     CDS             11749..12624
                     /locus_tag="PW113_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2525729.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12776..13618)
                     /locus_tag="PW113_001409"
     CDS             complement(12776..13618)
                     /locus_tag="PW113_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2525730.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13710..15587)
                     /locus_tag="PW113_001410"
     CDS             complement(13710..15587)
                     /locus_tag="PW113_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2525731.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15584..17005)
                     /locus_tag="PW113_001411"
     CDS             complement(15584..17005)
                     /locus_tag="PW113_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2525732.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17008..20397)
                     /locus_tag="PW113_001412"
     CDS             complement(17008..20397)
                     /locus_tag="PW113_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2525733.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20654..22045
                     /locus_tag="PW113_001413"
     CDS             20654..22045
                     /locus_tag="PW113_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525734.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22118..22765)
                     /locus_tag="PW113_001414"
     CDS             complement(22118..22765)
                     /locus_tag="PW113_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2525735.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22773..23459)
                     /locus_tag="PW113_001415"
     CDS             complement(22773..23459)
                     /locus_tag="PW113_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525736.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23465..24538)
                     /locus_tag="PW113_001416"
     CDS             complement(23465..24538)
                     /locus_tag="PW113_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2525737.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24760..25434
                     /locus_tag="PW113_001417"
     CDS             24760..25434
                     /locus_tag="PW113_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2525738.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25439..26779
                     /locus_tag="PW113_001418"
     CDS             25439..26779
                     /locus_tag="PW113_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2525739.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26928..27914)
                     /locus_tag="PW113_001419"
     CDS             complement(26928..27914)
                     /locus_tag="PW113_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2525740.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28638..29669
                     /locus_tag="PW113_001420"
     CDS             28638..29669
                     /locus_tag="PW113_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2525741.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29778..>30203
                     /locus_tag="PW113_001421"
     CDS             29778..>30203
                     /locus_tag="PW113_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2525742.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8962 a         5643 c         5417 g        10181 t
ORIGIN      
        1 tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata atatatatta
       61 tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc aaaaatcgtc
      121 atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt ttttcttact
      181 ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa tcaaatatga
      241 acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa ttaaccacag
      301 ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat atgttcatat
      361 gtacggatac attttcggac cgattaattt taaccattct atttatttgc ttacctatta
      421 gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt aggattttta
      481 gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag cacttgttga
      541 tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt cacgaacacc
      601 attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt attcaccagt
      661 taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt ttacgatacg
      721 tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga tctcataagc
      781 agcattacgg actttatcag caatcacatc taattcatct tgggcaatac gatggtcttt
      841 ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg cagcaacttc
      901 tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa cttttaattt
      961 caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac cgataacacg
     1021 tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta ctgggtttga
     1081 agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga tttggcgcat
     1141 gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt ggttgatacc
     1201 agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg accatacagc
     1261 cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc cttctgcttt
     1321 ttcttgattc acatcgatca atactaattc attagagatt ccttggttga tcattgcata
     1381 agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac ttgttttttt
     1441 catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt atagtcttgt
     1501 ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt gatggaaacg
     1561 aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc ccctgataat
     1621 acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac gatataataa
     1681 gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga ttttcttctt
     1741 aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat aatttgaata
     1801 aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa aaactcgttt
     1861 cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct tgaaaggaag
     1921 catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca aaatgagctg
     1981 ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat actctaagca
     2041 tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat aaatgagtca
     2101 attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga ctgtatgtat
     2161 caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc
     2221 gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc aaaaaagaag
     2281 aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag gtttcttctt
     2341 ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt tgagtagctt
     2401 ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca ttaataattt
     2461 ttaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta tgctcgggaa
     2521 cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat agatcccctc
     2581 ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg atattcattc
     2641 cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat gtctaattca
     2701 gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat tagttcacgc
     2761 tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta tggttgggac
     2821 atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata tttttgataa
     2881 gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa ttttctctgc
     2941 tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt cccagtatgt
     3001 cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact gctccctctt
     3061 ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc ggacaaaaac
     3121 aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc ctcttctcac
     3181 aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt ggattgatca
     3241 attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga gcctctatga
     3301 ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt gttgcaggac
     3361 agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc ttacctattc
     3421 ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa tcgcatggca
     3481 cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat ggtatttttc
     3541 tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca gtcctgacga
     3601 cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg caattggcaa
     3661 gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc tatatgctta
     3721 cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg ataaacctgt
     3781 atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc attccttact
     3841 tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat acttcaatct
     3901 tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc tttctctctt
     3961 ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc ttgctgttga
     4021 ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca gtgataagag
     4081 tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg tcgtgtaaaa
     4141 tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt gagatccgcc
     4201 atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca gcgtactggt
     4261 acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt gatcaacctt
     4321 ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga caaaaacatc
     4381 catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc tttgtaggac
     4441 atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca gatattggct
     4501 ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt tatgtcgata
     4561 tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca gtcactgaaa
     4621 cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc tgtgtagaac
     4681 taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag tccgatgtag
     4741 aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga aaaatgaaag
     4801 ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga atggttcaac
     4861 ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca agcgattctt
     4921 ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt agtgatttgt
     4981 tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg tatcaatcat
     5041 tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg atattagcat
     5101 taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat tcgatcttgt
     5161 ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt ttacgcatcg
     5221 tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat gaattagggt
     5281 ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg gataaagaag
     5341 gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg atcacttgcg
     5401 gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat ctctatcaag
     5461 aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa gaacttggcg
     5521 cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg aatattttcg
     5581 ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag aatgtcttat
     5641 tgaatgacac tatcacacag attactactc catatttcaa attctatgaa caagatgctt
     5701 tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat tggggaggga
     5761 tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa acaggaaatg
     5821 aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca tggggagcaa
     5881 gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag ccaggttatg
     5941 aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt acgctaccta
     6001 tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt acttctgata
     6061 gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca aaacagccac
     6121 ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt gcgacatatc
     6181 agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga agtttgctta
     6241 attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc tagataatac
     6301 gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt attctcctag
     6361 tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag aagaagcgat
     6421 tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca ataagctatt
     6481 gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga agacaccaga
     6541 agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag tttgcaagcc
     6601 tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc caattaatac
     6661 agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag aatcgggtac
     6721 gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca ttcgaacccc
     6781 ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg caaagattac
     6841 agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta acaaagcaag
     6901 tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc gcaaaaagct
     6961 gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa aaaactttcc
     7021 gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa cttgattgtt
     7081 tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta atagtgttcc
     7141 ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg cgcttactaa
     7201 aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa aaatgaattt
     7261 atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca tgatcaaagc
     7321 agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc taggaactac
     7381 tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg aattactgat
     7441 taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag ttccttggat
     7501 cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca aagaaacgag
     7561 gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca ttgatagttc
     7621 ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct tttacttctt
     7681 cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa gttagcgtat
     7741 ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca cttccaacat
     7801 ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt gcttgtttga
     7861 tgttcatctc taccataaaa cttgttccta atccactttg acatactgca aaaattttca
     7921 tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga tgcgtgtaat
     7981 aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa agcccgaaga
     8041 tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg ggctggatta
     8101 tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc gtttacgcca
     8161 tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa cgcaccatac
     8221 tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt ttctgataat
     8281 agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc cgataattgg
     8341 atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt cttttctaaa
     8401 agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac gccttctttt
     8461 aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat tgaaaatcct
     8521 ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa gtcagtcatg
     8581 attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg tacagtagaa
     8641 aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga attagtctct
     8701 gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa tgaagagcta
     8761 atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc tgcgtccgct
     8821 tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc agggatgctc
     8881 ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta gatggaagca
     8941 tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata ggaaatacga
     9001 aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa cgaagcaaat
     9061 tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc cagcaaaaaa
     9121 tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag agagaaaaat
     9181 ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg tcgactaatg
     9241 tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat ggatgttttt
     9301 ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg atttggtgcg
     9361 aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt ggcgtaataa
     9421 tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag tgactgcatc
     9481 aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc tagcagccta
     9541 aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc atttcttatc
     9601 tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg aaaatgaaaa
     9661 acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct tcgctcttgt
     9721 tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca gtcgattgct
     9781 cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg agttaattgt
     9841 ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag actgatttct
     9901 cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac ctcctactta
     9961 tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta cagttggatt
    10021 ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa gcaggtgacc
    10081 aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat gtttattttt
    10141 tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga tatctttttc
    10201 aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac cagataaagc
    10261 tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt ctttgtcttc
    10321 tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag gggccattga
    10381 acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc ctcctagtag
    10441 ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa acccgattct
    10501 tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct tcttccaaaa
    10561 ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca ttaactaatg
    10621 tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt actttttctt
    10681 catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg ggcaaatcga
    10741 taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct tgtcgatctt
    10801 ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga cgttctttga
    10861 tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa tccagatcga
    10921 agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg atatcatctg
    10981 gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca tataaatagc
    11041 tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa taatcataat
    11101 gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt tggactgtca
    11161 agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct ttttcttttg
    11221 ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc tctactagta
    11281 cattcttacc atgttcgagt gccatttttc catattcata atgaaattga ctaggtgtag
    11341 tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga tcagtatatt
    11401 cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt ttgatattga
    11461 ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc tttccattgc
    11521 caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt cagtatatcc
    11581 tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca gaaaaacact
    11641 tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc aaaaagatag
    11701 tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat gcttattgaa
    11761 gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt cgattacatc
    11821 cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa agctacgtat
    11881 acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa
    11941 ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa tcaaacgatt
    12001 gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa gaaaatggcg
    12061 gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga agatttagat
    12121 caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa aggtgattct
    12181 caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt tttgatcatg
    12241 gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga ggattgcgcc
    12301 atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac ctatttgaag
    12361 catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat ggccaaacag
    12421 gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt agcgactttc
    12481 tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat atattctcaa
    12541 aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca aaaaggcctt
    12601 cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga aagatagtgt
    12661 ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt ttcaaacctc
    12721 agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg aatagctaac
    12781 ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg aaaatcagta
    12841 tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact ttctgtactt
    12901 ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt aagcgatgac
    12961 tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct ttttcgattc
    13021 tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga taagcgagtg
    13081 tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt aagtctgtga
    13141 acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta ttttctcccc
    13201 ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc acagtattcg
    13261 tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata ccgatcgtat
    13321 gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta aagggatctg
    13381 cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg ttttcttgga
    13441 ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga gagatgatct
    13501 gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt atccctattc
    13561 ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt ggtttcattt
    13621 gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc
    13681 tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag
    13741 tacagtccag caatcagtag aaggactact cctgcaccga tatacacata gattccctta
    13801 ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg agaaatcaac
    13861 tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt tgttaatgga
    13921 acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt tagacctgtc
    13981 acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt cgccgatacc
    14041 cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc actatcttga
    14101 tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc accatctact
    14161 ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg atcatttgta
    14221 tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc tttttcgttc
    14281 aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg aatataattc
    14341 ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt tactgggtcg
    14401 atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc attatcaaat
    14461 gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct
    14521 tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat tttttcaccg
    14581 attgcgacat cttttccatt caattgaggt gttgttttat caactttaga cgtatcattt
    14641 ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg cgtgattgtt
    14701 tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt atattttcca
    14761 acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc aaagtcattg
    14821 ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg atcagttgtc
    14881 caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg tgttccttct
    14941 tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt accgattttt
    15001 gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg gatagtatct
    15061 agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc atatacaggg
    15121 aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc tttgatcgtg
    15181 tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt tccatcagca
    15241 tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc ttgttttgct
    15301 gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac gttataaact
    15361 gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc tttcccgctg
    15421 ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat gacttgagct
    15481 gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata
    15541 aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt ttcactcctg
    15601 ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca acaacgccta
    15661 aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt tcttttttat
    15721 tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa
    15781 acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct tttaatcgat
    15841 aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa
    15901 gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg aggtaatttc
    15961 ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt ttatttgtct
    16021 cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta ctgacaccaa
    16081 aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct gtactgctta
    16141 gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac gaagggatcg
    16201 aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct gtcagctttt
    16261 ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca tccgcactat
    16321 ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta tgcaaggaat
    16381 aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag
    16441 gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt gaaagttttt
    16501 gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt tctttgacga
    16561 cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct tgatctttag
    16621 cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt gttgcttctg
    16681 cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg
    16741 ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact tggaatgtga
    16801 caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca tttggatgat
    16861 ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga accatttctg
    16921 tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag aagagacaca
    16981 tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt tcaaaaaaag
    17041 ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta aacggccaat
    17101 tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag aaatctggtt
    17161 gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt gaattgaccc
    17221 atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt ctggtgtaaa
    17281 agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg tatctgtttc
    17341 ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga attttgctcc
    17401 ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg cttttagtcg
    17461 attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc ccgtgtaaat
    17521 gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat catatccggc
    17581 aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa aactggcaag
    17641 acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg cttctttttt
    17701 ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt gcatcgcata
    17761 cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact ggatcgtatc
    17821 atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag cgatcttgat
    17881 ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt ctgtcagcgt
    17941 ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc cgtcgccatg
    18001 atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc caataagatc
    18061 cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga attgtttagt
    18121 atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag gtaattcttt
    18181 gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg ataatatctc
    18241 ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt
    18301 tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag ctgcagcatt
    18361 tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag ctagattgtt
    18421 gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt tagcagatgg
    18481 tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg tcctgccgtt
    18541 catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc tcacttggta
    18601 atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga tggtatttcc
    18661 ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct tgactgaaag
    18721 tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc cattgcttac
    18781 agttgctgag atctgtacag cttttttggc taggtattca gagatatctg ttgcatgatc
    18841 agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc gcatacgaga
    18901 ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga tgccaagacc
    18961 atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg cgataaacgt
    19021 actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc ttgagattag
    19081 agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat aattgctgct
    19141 tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa
    19201 aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat ctcttagtgc
    19261 tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta cttgattttt
    19321 cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc cttcgcttga
    19381 gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta aagtatcgac
    19441 aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca tacttcctga
    19501 ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat ttccacgagc
    19561 attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact ttcgtatcgc
    19621 atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc gatcattagg
    19681 atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat tgccttgatg
    19741 atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag tgccctgatc
    19801 atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag ctggcaagga
    19861 taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg attcttcttc
    19921 tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg tctcatcaat
    19981 aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg
    20041 atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt ccaacgtaat
    20101 tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca catcacttaa
    20161 gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt tattcgcatt
    20221 tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag ctgaaattgt
    20281 ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca caaagtttga
    20341 taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt
    20401 tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt aaaataaaca
    20461 ggcgttacat aattttattt attttaatat gataattatt atacgaaggg cgctaaaggg
    20521 aatcacacca attttcatac caattggttt catttatgaa gttgattcct tcgattaatg
    20581 agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt tgttggaagg
    20641 aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca agtggatgac
    20701 taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa ggtatatcga
    20761 agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa aattggggta
    20821 tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag
    20881 ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa cgattgagta
    20941 ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga aacaatgcaa
    21001 tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc agttaactag
    21061 ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa gttactacgg
    21121 aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa
    21181 taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat
    21241 gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg
    21301 agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct
    21361 cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat
    21421 tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat
    21481 gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat atgttgttca
    21541 attattaacg caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac
    21601 gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg
    21661 atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga tttattattt
    21721 atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga
    21781 taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata ttgaagaata
    21841 tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga
    21901 tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc aacccatttt
    21961 gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa atgaagaaga
    22021 actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa accaccctga
    22081 taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc
    22141 aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga
    22201 gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt
    22261 ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg
    22321 gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca
    22381 agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt
    22441 tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat
    22501 ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat
    22561 atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt
    22621 ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg
    22681 cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta
    22741 tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc
    22801 aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga
    22861 gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact
    22921 tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc
    22981 tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta
    23041 ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt
    23101 gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa
    23161 aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct
    23221 cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc
    23281 ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca
    23341 atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc
    23401 tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt
    23461 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
    23521 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
    23581 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
    23641 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
    23701 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
    23761 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
    23821 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
    23881 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
    23941 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
    24001 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
    24061 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
    24121 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
    24181 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
    24241 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
    24301 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
    24361 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
    24421 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
    24481 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
    24541 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
    24601 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
    24661 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
    24721 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
    24781 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
    24841 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
    24901 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
    24961 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
    25021 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
    25081 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
    25141 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
    25201 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
    25261 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
    25321 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
    25381 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
    25441 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
    25501 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
    25561 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
    25621 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
    25681 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
    25741 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
    25801 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
    25861 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
    25921 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
    25981 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
    26041 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
    26101 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
    26161 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
    26221 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
    26281 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
    26341 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
    26401 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
    26461 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
    26521 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
    26581 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
    26641 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
    26701 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
    26761 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
    26821 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
    26881 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
    26941 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
    27001 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
    27061 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
    27121 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
    27181 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
    27241 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
    27301 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
    27361 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
    27421 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
    27481 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
    27541 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
    27601 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
    27661 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
    27721 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
    27781 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
    27841 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
    27901 tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact
    27961 tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt
    28021 aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa
    28081 tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga
    28141 tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct
    28201 caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat
    28261 ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa
    28321 atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag
    28381 cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta
    28441 aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa
    28501 atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga
    28561 tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact
    28621 attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc
    28681 aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc
    28741 tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga
    28801 gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt
    28861 atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc
    28921 aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat
    28981 taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt
    29041 tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt
    29101 tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta
    29161 gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg
    29221 gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga
    29281 ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga
    29341 tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg
    29401 tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca
    29461 aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg
    29521 cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct
    29581 catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag
    29641 cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa
    29701 ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa
    29761 aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga
    29821 attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt
    29881 cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga
    29941 aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata
    30001 agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc
    30061 ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag
    30121 aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg
    30181 gaacaaaatc aaaacagatt cct
//
LOCUS       DANKHJ010000029        29211 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000029 DANKHJ010000000
VERSION     DANKHJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1743)
                     /locus_tag="PW113_001422"
     CDS             complement(<1..1743)
                     /locus_tag="PW113_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2525743.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNE"
     gene            complement(1853..2422)
                     /locus_tag="PW113_001423"
     CDS             complement(1853..2422)
                     /locus_tag="PW113_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL2525744.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(2440..3855)
                     /locus_tag="PW113_001424"
     CDS             complement(2440..3855)
                     /locus_tag="PW113_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2525745.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(3866..4534)
                     /locus_tag="PW113_001425"
     CDS             complement(3866..4534)
                     /locus_tag="PW113_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2525746.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(4664..5248)
                     /locus_tag="PW113_001426"
     CDS             complement(4664..5248)
                     /locus_tag="PW113_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2525747.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(5579..5812)
                     /locus_tag="PW113_001427"
     CDS             complement(5579..5812)
                     /locus_tag="PW113_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL2525748.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(5827..6777)
                     /locus_tag="PW113_001428"
     CDS             complement(5827..6777)
                     /locus_tag="PW113_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2525749.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(6774..7616)
                     /locus_tag="PW113_001429"
     CDS             complement(6774..7616)
                     /locus_tag="PW113_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2525750.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(7618..8337)
                     /locus_tag="PW113_001430"
     CDS             complement(7618..8337)
                     /locus_tag="PW113_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2525751.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            9029..9187
                     /locus_tag="PW113_001431"
     CDS             9029..9187
                     /locus_tag="PW113_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525752.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            9259..10485
                     /locus_tag="PW113_001432"
     CDS             9259..10485
                     /locus_tag="PW113_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2525753.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(10573..10965)
                     /gene="rpsI"
                     /locus_tag="PW113_001433"
     CDS             complement(10573..10965)
                     /gene="rpsI"
                     /locus_tag="PW113_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2525754.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10981..11424)
                     /gene="rplM"
                     /locus_tag="PW113_001434"
     CDS             complement(10981..11424)
                     /gene="rplM"
                     /locus_tag="PW113_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2525755.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11943..12410)
                     /locus_tag="PW113_001435"
     CDS             complement(11943..12410)
                     /locus_tag="PW113_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2525756.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(12530..14068)
                     /locus_tag="PW113_001436"
     CDS             complement(12530..14068)
                     /locus_tag="PW113_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2525757.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            14148..14834
                     /locus_tag="PW113_001437"
     CDS             14148..14834
                     /locus_tag="PW113_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2525758.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(15035..15562)
                     /gene="msrA"
                     /locus_tag="PW113_001438"
     CDS             complement(15035..15562)
                     /gene="msrA"
                     /locus_tag="PW113_001438"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2525759.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(15646..16323)
                     /gene="pgmB"
                     /locus_tag="PW113_001439"
     CDS             complement(15646..16323)
                     /gene="pgmB"
                     /locus_tag="PW113_001439"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2525760.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(16320..17450)
                     /locus_tag="PW113_001440"
     CDS             complement(16320..17450)
                     /locus_tag="PW113_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2525761.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(17450..19633)
                     /locus_tag="PW113_001441"
     CDS             complement(17450..19633)
                     /locus_tag="PW113_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2525762.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(19630..20655)
                     /locus_tag="PW113_001442"
     CDS             complement(19630..20655)
                     /locus_tag="PW113_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2525763.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(20700..21527)
                     /locus_tag="PW113_001443"
     CDS             complement(20700..21527)
                     /locus_tag="PW113_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2525764.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(21524..22591)
                     /locus_tag="PW113_001444"
     CDS             complement(21524..22591)
                     /locus_tag="PW113_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2525765.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(22617..23453)
                     /locus_tag="PW113_001445"
     CDS             complement(22617..23453)
                     /locus_tag="PW113_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525766.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(23450..24319)
                     /locus_tag="PW113_001446"
     CDS             complement(23450..24319)
                     /locus_tag="PW113_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525767.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(24389..25672)
                     /locus_tag="PW113_001447"
     CDS             complement(24389..25672)
                     /locus_tag="PW113_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2525768.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(25686..27371)
                     /locus_tag="PW113_001448"
     CDS             complement(25686..27371)
                     /locus_tag="PW113_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2525769.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            27646..28671
                     /locus_tag="PW113_001449"
     CDS             27646..28671
                     /locus_tag="PW113_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525770.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         8869 a         5862 c         4794 g         9686 t
ORIGIN      
        1 ttcattgtca agcctccaat catttgcatt ctcataatta gtgaccaatt gtttatacac
       61 ttgatgttta gccattaatt cattatgatt tccacgttct actattttcc cttctgacaa
      121 gacaagaatt tcatctacag atttaatgct ggataatcga tgagctatca taataactgt
      181 tttttctttc atcagatgtt gaaaagcctt ttgtagttca tactcattat ctggatcaat
      241 agatgcactg gcttcatcca taataacaat ctttgcatct tttaaaattg ctctggcaat
      301 agctatacgt tgtttttctc ctcctgacag ataaactcct ttactaccaa ttaaggtatg
      361 ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa
      421 tactttactt gctggcgctt gaggatttgc tatagaaacg ttatcgtaaa tactctgatt
      481 gaatagtttc gtatcttgaa agacaaaagc aattgtgtta ataattgtgt cttttgaata
      541 gtgatttaaa ggcttaccac caattttaat ctgtccctta tccacattgt agaaacctga
      601 aattagttta gctatcgtac tctttccgct tcctgatttt cctactaatg cataaattct
      661 tttttgttct aaaactaagt tgaaattttc taaaacacac tcttctttgt atgaaaagct
      721 gacattttca aatgcaatgt cacaattttt aatctgtgtc tcgttcccat attttagttg
      781 gtcttgcttc atttctaaat aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa
      841 caagtgcata cttacataca ttattctcat gaaagaaaca aaaataattc cacttaaaaa
      901 aaacaacatc aacaagtcaa cagctaatat cttgggagtg gtaagagaag ttataaaaaa
      961 ggttatagga actatcaaaa tgcaaactat gccaaaaaat agccattgaa aaagaacaaa
     1021 aggtgttttc ccactcaaag aatacttata tgcaaactta gcataatcgt taattgcttt
     1081 attcaaggct ttaaatgatg ttactttagc accaaatatc tttataactt gaattccgcg
     1141 gatatattct accgtttcag agcttaacac atctaacgat tcttgatatt tttgaataaa
     1201 atttgtattc tctgtcattt tctttaacaa gaatacacca attactaaca tggcgattag
     1261 aaccaatcca attttaaagt taatgaaaaa gcctaaggca actgataaaa caggtgttac
     1321 tatggcttga gcactatctg gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt
     1381 gtcatcaatt gtctttcgaa tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc
     1441 ttctgttaaa ccgctaattc ccttttttct caaattagtc tctaagcgaa acccaaggat
     1501 atgagaaaat aagccagaaa aaatataaaa taaagctcct aaagttaacc aagcaacagt
     1561 ttgaaccgca taactcttag ctaaatattc atctcctaca ataattagag actgaagaaa
     1621 tttgtaaata ctgtaatatc catagactgt taatacgaca gaaaaatcag aaaataaagt
     1681 ggctaaccat ccgtagtatt tcattttagg gacataacta aataattttt tgtatacttt
     1741 cattttttcc tcccccgcat tcaatttctt ctcttattcg tttacaactg ttaatgcttt
     1801 ccgtaatagc gtaaattaga gatgtacaaa cagataaaag gtaaactacg ttctattttt
     1861 tgtatttttt taagactttt cttccaatca atacaccaat catagataaa cttgctacaa
     1921 ctaacactga aatgattccc catataccta ccgcatagtg gatcattgca tccattgttg
     1981 cagcagattg tcctttatct atccaatctt gacgaaaacg atctgtaaag aaccatatag
     2041 gaatccactg tccggcagca ctgcctaatt gcattagagc ccaagacgca acttgaggaa
     2101 ttgctcgatc atatccaaat acagatagaa taatatcagc taagatcgcc atactaatcg
     2161 aactgataaa ccaaggtaag tacgccccgc ccattaacgt aaaaaataac aataaaacaa
     2221 ttgtgtaaat agtaaaaatt ccttttctag ggtagttata gctaatataa acaaatatga
     2281 ttccagataa taatccaaaa atcccggggg atatgctgtg accaaacgcc ccaccaatac
     2341 tactaaccat tattgaaaaa aagtaaatta ctagggaaat aactgtagct aaaccaataa
     2401 aaatataatc ttttgttttc atatatattc tcctcaattc tatttcgtta tgtcattaaa
     2461 aatctgttct atcaaagttg aatcttctac tctaagtttt atatcttttt ttattccacc
     2521 attttttaaa taaataattc tattgcatgt tttataaaga aattccggat catgagtagc
     2581 tataactact gcaccctttt cttttaagtc tttaataagg tcagcaatcc tttccatatt
     2641 aatataatcc aacccacttg taggttcatc aaatagaaat aaattagttt ttgacaatga
     2701 agctatagaa accaataatc tttgtttttc tccaccggat aaatcaaacg gtaattgatc
     2761 tttcatttta taaagttcca ttttttttaa aattttttct tgctgatctt tactaactat
     2821 ttcattcccc agggctaact cgctcctcac tgattctgtg aaaaattggt aatccatttc
     2881 ttgcattact aaaaacggcg atgacaaatt ttttttattt tcgattatct ttcctttggt
     2941 agggcgaata atcttcatga gtattcttag taaggttgtt ttccccacac cgtttttccc
     3001 taccaaacca ataacatctc caactttgac atcaaaattt atgtctttta gaatattttc
     3061 ataagtaatt tcttcaactt ttaatacatc tgcacttacc ttttcgatat agggaacttc
     3121 aatcgaacta ttaaaaatgt caaatttcct taatgcgtta ttgttttcag tttttaacaa
     3181 ctgaatagtt gtagttttta cagttctatt tttaacaaat agaacttgat caattaattc
     3241 tttcaaatag taaaaacgat gttcagctat aacaatagta aatcctttat tttttaaatc
     3301 acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc aaatttgaag acggttcatc
     3361 caatataatt aatttagagt ttagcgataa actacatcca atagaaattt tctgccgttc
     3421 accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta ataattcagc
     3481 taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa
     3541 tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt gacttacgta
     3601 gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat tttcaaataa
     3661 aatttcccca accaattttc cgtcataaag tcttggaata agtccattca ttaactttaa
     3721 tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactgc ctttttttat
     3781 tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt
     3841 gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg
     3901 aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa cttcttttca
     3961 caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca atcatagttg
     4021 cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga aaaaaagaca
     4081 agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa cgataagtcg
     4141 gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa tgtatttttt
     4201 ctaaagagga taataattca ttgctagtat agacactaac caacaaccat gagatcatta
     4261 cgaccggcaa cattcgaaat acaatatata aggatgtcgc aaaaaatata gtaaaaaact
     4321 ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa atcaagatga
     4381 aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac
     4441 accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta
     4501 aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg
     4561 cactgttatc ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca
     4621 aaaagcataa ctgagaacaa ttctcaatgc aagctttttt taattaactc agtaattcaa
     4681 tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc ttttcttttg
     4741 aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga
     4801 tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt aacttatcta
     4861 gacaacagac tgtctttttc gtcacaaatg atgtaatttt ttcaacaata tcttgatatt
     4921 tggttccagc tgattggaat aaaatcaaat cgaatatacg tttattctca aaaatataat
     4981 taataaatgg atctaattca ttgaagttct ctctccaact tttctctata tctatatttt
     5041 ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc gcattaatat
     5101 gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta taaaaagcac
     5161 ccgtagttac gcctgcggtc aggcacagtt gccttatctt agtttgctga aatccttctt
     5221 tttcaaaatg gcttagtcca ctttctatta acttttggtg agttataata taatctctgg
     5281 tttctatggg tacttcctcc taataagatg aattaacttt attatcttac acaggacttg
     5341 aagtttcaat cgttaacgtt tattactgaa ctcattcctt agagctaaac taatgagaaa
     5401 gagcagaata actgatacaa aaagcaatcc tacaagaatt aatagactca tctaaatcat
     5461 tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc tattaggata
     5521 agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat aaagtctttt
     5581 acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc
     5641 ctatatctaa atagtcctca tatctaagtt tagccagcat ctcacttaca gatgctggac
     5701 taacacttag aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt
     5761 cctgaattgc tagtaaataa attagtatag tagtcgtttt aatttgtacc atacctaccc
     5821 tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata
     5881 gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat
     5941 aggaatattg gtatcttttg atactgtttt cattggtcta tcatccacac tgctttctac
     6001 gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg
     6061 tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt
     6121 cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga
     6181 agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag
     6241 attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt
     6301 tttagcgtaa ataataccgt tttctaaatt taaccaagca tgaggatctt ccttcccttt
     6361 ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt
     6421 gttttcctct ttgttcgcat ttttaactaa ttttgtaaac caagcatttc ctccagtttc
     6481 caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg
     6541 caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt
     6601 atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta ctacttttaa
     6661 tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg ccccgaatat
     6721 aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac
     6781 gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat
     6841 aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaata
     6901 aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat catagttttc
     6961 aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc aacaattaat
     7021 atagttccca cactttgcat agctgttact gctactaaag ttaatagaac catcaataga
     7081 taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc aaaagaagtg
     7141 attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga aacgccaatt
     7201 gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat atgaaataaa
     7261 tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa agaactgaaa
     7321 gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg
     7381 atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa
     7441 gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct
     7501 cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt
     7561 agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta
     7621 ttccccctct ccaataaata tagtatctcc ataggctttt tttaaatttt cttcattaaa
     7681 tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa tattatcaaa
     7741 atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc cctgatgttt
     7801 caattttttt aacgtatcca taattattcg ttcactaact aagtctatcc ctacaaatgg
     7861 ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa gtacacgttg
     7921 aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt
     7981 ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat
     8041 tccggcatat gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat
     8101 atcagctttt tgctctacat atgcaatctt caccagtttt ttattgatat cttcattatt
     8161 aatggagacc tctccctgat gagggataat atttaacatt gctttaagca atgtggattt
     8221 tccagcacca tttggaccaa taatacctgt taaagcacct tcttgtattt ttaaagaaat
     8281 ctcgttcaat gctaaaaaat cgttataaca aacattaagc tttttcactt ctaacataac
     8341 tttctccttc taaatgataa tgattatcac gatcatatgg tatacaaaaa ttatatttta
     8401 gtcaacccta aattttatag tttagatttt tttctgttgc tgaagattag aatgacaaaa
     8461 ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata tataaaaaag gagcgttctc
     8521 aaatgagaac atgctcctaa aactacttta ttggcgtatg gaaaaattat acttgaagtg
     8581 gatagcgggt tgaataagtt gataagcatc aaaacattta taaaaattat tcaagactat
     8641 gtctgttcca tatacaaata tgaaaatcag taaactccat ttaacttttt tcgtagtgtg
     8701 tctttttata catgtttatc gttattaata gtgaatagga aaaaaactat ttttgatcat
     8761 tgacaattaa atacaatttt tgaataacga tccctgctat tcgagctaga aactcaagta
     8821 cgccatgacc tgattagcga caaatactta gagatttctt tttgttctgg gaaaacaaaa
     8881 tgccatgaca atagcagcaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga
     8941 ggtgaaattc ctatgtgctt acccaaagca attcaaaaat ttcataacta ctattttagg
     9001 agatgatttt atagaatcca gagtctacct gaataaaaaa caaacctgtg catttctagg
     9061 aatcgataat aatactctag ataagtggat tcgtatatat aatttcccag taataagagt
     9121 tggtggtgta gttcgctttg acgtagaaga tattaaagac tttatgaata catttaaaat
     9181 aacttgaaat tttcttctcg ccgcatatac taaaattagt gaaatatact gccttacttt
     9241 ttttaaggag tgaggatttt gcctacaaag ctttcaaatg ggaaatataa aactaatctg
     9301 agatatccaa aaaggtttaa agaaataaca ggaattgcat cagaaaagta tcaaaaaaca
     9361 tttccaaata ggcaattggc gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa
     9421 gttttgagag aggagaatgc aaactcatta gaattaaaag gaaaaataac gtttaaaaaa
     9481 ttttacgaat ctaaatggct tcctagatat gaattaggtc aaacaattag atcaaacagg
     9541 cctcctagtg atataaccat tagtaataca aaagatatat ttcggttgca tattttacct
     9601 atgtttggtg aatacgcaat gaattatcta aatatcaata ctgagataat atcagatgaa
     9661 ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca taaaaggata tgttcgttcc
     9721 atgttcgata ttgcagaaat actaaattat atcgaattta atcgaactac taaaattatt
     9781 caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa aaagaataca agaaggtaag
     9841 caagcactat catcaaaaga acttactaat tggatagaag cagtaaatga tgattttaat
     9901 aatcatttac ttacttttca tgattacaca ctttttatga ttaccctgta tcttggtgat
     9961 agaaaaagtg agacttacgc tttgcaatgg aaatatattg attttgaaaa acaaacggtc
    10021 cgtcttaaac atactttaga taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat
    10081 accgtcattc aagtaccaga agttgtaatg acattgctaa gtgaatggaa atcggtacaa
    10141 gctgatcaat tgttgaaatt aaaaattaag caaacgttag atcagtatct ctttacttat
    10201 actaaaccat ctggagaagt aaattgtcct gttcatgccg attacctaaa ctatcgaatc
    10261 aattcaatta aaagaaggca tcctgactta gttcatttat ctccacacaa actccgtcat
    10321 acttatgcaa ctatagctag acaaggtgga gcagatatga atcaaatttc aaatgcttta
    10381 acacactcag atatttctac aacaaagata tatgtgaaca caccagatat agttgataag
    10441 gctgtttttg aagcttttca gagaggattg aataagtgtg attgatatgt gttcttaaat
    10501 gtgttcttaa aaacaaaaga agcactttcc aaataattga aaagtgccag aaacgttgat
    10561 atataaagga tattaacgtt ttgagaattg tgaagcttta cgtgctttct taagacctgg
    10621 ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa
    10681 acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga
    10741 ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc
    10801 agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc
    10861 aacatctttt ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac
    10921 tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta
    10981 ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc
    11041 tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat
    11101 accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt
    11161 gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt
    11221 tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa
    11281 atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag
    11341 acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg
    11401 tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca
    11461 tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg
    11521 ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact
    11581 tcttttcttt cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc
    11641 tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc
    11701 ctcggtcaca agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt
    11761 gtgactcgga gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga
    11821 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc
    11881 taatactcag gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt
    11941 ttttacgcgt cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt
    12001 tctgtggctg ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca
    12061 gaaacttcga ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt
    12121 tccaaacgtt ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta
    12181 tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat
    12241 acttcatcta actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta
    12301 aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt
    12361 agatctgcaa ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc
    12421 tcctaacatt ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat
    12481 catttacctg agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag
    12541 aatcagctaa ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt
    12601 gtgtcgaaac aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc
    12661 caacttgctc tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga
    12721 tgcttctcca gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa
    12781 caagcgggta acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc
    12841 cgcctgtagc acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat
    12901 aaaggttgag acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc
    12961 gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa
    13021 gtaaataggg atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa
    13081 gcagtgcatc atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa
    13141 atgtatgccc tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc
    13201 aatgtttctg atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag
    13261 tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa
    13321 aagcacctaa ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat
    13381 catttggcaa acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg
    13441 aaggaaagac ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg
    13501 cccattggaa ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga
    13561 tcagttcgcc aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt
    13621 ttttgaataa ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg
    13681 gatgaatcgg cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt
    13741 tttctgcttc gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt
    13801 tctcttcatc caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga
    13861 ttgtgtctga aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga
    13921 tcaattcctg atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat
    13981 aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc
    14041 cgatatctaa tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa
    14101 atttagttta agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt
    14161 ttatcattgg ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag
    14221 gtcactctat tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt
    14281 atgaattgat tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt
    14341 gtcggttgcc cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt
    14401 tctttacttt ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc
    14461 tattttcgtt gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt
    14521 gggcaatttt atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt
    14581 taacgtttat cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg
    14641 atactctgtt gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct
    14701 tgtttcttgc tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa
    14761 aaaagttgga aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc
    14821 atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg
    14881 ttctcttacc aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa
    14941 atggtgttca atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga
    15001 tatacggtgt tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac
    15061 gaaggaaacc tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct
    15121 tgtaataatc ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg
    15181 tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa
    15241 tatttcgttc ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag
    15301 cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat
    15361 cataagaaat aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc
    15421 ctgttgttac ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca
    15481 cagaaatgat ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg
    15541 cgaaaactgc tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc
    15601 aaaaggagga taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac
    15661 ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac
    15721 atattcagca gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc
    15781 caaagctgct tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac
    15841 attcaattgc tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg
    15901 cgtgccgtca ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg
    15961 tgcatttttg cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag
    16021 aacagctatt gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata
    16081 tagattgttt ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat
    16141 ttttccaaat gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc
    16201 gttttctttc tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta
    16261 atgataacga gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt
    16321 catcgattac ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg
    16381 cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg
    16441 atcggaaaaa cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt
    16501 ccgcaaatag ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct
    16561 tttcctttta gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc
    16621 tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc
    16681 cctgaagcaa atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc
    16741 tcgattactt gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct
    16801 aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg
    16861 cataaaagat tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc
    16921 acgttcgttt tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt
    16981 tgattatctt gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg
    17041 ttggtactac ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt
    17101 cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg
    17161 acatctttca atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg
    17221 tactggattt ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac
    17281 cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga
    17341 tcatattcag cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct
    17401 aaggctgtca gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc
    17461 tccgttccaa gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta
    17521 atgtttcttt cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc
    17581 tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag
    17641 aaatagaaag agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc
    17701 atagtgctcc taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta
    17761 aatcaatcag gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc
    17821 aagcctctat ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat
    17881 agtaatgcaa attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca
    17941 gcatgacaag atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat
    18001 caagtaaaat agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct
    18061 tactgagata cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata
    18121 acttatctaa aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa
    18181 cagctaaggc cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt
    18241 ctgtgtattc atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag
    18301 ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa
    18361 acgattgatc ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga
    18421 tatgatgttc tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg
    18481 cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac
    18541 cttgatagtg ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca
    18601 gcagcaaatt ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt
    18661 ccgtatccca gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac
    18721 taacacttgg atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg
    18781 ctaactgatc gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg
    18841 tccaataaga catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg
    18901 caatactttt cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct
    18961 ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat
    19021 tttttgcttg gaatacacct gctttattga atttagctgc aatcccaata tcaattttac
    19081 tttctgttgt ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt
    19141 caaccaactg ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta
    19201 cttggcagtc ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat
    19261 tttgatcagc aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga
    19321 gcgcatagcg tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt
    19381 tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg
    19441 ggagattcac taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa
    19501 acattcccct tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc
    19561 catttccaat ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt
    19621 cttctacttt cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac
    19681 cagattctgg atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag
    19741 cgcccgattc acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt
    19801 ttgtaaggaa accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc
    19861 acctgcctga tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa
    19921 cgaagtattc agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc
    19981 ttctactgtg aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc
    20041 aattttttgg aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat
    20101 cgctgtatca agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt
    20161 gtcgataaac gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta
    20221 ataaatatcc ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg
    20281 atgctggaat ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct
    20341 tgcttgatca gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag
    20401 ggcagccaat acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg
    20461 agaaatctca ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg
    20521 gcaaaagtct tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc
    20581 tgatcttttt tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat
    20641 tgctactttc ttcatttatt tctccctcct ttgcttattt acttattgtt attctttctc
    20701 tattctttct ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc
    20761 aatgattcct gataaatttt cacttcattt tctcctcgca aacgacattc atataccata
    20821 taaccttcat actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca
    20881 gatcctggtt ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc
    20941 gtagtcaatg cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg
    21001 atctgcacat gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg
    21061 atcatatggt cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg
    21121 gctactttat ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt
    21181 cggggtgaaa ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg
    21241 attcctttgc ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca
    21301 ttaagacgcc gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc
    21361 actactggga tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac
    21421 aacttaccgt cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct
    21481 aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc
    21541 ggacttgaac gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag
    21601 cttctgcgct ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac
    21661 cgttcatcaa taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat
    21721 ctgggtttgg ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt
    21781 cgcgaccaag attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc
    21841 cgccatagcc gatccctctt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa
    21901 taactgaatc tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt
    21961 tcataggatc tagcgtgtaa tcggcaccgt atttttctgc taattctcta cgctgcgcta
    22021 tgggatcgat cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt
    22081 gcccgatagc gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc
    22141 ggataccacc taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca
    22201 accgatcaag tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa
    22261 tgggtccata agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt
    22321 cttcgatcgt accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt
    22381 ggcttccttc ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa
    22441 atggacttaa tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta
    22501 ctcggatacg tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta
    22561 ttcttggttc taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat
    22621 ccttttacgc cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata
    22681 atgatgactg gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct
    22741 ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg
    22801 aaaatcgaag caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa
    22861 atgattccag aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga
    22921 ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc
    22981 atgaatatcg cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta
    23041 tcatacaaac ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa
    23101 aacatataaa cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt
    23161 gacaaagcgt atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag
    23221 atcactaaac tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc
    23281 tgataatgtt caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct
    23341 tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat
    23401 agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt
    23461 ccaccttctt tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata
    23521 tagctgctgc ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta
    23581 agatattgac tttattcgtc agtagataga catctgtaaa catatagaac atccagatag
    23641 atcgtaaaac aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga
    23701 acttctgcca agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg
    23761 tttgcaaaat agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca
    23821 aaacgaccat aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa
    23881 gctgcaacct atctactaac agatcattga cgattccata actattattg aacatatatt
    23941 tccaagcaaa aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca
    24001 ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg
    24061 tactgcctgc tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt
    24121 cactatcact taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag
    24181 gattcagcgg catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta
    24241 aaacagctaa taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat
    24301 gtttccttgg acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt
    24361 tttcgaacac cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag
    24421 cttgatcgac atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg
    24481 agcttgtgat atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta
    24541 cttcatcaaa agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat
    24601 tttctttgta accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag
    24661 acatcaagac ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag
    24721 cagcctcttt ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat
    24781 agacagcttg atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt
    24841 cataaacaga aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga
    24901 aagcatcttt gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt
    24961 gataaaacga caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat
    25021 ccaaaacatt tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc
    25081 cttcagcagt aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag
    25141 caatttttaa aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt
    25201 ctttgtcata ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc
    25261 catcttctga acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct
    25321 cgataacgtt ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa
    25381 aatcctgact gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt
    25441 ttgtattgaa cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt
    25501 tttcaaaatc agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt
    25561 gcatgaattg aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag
    25621 taagaagaaa tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc
    25681 acctcttatt ttattatttt cagccacata tattcatacg gctggaatat caactttcgt
    25741 tcttgactgc aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa
    25801 agataacctg ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct
    25861 tcgcgaataa aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga
    25921 tggaaggctt tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct
    25981 gttcttaatg gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga
    26041 ttgatttttc gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc
    26101 acactttgaa tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct
    26161 aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac
    26221 gtagcgttta tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg
    26281 gtaccctctt tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct
    26341 cgtaccggat tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt
    26401 ttcgatgcga aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga
    26461 atcgcttgaa gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg
    26521 ccattcccaa aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc
    26581 gttcctggag ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct
    26641 ggcaggtgta tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga
    26701 ataaatgaag cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact
    26761 tctggatttt taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt
    26821 tctttcccgt ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg
    26881 ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag
    26941 tttaaatatt cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac
    27001 attaaccgtg tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg
    27061 attgcctgat aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag
    27121 aaatgtacaa ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc
    27181 aaagttggtt gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct
    27241 tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt
    27301 tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt
    27361 tttgtataca tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt
    27421 atggaaaacg cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac
    27481 aaataagaaa gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg
    27541 atcatgcaag ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact
    27601 atggaattat tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt
    27661 tatgacattg cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac
    27721 cagccaaata tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat
    27781 tatgaaccga gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca
    27841 ttatctcatc gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa
    27901 tccatttctg ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca
    27961 ccagaagaag agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt
    28021 attattttaa gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt
    28081 cctattgtgg taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg
    28141 gataatttcc aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat
    28201 atcggctgtc tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac
    28261 cgtcaatgct tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc
    28321 tttcgcttag aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca
    28381 actgcgattt tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa
    28441 tctggaaagc agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc
    28501 tccctatttt cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa
    28561 gccacagaac ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt
    28621 ctttcaaccg agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt
    28681 ataaaagaac agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat
    28741 ccctcgttac cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat
    28801 agctgtggat aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta
    28861 tttttaacca cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta
    28921 tttcaatcca tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg
    28981 tctactggtt ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat
    29041 aaccgctcta tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt
    29101 gttgattgac ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg
    29161 ttgttaagat cctttcccac ttttcttcta gttagacaaa ttattttaga c
//
LOCUS       DANKHJ010000030        27807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000030 DANKHJ010000000
VERSION     DANKHJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            628..1797
                     /locus_tag="PW113_001450"
     CDS             628..1797
                     /locus_tag="PW113_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2525771.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            2270..2824
                     /locus_tag="PW113_001451"
     CDS             2270..2824
                     /locus_tag="PW113_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2525772.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2997..3470)
                     /locus_tag="PW113_001452"
     CDS             complement(2997..3470)
                     /locus_tag="PW113_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2525773.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3482..4018
                     /locus_tag="PW113_001453"
     CDS             3482..4018
                     /locus_tag="PW113_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2525774.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            4058..4429
                     /locus_tag="PW113_001454"
     CDS             4058..4429
                     /locus_tag="PW113_001454"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2525775.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            4713..5984
                     /locus_tag="PW113_001455"
     CDS             4713..5984
                     /locus_tag="PW113_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2525776.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            6229..7254
                     /locus_tag="PW113_001456"
     CDS             6229..7254
                     /locus_tag="PW113_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525777.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7548..8657
                     /locus_tag="PW113_001457"
     CDS             7548..8657
                     /locus_tag="PW113_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2525778.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            8740..9636
                     /locus_tag="PW113_001458"
                     /pseudo
     CDS             8740..9636
                     /locus_tag="PW113_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            9652..10476
                     /locus_tag="PW113_001459"
     CDS             9652..10476
                     /locus_tag="PW113_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525779.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10508..11818
                     /locus_tag="PW113_001460"
     CDS             10508..11818
                     /locus_tag="PW113_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL2525780.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11966..13225
                     /locus_tag="PW113_001461"
     CDS             11966..13225
                     /locus_tag="PW113_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2525781.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            13235..14107
                     /locus_tag="PW113_001462"
     CDS             13235..14107
                     /locus_tag="PW113_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525782.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            14119..14949
                     /locus_tag="PW113_001463"
     CDS             14119..14949
                     /locus_tag="PW113_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525783.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14989..17172
                     /locus_tag="PW113_001464"
     CDS             14989..17172
                     /locus_tag="PW113_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2525784.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            17233..18453
                     /locus_tag="PW113_001465"
     CDS             17233..18453
                     /locus_tag="PW113_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL2525785.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18450..19910
                     /gene="gtfA"
                     /locus_tag="PW113_001466"
     CDS             18450..19910
                     /gene="gtfA"
                     /locus_tag="PW113_001466"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL2525786.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19987..21093
                     /locus_tag="PW113_001467"
                     /pseudo
     CDS             19987..21093
                     /locus_tag="PW113_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21415..21624
                     /locus_tag="PW113_001468"
     CDS             21415..21624
                     /locus_tag="PW113_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2525787.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21617..21766
                     /locus_tag="PW113_001469"
     CDS             21617..21766
                     /locus_tag="PW113_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL2525788.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21920..22585
                     /locus_tag="PW113_001470"
     CDS             21920..22585
                     /locus_tag="PW113_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2525789.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22869..23954)
                     /gene="xerS"
                     /locus_tag="PW113_001471"
     CDS             complement(22869..23954)
                     /gene="xerS"
                     /locus_tag="PW113_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2525790.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            24288..24521
                     /locus_tag="PW113_001472"
     CDS             24288..24521
                     /locus_tag="PW113_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2525791.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24518..24925
                     /locus_tag="PW113_001473"
     CDS             24518..24925
                     /locus_tag="PW113_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2525792.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26062..26580)
                     /locus_tag="PW113_001474"
     CDS             complement(26062..26580)
                     /locus_tag="PW113_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2525793.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27698..>27807
                     /locus_tag="PW113_001475"
     CDS             27698..>27807
                     /locus_tag="PW113_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2525794.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         9551 a         4212 c         5113 g         8931 t
ORIGIN      
        1 atactagtat gatatatatc tatagcaaac agttaaaatc ttcctaaaat aagcgaatag
       61 cattcctaaa acctataaaa tttttagatg aagcgacaag tggacaatta ataaactact
      121 tgaacatgaa attatggatc acctttcaaa tttagaaaat ggaattctta tacttattgc
      181 gcaacattta ttgattgcaa aattatgtga caaaaaaaac tagtagaaga aagaataatg
      241 gcttgataat aaagtactac ctattaaagg tagtacttta tttaatttag tataaattaa
      301 catactacta tattttaggt ttcatattat ataaattaat aatatactac catttgttgg
      361 tagtataatt taaaggtaat aagttattct tttattacta tgtttgggtt ttatagcaaa
      421 gaaagtttaa taagagtttg gacctagcaa acaatattaa tgaggtcatt aaatcttatt
      481 atctttagtg atataacgaa atatctacac agacaaaatg atagcgtaaa atattttgtg
      541 taaatagata tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca
      601 accgaaattt tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca
      661 ctaatcaata aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat
      721 tcattattgc aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg
      781 ttcaattcag gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga
      841 gaattaaatt tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct
      901 gcttataaaa gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga
      961 attactatgt ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctatacgcca
     1021 caaaccattt caaatatgac taaaatagtt tctgaagata ttattgcctt taaagaaagg
     1081 tccttagaat ccagatactc tgtcattttt atggatgcta ctcacattcc tttaaaaaga
     1141 caaactgttt caaaagaagc agtttatatt gtaattggca ttcgattaga tggaaccaaa
     1201 gaggttcttg ggtttagtct tgctccaact gaatctgctt atgtttggaa agagattctt
     1261 caagacctaa aagaccgtgg tttaaaagag gttttattag tcgtaactga tggtttaagt
     1321 ggcattaacg atagtattca tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc
     1381 catatatcta gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga aatctgtagc
     1441 gatttcaaat tagtttatca agcaagttca aaagaagaag caaataatca aataaggttt
     1501 atgatagata aatggaaaaa gcagtatcca cgagtggtta aattgctcat gaatcctgct
     1561 atattaacct tttataattt ccctccgtca atcagaagaa ctatttattc aactaatttg
     1621 attgaaggtt ttaacaaaca attaaagaaa tataccaaaa gaaaagagca atttcctaat
     1681 gaagaatctc tggagagatt tctagtatct caattcaaca actataatca aaagtttcta
     1741 tgccgagttc ataaaggttt taaagaaata catgatacat tagaatcaat gttttaactt
     1801 gatcaacaga atgggctttt ccatttacac aaaattattg acgctcccga caaaattatg
     1861 atcttgcgta aagttataaa atattaataa aaatatttta ttaatgaaaa gcttggtagc
     1921 acggttattc atatgctaat ttatttcact ttatagtaag gggagttctt agtatattgt
     1981 tcaaacttaa atttcatata atttaattat gtaaattagc tattctaaga acttctacgg
     2041 ataactgccc gtctaaaatt taaataagag cttagaatct tcatataagt aaggttaatt
     2101 tttttgtaca aaaaagtcgg aatattctga gttactgaaa tgatataaaa tcataaacta
     2161 aatcccttga ttttattgac tttcaaaagt ttatatatat aagttaatga ttatggtaaa
     2221 atatatacat aatcattaac tcagaatatt tttaagaagg gaaacaatag tgagctataa
     2281 tgtacaaccg ttaaggacaa atgaagagat acaagatttt cagttttggc taagaagaac
     2341 aagtaatccg gaaagagata gttttttatt tttgtttgga atcaataatg ggttacgtat
     2401 gtcagatatt ataggattaa aagttggaga tatacgaggc aaaagtaagc ctattattgt
     2461 tgaacataaa acaggtaagc gaaaaccgat ttttatagat aatttgagag aagaaatttt
     2521 attatatact gaaggtaaag aagaaaatga ttggttattt ccgagtcggc aacaaggtcg
     2581 acatataaca agggatcgtg tctatcagat atatgcagat atagcagaaa aattaggacg
     2641 agatgatata ggaacacata cattaagaaa aacttttggg tatcactact acaagaaaac
     2701 tagagacatt gctaccttaa tgtttatttt taatcatagc tcgcaagcaa ttacaaaaag
     2761 gtacattggt ataactgaag atgaaatagg agcatcactt cgtggattta aactaggtgt
     2821 ataatttaaa aatttttata aacagtaaag acaaagatta aaagctattt ttatctagaa
     2881 cgattttatt tcataaagtt cgcggaataa aagctggggt tactggtact tggtataggt
     2941 tcgtgttata taaactaagt aattaaataa catttttagg ttggaaaagg atgaaattaa
     3001 aaaaagagaa tcaatacggt ctcaaaaacg caaacgctgc ggaataagaa aaatactgat
     3061 aactcaacgt ttagcagact atctaatagg gatagtctgc ttttttcgtt tttgaccata
     3121 cttttgatct tctgcaaact tccttgcttt tcctctgtag aactcatgaa atctgcaaaa
     3181 cgaattgccg tttcctctgt ctgctttttc gcgatatggg tgtatatatc atccatagtg
     3241 gttttaatat ctgtatgtcc aagtcgaaca tacacttctt tgacagacgc accagattca
     3301 aagagcagac tacaatgggt atgacgaaaa ccatgaagtg tgatatgttt taagttattc
     3361 tctttaatga tgattttcaa attgtgattt acatagtcta aataaagggg cttattttct
     3421 tctgatgaaa acaaaagttg ttcaccatct tttataggtt tgattctcat ttttagatat
     3481 tatgcccgca cgtattcatc acatttctat tgtgaatcgc tttattcgac cgacatttga
     3541 cttttatcat aatatactcg gtttaaaatt actaatgaaa acaattaatc aagacgatca
     3601 taccatgtat cacctgtttt tttcagataa tcaccaccgt gtgggaactg aactgacttt
     3661 ttttgaagta caagaaggga acaaccagtt ttttggaacc aatacgattg aacgaacgat
     3721 actaaaagtt ccttccgaag catcattgca tttttgggaa atatattttg aaacacaagg
     3781 cgtttgccat tatggaatcg aatcattttc tggacgacct atcttacgtt ttgaagggcc
     3841 agatgcaaca cgattagctt tagttccctt acgcgaattt gaagatattg atgattattt
     3901 cccgtatgtc acagaccaga tccctacaga gcatgccatt ttaggaattg atgccattca
     3961 actacgcgta caatattccg atgcgactga acgagagtta ttgtcggtag gctggtagca
     4021 caaagaatac gtcagtttct tttcgggtca tcaacgagtg acaatcttag aaaatcaaga
     4081 cactcagttc tatcaagaag ttcagattat tcaagatcgt gagaatccag ttgccattga
     4141 agggattggt agcgtccatc atgttgcgtt tggcgtagaa aataaatctg atttacaacg
     4201 catcgacaaa caattacaag agcgcaattt tataaattca ggaataaaag atcgtgaatt
     4261 ttttgtatca ctttattatc gtgacccaaa tcaattattg attgaaattg ctacagggga
     4321 aggaaaccta gatgcaaaag cgtatgaaaa tcaaagtcca cgctttgaag aaattccttt
     4381 atttttaccg cagtacttaa atttcattcg tgaacaagtg gagcaataat tagctaatca
     4441 aatccatgac gaagagtaag aggttgactc ataagtcaaa aaataaagaa aaacgcatca
     4501 aatccattct aaagaatgtt gatttgatgc ggttcctatt tttataaact atcattttcg
     4561 actaataaaa ctacttatga gtcagcctct ctttttttct tgatttttat acctctttct
     4621 aggtaaacta aacatctgcc aaaaataccg tgtctcattg aaacattctc aactatttca
     4681 aaataaaggg gagtaccaaa tgaacgagta tattgaaatt atccacgcaa gagaaacaac
     4741 ttaaaaatgt cagtctcaaa attcccaaaa ataaaattag tatttttacc agcgtatctg
     4801 gttcaggaaa atcatcgatt gtttttgata ctattaccca agaagctggt cgtcaactga
     4861 atgacaccta tgatagtttt acccgtttat ttttacctaa atacagtcgt ccagaggttg
     4921 atgagattga taatctttct acggcgattg tggtcaatca aaagaagatt ggtggcagtg
     4981 cgcgctcaac gttagggacg attactaaca tcaatgcgtt atttcgtgtg ttgttttcat
     5041 gttttgcaac acctagttta ggatatgcaa atgcttattc atttaatgat cctagtggaa
     5101 tgtgttctgt ctgtcaggga atcggtaaga cgattgcatt tttaatagga ttgctttatt
     5161 tttcggctat tgataatgct gattttgtat tttctgatcc atttaccgca gaagttttgt
     5221 ctggaattga ggaacaggca aagaatcatg gttattttgt attggtgcat aatgttaatt
     5281 cagcagaaga tgttaaaact attcagagaa attggcgttt tgaggggttc attgttgttg
     5341 gtgtacgtgt aaaagaattt ttggaattaa ataacgtttt tgatggtcct gttgtttata
     5401 ttgatacgca tctttcaaaa gagacattaa gagaacttga acaacaaatg aatcgtttat
     5461 ttgtaaatac aaatgattac caagctagta cattggcagt ggaacatttg attcattatg
     5521 gtcatgagaa aatagcgttt ctttcttttg actacattgt aggtgaacca agtgttattc
     5581 aagaacgaca tcaagcttat atcgatacat taaagaaata tcatttaacc tttaaatcgc
     5641 attggatata taacaactct gaatttgagc gaatttatca agaattagct gaatttactg
     5701 caattgttgt tactggagat tatttagcgg ctaaattagt aaaattttta aaaaataaga
     5761 ataaacgagt gatggaacaa ttatcgatta ttggatttga tgatattact tttgcagaat
     5821 taatggatcc tgcgttgaca acaatacgac taaatccagg taacaagggg gcgattgctt
     5881 ttcaccaact gattggactg gccaaacaag aagaaataaa aaatcaagta ctactattag
     5941 agggagaatt gatttctcga gactctgttc acaaaatcaa ataaaaaagc tctgtgaaat
     6001 aagtatagtt aacaataaaa tatccgaacg atagagagaa tgaactgtga tctagcagtt
     6061 cctcgtcgca aattcactag aattagcatt atttactcta aattgttcgg atattttttc
     6121 ttgtttttaa ttatgattac agtgcctatt tttaacgaaa tttgcttatt ttcaaaatgt
     6181 atgattatat gttagtaaac actttcaaat atgaaaagag gagctattat ggtaggaatt
     6241 agggatgttg caaaacgggc gggtgtgtcc attgcaacag tatcgcgcgt gctaaatgag
     6301 gatccgacac tttcagtaac cgatgaaacc aaacgaaaga ttgcagaatc ggttaatttt
     6361 tatcattatg tcaaaaaaag tcccgttgca aaaaatttaa caaatattgc attaataacg
     6421 acagtttctg aagtagatga gttggaagat ccctattttc gaacgattcg cagagggatt
     6481 caagtcgaag cagaacaaag caaagtcaat ttgaagaaaa ttattcgttt gtcagaaaca
     6541 ccgttggagc aagacgattt gctaaaatgt caaggtgtgt tgattattgg gcaggtgcta
     6601 tcttctgtga ttgaagaagt atcagcaatt aattcgaatg tcgtagtgat tgatgaccct
     6661 agtattgaag ctgaagtaga tgcagtttat actgatttaa caaaagcaac gaatcagcat
     6721 ttagatcgat tgtataaaaa aggacaccga aacattgcgt ttatcggagg aggacgtgtt
     6781 cgtttagata gccaaggaaa taaacacgtg tcagatgatg attatcgaaa aatagcctat
     6841 gagacgtgga tgaaacaaaa aggattagaa aaatatagtc aaatttattt agaaggctgg
     6901 accacacttg acggaatgca ggcagcagat ttacttttga aaaaacatgc taaagataag
     6961 ttgccgactg ctatcatggt aggaaatgat cctattgctg tgggggttta tcgttcactt
     7021 caaaaaaatg gtctacatat tccagaagat atttcgattg tgagttttga taatattgaa
     7081 gttgctgaat ttttaacacc tgcattaaat acggtgaatg tgaatacaga agaaattgga
     7141 aaactagcag ttcgcatggt taatgaacga ataaaagaag ttagaaatgt ttcgatcaaa
     7201 gtgattgttt ctaataatat tattgttaga gaaagtgaaa aaaatatcga ttaaaaaagt
     7261 aaacatccaa aaaagggtgt ttactttttt gtttactaat atttttagta aaaaatctcg
     7321 acaaaacatt aagtaaacag ttatggtgtt gttaacaaaa agaaaacgca ttcaaaacag
     7381 gaggaattaa gatgaaacat ttcaaaaaaa tagctttaac agggatagct gttttaactg
     7441 ggacattact cgcagcatgt ggtgggggag atagtaattc taaagacggg caagtaactt
     7501 taagctatgc gagttgagat actgaccaag ctccaggact aagaaaaatg ttagatgaat
     7561 ttgaaaaaca aaatcctgat atcaaagtag aaatggagac aacgccttgg gatcaatatt
     7621 gggttaaatt agaagctgca acaacgggag gtaatatgcc agatgtagta accatgcact
     7681 ctagcgaatc ctataaatat atgtcacaga atgctttgat gggacttgat gatgttatca
     7741 aagaaaatga tattgacatg ggaaacttta ctgaaggaat tgctgatttc tatacgcttg
     7801 aagacgaact atacgcaatc cctaaagatg cttctgtagt ggggctatgg tacaacaaag
     7861 aattatttga taatgcaggt gtcgattatc cagatgaaac atggacatgg gaaacactca
     7921 gacaagctgc aattgattta acagatgaat caaaaggcat ctatggtttt gcatcacata
     7981 ttggaacaga gactggctac tggccattta tttatcaaaa tgggggagaa gtatttgccg
     8041 atgacaatac aaaatcaggt ttgaataccc cagaagcaca agaagcttta gctttttatc
     8101 gagatattgc acttgttgat catgcttcac caacaattac agaattacaa gatcctgata
     8161 agttaagtcg ttttcaatca ggtagattag caatgatgat tgaaggaaac tggtttgcac
     8221 cgggattttt gaaaaatgac tatctagttg aaaatgttgc ttctgccgtg ttacctaagg
     8281 gaaaagttaa tgcaacaatt acaaatggac ttggttgggc tgccagtgcg agtacaaaat
     8341 acccagaaga agtgaaaaaa ctaatggcat ttttagctag tgaggacgcc aataaaatcc
     8401 aagcggaaac gggggcatca atcccagcac taaaaggctt tgaagacgag tgggctgctt
     8461 cttcagatac aattgatttg agtgcttttg gaaaaatgtt ggagtatgga gtccctcgtc
     8521 catacaataa agatggatta cgagctgaac aagcagaagt tcaaattatt aaccgtattc
     8581 tgtctggtga agtaactgtt gaagatggaa ccgctcaaat tgaaaaagaa gtcaatgcga
     8641 tattgagcgg aaattaaatt agttaattat acaatataca ggcgaaagga ttctatttcg
     8701 tctgtatatt aatggatgaa taccaaaaag ggggatatta tgaaaaatcg aatttttgga
     8761 aaatatcgta aagatcaagt gatatggggc tatattttta tttttccaac ggcattagga
     8821 ttaattttat taaatatatg gccagcaatt caaacagcaa ttttaagttt tcaaaaaacg
     8881 gtagggttcg gtgatactga atgggtcgga ttaagaaatt accaaaaaat gtttgcagac
     8941 ggtgaagttt ttcagtcttt attaaacaca ttaatttatg cagtggtttc gatacctagt
     9001 attgttgtat tatccttgtt ggtggcagtt ttaatgaata gaaaagttaa aggattgtcg
     9061 atttatcgga caatttattt tctaccagtc gttgctgcac catccgcagt cgcaatgatt
     9121 tggcgctgga tgttcaataa tgattatgga ctgattaata acatgttggc taaaataggt
     9181 ctcgatggac cagcatggtt aacggatcca tcgattgcga tttattctat tatcattgta
     9241 ggagtttgga gtgctattgg ttataacatg gtgttattac tagccggctt ataagaaatt
     9301 cctaaagatt attatgaagc agcggaaatt gatggtgcta gtccaatcca gcagtttttc
     9361 aacattacgt taccattagt tacaccaaca ttattttttg tagttgtaac aacagttatc
     9421 aatgctttcc aagtatttga tgttattttt atgatgatta ctcctaatag taccgccatg
     9481 tttaaatcac aatcattagc ctatttattc tataaacatt catttatttt aaatgacaag
     9541 ggatatggtt cggcaatcgt tatgttcctt ttagtgatta tattaattat tactgcaatt
     9601 caaatgaagt tgcagaaaaa gtgggtaaac tattaaggag gctatcaaat tatgaaaaat
     9661 aaattttttt caaatgcgcc aatccatatc atgcttattt taggtggtat tgcaatggca
     9721 ttacctttta tttggatgat tttaacttca gggaaaacgt tggttgaatc tacccaaatt
     9781 ccacctacaa ttttccctga gaaatttcag ctaagtaact atcaacaagt acttgattta
     9841 ttaccattca tggcttttta tatcaataca tttttaatgg ttgttggacg agtgattggc
     9901 tcggttttat tttcagcaat ggctgcatat gcttgtgcgc gtctaagttt tccagggaaa
     9961 aatatccttt tcagcttagt acttattcaa atgatgattc ccggttccat ttttattact
    10021 ccacagtatt tattagcaca aaaattgggt attttaaata caattccagc attaatatta
    10081 ccaggggtag ttagtgcgtt tggtacgttt ttattaagac aatttttctt aggtttacct
    10141 gatgaattgg aggaagcggc ctatttagaa ggtgccaata tttggacgat ctttttcaaa
    10201 attatgttgc cgcttgctaa atcaggattg gttgcacttg gaatattcac agcgttgttt
    10261 gcatttaaag atttaatgtg gccattgatt gtcaatactt caattgataa gatgccttta
    10321 gctccgggat tagcaaactt acaaggacaa tatgccacga acttcccaca attaatggca
    10381 ggttcgttat tagcaatttg gccaatagtt gtgatattca tcgttttcca aagaaaattc
    10441 attgaaggaa ttgctacaac aggtagtaaa ttataaaact attaattagt gattggagag
    10501 taagtacatg acaaaaatta catttattgg tgcaggaagt actatttttg caaaaaacgt
    10561 attaggcgat gcgatgttaa ctccaagtct acaagatgca catattgcat tgtacgatat
    10621 tgatgaaaaa cgactaaaag aatcaaattt aatgcttaaa aatattaata aaaattcgaa
    10681 tgaaaatcgt gctgatattg tttcttatta tgatcgcaaa gaagcgttaa gagaggctga
    10741 ttttgttatc aatgctattc aagtgggtgg ttataaacct tcaacagtga ttgattttga
    10801 tattcctaaa aaatatgggt tgcgacaaac tattggtgac accatgggga ttggtgggat
    10861 ttttagaacc ctacggacac taccagtaat gttagacttt gctaaagaaa tggaagaagt
    10921 ttgtccaaat gcttggttat tgaactatac aaatccgatg gcgatgttaa ccatgggtgt
    10981 tttaaaagcg acgagtatta agactgttgg tttatgtcat agcgttcaag tatgcgtacc
    11041 agaattgttt gagcatttag gaatagccga tcaatatgac ttaaatgact ttcaatggaa
    11101 aattgcaggc attaatcata tggcattctt gttggaaatt acaaaaaatg gggaagattt
    11161 ctatccaaca attcgtcagt tagctgcaga aaaaccaaat ccacatccag attctgtacg
    11221 ttttgaattg ttgaaacgtt ttggttacta tattacagaa tcaagtgaac acaatgcaga
    11281 ataccatcca tactttatta aatcaaaata cccagaattg attgacgaat tagggattcc
    11341 gttggatgaa tacttacgtc gttgtgaaca acaaattaat gattgggaaa gcatgcgaga
    11401 ggacattgta aataatgggg agttaacaca tacgagaagc cgtgaatatg cttcatatat
    11461 catggattct attacgacag gaactccgtc agtgattgca ggaaatgtct taaataaagg
    11521 cttaatcaca aacttaccga cagatgtttg tgtggaagta ccttgtttag tcgataaaaa
    11581 tggcgtgcaa ccaacctatg tcggtgaatt gccaacacaa ttagctgctt taaatcggac
    11641 gaatatcaat gttcaagaac tgacagtaga agccgcttta acactagata aatcaaaaat
    11701 ttatcaagca gcgtatttag atccacatct atcatctgaa ttgtctttaa cagatatcaa
    11761 aaatttagtg gatgatttaa tcgaagcaca tggggactat ttacctgaat ataattaaaa
    11821 agaagctgaa agagtaagga attttcaatt ccttactctt ttcttagtta aatttagtaa
    11881 aaatattgac aaaacactta gtaaacagtt acattagtgt taacaaaaag aaaacgcatt
    11941 caaattgtat aaggaggagc ataaaatgaa gtggtctaaa aaaattattt tagcttgtat
    12001 agcactagga gcaacgatct ctttgactgc ttgtggaaaa aatcaggaag atgcaagcgg
    12061 taaggtagaa atagagtttt ttaatcaaaa aaaagaaatg actcaaacga ttcaggagat
    12121 tgctaaagat tttgaagcaa aaaaccctga tattcatgta aaagttgtgg atgtaccgaa
    12181 tgctggtgaa gtaattaaaa cacgtatgct tgcaggcgat gtaccagatg tcattaatct
    12241 ttatcctcaa agtatagaat tgaaagaatg ggcgaaagct ggttatttag aggacttaac
    12301 cgaagaaccg tatttagaga atatcaaaaa tggatatgca caacgttttg ctattgagga
    12361 tcgtgtatat agcataccat taaccgctaa tgtctatggt ttttactata acaaaactgc
    12421 ttttgaggaa atggggattc aagcaccaga aacttgggca gaatttgaaa aaatagtagc
    12481 tgagattaaa gatcaaaaca aagtaccgtt tgcgattgca ggagcagaag gctggacttt
    12541 aaatggttat caccaactag ctatagcgac tgctgctggt ggtgaagagg aagcgaacaa
    12601 tgtttggcgt ttctcagaag ttaatggaat taatgctgaa agcaaagaaa tgcaaaaaga
    12661 ttttaatcga ttagatttgt taagagaacc aaaagctttg caaaacaact ggcaaggtgc
    12721 cggttataac gatacagtag ttacttttac aaaaggtgat gcgttgatta tgccgaatgg
    12781 ttcatgggca atgccgatga ttcattcaca aaatccagat tttgaagtag gaacttttcc
    12841 ttttccagca gatgaagcag gtcaaagtct aacgattggc gcaggagacc tagccttatc
    12901 agtttctgca acttctcaac ataaagaaga agccaaacgt tttgtagaat atatgacaac
    12961 acctgaagcg atgcagaaat attatgatgt agatggtgca ccgtgtgctg ttgagggcgt
    13021 tatcgagaat actcaggatt caccattaag tggtttaact gaacttgctt ttactgatcg
    13081 tcatttagtt tggttagcca aagattggaa tagtgaaaat gatttttata ccttaacaac
    13141 aaattattta cacaatggca atcaaaaagc catgattaac gcattaaatg ctttctttaa
    13201 tccaatgaaa gctgacggaa ggtaggaaaa taaaatgaaa ataaaagaaa ttttaaatcg
    13261 ttactgggca atcatttttg tagcaattcc aattattttg cagatgattt ttttctactt
    13321 tccaatggtt caaggtgctt tttacagttt aactaattgg actggtctga cttataattt
    13381 tgattttgtg ggtttgaaca attataagat tcttttttct gatccgaagt ttatcaaatc
    13441 gattggcttc actttaattg tgacgctttg catgattgct ggacaaattg gttttggtat
    13501 tttgattgcg cgggcgttga acgcaaatat taaagggaaa acattcttta gagcatggtt
    13561 cttttttcca gcggtcttat cgggcgtggt tgtttcttta gtatttaaac aaatttttaa
    13621 ctatggttta ccaaaaattg gtagtatgtt aggcattgaa tttttacaaa ccagtctttt
    13681 aggaacaaca tggggagcga tttttgcagt aatttttgtt ttactttggc aaggaattgc
    13741 catgccagtc atcattttct tagcgggttt tcaaagtatt ccacaagaaa ttattgaagc
    13801 tgctagaatg gatggggcga atgataagca aattttttgg aatattgaaa tcacctactt
    13861 attaccaagt atttcaatgg tatttattat ggcattaaaa ggtggcttaa cagcatttga
    13921 tcaaattttt gctttaactg gtggtggacc aagcgatgcc acaacatctt tggggttact
    13981 tgtttataat tatgctttta aaaacaatca gtttggttat gcgaatgcta ttgctttagt
    14041 cttatttatc ttaattggga ttgtttcact ggcacaatta aaattatcga aaaaatttga
    14101 aatctaggag gtttcccaat gaaaacatca aaagaaaaaa atatttctaa atacttgctt
    14161 ttattattag ggggcttcat gattttagtt cccttagttt caacgctatt cggttcattt
    14221 aaaacaacac ccgatattat gaataatttc tttcgttttc ctaatccagc gactttggaa
    14281 aatttccgaa gattgttagc ggatggaatt ggtcattact tttggaattc ggcaatcatc
    14341 acaatcattg cagtagttgc tgtgacagca attattccta tggctgctta ttcactagca
    14401 agaaaaatga ctcgtgggaa agcatacgga ttgatgtatt tatttttaat tttaggaatt
    14461 tttgttcctt tccaagtaat tatgattccg attaccgtta tgatgagtaa aattggttta
    14521 gcaaatatgt ggggattaat cttactctat ctcacttatg ccgtaccaca gacactattt
    14581 ttatatgttg gttttattaa actaaatgtg cctatcagtc tggatgaggc tgctgaaatt
    14641 gatggggcta gtcactttac aacttatttc aaaattgttt tcccaatgtt aaaaccaatg
    14701 catgcgacga ctattattct taatgctcta tggttttgga atgatttcat gctaccatta
    14761 ttaattttaa atcgtgattc taatatgtgg acactaccat tgtttcaata taattatacg
    14821 ggacaatatt ttaacgacta tggtccaagt tttgcttcct atacagtagg aattgtcacg
    14881 attacactag tttatcttgc tttccaaaga agtattatag ctggaatgag caatggagca
    14941 gtgaaataac caataaatta ttctcattat aaaggagttc ataaacttat gattatattt
    15001 gacaatcaca cacaaacatt tcatttacaa aataaaaaaa ttagctatct attaggaatc
    15061 gaagaaaata attatttaac gcatctttat tttggaaaaa agattacgca ctattctggt
    15121 gggtatcgct acccccgaac agatcgttcc ttctcaccca acaatgcaaa tgcgcaagat
    15181 cgtttattat ctttagacac attaccatta gaatttccag gatatggaca tggtgatttt
    15241 agagaaccgg cgcataatat taaattagga aacggtagtc gtgtaaatga ttttcgttat
    15301 gatagctacg aaattcaaaa aggaaagccc agcttagcgg gattgccagc cttatatacg
    15361 gaagatgatg atgaagctga aacgctgatt attcaattga aagacgcgat tagtggattg
    15421 actttacagt tgttctacac aatttatcaa aatcacagtg taattattcg ttctacgcaa
    15481 ttagtaaata atagtgatga aacagtggag attaaccgct tagctagtca atcaattgat
    15541 tttccaaatc gtccactcga gttaattcat ttaaatggta cctgggcaag agaacgtcaa
    15601 atgactagag aaaaaattca tacagggata aaagtattag atagtaaacg cggatcaagc
    15661 agccatcaac aaaatccgtt tgttgcttta gtagatgcca aaacagatga atttcaagga
    15721 gaggcatatg gatttaattt agtttatagc ggtaatcacg aaacggtgat tcaaaaagat
    15781 ccatttgacc aaacacgggt cattatggga attaattcat ttaattttgg ttggcaacta
    15841 aaaactggcg actcatttca aacgccagaa gtcgtgatgg tttatagtaa tgaaggtctg
    15901 aatacgttat cacagacgta tcatgaattg tataatcacc gtttaattcg tggaaactat
    15961 cgtttaaaag aacgtcccac gttaatcaac aactgggaag caacgtattt tgattttaac
    16021 gaagaaaaaa tcatgaatat tgtcgatgat gcgaagaact tgggcattga aatgtttgtt
    16081 ttagatgatg gttggtttgg cggtcgtgaa aatgacttca cttcattagg tgattgggtt
    16141 gaaacagaag gcaaacttgc ttgtggtttg gaaaatttag ccaagcaagt gcatgataac
    16201 gggatgaaat ttgggatttg gtttgaaccg gaaatgattt ctgaagatag tgaactattt
    16261 cgagcacatc cagattgggc attgggtatt ccaggtcgtg accgctcaat tagtcgaaat
    16321 caatatgttc ttgatttttc aagacaagat gttcgtgata atatctatca acaaatgacg
    16381 gcgatttttg atcgtgtacc gattgattat gtgaagtggg atatgaatcg aaatatgaca
    16441 gaagtctatt ctgtcttgtt ggactcaacg atgcaaggtg aagtggctca tcgttatatg
    16501 ttagggttat atgaattttt agaaaagtta acaacgaatt atccaactat tttatttgaa
    16561 agttgttcgg gtggaggcgg tcgttttgat gcaggtttct tgtattacat gccacaaact
    16621 tggacgagcg acaataccga tgccgtggct cgtctgaaaa ttcaatatgg aaccagctta
    16681 gtgtatccaa tttcaagtat gggtgcgcat gtttcagcag tcccaaatca tcaaacagga
    16741 agagaaacga gtttaactat tcgtggcaat gctgcgatgg ctggtgtttt tggttatgag
    16801 ctagatctaa cgcaattaac ttctgaagaa aaagcagaaa ttaaagaaca agttgccttt
    16861 tataaaaagc atcgccaact tttgcaatat ggaacttttt atcgcttgga aagtccgttt
    16921 gaaggcaatg atactgcatg gatgtttgtc tcaaaagacc aaacagaagc catggtcttt
    16981 tatttccgag tattagcaga agctgcgcat ccattagtca cattgaaatt agcaggatta
    17041 gcagaagaag caacttatca aatggaagat gtaacatttg ctggcgatga attgatgaat
    17101 ctcggttttt atattgactc agaactacat ggtgattatg cgacacaacg cttttatttg
    17161 aaacagatat agtagcttga atttacaata taagtgcaac accaaaagag actcattcca
    17221 aacgaagaaa atatgtttta tttacacgca tttactaaaa agcagcctga tttgaattgg
    17281 gaaaatgaag aagtacgtga agaaatctat gcaatgatta actattggtt agataaagga
    17341 ctaggcggat ttagaatcga tgcgatatta aacatcaaaa aaaaactaga gtatggcatc
    17401 tttgaatctg atggagaaga tgggttagcc ttcattgggc cctggattct taatcagtcg
    17461 ggcattgaag tgtggttaaa agaaatgaga gagcggacat ttaaaccaca taatagtatg
    17521 acggtagcag aagccgatgt tcctaatgaa cgtttagatg aatatattgg tgaagatggc
    17581 tattattcga tggtttttga ttttagttat acggatattg atgtacctga aactggtgaa
    17641 tggtttaaag atagccaatg gacatggacg gatatgcgta ccaatatttt tacgaatcaa
    17701 ttagttacgc aagataaagg ttggggcgcc ttatatttag aaaaccacga tcaaccacgt
    17761 tcaattaata aatatattcc agaagaatgg atcaatgatt atagtaaaaa aatgttggcg
    17821 acgcttttta tgttactaag aggtacccct tttatttatc aaggacaaga actaggaatg
    17881 accaacatag aaatggcatc attagaagat tttgatgatg tagctacgca ttctcagtat
    17941 aaacgggcat tagaatatgg attggcacca gagcaagcat tgaaagctgt caataaacga
    18001 agtcgggata attctcgcac acctatgcaa tggaatacgc aaaaaaatgc cagattctca
    18061 acttctgata atgtatggtt aaaagtaaat cctaattatc cagaactcaa tgcagaagct
    18121 cagttaacca atcaacagtc ggtattcaat tattatcatc aattaattaa attaagaaaa
    18181 aatagtgtat acaaagacgt gctaatctac ggacaatttc taccagtgaa agataaaaat
    18241 gaacatattt tgctttatga acgccaatta gaagataaaa tcgtattagt tatcttaaac
    18301 gttacagcaa cagaacaaga atattatgta gatgaacagt accaacaagt attaatcaat
    18361 aattatgaag atgttttatt agatcaaagt cgaaaattgc gtttacgacc gtttgaaagt
    18421 attgtattag ctaattatgg aggacaatta tgaaaattaa aaacgaagca atgctcatca
    18481 cctatgcaga tagtttagga aaaaacatgg gcgaattaaa acaagtctta gacaaacatt
    18541 tacaaggtgt tattggtggt gtacatctgt tgcctttttt tccatcgaca ggtgatcgag
    18601 gctttgcgcc gagtgattat acgacagttg atccagctct tggaacatgg gaagatgtgg
    18661 aagcattagg tgaagattat tatttgatgt ttgattttat gattaatcac atttctcgtg
    18721 aatcaaaatt tttccaagat ttcaaaaaaa accatgagca gtcaccatac aaagaaatgt
    18781 ttatccgtat tcatgaattt ttcccagaaa atcgaccaac acaagaagat attgatttaa
    18841 tctacaagcg taaagacaaa gcgccgtttc aaacggtaga atttgcggat ggtacgacag
    18901 aagaggtttg gaataccttt ggggaagaac aaattgattt agatgtgacc aaagaagttg
    18961 tgaaagaatt tattcgagaa accatcaaag atatggcgaa ccatggctgt tctttaattc
    19021 gattagatgc gtttgcttat gcgattaaaa aattagatac caatgatttc ttcgtggaac
    19081 cagatatttg ggaactttta gacgaagtgc gtcaagaagc agcgaaatat gacgtcgaat
    19141 tattaccaga aatccatgag cattattcca ttcaaatgaa aattgccaat catgattatt
    19201 atatctatga cttcgccttg ccaatggtca ctctgtattc actttatagt ggaaaatcag
    19261 aacgtttagc caattggttg aaaatgagtc cgatgaagca gtttacaaca ttggatacgc
    19321 atgatggaat tggtgttgtg gacgctcgtg atttattaac agatgaagaa ttggattaca
    19381 cctctgagga attgtataaa gtcggggcga atgtgaagaa ggtctattca agtgccaatt
    19441 acaataattt agatatttat caaattaaca gcacctatta tagtgcatta ggcgataatg
    19501 accgtagcta tctgttagca cgtgcgattc aagtatttgc gccagggatt ccacaaattt
    19561 attacgttgg gttattagca ggaaaaaatg atattgaact gttggaagca acgaaagaag
    19621 ggcgtaatat taatcgtcat tactatgatt tagccgaaat tgaagaagaa gtgcaacggc
    19681 cagtgattca acaattattt gcacttttaa aatttagaaa tgaatctgcg gcgtttgatt
    19741 tggatggaac gattgaagta gcaacgccat cagaatcaga aatagtgatt acacgtaaaa
    19801 ataaaaacgg tagtgatagt gcaacattaa cggctaactt ggatacgaaa gaatttatca
    19861 ttgagtctaa agatataaaa attttcacta gtgttgcatt aaaattttaa cctattccat
    19921 agaacaaaaa atcctttata ctagcacttg gatgacttat ccaagaccaa taaaaaggac
    19981 tcatacatgg ataagtataa aacaaataca gcatttaata aatggttttc tgccataaaa
    20041 ttaggaaaac tttctgtcaa ctcccaaaaa tgtatttttg attacaattc gtatcgtaaa
    20101 aaactgtctt ttgaagctgt gctcaagctc tttctctacg cagttaatga agaaaaagaa
    20161 agtttgcgtg atttgagtac agctcttatc gaccgatcgc tccaactaga aaccgatgtg
    20221 tcatcgatta gtcataccca attgtctcga gcatttaatg cacttgaacc taaagtgtta
    20281 gaagaaattt ttcaacaact gttagaaaaa gttcaacatc aaatcaaacc aaccaagcga
    20341 aatcatctgt atctgattga ttccagtacg ttttcgctta gtttaaaacg tcataagtgg
    20401 gcgactttcc gtcaaacaaa atcaggtgtc aagttacact taaactattg ttatatggac
    20461 gatactacga tgtatccaac tgattttacc ttaacaaatg cccatgaaca tgatctcaat
    20521 caactaccag tattggtgaa tcaaccagag gcaacttacg tgtttgatcg tgggtattag
    20581 gactttgaaa agatggatca gatgcattgg gatggctatt tcttcgtcac tcggattaaa
    20641 aaaaacacca aagttcacgt gatagatact ctggaaacat cgccagaaac agaaattcta
    20701 cgagacgagc tggttcgttt aggatctaaa acctatctaa ccgccaattt ccgactagtc
    20761 actgttcaag ataagaacgg acgtgtgttt caatttatca ccaatcgaat ggacgtctct
    20821 tcaaaagaaa tctctgatat gtatcacgct cgttggcaaa tcgaattgtt tttcaagcac
    20881 atcaaacaac acatgaccat taaaaccttc ttttctcaaa gtgaaaaagg cgtgcagaat
    20941 caattgattt taacgatgat ttccgctctc ctcacgtttc tcatcaaatt ggagacgaaa
    21001 acggaaaaat cagtgttcca aatcaaacga ttctttcgtt atttactgtt tcagcccttt
    21061 gaatgttgtc tagaaaaatt aattcccaca tgagaaaaaa gaaatttggc tcgttacttt
    21121 tggttttcag ttgaataaca gataaaaaat tttctggaaa aagtcatcct ctgcgtcagc
    21181 acagtttttt ttgacttatt ttcagaggaa atagttttca gaatattcta aaatgtttta
    21241 tgcaacacta gtgatatttt ttagaattag atttctaatg tgcatttttt gagtattaag
    21301 gagcaagtat acaaagattt ataaagctga ttaaatttga ataaaaatat gcaggaacgc
    21361 tactattttt agtagtgttc ttttttatat ttgaataaaa aatgtggtgg ttaaatggtg
    21421 gttataacaa aaaatgaatt aatgaaatta ggttatggga agtatcagtc tgagagaatc
    21481 atacgacagg caaaagcaat tatggcttca aaaagctata cctattacga aaatagaaga
    21541 ttaggactag tacctgttga atctgtagaa gaagttttag gtatgaagat agaggtagag
    21601 gaggattttc agaatcatgg ttaattttaa aaaagataaa aatgggaaat atcattttac
    21661 tgctagtttg ggattcgaca aacgaacagg gaaaagagta caaaaaaacg tagaggggtt
    21721 aaaacagtta aagaagaaaa aaagcgtata ccgaacttgt ttctaattat ggaaaagaag
    21781 cagaaacttc tcaaagtact atgttgtttg aggagttttt ttataaatta ttcctacctt
    21841 attatcaagg gaaagtagaa gaaagaacat ataaaaatcg gttatctaca atcaaaaaaa
    21901 cttttcttat ttctttaaaa tggagttaag agaaatacgt ccaattgaca ttcaaaagtg
    21961 gcaaaataat ttattgagca aatataaaaa ttcttatgta agaaacatgt atgggatttt
    22021 tcagatgtca ttagatcgag cagttgtttt gggactactt gaaaaaaatc ctgcaaaagt
    22081 tattggaaat gtgaagaaaa ggaaaactac agttgatttt tggacaaaag aagaatttac
    22141 aaaagttatt tcaacttttt atattgatga tttgtaccaa cattttggtt ttatttgtat
    22201 gtggttactt tttatgaccg ttatgcgttt gggagaagcc actgcattaa cttggaaaga
    22261 tattgatttt gaaaataaaa acttgcgtgt gaataaaaca ctctattata agagagccaa
    22321 aacctatgag tttgtagaac ccgaaacggc ttctagtatt cgtacaatcg aactagacga
    22381 caatacaatt acctttttaa aagcttggaa ggagcgtcaa gtttcaatag gaggcatgtc
    22441 gtttgtactt tcttataatg gaacacctac tcagaaatat acaatccgtc atattatgag
    22501 aagacatgca acgatcgttg gtgttcatat aatccgcata cacggattaa ggcatagtca
    22561 tgcttctttg cttatatcac aataaaaaga tgcacttcga attaaagaaa ggttaaaaca
    22621 tgctgatgta caaacgactt taggaactta tggtcatttg tatctaaatt cattgagaga
    22681 gataacaaat gatcccacag attatatcca aactaatttt tctaaaatca aaccgacacc
    22741 tatttacgat aataaaaaat aaatgataaa caaaagtagg atcacaccaa aagaaacaca
    22801 ctagaaaaac tataatacag gcttttctag cgtgttgttt cttatttccg cctatttata
    22861 cattatactc ataaagcatt taatgcatcg atcgtagccg cattgtctat atgggtatat
    22921 aaagttgttg tattggtact tgaatgtccc aattgttggg cagttaatac caatgaattt
    22981 gtctgttggt agagtctagt agctaatgta tgacgcagtt tgtgagggct gacttgaatt
    23041 ctaaagcctt ttgaatattt tgctaccatc cgttcgattg caataccgga tatacgcttg
    23101 gcttctcctt ggtaaattgc tagaaataaa gctttttcgt tttctgttac tttatatcgt
    23161 tctttccgaa tagctagata attagctaaa tattccattg cgaatgctgc tatattgaca
    23221 gcatctcttt tatttccttt tctagttact accacattat tcttatctaa agataggtca
    23281 tctaaattaa tatttataac ctcggagagt ctcaatccag atcctaaaat cagtgcattg
    23341 atcgccaggt ctctttctac atctcgttga taatagatca aagctcgttt actcaaagta
    23401 tttttatatt cattggcaat ataatctaga taattttgtg tatccttatc tagaaataat
    23461 tttttctcaa tggcattggc acgtgcagct aacgtagttt tatctgtgat ggttgctact
    23521 tgatccatag gattattgtc taagtatggt tttcctagag gattattgga acttttcgaa
    23581 agatatttaa atagaacctt taatgccgtg atatttctat tgatagtaga agtagataga
    23641 cctaagttat tattatcttt gagcttagtt tttgttgtct ctttttttac attttctttt
    23701 gtgcgacgtt tcaaatgaga tttgtacaag tttaggtcat aggcagttaa ctgtcttatc
    23761 gaggtgatag aagtatcagg aacgatagac tcactcagaa gccattcata aaagagctta
    23821 taatttttta gatattctaa agtggttcga ggtgattgat ctttatcctc acgatcttga
    23881 acaaaatctt ttatataatc gggcatatga agtatgattg tatctatttt ttgatatatt
    23941 tctgtatttt tcatgtcatt catcctttca ataagtaggg gagaagagta tctctataat
    24001 tggactatac caattataga atgttttgtc aattggtatg ctctaaaatt tattaatttg
    24061 tttcaaaaaa agcatataaa caggtatacg tttaataatt taaaataaat ggtatacata
    24121 aatagggaag gaaatagaag ggagcaatct aaattttttg aattttctta attaattata
    24181 tagaaagaac aatctttcta tagagttaga catgagaggg actatgcttg atttaaaatc
    24241 aacaagcgta tatactgtat atacaaagaa aaaggaggga gaagactatg gcaattaaag
    24301 aaagaaaatt tagggaagtg gggaactcag tagtattaac tatttcaaag gagtttctta
    24361 aagaaaatgg gttaaatccc ggagatacgg tttttttaga tgaagaaaaa cttcgagcgg
    24421 caattacaaa aaaacaagcc ttatctgatg aaattgattt agcaatatct caagcattaa
    24481 ctgaatatga tactactttt agagagcttg tcgataaatg agtattcatt acctagaagc
    24541 caaagatatt ataaaaatga atgtaatcca aataaaaaaa tactctccta atgaacccct
    24601 tggtgtaaag gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag cctcagcttt
    24661 tggtgaagag gtttatcctt cgatttatga aaaagcagct atcctactta tacaattgat
    24721 aaagaaacat ccttttcaca atgcaaataa acgtactgct ttcttagcta cttttgtctt
    24781 tttgaaaatc aatggacagc ttttagctat tgatcaaaaa gatgctgtaa atttagcggt
    24841 atatattgct acgtatgata aggatttcga tcaactaaaa tatgaagtga tcaacactat
    24901 aaagacacat acgcagccca tttaaggagt tactgtatgt gtctttatag ggttattagc
    24961 tgctatagcg aaataattga agtaaattac aaatagctat aattcaaatc caactatata
    25021 gaaaacgtaa attttctata tagttggatt aagataacga ctatttattt tatcatattt
    25081 ttactatacc ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata
    25141 ctactagttt gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata
    25201 cacctacaac ttataaattg atggtattta gataacacga aagaataaaa ataacagctg
    25261 atagtaaagt ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg
    25321 cttctgaaaa cttatgaaat gaaagacctt ttacatcttg cagaaatcgt tactgggatt
    25381 aaactaagca ataaaaacta atgttcccct cattctttga acttctacta tttgatttat
    25441 ttattctgat aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt
    25501 aagatctctt cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag
    25561 aataaactgc tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc
    25621 caaattataa accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa
    25681 gtcagaagat ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata
    25741 tttaaaggga attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc
    25801 tttcccctgc tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta
    25861 tcgaagtgag tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac
    25921 cctctacggc attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca
    25981 aagaacttat gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat
    26041 tgcttagcag taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct
    26101 aaaagtccca aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc
    26161 cgttttggtt gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact
    26221 cattgaatca atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc
    26281 tttcaatggt agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt
    26341 ccatttaaat tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca
    26401 ttttagaatt aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc
    26461 cacttcctca actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata
    26521 tttagtataa gtcctattta tttctttttt taattcttct ttatttttat atgttctcat
    26581 tatattacct ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga
    26641 tattttaatc cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga
    26701 atgatttata aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct
    26761 aaaaaatacg aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt
    26821 cgtaaatatc aaaatcatgt taactactat caattactaa taaaaactac cactttaagc
    26881 gttaagaact gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc
    26941 acgaaagtgg taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat
    27001 tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct
    27061 catcagatga aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa
    27121 aagcgattgc catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa
    27181 gatcatcctt tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca
    27241 atgatgtttt caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa
    27301 aagaaaattc accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt
    27361 ttttccaaaa ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata
    27421 acttcttttt aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg
    27481 atttttatga ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag
    27541 tatggacgtt tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt
    27601 agtgaactac ccccacttag cttggatagg ctacaattaa ctagacagaa aaattaaggt
    27661 gtgtagacta gaagaaaaca taccaggagg aatttttatg tctaagagaa cacgaagaac
    27721 tttttcacaa gaattcaagc aacaaatcgt caatctttac ttagctggaa agccacgtgt
    27781 agaaatcatt cgagaatatg aactaac
//
LOCUS       DANKHJ010000031        27561 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000031 DANKHJ010000000
VERSION     DANKHJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            131..505
                     /locus_tag="PW113_001476"
     CDS             131..505
                     /locus_tag="PW113_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2525795.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PW113_001477"
     CDS             complement(619..1248)
                     /locus_tag="PW113_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2525796.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PW113_001478"
     tRNA            complement(1382..1454)
                     /locus_tag="PW113_001478"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PW113_001479"
     tRNA            1668..1741
                     /locus_tag="PW113_001479"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PW113_001480"
     CDS             complement(1879..2043)
                     /locus_tag="PW113_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525797.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PW113_001481"
     CDS             2338..3666
                     /locus_tag="PW113_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2525798.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PW113_001482"
     CDS             3868..4701
                     /locus_tag="PW113_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2525799.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="PW113_001483"
     CDS             4717..5454
                     /locus_tag="PW113_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2525800.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="PW113_001484"
     CDS             complement(5609..6577)
                     /locus_tag="PW113_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2525801.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="PW113_001485"
     CDS             6809..7642
                     /locus_tag="PW113_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2525802.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="PW113_001486"
     CDS             7676..8515
                     /locus_tag="PW113_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2525803.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="PW113_001487"
     CDS             8541..9101
                     /locus_tag="PW113_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2525804.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="PW113_001488"
     CDS             9300..11021
                     /locus_tag="PW113_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2525805.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="PW113_001489"
     CDS             11186..12328
                     /locus_tag="PW113_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2525806.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="PW113_001490"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="PW113_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2525807.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="PW113_001491"
     CDS             14128..14775
                     /locus_tag="PW113_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2525808.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="PW113_001492"
     CDS             15168..17813
                     /locus_tag="PW113_001492"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2525809.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="PW113_001493"
     CDS             18123..19436
                     /locus_tag="PW113_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2525810.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="PW113_001494"
     CDS             19426..20079
                     /locus_tag="PW113_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2525811.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="PW113_001495"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="PW113_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2525812.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="PW113_001496"
     CDS             20976..21176
                     /locus_tag="PW113_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2525813.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="PW113_001497"
     CDS             complement(21430..23109)
                     /locus_tag="PW113_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2525814.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="PW113_001498"
     CDS             complement(23111..23323)
                     /locus_tag="PW113_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2525815.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="PW113_001499"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="PW113_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2525816.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="PW113_001500"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="PW113_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2525817.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="PW113_001501"
     CDS             27298..27495
                     /locus_tag="PW113_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525818.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         9080 a         4672 c         5763 g         8046 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaagt tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg t
//
LOCUS       DANKHJ010000032        27216 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000032 DANKHJ010000000
VERSION     DANKHJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..232
                     /locus_tag="PW113_001502"
     CDS             <1..232
                     /locus_tag="PW113_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDL2525819.1"
                     /translation="FLFKMDSKWDKYIYRSKEVFLTGSYNETLQESLYDTGAIFNNEP
                     MVLKDFFGDYELVWNPKQLSEQGAENEFAEVH"
     gene            730..984
                     /locus_tag="PW113_001503"
     CDS             730..984
                     /locus_tag="PW113_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525820.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            1381..2982
                     /locus_tag="PW113_001504"
     CDS             1381..2982
                     /locus_tag="PW113_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2525821.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            3216..3515
                     /locus_tag="PW113_001505"
     CDS             3216..3515
                     /locus_tag="PW113_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2525822.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            3650..4027
                     /locus_tag="PW113_001506"
     CDS             3650..4027
                     /locus_tag="PW113_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HDL2525823.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLIC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            4076..6439
                     /locus_tag="PW113_001507"
     CDS             4076..6439
                     /locus_tag="PW113_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HDL2525824.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLEKIA
                     QGNYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEETVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            6757..7356
                     /locus_tag="PW113_001508"
     CDS             6757..7356
                     /locus_tag="PW113_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HDL2525825.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            7468..7692
                     /locus_tag="PW113_001509"
     CDS             7468..7692
                     /locus_tag="PW113_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525826.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            8075..8281
                     /locus_tag="PW113_001510"
     CDS             8075..8281
                     /locus_tag="PW113_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525827.1"
                     /translation="MIENRVLGFVECKDNLDDTLEVLESIIFNTKVLDLKAINGDMTT
                     HIILDEISAKEIGETLIAWANKEL"
     gene            8361..9215
                     /locus_tag="PW113_001511"
     CDS             8361..9215
                     /locus_tag="PW113_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2525828.1"
                     /translation="MMKSKFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            9228..9665
                     /locus_tag="PW113_001512"
     CDS             9228..9665
                     /locus_tag="PW113_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525829.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            9985..10428
                     /locus_tag="PW113_001513"
     CDS             9985..10428
                     /locus_tag="PW113_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2525830.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLFHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSIVLYT"
     gene            10574..10897
                     /locus_tag="PW113_001514"
     CDS             10574..10897
                     /locus_tag="PW113_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525831.1"
                     /translation="MARSRIAKIEQTKKDQKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLVYCKDKG
                     IMIPA"
     gene            11315..14944
                     /locus_tag="PW113_001515"
     CDS             11315..14944
                     /locus_tag="PW113_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525832.1"
                     /translation="MKKLKLISVALFSLITLSTARQSVVSAEGTSTKAVVEESGDKKV
                     AIDEKVKNEAEKATVVQGADGLMYVVVDENNPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFETE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRRALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVIFTIYSSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEITQDTPSTKPAEVKVT
                     NIYEVSVSEKVSDMNESQVLKNVWSADPSDYLQYDITTGNLKKSGERNVQSFTIEGFY
                     DNKNVDVSKSEVYVGSTNVTSSFDISNDTGNGKVVARAKATVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKTLTIVKNSKVTVGSKISKSANSNDVETVLYSRKITINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGEDIVLTTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTELVLSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSIDLPTEYLTKNKKINYSIDILLVENPDKNKFETETKDLRTVGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERANNNPVKEFNENINYKFTGNTKTKTGYGVSVD
                     LEPNYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     NTQNLSFQFPHMNIERKTGNIYADSQKNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKEDELLLEPVYPESTLPDGWNKNEI
                     DWLNK"
     gene            15004..15366
                     /locus_tag="PW113_001516"
     CDS             15004..15366
                     /locus_tag="PW113_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525833.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSMVFTDMVHKMLNGEPINFNFDYMLEKAQSNQVVAFLVSTYYFIEKSIFSLKDF
                     FLGIFTKLTTFYNKILKN"
     gene            15380..15532
                     /locus_tag="PW113_001517"
     CDS             15380..15532
                     /locus_tag="PW113_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525834.1"
                     /translation="MLDQNTDRSWWMIGAVIVGAILIGTAKVAFPEVFSTVITFFKNM
                     IPTKLG"
     gene            15600..16022
                     /locus_tag="PW113_001518"
     CDS             15600..16022
                     /locus_tag="PW113_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525835.1"
                     /translation="MIYVALFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETTD
                     YTFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            16034..17449
                     /gene="tadA"
                     /locus_tag="PW113_001519"
     CDS             16034..17449
                     /gene="tadA"
                     /locus_tag="PW113_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2525836.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSANEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            17466..18281
                     /locus_tag="PW113_001520"
     CDS             17466..18281
                     /locus_tag="PW113_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525837.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYFNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            18281..19078
                     /locus_tag="PW113_001521"
     CDS             18281..19078
                     /locus_tag="PW113_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525838.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKTDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIKPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            19096..19458
                     /locus_tag="PW113_001522"
     CDS             19096..19458
                     /locus_tag="PW113_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525839.1"
                     /translation="MQEQSVMEIIKWFIGLIMIMTMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDGRFTIESDQLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVALSFNGNSVSQIR"
     gene            19472..19618
                     /locus_tag="PW113_001523"
     CDS             19472..19618
                     /locus_tag="PW113_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525840.1"
                     /translation="MQENNVSRIIYLIGGLIILSLAIAGLVEHWPEIKLLIRNFYDNK
                     MIKM"
     gene            19630..19851
                     /locus_tag="PW113_001524"
     CDS             19630..19851
                     /locus_tag="PW113_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525841.1"
                     /translation="MEAIQENRKDTQETIEKNKKTRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            19870..20145
                     /locus_tag="PW113_001525"
     CDS             19870..20145
                     /locus_tag="PW113_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HDL2525842.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            20254..22080
                     /locus_tag="PW113_001526"
     CDS             20254..22080
                     /locus_tag="PW113_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525843.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSWGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELAVSPEEPEEVEEIETVSENEEEVEEIFDFEDEEEVENVQKGDLS
                     TSSQNELVAVNDSQVPPYMPSQTPNYSPQYQAPNVLQPVRKHETVQLVTLNEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDVAWKKPYDLDVKKDKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            22093..22809
                     /locus_tag="PW113_001527"
     CDS             22093..22809
                     /locus_tag="PW113_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525844.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     YYENINAQISCPLDRNGKPTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     KKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEVKSVDRRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            22822..25680
                     /locus_tag="PW113_001528"
     CDS             22822..25680
                     /locus_tag="PW113_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2525845.1"
                     /translation="MKLVQNNEVKERKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKITKLKNAMTKLAKAI"
     gene            25731..>27216
                     /locus_tag="PW113_001529"
     CDS             25731..>27216
                     /locus_tag="PW113_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525846.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDSGEGED
                     RKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERA"
BASE COUNT        10280 a         3390 c         5228 g         8318 t
ORIGIN      
        1 gtttttattt aaaatggata gtaagtggga taaatatatc tatagaagta aagaagtatt
       61 tttaactggt agttataacg aaactcttca ggagagcctg tacgacacag gagcaatatt
      121 taataatgaa cctatggttt tgaaagattt ctttggagat tatgaactag tttggaatcc
      181 aaaacagctt agtgaacagg gggcagaaaa tgaatttgct gaagttcact aacatagtgt
      241 acagaaaaac attatctgaa agaagaacgc cttcaaaaga aagtataaga acgattgggc
      301 cgtacagaag actaacgaaa gatcgtaaaa aaaaggaaag acaatgaaaa tgtctttcct
      361 tttttttgac tacaagtagc ctgaaaatga aatttcaaaa gactacaagt agtcttttgt
      421 agcaaaaata aagagccaac gaaagctacc aacaatcgtt gactcgtaag actgttcgta
      481 gtaagaaaaa tctttggcaa tccacgaagt ctttatttca taatttatat aaatgattat
      541 acagtcattt tcctgaattt gcaagtaaaa aagctaagaa atattgttta agcgtttcct
      601 taaaagaatc tttaaataaa tttttttcca attgaaaaca tgactacaag tagactattt
      661 cttttattcc aagttgacta caagtagtca aggtgatata atatatatat caagaaagga
      721 gaaacaaaaa tgtataacgt aggagatcaa atagatgaga tcgtaaaaat aggtaatgtt
      781 aaacttgtta aaagtaagta ctatatttca gaaactgaaa taatttttaa tactgaatca
      841 ggatgtattc taatttggaa actgcctttt cccaaagacg aaacaacact gaaaaaaata
      901 aatactttaa aaatcagata ttgcattcat gaaattattg ataaaaatac ttatgttata
      961 aatcaagtaa aagtgatggg gtaaaaatct ttaaaaaaaa ctcttgttaa aagtaattct
     1021 attagcttat aatgttggta ttagttctga agccaaagga acactaaaaa attctagtag
     1081 aaaagttagt tacagactct ctactatgta tgaatgtaac gaaaaaaata gcgaaaaaga
     1141 taattaaata agactaccaa tcttatttaa tggaacttct gtagtaagaa aagcgatttg
     1201 gcaatccacg aagtccacca agatgagcta ccaactcatt ttaatttttg cacgtttaaa
     1261 gtttagcatt taagtgctta ctctaaagac atagtgtaat tttaggactc cttattacgg
     1321 aatctaaagt taccctatgt cttttttgct attcaaaatt gatagcaagg agaaagtgca
     1381 atgttgaata gttgcgagta tgcggacgtt ttagctaaaa ttcttaaaaa tggaattaaa
     1441 cagtttaata gcaaaaatct agttgctttt aaaaagaata gcaataagat tggtgctatt
     1501 gccggattta cttctaaaac aaacatgata aagactaaag gtcttatata tcgttctatt
     1561 gatcgtgtct tggaagattc agatgtatta acacactgga ctcctaacgt gtattcatgg
     1621 cttggtggcg gtgaaaacaa aacaattttt ggtcacaaag aagctaactt aattcagatt
     1681 aatacttttg tagcagatat tgactatcca gtaggagaaa agaaaacgga tttgaattta
     1741 ctagttcttt atctattgga agaagggtta ttgcctacac tgattgtaga tacgccaaaa
     1801 ggttatcatt gctattttct gattcaaaac tataaccaag aaactgattc atttgataag
     1861 gcaagctata tttctaattc aaatgatttt aaatctttga gagttgctaa aagaatatca
     1921 gaaaatatca gaaaatctat ccaaaatcgt ttaccacaag tcgatatggg ctgtaatcat
     1981 tttggtattt ttagatttcc aactaaaaaa aatattgtac attatgaacc taatttcgta
     2041 gatacgtttg aagggtattt gaaatggtca aaagactttg aagaaaaaga atatgcttta
     2101 aagaggtcta acttgtcatt attagctcct aattctactg gcaagaaagg gtctaaacga
     2161 caaattgata ctaaatggta cgacttttta attcatacgg atattgctga aggagataga
     2221 aataaaacta tttttacctt agccctggca tgtaaatcaa gtggcttatc tattggcgag
     2281 tgtatggatc aattggatca acttcaattc aataatgatt tgtcctattc tgaagttgag
     2341 agaacaataa gaagtgctta tcgtggcgat tacaaaggtg ctaaaagttc atatgtcaat
     2401 gaaattatcg aaaattatgc tacacctacc gagtttgaaa aatactcaat aaatgtaaat
     2461 agacaagctg ttaaacgtaa agggaatcgt gtcgatccgt ctaattgggt gaaatttgct
     2521 aaacctagag aagaacgaaa atatagtcat tttgaagaaa gtaaagctga tttattagct
     2581 tacttagaaa gaaaacaaag atcattgaaa aaaaatgagt tgtttttaag tgtctctatg
     2641 ggaactatat ctgaagaaac acaaattcct gtatctacgt taaaggtgat tttaaaagag
     2701 cttagaacgg cgaatacagt gatattacga acgtctcgtg gtcggtatgg atcgactaaa
     2761 atagcaacaa gcaaatttgt ggaagctaaa atattaaata cagttattca gaataaaaaa
     2821 gataaacaag tatttgtctt agaattatta ggaacagcaa atagccacgt agaaacgatt
     2881 ttaagacgtt atgaagacaa aacagtaccg ttccatgcga acatactgaa agaaagagaa
     2941 aagagcaaac atgcgccaat tagaggagct aatacaggtt agagttctat ttattttttt
     3001 gatttttaaa tagctaaatt gccaattaac atatctagtg tttctgccca ctcctattcc
     3061 tgttttagcg tatactaatg attagtagtt agtactctct tttatgtatg gagtgtatag
     3121 aactattttt acttgtgtct tttcccttaa tccacgtgta cggaatacag cttataactg
     3181 cgtatgcagt tatgtttgaa ggaggaagat tttctgtggt aattaagaaa tatgtttact
     3241 cacacccgat caatgtattt attatcggta agctaggttt atctgttgag gtgttttgtg
     3301 agttatatgg atttaaacaa ggaactctat cgtcttggat tactagagag cgtgaggtag
     3361 ctgatttgcc tatttctttt ttgtatgctt tgtctctagc ttcaggaggg aatatgaatg
     3421 aggtttatga ttgcttgtgt gagtatgaaa aagagtataa agagtatttg aaaatttcag
     3481 ccccaaaacg gaataaaaaa tatattgaag attagagaaa gaagaaaaga ataatttgtt
     3541 ttttgactac aagtagactg atagctactc tgttttttga tagtttgata ttggctgttt
     3601 ttttgttaga attaatatat atcaggtaaa taaaaaaagg gggctattaa tgaatattga
     3661 tgggatttat aatgaatcag attttaaaaa aggcatagta gatattgata gaaaagagta
     3721 tttatttcct gatgaaatag gtttatttaa aattgtaggt gaaactgtct gtaggtattg
     3781 ggggaagaaa ggaaatataa ttttacttgt tgatacgatt gagggaagaa agttaaaatt
     3841 ttcgttttgg agtaatacaa aatattgttg tgaacctgta cctaaaaggg aagtagatgt
     3901 ggctaaagaa agtgtacgat ttttacctga tcgatcaaaa ataactttaa tttgtgaacg
     3961 aacaagtaat gctaaattta caagaattag agatatatta ctttcgacta caagtagtca
     4021 gtcatagagt aagtactttt ggttaaaaat caattgaaga aagtggagga agtatatggc
     4081 agtagttata ttagcagaaa aaccagatca aggagcaaaa tttgcttcag cattggccgg
     4141 aaaaaaatta tcgaaaaaga gtgggaagta tgaatttaaa agtgaaatat ttggagatac
     4201 tattgttact tggggaatag gtcatttggt aggattatca ttacctgaaa agtatgagtg
     4261 gctaccgaat aaagataaat ggcatttaga taatttgcct tttctaccta aattcaatga
     4321 attaaagtat gaagtctcta aaggaaaaaa ccaacaattt tcaatagtga agtcatgttt
     4381 agaaaatgca gatgagataa taattgctac tgatccggat agagaaggag aaaatattgc
     4441 ttataatatt ttcaaattat gcaagcagtc gatttttaat aaaccgataa aaagattatg
     4501 gattaattct ttggttcaaa aagaaattat caaaggattt aaaaatctgc gaaattctga
     4561 agaaacgatc tcttacttca aagaagctaa tgctagacaa attgctgact atctagtagg
     4621 gatgaattat acagagttgt attcattaaa gttgcaagaa aaaggtatat cgggtgtggt
     4681 atcactcggt cgagttcaga caccggtaaa cacacttgtg gttgaaaacg atttggcaat
     4741 tagaaatttt agaccggaaa aatataaaac gattgaatgt catacaaccg ataagcaacc
     4801 aaaagcaata tttaagaata gtcttgaata ctttaaatta gaaaaatttg aagaagacac
     4861 taaaaaattc ggccttgatt ccgccagtac tggaatcata aaaaaaattg aggttagtaa
     4921 aaagcaaaca gaaccacaaa aattgtttag cttgggtggt attcaagaat acgctaataa
     4981 aaaatggaaa tattctgcta aaaaaacaga taaaattatc caaagtctat atgatcgtgg
     5041 atatttatct tatccaagaa ccgatagtga attgataact acaaatgaat ttgagtattt
     5101 aaaagcgaat ttagacgtgt ataagagtgt tttagggatc agtattgata caccttgttt
     5161 agagcctaaa aagcggtttg tagataatga caaagtatta gagcattatg cactaatacc
     5221 aacaatggat attcctgata tagcaaatct gacattagat gaaaaaaata tttatgagat
     5281 cgttactagg cgtgcgtgtc taatgtttgc agaaccttac aagtacgaaa atacaaaagt
     5341 cacattagat gtaaacggct tagaatttac cacaagtggt aatgtaccta tatctttagg
     5401 ttggaagcaa gtagatacta atttagatga atctgatgat aaagaggaaa cattacctaa
     5461 atttcatgaa caagagacaa taaatataga tattagattt attgataaat taactaagga
     5521 acctaaaagg ttgactgaag gtaaattatt aggtaaaacc ggtattatgg cgaaattagg
     5581 gctaggaacg cccgctacac gttctaatat tgtagacact cttctaaata gggaatatat
     5641 taagatagaa aacacgaatg tttatcctac tccaaaggga tatttattat gggatttgac
     5701 aaagaataaa gacctattga ttggaaaacc agaaaataca gcgatatggg aagaatcact
     5761 tgagaaaatt gcgcaaggta actatacaca ggaagctttt ttaagaaata tctataacta
     5821 tatcaataag actgtttcag atttgaaaaa agtagaattt acttcggatt atttagaaga
     5881 gactgtaaat caaaataaat ttgatatagg tagttatcaa gtggaagaaa agaaaaaagt
     5941 ttattctatt ttaaatatta ctagtggtga acagttcgtg atatttaaaa atatatcagg
     6001 aaaagcaata aataaaaaag ttcttcaaga actattacta aataatcgta cagataaagt
     6061 cctttccggc tttaagtcaa aagaaggaaa agcttttaat gcaagattac ttttcgatcc
     6121 aaatgtgatg aaagtggtaa tggagtttga aaagaacagt ccaaaaggcg ataaaaagag
     6181 caaacacgat acagtaaatg gttatgaaat agttgaaaaa gcaaaagtat ttgaaataaa
     6241 agaattagca acaggtgaaa gttttatttt ctataaaaat aatagtggga aaactttaag
     6301 ttcaaaaaca gtcaaagaat tgttagaaaa tgggaaaact aaagaaaaag taacgggttt
     6361 tataagtaaa gacaataaaa aatatagtgc ttatcttgtc tttgataata aaaacaaagt
     6421 tataaagaaa gaattttaat tcttagtggc ggaaaacatt tgtttttccg cttattttta
     6481 ttcaaaagtg cccaaaatat aggtatactg aattaaggat aaatacaaaa ctagttagag
     6541 gacagttaaa aaaatacaaa caaattagat tgctattttg ttaaattgga agtagaataa
     6601 taccgttttg atatgtatag tgtgttttac caataaaatt acataaacgt gaatatattt
     6661 tcatgaatta aatgtaaaat gagagcaatt agttttaaaa aagattatag gctgttaatc
     6721 ttagcatata taatccattg gaaaggggga gatccaatga atctaaagaa tacaaactca
     6781 aacaaatcta cactatatgt aatttcagat gattatataa ataagctgaa aaaggtggac
     6841 tatcgtgtcc aaaacaacta tcgagaacaa cgattatatt ttaaatcaaa cttgaaaatt
     6901 tctaacaaaa atgaagaaat aaattattat gtaccattgg cttcagcaaa agaaaatcaa
     6961 cgttctttga ctaataaatc agtgtttaag atttatggtg atcgagaaga aaaggacttt
     7021 ttaggagtac ttcatataaa taatatgatt cctgtgccaa aagattgtgc aaaagtatgg
     7081 acacctaata gcccaaatgt tgatgagaag tataagatgt tagtaatcaa acaggcaaaa
     7141 tatttgaaat tacatgagtc tgaaataaat gaatcatgcg aattacttta taattctaag
     7201 ttaggtaaac ttgatcctag ttactttaag aaaaatcgta aagaagtaat tacttatcaa
     7261 aaaataatga cgaatgtaga atcattggaa gctgttagta tagcagaatc ccaaagaaat
     7321 attcatgtac aaactcaaag tatagatgta gagtaagata ggctaaacct atcttatttt
     7381 attattctat tggatattat gatacattga attttaatcg aatatgtatt taaatgttat
     7441 tatactgtag aagataaaac ttaaaggatg atttctatgg cgagaccgag aaaatacaat
     7501 acagaagaag aaagaaaaga agcaatgaaa aaggctagga aaaaatgggt tgaaaaaaat
     7561 caggaaacta ttgcctatat aagagccaaa tcatttgcta aaaaattcat tgaaatgtct
     7621 gaagatgaag atattgaatt gttggaaaaa tggttagaag atcggaaaaa taacggtcaa
     7681 aatgatgaat aataaccatt gactacttgt agtcaatggt gtagaatggt tttagggata
     7741 gaaatatccc ttttctaaaa gtctgatgac tacaagtagt caataggggg atcgggaata
     7801 aaaacagacg attatggcct aaagaaagga ggaatttgaa tattttgagg aacgatttat
     7861 taaatgagcg ttgataaatt tcactaaata ttttcttaat atctatgtat tgttcaagcg
     7921 tagaaaaaaa tattgaaatt attacaaaac cgctcataat ggaaaataac gaattatatt
     7981 aatgaatcgt tcactattga ccttttttac tagatttttt ttgtaggact tttagagaat
     8041 ttttttgtta tctgattata aggaggagct aaagttgata gagaatagag tactggggtt
     8101 cgtagagtgt aaagataatt tagacgatac tttagaagtc cttgaaagta ttatatttaa
     8161 caccaaagtt ctggatttga aagcaattaa tggtgatatg acaacacata tcatattaga
     8221 tgaaattagc gcaaaagaga ttggcgaaac tttaatagca tgggctaata aagagctgta
     8281 aaagataatt ttcaaataaa gtggccaatt gactacaagt agtctgtata gtataataaa
     8341 ttatcaaaag tgaggtatac atgatgaaat caaagttttt gggatatgta attgcaatca
     8401 acatgtttaa agggggagtt ggtaagtcaa ctcttactca gctaatggct gatattctat
     8461 cggctagagg ctttaaagtt ttagttattg atgttgatcc acaaggaaca gtaactaaaa
     8521 agtttcaaag acaatatgat ttaaaagttg ttgggaaaat gacattgttt gaagcagtaa
     8581 ttaatatgtc tttcttagac tgcatttctc ctgttaaaga aaacttagat attgtgcttg
     8641 gttcagaaca aatgtcaagg tttaccaaag tattaagaga tatgtggaga agtactggtt
     8701 ttactgatga aggtgaagaa gtcttttgga atttgtttaa ttatatgatc ggtaatgata
     8761 atttaagaga tcaatatgat tttattttgt ttgacactat tcctacagtt tcagaattta
     8821 ctgataacgt ttacgtggcg agtgattttt tagtaattcc cacgcaaact gagcaagatt
     8881 cgatagacaa cttgcacagt acggttacta attatcaaac tgctgtaaaa gaagtaaacg
     8941 gagatttaga aattttaggt attgttccat atctagtgga tactaaatta aaaacaggat
     9001 tagaactttt atcagaactt caagaagaat atggagactt agtttattct catattgttt
     9061 cagaacgagg cgtaattaaa cgttggggta gagaaggact aaataaatct atcccacatt
     9121 ctaaaactgc ttatagcatg tatgtagaca ttgtagaaga aacgttagta cgtttagaaa
     9181 aagctacagg agaaaatatt ttagaacgtg tttaggaaag gtgttgcatg atgtcgaaaa
     9241 ctaaaaaagt aactagtgcc ggcagtaatg gttcgggtaa tccgttcgga ataaaaagcg
     9301 gtagtggtgg atttaatata ggacaacaaa tgttaaaagg taaagctggc attaagtcta
     9361 ctggtgataa acggaaatct gttagggtac cagttgagtt atacgatgaa gttgttaaag
     9421 atttatcata cggtgctaaa tattcattaa tgacagaaat tttagcttta tttttaaaag
     9481 aatttgaaac acacggaaat agtatagtta ctgattttgg caagttgaaa acgagaccag
     9541 taaaaatttc tgaagaagcc tttaaagttt tgaaagttat aaaatttgaa acagatcaat
     9601 ctaatactaa tattattgct tcagctttga catggtatct tgataaaaga aaaggaaaag
     9661 cataatgaaa tgaggtaaga ctatggatca taaatcaaat tattcataca aagaaaatta
     9721 tatttggaag tatgtagtag ccgtttttct actggtaggt ggaattattg cattggttaa
     9781 atgggctata attcctaatg ctttagtatt tatcaattgg tttgtcccta atttttggag
     9841 tttttttggc tatatattga taatctggtt tatgtttaaa gtactctctt ttttctggtc
     9901 gcctgtatat tctttgcttg gaaagtggtc gtacctaatt ttaatagcga cagctattta
     9961 tatcattatg cagattaact aattatgcag attttagaag atattaaaat ttattggaaa
    10021 aggataatag gctatacatt agttcttctt gtagtaaatt tattcctttc gatgatagcg
    10081 ttggtctttg ttaatcaaag aaaagacaat gtaactgaat tagtaacgtt tatttcgttt
    10141 ttcataagtg ggatttttgc tcctttcttt gaagaaacct taatgagagg gattatccaa
    10201 aaccaattgg aattaaaaac aaatttatct acaagaagca tatatataat cgttgcaatt
    10261 ttgtttagtc tattccactt aaatttatat tttattcctt attttatgac gagtttaatt
    10321 ttgtcttttg tctatgataa ttcagataaa aggctgattg ttccggttac tattcattgt
    10381 ttttacaatt taattgtaat actgttaagt atagttttgt atacttgaaa aaaatagacg
    10441 aaaacctaaa aatacaaggt ttttatctat tttttttgtt ttgtttttat ctatcaggtt
    10501 gatagaaatt gtcgtaaatg atataattaa gtatgaactg atttagtaaa aaaattaaag
    10561 gagtgaatag ctgttggcaa gatcaagaat tgctaaaatt gaacagacta aaaaggatca
    10621 aaaaatttct aaaaataaaa caaaattctt taaagctgaa gatgttaaga ggatagcatg
    10681 gtctgtgaaa acatttgatg aagatcgtta tgttaaagtt tcaatgaatg gtctgtttgt
    10741 acagcttggt aacttcagta attttaaaac gattgacgaa ctttatccgg aaattagaaa
    10801 tattgtggat agtaaaggtt taatcgtggc taaacggaaa tcaagaaact ctggtcttgt
    10861 ttactgtaaa gataagggaa tcatgattcc agcctaaatc agtgaatttg ttccttgaaa
    10921 acttaatact actcgtactg tagcaatgtt gctatgttac agataaaagc gtagggctaa
    10981 caagcaaaat agcaaccaag catactgtta tttcctttgc caagaggtat gtaaatacgt
    11041 tagcgataga aatgataagg ttagtatttc acgccaaaaa atacaaaagt cgatccattg
    11101 aaactactaa ttatgaggtg gcgtaaatgg taattattca aagtttctat ctaggaagaa
    11161 cctatgaaaa tcgtcctaga gatcagaata ttgacactaa aaaattcagc acagtaataa
    11221 atgtgctaga cacagggtaa ctctttacta caagaaatgt agtaaagagt tgttatgatt
    11281 ctttattaca tttctttcta aaggaagtgt gataatgaaa aaactgaaac tgattagcgt
    11341 tgctctattc agcctaatca ctttatcaac ggctcgccaa tcagttgttt cggctgaagg
    11401 aacgtctacg aaagcagtag tagaagagtc tggtgataaa aaagttgcta tagacgaaaa
    11461 agtgaaaaac gaagcggaga aagcaactgt tgttcaaggt gcagacggat tgatgtatgt
    11521 ggtcgttgat gaaaataatc ctgaaaaatt gctagaagct gaatctcctt tgaaaaatca
    11581 acaagaaaat accggcgctg ttgaagtcgg tgttgatgaa aagagacaag aaaaggctga
    11641 taaagctaac gaaaaagtga aaaactttga cgatattgat tacaacgata ttagttcgtt
    11701 gcagtcagcg ttgaatgatc cgaaattccc ttttttatcg gaagatgaat taaatgaaaa
    11761 attgcaaaag acaaagaata catttgagac tgaagcgtta atgaaaaaga caagggggta
    11821 tttcagtcaa aggattttag agttaagcaa taatttaaat gacgtaccga aaagagcgaa
    11881 agtgagtcgg cgtgctttag ttagtaaccg tgcttcacaa gtaagtgaaa cacgaataaa
    11941 ccctccaaat ggttatccgt gggtgattta tttaattagg agcaatggtt atagttatac
    12001 agaaaattat gctattagaa gaactattga tgggaaagaa gctttttgtt tacaggccgg
    12061 tgtacaagcg acgaatacat acactggttc aacaagtggt tatctttcta attcgcaaag
    12121 agaaatgatt agttcgattt tatcagcata taaagaatta ggcggttggg ctaataatga
    12181 tgccggaaaa caaagatgga tagcaactca aatgcttgtt tgggaaaaca gtaatgaaaa
    12241 gcctaaaagt ataactgtta cgccaaattg gaaaaacgga gctactaagt ggaaaaatga
    12301 tttagtggct cgtgcaaatg ctattagagt aagaacaaaa tgggattcta cgtctaagac
    12361 ggttgatgag ggagacaaag caacctttaa agtaactaat acttatggag atcataattt
    12421 gcatgtgaaa tctgtatctg gtggcggcaa agctaccata agcaatggta ctttaactgt
    12481 tgatactaca ggagccactg gaaacaaaat cactgtagat atgattaaag gttctcaccc
    12541 ttcagacgcg caccctacaa cggtatggag taatggttct tatcaaaagc ttgttaccgg
    12601 ttctttttac attagtactt ctgttacggt aaatgttaag agaaaaggaa ctgttgaagt
    12661 tttaaaagtt gattcagaaa caaaaaaacc attatcagga gcaacattta gatttagcta
    12721 ctcaggtaaa acaaaagatg ttactactga ttcgaatggt aaagcaaaat taactgaaag
    12781 gttgaaagct ggaactgtag taaaagttaa ggagataaaa gctcctaatg gctaccaact
    12841 agattcaagc gaattttcaa ttacagttaa agaaaatcaa aatgttacta ccacaagaac
    12901 caataagaaa tccacaggtt ctgtagaaat tgagaaaata ggcgatttag atggattagg
    12961 attgccgaat gtaatattca cgatttatag ttccgataat aaagttgtaa aagacaactt
    13021 aaaaacagat agtaatggga aaataaaagt tgatgaacta caatttggta aatattatgc
    13081 agtagaaaag caaggtgtta ctggttatga tcccgatgga aaaaaataca attttgaaat
    13141 aacccaagat acaccttcca caaaaccggc tgaagtgaaa gtaaccaata tttatgaagt
    13201 atctgtaagc gaaaaagtat cggatatgaa tgaaagtcag gtattaaaaa atgtttggtc
    13261 tgctgatcca agcgattatc ttcagtatga cattacaacg ggaaatctaa aaaaatccgg
    13321 agaaagaaac gtccaaagct ttactatcga aggtttttac gataataaaa atgtagatgt
    13381 ttcaaaaagt gaagtatacg ttggatcaac aaatgtaaca agctcttttg atattagtaa
    13441 cgatactggc aacggaaaag tagtcgctag ggcgaaggca accgtgttaa cgaataatga
    13501 tttttataat aagtcctata atttacggat cactatgaaa ataaaaaaat ctacgcttga
    13561 aaacactgaa aagcaaaaaa ccttaacgat tgttaaaaat agtaaagtta ctgtcggttc
    13621 aaaaatatct aaatccgcta actctaatga tgtggagaca gttttatatt ccagaaaaat
    13681 tactatcaat catatcgatg aaaaagataa acaccttttg aaacaagata ttgattataa
    13741 atacgatggt gaaacatatg aatataaacc tagaacagat ttgtttgata aagatggaaa
    13801 taattataaa tctgatgtaa ttcataaagg gaaaattgat ggagaagata tagttttaac
    13861 cactccttat catattccgg tgctttctgt gaatgtggat cggattcaaa ttgatacggc
    13921 tagagctgtt aaaaatggct atttacctac agaattagtg ctaagcaaaa aagaagaata
    13981 tgaaaaagag ttagaaaaaa ttgtatttaa agtgaaaatt acagatatag ataacaataa
    14041 agttgtttat gatgaaaact tcaaatacaa agaatttaat agtcatatgt cgattgattt
    14101 gcctacagag tatttaacaa agaataaaaa aattaattat agtattgata ttcttttagt
    14161 ggaaaatcca gataaaaata agtttgaaac tgaaaccaaa gatttaagaa cggtcggtta
    14221 tacttcttct gaaaaagttt taacgaacaa agatatgaca aataaccaag tcaactattc
    14281 agcagtagtt agaacagtga aagaaagagc gaataataat ccggttaaag agttcaatga
    14341 aaatatcaat tataaattta ctggtaatac taaaacaaaa acaggatatg gtgtatcagt
    14401 tgatttagaa ccaaactaca gaaatgaaat tgggaagcaa atagatatga aagtctatag
    14461 ttatccgatg aatgaattga ttgacagcaa aattgacgaa atctatcaaa gtgggggaaa
    14521 agaaacagag gttaagatgg atcgcttaaa gaatgatggg aaagttgagt cgggtattaa
    14581 cactcaaaat ctttcattcc aatttccaca tatgaatatc gaaagaaaaa ctggaaatat
    14641 atatgctgac agtcaaaaaa atgatgaatc tattaagtat gaattgatcg atggcggtag
    14701 aaaattatat gtgcctattt ggtgcgattt ggggaattat tctattgcta tcaaaagtag
    14761 taactttggt ggtaactata tctcgttgga tatgaaccag aacatagatg tttatgctta
    14821 tatgtatgca acgatagata gtgaaactaa aaaagaagat gaattattgc ttgaacctgt
    14881 ttatccagaa tccacattgc cagatggttg gaacaaaaat gagattgatt ggttgaataa
    14941 ataagtttga aaagttttta tctatctgat agaattaaac aatgatatag ggatggtgat
    15001 agaatgaggg ttcaaagcta tggtaacagt gtttttgatg ttaaaagaga atttcaagat
    15061 tcgagaagaa aaaccaaaaa gctgatagct tacttcattc tatcagctat cgttttctat
    15121 attgtcaagt ttgttttgga attaaactca atggtattca cggatatggt tcacaagatg
    15181 ttaaatggag aacctattaa ttttaacttt gattatatgc ttgaaaaagc gcaaagtaat
    15241 caagttgtgg cctttcttgt gagtacttat tattttattg aaaaatctat tttttcttta
    15301 aaagactttt tcttggggat atttaccaaa ctaacaacgt tttacaataa aattttaaaa
    15361 aattaaggag gaagtttgaa tgttagatca gaatacagat agaagttggt ggatgattgg
    15421 tgccgtaatc gtaggtgcca ttttgattgg aacagctaaa gtagcttttc ctgaagtatt
    15481 tagtacagta attacatttt tcaaaaacat gatccctaca aaattaggtt aattccacta
    15541 aaaaattcac gctgttaaat gcgtagaaaa ataataaaga agaaaaaggg ctacataaag
    15601 tgatttatgt agcccttttt ttggatggaa gtgatgtaat gctaagagat atgcccaaaa
    15661 tgattcttct gatgtttgtt gggttctttt taattttagt gattgcgtac tattcattaa
    15721 atggctatac acaagattct tcagtgaaag gtatgcaaga aacattaaga actacagcaa
    15781 tagcaaacag ggatgatagt attcgtgtag aaaaagggcg ttttgcttta attaaatctg
    15841 attttgaaaa agattttaaa gaaaaatttg ggaaaagtaa aaatctaaat aatgaaacca
    15901 ctgattatac atttgattat cttacagatg gaaaggatgg tattaaagca attaaagtga
    15961 aggttgaaag tggatcaaaa gaatatcaag caacttgtat tttaaatgtt gcaaatgatt
    16021 agaggtgtga aatttgcgtg ttgtagagga aaagaaaatt tctccaaaaa tcgaggaaac
    16081 tcctaaaagt aaaattaata tcgcaagtat tattaaaaaa aatggaatta aagaaaactc
    16141 tgaagcgata gaaggaaatc ttgatttttt aactaagctt ttagaagaag atagtggaaa
    16201 ttatgaacca ccaattgaag atgcaaaatt gaatatgatt cgtgatgatt taacaagaaa
    16261 ctattcccaa gaagttttac aagctttaac tgatgatgaa attaggcgaa aacttgtaca
    16321 agttgttttg aaagaacatg gtactttctt taaaaaagat aaagaggttg cagaatatgt
    16381 agtacgtgaa tgtattggaa cagggattat tgagcgaatt atgaccaacg atgaaattac
    16441 agatgttggt tggaatggct ctcaacttag cattgaaact aactctaaca aatggttgtt
    16501 tgacggtgat aaattaaata tcacgcaaga gtatattgaa cgtgttattt ctaaatatgc
    16561 tgttgtaaat gatcgggctt ttaatagtgg taatcctatc atggatggaa tgtttaaaaa
    16621 tgtccgtata tcagctacac acatagataa ttcaccagat ggctctacgt tttctatgcg
    16681 tgtggcaagg cctagattag cattgaataa agaaaacttt gaagattttg ctcctgatta
    16741 tattttggag ctatttgaga aaatgatgaa aacaaaggca aatatctgta tatcagggga
    16801 gacaggtaca ggtaaaacgg aacttcaaaa attactgcta agttttattg ataatcatga
    16861 tcgtatgatt atgatagagg acgttcaaga aactcatgcc aaagaattgt ttcctgataa
    16921 agatattttc tcttggatca ctactcccaa aaaagcaatt tcagatttag taaaagcttc
    16981 tcttagaaat aatccacgat ggatcattgt ttcagaaaca cgtggttctg aagcttatga
    17041 aatgcttcaa gcggttttgt caggtcacta tgttgtaacg acacttcatg cggttaatgc
    17101 acgtgcgata cctagacgtt ttgtaaatat gtgtgcaagt ggttttaaaa taaacgaaaa
    17161 tgcggttgag gaagacataa atcgttattt cgattttggt gttcatatca aaaaaacagt
    17221 ggttgatggt aaagtgatcc gttatttgaa tgaaattgtt gaattttctg ctaatgaacc
    17281 gactacagtt tttaagcaaa aatttatcaa tggaaagttt gtcattgaaa atggtagtct
    17341 ttccgaagat tttaaagaaa aaatggctga aaaatttatt acgtttgaat ttcctgaaga
    17401 tcaaactaat acagaagaat cacttcctga agaagaagag gtgatttaag ttgaaagtac
    17461 aggctatgac aaagcgtaaa ggtctcatgg atgtttcgga actaaacgcc tatcaagtag
    17521 cgtatggtaa accgttagat tatagagatt attttaacta tgttggatta ccttgtttgt
    17581 ttatgggtgg cttttctttt gctttatgtt actactggtg gattgctttg atttgtggag
    17641 taattggagg cgtatatggc tttaaagtta taatgcctaa aagtattaaa cgctcttatg
    17701 aattaaagag cttacaggaa agaaataaat ttattaacaa catgactcaa attttgaccg
    17761 atgatagtaa aacagttact agagcgttat ctatagcaaa acagcgaaca aaaggtgaat
    17821 taagagaaga tattcaaatc ttagaatcac gtttacaagg tgctgataaa tttcagattg
    17881 ctgaagcttt tcgtgaaatc aacaataaat attatagtga tgttgttttt acacaatact
    17941 ttgagcaact ggaaacagct atttacgaag gtagaaataa ttcagatact atgaagcaaa
    18001 taaaaagtta tcacaatgat gtgaaacgta aaacagaagg ttttatgaaa atcaaagatg
    18061 gctatcttca agatatgaag caaatgatgt ttattgtagc tgtttttatt ggagctataa
    18121 cttttgcttt tgggttttct actttctatg aaggctttgc tcgatcacct atcggatggg
    18181 tatttgggtc tatttacttt ttgattatgg ttagcttcat gaagtcattt ttcaaacatt
    18241 attttgatga tgaaataatg agtttggggg taagtaatta atgaaagtta aggctttgaa
    18301 aaagaaaaga aaatcaagta ttagcccctt gaaaattacg aaaacagaac ataaaagctt
    18361 ttggattaaa ttgctaggtg atgtttatta tcaaaaatta cgtgaaatga acaattccaa
    18421 tattgcgatt attaagtggc aaaaacaacg gttaaaaaag tcattgattt ttatcctggt
    18481 cggattctta atgtatctat ttttagggaa agaatggtat tacttagccg gaggaattgc
    18541 tacttcagta ggtttttatg tttggcaagc aaaatcaatc aatggtatct ataatcaatt
    18601 tcggtttgaa cgacacttgc aatttagtaa atttacacgt ctattgattc catatctgaa
    18661 acaaaaaaca gatggcggaa atctatacgg tgtttttgca aaaatcgtaa aaagactaga
    18721 ttatgatacc gataaaaagc tattaatgaa gcttatgcaa gaaatgacag atcagcctaa
    18781 cgatattaaa ccttttacgg aatatgcaga acagacttcc ggaacagata tgtccgtatt
    18841 atttatgtct acagtttatg atattcgaca aggatcggca gatttagatg tgattgaaga
    18901 gttggatcga ttagcttctg aagaattgat ggccggaatc gattcaatca ttgactataa
    18961 gtcgaggcgt tttgtcttct ttcctacaaa aattgcaatg acttcattta ttttagttgt
    19021 cggttttgca gtaggcgttc ttttcatgaa cctaaaagat attaactttg ggcaataaag
    19081 gaaaggagaa agtcaatgca agaacaatcc gttatggaaa tcattaaatg gtttataggt
    19141 ttaattatga ttatgacaat ggtttctata ggactctttt gtgttcaatt acaagatatt
    19201 aatactttta agcaacaagt aaattaccaa attgaacgaa aaggaggtct gactacagaa
    19261 gctgttgaag caatcgacaa gtattcggct actaattatg atgggcgttt tacaattgaa
    19321 agcgatcagt taaatcagaa ggtttcttat ggagaagaaa ttgattatac gatcaaagca
    19381 actattccaa ttcaaatttt accgcttcct gatgtagctt tatcgtttaa cggaaatagt
    19441 gtttctcaaa taagatgaga ggaaggataa aatgcaagag aataatgttt ccagaataat
    19501 ttatttgatt ggcggattga taattttatc cttagctatt gccggtcttg tagagcattg
    19561 gccagaaata aagttattaa taagaaactt ttatgacaat aaaatgatta aaatgtagga
    19621 ggtatagatt tggaagcaat tcaagaaaat cgtaaggata ctcaagaaac aattgaaaag
    19681 aacaaaaaaa caagaaaaaa gaaagtagat aaatttacgg aaatgaccga taaaatcatt
    19741 gaaaagaaag gacttaaacc taaagacttt gaccgacaag caatcaaaga agcacgacaa
    19801 aaactgtatg atgattttgt taaagaaaac atatcacagc ttttagacta acatttaggg
    19861 ggaaataaaa tgaagaattt agttatcagc aagtttgcaa tgttaccaag aatggatttt
    19921 ttcgataatg taagtaagtt tatgtcaaat atgacttcaa aactacaagg aatttcgcta
    19981 tctgtaatta tcttttgtat tgtggttact ggtttgatgt ttcttttcgg agaagggcca
    20041 agtcgtactg ctaaaaaatg gttaacgtat atcgtgattg gggcaataat tatctttgga
    20101 gcttcaacta tcggacaaac gatcaagtcc gttggtggtt tctaagggga agcttcttag
    20161 aactaaaaaa ttcgcaagta ttgcatgaaa gagcattgag tgattaatgc tcttttttat
    20221 tttgaaaaac tataaactaa ggagatttaa actatgaacc aacgattcga atataatttt
    20281 gaagatgtag aacctaagtt gttgaaaaaa ggaaaagggt taaaaaagaa aaataaatat
    20341 actctcaaac aagcagaagc actagtagga tggattcaac acaattggtc agacaagaca
    20401 acaattacct tgatctgttc agatacagaa gaaggaacaa agtatgcttt tgatggaatt
    20461 gaaattactc cattttcgtg gggcggacaa gaaaaactat tgtttcctga tattgaaggt
    20521 caaattttac aagcgcctga cagcatgatt tccgatccta ataaactgat tttggatatt
    20581 aacgatcaat acaaacacga accagacgta gcagaaccaa caaaagaatc aaagtcaaaa
    20641 ttaaatctga agctcccttc agtagtatta ccatttgcta agaagaaaaa taaacttatg
    20701 aatgaattga ttgaaccgga agaaacggat atttatgaag aagaattaga agaacaagaa
    20761 gagattgaag aattagctgt ttctcctgaa gaaccagaag aagtagaaga aattgaaact
    20821 gtttctgaaa atgaagaaga agtagaagaa atttttgact ttgaagatga agaagaagtg
    20881 gaaaatgtac agaaaggcga tttgtctaca agtagtcaaa atgaattagt agctgtaaat
    20941 gattcacaag ttccgccata tatgccttct caaactccta attactctcc acagtatcaa
    21001 gcgcctaatg tcttacaacc agtaagaaaa cacgaaacag tgcagttagt tacgcttaac
    21061 gagtactgtg atttgggaga agaaattgaa acttcaattt ctcaaatggt cgaaaagttg
    21121 aaaccagaat ctttattcaa attcataggg attccttcta caggtcttac aaatactcgt
    21181 attgatgaat atagaagtaa ccatgctatg ttgcgattag ctgaagtaaa atttgaaaat
    21241 ttaagagagc attattcacg acaaattatt tcttttaaag cagaagcact agatcgttta
    21301 agaactgcgg tagatgtcgc ttggaagaag ccttacgatt tagatgtgaa gaaagacaaa
    21361 gccgaagagt tatcaaaaat ggaagcagaa gcagaagcta aattaaatga gttcgtgaaa
    21421 cgtcaggaaa acaatgtaaa tgataaaatc aaaaagtttg agatcgaaca agaaatagat
    21481 ttaaaaaact ttattgcaag acaagaagca gacaaagcgg tattcattgc tacagagaaa
    21541 gatcggactc aaaatttaat tgattctaaa gcggaatcaa tcaatgcaga tttacttgaa
    21601 acaaaagata aatttattga tgaagaaatg tacaaaatga aaattgaaat gaacaaaaat
    21661 ttatttgatg gcaagcaaaa aattaaatca gaattggcta ctgaacttgc taaggcaaat
    21721 gataatgttt gggagaatgc gctgaaattt attcaagaaa ttcaagatga aattgaagct
    21781 aaaacaccaa tttgggctgc tgaaatcaag gagtttaata gtttagaaga acaagaacat
    21841 gaacgtatga agcaaactaa agaacttcaa ttaaaagagg aaagtattga aatcaagcgt
    21901 ttggaagctg aaatgaattt gaagcgtatg gaagaattag atcatgaaaa taaaatgctt
    21961 aaaattaaac ttgaaactgc cctttcaaaa aatgagttac tagctatgga acaagataca
    22021 aagcaagtgg tgaatggatc aaacagagtt ggcgcactta atttatttag taaacgttaa
    22081 aggagaacgg atatgagtgt ttttaataat aaagttatgt tagggaactg ttatgtgaac
    22141 cacattggtt tttacgatgg aggaacatac agagttttat tcttacgttt atctgttttt
    22201 agttactcaa aagatggaaa ggggtattat gaaaatatta atgcacaaat ttcttgtcct
    22261 ttagatagaa atggaaaacc cacgactaca agtagtcacc ctaaagcctt gatgaataaa
    22321 tttatctttg aaaagttgga tcgcaatttg gttgagggga tgaagataag tattcatgga
    22381 aatattcgtg gaacagaaca agtgcttctt aacagtggtt ggaaaaactt tggtcaatta
    22441 tcgcctttag aaaaagaaga atatcaaaaa attcctaaaa atgaattagt aacaagaaat
    22501 caaacatata tctatataga agaggtaagt atgccagaaa aagaagaatt tagaaagtct
    22561 caatctaagg taaaaaaaga agatgtggaa gttgcagaac cgaatcaagc agatgaaatt
    22621 ttatccgatg atactgatcc agtgaatgat gaaggagagg ttattgaagt ttttgaagct
    22681 gaaattatcg aagaggaaga agaagttaaa agtgttgata gaagaatgta tcgagagaaa
    22741 ccaaaaggtt atgtgaattt tgaagatatg tattatgacg aatattctaa tgaaccaccg
    22801 atcaattagt gggggtcaaa tatgaagcta gtacaaaaca atgaagtgaa agaaagaaaa
    22861 aacggctttg ttaaaaactc gaacaaaatt ttgggaaagc tgaaaaaagt aggtagtttc
    22921 aaaaaaaata aatatcatac tggaaaaatt gagggagaag atttaccgaa aagtttacaa
    22981 gataaattga atgttccgct tgtatttata ggtgtttttt taggttcaat agtttttcta
    23041 gcaataacat acttattgaa ctttttaaaa gctttgttag ttgcaatcgg ccagtcagaa
    23101 agtgggctac ttggtgtaga gttcgatcct aaatttagtt tggcatatct aaaaccactt
    23161 ggggatacga aacaatactt tatgtgtgct gtggttgcgg taataggagt cgcttattta
    23221 atatatggga aattaaatca tgaaagtgag aaaaatcttg tttacggtca aaaaggagat
    23281 tcaagacttt caactatcaa agaaattaag agagattacc atgagatacc agaaaaaaca
    23341 gaagtgtatg atggtattgg tggtgtgcca attagtcact atcaagataa atattatatt
    23401 gatagagaca cagtaaatac cgttgttttg ggagcttcac gatccggtaa aggggaaaca
    23461 tttgttgttc ctttattaga taatcttagc cgagcaaaaa tacaaagtaa tatggtcgtg
    23521 aatgatccga aaggagaatt gtttagcgct tcaaaggaaa cacttgaaaa acgaggttat
    23581 cgtgtattag tgctaaatat tgatgatcca cttgaatcaa tgagttttaa tcctttacaa
    23641 ctagtaattg atagttgggc taacaaagac tatcacgaag cgagtaagcg agcgaatact
    23701 ttaacttcaa tgctctttgc ttcaggaatg ggtacagata atgagttttt ctataaatca
    23761 gctaaatcag cggtcaatgc gattatttta acgattgtag aacactgttt taacaatgat
    23821 tgcattgaaa aaattacaat gtacaacgtg gctcaaatgc tgaacgaatt aggaagctta
    23881 ttttacactg atccaaagac tggaaaagaa aaaaatgctt tagatgaata tttcaatact
    23941 ttaccacaag ggaatcaggc caagattcag tatggttcta cttcatttgc cggtgataaa
    24001 gccaaaggtt cgattttatc tactgcttca caaggtattg aaatgtttac aagtgatttg
    24061 ttcgggaagt taacaagtaa aatgtctatt gatttgaaag agataggctt tcctaagtcc
    24121 atacaattta aagtcaatac gaatttagtt ggaaagagga ttagtatttc atttttgaga
    24181 aaagaaaatg atgtaattag attaattaag aagtatcgag tgaaagtaaa agctttaggg
    24241 ctatgtgttt taaattttaa tgaatatctt caggatggag atatgttgga tatacgatat
    24301 gaagaagaga aatcgagagc atggtatcgt attgaatttc ccaaaaaaca agcaaatagt
    24361 catgatactt ctttagtcac taaaaaattc aagtcaggta aaaattattc tgacttagaa
    24421 atcagcacct tacgattaaa atacacggat cagccaactg ctgtgttcat ggttattcca
    24481 gattatgatc cgtcaaatca cgtacttgca agtattttta tcagtcaact ttatacagaa
    24541 ttagcaagta attgtaagca aacaccggat aaaaaatgtt ttaggcgagt tcatttcttg
    24601 ttagatgaat ttgggaatat gccggctata gacaatatgg atgggattat gacggtatgt
    24661 ctaggaagaa atatgctttt tgacttagtg attcaatcct atagtcagct agaaacaaga
    24721 tatgataaag cttttaaaac aattaaagaa aattgtcaga atcatatttt aatcatgtcg
    24781 aacgatgagg aaacgataga agagatttca aaaaaatgtg gtcataagac aacaataaat
    24841 agaagtgcta gttctaaaca tttagatact gatagctcgg tcactagttc ggcagagcaa
    24901 gagcgagtaa ttacagttga gcgtgctagt caattgattg aaggggaaca aattatttta
    24961 agaaaccttc atagacaaga tacaaaaaga aataagatta gaccatttcc tatttttaat
    25021 actaaagaaa caaatatgcc gtatagatac caatttttag cagaagactt tgatactcaa
    25081 agagatatta atgagattga tattgcatgt gaacatatca atctatcttt acaagataac
    25141 caaattccat atgcaaggtt tatcaaagat ttaaaaacac gtttggagta ttcaattatt
    25201 aataatattc ctattagtga agaagactat caggattatc taaatttatc aggatcacaa
    25261 caaacaatta atgaagaaga gctgttaaaa ctggtaaata cggaagtaaa aagtcaagat
    25321 atggaaggtc aagttttatc agaacaagaa atgaaaatta atatttatct aaaggaaagt
    25381 caaaaggaag caatagaatt agtaaaagta attaatggaa ggttagctga tgaaaattca
    25441 gcagatatgg ttataaaaac cattaattca caaatccttg atacgccttt cattcaatct
    25501 ttaatcaatg attctatagc tattcataat gttcaagaga gtaaggatca aattatgatc
    25561 ctagaacaac aattagctaa tgcttatgag ttagcagtgt ttaaaacgaa aaattcaata
    25621 ctgaaaaata aaattaccaa gttgaaaaat gctatgacta agctagcaaa agcaatttag
    25681 agagaaggga agaaatattg actgaatcta ttaattggag aaaactaaat gtgcaatggt
    25741 taaagtataa atggccaatc atagtttccc taataatcat tctatttatg gcctatccgc
    25801 aacgagtttt tgcgttgccg tggattacag acaataaaga ggtattaaaa tttttaacaa
    25861 aatatcaaga ttatcttcag tttactaaag gtggttttgg aagcattgtc gttcaaagtt
    25921 tatcgtgggg gctgattaga ggtgcatatt ttgtttgtaa atcagcagaa aaccttgttc
    25981 cacaatcgct aagtttgttt agctttgttg aaagcagtag tttaggtgct gtttatcaat
    26041 ctgtattaaa cacggtggtt gtaggattaa tgatactttc attgatgttt gttggttaca
    26101 agatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt
    26161 ctatagcatt aatactttta atgccaacca tgatttctag tggtattcaa tttgctaaaa
    26221 cattttataa ggactctact acgattacaa aagctgatgg gggcgttgct tggtcattga
    26281 ttgaggacgg tgtaactgat ttagtatata tcaataaaaa tgatgcttat gatcggttgg
    26341 gtaaagatgg cgtacaaaaa aatgcgttaa aagaaaagaa tttcttttcc tatgatttca
    26401 gtgaagtaat cacagttgat acagctaaag atttagaaga tgataataaa aaagctgaat
    26461 atcttcaatc taggttgaca ccagatggcg aaggaaatat gcaagccgtt aagattgatg
    26521 atggttggat gtcgatgttc tctgatagtt tcaaaggagg gtactacagg tttactaaaa
    26581 atacttttgc attgacttta ggattacttt cgttagctgt agcctatgtt tttagtattt
    26641 ttatcatagt caccgctatt ttagaactgg catttaagcg tgtgttaggg gttcttgttt
    26701 ttgcaacaga tattgagaca ggacagcgtt caaaagttgt tctatcagat attctacagt
    26761 gctatttgac tgtaggcttt caaggatttg gattatccat gtttgcaatg tttgtttcct
    26821 atttaggaac cggcggaggg tcttccgtta atccgtttat taaggctgta gcttatgtat
    26881 gtgccgtttt tgttttaatc aaaggcagtg gaacagtcat gagatacttt ggtgttgata
    26941 ttggactcaa agaaggctat gggcaacttg cttctgcttt tggaatgggt gcaatgctgt
    27001 ttagaaaagg atcaaatgga tttaatcgtg ccaaaggaag cggaaacgga aatggatcag
    27061 acagcggaga aggagaagat cgaaaaccag aaaagaattt cggtgaaact ctatcaaaaa
    27121 aagctggcaa aactggaaga gctttgggat atgcccatga acgtggatta tcaggtttag
    27181 cttcagatgg agctacaatg gctagtgaaa gagcaa
//
LOCUS       DANKHJ010000033        25589 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000033 DANKHJ010000000
VERSION     DANKHJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            127..3654
                     /locus_tag="PW113_001530"
     CDS             127..3654
                     /locus_tag="PW113_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2525847.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="PW113_001531"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="PW113_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2525848.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="PW113_001532"
     CDS             7442..7783
                     /locus_tag="PW113_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525849.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="PW113_001533"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="PW113_001533"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2525850.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="PW113_001534"
     CDS             9140..11560
                     /locus_tag="PW113_001534"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2525851.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PW113_001535"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PW113_001535"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2525852.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="PW113_001536"
     CDS             12994..13731
                     /locus_tag="PW113_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2525853.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="PW113_001537"
     CDS             13745..14560
                     /locus_tag="PW113_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2525854.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="PW113_001538"
     CDS             14573..15220
                     /locus_tag="PW113_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2525855.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="PW113_001539"
     CDS             15236..15892
                     /locus_tag="PW113_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2525856.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="PW113_001540"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="PW113_001540"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2525857.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="PW113_001541"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="PW113_001541"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2525858.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="PW113_001542"
     CDS             18234..18752
                     /locus_tag="PW113_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2525859.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="PW113_001543"
     CDS             18859..19359
                     /locus_tag="PW113_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2525860.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="PW113_001544"
     CDS             complement(19639..20559)
                     /locus_tag="PW113_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2525861.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="PW113_001545"
     CDS             20724..21737
                     /locus_tag="PW113_001545"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2525862.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="PW113_001546"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="PW113_001546"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2525863.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="PW113_001547"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="PW113_001547"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2525864.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="PW113_001548"
     CDS             complement(23377..24138)
                     /locus_tag="PW113_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2525865.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="PW113_001549"
     CDS             24327..25421
                     /locus_tag="PW113_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2525866.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8477 a         4435 c         5397 g         7280 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttg
//
LOCUS       DANKHJ010000034        24759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000034 DANKHJ010000000
VERSION     DANKHJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..800
                     /locus_tag="PW113_001550"
     CDS             <1..800
                     /locus_tag="PW113_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525867.1"
                     /translation="NTDEKEALTKERIQEAKNSNTGLEKLVKENLQNSTSSQGGRSVD
                     VRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDVRENLQSSSTSQPKTVDVRENV
                     QNSTSSQGFKSVDVRENVQGSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSA
                     SQPKTVDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDS
                     NHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLN"
     gene            824..1159
                     /locus_tag="PW113_001551"
     CDS             824..1159
                     /locus_tag="PW113_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525868.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            1156..1773
                     /locus_tag="PW113_001552"
     CDS             1156..1773
                     /locus_tag="PW113_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525869.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            1790..2257
                     /locus_tag="PW113_001553"
     CDS             1790..2257
                     /locus_tag="PW113_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525870.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRSRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEENSNSKSAFFSEKNAFLHSI"
     gene            2273..4228
                     /locus_tag="PW113_001554"
     CDS             2273..4228
                     /locus_tag="PW113_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HDL2525871.1"
                     /translation="MIVSKKIARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIEDQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDECHNVINSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPK
                     DSGSEVADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAV
                     VQTSSEDTYMINFDPYPEQIERFDGGQ"
     gene            4250..5386
                     /locus_tag="PW113_001555"
     CDS             4250..5386
                     /locus_tag="PW113_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2525872.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            5401..6051
                     /locus_tag="PW113_001556"
     CDS             5401..6051
                     /locus_tag="PW113_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2525873.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPNDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            6065..6961
                     /locus_tag="PW113_001557"
     CDS             6065..6961
                     /locus_tag="PW113_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525874.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMMIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKNDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            6997..7227
                     /locus_tag="PW113_001558"
     CDS             6997..7227
                     /locus_tag="PW113_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525875.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            7578..7874
                     /locus_tag="PW113_001559"
     CDS             7578..7874
                     /locus_tag="PW113_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525876.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVKKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISGRSDLNK"
     gene            7871..8338
                     /locus_tag="PW113_001560"
     CDS             7871..8338
                     /locus_tag="PW113_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525877.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKANMFRYVVSNLP
                     MLTKANQNFRDPEKEELTKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEEHVEPEKESSRLGDGTMFSSPSLRFTTD"
     gene            8406..9941
                     /locus_tag="PW113_001561"
     CDS             8406..9941
                     /locus_tag="PW113_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525878.1"
                     /translation="MVGDEMTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELVRIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENILYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFNREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            9963..10292
                     /locus_tag="PW113_001562"
     CDS             9963..10292
                     /locus_tag="PW113_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525879.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDNVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            10294..10557
                     /locus_tag="PW113_001563"
     CDS             10294..10557
                     /locus_tag="PW113_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525880.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKIALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            10863..11840
                     /locus_tag="PW113_001564"
     CDS             10863..11840
                     /locus_tag="PW113_001564"
                     /inference="COORDINATES: protein motif:HMM:NF022432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDL2525881.1"
                     /translation="MEDKKYSKIIKKIKGLLAIAKDENQDEESQSAFVLAQRLMLQYK
                     IDKNDVFDTEDEIEPIGEESVTIYKKLYWWERSLGSIIAENFRVKMFYNSKKEKGERK
                     SKSKIVFYGFGSDLELAKEMYFLAYEVIVFHTNQYIKNWYQDTDMKRSRYITESVKSS
                     YISGFLVGLQKRFEEQISVLAERYEVMVLIPKAVEESFDKYSKDFSSYSTNTPPVSVD
                     HAYQEGVREAKKIDLTKSTVDTDYSSLVGRIIRFNQGVTRGLIAEITSVSDDLLNLLV
                     MNCVSEYASEDNPSFYHWKVDKEYDYEILSFNDPMSKKFKKNKVDIMEN"
     gene            11992..12858
                     /locus_tag="PW113_001565"
     CDS             11992..12858
                     /locus_tag="PW113_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525882.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEINGVQYSEPEWEKVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYEPTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            13013..14839
                     /locus_tag="PW113_001566"
     CDS             13013..14839
                     /locus_tag="PW113_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2525883.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSIPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            14847..15920
                     /locus_tag="PW113_001567"
     CDS             14847..15920
                     /locus_tag="PW113_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525884.1"
                     /translation="MSNEQNYETDGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            15929..16189
                     /locus_tag="PW113_001568"
     CDS             15929..16189
                     /locus_tag="PW113_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525885.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQVAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            16226..17230
                     /locus_tag="PW113_001569"
     CDS             16226..17230
                     /locus_tag="PW113_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2525886.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            17255..17611
                     /locus_tag="PW113_001570"
     CDS             17255..17611
                     /locus_tag="PW113_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525887.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREYDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            17773..17964
                     /locus_tag="PW113_001571"
     CDS             17773..17964
                     /locus_tag="PW113_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525888.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            18073..18291
                     /locus_tag="PW113_001572"
     CDS             18073..18291
                     /locus_tag="PW113_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525889.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            18328..18645
                     /locus_tag="PW113_001573"
     CDS             18328..18645
                     /locus_tag="PW113_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525890.1"
                     /translation="MKDYALRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRLIEVNNLGIIEDYLAKLDEAVKQSIEKNGEDHVLTVVLKDLKDRLEAEAENF
                     NKD"
     gene            18656..18853
                     /locus_tag="PW113_001574"
     CDS             18656..18853
                     /locus_tag="PW113_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525891.1"
                     /translation="MRDMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            18850..19818
                     /locus_tag="PW113_001575"
     CDS             18850..19818
                     /locus_tag="PW113_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2525892.1"
                     /translation="MSLGNEKPYYVVIGGVNGAGKSTIYQIQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVIREIKDHFKEGKSFHHETAFVGNEYPHKKRLLNAKK
                     LGYESFLIYVGLDSPEKAIERVKSRVAKGGHGVGEDLIRERYVSSLRNLAGLEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYNWVPKAIQEKRAEIPISKQIIKDISQEGL
                     SPKETLERIYSESDDFISDRFFKAEQVDDFYMKHKNEIKERVLAISFSTGVSEDELVG
                     QDKKRNSCKLAYINSVGELLEKTDSELSSMEASRDRSIKKTDFEIE"
     gene            20317..20880
                     /locus_tag="PW113_001576"
     CDS             20317..20880
                     /locus_tag="PW113_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525893.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFHKRSD
                     CMKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERGTNLPNPQKLKLLS
                     TITNSTIDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSKEFEVNQIP"
     gene            20894..21676
                     /locus_tag="PW113_001577"
     CDS             20894..21676
                     /locus_tag="PW113_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525894.1"
                     /translation="MKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERG
                     FSLPNKERLIKIAELSNKTIDWIKWGTLEEYIASYLKDSGYELYIKDFPEIPHKVFND
                     IQERYSDTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGCEVSNSPRSTVSTWEHGGNLPNKAKLRKIADIANCSVEDLLFDEGFIF
                     KQSKVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            21716..22327
                     /locus_tag="PW113_001578"
     CDS             21716..22327
                     /locus_tag="PW113_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2525895.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            22487..22657
                     /locus_tag="PW113_001579"
     CDS             22487..22657
                     /locus_tag="PW113_001579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525896.1"
                     /translation="MNQQVNWVNDFEKEFRLKFFGNPTAESTEEKNVVYFENTRNTER
                     AVKIDQNIKVRI"
     gene            22752..22901
                     /locus_tag="PW113_001580"
     CDS             22752..22901
                     /locus_tag="PW113_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525897.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFD"
     gene            23556..24119
                     /locus_tag="PW113_001581"
     CDS             23556..24119
                     /locus_tag="PW113_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525898.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            24106..>24759
                     /locus_tag="PW113_001582"
     CDS             24106..>24759
                     /locus_tag="PW113_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDL2525899.1"
                     /translation="MKRFKGSEMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     "
BASE COUNT         9862 a         2897 c         4747 g         7253 t
ORIGIN      
        1 ctaatacgga cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa
       61 ataccggatt agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg
      121 gtcgatcagt agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga
      181 atgttgatgt aagagagaat ttacaaagtt cttcaaatgg tcaaggtact aagacaattg
      241 atgttcgaga aaatctgcaa agtagcagta caagtcaacc aaaaacagta gatgttcgag
      301 aaaatgtaca aaattctaca agtagtcaag ggtttaaatc agtggatgtt cgagagaatg
      361 tgcaaggtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt
      421 ctacaagtag tcaagggttt aaatcagtgg atgttcgaga gaatgtgcaa ggtagcagtg
      481 caagtcaacc aaaaacagta gatgtccgag aaaatgtaca aagttctaca agtagtcaag
      541 gattcaaaac agtagatgtt cgggagaacg ttcaaaaaga tagtggagaa atgaaagaaa
      601 aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc gaaaaaattt gattcaaatc
      661 atgaaacagt gattattgat agtgaaatcc gtgaaaatga taagggatca aaaccaagac
      721 gaagattcac gtatgaagac aacgaattat tcagagatac acttaatgat cctaatccac
      781 ttttcgataa tttaaattaa attaaaagcc ggcatgagtg attatgccgg ctttttatga
      841 ggtgaaaatg atgtatagaa atcccaaaaa tataaaacaa gagataaagc tatttttctt
      901 ctatttagtc gatttaggaa tagtagccgg aagcgtgatg gcaagtgttt actttgcaaa
      961 attaatgcca atttcaccaa tgatgaaatt tgcactgtac ttattagcat ttgtagtggg
     1021 tgtgtggatg tgcgttagaa cgcctaatca ccctaaagag cgaaatattt taatgctgta
     1081 ttatctcttt aaaatggata gaaacagata ccacgaagta gagtttaaaa gaaatagtag
     1141 ggggaaaatt gaattatgat tgttgctgaa agtaatgaaa ataaattaag tggtgttttt
     1201 gacaaattct tagaactagt tattccaaaa gataaagata tgtgtgaact tttaccatat
     1261 aaaaaaatat tgaaagacgg aacgttagtg actaatgaca ataagtatca agcctttttt
     1321 aaagttaaaa caactgactt aacttctatt tcagaagatg gatttatgcg tatggtaaac
     1381 aaactcactt atttaaatcg agtatattta gaaccatata aaatttattc attaacttat
     1441 cctactgaaa cagtaaatca agcgatttat tggaagcata gaatacagga ggcaaagaga
     1501 agtttattga gtaataatga tcccaattta gaaacgtatc tgaagtggaa tctaaagcta
     1561 gcttacgata attatgaaag agttgatcta gtatcgactt taccagagtt gatgttcgtt
     1621 atttgtgttt atggaaaaac acaaaaagaa gtgaaaaatc atgccgatgt aatgaaacag
     1681 gccggtggta gagaattaga tttacaaaga attactgaag gggatttaat caatgtgata
     1741 actaaattga tgaatcccaa tacttcttta taaggcggtg cttattatta tgttaaagca
     1801 ctgtagaaaa ggattttgta aagtaattaa aggtaaaaat gctgctgatt taatctttga
     1861 atggaaagaa tctaatttaa ttaatgtacg tgggaaaaat ggaagcggaa agacagaaag
     1921 agtgatggct agtattgata attcaaggtc tagatcaaga gcattagtaa taactgatga
     1981 aaaaaatcac tataaaggca tacgaacgat agaaaaaaaa tatttaacta acaatgattt
     2041 aaaagtagta gatgatattt taaatattta tcctgatttt aatttagttg tttttgatga
     2101 aacaattcct gtgacagaaa taaaaggatt ctatcaaagc ttttccaatc tagcaaataa
     2161 atatgaggaa gttctgtttg tttggattac agaagaaaat agtaatagca agtcagcgtt
     2221 ctttagtgaa aagaacgctt ttttacattc tatttaaaaa ggagaattga acatgattgt
     2281 aagtaaaaaa atagcaagaa atctagtaag tgaagggtat gacttaagat tagtagaaaa
     2341 gtatcaaccg agcggtggaa ttaaatttga tgagcgttat atttctactg gtgatggata
     2401 tgtaacttcg attgctgtta ttgagtttgc agaagatgta aatagtcgat ggttaacgga
     2461 tataatggca attccaaata ctattgcaac tttcgatgta tgtacggcgg ataaagaaga
     2521 cttattacac aaaatcaata cgtctattgg agaattagat taccgatcaa caaatgagaa
     2581 tagaacaact gacaaaacag acgctttact agataaatac aatttagaag attttgcaca
     2641 agaaattacg aaacgtggtg aagttgtcaa aactgttcat gctcgtattt ttgtttatag
     2701 cgattcttta gaagaattag agaaaagaat tagtgagatt cgtaaagatt tggctagtgt
     2761 caatcacaag gcaattgttc cgcaatttag aatgaaggat gaatggatgt ctttatttca
     2821 agatggcatg gaacaagaat caaaattatt tgccaagaaa ggcttaactg ttccggcaaa
     2881 aaatattggt ggcggtattc catttcacca caagagtttg aaagatccac gtgggtttta
     2941 tttaggaact acgtcaactg gtggtgcttt tatatttgat ccattttatt ctacgaagca
     3001 aagacggtcg tttaacggct ttattttagg aaagatgggt tttggtaagt caactttaat
     3061 taaacagcta caggaaggcc tacaagcgaa aaattgtttt attcgaggat ttgacaagtc
     3121 aggtgaattt gaaggcatgg tgaagaaagc aggcggtgta tacgtcgatt taggcggtgg
     3181 tgatatggta gtaaatccat tgcaaattta tgcaacagtc atgaaaaaag atcaatttgg
     3241 aaatgatgtt gtagatgaat caaagagttt tcaaaatcat atcactaagt gtagtaatca
     3301 aattcgcttt ttagaacctg aaattgatac ggcaactgta cgtgaatata ctttgatgtt
     3361 acaagacttt tatattcatt atggaatttt gccaaaagaa tggagagaag cgagagacga
     3421 caacgaacga aatcaattaa atacaaatat ttgtaacttg cctaatgatt catatccaat
     3481 ctatggagag tttcttaaat ttattgaaga tcaacaattc aaaattaaaa atgctactcc
     3541 tgaacgtgtc cgttctattg agctaattaa gcttatcgtt acggatatgt gtacaactta
     3601 tccgcaaatt ttcaacggag taacaacaat gccaaacttt gatacaacaa aaattgttta
     3661 ctataacatt gattctatta gtaaaatgag tgccccagta ttccattgcc aattatttac
     3721 agcgttaacg actatttgga acagtgcctt gcaaaacgga aagattcaaa aagaaaactt
     3781 taaaaaatat ggcagtgatt attataagta tgcacaagat ttcatggttt tgatcgatga
     3841 gtgtcacaac gttatcaatt caaataacaa gtatgcttgt gaatatgtac taagttttga
     3901 gcgagaaatg cgaaaaatgc gtgcaggtgt ttattttgca actcaaacgc ctgaagaaat
     3961 tatccctaaa gattctggat cagaagtagc agatgtgatt agaaacattt ttaatctatg
     4021 tacttttaaa tgcttcttta atttagattc tgcattgttg gaagacataa aaaaagtttt
     4081 aggaaataca attactgata cagaaattat gcttttacca gaattagaag taggacaagc
     4141 tgtagttcaa acttccagtg aagacaccta tatgattaat tttgatccat atccagagca
     4201 aattgaacga ttcgatggtg gtcaataata gaaatcggag gtgaatttaa tggagcagaa
     4261 caaaaataat aacgttgctt caacactcgc tcgtcaaggg atgaagcaag tatcaaagaa
     4321 aataggtctt gctttaaaaa taaaaatagg ggtagttata ggactgataa cattggttgg
     4381 ccttttagtt ataggttctt tagctacatt acaaggaaca caaactgcaa ctaatacagg
     4441 aggtgcagaa gtcattggtg gcaacaaacc gttgtcacct gaagttcaaa aatggcaaaa
     4501 aacggtagaa aaatatgctg aaaaatacgg aattaaagaa ttagtacctt atgctttagc
     4561 aattatgcaa tgtgaatccg gaggtaaatt gccggattta atgcaaagta gcgaatcagc
     4621 cggattgcct gtaaacacac ttcaatatga agaaagtatc gatcaagggc taaaacattt
     4681 gagtaatgtg tataacattg ctaaaagtta tggcatggaa agtaactatg atggaatagt
     4741 tcaagcttac aattacggaa tagcttacat gcactactta ggaaaaaaca aaaaaacaca
     4801 tactatcgaa acggctgaag cctactctaa aaatgttgtt gctccaagtt taggaaatac
     4861 tacaggacag acttattcat atgtaaatga agtatcaatt aaatatggaa aaacatattt
     4921 gtatttaaat ggtggtaatt tttactatgc agagttagtt aaacagtata tcggtggggg
     4981 tagtggtaat ccggctaatc cttcagaatt ttgggggaaa atgaaaacag aaatggaaaa
     5041 atatcaaggt tggccttatg tttggggcgg taaaaatcca acgacaggtt ttgattgtag
     5101 cggattaaca gcttatcttt atcaatttag cgccggaatt acaatagata gttatacagt
     5161 aacgcagtat gaagcaactg aagcagtatc aattaaagaa gcaaaagctg gtgatctgat
     5221 tttctttaga ggaacatatg gagcgcctga tttcatttct cacgtaggta tttatgtcaa
     5281 tgaaacaact atgtatgaca gtaattcaag cggaattggt tatcacaatt ggcaagattc
     5341 atattggcaa tcacatagtc cagagattcg tagagtcaaa aaataggagg aagaaaatag
     5401 atgattaaga gaaaaaatga taataaatgg aaatatattt gtggtgtttt aattattatt
     5461 ttagttttag gtggattgtt atctcaactt catacaagta acattcaagc aaaaaataaa
     5521 aaattggatc aagaagttac acaactagaa gaacaaaata gcaagttaag taaaaatatt
     5581 agtgatctaa atgcaaatgt agaagcgaac ttaactaatg atgatggcag tgaattaaat
     5641 aaacaaattg ctgaagttgc taagaaattt gttgagctat atccggtata tgatattgaa
     5701 aaagtagaag aaaagaaagc tgatttagag aaaatagcta ctccttctgt agctgattct
     5761 atcgtgccga acgacatgat tacaacgagt aaaaagactt tagaaagtaa atctactact
     5821 caaaaaggag aagcatattc aagtgatcca acattcaaaa gtaaatataa agattcaaaa
     5881 ttatatacga attatgttaa cgccaatact attaattatt ttgctgaagt aaattataaa
     5941 acagaatctt ctagtggaga cacagaaaat acagtctata tgctttttga agtaaccaat
     6001 aaagacggcc aagtgttggt agataattat gaaatcaagt acttaaaata gtgaggtgaa
     6061 gtatatgaat aagaaatatt ccattcataa taagaaggaa ccaaataaaa aaaataaggc
     6121 acaaattgac aaagtatata aaaaaacgaa aggagtaaaa ttatatttag gtgctatact
     6181 tggattttta gcaataggaa tgatgatcgt tttttctcct tatgtgatgg ggaaaagtta
     6241 tgattttgaa actgtaaaaa tgagttcagc ccaaaatgta acgtcttctt tgaatatgta
     6301 tgttgatgat atggaaataa atagggaagc cggactgttt aaattagtgt tgcggtatca
     6361 agatgataca ggtactaaat cactaagtaa tattaaaaat gactataaat taaattttat
     6421 aacgaacaaa aataatgaag atgttaagac aaaagttgta aaattgtcag atgaatacac
     6481 ggtaatttat tatcaaaatc tgcctaaaga ttttggtgta atttctgtga cagttaaccc
     6541 tagatatatt tatcctgaat tagaaccttc aaatgactta aaagaaaaag aaataaaatt
     6601 ctatgcagta gacaaagata ttaaatcaaa tgctaaattg gaagttgaaa gtacttctga
     6661 tttgcaaaaa gataatttta aatatcagat taaagctata gaaaattcta taaaagatga
     6721 gaaaaaatca attgaaaaaa tgaatttagc aaataaagta gtgcaaaaag atattgataa
     6781 agctaatcaa aacttagaat ttcaaaccct agacgaacaa acagatacaa aaaatgaaat
     6841 cagttctatg cagacgacta ttgaaaataa taaatcttca atagaggaaa gtgaaaataa
     6901 aatcaaacaa tttgaggaaa aaataaagca actagaaaag tctgcaagcc gatttgagtg
     6961 atcgtgttta aattatttta agggagttta gagagaatgt gtaattctat tgtcactatt
     7021 caagagaaag ctgaagaaga attaatgaga attgaagcgg aatataaaga aaagagaaaa
     7081 gcaattaagc gcaaaaaaaa tgcagagctt aaaaaatata aagaggaaca agcgttaaaa
     7141 ttaggaatga tgttgtgtaa atattatgat tgctatgata taaacgaagt gctaaaaaaa
     7201 gcgaatttaa accaattttg tgaataggaa agagagtggc aaggtaaaac ttgccactct
     7261 ttttttgggc gtaaaagctt tagttactat gaccaaaact acggacgaaa ctatgaccaa
     7321 aagtaggtac tatattatga ccgaaaatag gtcgaaaatg gatttttttg tgatagcaaa
     7381 aaatatgaag tgattttgaa agaaaaccag ttggcacagc caacttagcc accttcctaa
     7441 aggaaggtgc tatcagtttt cccttatgct ctttgtatta attctttggt ttcgtcttta
     7501 aatctttaat tctcctttgg actttgatat aataatgtat accagataga taaaaactct
     7561 ttgaaagagg tgtatatatg gatgtttcaa ttagagggat tgatccacaa tatgtaaaag
     7621 agattgataa aaaagttaaa aaaattcaag aacgtaccgg aaaaaagttt agtaggaatg
     7681 actatttttt agcaatcatt cgtcaagata gtgagattga tttgattgat tataaaaagt
     7741 cagaatttga tttagtagtt gaaaaacttt tattgtcaaa tgagcaaatg gtagaaagta
     7801 ttagtgagtt aacttcagtt tatgaacgct tattcaattt tttaattagc ggaaggagtg
     7861 atctaaataa atgaactacc cttatttcaa agtatcagca acagaagatg taaaagaaaa
     7921 attcaataag tgctttgaag taaacaagtc agtatttcaa acaaaagcta atatgttccg
     7981 atatgttgtt tcaaatttac caatgttaac caaagccaat caaaatttcc gtgatccaga
     8041 aaaagaagag ttaactaaaa acttagaaga agcaaaatta aaaattagcg aacttgaaac
     8101 gataatagaa ttgcaaaaac agcaacttga aaactttaat aaaaaagaag acagtacaca
     8161 caaagattat agtgaacaat tgaatagtat agaagagtct gtctataagc tattaattaa
     8221 tagtggatta gctagtaaag aattagaaga agagcatgtt gaaccagaaa aagaaagtag
     8281 tcgtttagga gatggaacaa tgtttagttc accgtcatta agatttacaa cggattgaac
     8341 actaaaaaat tctaatgcaa cttagcatta agtacaaaac cgcctgtaga aaaaaatttt
     8401 tgaaaatggt gggtgatgaa atgactgaac atatctttaa cagaaaaagt gcgagcatta
     8461 ttcttaaagc aaaatttgaa accggaaaaa acaaaaagaa gaatcacatg gtagaacttc
     8521 aaaaatttgt agattacatt tccagacaag aggctattag gcaagataaa ttagatcatg
     8581 attattctga agatgaacta aatgaattag tcagaataga aaaagctttg gaaaaaatag
     8641 aacaggaaac agatcaacca gtgattaaag atttgcgtga aatggataaa tatattgatt
     8701 atatgacacg taaaaaagct attttagaaa atgtagataa tgaaatagta aacggagctt
     8761 ttagtaatac aaaaagatat atcactaaaa aagacatttc aaaaataaaa gaatcggtta
     8821 tagaagcaaa aaataatgga tcggtgatgt tccaagatgt aatttcattt gacaatgatt
     8881 ttttagtaag ggaaggatat tataatccag aaacaaatga attaaatgaa aatattttat
     8941 atgaagcaac aaagtctatg atgggaaaaa tgcaagaaaa ggaagaacta gttgatcctt
     9001 tttggtttgc aacgattcac agaaatactg aacatatcca tattcatgta acagctatgg
     9061 agcgtaaaaa tactcgtgaa ataatggaat atgatggtgt tttacaagct aggggcaaga
     9121 gaaaacagtc tacgttagac gatatgattt ttaaattcgg tagcaaaata ttagatagaa
     9181 cgaacgaatt tgaaaaaatt tctaagttga gaaaagaagt tcctttagaa cttaaacagt
     9241 ctgtaaaaga ttctctgatt caattatatg tagaaaataa ttcaagatat gacaaagaat
     9301 tagtaaagta cctaaaggaa ttgaaaaaag aaatacctag tacaacacgt ggctacaatg
     9361 agcttcctga agaaacaaaa gaaaagatcg atgaagtcac aaattatatg acaaaggaca
     9421 atccaaaaag aaaagaatat gatcgtatga ctaaagaaat tgatgagcta tatcaaacaa
     9481 cctacggtaa acgctacaat ccacaagaat attatttaaa tagaaaaaaa gattttaatg
     9541 ctcgaatggg taatgctgta gtaggacaaa tcaaactttt aaagcaatct gaagaaaaaa
     9601 atttgaaaag taatgaactt aaaaatctat tagacggaaa aaaattaaac gttaagttta
     9661 atagagaaaa accagaattt aaaacgaaat atgctaaaca aagttatgat gatttccaaa
     9721 aaagattaga agaacgacaa ttagcacgtg aaaaaaatga agtccacttc aagaagattt
     9781 ctgaaaaatt taaagataaa aagcttatta gaaaaattga tagagtaatc aatgatgatt
     9841 taaatatgta tcgtgcgaaa aatgattatg aagtagcgca acgaatgata gagcaagaga
     9901 aaatgagaca agcacgtgaa tttgaaatag gaatgaatta gtagaaacgg agaacaaaaa
     9961 ttatgaaaaa cttcgatata gtgtgcagta atacaaaaaa catatattta cgtgagctac
    10021 ttaacagtga ttcggaaact atagaagatg taaaaaaaat catagtgttg tttgaaaaag
    10081 aaaacatgga gctagagaat tgggggcttt ttgaaatacc gattagtggg aattactgtt
    10141 tttataattg gaagacagaa gacaatgtag cttttgcaaa ttactttttt gataaaaatt
    10201 acttttcccc attatatata gataagcata gtaatgaaca agttgcttct tcaataaaag
    10261 aagcaataaa attggaaaga gtgaggaaat gatatgatcg attttagaac ggttatggat
    10321 aaactaagta atggagattt tcgcgtattg attgaactat acttaaatgt aacgtctaca
    10381 gtcaaagagt ttttatattc taatcccaaa atagctttag tattagttgc agtactgata
    10441 gtttttgctg taatttcttt tattaagaaa atgattccgc tattaattat aggtgctgtg
    10501 ttgatattag gcttgtattt ccctgaagtt ttggatcaaa taaaaaatat attttaggct
    10561 ttaaaagctt ccagtagaaa attttaggtc ttgacgttcg ttttaagcgg ttcaaaatag
    10621 aaatttgata aatcatacga agagacatta tatggtcgtg tagagcgaat taggaggctt
    10681 aaacattttt gaaaagcact tgactacatg tagtcaagtg tgctaagcta tttttgtagt
    10741 aataaaatat aaggaattag aggaagttag tgtacttcct ttaatttaag ctcgaaagac
    10801 tacaagtagt ctttgggttt tgagaggtaa aaatatataa ttaaacttgg taggtgaaga
    10861 aaatggaaga caagaagtat tcaaaaatta taaagaaaat aaaaggtctt ttagcaatag
    10921 cgaaagatga gaatcaggat gaagagagtc aatctgcttt cgttttagca caaaggctta
    10981 tgttgcaata taaaatagat aaaaatgatg tattcgatac tgaagatgaa atagaaccaa
    11041 tcggtgaaga gtcagtaacg atctataaga aactatattg gtgggaacga tcgctaggat
    11101 caataatagc cgagaatttt agggttaaaa tgttttacaa tagtaaaaag gaaaaaggag
    11161 agaggaaaag taaaagtaaa atagtatttt atggattcgg aagtgattta gagttagcta
    11221 aggaaatgta ttttttagcg tatgaagtta ttgtattcca tactaatcaa tatataaaaa
    11281 actggtatca agatactgat atgaaaagaa gtagatatat aacagaaagt gtgaaatcaa
    11341 gctatatatc cggattttta gtaggtctgc aaaaacgatt cgaggaacaa atttctgttt
    11401 tggccgaacg gtatgaagta atggttttga ttccaaaagc tgtagaagaa tcctttgata
    11461 aatatagtaa agatttttct agttatagta ctaatactcc accggtatct gtggatcatg
    11521 cgtaccaaga gggagtaaga gaagctaaaa aaatagattt aacaaaaagc actgttgata
    11581 cagattatag tagtttagta ggtcgtatta ttaggtttaa tcaaggagta actcgtggat
    11641 taatagctga aatcactagt gtttctgacg atctattaaa tttgttggta atgaattgtg
    11701 tttcagaata tgcttctgaa gataaccctt ctttctatca ttggaaagta gataaggagt
    11761 acgactatga aatcctttct ttcaacgatc ccatgagtaa aaagtttaaa aaaaataaag
    11821 tagacataat ggaaaactaa aaaaagtgat aaacaaagga ctgattattt ggttctttgt
    11881 tttttgctat atttttctac aggcaattat cttaagagta tctgtgtatt agaataaaaa
    11941 tatgataata tgaaattatg tatcagaccg ataaaaaaga ggtgcttata aatgaaaaag
    12001 ttaagagtta ttgcaacaat acctatgatt tttagttttg cgctattatt atctgcatgt
    12061 ggtaaagaag aagctggtgc taagtcaaat gaagcacata atatctcatt catagatagt
    12121 gcagaacaaa aagattcacg ttgttggttg gtagtcgaaa caagtagtaa aaataaaacg
    12181 cctattgata agaacgaata tgtaaaagcg gtgatccaaa ctaaagatgg caaagcaaaa
    12241 gtatttaaaa cggatatgga acagcttaca ttggaaaaag tttctaaaat gagtgatgaa
    12301 gaaataatag aacaagcatt gaacgctgat aaacaaaagt ttaatgataa gaaaaaaagc
    12361 gatattgata gctacaatgc tactttagat tctgtaaaaa cagaattaga aaatgaggaa
    12421 aaacaagagt ttcaaaatga ccaaaaaatt gctgattttc aagatagtat acctaaaata
    12481 caggcggata tagaagagat caatggtgtt caatattcag aaccggagtg ggaaaaggta
    12541 tcagcaacaa ttaaaatgga taatgaaaac aaatcacctt caatggaaac cgttaaatta
    12601 tcttataaat atttagatag tccaatttgg aatttaaata gttttggtga agtttcgtct
    12661 gaaggagaat tatcttatga acctactagt atggatttga agttgtcaat tccagttgcc
    12721 acaacgaaag ttttagataa tttttattcg ggatattcta atgatgaatt ggatcaactt
    12781 ttgcttacta aagtagcttc taaagataac aaagttcaat tagatagtaa aggtaaaaac
    12841 gtaagtatag ttgagtagat ggtaaaataa gaagggataa gatggttact ataactatct
    12901 tatctctttt ttatttgcat taagtcagtt aaattagata gaagagtttt tatatatctg
    12961 gtataattta attaggtaat ttaacttacc taaaaaagaa aggagataga agatggccta
    13021 taaaaaagga aactggaaac cgaaaacgaa agagcaaaaa caaaaagagc tagacgattt
    13081 aattaaggct tccaacagca aaatcgagca atatcagtct aaccctgaag atatgatcga
    13141 gtttgccaag ttcatgtcta aaatacacaa ttacagtcct ttaaacctat cgttgatcga
    13201 tgagggattt gaaggagcaa ttgctgtagc aagttatgat ggttggaaaa aactaggatt
    13261 tcaagttaaa gcgaaagaaa aagggatagg catttatgcg catgctccgg taacggtttt
    13321 tattgatgaa aatggtgagg aaaagacgtt aaaagaagcg acaagcgagc aaaaagaaaa
    13381 aatcaaaaat ggagaattaa gaagcaaaaa gatcaagcat tttaagaagg gttacgtatt
    13441 tgatatttcc caaactaatg caactgaagc tgatttgcca aaaatatttc ctaacagggt
    13501 atggaatttt aatattgatg gtgaattaag tgttagtcaa ttagaaaaag gtgtaaatca
    13561 tttggccgaa tctatgaata tatctataaa agatatgaat gagtctggta gaggtgaaat
    13621 gggaagcgct agaggtgaat atattcaata tgttgatggt agggaagaaa ttgtattaaa
    13681 ttcaagaaat agcagaacac aaaatcttac taccagtatt cacgaattgg ctcacaaaaa
    13741 gcttcacaat aagaatgaac acggttcttc ctactcaata ccgattaaag agtttcaagc
    13801 ggaaatgact tcatttattg tttgtcacaa ttacggtatt gatacgtcag actttactat
    13861 tccttatatt gcaaattgga ctaaaaataa tgaaaaaatt gatccaaaaa actttagaca
    13921 gataatgcaa gaagttagag aaacgtctat tgattttata agtacgattg ataagaaagt
    13981 cttggaagaa cagcaaacag catttaatgc actaaaaaat tcctttgttc ctaatgagaa
    14041 taaagagcag atacaacaaa aatcagattt agaatttcat aatttctacg atagtacaat
    14101 taacgatccg gatagaacaa tccacggtag ttatttagaa gcaatggtgg attattatag
    14161 aggaaagtat ggagaaaatt ctattgagct tactcatagc aattatgatg ttacagctaa
    14221 aataggagaa aactatgtga catattattc agatacgaaa caatttgatg tatgtgatgt
    14281 ttatggtgta gtttttgaga atccaagtga aaataaaaat gatcgtttat tattagatcg
    14341 atcacctgaa aattgtgatt atgttgaaaa gtttgttgta gaaagtttga aagcttgcga
    14401 gaatcatgaa ctaaaaaatg atacgttaaa attaagcgaa ccgctaagat cagttgttat
    14461 aaatgaagcg gtttataatc tatctgaaaa tgctaacaat tactataatg aaaaatgttg
    14521 gcaagaagaa attgaaaacc aaacaaatat taatgatgta aaagaattaa actcttgtat
    14581 gactgcggaa gatttttacg aatctcataa acaagaaatc gaaaatacga ttacagattt
    14641 tgaatctgtg tcaggaacaa atttaagaga tacaatagac aatgagtcat ataaagaggt
    14701 ggcttcagtg gtagcttata aattggttgc aactaaagta aaatatgaaa tggatgaagg
    14761 aactttcaag ataccagaaa attctaactt ggaaaagccg actgtaaaaa cagcagaacg
    14821 ttcttttgaa atggagtgaa aaaattatga gtaatgaaca aaattatgaa acagacggaa
    14881 cttatcgcct gtttcatgga agtatgaata aatttgatga atttagttta gatcataaag
    14941 gtttaaacgg aacagctaat ggatttggtg tgtacttaac acctaataaa gaaatggcta
    15001 caatgtacgc tgaacaaggt agtaacggaa ataatggttt cgtatatgaa gtaaatgttg
    15061 aattagagaa gccgatttct atgaatgaac gcactattac tgatgaagag ctttctaaag
    15121 taatagataa attacaagaa agtaatgata ttttaaatga ttacaatgat gttagttatt
    15181 acggagaaaa agttgtaaaa agagaagtga ttgcttctct tagtgagaat gaaaatgacg
    15241 tagatttgat taacgacatt ggaaatacaa ttggagatca tgaagctact gctaaggcat
    15301 tttatgacgt tgggaaatat acacatatta ttgcggaaga acagttaaga caaaaggatg
    15361 tagttatcgt agtgttacag cctgataaag taaagattga aactattcac gatttaaacg
    15421 aggaacggaa tagcttagaa agtagtgagg acatgacaga taaatttgtt gggcataagg
    15481 tttcggaagt tcccattcga gaagttgtga taaaagattt tgagcaagag ctagaaacaa
    15541 ctaattatta tgacaaacaa gcttggcaag agcgaattga atacttagaa aacatggaag
    15601 aaccagtttc cagattagca ttagatagtg atattaatga attttatgat agtcataaag
    15661 aagaaattga aacgaaattg acaaatttat ccgaaaagtt agagatcgat aaagaagaac
    15721 tgctaggttt tccagtagat acttcttcta cggaatacaa aaaagctacg tctatcatgg
    15781 cttacaacgg tgcaatgaga gaaatttata atgaaattga aaatgatacg tttgaaatac
    15841 cacctgtttc tgaagaaatg gagaataaga ttttgaaatt gactaatttt gaaaatacaa
    15901 caaaatttga aatggagtag ataaatgaat gatagaacaa gaaaatagaa attttttaga
    15961 aacgcaaggt gaatatcgtc aaattaataa tagtcatttt gtaatttgtg aggatgtaca
    16021 agtcgctttt aaaatggctt tgaagtacgg aaaaagtatt agaaaaaaga taattagaga
    16081 aaaaaagagc aagaagatag gcttttggga gttatctctt agggatgggc taaactttat
    16141 tttctgtaca gaggattact atgaaaaata caagtgtacc ttatcgtaat tttactagtg
    16201 atggtctagt aaagggggtt tttgtttgga tttgaaaaag gcaaaaaaag taagtatcgt
    16261 tgatttttgc aaacaaaatg ggataggttt aaagcaagat tcaagggaat actatagatt
    16321 agaagatcat gattcttgtg taatcagtga tcgcaaaaac ctttttaaat ggaactcaaa
    16381 ggatttaggg ggagatattt ttgattttat caaagcctac tacgattgtt catttaaaga
    16441 agcaaaacag aaacttgaag atagtgatta tgaagttcat tcgttcaata caaaagtgaa
    16501 aaaagctccg taccaatatc agaaagaaaa tgaagtgccg gtatctgttg caaagtctta
    16561 tttagtaaat gagagaagaa tcgatacagc tttagtggaa gattttttat ctaaaggact
    16621 aattaaacaa gataaaaaag gaaatgtttt gttcttatgg aaaaaagatc aagaaatcgt
    16681 tgggtgtacg gaacaaggaa ctagaaaatt ttttcatgaa ggcaaacaaa aggatgttac
    16741 atggaaaaaa atacaagaaa actctaaaga gtattcagga tttaacgtaa cttttggtca
    16801 accagataaa atgtattttt ttgaaagtga aatagatttg atgtcgtaca ttacacaaaa
    16861 gccagaaaaa gccgaaaatg ctacgtttgt tagcatgaat ggattaaagg taggtaatgt
    16921 attgcatggg gttacagagc attataataa gtttaaatca ttgccttcag aaacagtcct
    16981 ttgtgttgat aatgataaag ccggacgtac ttttattgaa actgaattta aagaaaaagg
    17041 aattggaaga gaagggatag gaatcagtga agtaaaagtc gatgtacctt tgcgagaggg
    17101 atttgattgg aatgataatt taacaagagg aaaagaagct tttaaacgct tcaagttaga
    17161 agatgtatca aaaatcgtga acttcacaca taaattatct cttaatactc ctgtatatga
    17221 aatggaataa caagaagaaa gtagttgatg tgttatgaac gatacaatag caatcttatc
    17281 agattcatta attgatttag ataagacgtc tacagactat atgattattg aagaacaact
    17341 caaaaataaa aatccagagt tcgatattca agaaaaatca ttgcgtattg aattaccaaa
    17401 atttaaacaa aaaaaattaa taactattct ctttgagatt attgaacgat atgggcttac
    17461 ttattatcaa atttggacgg ataagaaaga tggccaatat acaaaagggg taagcataga
    17521 agccgttaga gaatacgatc aagtaagtat taaagcagtt caacccttaa tggggtataa
    17581 cgaagaatta ttgaattgtt taaacgtcta gtgtaaacta ggcgtttttt tacattattt
    17641 ggctacgaat agacagttaa ttaaactaaa gaaaactatt gactacaagt agtcattcct
    17701 gtatgatata gccatagact acaagtagtc aaagactcaa acccttgatt taataaaaag
    17761 gagagataat agatggagca gacaagaatg ttttggtcag atcgaatgca agatcacttg
    17821 gattgtattt acaaaaccag agagcaatta aataagttag atactgtcag atattatttt
    17881 acctacttca gtgagaatga cgaacaaaga tgggatattt ttcatgatgg taattacgaa
    17941 gaattttttt acactgaaag ttaattattt gttgaaatga tgaaggattc tatttgatga
    18001 aagggaacgg aaaaccgttc ccctaaaaaa tattcaaatg actacaagta gtcatagaaa
    18061 gagagcggaa taatggtaaa taaaaaagaa acgtttaaag tagggatgga agtacagggc
    18121 gttgttattg ggagcggaga gcttatcgag ggagaagtaa ttagtttgtt agctaatgta
    18181 gcggtagtta aagcaatcgg aaaagcaccg gtagtgatga aaaaatgcag catgatgtgt
    18241 cttgatggtt taaacaatcc aataactcaa cgctttgaga acgtgatata atagaaaaaa
    18301 gaagattttc tactgggaga gattattatg aaagactatg ctttacgtaa atttatccac
    18361 ttagagccta tgaaagaatt ggttattaat gggaatgaat ataatgatta caaatcttta
    18421 tttgtaaagg aacagcctaa aggtggtatt cggatgaaaa atttaccgga cttccgactt
    18481 attgaagtaa acaatttagg aattatagaa gactatttag ctaagttaga tgaagcggta
    18541 aaacaatcaa ttgaaaaaaa tggtgaagac catgtactta cggttgtctt aaaagacttg
    18601 aaagatagac tagaagctga agcagagaac tttaataaag actaataaga agggaatgag
    18661 agatatgacc gaggctgtaa aagaattaaa gaaaatgtat cctgatgttt tgaatatgac
    18721 tgtagatgat tttcacgaag ccttaaaaaa tgcagaaagt gaggaagaaa ggactttcta
    18781 ccttacactt tctagttttg tgacacgagt agatcaaaaa aaagtgatta atcagaaaga
    18841 ttttaaaata tgagtttagg aaatgagaaa ccatattatg tagttattgg gggagtaaac
    18901 ggcgccggta agtctactat ttatcaaatt caaaatttaa aaagaaaaga acttgatact
    18961 gatagaatta atgcagatga aattttgaga agtttcggcg gaaattggaa aaaacaagca
    19021 gatataaaca aaagtatgcg tgaagttata agagagatca aagatcattt taaagaagga
    19081 aaatcttttc accacgaaac agcgtttgtt ggtaatgaat atccccataa aaagagactt
    19141 ctaaatgcaa aaaaattagg ctatgaatca tttttaatct atgtagggtt agattcacca
    19201 gaaaaggcga ttgaaagggt taaaagtcgt gttgctaaag gtggtcatgg ggtaggcgaa
    19261 gatttaataa gggaacgata cgtatcttct ctaagaaact tagcaggatt agaaaaatat
    19321 tttgataatg taagtatcta tgataattca gatgagtttt cttatattta tgttaaagaa
    19381 aatcatacag ttgtagccaa taatacgcaa gaatataatt gggttcccaa agctattcaa
    19441 gaaaaaagag ctgaaattcc tataagtaaa caaattatca aggatatatc ccaagaaggg
    19501 ttgagtccaa aagaaaccct tgaaagaata tattcagaaa gtgatgattt tattagtgat
    19561 cgttttttta aagcagagca agttgatgat ttttatatga aacataaaaa tgaaatcaag
    19621 gaacgggttc tagcaattag tttttctaca ggagtatctg aagatgaatt agtaggccaa
    19681 gataaaaaaa gaaattcttg taagttggca tatattaata gtgtaggcga gcttttagaa
    19741 aaaactgata gtgagctatc gagcatggaa gcaagcagag atagaagtat aaaaaaaact
    19801 gattttgaaa ttgaataaaa gaagatggag ctgaatcagt taatagtaaa taaatttaat
    19861 aaggagtgcg aaagtacttc ttatttttta aataaaatga ctacatgtag acgaaaggga
    19921 taatatatta tttttagttc ttgtttttaa aattactatt tgctataatg aacttaaata
    19981 aatttttctc agaattttat ttgctataat agactcaaat aaagttttct tagaacttta
    20041 ttcattatcg agtgttcccc tttgtctgca aaactatcgg gagcactctt tttcattttt
    20101 tcaataagtt ctgtaaccat atagaacagc acgcttctga aatgtgtaga taaacagctc
    20161 ttaaatattt atgagtcagt atatcattta agtcttagtt acatgctaag actatttttt
    20221 tgttttgtgt gttacgtaag ggtataccct attgtatcat atggtaaagt acagtataat
    20281 aaggtttgga ggtgtaacat tagggtgtaa cacaatgtga aatttggagg tcattttttg
    20341 aacaagaaac aggatcaaga atattatata gaaaaattgg agaaagagaa cttagaactc
    20401 aaagagcgaa taagatacta tgaaaccaaa ttccataaga gaagtgattg tatgaagccg
    20461 aatccttcag aagcaggaaa acgaattaaa caattacgcc tttcatgtgg ttttactatg
    20521 gaagaacttg gaaggaaaat agataattca ccacgtgcga ccatatccaa ctgggaacgt
    20581 gggaccaatt tgccaaatcc acaaaagctg aaattattgt caactatcac taattcaaca
    20641 attgactgga ttaaatgggg aactttggaa gagtacataa cttcatacct tatcggtata
    20701 ggatatgagc tatatattaa agactttcca gagatacctc acaaagtctt taaagacatt
    20761 caagaaaaat atagtgatac gttttcgtta gataaagact atgaattatt ggattctata
    20821 ataaaaaata tatttacaaa atattactca aaagaatttg aagtaaacca aattccataa
    20881 gagaggtgat tgtatgaaac caaatcctgt tgaaacaggt aaaagaatta aatcaattcg
    20941 ttctaatttt ggaatgacta tggaacaatt tgcagattta attggaaatg ctcaaaaagg
    21001 aaatattaac aattgggaac gaggattctc ccttcccaac aaagaaagat taattaaaat
    21061 tgctgaatta agtaacaaaa ctatcgactg gattaaatgg ggaactttgg aagaatacat
    21121 agcttcatac cttaaagatt cagggtatga actatatatt aaagacttcc cagagatacc
    21181 tcacaaagtc tttaatgaca ttcaagaaag atatagtgat acgttttcgc taaataaaga
    21241 ctatgagtta ttgaatccta taataaaaaa tatatttaca aaatattact caaaagattt
    21301 tgaagattat cgagacatag aagttaaacc agaccctaaa aaggtaggga gaaaaattcg
    21361 ttctattaga aaaaaattag gccttactat gcaggaattt gggtgtgagg tttctaattc
    21421 tcctagaagc actgtgtcaa catgggaaca tggtggtaac ttacccaaca aagccaaact
    21481 aagaaaaatt gcagacattg cgaattgtag tgtagaagat ttgctttttg atgaaggctt
    21541 tatttttaaa caatccaaag ttaaattgat aaatgaactc aaagaagaaa attcaaagct
    21601 aaaagaaaaa ataaaatact atgaatgttt atttgatggt atagagagtc tgctgaactc
    21661 acgaaaaaat tcctagttaa ttaaactaga gtaaaattat ttgtaggtgt taaatatgtt
    21721 agtgggttat gcacgggtca gttcagatga tcaaaactta gaaagacagc ttgaagaatt
    21781 tagaaaaatt ggtgttgaaa aagtatttgc tgagaaaaaa tctggccgta acacagtaga
    21841 acggccagag ttcaataaaa tgttgtcatt tgtcagagaa ggggatacgc ttgtttttga
    21901 gtcgcttgaa cgcttaggaa gaaattctga tgaaatacta gaaacagtat cttttataga
    21961 taaaaatggt atgggcttaa tggttttgaa tttaccaatt ttagatacaa aatttggtga
    22021 tccaaactta gaaaaacttg ttagaagctt agtaattaac attttttctt ggacggctca
    22081 aaatgagagg gaggaaatta agagaaaaca aaaacaagga atagcaattg cgaagaagaa
    22141 aggagtctat aaaggaaaac cctacgaata ttcttctact gcaaagaatc cacaaaagag
    22201 gtttatctat aatgaaatag taagaatgct tggagaaggt gttccgatta agcgaattgc
    22261 agaaaaaaca ggaacaaata gaaatttagt ctatagaata aagaaaagaa taagtgaatc
    22321 tgattgattt taagttcttt gagaaatcaa tgttacgaga gaaggattct aagcctgttt
    22381 caaactttta aggtgtacaa ttatacaaag aagcttctaa acaaaagaat taattttgtg
    22441 gcagtcaagt aacgacagaa atagaaaaga aagaggctat gcgagagtga atcaacaagt
    22501 taattgggtt aatgattttg aaaaagaatt tcgactgaaa ttttttggaa atcctacagc
    22561 agaaagtaca gaagaaaaaa atgtagtcta ttttgaaaac acaagaaaca cggaacgggc
    22621 tgtcaaaata gaccaaaata tcaaagttag aatatgacga aaacagattc aatacatttc
    22681 cttgcaatac cattcaagaa tcaattgata agcatgagtg ataattgatt atctacagga
    22741 aggtgttttt tatgaaatta gagatcaaag aagttgtctg cgattgggga atttatgtag
    22801 acggagaaac atatccattt atgattttta atagtaaagc aaatgcacaa gagataatgc
    22861 gtatcatgga gttggataat aagcacgaaa gatttgatta agtaaacaaa atggagaatg
    22921 aatgtgaagc agtggaatac caaaaaacat gcgccaataa taagtatatt ttaggtgcaa
    22981 tgggattagg taaatcagct tctattaaac aggtctacga agaatcacag gagaattgcg
    23041 tatatgaaaa ttcgaattaa agagtgagaa tatgtgggag ctaatttatt tagttttata
    23101 cttaatagga aaatcaactg aatttgtata taacaataag gtaatattta tattgttctt
    23161 aatagtttgt attatcgtat ttgatgtcta tgatttacta attcataaga atgaaaatag
    23221 taatattggt agaattataa tcacaaactt aatagtaatt actattggga ttacaacatt
    23281 aacaaataaa gctttaatat caagtatgat tttaatagtg tttgatttta ttatcattta
    23341 tagtttgctg tttttaataa agaaagtaac tgaatattta aatcgttagg cacgaaataa
    23401 attttaattg aggttgaatt atagcggtaa atcatacaaa atagaaatgt agtcttgtta
    23461 agcgtcttga ctatctaatt ttgaatgagg agggttgtat atagaaaaag ggtagttaaa
    23521 ttcgtagatc aaaaggctac gaaggagaat aaagaatgac agctatttat aaagcactaa
    23581 aaaattctaa cgtatcagag tacgttaagt cgagagtatt aaataataat ttagattaca
    23641 ttttagaaaa tggtgttgct gatttaatca gtaaattaag tgatcctata gatatagatc
    23701 atttggtggt atggcaagat ggtactttag aagtatcaat atatggagaa ttttatagta
    23761 gaaataaagt aactacattc aaaaatagta ttagtggtcg agaagattta aaaggaatca
    23821 ataaaaatag taaattctat agaacaaata aagaattgct agaaaaaatt tcaaagcgtg
    23881 tttttgaatt ggtgaatcca ttttcaaaaa aacgtagtaa agaacttttt ggaatttttt
    23941 atgatggttt tagtgcaaaa agtaatgtag aaaaattttt ttattttaat gattcagctt
    24001 atattgcggt taaagacagt aaaagaatcc ctgaagcaac tttagaatat ttagagccaa
    24061 ttaaattgtc tgttttttat tcactttatg agaaagaaat cgctgatgaa gcggtttaag
    24121 gggagtgaaa tgagtaaaaa aataggcaaa gagtcattat cgctcggatt gaaaaaagaa
    24181 attgttaaat ttaaaagtat gtatagagag ccggaattta gactcatttt tcaattagat
    24241 atagtgagag ggctgaaaat tagttttagt tgtttaggtt atctggtctg gctattcatt
    24301 gctagagtag gatggagcaa tgcttatagc ttttataaac tgttcgccgg aactgatcga
    24361 ttaaaagaat cattagaggt gaaagaagcc ttaaatattt taggaagtct tgctttcagc
    24421 ctgtttattt ttgctttatt tatcttacaa gtaggaaaaa taatgatgtc aacaaatgac
    24481 aattatatta attatttgaa gccttttcaa aaaggaataa aaatcatttg tagacagggc
    24541 acagttttat ttctacttag cctgtatgag catgaaattc attttcaaaa ttattataat
    24601 acgacaacta aacttggtgc atttttactc ctatatttag ttagtaatat agctgaaaaa
    24661 atgctgttta aacatattgt aaagcaagtc ttttctgata ttcaaaagga aagctattgt
    24721 ttaaaaacac cttatggaaa tttaaaaaat gttgagctg
//
LOCUS       DANKHJ010000035        24261 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000035 DANKHJ010000000
VERSION     DANKHJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="PW113_001583"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="PW113_001583"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2525900.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="PW113_001584"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="PW113_001584"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2525901.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="PW113_001585"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="PW113_001585"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2525902.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="PW113_001586"
     CDS             complement(3373..4680)
                     /locus_tag="PW113_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2525903.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="PW113_001587"
     CDS             complement(4683..5264)
                     /locus_tag="PW113_001587"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2525904.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="PW113_001588"
     CDS             complement(5836..7128)
                     /locus_tag="PW113_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2525905.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="PW113_001589"
     CDS             complement(7125..7748)
                     /locus_tag="PW113_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525906.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="PW113_001590"
     CDS             complement(7751..8587)
                     /locus_tag="PW113_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525907.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="PW113_001591"
     CDS             complement(8604..9491)
                     /locus_tag="PW113_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525908.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="PW113_001592"
     CDS             complement(9507..10790)
                     /locus_tag="PW113_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2525909.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="PW113_001593"
     CDS             complement(11473..12156)
                     /locus_tag="PW113_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2525910.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="PW113_001594"
     CDS             complement(12174..12791)
                     /locus_tag="PW113_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2525911.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="PW113_001595"
     CDS             complement(12801..13625)
                     /locus_tag="PW113_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2525912.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="PW113_001596"
     CDS             complement(13618..14178)
                     /locus_tag="PW113_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525913.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="PW113_001597"
     CDS             14352..15308
                     /locus_tag="PW113_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2525914.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="PW113_001598"
     CDS             complement(15613..16569)
                     /locus_tag="PW113_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2525915.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="PW113_001599"
     CDS             16673..17275
                     /locus_tag="PW113_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2525916.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="PW113_001600"
     CDS             complement(17363..18862)
                     /locus_tag="PW113_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2525917.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="PW113_001601"
     CDS             complement(18878..19402)
                     /locus_tag="PW113_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2525918.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="PW113_001602"
     CDS             19700..20449
                     /locus_tag="PW113_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2525919.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="PW113_001603"
     CDS             20440..21225
                     /locus_tag="PW113_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2525920.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="PW113_001604"
     CDS             21194..21910
                     /locus_tag="PW113_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2525921.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23046)
                     /locus_tag="PW113_001605"
     CDS             complement(22012..23046)
                     /locus_tag="PW113_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2525922.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23050..24108)
                     /locus_tag="PW113_001606"
     CDS             complement(23050..24108)
                     /locus_tag="PW113_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2525923.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5221 c         4225 g         7626 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt a
//
LOCUS       DANKHJ010000036        23831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000036 DANKHJ010000000
VERSION     DANKHJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(55..789)
                     /locus_tag="PW113_001607"
     CDS             complement(55..789)
                     /locus_tag="PW113_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2525924.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="PW113_001608"
     CDS             1116..1526
                     /locus_tag="PW113_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2525925.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="PW113_001609"
     CDS             1519..2220
                     /locus_tag="PW113_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2525926.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="PW113_001610"
     CDS             2220..3806
                     /locus_tag="PW113_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525927.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="PW113_001611"
     CDS             3825..4046
                     /locus_tag="PW113_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2525928.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="PW113_001612"
     CDS             4043..4804
                     /locus_tag="PW113_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2525929.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="PW113_001613"
     CDS             5184..5693
                     /locus_tag="PW113_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2525930.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="PW113_001614"
     CDS             complement(5768..6307)
                     /locus_tag="PW113_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2525931.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="PW113_001615"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="PW113_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2525932.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="PW113_001616"
     CDS             7320..8171
                     /locus_tag="PW113_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2525933.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="PW113_001617"
     CDS             8199..8834
                     /locus_tag="PW113_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2525934.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="PW113_001618"
     CDS             8854..9627
                     /locus_tag="PW113_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2525935.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="PW113_001619"
     CDS             10035..10832
                     /locus_tag="PW113_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2525936.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="PW113_001620"
     CDS             10810..11223
                     /locus_tag="PW113_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2525937.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="PW113_001621"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="PW113_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2525938.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="PW113_001622"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="PW113_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2525939.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="PW113_001623"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="PW113_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2525940.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="PW113_001624"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="PW113_001624"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2525941.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="PW113_001625"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="PW113_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2525942.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="PW113_001626"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="PW113_001626"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2525943.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="PW113_001627"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="PW113_001627"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2525944.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="PW113_001628"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="PW113_001628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2525945.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="PW113_001629"
     CDS             21907..22485
                     /locus_tag="PW113_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2525946.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="PW113_001630"
     CDS             22537..23019
                     /locus_tag="PW113_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2525947.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="PW113_001631"
     CDS             23136..23510
                     /locus_tag="PW113_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525948.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 cagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatggggt attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DANKHJ010000037        22634 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000037 DANKHJ010000000
VERSION     DANKHJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(228..1115)
                     /locus_tag="PW113_001632"
     CDS             complement(228..1115)
                     /locus_tag="PW113_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2525949.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="PW113_001633"
     CDS             complement(1112..1981)
                     /locus_tag="PW113_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2525950.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="PW113_001634"
     CDS             complement(2118..3404)
                     /locus_tag="PW113_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2525951.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="PW113_001635"
     CDS             3677..4255
                     /locus_tag="PW113_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2525952.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="PW113_001636"
     CDS             4255..5184
                     /locus_tag="PW113_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2525953.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="PW113_001637"
     CDS             5181..6917
                     /locus_tag="PW113_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2525954.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="PW113_001638"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="PW113_001638"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2525955.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="PW113_001639"
     CDS             complement(7813..8322)
                     /locus_tag="PW113_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2525956.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="PW113_001640"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="PW113_001640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2525957.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="PW113_001641"
     CDS             complement(9623..10714)
                     /locus_tag="PW113_001641"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2525958.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="PW113_001642"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="PW113_001642"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2525959.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="PW113_001643"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="PW113_001643"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2525960.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="PW113_001644"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="PW113_001644"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2525961.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="PW113_001645"
     CDS             complement(14677..15651)
                     /locus_tag="PW113_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2525962.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="PW113_001646"
     CDS             complement(15632..17515)
                     /locus_tag="PW113_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2525963.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="PW113_001647"
     CDS             complement(17533..19758)
                     /locus_tag="PW113_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2525964.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="PW113_001648"
     CDS             19873..20907
                     /locus_tag="PW113_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2525965.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="PW113_001649"
     CDS             complement(20974..21987)
                     /locus_tag="PW113_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2525966.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="PW113_001650"
     CDS             complement(22065..22253)
                     /locus_tag="PW113_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2525967.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6446 a         4863 c         4096 g         7229 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taac
//
LOCUS       DANKHJ010000038        22465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000038 DANKHJ010000000
VERSION     DANKHJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            144..542
                     /locus_tag="PW113_001651"
     CDS             144..542
                     /locus_tag="PW113_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2525968.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="PW113_001652"
                     /pseudo
     CDS             526..1220
                     /locus_tag="PW113_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="PW113_001653"
     CDS             complement(1814..2701)
                     /locus_tag="PW113_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2525969.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="PW113_001654"
     CDS             2886..3725
                     /locus_tag="PW113_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2525970.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="PW113_001655"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="PW113_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2525971.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="PW113_001656"
     CDS             5193..5549
                     /locus_tag="PW113_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525972.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5552..6709
                     /locus_tag="PW113_001657"
     CDS             5552..6709
                     /locus_tag="PW113_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2525973.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="PW113_001658"
     CDS             complement(6735..7175)
                     /locus_tag="PW113_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2525974.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="PW113_001659"
     CDS             7335..7841
                     /locus_tag="PW113_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2525975.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="PW113_001660"
     CDS             8009..9922
                     /locus_tag="PW113_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2525976.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="PW113_001661"
     CDS             9927..10928
                     /locus_tag="PW113_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2525977.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="PW113_001662"
     CDS             complement(11130..11336)
                     /locus_tag="PW113_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2525978.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="PW113_001663"
     CDS             complement(11982..12830)
                     /locus_tag="PW113_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2525979.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12829..13896
                     /gene="gnd"
                     /locus_tag="PW113_001664"
     CDS             12829..13896
                     /gene="gnd"
                     /locus_tag="PW113_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2525980.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="PW113_001665"
     CDS             13898..14899
                     /locus_tag="PW113_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2525981.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="PW113_001666"
     CDS             14896..15180
                     /locus_tag="PW113_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2525982.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="PW113_001667"
     CDS             15213..16010
                     /locus_tag="PW113_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2525983.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="PW113_001668"
     CDS             16024..16857
                     /locus_tag="PW113_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2525984.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="PW113_001669"
     CDS             16875..17732
                     /locus_tag="PW113_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2525985.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="PW113_001670"
     CDS             17754..18263
                     /locus_tag="PW113_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2525986.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="PW113_001671"
     CDS             18300..18701
                     /locus_tag="PW113_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525987.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="PW113_001672"
     CDS             18813..19166
                     /locus_tag="PW113_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525988.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="PW113_001673"
     CDS             19200..19643
                     /locus_tag="PW113_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2525989.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="PW113_001674"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="PW113_001674"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2525990.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="PW113_001675"
     CDS             20738..21451
                     /locus_tag="PW113_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2525991.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="PW113_001676"
     CDS             complement(21658..21807)
                     /locus_tag="PW113_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525992.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="PW113_001677"
     CDS             22203..22403
                     /locus_tag="PW113_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2525993.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3452 c         4641 g         6626 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aattc
//
LOCUS       DANKHJ010000039        22423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000039 DANKHJ010000000
VERSION     DANKHJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            6..305
                     /locus_tag="PW113_001678"
     CDS             6..305
                     /locus_tag="PW113_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2525994.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PW113_001679"
     CDS             378..1739
                     /locus_tag="PW113_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2525995.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PW113_001680"
     CDS             1761..2021
                     /locus_tag="PW113_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2525996.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PW113_001681"
     CDS             2035..2787
                     /locus_tag="PW113_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2525997.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PW113_001682"
     CDS             complement(2881..3486)
                     /locus_tag="PW113_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2525998.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PW113_001683"
     CDS             complement(3539..4513)
                     /locus_tag="PW113_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2525999.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PW113_001684"
     CDS             complement(4891..5610)
                     /locus_tag="PW113_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526000.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW113_001685"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PW113_001685"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2526001.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PW113_001686"
     CDS             7161..8558
                     /locus_tag="PW113_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2526002.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PW113_001687"
     CDS             8639..9079
                     /locus_tag="PW113_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2526003.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PW113_001688"
     CDS             9227..9553
                     /locus_tag="PW113_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2526004.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PW113_001689"
     CDS             9554..10303
                     /locus_tag="PW113_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526005.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PW113_001690"
     CDS             complement(10383..10838)
                     /locus_tag="PW113_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2526006.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PW113_001691"
     CDS             complement(11265..11540)
                     /locus_tag="PW113_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526007.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PW113_001692"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PW113_001692"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2526008.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PW113_001693"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PW113_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2526009.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PW113_001694"
     CDS             14770..17436
                     /locus_tag="PW113_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2526010.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PW113_001695"
     CDS             17800..18552
                     /locus_tag="PW113_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2526011.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="PW113_001696"
     CDS             complement(18574..19728)
                     /locus_tag="PW113_001696"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2526012.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="PW113_001697"
     CDS             19913..22354
                     /locus_tag="PW113_001697"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2526013.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7199 a         3933 c         4672 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DANKHJ010000040        21501 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000040 DANKHJ010000000
VERSION     DANKHJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..296)
                     /locus_tag="PW113_001698"
     CDS             complement(<1..296)
                     /locus_tag="PW113_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HDL2526014.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEE"
     gene            complement(293..829)
                     /gene="cysE"
                     /locus_tag="PW113_001699"
     CDS             complement(293..829)
                     /gene="cysE"
                     /locus_tag="PW113_001699"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2526015.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(915..1154)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1151..2611)
                     /locus_tag="PW113_001700"
     CDS             complement(1151..2611)
                     /locus_tag="PW113_001700"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2526016.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(2781..3938)
                     /locus_tag="PW113_001701"
     CDS             complement(2781..3938)
                     /locus_tag="PW113_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2526017.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(3972..5342)
                     /gene="radA"
                     /locus_tag="PW113_001702"
     CDS             complement(3972..5342)
                     /gene="radA"
                     /locus_tag="PW113_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2526018.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(5405..5932)
                     /locus_tag="PW113_001703"
     CDS             complement(5405..5932)
                     /locus_tag="PW113_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2526019.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            6162..6968
                     /gene="proB"
                     /locus_tag="PW113_001704"
     CDS             6162..6968
                     /gene="proB"
                     /locus_tag="PW113_001704"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2526020.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            6965..8212
                     /locus_tag="PW113_001705"
     CDS             6965..8212
                     /locus_tag="PW113_001705"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2526021.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            8288..8632
                     /locus_tag="PW113_001706"
     CDS             8288..8632
                     /locus_tag="PW113_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526022.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(8749..9075)
                     /locus_tag="PW113_001707"
     CDS             complement(8749..9075)
                     /locus_tag="PW113_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526023.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(9690..10892)
                     /locus_tag="PW113_001708"
     CDS             complement(9690..10892)
                     /locus_tag="PW113_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2526024.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            11009..11524
                     /locus_tag="PW113_001709"
     CDS             11009..11524
                     /locus_tag="PW113_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2526025.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(11723..12169)
                     /locus_tag="PW113_001710"
     CDS             complement(11723..12169)
                     /locus_tag="PW113_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2526026.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(12216..13694)
                     /gene="msr(C)"
                     /locus_tag="PW113_001711"
     CDS             complement(12216..13694)
                     /gene="msr(C)"
                     /locus_tag="PW113_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2526027.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(14048..14677)
                     /locus_tag="PW113_001712"
     CDS             complement(14048..14677)
                     /locus_tag="PW113_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2526028.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(14661..15125)
                     /locus_tag="PW113_001713"
     CDS             complement(14661..15125)
                     /locus_tag="PW113_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2526029.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(15612..16880)
                     /locus_tag="PW113_001714"
     CDS             complement(15612..16880)
                     /locus_tag="PW113_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2526030.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(17111..17848)
                     /locus_tag="PW113_001715"
     CDS             complement(17111..17848)
                     /locus_tag="PW113_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2526031.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(17915..18484)
                     /locus_tag="PW113_001716"
     CDS             complement(17915..18484)
                     /locus_tag="PW113_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2526032.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            18612..19484
                     /locus_tag="PW113_001717"
     CDS             18612..19484
                     /locus_tag="PW113_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2526033.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            19826..20698
                     /locus_tag="PW113_001718"
     CDS             19826..20698
                     /locus_tag="PW113_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2526034.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            20695..>21501
                     /locus_tag="PW113_001719"
     CDS             20695..>21501
                     /locus_tag="PW113_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2526035.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENK"
BASE COUNT         6194 a         4497 c         3984 g         6826 t
ORIGIN      
        1 tcttcttcaa atgctcggat gaaacgatca gcaacttctg gtgctgtaat acctaattcg
       61 tttgctgtac ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa atcgacttta
      121 tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag caatggtgct acgagcattt
      181 ccgatatgga tataattata cacagtcggt ccgcagacat acataccgac atgtcccttt
      241 tgtataggga tgaattcttc tttttttctg gtcaatgtat tgtagatttt tatcattctg
      301 atctcctcac tttttctcct ttgattcgaa cgacttttgc tggtactcct accgctgtag
      361 cgtcgtctgg aacatctttc aacacaacgg cacctgcacc gattttggcg ttttttccga
      421 tcgttaccgg tcctaatatc tgcgcattcg cagagagcat ggctccttgt ttaacagttg
      481 ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt tacgccatgg aataaaatca
      541 catcgtcctc gatttctgcg gtttcgccaa tgacgattcc cattccatgg tcgatgaaca
      601 cgccagttcc aatcgttgct cctggatgga tctcaacacc agttaggaaa cgccagaatt
      661 gagcattgat cttcgccaat aaaaagagcc ggtgctgata taaaaaatga gagaaacgat
      721 gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag
      781 ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg actttccact
      841 cctttcgtaa gaattttatg taggaaatca tcttcttata aagaaaagaa gcgcctttta
      901 aataagtttc cttatcaaaa gacgctttcc gcgtggttcc acttttcttc ataatcaatg
      961 attatctctg acagctaacg cctgttatac gttcttggct actttgtttc ctataaaaca
     1021 gtttttcctg ttttctgtgt ttcgccaaga ccactcccag aggcatttcg gcaaccattc
     1081 attgatacct tgcaccaccc ggtatctctc ttgtaatgat cagctgctta cttttttctg
     1141 ttcatcgtgt ttagttcagt acttgattta aatgtgctaa tgatttttca cgaccaagca
     1201 attcgatcgt ttcaggcaat tctggaccat gcatttgtcc tgatacagct acacggattg
     1261 gcatgaaaag attttttcct tttacgccag tttctttttg aacagctttg atggcagctt
     1321 tgacagttgg agcatctact acttccattt gttctagctg tgctttgaat gcgtggagaa
     1381 cagtaggaac tgtttcacca gcaagtactt cttttgctgc ttcattcaat acaggatgtt
     1441 cattgaaaaa tagcgtagac agatcaacga tttctgcagc atagctcatt tgaggttgat
     1501 acaggctaac gatcttcttc acccattcga ttgtcttttc atctgggttt tctgctacac
     1561 gtccatcagc gattagataa ggcagacaca tgtcagtaaa tgcatctaga tccattgctt
     1621 tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac
     1681 ttaggcgttt tggatcgaac attttgatca attcttggtg ttcgaagatt tcgtcttcgc
     1741 ccacaggtga ccagcccaat aatgcaataa agttgaacat tgcatctggt agatagccta
     1801 attcgcgata ttgttcgata aattgcaaga ttgattcgtc acgtttgctt agttttttac
     1861 ctgtttcact gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg
     1921 cttcatagat catcagttgt tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt
     1981 gagtgatttt catcaaatga tcatcgaccg caacagcaaa attataagtt ggcataccat
     2041 cgcgtttttg gatgacaaag tcgccaccga tattatcgga ttcgaacgaa atagcgcctt
     2101 tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt acaacagatt
     2161 ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt caagttcgca catgttcctg
     2221 cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt
     2281 ctgtgcaata gcatttgtat gctcgattgc tagcaagcag ctgatcaatc agcggttgat
     2341 agatttcttt acgttcagat tgacggtaag ggccgtattc tccaggattt tcaggagatt
     2401 catcccaatt cattcccaac caagcaaggt tttccaactg gcttttttcg ccatcttcga
     2461 tattacgttt ttggtcagta tcctcaatac gaataataaa gtctccatca ttgtggcggg
     2521 caaacaaata attgaataat gcagttcttg catttccaat atgcaggtgc ccagttggac
     2581 ttggtgcata gcgtacgcgt actttcgtca ttttcttcct cttctttcaa actagacttt
     2641 gactaacagc aaaaattccg tcagttacgg cttctcaaat atcatttatt taaagccact
     2701 cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc aactggttga atttccttac
     2761 cagttggctg aatttctttt ttattttgat tgtttagtct ttttctctgt ctctttttta
     2821 ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt
     2881 tgaagggcac ttgttacaac gacttcgata cgttcattca tgtaatgctg tccatcttct
     2941 acgacaacca tcgtgccgtc atcaaggtag gctactcctt gttggcgttc tgtcccggct
     3001 tttactacta gaacattcat cgtttcacca gggatgacga ctggttttac ggcatttgcc
     3061 aacgcattga tattaagaac aggtacattt tggaattcag agactttatt caagttatag
     3121 tcattggtta caatgacacc atctaaaagc ttagccaatt tgatcaattt gctgtctact
     3181 tcactgatat cttcaaagtc tccatcgtac atttcgacag atataccctc ttctttttgc
     3241 aaggcattta ggatatctaa tccgcgacga ccacgcacac gtttcaaact atcccctgag
     3301 tccgcgatgt attgtagttc gtaaagaaca aaattaggga tcatcaaggt tccttctaag
     3361 aatccagtct ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt atccaagatt
     3421 ttatatttat ggaaattttc tcctgatttt cgttctaaca gttgactctc aacttgacta
     3481 gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt
     3541 cgtgtcgttc ccattctcaa tcccagatag cctaagatga tcatcagtaa aatcggcaat
     3601 acactattga cgaatggaat ttccatattg taaagagggg tcgagatcac aacacctaaa
     3661 gtcaatccga tgatcgcacc gatcgcaccg aagagaagat aagtcaagct gacctcactt
     3721 agtttttgtt ctacttgttt gataatagct gagatatgtt tggccaacag caaagataat
     3781 aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg tattattcag ccatccgttt
     3841 gactgttgac cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg
     3901 acaatgatca tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa
     3961 tcatttatta gttagcggaa cacacgcttc aacgtttcag ccaaagttgc tactccaact
     4021 atctcgattc cttttggcgc ttcccagtta cctaagttgt ttttaggtag gtatactttt
     4081 gtgaagccga gtttttggac ttctttgact cgttgttcaa tatgactgac acgtctgatt
     4141 tctccagtca gtccaatttc accgatgaaa cattccgttg gctgtgttcc tttttcttta
     4201 tagcttgaag caatactgac agcgatagct aaatcaatcg ctggttcatt taattttacc
     4261 ccacctgcag cttttaagta agcatcttga ttctgaagaa gcaacccagc tcttttttcc
     4321 agcactgcca tgatcagaga tacccgattg aaatcaagac ctgttgttgt tcgtttggca
     4381 tttccgaaca tagtaggtgt gactaatgct tggatctcta ccaaaattgg acgtgtccct
     4441 tccattgcca caacgatagc agagccagtt gcaccgtcta ggcgttcttc taagaaaatc
     4501 tgtgaaggat tagcgacttc ttccaatcca tgctcatgca tttcaaaaat cccaatctca
     4561 ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc gaaaactatg atgttgttca
     4621 ccttcaaaat ataacaccgt atccaccata tgttctaaca ttcttgggcc ggcgatcgag
     4681 ccttctttgg taacatgacc gacgataaaa atggctatcc catttgtttt agcggttttg
     4741 agtagttctg cagtggtttc tcttacttgg ctgacacttc ctgccacact tgtgatgtct
     4801 ggctgcgtca tcgtttggat ggaatcaatg ataacataat caggggagat atgttcgatg
     4861 gcacgactga tttcgttcat atctgtttca gcgtatagat aaaattctgt gttgatactt
     4921 ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga tacatataag
     4981 acttttccgc ctatggctgc taattgctga gaaacttgta acagcaaagt ggatttcccg
     5041 atacctggat cgccaccaat caagaccata gaccctggca ccacacctcc gcctaacaca
     5101 cggttcagtt cttctaattt tgttttgatc cgaggttctt ttttagggac gacgtctgcg
     5161 atttttgttg gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta
     5221 tcttgttcga tttcttcgac catcgtattc cattttccgc aatttgggca gcgtcccaag
     5281 aacttaggcg acacataccc gcaattttgg caaacaaatt gtacttttga tttttttgcc
     5341 attgtaccgc ctccttttat cctaatcatg agacattaaa tcaagcttaa gtatctttaa
     5401 cgttttattt actggaagaa ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat
     5461 ctgcttttaa aaacggcaaa aagatccctt ggccgatacg ctcgcctttt ttgatcacta
     5521 catccttgaa accaaagttg gtgaattgga acatgatatg gccttcattt gcctcattat
     5581 tgtaataatc actgtcaatc acacctactc cgtttgtcaa cactaaaaac cgcttcaacg
     5641 gattgcttga acggttagca agctgcaaaa attcatcttc tcccatgtaa gctttgattc
     5701 cagtaggcac gagggttggt ttcaattgtt tttcattttc tgatacatct tttttcaatc
     5761 catctgcgac tgtttccaac ccctttttcc agcctgcttt ccagatactt ggaatcacga
     5821 cctcttctgc tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc tgcggtagat
     5881 tgatattttg atctttatat ttagaaataa cttcaaatcc tctttttctc accggttttc
     5941 ctccctaaat aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca
     6001 aattcttcaa gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg
     6061 actgcaagtt gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa
     6121 tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa
     6181 atgcaaaacg aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata
     6241 tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa
     6301 aggaagtgat cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt
     6361 cagaacgtcc gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac
     6421 tgatgaatat ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg
     6481 tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac
     6541 agcttttgat tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg
     6601 aattagatca tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac
     6661 ttgtcgcggc agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc
     6721 cgactacaaa tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc
     6781 tggcacaagc aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa
     6841 aagccgcttc taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc
     6901 cggcaattat ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact
     6961 tgaaatgact gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc
     7021 acttatgcct acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa
     7081 gaatcaacaa gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa
     7141 tatcagtgaa acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc
     7201 tacagagatc gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg
     7261 gacgaacacc gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat
     7321 catttacgaa tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg
     7381 gaatgccgtt atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca
     7441 attattgcag caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga
     7501 tgatacttct catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct
     7561 gattcctcga ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat
     7621 cattgaaaca ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat
     7681 ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga
     7741 aacattgctg atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact
     7801 ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc
     7861 tgctttagca gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa
     7921 agtggtcgat tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc
     7981 agaaacgatc gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc
     8041 ggcggctgtc tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg
     8101 tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga
     8161 attaacttcc acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt
     8221 gtgaaacatt attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg
     8281 tgtcaatatg tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc
     8341 tgcttttctg ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg
     8401 gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac
     8461 acgcttgcac attagttaca tggttgcatt atgcttatta gcgacaatca attactattc
     8521 ttttttcgat cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt
     8581 atcttataaa tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc
     8641 cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg
     8701 tgacaaagat tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc
     8761 tggtaaatct gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata
     8821 ataacgaagc agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga
     8881 ttcagccagt agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc
     8941 ttttctagtt tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat
     9001 caaccgatca tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa
     9061 atattcttcc agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct
     9121 actgaaaatt ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt
     9181 cacatcccct ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca
     9241 atattcaaca aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac
     9301 gacagtctca caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg tattagctca
     9361 aaaaacggta aaatatgaga agctattttt ccgttttgtt tctctaatac ttaggataaa
     9421 acgctgattt tacaacctct gttaaacggt gttcaatcag ctgtccttcg gtattagctc
     9481 aaaaaacggc aaaatatgag gaactatttt tccgtttgtt tctctaatac tcaggacaaa
     9541 acgctgattt tacaacctcc tgataaacgg tgttcaaaag cttgcatctg cggtaataag
     9601 cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat gacttgatgc
     9661 aggacagctt tttcacaacc tctgatttct tattcttctt catttgtagc aaaataatta
     9721 cggtgataaa tgatacaaca tacactgata agaacaatgg cacctgcaaa ataaaacgca
     9781 tagctttcac caatatgttc accaatcaca cctgcaataa atggtgctaa agctccaccg
     9841 aaaaagcgga caaaactata gctggacgaa gcaacgtttc gttgcagacc tggtatttcc
     9901 atagcaatcg tcgtcaacag tgtattgata attccttgga aaaatcctgc taacacaatg
     9961 ctgatcggta caaggactgg catagaagtt ccagctccta ataacagcaa acaaataatg
    10021 aataagacaa atgctgctaa aattgtccga taagtcgtaa acttccgctc aagagccggt
    10081 gcaacgaaaa tcgaagcaat agccagaagc acaccccagc caaaaaatac aaaaccgact
    10141 tctaattcag aatagccaat caataaaaat ggcgcatacg caagtaaagt aaagaatccg
    10201 aaattatata acaatgcgat tagtccaact gaacgaagtt tatgattgct taacgctttg
    10261 acaccttccc aaaggcctac ttttctcttt ggtttctcag tttcaggtaa aagtaccgct
    10321 agggctataa aaccgcaaaa catcaaaaat gctaccccaa aaaatggata acgccatgaa
    10381 ccactgccca agaccccacc gactagaggg ccaacagcca ttccgcagcc aagtgctgct
    10441 tcatacatca tgattgcttt ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa
    10501 gcagtagaga caaataaagc attcccgaca ccccagccgg cacgtaaccc aatcagcatt
    10561 ccgatattcc cagataatcc gccaagacca gcgaatacaa cgatgataaa caatcctgtc
    10621 atcaatgttt ttttcaaacc gatccgacta gataagaaac ctgtaaatag catcaccaca
    10681 ccagtgacta acatgtaact agtaaataac agggtcgttt cagctggcgt agcgtttaac
    10741 tgtgcagcaa tcgtttttaa gattgggtct actagaccca ctcccataaa agcgatcata
    10801 caggtagctg ctacaactag cgctgaactt ttcccctttg tttcttcatt tgtttgttct
    10861 tgtatatcta tttgattctc caatatttcc atccctttct ctctattagc atcatacatg
    10921 atttatttta catgtcaata tgcacatgta aaacgtggaa actttactgt gatataataa
    10981 agaaaataag acgagaggta ggatttttat gaatacttta gcctatatat tattatgcgt
    11041 gcttttatcc aaaccacgtt ctgggtatga attgaagcag ctgatcacaa ttttttggga
    11101 agcccatcac agtcagatct atacaacgct tgccaagtta gaccagcagg gttatgtgac
    11161 cgttttggac aaagacgagc actctcaaaa aaagatctac cacttgactg atgaaggacg
    11221 tgctcttgta gaggaatgga tcaaagagga aactcccgct cctacgcaaa aagatgaatt
    11281 tctagcaaaa atctatgctt tttcgacgct cgataaaaca acagctactg gactgctatt
    11341 cactagagaa caacagctga acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct
    11401 ctcgctagac aagaaacaag aatttggacg ttatgttgtg atccagcgca gaattatgct
    11461 ttgtcagcag gaactccaat ggtgtcagca agtaagagaa caaatgcagc agttttttga
    11521 gtaagggata aacagaacct ttctagaaat atctattgaa gagcaaatta tcttcaataa
    11581 aagagcaata cagttggaaa agaaaaagcg atccgaacga caggccaaaa cctatcgttc
    11641 ggatcactat ttttctagaa ctgcattcaa gagactatga caaaaagcat gccaaggcct
    11701 tttttcaaat aacagtgttc catcattcat tactttgaat tgcttttagc acacttcttg
    11761 aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc aatcacattc attttccaag
    11821 cattcatata agcagagaat aattgatcag gtattccttg ttccatgatc acaccgaata
    11881 aagacataca tgtcaccatt cctaaaacca tcaagcatga aatagttaag atcatttgga
    11941 ccttgctttc tggatttact ggtagtttaa aggcaatttt tttcgccagc acaccgatga
    12001 tgaagacatc caaaatgaaa gccaccacga atccaggcac tagaccaatc aacaaacttg
    12061 ataaatgaag atcttgatgc agccataaat tatacgcgct catccccaag accatcaaaa
    12121 aacacatcat tgttgtaaaa attattcctt cttttttatt tgtaggcaat ctgtaccact
    12181 cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt aagtactttt
    12241 tttattttct gcccttttat ttcataaact tcatcagccg tacgctcgac aaagtaagaa
    12301 tcatgagaag taaacaaaat cgttcccgga tatatttgca ttagcttctc taaagcttcg
    12361 atcgttgcca tatcaataaa attagtcggt tcatctaaca gcaacacatt acttggctta
    12421 gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc cacttaatgt acataacggt
    12481 ttcgtcaaag tttcctgagc aaaccctaag tgattcagca aactacggac aagcgattct
    12541 gaaaactctg tttgtctcat gacaaaacga atgatggatt cttcagatgt catttgataa
    12601 cccttttgct gatatacttg aaagcttact ttcggagaga gaaggattcc ttctccttgt
    12661 ttgcggatat gttctaataa ggttgtctta cctgaaccat tttcaccgac aatcgcgatt
    12721 tttttaccta aagaaaactg aaaatcacca tttaccagta atgttctccc acttctttcc
    12781 aattggacat tttgtcccat gatgggatag cggctgtgga gttcaagaga ttttggtact
    12841 ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt
    12901 ctctctaatg ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag
    12961 gaagacaacc gatccagtct gatttgttgc tttttctttt tactggacac tttttccaac
    13021 ttttccgctt gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg
    13081 atcgactctg cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca
    13141 ctataattcc ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct
    13201 agctcatcta gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga
    13261 agctcttcaa tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga
    13321 agcatccctt gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct
    13381 ccgctccatt tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc
    13441 aaatttaggt cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc
    13501 tctgtttgga ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc
    13561 gatttaccga caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga
    13621 gaatcaatag atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact
    13681 gctaaatttt ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta
    13741 aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac
    13801 gaaataacta tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc
    13861 gaaaaaatgg acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt
    13921 tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc
    13981 ttttttatta ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga
    14041 aaacctttta ttcgaacata gtgatctttt ctggtgctaa acgaacagac tcgtaacctt
    14101 cttctaccgc ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac
    14161 caaatgcctc tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat
    14221 gatcacgcgc aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat
    14281 cgttcatttc ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat
    14341 ctctgacttc ttttggcatc ttccctgatt cgtaagcttg gttgtagatt tcttcgccat
    14401 attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc
    14461 tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca
    14521 caaagcgcca tggtttcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt
    14581 ccagcatttc ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac
    14641 cagtcataat tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct
    14701 gttgttatat tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt
    14761 tctacatcgc tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa
    14821 tgcttgattt tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga
    14881 ggatttctct gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact
    14941 gctccttgat caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct
    15001 ttcagtctca tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc
    15061 tggacgattt tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa
    15121 gacataattt cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa
    15181 taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa
    15241 agtatagaga aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac
    15301 gctaaaacca agattattga ttcttatcaa actatagaag atagctcttc tgcagtagat
    15361 aattttacac cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag
    15421 aaattggatc agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcacaacc
    15481 ttttttgagt aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa
    15541 aaacatgaaa atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc
    15601 actacctctt tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt
    15661 agccaatctc tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga
    15721 ttaaatagtc gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc
    15781 caaccgattt ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca
    15841 ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc
    15901 ccttcatatt cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat
    15961 aaatgttctc ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct
    16021 gtatcatact gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta
    16081 ggcgcagtgg cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg
    16141 cttttccatg caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc
    16201 gttacgttat cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc
    16261 gtaatccatt gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag
    16321 cttcctttaa tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag
    16381 ccactttcaa cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc
    16441 aaactttgtg cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag
    16501 cgagcataaa aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta
    16561 tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg
    16621 ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt
    16681 ggttcttcgg ttgaaattgt attttctgag acagtagctg actgcaaagt actttctgta
    16741 tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat
    16801 tcttcagcta gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat
    16861 aataaattct ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac
    16921 agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt
    16981 ttaataaatt tgctttttct gcataaaaag acgtaaatga agaatattct tctccattca
    17041 cgccttaaat tgatggtcgt ttgaataact atttaacaag taattctctc tggatattat
    17101 ccgttatgga ttatacttcc tgtgtcgtcg gacggataat catgttgctg acagacatcc
    17161 ggtctggcgt ttctatcgca aatgctacag cagaagctat atcttcagat gttaatcccc
    17221 attctttttg cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata
    17281 attcagtttg aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct
    17341 cttgacgtaa tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg
    17401 aatcaggata aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt
    17461 gtttttccat gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat
    17521 caagcatatt ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc
    17581 ctgcattatt gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg
    17641 cttggacttg tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta
    17701 aagattcttt tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc
    17761 ctttttctgc taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga
    17821 caatcacttt ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg
    17881 cttatactca ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga
    17941 cgattttggc agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg
    18001 cattcgctgc aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg
    18061 ctccgatcca agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt
    18121 ttggatcggt tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat
    18181 cttctaaaca gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt
    18241 tccctatact aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat
    18301 catctacttt gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt
    18361 acccagtatt cagcgctgct aaaggctctt cgttttccgc ttggatctga tggatcactt
    18421 caaagagcgg atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg
    18481 tcattttgct ccctcctttt atccttactt tataacggac ggcttatgat gtctaatact
    18541 tataaaacat acttaactat tccttttaac tatacttagt ggtatgcttt tttaaaaaca
    18601 ggagggaaaa aatggatatt cgtatactga cgtatttttt agcagtagct agagaaaaaa
    18661 atatcagccg agcagcaaaa tctctgcata tcacgcaacc tactctctca aaacaactga
    18721 aagatttaga agaagaactt ggtgtgacac ttttcactcg aggctcccga gagatacagt
    18781 tgacggattc tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa
    18841 aaacggcgaa caatttaaca aatgacgcaa tcatctctgg agaaatctac attggcggag
    18901 gcgaaactaa agcaatctct ttcatttctg atagccttca ttcgttgatc aaggcgcatc
    18961 ctgatatcca gtttcattta tatagcggga atgcagatga tatcatagac aaactggata
    19021 gcggattact tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat
    19081 ttcgactgcc agcccaagat cgttggggac ttttgataca tcggaatcat cctttagcaa
    19141 agcaaacagt catccatccg caggaactga aaactgttcc tttgatcatt tcccggcaaa
    19201 cgtcagtgga cagtcaacta gctgaatggc taggtttcag catagatcag ttatcgatcg
    19261 tcggaacata taacttattg tacaatgctt ctttgatggt cgataacggg acttttggtg
    19321 cattagctct agatgggatc atcaatactt atgggacgga aaaagtattc atcccactcg
    19381 atccgccatt acaagcttca ctgaatctca tttggaaaaa agaccagcct ctttctcctg
    19441 ctgctcgtaa atttttagat gtcctaacgg agaaatacag ttaagtagag tttttttcct
    19501 tcgctttcag ctttttaaca acgaataagt taagcaaaag atccaatgat taaataacat
    19561 aagtttctta atttgttcct tccttttatt tctgataatt ccagtaaatt actataatta
    19621 taaaaaaaga attgacaaaa cataactgtc tatataaaat tgattacgtt aacaataatt
    19681 cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta
    19741 ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa
    19801 ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt
    19861 attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag
    19921 aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga
    19981 aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc
    20041 agaaacgatc attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt
    20101 gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat
    20161 ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca
    20221 atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg
    20281 atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat
    20341 ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga
    20401 tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct
    20461 tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt
    20521 caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa
    20581 agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg
    20641 tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa
    20701 gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct
    20761 gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat
    20821 tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg
    20881 ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca
    20941 tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa
    21001 ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc
    21061 ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta
    21121 tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc
    21181 agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa
    21241 ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg
    21301 atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc
    21361 aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa
    21421 gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga
    21481 aatgcacgtc cagaaaacaa a
//
LOCUS       DANKHJ010000041        21152 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000041 DANKHJ010000000
VERSION     DANKHJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..772
                     /locus_tag="PW113_001720"
     CDS             <1..772
                     /locus_tag="PW113_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2526036.1"
                     /translation="QVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDSPPAF
                     LVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHVESYA
                     DKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGNIRVK
                     HFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFPGLGV
                     LKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            816..1328
                     /locus_tag="PW113_001721"
     CDS             816..1328
                     /locus_tag="PW113_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526037.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(1584..2132)
                     /locus_tag="PW113_001722"
     CDS             complement(1584..2132)
                     /locus_tag="PW113_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2526038.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(2221..3402)
                     /locus_tag="PW113_001723"
     CDS             complement(2221..3402)
                     /locus_tag="PW113_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2526039.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(3638..5146)
                     /locus_tag="PW113_001724"
     CDS             complement(3638..5146)
                     /locus_tag="PW113_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2526040.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            5305..5496
                     /locus_tag="PW113_001725"
     CDS             5305..5496
                     /locus_tag="PW113_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526041.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            5699..6616
                     /locus_tag="PW113_001726"
     CDS             5699..6616
                     /locus_tag="PW113_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2526042.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            6609..7682
                     /locus_tag="PW113_001727"
     CDS             6609..7682
                     /locus_tag="PW113_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526043.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            7830..8348
                     /locus_tag="PW113_001728"
     CDS             7830..8348
                     /locus_tag="PW113_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526044.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            8329..8721
                     /locus_tag="PW113_001729"
     CDS             8329..8721
                     /locus_tag="PW113_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2526045.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            8797..9732
                     /locus_tag="PW113_001730"
     CDS             8797..9732
                     /locus_tag="PW113_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2526046.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            9865..10644
                     /locus_tag="PW113_001731"
     CDS             9865..10644
                     /locus_tag="PW113_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2526047.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            10637..10954
                     /locus_tag="PW113_001732"
     CDS             10637..10954
                     /locus_tag="PW113_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2526048.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            11198..12808
                     /locus_tag="PW113_001733"
     CDS             11198..12808
                     /locus_tag="PW113_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2526049.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            12965..13813
                     /locus_tag="PW113_001734"
     CDS             12965..13813
                     /locus_tag="PW113_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2526050.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            14044..14523
                     /locus_tag="PW113_001735"
     CDS             14044..14523
                     /locus_tag="PW113_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526051.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            14556..16784
                     /locus_tag="PW113_001736"
     CDS             14556..16784
                     /locus_tag="PW113_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2526052.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            16913..18556
                     /locus_tag="PW113_001737"
     CDS             16913..18556
                     /locus_tag="PW113_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2526053.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(18629..19174)
                     /locus_tag="PW113_001738"
     CDS             complement(18629..19174)
                     /locus_tag="PW113_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2526054.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(19197..19682)
                     /locus_tag="PW113_001739"
     CDS             complement(19197..19682)
                     /locus_tag="PW113_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2526055.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(19672..20484)
                     /locus_tag="PW113_001740"
     CDS             complement(19672..20484)
                     /locus_tag="PW113_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2526056.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(20623..>21152)
                     /locus_tag="PW113_001741"
     CDS             complement(20623..>21152)
                     /locus_tag="PW113_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2526057.1"
                     /translation="RDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYK
                     EDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFL
                     AEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMK
                     VQRLQIEKELKSQKS"
BASE COUNT         6936 a         3880 c         4154 g         6182 t
ORIGIN      
        1 ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa
       61 tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc
      121 acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa
      181 acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg
      241 gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag
      301 ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg
      361 atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc
      421 tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa
      481 acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc
      541 aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg
      601 aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa
      661 aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga
      721 ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa
      781 tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag
      841 aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag
      901 gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg
      961 aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag
     1021 tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc
     1081 cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa
     1141 aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta
     1201 tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt
     1261 atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca
     1321 tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca
     1381 atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat
     1441 ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt
     1501 tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat
     1561 cctacgagta ttattcgata catttatatc aataatcctt tttttatttt atctgcagct
     1621 tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc
     1681 aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct
     1741 ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg
     1801 actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa
     1861 caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga
     1921 gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca
     1981 gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa
     2041 gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca
     2101 gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa
     2161 gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata
     2221 ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa
     2281 aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc
     2341 accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat
     2401 tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa
     2461 ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg
     2521 gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat
     2581 gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag
     2641 catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg
     2701 gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac
     2761 atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt
     2821 gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt
     2881 agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt
     2941 ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga
     3001 ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa
     3061 tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat
     3121 caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc
     3181 aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac
     3241 cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat
     3301 cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc
     3361 taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca
     3421 aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat
     3481 tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa
     3541 ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct
     3601 aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg
     3661 aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat
     3721 ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat
     3781 aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat
     3841 tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt
     3901 caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg
     3961 catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga
     4021 cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt
     4081 gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac
     4141 cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt
     4201 tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga
     4261 caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt
     4321 gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct
     4381 caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga
     4441 tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt
     4501 tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt
     4561 tttggctttc atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag
     4621 gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat
     4681 cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga
     4741 ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat
     4801 agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc
     4861 gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat
     4921 ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga
     4981 ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct
     5041 tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg
     5101 actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt
     5161 aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct
     5221 caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat
     5281 agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa
     5341 cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa
     5401 caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc
     5461 attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat
     5521 tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt
     5581 tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa
     5641 atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat
     5701 gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga
     5761 tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa
     5821 tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat
     5881 ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa
     5941 tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga
     6001 atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg
     6061 cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt
     6121 tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga
     6181 caaaaaaatc aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt
     6241 agatgaacaa ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt
     6301 cttgaatcgt aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact
     6361 atctaatgaa aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca
     6421 aaaagaagca ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa
     6481 agaacaaagg caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca
     6541 tgaattcttg atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg
     6601 ttccgagtat gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag
     6661 atcatgaaga aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag
     6721 aagaaatccc tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta actccgacga
     6781 atacgaatac tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag
     6841 aaggaaaaga gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat
     6901 tagcggattt acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat
     6961 catatgatcg ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga
     7021 tcgaatactt cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa
     7081 attcagctta tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac
     7141 aaaacgaatt agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat
     7201 cgatgagtct cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg
     7261 tgctttcttt gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga
     7321 tattcaatca caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta
     7381 atcagacatt actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat
     7441 tgacgaatga agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata
     7501 tggaaaagat caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga
     7561 taacaacaaa cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat
     7621 ctgtcattga tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct
     7681 gattggataa gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg
     7741 cagttatcca gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac
     7801 ttgctgatga aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct
     7861 tattgtctgc tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc
     7921 caatgactgc tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg
     7981 tcatgagtct ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg
     8041 gccaatggat cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa
     8101 cagtgcaaag gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg
     8161 gaataggtat gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt
     8221 accttcaagg ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg
     8281 tttttctgcc agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata
     8341 atttatagtt tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc
     8401 tatacacaat cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc
     8461 acgttacaag aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat
     8521 gatttactga gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact
     8581 tcgcttgaag ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac
     8641 gggaaaagtg ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt
     8701 ttcaatttgt ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa
     8761 ttcagtgagt tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga
     8821 atgttttccg taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc
     8881 ggctatgcaa tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat
     8941 tgcagcaatc agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag
     9001 ttgcatcaat ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg
     9061 cttttcttcg gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca
     9121 tcccatgaag tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc
     9181 gaaggaagtt cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt
     9241 tccacgatgc gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa
     9301 gacttgtgtg ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca
     9361 accgggattg ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga
     9421 gccgccattt tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca
     9481 agcgatttat caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat
     9541 ataaaaacag ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt
     9601 ggtaacaaag agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag
     9661 tctcttcctg acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat
     9721 tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt
     9781 ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt
     9841 gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct
     9901 ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta
     9961 ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt
    10021 tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg
    10081 acattattga tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc
    10141 ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat
    10201 gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag
    10261 gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat
    10321 tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg
    10381 ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc
    10441 tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca
    10501 gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa
    10561 gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa
    10621 caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat
    10681 cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc
    10741 ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa
    10801 agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat
    10861 gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg
    10921 acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag
    10981 attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg
    11041 gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt
    11101 tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag
    11161 atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg
    11221 aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat
    11281 tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa
    11341 tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc
    11401 gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat
    11461 ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta
    11521 tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata atgggatact
    11581 agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg
    11641 atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc
    11701 tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac
    11761 catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat
    11821 tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat
    11881 ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac
    11941 actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac
    12001 gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt
    12061 attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg
    12121 aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca
    12181 aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt
    12241 gactggaatg tctttattag ctggaccact atacacattg ttttttggct atgatcctga
    12301 aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat
    12361 cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga
    12421 agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg
    12481 gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat
    12541 gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt
    12601 ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg
    12661 attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg
    12721 cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt
    12781 taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa
    12841 aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc
    12901 taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa
    12961 acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa
    13021 ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga
    13081 aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc
    13141 tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa
    13201 agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac
    13261 attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat
    13321 cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca
    13381 gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct acgcttatct
    13441 gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct
    13501 gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt
    13561 attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag
    13621 aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga
    13681 agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat
    13741 tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat
    13801 ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatattccaa catcatcttg
    13861 gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc
    13921 aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt
    13981 agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa
    14041 ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat
    14101 aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct
    14161 cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa
    14221 aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta
    14281 gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca
    14341 gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat
    14401 accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac
    14461 ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat
    14521 taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa
    14581 accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga
    14641 caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa
    14701 tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca
    14761 aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg
    14821 acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc
    14881 aagaattact agaagctatt taccaaacag atacacttga agctgtactg acactgatcc
    14941 agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa
    15001 ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc
    15061 gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct
    15121 ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag
    15181 gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag
    15241 gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc
    15301 ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag
    15361 aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg
    15421 ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag
    15481 gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc actgattcag
    15541 atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg
    15601 acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca
    15661 ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct
    15721 tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag
    15781 aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg
    15841 aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg
    15901 acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca
    15961 tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa
    16021 aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc
    16081 ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg
    16141 aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa
    16201 agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt
    16261 caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat
    16321 tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa
    16381 gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg
    16441 aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag
    16501 cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg
    16561 gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg
    16621 ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca
    16681 aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg
    16741 aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac
    16801 gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt
    16861 ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca
    16921 agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga
    16981 tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa
    17041 agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa
    17101 tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt
    17161 tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat
    17221 caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc
    17281 gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac
    17341 atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca
    17401 tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact
    17461 attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat
    17521 ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt
    17581 tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta
    17641 cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt
    17701 agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca
    17761 agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac
    17821 ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc
    17881 cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc
    17941 tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat
    18001 gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta
    18061 taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac
    18121 aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc
    18181 tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca
    18241 agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac
    18301 caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta
    18361 tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc
    18421 acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa
    18481 ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata
    18541 taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt
    18601 tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt
    18661 taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga
    18721 caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg
    18781 cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag
    18841 aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt
    18901 ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta
    18961 gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt
    19021 ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa
    19081 caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca
    19141 attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa
    19201 tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt
    19261 ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat
    19321 aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct
    19381 aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg
    19441 tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga
    19501 ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag
    19561 atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt
    19621 aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc
    19681 atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat
    19741 cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt
    19801 aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc
    19861 gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata
    19921 gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt
    19981 tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag
    20041 agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg
    20101 aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt
    20161 gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg
    20221 aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt
    20281 cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat
    20341 aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc
    20401 aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc
    20461 gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt
    20521 ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa
    20581 aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt
    20641 taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc
    20701 aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg
    20761 attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt
    20821 atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat
    20881 agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg
    20941 gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc
    21001 ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat
    21061 atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt
    21121 catgtcttcc agtccaacgc ccatatctct tg
//
LOCUS       DANKHJ010000042        21075 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000042 DANKHJ010000000
VERSION     DANKHJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..138)
                     /locus_tag="PW113_001742"
     CDS             complement(<1..138)
                     /locus_tag="PW113_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2526058.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
     gene            complement(232..1281)
                     /locus_tag="PW113_001743"
     CDS             complement(232..1281)
                     /locus_tag="PW113_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2526059.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1363..2052)
                     /locus_tag="PW113_001744"
     CDS             complement(1363..2052)
                     /locus_tag="PW113_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2526060.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2158..2526)
                     /gene="rplL"
                     /locus_tag="PW113_001745"
     CDS             complement(2158..2526)
                     /gene="rplL"
                     /locus_tag="PW113_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2526061.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2579..3082)
                     /locus_tag="PW113_001746"
     CDS             complement(2579..3082)
                     /locus_tag="PW113_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2526062.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3286..3413)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3492..4181)
                     /gene="rplA"
                     /locus_tag="PW113_001747"
     CDS             complement(3492..4181)
                     /gene="rplA"
                     /locus_tag="PW113_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2526063.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4285..4707)
                     /gene="rplK"
                     /locus_tag="PW113_001748"
     CDS             complement(4285..4707)
                     /gene="rplK"
                     /locus_tag="PW113_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2526064.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            5050..5718
                     /gene="sdaAB"
                     /locus_tag="PW113_001749"
     CDS             5050..5718
                     /gene="sdaAB"
                     /locus_tag="PW113_001749"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2526065.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5731..6603
                     /gene="sdaAA"
                     /locus_tag="PW113_001750"
     CDS             5731..6603
                     /gene="sdaAA"
                     /locus_tag="PW113_001750"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2526066.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6694..7713)
                     /locus_tag="PW113_001751"
     CDS             complement(6694..7713)
                     /locus_tag="PW113_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2526067.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7886..8791)
                     /locus_tag="PW113_001752"
     CDS             complement(7886..8791)
                     /locus_tag="PW113_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2526068.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8850..9395)
                     /gene="nusG"
                     /locus_tag="PW113_001753"
     CDS             complement(8850..9395)
                     /gene="nusG"
                     /locus_tag="PW113_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2526069.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9496..9666)
                     /gene="secE"
                     /locus_tag="PW113_001754"
     CDS             complement(9496..9666)
                     /gene="secE"
                     /locus_tag="PW113_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2526070.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9690..9842)
                     /gene="rpmG"
                     /locus_tag="PW113_001755"
     CDS             complement(9690..9842)
                     /gene="rpmG"
                     /locus_tag="PW113_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2526071.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            10004..10648
                     /locus_tag="PW113_001756"
     CDS             10004..10648
                     /locus_tag="PW113_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2526072.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10704..11207)
                     /locus_tag="PW113_001757"
     CDS             complement(10704..11207)
                     /locus_tag="PW113_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2526073.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11220..11266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11804..12769
                     /locus_tag="PW113_001758"
     CDS             11804..12769
                     /locus_tag="PW113_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2526074.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12868..14040)
                     /locus_tag="PW113_001759"
     CDS             complement(12868..14040)
                     /locus_tag="PW113_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526075.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(14027..15172)
                     /locus_tag="PW113_001760"
     CDS             complement(14027..15172)
                     /locus_tag="PW113_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526076.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15186..16118)
                     /locus_tag="PW113_001761"
     CDS             complement(15186..16118)
                     /locus_tag="PW113_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526077.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16235..17314
                     /locus_tag="PW113_001762"
     CDS             16235..17314
                     /locus_tag="PW113_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2526078.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17311..17929
                     /locus_tag="PW113_001763"
                     /pseudo
     CDS             17311..17929
                     /locus_tag="PW113_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(18039..18266)
                     /locus_tag="PW113_001764"
     CDS             complement(18039..18266)
                     /locus_tag="PW113_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2526079.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18369..20957)
                     /locus_tag="PW113_001765"
     CDS             complement(18369..20957)
                     /locus_tag="PW113_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2526080.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20977..>21075)
                     /locus_tag="PW113_001766"
     CDS             complement(20977..>21075)
                     /locus_tag="PW113_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2526081.1"
                     /translation="ETEEPCRLLPYELKVWNQTDFLGTETAPEPLL"
BASE COUNT         6184 a         4422 c         3792 g         6677 t
ORIGIN      
        1 cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat
       61 gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt
      121 atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac tactatatgc
      181 gtttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      241 accacgggta aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg
      301 tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag
      361 caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac
      421 aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg
      481 tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca
      541 attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc
      601 aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg
      661 cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg
      721 cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata
      781 gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat
      841 ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc
      901 tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa
      961 agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc
     1021 agataacgct accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac
     1081 taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac
     1141 acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat
     1201 attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg
     1261 tgttttttca ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg
     1321 aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt
     1381 tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac
     1441 acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca
     1501 cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag
     1561 aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat
     1621 atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg
     1681 attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat
     1741 gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa
     1801 accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt
     1861 caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc
     1921 tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa
     1981 ccgatagccc actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat
     2041 tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata
     2101 aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta
     2161 tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc
     2221 tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt
     2281 caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc
     2341 tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc
     2401 agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag
     2461 gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa
     2521 tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt
     2581 aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga
     2641 ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg
     2701 ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt
     2761 ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt
     2821 cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag
     2881 cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat
     2941 cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga
     3001 ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt
     3061 ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt
     3121 tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca
     3181 cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct
     3241 ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa
     3301 actctatgcc accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta
     3361 ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact
     3421 agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt
     3481 ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg
     3541 atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca
     3601 tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt
     3661 ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg
     3721 tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct
     3781 tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca
     3841 actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg
     3901 aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca
     3961 gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct
     4021 tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg
     4081 tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact
     4141 ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg
     4201 tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga
     4261 agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc
     4321 ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc
     4381 agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc
     4441 tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt
     4501 aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc
     4561 ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg
     4621 acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt
     4681 aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta
     4741 attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata
     4801 atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt
     4861 tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa
     4921 tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa
     4981 agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag
     5041 gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg
     5101 attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt
     5161 atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc
     5221 tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat
     5281 gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt
     5341 gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga
     5401 acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa
     5461 ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa
     5521 gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc
     5581 ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta
     5641 gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc
     5701 gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat
     5761 caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt
     5821 ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa
     5881 tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca
     5941 aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa
     6001 gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct
     6061 acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg
     6121 cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta
     6181 gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt
     6241 tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa
     6301 gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt
     6361 gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt
     6421 atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt
     6481 gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt
     6541 ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg
     6601 tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa
     6661 gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac
     6721 ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg
     6781 ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg
     6841 gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg
     6901 gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa
     6961 tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat
     7021 accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac
     7081 gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg
     7141 ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg
     7201 ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc
     7261 ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc
     7321 ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg
     7381 gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata
     7441 agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc
     7501 tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc
     7561 aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat
     7621 gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc
     7681 accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta
     7741 ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga
     7801 tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa
     7861 catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat
     7921 ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt
     7981 cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg
     8041 gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag
     8101 tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag
     8161 caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa
     8221 aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg
     8281 ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat
     8341 ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc
     8401 actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag
     8461 acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta
     8521 ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc
     8581 tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg
     8641 ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta
     8701 acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga
     8761 ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa
     8821 tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca
     8881 agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct
     8941 ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg
     9001 acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag
     9061 atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca
     9121 acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc
     9181 atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt
     9241 ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc
     9301 atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta
     9361 tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa
     9421 ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac
     9481 gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat
     9541 tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg tatctttacg
     9601 gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa
     9661 tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga
     9721 ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact
     9781 gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc
     9841 atttacttct gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact
     9901 ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat
     9961 taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact
    10021 tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt
    10081 aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat
    10141 tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat
    10201 gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc
    10261 tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga
    10321 aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg
    10381 gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt
    10441 ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga
    10501 tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc
    10561 gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat
    10621 tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg
    10681 ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa
    10741 taatacatca atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca
    10801 taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg
    10861 ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt
    10921 tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc
    10981 atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc
    11041 aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc
    11101 cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca
    11161 tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt
    11221 ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat
    11281 aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag
    11341 cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg
    11401 gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga
    11461 aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct
    11521 agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt
    11581 aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt
    11641 tcctagactt ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac
    11701 aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag
    11761 gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca
    11821 tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt
    11881 accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca
    11941 cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga
    12001 acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca
    12061 tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt atattccagc
    12121 aggttctgga aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact
    12181 tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa
    12241 aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat
    12301 cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc
    12361 attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct
    12421 ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa
    12481 gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt
    12541 cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt
    12601 acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt
    12661 acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg
    12721 accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt
    12781 ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt
    12841 tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata
    12901 gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa
    12961 gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact
    13021 gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac
    13081 catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat
    13141 aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc
    13201 cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt
    13261 tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa
    13321 cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat
    13381 gggcgcactc tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct
    13441 gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt
    13501 caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga
    13561 gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag
    13621 aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc
    13681 tgggcgactc tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc
    13741 tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt
    13801 ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc
    13861 atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt
    13921 attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc
    13981 tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat
    14041 aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca
    14101 accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac
    14161 tttcgctgat caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc
    14221 aatacttcac ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg
    14281 aaatcaagat gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg
    14341 tacctaacaa aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac
    14401 gttcaccaaa gtctacttga tagataaaac gaaagacagc taacataata taaatctctg
    14461 tcaaaacaat cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag
    14521 gaatcaagga caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc
    14581 ggacccccca catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac
    14641 ttttgaaact catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa
    14701 cagtcatgat gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac
    14761 caatacgctg taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt
    14821 gacccacatg gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg
    14881 catttagctt ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat
    14941 cattgacttc gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg
    15001 ctttgattac aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa
    15061 aggccgccat aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg
    15121 atttatttcg cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc
    15181 ctcctctagt ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct
    15241 tctttggttg ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc
    15301 aacaatactg tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt
    15361 ccatcagaag ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg
    15421 tcgatcgtca aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta
    15481 gcaatcacct gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc
    15541 atgtaatgtg ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct
    15601 aaaattttat tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac
    15661 cgcggcttgt gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt
    15721 tctcctggac gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc
    15781 aaggctttcc ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct
    15841 tgcacactta gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg
    15901 acagcataat tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa
    15961 tttaacaaag aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac
    16021 agacctaaga cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct
    16081 ccatagcgtt tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt
    16141 tcttgttgat ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc
    16201 acacatgaca tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga
    16261 aactagcttt ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat
    16321 gcaagttatc tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac
    16381 aaagccaaat tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct
    16441 tgtcctgttt ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta
    16501 cttgtttttg ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt
    16561 cgcctttgtc gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac
    16621 gaccgatctt gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga
    16681 gttatcggaa caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat
    16741 tgtggaagaa cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc
    16801 aagtgcgatt ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga
    16861 accattagaa aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt
    16921 ccatgacctc catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga
    16981 ttttccttct tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca
    17041 aaactgcttc ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc
    17101 caaaaccgtt gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa
    17161 tgatggcacc agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca
    17221 acgtgttcaa gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt
    17281 aacgatcatt ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat
    17341 ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta
    17401 ggcattggtc attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga
    17461 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17521 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17581 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17641 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17701 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17761 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17821 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17881 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17941 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    18001 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    18061 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    18121 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    18181 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18241 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18301 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18361 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18421 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18481 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18541 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18601 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18661 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18721 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18781 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18841 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18901 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18961 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    19021 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    19081 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    19141 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    19201 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19261 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19321 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19381 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19441 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19501 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19561 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19621 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19681 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19741 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19801 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19861 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19921 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19981 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    20041 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    20101 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    20161 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    20221 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20281 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20341 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20401 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20461 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20521 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20581 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20641 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20701 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20761 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20821 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20881 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20941 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    21001 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    21061 aggctcttca gtctc
//
LOCUS       DANKHJ010000043        20976 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000043 DANKHJ010000000
VERSION     DANKHJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            305..646
                     /locus_tag="PW113_001767"
     CDS             305..646
                     /locus_tag="PW113_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526082.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(639..830)
                     /locus_tag="PW113_001768"
     CDS             complement(639..830)
                     /locus_tag="PW113_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDL2526083.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            1180..1392
                     /locus_tag="PW113_001769"
     CDS             1180..1392
                     /locus_tag="PW113_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526084.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            1399..1623
                     /locus_tag="PW113_001770"
     CDS             1399..1623
                     /locus_tag="PW113_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526085.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            1650..2183
                     /locus_tag="PW113_001771"
     CDS             1650..2183
                     /locus_tag="PW113_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL2526086.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            2170..3414
                     /locus_tag="PW113_001772"
     CDS             2170..3414
                     /locus_tag="PW113_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL2526087.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            3427..4926
                     /locus_tag="PW113_001773"
     CDS             3427..4926
                     /locus_tag="PW113_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2526088.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            4931..5857
                     /locus_tag="PW113_001774"
     CDS             4931..5857
                     /locus_tag="PW113_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2526089.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            5984..6586
                     /locus_tag="PW113_001775"
     CDS             5984..6586
                     /locus_tag="PW113_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2526090.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            6599..7522
                     /locus_tag="PW113_001776"
     CDS             6599..7522
                     /locus_tag="PW113_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2526091.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            7540..7803
                     /locus_tag="PW113_001777"
     CDS             7540..7803
                     /locus_tag="PW113_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526092.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            7815..8144
                     /locus_tag="PW113_001778"
     CDS             7815..8144
                     /locus_tag="PW113_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDL2526093.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            8141..8467
                     /locus_tag="PW113_001779"
     CDS             8141..8467
                     /locus_tag="PW113_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526094.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            8451..8789
                     /locus_tag="PW113_001780"
     CDS             8451..8789
                     /locus_tag="PW113_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2526095.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            8789..9181
                     /locus_tag="PW113_001781"
     CDS             8789..9181
                     /locus_tag="PW113_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDL2526096.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            9192..9704
                     /locus_tag="PW113_001782"
     CDS             9192..9704
                     /locus_tag="PW113_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDL2526097.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            9750..10118
                     /locus_tag="PW113_001783"
     CDS             9750..10118
                     /locus_tag="PW113_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDL2526098.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            10172..10459
                     /locus_tag="PW113_001784"
     CDS             10172..10459
                     /locus_tag="PW113_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526099.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            10476..13595
                     /locus_tag="PW113_001785"
     CDS             10476..13595
                     /locus_tag="PW113_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2526100.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            13628..14395
                     /locus_tag="PW113_001786"
     CDS             13628..14395
                     /locus_tag="PW113_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDL2526101.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            14392..17223
                     /locus_tag="PW113_001787"
     CDS             14392..17223
                     /locus_tag="PW113_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2526102.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            17198..18955
                     /locus_tag="PW113_001788"
     CDS             17198..18955
                     /locus_tag="PW113_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL2526103.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            18972..19754
                     /locus_tag="PW113_001789"
     CDS             18972..19754
                     /locus_tag="PW113_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526104.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            19754..20755
                     /locus_tag="PW113_001790"
                     /pseudo
     CDS             19754..20755
                     /locus_tag="PW113_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7337 a         3374 c         4557 g         5708 t
ORIGIN      
        1 aagtagccag tttttacact ttttataccc tttgattacc catttggttt ccattttata
       61 ccttttttat accaatcact tacctggtaa atgatttatt atgatagtgt cgaaagatta
      121 gtgataggtc tgagacaaaa taataataaa aggaacatcg ttttattatt gtttcacaat
      181 taagcttcga tagacagcaa cggaaatatt aagaataagg atgtgaattc caactccttc
      241 taaattgttc ttattatcta tcatccgttg ctgtttatta atttatgtat tggagggaaa
      301 acgaatggaa gtcgagcaaa taaagtatca aggtatggat cgggaacggt tgattgatat
      361 tattgagcaa caacgtttag aattgatcca aaaagaagct gtatgtaaag agtacaaaaa
      421 acatttagaa caagtaattg agtatcattc agtagaaaag tacagatcag tggtgcaaaa
      481 aaatagagca acgagtcctg atcctcgtca agatgtatcg attagtatca agacgccagt
      541 atttaattct gttaaatcag aaaagctaga aagtatctca actgctttgg tagaaaaagc
      601 agctgaaatg aactccctag caaatgaagt tagtcgtctt atttagtcac atcgataact
      661 tgagagtctt tggtgattcc gttttttata gaattaatgg tttgttcagc ggaatattta
      721 gtcaagtatg tttcgctagt tgctactact tcattgttat cggacttaat aacgaagtaa
      781 tattggccat tagttgcttc tcttataaca aagtacaaat ttttcaccac cttttaattt
      841 atttcagcgg accactcgct gataaataaa attatacact tagtatttat tttcacaata
      901 ttaatttgtc actgtggcgg aaagggtaga cgctaagcat gtgtgttagg tcaatgcttc
      961 ggcaaccatg caaggttcga ttccttgcca gtgactttgg caactgaggg ttggaaatgg
     1021 gcgctcaaag tacacgagca aggcgaggtc gatagtaatc gatggaatcg gtgtaggttg
     1081 cttgatagaa ctataactgc atctcgttta tgaggtgtag tttttacata ttagatcact
     1141 catcgagtgg tctttttatt ttggaagggg agtaaaaaaa tgaacgaaaa ccaattaaga
     1201 gagttgttta aaacgaatga agcaaacaaa acaatggagg cgacattcta cgaaactcaa
     1261 aaaagcttag cgttaatcgc aaaacaagct aagtatttct acgatcagct tattctgcaa
     1321 ggatttaatg acgcacaggc tatggaattt atgatgcgaa ccttttctgc cagtaaccaa
     1381 cagaaagagt gatacgtaat gaggaactac tggtatgtat cattatcaaa taaatatccg
     1441 caaccaaacg cagatgatcc aattagagtt gtccagtcag tccagattaa aaagaagtac
     1501 tccatcattg aaatgaccag agaagctacg ccaaaagaga ttgataagta caatcttcgt
     1561 tactgcggcc atggatattt tagtgagcag aacatacaga caaatattga gagatattgt
     1621 taataaaatt gaaaggtggt ggcttgaatg tggtaaattt gacaccaaaa caaaaagctt
     1681 ttgcggatga atatataaaa aatggtggta atgctactca agccgccatt aaagcaggtt
     1741 atagcaaacg atcagcaaga gtaataggta aggaaaacct aactaaacct aacataatac
     1801 agtatattaa tgaacggcta aatcctatcg aaaagaagcg caaattaagt gccgaggacg
     1861 ctttaaatga attgatagat atttggcaag gagaagtaca aataagcgtg agcaagcaaa
     1921 tagaccgctt ggagaaaaac aaggttatta aacatatgca atatgaatat acaccagatt
     1981 tagaaagcaa attgaaagcc ttggatttgt atttgaagta taaatcgctg ttatcacaaa
     2041 cgcaattaga aaaagctcaa acagaaataa aattaatgca agcaaaatta gaacaattac
     2101 agataaactc agagcgttct accgaagaaa aacttgatga gttgttagaa aagattagtg
     2161 gtgaattaga tggcactagt tgatatttat aacccaaagc aaatcgacgt gttaaataaa
     2221 accattaaga atgattggtt tattacttta ttacatggag caaaacgttc tggaaaaaca
     2281 aaaataaaca atgatttgtt cttgtttgaa ttgcgacgtg tgcgaaagat tgccgatgaa
     2341 gaagacatta aggaaccaat gtatatccta gcaggagttt caagtgcaac aatccaaaag
     2401 aacatcttac aggaactata caacatgtac agcatagaac ctaaattcga taaacatgga
     2461 aactttaagc tatttggcgt taaggtcgta caagcttata caggaaacat tggcggtgtt
     2521 ggcgcaattc gtggtatgac agcatatggc gcttatatca atgaggcatc gctagctaga
     2581 caagaagtat ttgctgaaat cgtttcacgt tgttcaggaa caggagcgag aatactagct
     2641 gatacaaacc ctgacaaccc ggaacactgg ctaaagaaag agtatatcga caattcaagc
     2701 aaaaatattc aatcgttcca ctttgaatta gatgataata catttttatc tgaacgatat
     2761 cgtaacaata ttaaagaatc aacaccaagc ggcatgtttt atgatcgtga tatcaaaggt
     2821 ttatgggttt ctgcagaagg tgtcgtttat cgtgattttg atgctagcaa gcactacatc
     2881 cagtcaaaag acttaccacc tttgagcaac ttttattgtg gtgttgactg gggatatgaa
     2941 cactggggtt caatcgtagt tataggtgaa acggatgacg gaacagctta tttaatcgaa
     3001 gaacatgcta ctcaatttga agagattgat tattgggtag atgtggcaaa aggcattcaa
     3061 gagcgttacg gcttacgagt acctttttat tgtgactctg cgagaccgga gcatgtggct
     3121 aggtttgtaa gagaaaagat taatgctaaa aatgctcata aagcacggtt atctggagtt
     3181 gaggaagtcg ccaagagatt taaacaagat aaattgttta tctgtcaaga tagagtgatg
     3241 aaatttcggg atgaaattta tcaatacatt tgggacaaga aaaaaggcga gccaataaaa
     3301 gaatatgatg atgtgctaga ctccgttcga tatgcgatat atactcatga gcttcttaag
     3361 aaaccaaaag ttaatgtcaa cgaaaagatt aaacgtatta aacgcatgtt ttaaggagtg
     3421 tgaaaaatgg ataaggtaaa cgagtttgaa tacggagctg atatacatta ttctaatgac
     3481 gtgaacacaa attatgtaaa gtttagcgta gactcaaatc ttcactatag atttagctca
     3541 gcagaagatt tacttaacga tttagatact ttagcagcaa tgataaaaca tcatcatgaa
     3601 tatcaggtaa aaaggctaag tgtattagat gattattata aagctagaaa tacaaatatc
     3661 atggataacc gtaggcgcag agaaaaagaa aaagcggatc accgatcagc acataacttt
     3721 ggaaaagttc tttgtacgtt tgatgttggg tacaacacag gcaatcctat aaaagtgcaa
     3781 atcgaggaca caaatcaaca aaaagaaatc gaagagttta atactaataa tgacatagat
     3841 gggttaaatg ctgaactctg gcttgatatg gataagtatg ggagagccta tgagattatc
     3901 tatcgagatt cagatgatac agattatgtt gatttggcta atgtatttga aacgtttgtt
     3961 gtatatgata ctacagtaaa acgagagcct attttggctg tacggtatcc taagacaaga
     4021 ttcaacaagg atgctgataa acagtacatt caaccaatcg tatacacaaa agaaaaaagt
     4081 atcacttatg atgagacgac actaacagca attgagttaa agaatcccca ggatgaaccg
     4141 catgaatata aagaggtacc tattacagag tattctccta atcgttttcg gatgggcttg
     4201 tatgaagatg tactatcttt gattgatcta tacgatgcag ggcagtctga taccgccaat
     4261 tatatgactg atctaaacga tgctcttcta gttattagtg gtgatattga agcagcagga
     4321 ctatccacag aggacgccat caagcagaaa gaagcgaata tgcttttgct tgagtctgga
     4381 actgatgtga acggtaataa aacaagtgtg actgcaggat atatttacaa acaatatgat
     4441 gtgaacggtg tagaagcata caaatacaga gtacgcaagg atattcacga aatatccatg
     4501 gttcctgatc ttactgatga caatttttcc ggagtgcaat cgggagaagc aatgaaatat
     4561 aaattatttg gatttgaaca aatgacggca acaaagcaaa ggctattcaa aaaaggcctt
     4621 atgcggcgtt atcgtctttt atttagccta aaatcaagta tttctgaaat ggataactcc
     4681 gatttgaaag gcttacgtgt aatatttacg cctaatctac ctaaagccat tctggaagag
     4741 ttgaaatctt tggttgatgc tggagctgaa ctcagtcaag agacgatctt aggactcgct
     4801 tcttttgttc cagatgtaca ggcagagttg aaacgagtaa ataaagaaac gcaaaagcag
     4861 attggcattt ttgattcaga tggtgaagaa gtaattaaca acaaaaaaga tgaaacaggg
     4921 gggtgattaa atgaactccc aagaatattg gatcaaacgg gaaaaggaat ggcaaaagca
     4981 acaaattaaa gatgataaaa agcgcatggc agaaattaaa agtcgcatgc aatacgcaca
     5041 agatgcgata caaaaagaaa tagacgcgca gtgggacagt ttctccaatg gccagaaaat
     5101 cactcgtagc gaagcgatga agcgtgctag tgaaatggat gtcaaagcat tcgctcgcaa
     5161 agcaaagaag tatgtcaaag agaaagattt ttctcctaca gcaaaccaag aattaaagct
     5221 atacaatctt acgatgcgtg taaatagatt agagctctta aaagctaata tcgggcttga
     5281 attgatttca ctgtttaatg aattggataa gtacttttcg aatgaattaa caaaagctgg
     5341 tttagctgaa ttgaagagac aagccggtat tttagaaatg actattgctt caagtggata
     5401 tgcaaagctg atagaactag taataaacag ctcctttttg agtgatgacg tgtcttttag
     5461 tgatcgctta tggatgtatc aatctgaatt gaaatcagaa ttagataggt tgttagtcag
     5521 aagtataacg atggggaaaa atcccaagca acttgcatct aaattggcag aatatttaac
     5581 agctgaagga cgagaaaaca ctaagttcaa cactcaacgt ttgatggtga ctgaaacgac
     5641 tagagttcag gtagggattc aagaacgaag ttatagagat gcaggcatta cccagtacat
     5701 ctatatagca gaaccaacgg cgtgcaaact atgtataccg ttaaataatc aagtttttga
     5761 tgttgccgat atgcagccag gaagtaacgc tcctaatatg catccatttt gtcgatgcag
     5821 tacagcacct tatatagaac gaatatcaag ccgttaacac aaattaacgg ctttttattg
     5881 tgccttctta cagcttacag gcgttaaaga gaaagctatt ttcggctgac cggcgtaact
     5941 ggtcaaattt atcgggtagc ggcgtaaccg tggaggatta atcatgaaaa aacgtttatt
     6001 tatgccaatg aacttacaat ttttttctga accaggagat ggtggatctg gtgatgaggg
     6061 acaacaagga aacctaccag ctggctcaca agagacaccg cccgaagcaa aagaagaaaa
     6121 caatactggc aaaacatttt ctcgtgatga agtagcgaaa atgatcgctg ctgagacgaa
     6181 taaagcaaaa gcagcgtggg aaaaagaact agcagcaaaa aaagaagaag ctaaaaagct
     6241 ggcaaaaatg aatgcggaag aaaaactaca gcatgagttg gagcaaaaag aagctgaaat
     6301 cgctgaatta aagcgtggac aggcactatc tgaaatgacg aaagaagctt ctaaaatgct
     6361 gacagatgca aatttaccac acgatgatga tttacttggt ctgattgttt ctgatgatgc
     6421 agatgccaca aaacaagctg tagcagtcat cactaacttt gcttctttga ttaagagaga
     6481 aaacgcaaga caaacaccac caaatgaagg tggacaattt acagcatcga aaaatactaa
     6541 agaaacagtg gctaaactag ctgctaaaaa tcgaattatc aaataggagg aaaacttaat
     6601 gaaaaagaaa caacttttac caatgaactt gcaaatgttt gctcaaacat gggatccaga
     6661 taatgtcttg gtatatgaaa cgaaagaggg aaaaattcct gataaatata atacgctcat
     6721 tttgagtgaa gttatggaaa attctaagat catgcagtta gcaaaatacg aagaaatgac
     6781 tgacaaagaa aagaaatttg aatactttgc agaaggacca ggcgcatact gggtgggtga
     6841 aggtgaaaaa attaaaacgt ctaaacctaa atggatgcaa gctacgatga ctgcaaaaaa
     6901 actcggtgtc attcttccgg tttctcgtga atatttaaat tataaattat cagatttctt
     6961 tgaggagatg cagccaaaaa ttgctgaagc tttctataaa aaatttgatg cagctgcctt
     7021 attaaataaa gaaaacccat ttcctcagtc actagacgga tcagttatta gtgcggggaa
     7081 tgtggttgaa ggcggattga cttatgataa tatcctagcc ttagaagaca agttagcaga
     7141 aaatgaattc gaacctaatg cgtttatttc aaaccgaaaa aatcgtacag aattacgttc
     7201 tgcagctcaa acagtcgggt caaatgttga gtttatttat gatcgctcta ctaatacaat
     7261 tgacggatta ccagtagtag accttaagtc tttagataaa ggaacacttt acgctggaga
     7321 ctttaattac atgttttatg ggatcccata taatatttca tttaagattt ctgaagaagc
     7381 ccaattgtct actttaacta atgaagatgg aaccccagtt aacttgtttg agcaagaact
     7441 gattgctttg cgtgcaacaa tggatgttgg atttatgatt gtaaaagatg aagcatttgg
     7501 gaagatttcc ccaaaagcgt aacgcctgct accggtattg tgccaaatca aaagacatgg
     7561 accggtaaag taggcgatac taaaacattt actatttcag ctgtgcctgc agatgctagc
     7621 gatgcagctg ctgttgttgc agctactaca gcaacttcaa gtgatggagc tatcgcaaca
     7681 gtgaccaaaa atgaaaatgg tggttttgat ggaacgattg cagcagaagg gtcagcaaca
     7741 ttcacattta cttctggaga attcactact tcaatcaatg tgacaggtca acctgctagt
     7801 taggaagtaa aaatatgatg attgcagatg atattaaaaa acttcttaaa ggaacaatag
     7861 atgaaaagct tgaagttatt gagcgaagaa cgaatgagcg tatgaaaacc ttgttaaata
     7921 cgcaagaagt tcctaaagaa tttgaaacag ttgtatatga agtatcgttg aaaagattca
     7981 atagaattgg tcaagaaggt atgcagtcat attctcaaga aggtttatct atggcttttc
     8041 ctgactcgga tttttcagag tatcaaaatg agattgatga atttaagcgt aaagatcagg
     8101 aagagttgta caagccaaag cgagggaggt ttaaatttgt atgagattta cagatgaaat
     8161 tatatttgtt aaacgttcat ctgactctaa atatgatcca gatctcggtg agtgggttga
     8221 aggcaaacca gaaagaacaa gaacagaggc aaacgtgaca gatattggca ctgatagaag
     8281 tgtgactatt tttggtagtg tggaagaagg ggcgaaggtc attaggacgc agcctctttt
     8341 ttctatccct acatttgact atatcgagat tgaaggaaag acttggcaac aaaaaacagc
     8401 tagaaatcca gcatatagaa atagtttaat tgtgcaagag gtggttcttg atgaaggcac
     8461 aacttgaata taaaggaatc gatcagctga tgcgacatct gaaaaaagca gcaacgctta
     8521 atgacgttca aaaagtcgtg aaaagtaata ctgctgaaat gactgaacga atgcaaaaag
     8581 gtgcgccagt ggatacaggt tacttacgaa gatcaataaa catgaatctt ttagaagctg
     8641 gtttaactgg tattgtagga ccgacagcag attatgcacc ttatgtagaa tatggcactc
     8701 gatttatgtc ggcacagcct tatgttagac cagcgtttaa ttaccaaaaa gtcaaattta
     8761 tggctgaaat gaaagccttg gtgaaatgat gattaagaca agagatcaat caatttttga
     8821 tgaacttttt aaaatatccc aaaacaaact tggatataaa acatacgatt acaaaacttt
     8881 agaggatgtt ggttatcctt ttgtggaatt tgagaacact cagacaatcc atgaagtaaa
     8941 taaaactgac attaaagggt ctgtgattgt ggttttatcc gtttggggat tacagaagaa
     9001 acgaaagcag ttgtcagata tggcatctgc tctttttaat gaagctagat tgatagaagc
     9061 cacagaaggc tattattggg ctttaaatta tcaagcaagt ggaattcaag tgatggacga
     9121 cacaacaacg aatacaccgc taaaacgggc ggttgtcaca cttgaattta gaattagata
     9181 ggaggaagaa catggaagca ttaaaaggta ttgatgtcat tttgctttat cgcttattga
     9241 aaaaagaaac tcaggaagcc gcttggaaaa tggcatttca aacagaacac gaaaatggtt
     9301 tatcaagaga ttcagactct acagtgacaa aagatggaaa cgttcaaagt ttaagcccgg
     9361 ttgaatatga tttttcggct acttcaatag ttgctaaagg agattctcat gtagatgaaa
     9421 tgaaacaagc tttattaaat ggggatatca ttgaaatttg ggaaattaat aaagcagaac
     9481 agggaacaga tgataatgca aataagtaca aagctactta ttaccaagca tatgtgtctg
     9541 aatttactcc atcggctgct gcagaggata acgttgaatt aagtttatca tttgcagtaa
     9601 atggtgttgg tcaagatggt tatgcaacct tgacagaaga tcaagccgat gttgttcaat
     9661 atgcattcaa agataccgtg aaagcaactt cgacaggagc ataagagggc ttagatgctc
     9721 tcttttttat tttaggagga tgaaaaacat tgaaattaaa aattaaaggt aaagaatatt
     9781 cgtttaaatt tggcactaaa tttgtacgtg aattagataa agtgatgcct ttcgtcgatg
     9841 gaaatatgga atttggaatg ggactctccg caaaagtctt accagagcta cgttcttata
     9901 acgttaacac gttatcacga gttctggaaa tagcaaatag aactgaagat gaatctatta
     9961 cattagatga attggatgat tacatcgatg aagttaaaga cattgaaaaa ttgtttgatg
    10021 aagtcctaaa agaattggcg gagtcgaacg cgggaaagtt agcggtccga aacctgaatc
    10081 agaaattgaa agaagcggaa aaacaacaag tggaatagat tctgcactgg catacgaaca
    10141 aattcttatc aattcttttc gatatttggg aatgaccaat atctcagata tcgaaagaat
    10201 gacgttatat gaatacaaca ttcgtatgac tgcagcccag ttatcttggc ttgacaaaga
    10261 aaagttgatt cacgaattag cgtgggcaaa tcagcaagtc caagcggaga aaaaagtagg
    10321 caaaaagaca gttcctgtat atcgatcctt tgaagaattc ttcaattatc aaaaaatcga
    10381 agattcaatc atgggagttt ccgaactttc aaaacaagat aaaaaattcc aaagcttact
    10441 aactaaagct aactcttgag gaaaggagga gaatcatgga acaattttct gttgaagcct
    10501 tattaaaagc cacagatagt ggatttgtaa agacttttaa agatgcacaa gatgctgtta
    10561 aaacttttga aaagaattca aatagtatga caaccgctgt tggtaaagtg atgcaaggta
    10621 ctggtgccgc aatgacaaag tatattacca ctcctcttat aggagtaggc gtagcagctg
    10681 ctaaagttgg tggcgacttc gaagcacaaa tgagtcgtgt aaaagctata tcaggagcaa
    10741 ccggcgacac attcgaacag atgaaacagc aagcgattga tctaggagca aaaactgctt
    10801 ttagcgcaaa agaatcagct gctggaatgg aaaacttagc ttctgctgga tttagcgcac
    10861 aagaaatcat gaaagcaatg ccgggtcttt tagacttagc agctgtatct ggaggggatg
    10921 tggctctagc ttctgaaaat actgctactg ctttgagagg atttggttta gaagcaagtg
    10981 aagcaggaca tgtcgctgat gtatttgctc gtgctgctgc ggataccaat gctgaagttg
    11041 gagacatggg agaagcattg aagtatgttg ctcctgtagc taattcaatg gggatttctt
    11101 tggaagaaac tgcagcagct attggtatta tgagtgacgc aggcattaag ggttctcaag
    11161 caggtacaac gttgcgagga gcattgtcta ggttagcaag gccaacaaag gctatgcaag
    11221 atacaatgga taatttaggt gtttcgtttt atgatgctga cggtaaaatg aaacctttaa
    11281 aaactcaagt agaattactt aaaaaagctt ttgaaggcct gacgcctgaa caacaacaaa
    11341 atgctttagt aacactatat gggcaagaat cattatcagg gatgatggct ttgattgata
    11401 aaggacctga ttcattgggc aaattaacaa aatctctgaa agattctgat ggtgcagctg
    11461 acgatatggc tcggaccatg caagataata tgaattcttc catcgagcaa atgtttggag
    11521 cttttgagtc agcagctatt gtaattcaaa agattctagc accatccatc aaaaaagtag
    11581 cagatgccat atccggctta gtagaaaaat ttgtaagtgc tccagaatca actcaaaaat
    11641 tagtagtggc cataggagca attgtcgctg ctataggacc gttaattttt atgattggtt
    11701 cagtaattat atggatcaat agggtgaaag tagcttttaa agctttaagt gaaagttcaa
    11761 aattgtttag tggattaagt aaagcaatgg gtcttcttac aaatccggtt tttctggtta
    11821 tagctgcggt agcactactc gttgtaggtt tcatctatct ttggaatacg agtgaagatt
    11881 ttagaaactt ttggattggc ttatgggagg gaatcaagtc tgctgtaagc tcggcagtag
    11941 aatggattca gaatgcatgg aaatctacag gagaatggtt taacaattta tggaagtcca
    12001 ttaaagaagg cgcagacaat gtttggacta cagttcaaga agcccctggg aaagcggcag
    12061 attggattaa aaacaaatgg actggaacaa aagagttctt ttcaaattta tggtcaagta
    12121 ttgcaaactc tgcttcagag atttggaata gtttaaaaga aggtgtcata tcagttattg
    12181 atgatttagt ttcaagtgct ggtgaaaaat gggaagggtt taaaaatact atatctactg
    12241 catggaaaac aattacaagt aaaatcaaat ctggttttga ttttatacta aaatatattg
    12301 gtccatttgt aagtagcttt tcagatgtgt tctctaatat agtgaaagca ataacaagta
    12361 tatttgctga ggttaaaaac ataatagtaa atgcttggga aatcattaag tctttaatag
    12421 ctgcgccact actgtttatt atagatttaa ttactggtga cttcgaacaa atgaaagagg
    12481 atttagatct aatctggaac acacttgtcc aatcagtggt aaatatttgg acatctgtaa
    12541 aaaatatatt tacggaatat atcggtgcaa tagtaaatag tgccgttagt ttatggactg
    12601 gattcataca aagtatttct aatatttgga atgaagtagt ttatcaagcg actatgattt
    12661 ggattgattt gaaactattt tttactaatt tatggattga tattaaatac agtgcaattc
    12721 aaatgtggat aaatctaaaa ttctccataa ttcaaacttg gattgataca aaatatggtg
    12781 caattgaact ttggaataat ctaaaacaat ggtttttcca aacggttaat aatatcgtgc
    12841 aaactcttat aaaaagttgg aacagcttaa agcaaggaac gatagattta ttcaacaata
    12901 cagttcaagg tgctaaagac atttggactt cattcaaatc ttggattggt gatttaatta
    12961 ctggaaccaa agataacatc attcagggat gggaaaacct aaaacaaggt actatagata
    13021 ctttcaacaa tttagtaaat ggtgctcaag aggcatggga taatttagta aatgctgtta
    13081 gtgacacggt tgatagagta actggctggt ttgataactt gaaaaatatc gatttactag
    13141 cagccggaaa agctatcatg gatagttttc tagaagggtt acaaaatgca tggaaatctg
    13201 tgcaagattt tgttggaggt attggtgatt ggattcgtga acacaaagga cctatccaat
    13261 acgatagaaa gctattgatt ccagctggtc aggctattat gaacggtctg aataaaggtc
    13321 tgacaggagg attcaatgac gtacagaata ctgttggaag tatggcagac tttatcgcgg
    13381 aacttttcaa tgcaaattct gatgtagata tagctgcaaa tttgaaaaat gcaaataaaa
    13441 acattggtgc acaagttgaa cataaaataa atatgggtgg ctctactaaa ccagctgtat
    13501 ttaaaatcaa tcttggaaga caatcgttta gattgtttat ggacgatatt tcacaagcta
    13561 tgggcgaagg tgcagatatt aatctggaat tttaggaggg aatattttgg atcaacgaga
    13621 aaataaaatg tactcattca aagatacaac cattaatctc actagttcta aacgattcct
    13681 tccgacgtct gccatgatgt acgatggaat gtatttagaa gatttgattg atggttatca
    13741 aacacttacg gtggaaggta gagaaatgct ttctgtagaa gttgaacagc aagagataca
    13801 aattggttca atcattacaa atcagaaaat accttcaaga acactaaaaa taacatataa
    13861 gctggaagat agagatccag aaaaactaca gtttaaattc aaagaactgt tgaattattt
    13921 ataccggaat gaagacgtgg aaattaggtt tcatgatgaa ttagattatt attactacgg
    13981 tcgctataca tcaactgata ctgttccagg agactccaac tcgattattt cgagttttaa
    14041 tgtattctgt gcggatccac taaagtatac gaaagagtgt gttagtgatg gctatattgg
    14101 aaatccgata cagtttccta taacaccaag aaaaattgaa gttactttat ccatgaataa
    14161 ttcaatcaaa attacaaacg gagaacaaaa tatcacgata actgacgcgg caataaaaac
    14221 aggagacgtg ttggtttttg atttttccga tgagcaggta actgtaaacg gagaagattg
    14281 tacttctatg attgatttag aaagtgattt tgagaacttt tatcttaagc aaggtcagaa
    14341 gataactagc aataatggga agcttaaaat attctatagg ggggcgacaa tttgagtgag
    14401 acagtttatt tctttgatca cttgcaaaaa cttattaaaa gaaaaaatac aagaagtttg
    14461 attgaagtct cccaagaaaa agaaattagt tctgataaga gtgatctaat gaaagatact
    14521 ctttacgtta cgacaaaata tgataaagaa atagaggatg caagatatat ggcgattcgt
    14581 gaaaacgagt cgtctttttc gttgtatcga attactaaag ttagcgaccc atttgaaaca
    14641 ttagagttta cagggttagg atttgcgaca aatgaattag atgcttacat catcaaagat
    14701 attaggccga gtgggcagcc cttaaaaaat gttcttgatc gattgattga atttactgaa
    14761 ggaaattggc gcgttggtca cgtagaagca atgttaccaa cagtaactgc aactttttac
    14821 tatgtctctg taaaagaagc gttgaaagaa ttgcaaacct taggtatgga atttgtcttt
    14881 aggtgttctt tgaattctga tggaataaag gataaatgga tcgaagtata tgaacaaatt
    14941 ggcgaagaat cgaatacacg tttcgtatat ggtagtaaag cattaacagt tgtaagagag
    15001 atagatagaa gctcaatctc aacttcaatg ataggtcgtg ggcgaggcga agaggttggt
    15061 gacggttacg gtagaagaat tgaattcact gatgttgaat ggaaaaagtc gaatggtgat
    15121 cctttagata agcctaaagg ccaaaattgg cttgaagatc cggaatcaac tcaaaagtat
    15181 gggataccac aaaaagatgg atcaatgaga aaacgagaaa ccgtagtagt gtttgatgat
    15241 atagatgatc caacagattt acttaaaaat acttattcaa ccttaatcga ttctgctaga
    15301 ccgttagtac aattcaaagc tgaagtcact ggaggagatg tgataggaaa tacagtgact
    15361 attcacagat acgataaagg ttatcactat aaaactcgta tttataaaac tacattcaat
    15421 cggcttaccg gtcaaacgaa tatcgaacta ggggataatt taacacaaga tgttagaaaa
    15481 caaacggctt ctattgtcaa taatattaat agtttagaat ctagcaaaat gacattttac
    15541 gaatcgacag agattggaaa atatcaagat gacattatgc gaggtgcagg agataatggc
    15601 ggttctattt attgggtaaa tggaattgaa gctggtgtta gtgatagtag agaaatctat
    15661 gaaactgttt atatggatgg acctaacatt cctagatcac gcttttttat ggtccaaaat
    15721 aactcaggga tatctttcaa acaatgtaaa aaaggtgagt ggcaaacaat tcaagatgta
    15781 cacaatggtg atagcacgac cgcttggacg ttggatggaa ctttcaatgc taattttatt
    15841 cgcgcaggca ttctgtctgg tatcctcgtg caaggggtag ctttaaaaac atttgacgat
    15901 aaagatttcc aattagtggc agagggggga gaactttctt ttgaaaaaca agtcaagtct
    15961 actggactag atgatgtaca tggagaacgc ttgggctcaa ttgtatctac ttatggttct
    16021 aaagggatta acggctttgc tgtctggaaa gagccaaact atattttttc cattaatgct
    16081 ggggacggtg gcgatcgagg gaatcctatt tttcaaattc cagcagatgt tactgctgat
    16141 aagcgcaaat ataatcttta tggtgacggc aatttttcag aaggaaatat aaccatagat
    16201 ggacggctag atgtaaaaga actctatgtt aacggtacaa aaattgacac taatggagga
    16261 ggcaattccg gagggaacga taacggttgg aacggacaat acccaccaga agtaactact
    16321 gatcgggata aacgatattg gcaaatttgg gcaacggcaa taggtgccgg ttttacacaa
    16381 caagctgctg cagccttact tggaaatgca caaggagaat cagatgctaa tccaaccgct
    16441 gatgagggca atggcgcacc agggttcggt tatggtgtat ggcaatggac cgattcttct
    16501 ggcgcaacta gcggacgtgt ctacatgatc aatttaatga caaaggctgg catcagtgat
    16561 gatccagaca caatcacggc acagtttaaa ttgttgatgt ggcatgcacc aaatggtcaa
    16621 tggatcgcaa ctagcgctta tccttacaca tggacacaat tcatgaatct gaccgatatc
    16681 aacacagcag cacaagcatt cgtggctaac tttgaacgtc cacgtgatcc acatccagaa
    16741 cggacggcat ggtcacaaga atggtatgac aagttcaaag atttggaaat tcctgtatca
    16801 aaaggatata tgaagccgat tgcggatcca atcacagtga cgagcgaatt tggctggcgc
    16861 acttctccaa tcacaggagc acaagaattt cataacggta ttgaccttgt aaatggaaat
    16921 cctaatacac ctatttttgc ttcagcggat ggcgaagtga ttgttgcagg tgatgcaaat
    16981 tactttgact ggtatggaaa ttggacagtg attaaacacg ctgatggaat gtatacagga
    17041 tacgcgcatc aaagccgagt agacgtctca aaaggtcaga aggtaactgc tggtcagcaa
    17101 attggactga tggggacaac aggaccatcc actggagaac atcttcattt tcaatttatg
    17161 gatgagtttt atccatcttc ttcaggtcat ttccacaatg caagagacta catcgatttc
    17221 taaaggaggg atagtcatgg cagaaacgca gcataaaatg gtcctatcca ccaccgaacc
    17281 aaacaacgga ataaatttgg ttcgaattcg gcaaggggat gttttaacgc aaaagttcgt
    17341 tgttgaagtg gtggaacatg gcaaactaaa aacattcgag ggcctagtgc cgttttttat
    17401 taatacaaca aaatttggcg aaaaccaacc tgttgaacaa aaagtacaag aatacagtcc
    17461 agcgcaagca aggcttgttt acaccttaag tgagcctgac tggcaatggg gtggtgaaaa
    17521 caccgcacat ttcagtttcc gatcacttaa tggtgatgga acttggagtg aacaatttag
    17581 cacacaggat tttacctatc gagtcatttc tggaatatct agaagccagt tacgtgactc
    17641 tggctatgtg tggacatttg aggatttgct aagaaaattc aaagattaca tggatcaggg
    17701 caaaaatgac tgggagcagt ggttagaaga taatcgtgaa atactggaaa atatcgatcc
    17761 aggtggtatg atcattaaca ttctgaatga agctaaaggc gactacgaat cattagctga
    17821 tcgtttaaac caaaaatacc aagtgccagt cggcagctca caaattagag aaacaacacg
    17881 cttttttgat tacgacacga tgaaatacgt tgacctagtg ccgcgcaatt tgaatacggt
    17941 tgtcaacagt gttaataaca gcaaatttaa cttttctttc attacagaca ttcacgcaga
    18001 taatcacaac ttgcgtatag atggcgtcgg ttacaaagat gcttcttatt taagacattg
    18061 gcgcgcaatc cctcaatttc aaaaattagg gaacaaaaca gatgtgatga tttacggcgg
    18121 agacaatatc gacggcggac ttggttcact cggcagcgat ataggcatta ttgatgaatg
    18181 gagcgcgcga cattccatgt taggcacgct caaacgcttc actaacgcag cggtagcagg
    18241 acaagaaaaa ccggttatta tctgcaaagg gaatcacgac gcttgtttcg accccgcgtg
    18301 gcgcaagcga aagggaatgt tatgcaacgc tgacttcgaa gagtattgga acggtttgta
    18361 cggtggcgtg ttgttcccag ataaaaacgc agcaatttac cgtttcgata cttgtgattt
    18421 ttatgaaggc ggcacaggcg acaagtacgt ggacggttac agcgatacag cgccgggagc
    18481 tttcagtgcc aaacaaatta acgctttcgg agaatggtta gtgaacgttc cgagaaacta
    18541 tcatgtggcg ttagtaggac atacgccttt aggtctcagc aagtttcccg ttcgcaatga
    18601 aaacatgatt agcacgttga tagaagggtt taaatctggt agtccggtaa ctatcgattg
    18661 gtctaaatta ggacaaccga atgacggttc gttcggcgga ttaaagacct tcgcaatgaa
    18721 tacaaaaggc gccggcgttg tagtaggtta tttctgcggg cattggcacg aacaagtcga
    18781 aggggcgttt ggaacagtaa aaatgattct ttgcgacgtc ggcttctctc atacagctag
    18841 ccaagttgat acaccggatg aattagcgtt ttataaaata gaagttgaca cagcaacaag
    18901 aaaagtgaca agcaaaggcg tagggcgcgc gagagacttc acttataact attaaggcgg
    18961 tgaacaatta aatgttagat tttcaaagca aaccaaatat ttttgaagaa atgagttatg
    19021 aagaggctgt gaaatggtta ttgcgtcaag cggctatcca ttacgacggc tcggatcacg
    19081 acgcgcacgt cctagctact gaaagcaacg cgggctttgc tacaccggaa acagttatgc
    19141 aagcgcgtgg gcgttggtta cgcgattata agctaccaca aaaatatccg aacattttag
    19201 acattccgcc cggcaaatac gcaaccaaag ccggatgggg cgcagacaac cctgacggga
    19261 ttgaagatga cagttttgtt gaaatgatgg tattcgcgga tcacgattcg agaaaattaa
    19321 tcgtcgcttt tgctcgttat agcggcgaaa tttacatcaa aatgacacat aacagcgaac
    19381 cggtagaggg ttataactcg ttaggctggc ggcgcgttta cactacttct gttctttttg
    19441 aaggcgaatt aagaaaagga caatcagtca atctgccaga tgatactttc cgttaccaaa
    19501 cgcttcgtat ccactacaca gacggtgacg gagactttgt agaagaagtg aaacgtcaaa
    19561 gatacgcgcg aattacaaaa gcgaatttat ggaatggaga cgcgggagtg acgttcatcg
    19621 aatttgagct gacaatcgaa gcgcgaaaga taacgatgtc aaacggcaga gctttagata
    19681 tttcatccgg caatgtttcc aatcccgcaa tgagcaacaa cgtgaaaata actaagattg
    19741 aaggtgtgaa ataatggcgc atgtcatcaa aaaaggctct atcaaagtac ctacacagcc
    19801 gaaagactac gatttgcaag caacggggct tgtatttaaa tcatacgata atcaaatagc
    19861 gttagagttc aacgtcgaac aacagaacgg cacaccggcg gacttgctag gagctaactt
    19921 gcgcttgttg atgtttatct atgatgaagt agatggaatg atcacgaaag agccaatccc
    19981 ttttatcacg aaaaacctca tcactgaaag cttcttgaat ggacaggtcg tatatatctt
    20041 gccagaagcg atgaaagctt ataacggtat ggtggaagct tatgtttaca tcgagtatcc
    20101 agacggatca acaagtgata acttaggctt caccttccgt atgaagcgtt cagcaatcga
    20161 cggactagcg caagataaag cagactactt tattgcagac tttcaacaat tacttgatgg
    20221 agtcaaacaa gaagcaacag atgctgttaa cgcagcatta acaaatgtgg attctacgat
    20281 tgaaaaggca aaccaacaaa taaatgaatt tgtagaggga gccacacaag caattgatca
    20341 aactgttgac gaggtaacag agcaactaca agctactcaa actaagattg ataccgtttc
    20401 tcaaaacgtt acatcggcac aaaacaatct taaagcagtt gaagacaaga tgaatcaaac
    20461 caatcagcaa atcggcgacc tcggcaagct gaaaaagatg tacagtaaca gcatcgactt
    20521 cgggaattat gattatagtg ggaatccgaa tttaatgtcc aaactaaaat cgagcgattt
    20581 taacgttggt taccacgggt cactaacttc ggataacgaa aagctacatt ttacttctga
    20641 tggtacagga agcattatta tgtttacgcg tattaataca cctcagcttg ctagtgggaa
    20701 aacctatact ctgagtgcga aagttcgatt tgatgaagga actacaggag ctattgataa
    20761 attacgtttg gtgtatcgta catcaccagg agaaaagata ttattggaag caaatagtac
    20821 aaatattaca acagatgatg tagggaaaga aataacaatc aaaggtacag ctaacgttaa
    20881 ttatcaaatc acaaatttag atcgatttta tatgagtatt agctttgttg acagggataa
    20941 aataaatggc ggatttaagt tgtacgacat caaaat
//
LOCUS       DANKHJ010000044        20886 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000044 DANKHJ010000000
VERSION     DANKHJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            112..306
                     /locus_tag="PW113_001791"
     CDS             112..306
                     /locus_tag="PW113_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526105.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="PW113_001792"
     CDS             575..874
                     /locus_tag="PW113_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526106.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="PW113_001793"
     CDS             complement(971..1405)
                     /locus_tag="PW113_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526107.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="PW113_001794"
     CDS             complement(1637..2347)
                     /locus_tag="PW113_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2526108.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="PW113_001795"
     CDS             complement(2337..3197)
                     /locus_tag="PW113_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2526109.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="PW113_001796"
     CDS             complement(3202..3840)
                     /locus_tag="PW113_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2526110.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="PW113_001797"
     CDS             complement(3855..4154)
                     /locus_tag="PW113_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526111.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="PW113_001798"
     CDS             complement(4197..5681)
                     /locus_tag="PW113_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2526112.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="PW113_001799"
     CDS             complement(5701..6162)
                     /locus_tag="PW113_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2526113.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="PW113_001800"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="PW113_001800"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2526114.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="PW113_001801"
     CDS             7465..8235
                     /locus_tag="PW113_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2526115.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="PW113_001802"
     CDS             complement(8508..9335)
                     /locus_tag="PW113_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526116.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="PW113_001803"
     CDS             9903..11138
                     /locus_tag="PW113_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2526117.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="PW113_001804"
     CDS             complement(12137..12397)
                     /locus_tag="PW113_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2526118.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="PW113_001805"
     CDS             complement(12424..13725)
                     /locus_tag="PW113_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526119.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14017..14475)
                     /locus_tag="PW113_001806"
     CDS             complement(14017..14475)
                     /locus_tag="PW113_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2526120.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14488..15819)
                     /locus_tag="PW113_001807"
     CDS             complement(14488..15819)
                     /locus_tag="PW113_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL2526121.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15830..17272)
                     /locus_tag="PW113_001808"
     CDS             complement(15830..17272)
                     /locus_tag="PW113_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2526122.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17259..18023)
                     /locus_tag="PW113_001809"
     CDS             complement(17259..18023)
                     /locus_tag="PW113_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2526123.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18127..19002)
                     /locus_tag="PW113_001810"
     CDS             complement(18127..19002)
                     /locus_tag="PW113_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2526124.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19257..19341)
                     /locus_tag="PW113_001811"
     tRNA            complement(19257..19341)
                     /locus_tag="PW113_001811"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19305..19307),aa:Leu,seq:caa)
     gene            complement(19359..19430)
                     /locus_tag="PW113_001812"
     tRNA            complement(19359..19430)
                     /locus_tag="PW113_001812"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19396..19398),aa:Gln,seq:ttg)
     gene            complement(19440..19512)
                     /locus_tag="PW113_001813"
     tRNA            complement(19440..19512)
                     /locus_tag="PW113_001813"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19477..19479),aa:His,seq:gtg)
     gene            complement(19574..19658)
                     /locus_tag="PW113_001814"
     tRNA            complement(19574..19658)
                     /locus_tag="PW113_001814"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19622..19624),aa:Leu,seq:caa)
     gene            complement(19679..19749)
                     /locus_tag="PW113_001815"
     tRNA            complement(19679..19749)
                     /locus_tag="PW113_001815"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19715..19717),aa:Cys,seq:gca)
     gene            complement(19795..19866)
                     /locus_tag="PW113_001816"
     tRNA            complement(19795..19866)
                     /locus_tag="PW113_001816"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19832..19834),aa:Gln,seq:ttg)
     gene            complement(19878..19950)
                     /locus_tag="PW113_001817"
     tRNA            complement(19878..19950)
                     /locus_tag="PW113_001817"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19915..19917),aa:His,seq:gtg)
     gene            complement(19953..20026)
                     /locus_tag="PW113_001818"
     tRNA            complement(19953..20026)
                     /locus_tag="PW113_001818"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19992..19994),aa:Trp,seq:cca)
     gene            complement(20033..20113)
                     /locus_tag="PW113_001819"
     tRNA            complement(20033..20113)
                     /locus_tag="PW113_001819"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20077..20079),aa:Tyr,seq:gta)
     gene            complement(20115..20190)
                     /locus_tag="PW113_001820"
     tRNA            complement(20115..20190)
                     /locus_tag="PW113_001820"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20155..20157),aa:Phe,seq:gaa)
     gene            complement(20226..20298)
                     /locus_tag="PW113_001821"
     tRNA            complement(20226..20298)
                     /locus_tag="PW113_001821"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20263..20265),aa:Asp,seq:gtc)
     gene            complement(20308..20380)
                     /locus_tag="PW113_001822"
     tRNA            complement(20308..20380)
                     /locus_tag="PW113_001822"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20345..20347),aa:Val,seq:tac)
     gene            complement(20396..20467)
                     /locus_tag="PW113_001823"
     tRNA            complement(20396..20467)
                     /locus_tag="PW113_001823"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20432..20434),aa:Glu,seq:ttc)
     gene            complement(20482..20571)
                     /locus_tag="PW113_001824"
     tRNA            complement(20482..20571)
                     /locus_tag="PW113_001824"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20535..20537),aa:Ser,seq:gga)
     gene            complement(20611..20684)
                     /locus_tag="PW113_001825"
     tRNA            complement(20611..20684)
                     /locus_tag="PW113_001825"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20648..20650),aa:Asn,seq:gtt)
     gene            complement(20694..20809)
                     /gene="rrf"
                     /locus_tag="PW113_001826"
     rRNA            complement(20694..20809)
                     /gene="rrf"
                     /locus_tag="PW113_001826"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6580 a         4385 c         3325 g         6596 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
    13801 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
    13861 ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc
    13921 aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc
    13981 ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt
    14041 aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac
    14101 aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag
    14161 tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag
    14221 ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt
    14281 tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg catggatata
    14341 tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc
    14401 ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt
    14461 tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac
    14521 tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa
    14581 tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt
    14641 ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag
    14701 tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga
    14761 ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc
    14821 attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt
    14881 caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc
    14941 tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat
    15001 atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt
    15061 gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc
    15121 gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa
    15181 ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat
    15241 ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat
    15301 atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat
    15361 cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc
    15421 gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca
    15481 cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc
    15541 caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg
    15601 atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac
    15661 aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa
    15721 tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc
    15781 gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta
    15841 ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat
    15901 ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga
    15961 ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga
    16021 tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac
    16081 ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt
    16141 cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca
    16201 aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat
    16261 tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt
    16321 aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa
    16381 gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac
    16441 cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct ttatagatag
    16501 ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc
    16561 cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag
    16621 gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa
    16681 aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa
    16741 cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa
    16801 aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa
    16861 aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta
    16921 ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta
    16981 agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct
    17041 tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa
    17101 cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca
    17161 tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct
    17221 gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact
    17281 aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg
    17341 acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac
    17401 gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc
    17461 aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca
    17521 tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc
    17581 cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa
    17641 gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt
    17701 tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga
    17761 taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt
    17821 ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt
    17881 agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt
    17941 cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc
    18001 gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa
    18061 ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc
    18121 cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat
    18181 ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt
    18241 gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata
    18301 aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc
    18361 tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc
    18421 tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat
    18481 agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc
    18541 gaccacttct actgctatat catattcatt tggataaagg aatctcattt ctggcaacat
    18601 aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt
    18661 caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat
    18721 cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg
    18781 tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg
    18841 cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc
    18901 gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc
    18961 tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa
    19021 acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt
    19081 gaatagtagc accctacata atttacaaaa atagtttaac acataataaa agcgttatca
    19141 atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca
    19201 taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga taattatacc
    19261 ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt ccagcgcgtc
    19321 tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg gattcgaacc
    19381 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaagg
    19441 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
    19501 cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac taagatgaag
    19561 acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg ggcactggat
    19621 tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat tagtactaag
    19681 gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt accacttggc
    19741 tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata gaaactgggg
    19801 tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat
    19861 accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
    19921 ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac
    19981 actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag
    20041 agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact
    20101 tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat
    20161 ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag
    20221 aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
    20281 cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct
    20341 gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc
    20401 cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
    20461 acgggccagg taaatttgct acggagagta agggattcga acccttgaga cagtttggca
    20521 ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa
    20581 gaagctcaat actctttctc aaaatgagaa ctccggcagt aggactcgaa cctacgacat
    20641 catgattaac agtcatgcgc tactaccaac tgagctatgc cggaataaca tcagcgtggc
    20701 gacgtcctac tctcacaagg ggcaacccct cactacaatc ggcgctaaga agcttaactt
    20761 ctgtgttcgg catggttaca ggtgtatcct tctcgctatc gccaccacac tgtggtgtta
    20821 tcttttattg agtaaatttt gttcactcaa aactggattt gaagtcacat aagttttttt
    20881 ccgagt
//
LOCUS       DANKHJ010000045        20463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000045 DANKHJ010000000
VERSION     DANKHJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..405
                     /locus_tag="PW113_001827"
     CDS             <1..405
                     /locus_tag="PW113_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2526125.1"
                     /translation="TLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFF
                     RGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTK
                     VTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            453..2582
                     /locus_tag="PW113_001828"
     CDS             453..2582
                     /locus_tag="PW113_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2526126.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            2557..3246
                     /locus_tag="PW113_001829"
     CDS             2557..3246
                     /locus_tag="PW113_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526127.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(3654..4331)
                     /locus_tag="PW113_001830"
     CDS             complement(3654..4331)
                     /locus_tag="PW113_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526128.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(4467..4661)
                     /locus_tag="PW113_001831"
     CDS             complement(4467..4661)
                     /locus_tag="PW113_001831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526129.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(4711..5151)
                     /locus_tag="PW113_001832"
     CDS             complement(4711..5151)
                     /locus_tag="PW113_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2526130.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(5155..6000)
                     /locus_tag="PW113_001833"
     CDS             complement(5155..6000)
                     /locus_tag="PW113_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2526131.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6149..7567
                     /gene="pepV"
                     /locus_tag="PW113_001834"
     CDS             6149..7567
                     /gene="pepV"
                     /locus_tag="PW113_001834"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2526132.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(7624..8571)
                     /locus_tag="PW113_001835"
     CDS             complement(7624..8571)
                     /locus_tag="PW113_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2526133.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8794..9144)
                     /locus_tag="PW113_001836"
     CDS             complement(8794..9144)
                     /locus_tag="PW113_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2526134.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            9344..10423
                     /gene="pepA"
                     /locus_tag="PW113_001837"
     CDS             9344..10423
                     /gene="pepA"
                     /locus_tag="PW113_001837"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2526135.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            10567..10887
                     /locus_tag="PW113_001838"
     CDS             10567..10887
                     /locus_tag="PW113_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2526136.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            10909..11373
                     /locus_tag="PW113_001839"
     CDS             10909..11373
                     /locus_tag="PW113_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2526137.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            11578..12183
                     /locus_tag="PW113_001840"
     CDS             11578..12183
                     /locus_tag="PW113_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2526138.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(12222..13511)
                     /locus_tag="PW113_001841"
     CDS             complement(12222..13511)
                     /locus_tag="PW113_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2526139.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            13939..14418
                     /gene="rlmH"
                     /locus_tag="PW113_001842"
     CDS             13939..14418
                     /gene="rlmH"
                     /locus_tag="PW113_001842"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2526140.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            14503..15012
                     /locus_tag="PW113_001843"
     CDS             14503..15012
                     /locus_tag="PW113_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL2526141.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            15112..15762
                     /locus_tag="PW113_001844"
     CDS             15112..15762
                     /locus_tag="PW113_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526142.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            16213..17151
                     /locus_tag="PW113_001845"
     CDS             16213..17151
                     /locus_tag="PW113_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2526143.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            17164..18216
                     /locus_tag="PW113_001846"
     CDS             17164..18216
                     /locus_tag="PW113_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526144.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            18482..19117
                     /locus_tag="PW113_001847"
     CDS             18482..19117
                     /locus_tag="PW113_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2526145.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            19308..20099
                     /gene="msrA"
                     /locus_tag="PW113_001848"
     CDS             19308..20099
                     /gene="msrA"
                     /locus_tag="PW113_001848"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2526146.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         6511 a         3601 c         4197 g         6154 t
ORIGIN      
        1 accttagcga tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt
       61 ggagattgga taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca
      121 ctaggatttt tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc
      181 gtattagctg ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca
      241 acgattgctc ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat
      301 acaaaagtaa cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga
      361 gcgatcttat gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga
      421 taaaaacgat tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg
      481 atgttggcgg tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg
      541 gaaaatcttc agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc
      601 agtcttttca aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg
      661 cacattctac cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg
      721 taatcgggat ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga
      781 acgatatcga tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca
      841 aacacttaaa tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg
      901 aggtcttggt ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt
      961 atgaagcagc atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat
     1021 atggattgac aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc
     1081 tagatacggc aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga
     1141 tgattatgcg cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt
     1201 taaccatgct ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt
     1261 ctaatggtgt ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg
     1321 gacgtccagc aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag
     1381 atgttcgggt tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca
     1441 tcgatgtcgg tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga
     1501 cagaaaaaat caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg
     1561 attacgtcaa ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag
     1621 ccaatgtcct tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac
     1681 gaaaagccat ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac
     1741 agatcatgga aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt
     1801 atcacttgga gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga
     1861 ttacttactt tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga
     1921 tttcttctat tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt
     1981 cgccaagtaa agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat
     2041 caggcgctac accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag
     2101 taacggctat cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca
     2161 caacagaaga agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt
     2221 gcttattgga aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg
     2281 ctggaagtct tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt
     2341 catgtatgaa aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata
     2401 tggcagttta tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa
     2461 gagtctctga ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag
     2521 tatctactat ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat
     2581 aagttagaag agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac
     2641 cagtttcggc gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata
     2701 caacaatcaa tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt
     2761 gccgatgtag gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag
     2821 caaataaacg aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg
     2881 acggagccgt tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg
     2941 caagctttga ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt
     3001 tatacgcaaa gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac
     3061 attcgtgcat tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat
     3121 ttttcgcctt atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa
     3181 gggatcttag aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac
     3241 aaataaagag actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacgatgtt
     3301 ctcctgtcaa ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt
     3361 gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg
     3421 ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt
     3481 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg
     3541 gtgtgcaatc agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta
     3601 ttttgccgtt tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg
     3661 atgggtctgt cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc
     3721 tcctcgataa tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga
     3781 tcaatcattg attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct
     3841 gaaatcgtca aacgtaacca tctgaaacgc gtactataac tttctagcca atcccaaatt
     3901 tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa
     3961 gcttttttat tttgagcgat tactgcctga tcggtttgtt ctagtatttt ttctagttta
     4021 gcttgatctt gcttggtcat tttcgcactg cattcttgca ggatcggttt ttccaacttt
     4081 tcccgcgtat aacgggctaa tgaagcagat cggatatcga tttttgaaac ataagtgccg
     4141 gactgtggaa tggtttttac aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc
     4201 ggtgtacgcc cgatagctag ttgttcttct aacactttat cggagatttt acttccaggt
     4261 gctaactcgg aataaatgat ttgtttacgt atagattcat aagcttgctg ttgcaaatta
     4321 ttgataacca tttaaccttc ctttctatgc agatataatc tattataaca taaatcgctt
     4381 acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag ccctgatagg catgcctatt
     4441 agagcctcga aaagaaaaat attgaatcaa tctggtcgta cctcttggat cggtttgaca
     4501 cgaccgccga ttcttttagc aaaagcttcg gattcttttt tgtctttgaa aatgagcaga
     4561 ttgtctaagt ttttttcccg agtgcgacag ccagttttat cgatgtaacc gtgcattact
     4621 ttaacgacat acatggggat cacctgcttt tttcttttca ttatacgaac tactcgacac
     4681 aatacaagaa aaatgcttag acggtcttta ttaagaattt acatcatcca aagaagcgta
     4741 cgcttggaat aactgatcta actccggatt gactttgctc agcttcacta atcgttgcga
     4801 atcagaaagt aaaaaacagt gtttactact gccagtagtt tttaattttt tgtcttcagt
     4861 tcccacaatt tcgtagctaa aatccaaccg tgtccccgta tacttttgga tagttggatg
     4921 gatgctcact ttttctccgt aatggatcat ttctttatag ctgcaattta cttccagaac
     4981 ggggatgatg atcccagatt cttcgatttt tttataggga aaccctagat gtaccaataa
     5041 ggctacccga gcctcttcaa accagcggat ataattgctg tgatgaataa tccccatttg
     5101 atctgtttca taataaaaag gttctcgcac ataacctgta aatttctcca tgactcatgc
     5161 ctcgttttcc attgattttt tacaaaaata attcatccat ttcggaagtg cttcgctgat
     5221 ttgtgtcggc aaaacaatat attgttcttt tgccagctgg tctccaatga aactatgaag
     5281 ataaatagct gctgcaatcg tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat
     5341 catcccagct aaagtgtcac ccatgccgcc cgttgccata gcagcagaac ctaaaggatt
     5401 ggtgtagaag tgctggtgac cataaatttt tgtctcatga cttttcaaaa cgagcacagc
     5461 accgatttcc tgttgcttct ttttgttttc tttatcccgt tgttcttgta tcggtagaga
     5521 actcaaccgt tgccactcca tttgatgagg tgtaagtaca gtttgttcgg gatagggtag
     5581 caaaagatga ttagcagcaa gtaaacttaa agcagagcca tcaatcacca gccattgttc
     5641 ttttgattgg ttttctagta cttgtttgaa acgttccaaa ctcttagaag aagtacccaa
     5701 tccgggacca atcaagagta catctgcttg ctttattacg gaaagaaact ggtcagtatc
     5761 cgtccaaact acaatcattg cttctggcag atgactgtga agagcactcc gatttttttc
     5821 atcagttact acagtcgtga gacccgctcc gctgtgtacg gcagctaaag cactcataat
     5881 gattgctccg ccatattgtt cattgccgcc aatcaaaacg atacgtccaa atgttccttt
     5941 atgtgaaatt tttggacgag gtgtgatgac tcttttcact agttgttcgt ttatcagcat
     6001 ctaaaaacct ccttttactc attatagcgt atattttttt tagagaaaaa cagataagaa
     6061 aaggaattgt cagatttttt gaactatttt tttctgtaat ttgtggtatc atgaacacga
     6121 agacttaaat aagtggagga ttaactttat gacaattgat tggcaaaaag aagtagaagc
     6181 acgtaaagaa gacttgcttg aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga
     6241 tgactcaaaa gtaacaccag atgcaccatt tggacctgga cctcgcgacg cattgaaaca
     6301 tatgttagct tatggcgaac gtgatggatt taaagtaaaa aatgtcgata actatgcagg
     6361 gcatattgat cttggcgaag gagacgaaac gctagggata tttggacaca tggatgtcgt
     6421 tcctgcagga gatggctggg acacagatcc ttatgaacca gtaatcaagg atggaaagat
     6481 ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg gcagcttact atgcgatgaa
     6541 gatcattaaa gaacttgact tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga
     6601 tgaagaaagc ggctggggcg atatggctta ttatttcgaa catgaagaag aaccagattt
     6661 tggtttctca ccagatgctg aattcccaat catcaatggt gaaaaaggaa atgtttcttt
     6721 agctttacgc ttcaaaggcg acaatgcagg agattatgtc ttgaaatctt ttgtttccgg
     6781 acttcgcgaa aatatggtgc caggaacagc aactgcagca cttcaagtac caagtgcaga
     6841 tgcagctatc gcgatggaag aagcatttta ccaattcatc gaagcaaatc cagtaagcgg
     6901 aacgattgaa gcggacaata cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg
     6961 tgcaagccca caatctggta tcaatgcagg ttctttcttg gcattgttct tagataacta
     7021 tgaattccta ggatctgcta aacaatttat ccatgtagca gctgcttatg tccatgaaga
     7081 tttttatgga gaaaaactag gagtcgctta tgaagatgaa aaaatgggca aattgacaat
     7141 gaatgctgga ctatttgctt ttgaagaaaa tggtacagag gaagcgaact tcatcaatat
     7201 gaatttccgt tttccaaaag gtgtaacagt agacggattg caaagtgaca tcgaacaaac
     7261 agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc gtaatggaac cacattacgt
     7321 accaatggat gatccattgg tggctacatt attgcaagtc tatgaagacc acactggaga
     7381 aaaaggctac gaacaaatca tcggtggcgg aacttacgga cgtttgttga aacgtggcgt
     7441 tgcttatgga gcgatgttcc caggatatac tgatacaatg catcaagcaa acgaatttat
     7501 gagtcttgat gacttgttca gagcaacagc aatctatgca gatgccatct atcgtttggc
     7561 gaaataatta attaaaaaga ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt
     7621 tttttacttt ctactgtggt attcatgata aacatgatat agattcaatt catattcttt
     7681 cttagcgttc gtgacgactg tatccaaaat caaccctgtt actaatgaaa gaagtgctaa
     7741 agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat
     7801 atattcgcga atcacaggaa tccccgtgac caatccaaat aaaaagaaga agatggtcca
     7861 aatcccaaag aaaagcaaag gcttataatc tttaaacatt ttaataatca tcatgatcac
     7921 tttgaatccg tcagagaatg tattcaattt tgattcgctg ccttcaggac gatcacgata
     7981 atcgattggg atttcaacga acttgaattt tttatctaaa gcatggatcg tcaattccgt
     8041 ttcgatttga aaaccagaac tcataatagg aaaacttttg acaaagatac gattgaatcc
     8101 tcgatagccg gtcatgacat caagaatttt tgttttatac attctagtaa ttgtatttct
     8161 aacaaggtta tttccaaaat cgtggaaagg tctcttattc tcatcaaagt aggttccatt
     8221 tgataggcga tcaccaatga ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc
     8281 atgtacagct tctgcaggat aagtatcatc gccatctacc attaaataat agtcggcatc
     8341 gatatcaaag aacatttggc ggatcacatt tccttttcct tgacgaggtt ctttttttac
     8401 gattgctcca ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac
     8461 ataaatatct gcttctggta attctcgttt aaagtctgca atgactgttg aaattgttgc
     8521 ttcctcattg taacaaggaa tcagaacagc aattttttct tgttgttcca tttttcactc
     8581 tccttaacta tagaaaacaa aagctaaatt aaaaatacct cataatcaaa ggaaatgcta
     8641 gtttttcccg ctaaatatca tttatttaaa aagaaaatac tggaatatat gtttggaaag
     8701 ctctatctaa aaaaccaccc actacaaaag aaggacatga tacctctctt ttgaatagtg
     8761 ggtgcgtatt cgttctctca cttttttgac gtattaatct gttgcacctt tttttcttga
     8821 aatttctaat acggttcctg tatgagcatc tgctaaaaat tcataataaa cagtttcgcc
     8881 atcttctatt cgagaaagac ctccccgata agcactcgac cgaacggcga atttcctaat
     8941 aggttccttt tcaaaagaga tccaagaacc ttcgattgca ccttctttta gaaaagatga
     9001 cttgatattt tcaagtactt ggtctgctga caatactttt ttgcgatgat accaaatcgt
     9061 tgaagctacc ccgctcaata atcctagacc gattccaact gctaacccgc ctttaaaata
     9121 gcttagttcg ctttcttcat acatgtggac atacctcctt cttctcactt gttactcctt
     9181 gtgcttattt taccacactc tcatattttc tttgaaataa ataaccgcta ccagaaaaaa
     9241 taagctataa taaatacgat cggtctgcag aaaaaatcat attttatccg cagatcaata
     9301 taagtctgag aggagtttag tctgaaattt agacgaatag catatggaag aaaaaacatt
     9361 tcaacgaatc aaagagttaa cagaattaca aggaacaagc ggttttgaac aagatgttcg
     9421 cgcttatatg gaaagtcata tggaaccgct ggtagatgag ttacagttag atggtttggg
     9481 cggcattttt ggtctgcgcc atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc
     9541 acatatggat gaagtcggat tcatgctgac acaaatccaa gaaaacgggc tttttaaagt
     9601 cgtacctcta ggcggctgga atccttacgt tgtttctgct cagcgtttta cacttaaaac
     9661 ctctactggt aagaattatc catgtatttc ttcttccgtt cccccacatt tattgcgagg
     9721 aacaagcgga caaaaatcag tagaagtaac cgatattttg ttcgatgctg ggtttgaatc
     9781 aagagaagag gcaatgagtt acggtgtcct tccaggagac acaatcgttc cttacgctga
     9841 aacgatcaaa acagccaacg gaaagaatat tatcagtaaa tcatgggaca accgctatgg
     9901 ctgtacaatg gtattagaag ctttagaagc gttgcaaaat gaaacattag gtcatacatt
     9961 gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa
    10021 taaattcaag ccagatctgt tctttgcagt agactgctct gcagcagatg atacagtgac
    10081 gaaaaatggc acattcggac atttaggaga aggtacattg atgcgtatcc aagatccagg
    10141 tttgatcatg cttcctcgtt tgcgcgagta cttgctggat atcgctgaaa caaacaatat
    10201 cccttatcaa tactttgttt caaaaggcgg tacagatgca ggtgctgctc atacacaaaa
    10261 cgaaggaatc ccaagtacag tgattggcgt agtgggacgt tatatccata cgcatcaaac
    10321 gatgttcagt attcgcgatt ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg
    10381 tttggataaa tcaacagtaa atacgatcgt atacggaaaa taatgcgata gtgaaactat
    10441 aaaatctggc aaaagattgc tttttgccag atttttttta taaaaagagg atcaatgttt
    10501 gttagttcga aagaaaggtg tataatttaa ttgaaaaata cataaacaga aagtagagga
    10561 caaagaatga ttatcccaaa tagtttagaa gaattagcag gctacgtaga aactggaaag
    10621 agtgtatttt tctttactgc cggctggtgc ggagactgcc gttttatcaa accgcaaatg
    10681 cctgaaattg aaaatgactt ttctgattgg caatttattg aagtagaccg tgacaagtac
    10741 atagatgtag ccgctgaatg gaacattttt ggtatcccaa gttttgtcgt gatccaagac
    10801 ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg
    10861 ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag gggtcaagat gaatcaagaa
    10921 tataagaata ttttagtagg tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa
    10981 gcggtagaag tggctcgccg taataatggg aaagtatatg tagcaaacgt aatcgatcag
    11041 caattttata actttatggg ctatgcacct attgatcaaa atctagtcga tcagcaaact
    11101 gaaacagcca aggaaatgat cgaagaatgt aaagaatatg ggaaatctgt tgattacaca
    11161 aatatagaag gaattattgc atatggttct gttcgtgaat ccatggcagt caaattgcca
    11221 aaaaaatatg agatcgatct aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc
    11281 tttatgacag gaagtgtagc cagctacgtg atacgtcatt caccttgtga tgtcttgatc
    11341 gtttctgacg aagaagaaca gccaagtaat taagcaagtg gaacttgtac gaccttttta
    11401 atcaattaac taaaacagta aagggactgt gacaaaagtc ttgtcacagt cccttgttac
    11461 gaataaatgg tgttccagaa gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc
    11521 agtgaatgca attttttgtt aagataaatc tcagcaaagg ataatggagg aaagaacatg
    11581 atttttgctt ataacaaagc acacgtcgga gacaccttga tggttattat agccgacgat
    11641 aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa
    11701 ggtcaagttg ttgcttggaa ctttttccat atctccgatc atctaacaat cgaagggaac
    11761 ggccaagtaa gcttaacaga tgaggaatta gcgaaattga atcgattgat caaagaagca
    11821 ggattcgaag aacagcttga agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa
    11881 acatgcgtcc cacatcctga ttctgaccat ttatcgatta ctgagacaga agtagacaat
    11941 ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg aagctggaca aaaagttgtc
    12001 gttgcaaaac caggagctat gatgccagat ggaatgatga tctggccagg aactttgcga
    12061 ggggtagata gctatggaat gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca
    12121 gcaaaaaaag gaatcttagt attgcctgag gatgccgtag tcggagaagc atttccaaaa
    12181 taaagaggtt gtgacaagtc tttgtcacaa cctctttttt attaattttc actattttga
    12241 ttttgtttta atgccgattg atcgatcgtc aattcaacag tagttgtttt ttctttcgag
    12301 ccttcatagt aagtgatttt gacagaatct cccacttttt tcttatacaa agcagattga
    12361 agttctacac cagaagaaac ttctttgtca tcgatcttag taatgacgtc gtattgtttc
    12421 aatccagctt tttcagccgg tgtagctgtt tggacatttg tcacgatgac accgttagtt
    12481 acagatgaag gaatcttcaa gatttgttct tgttgctgag tagaaacggc agataagtcg
    12541 accattgtga tacctagagc tggacgtgtc acttttccgt ctttttccag ttgattgatg
    12601 atattcacta catcgttact tggaatagca aatcccattc cttctacgct tacattagaa
    12661 gtagattcag aagtacttgc gatcttactt gagttgatac cgataacttg tccttcgata
    12721 ttaactaaag gaccaccaga atttccaggg ttgattgctg catctgtttg gatcgcattg
    12781 atattgactg tttcgttcga ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg
    12841 attcctgaag tgacagaatt tgcatattca gatcctagtg gggaaccaat cgcaatcgca
    12901 ggctcaccaa ccttcagagc acttgagtca ccaaaagagg caactgtttc gactttgtct
    12961 gaattgatct tcaacaccgc taaatctgaa taagcatctg ttccaactaa ttcagctttt
    13021 accttcgtac cgtctttcat caagacttca agaccttgct ggccatctac tacgtgatta
    13081 ttcgtgacta cataagcagt attcccgtct tttttgtaga tcacgccgct tccttcacta
    13141 taagcttcca aattactatc atctgagctg ctactttctt cttgctgccc gaatagtccg
    13201 ccaaaaccgc ttgattggtt ttgactttgt aagttgataa cagaaacgac agcgtcttga
    13261 actttatcta ccgctttggt gatatcagaa tctacattga ctttgacatt ttccacaact
    13321 gtttcaccgg cagaattctg attgccgctt gtagcagtag aactattgtt gccagtgccc
    13381 atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa cgatccctcc aacaagcccc
    13441 agacctaact tacgccagag gctattattt ttactttttt tcatctttgg tgtcacattt
    13501 tttctatcca tgtgttcctc ctccaattac attcatttat ctactgttag tatagtctta
    13561 tttgttgact ttagtctact ttgagactgt gaacttttta tgaaaaactt taggaaaaat
    13621 cattgttttc agatgaaaat atttgatttt ctttttccac agatactgtg taaaactacg
    13681 cggataggat tggaaaaata agggataaaa catatccaca gcctgtgtat aaagtggata
    13741 aattcttata aaacgtatgt ttgcctaatg aatgtgatga aataaggtaa aataacactg
    13801 ttttctgtgg ataaacctgt ggatgttgtg cataactgtt ggggaaatct ttttaacaaa
    13861 aatagccaaa ataaacatat agacgaaaaa attgattata ataaacaaag tgagcatgaa
    13921 gaccaaagga gagaaaacat gaatatcaaa attatctcag taggaaaatt aaaagaaaaa
    13981 tacctgatcc aaggtatcaa tgagtatgtc aaacgactga atgcctatgc aaaaatcgaa
    14041 ttgatcgaag ttcctgacga aaaagccccg gagaatttaa gcgaagcgca aatgagacaa
    14101 gtaaaagaaa aagaagggga acggatctta gcgaaaatca aagaacaaga atacgtctat
    14161 gccttagcaa ttgaagggaa aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa
    14221 ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg gcggctcttt aggactcagc
    14281 gaagccgtca tgaaaagaag caatgctcag atctcttttg gaaaaatgac gtatccacat
    14341 caattgatgc gtctgatcct tgtcgagcaa atctaccgtg ccttccggat caatgcgggg
    14401 gcgccttatc ataagtgatg cggtttttat cattgatcct aaaaatctta gcaagacaaa
    14461 aagtacgtaa gaaagtaaag actaaatact tcggaggaac aaatgaataa caatgatagg
    14521 ttattgcgat tgcgttatgc gattgatttg aaagatacgg atttgattaa agcttttgag
    14581 ctaggcggtg taccactgac taaagaagaa gctcaggcgg tactgaccaa agtacaggat
    14641 aagaacaaag ataatgcaga aaataatgtg tatgaaaaaa ccatcaacaa ccaagttttt
    14701 gacgcattcc taaatggcct gattctcttg gcgcgtggtc cgcaaaaaga taagccgata
    14761 gtagacacgc cgcaaaaaag caaggaaatc aaatatatca acaatgtgtt gctaaaaaaa
    14821 ttaaaaatcg ccttgtctat gaccactgat gatattttag acgtttttgc tgaggcagaa
    14881 atctatccat caaaaggcga aatcggggca tttttgagaa aagaaggcca acgaaatttt
    14941 aagccttgcg gagataaata tatgcgtaat tttctcaagg ggttaggaat ttataatcgg
    15001 agaaaagtat aaagaaattt catgagaaat caaaacaatg gttgttttga tgagagctag
    15061 tttttgtatg agattaaaat attatcttaa tcgaggagct ggattggttt tatgaatgat
    15121 gaatataaga atgatgaaga taagatgctt tttgaagaga tagagaatcg atgtaggctc
    15181 aattttgagc ttcggggaaa gatgtcttta atacaacaaa aaaagtattt agcgaataaa
    15241 tcagagttta ctctgggaca tgttgaaaaa ttaatctcgg attggattag tagtagaagc
    15301 gaatttacaa aaataaaaca gccgattaaa tttgacatga aaaaactttt gttaaataag
    15361 agtgagatag gtaatagaga tcaatatatt agagctaaag ggcaagaaat aattgattca
    15421 ttaggtgaaa tgaggtcata caattatttg tatgtaacac atagagctga tggaatggtc
    15481 attactgtgg ggaaatcttc ttctaatgat atttttttag atggagatct tttttatcaa
    15541 ctcaatacaa atcacttgtc tggcactgaa aatataattt taaggacgga atatggcaat
    15601 gaaatatttg caaaatatga tgagatttta aaaaattatt tggactgggc ttggataatt
    15661 ccggttgagt caggagatgc taagaaactg gagagattat tgggcgatga actaattaat
    15721 aaaaaagtac caattttaaa ttattattcc catagacaat aaaaggctat ttttcatgat
    15781 aatactagtt ataactgtaa aaatggaaga aaatctggga aagaagtttt taaataagtg
    15841 tattgttcga ttttaataaa taataagcaa taccatccgt ctgtgttgcg caacatcgta
    15901 ctacaatgaa tattttttca caaaaagaaa atcatccatc gaatgaatgg aaggttttag
    15961 tatacacgag gttaatgccc cctcttttat aatcagagga gtgaggtttt ttctatgaat
    16021 gtttctattt tcttattgct agtctagttt ctcttacgat gaaattggga ttctattcca
    16081 gtttttattg atttgctgat aaaataactt gaatggtgga tataaacatg ataaataaga
    16141 gaagaataac aaaacaaggt tgtgaaatga ttctcaaatg aatataatag aaagaaaaag
    16201 gaggaagaat caatgaaaat agcaattgca ggcgctggcg caatgggaag tcgctttggg
    16261 ttaatgcttc accaaagtgg gaacgaagta ttattaatcg atggctgggc agaacatgtc
    16321 caacaaatca aagagcatgg attacaagca aattttaatg gtaaagaggt agaagcaaaa
    16381 ctaccaatcg ttcttcaatc cgaagtagaa aaagaagatc aagttgatct gattattcta
    16441 tttaccaaag cgatgcagct ggaaaaaatg ctgcaggata tccaatcatt aatcaaaaaa
    16501 gatacagagg tcttatgtct attaaatggt atcgggcatg aagatattat tgagaaattt
    16561 gtaccaatgg aaaatatcta tattggaaat accatgtgga cggcaggtct tgaaggtcct
    16621 ggtcaggtca aattatttgg aagtggttca gtagaattac aaaatttagg tgatggaaaa
    16681 gaagcagctg caaaaaaatt agcagacaaa ttgtctgaat caggattaaa tgctcatttt
    16741 tctgacaata ttcactattc tatttatcgc aaagcatgtg tgaacggaac aatgaacgga
    16801 ctatgcacaa tcttagacgt taatatggct gagctaggaa aaacatcgac tgctcataaa
    16861 atggtggcga cgattgtcaa tgagtttgcc aaagtagcag cagtagagaa gattgaacta
    16921 gatgtcccag aagtcattgc acattgtgaa tcttgttttg acccggaaac aattgggtta
    16981 cattatcctt caatgtatca agacttgatt aagaaccatc gattaacaga aatcgattat
    17041 atcaatggcg caatttctag aaaagggaag aaatatggtg ttgcgacgcc ttattgtgat
    17101 ttcttgactg agcttgtcca tgcaaaagaa gatagtttga acgtaaaata aaggagagat
    17161 tgaatggaaa cttcacaaaa aatatcaccg aaaatttttc tgaataaagt cctcgctggt
    17221 acagcaacag gaatcattat tggattgatt cctaatgcag tattaggtgc cattttaaaa
    17281 tattttagtg cgtatccctt tgcggtaacg atcgcacagt tagcagttat ttttcaacta
    17341 gcaacacctt tgatcattgg tggtttgatt gccttacaat ttggatttga tccgatgaga
    17401 atgatggttg tagccggagc ttcttttgtt ggttctggtg tagtcaaatt cacacctgat
    17461 ttgggtgaaa cgggtgttta cgttggtgct ggtacaggcg atattattaa tacgatgctg
    17521 acagcatcga ttgctgtcgg gctgatttta ctgattggaa atagttttgg gtcagtgtcg
    17581 attgtgttaa caccgattgt tgtaggtgta ggtgcaggtt tacttggttt ttatttattc
    17641 ccttttgtaa cagcaattac ttctgcaatt ggatcattga ttaataactt cactacctta
    17701 caaccgctgt taatgtctat cttgattggt tgttcctttg cctttttgat tatctctcct
    17761 atttcaacag tagcaatcgg aatggcaatc caactagatg gtgtttctgc tggtgcggct
    17821 gcgatgggcg tagctgcaac cacttttgtt ttagtagtga actcttggaa ggttaataag
    17881 tcaggtgtga caattgcgat tgctttaggc gctatgaaga tgatgatgcc taatctattt
    17941 agaaaacctg tgattcttat tccttgttta gttagtgcag tgattacagc tattcctgtc
    18001 gctctatttt ctatttcagg tacgcctgct tctgctggtt tcggcgtggt cggacttgtt
    18061 ggtccgttgg cttctttaga tgcagggcta aatggctttc ttgttgttct ttgttggtta
    18121 gtcatccctg tttttgcggc actaattact caatttttat gcgagaaagt atttaaactg
    18181 tatgatcgag aagaagtctt caaattttta ggctagtaga ttttactaag aaattttcaa
    18241 tcaatggatg cgtaagtgag atgtcaacaa catctgactt acgttttttt gcctttttga
    18301 gtaggagatt gatgctgaca actgaactct tcagtgccat tcgatttttt aaaatgtaga
    18361 agaaactcac ctttatgtta ttgattgttc aaaaggcaac tagaataaaa atatgtgtta
    18421 aaatgagaaa aaatttgatt gttggatgta tcgaataatc actaaaaagt aggagaagaa
    18481 aatgattttt ttgcaaaaat atggttttta ttttttatta ttaggtgtga ttagtgattt
    18541 atcgacaccg tatatccttg gcctttttta tcccaaactg aatcaaatga ctaccgtaat
    18601 tagtgtgttt ggtgacgtag atagtccagt cagacgtgct ttcttggtct ggtcagttgt
    18661 atctggactt ttttttgttc tttcgttgcc tgcgttgtat cacctattta ttggaacatc
    18721 aaaaacactt gcaatacttg ctgttgcaac agttggttta tatgggatag gtgattgtat
    18781 ttttactggg ttattcagta tcaatacgaa tgaaagctct tggaatctat cgacttggat
    18841 ccataatata ggttctgggc taggctatgc aggctttcta cttttcccat tgttacttgt
    18901 tctgctttat cgtcaaagtg gtcaaggtac gcttagccat ttttatcttg ttttaacagt
    18961 tattagttta ctaattgcag gtctttacgg attagcacgg attccgtcga ttagtcagtt
    19021 tgccttcttt aagcaattgg gcttctttca acgcttaagt tttttcttta actatttgcc
    19081 gatgatttgt ttaagttttt ttcaactacg aaagtagaaa aagcagtgtg aagctgatta
    19141 agtaaaagaa tttctctatg ggtaaccatt cgtgtggatg tgttcgacta agaatagaga
    19201 tgaggagagc gaaaagcaag ttagtcttaa cttgttatgc gttgtgctaa atttcaggta
    19261 gtcatttttt gaatgaataa gaagaaaact tgaaagaggg tgacttcatg ttagatgtgg
    19321 agcagatgga aatagacctt tataatatta ttttaaatcg aaatacaaga gaatgggaaa
    19381 gaacacaatt tgaagaggca aaagaagacc tagcaaaaat gtcgataaag caggtgatag
    19441 aaaaactaga ggtcaacttg cgtccgctag cactacgaga taatttgaca ccagatgcga
    19501 tggattttta tttaagaatc acaggggagt cagcgagtgc aaaggcttat gattatgcca
    19561 aacataagat aacttcaccc ttataccaag aacgggcgat ttttgccggt ggctgtttct
    19621 ggtgtatggt tgagcctttt gaaacaaaac caggtgtaat ctcagtgtta tctggttata
    19681 caggtggaga gaccaaaaaa ccaaattacg atcaagtcag tggtggatat actgggcatg
    19741 ttgaagctgt tgagatcata tttgatacac gtcaaataac ctatgaagag ctgttggaga
    19801 tttattggca aatttcagac cctacagatg cctttggtca atttcaagat cgggggaaac
    19861 aatatcgttc aattctattt gttgttaatg aaaagcaaag gcaattggct gaaaaatcaa
    19921 gagaacaact gattcaatct aaccgattta gccgtcctat cgttacagag attcgccaag
    19981 caaagacttt ttggccagct gaaaatcatc atcaacaatt ttatcagaag caacctaaaa
    20041 gatataaaaa gatcaagcat tcgcatcagc ttcatttatt ttttcagcga catagataga
    20101 ggaaggttgt ggcgcaaagc gttcagctcc gacaaaataa tgagcaactg acaaaaatag
    20161 cttttcatat ttttggacag ttatgaatta tttcgaggag ttgctttgag aacaccgagg
    20221 aaaaggttgt ggcgcaaagc attcagcttc aagtcgcaag gaaacaggag gtatgacaag
    20281 tcttttgtca caactcctgt ttttttacta gcctttattt ccatgtgaaa gagaattcga
    20341 attgtagaat cttgattttt ctatggaatt ttgtagagaa tcatcttgat aattaataga
    20401 cacctaatcg aattccacat taatgatgaa ctaaattccg tataaaagcg caaacaataa
    20461 cta
//
LOCUS       DANKHJ010000046        19451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000046 DANKHJ010000000
VERSION     DANKHJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1371
                     /locus_tag="PW113_001849"
     CDS             <1..1371
                     /locus_tag="PW113_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2526147.1"
                     /translation="VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTY
                     PDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVT
                     DVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1505..2848
                     /locus_tag="PW113_001850"
     CDS             1505..2848
                     /locus_tag="PW113_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2526148.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2960..4411
                     /locus_tag="PW113_001851"
     CDS             2960..4411
                     /locus_tag="PW113_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL2526149.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4760..5392
                     /locus_tag="PW113_001852"
     CDS             4760..5392
                     /locus_tag="PW113_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL2526150.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5980..7419
                     /locus_tag="PW113_001853"
     CDS             5980..7419
                     /locus_tag="PW113_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2526151.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7714..7932
                     /locus_tag="PW113_001854"
                     /pseudo
     CDS             7714..7932
                     /locus_tag="PW113_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8181..8396
                     /locus_tag="PW113_001855"
     CDS             8181..8396
                     /locus_tag="PW113_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526152.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8613..8869)
                     /locus_tag="PW113_001856"
                     /pseudo
     CDS             complement(8613..8869)
                     /locus_tag="PW113_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9056..9967)
                     /locus_tag="PW113_001857"
     CDS             complement(9056..9967)
                     /locus_tag="PW113_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2526153.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9986..10990)
                     /locus_tag="PW113_001858"
     CDS             complement(9986..10990)
                     /locus_tag="PW113_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2526154.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10987..13110)
                     /locus_tag="PW113_001859"
     CDS             complement(10987..13110)
                     /locus_tag="PW113_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2526155.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13115..15562)
                     /locus_tag="PW113_001860"
     CDS             complement(13115..15562)
                     /locus_tag="PW113_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2526156.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15549..15938)
                     /locus_tag="PW113_001861"
     CDS             complement(15549..15938)
                     /locus_tag="PW113_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2526157.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15998..16501)
                     /locus_tag="PW113_001862"
     CDS             complement(15998..16501)
                     /locus_tag="PW113_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2526158.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16514..16738)
                     /locus_tag="PW113_001863"
     CDS             complement(16514..16738)
                     /locus_tag="PW113_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526159.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16842..18752)
                     /gene="ltrA"
                     /locus_tag="PW113_001864"
     CDS             complement(16842..18752)
                     /gene="ltrA"
                     /locus_tag="PW113_001864"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2526160.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         6085 a         3671 c         3503 g         6192 t
ORIGIN      
        1 gtagatctaa cagataatgt tactaacttg ccagcgttac cagaaggaac aacagtaaca
       61 gatgttactc ccggcggtac aattgatacc aacacaccag gtaattacga aggtgtcatt
      121 gaagtaacgt atccagacgg tacgaaagat acggtgaaag ttccagtaga agtgacagac
      181 aatcgttctg acgccgataa atatgaacca acagtagaag gcgaaaaagt agaaattgga
      241 ggcacagtag atttaacaga taatgttact aacttgccag cgttaccaga aggaacaaca
      301 gtaacagatg ttactcccgg cggtacaatt gataccaaca caccaggcaa ttatgaaggt
      361 gtcattgaag taacgtatcc agacggtacg aaagatacgg tgaaagttcc agtagaagtg
      421 acagacaatc gttctaatgc agataaatac aatcctgaag gacaaaaagt gactacagac
      481 ttaaataaag agccagatgc atctgaggga attaagaaca aagaagattt accaaaagga
      541 actacttatg cttggaaaga gaaagtagat gttagcacag ctggaaataa aaaaggtact
      601 attgtagtga catatccaga tggatcatct gatgaagttg aagtagacgt tacagtgaca
      661 gatactcgtt ccgatgcaga taaatataat cctgaaggac aaaaagtgac tacagactta
      721 aataaagagc cagatgcatc tgagggaatt aagaacaaag aagatctacc aaaaggaact
      781 acttatactt ggaaagaaaa agtagatgtt agtacagctg gaaacaaaaa aggtactgta
      841 gtagtaacat atccagatgg atctaaagaa gaagtagaag ttactatttc tgtagtagat
      901 aaaaaagcac cgaataaacc tcaagttgat cctattacag agggtgacca aattgttact
      961 ggtaaaactg aaccaaatgc agaggtgaca gttacattac cagacggaag tcaacaccat
     1021 gggacagctg ataaaaatgg taattttaca gttaaagttc ctaaattaga ggcagggaca
     1081 aaagtgatag taactgcaac tgatgaatct ggtaatacta gtgaacctac taatgtagtt
     1141 gtttcttcca acaaaaaaga cagtggaaaa aacggtagta aaggtagtaa gactgacaat
     1201 caaggtagca acagcaatca agacaaaaat agaggcaaaa gccagtcctc aaaagttttt
     1261 cctaagacgg gagagagtga cagtaatatc tttactatta gcggtggttt aatactgtta
     1321 ggaacgttag gattattagg gtataaaaat cgaaaaaaag agaatgaata gaaaatcaat
     1381 ttcttaagtc ttctctgaga agaggaagaa tctttttctt cctctttttg cttttttatg
     1441 atgggaaata agtgttagtt aaagatgaat atacttttta tagaaaggaa aagaatgtaa
     1501 ttaagtggaa aaaatagtaa ttattggttc aaaccattca gggctagccg cagctcgatt
     1561 tctaaactta agcgattcaa attatgaagt tattctaatc gataaaaaat cagaaattag
     1621 ttacttggaa tgtgggacat cgttatttat aaaaggagaa gtaagtaatt tagaagatct
     1681 tttttatcaa aatagaatga cactaaattc tgagataaaa aagttatttc tagagacaga
     1741 agttgacaaa attgatattc aaaataaaaa gattagttgt gtaaaaaaag atggtacaag
     1801 attctctcaa gaatatgaca aagttatatt agctacaggt tcaaagcaaa ttatgctaga
     1861 tataccgggg aataatttag ctaatatttt ttcgataaag aattttccaa atgcaaaaga
     1921 gatttttaaa gctttggatg atagtactat taaaaaagta ggaattattg gagcaggata
     1981 tattggagtt gaaattgctg aagccataag aaaaagaggg aaagaagtat atcttttcga
     2041 tgttgcagat agagtgttgt cgacttatta cgaccgtagt ttttcagata aagttgaaga
     2101 aatactatca aaaaatggaa tacatctttg tctatcagaa agtcctaaaa aatatttagg
     2161 aagaaaaaag attgaaaaaa tagtcactac aagtggtacc caatactcta tggatatggt
     2221 cgtacaagct ataggatttc tatctaatag tccaattggg gaaaaggaac tcttgagaga
     2281 tgttagtgga gcatatctaa ctaatgaata ccagcaaaca aatataaaag atatatatgc
     2341 tattggggat tgtgctacaa ctttttttac gtccattaat agaagtagtg ttgatttttc
     2401 tatttctaat gctctaagaa cagcttatat tgcaactcaa catattaaca atcaagattt
     2461 tacaccttct ggttcccaat ttacaaatgg cttttctata tttaattaca atttttacgc
     2521 agcaggtttg aatttaaaaa cagcacgatt attaagtgag tctatagatt atatagaaat
     2581 agaagatttc attagacctg cttttttgag aaaaaatagt cgtgtaaaat taagaattag
     2641 ctttgaaaaa aatacaaaga gaatagtagg tgtacaactt tgtagtcaag aagatttatc
     2701 tggactatta tcaatgtttt ctcttgctat agaagaacaa attacattag acaaattgaa
     2761 aatgttagat tatttatttc atcctagttt tagccaaccc tataatttta ttttaaaggc
     2821 agtaacagga ttaaacattg aaacataatc gttactaaat gaaatcaaga aatatactgt
     2881 attactcaat tcattgaaaa tatttatcat aatttttaat agaaaacact attaaagaga
     2941 aaaaagagag gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag ttatattaat
     3001 gtcccaactc cttatagcaa cacctttcca tgtgttggca agcgaaaatt caacatacaa
     3061 aattgcaaaa gaaagaaccg agacgactga aatgagtaat tccgatgtaa cagcagagag
     3121 tagtgaactt ttgacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc
     3181 aacaacttca tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc
     3241 aagtgaaagt agtacgactt caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag
     3301 tacgatttca agtaccgaat cttcaacaac ttcaagtagt acaagtgaaa taacttcttc
     3361 gaaagtgaca aaaccaaacc atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg
     3421 acaaacaaaa actttttcga caccacagca aatagaatca acagaatctt tccaatcaca
     3481 accaatacta tctggtaatg aagaaattca ttttgacaaa aatgaatctg tagaaaattt
     3541 tattatgaaa attggtgagt ctgcgagaaa aattggtcag aaatatgatt tatatgcttc
     3601 tgttatgatt gcacaagcga tcttagaatc tgccagtggt caaagccagt tagctcaggc
     3661 accgaattac aatttatttg ggataaaagg aacttataat ggaaactttg tcattatggt
     3721 aacaaacgaa gatttgggta atggaacgct ttatacgacc caatcaaaat ttagagtgta
     3781 tgaaaactat gaggaaagct ttgaagatta tgccaaatta ttaactaagg gaattagtgg
     3841 aaataaagat ttttatgcag gagcattaaa agctaatagt aaaacatatc gagaagcaac
     3901 taaattcctt acaggacggt atgcaacaga tacacaatac tatttgaagc taaatgaatt
     3961 aattaaaacc tacgatttaa ccaactatga taaagaagtt aaaggtccac aaatgagtaa
     4021 gactggatac caagtgccta ttcataatta ttcaataacc agtttgtttg gaaatcgtgg
     4081 aaatgaattt caccgaggac ttgatatggc agctacacag ggtgagccga ttcatgcagt
     4141 taaagctggt aaggttgtta aagcagagtt tcactactct tggggaaatt acgtggtgat
     4201 acaacacgaa gatggatcaa ccgcgttata tgctcaccag caacaatacc tagtaaaaac
     4261 tggagataat gttttgcaag gacaaataat tggctatgta ggaagtactg ggaatagtac
     4321 tggcccacac cttcatttgg aaatttgcaa agatagtagt ctttctcaga gcatgttagt
     4381 tgatccgaaa accttattat tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa
     4441 gaaaatggtt ggattcttag acctttgatc aggatattga gactggaatt gatatctcga
     4501 ttacgatggg tttgacaaat aaaacaagtc cattatcgaa tatcgagtgt attattgccg
     4561 tctttatgct catattttga gcaggtttca ctagatgaaa acaatttatc tatcttgagg
     4621 atttcgtgta cgtatcgttc agtcttgtat tgtaaattag tcataaaatc agactaagaa
     4681 aaattggaaa tatttttttc tgatttatta aaagttaatt gaaattatat ggtacttctt
     4741 tcttgggagg gtaggtgcag tgaaggaaag atatggtcac cttactgttg ttgaggagat
     4801 tactcataat ggatataaaa aatatcgttg cctatgtgac tgtgggaatg aaacgattgt
     4861 atatcgatca aacttactat caggtagaac caaaagttgt ggatgtggaa attataaaaa
     4921 taggcataaa tataaaaatt tgacaaataa aacttttggt aagttagttg caatcaagcc
     4981 aactaaactg cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa
     5041 aaagttagta tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa
     5101 atcaaaaaag catcacattg aaaataaaaa attcggatta cttactgcaa agtatccttt
     5161 ggacaataac tgttcatgga aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg
     5221 tgagataagt tatagtaact tattttttgg acatactcaa agttgtggat gtttaaaaag
     5281 gcaagaaaca aggacatgtg ttgaagggac agtaatagag tgtctaacgt caaaattacc
     5341 gaaaaataat acaagtggtg ttaagggagt ttatcgttct aaagagaaat agattgcgta
     5401 tataacttta aaaaaaaaga ggtactattt aggatcgtat gattctatta agagtgcagc
     5461 tcaagctaga aaagatgctg aaaataaatt gtttcaacct ttgattgaca aatatcgagg
     5521 taatcattaa ttattgtgga taataattaa cagtgatata taaacatcta taatatatta
     5581 ttcatactat tgatatttag tataatccta tggaattgaa tatcatagca atgtatttga
     5641 aaagcaaatt ggaaaacaaa taggtgcaac accataaata tgaaaaaatt gggaaaaagt
     5701 agaatggaaa tttgcttacg tctcacgaga tagctcacac tattaatctg aagaacaatg
     5761 aagtcgactt ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag ttactataga
     5821 gtaagttcaa ataaagcatc tccaacttac atggttacat gaggatatca aatcgaaatt
     5881 actcaagctt cctatggcta gagtcgcgag aagttgttgg gcagtagaca taggttatat
     5941 tgttcttagc tacacgatca ttagaaagga aatcctatta tgtatagtta tcaagaagat
     6001 gcaaaagtcc aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt taccttcatg
     6061 tccacattgg atggcagtat tgtcaatatt gcactaccta ccatctcaga tgatatgggc
     6121 ataccaatga atcaagcgga atgggtagtg tccgtttatc tgatgattgt ctgttcttgt
     6181 ttgttgctgt ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt
     6241 acgctgatct ttgtcttggg atcgctgttg tgtgggttca atcattcgct aggtatttta
     6301 ttgttcgcac gggtggtaca agccattggt gctagtatga ctatggctac aaatgccgga
     6361 atcatcacgg aagtctttcc catacatgaa cgtggtcggg cattgggagc aataggtgcg
     6421 tttgtttcat taggctcgat tgctggacca ggtcttggcg gcttgatttt gtcccaattt
     6481 tcttgggcgt acattttttg gatcaatgta ccggtgggaa tcgtaacgat gttgattgct
     6541 gaaaaattct taccaaaaga tatcaccatg agcggccata caatcgatgg agggggcttt
     6601 gctgccattg cggctacgat tatcagcttt tttggagcaa tctttttggg gcaagaaagt
     6661 ggctttttcc atccagtccc ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc
     6721 tttttaaaag ttgaaaagaa agcagcctct ccattgattt ctttcggtat ttttaagaac
     6781 aagattttta ccatgagctt gattacagcc ttgttgattt tttcttccaa cttctttgtc
     6841 aatgtagtga ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg
     6901 ctgttgatga tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag cggctacctg
     6961 accgaccggg ttggcccgga gattttggta ttagtaggct tgggcttatt gtcggtaacc
     7021 caggtcatgt atatgttttt gtcagtggac tctcctttgt ggctatacat tgtcgctact
     7081 gccatcatgg gtgccggcaa tgccttgttc cagtccccca ataatacgat ggttatgagc
     7141 tcggtcacta tcgaaaactt gggactggca ggctcgatga attcctttgc ccgaaatttg
     7201 gggatggtca gcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag
     7261 atgggccagc aagtaactaa ctatatcgca aatcggccgg atgtcttttt atatggaatg
     7321 aagattacct ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg
     7381 cgtatcaaaa aacaaggagg tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt
     7441 tttggaggcc tgcgtatggg tgagtgggat ttccatggat gatggaagat gaagtgaact
     7501 tctaaaagcc gaaaaactca agtattatct tcaaaatact agtgctcaat gagatgcttg
     7561 atttgatcac aagaagactg cgaaagccgc tggcaaccgc aagctaggta tcccaccgat
     7621 ggactacgtt ttagaggcta ctggttatga atacggagcc aacgctctca tctgcataag
     7681 aatacatcac ccagattatg taaagatttt cgatacttct gtcaaaaaat cattaagata
     7741 gataaatgat ttccgtctag tcaaattggt tcagaatgta gacacacaga tggtaagaag
     7801 tcactcacaa ttcgagaatg gacttgtcct atttgccgta tacatcataa tcgaaacgtc
     7861 aacgatagta tcaatatttt gaccaagggc tatgaataca agcattgcct tagaaaacat
     7921 caagaacctt aggaacaacg gggatagctt ggttaataag agataccgct gtttaggctt
     7981 gcaagtatac tcttttatca agaattccgt ggctttagtc atgagaggct taatactatg
     8041 aaaattttct ttagcatttt tgatttaggt tccttttcta attataataa gatgaccact
     8101 acagtaacct actgaaatat ataaacagcc actgtagtac cattttcaaa gtagtctatg
     8161 agaggagaat cttcattctt atgaacgctg gaattgcgga tatgaattta ataaaaaaaa
     8221 cccttaatga ttttacttcc aattcaatat caaaaggtac tggtatcaat ttaagcacaa
     8281 taaaaaagct gaaaagtggt gaaagatcag tggaaaagct gaatctttta gatgctataa
     8341 aaataacgga atttgctatg aaaaatggaa aagctgagat tgaaatatgg agataattat
     8401 cgtctagtga attacttgcc acttagctga atttaacgat ccttggtgct tgaaggggga
     8461 gattcttggg aatagagcat acttgcaagc ctatgtcttt actaacaacg atatctctta
     8521 ttaaccaagc tatccccgta gttctaaggc ttttactttt atctaagcaa taatcgtatt
     8581 caaaggaaat ttttgaccct tagaaagtct cattttatca gacgtgagct taggcattgc
     8641 tgaattttgt taattcggtt ttaggttgtg ctttgagcat aatatgaaag tattctttat
     8701 cacgatttca ttcatctaag gtaatgtgat gtgattctga aattctttca aaggcagttt
     8761 ttgcataagc tgacattaca tcatcaatga catgtttaca atgtttcact actaaaataa
     8821 gggggtaacc aagaagaaaa actgaatgat tatttgtatc taattccatg gtatatcaac
     8881 catttcctat atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt
     8941 gtcggttttc gagttaccga atggcattca tcacccacct atagaggatg ggcgacttct
     9001 gcctgaaata agttacacta cttataactt ctaataaaat tagattgagt cattattatt
     9061 taacatcaac gattttccag tttccatttt tttgtaatac taatttgtac tgaaagtagc
     9121 tagtggtttt tgatacatta tcaaggtatt ttatctttgt actcactaga aaattttttc
     9181 cgttttgtag ataaactgga ttctctaact ccgaaaactt taagtcaata ttgattggtc
     9241 gcatcgcacc attttcaaca taatactcca attctttttc agaagctgat ggataaagtt
     9301 taaaaaatgt ttctaaaaaa tcggtgattt cttccgttgt tttggcatca atttctgaat
     9361 cgttttctac ttgttttgct tcatattttg ccttgtctgg ctttccaatc attgttggca
     9421 aactagttac taaatgattc ctgttttcat cttcaaagat tgtcactcga tatgcagaag
     9481 aaattgattg cgtctttttt ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa
     9541 attcttccga gtgtttttca acatctagaa tttttacaga ttgaacttcg gaactcgtgg
     9601 gaatatctgc tcgaaccata tcttggatta aagcgagacc gttttctgta aggtactgtt
     9661 cgagattact cattctcttt tcaattgctt ctttgttatt tttccatgaa aaatattctt
     9721 tcgcaaaatc ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc tcttttataa
     9781 ccactttttc atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag
     9841 caagactaca agacaataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg
     9901 atacaactcg ttcttttttc ttttttgggc tcttttcctt ttgattccgt tcaattttta
     9961 tcttcattct ttttcctctt ttctcttatt tgtatcggcc agctccgata agatgctgcc
    10021 gccaatagtt ttccgttaga ttcgtccacc ccactggatc tcctgcatga aacatgcgat
    10081 tctcccctgc gtaaatgccc acatgggtca cataagtacc ggcatcatag gttgaatgaa
    10141 agaaaactaa atcacctggt ttggcatctt ttagatcaat atgagtcatg gcatcatatt
    10201 gatcctgagc cacccgagga agtttcaggt caactttggc ataacaccat tgggtcaagc
    10261 cactacaatc aaaccccgtt tctggacttg agccaccaaa cacataggcc gttccttcat
    10321 acttaaatgc ctcatcaaaa atttcttgga ctattttgtc atcaaactga gttgtagtta
    10381 ggtattgctt cactaataaa cagtaaaaca tattgccata attataccgc cacccaccat
    10441 tttccttaat cgcgattggg ttggggtaat cgactttttg accacctgat ttttcctttg
    10501 aaaaagattc agctaaagca aaactatgct tcttcccgtt tttcccgaca tgatctagat
    10561 acccgccacc atagttatag gcctgaataa tactatcttg atcacaccct agttctttgg
    10621 aacgcttaac caaagacgca aaatagagac agccttgttt gatggatgcc tcggtactta
    10681 atgaattagg tggtaatccg gctgactcag aactttgcat aacgtcttcc attgtgccac
    10741 cagattccac ttgaataatc gccaaaagaa ttggaatata ctctgttata tccgattctt
    10801 tggcatactt ttccaccatt gcacggtgtt tcaatacttc ttctgagaca ctagttccgc
    10861 caggagaagt gccagaacta ccatttccct cactgtcttc accaaacaac aaacccacac
    10921 acatcaaaat acccataaaa gagatggtca gaaaagttaa tgaaataaca cccactttct
    10981 tcaagttcat tttttctcac ctgaccttga ttttggacga tggttcattc ttttttgtac
    11041 tttttgattg gtctgctttg gcagagtgga cctctgtttc acttgttgga aagaattttt
    11101 cttacttgct gattggcttt tcccttttga aatgatttct tgactagtta gttttcgttt
    11161 gatttcggga ttcagatttg ctctcacaat ccgctttggt gcttcttttt tcagcatcat
    11221 atctttctga ggttgagctg ctttatctat tggaatattc gcccttcttt ttccctgacg
    11281 atttgttgct tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg
    11341 ttgtttttct cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc gcttgaattc
    11401 ttctggtgct tttttcgact tttcgattcc tttatggaga ttgtatttca aattcgttgg
    11461 cgtattcctt acctgatcct ttacttgttt agtcttatct ttcaccaaag cttgggtatc
    11521 caaaactttg ccgatttttc tcccagccaa attcattcgg gattgtttct tattatttga
    11581 aactggattc tccttagtta agtcagatag tatttcgtga tcgttcggta atcgtgaatt
    11641 ctttcgtaga gaagaaccgg atggcttaaa tttggatttt gattgttcct ttgccaactt
    11701 atttccgact gctgcaccac caccaaaagc caaagctcgt cccacatttc gattgagttg
    11761 ccgcaatttt cgattcatta acatttgcgg ttttcgcatg acgcgtcttc ccattgactg
    11821 gctatcatta ctctggaggt tgaacatact cataatgtcg cccaatttga aatagatacc
    11881 ggcaaaggtc acaatttgca gaaaagcaac catgaaaaat ggataggttg ccgaaatatt
    11941 gaaaaacatc gtggagatgc taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat
    12001 cataatcgta ttgaataaac gaataatcgc cttctttcct aaactttcat aagtcggcaa
    12061 catggacaat aagaaactaa ttggcagaaa catcgcaaag ataatgaaga gaatttgcga
    12121 gaacaacata atccctgtta gaagaaaaac aaagaaggaa atgccaatat tgaacaatac
    12181 caaaaagacc acggtcccta aacggttcat ggtcttggta atactgagat tcgcattgtc
    12241 attgtcttca atctctgcct tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg
    12301 actcacagat agaatcttgt tgacccggtc ttcaccaatt tcttctatgt tggaatcatc
    12361 aaattgcagt agtaaccaag gttgttggac ttgaatcgaa aacaaactat cccgaatcaa
    12421 atccacacta tcttttcctt ggctttctga attgggtaca acgatctcgg ttccaagggt
    12481 aagggccgct tcactaacat ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc
    12541 ataagcaatg aacgaacccg atagtagaaa aatcatcaca aaattaatca cggctcgaac
    12601 tgctttcgtg gtttctcgtt ttaataatcc ggtatacgcc acatacaccc ccagagctaa
    12661 aatcatcaat aatagaaacc caaaataaaa tccggaggtc tggagtccat tttcagtaat
    12721 accagccaat gtttggatat tctttccaat actttcagcc gtatccgaaa tgaaatctaa
    12781 tttgtaagcc tcttgaacca cataacccgt ggcgttcgac aaatacaaac tcaccgtcca
    12841 aatgaaatta gtaatggcat aaagaccata catcacgctt ttccctaagc catcacccca
    12901 attccaaggc aaccaatccc aagagctatc cacaaagaaa tctagttgat agttgttcag
    12961 tgaatatttg gaatatagat ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc
    13021 ggctaccgta ccaactaaac tcaatagaac tatcagagtg agtatgctaa taaaaacgat
    13081 tcccacaatc cgaaaaagtt tctttttcat cccctcactc cattctctct tgaacaggtg
    13141 gacgggtatc aaaggcatgg aagagttctt caaagactgg atgtacctga accacgccta
    13201 cccgaccata caaatcctgc atcaagcatt ggccattttc caaatctcgt aggcgttttt
    13261 gattgctttc atcttctcga tccaagccaa agaattccaa ggttttcttg atttcatgaa
    13321 tatcggtact gcgaaacgca aacttcaacc cgatattatt cttcaagcgt tcatccagta
    13381 aatcatcact attttgcgtg acaaagtaaa ccccggcatt catagcccgc ccagcacgaa
    13441 cgagcttatt cgataatgtt ttcccttgcg ctacttgaag aaaactccac gcctcatcta
    13501 agtctacaat tttgaagatt tctcgattac tatggataaa gtccaaggca aaagtggaaa
    13561 tgacgattag catggaaaca ctaagcaatt ccatggtggt atattcttca aaagtggttt
    13621 ctgcatctgg taaaaccaaa tccgctactt gaatgatatt taactgccta tctaaactaa
    13681 tcgactgggt aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg
    13741 actcgatgtg atccgcgata ttttccgcaa ctggtgagcc ttcttttcga agctcattaa
    13801 ttacttgtaa taatccccgt tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca
    13861 ctggaaactt ctccccatct cgactagaaa ttcctgttaa aaaggtcaga atatcaattg
    13921 ctagactttc agaatccttc tttttctcca taatcacata aggatcaagt aatccttgat
    13981 tttttggctt actagttaag ttgagaatat tgatttcatc cgcaatttct ggcaaggttt
    14041 ctttccagcc accccgttcc ccttttggat cgacaatcac agcttgtcca ccaaaaagga
    14101 cggcgtaata aactaagaga ttgttactaa aggactttcc gccaccgagt gaaccgagga
    14161 atgccgctgc tagcgcattg gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt
    14221 tcaggtaaac attgcgcccc gtatctacgt tataaccaaa gtaaatgcct tccaactccc
    14281 ctaacatctg agtggcgcca aaaccaagtc cggcgagaaa atcagacgta acatactgga
    14341 tataatcatt catgtatcgc ttactagaag gtaagaattc ttcatgtagc cccatcatat
    14401 cgccaaaagg ccggactagt tttacattgg tatcatcata aaaatctagc acttcatcac
    14461 accggcgctt tagttcatct actgattcgg cacttactcg aaccacatag ctcagtttgt
    14521 acatggattc tttggactga tccaaggtag tttccaattc atctacggaa tccaatgcat
    14581 ctaagacatt ggaatttgtt tcattgtcag attgataggc atggttgtcc aagtctttta
    14641 attccttttt cttattgcga acggtagcaa gtgccttttt gttcgtcaca atctccacat
    14701 tcatactagt gtcaattggg aatgtaaatt gttgttgctg gtaatagaaa agttctgagg
    14761 agggaaactc catttccccc acaattgtat taattgttaa gtacgctaca tatgattcat
    14821 gattctcatg ttccatgcat aagtaccggg gcttttcttc aatcaaacaa cgactgggac
    14881 gtaataagtc gtaacgtttc accaaagttt ccgacttcag ctttttcttt ggtagctgaa
    14941 attcatattc ttcaaatggt gtaccctttt cgccatagat atgttcaatc aaatacatca
    15001 gatcactcgg tgtgactcgt ctgactttaa atcttcgagc caatttactt tccattagtt
    15061 tttcaagctt ggtatatcgg cgaatttctt cattggataa ggaaacaaaa tcgcccatca
    15121 aatgatgatt cactccatag acaaattctt gaaagccgga gaagaaagat ttcttgagat
    15181 tctttagatt gacctcttca tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat
    15241 caatctgact atcgccaatc atggatacta atgcctctgt ctgtaaatca attcgttgtt
    15301 tcgctacttc tgttaatcgc ccggtaattt ccttttttga tcgttcttgc gtggcacgaa
    15361 tactactttc tgtggcaatt tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca
    15421 attgacgaaa gttatcgtgg acttgatatt tctgttcagg tgacaaaaag gagtaattgt
    15481 aaggtattaa ctcatagtag gcaaaacatt ctccctcttg attaaagaca agattatttt
    15541 caatatactt aatcgggtac atagttcaca ctccttacca gagtaatggg ttcgtcccat
    15601 cgtttcttct gttctttgac tttctttcct gcgaaagtga ccttaaatcg taagtgatag
    15661 ctaataaaag atcgtaaaaa tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg
    15721 aaccaagtaa ctgccaccgg tatacctaag tattttaaaa aggccccctc tatcatagat
    15781 agtggaggca catgggcaaa tacgatgaca aacagcaagg ataaaacaaa ccaagccatc
    15841 tggttaaaag tcacaggaaa aggcagttga aagtcattaa ttgcataaat gaccttttca
    15901 actgcccaaa tactggtgta gctttttatt ttcttcatca aatcattcct ttaaaaagaa
    15961 ggatagttta gaaatcaact acccttcaat aattttgtta atatggatac tcaaaaacac
    16021 ctcgactcgt taccaaataa ttgcctccaa tctccaagtc tctaccacac gcatcaaaat
    16081 caatatacgt cttcaagtgt tctggtactt ctccaaaatc accagcttct acaatcaaga
    16141 aatatgctac atcactcata tcatcgcaat ctggataata gataatatca tccacatgct
    16201 ctaccatctc ctcaaaagaa ttgaagaaag catgttgaat ctcagaagca acttcaccaa
    16261 ctggcgttcc ttcaagttcc tccgctaagc cacacaaccg attaatttcc tcaattggcg
    16321 tatactcatc aatctcaaag ggaagttcat aatcatgaat tgcatattcc tcgtattcgt
    16381 catttaaccc aattcgttct ttcacttcat caaaatcgac aggtggggta aaccacgccc
    16441 ccaccaattc accttcgtta tatttcccga ggttcgcaat ataaacacgc atctgttcca
    16501 tcgtgcttcc cccttacgca ccgacaattc ggttaaacaa actgagtaag acatccttca
    16561 ccccagaagc attaaagacc aatccgaccg caatcaaagc aatcaccaaa aagccaatca
    16621 gtttggaaaa ctcccgctta aatcctaaaa acaaaccaat gaccacaatt gccagtaaga
    16681 ctaaggattg ggcattggtt aaaaaccatt gatatatgga taggtaagtt cctgatatta
    16741 taaatatctc aaacttttcc ccccattcac acggagcgtg cgactttcac cgcactccgc
    16801 gttccatcgt gtttatattg aaaagtattt atgtagtaga cctatatcaa gtttaattct
    16861 tgattgccag ctaatactct atatttattt agtcttatga gctggacttt atttagcttc
    16921 aatagagcta tatatttttg tattgtttct tctgttttag cgtgtattag acggtgtacc
    16981 gcctctttta ccaaaataag atttttataa tcgtcatttc caccagctcc agttggtaca
    17041 atatgatgac aatgtatttc attagcttgt tgaaattctt ccagagttat agcacatttt
    17101 ccatgttgag cagaaaatag cgaaattcga ttgtctgcgt attctattgt gcgattttcc
    17161 aaagataatt tcattaactg gtacatcagt aatttatcaa cacttagatt ggtgtggata
    17221 aattttcttc cctctagagt gtagcaattc gctaaataca cctttgccat tggttttctg
    17281 tgttggatat atccaacagg gaaaataggt tcgccctcgc cagcaacata ccgaagcatt
    17341 ttagatttac catatcttgt tgcctccttt gtcgtaagtt gtctgcctgt ttttctcaat
    17401 ctgccccttt tgtttgtacc taatctgtta gtgaagatag ccattacagc tctattcagt
    17461 ttcatacaat ccagatttac attcgttgca atctgataat aattttgcat acctaatacc
    17521 attgaattat aaagtcttgt ttcttctaat tccgttttcc cttttgaggg agaagcaatt
    17581 ttctttgcct gtgataccag cttgtctttt tggtttttga attgtttatc acttatatgt
    17641 gatttaacaa ctaacttctt tcttctcggt atcattttaa tcttgaaacc aagaaagtcg
    17701 gagtatcttt ttctaacatt tacaattctt gttttttctt gtgatacttc caattttaat
    17761 cgttgttcaa tccattgagt gattgctatt ttggcttttt cagcactttc cttgtagcgg
    17821 cagaaaactc taaaatcatc tgcataacga accatataca tttcctttaa ctttgttttc
    17881 ttcatttctt tgtaagcgtt gcttttacat gggctaccgc ttttattgat atgaaccaca
    17941 tagttttttg taacaggatt ttcctgccat tggctttcta cccaatggtc taactcatta
    18001 agaacaatgt ttgctagtag tggggatatt attccccctt gcggagttcc tttctctgga
    18061 tatgttattg taccgttagt cattttgatt ggagctttta atatcctttt taaaatgaat
    18121 atcaagtgct tgtcccgtat tcccatagcc catatctgtt ttatcagctt ggaatggttc
    18181 acgttgtcaa agaaaccctt aatatcaaat tcaatcacat aatgtagctg gctgatttgt
    18241 aggcggttat aggttgcttt tattgcattt tcaactgacc tattaggtct aaaaccatag
    18301 ctgttttcac taaatttagc ttcgcagatt ggttctaata cttgttttat acattgttgt
    18361 ataagcctgt cccacataca aggaatacct agaggtctgg ttttaccatt tggttttggt
    18421 atttcttttc gcctcactgg cttaggtcga tacccatgtg gactgcctgc aacaatgtat
    18481 cttacttttt tgacaactgt ttctgctggc aatttgccaa tatctttaat tattgtaccg
    18541 tctgttcctg ctgttttact gcctgcattt gctttgatat ttcgatatgc cagtaatata
    18601 ttttctctcg ataggataat atccataaga tgagtgaaaa tatctccatt cttacttttg
    18661 gcatataaat catcaaaggt gttttgcatt tcgtaatatt cagcgtgtct taaatcttct
    18721 acacacaatg tttcattttt atttttaggc ataaggcttc actcctttac ttgaaattga
    18781 gtgagctttt ttatcttact cgtaatcctt attgaaacaa cataatactt ttctaatata
    18841 tacacgactt gagggcattc ctccattctc attacaagaa tttcttaggt tcgccctctg
    18901 cttttcagca ttagttcaac tgcttattgt tcttcgtcag tttattctct tgatgagaac
    18961 ccaatacctt tcctcgttcc gataatctta tctatacata tatatcctta ggtcactagc
    19021 tataagcctg ctggcttgat accgcctgta acgttatatg gcatttcata acctatattc
    19081 ttactttgcc acaccagcta cgatttctcg tacctttgca tttctacaaa gttcaaattt
    19141 agacccgtac attcgctgtt tcgtcagttc cttatagaac attctaacca tagatatttt
    19201 atagaccccc gacctgttgg atacgctacc cacctctgga tagttttcga tagaagatat
    19261 atgttcatca actattacgg tatctttcag ccgtctttac cgagcttcaa acattctgag
    19321 tttcctcata caatgcttgt cggaggctca gggcaggcgt ttcagagcgt tacttcatca
    19381 ttccacctgt cagattatca gttctcctaa taaccaaact ttttcgttct tttattagtg
    19441 ccttagcttt t
//
LOCUS       DANKHJ010000047        19384 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000047 DANKHJ010000000
VERSION     DANKHJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            268..1746
                     /locus_tag="PW113_001865"
     CDS             268..1746
                     /locus_tag="PW113_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526161.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2023..4083
                     /locus_tag="PW113_001866"
     CDS             2023..4083
                     /locus_tag="PW113_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2526162.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4166..4735
                     /locus_tag="PW113_001867"
     CDS             4166..4735
                     /locus_tag="PW113_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526163.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4910..5716)
                     /locus_tag="PW113_001868"
     CDS             complement(4910..5716)
                     /locus_tag="PW113_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526164.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5854..7368
                     /locus_tag="PW113_001869"
     CDS             5854..7368
                     /locus_tag="PW113_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2526165.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7771..8754)
                     /locus_tag="PW113_001870"
     CDS             complement(7771..8754)
                     /locus_tag="PW113_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2526166.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8759..9406)
                     /locus_tag="PW113_001871"
     CDS             complement(8759..9406)
                     /locus_tag="PW113_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2526167.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9491..10123)
                     /locus_tag="PW113_001872"
     CDS             complement(9491..10123)
                     /locus_tag="PW113_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2526168.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10139..10690)
                     /gene="coaC"
                     /locus_tag="PW113_001873"
     CDS             complement(10139..10690)
                     /gene="coaC"
                     /locus_tag="PW113_001873"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2526169.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10687..11424)
                     /locus_tag="PW113_001874"
     CDS             complement(10687..11424)
                     /locus_tag="PW113_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2526170.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11421..12410)
                     /locus_tag="PW113_001875"
     CDS             complement(11421..12410)
                     /locus_tag="PW113_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2526171.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12668..13210
                     /locus_tag="PW113_001876"
     CDS             12668..13210
                     /locus_tag="PW113_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526172.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13227..14312
                     /locus_tag="PW113_001877"
     CDS             13227..14312
                     /locus_tag="PW113_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526173.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14324..15130
                     /locus_tag="PW113_001878"
     CDS             14324..15130
                     /locus_tag="PW113_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526174.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15131..15958
                     /locus_tag="PW113_001879"
     CDS             15131..15958
                     /locus_tag="PW113_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526175.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15955..17028
                     /locus_tag="PW113_001880"
     CDS             15955..17028
                     /locus_tag="PW113_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2526176.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17456..>19384
                     /locus_tag="PW113_001881"
     CDS             17456..>19384
                     /locus_tag="PW113_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2526177.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKM"
BASE COUNT         6344 a         3371 c         3906 g         5763 t
ORIGIN      
        1 gcaggacggt ggccgtcctg ctcttttttg tggttgttta cgcaaatggc gcaaatcttg
       61 tacattttaa cacaataaaa caaaaaatat agaatatttc ctcataaatt tttccatttt
      121 ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa acaagcataa
      181 tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc aagtaattgg
      241 tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga agaggaagat
      301 agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta tgtagtgctg
      361 cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta tatcatagaa
      421 ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc agaagaagca
      481 gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag aatctacttt
      541 caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt aacgatcgaa
      601 aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag aagagaatta
      661 gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt tggggaagaa
      721 ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg tttttctatg
      781 ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt tgaatatctg
      841 ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac cactattagt
      901 ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt
      961 aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt tcttcatatc
     1021 cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat cacggaagaa
     1081 gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga tatccacatg
     1141 attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat tgaaatctct
     1201 gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga
     1261 aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca gctacagttt
     1321 ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca agagaataaa
     1381 cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga ttatctcttt
     1441 ttattggtca gtatttgtcc ggtatcatat tttgaagcac cgattcatgt ctgtattgat
     1501 ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg atttaaaaat
     1561 tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt atctgattgt
     1621 ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac tccagatgac
     1681 tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac tgaaaaaatc
     1741 agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt tatgtttttt
     1801 tcaaagaaaa ggtaactaga tataactaga tataactaga tatattactt aacaaaatta
     1861 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
     1921 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
     1981 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
     2041 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
     2101 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
     2161 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
     2221 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
     2281 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
     2341 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
     2401 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
     2461 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
     2521 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
     2581 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
     2641 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
     2701 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
     2761 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
     2821 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     2881 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     2941 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     3001 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     3061 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     3121 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     3181 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     3241 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     3301 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     3361 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     3421 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     3481 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     3541 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     3601 caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     3661 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     3721 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     3781 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     3841 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     3901 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     3961 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     4021 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     4081 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     4141 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     4201 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     4261 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     4321 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     4381 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     4441 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     4501 gaaatcagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     4561 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     4621 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     4681 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     4741 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     4801 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     4861 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     4921 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     4981 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     5041 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     5101 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     5161 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     5221 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     5281 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     5341 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     5401 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg
     5461 ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta
     5521 atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg
     5581 tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta
     5641 ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg
     5701 ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt
     5761 gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac
     5821 ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta
     5881 acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag
     5941 acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat
     6001 acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca
     6061 gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag
     6121 atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca
     6181 aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta
     6241 aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct
     6301 gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga
     6361 gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta
     6421 gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat
     6481 gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga
     6541 gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc
     6601 agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa
     6661 gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat
     6721 atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag
     6781 gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa
     6841 atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat
     6901 ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa
     6961 ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa
     7021 gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct
     7081 caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc
     7141 aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa
     7201 gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt
     7261 ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg
     7321 cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa
     7381 ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca
     7441 ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     7501 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     7561 aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
     7621 tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca
     7681 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
     7741 gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc
     7801 gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt
     7861 acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa
     7921 agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag
     7981 ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag
     8041 cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta
     8101 agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac
     8161 ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca
     8221 aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct
     8281 agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc
     8341 gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga
     8401 gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg
     8461 catcacagca tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg
     8521 agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa
     8581 aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac
     8641 agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc
     8701 aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct
     8761 attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta
     8821 ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg
     8881 tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa
     8941 cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt
     9001 tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt
     9061 ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt
     9121 cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt
     9181 gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca
     9241 cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg
     9301 aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca
     9361 ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt
     9421 cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct
     9481 acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata
     9541 ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg
     9601 ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct
     9661 ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc
     9721 cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat
     9781 aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt
     9841 tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag
     9901 gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag
     9961 cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg
    10021 cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag
    10081 ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct
    10141 aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta
    10201 aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat
    10261 agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg
    10321 tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg
    10381 aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg
    10441 cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg
    10501 attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg
    10561 tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga
    10621 attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga
    10681 tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga
    10741 tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc
    10801 gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact
    10861 attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat
    10921 gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa
    10981 aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc
    11041 attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc
    11101 cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat
    11161 aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc
    11221 agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt
    11281 agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc
    11341 taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc
    11401 tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg
    11461 tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg
    11521 acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga
    11581 tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc
    11641 aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta
    11701 taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag
    11761 tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg
    11821 acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca
    11881 atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca
    11941 gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc
    12001 acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata
    12061 ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct
    12121 ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt
    12181 ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt
    12241 cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag
    12301 gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct
    12361 gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca
    12421 ctccttttaa aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc
    12481 gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa
    12541 aagataaaga aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt
    12601 cgtatactaa tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga
    12661 ggaaattatg gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca
    12721 agaagaacta ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga
    12781 tttaagttca ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc
    12841 agaagaattt tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca
    12901 tacggtttat ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa
    12961 tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt
    13021 tgaaccttca ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt
    13081 aggagagacg gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca
    13141 acatcaatta aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc
    13201 atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag
    13261 tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa
    13321 aattttatac actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag
    13381 ctggattcac cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt
    13441 tgccagctaa taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata
    13501 tgaatgtttt tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata
    13561 tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg
    13621 agattagtga aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa
    13681 atgaaccgaa agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta
    13741 cagatatgca gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg
    13801 ttactcatga ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag
    13861 ggaaaatcgt ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg
    13921 ttgctgattt tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag
    13981 ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag
    14041 ttgaagttgt tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag
    14101 tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg
    14161 aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag
    14221 gattggcctt tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat
    14281 tcgatgcacg tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag
    14341 gatctattcc gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat
    14401 gatcatttat caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc
    14461 ttattttacg tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt
    14521 gatcacgttt ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa
    14581 gcacaagcaa ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa
    14641 agcttacgca tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt
    14701 tatcgggatc gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac
    14761 gtatatcgag attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc
    14821 ttctttgatc agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt
    14881 gatttttccg ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc
    14941 tctttcttta ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac
    15001 ggctatcgaa cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt
    15061 gattttaatc attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg
    15121 tcgctcataa atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt
    15181 cctatatgca ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa
    15241 cagctttaca ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat
    15301 cattatcgta ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat
    15361 cgggacattt ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt
    15421 gttgagcatg aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt
    15481 gattttcttt acactactag gattcggatt aggttttcag agtgtattgc tttcccatat
    15541 tgctttcagc attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga
    15601 ttcaatggtc gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat
    15661 cattttgcct tatctttctc caggaatcat agctggatat tttatggcat ttacctattc
    15721 tcttgatgat tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt
    15781 agagatctat tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt
    15841 agttttcttg ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg
    15901 tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa
    15961 aaattacaat ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt
    16021 cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg
    16081 ggagattata tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg
    16141 aattatgaaa catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca
    16201 aactacgaca tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta
    16261 gtacttccat tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta
    16321 gacttggatt ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg
    16381 atcgtatata acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg
    16441 aagccggaat taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt
    16501 tctttgaata gtttaggcta ttctttgaat agtaagaata tggcacagct gacagaagca
    16561 gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa
    16621 atgtatatga tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat
    16681 atgatgtggg aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg
    16741 tttgataata tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc
    16801 aactttatgc tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca
    16861 ccaaatgaag aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat
    16921 ccatctgatg acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta
    16981 ggcatctata atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca
    17041 gtaagaatgg ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag
    17101 ttttccaatc ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt
    17161 tctaccacgt ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga
    17221 cgggctagta aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc
    17281 aaatatactt aacccttctg taatctatta ataaaactaa tatatgaatc cagttcaata
    17341 tttgtagcta tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat
    17401 tattttgtca gatatctgag gcaaatattc gactatttgt gtttcaaagt aaaaattgga
    17461 atacaaatat agaatagaca tattgcagcc gatagtttat tatgctttta gacacattaa
    17521 actggaggaa aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc tgatacgact
    17581 aacagggaat atgtcttctg tcactgccga gaaaatttcg aagaatctag gtatctctcg
    17641 tcagaatgcc agccattatt tgacgagatt agtggaagat aaaaaggtcg agaaattacc
    17701 gggaaagcct gttttgtgga agcctttaga tgaatatgcg gttgtagata atacggagca
    17761 aataaacgaa gcctttcatt ctgtagttgg tcatgacgga agtctgagag aggtcattca
    17821 aaaatgtata gccgctgtca aatatccgcc taacggactc agtgtattaa taaatggagc
    17881 cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt gaatacgcta tccatgagca
    17941 aattattgaa aaagatgcac catttgctat cttaaactgt gccgattatg cggacaatcc
    18001 cgaactgctt tcggcaacac tgtttggata caagaagggg gcctttacgg gggctgaaaa
    18061 ggataccgat ggtttattgg caacggccaa caacggctat ctttttctcg atgaggtcca
    18121 tcgcctatcg aaagaaaatc aagaaaagct ctttctattt attgatactg gaaactatcg
    18181 tccagtcggt gaaaatgtaa attggcattc tgctaaggtt cgttttattt tcgccacgac
    18241 ggaaaaaggt gaaaattatt tattagacac ctttgatcgg cgtattcaaa ttagtgttat
    18301 gttgccaact tttgaagatc gccccattcg agaacgatta gagctgattc aattattttt
    18361 tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt tcaagagaag ccttgtcttt
    18421 aatgttatct catcccttta gtgggaatat tggtaagctg aagaatatca ttaaaatcag
    18481 ttgtgcggat gtgtatagtc gaacacaaga agaaccatta acgattggga aaaatgagat
    18541 cttgattcag ttaaatatgc tggaatccga agtagaaagc ttgccgattg ctagtttatt
    18601 gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat
    18661 tttggaaaaa attactcagc tgataaatga aggtaatttc aatgaaaatt tttcttcgat
    18721 aaagaatgaa gtacagcatt tggtgcatca cattgataag gattcttttt tatcaatgag
    18781 tagaaaattg atgattcaga tgtttagaaa ggtctgggta tccgttttag gaaaaacgta
    18841 cggattgacg acgagtggac ctgttgcaga aattattagc agatcctatg tcatttcccc
    18901 aagctataag acagacttaa ccgggataaa tcgtacatta gctgcaaatc tacctcggac
    18961 gacttactta tgtaatcagt tcttggagca tttaccaccg ctctcagaaa aggacgagaa
    19021 ctttctaaga ctgcttattg tcgtgacttt atctgattat gtagatgaat ccatcgagct
    19081 gaagggcctg ttgcttgctc acggagatag tacggctagc agcattcaag cagtagtgaa
    19141 tcaattatgc ggcaattatg tttttgaagc gttggatatg ccagtagaga gtaatgtgtc
    19201 agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag atagtctagt
    19261 gctgatcgta gatatgggct ctttaaatca aatttacaca cagatcaaga acgatttaaa
    19321 aggggagtta cttcttctga ataatttgac gacttccatt gcacttgata ttggcttgaa
    19381 gatg
//
LOCUS       DANKHJ010000048        19250 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000048 DANKHJ010000000
VERSION     DANKHJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..578)
                     /locus_tag="PW113_001882"
     CDS             complement(<1..578)
                     /locus_tag="PW113_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2526178.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLK"
     gene            complement(579..1466)
                     /gene="hslO"
                     /locus_tag="PW113_001883"
     CDS             complement(579..1466)
                     /gene="hslO"
                     /locus_tag="PW113_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2526179.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(1683..3794)
                     /gene="ftsH"
                     /locus_tag="PW113_001884"
     CDS             complement(1683..3794)
                     /gene="ftsH"
                     /locus_tag="PW113_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2526180.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(3884..4429)
                     /gene="hpt"
                     /locus_tag="PW113_001885"
     CDS             complement(3884..4429)
                     /gene="hpt"
                     /locus_tag="PW113_001885"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2526181.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(4429..5874)
                     /gene="tilS"
                     /locus_tag="PW113_001886"
     CDS             complement(4429..5874)
                     /gene="tilS"
                     /locus_tag="PW113_001886"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2526182.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(5994..6464)
                     /locus_tag="PW113_001887"
     CDS             complement(5994..6464)
                     /locus_tag="PW113_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2526183.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(6510..6935)
                     /locus_tag="PW113_001888"
     CDS             complement(6510..6935)
                     /locus_tag="PW113_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2526184.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(7002..7271)
                     /locus_tag="PW113_001889"
     CDS             complement(7002..7271)
                     /locus_tag="PW113_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2526185.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(7282..8877)
                     /locus_tag="PW113_001890"
     CDS             complement(7282..8877)
                     /locus_tag="PW113_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2526186.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(8892..12413)
                     /gene="mfd"
                     /locus_tag="PW113_001891"
     CDS             complement(8892..12413)
                     /gene="mfd"
                     /locus_tag="PW113_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2526187.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(12430..12990)
                     /locus_tag="PW113_001892"
     CDS             complement(12430..12990)
                     /locus_tag="PW113_001892"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2526188.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            13343..14317
                     /locus_tag="PW113_001893"
     CDS             13343..14317
                     /locus_tag="PW113_001893"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2526189.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            14487..15887
                     /locus_tag="PW113_001894"
     CDS             14487..15887
                     /locus_tag="PW113_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2526190.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(15965..16687)
                     /locus_tag="PW113_001895"
     CDS             complement(15965..16687)
                     /locus_tag="PW113_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2526191.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(16766..17272)
                     /locus_tag="PW113_001896"
     CDS             complement(16766..17272)
                     /locus_tag="PW113_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2526192.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            17395..19194
                     /locus_tag="PW113_001897"
     CDS             17395..19194
                     /locus_tag="PW113_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2526193.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5417 a         4215 c         3472 g         6146 t
ORIGIN      
        1 tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt acgtccatgc
       61 atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac agcaaagacg
      121 tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac tgctgaagaa
      181 acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc tcctgcttct
      241 gctttgatta ctttatttac tggacagccc atattgatat ctattatatc ggcagctgtg
      301 ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc aaatatctgt
      361 aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt tttgtttctg
      421 aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc aaattctttg
      481 acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac gacacgatta
      541 gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc taaggcttct
      601 ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca gaaagaacaa
      661 accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc acctaacgcg
      721 ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac aggcattttt
      781 tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc tggtgtctgt
      841 ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc gatcttgctg
      901 attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc cgtcttgatg
      961 ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg ttctgagata
     1021 gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt ttgcccagaa
     1081 aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt tccaactgct
     1141 ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg atttttgatg
     1201 taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc tggaccatcg
     1261 ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag tagcagacta
     1321 ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg acgtctttgt
     1381 gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc gtcgtgagca
     1441 agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt tttaaactca
     1501 tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct tttaaaaata
     1561 attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata aagaaatctc
     1621 ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg acctatattt
     1681 ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc tgaatcatgt
     1741 tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg tttttcttcc
     1801 gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa agttttcgct
     1861 tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc gtgttcaaac
     1921 aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc agcaatcagt
     1981 ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc ttccatcaag
     2041 atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc ttttgtttga
     2101 ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg acctaaacga
     2161 tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg ctcaaagtca
     2221 ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg tccaccaagt
     2281 aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc ttcttttggt
     2341 aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt gtggacgacc
     2401 cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata agcaaccatt
     2461 tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat cacgcgatct
     2521 tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc agctactaac
     2581 gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt ctgttgagcc
     2641 acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac tcgcaagatt
     2701 gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa acggcctgga
     2761 cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac gattactcct
     2821 tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg ttcgcgttca
     2881 tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat ttcatcaatg
     2941 aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac acgacttgct
     3001 ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa cggtactcca
     3061 gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg tccttctaaa
     3121 agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc tttcagaaat
     3181 tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc agaaaaacgt
     3241 acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt catgacacgg
     3301 ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa aaagatgatg
     3361 attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct tgattcttct
     3421 tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct caatgttgta
     3481 tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga acctaagata
     3541 gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt gatcttatat
     3601 acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag ttgttctgtg
     3661 aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat gaaataaacg
     3721 accattacca tcgctaagat tacgaggaca taatacaagg catttttcat catgccgttg
     3781 ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt tttttatcaa
     3841 tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt gattgataaa
     3901 cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca taatccaagc
     3961 cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct tcgatattta
     4021 ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt gctttgcggt
     4081 atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata tcttcaacga
     4141 tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt acttcccctg
     4201 acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc aaatacgtat
     4261 cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca cctactacta
     4321 ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg caacgaactt
     4381 gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc aggttctcct
     4441 tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat cagtttcttc
     4501 acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc ttttggcatc
     4561 tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga taaaatatct
     4621 agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt ttcttttttc
     4681 gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag accatgaatc
     4741 tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt ctgtttcatt
     4801 caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct ggctagttgc
     4861 ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta tccaattatt
     4921 gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa tcgactccat
     4981 ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa aggagtaaaa
     5041 aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa atcgaaattg
     5101 ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt gaatgacttg
     5161 gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct gtgtattttc
     5221 ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat ctaacaattg
     5281 gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat ataggtcttc
     5341 tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt gtacttcttt
     5401 gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca ccgtctcgat
     5461 ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt ccatcacgcg
     5521 agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt tggctggatc
     5581 ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc gaagataggc
     5641 agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac ctaaagaaaa
     5701 accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat caacaccacc
     5761 tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat tcccgtgccg
     5821 aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt tcatcctttc
     5881 cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac aattaacctg
     5941 ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc tttttagctg
     6001 cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga agataagcgg
     6061 tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt ttgagctgaa
     6121 ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc acggggatct
     6181 ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg ttcctgcgct
     6241 ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt aactgttact
     6301 tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga cacttcgcta
     6361 atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa atttgtgatt
     6421 cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa aatgttcctc
     6481 ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact agttgattga
     6541 tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac tttttcaccg
     6601 tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc atctttcagc
     6661 aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat ttcagtattt
     6721 gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa aatctgcaca
     6781 cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct gcgtcttcga
     6841 aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc atattccgta
     6901 tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa tctttttgat
     6961 tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc tagtgtttct
     7021 gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt ggttgattca
     7081 tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt caaaatatcg
     7141 cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat tcttccttta
     7201 tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt taaaaattta
     7261 tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca ggattttctt
     7321 accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa tcactgcata
     7381 aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag cagtggaacg
     7441 atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca gtactactgt
     7501 catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc aaaaagcatt
     7561 tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc ctaaaagggt
     7621 agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag tcaatccttt
     7681 taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct tgctttgagc
     7741 aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa ataatacaag
     7801 cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga gcagcaaggc
     7861 taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga ttttggctgt
     7921 ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag gcaggaatgt
     7981 cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac ctcgatcata
     8041 gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca aagcattttt
     8101 tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa tcgaaaccag
     8161 tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga acaatggaaa
     8221 ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa ggatcccata
     8281 tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt ggtacaccgt
     8341 ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac gaacgaactg
     8401 ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga aattccctcg
     8461 ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga tcagcggttt
     8521 caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga caaacaacca
     8581 caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt gctttcgctc
     8641 gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac cggataaagc
     8701 taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa aaccttcgtc
     8761 gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg ctatgaaaga
     8821 agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat gatccaaatc
     8881 attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac aaacttttgg
     8941 atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg tatcaccatc
     9001 tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc ttcgaataac
     9061 tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat atgcgcctcc
     9121 tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg cccaattgtc
     9181 aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc atcttctagt
     9241 tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc gattttctgc
     9301 cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc gatctcaaca
     9361 gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc gcttagcatt
     9421 tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc acctaagaga
     9481 ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga tcccagttct
     9541 gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat cttctgctgc
     9601 tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc tcgcaattgg
     9661 tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt tgcgttcggg
     9721 atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg accttcaata
     9781 aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc ataaccgatc
     9841 cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt ttctacccga
     9901 ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc ttcgcgaata
     9961 gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc aggcggtgtt
    10021 tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct cggaatcggt
    10081 gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg ttccttatgt
    10141 tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct aaattcaata
    10201 tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt acgcagttgt
    10261 tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc gatatttacg
    10321 gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa gatcgttgtt
    10381 ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc tcgaagcgct
    10441 acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg tttttctttt
    10501 tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc agagtagggg
    10561 aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata tcctttttct
    10621 gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat ttttgaagag
    10681 acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg aggcgctttg
    10741 gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat aaacagtttg
    10801 tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac ttctagcgtt
    10861 tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc tcctgctttt
    10921 aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct cgtttgtttt
    10981 ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc ttgaggaagt
    11041 tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat caatgcattt
    11101 ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc tttgcgtatt
    11161 ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca acggtccatt
    11221 tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata ttgaaaatta
    11281 tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa aaaggttgtg
    11341 gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg ctcagaaaac
    11401 acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc gatttcccga
    11461 tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga agggaaataa
    11521 tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc ttcttctgtc
    11581 gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa aaactcgcgg
    11641 tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat ttgctctatt
    11701 ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac ccagatttct
    11761 tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga atctatctct
    11821 acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat atcgataatg
    11881 cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac atagcccatc
    11941 aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg ccaattcaat
    12001 tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc gactggcaac
    12061 aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct ctcgcctcgc
    12121 gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg aaataaatgg
    12181 atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc ataatacaaa
    12241 tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc aaatagaagt
    12301 gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc ttctaaattg
    12361 ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc cattcctttt
    12421 cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt ctatgagata
    12481 ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat tttctttgct
    12541 gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc cgattcccac
    12601 cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga taccgttatg
    12661 cccgccggca cttccctttt gacggagccg gatttttccc actgccagat ccaaatcatc
    12721 gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg ggccgactgc
    12781 acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt ctccattatg
    12841 aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt gatgctcgcg
    12901 tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt atttggtacc
    12961 tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc taaaaagcag
    13021 cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc ttttttacac
    13081 ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa tcgttaataa
    13141 atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat ataaaaatag
    13201 tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg ttatatttta
    13261 tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg aaaaaacaaa
    13321 tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt
    13381 tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag ttactcaaaa
    13441 tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag gagacgcaat
    13501 tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg ctacttacgc
    13561 agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa aaccaggtga
    13621 aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat
    13681 cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag atattttgac
    13741 ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt caggtacctc
    13801 tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg atgcacgtaa
    13861 cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt ggtcacatgc
    13921 aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga
    13981 agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa
    14041 aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg ccatcttaga
    14101 tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg gattgaacga
    14161 catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat
    14221 tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat taaaagaaat
    14281 tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga cggaaaacat
    14341 ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa ttttttcata
    14401 aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc gttccaccct
    14461 aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac
    14521 atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt tatacctatc
    14581 gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga ataaatgtca
    14641 gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct gataaaaaac
    14701 tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag ctcggctaca
    14761 aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga tggacttggg
    14821 gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt caaaaccacc
    14881 aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg aacaaagacc
    14941 ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg
    15001 aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa
    15061 actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa gtaacatctg
    15121 aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta
    15181 cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg agttggctga
    15241 cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat gtaacagatt
    15301 taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca aaaagaggag
    15361 aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt gagacagaag
    15421 cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt gtgcaaggtg
    15481 gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat ctggagaacc
    15541 agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc gtttccggaa
    15601 agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa gaagatgcaa
    15661 ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac tactggatgc
    15721 cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa tacggtggag
    15781 acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt gtaatgaagg
    15841 aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa aaataatata
    15901 taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc taacaatgtg
    15961 ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct tctagcttat
    16021 ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa
    16081 tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct cgtacagcag
    16141 caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc agatcttctg
    16201 cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt
    16261 caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt tctcccagtg
    16321 atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta cggttatctg
    16381 tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac gtgatttcat
    16441 cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc acattgtcgt
    16501 taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga ttcatagatg
    16561 gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc tgaaagactt
    16621 tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat ttactatgtc
    16681 ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta gtactatttc
    16741 acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca aaaagcacac
    16801 ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg aacataggtg
    16861 ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa
    16921 gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca cctaaaaaca
    16981 agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa aagaactcta
    17041 aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa ttaaaacgta
    17101 tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat cgcgaaactt
    17161 gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg actatcagtg
    17221 cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc ataagtcacc
    17281 tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata gagacttctt
    17341 ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt tttcatgagt
    17401 tattcattaa attggaatct agattctatt ttttccggag gcagtcattc tgatgctttg
    17461 aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa
    17521 tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg
    17581 attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc tgccaatgtc
    17641 aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc tcgtctgcaa
    17701 tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga ttggaatcag
    17761 atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat
    17821 ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc tttagatggt
    17881 ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat tcccttcgaa
    17941 caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat gatgggcgat
    18001 ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg gaaagaaaag
    18061 gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc tgactatgaa
    18121 cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt gaagaaagaa
    18181 acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt taattacttg
    18241 actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca agatgcacct
    18301 attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc agcatttatt
    18361 atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa
    18421 aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta ttgtactgag
    18481 ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt taatgaggtt
    18541 gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg ggatttacct
    18601 tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt cgcagaattg
    18661 atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta
    18721 gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg attcattttt
    18781 gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa gatcacagag
    18841 atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta ccatccatat
    18901 ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa cttcccatac
    18961 acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat
    19021 tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac ggaagatctt
    19081 gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc aggtatcgac
    19141 atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat ctgatttcta
    19201 ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
//
LOCUS       DANKHJ010000049        19236 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000049 DANKHJ010000000
VERSION     DANKHJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(66..695)
                     /gene="udk"
                     /locus_tag="PW113_001898"
     CDS             complement(66..695)
                     /gene="udk"
                     /locus_tag="PW113_001898"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2526194.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(779..1474)
                     /locus_tag="PW113_001899"
     CDS             complement(779..1474)
                     /locus_tag="PW113_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2526195.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1487..2203)
                     /locus_tag="PW113_001900"
     CDS             complement(1487..2203)
                     /locus_tag="PW113_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2526196.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2533..3141
                     /locus_tag="PW113_001901"
     CDS             2533..3141
                     /locus_tag="PW113_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2526197.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3466..3789
                     /locus_tag="PW113_001902"
     CDS             3466..3789
                     /locus_tag="PW113_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2526198.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            3971..5341
                     /locus_tag="PW113_001903"
     CDS             3971..5341
                     /locus_tag="PW113_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2526199.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5471..6772)
                     /locus_tag="PW113_001904"
     CDS             complement(5471..6772)
                     /locus_tag="PW113_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2526200.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7126..9084)
                     /locus_tag="PW113_001905"
     CDS             complement(7126..9084)
                     /locus_tag="PW113_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2526201.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9261..9527)
                     /locus_tag="PW113_001906"
     CDS             complement(9261..9527)
                     /locus_tag="PW113_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2526202.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10037..12163)
                     /gene="pnp"
                     /locus_tag="PW113_001907"
     CDS             complement(10037..12163)
                     /gene="pnp"
                     /locus_tag="PW113_001907"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2526203.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12351..12620)
                     /gene="rpsO"
                     /locus_tag="PW113_001908"
     CDS             complement(12351..12620)
                     /gene="rpsO"
                     /locus_tag="PW113_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2526204.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            12871..13434
                     /locus_tag="PW113_001909"
     CDS             12871..13434
                     /locus_tag="PW113_001909"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2526205.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13687..14208)
                     /locus_tag="PW113_001910"
     CDS             complement(13687..14208)
                     /locus_tag="PW113_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2526206.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14211..15053)
                     /locus_tag="PW113_001911"
     CDS             complement(14211..15053)
                     /locus_tag="PW113_001911"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2526207.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVNLMKHSREYQNK
                     MKEE"
     gene            complement(15046..15771)
                     /locus_tag="PW113_001912"
     CDS             complement(15046..15771)
                     /locus_tag="PW113_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2526208.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15947..16243
                     /locus_tag="PW113_001913"
     CDS             15947..16243
                     /locus_tag="PW113_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526209.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            16811..18403
                     /locus_tag="PW113_001914"
     CDS             16811..18403
                     /locus_tag="PW113_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2526210.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18416..19156
                     /locus_tag="PW113_001915"
     CDS             18416..19156
                     /locus_tag="PW113_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2526211.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5494 a         3947 c         3659 g         6136 t
ORIGIN      
        1 ctgttcaaaa agcagaatcc ttgcttttca aacggtcgca gtccgttcaa ctgtttcgct
       61 gcgcactatt tatggtttaa gaaactagct acttttgttg taatcaaatc aatagcaacg
      121 tggttttctc cgccttctgg aacaatgata tccgcatagc gcttcgttgg ttcgataaat
      181 tgatgataca tcggtttaac aactgtcaaa tattgttcga tcacggagtc caatgtgcgt
      241 ccccgttctt ccatatcccg tttgatacga cgaatgatac gaatgtcatc atctgtatct
      301 acgtatactt tgatatccat caagtcacgc agacgttcgt cttctaaaat caggattcct
      361 tctagaatga tcacttcttt tggttcctga atgatcgttg cctgacttct tgtatgcgct
      421 acatagtcat acacgggttt ttcaatggct ttatagttca gtaagtcgcc tacatgctga
      481 atcagcagat cattatcaaa ggcaaatggg tgatcgtaat ttgtgttcaa tcgttcttca
      541 aaactcaaat ggctctgatc cttgtaataa gaatcttgtt caagcatcat gatggaatgg
      601 tccgggaagt tgttgaaaat cgcacggctg acacttgtct ttccgcttcc ggatccaccc
      661 gtcacaccaa tgataattgg cttgcttttt gtcattgact aaaacctctt tcgtatccgt
      721 tttttcattc taatctatta tagcgaaaaa acacgaaaat gctatatttt tctgaaaatt
      781 aatccttttc aatttccaat aatgaataaa gttcctctaa tgaatcgatt cgataagtag
      841 gctggatttc ttcgatttct ggtaatttat ttctattgaa ccaaatcgta tcgatccctg
      901 ccatatttcc tcctttgata tcagaagtca aagaatcacc aatgatcaca gtttgctttt
      961 tatcaaaatt aggaatgtgg gaaaagacat gatcaaaaaa ttctttcatc ggcttttgcg
     1021 ctccgacagc ttctgagaca aagatatcct tgaagtaatc aagcattcct gcatctgtta
     1081 atcgccgatg ttgcgtgtgc gacacaccgt tcgtcacaat ataaagatca gcatgcggat
     1141 ggaccttttt caggatttct agacttcctg gaacaagatc atgaccttgg ttcaagtagt
     1201 ggcggtagcg tttttccatt tgatcactat ctactgtttt cccaaattgc tggaaaagta
     1261 acccaaaacg actgcctatt atttcttctc gtgatagtct gcctttttca aactcctgcc
     1321 ataaatgatg attcaaagcc ttatagctgg tttcgatttc tggtgtcaat gtgattcctt
     1381 gttctgcaaa caaagcactt aaagcattgg cttcagttaa ttgaaaatct aacaatgtat
     1441 catctacatc aaacaataaa gttttatatt tcatcttttt gacctcctat ttaaaataaa
     1501 tacttaactt tttcatctct gctaatgtca acgatcgata ctcgcctact cctagctgtt
     1561 catctaattc caatggtccc atgcttagcc gtttcaatgc ggtcactttc tttccttggg
     1621 ctaagaacat ctttttgact tgatggaatt ttccttcttg gatcgtcaat aaaaccgtac
     1681 tttggtgctt gtctgctttg agtattttta aatcagccgg cttacattga tacccatcag
     1741 cgaatacgat tccattttca aatgctgtta cgtctgccgg tgtcaccctt tcgttcacaa
     1801 ctacttgata agtctttgaa accttttttt ctggaagtag catttcatat cccaattgtc
     1861 cattcgaagt gatcagcaat agtcctgttg tatctctatc tagtcttccc actggatata
     1921 atggttggaa acgatcggca cttgcaatca gatcgattac tgtcgtcttt tctggatctg
     1981 atacagctgt aacagctttt tcaggtttat tgagaagata ataggactca tttgtttcta
     2041 agtgttcacc tgctacttcg atttgatgca gtaaactatc tacatttcga ttttgtcgaa
     2101 attcgactgt gccatctatc atcacttgct tcaaagcaaa cagccgcttc atttccttgc
     2161 gtgtcgtttc taatttttct tctattaatt tatctaaacg catgctgttt cctttcttat
     2221 tactactaat taattatacc ttgaatattc ttgcttgcaa atacatcatc atagttattc
     2281 cttgacttaa ttaacagttc acgttacggt taaatagtaa agtgaattgg atttctcata
     2341 cacagcgtaa tgacctggtg gagaaaagtt gaatcctgac tttctaaggg gagatgcgcg
     2401 catctctcct tttgcgcttt tttaagcgcc ctgccttcga tgatgtgcac gagattagcc
     2461 tttagaattc acttaaataa aatctgtccg ttaccaaagc agacagtcac attttaagga
     2521 ggaattttgg ttatgtcagt atcattagaa gtaagtaaaa gagaagtacg tccccgttca
     2581 ttaagaaaca aacttcgtca cgaagggaaa gttccagctg ttgtttatgg gtataaagta
     2641 gaaagtaccc ctatctcatt cgatggtttg acactaagca aattgttgcg tgaacatgga
     2701 gcgaacacag tcatcacgat ggacatcgat ggacaaaaag tcaatacatt ggttcacaag
     2761 atccaaacga acacttttac caatcatttc gaacatgcag aatttctatc tgtaaacatg
     2821 tcggaagaaa cagaagtcga aacagaagtc gttcttgttg gcgaatcagt gggtgctaaa
     2881 tctggtggag tgttagctca aaacttgtat acagtagtcg tatctgctac accagacaaa
     2941 ttgcctgaac gaattgaagt tgacgtgaca aacttggaat taggtgattc tattacagta
     3001 gccgatcttc caaaaaacga cgattacaaa ttcgtgaccg acagtgaaga gcaaatcgct
     3061 gctgtagtcg ctcaaagtga agaagaagaa agcacaggtg aaggtggcga accagaagtg
     3121 atcggttcaa cagaagaata atactgaaac aatcaggtgg tgttagaagc ggcgggtcag
     3181 cagcttttaa cacgcctttt ttatttctaa tcgtttattt tcatatgggg cttccacttt
     3241 tgtaaatggc tttacaaacg aaaaatccta atttaccaag cttttcctcg catactttta
     3301 tcgtaccttt ataaaaattt atactttgaa aaaaagtatt caattattgt tgcatttttc
     3361 ctgattcttt attagaatga accctcgttg aagtaagtat ttctagttta tcctaggatt
     3421 aattataaaa ataaaacatt cgttgattcg aaggaggtta aacagatgtt tgaaagtttt
     3481 gatagcagcc gcaaccgtta cgcttcagtt ggtgtcgtat caagtttgcc agatgaattg
     3541 attgacagta tatggtttat tattgaccta gatcttaagg gagtcattcc attagaaaat
     3601 atattaacct ttgatttgat cgaaaatcgt ggaaaagtga cgatgcattt ttctcaagca
     3661 ggaagcaatg tggaaatgga tattgatttg ccgtttgcct attcgaacca atttcctgct
     3721 caagtatacg cctatgatga cggtacgaga gaaacgatcc ttttaccaag tgagattaga
     3781 caatattaag gtgatagata aacgtggatg tcccttattt tacagaccag gccaagttgt
     3841 tgattctttg actcaattct tggcttcttt ttatggagtt tcataaatta tcattgattt
     3901 ctaattggta ttctcaggat aaacgctata tactactaac taaattttaa agatataagg
     3961 agtttaaaag atgcgcgatt taacaaaagg aagccccgct aaactgattt tgatgttcac
     4021 tgttccttta ctgatcggaa atgtttttca gcagttctac aatatgatcg atatgatcat
     4081 cgtgggtcag acgctaggta aaaatgcgct ggcagcggtt ggtgcaactg gaagcctgac
     4141 atttttgatc attggctttg cccaaggttt aacagctggt ttagccatta ttaccgcaca
     4201 acgttacggt gcaaaagatt atcggggtct gaagaaaagt tttgctgcta gtgttgtcat
     4261 tagtcttgtc gtcactgtga ttttaactgt cctaagtctc ctgtttattc gtccgatgct
     4321 tcaattgatg caaactcctc ctgaaatact tgatcaagca caaacattta tttcaattat
     4381 tttattaggg atctttgcat cagtcagttt caatttgctt tctaatgtca ttcgcgcact
     4441 tggtgacagc cgcacaccac tattctttct gatcattgcg gtcatcatca acgtcgtact
     4501 tgatttgatc tttattatct attttggcat gggtgtggaa ggtgcagcta ttgctacagt
     4561 aatcgcacaa gtttcttcca gcgtactgtg tctagtgtat atcaagaaaa aaatgccttt
     4621 gttacagtta cgcaaaaaag atttcaaatt tgaaaaagaa gagtttgctg tacacctaaa
     4681 tgctgctttg ccaatggcgt ttcaatcttc gatcatcgct gttggtgcta tcgtgttaca
     4741 agcagcacta aatagtctag ggacagatgt cgtagctgct caagccgctg ctggtcgtat
     4801 cgaccaattt gcaaatcagc cgatgatgtc atttggtatt gcaatggcaa ctttcactgc
     4861 acaaaactat ggtgcgaaag aatatggccg aatcttgcaa ggggtacgtc aaactttact
     4921 gatgagtgtc ggatttagtc tcttagcagg tgctatcgtc attttcttcg gtcatttctt
     4981 tgttcaattg tttgttagcc catcagaaac acgtgtattt gaattagcac aaacctactt
     5041 caatatcaat gggtctcttt actggatctt agcagtcctg ttcattttac gctacacact
     5101 tcaagggctt ggacaaagca agatccctac gatcgccggt atgatggaat tgcttatgcg
     5161 ttcttttgca gctatcgttc ttacaggaat gtggggatac ccaggagcag cagcagcttc
     5221 tcctttagct tggatcggtt ctgttgccgt gttgctttac tcttatatgc gttcgatgaa
     5281 acaattgaaa cacatgggtg aagaggaaca tttcacatta gaacatgcag aagcaagata
     5341 gccttgatta aaagaaatcg ttgcggctaa aaatgaaaac ttttattgaa aatatcttct
     5401 tcgtttaccg aaaacaggtt ttttagataa caaaaaggct gtgacaaaaa atgtcacagc
     5461 cttttttctt ttattctgtt tttctttccg tggactctac tttgaaataa tcgatatgtg
     5521 catgctggta ttctaatacc gtgaaacgga atgatgcaat ctcaatgatt gttcctgctt
     5581 ttatttcttt atatcggcta gaagcaaagc ccgcaagtgt agtaaaattc gtttcttcaa
     5641 actctggaat ctctacatga aaataacgct ggaaatcatc taatgttagt ttgccggaga
     5701 tgatataaga gccatctgac tgttccgcta tcaatgcttc gtccaaatct gtctcatctc
     5761 ggatttcccc gacgatttct tctaaaatat cttcaatcgt cactaagccg cttgtccctc
     5821 cgtattcatc ttttaagata gcaatatgct tacccttttt tcgcatgatc gtcaataatt
     5881 tttttatagg gatcgtttca gttgttacga tcggctcttc aatcaactgt tctactgtca
     5941 gctggtcatc tttataagat tgcttgatca aatctggcaa ggtgacataa cctaaaacat
     6001 tgtcttttga ttctaagata accggatagc gagtatgtcc ttgttcaatc gaagctttta
     6061 tcgcttcttt gacggtttct gaagcttcga atacttgcat ggacgtacgg tctacttgta
     6121 tatcttttgc caaggtatca tcaaattcaa acacgttttc caaataataa tattcttctt
     6181 tggtcaactc tcctttttca taagaattca atgcgactaa tgtcagctct tcttgagaca
     6241 atgtctcttc tccttcagat acgagacgca cacctaatag cttccctagc cctgcagctg
     6301 aatgattcaa tacccaaata aaaggaaaca ttactttata aaaataatga agtggtttaa
     6361 ctacgaacaa gacgaccttc tccgtattga cgatactata gctcttagga agcagttcgc
     6421 caataaccac atcgatatac gtaactaaaa taaatcccag cgccacagag attgtactgc
     6481 taatagccga actaaaaggt agtttaccga tcactggttc tagtaaagcc gcgacagatc
     6541 cttcaccgac ccacccgatg atcaaaccgg ttagcgtaat tcctaattga ctagctgata
     6601 aatagttatc taaatggtgt gtaacatgaa gagctagctt ggcgttcttt acattttcct
     6661 tttctagttg ttccaatctt gatcgtctga ttttcactaa agcaaattca cttgctacaa
     6721 aaaatgaagt aattgcaatc atcaaaaata aaataatgaa attgataacc atattctccc
     6781 gcctttctct ttttctattg tacccttttt ctacaaaaag attgaaaata atgcgtattc
     6841 atatttagga ataaaaaagg agatgcggca tttttcagcc acatctcctt atcacttcaa
     6901 aatcattgcg ttacttgttg ctaaacagct ttttcatgac ctctgataaa aacggtattc
     6961 aaaagcttgc atctacggca ttaagcccaa acagatgaaa aacatgagaa gctgttttta
     7021 tctgctcgtt cttactgctt gttgcagtac agctttttca tgacctctga taaacggtgt
     7081 tcaagtagct gacattcgac attagttcag gtcaaagcgc tactttcaca accgctcttt
     7141 caaccatgtt ttccgagtat ttccaatgat aagcggaaca gtttcatgtt ttacttcgct
     7201 gtactctagc ccaaaccatg tttctggcga ggtagcgatt tttttgattg ccagttttgc
     7261 ctgcatgatt tgacgttgcc attttcctag agttgtcaca ttggaaacta cttcatcaat
     7321 caggacaaat tggaaatcgc caacttctcg ccctggtgtc agagagtatc tttgtggctg
     7381 ttttggcaaa cggccttgtt tcatcaaatc atggatgact tggcgaacat atacgttgat
     7441 ttcctgttgg accctgaaac ctaagaacag attcacttgg acaataaaat cagtgtccat
     7501 catatctact tgatattctt tcgtatatgg ctcatccgtc acttccacat gaacaaacca
     7561 atagactcgt gctcttttag gccgtttatc taaaatcgaa tagatgaact gtcggccaac
     7621 tttatcttca gccatatcag gaaccaagaa gaccacattt gtctgataga ttggtaaaga
     7681 agtatcatca cgtaattctc taagctgcgg gatataatct ctcaaagaca catcctcggt
     7741 tgacgattcg cgaataatgt tcccttgttc ccaaataaac atcacgacta agatgatcaa
     7801 accaatcaga acggctacaa aaccgcctcg gaagaatttg acaacacttg agataaagaa
     7861 aacgacttct atcgcaccga agaaaaaggt aactaaatat gcaagccaac gagaatgccc
     7921 attttggata agataaaagt aaagcagtgc tgttgtcatc aacatcgtca cagtgatcga
     7981 tagaccgtaa gcagcttcca tatgatgaga cgttcggaat gtgacaacca ctagagaaca
     8041 ggcgatccat aaaatcagat tgactgctgg gatatacatt tgcccgatac tgttcccagg
     8101 atacatgatc tttaaccgag gcagcagttt caacttgatt gcttctgaaa ctagcgtaaa
     8161 cgaacctgta atcaatgctt gcgaagcaat gactgcagcg actgttgcaa aagcaacgcc
     8221 gatcacgatc caactttgag gcatcatttg gaagaatgga ttcaggttct tgatttgctg
     8281 tgcttcaggg ttttgataaa cattcaacaa ccacgcagcc tgcccaaaat aattgataac
     8341 caaacaaatt ttgacgtaag gccagctcag acgaatattt cgtttgccga catgtccaag
     8401 atcggaataa agcgcctctg cacctgtcgt agccaggaaa atatttccaa gaatgaacaa
     8461 gcctaatttg ttatctgcac taaaaagcag ctggatagca taataaggat tcagtgcacg
     8521 gataactgtc cagtcttgac tgaagttcat taatcccatt acgcctaaaa aagtaaacca
     8581 agcgaacatg attggtccga atgcttttcc gacaatatct gtaccaaagc gttggaccat
     8641 gaataaaaca aaaataataa ctaaagtgat aatgacgatc acgttctggt tattaccgaa
     8701 ccggtcaaaa aactgcggaa ttcctcgcaa gccttcaatc gcagtcgtta ccgtcacagc
     8761 aggggtcaat accccatctg ctagtaaggc agctcctccg atcattgcag gaatgatcaa
     8821 atatttccca ccttttcgga caagtgtgta gagtgagaaa attccacctt caccatggtt
     8881 atctgcattc aatgcaatca cgacatattt gattgttgtc agtagagtca atgtccaaaa
     8941 aatcaatgag actgatccga taacaaaatc aggattgatt cctgacaatc cgccattatc
     9001 ttcaacaatc gatttcataa cgtaaagcgg acttgtcccg atgtctccgt agacgactcc
     9061 catcgctaca agcaggccag ccattgatat ctttttggta ttgtgcgttg ttgccacgtt
     9121 tttccctcat ttcttgttat atggatatcc tttaggtaag cttgaatgat agatgatatt
     9181 cttatccatg acacccgctt tcctaaattg cttctaatta tccaagtaac acactgaagt
     9241 gtcaacttat ttgaataaaa ttattctttc tttggacgtt ctctgatacg tcttgccaca
     9301 aatgaataaa gagcaaggca aaaaattgca cccaataatg ctggtatcaa ataaaaaccg
     9361 ccgagttccg gtcccagtgg tcctagtaaa aattcgccga gccaactacc taaaaaaccg
     9421 ataatgatat ttccagatac gcctcccggg acttcctttc ctaaaattgc gccagctatc
     9481 aaacctaata taccgccgat aaataacgac cataaaaaat tcagcatctc ctttccctcc
     9541 tttatccata tttctcactt ttattttacg ctttattcag aaggactcca aaaatatgcg
     9601 ccaagaaact ccagaaaaaa agaagttgta acaaaagtgt tcagcttatt tccgtagaag
     9661 ctgcttctgg aacaccgtga atcagaggtt gtgaaatcag cggtttgacc tgagtattag
     9721 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa tatcgaggga
     9781 cagctgattg aacatcgttt attggagagg ttgtaaaaaa gctgttctgc atcaagtaat
     9841 aagaacagcc aagtaaaaac agctcctaat gtttttaact tggctggact tattaccgca
     9901 gatgcaagct tttgaacacc gtttattcga aaaaaaagga ctgtgacacg gcttttgtca
     9961 cagtcccctt ttttcgaata attattgtcc ccctgtcacc ttcacgatca caagtacatt
    10021 attcctatga attaaattat ttttcttcat ttttcgttga accatcttcg ttcaacattg
    10081 cttttcttga cagatttacg cgtccttgtt tatcgatttc agttactttc acaaggatct
    10141 catcacctag tttaacaaca tcttcgactt tattgacacg atcgtttgct aattgggaaa
    10201 tatggacaag tccatctttt cctttgatca agttgacgaa tgcaccgaat ttttcgatac
    10261 ggacaacttt ccctagataa acttcaccaa ctttgacttc tttggtcaaa tcttcgatga
    10321 tcttgatagc ttttttgatc atttctgcat cagctgaagc aatacttact tttccatctt
    10381 gatcgatatc gattttcaca ccagtttcat cgatgattcc gttgatcgta tcgccgcctt
    10441 taccgataac atctttgatt ttagcaggtt cgatctggat catttcgatt tttggtgcat
    10501 attggcttaa ttcttcacgt ggagcagcaa tcgttgaagt caattcttct aagatttcca
    10561 tacgtgcttg ttttgcttga gttaatgctt cagttaggat ttgttctgtg attccttgaa
    10621 tcttgatatc catctgtaaa gcagtgatac catcttttgt accggcaact ttaaagtcca
    10681 tatcgccaag atgatcttcc agcccttgga tatcagttag gatcgtatag ttttcaccat
    10741 cagaaacaag tcccatcgca ataccggcga ctggcgcttt gattggtaca cctgcatcca
    10801 tcaatgctaa tgtgccagca cagatacttg cttgggaaga agaaccattt gattccaata
    10861 cttctgctac taaacggatc gtataaggaa attcttcttc tgaagggatt acttgtgcta
    10921 acgcacgttc acctaatgcg ccatgtccga tttcccgacg ccctggagag cctgcacgtc
    10981 ctgtagaacc aacagaaaat tgagggaaat tgtagtggtg gatgaatcgt ttgctgactt
    11041 ccacccctaa accatcaatg atttggtgct ctcctagagg tgcgagtgta caagctgata
    11101 aagcttgtgt ttgtcctcgt gtgaacaaac ctgatccatg tacacgtggc aataagctga
    11161 cctctgatga caatggacgg atttcatcca atttacgccc gtcaggacgg atcttatcga
    11221 tggtgatcaa ttcacgtacg acatcttttt caagatcttc tgtgatttgt ttaacttctt
    11281 tcaacaagtg atcttcatct tcatgacctg caaatttctc agcataagct tcgcggatat
    11341 cgatcttcac ctgctcgata ttatcttcgc gagcaagttt ttcttcagtc attactgctg
    11401 ctttcattgt agcgtaagaa gcatcaaaga tttctttttt caatacttca tcaacttgca
    11461 gtaaagtcac ttccattttt tctttcccta ctgcttgaac gatttcttct tggaaagcaa
    11521 ctaactcttt gatagcgtca aaaccaaaca gcaatgcacc aagcatgtct tcttctgaaa
    11581 cttctttcgc gccgctttca accatattga tggcttgttt cgttccggca acagtcaatt
    11641 cgatatctgt ttgttctgct tgttctactg ttggattcaa gacgtactcg ccatttacac
    11701 gaccaacttc tacgcctgcg attgggccgt caaatgggat atctgagatt gatagtgcta
    11761 aagaagaacc tagcattgct gccatagctg gcgagcagtc tgtttctaca ctcatcacga
    11821 tgttcgttac ttggacttca ttacggaagc cttctgcaaa catcgggcga attggacggt
    11881 cgatcaaacg ggcagtcaat gttgcttccg tacttggacg gccttctcgt ttgatgaaac
    11941 ctccagggat tttccctgct gcatacattt tttcttcata attgatcgtt aatgggaaaa
    12001 agtctgtatc ttttgcttct ttagaggcca cagctgcact taagactact gtatctccat
    12061 aacggactaa aacggctccg tttgcttgtt ttgctagctg accgacttcg atttgtaagg
    12121 gacggccgcc ccatgtcgtt ttgaatactt gtttttctga cataacttct ccttttcctt
    12181 ctcgtgaaag caagacgcta ctaaacaaat gaaaactgcc gaaagccgtc ttttgagcag
    12241 ctttcatttg gttagtagga gtccatctag cttgatcacg gaattttact taattgatat
    12301 acgagtaaaa aaagtgagat tcatgtgaat cccactcttt ttgactaaaa ttaacgacgc
    12361 aagcctaaac gtttgatcaa ttcgcggtaa cgttgaacgt cagttttacg taagtaagct
    12421 aacaagttac ggcggtgacc aacttttttc attaggccgc ggtatgaatg atggtctttt
    12481 ttgtgcacgc gtgcatgttc attcaagtgg ttgatttctt cagttaatac agcgatttgt
    12541 acttctggag aaccagtatc cccttcatgg cgagcgtatt cgttgatgat ttcattcttg
    12601 cgttcttttg aaattgccat ttctttcacc tctttcgatt attcccctgc gactgagtaa
    12661 aacgttggtg attcgcttaa ccaagtggca ggtcatgtgc ctagagcaca cagctatact
    12721 ttacctgaaa ccgggttgaa aatcaagtag ttaatcatta agaaaaggta gttttcaaac
    12781 aatcttacta ctacttttcc atttttgtgg taaactataa tgcgaaaata gacttgaaac
    12841 acagtctttt aagaaagggg tacatattcc atgattacaa tggatgatat cattcgtgaa
    12901 ggaaatccga ccttacgcga agttgcaaaa gaagtttctc ttccattaag cgaagaagat
    12961 atctctttag gaaaagagat gcttgagttc ttaaaaaaca gccaagatcc aatcaaagct
    13021 gaagaactac acttacgagg cggtgttggc ttagctgctc ctcaattaga tatttctaaa
    13081 agaatcattg ctgttcacgt accaagtcct gacccagagg cagatggacc ttctctaagt
    13141 actgttatgt ataatcctaa aattttgagc cattctgttc aagatgcttg tttaggggaa
    13201 ggtgaaggct gtttatctgt tgatcgagaa gtaccaggat atgttgttcg ccatgccaaa
    13261 atcactgtgt cttattatga tatgaatggt gaaaaacaca agattcgttt gaagaattat
    13321 gaatcgatcg tcgttcaaca tgagatcgac catatcaacg gtgtgatgtt ctatgaccac
    13381 atcaacgatc aaaatccatt tgcactaaaa gaaggcgtat tggtgatcga gtagagaatt
    13441 ggcaggttcc ttcgatacta tgctacaaga aagctttata aaaaagggga gtgctcaaaa
    13501 gctgcacttc ccttttttat gtttctccac taatcgaaat ttagatagac atctagaaat
    13561 cgcaattcca aaaaattctg tgtattggat acgcatcttt tcttattttt tcttatttct
    13621 tccatatggg tgtttatctc agagatgata gaatagtttc taggataaaa agtagcagct
    13681 acgatgttat ccttcgtaga ggattcctgc ttcagctagt ttttgtttga tgtttttcag
    13741 catatgcttt tctgggtagc ggatcgtatg cagatggatg ccgcctgtta attcggataa
    13801 caaagtagca ttactttttt gataacgtct cataaaggaa tcgatatctt gtggcgtttt
    13861 gatgtgcaaa gttcctgcta actcaccgta aaggggatgt tccacaatta catcaatgat
    13921 ttcaccacca ttttcaacga ttagtctcag ttcttcttct gtttgatctt ttccatgctg
    13981 gacagcgatt ttactgatgt gcccagtatt ggtttcttct gtttcgagga tataacctct
    14041 tgctgttgcc acgatacttt tacctgcagc tcgtagtaaa gcaatatcgc ctacaatgat
    14101 ctgacggctg actccaaaag ttttcgcaaa cttgctagca ctcactggtt tttctgtatt
    14161 tgccaaggtt tgcatgattt gttttcgccg tgtctctcct tcgatcacac ttattcctcc
    14221 ttcattttgt tttggtattc gcgcgagtgt ttcatcaaat taacatacgt gtctgttatt
    14281 gtttccttat atcccttatt tttcagcaga ctaacaactt tatccaatac ttgctgctct
    14341 ctttttgcat cataaactgc ttggttttct tcttttttca cttgtcctat tttagttacc
    14401 gtatccattc ttttttctaa taaagcaacc aactctcgat cgatcatatc gatcttatga
    14461 cgtaatgttt caagagactc ttctgttagt tcttcctcta ctgtcttgat gatacgagca
    14521 ggatttcctg caatcactac attgtcggga aaagatttgg tcacaaccga acctgcgccg
    14581 actactacgt tgtcccctag agtgacaccc ggcgtgacga taactcctcc gcctaaccaa
    14641 acattattac cgattttaat gggttttgcg tactccagac cgctgttacg tttcactgga
    14701 tgtagtggat gcgtcgccgt gtaaagctgg acattcggag cgaacatgca gttatcaccg
    14761 atttcaatcg tgctgacatc taaaaatgta caattgaaat ttgcataaaa attctcccca
    14821 acaaatatat tatatccgta atcaaaattg ataactggct ccatatagat ttttttccct
    14881 gtacggccaa aagtctcttt caacaattgg gatcgcagtt ccgctgattc tgcttggttg
    14941 atgatttggc tttgacttct tgcaaacata cggtcggcaa ccagctcagg atcattggca
    15001 aaataaagct cgcctgcaat cattttttct ttttcggttt tgaattcagt cataatctgc
    15061 tcctaattct tgtatttttt tgaaaacaac cgctccgtct tcttgaataa acgtataagg
    15121 ctccgctaaa taaacaggcg tttgattgat tactaagatt ttggctgacg gctggcgaaa
    15181 ctgtaccaga tcacaaaacg gatgaacttg aaaactcgtt ccaacaatga cgatcagctc
    15241 tgcttggctg acggcataag ccgcctgctc catgactcca tcttgaaacc cttcaccata
    15301 taacacgatg tctgggcgta tttgtcctcc gcatgcttga tgtttgtcgc tgtgaagata
    15361 ttctttccag tctaccgttt gttgacattt tcgacaatga cattgataca gactcccatg
    15421 aaaattgacg agttgcgggc ttttagcctt tgcgtgcaaa ccgtcgatgt tttgggaaac
    15481 aacccaaaca tttttttctt tcgctagatg tgctatttcc aaatgaatga tattgggttt
    15541 tgcttcagga tgataaatct ttttgacaaa ttgatagaat ttatccggtt cattcaccat
    15601 tgcttgatga ctcagcaagt attccggctg ctccatcccg tggtagattc ctgacaaaga
    15661 tcgatagtct ggcactccag aaggtgtgga tacccctgca ccggtcaaaa atgtaatttg
    15721 ctgataagct aatatctctt ttgctgcttc gtctactgtt aattgtttca tgttttgtca
    15781 cctcactttc cattatagcc catactcgaa caaatttatt gttgaaaagt cagaaaaatg
    15841 ggaataaaaa agtgttatgg gcatttgagc aatttatcac aatgttcact gacaaaagaa
    15901 tgaaaacgtg ttacaatact gctgtataaa agtgaggtga atcgatatgt taccattctt
    15961 tatttattgg ggcgttattc tggcttgtat cgcctggtta gcgttaagcg tttatttctc
    16021 tgttttttat cttgtacgca aagaaaatgg aaatctttgg gcttttgctt tattcaacgt
    16081 gattgccgca attgttttag ccattacatt agccgtatat cgaacatggg gctggggaat
    16141 cacccaatac tcaagtttga tttatctaat attagccatt tatggcgtcg ttgttctttt
    16201 gcaagcgatc ctaggacgtg agccaaagaa agcggcagct taaatgatcg agatggaact
    16261 ggacacagga aattttctgt tctagttctt tttttattgt attacataca tttttgtgta
    16321 taattatact atcgtttgca atggatagat gattcacatt ttgattgttt tttcggtaaa
    16381 agtaaagatt tctatattta tcagtatttt ttcaaaaacc agcagttata gattgacaaa
    16441 gcctctcgag tatcgtaaac tttttttgta taaataactg aatacgagat gcctcaatca
    16501 atgaggtaga ggtcgcgaga atcattaacc ctgtggagtg aagcaaacat ggagaagcag
    16561 gaatagggag gatcgccgaa atgtgcactt ttttgcttgg agtgtatgtt gggacgcaag
    16621 ttaagagctt gcggactgtc ttagcagtga tgctaagttg cgctatgttt tgtgagaggt
    16681 ttatcacctt atggaatttt agcggccttt ttatatctat aaatcggtcg ctttttattt
    16741 tttcagataa agctttaccg catttcatct agaccgctcg tgtatcatat aatcagaaag
    16801 ggagaaaaaa atgaaaaaaa atatggtttc agttgccgta ctatttgctt ttgtcttctc
    16861 actattcagt gtgggatctc tggcaaatgc agatgaaaaa acaccgagcg atgagtttcg
    16921 cgtaggtatg gaggctgggt atgcaccctt caactggtct caacaaacag atgagaataa
    16981 tgccttgcca attcaaggcc aaaatagtta cgccggcggt tatgatgttc aaatcgctaa
    17041 aaaagtagca gatggtttag ggaaaaaatt agtcatcgtc caaacgaaat gggatggtct
    17101 ggctcctgcc cttcaatcag gcaagatcga cgcgatcatc gccggaatga gccctactgc
    17161 tgaacgacga aaagaaattg attttaccga tccttattac gaatcacaat tagtagtagt
    17221 agttcaaaaa gatagcaaat atgcaaaagc aaaaagttta gcagatcttt ctggagcaaa
    17281 gatcactgct cagttaaata cattccacta tactgtgatc gatcagatac cagatgtgca
    17341 gaaacaacaa gcaatggata atttctcagc aatgagaaca gctttagctt ctggaatgat
    17401 cgatggttat gtcagcgaac gtccggaagg tgtaacggca actagtgtga acaaagactt
    17461 ggaaatgctt gaattcagcg gtgcagatgg cttccagaca aacccggaag atgttcagat
    17521 ttctgttggt atgcgtaaag gcgacccgga tatccaaaaa gtaaaccaga ttctttctgg
    17581 catctctaac gatgaacgaa ccaaaattat ggaccaagca gtcaaagatc agcctgctgc
    17641 aactgatagt gaagaaaaac ctggcatcat ttctgatttc actacgattt ggaaacaata
    17701 cggcaatatg ttcttacgag gtgctggact taccttgttc atcgcattga tcgggacagt
    17761 tgtcggtact acacttggat tactgattgg tgtcttccgt tcggtacctg aatcaggaaa
    17821 tgcactgact cgcttcttcc aaaaaatcgg aaatgcactt ctttctatct atgtcgaagt
    17881 attccgcgga acaccgatga tggtacaagc aatggtcatc ttctacggtc tggcactcgc
    17941 ttttggcatt tcattgaacc gaacgattgc cgctttgttt attgtctctc tcaatactgg
    18001 agcttacatg acagagatcg ttcgtggggg gatttttgca gtagatgaag ggcagttcga
    18061 agctgcgcaa gccattggta tgacccatgg ccaaacgatg agaaaagttg ttattccaca
    18121 agttttacgg aatatcttgc cagcaacagg aaatgagttt gttatcaata tcaaagatac
    18181 agcagtttta agtgtaatcg gcgtagctga tttgttcttc caaggaaact ctgcttccgg
    18241 tgcgaatttc caattcttcc aaacatttac gatcgtcggg atcatctact tagtcatgac
    18301 ctttacgatc accagaatcc tgcgggcggt agaaaagaaa atcgatggtc cttcagctta
    18361 taccaagctt gaagaaatcg acaattcaaa tttgcaggaa taaggaggaa gaatcatgaa
    18421 tgaaaaaatt attgaaatca aacatctaag aaaaagcttc ggtgaaaatg aagtattgaa
    18481 agatatcagc gtaacagtca ataaaggtga agttgtgacg atcatcggtt cttccggttc
    18541 tggtaaatct actttcttgc gctgtatcaa tttactggaa aaaccaacag gcggcgagat
    18601 catttataat ggagaaaatg tattagctcc taaatataat ttacctaaat atcgaacgaa
    18661 tctaggtatg gttttccaat ccttcaacct gttcaacaat atgaatgtct tggaaaactg
    18721 tatgtctggt caaacaactg tcctgaaacg agataaagaa actgccaaaa aagtagcaat
    18781 cgaaaatctg gaaaaagttg gtatggatcg ttatatcgaa gccaaaccag cacaattatc
    18841 tggtggacag aaacagcgtg tcgcaattgc tcgggcatta tcaatgaatc cagatgtatt
    18901 gctattcgac gaacctactt cggcgttaga ccctgaaatg gttggcgaag tgcttaatac
    18961 gatcaaagac ttagcacata ctggcttgac aatgcttatc gtgacgcatg agatggaatt
    19021 tgcaaaagat gtatctgacc gtgtcatttt tatggataaa ggtgtgattg cagaagaagg
    19081 tacaccagaa gatatctttg tccatccgaa agaagaacgt acaaaagaat tcctttcacg
    19141 tattttaaat gcttaggatc aatagctgat tgggacaaag agctgcggtg tttaccgcag
    19201 ttctttttta tgaattactc ataaaaggta aatttc
//
LOCUS       DANKHJ010000050        19169 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000050 DANKHJ010000000
VERSION     DANKHJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(224..454)
                     /locus_tag="PW113_001916"
     CDS             complement(224..454)
                     /locus_tag="PW113_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526212.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(890..1456)
                     /locus_tag="PW113_001917"
     CDS             complement(890..1456)
                     /locus_tag="PW113_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2526213.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1456..2412)
                     /locus_tag="PW113_001918"
     CDS             complement(1456..2412)
                     /locus_tag="PW113_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2526214.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2412..3068)
                     /locus_tag="PW113_001919"
     CDS             complement(2412..3068)
                     /locus_tag="PW113_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2526215.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3438..5456
                     /locus_tag="PW113_001920"
     CDS             3438..5456
                     /locus_tag="PW113_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2526216.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5526..5732
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5883..8297
                     /locus_tag="PW113_001921"
     CDS             5883..8297
                     /locus_tag="PW113_001921"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2526217.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8645..10198
                     /locus_tag="PW113_001922"
     CDS             8645..10198
                     /locus_tag="PW113_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2526218.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10579..12363)
                     /locus_tag="PW113_001923"
     CDS             complement(10579..12363)
                     /locus_tag="PW113_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526219.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12378..14099)
                     /locus_tag="PW113_001924"
     CDS             complement(12378..14099)
                     /locus_tag="PW113_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526220.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14199..14960
                     /locus_tag="PW113_001925"
     CDS             14199..14960
                     /locus_tag="PW113_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2526221.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15927..16195
                     /locus_tag="PW113_001926"
                     /pseudo
     CDS             15927..16195
                     /locus_tag="PW113_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16867..>19169
                     /locus_tag="PW113_001927"
     CDS             16867..>19169
                     /locus_tag="PW113_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526222.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6307 a         3395 c         3831 g         5636 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctagtttt cgcagcagga
       61 attttgaaat agttcttatt agaatagtag agattgatta ataagctatc ttcgattgct
      121 aaaaagaaaa cttctgaatt ccatacttta taaaaatctt tgataaatct attcgaaggg
      181 tcaaatttaa accataattg tgtctcatta aaaagcataa ccattactcc gatctgtttt
      241 taaaattagt ttctacctct aatatatatc gagtttttat tttgccttca gagaatactg
      301 tttctttttt tgcagttaca ggttgtttat tttcggaaaa agctaatata gctaagattg
      361 aaacatccat ctggaattta tcttttttac tgctttgatc ataaaagtct gcatattcat
      421 cactgatatt ttttctaata aattcttcca tcataaaaat cacctcaaaa cgattatacg
      481 aacttacgtt cttgttgtaa agcgatattt gaggggcaaa aaaggggcat aatttgactg
      541 atattaagtt agctattgat atatgaatga catacaaaaa tataaaatct tttaaaaatg
      601 cttatatatc aatgtttttg ccattctttt atatatctgt tatatgtcaa aaaaatgtgt
      661 tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
      721 aagaacagcc aatcaaaaac agcttctcat gtttttgatt tggttggact tattaccgca
      781 gatataagct tttgagcacc gtgaataaag aggttatgaa atcaacgctc taacctgagt
      841 attagagaaa aaaacgcaaa acatatcatt tatgttttgc gttttttatt tatctttttt
      901 tctcaataca atataaaata ggcggctggt tcttttggtt gataaaccga taattcagca
      961 cattatatgt ttcctgtggc agtttggaac aaaaagaatc aaccatatcc agttcttttt
     1021 ctccaccttc atgaccatag tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt
     1081 tcaatatttc ttccatagct gtgcgtgtag tctctggcat agtgatgatt gctttatcac
     1141 tttttgggag ataccctaga ttgaaaatcc ctgctttgag attttgttgt tcagctatca
     1201 cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac acgttcttct aactgacgct
     1261 gttctagcct ttcacgagta gcatcgattg cttgttgctg gatatcaaaa ctatagacat
     1321 gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc gtggccattg cccatcgttg
     1381 catctacgac gtgatcccct ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca
     1441 aagcagtttg caacatcaga cgctcacctt tttccgtaca tcatattttc cttggaagct
     1501 atctctgcga agaagttctt catcaattgc attcaagact tcccattttt tcaagctcca
     1561 cattggtcca atcaaggaat cccacggcgc atcccctgtc aaacgatgga tgatgatctc
     1621 ttttggtatc atttccagct ggtcgcaaat gacattgaca tagtcaggac ggctcattaa
     1681 ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa
     1741 atggagcttg attccttgga tatctgaatc taaaatggtg cgtctgacat tttctttcat
     1801 catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc gtacaaacac ggatattatg
     1861 tttgcgtaat ttagccacgc catctaaata agtttgataa tcatgtgctc ggttgatcag
     1921 ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct acccataaat acatccgctc
     1981 gttcagttca gcaaggtaat caactacttc atctggcaga caatccggtc gtgtaccgac
     2041 tgataatcct acgacacctg gtaaattgac tacttgctca aatcgttctt tgattatttc
     2101 aactggcgca tgggtgtttg taaaattttg gaaataaacg atgtattgtt ggacatgagg
     2161 ccatttttga tgcatcatgt cgatttcttt ctggaattgg attggaagag ggtcttcagg
     2221 tgcaacgatc atgtctcccg aaccagatac actgcaaaaa gtacaaccgc catgggcaac
     2281 agtcccatct cgattcggac agtcaaatcc tccatcgatc ggcaccttga aaattttccc
     2341 gccaaactca tttcgtaaag cgtaattcca agaatgatat cttttatttc cttctgaata
     2401 aggaaaagtc attaaaagtc cttctttctg taagagttat ctatttgttt tcttttttct
     2461 ctatacactg gataagtcag gcacagccaa gaaagactga gtaagtaacc gccgatgata
     2521 tcagtaggaa aatgaacgcc cagataaatg cggcttatcc cgacacttac tataataaac
     2581 atcatcagca attgaagcaa tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga
     2641 aatagtaaag aaccgtaaag aatcatactg cctgctgcat gtccactagg gaaactgtaa
     2701 cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc
     2761 accgggttga tgattccagc aattacaata aatccagaaa cgaaccaaaa tgtttctgcc
     2821 ctttttttct tataccacat ccataaagcc gcacaagcaa ataaaatgat gacggtaaga
     2881 ggattaccga atttagtcat aaagagttgg tagctgttca gatcaggata agaagctctg
     2941 acaatgcctg taaaccactg atcgaaacca gaaagccacg acagatggaa ctttacgatc
     3001 cagcccaaaa tgaaaaacag gacaagaaag aagctaccag ccacctgaaa tgatgtttta
     3061 tatttcatac aattcctcct ttgaagcaat gacttattat agcatttttg gtccagcgaa
     3121 aaaaacggta aagagataaa accaatgtat gattcctctt tgaataacca atattcttat
     3181 aaaaaaaata actgtaatcg gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa
     3241 ataaaatcag tgtttttcga aatcctttta aacaagaacg tagacgtagg aagaacttga
     3301 aaatgaacag aagttgttca ttacggtaaa ttacaatatg attacaaaaa acaacaaagc
     3361 tcttaaataa cttttaataa atattaagag aagttataat tattaataat agataaattg
     3421 aagttggagg atagaagatg gaaaacattg caagaaaaga acggagacgt ttagaacaga
     3481 ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg atggtaggct
     3541 gttctgtcgc tgccccatta gtacagccgg ttcctgttca agcggatgaa acacctgttc
     3601 agttcggcgc aagaatcaat acagcggcat ttatcacaga aattgctact tatgctcaac
     3661 caatcgctca agccaacgat ctatatgcat cagtgatgat cgcacaagca gttgtagaaa
     3721 gcggttgggg cagtagtgcg ttatctcaag caccttatta taatcttttt gggataaaag
     3781 gcagctatca aggacaaacc gtctacatgg atacgcttga atttttaaat aatcaatggg
     3841 taaataaaaa ggagcctttc cgacaatatc cttcttttgc tgaatctttc aatgacaatg
     3901 cctatgtact tagaaatact tcatttggca acggttatta ctatgccggt acttggaaga
     3961 gcaacacgaa atcctataca gatgcgacag cttgtttgac agggcgttat gcgactgatc
     4021 ctggttacgc aggcaaatta aataatatca tcgcaactta cggattgacc caatatgata
     4081 caccagctag cggaaacgca ggcggtggag cgactacagg tggcaataat actggaagtg
     4141 caggtagtac tggcggttca agcaattctg gtaatacaaa ctcaggttca gcaacttcga
     4201 ccacttatac tgtccaatct ggtgattctg tttggggaat ctcaaataaa ttcggtatca
     4261 caatggatca attgatccaa tggaacaaca tccaaaacaa tttcatttat ccaggacaaa
     4321 aactgacaat caaaggtagc caggaaaatg gctcttcgac gaataattcc ggtaacaata
     4381 ccaattcttc aggaaatgct ggtacatcaa acggaggaca aacaactggt gcgaaataca
     4441 ctgttcaatc tggtgattct gtttggaaga tctcaaatga tcacggaatt acgatggctc
     4501 aattgattga atggaacaat atcaagaaca atttcgttta tccaggacaa cagctaatcg
     4561 tatcaaatgg cggttcttct aatgctggag caaatactgg ctcaaatact gggaatacaa
     4621 gtggctctag ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg aaatatacag
     4681 ttaaagccgg agactctgtt tggagcgtct caaataaata cggaattacg atggctcagc
     4741 taatccaatg gaacaatatc caaaataact ttatctaccc aggacaacaa ctgactgttt
     4801 caaacagtgg atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc aatacaaata
     4861 acagcagttc agttaatact ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag
     4921 gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac gatggatcag ttaatcgaat
     4981 ggaataatat caaaaacaac ttcatttatc caggacagca attgatcgtt aaaggtggaa
     5041 cttcggcaaa tacaaataca ggttcaacta cttctgcagc gaaaccaaat acaccgaata
     5101 cgtctgctac gacaagtaca tcatccactg gtaatacgat gtataccgtg aaagcgggcg
     5161 agtcagtttg gagcgtagcg aacaaacatc atatcacgat ggatcagtta atcgaatgga
     5221 ataatatcaa aaataatttc atctatccag gacagcaagt gattgtgaag aaaggttcat
     5281 ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac ctatactgtg aaatctggag
     5341 aatctgtttg gagtgttgct gacagtcatg gaattacaat ggctcaacta atcgaatgga
     5401 acaatattaa gaacaacttt atttatccag gtcaaacgtt gattgtagct aaataaaata
     5461 gctgttcgaa gaaataacga ttgaattcca caaaaatatt tgttactata aggctactta
     5521 aatatgacaa cacttgaaaa ggaagtagta aaaagactga agtttcagag agcgcatggc
     5581 tggtgagaat gtgcacggat gcttttgaac tcgcctttga gttgcccttg ctgaataccc
     5641 gagtaagcaa gcggcggtga cggccgttac aacgttcgag gccttttcac taaggtaaaa
     5701 ttaggtggta ccgcgtagat tacgtcctat aaaaagagca atctttttat aggatttttt
     5761 tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa acagattctg
     5821 ccggtttttt cgttatattt ataatagaaa caagtgtttt caatagaaca ggaggacagc
     5881 aaatgagtta caatcataaa gaaattgaga aaaaatggca aaaatactgg gcaaaaacga
     5941 atactttcaa cacccatgat gatcctgaaa agccaaaatt ttacgcactg gatatgtttc
     6001 cctatccatc cggtcaaggg ctacatgtag ggcatcctga aggttatacg gcaacggata
     6061 tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg gggtgggatg
     6121 cattcggtct gcctgctgaa caatatgcgc tagatacagg aaacgatcca gctgagttta
     6181 cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc tttaggattt agttatgact
     6241 ggaatcgcga agtgaataca actgatccgg agtattataa atggacacaa tggatcttta
     6301 cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac tgggtacctg
     6361 aattaggaac agttatttca aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct
     6421 atgatgtagt cagaaaacca atgcgccaat ggatgctgaa aatcactgct tatgctgatc
     6481 gcttattgga tgatctagaa ttagttgatt ggccagaaag cattaaagaa atgcaaagaa
     6541 attggatcgg tcgttcagta ggagcaaata ttgaatttaa agtggctggt acggataaat
     6601 cctataccgt atttacgaca cgaccagata ctctattcgg tgcaacttac tcggttcttg
     6661 cacctgaact agatcttgtt agagaaatta cgacccctga acaaaaagct gctgtagaag
     6721 cttatatcga agagacagct aaaaaatcag atctaaagag aacggatctt gcaaaagaaa
     6781 aaacaggagt atttactggt gcttatgcaa tcaatccagt taatggcaag gaaatcccaa
     6841 tctggatcgc cgattatgta ttatcttcat acggcacagg agccatcatg gctgttccag
     6901 cacatgatga acgtgacttt gaattcgctc aaactttcgg actagaaatt cttccagtta
     6961 tcgaaggtgg agatgttgaa aaagctgctt acacagaaga tggtacgcac atcaactctg
     7021 aatttctaga cggcatgaac aaacaagaag caatcgataa aatgaatgaa tggctagaag
     7081 aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg tgactggcta ttctcccgtc
     7141 aacgttactg gggtgaacca atcccaatca tccattggga ggacggaaca gtcacagctg
     7201 tcccagaaga agagctacca ttacggttgc ctaagacaaa aaacatcaag ccaagcggta
     7261 ctggagaatc tccattagca aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag
     7321 gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc tcatggtatt
     7381 acctgcgtta tatcgaccct cataacaaaa aagaactagc agattatgaa aagttgaagc
     7441 gttggctacc agttgacatc tacattggtg gagcagaaca tgcggtactt catttactat
     7501 atgcacgatt ctggcataaa ttcttatacg atatcggtgt tgttcctaca aaagaaccat
     7561 tccaaaaact atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat
     7621 ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca gatacactgc
     7681 gcatgtacga aatgttcatg gggccattag atgcttcgat tgcttggagc gaaaatggct
     7741 tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat gagaacaata
     7801 aaatgcgtga ccgtatcact acattgaatg atggcaagct tgataaagtg tatcatcaaa
     7861 cagtgaaaaa agtgacagaa gactatgaaa atcttcattt caatacagca atctcccaat
     7921 tgatggtctt tatcaatgaa gcctataaag tggacgctct gccttatgaa tatatcgaag
     7981 gatttgttca actactcgca ccgattgctc ctcacatggg tgaagaacta tgggcaatcc
     8041 ttggaaatga tggtggtatt tcttacgcac catggccaac atacgatgaa gcagcacttg
     8101 ttgaagacga agtagaagtt gttttccaag taaacggtaa agttcgtgcg aaaagcaatg
     8161 ttccccgaga cttaggaaaa gatgagctag aaaaagcagc attagccaat gaaatagtgc
     8221 aagaatatat tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac aagttagtca
     8281 atatcgttgc taattaattg gctctttgtc aataaggact gatgagttgt gcaaaatcaa
     8341 atctgggcca gaatgaactt cattctgtct cagatttttt gttatttcaa attgattaat
     8401 tgattgatca aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt ctttttcttc
     8461 tgaatggtct tgtaagagat ctctgtaatc atcaaatttt tgtctattaa tcttagtatt
     8521 ttttgatgta taaactacct gaaattgaga gtacatacca aattcataaa ttttttatta
     8581 gtgggactag tgattgtatg gattttgacg cataatgaat gaagtatgag aaaaggagag
     8641 acaaatggat caagaactat ttattatcga agtattatca gctaaacaaa aaattatcga
     8701 gaaagcgaaa aaaagagcga aaagaataat gttcaagctt ttaagcttag tatttttctc
     8761 tggtttgctt tttttcggag ctgtatcgtt cctatatcgc catatgagtg cagtagtccg
     8821 ttcagctgaa gggatgcaaa aattaaataa tttgtatcac ttttcgtggg gagttggcgg
     8881 aataacaatt gttgtgctta ttgtttttct tctacttatt tggctccgtt accatcaatt
     8941 gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa cagtttttac ataataaacc
     9001 aacttctcaa gatgatgact atctttattt tgagaagact tcgacgagtc aagcttttcg
     9061 gatacaaaaa agtgattgcc agttattgct gagatcactt gatgatttac ctatctactt
     9121 aggcgaaagc aaatcaaaat tcggaatgaa cacattccat atttttttac tagaaaacga
     9181 acccagatgg gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta agatgagtac
     9241 attagtcact ggttttttcc tcattctttt agctggaaca atctattatg ggagatacac
     9301 tcagccaaaa atgtcttctc atataaatgc tgcttctcac atgacaatga actcaactaa
     9361 cgatacgcat atacaaaaga caaatgaagg gttgttagct gatcctaagc aaaaggtaaa
     9421 tttgacaaca caaaagataa atgatctcaa acttgataca gagacgcact ctctctatat
     9481 gacaacagat agtggtgcta attgggtatt tgtgccgatt gattcgcaat ggttgcgttt
     9541 tggcgaatac accctgacaa cggggctgat tcctgaaggt tattggatgg acaacacata
     9601 tgatatttcc gccgatttct catggtttat ctattcttct ggggatgatg tacgtctgct
     9661 gacttcaaga gataatggta aaacatggca aacgtctatt atcacccaat cagttcagcc
     9721 gatacgttat cgaaaagcgg tattttatgg caacgatggt ctatttatct gtacatcagc
     9781 cagtgggatg tctgcagaaa ctttgtacat ttacactact atggatggcg gaatcacttg
     9841 gataaaaagt ggaagtacaa tgatcaatca gccagtacaa aatgtcagct tcgttacaac
     9901 ttctttaggt tttgtttcga cacgtgaaag tttgttttat acaaacaacg gaggacgctc
     9961 attcaaagaa tcagttctgt cgattccgga aaactatcag acagggggga tagatctttt
    10021 tcaatcacca aatgaagtta ctcaaataag cgcgaatacg ttagagacaa aattttattt
    10081 gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc gttcaactga
    10141 taacggggaa acatggcaat tcgttcagca actatcgcaa gtagaacaag gagattaaag
    10201 aagagagatt gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag
    10261 cttctcatat ttgttgaata gtgtaactcg tgattcggag ttgacaagag caacaagaag
    10321 cgattttgaa gtatagcttc tcgtatttta caagatcgtg tcttattgcc gataggttgt
    10381 ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc actacttacc
    10441 gaacgaaact gaaggagcta tccaaaaagt atcattagta ttattctttg tgtttctagt
    10501 ggcaccacta gtacatttaa aaaagctatc gatcatggac taatcataca tgatcgatag
    10561 ctttttagat aatgtttgtt acgctatctg aaattgacta ttataaagac tggcataaag
    10621 cgttggtgat tggagtagtt cagaatgtgt tcctttttcg acgatatcgc cattcttcat
    10681 tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt
    10741 cctatctttt gtcacagctt ccattgcttg ctgaatcaat gcttctgttc gtgtatctac
    10801 gctagaagtt gcttcatcta aaataacgac aggaggattc gctagaatga ctcgagcaat
    10861 tgtcaataat tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc
    10921 atatccgtct ggcagtgttc gaataaagtg atcacattgt gcgatttcag ccgcttcgat
    10981 aatttgttct cttgtggcat ccattttccc gtaggcgata ttatcagcta ccgttccttc
    11041 aaacagccag gtattttgta aaaccatccc aaaagaacga cgtagttctc cacgtgaaag
    11101 cttggtaata tcaagaccgt cgaattcgat ctttccttta ttcagttcat aaaaacgcat
    11161 cagcagattg actaaagtcg tttttcctgc accagttgga ccaacgatcg ctaccgtttg
    11221 ttttggcttt actgaaaaat cgacttgatt catcaatatt ttatctgatg tgtaaccaaa
    11281 ctgtacattt ttgaaagcta tttttccttt cggttgttct aaaagtcttg catctggaag
    11341 gtctggaatt tcatctgttt catctaatat ctcaaatatc ctatcgattg ctgccattgc
    11401 ggattggatc gagttgatca cataagaagc ggtagaaatt ggttcagaaa tctgattgat
    11461 gtactgaaga taagcttgta agaagccgat cgtcacacca cctgatagaa caagcatagc
    11521 accgaatacc gcacttatga tgaaggcgag ttgattaatg aaacgaatgg ccggatagat
    11581 agcacagttc ataaattgag cacgtttaaa agcgttgtat tgctttttat ttacttcgtg
    11641 tatagcagct tctgcatgag cttgctgatt gaatgttttg accactagat taccagctaa
    11701 gatttcttcc attcgattat ttaaaacacc cagttcatac tgattctgtt cagaaatctg
    11761 tttgtttttg ttcgcaatct tagttgtaac aattgaactg ccaacgatca gcaataagac
    11821 gagcaaagta agcttgcttt cgatataaaa cagcatcaga ccagcagcag ctattgttac
    11881 gacagaagta aaaaattgat tgatacctgt caataacact tgagaaagtt gattgagacc
    11941 ggtagttgtc cgactaatga tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa
    12001 tgttttgaat ttttttgtga tttcttttcg caccctcaat gtgatctttt cgcttaacgc
    12061 tgccattgtc cgttcctgaa taaatgaagt gatgctgctt aagactgaac agccgatcag
    12121 caataagaca gggaacaaca atgcttgttt gatatcactg aaagaaagct gtccaatacc
    12181 tgtttttctt atttctgata agagatcatc gatcccgatt cccatgatca agggcatggc
    12241 tacgactaat aggttcccga ataaactgca tagaaacaat ccgtaaaaat atttatgttc
    12301 aggtttgatc attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc
    12361 catcgtatcc cttctttcta tattccttgt gattttgcaa actcctgata agtttggcaa
    12421 ttagacagta gttcttcatg agtaccttgt ccaacgattt ttccttcatc taaaacaatg
    12481 atgttatctg cttgttggat tgtactgagg cgttgagcaa caatcacgag agttttctcc
    12541 gacatgtgtt tatgaagggc tgctcttaat gctgcatctg ttttatagtc taaagcggag
    12601 aaactatcat cgaatacata gatctctgct ggtttgatca acgctcgagc aatacatatc
    12661 cgctgtttct ggccccctga ataattagaa ccgccttgag caacaaagct gtccaatccg
    12721 tcaggaaggg tgtccactag ttcttctaat tgagcgatct tgatcgcttt agacaactct
    12781 tctgttgagg catattcatt tcccatctgc aagttgctta aaattgtccc gctgaataag
    12841 aaagccttct gagggacata actgatgtgg gagcggatct gttcttgagt catttgagta
    12901 agaggaatac caccaagggt tacttttcct atcgtagggt cactaagtct caagagaagt
    12961 ttcgccaaac tactttttcc agatccggtc ccgccaacga tcgctgttgt ttttccttta
    13021 ggtattgtga aatcaatatc ttctagaact ggatcagaga tttcagtata tttaaatgtg
    13081 acatgttcaa aagcagctgc atttgtctga tcaaaggttg gttcagctac tgttttgatc
    13141 gtctcttcct caatttcgat ctctgcttgc agtatttctt ctatccgtcg taatgaggca
    13201 aacgatttag ggataatgac caataccata gctgcgatca tcaagtaagc aagtgtcagc
    13261 atagagtatt cgactacagc agtcacgaca ccgatttgta atgctccttc tgctacaaga
    13321 tttccgccaa accacaaaat agaagcataa acgattccca tgagcaaaaa agcaattgga
    13381 gtgacccaag caaatattcg gttcactttg atcatttgat tagcatagtc agcaaagctc
    13441 ttattcgttc gcttttcttc atatgattga ttatcaaagg cacggatcat attaatccca
    13501 gtaaagaatt ctctcaatgt gatggtgatc cgatccattt ttggctggat actcaaagat
    13561 aaaggtgccc ctcgtttcat cagtcgataa acaatccata cataaaaaac aacagagatc
    13621 aatgtaaccc atgttagtaa tagagaataa cgaaacatca tgagaatggt gaaaacgcaa
    13681 ataaccggtg ctggtaagat catttggcaa aataaaacaa tcatttgctg gacattatcg
    13741 acatcattcg tcattcttgt caaaagagat ccagaactaa aacgactgat atccttcatt
    13801 gacagctgtt ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa
    13861 gccaatgctg aaaagtaact agaggcaact gcagcgattg ttcctagaaa cgctacaagc
    13921 agcatttcaa ctccaatttt ttttataact gaggcttcgc cacttgctat acctgtgtcg
    13981 atcagctttg ctactaattt tggtacacct aatgtaccgg cgatttctaa acagatcatc
    14041 acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga atgtttttaa gatagacata
    14101 ttattcctcc ttttttcctg atacttttcg attctatacc ttcaagtaac ttgaaggtca
    14161 atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg
    14221 gccaagtatc ggaactattt gatttgccaa aatcaacgat ccgctattgg gatgaacaag
    14281 gattaatccg ttcaagtaga caagaagaga atgattatcg attgtttgat atcgatgata
    14341 tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc aaacagatga
    14401 agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa acagaaagaa
    14461 gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa
    14521 aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa gagatccctt
    14581 ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa tttctaccta
    14641 actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg atgtatggat
    14701 tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg gagaaaaaag
    14761 atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg gaacgtaata
    14821 atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg
    14881 gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat tatcatgcat
    14941 ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg atggttgttt
    15001 tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga ttattagtcg
    15061 tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaatgg aatggttgca
    15121 attcttagcg atatatacga tgtaattgtc ctcttaaaaa agtatggaaa aataattgta
    15181 ttgggtgtta tgtataattc atggtgaact gagactaatg agaagaaaaa ttcatattgt
    15241 tatgagtgag attgtatcag atgagaatga taaattaaat aggtgcataa aagcattaca
    15301 cttaaaatac attgttgctt acattattac aatatgttaa taaggcaaaa ataaatagat
    15361 ggattgaata aacatatttt ttaatcaaat agtccctact gtgactttcc tcagctatcg
    15421 gtgatactac cgatagcatt tttttgctta taaaataatt ataagtattt agagattttt
    15481 cttctaacat tcttcaaaca agaaaaatca caaaggttat tgttattttt atatattctt
    15541 tttaagaatg attgtttttt tatagaaatt ttaaaaaata aaacacgtta ttgatggata
    15601 gttagtttgt tatttggtaa tattatagaa gttgactggt agtgatttat ttttcgtcgt
    15661 tttataaaaa tgttattttt tggaaaaatt cacaaatatg taacgaatgt ttttctggaa
    15721 tttgctgtgt taaaaagcag tatgactaat ataaatacta ttgtcattta tgcaattttt
    15781 tatggatagt aattttagag gggaatcaat gaaggaagaa tagaagggaa aaatagttgc
    15841 aaatacgcca gttagcaaaa atagcaatac atcttcaagc tttaaaattc gagagaatca
    15901 tcttggtagt ggaatcaact acaacaaaag gattaaacca attaatagat agaattacca
    15961 atggaaaaat agaaaaggta gttattctat acaaaagttg tttaattcgc ttcggttatg
    16021 aacttagtga aaccctttgc aatatatggt acagaatata gaaattattg acattacaga
    16081 aaatgatgaa gatttaatac agattgtcac tatttgtaga taccttttac aaggaaaccg
    16141 agccaataaa gctaagaaga tgattaagga gttgatggaa gacgatacaa gttaagaaag
    16201 tgaaactacg cccaaataaa gagcaaaaag agtaattgga cgtggaaaat gcaagaaatg
    16261 aaccatcgat tcggtggaca gtttattagt aatgacggtt tgagaacatc catgacgaga
    16321 atgaaggaac atcctaaatg cgtttagttg aaccaagtgc caaataatat ggccaaataa
    16381 gcgatcagtt atcgatgaat cccaagtatt ctaaactata tattcatttt gatgctaagc
    16441 attgatatat ttttttggga ttgatgaagg ttaaccaata cagggactca ctgatgaaat
    16501 tctagagtct gaccttggta tcaacgattt agtgattgtt tctaatggaa aagtataaaa
    16561 gtatataagt acattaacaa aataactatt agggttacgc tccagcttgt gcaaggcact
    16621 ggcaggagga atcgtgaaat cataattatg gttaagccac acggacaact aaaagtcata
    16681 caaagctatc gaatcaatca acagaagaca atcggcaatc attagctttg tttacagctt
    16741 acattgcaac gcttgagttg aaccgaaaat acgagggagg taacgcagcc attttgagtt
    16801 ggggaatctt tttagaaaat tatcgggtac cttgttgttt agtcaaaatt ataaggggga
    16861 gtaatagtga ggaacaaatt tcataaactg ttaagtatgt ttctagttat gcttattgtg
    16921 atcaatcaat ttgggaacat aacggttcta gctgagtctt tagcagaaca gccagagcgt
    16981 tcgctgacga tcgaagttga tcaagaggag gctgctttgg gtgagcagtt ccagttgcga
    17041 gtcagaggaa caccagacct gctaaatgca ttggtcattg ataagagtga ggatttgatt
    17101 gaaggggaag tgaagcaact caatgattac gaacgtctga ttacttattc ttcccagcat
    17161 tcaggtaaat acgaattgac cgcaagaagc ggtgatattc aatcaaatac ggtctcaata
    17221 gaaatcagtg taccagaaga aagcacaagt gagtctttta atgtaccaga agaaagcaca
    17281 agtgaatctt ttaatatacc agaagaaagc ttaagtgaat cttttaatga agccagatca
    17341 tctgagccat ctataacgca ggaatctcag attgaaagac aagaggagct cttgaattat
    17401 gacatcggca tctctgctga gcgccccact ttgccgagca ggcatacaca aacgttaaag
    17461 ggatacattc aaggaggcgc tggcgatatt cttgaagaat atggggagat ggtcgtacga
    17521 gttcctagag catcgattgg cttaattgag ttcattagag tggaggtcgc tgatgattca
    17581 cctttaaagc cactggcatt ttaccaagaa ggcacagacg ttgtttttcc atttaccttt
    17641 gattatgaag gattaaatga agccgctcag tcgtatacat tcagtatcac gtttgaagtt
    17701 gctttctttc aatcaggcgg tactgagcca aataatgact ttcaagtgag tcttacttat
    17761 gctgagcgag cagcaaacac ggaattttca attacgccta ttccttcatc aagacccatt
    17821 ttttcgaaat tatactatgg ctctagagaa atcgacagcc aaggaaatct tgggaatctg
    17881 ctgattgacg ggcaagacgt gaccaacagt aataaattta ttttgcaggt aaattatgaa
    17941 ggatcacgaa aagacggtgt agtggttacc gatgtactac cgcaaggaac aactttggca
    18001 ccgaatccga ctacatggag ttccactgga aatagagaac ctatttcagg tggggttcgg
    18061 atcgcagaag taacgaatcc aggtgaagca aacgttagaa ctcattatgt ttccgaggaa
    18121 tttgtggaca aagtatcttt taataaggaa acgaatacac ttgttgtgga ctttggtaat
    18181 ctagcagagg gtcaatcgta ttttattgaa tatgctcttg aagtgaccga tgtcgacttg
    18241 ttcaatgaag gaagacaaga ggatgggttt tctcggaatc gagcgagtat gacctacaat
    18301 aatcaggtaa tgagccgaga ctaccgcttg agaatccctg aaagtaatct tccggcaatc
    18361 tctttaagaa aaagtgtgga caaaaatgtt atcaatctca atgaaaatga actaacgtat
    18421 actctgcatg tctcgatgtt ttctggaaca agaaatacgc ttgttattga agatgttttg
    18481 gaagaaggca tggaatttgt gtcatttcta tcagatattc cctctttcat tgatgccgaa
    18541 gtcaataatg gaatagttcg tttcagtgcg aataatacga ttggtggtcc gacaagtttt
    18601 accctctctt ttaaggtcaa tgtagaggcg tatgcggtcg gcgatcaaat tttaaacttt
    18661 gcaacgcttg tgaatagtga caatcgattg aatacgagta ctgtaagaac aaggaaaatc
    18721 gatgggcgta ttaaagtaat taaaactgat gagaatggcc aaaggctggc aggagcaaaa
    18781 tttgaaattt tatcaagcga ttcacaagtg atacaagaag gtataacggc agaaaacgga
    18841 gaatttttaa gtgaaccgct tcctgtaggt agctatcaag tccgagaaac agaagcaccg
    18901 gaagggtatc ttttagatgc gacggtacat gatgttttta taaatgaata ctccatagaa
    18961 gaagtgacgt taaccattga aaacatcctt gatacgggtg gtgttgaact aattaaacat
    19021 gctgaaggca gtggtgaagt ccttcaagga gctgtttttg agttacagaa ccgagaagga
    19081 gaaacgctac aaaccggttt aactacagga gaagatggaa aactggcaat tgacggttta
    19141 gcaccaggag catatcagct ggtagaaac
//
LOCUS       DANKHJ010000051        18934 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000051 DANKHJ010000000
VERSION     DANKHJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(23..1846)
                     /locus_tag="PW113_001928"
     CDS             complement(23..1846)
                     /locus_tag="PW113_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2526223.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(2444..5149)
                     /locus_tag="PW113_001929"
     CDS             complement(2444..5149)
                     /locus_tag="PW113_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2526224.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(5455..6402)
                     /locus_tag="PW113_001930"
     CDS             complement(5455..6402)
                     /locus_tag="PW113_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2526225.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            6592..6861
                     /gene="rpsN"
                     /locus_tag="PW113_001931"
     CDS             6592..6861
                     /gene="rpsN"
                     /locus_tag="PW113_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2526226.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(7037..7399)
                     /locus_tag="PW113_001932"
     CDS             complement(7037..7399)
                     /locus_tag="PW113_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2526227.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(7410..8531)
                     /locus_tag="PW113_001933"
     CDS             complement(7410..8531)
                     /locus_tag="PW113_001933"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2526228.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(8547..8897)
                     /locus_tag="PW113_001934"
     CDS             complement(8547..8897)
                     /locus_tag="PW113_001934"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2526229.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(9027..10538)
                     /locus_tag="PW113_001935"
     CDS             complement(9027..10538)
                     /locus_tag="PW113_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2526230.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(10860..12212)
                     /locus_tag="PW113_001936"
     CDS             complement(10860..12212)
                     /locus_tag="PW113_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2526231.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(12365..13441)
                     /locus_tag="PW113_001937"
     CDS             complement(12365..13441)
                     /locus_tag="PW113_001937"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2526232.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            13637..15382
                     /locus_tag="PW113_001938"
     CDS             13637..15382
                     /locus_tag="PW113_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2526233.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(15662..16099)
                     /locus_tag="PW113_001939"
     CDS             complement(15662..16099)
                     /locus_tag="PW113_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2526234.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(16083..17456)
                     /locus_tag="PW113_001940"
     CDS             complement(16083..17456)
                     /locus_tag="PW113_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526235.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(17778..18266)
                     /locus_tag="PW113_001941"
     CDS             complement(17778..18266)
                     /locus_tag="PW113_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526236.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(18288..18890)
                     /locus_tag="PW113_001942"
     CDS             complement(18288..18890)
                     /locus_tag="PW113_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2526237.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         5439 a         4035 c         3445 g         6015 t
ORIGIN      
        1 gtttttgatt ttaaaacttg gtttatttct tcaaatggta gctataggtg ctgacaacga
       61 tattttcacg ccaagacaat cgcaatctta accgcaaact tgtttggtta tggagaatat
      121 tctgccagcg tctgtttggt acaaattgag gaatcaacac agtagttgta tagttttgtt
      181 tcgctgcatt tttacttacg atatctacat aacggataat tggattttca atcgagcgat
      241 aagaagagtg gacgattgaa aaacggacat ctgggaaatg ctttttaaat tctgcttcga
      301 tttctttttc tttttcgaca tcttcgtcta gcgaaacatg catcgcaacg acataatcac
      361 cgatcgaacg tgcgtaattc aacgcaccga tatttacccg tgtcacattc ccgactaata
      421 caagaacggt attcccgtca taatcatgta gttttgcatc attttctaaa cgaagctgtt
      481 ctgctacttt tttataatga tcatggatct tataaaaaat gaacataacg attggcataa
      541 tgatgaagaa aggccaaatg tcccctaacc gatagacaaa caaaatcgca atgattgcat
      601 atgagatgaa cgcacccgta atattggcaa tcgatttgct taaccatctt tttccttctt
      661 ttttccattt gacgaccatc cctgtttgag ataaagcaaa tggaatgaac acgccgatag
      721 agtataacgg aatcaagcgt tctgttgagc catggaaaat aaacagcaat acgatcgaac
      781 ctagcgctag tgtgataatc ccattggaat agcctagtcg atccccacga tcttgataca
      841 tgtgaggcat gaatttatct tttgccagat tgtatgcaag aactggaaaa gcagaaaaac
      901 cggtgttagc agcaactgct aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa
      961 taccatgtcc aaagacagct ttaccaattt gagacaaaac agtgacttct ttttcaggaa
     1021 cgatcccata ccaatagttg ataaatgtga tccccacgaa gaagaaacct aagatcaatg
     1081 ccatcatcgt caatgtagca gcggcatttt ttgctcttgg cttcttaaag aatggtacag
     1141 cattactaat cgcctccacc ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa
     1201 tcaaagcgat agaaacaccc ggcacagccg ctcctggaag tgcagtagct tgcaatggca
     1261 cagcacctgt cacgatatta tataaaccaa caacgatcag taacgagatc acaaggataa
     1321 atgtgtacac cggaaataat aggaacgatg cagattccct cagcccacga agattcagca
     1381 tcatcagtag caaaacgatt gtcacagaaa tagcgacttg atgaccgtag agtgctggca
     1441 ccgctgatgt gatcgcttca gcacctgctg agacagatac tgctactgtc aacatgtagt
     1501 cgatcaataa agagccaccg gaaatcaagc cagcattctt tccaagattc tcactgctga
     1561 cgacatatgc tcccccgcca tgaggataag catggatgat ttgacgatat gatagcgtta
     1621 atgaaatcaa taaaataata acaaaagcgg caatgggtag cgaataccag atggctgcag
     1681 cagacaaagc gactaaaacg acaacgattt gttccgtacc atatgcaata gatgataacg
     1741 catcagacga caataatgcc aaagcagcaa atcgggttaa tttgtgctca tcattttcag
     1801 cagattttaa aggcttacct actaacaacc tttttaagta atccaatttc taaactcctt
     1861 tacacacaat tttacttcaa gttttatagt cgaaaattta tagtaaacaa gatgttatag
     1921 tacgctcact gaccttaaaa gtcaataaac tttaagaaaa taattggaaa aaaacagaaa
     1981 aatatcaagc gctttttcaa acgctttaat acagcaaaaa tctaacattt ttttatgggc
     2041 tttttctttg aattctataa taaaaggata gaacttgact tagaaaatca agttctatcc
     2101 tttttgatat cttaactttt ggaagagaga tgtaaggctg ctttctaccc atcattctta
     2161 ctactcgaag catagcgcag ttgtcacaac ctcttgataa acggtgttta aaagcttgca
     2221 tctgcggtaa taagcccaac caaattaaaa acataagaag ctgtttttat ttggtcgttc
     2281 ttattacttg atgcagagca gcttttttac aacctcttgg tacacggtgt tcaatcagct
     2341 gaccctcggt attagctcaa aaaaacggca aaatatgagg aactattttt ccattttttt
     2401 ctctaatact caggtcaaaa cactgatttc acaacctctt tttttaatga aaaatatggc
     2461 tctcatgcgc cgcttctttg aatttcgtca ttttttcttc gatgaacgga tagattgccg
     2521 ttcctacgat tccaaagaca acaaacaaac cgatcatgat ccagattgct ttcgtagcat
     2581 ttggccaata aattcctcct gcagattccc gtaacaagtt taccgcatga gtaaatggca
     2641 agaacggatt gatcatttgg aagaatttcc ctgagacttg gatcggatag ttcccgcctc
     2701 cgccagagat cgacagtact aagatgataa tagcgatccc tttaccgaca ttatcgaata
     2761 atgcagccaa gacatagacg atcatcatga acgctaaggc gatcaagaca gcaaatgaca
     2821 cgctgtagac cggattttgg acatctactc ctagtaagaa aatattccct aatgtaacga
     2881 tcaagctttg cgcaatcgcc attgtcaaaa atgtcagcat acgagcaacg aattgttctc
     2941 gtttcgtaaa attgtttcgg tctttctttc ctaaatagta ttctgtcgta gcaacacttg
     3001 aaagaagaac agcgccgacc cacagacata gtgcagtata aaacggggtg ctagctgaac
     3061 cgttatttgc gattggataa agcgcattgg tttgtagctc aacaggttgt gtaaagaagt
     3121 cactttcttt tgttgcgtct agtttcaata atttgatgac ttcgcctaga tcgacttctt
     3181 gttcgccttt tcggatcgcg tctgctgctt tttggatacc agctttgata tttggccagt
     3241 cattgttgat caagtccgca gcagcgttca cagctgtttc gacttctggc attttttcat
     3301 ttactgttgt caatgtaccg gtgatttcat tttttagtgc tggccaatca ttttggataa
     3361 aatcagcggc caatcctaat ttttgctgaa tgatcggtaa ttcattgttg tataggtctg
     3421 ccccttcatt gatgccattg acgatggtat ccatatttcc attcagcatt gtattggcat
     3481 catggatttc ttgtccaatt gcaggcattt ctttttgata tttttctaaa attgccactg
     3541 cattcgttac agttgctttc gttgagctga gaagcgaatc aaagtcgatc tggtccgctt
     3601 tgctcagtgc gccttgtgca gtcccaatcg tatcgatgac ttggtctagt agagaagtga
     3661 cagtggaact gatttgacct gtattgatac cagcaatgat gccattgata ttattaacaa
     3721 cggcatcaat ttgatctagc aattcatcaa tagccttcgt gtcttcattt tgaacagctg
     3781 tgttcaaagc agataggcgg ttttgcagat tgcttagcaa gggttttaat tgatttagct
     3841 gatcaataat tggctgtaat ttcgtatttc cagcacttgt ttgcaattta gtcaggaact
     3901 cgaccagttt atcgattgca ttttgttgtt gagatattcc gtcaataaag ccgttcaata
     3961 cattgctgat gtttttcttt tcatcatcag tcagcttacc atcttcaata gctgttctta
     4021 cagtatctat taacgaacct gtcgttgttg ccacttgtcc aattgacttc aatgtaacat
     4081 ctacactgtt tgtgatgctt ggtatcgcat ctttcagctg agaggcacct gattttgttt
     4141 ggtttgctag atcacttgcc tgattttcta aatttttcac atctggcaag atatcttgta
     4201 cttgttggat gatcgtcaag ccttgttttg cttcatcgat tccttggttc attgtctctt
     4261 ggatcgaagc aaaatcgccg tcgatctctg ctagttgctt accggcattt tggatctctg
     4321 ggattttttc ttgcaaatca aggatgattt ttgcttgatc tttcaactca ggcattttgt
     4381 cattcaatgc aacgactttt tcacccatct catctacttt agggatgtaa tccacgaatt
     4441 catttgcttt attcagtttt tccttgattt caggtagttg actttgtaat tcgaggactt
     4501 gttttgtgta gccgtcgatc gtgtccaaat tttcatctgt tgataaaatc atatctttaa
     4561 ctttattgat acttactagg ttcgtatcga tgtcgtaacc tatctcattg aatactttca
     4621 ataaagtcga actggctgtt ttgataaatt catttgtgat ttgcgattgg atcgaacttg
     4681 ctcctttatc ggtgattttc ggtgcaatcg cattgatttt ttcatttacc gtatattcga
     4741 tttttggttt cttgatatca ccactagtaa aactcagcag atcttctgag aaatcctttg
     4801 gcaagtaaat acctgcgtaa tactttccgg aacggactcc gtcttctaat tgttcttttg
     4861 aatcaacgaa ttgccatcct aattgtttat ttttatgaag cgattcgatg acttgctccc
     4921 caatggccac ttctttacct tgaaactctg ctggcttgtc tgcactgtaa acagcaattg
     4981 gcagctctcc agtatttcca tatggatccc ataatgcttt gatgttaaac cacgcatata
     5041 gcgaagggat gatcatgatc gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc
     5101 gtttccagtc aagcaaatat aatcgaagcg tatttttgat tgactgcata gttctccccc
     5161 ttcttctttt ttcagctgaa ttactttacc atgtatttca tacttttttc aatcggcttt
     5221 tttcaaaaaa gtcgaatttt tggacatatc acggcaattg tctcattgta atctcttaca
     5281 tattgagaaa taattaagga ttacagcctt tactttatca tgaaataaaa gggaaagata
     5341 attttatgcc aaagttttta tctattatac gtatacgcaa aaaagctaaa acattttttg
     5401 tctttgcccc tacaaaaatg tttcagcttt tttcgttttt tattcctgta tttcttattt
     5461 ttcgaatgac aggatgattt tcccagcagg aggatgtcct tcaactagct gatgtgcttg
     5521 gatgacatgc ttaagtgaag cagacagatt cattgaaata aaaacatgaa aggctccttt
     5581 ttggtaggct ttgattatgc cagcaaaggc ttctgcatct agatattcct ttcttggaac
     5641 aaacgattca taaaagcctt cctttttttg ccgcaaatct agatctggga ggtcatttaa
     5701 tgcaacatac ctgccgcctg gtttcatgat ctggatgccg gtttctcctt tgatacttcc
     5761 tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat tgcagacctg gatttgtttt
     5821 atcataagca gcaaatgctg aagctcctaa tttttttacc ttttcctcat ttttactgga
     5881 tgcactggtt aagattcgga ttcccttttc atggagaagc tggatcagac ttgacccgac
     5941 cgcacctgat gctcctagta tcattacagt atctgtcggc tggatttccg tcaaatggtt
     6001 gattagatta taagccgtaa ttcctggcgt gaccattccg gctgcttctt cccaactcat
     6061 tttatctgga atttttgcta cttttgctga tggcaacacg acttcttctc cgtaagtccc
     6121 gccgactgca tggacagcta ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat
     6181 ctctgtcaca ataccagcgc catcattacc tggtacataa gggaatttca atgttcgtat
     6241 ctctttcatt tctcctaaac gcaaggcaac atcataagga ttcacactga atgcttttat
     6301 ttctacacga acatgtccag aaccgacttc tctgggatga gcatcgattt cttcaaaaac
     6361 atcttgtgct tctccgtaac cgttaattgc aaatcttcgc atggtcacca tctccctttt
     6421 tctttaagtt tacgtttatg cctttatttt gacaaaaaaa atgtcttttc gttttggcgc
     6481 atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa
     6541 gcgtaaagat tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa
     6601 aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta
     6661 cgccaggaat taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct
     6721 cgtccagaac gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt
     6781 aaattcggga tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga
     6841 gtaaaaaaag ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg
     6901 accactcctt ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact
     6961 agaagcagac gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat
     7021 ggttacttcg ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt
     7081 tcaatgctcg gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca
     7141 atcttgattt atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc
     7201 ctgttccagt agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg
     7261 ttatggccgc aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca
     7321 aaactgggcg tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa
     7381 tatctcctct ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt
     7441 cctaatccgc aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata
     7501 gtgatttctt tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct
     7561 tgaggcaatc ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc
     7621 ccttctacca atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc
     7681 ggtatcgttc cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct
     7741 tcacctgctg ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc
     7801 aatggataaa cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca
     7861 taacgaatca tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca
     7921 tgtacatatc gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc
     7981 agattccaat atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc
     8041 cattctaata cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta
     8101 caaaatccga tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct
     8161 agcttacttt tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt
     8221 actgataaat catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata
     8281 aggatgggct gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca
     8341 caaaagcccg aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg
     8401 tatccgttcg cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca
     8461 tttgctacat tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa
     8521 gcgacaacca tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt
     8581 tcatttgtat gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca
     8641 ggctgaccat tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca
     8701 gttccccaag ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact
     8761 tggcgatgaa aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca
     8821 atgaattttg gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc
     8881 ccaattcctt ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac
     8941 caaattataa ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt
     9001 aaaaaagaga aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt
     9061 tttttatcat tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg
     9121 ccttttgatt tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca
     9181 ccacgtttgc catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg
     9241 atcttcactg gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact
     9301 aaatcttgtg cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga
     9361 ccattttctg caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat
     9421 gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc
     9481 acatgcaagt agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg
     9541 gcacggcctg tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag
     9601 ttgtacacat gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca
     9661 aggatatcta aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac
     9721 aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg
     9781 tctacacggc gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac
     9841 aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc
     9901 aagtcagctg tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta
     9961 acgccaatat tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga
    10021 tcaggaactt gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca
    10081 tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg
    10141 tggtcaagca tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg
    10201 atctgacggc caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc
    10261 cctaaacgat ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact
    10321 aaaccttgta attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca
    10381 gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt
    10441 ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc
    10501 aacaattctg gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt
    10561 taactgagga tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt
    10621 ctttttgaca gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa
    10681 aatcactttg cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat
    10741 tattatgaca tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc
    10801 atctttcggc aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt
    10861 tatttcattt gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt
    10921 agcataatac tatctgttgc ttcaagattt tgtttgatag catcaatcaa tttattcttt
    10981 tctttttcaa aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt
    11041 gcatgcattt gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca
    11101 ctcatttgcg catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca
    11161 gcgattctgc gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct
    11221 gttggatttg cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat
    11281 tcggttctgt tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac
    11341 agtccaaaga agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca
    11401 ggcagcggga tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt
    11461 gctgtcactt ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact
    11521 aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct
    11581 gatacaagtc cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga
    11641 ttttctatgt gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt
    11701 gaaagttccg tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta
    11761 tcttggggca tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc
    11821 gtcttatatg ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt
    11881 ttaccattgc ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt
    11941 gccaattgct gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga
    12001 gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg
    12061 ataaattcat gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc
    12121 ttccggctgt caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg
    12181 gcttgtgcaa tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa
    12241 aaaagcgttt ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta
    12301 ttcgtattta gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt
    12361 ctctttattc atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact
    12421 ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt
    12481 acatactgaa ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt
    12541 tatttgtcaa aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta
    12601 acttcgcgta ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca
    12661 tttcgatttt attattgata tattttgctt cataatcata gaatgctacg tcttttacga
    12721 cttcaccagg caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc
    12781 gcgcttcaat tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag
    12841 cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg
    12901 ccggtttgac aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag
    12961 gattttcttt ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag
    13021 cttgtaaaat atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg
    13081 cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt
    13141 ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga
    13201 tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga
    13261 catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg
    13321 taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg
    13381 cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca
    13441 aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa
    13501 acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg
    13561 tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag
    13621 aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg
    13681 agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt
    13741 aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt
    13801 tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt
    13861 acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt
    13921 atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc
    13981 tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat
    14041 cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata
    14101 cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt
    14161 gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa
    14221 caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt
    14281 gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa
    14341 aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct
    14401 tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca
    14461 tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa
    14521 aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc
    14581 agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct
    14641 ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc
    14701 ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag
    14761 tcggttccac caagaactga caagttttct tgaacagtat tatgattctc ctattgatga
    14821 aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca
    14881 gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg
    14941 cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt
    15001 tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg
    15061 gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc
    15121 gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga
    15181 aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc
    15241 cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt
    15301 tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat
    15361 gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata
    15421 agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga
    15481 agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta
    15541 ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt
    15601 gatgtattcg agcataaata aaataccttc gttacctact atcttctgta cttggtaaac
    15661 cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg
    15721 gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt
    15781 ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca
    15841 cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct
    15901 tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga
    15961 tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca
    16021 cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc
    16081 catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat
    16141 ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct
    16201 atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct
    16261 tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt
    16321 tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa
    16381 tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct
    16441 atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc
    16501 atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca
    16561 atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt
    16621 atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa
    16681 gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac
    16741 acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg
    16801 aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca
    16861 agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga
    16921 taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct
    16981 gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct
    17041 gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg
    17101 accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa
    17161 taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc
    17221 agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa
    17281 aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta
    17341 tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag
    17401 ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca
    17461 atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa
    17521 taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc
    17581 tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct
    17641 tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc
    17701 aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt
    17761 taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat
    17821 ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc
    17881 tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc
    17941 gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta
    18001 tttagcaaat ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa
    18061 ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt
    18121 tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg
    18181 atcggcacaa aaacttccgg ccatacttct tgtatgactg ccatcagctc atccagttca
    18241 ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct
    18301 tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga ccacggtttc
    18361 aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga
    18421 cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata
    18481 tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata
    18541 agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc
    18601 actaggccaa tacaactata taaaagccca aatccattgc caaacatcag catgcccgct
    18661 actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca
    18721 aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat
    18781 cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata
    18841 atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc
    18901 cacatctttt ttcatttatt aaaactgcct cctt
//
LOCUS       DANKHJ010000052        18923 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000052 DANKHJ010000000
VERSION     DANKHJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            591..1280
                     /locus_tag="PW113_001943"
     CDS             591..1280
                     /locus_tag="PW113_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526238.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="PW113_001944"
     CDS             1267..2475
                     /locus_tag="PW113_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2526239.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="PW113_001945"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="PW113_001945"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2526240.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PW113_001946"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PW113_001946"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2526241.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="PW113_001947"
     CDS             complement(6578..7273)
                     /locus_tag="PW113_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2526242.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PW113_001948"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PW113_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2526243.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="PW113_001949"
     CDS             complement(8472..8663)
                     /locus_tag="PW113_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2526244.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="PW113_001950"
     CDS             complement(8684..9574)
                     /locus_tag="PW113_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2526245.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="PW113_001951"
     CDS             complement(9561..10328)
                     /locus_tag="PW113_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2526246.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PW113_001952"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PW113_001952"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2526247.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PW113_001953"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PW113_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2526248.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PW113_001954"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PW113_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2526249.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="PW113_001955"
     CDS             14899..15282
                     /locus_tag="PW113_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526250.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="PW113_001956"
     CDS             complement(15450..16208)
                     /locus_tag="PW113_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2526251.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="PW113_001957"
     CDS             complement(16226..17044)
                     /locus_tag="PW113_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2526252.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PW113_001958"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PW113_001958"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2526253.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PW113_001959"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PW113_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2526254.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..>18923
                     /locus_tag="PW113_001960"
     CDS             18172..>18923
                     /locus_tag="PW113_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2526255.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNE"
BASE COUNT         5325 a         3890 c         3468 g         6240 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gat
//
LOCUS       DANKHJ010000053        18757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000053 DANKHJ010000000
VERSION     DANKHJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1899
                     /locus_tag="PW113_001961"
     CDS             <1..1899
                     /locus_tag="PW113_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2526256.1"
                     /translation="FIVSGKAYARLDKVGADSYISQLTVKAKTMGEGEQSEMIASLNQ
                     LIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALIGMIPEGLYLLTTIALALS
                     ATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMSVQKVIVSKKQEIISQSDQ
                     LEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPFSSVQKFSSVALADKVYVL
                     GAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEEPVLTEAVTPLGYILIANP
                     IRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGISHADQYVDASKLSEEEYEA
                     AVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDILAMKEADCSIAMASGNEA
                     AMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVKNIFSFLLSLFSVIFALT
                     YPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVMEKAVPGGLTDMIVVGAL
                     VICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFRSQIILFCASGNFFSVIF
                     LHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFERKNEKEQKPFSRVVKKF
                     LSIFKREN"
     gene            complement(2002..3738)
                     /locus_tag="PW113_001962"
     CDS             complement(2002..3738)
                     /locus_tag="PW113_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2526257.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(3735..4439)
                     /locus_tag="PW113_001963"
     CDS             complement(3735..4439)
                     /locus_tag="PW113_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526258.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            4570..5073
                     /locus_tag="PW113_001964"
     CDS             4570..5073
                     /locus_tag="PW113_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2526259.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5033..5371
                     /locus_tag="PW113_001965"
     CDS             5033..5371
                     /locus_tag="PW113_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2526260.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            5392..6810
                     /gene="ffh"
                     /locus_tag="PW113_001966"
     CDS             5392..6810
                     /gene="ffh"
                     /locus_tag="PW113_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2526261.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            6922..7500
                     /locus_tag="PW113_001967"
     CDS             6922..7500
                     /locus_tag="PW113_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2526262.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            7504..8025
                     /locus_tag="PW113_001968"
     CDS             7504..8025
                     /locus_tag="PW113_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2526263.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8104..8658)
                     /locus_tag="PW113_001969"
     CDS             complement(8104..8658)
                     /locus_tag="PW113_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2526264.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            8911..9186
                     /gene="rpsP"
                     /locus_tag="PW113_001970"
     CDS             8911..9186
                     /gene="rpsP"
                     /locus_tag="PW113_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2526265.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9198..9449
                     /locus_tag="PW113_001971"
     CDS             9198..9449
                     /locus_tag="PW113_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2526266.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            9573..10364
                     /locus_tag="PW113_001972"
     CDS             9573..10364
                     /locus_tag="PW113_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526267.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            10534..11055
                     /gene="rimM"
                     /locus_tag="PW113_001973"
     CDS             10534..11055
                     /gene="rimM"
                     /locus_tag="PW113_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2526268.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11045..11806
                     /gene="trmD"
                     /locus_tag="PW113_001974"
     CDS             11045..11806
                     /gene="trmD"
                     /locus_tag="PW113_001974"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2526269.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            11942..12289
                     /gene="rplS"
                     /locus_tag="PW113_001975"
     CDS             11942..12289
                     /gene="rplS"
                     /locus_tag="PW113_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2526270.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            12599..12841
                     /locus_tag="PW113_001976"
     CDS             12599..12841
                     /locus_tag="PW113_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2526271.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            13192..13446
                     /locus_tag="PW113_001977"
     CDS             13192..13446
                     /locus_tag="PW113_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HDL2526272.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            13475..14734
                     /locus_tag="PW113_001978"
     CDS             13475..14734
                     /locus_tag="PW113_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2526273.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            14787..15683
                     /locus_tag="PW113_001979"
     CDS             14787..15683
                     /locus_tag="PW113_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2526274.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            15694..16563
                     /locus_tag="PW113_001980"
     CDS             15694..16563
                     /locus_tag="PW113_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2526275.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            16573..17248
                     /locus_tag="PW113_001981"
                     /pseudo
     CDS             16573..17248
                     /locus_tag="PW113_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            17454..>18757
                     /gene="alsS"
                     /locus_tag="PW113_001982"
     CDS             17454..>18757
                     /gene="alsS"
                     /locus_tag="PW113_001982"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2526276.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNT"
BASE COUNT         6113 a         3285 c         3912 g         5447 t
ORIGIN      
        1 ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt
       61 tcccagctga cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct
      121 tctcttaatc aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt
      181 ttttctcaaa gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa
      241 gcagcattga tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca
      301 ttaagtgcga ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag
      361 acattggcaa gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa
      421 atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa
      481 ttggaagcat gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa
      541 gctgttaaag catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt
      601 tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg
      661 cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct
      721 gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta
      781 ttgacagaag cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa
      841 gcaagagcca catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga
      901 gataatccgc tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag
      961 tacgttgatg catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca
     1021 gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgct aaagcgtcgt
     1081 aacaatacag tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca
     1141 gactgcagta tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc
     1201 ttacttgatt ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg
     1261 aacaatattg agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc
     1321 tcactgtttt cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg
     1381 attagtttgt ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag
     1441 cgaatcagag ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca
     1501 gatatgatcg tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca
     1561 gatgtttcta cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa
     1621 atctgttcac cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac
     1681 tttttttcgg tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca
     1741 cttttgctgt tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca
     1801 ttggtagaat atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta
     1861 gttaaaaaat ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga
     1921 gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa
     1981 cgcacgagca gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt
     2041 tccaagatga ctgtctacag taatcgtacc atgaaggagt tgcacgtagt cttttacaat
     2101 cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc gccttgtcta cgcggtaaaa
     2161 acgctcaaag atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga
     2221 aaacgataga tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata
     2281 ttggacagca ttttcaatca gatttttgat aattggataa aggatatctg ttttagtagt
     2341 aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg
     2401 ataattttga acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga
     2461 attctcgcca tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc
     2521 atctttttgc atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc
     2581 cagtaaagtc tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac
     2641 gttgccaacg aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc
     2701 cacccctaga atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa
     2761 tgtctgccga ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac
     2821 ttgatgaatc agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg
     2881 ttgatagctt accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga
     2941 atcagcatca tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg
     3001 ctcttctgtg gtagtatacg cccgatacat cttactcatt tgttcgctca acgcattgat
     3061 cgtctgatac aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg
     3121 attttgcacc atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata
     3181 gatcagataa tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac
     3241 ccaacggcga aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa
     3301 aatggcttct aactggccac tttttttgac tggtaatgcg acatataaaa gatccccatt
     3361 caaagtagta cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat
     3421 ttcgggacgg gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt
     3481 tccagtagca tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc
     3541 agttttgttt tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc
     3601 tttttttgtc aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat
     3661 caattgccag ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata
     3721 aatttttttc catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc
     3781 accaagtact ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt
     3841 acatctacga ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg
     3901 tctcggtcga tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc
     3961 ggtgttagtt caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg
     4021 atttgtccaa tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca
     4081 attcgtctga atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc
     4141 aaatagtcgt cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc
     4201 gtcagcatca aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg
     4261 ccatccatcg ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct
     4321 aattctaatc cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa
     4381 ttgaacgtca agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt
     4441 caaatctcct ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg
     4501 gctattttac aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa
     4561 gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga
     4621 cagaaaaaag aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc
     4681 tgtccagaag tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga
     4741 aatggattcg atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta
     4801 acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt
     4861 gatctagtca tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacggg
     4921 agtttttccg gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa
     4981 aaaatgactg tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat
     5041 cgaaaagacc aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa
     5101 acaaatgaac tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga
     5161 agaatacgaa gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt
     5221 ggaggattat gaaaaaaaac ttcatctttt ttctaattat attgttcgag aacaggtatt
     5281 ggaaaatatg actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata
     5341 tgtacagcaa attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt
     5401 gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta
     5461 tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat
     5521 gttaacttgc aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca
     5581 gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg
     5641 acgatcacac ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt
     5701 atcatgatgg ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat
     5761 tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca
     5821 gctgcgatcg accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg
     5881 ggaactgaag tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag
     5941 aaaaacgact atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg
     6001 gatgagttga aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat
     6061 gccatgactg gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt
     6121 acaggggtcg tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc
     6181 cgctcagtga caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta
     6241 gaagtattcc acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca
     6301 ttgattgaaa aagcgcagca agactatgac gagaaaaaag cggaagaact tgcacagaaa
     6361 atgcgggaaa attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt
     6421 atgggcccac tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc
     6481 gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg
     6541 acgccagcag aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct
     6601 gccggttctg gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca
     6661 agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg
     6721 tttggttcag gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa
     6781 aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga
     6841 aaatgtctac tgccggagct tttctatcag caatttttat tctttactaa tacaattact
     6901 ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg
     6961 caatatcatg atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa
     7021 ttgatgcttg atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa
     7081 gcattcgatg aagcatatga tcgattcgat attaaaaaag tcgcaaatta tggagaaaca
     7141 aaaatcagtg aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca
     7201 gccgtccaaa acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag
     7261 tatctttggt cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa
     7321 gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt
     7381 aaatttatcg gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat
     7441 caccttgaca gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa
     7501 agcatgcgaa aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa
     7561 acggatgatc ttcaatggct ttttgacact ccaacaggta agcaaaccac ttatgaagct
     7621 ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa
     7681 tatttggaga ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca
     7741 caattacaat tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat
     7801 ctacaaatga cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg
     7861 ctattggaac atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg
     7921 gacgggattc ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg
     7981 acacgattcg gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa
     8041 aaaaggcaac tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc
     8101 tttttactct gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa
     8161 acttaatgtt ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat
     8221 atatgcttcc ggttgctgca aggttggcac attcaaaaag acaagtgatt gtcgtagatg
     8281 gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt
     8341 atgatcccaa acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac
     8401 tgctggcaca gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac
     8461 ttcctcacga aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt
     8521 gtaaaatggc agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt
     8581 cgcaaaagct tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa
     8641 tccaattttt tttgtcatac gatttttcct ccttcaaact atctataaca atcttacaaa
     8701 aaataagtaa ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt
     8761 acttattttt aacaaaaaag aagtctaaaa tcttctattt cctaaaaaaa atcgaattta
     8821 atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg
     8881 tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg
     8941 ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat
     9001 ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt
     9061 ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta
     9121 cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag
     9181 aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc
     9241 tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt
     9301 acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg
     9361 ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt
     9421 tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc
     9481 agcttttttg tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc
     9541 gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat
     9601 ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat
     9661 caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggctacca
     9721 ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga
     9781 aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca
     9841 atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc
     9901 ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc
     9961 aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagcccttt gagatcgaca
    10021 caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag
    10081 gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata
    10141 tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca
    10201 gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc
    10261 gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc
    10321 ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc
    10381 tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt
    10441 gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa
    10501 ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa
    10561 atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca
    10621 gaagaacgtt acaaaaaagg aacggtgtta acactattcc aagacggaaa agcacctgtg
    10681 gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga
    10741 catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac
    10801 aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta
    10861 ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat
    10921 gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc
    10981 gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt
    11041 gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga
    11101 atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg
    11161 cgattattca gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg
    11221 tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc
    11281 ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat
    11341 ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata
    11401 tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac
    11461 tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt
    11521 gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc
    11581 ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg
    11641 aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg
    11701 tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga
    11761 aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaata
    11821 ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact
    11881 attacgatat tccgctatga gaaaagctca taagaatatt tggaataagg agaagaaaac
    11941 catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt
    12001 ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat
    12061 ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac
    12121 agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg
    12181 tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct
    12241 acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat cattgttatc
    12301 agactgagaa agaacccttg gtattcaagg gttctttttt cttagcatgt aatttgaaca
    12361 ccctgatcca gtttttttct ttaattattg gtaggggaga ataatggatt agaataaatc
    12421 tacctataag tagtagtaaa aaaaagagca ctatgataat atctttctta agagtaaccg
    12481 ctttaataaa aaatccagaa tattctaaca taaatggagt attcttagaa tcaaaaggag
    12541 aaagaagatg gtttatactt atacggagca agaaaaaatg aaagtttgag acacattcat
    12601 gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa aagaaaaaca tcctattgaa
    12661 agaagtcttg aaacgctttg accatggagt gacttataca gaaacagagg ttaattcgat
    12721 actgctgaac gtattttcta gtggcgatta tgtcgaacaa agacgttatc taatcacatt
    12781 tggctttttc aaaagaagtt cagacggtcg ggcatatcag atgatgggaa ttgaaaatta
    12841 gaagttttaa tttatactca ttgaaaaaag ttcaagtatg cgatattggt tctacaccgc
    12901 atacttgaac tttttttgtc atctgtatct ataatacaaa gccaagaacg ttattcaaaa
    12961 tatggatagc tatgataaaa gctaatgagc gattcgcagt taaataagca atcgtcagta
    13021 aaatcgttcc aggagcgtac atgaaaaaat caattggatg cgttacatga ataaagataa
    13081 ttctctttta tttgtacgaa taaataccaa aacgtacctc ctaaaattag attcaaagaa
    13141 gatagcaaag aggaaaaaga tagttctgca gcagctatag cggcatgtgg attgctagaa
    13201 ctttccaaac aactgcctgt ttcagatcct cgtcatttcg attacgaaga gatagcaatc
    13261 aaaatcttgg aagaattaca gaaaaactat acaacgaaac gagttaacgg atcaaacggt
    13321 ctgcttctcc atgccgttta tgataaaaac agcttaaaag gtgtagatga atgtgtgatc
    13381 tggggcgatt acttttatgt agaaggaatc actcgtctag ctaaaacatg gtactgttac
    13441 tggtgatgta taagagaaga ggaggattca tataatgaag cgatggaaaa aaatgatgat
    13501 tggatcaata gttttaggaa tgtcactcgt atttggtgct tgtggtaatc aagaagacag
    13561 tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg aattatgaaa caacacctga
    13621 attcaaagcg ctctttgaag cattcgaaaa gaaaacggga atcaaagtga aagcagtaga
    13681 tattgcttca gatgattatg atacgaaact caccacgatg ctttcttctg gagacacgac
    13741 agatgtattg acgatgaaaa atcttttgtc gtattcaaat tatgctttac gagatcagtt
    13801 agtcgatcaa acagaacgaa ttagagaatt agacactgat gctgctgaag aaacatatga
    13861 aatgtatgat atcgatggaa aaacatatgc attgccatat cgaacggact tttgggtact
    13921 atattacaat aagaaaatgt ttgatgatgc aggaatcgaa tatccaaaaa atctaacttg
    13981 ggacgaatat gaagatttag caaaaaaact tagtaaaaat gacggacaag tttacggagc
    14041 atatcaacat atctggcgtt cgacaatcca agctattgca gctgctcaaa acgaggcgaa
    14101 tcttgttgaa ccagattatc aatatatgac tgattattat gatcgagcat tgaggatgca
    14161 aaaagaaggc gcgcagatgg attttggcac ggctaaatca acgaaagtaa cctatcagtc
    14221 tcaatttgaa gaacaaaaag cagcaatgat gtacatggga acttggtata tggctggcat
    14281 actcgcaaac aaagaagcaa gtaagacaga agtagaatgg ggaattacag ctattcctca
    14341 gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca acagcatttg ctgtcaataa
    14401 aaactcaaag aaacaaaaag cggcgcaaga atttattgaa tttgcagcca gtgaagaagg
    14461 agcaaaagta ttagcaggag taggagtagt tccttcatat cgtacggatg agattgatca
    14521 gctttatttt aatctcgctg gaatgccaac ggatgaaatt tctaaaaaag ctttttctcc
    14581 tgatgaaatc aaacttgaat ttccgattga tacgtatggg cctgcaatcg ataagatttt
    14641 acaagaagaa tatgacttag ttttggttgg agatgaaacg ccggaaaaag gaacggtaaa
    14701 tatggagaaa cgtgtagctg cggaaaaaga ataatcatga aaggagagaa gccaagagat
    14761 aaaacactct tggtttcgtc atcgaaatgg agaatagtat aaataagaaa gctacaaatc
    14821 atttgcgtag aaaacgaacg tggatcgctt attcgtttat cgccccgaat ttcattggtt
    14881 ttttcgtttt tacattaata ccaatcctgt tttcgcttgt actagcattt atgaaatggg
    14941 attctttttc aacgccagaa tttgctggac tagataactt tcgtaaaatg gccaacgatg
    15001 agacattttg gatttctttg aaaaatactt ttttatatac cgctgctgta gtgcctggca
    15061 cacttgtttg ttcgttaggg ctagctttac tactaaatca gaaaattaga gggatgaagc
    15121 tttttcgtac gatatttttc tttccgtatg ttacttcctt agtagcaatt gctgttgttt
    15181 ggaatatgtt gtttcatcca acagtgggac ctattaatat ctttttaaga cgatttattg
    15241 aacatcctcc aggttggact tcaagttcaa attgggcgct ttgggcaatt gtaattgtca
    15301 gtatttggcg agggatgggt tattacatga ttctatacct tgcaggacta caaagtatcc
    15361 aaaaagaact atatgaagcg tcttcgctag atggagcaaa ccgttggaaa caatttttga
    15421 atgtgacatt accttcgttg agaccaacaa ccttctttgt aacgatcatg cttgtcatca
    15481 attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga tggcggccct ggtcgagcga
    15541 caaatgtgct ggtttaccaa atctacaatg aagcattcaa aaaatttgat tttggttatg
    15601 cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg cgttacggtt gttcaatttt
    15661 cttggaataa gcgtcgtact taggaggtgt gatatggaag agagtgttcg aaatgaaaga
    15721 aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc aatggttggg tatgattctg
    15781 ctgattgtat tagctttatt gacgatcatt ccttttttat ggatggtttc tgcttcattc
    15841 aaagcaaata atgaagtgtt aacgattccg attcaatgga tccctaaaac ttggcattta
    15901 gagaattatc tggttatttg gtctcgtatt cctttagtga cattttttaa gaatacagct
    15961 tttttaagcg ttgtgatcac gttgattcag ttgtttactt ctagttttgc tgtttacggg
    16021 ttttctgaac taaattttaa agggcgcgat attttatttt tagcatatat cggcacgatt
    16081 gctgtccctt ggcagtctta tatgatcccg cagttcatta tgatgagaaa agtgggttta
    16141 acagatacgt tgtggtcgtt ggtgctatta caagcgttca atgcttttgg tgtgttcttg
    16201 ttgaagcaat attacagtag tatcccagat tctttatgtg agagtgcgcg gatcgatggc
    16261 ttatcagagt gggaaatcta tcgcaagatc attttacctc ttacaaaacc ggcgattgcc
    16321 agtttgacga tcatcacatt cgttaataca tggaacgatt atatggggcc attcatttat
    16381 ttgtcttcta cggaaaataa aacaatccag ttagggttga aaatgtttat tgggctattt
    16441 gatgcagagt acgcattaat catggctgcc tcagttgttt ctgttcttcc tgttgctttt
    16501 atatttttag cgatgcaaaa atattttgta gaagggatcg cttcatcagg aatgaaggaa
    16561 taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg tttatatgga attcttttat
    16621 tggttgtatc aatcattggc aagtagcctt tgtttttggt gtgtcaatat cttgtatata
    16681 gcagctgcgt tccttttgtc gatagatgga aggaatgtct tttcctttat tttttcttta
    16741 ctttttattg gtccaggttg catcgtgatg ataggtatga cagaagaatt agcaaaagaa
    16801 aaaagttttt caccagtacg tttgttcata aagacgtttc gccaattttg gctaaaggga
    16861 tttttttatt ggctatttgc ttggatagtt agtgtgataa tgatttttga ttgtttcttt
    16921 tttatacgtt tttcttatgg aaaatggttg attccgttgt ttgttttact tgcttgtttt
    16981 acttgcttgt ttatcagttt cctttagcat caactgctgg tattttcagg tgagaaatcc
    17041 agcgtcgaaa ccaaatcaag ttctacggat agcgttctac tatacgttga aaaaatggta
    17101 tgtgagttta ctagattttt tactattgac aagtttgttt ctcttctttt tcgtaaagcc
    17161 ccaatggtgt attctactag gtccttctat cgtatttggt ttgatttact tcaacaatcg
    17221 taaattgatg agaactatgg atctgtagac tttataaatt gaaactttca attaagaatg
    17281 acagtttttt atgtaaaaac tgtcattctt tttcattttt tgaaacatat ggctaaacaa
    17341 atgaaataaa acatgctata cttttgaatg aaacaggttt agaaactgta tcacttattt
    17401 gtgattattt ctaactaata attaaaaata aagcataaaa aggacgatgt tcaatggctg
    17461 aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt gattaatcat catgttgatt
    17521 atgtattcgg tataccaggt gcaaaaatcg atggtgtatt taatgaatta gaggatcaag
    17581 gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc atttatggca caagcaatcg
    17641 gacgtattac aggagagcca ggagtagtta tcgcaacaag tggtcctggt gctagtaact
    17701 tagctacagg tttggtaaca gcgacagcag aaggtgaccc agttttagca attgccggac
    17761 aagtaaaacg aagcgatcta ttgaagttga cccatcaaag catggataat gcagcactgt
    17821 tccagccaat cacaaaatac agtgcagaaa tccaagatcc agaaacaatt tcagaagtga
    17881 ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg cgcgagcttt atcagtatcc
    17941 cgcaagatgt agtggatgca cctgtccaag gtaatgtcat caagccattg agcgatccta
    18001 aattaggttc tgcttctgca gatgatatcc gttacctagc tgaacgcatt agagaagcaa
    18061 aactaccggt actattagta ggtatgcgag gatcaagtga aaaagaaacg ttagccatca
    18121 ggcaattagt tgaaaagaca gcattgcctg ttgtagagac attccaagca gctggagtga
    18181 tctcacgtga attagaagcg catttctttg gtcgtgtggg tttatttaga aaccaaccag
    18241 gagatatgct tttgaaacga agtgatctag ttattgcaat tggctatgat ccaatcgaat
    18301 atgaagcaag aaactggaat gcggaaaaag atgcgcggat cattgtgatc gatgaagcgc
    18361 cagctgaaat cgatcctttt atgcagcctg aaagagaatt gatcggtgat atctcagcca
    18421 ctcttgattt attgacaggt tcattagagc ctcaacaagt gtcagaagat gcaaaagaat
    18481 atttagcatc tcttcaagca aaattaacgg agcgggacat tgttcaatca aaaggagaag
    18541 caggtatctt gcatccttta gaagttatta acacgcttca gtcaaaagta acagatgata
    18601 tgactgttac cgtagatgta ggtagtcatt atatttggat ggcacgccac tttagaagtt
    18661 atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac gcttggtgtt gcattgccat
    18721 gggcgatttc tgctgcattg gtgcgtccaa atacaca
//
LOCUS       DANKHJ010000054        18717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000054 DANKHJ010000000
VERSION     DANKHJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            19..540
                     /locus_tag="PW113_001983"
     CDS             19..540
                     /locus_tag="PW113_001983"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526277.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            541..858
                     /locus_tag="PW113_001984"
     CDS             541..858
                     /locus_tag="PW113_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2526278.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1015..1179)
                     /locus_tag="PW113_001985"
     CDS             complement(1015..1179)
                     /locus_tag="PW113_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HDL2526279.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="PW113_001986"
     CDS             1282..1803
                     /locus_tag="PW113_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2526280.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2263
                     /locus_tag="PW113_001987"
     CDS             1790..2263
                     /locus_tag="PW113_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2526281.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2270..2428)
                     /locus_tag="PW113_001988"
     CDS             complement(2270..2428)
                     /locus_tag="PW113_001988"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDL2526282.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="PW113_001989"
     CDS             2521..3282
                     /locus_tag="PW113_001989"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2526283.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3502..4821
                     /locus_tag="PW113_001990"
     CDS             3502..4821
                     /locus_tag="PW113_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2526284.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4837..6057
                     /locus_tag="PW113_001991"
     CDS             4837..6057
                     /locus_tag="PW113_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2526285.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="PW113_001992"
     CDS             6092..6769
                     /locus_tag="PW113_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2526286.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="PW113_001993"
     CDS             6766..7962
                     /locus_tag="PW113_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2526287.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="PW113_001994"
     CDS             7977..8300
                     /locus_tag="PW113_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2526288.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="PW113_001995"
     CDS             8304..8639
                     /locus_tag="PW113_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2526289.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="PW113_001996"
     CDS             8636..9031
                     /locus_tag="PW113_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526290.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="PW113_001997"
     CDS             9028..9399
                     /locus_tag="PW113_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526291.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="PW113_001998"
     CDS             9415..9975
                     /locus_tag="PW113_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526292.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="PW113_001999"
     CDS             10102..10413
                     /locus_tag="PW113_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526293.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="PW113_002000"
     CDS             10437..10628
                     /locus_tag="PW113_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526294.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="PW113_002001"
     CDS             10628..14473
                     /locus_tag="PW113_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL2526295.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="PW113_002002"
     CDS             14474..15346
                     /locus_tag="PW113_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL2526296.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="PW113_002003"
     CDS             15343..16392
                     /locus_tag="PW113_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526297.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="PW113_002004"
     CDS             16396..17220
                     /locus_tag="PW113_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526298.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..17783
                     /locus_tag="PW113_002005"
                     /pseudo
     CDS             17238..17783
                     /locus_tag="PW113_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         6465 a         3236 c         4205 g         4811 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtc
//
LOCUS       DANKHJ010000055        18655 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000055 DANKHJ010000000
VERSION     DANKHJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            150..278
                     /locus_tag="PW113_002006"
                     /pseudo
     CDS             150..278
                     /locus_tag="PW113_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="PW113_002007"
     CDS             392..631
                     /locus_tag="PW113_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526299.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="PW113_002008"
     CDS             743..937
                     /locus_tag="PW113_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526300.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="PW113_002009"
     CDS             1023..1694
                     /locus_tag="PW113_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2526301.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="PW113_002010"
     CDS             1747..3723
                     /locus_tag="PW113_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2526302.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="PW113_002011"
     CDS             3738..4490
                     /locus_tag="PW113_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2526303.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="PW113_002012"
     CDS             4506..5261
                     /locus_tag="PW113_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526304.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="PW113_002013"
     CDS             5274..5534
                     /locus_tag="PW113_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526305.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="PW113_002014"
     CDS             5531..7621
                     /locus_tag="PW113_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2526306.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="PW113_002015"
     CDS             7648..8112
                     /locus_tag="PW113_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526307.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="PW113_002016"
     CDS             8109..8351
                     /locus_tag="PW113_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2526308.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="PW113_002017"
     CDS             8369..8671
                     /locus_tag="PW113_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526309.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="PW113_002018"
     CDS             8741..10804
                     /locus_tag="PW113_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526310.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="PW113_002019"
     CDS             10804..13416
                     /locus_tag="PW113_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2526311.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15944
                     /locus_tag="PW113_002020"
     CDS             13413..15944
                     /locus_tag="PW113_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526312.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15994..16326
                     /locus_tag="PW113_002021"
     CDS             15994..16326
                     /locus_tag="PW113_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526313.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="PW113_002022"
     CDS             16327..16920
                     /locus_tag="PW113_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526314.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..>18655
                     /locus_tag="PW113_002023"
     CDS             17083..>18655
                     /locus_tag="PW113_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2526315.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6891 a         2925 c         3836 g         5003 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgac
//
LOCUS       DANKHJ010000056        18596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000056 DANKHJ010000000
VERSION     DANKHJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(266..1597)
                     /locus_tag="PW113_002024"
     CDS             complement(266..1597)
                     /locus_tag="PW113_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526316.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1746..2459)
                     /locus_tag="PW113_002025"
     CDS             complement(1746..2459)
                     /locus_tag="PW113_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526317.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2634..4058
                     /locus_tag="PW113_002026"
     CDS             2634..4058
                     /locus_tag="PW113_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2526318.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4357..5064
                     /locus_tag="PW113_002027"
     CDS             4357..5064
                     /locus_tag="PW113_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2526319.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            5051..5374
                     /locus_tag="PW113_002028"
     CDS             5051..5374
                     /locus_tag="PW113_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2526320.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5463..6500)
                     /locus_tag="PW113_002029"
     CDS             complement(5463..6500)
                     /locus_tag="PW113_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2526321.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6821..7804)
                     /locus_tag="PW113_002030"
     CDS             complement(6821..7804)
                     /locus_tag="PW113_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2526322.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8219..8989)
                     /locus_tag="PW113_002031"
     CDS             complement(8219..8989)
                     /locus_tag="PW113_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526323.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8982..9917)
                     /locus_tag="PW113_002032"
     CDS             complement(8982..9917)
                     /locus_tag="PW113_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526324.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10233..11882)
                     /locus_tag="PW113_002033"
     CDS             complement(10233..11882)
                     /locus_tag="PW113_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2526325.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11999..14602)
                     /locus_tag="PW113_002034"
     CDS             complement(11999..14602)
                     /locus_tag="PW113_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2526326.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14614..15663)
                     /locus_tag="PW113_002035"
     CDS             complement(14614..15663)
                     /locus_tag="PW113_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL2526327.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15689..16003)
                     /locus_tag="PW113_002036"
     CDS             complement(15689..16003)
                     /locus_tag="PW113_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2526328.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(16019..16465)
                     /locus_tag="PW113_002037"
     CDS             complement(16019..16465)
                     /locus_tag="PW113_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2526329.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16466..18388)
                     /locus_tag="PW113_002038"
     CDS             complement(16466..18388)
                     /locus_tag="PW113_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2526330.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         5618 a         3727 c         3007 g         6244 t
ORIGIN      
        1 ctaatcttta aagataaata ttaaaataac gattaataat attaacagtc tttatttttc
       61 accaccaaaa acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc
      121 ttgtgaaaaa taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt
      181 atcaaaaaat atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg
      241 ttaatttttg ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt
      301 gttcgtcaat tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt
      361 gtaaaacagc tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg
      421 tagtccaagg aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg
      481 ctactgtcaa agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata
      541 caataggcaa tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata
      601 atttagcaaa gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag
      661 caatcgtatt tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa
      721 tatgtggaat ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca
      781 aaaacggatc taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata
      841 gattagcaat agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg
      901 gctctgtaac tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta
      961 taaatgacaa taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag
     1021 actttaaaac agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag
     1081 aaagtttaat aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg
     1141 aagctcctgt ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt
     1201 caggagtaaa catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata
     1261 ccgctgcaat atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta
     1321 caactaataa tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta
     1381 acgtggtcaa tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca
     1441 ctgaattaat aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg
     1501 catctcgcaa agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt
     1561 tttcaaatac aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt
     1621 gtaatcgatt actttttcca cccaaataat acattactta tcaaaaaaag taaatacttt
     1681 taattaaata tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc
     1741 cgagcttatt tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat
     1801 accgaatact cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata
     1861 aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg
     1921 atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca
     1981 gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc
     2041 tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct
     2101 tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta
     2161 tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca
     2221 agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt
     2281 cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta
     2341 tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca
     2401 tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat
     2461 ggtgaccctc caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa
     2521 atccacatca aaattataca cacaataaaa aagatatata tatttaaaca aaactattta
     2581 ctttaagttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa
     2641 ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc
     2701 aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag
     2761 aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc
     2821 aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta
     2881 tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt
     2941 ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact
     3001 tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag
     3061 tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt
     3121 gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt
     3181 ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac
     3241 ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg
     3301 ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag
     3361 cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt
     3421 tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga
     3481 aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg
     3541 agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag
     3601 agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg
     3661 aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca
     3721 tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg
     3781 atacagggca aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact
     3841 tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg
     3901 attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta
     3961 ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa
     4021 atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag
     4081 tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata
     4141 cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag
     4201 gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt
     4261 aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat
     4321 tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa
     4381 actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt
     4441 ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg
     4501 atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg
     4561 ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg
     4621 attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg
     4681 acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac
     4741 tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt
     4801 gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc
     4861 atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct
     4921 aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt
     4981 tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg
     5041 tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc
     5101 aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc
     5161 gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag
     5221 tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc
     5281 agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg
     5341 aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa
     5401 ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt
     5461 gcttatccgt tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg
     5521 tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat
     5581 gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc
     5641 gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata
     5701 gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca
     5761 tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat
     5821 acagcgacat actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga
     5881 tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt
     5941 gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg
     6001 cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc
     6061 agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg
     6121 gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg
     6181 tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca
     6241 gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag
     6301 atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca
     6361 gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt
     6421 tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc
     6481 attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata
     6541 cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca tcataattat
     6601 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
     6661 ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa
     6721 aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
     6781 ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg
     6841 ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat
     6901 tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga
     6961 aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta
     7021 gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa
     7081 atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt
     7141 cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt
     7201 gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta
     7261 ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa
     7321 gtaggtgact gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga
     7381 cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc
     7441 gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt
     7501 tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt
     7561 ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc
     7621 ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag
     7681 taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta
     7741 catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag
     7801 tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca
     7861 ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg
     7921 attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg
     7981 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat
     8041 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa
     8101 acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact
     8161 attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct
     8221 aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga
     8281 tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc
     8341 cgtaacggaa accggagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa
     8401 atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga
     8461 caaatgtagg cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc
     8521 ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat
     8581 acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac
     8641 tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta
     8701 ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg
     8761 catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg
     8821 aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca
     8881 aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg
     8941 tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc
     9001 gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc
     9061 ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct
     9121 tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct
     9181 tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt
     9241 ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg
     9301 atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta
     9361 ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact
     9421 cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc
     9481 atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa
     9541 agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg
     9601 tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac
     9661 tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa
     9721 ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt
     9781 gtagacttcc cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa
     9841 agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt
     9901 tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat
     9961 ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag
    10021 gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac
    10081 aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta
    10141 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt
    10201 tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt
    10261 tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa
    10321 actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca
    10381 aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc
    10441 tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc
    10501 gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt
    10561 aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt
    10621 ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt
    10681 gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg
    10741 atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat
    10801 gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct
    10861 tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg
    10921 caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc taattctccc
    10981 agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa
    11041 tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct
    11101 tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta
    11161 gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct
    11221 gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc
    11281 actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact
    11341 tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa
    11401 cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc aaaattatga
    11461 tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc
    11521 tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa
    11581 cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact
    11641 tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac
    11701 ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa
    11761 ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct
    11821 aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc
    11881 atatccctct ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa
    11941 agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt
    12001 atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt
    12061 tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt
    12121 cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat
    12181 aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat
    12241 caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt
    12301 tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga
    12361 actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct
    12421 cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc
    12481 gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta
    12541 tgattaattg attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat
    12601 aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct
    12661 ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt
    12721 tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac
    12781 gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca
    12841 gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt
    12901 ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt
    12961 tatatacttt agctgtttct aattttagag atagttctgt atctccttct aatggtgaat
    13021 aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc
    13081 tatctccaac tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac
    13141 ttatctcagt tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt
    13201 caacaacagt gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat
    13261 aagctggttc atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt
    13321 ctggaaaata aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct
    13381 ttgaatcagc tactacatgc acgattttcg atccactata attctcagtc gttgtgttga
    13441 aaacaaggac ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt
    13501 tttcgattcc gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa
    13561 cattatctga aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc
    13621 ataaccgacg aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga
    13681 gcggctcaac tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg
    13741 ttcttactga gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt
    13801 tatattcagg taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa
    13861 taatattgtg gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa
    13921 tcattgactt ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat
    13981 gaagcatttc aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg
    14041 cgtctaaggt catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt
    14101 tatctcccaa tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc
    14161 ctcgccaaca catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat
    14221 tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg
    14281 tatcactgat tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat
    14341 ctgtttgtgc ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa
    14401 ccttaagctt ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta
    14461 aacaaaagtt tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat
    14521 cagacaatac gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt
    14581 gaattatatg tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac
    14641 ttgttgttct tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac
    14701 tcccccaata acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat
    14761 aaaggttgaa agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac
    14821 tgcagtgccc accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat
    14881 cgtcccttca gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc
    14941 cgattcatca taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat
    15001 agcaattcct aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac
    15061 cccaaaagca tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc
    15121 taaaatagcc gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag
    15181 tcctgttgtc attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac
    15241 taatgtagta acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac
    15301 ttttactttt ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc
    15361 aataaatccc ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc
    15421 gggacggtca gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa
    15481 tcccgtctga ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc
    15541 tgtgccacca aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat
    15601 catataggaa accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc
    15661 cattagtaac tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa
    15721 tcgtctcttc accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt
    15781 caaagcgacc tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg
    15841 aaacatccga aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa
    15901 tatctaaccc tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg
    15961 caatccctgc gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt
    16021 aaaatgatag agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa
    16081 actcgtcatc catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt
    16141 tatcagtgtc ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc
    16201 cccattcaac agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc
    16261 ttttgccatg aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt
    16321 caaatacgtc tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt
    16381 tcgataattt ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat
    16441 ttatattgat tttttcactg atcatttata agccttcttt caatatataa actaattttt
    16501 caaacgacga actcgttttt aaatttgcta ataactcatg attcgaagcg atttcattga
    16561 atgtttcata aattttattc aattcaggtt ttactttttc attaagtgca aaaaagaaaa
    16621 cagaatgaac aagtgtccca tcccaatcaa ttccttctgg attaacgtag acacagatta
    16681 ctggcttttt tataaaatct acttcgatat gcggcatagc aaaatcattc atcgctgttg
    16741 aagatagttt ttctcttttg attgctccct ctagtatccc ttctttcgcg taattttctt
    16801 ttaccaattt tttggtaatt atatttaaaa cgttcacttg attatccttt atttggtggg
    16861 taaaaaagca aaatcgttta tctaataaat ttagaaaagc atcttctgtt tgttcattta
    16921 actgtagata ctgttttact tttctgacat ctgcccctaa catcaaagga ctaacaacaa
    16981 taacaggaaa atttgtattt tcaatcggaa tcgtgcttat aacaagttca tcagtgactt
    17041 ttctatcttg tagttcattg atgcttagta catcttctat ctcaaccata tctgcaaaac
    17101 cgttatttat tctttgctct aataattttg atgttccata tccgctacta caaactaata
    17161 tcactttcgt tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt
    17221 aagcgatttc atcttcatta aaatgtatac agaatctctc ttcgatttcc tctaataaat
    17281 ctacacttat cataaatgac cttttaaaag aatatttgat tttgtccgta taagggttat
    17341 agatatttac tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat
    17401 tgatcaattc ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct
    17461 caacacccat atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt
    17521 tttcaatcga agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg
    17581 aaaatgcttt ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag
    17641 aattattact acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt
    17701 ggatagctaa agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc
    17761 gattaagcaa ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac
    17821 caatgaaatt gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac
    17881 cccaataata gttcacaact tctgacatca accttctttt acttttttca tcagctttga
    17941 tgcgcacgcc atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa
    18001 catctttcat tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt
    18061 gaatctttag agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct
    18121 ccggagagtc tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt
    18181 ttccaataag cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt
    18241 ggattatttc tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt
    18301 gttctgataa ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca
    18361 tttgccgttc gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta
    18421 caattattat ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat
    18481 attcttgatt atttcaaatc aataaaaaag cctgcaatat ttatatcaca ggcttttttc
    18541 tctattaaat atatataaga taatacattt ttctactagt tctcactaat cttgta
//
LOCUS       DANKHJ010000057        18157 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000057 DANKHJ010000000
VERSION     DANKHJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            70..360
                     /locus_tag="PW113_002039"
     CDS             70..360
                     /locus_tag="PW113_002039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526331.1"
                     /translation="MKLTDATNYMSELSSNLETIDNLIKDLNSSYNMNDQVRVYLEKD
                     DVTLDYWAKSKSFNVSFEKEIIISALKDQRKVLYTELIKTKEFISELLEEKQ"
     gene            357..638
                     /locus_tag="PW113_002040"
     CDS             357..638
                     /locus_tag="PW113_002040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526332.1"
                     /translation="MKTSYKQLDELISLKNRMKHNKTHLKYITECIDEVKKSSGDPYR
                     IKVSYDNHYLTLVSITKEQVINNLEKEKLKLTTAILEIQDEIIEILEEK"
     gene            638..910
                     /locus_tag="PW113_002041"
     CDS             638..910
                     /locus_tag="PW113_002041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526333.1"
                     /translation="MKYSLEQIDEIRSTILRIKLKRDELEELLDELNDIAKDPEERYV
                     LSFYYTREERLFEIPSVRLIDYYNTKIINLKSEIISLEKRLQKLVK"
     gene            1032..1298
                     /locus_tag="PW113_002042"
     CDS             1032..1298
                     /locus_tag="PW113_002042"
                     /inference="COORDINATES: protein motif:HMM:NF037583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526334.1"
                     /translation="MSRKLMQRNVTSTKVEFSEVIVQDGAPTIVPCESVVLTGKLSEE
                     KALSAIKRKNPDKNVVVTNVSHETALYTMPVDKFIELADKSTQA"
     gene            1337..1708
                     /locus_tag="PW113_002043"
     CDS             1337..1708
                     /locus_tag="PW113_002043"
                     /inference="COORDINATES: protein motif:HMM:NF037548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526335.1"
                     /translation="MEIVKSTFDSQTPEGMLQVFNATNGASIPLRNAIGEVLELKDIL
                     VYSDEVSGFGGAEPSQAELVAFFTEDGKTYAGVSAVATKSAKNLIDMMSANPDIKPKI
                     SFVEGKSNGGQKFVNLQVVSL"
     gene            1792..2637
                     /locus_tag="PW113_002044"
     CDS             1792..2637
                     /locus_tag="PW113_002044"
                     /inference="COORDINATES: protein motif:HMM:NF017271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526336.1"
                     /translation="MTVRISKNDRAKLEKIYGKSNKARKKYNRLRQKGVEERQLPTVP
                     TSKKSLIDYVKSTNMSRSDFNKLLDELVDFAQPYNENYIFEINKRNVAISRAQIKEAQ
                     IKTEQAQKAKEEHYKELNQLQIKKPTENTIVTPTILTELGADLPFQAIPDFNIDSFTS
                     PEGVQSYLENIGKQDEQYFDERDQLYYDNFRQAMFTIFNSDADDIVRLLDSMGLDLFM
                     KTYVTNFLDMNLDYIYDEAEVQQKKEQVYSKIAKVIESETGGEVPSYNPTKNITINSE
                     TGEEL"
     gene            2634..4355
                     /locus_tag="PW113_002045"
     CDS             2634..4355
                     /locus_tag="PW113_002045"
                     /inference="COORDINATES: protein motif:HMM:NF015154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526337.1"
                     /translation="MIKKYTGDFETTTDLNDCRVWSWGVCDIDNVDNITFGLEIDSFF
                     EWCKMQGSTDIYFHNEKFDGEFMLSWLFKNGFKWCKEAKEDRTFSTLISNMGQWYALE
                     ICWEVNYTTTKSGKTKKEKSRTIIYDSLKKYPFPVKQIAEAFNFPIKKGEIDYTKERP
                     IGYKPTKDEWEYLKNDIQIMAMALKIQFDQGLTRMTRGSDALGDYQDWVKTTYGKSRF
                     KQWFPILSLGFDKDLRKAYKGGFTWVNKVFQGKEIGDGIVFDVNSLYPSQMYVRPLPY
                     GTPLFYEGEYKPNNDYPLYIQNIKVRFRLKEGYIPTIQVKQSSLFIQNEYLDSSVNKL
                     GVDELIDLTLTNVDLELFFEHYDILEIHYTYGYMFKASCDMFKGWIDKWIEVKNTTEG
                     ARKANAKGMLNSLYGKFGTNPDITGKVPYMGEDGIVRLTLGEEELRDPVYVPLASFVT
                     AWGRYTTITTAQKCFDRIIYCDTDSIHLVGTEVPEAIDHLVDPKKLGYWGHESTFQRA
                     KFIRQKTYVEEIDGELNVKCAGMPDRIKELVTFDNFEVGFSSYGKLLPKRTQGGVVLV
                     DTMFTIK"
     gene            4368..4538
                     /locus_tag="PW113_002046"
     CDS             4368..4538
                     /locus_tag="PW113_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526338.1"
                     /translation="MDLYKAIFIVRDEGTIDGYATEHFVDISLHDFEEIYGKETREIE
                     AITLVKTGNFKK"
     gene            4806..5096
                     /locus_tag="PW113_002047"
     CDS             4806..5096
                     /locus_tag="PW113_002047"
                     /inference="COORDINATES: protein motif:HMM:NF022859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526339.1"
                     /translation="MEIKEHEAILNGILESVTDGEARSKIVEHLEALREDYGATTEAL
                     TTANSTLDKLKKDNEALVISNSKLFRERANVEQPENNEPETDQNITLDDLGI"
     gene            5109..6437
                     /locus_tag="PW113_002048"
     CDS             5109..6437
                     /locus_tag="PW113_002048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526340.1"
                     /translation="MADKITEQDVLNATNVETPVQLMTAIYNSSSSLFQANVPMPNAD
                     NIQAVGAGITRLDVVKNEFISTLVDRIGKVVIQYKSWRNPLKMFKKGNMPLGRTIEEI
                     FVDIAQEHKFNPDESVTGVFKQEIPDVKTLFHEINREGYYKQTIQEAWLEKAFTSWDN
                     FNSFVAGVMNALYTGDEVSEFEYTKLLISNYQEKELFKEIEIGEITESNAKEFIRKIK
                     STSNKLEFMSSTYNAQGVKTSTPKSDQYVIIDADTDATVDVDVLAAAFNMSKTDFVGH
                     KIVIDEFPKKQGEESSNIVAVIVDSEWFMIYDKLYKTTSLYNPEGLYWNYWLHHHQLY
                     SSSQFGNAVAFVKTATKPVTKVAFASATDTVVKGATKDIALTFTPAEATNHQGEVVSS
                     APTLVKATVKETSGKATAVTVEGLEVGQSLVTFTAIGGQQATVLVTVTSD"
     gene            6449..8263
                     /locus_tag="PW113_002049"
     CDS             6449..8263
                     /locus_tag="PW113_002049"
                     /inference="COORDINATES: protein motif:HMM:NF028148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526341.1"
                     /translation="MARRYTHVKLLANVPFDNTYAHTRWCKTQQEQESYFNSFPVLNE
                     NTDCSYQRDTQLGGVFRVDKHKDALYACNYLIFKNEETYPSKWQYAFVTDIEYKNDNT
                     SFVTFEIDVLQTYRFDIGIRESFIAKEHPQLYYSNGVPFINTIEESLDYGREYTTTNV
                     TTFHPNDGVNFLVILTSEAMPVGDKEDKSGGSIVGGPSPYSYYLLPLNSSGEVYKPNG
                     AGNANFGEYMAFLTTKEPFLNKIVGMYITSYTGIPFIVDHANKTVRYNAGGSYKITLP
                     TYASDPTGTMKTFAFFCVKEARTFVPKRIDLVGNVYNYFREAFPFNVKESKLFMYPYC
                     LLEITDTKGHVMTIRPEYLTGGKLSVYVKGSLGISNKVMIEPIDYDVTNSSIITNLSD
                     KMLIDNDPNDVGVKSDYAAAFMQGNKNSLIAQEQNIRNTFRHGMGNSAMSTGGAIFSA
                     LASANPFVGLTNIMGAGQQVNNYVAEKENGLNLLAGKVADIENIPDNVTQLGSNLSFT
                     MGNFQNYYQLRFKQIKYEYATRLDRYFSMYGTKSNRVATPNLQTRKAWNFIKLKEPNI
                     VGTMSNDVLTRVKQIFSAGVTLWHTNDILNYNQDNGDV"
     gene            8278..9258
                     /locus_tag="PW113_002050"
     CDS             8278..9258
                     /locus_tag="PW113_002050"
                     /inference="COORDINATES: protein motif:HMM:NF017190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526342.1"
                     /translation="MSRRKGAGLARNNRYTAKSRPYPNEPYSSDVEEISYYEHYRRQL
                     TLLTFQLFEWENLPKSIDPRYLEIALHTNGYLGFFKDPTLGFMVCAGAEDGQIDHYHN
                     PIFFTANEAMYHKRYPVLRYDDDDDKSKCIMLYNNDLKVPTLPSLHRFALDMADINQI
                     SRVNRRAQKTPVIIQTDEKKYFSLLQAYNQIDENNQAVFVDKDMEFDQAFNVWQTNAP
                     YVVDKLRSELNEVWNEVLTFLGINNANVDKTARVQTSEVLSNNEQIESSGNILLKSRK
                     EFCDRVNRVFGDELDGKIDVKFRTDAVRQLQLAAGQSKKDQMSGGLPSAT"
     gene            9248..10084
                     /locus_tag="PW113_002051"
     CDS             9248..10084
                     /locus_tag="PW113_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526343.1"
                     /translation="MLLKRYIESFTYYQPELSRKERIEVGRKQLFDFDYPFYDETKRA
                     EFETKFINHFYLREIGSETMGSFKFNLDEYLNLNMPYWNKMFLSNLEEFPIFDDMDYT
                     IDEKQKLLNEIDTNIKANRDESKNQTKQVDQTDNRNKNTRDTGTTDSFSRNTYTDTPQ
                     KDLRIASNGNGTGVINYATNITEDLSKETTSSTGVETNNDKTNQNTRSNASEKETKNT
                     DINKDQNQTKDTITRYKGKKGNTDYADLLEKYRRSVLRIEKMIFREMNKEGLFLLVYG
                     GR"
     gene            10090..10641
                     /locus_tag="PW113_002052"
     CDS             10090..10641
                     /locus_tag="PW113_002052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526344.1"
                     /translation="MIDFNHDKRFDGLPSVFKERFSKYPHTEYRYELLLDEEVSALIA
                     YLNEVGALVNDMSGYLNYFIEHFVEKLEEITTDTLKKWLSDGTLESLINDTVFANYIK
                     EIKRLQILVAETRANSVNILLTKNKPDVADDRTFWFKIQRDNTDYGNDPIDTLRIVAI
                     NKVSGWNTATGDIYLHIKGTEAV"
     gene            10641..10937
                     /locus_tag="PW113_002053"
     CDS             10641..10937
                     /locus_tag="PW113_002053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526345.1"
                     /translation="MADIRTQLTSEDGSDNLFPVSKAVNIITNSGTNVEGELGSLKQN
                     NETMNTSVKNAVVTANQAKDAVTELNVNVGKLTNRVTALESTVGNLDGIRYVEV"
     gene            11059..11463
                     /locus_tag="PW113_002054"
     CDS             11059..11463
                     /locus_tag="PW113_002054"
                     /inference="COORDINATES: protein motif:HMM:NF023267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526346.1"
                     /translation="MTDKNIQMHDKDNNRLMPVTIAKNVLYNETNLGLTVVDIENKAN
                     EAKQTAQQAKTTADGLSTEIETATSTANQALTKAGTAQQTADQAKTTANSASNVATTA
                     KNTSDSAQKSVTDLAVRVSSLENTAIQYTVLP"
     gene            11475..11897
                     /locus_tag="PW113_002055"
     CDS             11475..11897
                     /locus_tag="PW113_002055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526347.1"
                     /translation="MANKNIQMKDSNDNNLYPTVRTENLLDLTSRAELTMTNCQLYTA
                     SDKTNAISYLGAIGMIEGMVKFTESLTNPVITTLPEGFRPLRTKRIGCMGKYFTPNPT
                     NTKEIIYVTIMTDGRVTVNDNVGKIEYLSLDNCVFPLK"
     gene            11908..13242
                     /locus_tag="PW113_002056"
     CDS             11908..13242
                     /locus_tag="PW113_002056"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526348.1"
                     /translation="MEERIDIQMNKMKEENQKNYLLHPETNPTQVVFDETLHGNENQE
                     SFNEFVDKRKMTTTIDVSAYGVIADGVTDCTPILNKLLEDKSEKGITFYFPPCERDSY
                     YRFANTIELKPDVPIVTFLGSGETTLKFETMTAFNVNIESFNIDGFALWLPQGAQSGK
                     GIFFNDTRNYNRFDFDLFVRNCTLNEGTYVVVARGRGVTFENCLFSNISQAIIKTAFP
                     DVNSMWQGNDINTRGTGFRGFFVKHNRIHFCTAIIIDNEDDYQNVINFCEISSNTIEG
                     GVSYYRGYAHNLHVQNNNHFLAYGNRNALFEFQDVDQAYIDVDVYSRNSKVEGMNSTA
                     ISRLVVVYGHYRNLRITGKLYRCQGHVITLYGGGVNFYCDLMAQEAPLTDGYRFIQTA
                     DKRVNYDGFIVRGLSNSTKVNTPMIYKAPQTVFYNRKIDHVLTGPNASNVYN"
     gene            13650..14291
                     /locus_tag="PW113_002057"
     CDS             13650..14291
                     /locus_tag="PW113_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDL2526349.1"
                     /translation="MCHWERPGKLHIEERLDLAQAYSKHIDGSGGGGVKRCYGTPIKN
                     TNLDPSSFMSGQLFGTHAGNGRPNNFHDGLDFGSIDHPGNEMIACCDGTVTHVGTMGA
                     LRAYFVINDGTYNIVYQEFSYNQSNIKVKVGDRVKNGQVCAIRDADHLHLGFTKKDFM
                     TALGSSFVDDGTWEDPLKFLGQCFGDGDNDDNNKGKDDLIYLWLSDSLNGWKF"
     gene            14308..14700
                     /locus_tag="PW113_002058"
     CDS             14308..14700
                     /locus_tag="PW113_002058"
                     /inference="COORDINATES: protein motif:HMM:NF016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2526350.1"
                     /translation="MIEYITSWLADDTHMIYGLILWLMVAMIIDFVLGFIIAKFDKEI
                     DFSSFKAKAGIIVKVAEMILVVYFIPVAVKFGAVGITMYVTMLVGLILSEIYSILGHI
                     STIDDNNNWTDYVKKFLDGTINRKDDIK"
     gene            14700..15575
                     /locus_tag="PW113_002059"
     CDS             14700..15575
                     /locus_tag="PW113_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25"
                     /protein_id="HDL2526351.1"
                     /translation="MNGIDISSHQTGIDLSKVPCDFVNIKATGGTGYVNPDCDRAFQQ
                     ALALGKKIGVYHFAHEKGLEGTPEQEGAFFLKNIQGYIGKAILILDFEGSNQKDSAWA
                     KAFLDYVYNQTGIKPWFYTYTANLNTTDFSNVAKGDYGLWIAEYGRNLPQGYSQPSPP
                     RTNNFPVVACFQYTSSGRLSGYNGNLDLNVFYGDRETWDLYVGKKSDQIVPPENKIFD
                     ATSDEFIFTLTKGSTSVFYFDGKTIFELSDPIQLDHIRGTYNHVHGKEIPSMVWTPEQ
                     FDIYLKMYDKKPVYK"
     gene            15587..16618
                     /locus_tag="PW113_002060"
     CDS             15587..16618
                     /locus_tag="PW113_002060"
                     /inference="COORDINATES: protein motif:HMM:NF017687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HDL2526352.1"
                     /translation="MTNSLGVKLKEKNLYYSPNNALGFNCLMLFVIGARGIGKTYGYK
                     KFVVNRFIKHGEQFIYLRRFKTELKKIPQFFKTMAKEFPEHKLEVKGKEFYCDDKLMG
                     WAVPLSTWGIEKSNEYPDVRTILFDEFLIEKSKITYLPNEAEALLNMMETVFRDRTNT
                     RCILLSNATSVVNPYFLYFNLQPDLNKRFNLYQDRGILIELCDSKDFAEVKKQTPFGR
                     LIRGTDYEDFSINNEFVNDSDTFIEKRSKNSSFLCAIAFEGKIFGYWIDAETGCVYVS
                     YDYQPNTNHFYAMTTKDHEENRLLMKNWRNNYYLSTVAKAFKNSYLRFDNIVIKNLHY
                     DLFNRMKIW"
     gene            complement(16791..17102)
                     /locus_tag="PW113_002061"
     CDS             complement(16791..17102)
                     /locus_tag="PW113_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526353.1"
                     /translation="MIARTFKEHRELIEWLRFYCKRNLSDNEKIEIIEGTLQDFDVPE
                     INITELFLTHSTLLPESSQFNILEKYCQAMKLVTSYVKVGSRYQLAIQIPKGYLKEKE
                     I"
     gene            complement(17108..17398)
                     /locus_tag="PW113_002062"
     CDS             complement(17108..17398)
                     /locus_tag="PW113_002062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526354.1"
                     /translation="MINNLSLILEGLNQLTKDDNDSLASIKSEITQGGNQLILYVDYV
                     TKEFVLTHDKYNYVYLDSHCINIARTKSMKSVEHYAEQLKHDGYKQITDKWE"
     gene            complement(17471..17704)
                     /locus_tag="PW113_002063"
     CDS             complement(17471..17704)
                     /locus_tag="PW113_002063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526355.1"
                     /translation="MFNKALIAEKGEVSLLRPFNVWDMNYHIIDTENNKHYRIDIDDV
                     GDEEYCLLSFEELKELDMDLISDYSWKTTKITD"
     gene            complement(17777..17965)
                     /locus_tag="PW113_002064"
     CDS             complement(17777..17965)
                     /locus_tag="PW113_002064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526356.1"
                     /translation="MYFDILIELYDGDIEKTNLFTDTDKLKTYLYKILDKEDAEFLLE
                     NGKVVIDDYTLYLDSATY"
BASE COUNT         6596 a         2814 c         3397 g         5350 t
ORIGIN      
        1 ctattatatc ctttacaaat atgtgattat atggtaaact atatttgtaa ggtaaagggg
       61 gtgaataaaa tgaaacttac agatgctaca aattatatgt cagaactatc atcaaactta
      121 gaaacaattg ataatttaat taaagatctt aattcttcat acaatatgaa tgatcaagtt
      181 cgtgtttact tggaaaaaga tgatgtcact ctcgattatt gggcaaaatc aaaatctttt
      241 aatgtttcgt ttgaaaaaga aatcataatt tcagctttaa aagatcaaag aaaagtatta
      301 tacacagaat taataaagac gaaagaattt atctcagaac tattggagga aaaacaatga
      361 aaacaagtta taaacaatta gatgaactaa tttcattaaa aaatcgtatg aaacacaata
      421 aaacacatct taaatacatt accgaatgta tagatgaagt taaaaaatca tcgggcgatc
      481 cataccgtat taaagtttcg tacgacaatc attatcttac acttgtttct atcacaaaag
      541 aacaagtaat aaataattta gaaaaagaaa agttaaaatt aacaacagcg attttagaaa
      601 tacaagatga aattatagaa attttggagg agaaataatg aaatactcac tagaacaaat
      661 agatgagatt agatcaacaa ttctcagaat taaattaaaa agggatgaac tggaggaact
      721 gttggacgaa ttaaacgata ttgctaaaga tccggaagaa agatatgttt tatcgtttta
      781 ttacacaaga gaagaacgtc tgtttgaaat tccgtcagta agattaatag attattacaa
      841 cacaaagatc ataaatctga aatcggaaat catatcactc gaaaaaagat tacaaaaact
      901 agtaaaataa ttacacaaaa acctttacaa atataacaca tcatgttata ttaaaagagt
      961 agtaagggaa cggaaaatac cttacttcac acctcaatca ttcttatcaa attacaaaag
     1021 gagggaaaag aatgagtcga aaactaatgc aacgaaacgt gacatcaact aaagtggaat
     1081 tttcagaagt tatcgtacaa gatggagcac ctacaattgt accatgcgaa tcagttgtac
     1141 taacaggaaa actttcagaa gaaaaagctt tatcagcgat caaacgtaaa aaccctgata
     1201 aaaacgtagt agtaacaaat gtttcacatg aaacagcgct ttacacaatg ccagtcgata
     1261 aatttatcga gttagcagac aaatcaacac aagcctaata aaacaaaact aaaagaaaac
     1321 agaggagatt agaatcatgg aaatcgtaaa aagcacattt gacagccaaa caccagaagg
     1381 aatgttacaa gtattcaatg ccacaaacgg agcttcaatt ccgttacgta acgcaatcgg
     1441 agaagtttta gaattgaaag acattctagt ttactcagac gaagtttctg ggtttggtgg
     1501 agccgaacca tcacaagcag agctagtcgc tttcttcaca gaagatggta aaacttatgc
     1561 aggtgtatca gcagtagcaa caaaatcagc taaaaatcta attgatatga tgagtgctaa
     1621 ccctgacatc aaaccaaaaa tttcttttgt agaaggaaaa tcaaacggtg gacaaaaatt
     1681 tgtaaatcta caagtggttt cactgtagca taaaatacag gaatctagta aaccacttag
     1741 cgaatctcgc taggtggctt ttattatgat tctacattga ggtgtgtaga attgacagta
     1801 agaatatcaa agaacgatag agcgaagtta gagaaaattt atggaaaatc taacaaagct
     1861 cgtaaaaagt acaatcgttt aagacaaaaa ggagttgagg aaagacaact accaactgtt
     1921 ccaacatcaa agaaaagcct tattgactac gtaaaatcaa caaatatgag tcgtagtgat
     1981 ttcaacaaat tattagatga gttggtagac tttgcacaac cttacaacga gaattacatt
     2041 tttgagatca acaagcgaaa tgttgctatc tcaagagcgc aaatcaaaga agctcaaatt
     2101 aaaacagagc aagctcaaaa agcaaaagaa gaacactaca aagagcttaa ccagcttcag
     2161 attaagaaac caacagaaaa cacaattgtc acaccaacga ttttaacaga gttaggtgcg
     2221 gacttacctt ttcaagcaat accagatttt aatattgatt cttttacttc tccagaagga
     2281 gttcagtctt atttagaaaa tataggaaaa caagacgaac aatattttga cgagagagat
     2341 cagctttatt atgacaattt caggcaagca atgtttacta ttttcaattc agatgctgac
     2401 gacattgttc gtttacttga ttcaatgggg cttgacctat ttatgaaaac ctatgttact
     2461 aacttcttag acatgaacct tgactacatt tatgatgaag cagaagtaca acagaaaaaa
     2521 gaacaagttt acagtaagat tgcaaaagtg atcgaatctg aaacaggtgg agaagtcccc
     2581 tcatataacc ccacgaagaa catcacaatt aattcagaaa caggagaaga attatgatta
     2641 agaaatatac aggcgacttt gaaacaacaa ctgatctcaa cgattgtcgt gtatggtcgt
     2701 ggggcgtatg cgatatagac aatgttgaca atataacgtt cggcttagaa atcgattctt
     2761 tttttgagtg gtgcaaaatg caaggcagca cagacattta tttccacaac gaaaaatttg
     2821 acggagagtt tatgctttca tggttattca aaaacggttt caaatggtgt aaagaagcaa
     2881 aagaagatcg aacattctcc acactcatct caaatatggg tcaatggtat gctttggaaa
     2941 tttgttggga agttaattac acaacaacaa aatcaggtaa aacgaaaaaa gagaaatctc
     3001 gaacaataat ttatgatagc cttaaaaaat atccttttcc agtcaaacaa attgcagaag
     3061 cttttaattt tcctataaaa aaaggcgaaa tagattatac aaaagaaaga cctattggtt
     3121 acaaaccgac aaaagatgag tgggagtatt taaagaacga cattcagatt atggcaatgg
     3181 cattaaaaat tcaattcgat caaggactaa ctcgaatgac tagaggaagt gacgctttag
     3241 gcgattacca agattgggta aagaccacat atggtaaatc aagatttaaa caatggtttc
     3301 ctattttgtc tttagggttt gataaagact tacgtaaagc atacaaaggc ggcttcactt
     3361 gggtaaacaa agtttttcaa gggaaagaaa taggcgatgg cattgtcttt gatgttaact
     3421 ctttgtatcc ctctcaaatg tatgtaagac ctttaccata tggaacacct ctattctatg
     3481 agggagaata caaaccgaac aacgactatc cactttacat tcaaaatatc aaagtaaggt
     3541 ttcgtttaaa ggagggttat attccaacca ttcaagttaa gcaaagctca ttatttattc
     3601 aaaacgaata tcttgattcg agcgtaaaca aacttggagt cgatgaatta attgatctta
     3661 cacttacaaa cgttgatctt gagttgttct ttgaacatta cgacatttta gagatacatt
     3721 acacgtatgg ttatatgttc aaagcttctt gtgatatgtt caaaggctgg atcgataaat
     3781 ggatcgaagt aaagaacacc actgaaggag ctagaaaagc gaatgctaaa ggtatgttaa
     3841 atagcttgta tggaaagttc ggaacaaacc ctgacattac aggaaaagtg ccttacatgg
     3901 gcgaggacgg cattgttcga ttgacactag gagaagaaga attaagagat cctgtttatg
     3961 ttccgcttgc tagttttgtg acggcttggg gtagatatac taccattaca accgctcaaa
     4021 aatgttttga tcgcattatt tattgtgata cagatagcat tcatttagta ggaacagaag
     4081 ttccagaagc aatcgatcac ttggttgatc ctaaaaaact tggttattgg gggcatgaaa
     4141 gtacatttca acgagcaaaa ttcattcgcc agaaaacata cgtagaagaa attgatggcg
     4201 aattaaatgt aaagtgcgct ggtatgccag atcgaataaa agagcttgta acttttgaca
     4261 attttgaagt tggtttttca agctatggaa agttgctgcc gaaaagaaca caaggtggcg
     4321 tggtattagt agacacaatg tttacaatca aataaggagg actaataatg gacctatata
     4381 aagcaatttt tattgtacgt gatgaaggaa cgattgacgg ttacgctacc gaacactttg
     4441 tagatatttc tttgcatgac tttgaagaaa tctacggaaa agaaacacgt gaaattgaag
     4501 caataacatt agtaaaaaca ggaaatttca aaaaataaat tatttacatc ctttgcaaag
     4561 tatggtaaaa tattcttgtg atagttaaca agagtcaaat ttggcgagat tgggcgaatg
     4621 tacacgtgag agatcgtgcg ctcccgttaa gttatggaca cataaacgtt ttgaccgtta
     4681 accaatcgca aaaacctttt aggagtagcc cttaaatgcg gctactcttt ttttgtgttt
     4741 cacagattta tgtttcacgt gaaacagttt ttatggtata atagaatcaa aaggaggtgg
     4801 agattatgga aattaaagaa catgaagcca ttttaaatgg tattcttgaa agtgtcacag
     4861 acggggaagc aagatcaaag attgtagaac atcttgaagc attgcgagaa gattacggag
     4921 caacaacaga agctttgaca acagcgaata gcacacttga taagctaaag aaagataacg
     4981 aagcgttggt tatttcaaac tcaaaattgt tccgagaacg agcgaacgta gaacaaccag
     5041 aaaataacga accagaaaca gaccagaata ttacactaga tgatctagga atttaaggag
     5101 gaaaaaacat ggcagataaa attacagaac aagatgttct taacgccaca aatgtagaaa
     5161 cacctgtaca attgatgacg gctatttata atagttcatc atctcttttt caagcgaacg
     5221 tacctatgcc aaatgcagat aacattcaag cggttggcgc tgggatcaca cgtttagatg
     5281 tagtaaaaaa tgaatttatt tcaactttag ttgatcgtat tggtaaagta gttattcaat
     5341 acaaatcatg gcgaaaccct ttgaaaatgt ttaaaaaagg aaacatgcct ttaggtcgaa
     5401 cgatcgaaga aatttttgtt gacattgcac aggaacataa gttcaatcct gacgaatctg
     5461 ttacaggtgt atttaaacaa gaaattcccg atgtaaaaac attgttccac gaaattaatc
     5521 gtgaaggtta ctacaaacaa acgattcaag aagcatggtt agaaaaagca tttacttcat
     5581 gggataattt caatagtttt gttgctggtg taatgaacgc tttatacaca ggcgatgaag
     5641 taagtgaatt tgaatacacg aaattgttaa tttcaaacta ccaagaaaaa gagttattca
     5701 aagagatcga aattggtgaa attactgaat caaatgcaaa agaatttatt cgtaagatca
     5761 aatcaacatc taacaaattg gaattcatga gttctactta caacgctcaa ggcgttaaaa
     5821 catctacacc aaaatctgac caatacgtta ttattgacgc tgacactgac gcaacagttg
     5881 acgttgatgt tttagcagca gcattcaata tgagtaaaac tgactttgta ggacacaaaa
     5941 tcgttattga tgagtttcct aaaaaacaag gcgaagaatc gtcaaatatt gtggcagtta
     6001 ttgtagatag tgaatggttt atgatctatg acaaattgta caaaacaaca agtctataca
     6061 atccagaagg cttatattgg aattactggt tacaccacca ccaattatat tctagttccc
     6121 aattcgggaa tgccgttgct tttgtcaaaa cagcaactaa accagttaca aaagttgctt
     6181 tcgcaagtgc aacagatact gttgttaaag gagcaactaa agatattgca cttacattta
     6241 caccagctga agctacaaat caccaaggag aagttgtttc atcagcacca acattggtta
     6301 aagcaacagt caaagaaaca tcaggcaaag cgacagccgt aaccgtagaa gggttagaag
     6361 tcggtcaatc attagtaaca ttcacagcta tcggaggtca acaagcaacg gttcttgtta
     6421 cggttacttc tgattaagga ggacaattat ggcaagacgg tatacacatg taaaattgtt
     6481 ggctaacgtg ccttttgata acacctatgc acacacaaga tggtgtaaaa ctcaacagga
     6541 acaggaatcg tattttaatt cgtttcctgt tctaaacgag aatactgatt gttcttatca
     6601 aagagataca caactcggtg gagtttttag agtagataaa cacaaagacg ctttatatgc
     6661 ttgtaactat ctcatcttta aaaacgaaga aacttatcct agtaaatggc agtatgcctt
     6721 tgttacagat attgaatata agaacgacaa cacaagtttc gttacttttg aaattgatgt
     6781 tttacaaact tatcgtttcg atattggcat acgagaaagt ttcattgcga aagaacatcc
     6841 acaactttat tattcgaatg gagtaccatt cattaatacg attgaagagt cgcttgatta
     6901 cggtagagaa tacacaacaa caaatgtaac aacttttcat cctaacgatg gagtcaattt
     6961 tcttgttatt ctaacaagtg aagcaatgcc agtaggagat aaggaagata aatcaggagg
     7021 atcaattgtc ggagggccgt caccttattc ctattattta cttcctctca attcaagtgg
     7081 cgaggtatac aaaccaaatg gtgcaggtaa tgctaacttt ggagagtaca tggcgtttct
     7141 tacaacgaaa gaaccttttt taaataagat agtcgggatg tatataacgt catatacagg
     7201 tataccattt attgtggatc atgctaacaa aacagtaagg tataatgcag gaggttcgta
     7261 taagattaca ctcccaacct atgccagtga tccaacagga acaatgaaaa cattcgcttt
     7321 cttttgtgtg aaagaggcaa gaacatttgt tcctaaaaga attgatctgg tagggaacgt
     7381 gtataactac tttagagaag cttttccatt taatgttaag gaatcaaaac tatttatgta
     7441 tccctattgt ttactagaaa ttacagatac taaaggacat gttatgacta tcagacccga
     7501 atatcttaca ggtggtaaat tgagtgtata tgtaaaaggt tcgttaggca tttctaataa
     7561 agtcatgatt gaaccaattg attatgatgt aactaactca agcattatta ctaatttaag
     7621 tgacaagatg ttaatcgata atgatcctaa tgatgtagga gttaaatctg actatgctgc
     7681 tgcattcatg caagggaaca aaaactcctt gattgctcaa gaacaaaaca tacgaaacac
     7741 ctttaggcat ggtatgggaa acagtgcaat gagtacagga ggggcgatct tttccgcatt
     7801 agccagcgcc aatccatttg ttggtttgac taacattatg ggggctggac aacaagtaaa
     7861 caactatgtt gctgaaaaag aaaatggatt aaatcttttg gcaggtaaag tagcagatat
     7921 cgaaaatatc ccagataatg tcacacagct aggatcaaac ttatctttca caatgggaaa
     7981 ctttcaaaat tattatcaat tgcgattcaa acaaattaaa tatgagtacg ctacaagatt
     8041 ggatcgttac ttctcaatgt atggcacaaa gagcaatcga gtagctacac caaacttaca
     8101 aacaagaaaa gcatggaatt tcattaaatt aaaagaacca aatattgtag gtacaatgag
     8161 taacgatgtt ttaacaagag tgaaacaaat ttttagtgca ggagttaccc tttggcatac
     8221 aaacgatatt ttaaattata accaagacaa cggagatgtg taggaaggag gaataagatg
     8281 agtagacgaa aaggtgcagg acttgctaga aataaccgtt atacagcaaa aagcagacct
     8341 tatccaaacg aaccctattc aagtgatgta gaagaaatca gctactatga acattatcgt
     8401 agacaactca cgctccttac gtttcagttg tttgaatggg aaaatttacc gaaatcaatt
     8461 gaccctcgat atttagaaat tgcattacac acaaatgggt atctcggttt ctttaaagac
     8521 cctacactag gcttcatggt ttgtgcagga gcagaagatg gtcaaattga tcattatcac
     8581 aatcctattt tctttacagc caatgaagca atgtatcaca agagatatcc tgttttaaga
     8641 tatgatgatg atgatgataa atcaaaatgt atcatgttgt ataacaatga cttgaaagtt
     8701 cctacactac cgagtcttca tcggtttgct ttggatatgg cagacataaa ccagatatca
     8761 cgagtgaatc gaagagcgca aaaaacacct gtaattattc aaactgatga aaagaaatac
     8821 ttctcattgc tacaagctta taaccaaatt gacgaaaata atcaggctgt ttttgtggat
     8881 aaagatatgg agtttgacca agcttttaat gtgtggcaaa caaatgcacc atatgtagtg
     8941 gataaactaa gatcagaatt gaacgaagta tggaatgaag tcttaacttt tctaggtatc
     9001 aacaatgcca acgtagataa gactgcacgt gtacaaacat cagaagtctt atctaacaac
     9061 gaacagattg aaagctcagg aaacatcttg ttaaaatcaa gaaaagagtt ttgtgatcgt
     9121 gtaaatcgtg tctttggcga tgaacttgac ggaaagattg acgtgaagtt tagaacggac
     9181 gcggtgcgac aattacaact agcggcaggt caatcgaaaa aagatcagat gagtggaggg
     9241 ttgcctagtg ctacttaaac gttatattga aagtttcact tattaccaac ctgaattatc
     9301 tcgaaaagaa cgtattgaag ttggcagaaa acaactgttt gattttgatt atccatttta
     9361 tgacgaaaca aaacgagcag aatttgaaac aaaatttatc aatcactttt acttgagaga
     9421 gataggatca gaaacgatgg gatcatttaa gtttaatctt gacgagtatt taaatctaaa
     9481 tatgccctat tggaataaga tgttcctatc aaatcttgaa gagtttccga tttttgatga
     9541 catggactat accattgatg agaaacagaa attgttaaat gagattgata caaacatcaa
     9601 agcgaatcgg gatgagtcaa aaaatcaaac gaaacaagtc gatcaaacag acaacagaaa
     9661 caaaaatacc cgtgacacag gaacaaccga ttctttctca agaaacacgt atacagacac
     9721 tcctcaaaaa gatttgagaa ttgccagcaa tggaaatggt acaggtgtaa tcaattatgc
     9781 aacaaatatt acagaagatt tgagtaaaga aacaacaagc tccaccggcg ttgaaacaaa
     9841 caacgataaa acaaaccaaa atacacgaag caatgcttct gaaaaagaaa caaagaatac
     9901 agatattaat aaagatcaaa atcaaaccaa agatacgatt acacgatata aaggtaaaaa
     9961 aggaaacact gattatgctg acttactcga aaaatatcgt agaagtgttt tgagaattga
    10021 gaaaatgatc tttagagaaa tgaacaagga aggcttattt ctccttgttt atggagggag
    10081 gtagtaacaa tgatagattt taaccacgac aagcggtttg acggtttacc ctcagtattc
    10141 aaagaacggt ttagcaaata ccctcatacg gaatacagat acgaattact attagatgaa
    10201 gaagtatcgg ctttaattgc ctatctgaat gaggttggcg ctttagttaa tgatatgagt
    10261 ggttatttaa attactttat cgaacatttt gttgagaagt tagaggagat cacaactgac
    10321 acactcaaaa aatggttatc tgacggtaca ctagaaagtt taatcaacga tactgttttt
    10381 gcaaattata tcaaagaaat taaaagatta caaatcttgg ttgctgaaac aagagcgaac
    10441 agtgtgaata tccttttgac aaagaataaa cctgatgttg ctgatgatcg aacattttgg
    10501 tttaaaatac agcgtgataa tactgattat ggtaatgatc ctattgacac gttacgtatt
    10561 gttgcaatca ataaagttag cggttggaat accgctacag gagatattta tcttcatatt
    10621 aaaggaacgg aggctgtata atggcagaca ttagaacaca actaacaagt gaagatggat
    10681 cagacaattt atttccagtt tcaaaagccg ttaatattat aactaatagc ggtacgaatg
    10741 tagaaggaga attgggttca ctcaaacaaa ataacgaaac aatgaatacc tcagttaaaa
    10801 atgctgtagt tactgctaat caagcaaagg atgctgtaac tgaattaaat gtaaatgttg
    10861 gtaaactaac caatagagta acagcattag agagtacagt cggtaatctt gatggtattc
    10921 gttatgtaga ggtgtaatat ggcagctaaa aacacaggtg attcaccgca aaaaaaactg
    10981 caactgatct tgctgttcga gtaagcagtt tagagaacac aacaatacaa tatactgttt
    11041 tatcatagga ggaaaaacat gacagataaa aatattcaaa tgcatgataa agataataat
    11101 cgtttaatgc ctgtaaccat tgcaaaaaat gttttgtata atgaaactaa tttagggttg
    11161 actgttgttg atattgagaa caaagctaat gaagcaaaac aaacagctca acaagcgaaa
    11221 acaacagccg atggattgtc aacagaaatt gaaacagcaa cttcaaccgc caatcaagcg
    11281 ttaacgaaag ctggtacagc gcaacaaacc gcagaccaag cgaaaacaac agcgaacagt
    11341 gctagtaatg ttgcaacgac agctaaaaac acctctgatt cagcacaaaa aagtgtaact
    11401 gatcttgctg ttcgtgtaag tagtttagag aacacagcaa tacaatatac tgtattaccg
    11461 taggaggaaa aataatggca aataaaaata ttcaaatgaa ggatagcaat gacaataatt
    11521 tatatccaac tgttcgtaca gaaaacttgt tagatttaac cagtcgtgct gaattgacaa
    11581 tgacaaattg tcaattatat acagctagtg ataaaacaaa tgcaatctct tatctcggag
    11641 caattggtat gatcgaaggt atggtaaagt ttactgaaag tttgacaaac cctgtgatta
    11701 caacgttacc agaaggcttt agaccattga ggacaaaacg tattggttgc atgggtaaat
    11761 attttacacc aaatccaact aacacgaaag aaatcattta tgttacgatt atgacagacg
    11821 gaagagtaac tgtaaatgac aatgtaggta aaatcgaata tctatcatta gataattgtg
    11881 ttttccctct aaaataagga ggttcatatg gaagaacgaa ttgatattca aatgaacaag
    11941 atgaaagaag aaaatcaaaa gaattaccta ttgcaccctg aaacaaatcc aacacaggtt
    12001 gtttttgatg aaacattaca tggaaatgaa aatcaagaaa gtttcaacga atttgttgac
    12061 aaaagaaaaa tgacaactac aatcgatgta agtgcttatg gggttattgc agatggtgta
    12121 acagattgta caccaatatt aaataaatta ctcgaagaca aaagcgaaaa gggtatcact
    12181 ttttattttc ctccttgtga acgtgattca tattatcgct ttgctaacac aattgaatta
    12241 aagcctgatg ttccaatagt gactttctta ggatcaggag aaacaacatt aaagtttgaa
    12301 acaatgacgg catttaatgt aaacatcgaa agtttcaaca ttgatggttt tgcattatgg
    12361 ttgccacaag gcgctcaaag tggtaaagga attttcttta atgatactcg aaattacaat
    12421 cgtttcgact ttgatttgtt tgttcgcaac tgtactttaa atgaaggaac atatgttgtt
    12481 gttgctagag gtagaggggt tacctttgaa aattgtctat tctctaatat ctctcaagca
    12541 atcatcaaaa cagcttttcc cgatgtaaac agtatgtggc aagggaacga tattaatact
    12601 agaggcacag gatttagagg attttttgtg aaacacaatc gtattcactt ttgtacagcg
    12661 atcattattg ataacgaaga tgattatcag aatgtaatta atttctgtga aatttctagt
    12721 aatacgatcg aaggtggcgt aagttattat cgaggatatg cacataactt gcatgttcaa
    12781 aacaacaacc attttctagc atatggaaat agaaacgcct tgtttgaatt tcaagatgtg
    12841 gatcaagctt atattgatgt agatgtttat tctcgtaact ctaaagttga aggaatgaat
    12901 agtacagcta tttcacgttt agttgttgtt tacggacatt accgaaactt aaggattaca
    12961 ggaaagttat atcgttgtca aggacatgtt atcactttat atggtggtgg cgttaatttc
    13021 tattgtgatc taatggcaca agaagcacct ttgacagatg gttaccgctt tattcaaaca
    13081 gcagataagc gtgttaatta tgatgggttt attgttcgtg gattgtctaa ttcaacaaaa
    13141 gtaaataccc cgatgatcta taaagcacct cagactgttt tctataatcg taaaattgat
    13201 catgtactaa caggaccaaa tgcaagtaac gtatataact agggggatat gagatggcaa
    13261 ctcttacaaa tgaacaaata gctagaggac aaacaattgc taaaatactt ggtaaacatg
    13321 gctataataa aaattcacaa gtaggagttg tcgctaatct ccattgggaa tcggctggat
    13381 tgaacccgaa cgccaatgaa tacggtggcg gtggttatgg cttaggtcaa tggacgccta
    13441 aaagcaatct ttatcgccaa gctcaaattt gtggattgtc taatgctcaa gctgaaacgt
    13501 tggagggtca agcagagatc atcgctcaag gagataaaac aggtcaatgg atggataata
    13561 cacctatttc ttatccagga tatactaatc ctcagaccct ctcggcattt aagcaatcca
    13621 caaatattga tgttgctaca atcaatttta tgtgtcattg ggaaagacca ggcaagcttc
    13681 atattgaaga aagacttgat cttgcacaag cttatagtaa acatattgat ggtagcggtg
    13741 gcggtggcgt aaaacgttgc tatggaacac cgatcaagaa cacaaatctc gatcctagta
    13801 gtttcatgag tggacagctt tttggtacac atgcaggaaa tggcagacca aacaatttcc
    13861 atgatggttt ggactttggt tcaatcgatc atcctggtaa tgaaatgatt gcttgttgcg
    13921 atggcacagt aacacatgtt ggaacaatgg gagcattaag agcgtatttc gtgataaatg
    13981 atggtactta caatatcgtt tatcaagagt ttagttataa tcagtcaaat ataaaagtaa
    14041 aagttggcga tagagttaag aatggtcaag tgtgtgctat acgtgacgca gatcatttac
    14101 acttagggtt cacaaaaaaa gactttatga cagcgttagg ctcatctttt gttgatgatg
    14161 gaacgtggga agaccctcta aaatttttag gccaatgttt tggcgatgga gataatgacg
    14221 acaacaataa aggtaaagat gatcttattt atttgtggct atctgattca ttaaatggtt
    14281 ggaaatttta ataaggagaa aaatggtatg atagaatata ttacatcatg gttagcagat
    14341 gatacccata tgatttacgg tttgattttg tggctaatgg ttgcgatgat tattgatttc
    14401 gtgttaggtt ttataattgc caaatttgac aaggaaatcg actttagtag ttttaaagct
    14461 aaagcaggca ttattgttaa ggtggcagaa atgattttag tggtttactt tattcctgta
    14521 gcagtaaaat tcggtgcagt aggtattaca atgtatgtaa caatgttggt tggtttgatt
    14581 ttatcagaaa tttatagtat actaggacac atttcaacta tagacgataa taacaattgg
    14641 actgattatg ttaagaagtt tttagacgga acaatcaaca gaaaggacga tattaaatga
    14701 tgaatggtat tgatatttct agtcatcaaa cagggattga tctttcaaaa gttccatgtg
    14761 actttgtaaa tattaaagca acaggtggta caggctatgt caatcctgat tgtgatcgag
    14821 catttcaaca agctttggct ttaggtaaaa agattggtgt gtaccacttt gcacatgaga
    14881 aaggcttaga aggtacacca gaacaagaag gagcattttt cttaaagaat attcaaggtt
    14941 acattggtaa agcaattcta attcttgact ttgaaggttc aaaccaaaaa gattcagcat
    15001 gggcgaaagc gtttcttgat tatgtgtaca atcaaacagg tattaaacca tggttttaca
    15061 cgtatacagc aaacctcaat acaacagact tttcaaatgt agctaaaggc gattatggat
    15121 tgtggattgc ggaatatggt aggaatttac ctcaaggata tagtcaacct agtccaccac
    15181 gaacaaacaa ctttccagtt gtcgcttgtt ttcaatatac gagtagcggt cgcctatctg
    15241 gttataatgg aaaccttgac ttaaatgtgt tttatggaga tcgagaaaca tgggatttgt
    15301 atgttggtaa aaaatctgat caaattgttc ctcctgaaaa taaaatattt gatgctacaa
    15361 gtgatgagtt tattttcaca cttacaaagg gtagtacaag cgtgttttat tttgacggaa
    15421 aaacgatctt tgaattgtct gatccaatac agctagatca tattcgtgga acttacaatc
    15481 atgtacatgg aaaagaaatt ccatcaatgg tgtggacacc tgaacaattt gatatttact
    15541 taaaaatgta tgataagaaa ccagtatata aataggggtg catagtatga caaatagctt
    15601 aggcgttaaa cttaaagaga aaaacttata ctatagccct aacaatgctt taggttttaa
    15661 ttgcctaatg ttgtttgtaa taggcgcacg tggtataggt aaaacgtatg gatataaaaa
    15721 atttgttgtt aatcgtttta taaaacatgg cgaacaattt atttatttaa gaagattcaa
    15781 aacagaactt aaaaagattc ctcagttttt caagacaatg gcgaaagaat ttcccgaaca
    15841 taaacttgaa gttaaaggga aagagtttta ttgcgatgat aaattaatgg gttgggccgt
    15901 tccgcttagt acatggggta ttgaaaaatc taatgaatat ccagatgttc gtacaatttt
    15961 gtttgatgag tttttgatcg agaaatcaaa aattacctac ttaccaaatg aagcagaagc
    16021 cttgttaaat atgatggaaa ctgtttttcg tgaccgcaca aatactagat gtattctgtt
    16081 gagtaatgca acgagtgtag tcaaccctta tttcttgtat ttcaatctac agccagattt
    16141 gaataagcgt tttaatctat atcaggatcg aggaattttg attgaattgt gtgattcaaa
    16201 agactttgca gaagtaaaga aacaaacacc gttcggaagg ttaatacgtg gtacagatta
    16261 tgaagatttt agtatcaaca atgaatttgt taatgatagt gatacattta ttgagaagag
    16321 aagcaagaat agtagtttct tatgtgctat tgcttttgaa gggaaaatct ttggttattg
    16381 gatagacgct gaaacaggtt gtgtatatgt aagttatgat tatcaaccaa atacaaatca
    16441 tttttatgca atgactacaa aagaccatga agaaaacaga ttgctgatga agaattggcg
    16501 aaataattac tatctttcaa cagtggcgaa agcctttaag aatagttatt taaggtttga
    16561 taatattgtt attaaaaact tgcattatga tttgtttaat cgtatgaaga tatggtagcc
    16621 ctattttagt aaggctacca ataataactc tattacaatg atgaatagta gataacatag
    16681 taattgtaat cttcgatagt tttgttttgg ttctttggcg ttagtgattt ttgctaacgc
    16741 ctttttgttt gcttttggat cgggtgtgtt aatgtatacg aaatcttttc tcatatttct
    16801 ttctccttta aatatccttt tggtatttgt atcgctaact gatagcgaga accaactttt
    16861 acgtaagaag ttaccaattt cattgcctga caatactttt caaggatgtt aaattgactt
    16921 gattcgggca atagtgtcga atgagttaaa aaaagctcgg tgatatttat ttccggaacg
    16981 tcgaaatctt gtaaagtacc ctctatgatc tctatttttt cattgtctga aaggttacgt
    17041 ttacagtaga aacgtaacca ttcaataagt tcgcgatgtt ctttgaatgt tcgtgcaatc
    17101 attttaatca ctcccattta tccgtaattt gtttatatcc gtcatgtttc aattgttccg
    17161 catagtgttc aacgcttttc attgatttcg ttcttgcgat attaatgcaa tggctatcaa
    17221 gataaacata gttatattta tcatgtgtta acacgaactc ttttgtaacg taatcaacgt
    17281 ataaaattaa ttggttccct ccttgtgtta tttctgactt gatagacgct aaactatcgt
    17341 tgtcatcttt agttagttga tttaacccct ctaaaattaa tgataaattg ttaatcatgt
    17401 aaaacactcc ttttatatta atttgatatt gataccacca atcaaatgtg attggtagca
    17461 ttgtattaaa ttaatcagtt atttttgtag ttttccatga atagtcggaa ataagatcca
    17521 tatctaattc ctttaactct tcaaaagata acaaacaata ttcctcatcg cctacatcat
    17581 cgatatctat acgataatgc ttattgtttt cggtatctat gatatgataa ttcatatccc
    17641 agacattaaa agggcgaagt agagatacct ctcctttttc agctattaat gctttgttaa
    17701 acatatgaaa cactcctttt atattaattt gatattgata ccaccaatca aatgtgattg
    17761 gtagcattgt atcgaattaa tacgttgcgc tatcaagata caacgtgtaa tcatcaataa
    17821 ctacctttcc gttttctaat aaaaattcgg cgtcctcctt gtctaaaatt ttgtaaaggt
    17881 aagtttttaa tttatctgta tccgtgaata ggtttgtttt ttctatgtcg ccgtcgtata
    17941 attcaattaa aatatcaaaa tacattttta tcatcctttc tttattacat ctatattata
    18001 tcatgtatct acatatatgt caatcattta attcatttat ttaatgattt atttgattgt
    18061 ttttatgatc ctttctttat tacatctata gtatatcatg catgtttgta tttgtcaaca
    18121 attaaattca tataaatgta gtttggggtc agttaca
//
LOCUS       DANKHJ010000058        17994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000058 DANKHJ010000000
VERSION     DANKHJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            498..665
                     /locus_tag="PW113_002065"
     CDS             498..665
                     /locus_tag="PW113_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526357.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            691..1035
                     /locus_tag="PW113_002066"
     CDS             691..1035
                     /locus_tag="PW113_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2526358.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1040..1321
                     /locus_tag="PW113_002067"
     CDS             1040..1321
                     /locus_tag="PW113_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526359.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1424..1738
                     /locus_tag="PW113_002068"
     CDS             1424..1738
                     /locus_tag="PW113_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2526360.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1716..3410
                     /locus_tag="PW113_002069"
     CDS             1716..3410
                     /locus_tag="PW113_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2526361.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3430..4608
                     /locus_tag="PW113_002070"
     CDS             3430..4608
                     /locus_tag="PW113_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2526362.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4571..5257
                     /locus_tag="PW113_002071"
     CDS             4571..5257
                     /locus_tag="PW113_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2526363.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5257..6417
                     /locus_tag="PW113_002072"
     CDS             5257..6417
                     /locus_tag="PW113_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2526364.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6427..7302
                     /locus_tag="PW113_002073"
     CDS             6427..7302
                     /locus_tag="PW113_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526365.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7299..7610
                     /locus_tag="PW113_002074"
     CDS             7299..7610
                     /locus_tag="PW113_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526366.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7600..7953
                     /locus_tag="PW113_002075"
     CDS             7600..7953
                     /locus_tag="PW113_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2526367.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7943..8344
                     /locus_tag="PW113_002076"
     CDS             7943..8344
                     /locus_tag="PW113_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526368.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8337..8741
                     /locus_tag="PW113_002077"
     CDS             8337..8741
                     /locus_tag="PW113_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526369.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8753..9361
                     /locus_tag="PW113_002078"
     CDS             8753..9361
                     /locus_tag="PW113_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526370.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9381..9743
                     /locus_tag="PW113_002079"
     CDS             9381..9743
                     /locus_tag="PW113_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526371.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9746..9928
                     /locus_tag="PW113_002080"
     CDS             9746..9928
                     /locus_tag="PW113_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526372.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9946..13278
                     /locus_tag="PW113_002081"
     CDS             9946..13278
                     /locus_tag="PW113_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526373.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            13329..14066
                     /locus_tag="PW113_002082"
     CDS             13329..14066
                     /locus_tag="PW113_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526374.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14076..16367
                     /locus_tag="PW113_002083"
     CDS             14076..16367
                     /locus_tag="PW113_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526375.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            16391..>17994
                     /locus_tag="PW113_002084"
     CDS             16391..>17994
                     /locus_tag="PW113_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526376.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEIT"
BASE COUNT         6382 a         2664 c         3791 g         5157 t
ORIGIN      
        1 aactccttct aaattgttct tattatctat catccgttgc tgtctattgt ttttttaatt
       61 attcacacga taactaaagg tgagtgaaga gaaatgattc cattaataat ttcaattttt
      121 gcgctctgtc ttaatgtcta tatgattgga tttaaaaatg ggcaaaataa aaaatagtag
      181 cagccaagaa taattttata gtgtcactgt ggcggaaagg gtagacgctt aaaaataagg
      241 tcaatacgtc gagggatagc cttaacgttt tatgatttga ccatgcaatg ttcgattcat
      301 tgccagtgac tttggcaacc gagggttgga aatgggcgct caaagtacac gagcaaggcg
      361 aggtcgatag taatcgatgg aatcggtgta ggttgcttga tagagctata actgcatctc
      421 gtttatgagg tgtagttttt acagattaga tcactctttg agtggtcttt ttattttaca
      481 caaaggaggt aacaagaatg tatagaccac aatacttaga acagaagtat gaagtaatca
      541 ctgtgcaaaa cggtaacggt gagatagtac gaaagtatag aagaccaata aagagcgata
      601 catacaaacg aaaggaaagc aatgaagtta ttccattgta tggcaaaaga atagctaagc
      661 attaaataag attgcgaaag gagacggaat atgaccgagg aattctatag atggctatta
      721 cagttgataa gagaagatcg tttagttaag ttctatcagt ctcctaaatg gcgcaggctt
      781 agagagaaag cgatgaaacg agatcactat gaatgccaag agtgtagaag actaggtaag
      841 tatcatagag tagaaaacgt tcatcatata aaggaagtca aggatagacc tgacttagct
      901 ttagatttag ataatcttat ttgtttatgt gttgaacatc ataatgaagt tcatggcaga
      961 tatcttacag cattagataa acaagagaag aagatagaaa gctttgctaa cttcgatgca
     1021 agtgaaaggt ggtaagtgca tgatcatcaa tgataatggc agagagtatg atacagaaaa
     1081 gattgaagag tattcatctt atactcaggg attaattaaa cgtttgatat acgttcgcta
     1141 tgtaggtatt agggatctgt tatcagataa ctgttgtagt aaatacaaag tgaatcaagt
     1201 aagagaagcg ttgaataaag ataataacgt cgaaagaata aaaaatgttt ttggatatag
     1261 tattgaagag attaattatt acattgactt cgctgaagct ttcattccga tggtgagata
     1321 accccccctt aaaataaatc gcaaattttt tgggggtgat gaaacggagg gggctgtcag
     1381 gaaaagagat tttttcgaac tttatcatga aaggagggct aaaatgttta aaaacgaatt
     1441 gtctcaaaat cgctacagag aaaaattacg ccgctcttta ataagccaat tggaaagtca
     1501 gaaaacaaat attgagccat tcttagataa tgttgatcgt tatatcagtt tatgggaaac
     1561 ggcgatatca ctggaagaag atatatccga gaacggcatt agactggaga atggtaaaaa
     1621 gaatgaatca gtagcgttgc ttgtttctgt caacaaacaa atgggattga tgttggataa
     1681 acttgccatt actcctgaat tggtaggtga agcaaatgaa tcaattcctg agttataagc
     1741 atattgaaaa ttggttcaaa gctatagaag aaggcactat caaggtatgc aaagagcaat
     1801 tattgctaaa aaattatcta gaagaaagag tctttactag agaagatatt tacttcgata
     1861 agcagatggt agaggattca atcaatatac cagcacaata ctttccattc gaattaattc
     1921 cgtgggaaaa atttctacaa tgttttattt atggtgttcg atggaaaaaa gataaaacac
     1981 tagtgttcaa tagatatctt tcattaatgg gacgtggtaa tggtaaaact ggttttgctt
     2041 cttggaacaa cttctttcta ctaaccgcta aacacggtat taaaaattat gatattgata
     2101 tctatgccaa taatgaaagc caagcaaaga ctagttttga tgatgtattt aaagtaatta
     2161 aagatcatcc tgatttagat aaaaaagtat ttaaagctac gaaggaagtt attcaaaata
     2221 tcgctacaaa cagcaaactt cgttataaca cggcaaatgc tagaacaaaa gatgggaagc
     2281 gaccaggtgc aaaccgcttt gatgaaattc acgaaaatga agattattca atgataaatg
     2341 tggctacttc tggtggtggt aaaattcgag attatagaga attttatgat acaactaatg
     2401 gtcatgttcg tggtggtccg cttgatgaca ttatagaaga atcaaaaatg attctttctg
     2461 gagaacttgg aattgacaag gatggagcag aattttctag tttgtttcca tttatttgtc
     2521 gcttggataa cgataatgaa gttgatgatc ccgacatgtg ggaaaaagct tgtccaacta
     2581 ttaattacaa tgcagattta aaacggaaaa tgtttcaaga atactctcaa atgcaacgta
     2641 atgctggttt aagacttacg ttcatgacca aacgaatgaa cagacctatg gaagatacac
     2701 gatttgctgt tgcttcatat gatgatgttc tgcatacgaa agaaaaagaa tttcctgaaa
     2761 aaatggatga agtgatagga acagtcgatt ttgctgatag acgagatttt gccagcgttg
     2821 ggttgctagg aaaatacgat aaagatgttt attttacaca acatactttt atccacgaat
     2881 cagcccttcg attacaaaac atcaaacgag aggttataga tatttctata gatcaaggaa
     2941 aatcacagat cgttcatgga aaaaatatag aagctgatta tattgtaggt tggtttcttg
     3001 aaatgagtaa taaatattat attaaaaaaa tcgctatgga tatgtaccgt gcaaaaatat
     3061 tgaagcccgc tttagaagaa gcaggtttta ctgtggaaat tgttcgaagc ggatctgtta
     3121 cacatggtat gttaaaagat ctggttgatg acctttttat taatcaacgt ttattttttg
     3181 gtgacgatgc gattatgcgt tggtattgca tgaatgtata tgaagagcat atttctaatg
     3241 gaaatatacg ctatgaaaaa atagaacctg aaactagaaa aacggatggc tttttttcat
     3301 tccttcatgg tttgaatttt ttagatgata tttatgattc tgctcctgta acagtcacaa
     3361 atagctcagt agaaaataca ggaactggat ttactcctct agtattctaa cttgaaagga
     3421 ggtgagaaag tggggatttt tcaaaaggcg gtaggatact tcacaaaaaa agcaacggtt
     3481 cctttagaag aatacttttg taaattgcaa gttgattttg tgtatcgaaa atttgcgatt
     3541 gaaacttgta ttgatttgat tgcaaatgcg atgagtaaag cggaattcaa gtcatatgaa
     3601 gatggaaaaa ataaaaagaa tgatctttac tataggctga atgtagctcc taataagaaa
     3661 aataatgcaa cagaatttag aaaaaaactg atcaggagat taatattcta caatgaagta
     3721 ttgatcgttt ctccgtctaa taattctagc gaaatattta ttgcggatag ttgggatgtc
     3781 acagaatatg cattgaaaga tgatgtgttt tctcaagtgc aaattaacaa catagtcctt
     3841 gatagagaat ttctagaaag tgatgttatc tatataaaat acgcagatca acaaattagg
     3901 caactagtcg atgcgtatta tcaagcgtat gggaaactca tttctagtgc catgaatgtt
     3961 tacaagcgct ctaacgctcg tagatacgta ctgaaaggga atttattccg accgcaagac
     4021 aatacaacac aagatcaaat caataaaatg atgacatcac aatttaaggc ttttatggaa
     4081 gctgataatg caggtgcggt atttcaatta caaaatgagt acacattaga agatttcagc
     4141 ggaaacttcc aaagcaattc aagagatata aaaaacttaa tagacgacat ctttgagatg
     4201 acagcagcag cgtttcacgt tccgaaaaac ctactaaagg gagacatgag tgggttatcg
     4261 gatcaagtgg acgctttttt aatgttcgaa atcataccga ttgctgaact tattcaggat
     4321 gcgtttaacg ctagtctcta tgaagtagaa gaatacttgt cagggaattt tgtacgtgtg
     4381 gatacaacta tgatcaagat tactagcttc aaagatttgg ttgacgctat tgatgtaggc
     4441 attagaaatg gagtatttac aatcaacgaa ggaagagaac gcgttggaaa tgatcgctct
     4501 gataaggcga tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga
     4561 ggtgaggcga atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc
     4621 gcaaattttt attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa
     4681 gggaatcaaa aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa
     4741 ttcaaacggt ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa
     4801 agcaaaagtt aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat
     4861 ggctggcgat actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac
     4921 atacggagaa ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga
     4981 taaatcggta aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga
     5041 acttcttgaa aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga
     5101 tgaaattatc gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga
     5161 agaaattttg aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc
     5221 attaaataaa acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt
     5281 gtaacagctg caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt
     5341 agcgcagcta tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca
     5401 gcaaaaaatc aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa
     5461 gaaacaaaat tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta
     5521 gtgccagcta ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg
     5581 caaaaaatta cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc
     5641 aatccagcat ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatgacttt
     5701 gatgtaatta acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg
     5761 cttgatttag gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg
     5821 agaattgcat tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg
     5881 atgcgtgaca tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt
     5941 acagctttag atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc
     6001 gaaggtgtga atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg
     6061 aatccaactg attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga
     6121 gaatacgtac aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa
     6181 ggaaaagcag ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa
     6241 attgaagctt cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa
     6301 tatgcaaacg gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg
     6361 ggaactacta ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg
     6421 atcagaatga agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa
     6481 gtttgtttaa ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga
     6541 cacgatgatc agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac
     6601 gatgatctgc gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa
     6661 tatttagaag aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcgaatgtc
     6721 attttcaata aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt
     6781 agcgactgct gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat
     6841 attctaaaag atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac
     6901 actaattcag gaggcgcaca gtgggttatt aaccgtccta aagcgaatta ttggaaaaag
     6961 gtttatctgg attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatatacaa
     7021 atctggacag ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct
     7081 aaagttcacg aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa
     7141 gtaaaaatct tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg
     7201 agatacgtga cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc
     7261 tcttacgcat atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg
     7321 aagaattgct aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt
     7381 caattaaaaa gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac
     7441 tttctttttc tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact
     7501 ggaatcgtgc tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc
     7561 aacattatgc gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc
     7621 gaagaaacat tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa
     7681 ctaggaaaaa agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt
     7741 tccatgagag atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa
     7801 gtaaagactc catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta
     7861 atcgatagta tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt
     7921 tatcttgaaa gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa
     7981 taagcaaatt tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca
     8041 agactccgta ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg
     8101 agaaatagaa atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt
     8161 ctattcagaa aatagagaag atttaacagg agactcacta gatattattt cattgattca
     8221 aaatcgttcg attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga
     8281 tcgctatatc gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg
     8341 ctaaaaatag ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac
     8401 gctattcaag cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta
     8461 ttgcagtgga taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc
     8521 accaacacgc aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca
     8581 ttagacctac aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta
     8641 aaagaaatca gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta
     8701 cagaacttct gattcgtcaa ttcgataaat taaataaata gggggaacaa caatggctaa
     8761 aacaaaaact gtagtaacaa cgttcgataa cataagtatc aaacgaattg cttttaattt
     8821 taagaacgca ggaaatgcaa tcgtaacgaa ttgtaatgga caattagatg gcgaaacaga
     8881 aatgcaaacg gtggttaaaa aatgtggagc gacagaagta aaatcaaaat ctaaaccaat
     8941 caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac gtttcaatgg
     9001 gttgaaacaa gatggacgta ttaaaccagg catttactct tacggtcctg attccgtagg
     9061 cgaagatttc tcacttgctg cagagatcgt ggatgacttc gaagaaaata gaaagttagt
     9121 tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga
     9181 tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta
     9241 tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct
     9301 atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta
     9361 aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa
     9421 caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt
     9481 ttaacacaga cgagtttgca aaaatgtcga tgggaaaagg ctctatggat attacagtta
     9541 ttcaaggtgc aaaagccgta tatgtagctt atcgtcaagc gaacatggtc gattacattt
     9601 cattcgatga atttatagat aaatgggatt ttgatatgga ggttgcagta gctgtataca
     9661 gtactatgat gttcaaacaa gcacgtgatg cctatcaaaa agaattcgaa aaagcaaata
     9721 aggaaaaaaa gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt
     9781 ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg
     9841 tttagatgac attttgcaaa tggcttacaa caagagcgct tatgaaggat ggaagaacta
     9901 tgcaataaat caatcccaga aaaactaaag gaaaggaggt aaaaaatggc taaaaagaga
     9961 acggaagcag aagtaacttt catagctaac gatgacggat tgaaatctac gttaaaagaa
    10021 atcagtgctg aattaactaa aaatagagca gaattaaaac tagaacaagc tcaattacaa
    10081 cagactggtt ctgaatcaga caagttagga agtaaattat cttctttaga aaagcagtat
    10141 gaattacaaa gtcaaaaagt tgaagtaact agtcaacgtt tagccaatgc caaaaaatat
    10201 tatggagaaa attccaccga agttcagaaa cttgaaagag aactgattaa tcaacaaaca
    10261 gctcagcaaa ggttatctaa tgatatagat aaaacgagta aggcattggc tcaatctaaa
    10321 ggtgaattta aaacgtatga gtcaactatg aaagacttag ataatgagca acaacatcta
    10381 aagtcaagcg catcattagt agaatccgaa tataaaaaat ggcaagcaac tgctggccag
    10441 tctgctacag aatctgaaaa attagcaaaa gcacaagaat atgtcggcaa acagagtgat
    10501 attgctgaac aaaaaatcga agtgctaaaa aaacaattag atgccacaca aaaagaattt
    10561 ggagaaacat ctaccgaagc tttagaaatg aaaactaagc ttaatgatgc ggaaagagaa
    10621 ttcgaagaat taagtaatgc tgctaaaaac gttgatacct ccacagtaga tgatatcggc
    10681 aaaaaacttg atatgggtaa tttaatggaa gctactgatc acttgtcagt gatcggagat
    10741 aagcttattg atgtaggtag taagtctatt gaagcagctg gaaaagcaca agctatgcag
    10801 gcccaattta aacaagtctt tggctcttta gaaggggaag cacaggacgc cgttgaggga
    10861 atggctgaag aatttggaat gttaccaaat acgatcaagc ctgtttttat acaatatacg
    10921 tcaatgttta aaggacttgg atatgatacc aaagaagcta tggagttagc tggtgatagt
    10981 actcagttag cagcagatgc agcagctttt tacgataagt ctatggatga tgctagtgaa
    11041 tctcttaatt catttataaa agggaactac gaaggtgggg agcaaatagg tttatttgct
    11101 aatgatactc aaatggcagc ttatgctgtt aagcataatt tgataccagc gactgaagga
    11161 gcaaaaaaag ccagtgaaga gtcattgtta gctgttgaaa aagcacaatc taagtatgct
    11221 gatgctatta agaaacatgg tgaaggatct ttagaagcaa gagaggctgc tttaaaactt
    11281 aaagatgcgc aagataaaat aaatgaagaa ttaggcccac aaacgcaaaa atggtctgat
    11341 ttagatgagg ctaccaagca agcagttcga gttcaatatg ctgaagatat gcaaaaatta
    11401 gccggtgcta caggtcaggc tagtagggag tctgatggtt tagaaaacca aatgacaaga
    11461 gcaaagcaag cgttagaaga tttttatgcc tcattgggtg aagatatact gcctgtgttt
    11521 atcaaagggt tgcaagcagg agcgaaagct ttgcaaggat tagccgaatg gtggagtaaa
    11581 cttgatgggc caatgaaaaa tttcatttta gctcttggag gaattctagc attattaagc
    11641 acattagctc ctgttataac cgcagttgtt acgatagttg gcacatttgg ttctacagtt
    11701 ttgctgccaa taataggaat tatagcgggc gttgcagctg taatagcgat tgtaattaca
    11761 gcgtttcaaa actggggcgc aatcactgac tggtttagtg atttatggaa aaaatttacc
    11821 gattggttag gtgatacctg ggaaagtata aaagaaagtg cctcatcagt ttgggatgga
    11881 gtcaaagaaa cctggtctgg atttgtagat tgggttcaag aaatttggca aggagtttct
    11941 gattggtttg gagagttatg gagcggatta gttgaaggag cttccaacat ctggcaagga
    12001 gtccaagaga cttggcaagc attcgttgat tgggtttcaa atatttggaa cggagtcaaa
    12061 gaagtatggt cgattatttg ggcagacatt gtaggaattg ttcaaatacc atggacatta
    12121 ataacgtcat tgattcaagc tggtattaat attatcgtgg gtatctttga tgtagctgga
    12181 cagttattag gcgcagcttg gcaagctgtt tggacaccta tttctgattt ccttaaaaat
    12241 acttgggata ctatgacaca atggataagc atcgcttgga atggaattgt aactacattc
    12301 catactatat ttgatccagt agtggcatgg tggaatggta tatggacatc tattagtact
    12361 acggcttcaa atatttggaa ttcaattagt gcaacagctt ctagtatttg gaacagtatc
    12421 aagaatacaa tcactagctt ggtacaagca gctgctacag taattcaaaa tatttggtca
    12481 actgtatcta gttggttagg tggaatttgg aattcaatca gctctacagc atcaaatatc
    12541 tggaatagtg tgactagtag tataagcaat gctataaacg cagctaaaag tgccattcaa
    12601 agtgtttgga atagtatatc ttcgtggatc agcggaattt ggaacggtat caaaaatact
    12661 gctttgaatc tttggaatgg aattacaagc actattagct ctaaagtaaa cgatggaaaa
    12721 aatgcaattt caagcggttg gtccaatcta acaggtattg tttccgacat attcaataat
    12781 gttaaaagta caattgctaa catatgggaa ggcatcaaaa agactgttag cgctccaatt
    12841 gattggatta gagacaaaat cagtggcatt tttgataatt tgaatatttc tataccacat
    12901 attccgttac cggaatttat catggaaggc agctttaacc cgctaaaagg tcaaatcccc
    12961 catttgcgtg taaaatggca tgctaaagga ggtatcttta ctaaaccaac tttactaggt
    13021 ggaatgaacg gtgtcggtga agcaggacct gaagcagttt tacctttgaa aagatctgtt
    13081 ttgcaagaaa ttggtgatcg tatcttgagt agcacatcag tttcatctag ggcacaaacg
    13141 attcaacctg tgaataacta cgaattcaat ttcacaattg atggtaacgc agatgaggtt
    13201 actatgaagc aaacaactca acaaatcatt gatagcatta caaaaattca aaatgataat
    13261 gcttcggcat ggcgttaaac aggagagtat ttctcctgtt tttttagtat taaaaaggat
    13321 gtgaaaaaat gactgattgt atacattcta taatcgatgg atttcctgat tatttgcata
    13381 aattggcttt agctgaaaga ccaaccatac cttctccaaa aagacagaga gttgaaactt
    13441 ctgttttagg taggttaggt ggcttagtac aagattactc gtttgaagat atgtcgttta
    13501 cattgcacta taactattta gaggatgtgg aagaccatca agcgttcaag caatcgtttt
    13561 atatcatgcg tcattggtta aactatgcaa agaaattaga attctctgac gatcccaacg
    13621 tctattacgt tatccagact atcgatattg gggatgcaga aaacgatatt gttgaatggg
    13681 gagagttcga tgtaaatatt actgcgaaac cattcgcaag agttcaagaa gatgtaccta
    13741 taaccgtaga taaaccacag tcatttaact tgctgaataa tagtttagaa gaaagttttc
    13801 caaagattat catcactcct tcagctactt catgccagtt catcttaaat gattatgtgt
    13861 ttagtttcga aggcttagta gcaggaactg atgtagtcat tgatagtgat ttgatgcttt
    13921 gctacgaaga gcaatcggac ggagatattt tagatcggtc caacaaaatg aagaccatgc
    13981 aatatccaac gttgcaagtg gatattaatc attttaattg tactggtttg agcaaaatac
    14041 aaatttatcg taacgggtta aggtaggtga aatagatgat cgataattta ataactattt
    14101 acgataaaaa tgacgcgaat aatttagctg aacatttata tgatacgcaa ggtttaggcg
    14161 ctttgtcaga ctggttaaca gctactgtta gcaataaact aaacggagcc gagatatttc
    14221 agggtactta ttcaataaac ggaactaatg cagatttgat tgtagaagga cgtattattc
    14281 agtgttatgt agatgaaaat cgagcaaaac agcgtctacg gatttattat gcaaaaactt
    14341 ctgtaatagg caatacgata gaagtaaaag ctgaacctat tttcaatgat ataagaaaat
    14401 cggtgttgaa taaatatgac agcggaacag aaaagatcac tgctactcag gcatggcaaa
    14461 acgcaaaagt tttagcgaaa ccagctatcc cttcgcagtt ttctttctcg tcattagtag
    14521 atacgcttgc taatgtgaag atagaaaagg cgaatttttt agaattcttt ggtggaaaag
    14581 agggatctat tctagatcga tttcatggtg agtttctaaa agataataac acattacgtc
    14641 atgaaaaaag tctaggtacg gatcataaaa tcaaagcgat ttatactaaa aacttaactg
    14701 gtcttgactt agagattgat gctcaaagtg ttttagttgg agtttatcca ttcattagca
    14761 gctcttcaga aggagaagac gagatcactc taccagaaga agttattttc acggattacg
    14821 tggatgatta tcctgctgga tatgtttctt ttgttgattt taaagacaaa gcgactgatg
    14881 tagccacatt aagggaagct gctaaagact ggttgaaaac aaacatagat aaacaaaaac
    14941 cacaagtgag tggttcgatt gaattagtac cattgaggca tcaaagaggc tatgaaaaat
    15001 ttgttgatct agaaaaagtt tcgatgggtg acggagtaga tgtgtatcat ccacagttaa
    15061 aagtgaatat gtcagcgaga attgtggaat atacgtttaa tgttctaacc aattcatacg
    15121 ataaattagt tgtaggaaac gtcaaaacaa acttcttaga aaacacagag aataatgtca
    15181 gcaatttgat taatgatgcc attgatcaat tgaaaaatgg tggcgaaatc agtgatttaa
    15241 tcaatgatat tgtagatcat caaactgata tgattactgg tcaaaatggt ggttacgttt
    15301 tattagatcc taaagaagcc cctagtcgta ttttgattat ggacacacca gataagaata
    15361 ctgcacggaa cgttttgcaa atcaacaacg ctggtattgg tttctctaaa actggcatta
    15421 atggaacgta tgacaccgca tggacgttag atggcggatt caatgcctcg tttattacag
    15481 ctggtgagat agtagggatt actattagag gtactacatt aattagtgat ggcactgatt
    15541 atagaacaag tatagctaat ggcaaaatga cttggtactc aaaaaaagtt aacaaagata
    15601 ttatggagct agaagcacgt gattatgtaa gtgctgatgc cggcattgta tcatacacca
    15661 tgaaaactgg tggtggtttc atgattagaa atccacaggg taacttggtt tttagtacgt
    15721 gggataatgg taataacaga ccgtttttat cttttggtgc gccaaatttc agatacagta
    15781 atgctagtta tataaatgat ggtgacggta attctttagg tattgatggt agtgcaggta
    15841 attcatggga atttaaagta gctggtagga ctatgaaatt tactagtgac ggtatgctaa
    15901 cgttgccagg ttgttttttt ggttcatggg aagatgggaa acttgctaga tttgaacaat
    15961 caacggtaca agtatataaa gattttactg ttagaggtac taaaaactca accgtaccaa
    16021 cagaacatta tggacaacga ctattgaacg cttatgaaac tccagaatat tatttcgctg
    16081 attatgggga agccgttaca ggtgatgatg gtaaagttcg tgttgatatt gaccccatgt
    16141 ttgctgagac agtaaatcta agtcggtata tgacacatgt gacacctaca gaactagttt
    16201 tgtgtgctgt tactcatgaa gatgttgacc atttcatcat tgaaactagt aagccaaacg
    16261 tattggttag atggaattta gtggcacatc gactagggta tgaagatgtt agattaaaag
    16321 aggatacagc atatgatagc acagtgcttg accaaaaacg tttttaaaac gaagacaagg
    16381 aggtatataa atggctagca gtttatataa tttggcttta gatttcagca aagaattaaa
    16441 ctacaccaaa gctattatgg ctcgtcaagg tgataaaggg atcacggtga ctgttaaacc
    16501 gtttttaaat ggcttgcaga tggatacgag cggcggaaca tttactttaa aaggaactac
    16561 gccatctaac cgttacgtag atagtgtcgc tactagtgta actagtgaag aagtcacatt
    16621 ttctcttgat ggcacattta tgagtgaagc agggtattat aaacactgct atgtagaata
    16681 tagaaaagac gatcaaattt taacgacgca agatatcatt tttttctcac taggagtgtc
    16741 tgacatttcg caaggccaag ccgatgaata tgtttcgcaa ttagaagaat tgattcgaaa
    16801 gtataacgaa acttttgatg cttttctggc tgaaattaaa ggtagaatgg atagcttaaa
    16861 taaacagatt actgatttaa ctggtcaagc taaaacacta caagacaagt tagatgctct
    16921 gaaagaagaa atttctaaat taggtaactt acatgtgatg tactccaaca gcatcgactt
    16981 cgggggctat gattatagtg ggaatccgaa tttaatgtcc aaactaaaat cgagcgattt
    17041 taacgttggt taccacgggt cactaactaa tgttaacgga atgttacatt ttatgtctga
    17101 tggtacaggg gacattgata tgtttacgcg tattaataca cctcagcttg ctagtggcaa
    17161 aacatatact ctgagtgcaa aagttcgatt tgatgaagga actacaggag ctattaataa
    17221 attacgtttg gtatatcgta catcaccagg agaaaatata ttattggaag caaataatac
    17281 aactattaca atagatgatg tagggaaaga aataacaatc aaaggcacag ctaacgttaa
    17341 ttatcaaatc acaaatttag atcgttttta tattagtgtt agtttttcta acagggataa
    17401 aataaatggt ggatttaaat tgtacgacat caaaatcgaa gaaggctcaa cagccactcc
    17461 gtatcagcca aacttactag atgaccctta ctgggtaggt aaaacatcgt tgggtaagaa
    17521 tattgcaaat aaaggtacga cgttcccaat taaatctagc gcgtacgaaa tatataaagg
    17581 taacacggaa gaagagctta taataggtca aacgtatact atcacgctta aaggaacaaa
    17641 acccgcaagt caaacctttg tagcatataa ttattggaac gttaattttg gagatctaaa
    17701 accagttgag ggattgacag acgtatggtc tctaacattc acaccaacga aacttgaacc
    17761 gggtttgcct aaagaccttc gcatttttca gtcgcctaaa gaaacggccg gcgcatgccg
    17821 aattgactgg ctcaagatcg aaaaaggcga cacacgaacc ccgaatattg agcaatataa
    17881 ataccgagga atcggcatgc gagactcaaa caatccaaaa gattatgtat gggatctaga
    17941 acctagctat gttgagaata acctagctac agatatcaag gttgctgaaa tcac
//
LOCUS       DANKHJ010000059        17699 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000059 DANKHJ010000000
VERSION     DANKHJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            131..517
                     /locus_tag="PW113_002085"
     CDS             131..517
                     /locus_tag="PW113_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526377.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            514..717
                     /locus_tag="PW113_002086"
     CDS             514..717
                     /locus_tag="PW113_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2526378.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            714..1010
                     /locus_tag="PW113_002087"
     CDS             714..1010
                     /locus_tag="PW113_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526379.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            1086..1502
                     /locus_tag="PW113_002088"
     CDS             1086..1502
                     /locus_tag="PW113_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2526380.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            1763..1900
                     /locus_tag="PW113_002089"
     CDS             1763..1900
                     /locus_tag="PW113_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526381.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            2310..2540
                     /locus_tag="PW113_002090"
     CDS             2310..2540
                     /locus_tag="PW113_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526382.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            2699..3532
                     /locus_tag="PW113_002091"
     CDS             2699..3532
                     /locus_tag="PW113_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2526383.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            3525..4940
                     /locus_tag="PW113_002092"
     CDS             3525..4940
                     /locus_tag="PW113_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL2526384.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            4952..6481
                     /locus_tag="PW113_002093"
     CDS             4952..6481
                     /locus_tag="PW113_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2526385.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            6399..7451
                     /locus_tag="PW113_002094"
     CDS             6399..7451
                     /locus_tag="PW113_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL2526386.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            7453..7770
                     /locus_tag="PW113_002095"
     CDS             7453..7770
                     /locus_tag="PW113_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526387.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            7897..8520
                     /locus_tag="PW113_002096"
     CDS             7897..8520
                     /locus_tag="PW113_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL2526388.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            8535..9425
                     /locus_tag="PW113_002097"
     CDS             8535..9425
                     /locus_tag="PW113_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL2526389.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            9448..9663
                     /locus_tag="PW113_002098"
     CDS             9448..9663
                     /locus_tag="PW113_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526390.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            9675..10016
                     /locus_tag="PW113_002099"
     CDS             9675..10016
                     /locus_tag="PW113_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526391.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            10016..10387
                     /locus_tag="PW113_002100"
     CDS             10016..10387
                     /locus_tag="PW113_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526392.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            10380..10775
                     /locus_tag="PW113_002101"
     CDS             10380..10775
                     /locus_tag="PW113_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL2526393.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            10777..11154
                     /locus_tag="PW113_002102"
     CDS             10777..11154
                     /locus_tag="PW113_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526394.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            11155..11763
                     /locus_tag="PW113_002103"
     CDS             11155..11763
                     /locus_tag="PW113_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526395.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            11763..12104
                     /locus_tag="PW113_002104"
     CDS             11763..12104
                     /locus_tag="PW113_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526396.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            12346..15135
                     /locus_tag="PW113_002105"
                     /pseudo
     CDS             12346..15135
                     /locus_tag="PW113_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            15125..15865
                     /locus_tag="PW113_002106"
     CDS             15125..15865
                     /locus_tag="PW113_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526397.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            15862..>17699
                     /locus_tag="PW113_002107"
     CDS             15862..>17699
                     /locus_tag="PW113_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2526398.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         6508 a         2396 c         3597 g         5198 t
ORIGIN      
        1 attttttaag cagattaccg acattagcat tcagcaagga atggaagacg aagatggaaa
       61 ctttatcgat gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg
      121 aggcagaagg atgaaaaaat tgcaccaact catatctgag aaagaaagcg aactccaaaa
      181 tttagaagat agtttgggat tggggtttcc aattgttgag caagctaaaa tgactcaaat
      241 cagtcatcta cgacttgaat tggaagattt aagacaaata gaaaaatcaa ttcaactcaa
      301 cgacaaccag aaaatcgtgt tggagtggtt gaaaagcgag acaattttaa ctagagaagc
      361 accaatatta tctgttaatg ctttttctga taaaaattta ctaggaaaat tacctgataa
      421 agtacgcaaa gcttataaac tattggcttg taaacaagaa tatgaagtcc tatcagcatt
      481 cgctcaatgg ggattagaac aggaggaagc ggaatgaaca acagacatcg cagaataaca
      541 aaactaagaa aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt
      601 tcagcagaag aaacatataa agtggcaagt cagtttgttg ctgatgcgag taatgctatt
      661 cgtaagtttg ggatttcgat attaaatgat gatcgtaaat gggaggaaat gagatgaaaa
      721 taaaagacgg attttacgct agtagtcatg gtatcggcgg tttaatgcta gacatgccga
      781 taaagaaccc taaaacacgt cagaaatcaa aattcaaaat cggtgacatg gtccgatgta
      841 aagcagaaga gttcatctat ccgtttcgtg gatatgtaga gcatgtctat aatcattcag
      901 caatcattca cattgaaaac acgatggaat gcgataagtg gttagcgaaa agcaaagata
      961 atttagcagt ggctcgtttg gtggatatgg aactaatcaa tgacaaataa aaaagccgga
     1021 tcgctccgac tgattcaata aatccaacac atttattata tcacataaag gagcggtttg
     1081 acttgatgca attgttacga gaggtagatt tcaaacagac aagatgtaat gcgagagatg
     1141 tgctgaagaa ctttcggcgt ttggagcgga tggcaggtcg ctctttgata gatattaagt
     1201 cgccgattat tacggatatg ccgaaggcac cgaagcacgg caataaggca gaagacgcga
     1261 tcattcagat gatggatata gaagcggaga gagacgcaat actagcggct ttgatggctc
     1321 ttagtctgat tagccgtcag atactctact acagcttctg tgacgtaaac aagcactcta
     1381 attatgaaat agggcaattg atacgaggat acggagagaa gaatgtagag aagttgaaat
     1441 ccatcgcatt aatcgaattt gcagaagcat acaaaaaagg cgtgttagtt cagtatcgtt
     1501 gattttgtag ggtttttgta gggatagtgt agggtttttg agcggtttaa cgtgatatta
     1561 tggtagtgtc gaaagattag tgataggtct gagacaaaat aaaatgtaag ggaggaaatc
     1621 tccctcatcg ttttaactta agcttcgata gacagcaacg gaaatattaa gaataaggat
     1681 gtgaatttca actccttcta aattgttctt attatctatc atccgttgtt gtctattaat
     1741 ttatgcatcg gaggaaaaac gaatggataa agaaatcaaa gtaactgtca aattagattt
     1801 gactgaacta aaagaactgc tcaacaaggc tagtgaccaa gtcgaacagt tacaagaaac
     1861 tttagatgaa attgctaatt ttaaaatcca agtttcttag cagtatattt tttagcagct
     1921 gcactcataa aatcatacca agtatcagaa aaatataact taactaaata attaaaaaag
     1981 gtgggtaaat agaaatgacg gcgttaataa tttcaatttt tgcgctctgt cttaatgtct
     2041 atatgattgg atttaaaaat ggacaaaata aaaaatagta gcagccgaga ataattttat
     2101 agtgtcactg tggcggaaat agaagacgca aaggtggagg taggaagcgt atggcgcact
     2161 atcgggacct tgtacaaaac tagaaaggac tagtacgtgt gtggtgcgag ccctagggag
     2221 gaacaggata accgcctgtg tgtaggttgc tattacatat tagatcactc tttgagtggt
     2281 ctttttattt acataaagga ggctacataa tgagaaacta ctggtatata tcgctaacta
     2341 atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct gtccaaatca
     2401 aaaagaagta ctccattgtt gaaatgacca gagaagctac gccaaaagag attgataaat
     2461 gcaaacttct ttattgcggt catggtttct atgatgaacc aaacattcaa aataatatta
     2521 acaagaattt gagggattag aatgcaagaa atggcttaaa aatctccaat caggacaaat
     2581 caagttagac aaagtttccg atttaaagat attgattgaa gctgatttaa tgctaaaaga
     2641 tattgaaaat tagaaaacaa aactcaacct aagaagattg cgaggtggtg tgtattgaat
     2701 ggcaagacaa cgtgatccaa gacgtgatga agccaaaaga atttggttag aatccaacgg
     2761 agaaaagcag ttaaaggaaa ttgcatctga attaaatgtt tcagattctc aggttagaaa
     2821 atggaaatcg caagacaaat ggagcgctga attgaaaagt aacgttacca atggcaaaag
     2881 taacgttact aatcaaggtg gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg
     2941 aaacagcaga gcatcaccgc caaagagaaa taagaatgct cttaaaactg gtgagtatga
     3001 aacaatattt tttgacacgt taaccgataa tgagaaggac atctattcta gtttgaatga
     3061 tgatccttct tttgctttgt ccgaagaaat acgacttctt aaaataagac aacttcgaat
     3121 gatgaaaaga atccaacaag ctgaagctgg actaaatgat gaagaagtcg aacgattgca
     3181 gcagctaaga aagattaaaa ctccgattga aaaaaatggt aaaaagctag aaatcaagcg
     3241 tgaggttatg caagatgtgc agattagcag aaaaaaacat cgcaaaattg atgatattct
     3301 ttcaattgaa gattcattga ctcggattag caaccagtta gctaaagcca tcaagcaaat
     3361 gaatgaactt tatatgaatg aatacagaac tgatttaatt aaagctcaga ctgataagat
     3421 ccaagctgag acaaatgaaa ttggcggaaa taattcaggt gaagaaatag aagaatggaa
     3481 acaggcagtt ttaaatgccg caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta
     3541 ttccttttgc tgatattggt tctgccattg attattacta tgataaacca gtagcttttt
     3601 gccaagatat tttgcatttg aatcctgatg aatggcaaga aaatgtttta aatgatttag
     3661 ctgaattttc aaaggtttct gttcgttctg gtcaaggagt tggaaaaaca gcattagaag
     3721 caggagcaat actttggttc ttaacgtgtc gaccctacgc taaagtaata gcaacagctc
     3781 cgacaatgaa gcaactttac gatgtacttt gggcagaggt agctaaatgg ttaaatgata
     3841 gcttgatcaa aaacttactg aagtggacaa agaccaaaat ttatatggtt ggtgattcag
     3901 agcgttggtt tgctacggct agaacagcga ctaaaccaga aaatatgcaa ggttttcacg
     3961 aggaccatat gttgattgtg gtagatgaag cttctggtgt gtctgatcca attatggaag
     4021 ctattcttgg tacgctatca ggttttgata ataagctgtt gatgtgtgga aaccctaata
     4081 atattgaagg tgtcttttac gattcccaca attcagaccg tgataaatac agagttcata
     4141 aagtatcaag ctatgatagt aaacgtacaa acaaagacaa tatagaaatg attcttaaaa
     4201 aatatggaaa agaaagtgat gttgctcgtg tccgtatttt tggagaattt cccaaaggtg
     4261 cgttggattc atttatcagt cttgaaacgg ttgaattggc tacagaaaaa caaattagtg
     4321 attctttagt caataaaaca acggttgctc atattggtgt tgacgtagct cgatatggtg
     4381 atgattctac gattctcttt cctagaattg ctaccagggc attggagtat gagaagtatt
     4441 caaaacgtag cactatggaa acaacaggat atgtcatcaa catggccaag aatctaatga
     4501 gtcaatatcc gagtattgat aaagtgatga ttaaagtcga tgacactggt gtcggaggtg
     4561 gtgtaaccga ccgcctagaa gaacttatag aagacaagca ttatcctttt gaggtgtttg
     4621 gagtgaataa cggttcaaca tcagaagacg atttttacga taatttaggt actcaactat
     4681 ggggaaacat caaggaaatg ttagaagaaa atatgacagc aaatcttaac ggagaacagc
     4741 ctgttattga attgccttct gatagttcgt taatcaaaga attaagtact cgcaaattca
     4801 aaatgacaag tagaagtcgt atacgtttag aaagtaaaga tgatatgaaa aagcgaaata
     4861 ttggtagtcc cgatattgct gacgcactgg ctttagcgtt ttatgagcca ccaagtcact
     4921 atcaatttat tcaattttag gaggtgagtt tttgattagt acagtattat ctttagagag
     4981 atataagaag ctacgtgtta aatatgcaac acaaattgaa gatggaatgt ttgatccgaa
     5041 tggttttata gaatatatga aaccattttt tgctgatcgc gaaagaaaat atctagctta
     5101 tactagcgaa aagaatgaaa tagacaatag accaaagcct aacactgata ttgtaaaagt
     5161 taataataaa cttcatgctg gtatgtattc aattgtcgta gaccaagcag tcaatcattt
     5221 tactggtata cctatcaaat gggattacga tgtatcggaa caaaaaagaa cactcattca
     5281 aagattaaaa gataaattct taaaaaatga tatagaactt ccaacagttc cagaagcttt
     5341 taacaaatta acaagcaatc ttgattccat gagattcgca atgcttgatt ctgaaactgc
     5401 aacttttcaa ggagcttgtg gagtagcttt ccgattgtta gagcctgtgg aagaagatga
     5461 tggttggaag ttaagagcaa gtaatattga accttggaga gcagaaagat atggaaatgc
     5521 cggaatctat atcaaagaaa agtatgactc ttaccagaaa aaattttttc aagaaatgaa
     5581 agttatgaca agaaataaaa ttctgacata tgcttgctat ggtgatttga attttgttac
     5641 aagcggaacg ttcaaaaaaa ttgatgagac cgataaccct ttggggacga ttaccttggc
     5701 agaatttaaa aataatacga atcgttattg tgattttgaa gtagctgagg aaattggaga
     5761 tgcaattgat cgtgctttgt ctgatcaaca aaacgaaatc gaacaattta agcttgcgta
     5821 catgcttgtt actggcacaa caatgagcaa aggcactgct aaagaaatga tgaatcagct
     5881 agggattatc aacttaaaag accctactgc aaaagctgaa tatgtcacaa aaaaattagc
     5941 aaaagatttc aatgaatatc atatggatct attgaaaaaa cagttttaca ccatttgtaa
     6001 agcaatcgat tttaacgatg aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg
     6061 gcaaatcatt agcctagaag cgaaaacaaa tactaaagag caatatttta gagaaggatt
     6121 gaaagaatgc gcagaaacaa ttgctgcttt tcttaagttt catgacaaag tagaaattga
     6181 gcctgaaaaa attattttta ctttctctag atctttgcca actgatttaa gttatttagc
     6241 tgaagcttta cctaaacttg caccatacgt atccaaacga actattcaga gccaaattcc
     6301 atttgtaaca gatgttgatt atgaaaatga aatgatggaa ttggaaagtg gaagtgctta
     6361 tccagatagt gaatacaatt ttggcggagg tggcaatagt gacgatagaa acgaagtatt
     6421 ggaccaaacg tcgagaactg gaggatcaag caaggctcaa acaagaaaat cagacactta
     6481 aaaaattaac tagtgtattt cctgaagcac tgaaagagat acaagcaaaa ctattatcac
     6541 aagctgactt acacaatatc acttatccag aaatgatgga gttttacagt acaagtaatc
     6601 agaaaaaata tcgtgaatat gtggaaaaaa attataagtc attaaaaatg tatgatgcaa
     6661 aatacaaaga gtttatcgat gaattttttc caccatttga ctatgcaaaa gtcaatcgct
     6721 tattacaaat acgatcagat gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg
     6781 tgaatcaata tttttctgat cgcttagagg aaattcttca aagaacatat tcttctaatg
     6841 ctaatgtttt tgttcaactt ttaaacgttg atataccaaa ttatttacca gaaaatgaaa
     6901 ttaaacatta tttgaactat ccatggtcag gaaagacatt ttcaagaaga ctttggggaa
     6961 atatttcatc gcttgaacag aaactctcta atgctattgt aaaaagtgtt gctagtggag
     7021 aaggcgttat acacgcatta aacaccatga gattagactc agaaatttgt gacatgttta
     7081 agttagaaga atcaaagtat aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa
     7141 aatttgcaca agatggtatt gaaaaatcat atttagaaac aggtattgag gaatacaacg
     7201 tattgactgc aaaagatgag agagtttgcc ggatttgtgg aggaaaggca agtaagaatc
     7261 cctataaact gaaagatgct gtcataggtg aaaatcgagc acctttccat agtcgttgta
     7321 gatgtacgga tgttcctaat ttaccaaaat taggaaagga tattgatgaa gaatatgacc
     7381 gtttatttgg cgatttatta gatgagtttg cacatgattc ttttggaatt aatttgaaac
     7441 ggaggaagta gaatgaaaga tttttttgaa gcagtactaa caattaatgt aaatgctgat
     7501 attgcagaag cctacaaaac agctattgag tctgagaacc atcctaatgg cttgagagac
     7561 cattggaatg gcaattatgc ctacgtggtt attggcgatc aaactgttaa ttatcaagat
     7621 aatactccag ttgataagaa taccgttaat ttaacgattc aattattatc tcattcatta
     7681 ccaaatttaa aagaaacagt tgattggtat gaaaagatgg gatgtattgt tgttagaact
     7741 gactacaaag aaggaaagtc tagtaattag gcttttttat tttgtccgaa atgacgttaa
     7801 actagcgcaa tactgggctt ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat
     7861 gcggggcgtg aaaacgaatc gtggggcgaa aggagaatga gcatgaaaca caaatcatta
     7921 atgccaatga atttgcaata ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac
     7981 ttcaaatctt ttgtggaatc caatgaagaa gctcaaaaat ttattcagtc acaatctcaa
     8041 agtgttgccg acaaacaatt ggaagcttgg aaacaaaata acttagataa aattaaacag
     8101 gataccatca aggaatatga ggaatctaag aaaaacaagt cacctgagca aattcaactg
     8161 gaaaaactac aagctgaatt tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa
     8221 gcatttgttg cagaacaaat tgctggatta gaactagatg gagagttaaa agagtctatt
     8281 tctcaattta tgctaaataa tcttgttagt tcggatacag atttcactaa gaatgctgtt
     8341 gaaggtttta caagtgtttt gaatgcaatt aatgagaaac atgcagatgc attaaaagaa
     8401 ctacaaatga agtctgcatt tggtggaact caacaatcga ataaccaggt tcagcagaac
     8461 aatgaaacat taacaaatcc agaagaacaa ttaggacaaa ttcttcaaca atttaactag
     8521 gagagtgaaa aattatgaaa aaaacatctt taaataattt agagtatttg gatatttcac
     8581 cagcgattaa tgctatgcaa gtaccaaata cacctttttt aagctattta cttggtgctg
     8641 gaaaaacaga gccagcaaac tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg
     8701 atgattcttc tgaaaaactt gagggcggag aatatccaga tgctgaatca ggtcgaactt
     8761 ggtttaacaa ctatactgaa atttttagaa aatcaacctc tgtatctggt acattagatg
     8821 ctattaatgt gaatggagtc ggaaatgaat taactaatca agtagcacta cgtggtatgg
     8881 aaatgaaaat tgatttgaac cgaaaattga ttactggtgt aaaagctgat gaaaatggtt
     8941 ctaaaggtcg tcgaatgaat gggattttga acttgatcaa ttcagcaaat aaggcagaaa
     9001 cagccactgc gggtgcagta acaagaaaag atatcgatac tttatttaaa ttgatgtatg
     9061 aaaaaggtta tatgggagaa aaactatgct tgatttctcc agatatgcag gagttaatga
     9121 ctgatgagtt agatggaaaa tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg
     9181 gattgcaaat tggaaatatc gtgtctaatt acggtacagg tattgctcta ctagaaccat
     9241 cattgccaaa aggaacaatt gccgcaatcg atactaatta tgtgaaacta cgtccattac
     9301 gtgaatggag agcagaagaa cttgcaaaaa caacggattc tagacgtatc ggtcttgtag
     9361 gtgaatactc tcttgaatac aatgcttcaa actctggggc aattttaaat ttaaagtctg
     9421 aataaaaggg agttagtact cccttttttg ataggaggaa ttacagtggt aaaaaaagat
     9481 gaaactaaaa aagatgaagt cgtgaaatat agagtaggta aaactaaaaa ttttgttggg
     9541 tttgttcatc ctaaaactcg tagatttatc acagcagatt caaataacga atttatcatt
     9601 tctatagatg ataaagaagc aattgcaatt ttggaagatg caattgatgt taatgaaatt
     9661 taggaagtga tctgatggat gaatcgctaa aaacggaaat cattgagtct acaaaagaag
     9721 attttccaga tttgagtgaa gaacgcataa ctaatttatt agaaataatt ttgctagaaa
     9781 ttgaatcata caacacttgt aaaaatgata tttcatggga aaagttaaaa agcgtgatta
     9841 gtgaagtgtt gtataaaata ataaaaaatg aatcagaaaa aacagtatct tcagttagac
     9901 gtggcgatac aacgattagt tatgcttcaa caacaaatga tgttagtgag ttacttctag
     9961 gctatggtga tttgatacga agagttattg gttgtggagg attggagttt ttttaatgaa
    10021 tgaagcagat attttagaaa ctacttatga agatagttgt attattgaaa gactgacgga
    10081 cattgaagat tctaatacaa atattactat tcaagattac aaaaaagtat atgataatcc
    10141 tatttcttgc gctctttcac aaggtcaaat cgatggacta gcagtcatag aagatggaga
    10201 gatggtaaat gtttcaactg acacatataa attatttgtt catcctaaga ttaaactaaa
    10261 aaaaggagat cgaataacaa taactcaaaa ggccagtggc ttaatttttt ctctatttgc
    10321 tactaagcct ttttactatc ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa
    10381 tgggtaatct caaatatgaa tcaaatgcag aaaagatcat tgagaatttt aaaaatatga
    10441 ctgtaattgc tcaaaaagaa ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta
    10501 gtttagctaa accattaact cctgtaaaat caggtaattt aaggcgagga tatcgtgtag
    10561 taaaagctag aaaactatca agcggtcgaa ttgttggagc agttataaac aatgaacatt
    10621 attttaaata tgtagaagaa ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat
    10681 tcatgttaac tcgtgcaacg aatcttgcaa atatgtctta tattcctcga agatttaaac
    10741 aaatggcaat aaaaatcgtt aagaaaggaa agtaacatga aagatgaaat cattgctgca
    10801 atcagtagca aattaaaaga aatctatcct gatgggacaa tatatcttga ttcagttatg
    10861 cagtcaacta aagattttta tttcgtttta tcagtaatgg aatctggaac tgaaaatgta
    10921 ggaattgata ttcaaaatgt ttctttctta attgatattg cattgattga taataaacct
    10981 aatagaaatt taattaatga attagtctca cgttgtggga ctttttttaa tacgattaca
    11041 atcgatgagc aaatattatt tccaaaagcc tatttacctg atgaagcaga tggtgttcaa
    11101 catattcgtt ttacattaga atttccacaa tatattgaat ggagtgaaag ataaatggga
    11161 gaaaaaagaa gtaaaaccgg aattatttct gtcgaaaaac ctacttggtt tcctttggaa
    11221 gatgagacag ggaattttcc ggtatatggt actgcgttgc caatgggaac agcagtaagt
    11281 atcaaaccaa cagctaatta tgaaacaaca caagattatg gggattcagt ggttcaagat
    11341 caatttacag cttttggtgg tgcagaagtt gaactggaag caaatggata tactcataaa
    11401 gttttatcta caattactgg agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa
    11461 gatattgcgc aagatggtgc gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt
    11521 tatacggtct tttataaagg acagtttgct ctagattcag atgaaacatc aacgattgaa
    11581 ggaagtaaag taagttttac tcatccaact tggaagggtt catttgtaga tgtaccagga
    11641 cttggatata tgtattcagt tgatgaagat gatgaaggcg tggataaagc aatgatcgga
    11701 aattggttta caaaagtagc aattccaatt gaagaagcag aactttcagg aggtacagaa
    11761 taatgtcaaa atatcaaaca acgattaaat taacgaaaaa agatgaagaa ggtaaatatg
    11821 agcaagtaca atttaaatct gctgaatttc taccaggccc agtcgtagaa gaagcagcag
    11881 aagtaatgga gggaatgcaa aatgctgtag atggaaaatc agtatcagaa gcattaagtc
    11941 gagcttattc atttattgca gatattttgt ttgaaggaca gtttactggt gaagattatc
    12001 gtcaagggat cgatgctcga gaaattgctt cgttgacagg taagttatta aaatctgtta
    12061 ctgcaggctt tgatgaaact tatacagaaa cgaaaaaaaa gtaagcgaag ctctcaaata
    12121 tccttttttt aaacattcga ttacttatcg agaattagat ttaaagacgc aattactcga
    12181 agcaggttgg acgttaccag aaattgaacg tactgacttt gacgagttaa tgcgtctttt
    12241 tgcttttcga gatgcagtaa aagagcatga ggatgttgag tactacgata acttcaccca
    12301 attttaggag gtgatacttt gaataacgat gacctaattc tgaagatgat actagatgaa
    12361 tctggtttta ctgccggtat gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca
    12421 atcgaaagaa aaagtagaaa tagtggcagt tcattaggta gtatttggaa aatatttgct
    12481 ggaagttttc tagctagcgg agtaactaga atcgtaggag ctggctttga tctaatcaag
    12541 ggttccataa gtggagcggt tgatcgagta gatacgatga ataacgctct acgaaatttc
    12601 caaaatatgg gatttagtaa ttcagaaatt atgaagaata tcggaaagaa tgggctttta
    12661 tctcaaggta ttcaaggact tcctactgct ttgaatgatg cgataagtca tgttcaactt
    12721 cttgcttctt ctacaggtga tttaactcgt tcaactcaaa tatttaaagc tttaaatgat
    12781 ggaattcttg gtttcggtgg ttcaactgat caagttaatg aagctgttat tcaattatct
    12841 caaagtttct caaacggaaa agtagacgca caaacttgga actcaatgat caacgctcaa
    12901 cttggaccta ctctttctgc tattgctaaa aagatgggaa ttacaatggg agatctgaaa
    12961 gaaggtttat ctcaaggtaa gatttctgtt gaagaattcc aaaatcaatt aatagaaatg
    13021 gataccaaag gtggcggagg acttaaatca ttaagtcaaa tcgctaaaga ttctactaag
    13081 ggaattaaaa cctctataca aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta
    13141 atagaaggat taaataaagc attagtagat tcagatttag gtggatttaa gggaattatt
    13201 gataaagtgg gcagttcaat ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca
    13261 gcagtatcat ttttaagtaa tttatttaac gaaattcaaa aatttggttc tgctttgaaa
    13321 ttcatgatgc cctttcttgt tcctgcagct accgcttttg gagcgttcat gtttcaactt
    13381 aaaggtatac cagcaataat aaaaagcttc aataatttta agaatgccat aattggcgtt
    13441 ggaaattcat taaagattat gggggcaata gctgctgcta attcatttgt tttaattatt
    13501 ggtgcagtcg ttggtgcaat agctgttttc ggttacttta tggcaaccaa tgaagagctt
    13561 agaaacaagg ttatatctat ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa
    13621 aatataaagg actggggaat tgatacttgg aattctgcta aataaatggc atcaaatgca
    13681 gttgagggtg tcaaagatgc ttggtcagga acaaaggaat ggttttctaa cacctggaaa
    13741 ggtattaagg atggggccac aggtttattt gataaaacag tagagacttc aagaaatgca
    13801 gtcgatagtg taaaaaatgc atggtctaat atgaagaaat ggttttctga tacttggcaa
    13861 agtataaaag attcagccag agaaaaatgg aatggaatca aaggttctat tatgtcagtt
    13921 gctggtccat tgattacagg tataaagaat gcatttttac atgttacttt ttatctagag
    13981 accttatgga ataatttagt tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt
    14041 atacttgcgc cggttttgtt tattacctcc atgatttctg gtggatggga agaaacaaaa
    14101 aataatatga ttgctgtttg gaataatatt aaagaaagtg ccataaatat ttgggaatct
    14161 ataaagaata tatttgtaag ttattttaca aatatttatt ttgctgcact caatatttgg
    14221 acagggttca agcttacatt gataaatatt tggaatgaag tggtaaatca agctaaatcg
    14281 atttggttaa acataaagta tttttttatt aatctttgga ttgatataaa atattttgca
    14341 attcagaaat ggattgaatt gaagttttct attattcaaa cttggatcga tttaaaatat
    14401 aatgccatta ctacttggaa taatattaaa cagtttttca aagatacttg gaaaaatatt
    14461 aaagatacgg catacaatac gtggatttct ataaaaaata ccatgattaa tacatggaat
    14521 aacattaagg actctttctg gaatattgtt actggaattg ttaattctgc tgaaaatgca
    14581 tggacaaatc taaaaaatgg tgtttcaaat gctattaatc gggtaaaaga aatctttaat
    14641 tcgttaaggg aagtcaattt atttgaaatt ggcaagaaca tcattgatgg acttataaat
    14701 ggtgtaaaag aaaagtggaa tgctttgaaa gatactatca aaggtattgc aggtaatatc
    14761 aaaaattgga ttaaaggagc tttagatatt cattctccat caagatggat gcgtgatatg
    14821 gttggtaaaa atattgttca aggtattatc gttggtattg ataaagagca aagtaagtta
    14881 gatcaaacca tgacaaattt agtaaaaaca ccgtctgttc aacctgtagt aaatggaata
    14941 agtcacctac caattacaca aacaaagcaa aatatatctg ctagtgaaac aaaagaaatt
    15001 catttacatt taaatgttta tggagattta cctgattcaa tggttcgaaa gattactaca
    15061 aaaattaaaa cagaattgac aagacaaatg aaacgagatg ctggtgcagt aggaggaaca
    15121 ttatatgcaa cttaagagag gacaattttt tattaatcaa cattattctt ctgaatttaa
    15181 tgtgtatatt caaaacaggc ctgcttctgt ttcagctagt cgagtaattg aattgagaga
    15241 gcgagaagga aatgattcta tcattattga taaaggctat tacaaaaatg tgactagaaa
    15301 gattgagtgt tactataaag caccttcaat tgatatggtg caggagtggg aagatcgaat
    15361 cactgaatgg ttagatatgt cgtcttatag tgattttatt ctttactatg atgaacaata
    15421 tatttatcaa gctgtagtaa tagaaggacc tgaatttaaa ggaacaagaa aaacaggaaa
    15481 tattgtccct tttgaattta cagtgagtat tcgaccattt aaggaaaatt acaatggtag
    15541 atttactatt cgacaaacag aaacttttga aatttataac ccagagaagt attcttcaaa
    15601 accgcttatt aaattgagtg gttctggaga tgcttctttt tatattaata aggataaata
    15661 tgatttgaaa tcgttggaca gagaattatt tatagattct aaattagaag aggcttatcg
    15721 aaaattagat ggtaatttag aacatcagga tcaggtcacc ttatttttag attttccatt
    15781 tttatatcct ggaaaaaatg aaattaaatg gacgaaaaat attcattcat ttgaaataat
    15841 gcctaggtgg tggagaaaag tatgaaacca agaatatata gtcctactga aacagatttc
    15901 tcaacgaatg gcttaggaat tttaaaggat tgtacaagat gtgaaatata tgaagtagct
    15961 aatggaaaat atgaattaga gttggattat cctttaggaa ctagatttga tgaatatttt
    16021 gaaaatgact atcaaataaa agcaaagcca aacgatcaag aagagtatca tatctttttt
    16081 attgatgata aagatataga tactttttta aatacagtaa ctatttatgc tcagagtcgt
    16141 acaaatcgac ttggaagacg ggtagtcact cttgtggaag tggactctaa aactggtcaa
    16201 gaagcgatat caattattga aactaaaatg gataaaaaat ctgacatacg actttattct
    16261 gatattacgg ctgtttctag cacaatcttt gaagcgagaa atgttttaaa ttgtattgct
    16321 ggtgaacaag gatcattgct tcaatattgg ggcggagaaa ttaaacgtga accatttaag
    16381 ctttctttgt taaagcgaag aggtcgcaat aatgttggaa cgattcggta tggaaaagat
    16441 atgtctggtt tgaaggtcaa attagattgg acaggtataa aaacaagaat tattccatat
    16501 gctgatcctc aaagtgaagt aggtacgact aatcgaattt atggttcgcc agtagatagt
    16561 gcctatatta ataactatcc tgatgtctat acagaacatg ttcagtttac agaagaacaa
    16621 ggagtaaaag acattaagag cttaaataaa atagctaaaa attactttaa aacgatcaat
    16681 cctggctgtg ataagcctaa agtttctatt acggttgaat ttgataagtt gactgatagt
    16741 gaagaagcga aagaatttgc gaagattaga aattatggtc tgtttgatac atttaaaata
    16801 tatcataaaa aatataatat ttatcttgaa tcaaaggtta gtggactaca atacgattct
    16861 ttatctgaaa aaactttgaa attagaagca ggagacattc aagttgcttt ttatcagcaa
    16921 caagctgtaa ctattcagga taaattaaaa gattatgcaa cgaataatta tatgagtgat
    16981 tttaatgatt atgtttcttc aatgattaca ggtcaaggca gtgcaggagg atatgtagtt
    17041 ttatggccaa aagaaaaacc ttccaacatc tttattatgg ataatccaga tttagaaaag
    17101 gcaagagaag tactaagaat gaataaaaat gggattgctt tttctaagaa aggatggcgg
    17161 gggcctttta attcagcttg gacattagat agtatattta atgccaattt tatacaaact
    17221 ggattaatca aagcagatat ctttcaaaat tcttttaaca agactggaga tgtattgaaa
    17281 ttagttaatg gactactcca aatttggaac aacaaaaaga aaataatgga gttaactaaa
    17341 aaaggaatgg aattttggaa ttctaagggt tcaattggaa cgattggaac aactgattct
    17401 gttggtaatc cttttcctgg ggcttctact ccaaccccta ttgaagataa ttctttagtt
    17461 attcgtacaa atggagacgg caaatatatt ttgatttctc ctaaagtagg aaaaggatta
    17521 gttttattag gaaatggtaa agcaatttat tttggagact tagatgtaca aggaaaactt
    17581 acagttaaag gaaaagaaat aattcctgga caaggtggtg gtccatcggg aggtggagaa
    17641 tctactggtg ggtatcctag tgaattaaaa acagatgcag aaaagagagc ttggagaat
//
LOCUS       DANKHJ010000060        17599 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000060 DANKHJ010000000
VERSION     DANKHJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..494)
                     /gene="polC"
                     /locus_tag="PW113_002108"
     CDS             complement(<1..494)
                     /gene="polC"
                     /locus_tag="PW113_002108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2526399.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEV"
     gene            complement(706..2415)
                     /locus_tag="PW113_002109"
     CDS             complement(706..2415)
                     /locus_tag="PW113_002109"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2526400.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(2483..3751)
                     /gene="rseP"
                     /locus_tag="PW113_002110"
     CDS             complement(2483..3751)
                     /gene="rseP"
                     /locus_tag="PW113_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2526401.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(3912..4712)
                     /locus_tag="PW113_002111"
     CDS             complement(3912..4712)
                     /locus_tag="PW113_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2526402.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(4709..5521)
                     /locus_tag="PW113_002112"
     CDS             complement(4709..5521)
                     /locus_tag="PW113_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2526403.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(5716..6273)
                     /gene="frr"
                     /locus_tag="PW113_002113"
     CDS             complement(5716..6273)
                     /gene="frr"
                     /locus_tag="PW113_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2526404.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(6276..6998)
                     /locus_tag="PW113_002114"
     CDS             complement(6276..6998)
                     /locus_tag="PW113_002114"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2526405.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(7134..8015)
                     /locus_tag="PW113_002115"
     CDS             complement(7134..8015)
                     /locus_tag="PW113_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2526406.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(8114..8896)
                     /gene="rpsB"
                     /locus_tag="PW113_002116"
     CDS             complement(8114..8896)
                     /gene="rpsB"
                     /locus_tag="PW113_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2526407.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            9255..9734
                     /locus_tag="PW113_002117"
     CDS             9255..9734
                     /locus_tag="PW113_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2526408.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            9951..11042
                     /gene="phnW"
                     /locus_tag="PW113_002118"
                     /pseudo
     CDS             9951..11042
                     /gene="phnW"
                     /locus_tag="PW113_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            11035..11820
                     /locus_tag="PW113_002119"
     CDS             11035..11820
                     /locus_tag="PW113_002119"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2526409.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(12166..13857)
                     /locus_tag="PW113_002120"
     CDS             complement(12166..13857)
                     /locus_tag="PW113_002120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2526410.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(14279..15226)
                     /gene="arcC"
                     /locus_tag="PW113_002121"
     CDS             complement(14279..15226)
                     /gene="arcC"
                     /locus_tag="PW113_002121"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2526411.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(15341..16360)
                     /gene="argF"
                     /locus_tag="PW113_002122"
     CDS             complement(15341..16360)
                     /gene="argF"
                     /locus_tag="PW113_002122"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2526412.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(16451..>17599)
                     /gene="arcA"
                     /locus_tag="PW113_002123"
     CDS             complement(16451..>17599)
                     /gene="arcA"
                     /locus_tag="PW113_002123"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2526413.1"
                     /translation="NLTPDIMDRLLFDDIPYLPIAQEEHDNFAKTLQNEGVETLYLEK
                     LAAEAIDAGNVKEQFLNKMLDESHIASNAVREGLHEFLLSMETQEMVDKIMAGVRTKD
                     IEVRSSSLYDLSADDDYPFYMDPMPNLYFTRDPSASMGNGMTVNKMTFEARRRESMFT
                     EYILKHHPRFANKGIEVWLDRENPDHIEGGDELILSDKVVAVGISQRTNAKALEKLAR
                     HLFAKNSGFEKVLAIKIPNNRAMMHLDTVFTMVDHDKFTIHPAIQSKDGKMDVFTIEP
                     DGDDIKITHSDNLHETMKAALGLDDLVLIPTGNGDEIVAPREQWNDGSNTLAIAPGVV
                     VTYNRNYVSNELLRSYGIKVLEINSSELSRGRGGPRCMSQPLVREDLK"
BASE COUNT         4889 a         3729 c         3100 g         5881 t
ORIGIN      
        1 acttctggtt cgatacgaaa ttttgggaat ccgtaactga cgaacaactc ttctactaat
       61 ggcaagtatt gttgcttcaa ataagaaata gctcctgtac tgtcgattgg caagatgact
      121 ttacgatctt ttatcacagg tacctgcgtt ttcaatactt gctgtgctag tggtgtatcg
      181 cattgctgat tttccaaagc ttgggcccag taatcttgca agagctgttc atcgaatgtc
      241 tggtcatctg ctaagatatt cagtttgact tgtgcaatat ctttaaaagc gatagtcaga
      301 tgctgagtca atgaccgata tagcataata ggcaaaattt tggaaaaatg tagcgtaaat
      361 tcccataaac gcgatttacg atggaccact acactatcca tttcaccagc agtaagcagt
      421 ggatgatttt tttctgcttc ctctagttgg atttgttcca acagtttttc aaataaatct
      481 cgtttttctg acaaagatat acgccctttc tgatgagaaa caatgcaatt gatcgcatct
      541 tatcctcttc tttttcagta ccttttagta tactcgtttt ttctgagttt tacattaaaa
      601 aattggaact gcgattcaaa tcgcagttcc aattttaaaa gcgaatcatc agattgtcaa
      661 aaacagctga ttcagctatt tttattttct gtgttttaac gcttattatt cgccttggtt
      721 caagaggatt ggtaaagtat tcgccaattc ttctttacgt acttcaacca tttcgccagt
      781 tttcttgatt ttcacttcta cgattccatc cactgctttt ttaccgacag tgattctgat
      841 tggacagcca atcaaatcag aatccgcgaa tttcactcct gcgcgttcat ttctgtcatc
      901 aaccagtact tcatagccag cttttgtcat cgcttcttct agttcattcg tcaggcttgt
      961 ttggtattcg tcttttagat tcatttggac gatatgcaga tcaaatggcg caattccttt
     1021 tggccaattg attccttgtt catctgaatg ttgttctacg attgctgata ataaacgact
     1081 tacaccgata ccataacagc ccataatcac aaatttttct cgtccatttt cgtctagtac
     1141 agttgctccc atagattcac tgtaacgagt gccgattttg aatatatgtc cgatttcgat
     1201 tcctcgagta aaggcaagaa caccgttgtt gtctggtgaa ggatctcctt cttgtacaaa
     1261 acgaagatct tcataagaaa gcacagtgaa atcgcgtttt ggattcacat ttttatagtg
     1321 gaatcctgtt tcgttcgctc cagtaatggc attcgctaaa tcttggacat aacggtctgc
     1381 atatactttt acgtcatctg aaacaccgac tggtccgaca gaaccaaact cagcacctag
     1441 atatttttgt gcttcttctt ctgttgcttc ttctaaaaag tcagcagata aaaagttttt
     1501 cagttttacg tcattgactt catggtctcc acgtaccaaa acaaggacag gttgttcatc
     1561 cgcaatgaac aatacagact tgatgatttt ttgaggttca acagaaaaga aatctgctac
     1621 ttcgtcaatt gattttacgt taggtgtagc tactttctcc aattcaagct gtgtttcatg
     1681 cgattttttt gatacatagt agcttgtagc catctccaag tttgcagcat aatcactttc
     1741 agtcgaataa cagatcgtat cctccccgat ttctgagatc gccatgaatt cctttgagtc
     1801 tttcccgccc attgcgccgc cgtctccgat gatcgagcga aattctaagc cacaacgctt
     1861 gaatacttcg gtataggctt tttcgtaatc tttatatgtc tcgtccaaac tttcttccgt
     1921 tgcatggaaa gaatatgcat ctttcatgat aaactctctt ccgcgaagca aaccgaagcg
     1981 aggtcgtttt tcgtcacgat atttaggttg gatttgataa agatttaaag gtaatttctt
     2041 atatgaattg atttcattac gcatcaaatc agtaaaagtt tcttcgtgag ttggtccaag
     2101 gatcattttt cgatcattgc ggtctttgaa acgatacaag tttggtccat atgtttcgta
     2161 acgcccggat tcttcccaaa gttctgctgg caagatggca ggcatcagca tttctaccgc
     2221 tccgattttt tcaaattctt cccgcataat gcgtttcagt ttttccaata cacgatttgc
     2281 taatggcaga tatgaataaa tacccgctga tacttggcgg atatatccag ctcttagcaa
     2341 gatttgatgg cttaatactt ctgcatcatt tggaacttcg cgtaatgttg gaatcaacat
     2401 ttttgattgt ctcattctat atactccttt atttcctcaa aatagttatc gtttgttatt
     2461 ataccctatt tgtcaacttt ttctagaaaa agaatcgttg aatatcgttc catgtaacta
     2521 gcaccatcaa caacattaaa aatccaaagc cagctaatgt caatattcct tctttttctt
     2581 gacttaatgg tttcccgcga atcccttcaa aaatattcaa gactaatttc ccgccatcta
     2641 aggcaggaat cggaagcaga ttgacgattc cgagattcat tgaaagaagc gccatcaaac
     2701 taataacagt tgttacacct tgatttgctg cttctgaaga caattggtac atcatgacag
     2761 gtccgcctaa tttatctaaa ctgaaacctg taaatagtga tcctaaggct ttgaaaattt
     2821 ccaatgaacc gctgaaagct tgacgcgtcc ctccaatgat cttatccata aaacctgtat
     2881 tcattggggc ttttatacca agttggccaa ctttttcgcc atttgactct acagatttcg
     2941 gtgtcacgtc gacagattgc acctgacctt ctcgttcgat cttaaagtct aatgttttac
     3001 ctggattttt cgtaatgact gttgtcagat cattccatga atggatttct tttccgtcca
     3061 cactgactac ctcatcattt tcttttaagc ctgcttctgc cgcagctcca ttaggcatga
     3121 tctctcctac gcgattagtg ttcgtcactt gaacaccacc ttgcataaat gccagaatga
     3181 tgaataacac gattgccaaa ataaagttat tcatgggtcc agcaaagttg gtcagcatac
     3241 gctgccacaa tttagccgat tggaactgta cgtcttttgg cgcaattctg atttctgttc
     3301 cgtcgttttc aataatcgtc gcatcgtgat caacagggta ggttactact tctgtttcgt
     3361 ctccattcac ataaccagtg atagtcagct catcttctaa atcataacgg ctcaattcca
     3421 tcgggatagc atttgtcaat tggattttct tacttaaatt tatcttctct acgatgccgt
     3481 cagaatttag taaaagagaa agaggcattc ctggtgccat ttctgtttca tcatccccat
     3541 tgcctgccat tcgaacataa ccgccaatag gtaataggcg aagcgtatat gttgtcccgt
     3601 cttttgcttg atgcccataa attttcggac ccataccgat agcaaattcg cggacgagga
     3661 ttcctgatcg tttcgcaaag aagaaatgac caaactcatg aacaatcact aatataccaa
     3721 aaacgatgat aaatgtcaga atcgttttca tagctttttt ctcctttact tgttttttgt
     3781 gttttttata aaacaacgat aaaacactat aattttacca tagtatacca aagacaccaa
     3841 tagtatcaat ataatgaaaa ataaaaacct gcgtctttta tgaaattcac aaagaaacgc
     3901 aggtatactt actaaataat gccaaataaa tgcatgatag gaaatacaaa cagcaaactg
     3961 tcgaaacggt ctaagattcc accatggcct ggtaagatat ttcccgagtc tttcacatca
     4021 taatgacgtt tgattgcaga ttcgaccaaa tcaccaaatt gtccgactgc tgaaaaaata
     4081 atcgtcatca gaagcatgac aaacatacca tgattgaata gttcttttgc tgggtatagc
     4141 aataaataaa gaaatgcgac tacaacagca ctaccgatac caccgagcgc accttcaatc
     4201 gttttgttgg gcgaaatatc aggacttaac ttatgctgac cataacgtcg accaaccata
     4261 tatgctccga tatcagttgc ccaaacaata aatagaccaa atatcaaaac agccaagctt
     4321 tctgttcgtg cattgacaaa attctggaaa cctattccca catacaagct gacaagcaca
     4381 ggaaaacctg cttcatcaat cgtatacgta tttttagaaa taactgatgt tcctaaaaga
     4441 atcataacta taagataaaa taacataaat gtacttacat tctctggaag aaagaaaaac
     4501 catctttctt ttggcaaaac aagtaacact gcaccaacgg cggataaaac tccttcaaaa
     4561 cttaagagtg tcaatccttt catccggaac agctcgtaaa ctcctactac cgctaagatt
     4621 gctgctgtaa gctcaaccgc tatgcctccg atccatataa tcggaataaa caagactagc
     4681 gccacagctg ctgtgatcac acgttgtttc atgactgatc ccctttctca tcttctgttt
     4741 tttttacacc gccgaatcga cggtctcgat tttgataaga ggcgattgct tcttcaagat
     4801 gcgcaccatc aaaatcaggc caaagcgctt ttgtaaaata cagctcacta tatgcgatct
     4861 gccataacag aaaattacta attcgctctt ctccacttgt acggatcatc agctcaggat
     4921 cttgtaactc tttaggcaag aaacctgtca ttaaatgctt agcaatcaat tcatcatcga
     4981 tttcttctgc gtgtatttct ccttttgcta cttcttcggc aatttcttgg acagcagtca
     5041 cgatctctgc tctacttcca taattcaaag caaaattcaa taccattcca gtattgtttt
     5101 ttgtttgctc aattgcacgt cttacagcat cttgcgtatg ttctggcaat acgttttcat
     5161 aacccatgac atgaactttg acattttctt taatcaattc tggtacgaac gtatcaaaga
     5221 agtcgacagg tagttgcatg agaaaactta cttcatcttt tggtcgcttc cagttttcag
     5281 tcgaaaaggc ataaagcgtc aacactttca ctccaagcct tgatgctttt tttgtgactt
     5341 tctttactgt ttccatgcct tctttatggc cagcaactcg tggcaaacga cggttttgcg
     5401 cccaacgacc attgccgtcc atgatgatgg caatgtgctg aggaattggt ccatcggtat
     5461 taaattgaaa ttcgcttttt tcttgaatat atttgttttt ttgcggaaaa aaacgtaaca
     5521 ttctttttcc tcctatactt aaaagattcc cactatctta agtattatag caatatctaa
     5581 ctgtttttcc tatgaaatca ttgttttttt atttaaaaac tgaataattt tttctttttt
     5641 taatttttaa ataaaagaaa tataaataaa aacagaggaa gcggaatgat ccgcccctct
     5701 gtttttatga ttttattaaa cttccaataa ttctttttct ttatcagcag cgatcttatc
     5761 tacttcttta atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc
     5821 atcttcggta atatcaccat ttttttgttg ttttttatat tcatcgatcg catcacgacg
     5881 gatgttacga atcgcgattt ttgcattttc tgcttctttt ttcacatctt tagctaattc
     5941 ttttctgcgt tcttctgtca gttgcgggat aactaagcga atgacatttc catcattggt
     6001 tggactgatt ccgatatcac ttgccataat agctttttca acatcttgaa gaatgctctt
     6061 gtcaaaaggt gtgatcatta aaacacgtgc ttctggaatt tggattgatg ccatttggtt
     6121 caatggtgtt ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc tagcgttcgc
     6181 acggcctgcg cggatttgcc caagttcacg ttgaagattt tgtgctgctt ttttcatttt
     6241 ttcttttgct tctgtcataa ttgcatctgc catttttatt tcccccttac agttgttccg
     6301 atattttcgc ctaagattgc acggcgaata tttccgtgtt catttaaatt gaagacaagc
     6361 agtggaatat cattatccat gcttagtgaa cttgcagttg aatccatgac ttgtaatcct
     6421 ttggcaataa cttccagatg agtcagttct tcaaatttta ctgcatttgc atctactttc
     6481 ggatcagcag aataaacacc gtctacattg tttttagcca tcaggataac atctgcgccg
     6541 atttctgccg ctcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg
     6601 cctgcaaaga taacgatccg tcctttttct aaatgacgtt ctgctttacg gcgaatatat
     6661 ggctctgcga tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt
     6721 tccaacgtat cctgtaaagc taaggcattc ataactgtag ctaacatacc catgtaatca
     6781 gcttgagcac gttccatgcc catttgtgct ccgatttgtc cgcgccagat atttccgcca
     6841 cccacaacga ttgccatttc aacgcccagc tcgtgaactt ctttgatttc ttggatgatt
     6901 tctttgatta ctggtggttt gattccaaat cctgcatccc cagctaaagc ttctccactc
     6961 aattttaaga ctacacgctg atatttcggt gtgaccatgt tcattcctcc aatattttct
     7021 attgccattt taccatactt agatgatttc gacactctaa gactggtttt tcttgttttg
     7081 cttaaaaaaa gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt
     7141 ttcacttggc tcataacttc ttctacaaag ttatcttcgc gtttttcgat accttcgcca
     7201 acttcgaaac gtacgaatgt tttaacagtt gcgccttttg atgcaacgta tttttcaaca
     7261 gtcatatcag gatctttaac gaatggttgg tccaccaaag cgatttctgc tttgaatttc
     7321 ttcaagcgtc cttctaccat tttttcaacg atattcgctg gtttgccttc gttcaatgct
     7381 tgttctgtaa gaacagtttt ttcatgttct aattcagctt ctggaatttg tgattcgttt
     7441 acataacgag gattgatagc tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca
     7501 gttgttccat ctagaacagt caatacggca atacgtccgc ccatatgcaa gtatccacca
     7561 aatgcagcat tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat tttttctccg
     7621 ataacttgag ttgcttcgat caagtcatat tcaattgtac ctttatctgt tttgattttc
     7681 attgcagctt ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat ttcttttact
     7741 aaatcttgga acatttcgtt tttagaaaca aagtctgttt ctgagtttac ttcaacgatt
     7801 gctgcagtgt tacctttcac agcaactgaa gccaatcctt cagctgcaat gcggtcattt
     7861 ttcttcgcag cctttgccat tcctttttca cgtaaaagat caactgcttt ttccatgtcg
     7921 ccttctactt caactaatgc ttttttcgca tccatcattc ctacgccagt catttcgcgt
     7981 agttctttta ccattttagc tgtaacgtct gccatgttta actcctcctg tgtgtttttc
     8041 tttttaaaaa aagctgcttc aaaggagagt gaggcttgtc gcccagcctt tgaaacagct
     8101 ccatcattca aaattattct gctgaagaat tgtcgccttc aacaacatct acgatttctt
     8161 cgattgatgt tgctgtgttt tcttctgtga aatcttcttc aactacttga tcttcacctt
     8221 ggtttccttc gatgaaagca tcagccattt ttgaagtaat caatttaacg gcacgaattg
     8281 catcgtcgtt tgaagggatt actacgtcga tttcatctgg atcgcagttt gtatcaacca
     8341 tagctacgat tgggatattt aatttgtgtg cttcttgaac agcaatacgt tctttacgtg
     8401 ggtcaacgat gtacattaca tctgggattc ttggcatatc agcgataccg cctaagaatt
     8461 tttcaagacg ttcacgttct ttgttcaaac cagcaacttc ttttttaggt agaacttcaa
     8521 aagttccatc ttcttccatt gcattgattt gtttcaaacg gctgatacgt ttttggatag
     8581 tatcccagtt tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac
     8641 gagttgcttc ttctttgatt gcttcttgtg cttgtttttt tgtacctacg aataatgcta
     8701 cgccgccttc ttcagcaacg ctcttcatgt aatcataagc tgcatctact aatttaactg
     8761 ttttttgcaa gtcaatgatg tagattccgt ttctttctgt gaagatatat ttcttcattt
     8821 ttggattcca gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt aattgtttca
     8881 tagaaattac tgccatgttt gtttcctccg atttggtttt atttttcctc ttcttatctt
     8941 ctaccttgcc ggcaaacttt ttcaagcacc taccgacaat ccaataagaa tgtggatttg
     9001 ctgatgcata aagcaccctc agctattata cagacaaaag atccgtatcg caagagcaaa
     9061 tatacttttc tgctcaacat tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa
     9121 cagtagattt ctctttgttt ctttattatc ctttttccat aaaataattt taggtataaa
     9181 agacgaatat tattgggcaa tccttctttt ttttgttatg atacatgtga aatctttcgt
     9241 aaaggagaaa gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa gtcattgaag
     9301 aattcccgat ccgtacgcaa gaagaactat taaacaaact tgggcattat ggcgttaccg
     9361 ccacgcaggc aacgatttct cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg
     9421 aaaatggcgt ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag actaggactg
     9481 aggaagagga tcggctggtt caaatgattg aagacattgt attgaaagtc gaacgagtcc
     9541 actttttgac gatcgtcaat accttgccag acaatgctca tttatttgca tctgttttag
     9601 atgagatcaa acctccctct attgtcagta caattgctgg attcgatacg atcatggtca
     9661 tctcggaaaa tgaggaggac gcaaaacgag tagaagaatt tcttcaccat ccaactgaag
     9721 tatcactttt ttaatcagag cacgtattcc taatatggaa tgcgtgtttt tttattttca
     9781 atattttcct tattaaataa acctttacac tctttacaaa aatacatcat tttttattca
     9841 attttttcaa caccttgatt gaatcacaat aatcatcatt tatattggat gttaacaaga
     9901 gcagcttctt gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat atgtataaat
     9961 tattaactcc tggaccattg acgacttctg aaacagtgaa aaatgctatg ctgatcgatc
    10021 attgtacttg ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa aaattattgg
    10081 atcttgctca agtatcggaa ggaatataca caacggtctt aatgcaaggt agcggctcct
    10141 ttggggtaga atcggtatta agttctgtga tcaggccaga agatactgta ctgatttgtt
    10201 caaacggcgc atatggacaa cgaatgatca acatgtgcga atgtctgaaa ctgaactata
    10261 ccgtttatga ggtatcgtaa catcaaatcc tccaagctca tgtgattaca cagctgattg
    10321 aagagaatcc ctctatctcc gcactagcaa tggtccatag cgagactact tccggtattt
    10381 tgaataattt ggaagagatt gctgctgtca cacaagaaaa agatcttttg tttatagtgg
    10441 acgcgatgtc aagctttggt gggataccga ttcgtgtagc cgaactaggg attgattttt
    10501 tgatttccag ctcaaacaaa tgtattcaag gtgttccagg cttttctttt gtacttgcta
    10561 ataaagcgga actagaaaag cgaaaaaatg tgagtcgaag tttgtcactt gatctatatg
    10621 accagtaccg cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc cctacccata
    10681 cggttttggc ttttcaacaa gcgctgaagg aactagaggc agaaggagga atcgaaactc
    10741 gatattcacg ttatctcgcc aataatcatg tactacgaca aaaaatgaca gagatggggt
    10801 tccagccttt cattcaagaa tatcaggggc cattcatcac ctcttttctt taccctaagg
    10861 actggcgatt tgattttaat catttttatc agtatttaaa gcaaaacgga tttgtgatct
    10921 atcccgggaa aatcagcgat catgaatgtt ttcgtatcgg aaatattggc gagatctatc
    10981 cggaagatat tttggaactg acagcaatta tcaaagacta taaggagaac gttaatgatt
    11041 aaaactgtaa tttttgattg ggcaggaaca actgtggact ttggctgtat ggctcctgtc
    11101 cacgctttta gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa agaaatccgc
    11161 gaaccgatgg gaaaattaaa atgggatcat atccaacagc ttctttccct cccttctgtc
    11221 aaaaaacaat ggatagctat ttatggacaa ctacctcagg aaaaagaggt ggaggaattg
    11281 tatcaacaat ttgaacatta tctgtttaaa gatttggtca agcattctac cttgaaaccc
    11341 gacacattgg aaacgatcga acggttgaga aacgctggga tcaatattgg ttccacgact
    11401 ggatttactg ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga agggtattca
    11461 ccagacttca tggcaacttc tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg
    11521 atccataaaa acatgcagta cttcaaaaac aagtcggttg atgaagtaat caaagtcgga
    11581 gatacggtct cagatatcca ggaagggaaa aatgccggag tgttgacagt aggtgtaatt
    11641 gaaggtagtt cgctaatggg gatttcttat gatgagtacc aaaaattaga gatggggaaa
    11701 aaagatcgtt taaaacagtc tgttgctctt agattccaac aagctggcgc tgatatttgt
    11761 gtaaacaatt taaaagagtt aacagaatac attttgtacg ctgacaagaa tccagaatga
    11821 cttttataca aaatcagagg tcgtgaaaaa gctgtactgc atcaagtaat aagaacagcc
    11881 aaacaaaaat agctcctcat atttttcgtt tggttaggct tattaccgca gatgcaagct
    11941 tttgaacacc gtttattcga aaaaaaaagg gagctgtgac acgacttttg tcacagtccc
    12001 cttatttaca aacaaacagt gtttaagaga caacctctcc actaaacggt gttcattcag
    12061 ctgactctcg gtatttgctc aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt
    12121 tctctaatac tcggatcaga acgctgattt cacattctct ttttcttaca tcttatctgg
    12181 tgcatgaagt cctagcaaac gaaggtcttc tttcaataat acagtcactg cataaactag
    12241 agctaagcgt gcttcttttt gttcatcatc agctaaaatc ttcgtatgag catagtattt
    12301 gttgaatgct tgtgctaatt tgattgcatg tttcgcaatc actgacggtt cgtatttttc
    12361 cccagcagac aagaccgttt cagggtattt ttgaactaat ttaaccactt cccagctggt
    12421 atcgtcattc aatgcatagt ctgctgtttc tgaagggaca aatcctgctt tttccaacag
    12481 gctgactgca cgagcatgtg tatattgcac ataaggtcca gtttctcctt caaatcgaac
    12541 aacttcttcc aggttaaagt caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat
    12601 gactgcccca accccaactt gtttagctac agcatctttg ttttccaaat cagggttttt
    12661 ctcgctgatt tgttctttag cagattggat tgcttcattt aatacttctt ctaataagac
    12721 gatctttcct ttacgagttg ataatttttt accaccttgc gtgatcaaac caaatggaat
    12781 atggcgcata tcgtcagacc agtcaaagcc catttccttc aagacagctt tcaactgttt
    12841 aaaatgatag ctttgttcat tccctactac atatagagat tgtttgaagt cgtaagtacg
    12901 tttacggtac aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt
    12961 cttaatcaac gctggattca ggtcataagc agataggtca acgatctctg cacctttatc
    13021 ctcgttcagc aaatgttttt cttctaaaag tttgacgatt tcatccatct tgtcattgta
    13081 gaaagcttca ccgttcaacg aatcgaaacg tacttccagc agatcataga ttttattgaa
    13141 ttctttcatc gattcatcac ggaaccattg ccataattga atagcctctt catcgccttc
    13201 ttccagacgt ttgaaccaag cacgagcttc ttcatttaat tctggttctg tttcagctac
    13261 ttcatggaat tggacatata aacgaagcaa ttcattgatt ggttctgctt taacagcttc
    13321 ttctgtaccc cattttttgt aagcgacgat cagttttcca aactgtgttc cccagtcacc
    13381 taaatggttg atgcggattg gctgataacc gattttttcc ataatgaaac cgatcgaatt
    13441 accgataact gtcgaacgca aatgtcccat tgagattggt ttggcaatat taggagaaga
    13501 catgtcgatt ggtactgttc cttgattccc gatattgcta tccccataat gttctttttc
    13561 tttcactact gtttgcaaaa cttttttgct gataagttct ttattcatga aaaagttcaa
    13621 gtaaggtccg acaacttcga ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc
    13681 tgcagcgatt tgctgtggcg cttttcggta aacttttgct aatgaaaagg ctggaaatgc
    13741 aacatctccg tgttcagctg attttggatt ttctagtaat tgttcgactt gttccagtgt
    13801 taaatcatct ttgacagcgt catagacagc ttttgcgaca agatctttat tattcatctt
    13861 tattcctcct aaagtgctcc cttgattgtt aaacaaaaaa aatctctagc gacggcatac
    13921 ttagtatgcc atcactagag acgagatttc ccgcggtacc actccaattg tcctaaaaaa
    13981 ggaccagctc catctgatat cggtagacga ccgtctgttt aaaacagaat tctccaagtg
    14041 cgcttcgttg tatcttcttc attcggcttc caccacttcc gaattcgcta gccaagaatg
    14101 atacaagtac tctcttgttc aacaaatctt agtcttatta aactttcttg aattatatca
    14161 gaaaaaaaaa cgaaaggcta gtcgtttctg ataaaaaaac aatccagatg gcctaaagga
    14221 gaagaaatac cacctggata gctataaatc atttcgtaaa tacgaaagac ttaacaaatt
    14281 aatcagctac gataatcgtt ccttcttcag aagctaaaag attctcgata ttttctaaag
    14341 aagtgatgat tgccttagcg tttggttttg cttcaacaaa tgcaatagct gcctctactt
    14401 tcggaagcat gcttcctgga gcaaattgct tttcatcaat gtattgtttc atttctgaaa
    14461 cagtaacagt ttctaatttc ttttgatctg gtttttggta attgacatac acattgtcta
    14521 ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc ggctaatttt tctgaagcaa
    14581 aatctttatc gattacggct tctcgtcctt ctaatccagt agctgtttct acaacaggaa
    14641 tcccaccgcc accaacagag actgtgataa ctccttgatc gaccagcgtt tcgatgacac
    14701 gtgcttcttt aatcgagatg ggtttagggg aagcgactac ttttctccat ccacgtccag
    14761 catcttcttt gaatgtcaca gtggagtcag cattcatttg ttctttggct tcttcttccg
    14821 tgtagaatgg accgactggt ttacttggat ttttgaatga aggatcattt tcatccacaa
    14881 taacttgagt gactaaagaa accacatctt tatcgatccc tttttccttc agtacttcgc
    14941 ccatggcatt ttgtaaccag taaccgatag atccttctgt cattgcaaca catgtatcta
    15001 aaggcattgc cggtgttttc tcagaatcag cagcttgctg ctgaatcaat aggttaccga
    15061 cttgcggacc atttccgtgg gagatgatca attcatctcc ttgttcgata aatttcacta
    15121 aatactttgc tgtttccatt aacgcttctt gttgggcttt tgcacttgca tccgtagaca
    15181 agattgcatt accccctaaa gcgacaacta cttttcgttt ggccatttca ttcgtccttt
    15241 cttcagttct accgaatttt ctagataaca aaacgagcca gcgttttgtg attttccctc
    15301 aatgctgact cgttttttcg tgagatgaat gtggttatgc ttaaacgcga gggataaaca
    15361 agttacctaa tgttgctgcc atgatcgctt tgattgagtg catacgattt tctgcttggt
    15421 caaattgacg agcatatttg ctgcggaaga cttcatctgt tacttccatt tcagtgatgc
    15481 caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc
    15541 agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc taccatttcc atattgattt
    15601 ggtaaggttt caacaatttg atacgttctt cgaatttatc ttcttcaccc attgataccc
    15661 atacatcaga ataaaggacg tctgctccgt ctacaccttt atcaacatcg tcggtaatca
    15721 tcaattgtgc accggatttt tcagcaaagc cttctgccat tttgacgatt tcttcttcag
    15781 gttgtaattc ttttggtgca acgatacgca tattcgctcc taaaattgca cctgtgacta
    15841 ataaagagtt agccatattg tttcgaccat ctccgcagta agcaacagtg ataccttcta
    15901 ctgttccaaa gttttcttgg atagttaaaa agtcagcgat catttgtgtt ggatgccatt
    15961 catcagtcaa gccattccaa actggaacac cagagaattc tgctagttcc tctaccattt
    16021 tttgactgaa tccacggaat tcgattccat caaacatacg acctagtact ttagctgtat
    16081 cttctgtgga ttctttttta cctaattgga tatcatttgc acctagatat tcaggatgtg
    16141 cacctagatc gatcgctgca gtagtaaatg cagaacgagt acgtgtagat gttttttcaa
    16201 ataaaagcgc aatatttttt ccttctagat agtgatgtgg gataccacgt tttttcaagt
    16261 ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc ttcttttgta aagtcttttt
    16321 ctgctaataa gcttcttcct tggaatactg attctttcat attaaacatt ctcctttaga
    16381 ttgtttttat ttaagattaa agaggaaatc ccgacggcag ctgacagtaa gaccgccgtc
    16441 aggaaacaaa ttacttcaag tcttcacgga caagtggttg gctcatgcag cgtggaccac
    16501 cacggcctcg tgacaattca cttgaattga tttctaacac tttgatgcca tagctacgaa
    16561 gcaattcgtt cgatacataa tttcggttat aagtgactac aacacctgga gcgattgcca
    16621 atgtgtttga tccatcattc cattgttctc gaggtgcaac gatctcatct ccattaccag
    16681 ttgggatcag tacaagatca tctaatccaa gagcagcttt catcgtttcg tgtaagttat
    16741 ctgaatgagt aatcttgata tcatcaccat ctggttcgat tgtaaagaca tccatcttac
    16801 cgtctttact ttgaattgct ggatggatag tgaatttatc gtgatctacc attgtaaata
    16861 cggtatccaa atgcatcatt gcacggttgt ttggaatttt gattgctaac actttttcaa
    16921 atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc taaagctttc gcattagttc
    16981 gttgagaaat cccaacagct actactttat cgcttaagat caattcgtct ccgccttcga
    17041 tatgatctgg attttcacga tccaaccata cttcgattcc tttatttgca aatcttggat
    17101 gatgtttcaa aatatattct gtaaacatcg attcacgacg acgtgcttca aaagtcatct
    17161 tattgactgt catcccattt cccattgatg cggaagggtc gcgtgtaaag tacaagtttg
    17221 gcattgggtc catgtaaaat ggatagtcat cgtctgctga cagatcgtat agactgctag
    17281 aacgtacttc aatatctttt gtacgaaccc cagccatgat tttatctacc atttcttgcg
    17341 tttccattga tagaaggaac tcatgaagac cctctcttac agcattcgat gcaatatgag
    17401 attcatccaa cattttattc aagaattgtt ctttgacatt gcctgcgtca atcgcttctg
    17461 ccgctaattt ttccaagtaa agagtctcga cgccttcatt ttgcaatgtc tttgcaaagt
    17521 tgtcatgttc ttcttgagca atcggaagat atggaatatc atcgaacaac agacgatcca
    17581 taatatctgg tgtcaaatt
//
LOCUS       DANKHJ010000061        17006 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000061 DANKHJ010000000
VERSION     DANKHJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..125)
                     /locus_tag="PW113_002124"
     CDS             complement(<1..125)
                     /locus_tag="PW113_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2526414.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="PW113_002125"
     CDS             complement(229..540)
                     /locus_tag="PW113_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2526415.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="PW113_002126"
     CDS             complement(542..739)
                     /locus_tag="PW113_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2526416.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="PW113_002127"
     CDS             complement(1133..2860)
                     /locus_tag="PW113_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2526417.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="PW113_002128"
     CDS             complement(2853..4046)
                     /locus_tag="PW113_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2526418.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="PW113_002129"
     CDS             complement(4233..4877)
                     /locus_tag="PW113_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526419.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="PW113_002130"
     CDS             complement(4971..5105)
                     /locus_tag="PW113_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2526420.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="PW113_002131"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="PW113_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2526421.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="PW113_002132"
     CDS             complement(7236..7709)
                     /locus_tag="PW113_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2526422.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="PW113_002133"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="PW113_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2526423.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="PW113_002134"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="PW113_002134"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2526424.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="PW113_002135"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="PW113_002135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2526425.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="PW113_002136"
     CDS             complement(11756..12895)
                     /locus_tag="PW113_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2526426.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="PW113_002137"
     CDS             complement(13204..13917)
                     /locus_tag="PW113_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2526427.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="PW113_002138"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="PW113_002138"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2526428.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="PW113_002139"
     CDS             15106..16638
                     /locus_tag="PW113_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2526429.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="PW113_002140"
     CDS             complement(16829..>17006)
                     /locus_tag="PW113_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2526430.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DANKHJ010000062        16714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000062 DANKHJ010000000
VERSION     DANKHJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(251..1462)
                     /locus_tag="PW113_002141"
     CDS             complement(251..1462)
                     /locus_tag="PW113_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2526431.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="PW113_002142"
     CDS             complement(1581..2216)
                     /locus_tag="PW113_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2526432.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="PW113_002143"
     CDS             complement(2352..3005)
                     /locus_tag="PW113_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526433.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="PW113_002144"
     CDS             complement(3108..3314)
                     /locus_tag="PW113_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDL2526434.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="PW113_002145"
     CDS             complement(3390..4136)
                     /locus_tag="PW113_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526435.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="PW113_002146"
     CDS             4296..4520
                     /locus_tag="PW113_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HDL2526436.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="PW113_002147"
     CDS             4544..5305
                     /locus_tag="PW113_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL2526437.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="PW113_002148"
     CDS             5317..5574
                     /locus_tag="PW113_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526438.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="PW113_002149"
     CDS             5666..5917
                     /locus_tag="PW113_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526439.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5917..6066
                     /locus_tag="PW113_002150"
     CDS             5917..6066
                     /locus_tag="PW113_002150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526440.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6180..6587)
                     /locus_tag="PW113_002151"
     CDS             complement(6180..6587)
                     /locus_tag="PW113_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526441.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="PW113_002152"
     CDS             6748..6972
                     /locus_tag="PW113_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526442.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="PW113_002153"
     CDS             6982..7176
                     /locus_tag="PW113_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526443.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="PW113_002154"
     CDS             7179..8201
                     /locus_tag="PW113_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2526444.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="PW113_002155"
     CDS             8164..8979
                     /locus_tag="PW113_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HDL2526445.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="PW113_002156"
     CDS             8997..9779
                     /locus_tag="PW113_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526446.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="PW113_002157"
     CDS             9779..10627
                     /locus_tag="PW113_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2526447.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="PW113_002158"
     CDS             10624..10866
                     /locus_tag="PW113_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526448.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="PW113_002159"
     CDS             11009..11377
                     /locus_tag="PW113_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2526449.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="PW113_002160"
     CDS             11392..11553
                     /locus_tag="PW113_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2526450.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="PW113_002161"
     CDS             11550..11876
                     /locus_tag="PW113_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526451.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="PW113_002162"
     CDS             11873..12688
                     /locus_tag="PW113_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDL2526452.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12827..13138
                     /locus_tag="PW113_002163"
     CDS             12827..13138
                     /locus_tag="PW113_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2526453.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13162..13380
                     /locus_tag="PW113_002164"
     CDS             13162..13380
                     /locus_tag="PW113_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526454.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="PW113_002165"
     CDS             13377..13793
                     /locus_tag="PW113_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL2526455.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="PW113_002166"
     CDS             13790..14128
                     /locus_tag="PW113_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526456.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="PW113_002167"
     CDS             14125..14355
                     /locus_tag="PW113_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526457.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="PW113_002168"
     CDS             14362..14565
                     /locus_tag="PW113_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2526458.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="PW113_002169"
     CDS             14562..14858
                     /locus_tag="PW113_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526459.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="PW113_002170"
     CDS             14935..15348
                     /locus_tag="PW113_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2526460.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="PW113_002171"
     CDS             15361..15747
                     /locus_tag="PW113_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526461.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="PW113_002172"
     CDS             16298..16525
                     /locus_tag="PW113_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526462.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="PW113_002173"
     CDS             16522..>16714
                     /locus_tag="PW113_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526463.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DANKHJ010000063        16603 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000063 DANKHJ010000000
VERSION     DANKHJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(224..766)
                     /locus_tag="PW113_002174"
     CDS             complement(224..766)
                     /locus_tag="PW113_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2526464.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(771..998)
                     /locus_tag="PW113_002175"
     CDS             complement(771..998)
                     /locus_tag="PW113_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2526465.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1077..2957)
                     /locus_tag="PW113_002176"
     CDS             complement(1077..2957)
                     /locus_tag="PW113_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2526466.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3146..3760)
                     /locus_tag="PW113_002177"
     CDS             complement(3146..3760)
                     /locus_tag="PW113_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526467.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            3902..4462
                     /locus_tag="PW113_002178"
     CDS             3902..4462
                     /locus_tag="PW113_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2526468.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(4767..5081)
                     /locus_tag="PW113_002179"
                     /pseudo
     CDS             complement(4767..5081)
                     /locus_tag="PW113_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(5335..6970)
                     /locus_tag="PW113_002180"
                     /pseudo
     CDS             complement(5335..6970)
                     /locus_tag="PW113_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7409..8278)
                     /locus_tag="PW113_002181"
     CDS             complement(7409..8278)
                     /locus_tag="PW113_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2526469.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(8298..9419)
                     /locus_tag="PW113_002182"
     CDS             complement(8298..9419)
                     /locus_tag="PW113_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2526470.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(9421..9816)
                     /locus_tag="PW113_002183"
     CDS             complement(9421..9816)
                     /locus_tag="PW113_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2526471.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(9835..10641)
                     /locus_tag="PW113_002184"
     CDS             complement(9835..10641)
                     /locus_tag="PW113_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2526472.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(10634..11422)
                     /locus_tag="PW113_002185"
     CDS             complement(10634..11422)
                     /locus_tag="PW113_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526473.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(11436..11912)
                     /locus_tag="PW113_002186"
     CDS             complement(11436..11912)
                     /locus_tag="PW113_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526474.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(11984..14641)
                     /locus_tag="PW113_002187"
     CDS             complement(11984..14641)
                     /locus_tag="PW113_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2526475.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(14660..15064)
                     /locus_tag="PW113_002188"
     CDS             complement(14660..15064)
                     /locus_tag="PW113_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL2526476.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(15296..15553)
                     /locus_tag="PW113_002189"
     CDS             complement(15296..15553)
                     /locus_tag="PW113_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL2526477.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(15678..16010)
                     /locus_tag="PW113_002190"
     CDS             complement(15678..16010)
                     /locus_tag="PW113_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526478.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         5192 a         3308 c         2444 g         5659 t
ORIGIN      
        1 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
       61 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
      121 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
      181 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
      241 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
      301 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
      361 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
      421 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
      481 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
      541 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
      601 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
      661 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
      721 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
      781 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
      841 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
      901 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
      961 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
     1021 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
     1081 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
     1141 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
     1201 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
     1261 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
     1321 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
     1381 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
     1441 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
     1501 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
     1561 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
     1621 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
     1681 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
     1741 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
     1801 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
     1861 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
     1921 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
     1981 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
     2041 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
     2101 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
     2161 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
     2221 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
     2281 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
     2341 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
     2401 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
     2461 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
     2521 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
     2581 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
     2641 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
     2701 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
     2761 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
     2821 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
     2881 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
     2941 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
     3001 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
     3061 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
     3121 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
     3181 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
     3241 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
     3301 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
     3361 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
     3421 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
     3481 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
     3541 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
     3601 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
     3661 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
     3721 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
     3781 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
     3841 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
     3901 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
     3961 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
     4021 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
     4081 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
     4141 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
     4201 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
     4261 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
     4321 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
     4381 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
     4441 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
     4501 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
     4561 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
     4621 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
     4681 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
     4741 catccagttt attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca
     4801 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct
     4861 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
     4921 ggaattggat cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa
     4981 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
     5041 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
     5101 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
     5161 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
     5221 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
     5281 tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat
     5341 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
     5401 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
     5461 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
     5521 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
     5581 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
     5641 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
     5701 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
     5761 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
     5821 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
     5881 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
     5941 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
     6001 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
     6061 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
     6121 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
     6181 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
     6241 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
     6301 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
     6361 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
     6421 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
     6481 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
     6541 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
     6601 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
     6661 tacaaacatt caatagccaa acatataaga tataactaac gattaaatta ttacttatcc
     6721 aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc
     6781 cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga
     6841 ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat
     6901 aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct
     6961 ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa
     7021 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
     7081 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
     7141 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
     7201 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
     7261 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc tgggtatcat
     7321 gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata
     7381 agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag
     7441 ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga
     7501 tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt
     7561 tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca
     7621 gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt
     7681 ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca
     7741 acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc
     7801 tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt
     7861 gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt
     7921 ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa
     7981 ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt
     8041 ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa
     8101 atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca
     8161 aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat
     8221 catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt
     8281 cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa
     8341 aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca
     8401 taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa
     8461 ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca
     8521 aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct
     8581 cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt
     8641 tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta
     8701 gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc
     8761 caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct
     8821 ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat
     8881 ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa
     8941 aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga
     9001 aatggcatct caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa
     9061 ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca
     9121 gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc
     9181 ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc
     9241 atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc
     9301 ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct
     9361 ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt
     9421 ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg
     9481 tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca aagcgattga
     9541 cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa
     9601 aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt
     9661 atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa
     9721 gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc
     9781 tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc
     9841 taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt
     9901 catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat
     9961 taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt
    10021 gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag
    10081 ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat
    10141 cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat
    10201 gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc
    10261 aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat
    10321 tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt
    10381 ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta
    10441 atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct
    10501 taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt
    10561 acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata
    10621 tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag
    10681 cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta
    10741 tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta
    10801 aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag
    10861 gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa
    10921 cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata
    10981 aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat
    11041 tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta
    11101 gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct
    11161 ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac
    11221 caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt
    11281 ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga
    11341 tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa
    11401 tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc
    11461 tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt
    11521 ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg taatgcatta
    11581 tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa
    11641 tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc
    11701 tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt
    11761 ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc
    11821 atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa
    11881 cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt
    11941 cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat
    12001 aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga
    12061 cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga
    12121 aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt
    12181 gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga
    12241 tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg gtcaattgct
    12301 ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact ttcagttcat
    12361 tatcattaaa gtgtctattc aattgattgg agaattttct tatattcatt cctaaaatca
    12421 tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt ccaacaataa
    12481 aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact aaagagtaat
    12541 agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca agtatccttt
    12601 ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata tccttcttaa
    12661 tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt tcaagaatct
    12721 gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt cttgttgaaa
    12781 cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga taaattttct
    12841 ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac tcttgtaagt
    12901 atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata tcaatggcgt
    12961 cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt gccgttagat
    13021 ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta cacattaact
    13081 taaataaaat aaccaaaaca tttttttgaa tttttgttaa ataaatatcc cctatttgaa
    13141 taaaacgatt gactaattga acttctcgac tacaccaact attaatatca ttgatgaagg
    13201 aatataattt atcgtccgat gttaattgga tttgataaat ccaagaattc aaaaagatag
    13261 acgttatttg aagataataa tagtcaaatt ctatttgaaa atctctctct atttccttaa
    13321 catactgtct aaatcgaatc tcttgatagg tgggatatgt taaagctgaa ttattttgga
    13381 tatcaacatt cttttgtcta aaatatctat catatttctc aaaaagctgc tgcttatcag
    13441 tgtctttctt ccacgtttga taaagatact ctataagttc gttgatatta aaaacttcgt
    13501 ctactttttc caaatcatcc atttgaatat attttaattc attctgtctt ttcataacct
    13561 tcatgccgtt gaaattcacc cattgtggta aatgatacat ctgaatataa agttcttcca
    13621 aatcatctaa gaaataaaag gcattggcac aggtcaattg aatgatattt ttcaaattgc
    13681 caacattttc actaaagcga tagtttaata aaaaatctaa taattgttga gatatcagta
    13741 ttttctttgc tagtctttct tcttctgatt taaaaaatat cttgattaac tgttttcttt
    13801 cataaagaga acgactgtct aatgttggaa gtgttagctt tacaggtatt cttctcaaca
    13861 aagttgataa tagtttatcc tcaggtggtt ctgatgttgc aaatataatc cagcaacttg
    13921 atgtgtacca gttttcatta tcacccatcc gatgaaattt cctcgtatcc ataaactgaa
    13981 aaagtttctc ctgacattca ttgctcaatg catgaatttc atctaaaaat aagactccat
    14041 tattagctaa ttctgctaaa ccctttcttc catcttcggc tcctgtatat gcacctttta
    14101 catgcccaaa aaaattggac aagaaaagtt ctggattatt ggcatattca ctacaattaa
    14161 ctgaaatgaa agatgccttc tcttctatat aattattggc caacaaatcc ttatacacat
    14221 aatttgctaa atagcttttt ccagttccag tctcgccatg aagtaaaatg ggtaagccat
    14281 ttggcggata gtagattgct gctttaattt gagcaattac atttcgcaga cttccttgac
    14341 ttccgataac cctttctatt ccaaccctaa aagagctatc ataccatttt tccgcttcct
    14401 ctgaagagct aaaaatttgt tgatttggaa ctttctccgg taaagaaaag aagattactg
    14461 gacgagtatt gatttttcca aattctccat cttttacgaa ttcattcaaa tattgagaga
    14521 caatattacg actaacattt aatttttcag atattttctg tgctgtattg ttcccccatt
    14581 tatttttaat ctcagagttc tctatgacat aattgacaat gcgttctttt aattcttcca
    14641 tcacagcacc tcctcgacac tattcgaatt ttgccgacag tatttttcca taaatatgac
    14701 ttgctaaaaa ggctttgttt agatcattca attggaacgt tgcttgtttt gcttgatccg
    14761 ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc caaaatatca acgattgttg
    14821 caatcagatc ttcttttgat tcttcatctg aaagtaattc acgatacaac atcgtttgtt
    14881 tgcttactgt gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca
    14941 aacggtcgga taaatattta tagaaaatca gccccaataa ataattttta tattctgacg
    15001 catccatttt actacggaga ttatctgccg cactaaataa cttctgattt aattctgcac
    15061 ccaaaaagat acactcctat ctcttattta aagttgctat ataccctctt attttagcat
    15121 actcagtttt agaaaaagca aaaaaataaa gaaacttatt cttgataaca agctacgtga
    15181 cgtatatccc taacacaaaa atagccatat cctttggttt ggatacggct atttgatatt
    15241 actcattcaa ttatcaaatc acagtaggtg tgaatctcat cgggatcggc aatatctact
    15301 tctgattagg atcaaaggtg attttctcaa taaaggtgtg atacaaagct ttaacttgct
    15361 tcttctcact ctggataaga aagcaatcca aactagtcaa tatctgttgg acatctttga
    15421 cttgaatcgg ttccccttgt tgttgaaaga tccctaagat ttcattctct cgaatttgta
    15481 actgtcttcg tttttcagtc aattcattta aacgatcttt taacatcgga aacaattcgg
    15541 gactatcttc caaagcagct tcctgttttt gctctaatag ttagattttg cgtaccattt
    15601 tcttctttta ttttccttcg attgctgatt ttaatatagt ctaaaggaaa tttatattta
    15661 ttgtattctt tataacttca agagaacttc gtttgagtat tactgtattt ttttaattcc
    15721 ataagaaaat tagttattac ctgccaccta atcagcaaat ctgaacctga gtcaatataa
    15781 ttaatattat tttccatctc caacattgta agtactgttg gtgttaatat agggactttg
    15841 tacaattgta tttttttgat tttatcatct gcacaaagga gattttttaa atctaatcct
    15901 tcttttattt caaaagttga attttttaaa tctataagag taaaaccaat tccttcatac
    15961 tgaatttctg aaaatcgaat ttttctccca gttgatagat caaggggcag aaaacctttt
    16021 attacattgt gagtgtcacc atcatttaca atgtcattag gaattttgat tttagaattc
    16081 tgtggagcac ctattggaag ataataattc attataattt tgtagtcttc atgaggaaat
    16141 aaagtacttt taggattgct aagatattct cgtaccgaat ctaagaatgg cttatccatt
    16201 gaatcttcat ccccatttaa actaaaaaac attcctacaa ttgcttttgc aactctaagt
    16261 gaattcattt tctttattga atgtgttaaa tcagacttac cattctttaa acttgtattg
    16321 ttataaagga aatttttatt gtcttctgca aatctaatat attcctcaac atactcacta
    16381 ccaaaaaaac tattacattt actacataac gtattggaat ggcaacagtt tttttgacaa
    16441 attttataag gtgcagaact tctttccgta tgctattccg attgtccttg acaatgagcc
    16501 tcctcggatg tgacgatctt cggcaggagc taacagttaa agttagactg cctcgctagc
    16561 gttagcacat ccgagctcat tatcaaggat tcgctgcgcc aat
//
LOCUS       DANKHJ010000064        16378 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000064 DANKHJ010000000
VERSION     DANKHJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..722
                     /locus_tag="PW113_002191"
     CDS             <1..722
                     /locus_tag="PW113_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2526479.1"
                     /translation="GSYANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIP
                     LTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTT
                     SDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMD
                     YNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEG
                     NTKGGAQDFHDKRWYLNIPQR"
     gene            979..1626
                     /locus_tag="PW113_002192"
     CDS             979..1626
                     /locus_tag="PW113_002192"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2526480.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(1951..2097)
                     /locus_tag="PW113_002193"
                     /pseudo
     CDS             complement(1951..2097)
                     /locus_tag="PW113_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2336..3028
                     /locus_tag="PW113_002194"
     CDS             2336..3028
                     /locus_tag="PW113_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2526481.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(3310..3382)
                     /locus_tag="PW113_002195"
     tRNA            complement(3310..3382)
                     /locus_tag="PW113_002195"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3347..3349),aa:Lys,seq:ctt)
     gene            3763..4332
                     /locus_tag="PW113_002196"
     CDS             3763..4332
                     /locus_tag="PW113_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2526482.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            4319..5179
                     /locus_tag="PW113_002197"
     CDS             4319..5179
                     /locus_tag="PW113_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2526483.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            5369..6073
                     /locus_tag="PW113_002198"
     CDS             5369..6073
                     /locus_tag="PW113_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526484.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            6078..7913
                     /gene="walK"
                     /locus_tag="PW113_002199"
     CDS             6078..7913
                     /gene="walK"
                     /locus_tag="PW113_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2526485.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7910..9226
                     /locus_tag="PW113_002200"
     CDS             7910..9226
                     /locus_tag="PW113_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526486.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            9227..10096
                     /locus_tag="PW113_002201"
     CDS             9227..10096
                     /locus_tag="PW113_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2526487.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            10151..10960
                     /locus_tag="PW113_002202"
     CDS             10151..10960
                     /locus_tag="PW113_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2526488.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(11063..11692)
                     /locus_tag="PW113_002203"
     CDS             complement(11063..11692)
                     /locus_tag="PW113_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2526489.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11862..13154
                     /locus_tag="PW113_002204"
     CDS             11862..13154
                     /locus_tag="PW113_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526490.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(13230..13892)
                     /locus_tag="PW113_002205"
     CDS             complement(13230..13892)
                     /locus_tag="PW113_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2526491.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            14117..14401
                     /gene="groES"
                     /locus_tag="PW113_002206"
     CDS             14117..14401
                     /gene="groES"
                     /locus_tag="PW113_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2526492.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14452..16077
                     /gene="groL"
                     /locus_tag="PW113_002207"
     CDS             14452..16077
                     /gene="groL"
                     /locus_tag="PW113_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2526493.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5612 a         2711 c         3419 g         4636 t
ORIGIN      
        1 ctggttcgta tgctaatgca aatagtcctg tgcagattga gttacaacac acacatgata
       61 aagcaacgtt tgagaaaaac tacaaggcat acgttgaatt agctagagat tcagcaatga
      121 aatatggtat tccacttaca ctagacacac cgtataatca accagggatc aaatcgcatt
      181 tatgggtaac acaaaatatt tggggcgatc atacagatcc ttacggttat ctttctgaaa
      241 tgggcgtaag taaagaaaaa ttagcctatg atttggctca tggatttacc gatgaaaatc
      301 cgacaacttc agatgataaa ccagtcattg atccaactcg agcaggtgct gcaaatccta
      361 ccctgacaga tggaacaaat tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa
      421 acttacatgt ggctggttgg cacatcgcta actataaata cgagtatatc ttcattatgg
      481 attacaatac tgggaaagaa ttagctcgag taaaagctga tggaatatat agaccagatg
      541 taaatcaagc ttataatact ttaggaaatg ttggctatca tgtatctttt aacatgcgca
      601 acttccctaa taagaaagtc tatgtaatga tgcgggcaac gaatgatcca gaagggaaca
      661 ctaaaggcgg agcacaagat tttcatgata aacgctggta tttaaatatt ccgcaacgat
      721 aaaaatagcc cctcgttttt gaggggaagt acataatgca aatttgtcca taacttattg
      781 attatcttgt aaaaatgcgg taatttaata gttagtgaga agaggtgctt aattatagtt
      841 aaaaaagagg aaatagaagc agcatgtaac caagcaaatg ttaggtttat tggagaagct
      901 ttatctggat atcctaagta taaatactta cacaactggt tacaaagtaa aagtttttat
      961 cttaataaag aaagatgcat gcctaataaa aattataggg tttataaaag aggaactatt
     1021 gtatatgttg attttggggt gaacgtgggt tatgagttat ctggtaatca ttttgctatt
     1081 gttttaaata ataaagataa ttcgaaaaat ggtgttgtat gtgtagtacc aatctcttca
     1141 aaaaagaaaa gtaattatgt atctgtagga aaaatattag aagttgctag tattaaacaa
     1201 tttataagcc aagctgataa attaaaagag aaacttagga taattatttt actttcatta
     1261 aatagaggca tcattgatga aagcgatttg aatagtttta ttaaagatat tgaagctaaa
     1321 ggtgaaaagc tttcagtgga agaagtagaa aaacgagctg taaaacataa tttggtttgc
     1381 gagacaaggg aacaagtaat taaagaaata aaatcattat ctgaagatat ggtaaaatat
     1441 cagaaggtgt ttaattgcta caagaaatat acacaagaat cctatgtaat gccacttaat
     1501 atccaaacga ttagtaaaaa tagaattaga agaattaaca agtttgatcc ttctggaaat
     1561 atgaaggcac ctggaaatgt cctcaatgaa gtagatgaaa gtttaaaaaa atattttaca
     1621 aagtaaacga tatttttgtt gacatttttc tgttcgttag ttaccatgta gttatagaag
     1681 gacgtaagcg cctattttta atgtacacat cgggacgtat gcgcccacat agaaacatag
     1741 ttgagagaca tctatatggt gtctcttttt ttattaatat gcaaaaaagt ctagtttttt
     1801 caataatatt tatgtaataa taaattagcc accacaacaa agaatgaaat ccattattat
     1861 ctagtctagt ccattctttt tgtttgcagt atttgtgatg gctttccgta cccttagctc
     1921 agttggtcag agcagacggc tcataaccgt ccggtcgtac ttctcgtaat ccaggaaggc
     1981 cgtcagctcc gtccgcagta gcgtgtttac ggccagctcc agatgggagc ggaataactc
     2041 gttcaaatca cctttggtgg ctagagtctg caggagttct gtaataaaat cgttcatagg
     2101 gaagtcctct tttctgtgaa tgggtgtggt aactttattc tacagaaggg acttcctttt
     2161 ttctatttac acaaaatatt ttatactctc aaatgtttgt atgcagttac atctgacttt
     2221 attcgaatta aggaaaagat ttataaaaat aaaataaaaa aatatatatt taaattgact
     2281 tattgtgaaa tcattaacta taattagttc agtttatgtt aaggagaatg atgatatgaa
     2341 taagaaaatt gtagtaagtg gattaattat aggtggatta gtgttagcag ggatcgcagg
     2401 gaaaaacttt gccgatgaca ttttgttatg gggtggagaa gataatattg aacaaatcaa
     2461 tcataatttg gagtcattag ataaagcact agaaaataaa gagcaaaata ttagtagttt
     2521 aaattcacga ttatcttcaa ataatcaaga acttgagcaa gcaaaggcta acgttgaaga
     2581 atataaacaa aaagtgttga ctttggaaaa tgataaaaat caattaacta tagagaaaac
     2641 aaatttagag aatcaattat caggaaaaaa tgctgaattg caaggtaaac aaaatgaaat
     2701 caatgctaaa attgatgaaa taaatcaaaa aatagctgaa ataaatcaat taaacactga
     2761 ttggaataat cgtttacaaa cagaacaaaa taaatttaat gatgctcaaa atactgttaa
     2821 tagtttaaat caacaaatta atcaattgtc aaatgaaaaa aatactatta aaaaccaatt
     2881 gcaaagtact gaacaaaaac tagaaaaagc gcaatcagaa aacagtaatt tgaataatta
     2941 tattgaaaaa ctggaaaaag ccaaaagcga tgtagaagac acagcaaaca gatcacaaga
     3001 aatcgtgact gaacatacag gaaaatagat gcatttttta gtttaaattt ctttaacaaa
     3061 atagtatttt gttgctaaat aatttttatt tgaatattct acaatgatat ctcccctcaa
     3121 aatggagggg aagtacatac tgtgataatc tacaggttta ttgactgatt aggatgttat
     3181 taaaaaataa aatactaaaa tgcatacccg aatttcatac ccgaaaaata aaaaatgcca
     3241 ccacgtacac tcttttcgaa actacctaag tagccaagaa tgtactggtg acgtgggttt
     3301 ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga gtcagctgct
     3361 ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca ctaagaattt
     3421 taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt ataggaaatc
     3481 attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa caaagttctt
     3541 gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc gtctccccgg
     3601 cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt tttttgtttt
     3661 catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg tttatcataa
     3721 gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag
     3781 caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg ggaagaactc
     3841 cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta tctttcacct
     3901 gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa ttacgttaaa
     3961 ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt agcattttta
     4021 cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga agggatattg
     4081 ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga tgaacaagaa
     4141 gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca aaaattcgac
     4201 caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa
     4261 cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga gaaaacaaat
     4321 gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag aagaattgat
     4381 aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc aggtatatac
     4441 agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg aaatccctgg
     4501 tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca aaaaagacgg
     4561 ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat ataatgtggt
     4621 tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata cgaaaaatat
     4681 tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag aaggagtaga
     4741 atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag ttttctttaa
     4801 tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg cttctattgt
     4861 aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga tttattacac
     4921 agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt acgtcaaaga
     4981 aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg cgaaagaaga
     5041 agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc aaatctactt
     5101 tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg gtgtcggtgt
     5161 acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc
     5221 ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat tttaattttc
     5281 tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat gagaaggtat
     5341 gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa
     5401 gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg tttatactgc
     5461 atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt tgattttact
     5521 tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac gcaaaactta
     5581 tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag tcctaggctt
     5641 agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat tagttgcacg
     5701 tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag aggcagcacc
     5761 tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg tgacgaaacg
     5821 aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact tggcgaagca
     5881 ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt atgattattt
     5941 tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa
     6001 tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc
     6061 agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc aattttaaga
     6121 tcgctattac ctttattctg attttattga tctcgatcga aattattggt gcctacttta
     6181 tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat caaacggtgg
     6241 aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca gatgatgaag
     6301 aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat atcatcgaaa
     6361 tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg
     6421 ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa cgttatgttg
     6481 ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg tccccgaccg
     6541 gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa
     6601 tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt atttcaatga
     6661 tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg agagaacaag
     6721 ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga caagatgaac
     6781 ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa gctcaagata
     6841 caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca gacggcgtca
     6901 ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt tccttgctag
     6961 atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat atcgaagaag
     7021 aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac cgttcaacca
     7081 gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc cgtcgcgaat
     7141 caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag
     7201 aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca ccgttgacaa
     7261 gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac
     7321 caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg atcaatgacc
     7381 tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa tatgtcaatt
     7441 tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga aatcaggaaa
     7501 aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa gtagatacag
     7561 ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat tcaccagatg
     7621 ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta agtatcaccg
     7681 atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag
     7741 tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct atttctagag
     7801 aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga aaagggtcga
     7861 ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa tgagaatctc
     7921 tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat acttctctta
     7981 tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga caaacactca
     8041 aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac
     8101 atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt accgattgca
     8161 attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag gagacaaatc
     8221 acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata atgcgccttt
     8281 tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga acttggcaaa
     8341 agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata aaaaaatccg
     8401 atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga attggacaga
     8461 tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa cagaaccatc
     8521 gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt atagttatat
     8581 cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac cagatagcgt
     8641 gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa caatgtctat
     8701 ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa aagaaaatgc
     8761 tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt tagggaacct
     8821 cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg aaggcttccc
     8881 ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg aaacgaatgg
     8941 ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga taccttcaga
     9001 cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag ctaagggagc
     9061 agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg tcaaagaaac
     9121 gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata actggtattc
     9181 agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg atttcaaaaa
     9241 aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat tcagcagtta
     9301 tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg aatcaatcca
     9361 acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg agaacaagca
     9421 aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga attatcgcaa
     9481 ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa
     9541 atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc
     9601 cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt atgtgtacac
     9661 gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc aagagtatga
     9721 aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa aaaacgataa
     9781 tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc agctgcgcga
     9841 aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca ataacaaaat
     9901 accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc tgaaagtccg
     9961 ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt
    10021 ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa
    10081 agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta agaagggaag
    10141 taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag caagtggaag
    10201 ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact
    10261 tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg accaattaga
    10321 tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg tcttggctcg
    10381 taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg aaccactgat
    10441 tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca gaacattcgg
    10501 cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc aattttatcg
    10561 tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt gcagtgatta
    10621 tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc atgatcttga
    10681 gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag gagatagagg
    10741 acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta atcggaccaa
    10801 acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg ctcatttaac
    10861 aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg aaatctatga
    10921 tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat caataaaaat
    10981 ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt tattttttta
    11041 gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc ttttcatgtt
    11101 tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt
    11161 cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct gtacgtttgc
    11221 catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt ttagattgtt
    11281 tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt gcatttttag
    11341 gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc
    11401 caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg gacaaagaag
    11461 tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca acatcaaaag
    11521 atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga acatctgatg
    11581 gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac aatgcttcgt
    11641 ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc ataaaaaacc
    11701 tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt
    11761 ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata gttaaattaa
    11821 gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag tggttagatc
    11881 gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact gctttgcgta
    11941 atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc ttatttgctc
    12001 attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct atgtatttta
    12061 cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt gccattgcat
    12121 acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca gccttgtctg
    12181 cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg acaggtgctg
    12241 aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat gcacgtttag
    12301 taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt tggcagtctt
    12361 ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt tcagcggttt
    12421 ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc cagcttccca
    12481 ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt gttccgtttt
    12541 tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt cccgtcttgc
    12601 aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg acggtggcaa
    12661 atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt acgggtgctg
    12721 gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag aaatacagag
    12781 aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca attatttttt
    12841 ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca ccggtcgttt
    12901 caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt acagcagtcc
    12961 aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga gtgcctgcag
    13021 cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca tttttgaaaa
    13081 aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa
    13141 agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa atcaccaatc
    13201 taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac agctaattga
    13261 acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca gatccgacca
    13321 gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag cagatgacca
    13381 tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca aaaatttgaa
    13441 tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat gatgggtcca
    13501 gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat gatattttgt
    13561 gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac agcgatgctt
    13621 tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt tggagcagaa
    13681 gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata aaaaatagct
    13741 aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc agacgtagat
    13801 tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc atagcaaaaa
    13861 atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag ggataacaag
    13921 catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa taagcagctg
    13981 tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga gaatgagtat
    14041 tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa aaaaagagtt
    14101 atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa
    14161 agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag agaaacccca
    14221 aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc
    14281 tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta cagaagtaaa
    14341 atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga ttgttgagta
    14401 aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa
    14461 gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga taaattagca
    14521 gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga aaaatcttac
    14581 ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga attagaagat
    14641 cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac aaatgatatt
    14701 gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg cgaaggttta
    14761 aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga attggcaaca
    14821 aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa agaagccatt
    14881 gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat tgctgatgca
    14941 atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca
    15001 gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca atatatggta
    15061 acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat cacagacaaa
    15121 aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca acaatcacgt
    15181 ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct tgtattgaac
    15241 aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt
    15301 aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac tgacgatcta
    15361 ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa agttgttgta
    15421 gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt
    15481 gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg cgaaaaatta
    15541 caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg tgcagctact
    15601 gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc tacacgtgcc
    15661 gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa
    15721 gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct
    15781 ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc agtgattgtt
    15841 gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg agaatgggta
    15901 aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc cttgcaaaat
    15961 gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga taaaccagaa
    16021 ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat gatgtaagaa
    16081 aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa gaggctggaa
    16141 cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt tcaaattttt
    16201 gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat acgtgtttag
    16261 agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttttgtgt gtaattataa
    16321 tgaaaatagt tatttttaat agttataata ttttatctat taaaagtaaa tatccatt
//
LOCUS       DANKHJ010000065        15976 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000065 DANKHJ010000000
VERSION     DANKHJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..450)
                     /locus_tag="PW113_002208"
     CDS             complement(<1..450)
                     /locus_tag="PW113_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2526494.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="PW113_002209"
     CDS             674..1465
                     /locus_tag="PW113_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2526495.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="PW113_002210"
     CDS             1431..2384
                     /locus_tag="PW113_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDL2526496.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="PW113_002211"
     CDS             complement(2782..3756)
                     /locus_tag="PW113_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2526497.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="PW113_002212"
     CDS             3871..4305
                     /locus_tag="PW113_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2526498.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="PW113_002213"
     CDS             complement(4430..5452)
                     /locus_tag="PW113_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL2526499.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="PW113_002214"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="PW113_002214"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDL2526500.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="PW113_002215"
     CDS             complement(6546..8468)
                     /locus_tag="PW113_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL2526501.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="PW113_002216"
     CDS             complement(8555..10495)
                     /locus_tag="PW113_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDL2526502.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10783..11523)
                     /locus_tag="PW113_002217"
     CDS             complement(10783..11523)
                     /locus_tag="PW113_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDL2526503.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11536..12627)
                     /locus_tag="PW113_002218"
     CDS             complement(11536..12627)
                     /locus_tag="PW113_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDL2526504.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12617..13723)
                     /locus_tag="PW113_002219"
     CDS             complement(12617..13723)
                     /locus_tag="PW113_002219"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDL2526505.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13742..14404)
                     /locus_tag="PW113_002220"
     CDS             complement(13742..14404)
                     /locus_tag="PW113_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HDL2526506.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            complement(14415..15194)
                     /locus_tag="PW113_002221"
     CDS             complement(14415..15194)
                     /locus_tag="PW113_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HDL2526507.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15223..15573)
                     /locus_tag="PW113_002222"
     CDS             complement(15223..15573)
                     /locus_tag="PW113_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HDL2526508.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15589..15945)
                     /locus_tag="PW113_002223"
     CDS             complement(15589..15945)
                     /locus_tag="PW113_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HDL2526509.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4739 a         3346 c         2691 g         5200 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa ttattatcta cattaacaat ttccgtaatt aattataaaa agcttattcc
    10741 atttaatttc attctggaat aagcttctta ttttctaatg agttagtgaa taattttttt
    10801 taccatgctt aatagtatct ttacgtctcc tatacgcgta tctcctgttt ctttatctat
    10861 aattgaacta taaacatgag gaatcacttt ttttacacct gcatcgattg caattttcag
    10921 aatttcttca aagtttgaaa gatcaattcc tcctgttggc tctaaataga attcatattt
    10981 tgcgcaagct tccgcaactg ctttaaattc ttccctatgt gctaagcctt tcatcgggaa
    11041 atacttgatt gaagtacctc ccatgtcttg cagcatttga atggcagttt caataggcac
    11101 ttcagctgct ggcgcttgtg aacttaacgg acctgtagca atattgacat agccgatctt
    11161 acctgttggt gaaaccaatc cattgatgac tgtatcattt tggcccaata aagcacgaga
    11221 agtccccacg ccagtaaata cttgattgac atgttgtggt tgtaaaaccc cagataaacg
    11281 cgaaaccatc tgactttggt ttggatctcc agcgcctaat ccaactgaca acgcattatt
    11341 ggttgcgtct tggtaccttt tcatatcttc gattgctgct tcatcgctag cataattttt
    11401 cgatagcaca cctagtacga catgcccttc cgctgcttca tagcatgctt ttgcattttc
    11461 aactgaatga gctaatacat ttaaacatac acgattttta taataatttg ggataactga
    11521 caatttaaaa gcctcctaca tgatttctct caatcttttt ataatttttt ccatttcgtt
    11581 ttggctgacg gaacgaatgt cgaactcaat gattccgtta tttgcttgat attctcgggt
    11641 gtagactgct ggatttcgtg cttttaattc ctgaatgact tctttcgcac ttttatcgcc
    11701 attaatcgtt acacttgctc gataaatatc tctgccggca ccatcttgaa ctattttcgc
    11761 tgaaaggtca ggaagttgat tgattgcgtt aacaaaaggc gctaatcgtt gcttcatgga
    11821 gtcaccggat tcgctcccat tttttaaata atcttctagt gcctgggtaa agcctaaaat
    11881 attatcttta ccgattttca ttgctcggcc aataccttta gactgtagcc gaatccattg
    11941 aatatacttc ttttttccta tgaccagtcc ggcactcgga ccttcgattg cttttgcccc
    12001 agagtaaatc actaagtcaa cgcccatttc gctatatttg aataaatctt cttccgcagc
    12061 tgcatcaacg atcaatggca aacgatgagc tttggcaact tctaccattt ctgccaccgt
    12121 cagcatactt ttctgaactg catgatgact tttaatatat agaagcgcag cagttttttc
    12181 cgtaatcatc atggaaacat gttccggtgt acacatattt gcatagccag cttctatgac
    12241 ttttcctcca ccttgagcaa ccattacttc aaccggtgtc ccataatcta cattatgtcc
    12301 cttaggtatg atgatttccc gtctagcgat tttttctgta tagggatgat agacatggta
    12361 catgtcgccc tgtccgatta aagcagcaac agattgagca atccctgctg aagcagaaga
    12421 aacgatttgc gcatcttcca ctttcagtaa ttgagaaaga taagcgccgg ttttgatggc
    12481 aagatcactc atctcaaaaa aatgctcgcc tccaaatcgt tgtgctgcta ggacactttc
    12541 agatacctta gaaaccccta ggatcgtcat ttttcccgat gcattaatga cttcttttaa
    12601 atcaaacttt ccatagctaa ttgtcatata tgacgcctcc aataattgtt ttaatcggta
    12661 aaatttgttc tttagctata cgtgtaaatc cgttggaatc tttcaattcc ttttcatttt
    12721 caacaatctc aaatatcgtc aaatctgcat cataattgac agctaactgg cccttattct
    12781 tcaaatgaaa attccgtgca ggaactgctg taactttttc aatgatttct tcccaagaat
    12841 agccgactac cctcaatttt tctaaagtag ttgctagatc aaaaactggg ccatttgtac
    12901 gattgcggat gtagatatcc gtactaatag aggtagcttt cattcctgct tttagtgcgg
    12961 tttctgccac atggaaatta aagctatccg tcccatgtcc aatatcaaag acgacaccat
    13021 tattgtaagc atcccagaca aagtttttaa tttgatctgt ctttggatct aaaataccgt
    13081 ttggttttcc attaaaacaa tgggtcaata catcgcctct ttccattctt gacaatactt
    13141 cttctaactc aggaggtgca gaaccgatat gaaccatcaa tggcagatca ttgttttctg
    13201 tctggatttt tttagctaat tctaatggct taatgccatt ttcaccaaca actgtcttac
    13261 tcattctcgc tttaatcccg acaataaatt ccggcagttc tgctaacgca gtacttacta
    13321 gatcttcttg aatctttgtt agatcagcaa gttcatcttg tttgacaatg ccccatttag
    13381 agatattcat tagcgcatac acatttgtct tgaccgattt cgtcaattga taaaattcac
    13441 ggatgttttc tgctcccgta gttccagcat caatgaccgt cgtcacacct ttttcgaccc
    13501 ctatctgatc aggatagtca taataaagat tcatcttttc atagcagtgg acatgatcat
    13561 cgatccaccc agcggataaa taatactttc cctccaagtc aactacttct tgcgccggct
    13621 gatttatttc tgctgcaact gctttaatca tgcctttttc tatagcgacc tctattttag
    13681 atccatcaac taaccttcca tttttaatca aagtatcaaa catttaatga ccctctttct
    13741 attaatttac tgccgggaag attgctccta aaatagcacc tccagcaatt ggttttttcc
    13801 aagcatagaa tcctagtgct cctaatgtag cgccaatgcc gattggaata gatgctgcac
    13861 acgcgcttaa tataattaat ggacccaaaa aacgtccaga tgaatttcct gcccccatca
    13921 tgacgtcagc accaaaagtg gaatttgatt gattgatcgt aaatttacga atcaagacaa
    13981 tgatgccacc aacaatcaaa cctagaacca aaccagtcaa taaagagagt gcaaaattat
    14041 cgattggtgc cgctattttc atacttaaga gaattgctgg taccccaatt ccgatcccag
    14101 ttaggatcga accgcctaag tccaagatcc caactaaaga gccttctaaa attctggcaa
    14161 ataagaaact agcaccaaac gctgccgctg cgccatagga tccgccatcc agcccggctt
    14221 ttaacatcgc cacgatcgaa acttcattga atgcacccac accatacatc acatacatgt
    14281 gtgttcctgc aaatacagca gcggacataa ttcctacgat tagcggaaaa gaccagtctg
    14341 caaaccaaaa gctcttttgc cgctttgctt ctaatacttt tgcttgttct tctgttgtct
    14401 ccatgattct cctcctattt cacactaaag atgttgtgca agttatcaag ccaattaggc
    14461 acttgaagct ggaagctctt gatcatttcc atatcaaatc cacggaaaaa tccactgaaa
    14521 ataaataata aaacaatagc gatcatcatg ctcttcgtga cgcgattcca accgctctca
    14581 tcgacgcctt taccaatcaa gattcctaga acaacaccag gtaccgcatt tcccataatc
    14641 aattgcgcga taccaccaaa aagagtgccc caaaaaccag ttcgtttccc tgcgtctaaa
    14701 gctgccaacc aaaaaataac tggcattact gtattgatca gaatcgtcgc tgctggtacc
    14761 aaaacggaaa ccgccgtcac ttgtaacgcc tctggaattg ctgcagctgt tgtgtttaag
    14821 aaggctacca ccactgcccc gataaccgct ccaatgatcc ccattttttt cggatcatgc
    14881 atggtctggg tgacatcttt gttttttgct agaagtgctg ccgctgccca attgggaatg
    14941 attcgatgcg tcacatcttg tgtgaatgca ccagcaccaa ctgaagaagc ccatgcatta
    15001 aagaaaaagc ctaaaccaaa tgaaaaatga gaagctggat cacccatgca ggcattcatt
    15061 tcgcctaatg ttctaaaagc tcctaaacct tgtgtattag gtgcatgaaa cattcgagct
    15121 gccccgacag atgctgcaaa accaagtaat cccccgataa tgaccgattt gactagaatg
    15181 attacgaaat ccacgttatt ctttccccct tatgattcaa aattagattt ttttagttac
    15241 aaatggaatg gcaatcccat ttggatcttc agtcatttgc tggctgaacg atatgtctgc
    15301 caattttatt tctgttacag ccacagtaac agagagttcg acttcatagc taatacgttg
    15361 ccgtgggaag aagaaaaata gaaagcgttc tgtgtatttg ttttcgaccg cctttaaaat
    15421 atccacattg actggttcga ttcgcaataa tacgtttgat tgttccatta ttttgttttg
    15481 aatttctgct aacgctgatg caaaagcatg ttgctttgtt tcccctttac cggagacgat
    15541 aactttttgc tgaattgtct tttgaacgct catttcatgt ccctctttct attttccatg
    15601 ctttttgata aattcttctg tcagacgttt ccctaattcc tcttgatcca taaagccgaa
    15661 acctagaaca gttttgccat ccttgatagc tgtgatccct tcttcaatcg aacgcatacc
    15721 atgtcgttct ggatagccat attttgttgc tgctgttaat gcacctgcac caccactacc
    15781 acaaaaagag atacccatat ctgcatgctc ttgttgcatc acatcgccca aacgcatatc
    15841 tgcccccatt cctggtacga caactgcttt tccaccagct gcttctaccc ctttggccac
    15901 attttgtcct ttacccatac ggtctgcaat aactacttta atcatgattg tttcctcctc
    15961 gtaaaatatc atttgt
//
LOCUS       DANKHJ010000066        15900 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000066 DANKHJ010000000
VERSION     DANKHJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(52..522)
                     /locus_tag="PW113_002224"
     CDS             complement(52..522)
                     /locus_tag="PW113_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2526510.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="PW113_002225"
     CDS             complement(681..1139)
                     /locus_tag="PW113_002225"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2526511.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="PW113_002226"
     CDS             complement(1310..2455)
                     /locus_tag="PW113_002226"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2526512.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="PW113_002227"
     CDS             complement(2468..3385)
                     /locus_tag="PW113_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2526513.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="PW113_002228"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="PW113_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2526514.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="PW113_002229"
     CDS             4477..4647
                     /locus_tag="PW113_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526515.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="PW113_002230"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="PW113_002230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2526516.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="PW113_002231"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="PW113_002231"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2526517.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="PW113_002232"
     CDS             6911..7792
                     /locus_tag="PW113_002232"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2526518.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="PW113_002233"
     CDS             7789..8505
                     /locus_tag="PW113_002233"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2526519.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="PW113_002234"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="PW113_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2526520.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="PW113_002235"
     CDS             9222..10223
                     /locus_tag="PW113_002235"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2526521.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="PW113_002236"
     CDS             10220..11581
                     /locus_tag="PW113_002236"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2526522.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="PW113_002237"
     CDS             11541..12692
                     /locus_tag="PW113_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2526523.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="PW113_002238"
     CDS             12667..13836
                     /locus_tag="PW113_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2526524.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="PW113_002239"
     CDS             14011..15390
                     /locus_tag="PW113_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2526525.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="PW113_002240"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="PW113_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DANKHJ010000067        15658 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000067 DANKHJ010000000
VERSION     DANKHJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1421)
                     /locus_tag="PW113_002241"
     CDS             complement(<1..1421)
                     /locus_tag="PW113_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2526526.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="PW113_002242"
     CDS             1673..2854
                     /locus_tag="PW113_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2526527.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="PW113_002243"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="PW113_002243"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2526528.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="PW113_002244"
     CDS             complement(4993..7704)
                     /locus_tag="PW113_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526529.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="PW113_002245"
     CDS             complement(7714..8415)
                     /locus_tag="PW113_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526530.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="PW113_002246"
     CDS             8601..9200
                     /locus_tag="PW113_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2526531.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="PW113_002247"
     CDS             9281..10294
                     /locus_tag="PW113_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2526532.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="PW113_002248"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="PW113_002248"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2526533.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="PW113_002249"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="PW113_002249"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2526534.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="PW113_002250"
     CDS             11742..13115
                     /locus_tag="PW113_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2526535.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="PW113_002251"
     CDS             13472..15616
                     /locus_tag="PW113_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2526536.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         4611 a         3128 c         2911 g         5008 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tccctttt
//
LOCUS       DANKHJ010000068        15138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000068 DANKHJ010000000
VERSION     DANKHJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            4..2376
                     /locus_tag="PW113_002252"
     CDS             4..2376
                     /locus_tag="PW113_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526537.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="PW113_002253"
     CDS             2437..3897
                     /locus_tag="PW113_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2526538.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="PW113_002254"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="PW113_002254"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2526539.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="PW113_002255"
     CDS             6128..6277
                     /locus_tag="PW113_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526540.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="PW113_002256"
     CDS             6352..6945
                     /locus_tag="PW113_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2526541.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="PW113_002257"
     CDS             6958..7857
                     /locus_tag="PW113_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2526542.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="PW113_002258"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="PW113_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2526543.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="PW113_002259"
     CDS             8723..8983
                     /locus_tag="PW113_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526544.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="PW113_002260"
     CDS             9428..9634
                     /locus_tag="PW113_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2526545.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="PW113_002261"
     CDS             9682..9885
                     /locus_tag="PW113_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526546.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="PW113_002262"
     CDS             9901..10338
                     /locus_tag="PW113_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526547.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="PW113_002263"
     CDS             10331..11038
                     /locus_tag="PW113_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526548.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="PW113_002264"
     CDS             11418..11597
                     /locus_tag="PW113_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526549.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="PW113_002265"
     CDS             11922..12185
                     /locus_tag="PW113_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2526550.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="PW113_002266"
     CDS             12179..12529
                     /locus_tag="PW113_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2526551.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="PW113_002267"
     CDS             complement(12553..13167)
                     /locus_tag="PW113_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2526552.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="PW113_002268"
     CDS             13481..13903
                     /locus_tag="PW113_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526553.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="PW113_002269"
     CDS             13913..14116
                     /locus_tag="PW113_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526554.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="PW113_002270"
     CDS             14327..14911
                     /locus_tag="PW113_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2526555.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3060 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaaa
//
LOCUS       DANKHJ010000069        15022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000069 DANKHJ010000000
VERSION     DANKHJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..626)
                     /locus_tag="PW113_002271"
     CDS             complement(<1..626)
                     /locus_tag="PW113_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2526556.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            complement(1200..2384)
                     /locus_tag="PW113_002272"
     CDS             complement(1200..2384)
                     /locus_tag="PW113_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2526557.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2409..3416)
                     /locus_tag="PW113_002273"
     CDS             complement(2409..3416)
                     /locus_tag="PW113_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2526558.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(3588..3947)
                     /locus_tag="PW113_002274"
     CDS             complement(3588..3947)
                     /locus_tag="PW113_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526559.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3919..4290)
                     /locus_tag="PW113_002275"
     CDS             complement(3919..4290)
                     /locus_tag="PW113_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2526560.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(4307..4609)
                     /locus_tag="PW113_002276"
     CDS             complement(4307..4609)
                     /locus_tag="PW113_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526561.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(4626..5096)
                     /locus_tag="PW113_002277"
     CDS             complement(4626..5096)
                     /locus_tag="PW113_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2526562.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(5071..5349)
                     /locus_tag="PW113_002278"
     CDS             complement(5071..5349)
                     /locus_tag="PW113_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2526563.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(5346..6395)
                     /locus_tag="PW113_002279"
     CDS             complement(5346..6395)
                     /locus_tag="PW113_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2526564.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(6352..7281)
                     /gene="tadA"
                     /locus_tag="PW113_002280"
     CDS             complement(6352..7281)
                     /gene="tadA"
                     /locus_tag="PW113_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2526565.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7413..9167
                     /gene="ade"
                     /locus_tag="PW113_002281"
     CDS             7413..9167
                     /gene="ade"
                     /locus_tag="PW113_002281"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2526566.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(9265..9927)
                     /gene="trmB"
                     /locus_tag="PW113_002282"
     CDS             complement(9265..9927)
                     /gene="trmB"
                     /locus_tag="PW113_002282"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2526567.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9997..10779)
                     /locus_tag="PW113_002283"
     CDS             complement(9997..10779)
                     /locus_tag="PW113_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2526568.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10983..12194)
                     /locus_tag="PW113_002284"
     CDS             complement(10983..12194)
                     /locus_tag="PW113_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526569.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(12187..12924)
                     /locus_tag="PW113_002285"
     CDS             complement(12187..12924)
                     /locus_tag="PW113_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526570.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            13062..13496
                     /locus_tag="PW113_002286"
     CDS             13062..13496
                     /locus_tag="PW113_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2526571.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            13489..13833
                     /locus_tag="PW113_002287"
     CDS             13489..13833
                     /locus_tag="PW113_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2526572.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            14013..>15022
                     /locus_tag="PW113_002288"
     CDS             14013..>15022
                     /locus_tag="PW113_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2526573.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4757 a         3062 c         2550 g         4653 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      721 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccgttt tttcgcaaca
      781 aaaaacttga ttttcatacc tgtgctttca cgattcaggc aaagtacagg tggaaaatca
      841 agtttttatt attcgatcaa tgaatgtttc ggatcggttt tctagctaaa cataaagcga
      901 agaataaagt cgacagccca cgaggctgat ctaaaaatgt tttttctttt tacatatgta
      961 atgtccctta taaagatgaa tgaaaataaa atattgggat tttcctgaaa atcaataaat
     1021 aaaatgccag tcttttccct tttgaccact tttccaagca aaattcccta agaagatcct
     1081 gtaaaaaaga agggaaattg aagttaaatt accattttgc ggtcttctcc gcaaaaaaaa
     1141 cacatgcaag aattccctct tgcatgcgtt tcatctttta tcctaacgaa tgctttggct
     1201 tagccgcgca gacgctccac atcacgggca atcattagtt cctcatctgt tgggatcact
     1261 aatactctta cttttgattc ttttgtagag atatctactt cttcaccacg caattcattc
     1321 aacgctggat cgatttcaca accaaaccaa gacatacctt tgatgatttc actacgtaca
     1381 tgtccgtcat tctcgccgat accagcagta aagacaattg catcaacacc attcatcgtt
     1441 gtcacataac cgccgatgta ttttcggatg cggtccacga aaatgtcata agctaaacga
     1501 atatgttctt tttcatagtt cttttcaaga tcacgcatat cactagacaa tcctgttagg
     1561 cctagcagac cagatttctt gttcaaaaca ttgatcattt catcaatatc aatctctaat
     1621 ttttccatta aatatgggag aacagctgga tcgatatcac ctgaacgtgt acccattgtc
     1681 accccagcta atggtgtgaa tcccatagaa gtatccatgg atttacctcc atcgactgct
     1741 gtgatcgata caccatttcc taagtggcaa gtgatgattt ttagttcttc gatcggtttg
     1801 ttcaataatt ttgccgcacg ttccgacaca taacgatgac ttgtgccatg gaagccatat
     1861 tttcttactt tgaaatcttc ataataagac accggtaagc tatataaata attttgcttt
     1921 ggcattgttg agtggaaaga tgtatcgaat acagccacac tcaaaatgtc aggtaaaatc
     1981 tttttaaacg ctttgatccc cattaagttt gcaggattat gcaaaggagc aaattctgct
     2041 aattcagcaa tgcggtccat tactttctca tcgatcacta ctgaatcccc ataagtttct
     2101 cctccgtgaa caacacggtg ccctactccg gtgatttcat cataagagct taaaatttgc
     2161 agttcaatca actgatctaa cagcattttt actgctactt cgtggttttc gatatccatg
     2221 atttgttcga atttttgatt atcgccgtac ttgatggtaa aaatagaatc ttgtaaaccg
     2281 atacgttcga cgatcccttt cgcaactacg gtttcttctg gcatttgata taattgccat
     2341 ttcaaacttg aactcccagc attgattgca attgttttag acatatgact ctccttctcg
     2401 ttgaatgatt acagagcaga tgatttccag tttttaaact cgttgaaaaa ttccgtcacc
     2461 ttctctggtt cttttaatga agcaagtttc acaagcaaga cttcttttac ttgttgtgct
     2521 tgtggtcctt ttttctgtaa gatcaaaata cttttttgcg attgtttatt acggaacaat
     2581 tcatctggca attgaatcat tccttgaaga taaccttctt ctttaaacca ttttttgatt
     2641 tcttcacttt gatccgtttc taaaaatcca ctcggcatca aaaacaaacc aaaaccatcc
     2701 ggcttcacat atttcattga ttgctctagc aataaatgat gtgcataact atggccttct
     2761 tcggtacttg ttaaaaactc ttttgctttt tcgtcattcg gataataacc gattggcaaa
     2821 tcgctgattg caaaatcgac aggatctatt aataattcct gcaacccgtc ttgatgaaag
     2881 tattgaacat ttgcttgtgt caaatcactt gtagaagctg cgacagccaa caatgtatca
     2941 tcaatatcca caccaattcc ttctgtttgg taccccgcat tcgataggtt taaaagaact
     3001 gttaataaca agttgcccat tcctgccgca atatccagaa tcttaaccgg tgctttttta
     3061 tttgtgtata gttgttcaat aagaaaaaca aataaaaaac cgatactatc aggagtgagt
     3121 tgatggttcg cctgcaaatg ttctgtctgg ctgcccttca ataaaagtaa ttgagacagt
     3181 ctgcgccatt cttctggctc gaaagaaagt ttcttcaact cttcataaag agctgtgatc
     3241 cgttgcgttg tctcttttgt cggcactcca tccaccacac gtacttgata atcatctatt
     3301 aaattttctg cattttcgac ataggcttct aaaaaggaag ttcccaatgc attctgtaaa
     3361 agctgaatag cttccaggtt ttgattaaac ccttgttcca ttttttctgg taacatggtg
     3421 cacctcattt ctagatatat tataacaatt ttttcacctg tttaattcta tcgaacaatt
     3481 gacaaaattt caatcgtttt cactgttttt tatttgtgaa acggcaaaca tttttttatt
     3541 aaaaacagtg ttataacaga aaagagggcg ttttcacgaa cattccatca ttctgtacta
     3601 gtatctgtat cattttttac ttcttctgtg aaccgaaaga taaacggtga gacttccacc
     3661 tctaagaata gggactgttg tacccactga tatgttacag atccagttgt gtagtaaaaa
     3721 actccttccg aactttcggc aagcgtagac tgttccgata aagccatctg cttcattatt
     3781 tttgctatat aataattttt gattcgtata ttaagatcca ttgacaaacg gtagctttct
     3841 aatgctgcca tcaataaaaa cgtacataaa agaaataaaa gcaagacaga aaataaaata
     3901 gagcccttat aacattttct actgatcgtt ttttgtccat atggcatatc catcttctcc
     3961 attttcaaag tgtacataaa aagaaattgt gtgttctttt tccaataatt gaagctcagt
     4021 gatacttgtt aacataggtt gatgtccgcc ggaagttctt cttttacgta tcattgcctg
     4081 atatctttcg atgatatact gtttatcctc gatcgtgtag tgcagttgct gatttttcac
     4141 agagacattg tgactgctct tgagttcttc ttctaattgt gctaaaaata ttagccactc
     4201 tttttgatct ttacgctgca aaaattcact tacttggatg gtttgctgaa tcaaaagatt
     4261 gaatgtgagc aatagactgc tcaatatcat cagtccaatt agacattcaa tcagcgtaaa
     4321 tgctggaatt acttgttttt tcaatactga tcgcttcttt ctccatattg acaaacactt
     4381 tttcgattac tccattatta tcgacaattt ggatatcgta tgggatcatt tcttcccttc
     4441 gctctggtct tttgtgatac atccgctgcg tcttgatatc ttcataaatc attcgaataa
     4501 atagcagttc ctccccactt ttcttttctg cctgaagcaa aaacagctgg ccaaagctca
     4561 ctaagtagac acagacagaa gcgatcataa gcgctgtaag ggactccaac aaaacaaatc
     4621 ctgttttatt caattctttt tgtatagcga ccactcccta tctggaattg atacgtaatc
     4681 gtccgctctt tttcttccca ataaaattga taagctttta gtgaactttc attcccggtt
     4741 ctagcagcaa aagtaataga agcatggcgt ttcaaggtaa tctcatcagg aatatctaat
     4801 acttcccaat ttattttatc tggattcgga acatgaaaaa tgatttgatt ctcttcacta
     4861 ttccaataaa agcctgtttg gatctgattc acaatagcga ttttttgcgt agaatagatt
     4921 cgtttttcaa attggccgaa aaattgatag actgccaatt cattcttcca tgcagaaaat
     4981 gcgagaactg gaaaagaaag gatcgttgta agtaataaaa tcagtaagag tgattcaaac
     5041 aacgtatatc ctgatgctaa aaccttacct tcaaggccgc tgatagattt ctgcatgttc
     5101 tcgtttgata tagccttcat tcaacaattc ctccacagta ggaactcttc cattattttg
     5161 aagcgcatag agctcttttt gtgtgtctac caactggatg atcgcttcac gactttcttg
     5221 gtctacatga ttcctataac gcgacaaatt agggacgaac aacaacacta atacactgat
     5281 gatcagtaag acgattagca tttcgatcaa tgtaaaaccg ctataataag atatttttct
     5341 ctttttcatg gcaaaacctc ctctatgctt ccgtaaacag gtaataaaac agcggcataa
     5401 actaataaaa tcaaagccgc tacgcccaaa aaaatgattg gctgaataaa atgcatccag
     5461 tgatgaagac gtttgaaaag ctgttttctt gtccattcgc tgtaaaaaat cagttcttta
     5521 cctaaacttc cctttgcctc tccctgtaaa acaattttac tgaattcttc tgatagaaaa
     5581 cgataattct ggaactgctc agccaatgac atacctgcct ccaatccttg aatcaactta
     5641 gaggcaagat gctgaatcag actttcttgt ttcgtttcct ttaagcagac tatgatttgc
     5701 cttaattcga aaccttcatg aaaaagcttt ccaatctcta gtgacaaata agccgattga
     5761 tacaaggaat atatcggtcc aatcaataat tgtctgctta tccattcact ctgatcaaca
     5821 ggagacaact tcatcagacg ataccttact agtaaagcca atagagtaac cgctaggatg
     5881 aatagtagaa cataccaatg aaagacttgt atcagctgaa taccccaatg ctcttgcatg
     5941 accattcctg aaccaataag ctgaggcaaa acgaacaacc gcatactaaa caagatacct
     6001 agcaaaaagg tgagcaatag catcggatat gcaagcagct ttttcaaatc attgcgaaat
     6061 ccttcaacaa gccgaaactg tttggcaatt tccaacaaag ttgtcggcag attcccatga
     6121 cgttcagcta actcgatctg taccagttgc tctgctccat atcctatttg cgaaagtacc
     6181 tcactaaacg cttgcccttc ctttagctga tgttggatat ttgccaaaat aacaggtgga
     6241 aacgaacgga tcgtgagtaa taaataaagg gattcttgca gactaaaacc agataaaagc
     6301 agttctccca tggtttgtgc aaactggttt tgctggcttc ttgttaatcc tttagaatag
     6361 ttctgactgc caaatatttt cctgagcttc ttcataactt tcctcccatg atttttttac
     6421 tgattcttcc tccataaatt gataactcca taaaacagaa ggatggtgat tggcatcttc
     6481 caaaagttct tgataaatga caccagctaa acattcagat agctcttctt ttttctttgt
     6541 cagttcatgg attctgacca atgtaccttc aagattgcgt gcatgtacgg ttgcaaaaac
     6601 ccggtgccct gttagtgctg ctcttaccgc agcatgtgca gttgattcat ctcttatctc
     6661 gccaatgatt agcaagtctg gacgatgccg taatgcgagt ctcaaaagtg tatcataagt
     6721 ctgtgctatt ttcggattga tctgtagctg cagaaaaaac ggttcttcta tctctacagg
     6781 atcttcgata gtaatcactt gcaatttttc ttccaaagcc aaccgataca ttagagaagt
     6841 cttgcctgat ccgactggtc cgctgaaaag gtataatcca cgtctgacgg ttttctgtaa
     6901 aatagttgca aaatctcgag aactaaagca acgatggggt gtttcaaaag taccgtataa
     6961 tagccgaatc actaaactct ctcgttgaag gtaatctcct acagaagaca accgcagacg
     7021 aacttcctgt ttttctaaag aataagtaaa tgcacctaac tgagcttttc ttttttctcc
     7081 gatatccatc tgtcccaaat acttaaatct tgagatcagt tgctgtccat ctgccgaaga
     7141 gactttcttc cagaatgttc gtttttttcc atagcgaaaa tagatgtaaa atacttctga
     7201 agctggcaat atatatacgt cttgggcatg tttggcaaaa ctttcactta tcatttgatc
     7261 cgataatcgt tttatttcca ttattctccc ctccacaaaa aacatacgtc tttttgatct
     7321 ttttgtttca aataaaatga atttacaaaa aaatattggc aaaaaccgaa tttatgttat
     7381 acttataaaa atttatcttg ggaggcactc cattggaaac aacattaagg aaatatatcg
     7441 aagtagcaag taaaaaaaag cctgctgacc tagtgatcaa gcatgctaag atcgtcaacg
     7501 tctttacgaa agaaatcatg gaaaaagatg tagcaatctg cgaaggaatg atcgtaggaa
     7561 tcggtgaata cgaaggaaaa agcgtacata atgctaatgg gcaatatcta gtgccaggat
     7621 tcatcgatgg ccatgtgcat atcgaaagct cacttttatc accaagcgaa ttttcaaaag
     7681 tatccttgct tcatggagtt actactgtag tgacagatcc ccatgaaata ggaaacattg
     7741 ctgggagtac aggtcttgag tttatgatag aagatgcaag acaaacactc atgaatatct
     7801 tttttatgct accttcttgt gtacctgtaa ccccttttga gaccaatggt gcaaatttaa
     7861 atgcaaaaaa attagcccct tttttacaaa aacccgaagt tttaggctta gctgaagtca
     7921 tgaactatca agcagtagca aataatgaaa cagacatcct agaaaagatc caattgatgc
     7981 accagcataa gaaaaaaatt gacgggcatg cagctggtat cggcatggaa gagttaaatg
     8041 tttatcctgc agcaggtatt cggacggatc atgaagctac aactgcaaaa gaagctaaag
     8101 aacgtttgga tcttggtatg tatttaatgg ttcgagaagg gaccgttgca aaagatctgg
     8161 catcgctact accggcgatc acacctgaaa attcaagaag atgcttcttt gtcaccgatg
     8221 ataaactgat caatgattta gtaaaagaag gatcaatcga tcatatcgtc cgtcaagcta
     8281 ttgcacttgg aatcgatcca ttacaagcca ttcagatggc aacattgaat gcagcagaat
     8341 gttttgattt gaaaacacta ggtgcgattg cccctgggta tcaagctgat ttcttcttaa
     8401 cagacgatct taccaagctg ccaattcatg aagttttcta taagggaaca ttagttgttc
     8461 aatctgggca actccaagac catttatttg ctgatcattc aaattcatat acgtgttgtc
     8521 tgccaaaact aaacgctcaa ccattaaatc cacattcgtt agagctgctt ctttcttctt
     8581 cacaagccca tgtgatcgaa attatcccga atagtctagt gactaaagat ttggtagaaa
     8641 aagtggaccg acaaggcgaa cgattcattc cttcaatcga aaaagaccaa ttaaagattg
     8701 cggtgatcga gcgtcaccat caaacgggaa acattggatt aggcatagtg aaaggatttc
     8761 agctgaaaca aggagcgata gcgacaacga ttgcccatga ctcgcataat ctagttgtcg
     8821 ttgggacaaa cgacgaggat atgctgtatg ctgctaatct tttgattcaa aaaggtggcg
     8881 ggatgaccgt tgtcaacaat cagcaggaaa tagcttgtgt ttctcttcct ataggcggaa
     8941 tcatgacaca ggaacctttt tcgatcgtct atgaacagtt gaatgaatta ataactaaag
     9001 cacatcaact aggagcagct aaaacctttg atcctttttt gacactttct ttcttaacat
     9061 tatctgtcat acccgaattg aagatcaccg acaaaggatt attttcattt tctcgcttcc
     9121 aattgattcc accttgtgga aaaaccgatg aaataaatac attttaaatc aaataaatcc
     9181 cagaaaatac attacatctg taatgttcat tttctgggat ttatttatgg atattaatgt
     9241 ttttatgaaa gcagaaagtt agtttcacag acttttcttt ttggtcttat cttgaaattg
     9301 agcttctact cgataaatac gttgaccttt tgaagaaaat ttttcttcat actccgtcat
     9361 aatattgcct tcaaattgac tttgatgtaa atctaaccat acctgtttga ggatcatgcc
     9421 atattgagaa aaacttgcca gagaatactc aaacaatcct tgattatctg ttttgaaatg
     9481 gatttctcca tttggacgaa gaatctgttc atctacagca agaaaagtct tatatgtcaa
     9541 tcgccgtttt tcatgacgtt tttttggcca tggatcggaa aaattcagat agatttgatc
     9601 tacttcgctg tcagcgaaat actctgtcaa cgctgatcca tccacatgta gaagtttcag
     9661 gttaggaagc ggttgttcga tcaacttgtc tagtgcaatc gaaacaacac tgacctgcat
     9721 ttctactcca atataattga tttcaggatg ggcttttgcc attccagtga tgaactggcc
     9781 tttccccata ccaatctcaa tgtggattgg atgatcgttt tcaaacaatt cattccattt
     9841 tcccttccac aatgttggat cactaattac aaaattcggg tgagccgcta acatttccgc
     9901 tgctcccgga cgatttcgga cacgcattat ttatctccat tcctttcctc taattaaaaa
     9961 ggtctggcca aatctccgca gagtttgggc caaacctcag ccactgaaaa ttcgttttaa
    10021 ttgtaaaatt tcttcgttca tccgtcgatg atcttcacgt ttgcaatatc tggtgatttc
    10081 ttggagcaaa ttcatggccg cataccaatg aaatttttca agcatttcgt cagtcgggcg
    10141 tatgccataa ctgatcagcc actgattcca gctagacagt ggcacataat ttcctaaaat
    10201 cgttgcaata tccaccgcag ggtctgcaaa catgattgaa tcccaatcga ctagatataa
    10261 ataattttta catactaacc aattacgatg gtttacatct ccgtgtacta ctgataagtt
    10321 ttccttatct attgacggga tctgctcacg aagaaaacga tggacaattt gcagaaattg
    10381 atttttctgg agttcttccg gtaattcttt ttcatatttt tgcagcatct tctccggcgt
    10441 catgacacga ccaccgatac gggtcagcat ggttttaaga gaatttgaat gatgtaaatg
    10501 gtacaaaaca tcaatcacgt cgtttctttg accaatctca ttaggagtca gcaagcgacc
    10561 ttccagccac tcttgtgctg tcaaagtatc gccattcccc gttcgttttg tccaaacaag
    10621 ttttggtgta attccttccc ttgaaagagc agccaacatc ggagtcgtgt ttcgtttaat
    10681 aaagactttg tcctcatcac gcatgcctat gtatgttttt cctgtgtcgc ctttgatggg
    10741 acgtaaccgc cactcttggt ctaactgaaa ctccatcatg cattctcccg tcctcttact
    10801 ctttgttttg cagtacttat tattctagtc gcctgaacac tacacgtcaa gcacatatac
    10861 gttctttttc atttaattgc cattcaaaaa accagctttt ctcaaaaaaa atagaacgaa
    10921 cagcattctt actgtcaacg ctctattttt cattctcatt tttaccctac acagctttgt
    10981 tttcagtctg acatcttctt caaacgagtt ggtacatata gtttaacaaa gagcatagta
    11041 acaatcaaat aagccgctag cggaacccat cgctgctgcc catctaatac gactgcagca
    11101 atcaaaccaa aaataaccga cgcgataatt aatacccatg tgagtagata ctgaatcgcc
    11161 tgcttcttct gattcttatg gattggataa agctgaacga gaaccatata gcggaactgg
    11221 ttcgctaaag gtaacagttg gaaaccgatc aaatataaaa ataagaaacc gataatcaga
    11281 ctaaaataaa agtcgactac tccggcaatc aataacccac ctataattac taatcggaaa
    11341 taaagaccac taaaatcaga acctcttgcc aatcttcttg caaacaagta aagataagtg
    11401 ttttttgatt ctcttttaat atgagtcaat ataggatcca aatagcgtct tcttttgatc
    11461 tttgccgtaa tttcaggaac atctgtgaat aaattgataa attgatagat ctgatgcaac
    11521 ctatgttctt ctacatgcac cattttctcc caatccaatc ctttttcttg ttgcttccac
    11581 aaaaaccagt aatatagact tgcctgtact aatgcacctg ctattcctgc ccatattagc
    11641 caataaaatg atagaaacaa aaagatcaga gaagaaaaaa accagcctaa tttccaataa
    11701 cgaataactt gttgatctag tcgaaacaat tctgctctct tgatttgcag atgactgaat
    11761 ttcaaactcc ataatatgat cagatagacg aaaaacatcg aaaaggattg ctccgtacta
    11821 accaccacta atggcatggc caatccgcat gccaatatca aaaagatcaa cggaaaaatg
    11881 caagaatagc gaaaagctcg ttccaaatag gtttgcattt ctttttcttt aggaagtaaa
    11941 aaaacaatat ctgctgcctc tgcaaaagta gccatcttac ccacgaataa acagagcatc
    12001 caaaatacag agattatgag ccctccaatt gcaaacggtg gctgcagggt tttcaaccaa
    12061 ttagaataat ataatcccac accgccaatc aagaaaagac aaactaaaac aaaatgatcg
    12121 ttcaatacat aacgcatata tttcatcatt ttcttttgat gtcttgctaa acgtttttga
    12181 aaaaaactac tcatgtgcct gctcctttgt taaagcaata tagatttcgt ccaaagaaga
    12241 atccggcagc tgaaattctt ttctcaacat ctcaagagaa ccgtctgctc gtatctctcc
    12301 gttatgtaag accacaaaac gatcacaata tttttctgca gtagctaaaa tatgcgtaga
    12361 catcaaaata ccggctcctt cttttttcat ttctaccatc aattcaagca atgcattgat
    12421 agctagtgga tccaagccta aaaatggttc gtcgatgata tataaagaag gctcgatcaa
    12481 aaaggcgcat aagatcatca ctttctgttt cattcccttt gaaaaattag ctgggaacca
    12541 gtcaagacga ttagataacc gaaaagtctc caacaacttt tctgctcttt tcattgctat
    12601 atccataggt atatcgtagg ccatcgcagt gacttcgata tgttctctca atgtcaattc
    12661 ttcatataaa gaaggagtct ctggaatata cccaatttgt ttgcgatatt cttccgtatt
    12721 tttctttaag gatagatgat tgatttctat cgttcctttt gaagcttcca gcagaccgat
    12781 aatgtgcttg atggtcgtac ttttccctgc tccgttcagt ccaatcaacc ctactatctc
    12841 tccaggctgt acatcgaaac tgatatcttt taacacaggg agatgtccgt acccccctgt
    12901 gacatgatcg ataattaaac tcattctttt tcctccgcca tttattttta tttattatac
    12961 cacgaagaaa atgacggtat tttgtttttt tccttacaat agctttaact tatggtaaag
    13021 ttaagctgaa gtatttattc tttatagaaa ggatgtttta tatggaaaac tgtatttttt
    13081 gcaaaatcat caatcaagaa atcccaagct ataaaatata tgaagacgac aaggtctatg
    13141 cttttttaga catctctcaa gcaacgaaag gacacacatt agttgtgcct aaacgtcatg
    13201 tagcagatat ttttgaatat gatcccgaac ttgcaggaga ggttttttcg cgtgtgccta
    13261 agattgctca agcattggaa aaagcttttc cagaaatgaa aggtcttaat atcttgaata
    13321 acaaccgtga actagcctat caatctgttt ttcattcaca tatccattta gtccctcgct
    13381 acagcaaaga ggatgatttt tctattcact ttgtcaatca tcaagatagt tatggatcgg
    13441 aagaactaaa agctattcaa gaaacaatcg taaagcaggt gtcctgcgat gattaaaaac
    13501 tttttcaaag gactgttatt tggagctgct gctggcactg tcggaggtct gttattggcg
    13561 cccaggagcg ggaatgaaac aagaaagaaa ctgatcgaag aattggaaga agcaacaact
    13621 ctgacaaatg atttaaataa cagcctgaat cattttaaaa gcgcattgat cgaaacaaaa
    13681 gaaacagctg aatctgtgat tcctgctttc caagaagcaa tcaaaaaaga tatcgaagat
    13741 tttaaattcc aagccgaacc acgtatcaca caaatcaatg aacaagtaga aaaactaaaa
    13801 gctgatctgc cagagtatcc tgaacaaata taataatgat tattttctca acagatttaa
    13861 ctatataagt gagaaaagtt tgcgtatttc ctaggaatgt gatgaaattt tgaattttgt
    13921 ttgaaactac cacgacaacg tttttaaagg aaatgggata tggtatagtt aatatataaa
    13981 aataataact aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag
    14041 ctactagtgc gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata
    14101 tcgcaacgat gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg
    14161 aatcatcgaa ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat
    14221 atggcgataa agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat
    14281 taggtgatac ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag
    14341 aatatattcg taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag
    14401 atgacgattt gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca
    14461 aattatcaag tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt
    14521 tctctaaatt agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag
    14581 ttaaattcga ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat
    14641 tgaaagacgg tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag
    14701 aatactacgt tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca
    14761 aagatcaact aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta
    14821 aagtaatcgg tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa
    14881 atgtgttgag tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag
    14941 cttcaacaaa gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag
    15001 aaacaactga ttcatcaaaa ta
//
LOCUS       DANKHJ010000070        14861 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000070 DANKHJ010000000
VERSION     DANKHJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..135
                     /locus_tag="PW113_002289"
     CDS             <1..135
                     /locus_tag="PW113_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2526574.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PW113_002290"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="PW113_002290"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2526575.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMVVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="PW113_002291"
     CDS             complement(2412..2663)
                     /locus_tag="PW113_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2526576.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="PW113_002292"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="PW113_002292"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2526577.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="PW113_002293"
     CDS             3613..3831
                     /locus_tag="PW113_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526578.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="PW113_002294"
     CDS             complement(4120..4632)
                     /locus_tag="PW113_002294"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2526579.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="PW113_002295"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="PW113_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2526580.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="PW113_002296"
     CDS             complement(7085..7528)
                     /locus_tag="PW113_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2526581.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="PW113_002297"
     CDS             complement(7529..8317)
                     /locus_tag="PW113_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2526582.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="PW113_002298"
     CDS             complement(8314..9255)
                     /locus_tag="PW113_002298"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2526583.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="PW113_002299"
     CDS             complement(9432..10019)
                     /locus_tag="PW113_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2526584.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="PW113_002300"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="PW113_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2526585.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="PW113_002301"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="PW113_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2526586.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="PW113_002302"
     CDS             complement(12936..13871)
                     /locus_tag="PW113_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2526587.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14057..>14861
                     /locus_tag="PW113_002303"
     CDS             14057..>14861
                     /locus_tag="PW113_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL2526588.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
BASE COUNT         4185 a         3291 c         2618 g         4767 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acaaccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt
    13981 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    14041 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    14101 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    14161 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    14221 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    14281 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    14341 gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat tttctaaagg
    14401 aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg gccatcagga
    14461 aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata attatcctga
    14521 tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc atcataaaag
    14581 tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta aacccaacgg
    14641 ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct tccctaaaaa
    14701 cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga aagccattag
    14761 taaccaacat gatattgata tatattttgc ggaccctgga acaccttctc aacgcccatt
    14821 aaacgagaat tctaacggga ttctgcgtcg taatggactg c
//
LOCUS       DANKHJ010000071        14684 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000071 DANKHJ010000000
VERSION     DANKHJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(99..1229)
                     /locus_tag="PW113_002304"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="PW113_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PW113_002305"
     CDS             complement(1878..3080)
                     /locus_tag="PW113_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2526589.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PW113_002306"
     CDS             complement(3140..3343)
                     /locus_tag="PW113_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2526590.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PW113_002307"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PW113_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PW113_002308"
     CDS             4880..5692
                     /locus_tag="PW113_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526591.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="PW113_002309"
     CDS             complement(6716..6868)
                     /locus_tag="PW113_002309"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2526592.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="PW113_002310"
     CDS             complement(7059..7937)
                     /locus_tag="PW113_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2526593.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="PW113_002311"
     CDS             complement(7976..9094)
                     /locus_tag="PW113_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2526594.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="PW113_002312"
     CDS             complement(9091..11190)
                     /locus_tag="PW113_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2526595.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="PW113_002313"
     CDS             complement(11241..12062)
                     /locus_tag="PW113_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2526596.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="PW113_002314"
     CDS             complement(12059..12868)
                     /locus_tag="PW113_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526597.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="PW113_002315"
     CDS             complement(12889..13383)
                     /locus_tag="PW113_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526598.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="PW113_002316"
     CDS             complement(13395..13883)
                     /locus_tag="PW113_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2526599.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..>14684)
                     /locus_tag="PW113_002317"
     CDS             complement(13960..>14684)
                     /locus_tag="PW113_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2526600.1"
                     /translation="KYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDES
                     IEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANL
                     EHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHF
                     TLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKY
                     NFKVNAYELSLLYELLGPYIQK"
BASE COUNT         4482 a         3160 c         2369 g         4673 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgt
//
LOCUS       DANKHJ010000072        14533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000072 DANKHJ010000000
VERSION     DANKHJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(200..1096)
                     /locus_tag="PW113_002318"
     CDS             complement(200..1096)
                     /locus_tag="PW113_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2526601.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(1089..1769)
                     /locus_tag="PW113_002319"
     CDS             complement(1089..1769)
                     /locus_tag="PW113_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526602.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1770..2552)
                     /locus_tag="PW113_002320"
     CDS             complement(1770..2552)
                     /locus_tag="PW113_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2526603.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLQLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2545..3462)
                     /locus_tag="PW113_002321"
     CDS             complement(2545..3462)
                     /locus_tag="PW113_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2526604.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3626..3949)
                     /locus_tag="PW113_002322"
     CDS             complement(3626..3949)
                     /locus_tag="PW113_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526605.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4737..7964
                     /gene="fss3"
                     /locus_tag="PW113_002323"
     CDS             4737..7964
                     /gene="fss3"
                     /locus_tag="PW113_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2526606.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            8077..8391
                     /locus_tag="PW113_002324"
     CDS             8077..8391
                     /locus_tag="PW113_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2526607.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8404..8778
                     /locus_tag="PW113_002325"
     CDS             8404..8778
                     /locus_tag="PW113_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2526608.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8788..9123
                     /locus_tag="PW113_002326"
     CDS             8788..9123
                     /locus_tag="PW113_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2526609.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9206..10546
                     /locus_tag="PW113_002327"
     CDS             9206..10546
                     /locus_tag="PW113_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2526610.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10624..11298
                     /locus_tag="PW113_002328"
     CDS             10624..11298
                     /locus_tag="PW113_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2526611.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11291..11455
                     /locus_tag="PW113_002329"
     CDS             11291..11455
                     /locus_tag="PW113_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526612.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11531..12673
                     /gene="dcm"
                     /locus_tag="PW113_002330"
                     /pseudo
     CDS             11531..12673
                     /gene="dcm"
                     /locus_tag="PW113_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12663..12953
                     /locus_tag="PW113_002331"
     CDS             12663..12953
                     /locus_tag="PW113_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526613.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13212..14396
                     /locus_tag="PW113_002332"
     CDS             13212..14396
                     /locus_tag="PW113_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2526614.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14393..14530
                     /locus_tag="PW113_002333"
     CDS             14393..14530
                     /locus_tag="PW113_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2526615.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4648 a         2666 c         3039 g         4180 t
ORIGIN      
        1 cccatatccc tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg
       61 aaaagggggt acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata
      121 aaaacacctt ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact
      181 tcaggtaaat tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc
      241 cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa
      301 gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga
      361 agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact gttaatcgaa
      421 tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat
      481 tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat
      541 ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag
      601 agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt
      661 gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa
      721 cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca
      781 gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga
      841 cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt
      901 catccaacca tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga
      961 ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc
     1021 tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca
     1081 acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt
     1141 gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca
     1201 aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga
     1261 tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca
     1321 ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc
     1381 gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaaa
     1441 agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa
     1501 actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt
     1561 cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc
     1621 aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga
     1681 agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc atcgtcttcg
     1741 ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg gaaaataaac
     1801 attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct
     1861 tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac
     1921 tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa ggccgttccc
     1981 atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa
     2041 gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa tacttttaga
     2101 acataatttc catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa
     2161 gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg
     2221 agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc
     2281 gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga
     2341 atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat gactgtatcc
     2401 gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa
     2461 aaaagctgaa gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact
     2521 tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa
     2581 ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt ttgcttctac
     2641 caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct
     2701 agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt
     2761 cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa
     2821 ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct gtgacaattc
     2881 atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat
     2941 gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat
     3001 caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa
     3061 agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc
     3121 ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc
     3181 agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc
     3241 ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat
     3301 aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc catagatcat
     3361 tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt attttttggt
     3421 tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta
     3481 ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag
     3541 tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc tttcctatta
     3601 tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg accaaagtcg
     3661 tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc ttgagtaatt
     3721 ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa attagactgt
     3781 ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt gctaatccaa
     3841 gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct tgtatatgcg
     3901 ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt aatccttaaa
     3961 aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta ccaaataaga
     4021 ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt ctcctgccaa
     4081 ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg taaattcctt
     4141 cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa taatattgtt
     4201 tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat agaccaagaa
     4261 tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc
     4321 cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta cgattctcgt
     4381 aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa gctttacggg
     4441 ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaaa ccttgattta
     4501 taaaggtttt cccgtaaata tattttatta tatttgatta aatttgaaat gtttgtgttc
     4561 taaaatgtgt tctaatttaa aggcagtata tttcgtattt tagattaaat ttttaggctc
     4621 attcattctc aaatccattc tattcagtca ataaaaattt taaaaagact ttataaacaa
     4681 agatgttcaa gcgagcatct tatttttttg tgcttaattt gaaaggaaaa catttcatgt
     4741 ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaagcaa
     4801 ttgttggagc aattgctcct acgattactt ttgctgatga aattacccat ccacaaacgg
     4861 taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc
     4921 gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcttt aaacaagata
     4981 ttttctgtat tgaaccagga gtcccgattt acaatgagtt cactcctggc tatgagaaaa
     5041 acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag
     5101 gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt
     5161 atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg
     5221 caaaaataaa tcaagccatt gacgattatc agaagaaacc aaccttccat aatagtacgg
     5281 ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag
     5341 agttcgatga ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt
     5401 tgataatcac cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt aagaaatctg
     5461 ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg
     5521 cgattgataa acctaatacc tacacaatca aaattgatgt ggaaacggag ggttctttaa
     5581 agatcaaaaa agtcgataaa gaatcgggtg atgttgtacc aggaacggtt ttccatttag
     5641 actttggaaa aactttacct gcaaaagacg tgactactga caaagaaggc attgctacat
     5701 tggatgagat tcctcatggt acaaaagtaa ccattaccga aaaatcagta ccggcgcctt
     5761 atacgattga taccaccccc atgactacca ccattaaagc aggcgaaacc atttacgtga
     5821 cttcaaaaaa cgcacgggaa aaagggcaaa tcattctgga taaaagcgga gttgaaacag
     5881 ggagtgatct ttggaatgac aattattcct tagctggtaa tacgtttgcc attcgcaaag
     5941 acagtccaac cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa
     6001 caccaaaaga gattgctaat gcgttggaat tgggaaccta ttatgtgacc gaaactaaag
     6061 ctagtcatgg gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc
     6121 aaaccgtagc ccctgttacc agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa
     6181 ccactttgac gaaagaagac aaagatactg gggataaagc acaaggtaag gcagtctttg
     6241 agggtgctga atacaccttg tttactgcta aggatggaaa agctgttaaa tggagtgagg
     6301 cttttaaacc agagttagcg aagggaacga aaggttctga tgaaacggtc actttgactt
     6361 tagacgaaaa gaaccaagtt gccgttaaac atttagctat caacgagtat tattggcaag
     6421 aaacaaaagc gcccgaagga tatagccttg atgaaacaaa gtatcctgta tccatcaaaa
     6481 aggtggatga tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca aaagaacaag
     6541 tgattcgctt tggctttgat ttctttaaat ttgctggatc agctgcgggc actaccgaaa
     6601 ctggcttcaa tgatctgacc tttaaagtgt cgccattgga agggaccaat gaaatcacag
     6661 gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca
     6721 agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt
     6781 ttcaaaagat tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg
     6841 tgaagagtga gtatgtcttt accattaccg aacaagacca aaaacaacca attaaaacgg
     6901 tgaccgttcc ttacgagaag ctgacgaaca aagcattttc tgttagtttg aaccgtttaa
     6961 tgctctatga tttgcctgag gaagaagata gtttaacttc tcttgcgact tggaaagacg
     7021 gaaacaaaga attaacttct ctggattcta ccgagctagt tgataaattg agttacaatc
     7081 tacatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa
     7141 aagctgccca agaaaaagat gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa
     7201 cgttggcgaa caaagagaaa acaggaactt ggaaaattca gcataaatta accgccgaac
     7261 aagttttaaa taaaactatt gtcttattca actatgtgta tgaaaataag gaagcttttg
     7321 aagcaggcga taagccagta gcgaaggatg ttagcttgaa taaccaagcc caaactgtca
     7381 gttgtacagt ggaacaccat gtttctatcc aaacaaaggc ccacctagaa aacggttctc
     7441 agacctttac tcatggtgat gtggtggata tgtttgatga tgtgtcgatt actcatgatg
     7501 tactggatgg ctcaaaagaa gctttcgaaa caattctgta tgctttatta ccggatggta
     7561 caaacaaaga aatttggaaa tctggcaaga ttgattatga agtaaatgat aaagaattca
     7621 ccaaaactgt gcttgcgaaa aaagtagata ccggaaaata tccagaagga accaagttta
     7681 cttttgcgga aatcaattac gataaagatg ggaatatcaa tggaaaacac aatgaagatt
     7741 tgaaagaaaa atctcaaacc ttaacaccaa aagaagttcc aaccatacta agtacgccga
     7801 aacaaccgga aacaccaact gttccaagtg gctctcaaga atctagtccc acagtgaaga
     7861 ccttccctca aaccggggag aaaaattcca atgttttact gttcattggt tttactttga
     7921 tttttgcgac ggcgggctat tatttctgga atcgccggaa ctaaggtgat gagatgatac
     7981 aagtaaatcc taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt
     8041 tgtatccaaa actaacaatg aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca
     8101 gatatggctg aaacctttgg aaagttaagt tatgccggtg aaggagaaat tataacagaa
     8161 ggatatggac ggaatactac ggtgattggt cgcagttatc atttgtattc cagtaagcaa
     8221 cgggccgatg atattgaggt ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa
     8281 gaccaaccgc taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat
     8341 cgtgggttta ctgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag
     8401 aagatgagat taacagaagg tattgtcgta gattcaggtt taacatttgg gaagttgcgt
     8461 ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacaat tggtaatgag
     8521 gtaaaagaac gaacctataa tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt
     8581 atcccagcta atgtaccctt acgcgagttt gcttatgatg cagaggtaga gttggtcaat
     8641 ccaatcgtgg ataccgttgc caattatgtt tttcgagaag gaactaccgt taattggttc
     8701 attaaggcgg atgatttagt cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa
     8761 aatgaaagaa agaaataggt gaagattatg gaaattaatg ttgaacatat tttagattgt
     8821 ctcgatcaat atggaaaagg cgagctgact gaggaacaac tcgttaaagc attaacctct
     8881 gatgaaaaaa tgtttctgat tatacatcaa ggtttacttg gagaaaatag cgacagtgaa
     8941 gaagactctg atgtactaga ctggttagga gaagaagagt ctttctttat agttgtcgaa
     9001 gtaaatgaaa acttatgtcg gcaggcagaa tccattttgg aagaaattgg tgtggagatg
     9061 ccggatgcta ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg
     9121 tagcagtaaa tgactagcat aactttttga aattgcctaa tgaaatccta ttgggctttt
     9181 tcgattggag gtggaaacca tcaaaatgta caaaggacat cgcatacgcg caggagatca
     9241 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
     9301 cgtcttttat tttcaagagt ttagacaatt tgatatttct aagctatcac tttctactat
     9361 tgaaatcgtt tggtctataa aagatttagt atgtctgctt ggaagtctcg ctttttcagg
     9421 agctatgata ttgctttata ttcacttttt tcttgatcat tggcgaagct tgtggcatcg
     9481 acaaaagcta gctcggatga ttctagagaa ccattggtat gaagtgaaac aaactcaaag
     9541 tgaaggcttt ttcaaagatt tgaatagtag ccgaaccaga gagactatca gttacttccc
     9601 caaaatctgt tatcgaatga aagatggatt actgtctatt cgcgttcaaa tttcacttgg
     9661 gaaataccaa gatcagcttt taaaattgga aaagaagtta gaaagcggac tgtattgtga
     9721 gttagtagaa aaggaactca aagactccta tgtggagtat actttactgt atgatatgat
     9781 cgccaatcga attgggatag acgaagtagt ggcagaaaat gggactctgc gattgatgaa
     9841 aaatcaagta tgggcttatg attctttacc ccatatgtta atcgctggtg gcacgggtgg
     9901 agggaagacc tatttcctcc tcaccattat cgaagcacta ttgaagtcag atgcagaatt
     9961 gtttatcctc gatcccaaaa atgcagattt agctgattta ggtacggtga tgcctcatgt
    10021 ttattcacaa aaggaagaaa tttctgcttg tgtggaagat ttctatgaac gtatgatggc
    10081 tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt atgcctatct
    10141 tggacttcca cccaactttt taatctttga tgaatacgtg gcttatatgg agatgttaac
    10201 gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca
    10261 atctggtttc tttctgattt tggcttgcca aaggccggat gcgaaatatt taggagacgg
    10321 aatccgcgat caattcaact ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc
    10381 catgatgttt ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta
    10441 tgtggataca ggtggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta
    10501 tgatttctta gattcaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac
    10561 tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt
    10621 aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt
    10681 tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg
    10741 agtcctgggg ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat
    10801 gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaactct
    10861 agtctctttc aaaaagcatc ttcttggttg gtagagcagg catttagata tgtcttagat
    10921 catggctatt cttttattct ggatgcaact tttgcaatat taagtgctga aaaaaatatc
    10981 tcgcgggcgt tgaaaaaaca ttttcgagtg acgattttct atgtttatca agaccctaaa
    11041 atcgcttgga aatttacaca agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag
    11101 gcatttatta acgctttttt caaatcaaga gagaacattg caaaagtaaa gagtaaacat
    11161 ccagatgtat tgcttcatat cataataaaa gattatacga ataatatttc cgaagttcat
    11221 tatgctacgg ataatctaga attattttta ccactagggt atagtaggaa agatttggag
    11281 gttcaattac atgactgaga aagaacaagt aacgaaaatt gtaaagaaat acaataagag
    11341 cattgcggat ttatcggaaa atgctactgc aaaagagttc aaaacggtga ttaaatatgt
    11401 agccgatcag gccaatgaaa aacaacggaa attagttgga ttaaataaaa aatagtagga
    11461 caaattaata atgtatgagc caagagcaaa atgttcttgg cttttttctt tgcaatgaat
    11521 gaggtaatgt atgagatttt tagatttatt cgccggtatt ggtggctttc gtttaggtat
    11581 ggaacaggcg agccatcatt gtatcggttt ttgtgaaatt gataaatttg ctagacgtag
    11641 ttataaagca attcatgata caagtaagga ggtggaaatg catgacatca caagtgtatc
    11701 agatgagttt attcaatccc tcggaccagt ggacatcctt tgcggcggat ttccgtgcca
    11761 agctttttca attgcgggaa agcggcaaag attttccgat actcgaggta ctttattctt
    11821 tgaaatcgct cggttcacca ctcttctccg acctaagctt ttattccttg agaacgtccg
    11881 aggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa cgctggatgg
    11941 attggggtat gacgtggagt ggtaggtgct taactcaaag gcctacattc cccaaagccg
    12001 ggagcggata ttccttatcg gacattctcg agacgcatgt accgaacaag tatttcctat
    12061 cattggaccg tcttcaacat ctgatcaaaa catcagaaat ttggtaaata tcaatccttc
    12121 caatcgagga atgggtggac aagtctatgg ttcagatggc gtagcgccaa ccttaacagg
    12181 tgatgaagga atcaaaatcg cactgccagc aagggatgga atctctgtag caggactatt
    12241 accgggaaag tttgaacaag gtaatcgagt ttatgaagtt actggaaccg cccccacctt
    12301 gtcaacgaaa caaggtggta caaaaatcat gattcgaaaa aatgaaacag aatatcaaga
    12361 ggtgaagcca gaagacagtg tgaatctggc ttttcctaat tccacaagtc gccgaggtcg
    12421 tctgggaaaa cagagtgttc ataccttact aacaggagac caacaagcga tcgtcacgga
    12481 tcagtatcaa attcggaagc taacacctag agagtgttgg cgacttcagg gctttcccga
    12541 ttgggctttt gatcgagatt ctcaagtcaa ttcagatagt cagttgtata agcaagccgg
    12601 aaactctgta acagttccgg tcatttttga tattgccaga agattaaaag agttggatgt
    12661 acatggttta tgatgagttg attggagaaa tttattgggt gattggaaag attcagtcag
    12721 acccagaatt agaagaacaa ttgcgacggt taaaatttga tattcgcaag aatggtgtca
    12781 aagtcgctgg cgatccatat ttaatgaatg aggagacgga tgctcgaatt gaaataaatc
    12841 aagtaattgc agaatttgag cggattgcgg accaagcaaa agaaccagat attcgtcaat
    12901 acttgttaga aatgaaagca gagttggaag taaatggtat tacagatgag taacctaaac
    12961 caacacaact gactgttagg cgctgaccgc ggaggattcg cgaagcagaa atcctccgtg
    13021 gtgctgtgcc tgttcatgga cggcgttagc cgaccatgat gtctcacccc cgtatctaac
    13081 aggggggtac aaaaacacaa aaaataagta agcaaccatt aagaatgctt gttgtatcaa
    13141 agatagtaag taatttgtat aggtgttaac tcatttgaaa aagttaacac cttgaatttg
    13201 gaggtgagaa cttggcacaa cggaatttag attatcggct attaaaagac cgacgcaacg
    13261 aatacggcgt ttcacaaaat aaattggcta cggcttgtgg acttagtcgg ccgtacttaa
    13321 atcagataga aaatggtggc gtaaccgcat ctataaaaac catgaggaaa atttttgacc
    13381 aactggaaag ctttaatccc gatttgcctt tgacgttact gtttgactat gtaaggattc
    13441 gctttcccac aacggatgca cgaaaaatta ttcaagaaat tctccatttg aaatttgagt
    13501 atatgctcca tgaagattat gccttttact cctatcaaga acagtatgtc atgggtgata
    13561 ttgtggtgat gttgtctcat gaagaagata aaggtgtgct tttagaatta aaggggcgag
    13621 gttgccgaca atttgaaact tttttactcg ctcaaaagcg tagctggtac gactttttcg
    13681 aggattgtct aaaggccggt ggcgtgatga aacgcttgga cttggcaatc aatgatcgag
    13741 tggggctatt ggatatacct gagctaacga aaaaatgtca gaaggaagaa tgtatctcct
    13801 tgtttcgtac ctttaaaagt tatcgttccg gggaactttt aaaggctgat gaaaaggacg
    13861 gcatgggcaa taccttatat atcggcagtc tcaagagtga ggtttatttc tgtttgtatg
    13921 aaaaagacta tgaacaatat atcaagttag ggattccatt agaccaaact gagacgaaaa
    13981 atcgttttga gattcgactg aaaaatgacc gtgcttttca tgcgattcaa gatttgttaa
    14041 aaggtcgtag cattgagagc accacgtttt ccattatcaa tcgttacttg cgatttgcgg
    14101 ataaagtaga aggtaaaagg cgaaccaact ggcccttgaa tgaacaatgg gggcgtttta
    14161 ttggacggaa tcggaaagag attcaattaa cttccgagcc aaaaccgtat accattgaac
    14221 gaaccttgaa ttggctaggc cgacaagtcg cccctacgtg gaaaatggct aaggagttgg
    14281 atcgattgaa tcggacaacc tatatccagg atatggtaca gaatgcacgc ttatcagacc
    14341 gacataaaaa gattttggag caacaaagta tggcaattga aaatttgatt atatgaaaag
    14401 aggaatcgtc atgggaattg taaaagatat ttttttggta tttggtggca gtatgcttgg
    14461 tgttactgtg atgtgcttga tgaatgcaag tgcagcagcg gatcgagcaa tggaaaagaa
    14521 aggaaagtga caa
//
LOCUS       DANKHJ010000073        14008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000073 DANKHJ010000000
VERSION     DANKHJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(52..279)
                     /locus_tag="PW113_002334"
     CDS             complement(52..279)
                     /locus_tag="PW113_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2526616.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(601..1854)
                     /locus_tag="PW113_002335"
     CDS             complement(601..1854)
                     /locus_tag="PW113_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2526617.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            1915..2655
                     /locus_tag="PW113_002336"
     CDS             1915..2655
                     /locus_tag="PW113_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526618.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(2741..3499)
                     /locus_tag="PW113_002337"
     CDS             complement(2741..3499)
                     /locus_tag="PW113_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2526619.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(3496..4524)
                     /locus_tag="PW113_002338"
                     /pseudo
     CDS             complement(3496..4524)
                     /locus_tag="PW113_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(4544..7711)
                     /locus_tag="PW113_002339"
     CDS             complement(4544..7711)
                     /locus_tag="PW113_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526620.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(7935..8819)
                     /locus_tag="PW113_002340"
     CDS             complement(7935..8819)
                     /locus_tag="PW113_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2526621.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(8914..9351)
                     /locus_tag="PW113_002341"
     CDS             complement(8914..9351)
                     /locus_tag="PW113_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526622.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(9568..10479)
                     /locus_tag="PW113_002342"
     CDS             complement(9568..10479)
                     /locus_tag="PW113_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2526623.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(10936..11364)
                     /locus_tag="PW113_002343"
     CDS             complement(10936..11364)
                     /locus_tag="PW113_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526624.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(11392..12030)
                     /locus_tag="PW113_002344"
     CDS             complement(11392..12030)
                     /locus_tag="PW113_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526625.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(12034..14001)
                     /locus_tag="PW113_002345"
     CDS             complement(12034..14001)
                     /locus_tag="PW113_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2526626.1"
                     /translation="MVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQLIMVF
                     ENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPYSNTSF
                     RVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFEDFENS
                     YSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQVLWCLL
                     GKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVEKIASL
                     REAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGEDYHVFY
                     PVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRGIKINL
                     NYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEISEKEPK
                     FLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIKIGSLS
                     KERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYV
                     MLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFN
                     IFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         4114 a         2802 c         2300 g         4792 t
ORIGIN      
        1 cttttaaaaa aactcctgtt ttcttttatt taaaataaat gtcatagaaa atcaataatt
       61 taggtagcca ccatgtgtat gcagtagcgt gtcttcttcc cttgaggctt tttgtttttc
      121 gatacgttct tcttcccaaa cagacaacgc tactagaatc tctcttaatg attttccttc
      181 ttcggtgagc gagtactcta ctttaggcgg gacttggtta taggcttttc gatggatact
      241 ttcatcttgt tctaactctc ttaactgctg ggtcagcatt ttttttgtga tcgtagggat
      301 ttgcttgctt ctccacgcct aaaataagcg atattgctct tccctttatc tggtgtaagc
      361 aaagcagtta gcattttgac gatcgtgggt tccgcttttc acttcttatc tacttagtat
      421 catgaagtat actttcgtac taatactaag tagcggaagt gtcaactact ttgtttaact
      481 acatagcaaa agaaacagaa gggtgacaac ggtcatcacg cttctgtttc ttttgaatat
      541 gctgtgttgt tgtctctatc tccgtgtaga ctttcctcaa acttatcctt tgacagtaag
      601 tcagtaggct gatttgctct ttctagatgc cagatacatt tctacaaaaa aatagtgatt
      661 ctgttttgaa aattcgattt ttagttctgc attttcgtaa acatccaaca tttttttcgc
      721 actgtataga cctaacccgc catttttttt agaggttttt cctcttttga ccatctccga
      781 tagcgtcaaa ttcgttggat ttgccttgtt cgctatacta aaacgtgatc cctctttttc
      841 ttccagcaga ttgatcgtaa tcggactctg atcggctaca cgatgatcga atgcgttaga
      901 aataaaaata gataaacagc gaataaaaac aaccagatca atcgggattg ttttcacttc
      961 gacaggaata actacttgga tcgggatttt ctccgattct gctttttcta gaaattttaa
     1021 aaagacaccc ctgacagctg gggatacaat atttgttagc tgttgtattt gcggtttttc
     1081 aaaaatagct gcagaatgat cttctaatat ttctaaaaat tccaacgttt cttttttatt
     1141 ttcctgaaga gtaattttta atgaaaacag tagactttta taatcatgtt tgaattcctc
     1201 tagatgatgg taatattctt catttatttg atacagttga agcgattcct gatgttgtct
     1261 ttcttttact tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac
     1321 tatcgacaaa agagatagaa gcagataatt agatgagcgg aagtgaacac ttcccaatac
     1381 caagtaaatc acgccccagc ttaataaaat acaaatacct ccaaaatagt gttcagttgg
     1441 ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct atacttttta ctaatagata
     1501 gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa
     1561 ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt
     1621 actgatcaaa cagctgataa acaggttttt cgtagtaaaa taaaaaagaa acatcaataa
     1681 taagccaact aggtaaggaa agctcacttt agggatagga agtaaaagtg tcaccaccat
     1741 caaagctata caaccaaagc tgttttttac tagcagttgt ttcgaaaaga ataaatagat
     1801 acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga ccatcttatt
     1861 cctccattgt tttgtttcaa aatgtacaca tattttttta ataggagcct gcatatgaaa
     1921 aagtatttta tcatagaaga taaccagttt cacagcgaat ggatcatcag agaattggtc
     1981 aatcatcata tcgagaatat cgtgcagctc acttctatct ctcaaatcaa tcagttgatc
     2041 cagttgggca ctattgataa tcacgatgtt ttctttatcg atatcgactt gaagcaatca
     2101 ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag taatattgtt
     2161 tttttcaccg cttatacgaa ttttgcagta gagatcatca atcggaaaat ccttccgatt
     2221 ggatatatca gaaaagacag gaatttatct gaacaaattc gtcaagtgct agaacagatc
     2281 aattggaaag aacagcagat cgtaaatgtc gataaaatct ttattgatca aaaaggtgga
     2341 agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac
     2401 caagttatgg tcaaaacaac tacagaagat aaattagtga atacttcttt aaaacaattg
     2461 aagacagagt tagaaaagta tgcttacttt ttacttttaa aatcttactg tatcaactta
     2521 tcgcaattgc agaaaattga ttctgctcat tatgtgttag aatttttcaa cggtgactcc
     2581 ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac gtttccatga cttccaaaaa
     2641 actgcttcga gttgatgaat aagagctgag acgtaagcat agcagatttc agggagtggg
     2701 ccttgaatcg gctccactcc ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag
     2761 gagcatctgt taacgaccat gtgaatgttg aaacgtagtt gtctacagca taattggata
     2821 caggaacaaa taattccact gattgtctcc cttgttccac attctctcct aatgaataaa
     2881 tccatgtgcc aatcccttca tttgctcctg cagttactag atctactgcc tgatctgctg
     2941 ggatttcgat tccttcagcg ccagttccag aaacggtaga tggcattcca ctgctattag
     3001 ttgcgctatt tcgaatcacg gcgtttttga atttcacctt tgaaccagtt agtttattcg
     3061 tatcagatgt tgtaccaata aattctgttc gtttgactga taatttccaa ccggcataat
     3121 ttccgctttt atccgttact tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt
     3181 ccgttccgct ttgtaatgca gcatatactg tttttgctgt acctaacttg attgttccaa
     3241 aatcaagatc tggagcttgg tcaatcgtca gtaatccgga tgttccctga tttggttcgt
     3301 caggtgtaaa aggagtatca ggatcatttg gatcaacagg aggaactgga acaacgtctt
     3361 caggatttgc agcatctggt ttggtcacag aaatcgttcc ttctgtagtg gctgagctct
     3421 tattctcagc aaaaacactt tgtgtacata ataatagact tccagctaga acggtactta
     3481 atgcaattgt ttttttcatt tctttttcct tctttctttt aaaataaata atccagcaaa
     3541 taagccaata agaatgactc caacaagtat cagccatcgc cagtcaaatg tttctggctg
     3601 attttttaaa tcaatatcct tttcattcaa ttggtccgct gcatcatcag ttatttcaaa
     3661 atcggtatgg agattccagc gtttttcttt ccaagcaccc ttcacaaccg cttgataatg
     3721 tcccggagct aatttttttc cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc
     3781 tacatctatt ttttcttttt ttattgaaag gtaaggctct tttgtttctt ttttgtagat
     3841 ttcaaccgat gtagtcaagc cgaacatata agaagcattt ggattttgaa gattcgcctt
     3901 aatcacattt cttccatcgc tttgatccgc ataagctttt tttaatttca aatctgggga
     3961 agactctttt tcgctatttc aaatcaagat tgctttcact tgagaaaact gattgacgac
     4021 accaactgta cttttgttag aaacgttctc ttttttttct tgaaacgtga tccctcctgc
     4081 gaccacacca gccagctctt tttctggcgc tttcagtgaa agtgtctcag tgaccgcact
     4141 ttgaggagga acagttattt ctcccactgt ttttgctaag tttttcatat cataacttaa
     4201 tgtttcatca aatttacttt ttgattctcc atattcgatc acaccgtttg aattcgtcgt
     4261 agcggagtgg atcaatgtct caactgttac tgatttatct gttcggttaa agaccgtgta
     4321 ggggatttcc tgtgttttcc caggctccat ccttacattt atatatgttt tgttcttatc
     4381 aatctgatta gccggtaatt caaaatctac cgcaaaattg aattcggagg catggacagg
     4441 tgtctgccaa aggatgaatc caaggagaag gatcataagt aaagagcagt tgtttcgatg
     4501 acttttattc catattcgat tcatcaatca tcctccttga tctttattct gtcggcgcat
     4561 ctgctaatgt ccatgttatc tctgctgagt aattaccagc tatctgattt tctttggtta
     4621 agacaagttt aaacgcccga ccgttattta aatattctga ataatctgct tgcacattcg
     4681 ttgaattttg tcctctgaca atttccagtt cttcttcatt cactaattga tactcctctc
     4741 ctttcttgaa atataggaca tcctgcatag aaaatcctgc atcatttgtg aaaggctgag
     4801 ataattttat cgttaagatc cacggatttt tcgtttctcg ctgatcataa atccataatt
     4861 tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa accagttgct ttgatctgac
     4921 caaaatcgag tttggaaggg gcaagaaatg agagaccacc ttctactacg atcttcgtag
     4981 caggtgcaaa aggcggggca tcatgataaa caactggcgt atcacttgga ttgtgattcc
     5041 caatttcatc gttggtactt ggcttgttct gcacaccctt agcagtagct tctcctgctt
     5101 ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag ttgattttct gggatagaaa
     5161 gaacaaattc ccctgtatca tcaatagttg tttctgacaa aacagcttga ttttttttca
     5221 atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct gcattgatca
     5281 atgtggtact taaattagca ggttcaggag gaatcacatc gactgtcgtt ttggcagagt
     5341 ccagttctgt cacttcttgg ccacctctta cttttttagc agcaacaact tgcacttgat
     5401 cctccgtttt taatctctca tcattgggta cttgaacttc gaacaagccg ccttgttcgc
     5461 cttctcccca tgggttatat cctgcccctt cattttcatc tgtttgtcct tgcgtgagag
     5521 cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta
     5581 cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca acgacatgcc
     5641 catagatttt tttatctgca ttcgttggca ctcttaagga ttcaatcatc gggagctgat
     5701 tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca aattcatttg
     5761 ttaaacctgc actagggctt gatacgaaat tgcctaagtc ggtcccattg atcgataagt
     5821 ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt
     5881 ttcgccataa agcgatttct gaatgggcga tctttaaaga agaatctcca tttccttgga
     5941 atacccagcc gcctatgcca ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat
     6001 aggtaggaga ttctaagtcc acattcaaca catccccact gatgatgccg gtcttatctt
     6061 cccctgtttt cccgaccatg actaaataag ttttttcacc tgccgtaaaa tttaaatcaa
     6121 tatctccagt tgtatctaca gttgctccgg aatctgcgcg tatatcgata tttgattttt
     6181 ctccagtgac tgtaaaatca gctgaacctg gactttctag aaaaccagaa gcgtcataaa
     6241 attggatcgc ttgatttcct tgattgagtc tgttcacgta agatttgcca tcacctgtat
     6301 ttcttaccct caatgagcct ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac
     6361 gaactgcgct ggaacgccca ctctgcttat cgatgtcgat aactgatccg gctccttcta
     6421 cgatcaaaga aaggcctttt cctacaaaac gcatcccatt gttcccgggt tgatcgtcat
     6481 ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga ctagaatctc
     6541 cttgcaagcg aaatccgccc gtagttgctg tattgatggt gattttccca ccattcgata
     6601 cagtgacttt cgatcctgaa cctctcattt ggatgattcc gcgattgttg tcattggtaa
     6661 tattgatctt tgtatttacc gaaaattcgc ttcccgaacc actgacttca aacatgacat
     6721 taccaggtag cgtatcgact gcctctcctt catcgctaac gccatctgcc cgatcataat
     6781 caagagagac ctttgaaccg cttaagatat ggattcctgg cgctgttttt ccactaccag
     6841 tctcatctcc tgaatctaga aaaccgtaga atttccctag atacggtcca taaagttgac
     6901 taccattgat cacattcaat tgatagcttc tacaaaaata ggctttaccc ggagcggcat
     6961 tcagtccttc cttcactggt tggacatcca acacaccgga aacaccatct aatgtgacag
     7021 ttgcacgagg agcgtaaatc agtcctaatt tggacccatc atacaaatca agtgtcccgg
     7081 taaattttat ttctcccgta aaatgatatc tgcttcccca aaaatcggtc atataagcaa
     7141 tgataatggc atttccatcg atatttggtg tcaatccacc tggatgtgca gctgttcctt
     7201 taaaattctg gaactcgatc gactgaggta tctcattgat ataaatatgg tgtgtgccga
     7261 aattgtgagc gaatccacca aaatcaataa cgatatctcg tttgatctct aattccctgt
     7321 ctaaagtcaa aggctgaatc agtcggatct tagttactga ttcgtctgcg agaagctgcg
     7381 ccagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacagct cttgccagtg
     7441 ccgcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt gtcttatttt
     7501 cttcttgcct tttattagta gaagcctcac tttgagattc tgtagtttca gaagtaatcg
     7561 attcttctga aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg
     7621 ttttctctac atcggcaaaa acggatgtca ccatcaagag agggaagaca aacaaaatcg
     7681 acgcaatcag cttttttaca cattgattca ttctccacca tcctctctga cgttttgaca
     7741 attttatttt atcgttttaa tctatttcca attgtacttt catatttaaa atatgtattt
     7801 ttatggaaaa atttttctat taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga
     7861 caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg
     7921 tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca
     7981 ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat
     8041 gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct
     8101 tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc
     8161 aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact
     8221 gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact
     8281 tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa
     8341 gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata
     8401 gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc
     8461 ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat
     8521 gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct
     8581 tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca
     8641 ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt
     8701 gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca
     8761 aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc
     8821 actttctttc ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag
     8881 gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat aaaacatcca
     8941 tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa atgataggca
     9001 ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa atagcaagag
     9061 aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca gaggaatacc
     9121 aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacggcctt gaataataaa
     9181 tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa acggaaaatt gatgaatata
     9241 ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc
     9301 gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca tttatctact
     9361 tctacgtgca ttttttgata ttgttttctt tatttaagat tatacagaaa aatatctagg
     9421 gtcgctggat tctaagtaaa aaaaattttt aaaataaaaa gaactaaatc aagtagtaat
     9481 taaactactg ttgatttagt tctaataaaa agatttagat gaacagtttt attgatttaa
     9541 atagttcata ttttttattt ataccgctta tttactactt gctgtactag aatctgaagt
     9601 gaatgtactc aaaacatttt caaagtcttt gtctttgatt tttacattag cgtctttcaa
     9661 tacttcgcca attactttat tctggaaggt actgtcactt aattttgtgt ctgttgcaat
     9721 ttctttcaat tcttttttgt atttatccat gtcgttgcct ttgttttggt ttttcaccat
     9781 tttaaccaca taatattccg tagtataagt tgaagcattg gtagaagtga tcacatcaga
     9841 aacttgacca tctttcaact taaaggctgc ttctttgact tctgccggaa ccgttgtagt
     9901 agtggaatca aatttcactt ttccgccatc ttcttttgtc gtagtatcag ttgacttgtc
     9961 tttggctaat ttagagaaat cgtctccctt atcagcagct ttcttcacat cttttgcatc
    10021 atcttcactt gctacttgga taatttgagc ttccacttct ggatggaaag attcccaagc
    10081 cgtttttaaa tcttcatcgg tcaatttgat atgtttcttt agaccttctt gaaaggctag
    10141 actttgtttg atactgtctt tgaatgtttg ttctgtgtaa cctgcagatt tcaattgtga
    10201 atcaaatgaa tcacccaatt gtttctttgt ttgatcatat tgagaatcga tttctttggt
    10261 tgatacgtca tctccatatt tatcttcaaa tacttgataa acgatcaact gttgcacagc
    10321 ggaatctgct ccttgcattt ttttcaagct atccattact tcttgttcgc tgattgtacc
    10381 gcctttataa ctaacggctg ttttttcacc ggcattcgta cagcctgaca ataagccagt
    10441 gcctagtacg atggcaagtg ctaaaaatct ttttttcatt tatcaattct cctacttttc
    10501 gttttttgcg aattgctaaa aacaaaggtg ttcaagatga aaatcatcaa gaacaccttt
    10561 actaatctgc tcgatacgtc ctgaatagat ataaggctac acaatccaga aagttcgctc
    10621 agcagaataa atgtcaagta gcacatcaca ccttaatata ctacttcttt tgttaccgtt
    10681 tttcaagtct atcttgcgtt ccgtatttaa cgtaactaat tcttagaaca aatgttatcg
    10741 attttgtttt tcttcttccg ctgctgggat attcaaatca gacactttta tgttcgattt
    10801 tccagacatt tttttgttgt ttttattatg atatattttt atacaactaa aaatgaatat
    10861 attaaaacta ccaaaaataa cttttaacgc tgtttcatta tatgtttttt ctattttata
    10921 taaacttttg gatacttaat accaaccggt tggattgtac caacatgaga atgtgtcagt
    10981 tctatttcct gtcgcacttg cataggccca tactcctttt gttgcatttg ctgatcgaat
    11041 agagtttcca actttcgctt ttgcaccgtg acgattatag acacgactat agtggttaga
    11101 atgacctgtt tttctcgtac aagttgtatc actataataa acccatcttc cagagctatg
    11161 attaattgct gttctagaaa tttcccggtc tactggtaaa tttgacttaa taatattacc
    11221 tttagcatca aaatgaatct ctcctggctt taaagatttg taatagattt caccgtgaac
    11281 tgaatttgct tccacttcag atgacatagc gtttcctcca acaaaaccta tccctagtgc
    11341 acaaataata actgcttttt tcataaaacg acatcctttc ctttttgatt tttaaagcat
    11401 gatcttcttg tttcctattt tggtcagata tggatcatgc gtgactacga taactaacgt
    11461 accttgggaa gcttgttgcg ataagatttc aactacttta tccttatttc ttttatccaa
    11521 tgacccagtc ggttcatcgg ctaaaatgat tttgctattc ttcaataaga ctctcgcaca
    11581 ggcgattctt tgcttctcac caccagataa tgtgtagata ggcgtactta gcttcttgtt
    11641 tatatttact tttttcaatg cctcatactt tgctgttcgc ttatctgaaa catttaaatt
    11701 cgaaaacttc aatccaatat ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa
    11761 atcttgaaaa agaaaagata tggtatttct ttttatcaag cgcttctctc tcccagttaa
    11821 ttgtttcgtc tcttgatcga acaaatagaa ttgtcctgaa ctgaactgat cattcaatcc
    11881 taatatattc aataacgtac ttttacctgc tccactttct ccaacaacag tcacaagatc
    11941 cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt
    12001 cgcacctttt atcactatca acggctccaa ttgttaacct ccattcatca gcaataactg
    12061 acttttcttt tctgtagaca aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa
    12121 gaaaaaaata ttaaaaagat atttaggctc tctttctata agaaaaagga agattacact
    12181 tgtcgttccc catttgatga ggttcatcat gaagaaatct ttatacgttt caaagagcga
    12241 ataaccactt gaacgaaaga tagcgatttt ccgacaattg tagacaaaat atatgcataa
    12301 catgacatag ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta
    12361 atgaaatgat ctgcttaaca ctttgacagc tatctcttca gtctgagtat acggaaatga
    12421 taactgcaag ttatcacgta actgactttc ttcaatcagc tcaaataacc tttctttcga
    12481 caaagagcct atcttgatct ttaacggtgg atagatttct ccgctgagaa tatttctttc
    12541 caaatacata ctgtttttca atgtcagtat ttcaacaacc ggataatgtt cgatccaggg
    12601 gatagaagga ataaatgtat aaatgggttg attgtttttt ataaatagaa atttcggttc
    12661 tttttcactg atttcttgat aatattctgt tatttgcgcc aggtcatttt ttaaatctgt
    12721 atgtctatat cgttcaggga tcaatataac agggtgcgtt tctttttcgg ttatttggat
    12781 caaccttcca gagacatcaa tcaaaggaaa tttcttaaga taattcagat ttatttttat
    12841 ccctctgcca aactgttcat tttcttcatt caagtaatct gatacattga ctaatatact
    12901 tccgttttta ttcaaagttt catataattc ctgatctgct gctgatgcaa aacgattgga
    12961 ataaataaaa tccacgtggt ttttcccgat ggttactgga taaaatacat gatagtcctc
    13021 accagcgatt tcttttggtg gttcatggga aacagctaac tgtgaagaag aaaaaacgat
    13081 agttgctaat tcgacattgg caacggtcaa aagaagagaa aacaacaatc caaatgtaaa
    13141 aagaatgtat tggatatagt tgaacgcttc tcttaacgaa gcgatttttt caactaataa
    13201 aagagaaatc aaagagatac catatattcc gctgaacaag aaaacgtaca tctggaacaa
    13261 ataaagaaat tgctccttat aatacataaa aataaaacca ccaatgattc ctgcaattat
    13321 gaatgtaata atgattttct ttttccccaa taaacaccac aatacttgat gtaatgaaaa
    13381 gtcattattc aacaataaac tgatttcttt attcatcaat aagaaaaagg atgataacaa
    13441 aaggaataaa agaatcaatc caatagttaa atacactcga taattatata cgtctgtatt
    13501 tgaaataacc gaatacgaat agctattttc aaagtcttca aaaatattag tggcataatc
    13561 agtgtgaaat gcttgattga atcgatcagt gaatagtgaa atgaaccgtt cataggcttc
    13621 tttttctctc gtcatcaaga aaaattcacc ttcaaaatct tttattttga tcgattcttc
    13681 aatggacgtt acgcgaaagg aggtattaga atagggtacc ttctcgtgag aagatttgac
    13741 atccccttga taatacaatg tcatttcaag ctttggctgt atcttgaagt tccaagatgt
    13801 atctacttca ttggattcat ttactgtttt tttcatataa gaaagctcag tttctgatga
    13861 gacattttca aataccatga ttagctgctc atattcttct tgcccaataa aatgttgtgg
    13921 caatcgaatc ggagtataag tatctgtttg atggaaaaat tcatttgtcc gatagagtac
    13981 ctgtttattt atactaacca aggcaaaa
//
LOCUS       DANKHJ010000074        13937 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000074 DANKHJ010000000
VERSION     DANKHJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(93..1136)
                     /locus_tag="PW113_002346"
     CDS             complement(93..1136)
                     /locus_tag="PW113_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2526627.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="PW113_002347"
     CDS             complement(1513..2478)
                     /locus_tag="PW113_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2526628.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="PW113_002348"
     CDS             2712..3557
                     /locus_tag="PW113_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2526629.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="PW113_002349"
     CDS             3748..4935
                     /locus_tag="PW113_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2526630.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="PW113_002350"
     CDS             4941..5297
                     /locus_tag="PW113_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2526631.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="PW113_002351"
     CDS             5299..5637
                     /locus_tag="PW113_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2526632.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="PW113_002352"
     CDS             6032..7066
                     /locus_tag="PW113_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2526633.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="PW113_002353"
     CDS             7059..7745
                     /locus_tag="PW113_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526634.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="PW113_002354"
     CDS             7769..8617
                     /locus_tag="PW113_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2526635.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="PW113_002355"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="PW113_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2526636.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="PW113_002356"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="PW113_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2526637.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="PW113_002357"
     CDS             10883..12118
                     /locus_tag="PW113_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2526638.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /locus_tag="PW113_002358"
     CDS             12105..12575
                     /locus_tag="PW113_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2526639.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..>13937
                     /gene="sufB"
                     /locus_tag="PW113_002359"
     CDS             12580..>13937
                     /gene="sufB"
                     /locus_tag="PW113_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2526640.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NR"
BASE COUNT         4626 a         2629 c         3035 g         3647 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgatt
//
LOCUS       DANKHJ010000075        13245 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000075 DANKHJ010000000
VERSION     DANKHJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..888
                     /locus_tag="PW113_002360"
     CDS             <1..888
                     /locus_tag="PW113_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2526641.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(930..1622)
                     /locus_tag="PW113_002361"
     CDS             complement(930..1622)
                     /locus_tag="PW113_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2526642.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1617..1883
                     /locus_tag="PW113_002362"
     CDS             1617..1883
                     /locus_tag="PW113_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2526643.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(2059..2628)
                     /locus_tag="PW113_002363"
     CDS             complement(2059..2628)
                     /locus_tag="PW113_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2526644.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2702..3990)
                     /locus_tag="PW113_002364"
                     /pseudo
     CDS             complement(2702..3990)
                     /locus_tag="PW113_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4344..5192
                     /locus_tag="PW113_002365"
     CDS             4344..5192
                     /locus_tag="PW113_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2526645.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5337..6278
                     /locus_tag="PW113_002366"
     CDS             5337..6278
                     /locus_tag="PW113_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2526646.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6268..8439
                     /locus_tag="PW113_002367"
                     /pseudo
     CDS             6268..8439
                     /locus_tag="PW113_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8551..10722)
                     /gene="copB"
                     /locus_tag="PW113_002368"
     CDS             complement(8551..10722)
                     /gene="copB"
                     /locus_tag="PW113_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2526647.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(10742..12928)
                     /locus_tag="PW113_002369"
     CDS             complement(10742..12928)
                     /locus_tag="PW113_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2526648.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12928..13137)
                     /gene="copZ"
                     /locus_tag="PW113_002370"
     CDS             complement(12928..13137)
                     /gene="copZ"
                     /locus_tag="PW113_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2526649.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13150..>13245)
                     /locus_tag="PW113_002371"
     CDS             complement(13150..>13245)
                     /locus_tag="PW113_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2526650.1"
                     /translation="NEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
BASE COUNT         3872 a         2701 c         2360 g         4312 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acaaagaggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc
      961 ttgtcatgaa aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc
     1021 atcatgacgt atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca
     1081 acatttccta aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt
     1141 actctagcta gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa
     1201 ttagcaatgt gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca
     1261 atgtgggcgt aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga
     1321 tcaattactg gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa
     1381 tcataggcta gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta
     1441 tgatcgcccc aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
     1501 ggagtgtcta atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg
     1561 tatactttat agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc
     1621 acaggacttt tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa
     1681 ggaaaacacc aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt
     1741 tagaagatat caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc
     1801 atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc
     1861 aaataaaaaa gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact
     1921 aattttattt ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc
     1981 ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga
     2041 agtgcgaaca tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc
     2101 catcaattga gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg
     2161 cggttttctt cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc
     2221 cacgactccg ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc
     2281 tagatttttt gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag
     2341 ctcttccatc ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt
     2401 tatttctttt ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg
     2461 caagagcgtt ttgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa
     2521 tatatatacg ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt
     2581 cacttctgtg atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc
     2641 tttactaatc gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt
     2701 tttatcggtt tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg
     2761 aaggatggta cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg
     2821 tgatcactag atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc
     2881 gttttcgatg tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt
     2941 catagcgaaa aaaaagacga ttttcacttc aggagggtgg acatctaaat accctcgttc
     3001 ctggagtgtt ttcagtaaaa gcatccccca ctctactgcg gggtgtgcaa gtgcttgttg
     3061 gatcgctagc cgaaattgat atagtttatc tttggaattg ggaaattttt ctaatgtttc
     3121 ctgataataa cgaacttctt ctatcagccg gtcatggata ccaattacgt aacgaagcca
     3181 ttcttggtaa agtactttta tcagctttga tttgctttcg ctctcaaatt cgctattcag
     3241 ccaaaacagc gaatcaagct tttcagtcac aatcgtcaat ttccgatagt cttgtgtctc
     3301 ttctatccgt ttccaccgct tttcagtaat ctggttacag ctcaagcgca aaattgctag
     3361 ataatttttt tcttttattg gcaaaatctg ctcctctcta aatagcaatt caggaaacaa
     3421 ttcaaagaat agtgttcgac tgtctcctaa aatctcgatt tctgccggat cctttctttg
     3481 cttcaataat tggatgaaca tctcagaaaa ggactgctct ccatcatctg caaaaaaatg
     3541 cggaaaatct ttcaaaaagt aaaacagcag atatcttact tttctttctg aaccaaagat
     3601 ctgtgctttt ttataagaaa aagaaaggtg gtactcgcca aaccactttc tcaatcgctc
     3661 ttgcgtagaa cgatcgtact tctgctcgta aggttcattt aatagcagat ttttcagtaa
     3721 acaaaacggg ggtgattgga tcagaaaaac aaacaagaga taaggttcgc tatatccttg
     3781 atcataaact aactgaacgg ttttcttatg aagaatcagc tgaatgggac agtcgctttc
     3841 cgccaaacgt ttcagatgcc gatccactgt tttaacatcg attttcagtt cttcagctaa
     3901 atcagctttt cgccagatca aatctttcat tagattgatt agtgaaaata aattctgtgc
     3961 attttttgat aatagttttt gccatctcat cgatcatttc tcacctgatt tctaaacata
     4021 ttgtttgatc aaaatgagtt cactcctaaa aagataaaaa actccctttc tatttattta
     4081 aatacgcaaa tcttcctcat tattttttat tttactcaat ttaatcgtat tctcatttca
     4141 aaacgaatgt actttaaaaa aacataataa gatcactttg cttgtaggat gtattccctt
     4201 ccattacaat gaaataaata gcgattttat gagaaggtga tgtgaaaatg gaagaaaaaa
     4261 tagaagaatc caaacaaaaa atcgatcagt tgatataaga attagaaaaa gcggtcatcg
     4321 gcaaacgttc aactatccaa ttgatgatcg tggccctatt agcaggtggt catgtcttat
     4381 tcgaagatat tcccggtgta ggtaaaacgt tactggtcaa agcactagca aaagctgtac
     4441 agggaacctt cagcagagtc caatgtactc ctgatctgct gcctagtgat attcttggtt
     4501 tttccgtata caattcacaa tcaaaagagt ttgaatttcg tccgggaccg atctttacca
     4561 cgatcttgtt ggcagatgag atcaatcgta cgactcctcg tactcagtca gcacttttag
     4621 aagcaatggc cgagaatcat gcaacgatcg acaacaatac ataccctcta gacaatcatt
     4681 tttttgtctt agctacgcaa aatccaatcg agtacgaagg aacatatcct ttgccggaag
     4741 cacagctcga tcgtttttta ttccgactac aaatcggtta tccttctttt gatgacgaac
     4801 ttttgctact tttggataaa ttcgaaaaag tgttggataa cttaaatgaa gtattgaaca
     4861 gttatgaaat agaagaaata aaaacaaatg tggcgaatgt ctttgtcagt cccgaagtgg
     4921 catcttatgc cttgcagtta gtgtcagcaa caagaagtca tgctgccatc cagcttggca
     4981 tcagtccacg aggaagcctt tcctttattc aggcagcaaa agcttatgct ttaatccatg
     5041 ggagaaatta cgtcacgccc aaagatttgc aggatttagt tccttacgtg ttttcccatc
     5101 gcctgatttt ttacgatcgt tcattgaagg aagacgaaaa gaaacaaatc attcatactc
     5161 ttgttgcaca aattcctatt cctgtgaggt agattgaggt agacaatgga aaaaatcaaa
     5221 gattatttca ttaattttgt gttatttctg gtatatttca tcattgtgct ttttgctctt
     5281 gtattcaaca attctttagg ctggttcttg ttggcctttt ttactcttta tgtatcatgg
     5341 agcttgattt cattaagcag ctttctaaag aaactttcgg ttacttgtac attgcagcca
     5401 ctcgtttatc gcaaaaaaag tatgcaggtt attttttcca tagcaaaaaa gagaactttt
     5461 tctttacctc tccctcgttt acaaatcggc ttcccagcaa tctttgcaga aaaaaacaag
     5521 agattttgat tcttacaaat aaaccgcaag aagtccaatt gctttggcag ccaaaagaaa
     5581 gaggatgcca tgaaacatta actgtcattt ttacagccta tgatagtcta ggacttttca
     5641 gaaaaagtgc aaaaagagag atctcttttc ctgtgactgt tctgcctgct ttttgtaaga
     5701 ttcaagcaga aaaattacag agagttctgg aaaaaaaaca gcagctgaac gcgtaccaaa
     5761 gaggtcttga ttttcgggaa catcgcgctt atcgtccagg tgattctttc aaccgaatcg
     5821 attggaaatt gtctgctcat actcaagagt ggttataccg tgaatacgat tatcaagaag
     5881 aagaatgttc tcctcttttt tgcttttggg gttcgcccag tccaaaattt gaacctctgc
     5941 tagacttgta tttttcttta tggcatttga gaaaagaaaa gcagccggaa cttctgatat
     6001 taggcaaccg cgcctttcat ggcaaagacc ccggtcatac ctattttgct tctttggaag
     6061 ctggcgatga gaaagctttt ttcgcacact tgaaaaaata tgcaaaaaaa caaatcgtcc
     6121 tctttgttcc tgagaattct cctgaagtct cagaagctat tgaaacattg tcaaaagatc
     6181 gaacggctta tcttgtttat ttcgtagaaa aacaactcat ggtccaagaa aaagacaaga
     6241 tatgttcatt gccaggaggt gaatggattg acgattaaac gggaaaaatg gctgcgtggc
     6301 gtttactcct ttgtgatact aagtgtaggt gctccgatat ttgtagtagc taatcaattg
     6361 caaaacagct accttcttat cctaactatt ggtgtcctat gccttattgt ctgctgtatt
     6421 cctaaaaaat gggtacgttt gctgctttct ttgccagtaa tgataggttg tttgttttta
     6481 tattttcctt cgaagacatt ttccatatta tggatcttac agtttttttc gtcagttaca
     6541 gaggaattct tccatctatc ccaaggtaaa ctttattatt tgccagaaaa gacagctttt
     6601 tttctcataa tgttgctgat ctatttattt attacattga ccgtggatta cgactattgg
     6661 tacgcaccct ttctttcttt gatggcttac ttcatgatgc ttaccgtttt tcggaaaaaa
     6721 gatttactgt acgaaatgat tgcggtagta actgttttgt tcgtgtatct ggcagcaagg
     6781 caatttttct ccatccggtt gttgtcggga aacactcgat tccagtcatt gactacagtt
     6841 ctgcttattt gtttcttaac ttttagcgct atcttgcctt tatatcttcc gaaagtacac
     6901 caatctctcg aggaaacgag tgaaccgatc cgagaatatc ttaatgatga agggctttat
     6961 gacacactcc atgagtatca attaaccgga agtgcaagaa caggcttcag tgagaatgat
     7021 gaacagcttg gtggaccttt atatgataat ggcgaagttg tttttacagc gaaccaaaaa
     7081 ggaaatggct attggaagat cgaaaataaa aatcattaca ccggttctgg atggatagaa
     7141 gatcaggacg cacccatcca accaatccaa gaattccctg ttgacatcac gagctaccaa
     7201 accgttatag aagacaaaga aaacattaca ttgaccctaa aaaaacaagc tttcttacct
     7261 tatccttatg gaaatactac cttgactgat ttttcattag ctagtggaca aacgctttac
     7321 tatttgcctg aaagccagcg attcattcct gtcccgaatg aatcgacacg ttctgcaact
     7381 ttacaaacag aaaggttagt ttttacgaga gaagaattaa aaagcagtca gttaaaagct
     7441 gatgatttag aaaatcagca gacaaatctt caattgccaa aaagtttgcc taaacgtatt
     7501 gaggacttgg cggtcgaatt gaccaaaaat gaagcaactt taattcgtaa agtcgaagct
     7561 gtctaacagt atttaaaaac aagcggaaat ttccgctatt ccaaaacgga taccgggcat
     7621 actccgaaag aacacgatta tgtggattat ttcctctttg attcaaaagt cggctattgt
     7681 gacaactttt caacttctat ggtcgtcctt cttcgaacac ttggtatccc agctcgctgg
     7741 acaaaaggtt ttacaaatgg gctcagaaca ggcacagata atcaagggaa tgaggaatac
     7801 agcattctca atagtcatgc tcattcttgg ccagaggtat actttcctgg atttggctgg
     7861 cttccatttg aaccaacccc ttcatttgcc aatccaatgg gttcacaacc agttgctgaa
     7921 acaaccgaaa ccgtccagcc tgctatcgaa caaccaaatg aaagcgaaaa tatggcagaa
     7981 actatttcca gtgagagatc tgaggtcatt ccatcagaag aaacaacact cacaccagct
     8041 tataaaatcg tcttattcag ccttggagtg tgtttatccg tcttgttgtt ctttctattg
     8101 ttattcagaa aatctctgcg ctggacaagt tatcttatct atcttcattt ttttgcttct
     8161 actaatacga ttgcatatca aaaacttctt aaccctttgg aaacattggt accgcgcaaa
     8221 cccgcagaat ctttgcagga atatagcctg cgaatagaac aagaattcaa gctagatctg
     8281 cgtttcattc gattaactac tcaatatgaa gcagaaatat atggacgaca agcagatccg
     8341 gtaaaattcg ataaaaaaac ctatttagaa ataacgcgta taataagaaa aacggtatac
     8401 aaaaaaattc tgacaaaagg gagccgcttc aaaaaataag ataaaatttc tggagaatct
     8461 acttctggaa tgccgagaac cagctgattg agtatcgatt actcgaaaaa aaggttgtga
     8521 cacgtttttt gtcacaacct tttttcatta ttatttgatt ttcaatgtca aagcatttag
     8581 agctacaact accgtactta acgacatcaa gacagcacca actgctggac ttaatatgat
     8641 accaattggc gcaagaattc cggctgctag cggaattgca agaatattgt aacctgcccc
     8701 ccaccagagg ttctggatca ttttctttcg cgtttgcttg gctagatcaa gaaaatgcaa
     8761 aatatctttt ggatcgctgt ttgtcaatac gacatccgca gaatcgattg cgatatctgt
     8821 ccctgcaccg attgccatcc cgattgaagc acgggctaaa cttggcgcat cattgatccc
     8881 atcacccacc atgattactt ttttcccttg gtcaaggtat ttctggacaa cagcttcttt
     8941 atcatctggt aataaaccgc cataatattc ttcgatccct aaataatctg ctactgcttg
     9001 ggcagcgttt ttattgtcac cagtaagcat gacaggagtg atttttcttt ctttcaatgt
     9061 tcggataaat tcttttgctt ctgtttttac tacgtcacct aaagcaacga ttgcagctag
     9121 atgattttct aaaatcagga agctgatggt atttccttgt tcttggtaag gtttcaataa
     9181 tgcttgttct acgtttaatc cgagacgctc aacttctttt tcattaacta ttttcacttg
     9241 ttgttttttg actgttgcct ctagcccgac tcctgaaaga ttttttagat tttcagcttg
     9301 ataaggcttg atttcatgtt ctttcaaata attcataatg ccaacagcca atggatgatt
     9361 ggcattcgct tccaaggctc ctagatattg aaggatttca tttttattga acgcatcatc
     9421 aagtacttcg acacccgtta cagtaaattt tccttctgtt agtgttcctg ttttgtccag
     9481 catgatcaca tctagatcat tggcttgctc cagtgcattg cggtttttca aaagtaatcc
     9541 gtttttagct gctattgatg tggagcgagc aacaactaat gggatagcta aacctagagc
     9601 atgaggacag gcgatgataa agactgttac catacgttct aatgcattag gaagatctgt
     9661 caagaacaac caggcgatga atgccaggat acccacaatt aatgcgacat aaaacagcca
     9721 ttttgccact ttgtcagata atgactctag cttggatttt tctccttgtg cttttcgcac
     9781 catttccata accttcgcta gataaccatt ttcaccagtt ccagttactt cgatttcgat
     9841 cgttccatcc ccattgatag aaccgccaat cactttgtca ttggcttgtt ttttgattcc
     9901 tcttgattcg ccagtcacag ctgattcgtc aacaattgtt tcacctttca agattacacc
     9961 atctgttggc attttatcac cagaccggac gatcaaacga tcaccttcgt ttacttcttt
    10021 caatgaaact gtctcttctt ttccatcttt cgtcaagcgt ttcactgatt ctggcagtaa
    10081 ttctgctagt ttttgtaaag cattgctggc attcgacaca gcattcattt caatccagtg
    10141 acctagtagc ataataacga tcaaagtagc caattcccag aagaaatcca tgacgtgtgt
    10201 atgtggattg agcagatttg cgataaacga atagacacta taaatatatg caactgtaat
    10261 ccccatcgcg atcagtgtca tcatagcagg acttttttgt ttcaattcca tcttagctcc
    10321 acttaaaaat ggctgaccgc catagataaa tagtatagtt gctaagatca atacgagcca
    10381 atcagaacca ggaaacgtta cttgaaatgg aaattccatt cccatcattg gtgaaaaaag
    10441 aatgattggg atagctagga taagcgatag ccaaaatttt tgtttgaaat tccccatgtg
    10501 catggaatga tccatgccgc cgtgttcgtg atccatgtgc atcgaatggt ccatttgttt
    10561 gctgtttgct atatctccat ggttatgtcc catgtgcatg gaatgatcca tctgactatg
    10621 gtcatgtttc atctccatct gtttttcatg agattctgta ctttctttgt ttactacttc
    10681 ttttttatgc tcatgttttt tctccatatc gttcccattc atttttactc catcccttct
    10741 cttacttttt catgtgtcga tttaagctca gggagttcaa caatacactg acggaactaa
    10801 acgccatcgc tcctcctgca atgattggat tcaaaaagcc tagtgcggca aaaggaatac
    10861 caatcgtatt atagacaaaa gcccagaaaa gattttgttt gatttttctc aatgtcaacc
    10921 tagacaactc gattgtttgt gcaatggatg ccaaactgct attcatcagc gtcacatcgg
    10981 ctgtttccat cgcaatgtcc gttccacttc ccattgccat accgacatca gctaatgcta
    11041 aggccggtgc atcattgatt ccatcgcctg ccataccgac ttttttgcca tctttctgaa
    11101 gtttttctac gtaggctgct ttatcctctg gaagtacttc agcaaagata tgctttggat
    11161 caataccaac ttgtttacca atcgtttcgg ctgccagttt gttgtctcct gtcagcataa
    11221 aaacatctaa tcctttattc tggagctgct tgattgcttg tttggcttct agtttgattt
    11281 gatcagccac ggcaatccac ccgatcactt gcttttcatc agctaaaaac atgactgttt
    11341 ttccctgctg ttctaattct aacgcgtgtt cttggtattc atcaaatgat aggttcaatt
    11401 caattaatcg tttacgtgta cccgcaaaat agcgtactcc attaatcgta ccgctgatcc
    11461 cagcacctgg gtgggctgta aaatcaatca tatcgtatgt tgctgcttct tgcagttttc
    11521 cgtattccac gattgcttta cctagtggat gttcagaagc ttgttctaaa gtataaaaaa
    11581 tagataggac ttcttttgaa ccgactagat ctgtaacttt gggctttcct tctgtaatcg
    11641 ttccagtttt atccaaaaca atgctgtcta agtgagcagc tgcttctaat gcttctccgc
    11701 ctttaattaa aattccattt cgtgcaccaa cacccgttcc taccataata gctgttgggg
    11761 tcgctaaacc taatgcacat ggacacgcaa tgactaatac agagacacta tggatcaatg
    11821 cgagttgcca atcaccagtg atcaaacctg tagctatgag cgtgataaac gcgatgacta
    11881 atacgatcgg aacaaaaatc cctgagatcc gatctgcgat tttttggatt ggggctttgc
    11941 tcccttgagc atcttccacc atttgaatga tttgcgctaa tactgtctgc ttgccgactt
    12001 gtgtgacttc catgtgtagc agaccattcg tattgattgt cccgccaaaa agcgtgtcat
    12061 ccggattctt ttctacagga agattttcac cagtaagcat actttcatct atcgcactgc
    12121 ttcctgaaat aattcgacca tctgctggaa tctgctcacc aggacggatg actagttgat
    12181 caccaacgac tacttcttca atcgctaacg tttcttcttt cccgtttctt atcacttggg
    12241 cggtttttgt ttgaagagac atcaattgtt tgattgcatt acctgttttc gtttttgctg
    12301 tatgttccaa atatttgccg agcaaaatca gcgtgatgat catactgctg ctttcaaaat
    12361 aaagatcttg ggggtgtcca cggaaaaatc cgttgtaaat gctcaatgca aaggcagccg
    12421 atgttccaat tgctaccaat acatccatat ttggtgcttt cgtcttgatt gcatggtacg
    12481 cacctttata aaaacgagca ccgacataaa attggacagg agcagacagc actaactgga
    12541 cgattggaaa atgaaaaaag tgaacaatcg ccgcatggct tcctaacatc atagcaatca
    12601 ttgacaacat caacggcaaa gtcaaaaccg tactcaatat aagatcgaat agcatttttt
    12661 tcaaatacgc ttgcttttct tctgcaattt tttgtttgtg ggcttcatcg tacaaaattg
    12721 caccataacc gatgttttct acgctttgga ttaatttttc agtggtcgtc ccttcatatt
    12781 tgacacttgc tttttcagtg gccaagttga ctgtggcatt gaccacacca ggttgtccgt
    12841 tcagctcttt ttcgatccta gcagaacaat ttgcacaagt catacctgta atgacgaacg
    12901 tatccatttt ggtttctgct gttgccatca aatcacctct gctttataac ccaatccatt
    12961 gattgcttga caaatctttt cggattgaac ttttgtttca tcaaacttga ccgtaccgtt
    13021 tgcttttttc aaatttactt tcactttttg cacgccgtca agtgcgctta ctgtttcttc
    13081 tactcttgct gcacaatgat tgcagctcat accttcgatt gaaaattgtt gtttcatttt
    13141 ttcttcctcc tattgatgtt gtttacattc acattgacct gggatacagt tacattcgat
    13201 cgtatctact ggttgctttt gttccagttg tttcaaaatc tcatt
//
LOCUS       DANKHJ010000076        13220 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000076 DANKHJ010000000
VERSION     DANKHJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            152..1075
                     /locus_tag="PW113_002372"
     CDS             152..1075
                     /locus_tag="PW113_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2526651.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="PW113_002373"
     CDS             1081..2334
                     /locus_tag="PW113_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2526652.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="PW113_002374"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="PW113_002374"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2526653.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4749..5702)
                     /gene="menA"
                     /locus_tag="PW113_002375"
     CDS             complement(4749..5702)
                     /gene="menA"
                     /locus_tag="PW113_002375"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2526654.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5715..6818)
                     /locus_tag="PW113_002376"
     CDS             complement(5715..6818)
                     /locus_tag="PW113_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2526655.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6908..7864)
                     /locus_tag="PW113_002377"
     CDS             complement(6908..7864)
                     /locus_tag="PW113_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2526656.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8145..10079
                     /locus_tag="PW113_002378"
     CDS             8145..10079
                     /locus_tag="PW113_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2526657.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10536..10952
                     /locus_tag="PW113_002379"
     CDS             10536..10952
                     /locus_tag="PW113_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2526658.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10966..11532
                     /locus_tag="PW113_002380"
     CDS             10966..11532
                     /locus_tag="PW113_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2526659.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11555..12541
                     /locus_tag="PW113_002381"
     CDS             11555..12541
                     /locus_tag="PW113_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2526660.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12696..>13220)
                     /gene="scm"
                     /locus_tag="PW113_002382"
     CDS             complement(12696..>13220)
                     /gene="scm"
                     /locus_tag="PW113_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2526661.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         4118 a         2400 c         2725 g         3977 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattttacaa cctctgataa
     4381 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc
     4441 cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca acctctgata
     4501 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtggaaag
     4561 ccattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac aacctcctga
     4621 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatataag
     4681 gagctatttt tatttggctg ttcttattac ttgatgcagg gcagtttttt cacaacctct
     4741 tcatgctgtt aaatggcttg cataatgatt gttagtaata gccccaatac ataactggca
     4801 ttgaataaga ccatattttt gatggaatag ctgaaactga taggctgggg taacgtttgc
     4861 ttgaattgat ccaaatgctt cttcacaatt ggcaaggtca agaaggtgac caagatcggc
     4921 cattcataga taccgctcaa caaccccatt aaaatcacga aatagcaagc atacatcaat
     4981 agctggaata agactgttcc cgcaggacgt ccgatataaa aaactagtgt atagcgatga
     5041 ttttcaatat cacggtcaag atcccgcaaa ttattagcca acatgatatt ggcaatcgta
     5101 aacaccaagg gtaaggaagc taatacgatt gctgctatag cccaaagatt tcctaccaaa
     5161 gcaaattgtc cagatgaaaa gttgactgtc agatcataga catctgaacc gaatgtattg
     5221 acataaacga ccatggcaaa aattccaagg cccatagtca ccccactgaa aatttcccct
     5281 aaaggcattc gagataaagg aatcggtccg aatgtataga atactccaat aaaacaacag
     5341 ataaatccca tcaaaagtaa aagccaaccc gtattgatcg ttagaataac gccgataaca
     5401 aaaacaaatg caatcatagc taaaatcatg tttcgaatca atacaggaga tagatttctg
     5461 cggccaatga cattttcttc atacttatat acttcagaat gagccttttc ataatccatg
     5521 taattattga tagcggtagt cgctaaatca aaaactagca ttccaataaa aaataaaaaa
     5581 gtgtttagcc aatgtatctc atgaaaaaaa gcgatagaga ataatacccc taccgcaaaa
     5641 ggaaatacac tagccacctt tgttcgtatt tcaactactt ctaaaaagat ttttagtgtc
     5701 atggttcgta ctccttattt tagttcagaa cggtcaccca tcgtgtaatg ggagtcctcc
     5761 gataatttga aggtgtcttt gattggatcg gtcacatata ctttatcgtc ttttgtcacg
     5821 aagatcgctt ccgtcccgtt attcaattcg ctttcgacgt attccagtcc ttttttcaca
     5881 cccattgaaa agactgctgt agacaacccg tcaccatcaa ttgatttatc tgttatgatc
     5941 gtgacacttg cgatatcatt gtcgaacgga taacccgttt ttggatcaaa caaatgatgg
     6001 tattttttgc catctacttc taaataacgc tcgtaaattc cagaggtaac taatgttttg
     6061 ttccgttctt taatagaacc taaaacagat ccgcgagcca tgttcggatc ttggatgccg
     6121 actgtccagt cttggttttc gcctcttggg ctgtggccta agacatagac attaccgccc
     6181 aagtcaacga tggcagtggt cactccttgt tttttcaaga ctttcactac ttcgtccgta
     6241 atgtaacctt tggcgattgc acctaaatcg atgatcattc ctttttcttt tagataaaca
     6301 gttttttctt tgtcatttag ttcgatctta tgataatcca ctaatttcaa tgcctgatcg
     6361 atttcttctt gggaaggttt tctggcgtca tcaaaaccga ttcgccataa ttgtgtgatt
     6421 gcaccaatcg ccatatcgaa tccgccttga gaatcttggc tatattcgta ggcccgcttg
     6481 actaacgtgt agacgtcatc agaaactttt actggtttga tcccagcttg ctcattcact
     6541 tcatcgatct cagaaccttt ttgattgatc gtgattttat caccaagttc ttttactcga
     6601 tcgaaggcag gtttcaatgc tgattctttg ccttcatcat acacacggat acgaacgtaa
     6661 gtccctaaca agaactgttc atcagaatat ggatctttca atatcttggt agctgctgaa
     6721 ctttcagtgg ttgttttatt tgctgagttg ctgcagccag ttagcaccat aaaagagatg
     6781 cccagtgtga ttaaggaaaa tagtagtttt ttccgcatct ttttcctctc ttcattttga
     6841 aaggtgagac gactgcctaa tctggctatc gtcccaccct ccaactttta ttagtatgat
     6901 tatgtgatta tttttctgtt acgatattat ctacttcgat tgtttgagtg tcgccttttt
     6961 cagctgcatt cactaattgt tgtgcgtaca tgatgaatga atgtgagctg tgtgtagcac
     7021 ctgtaacaac ttctacatta gcaggtgagc catcttcttc gcccattgct ttgacgaatt
     7081 cgtcgttcag agtagggatg taagtttttg gttctgtacc gctttttgct ttcatatttt
     7141 cattatattc tgtatcatct tgttttgatt taccgtcttt gtcgatataa tcaaagtttg
     7201 attcagttac tttgccgcca gcaactgtca tttcgaattg cacacggtag ccgttagagt
     7261 agttcttttc ttcaagcttg taagtgccgt ctttcaaatc agcaccattg tcgatttcga
     7321 ttgtatctgt gttaccagct tgagcagctt ggatcaattg ttgtgcgtag ttttggaagc
     7381 tttcagatga gtgagtagct ccagttacga cttccacacc gcttgcgctt tgtgctttta
     7441 agaattggtc attcaactca ggaatgtatt tttctgggtt tgtgccagat tttgcttcca
     7501 tgtttttgtt gtattctgca tcttttgttt ttgattcgcc gttttcattg acgttatcat
     7561 attttgattc agtgattttg ccgtctttta caaccatttc aaagactgca cggtaaccat
     7621 ttgaataatt tttttcttct aatttatatg tgccgtcttg caaatctccg ccagctacaa
     7681 cttttgcaga tgattctgtt gtgctttttg cagtcgaaga ttgtgctgta gatgattcag
     7741 aagaagagtt tgagcttccg ttgtcatctg cgccgcaagc gcctaacact agagctgaaa
     7801 atgctaatac ggcaaatcca gttacaaaac gttttttctt cattttaata ccccaccatt
     7861 ccatttctag tccacaattt ttctttgtga ttcattatac attctaatct ttttagccta
     7921 taagtcaact atttttgata aataacccat gtaatatttc aaacgttttc tttcagcaaa
     7981 tttcgtgaaa aaacgcttta ttttaaaatt tgaaaaaaat ccaaaaggag ttagacaaaa
     8041 gaacagaaca tctatataat atatggagat tgtgtaataa gtatcaaact aaaagaatta
     8101 tcatatattc attatataga tagtctataa ggagcggatg atcaatggca aagcaaaata
     8161 ttgtcgttgt aggagcagga tatgccggtg tttcagctac aaaatacttg gcaaagaaac
     8221 tgaaaaaaga agatgtagca attactctga tcgatcgtca ttcgtaccat actatgatga
     8281 ccgagttgca tgaagtagct ggcggacgtg tagaaccaga agcaatccaa tacgatctgc
     8341 agcatttgtt tgctcgtcaa aagaatgtac aacttgtcac agacactgta atcggtatcg
     8401 ataaagaacg aaaaatcgtc aagaccaaat taggttccta tccttttgat cagctgatcc
     8461 ttggaatggg cggagagccg aatgactttg gtacacctgg tgtcaaagca cacggattca
     8521 ccctttggtc atttgaagat tctgtaagga ttcgggaaca tattgagaag acagtagcaa
     8581 aagctgcatt ggaaccagat gatgaactgc gtaaagccat gctgactttc gttgtctgtg
     8641 gttctggatt taccggtatt gaaatgattg gggaactgat cgactggaaa aatcgcctag
     8701 ccaaagatta taaattagat ccaaacgaaa tcacactgat ggtcgtagag gcaatgccga
     8761 cgatcttgaa tatgttggat cgaaatgatg ctgcaaaagc tgaacgctat ttgcgtaagc
     8821 acaacgtgga attattattg aatgcaccaa tcgtagaagt ggctgctgat catatcaaat
     8881 tgaaagacgg acgcaacatt ccgactcaca cactgatttg gacagctggt gtcaaaggaa
     8941 cttctgacgc tgctgacttt ggattagaat cagcacgcgg acagcggttg atcgccaatg
     9001 aatatatgca ggcaaaaggc tacgaagata aaaacatcta tatcattggt gatttagtct
     9061 tttatgaaga attcccagga acaccgacac cgcagatcgt tcaagctgct gaacaaacag
     9121 gacacacagc cgcagcgaat atcgtggctt cgatcaaagg cggagaaaag cataaattta
     9181 aaggaaacta tcaaggattt atggtttcta ttggtgccaa atggggggta gctaacctat
     9241 ttgataagat ccatttaagc ggatttttag ctatcgtaat gaaacatatc gtgaatttga
     9301 aatacttctt tgatattcgt tctggttatt atatgttcca atatttaatg catgagtttt
     9361 tccatatcaa agatgatcgc aacgtgactc gaggacattc ttctcgttac ggcaatgtct
     9421 tatggagtgt gccacttcgt attttctatg gtttagtttg gttcgttgaa gcttccaaaa
     9481 aaatcattgg gaatggtgaa gtgttcaaac caagtacttg gtttggtgaa ggatcatggt
     9541 tcactggaaa cagtgtgttc ccttggacat gggaatacaa tgtaacagat gttgccagtg
     9601 gtgcttctgc tgctggtgat gcaaccacag ctgccagcgg agcaggggca agtgccggag
     9661 aagcagcgac tcaagctgca cattttggtt tgagttatgc atacggtgaa gaaccaatgg
     9721 ctgtgattga gaagtcacca gactggttca caaagatcat gcaagtgatc atgccaaaca
     9781 atgatgtagc aatgttcttc caaaaattca tggtattctt tgaaattgcc ttggcattag
     9841 cattgattgc tggattattc acatggctat gcagtgcgac aacgattgta ttggtgatca
     9901 cgttctgctt atcaggcatg ttttactggg taaacatttg gttcattttt gttgctttcg
     9961 cattgatgaa cggttccgga cgtgcgattg ggttggatcg ctgggtaatt ccatggctac
    10021 aacgtacact aggtaattgg tggtacggta gaccaagttc ccagtacggc aagaaataaa
    10081 tagataaaga acgttatttg aatgcaagat ttcaatagaa aggggctgag acaaaccgtc
    10141 acagcctcct ttttatgtaa aattaaagga ataagatgcc cgagataagc ctccatgagc
    10201 aagacacagg tgtcacaacc tctgttacaa cctcttgtta taaacggtgt tccagaagta
    10261 gcttcttcga aaaataagcc gaaatttcac aaaaacttga aaagtaattt tcgtaaattc
    10321 cttcttattt cttgaagttg cccacttctg tcacaacctc ttgttataaa cggtgttcca
    10381 cctgctgctt ctcggtaata agcccaaaga attagaaaac atggaaagct gttttctatt
    10441 cttcgttctt attactcaaa gcaagacaca ggtgtcacaa cctcttgttt ttatgctaaa
    10501 attgggtgag ttaatctgga aaaaggagaa caaaaatgag ccttaaagaa tttgctgaaa
    10561 aaagtctgct caaaccttgg gatgttttta ttattttgct tttgactgtt ctttcttttt
    10621 taccagtcat gatttttggt cttcagcaaa acaccgcaac agcagaaaaa gaagcggtac
    10681 tgcgtgtgga tggcacagaa atcaaaacat tccctttgat ggctggagaa gagagcaaca
    10741 catatactta caaagatgaa cacggagatt ataacttgat cgagatagat ggcgatcgag
    10801 tacggataaa agaagcagat tgcgatgacc agatctgcgt acgtcgaggc tgggcaacga
    10861 aaaatggcga aaccatcgtc tgtttgccac ataaattagt gatcgaagtc cgttcaacag
    10921 atggaggtga cgaagacagt ctgatttatt agaccttcac acaccatgag taaattacaa
    10981 aaaatgattt atatttcgct acttgttgca caaggagtga tcattggttt attggaaaat
    11041 atgatccctt atcctttttc ttttgcgcct ggtgcaaaac taggcttagc caatctaatc
    11101 acgatcgttg ccatttttac catgaaaaaa cgagacagct tcttactact ttggctacga
    11161 ctattcctga ctactttgct aggcggaacg atttccacat tcctttatag tatgagtggt
    11221 gctttgctta gttattttgg aatgttattg gtcaagcaat taggaccaaa acgtgtcagt
    11281 atcatcggaa ttagtgcggc cggcggtttc atgcataatg tcggacagct tttgatggct
    11341 tctttcattg cacaatcatg gacagtcatg ttgtacttgc cagtcctttc agttttaggg
    11401 attttatcag ggattgcaat cgggatcgca gcgaattatc ttttacgaca tgtgcagaca
    11461 cttcaacgat tccaaaaaga ttatgagcag ggaaccaaac aaaaatggag cccaatgttt
    11521 accaaaaaat aatcgtcaca caaggagttt ttttatgaag ctacatccaa tgtgggaaaa
    11581 ctatcctgct ttaagtaaag aattgacaga aacactaaaa cggatggaag aaactgtccg
    11641 cctgaaaaac aaacctgtcg aacaagcaat caaagaaacg atccatgccg gcggaaagct
    11701 gttgcgtcct gcctatcagc tattgttctc acaatttgga ccagagcaag atcgagacaa
    11761 agccatcgct ttggcagctt ctatcgaaat gctccatacg gccacactga tccatgatga
    11821 tatcgttgat gaagcggatc tgcgacgtgg tcagcctaat atccgtagtc agtttggtaa
    11881 tgtcgtttct gtctatgcag gagactattt atttgtctgc tgttttaaac tattgtctga
    11941 ttactccact tccttgaaaa gtctccaatt gaattctcgc agtatggaaa aagtattgaa
    12001 tggcgaatta gggcagatgg atgatcgcta taattatgaa ctttctgtaa aagaatattt
    12061 ggaaaatatt tcaggtaaga cagctgaact gttccaatta agttgttctg ttggcgcttt
    12121 tgaaagtgga acaagcgagc gcttcgcaaa aaaagcagga gatatcggac tttctatcgg
    12181 gatggccttt cagattatcg atgatatttt agattataca aaagagagcc aggaaatcgg
    12241 caaacctgta ctagaagaca tgcgtcaagg ggtctattct ttaccgttga tctactcttt
    12301 gcaaaaaaac aaaccaaagc tactacctta tttagagaag aaagcggcaa tgacagaaga
    12361 agatgtcgac gccgtacgca aaatcgttga acatacagga ggcgtggaag aagctaggaa
    12421 gttagcggcc tcttacacag aaaaggcttt gaaggaaata aagaaattac ctgccacctc
    12481 tcttcgaaca aaagagaatc tcttttccct tacccaactg attttggatc gcaaagactg
    12541 aatagggaaa ggttcgatac gtaaataaat ttctcagaag gaaatctacg tgttttttga
    12601 caaattgaat tgtagttctt gtatttatga taaaaagcaa aaataaagag caacgtccgt
    12661 tagcgacgtt gctctttttt atcttgaata tttttttacc ttttcgattt cttataactc
    12721 ataaaagtga ttcttaaacc gattacaagt aatataacgc ctgcgagtgt tatccaatta
    12781 ttcgattgac ttccagtttt aggaagtgct ctttttgatt tgtttccatt gtcattgctt
    12841 gttttattag ttaagttaaa gttcgtcctg ttatccgaac ttgtttttga gaaggagaca
    12901 cttgcatgac gtgtatcgct tgtggtagac gacgtgtctg tactatcgct tgaagttgat
    12961 gaatctgagc tatcacttgt ggtagaggat gtatctgtac tgtcacttga agctgacgtg
    13021 tccgtgctat cacttgtagt ggtagatgaa tctgagctat cacttgtggt agaggtagta
    13081 tctgtactgt cacttgaagc tgacgtgtcc gtgctatcac ttgttgtggt agatgtatcc
    13141 gtactgtcgc ttgaagctga cgtgtccgtg ctatcacttg tggtagaggt agtatctgta
    13201 ctgtcacttg aagctgatgt
//
LOCUS       DANKHJ010000077        13002 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000077 DANKHJ010000000
VERSION     DANKHJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            3..371
                     /locus_tag="PW113_002383"
     CDS             3..371
                     /locus_tag="PW113_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2526662.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="PW113_002384"
     CDS             551..1492
                     /locus_tag="PW113_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2526663.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="PW113_002385"
     CDS             1561..2292
                     /locus_tag="PW113_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2526664.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="PW113_002386"
     CDS             2436..4616
                     /locus_tag="PW113_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2526665.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="PW113_002387"
     CDS             4636..5118
                     /locus_tag="PW113_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2526666.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="PW113_002388"
     CDS             5134..5895
                     /locus_tag="PW113_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2526667.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="PW113_002389"
     CDS             6026..7360
                     /locus_tag="PW113_002389"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2526668.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="PW113_002390"
     CDS             7667..8152
                     /locus_tag="PW113_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2526669.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="PW113_002391"
     CDS             8145..8828
                     /locus_tag="PW113_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2526670.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="PW113_002392"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="PW113_002392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2526671.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="PW113_002393"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="PW113_002393"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2526672.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..>13002
                     /locus_tag="PW113_002394"
     CDS             12444..>13002
                     /locus_tag="PW113_002394"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2526673.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILF"
BASE COUNT         4342 a         2297 c         2866 g         3497 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cg
//
LOCUS       DANKHJ010000078        12743 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000078 DANKHJ010000000
VERSION     DANKHJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            102..545
                     /locus_tag="PW113_002395"
     CDS             102..545
                     /locus_tag="PW113_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2526674.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(551..1735)
                     /locus_tag="PW113_002396"
     CDS             complement(551..1735)
                     /locus_tag="PW113_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2526675.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2003..2701
                     /locus_tag="PW113_002397"
     CDS             2003..2701
                     /locus_tag="PW113_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2526676.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2708..3385
                     /gene="nth"
                     /locus_tag="PW113_002398"
                     /pseudo
     CDS             2708..3385
                     /gene="nth"
                     /locus_tag="PW113_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3511..4044)
                     /locus_tag="PW113_002399"
     CDS             complement(3511..4044)
                     /locus_tag="PW113_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526677.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4341..6722)
                     /locus_tag="PW113_002400"
     CDS             complement(4341..6722)
                     /locus_tag="PW113_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2526678.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6712..7359)
                     /gene="recU"
                     /locus_tag="PW113_002401"
     CDS             complement(6712..7359)
                     /gene="recU"
                     /locus_tag="PW113_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2526679.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7423..7962
                     /locus_tag="PW113_002402"
     CDS             7423..7962
                     /locus_tag="PW113_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL2526680.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8052..8471
                     /gene="gpsB"
                     /locus_tag="PW113_002403"
     CDS             8052..8471
                     /gene="gpsB"
                     /locus_tag="PW113_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2526681.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8493..8860
                     /gene="rnpB"
                     /locus_tag="PW113_002404"
     ncRNA           8493..8860
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PW113_002404"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9069..10235
                     /locus_tag="PW113_002405"
     CDS             9069..10235
                     /locus_tag="PW113_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2526682.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10281..11777
                     /locus_tag="PW113_002406"
     CDS             10281..11777
                     /locus_tag="PW113_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2526683.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11858..12541
                     /locus_tag="PW113_002407"
     CDS             11858..12541
                     /locus_tag="PW113_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2526684.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4272 a         2230 c         2630 g         3611 t
ORIGIN      
        1 ttttttcttg gtttcagatt gggttttcct acaaaatatt tttatctttg ttgaatagga
       61 aacaaaaaaa caatataatg aaagaaaaag aaggtgtaga aatggataaa agacaactgc
      121 taatagaaga atatctggat ttagctaatg agattattca tcgtaataaa ccagaaatgg
      181 aaaaagaatt tgcaggattt actctaaatg aactagaagt gattgaacat atcgggaaaa
      241 tccctgatcc taacgtgaca aaattagcga atgctagtta catgactaga ggagccatca
      301 gtaagctaac aaaaaaactg atagctagag ggatcataaa cacttatcaa agcgaagaga
      361 ataaaaaaga aatctatttt caattgacgc caatcggaca aaaaataagg aatagacacg
      421 agcaactcca tcaaaaatgg gtacagcgag atgctactgt atttgaaaat atgtcaaaag
      481 aagaatttga cacagtattt cgttttatcg gtcgatacag agaatttata cgaagtagca
      541 aatgaagcta ctatttttta ttgtagtgat aataggcaaa aatcataagc aaaaatccga
      601 ataccgtcgg aagaaatggt gctgcagcat aaagctgtcc tgcgattagc gggccgatga
      661 cgcgcgatag agattgtata ctttgggtac cgcccattac aagaccttgc tggttatctg
      721 agacagaatt ggatagttgg ccattgaata caggagtaaa aatagaatca ccgaaaccat
      781 aacagataat cccaaataaa aacaaaatcg gcagtagaag aattccagat aatgtgatga
      841 atagataggc gaggatttca ctgaacatcc ctattcttgt gatctgctgt tctgaaaatt
      901 ttttgagcaa tcgaggcata atgaacaatt gcgagacagt atccaagatt ccaatcaaag
      961 agaagcttaa accaaccaac ccagccttcc actgaaaggc gtcaatcgaa aattgagaaa
     1021 agatagattg aagagagcca gctgccaacc aaacagcaaa acctgctgtc aataaaggaa
     1081 taacggatga aatcttgaat aattgttgta attggtgaaa aggtcttaca tgggagaaac
     1141 ttaaatctac tgaacgtttt tttattggaa ggctttctag cataaatgtg tagccataaa
     1201 cggcatttaa gaaagtaaag agtgctccaa taaatatagg aacagaattc cccaactcag
     1261 ctaatagacc ccctataata ggacccagcg ttgttcctat accgactaaa gcacccatcc
     1321 atccaaaaac tttggttctt tcattaggtt ccgtgatgtc ggcaaagtaa gcatataagg
     1381 tggagatttc tcctgccgtc aatccttcaa tgatacgacc aaggaacaac atccatatcg
     1441 aatttcctaa accaaaaagc aaatatccga tacttgatcc taataaactg ataataagga
     1501 tcggatgacg cccaaaatga tcacttaagc taccgagaat gggggcagat aagaaggcag
     1561 ctagagcata aacagacatg agcaaagtga tatagaaagc ttgctgatgt atggagtgag
     1621 aataaggcag cgttaaaaac ggaagtacag gactgacgat agtaaatcct aatccagtga
     1681 ataagacaga gacgtatcca aaaactttag aatgtttatt tgtgaaactg tacatgtaaa
     1741 aactccttta tgcaataatg ttaacttgga aacaaaaata gtgtatagta attttgtttc
     1801 cttgtcaaca ttctaaggaa cagcgaaatc tacttaattt atttattccg aaaagtgaag
     1861 atacgcatca aaaacatgaa caaacaaaaa tcaagtagac gacattgtaa atctgaccga
     1921 tcagtgaagt aaatagaaaa gaaacctgtt cttgcttccc atgttttttt tacgtatact
     1981 gtgattgagc ggaggagaaa tcatgttgaa tttagaaaat tacttaagcg ctggacaaac
     2041 agtcatatct aatcttttac ttaaaaatta tcataggatc ggtctacaat ctgaagaatt
     2101 tttatttgtt ctacagttgc atatggcaca gcttgaagga gatactttcc ctgatttgca
     2161 gatgattgct atggatatgg gaatcaaaca agatcagatt tttcaaatac tggatcgtct
     2221 cgtaacaaat ggatttatca agatagaaac gacgattgtc aacggtaaaa aagcagatcg
     2281 atataatctt taccccatct atgatctatt aggtgagtat ttaaaaacac aagaaaaaaa
     2341 atacgaagaa aaaacacaag aaaaagaaat ccagtcagtg tatcaattat ttgaacaaga
     2401 atttggacgc ccgctttctt cgatcgagtt ccagcggatc ggacaatggc tagaagaaga
     2461 ccattatcag ccggaaattt tgaagctagc actgcgggaa gccgtattaa atcaggctta
     2521 tagttttaat tacgttgacc gcattttgct atcgtgggaa agaaaaaatc tacgtacaaa
     2581 acaacaagtg gaagaagacc aaaaacgaag aaaacagcag cttcttcaaa gagaagtaga
     2641 acagtcagct caacaagaag aattgccgaa agtctcactg aaaaattggt tggaggagta
     2701 gcggaatatg ttaagcaaac aaaaaacaat ggaagctcta gaaacgatgt atggaatgtt
     2761 tcccgaagca catggagaac tcaagcacaa taatccattt gagttattga ttgcagtgat
     2821 cttaagtgcc caggcgacag atgtatcagt gaataaagca acgccagatt tgtttgcctc
     2881 ttttccgaca cccgatgcgt tagcagaagc ttcaatcgat gaaatcattc ttaaaataaa
     2941 aacgattggt ttgtatcgaa ataaagcaaa aaacatcaaa gcgtgtgcac agcagctgat
     3001 tgaacgtttc gatgggcaag tgccaacgtc aagagaagaa ttgatgtctc ttcctggtgt
     3061 aggcagaaag acagcaaatg tcgtattggg ggatgcattc gggataccag caatcgcagt
     3121 agatacccac gtggaacgtg tctcaaaacg cctgaggatc tgcaaactag atgctacggt
     3181 catggaagta gaagaaacac tgatgcggaa agtaccacaa gaattatggg taaagacaca
     3241 tcatacactg attttttttg gttgatatca ttgcacggca agaaatccca agtgcgaggt
     3301 atgtcctttg ctatcaatat gtcaagatgg gaaaaatcgg atgcgtctaa aagaaaaggc
     3361 gctaaaaaag aaaccacgcc tgtagacgtg gtttctttaa ttgcaaataa atgtatgaat
     3421 tatcttgtta ttatggagaa aagctgtttc gtttatcttt tttgcatgaa aaagtaaatg
     3481 tacatactca gtagctagga tggtaactta ttacaattta ttgcgataaa aataaaagtt
     3541 gttattttta ttgggaaaat acccgatgta gaaaataatg ccttccaata tacctataaa
     3601 ataagtccag tattccaaag gattatataa ataagtgacg tatctatcta aaggtgtaat
     3661 aaaaatgata agtgataata aaaaaatacc atttataact aaacataaac taatggattt
     3721 gggcggtttc aggctcatca gaaaaatata gattctggtg aacaacaacc ataaaaaagt
     3781 gagaatcatt gcatgagagc ttctttccat aaattttcct ttaagagtag tgcttaaaag
     3841 tacagaaaaa ataataaatg caaggatagg agcaattaaa gtaaatttat ttaataataa
     3901 acaaaatttt tcagcttttt tattcttgga ataacgaggt attgttttcg ataaaaaaag
     3961 atggtgcaga caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc
     4021 tttcaaaaaa ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt
     4081 attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa
     4141 gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta
     4201 aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt
     4261 ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg
     4321 tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta
     4381 tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca
     4441 gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct
     4501 ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga
     4561 ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca
     4621 tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga
     4681 tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta
     4741 tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt
     4801 gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac ggaaatcgaa
     4861 taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt
     4921 tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca
     4981 gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc
     5041 atataagctg tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt
     5101 ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg
     5161 aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt
     5221 tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga
     5281 ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga
     5341 acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc
     5401 cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt
     5461 ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta
     5521 gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg
     5581 atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag
     5641 gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat
     5701 agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca
     5761 ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc
     5821 ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca
     5881 gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta
     5941 tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga
     6001 ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag
     6061 tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg
     6121 tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct
     6181 tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg
     6241 atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg
     6301 gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct
     6361 accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc
     6421 ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg
     6481 gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt
     6541 cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata
     6601 aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc
     6661 acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc
     6721 attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata
     6781 aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa
     6841 aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact
     6901 attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt
     6961 ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa
     7021 agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc
     7081 attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct
     7141 cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa
     7201 cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg
     7261 attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt
     7321 cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc
     7381 acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta
     7441 atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa
     7501 atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg
     7561 gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg
     7621 gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt
     7681 ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat
     7741 tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact
     7801 cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga
     7861 aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt
     7921 cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt
     7981 tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta
     8041 gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca
     8101 aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca
     8161 aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga
     8221 gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa
     8281 cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc
     8341 ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac
     8401 aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac
     8461 gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga
     8521 tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc
     8581 gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc
     8641 tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt
     8701 ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg
     8761 gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt
     8821 tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat
     8881 taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt
     8941 caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg
     9001 aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga
     9061 gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag
     9121 aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac
     9181 gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag
     9241 cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg
     9301 aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag
     9361 ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa
     9421 aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg
     9481 aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc
     9541 tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac
     9601 cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc
     9661 cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc
     9721 ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc
     9781 aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat
     9841 tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag
     9901 aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa
     9961 cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag
    10021 aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat
    10081 ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa
    10141 ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa
    10201 ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg
    10261 gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca
    10321 atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa
    10381 aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa
    10441 aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt
    10501 cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg
    10561 taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc
    10621 aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg
    10681 aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt
    10741 tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc
    10801 ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa
    10861 cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa
    10921 tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta
    10981 tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca
    11041 aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg
    11101 atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac
    11161 aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc
    11221 ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc
    11281 gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc
    11341 ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg
    11401 ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa
    11461 caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt
    11521 ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag
    11581 aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt
    11641 cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa
    11701 cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg
    11761 tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga
    11821 tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc
    11881 tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa
    11941 aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt
    12001 gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa
    12061 cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat
    12121 ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc
    12181 cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc
    12241 aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc
    12301 tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat
    12361 caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata
    12421 tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga
    12481 atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg
    12541 atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt
    12601 tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
    12661 ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa
    12721 ttggcaggag aacaccgttt att
//
LOCUS       DANKHJ010000079        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000079 DANKHJ010000000
VERSION     DANKHJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="PW113_002408"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="PW113_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2526685.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="PW113_002409"
     CDS             complement(324..893)
                     /locus_tag="PW113_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2526686.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="PW113_002410"
     CDS             1079..1981
                     /locus_tag="PW113_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2526687.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="PW113_002411"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="PW113_002411"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2526688.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="PW113_002412"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="PW113_002412"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2526689.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="PW113_002413"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="PW113_002413"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2526690.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="PW113_002414"
     CDS             8549..8734
                     /locus_tag="PW113_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2526691.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="PW113_002415"
     CDS             complement(8796..9992)
                     /locus_tag="PW113_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2526692.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="PW113_002416"
     CDS             complement(10162..11598)
                     /locus_tag="PW113_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2526693.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="PW113_002417"
     CDS             11891..12700
                     /locus_tag="PW113_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2526694.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DANKHJ010000080        12275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000080 DANKHJ010000000
VERSION     DANKHJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            74..754
                     /locus_tag="PW113_002418"
     CDS             74..754
                     /locus_tag="PW113_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL2526695.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPD"
     gene            complement(1369..2409)
                     /locus_tag="PW113_002419"
     CDS             complement(1369..2409)
                     /locus_tag="PW113_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2526696.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2758..3084)
                     /locus_tag="PW113_002420"
     CDS             complement(2758..3084)
                     /locus_tag="PW113_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526697.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(3071..3874)
                     /locus_tag="PW113_002421"
     CDS             complement(3071..3874)
                     /locus_tag="PW113_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2526698.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(4227..4517)
                     /locus_tag="PW113_002422"
     CDS             complement(4227..4517)
                     /locus_tag="PW113_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2526699.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4653..4832)
                     /locus_tag="PW113_002423"
     CDS             complement(4653..4832)
                     /locus_tag="PW113_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526700.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4829..5179)
                     /locus_tag="PW113_002424"
     CDS             complement(4829..5179)
                     /locus_tag="PW113_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526701.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(5172..6497)
                     /locus_tag="PW113_002425"
     CDS             complement(5172..6497)
                     /locus_tag="PW113_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2526702.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6947..7561
                     /locus_tag="PW113_002426"
     CDS             6947..7561
                     /locus_tag="PW113_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2526703.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7726..7968)
                     /locus_tag="PW113_002427"
                     /pseudo
     CDS             complement(7726..7968)
                     /locus_tag="PW113_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8172..9491)
                     /locus_tag="PW113_002428"
     CDS             complement(8172..9491)
                     /locus_tag="PW113_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL2526704.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9646..10233)
                     /locus_tag="PW113_002429"
                     /pseudo
     CDS             complement(9646..10233)
                     /locus_tag="PW113_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10870..11127)
                     /locus_tag="PW113_002430"
     CDS             complement(10870..11127)
                     /locus_tag="PW113_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2526705.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(11120..11389)
                     /locus_tag="PW113_002431"
     CDS             complement(11120..11389)
                     /locus_tag="PW113_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2526706.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11520..11699)
                     /locus_tag="PW113_002432"
     CDS             complement(11520..11699)
                     /locus_tag="PW113_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526707.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         3811 a         2199 c         1900 g         4365 t
ORIGIN      
        1 gttctgttgc aaagttttaa atctactatc aaataaggta gaataataga aaaagatagc
       61 aggaggaatg acgatgaatc attttaaagg aaagcaattt cagcaggatg tgattattgt
      121 agccgtgggc tactatcttc gttataacct tagctatcgt gaagttcaag aaatcttata
      181 tgatcgtggc attaacgttt ctcatacgac gatttatcgt tgggtgcaag aatatggcaa
      241 actactctat caaatttgga aaaagaaaaa taaaaaatcc ttttattcct ggaaaatgga
      301 tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca tcgatgcaga
      361 tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag cctatgcttt
      421 tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtcacag ataaagcacc
      481 atcaattgct agtgccttta agaaactaaa agaatacgga ttttatcaag ggacagaaca
      541 tcgtaccatt aaatacctga ataatttgat tgaacaagac catcgtccag taaagagacg
      601 caataaattc tatcgaagtt tacgcactgc ctcacccacg attaaaggca tggaagccat
      661 ccgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
      721 tgaaatcaag gtattattgg gaatcccaga ttaaatcata gataccgtaa gggattttat
      781 tctttattta aaactttgca acagaaccgt ccgttgcggg taataccgta acactgaagg
      841 gcacgcatta aggcgtcatc ttaatgcttt cctgtaggac actgacggag attaccagtc
      901 tcccgagcct tacaggggcc tttctttttt tgtattttca attatcacaa tttatcgtcc
      961 aaaagtgcag aacgaaaatc aattcatcac aagcatatca gacaattaaa tttaaaacaa
     1021 ctaaaaatat acaaaaaagt gtaattgaat attagcagca ctgcctgaaa agaagtaata
     1081 tcaggctttt cagcttgggg aaataagtga attacctctc ttcatgaatt attataactc
     1141 aaaaaagcac atatgcaaac actagttccc tatttttttg aatgaaaaat aactaaaata
     1201 tctaacgatt atgactttaa aacctacgtt tgcgattgtt ttcataaact tttaatctaa
     1261 aaactccgga ataatagcta tttatttgta acatttgtaa taaaattaaa atcataaaaa
     1321 agttctaatt taaaattgat catttttata gtgatcgctt tttatcaact actctagcca
     1381 attttccgta ctaactgttg gtaattcgtt aaatgtttct ctttgttcgt ttgcaagctt
     1441 tgccgcaata gctttttcga caaaccaatt ttttacataa acgaaaatta gattctgcaa
     1501 attgtttact ttttcagttt tttgtttttg ataagctttc agcaaagtca tatataatcc
     1561 tttgtcagca tcaattccat aagattccag ttcttcataa acaacaactg tttgctctaa
     1621 ttcttcagct tttttctttg cattatgaat agtttggacg gttttttgag cgtcttcgta
     1681 agtactagaa aatgttttta tcaagtttaa tacattttct ggtacaaatg tatttatact
     1741 gtcctctttc atgatgtcga cgaagttatc tagtaaaact tgattttgta actgatcagt
     1801 ttcagtgtct attatgtgtc tattagtgtc tatagaatta gaatagaata gattgtctgc
     1861 gattttcgca attacggttg agtccttgat atgactggat tttgccgtat ttgcgatttt
     1921 cgcaattacg gtggtatcat tgatttctcg cggttttgcc gtatttgcga ttttcgcaat
     1981 tacggtggta tcagtattgt cttcctcttc catgatttta aagacgtctt gattcgttac
     2041 ttctggcttg cctaagtaca ataaattagg catggattca atcttaccgt cacgtttatt
     2101 tactcttcct ctcttctgtt tcagaagacc tgctttttct aattcctttt tgattttaga
     2161 tactttacct tcgcggcatt gtaacaattt cattaattct tcaactgtga atatgaaaaa
     2221 tatattatca tcagaatcta cccagttatt tttgactgaa taatcaaatc gatctttaag
     2281 tagcatataa gcaatttttg catcatttga tagatccatg tatttttcgt tagtaaaaaa
     2341 taccttaggc atttgataat atttttcacg ataaatttct tttgatttgt aataattaaa
     2401 atcattcata gccaatcctc tttctgtagg atagcgtttt gttcaaaact atgttacaat
     2461 gaattaaaca aatatatcta tccactatat atttttctac tgctgataag tcgccaaact
     2521 ttgttaaagc agtagtcttt ttttatcttt ttttagttta ccttaaaaaa taaattaaag
     2581 caaaccgctc tatttcaagg tttaagctca tagtaactat atcacgatat tacaataata
     2641 ttatatcacg atatcgtaat attacaatat gaagataagc gacttatcag ctttgatact
     2701 actgattttt ataaaaaaaa tatcacgata ttatgatatc gtgatattga atgtaaatta
     2761 aattttattt tgtttctttg ctagtttata tccttcaatc aagtattctt caggtatgga
     2821 ctttaatagc gcatcttcta taacgtctgg tttacttttg tctgattcag caatagtcgt
     2881 ccaatatttg agtgctgaca ttagaattgg atttatacga atgctatctg ctgttcttac
     2941 actacttcct tgatctttta tgactttact tatatttttc ttggttaatt cgatttcttc
     3001 tacttctttt gatttaaaat tctttttatt tttatttcct agtaatctac ttactgcagc
     3061 ttgactgcga ttatcttctg ccatcaaaat caccaacttc ttttaaatat tcttctgtaa
     3121 atgctttata gtcatcttca gctgttgagg gtttattaaa attgattaga tctgatccag
     3181 cagcttgcgc ttcgtcaacg gttattgtcc tacgaatata agttttgaaa atttttgcac
     3241 caatgactct agaaatttcg gttgctgcag acgtcatttc tttagaaatg ttataacgct
     3301 tattatactt agtaaataga actcctcgta cttcaagctt gtcgttcgta tattctttta
     3361 ccgtttcaat agtttcactt aactttatta gtccttttaa tgagaaatac gcagcttcaa
     3421 ctggaatgat aatttcatca gaaacagtaa atgcgttgac tgtaagcatt cctaaacttg
     3481 gtggacaatc aattataata taatcatagt tgtccttgat tggttgaagt actcttttaa
     3541 gcctgtgttc acttcctact tgagtaaatt ccctctctgc agaagaaagg gctatgtcgg
     3601 ctggcaaaac atcaattcct ttatagtcca gaatagcatc tgtaatgtca gcctcccctt
     3661 ttaatacttc ataaattgta ggttggtttg ttccatctat atcgaaacct acattatctg
     3721 tagcatttcc ctgaggatca atgtccacta aaagtacatt tttaccgtaa acaaatttca
     3781 aacatgctgc taaattaacg ctagtcgtag ttttagaaac tccacctttt tgattggcta
     3841 ctgacaatat tcttgccatt aaaatatccc ccattcatct tatattataa tataattata
     3901 tcataatatt gtgatattgc aatatcgtat atgcattata ttgtaatatt gtgtgattat
     3961 tttgttttaa agatatatat cggttcggat ataaagatta ttcatatgtt attcgaaatt
     4021 aaaataatca ctttttatta ctttaaaatt ttttattaat gataagaaat atgttttgcg
     4081 agcagcaact agaataattt aaacattact aaactgtcta tattgttact aataattccc
     4141 ttcttatagt gcattttctt agaaaaaaac aatattacga tatcatgata ttaagatatc
     4201 gtaatattat acaattgtga ttgatattat atatgttttt ttattttttt atcatgttta
     4261 atcttgaagg aatcatattc tttttctaaa gacaggagct tttcataaac atcagacatg
     4321 tttaaactag aggccaggct taaatcataa acgaatgaag caggtaatga ttttattcta
     4381 cgttgtcggg ttatccagct tgcaaccgtt ccttgtgggt aggcatataa ctcacaaaat
     4441 tgttctacag tgattcctaa attcttgata atgtaaacat tgatcgggtg cgagtaaaca
     4501 aatgtattga tagacatggt gcgtaagcct actttctttt attattgcgt ttgcaataat
     4561 agtttaacac acgcattatt ctagtatcta ctcctatttg taaaaacaaa aaaggattca
     4621 tagagataac tctatagaat ccgtaaaata ccttatatat ataacgggaa gatataaaac
     4681 ctaaaattat gataacctgc tgcagcttct acatagaaag agaagcgctc aacaccaacc
     4741 tcatttatca aacgttggcc ataaccccaa atctcttctg atagctgatc aaaggttata
     4801 tactttagct catattgctg gataatgttc attctaatcg actccattta ttatcctggt
     4861 aaaattcgat atgacgtatt tcgtcgtagt gaattttgtc ttttccaacg tagatcccca
     4921 gctcatcact acctaagatc ttgccaatga tatcttctgg atagtatcct tcagcgtttc
     4981 gttcttcttt ttgaatagat actgatctat tttttatgac tgcagcgttc aatacttccg
     5041 caatctcttc agtcgtcatt tgttcttttg ctaaattttg actggttcgt tgttcattgt
     5101 cggcatcaat ttcttcagta tgatcagata aataaaaccc tgcccacttt aaacgtccac
     5161 gatcattgta atcaatcatt tttaattccc ccatttccgc ccgcatgacc accaacaagt
     5221 gtgcttctag taatgcttct agcgccttct aacattgaag aagcatgtac aatcgacgta
     5281 aaaccatatt tttgccgaat cttgtcaata attttatcta aattttcttc gtcaatttgc
     5341 ttttgtggat cactaaataa atccagctga agcgaatcgg tgtacacaag cttgctatag
     5401 gttattccaa catgccttac ttcttgtcct tcataatact tatcaaacaa gaaaagacaa
     5461 taattctgca attcctttgt attatctgtg atagggatct tcatctggtg tgaaaaccct
     5521 ggtctcgttt ctaagatcga cgtgccaatg tgtaaatgga cacaacctgt tttacagtta
     5581 tgctgcctaa tgcgtatcgc tacttgttcc gccatttctt ttaccaccag ctcgatttca
     5641 tttcttctag tataatctcg tggtaacact tgtgaatttc catatgattt ttcctttgtc
     5701 ggttctggtg ggattgctat atctgttcga tcgatcccat tagcatggaa gtacagctgt
     5761 aagccaatca cacctagtct attcttaatc gtataaggat tccagttagc aagatctcta
     5821 atgctcataa tccccatctg attgagtctt tttttcattc gagagccaat tccccaaaag
     5881 tccgtcattt caggaatatt ccatactttt tcaggaacgt tttcgtacgt ccattccgca
     5941 acaaatcctt cattatgctt tgcttcgttg tctaaggcta gtttagccaa aaggggatta
     6001 tctccgacac ctactgtagc aatcaatcct agctcttctt taatattctc ctggatcatt
     6061 tgagccagct tctttctccg ttgacttcgt gttccttcag tcgtaaaaag attcagtgat
     6121 cgggtcactt ttaagattga ttcatcgatc gagtaaatca gtagatcttc atcagccaca
     6181 tatcttctga aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt
     6241 ggaacaacgt gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt
     6301 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
     6361 ttatcagctc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
     6421 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
     6481 tttgaataat ccatcataaa taacactcca ttctttcaca ttatacaaac atatgttcga
     6541 tattgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
     6601 gcttcttctt ttgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
     6661 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
     6721 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgagaataag
     6781 ttatcagtag acacttacaa aaaattctca tactatttcc tccatcatta gtttttgtct
     6841 atataaggtt taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt
     6901 atgattaggg tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata
     6961 catacgtgtc tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt
     7021 cggtgcagag agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt
     7081 tttccaagaa gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga
     7141 tcgacttgga agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa
     7201 tgtgaagctg atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct
     7261 ggataaattc attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg
     7321 gactgaatcg aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta
     7381 caaagggcgg cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta
     7441 caaaagcatc gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta
     7501 caatgttacg agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata
     7561 gttgatcatg ctgcttttat tatcttctca tctttaattt cctacatata attactatct
     7621 ataaaattaa gtcttactca gaacagataa agcataaaat tccaacaaac cattttttac
     7681 ttaactaaat aaagagtctg ttaatataat cttcacttat ttacgttaat cccctactgt
     7741 atttgttggt tccttatcta tattttctac aaaatcctta ttatcttgtg atttagcttc
     7801 gtataattct ttgtatgtcg acacattggt tatactaatt cctatagcta gttcttgaga
     7861 ctctactttt ctctctagct cctttgacaa agtgatttga gatttatttg gttccgttgg
     7921 agtcaattct atagattctt cataaaaatc tctatacata aagctatccc tgaataattc
     7981 atagctttaa ttctttggca cattttattg aaatatgtat cactaatatc cctatcaact
     8041 gattttgact aaaaagttac ttcaaacatt cactgttcaa aaatattgag gaataaattt
     8101 tgggtgtaag gcattttgag catacttctc cctggataac ttcccagtaa aaaaatcgtt
     8161 agattgttgg attagatcca ttgactggct cgcctcaggc gagcaaagac cctgtagaat
     8221 tcattctgat acacatgata actagataat ttgagataga ctcgtctacc cgtttggact
     8281 aatttcccag caactttcag aaacttcaat cgaatagaat gaatggtcat tcccttttgg
     8341 acttcttcaa agccaatcgt tcttaagaag ttgactaagt tgtaagctat caagctgagc
     8401 atcattcgga catgattctc caaaaagcga ggactgtctg ttttgtcaaa gtaaaagcca
     8461 gctttcgctt ctttaatgaa gttctccatt gtgccacgtt tggcatagat agaaaagatg
     8521 gtatcaggag aaacattttc tgatagattc gtcacgataa attcatgtcg aaagagtagt
     8581 tcgcccgctt cacgtgttga gcgtatacac acgcgacgac tttgtgacca ggattgtgct
     8641 tgataagtgg tcgagaagta ctgaacttct cgttcttccc acttttgatt atcgccataa
     8701 agtactgatt tctcagctat ttgacctagt ctacgattat tcttcagtcg aataacataa
     8761 tgactctttt ttgattcaca cgaatcatac acaccaggtg tagcgaaccc gctgtctcca
     8821 cgaaccaaga tgtcagtgtt tggtagagag tgattatagt gctctaataa aggtgtgaga
     8881 aactccttta cgccttttga tgtatattga tttcctgaac gtagttcagc ttttaagaag
     8941 tcaccagtca atccgtcaaa agctaccaat ggatggtaac cataggtttg gtagtgtgta
     9001 ttataattcg tttgttcttg gtgaccaaat gtatctgaat gggtcgaatc taaatcaatg
     9061 attaattccg tatcattacg gatgaggcgt gctttatcga taagttcttg gttcaaagct
     9121 tgaagttcat ggatattctc ctcagatagt cgatctaaaa atcgggaaag tgaagattga
     9181 gaagcgagtt ctttcctatc taaaacagct ttaaacacag gatcttgtct caggagatta
     9241 gctgcagaat cagctgagta accagcaatt aattgcataa tgaactgctc aagtatggat
     9301 aagttgtcat gagtaaaata cgctcgttcg tcttcaatgt gaatgtgttg cttagccagt
     9361 tcagaaaaac caaagatgtt catcagctct ttcactagga cgagacccga atcactcgat
     9421 aattgaccac ctgtatgaga aatagtgata tttgaattga attttacttg gtttttgtgt
     9481 aagctagtca tgaaaagaac tcctttcttt tggttggttt agtcacttta accatagcag
     9541 aaaggggttc ttttttcatc acttaacggg tgaagatgaa aaagtaattg taagctgccg
     9601 tgaggcagtt tcacatggtt ttaattaaaa gtatgaatta ttcaggctat cattaatttt
     9661 ataaaaaaaa ttaaatgttt ttagcactgc catataacat tcaaaacatt cttctatagt
     9721 tggtttactt ccttgaggag aatgaaccaa tttattacgt ttttctatga aatttttaat
     9781 ttcctttctt atttctttcg gaaactcaat attaaagtac cctttaaaca tgctccctaa
     9841 taatttgtct acagggggag attgtaaatt ctcaaatatt agagttaaat caggtttctc
     9901 ttgttgataa aattccttta cccctatttc acaggctgta attatcgaaa taaacctatc
     9961 tctagtattt gatatatttt tacttctttt aaggagtata agagaaaagg ggatactttc
    10021 gtgtccatgc atgtaattaa ctaattgttg agcttcagaa gttatatctc taattatata
    10081 gttaacggaa ttaggaagtg tctttaactc taatgaaatt tctatccaat tactatcttc
    10141 taatctgtat atgctcgaag tataatttga ttcgtcagca agcatcgcta catcccaaac
    10201 actgatcata aaatctttta aacgttcaat catttcagca atattgctaa aatcagctaa
    10261 tttttcttta gaataaaaaa aacatttata tcagaatcaa taaattctat ttttttatta
    10321 ctatatcgtc atcattatct tgaccattta tgtttattgt tccctgagtg gggagtactt
    10381 ctttcaaatt caagttaaat ttatattcca ttacttctcc tccaatatat aaaaattact
    10441 taactgtatc caaatttaat atgtaaataa atcattaatt tttagctaaa ttctatgaga
    10501 agtttatttt tgttgttttt gctaattaac taaaactagt acaaaaaatt taaaatagat
    10561 tattaaacct attgcttatt ctattcagtc atatatataa gcgtcatcct ttagtaacta
    10621 acatcccaca ctacaatagt attataacat atctattcaa cttcaaattg ttgaaattta
    10681 agccatttat atttactttt cctcaacttt agaaatgatt ttactaacct tatatccgta
    10741 atacgcgtaa caaacacatt cattaataac aatcaacact aagctatatt agctttttaa
    10801 atgactaggg taaataaaat aaggatcaaa aacttttaag tttctgaccc tttccttact
    10861 tccgattggt taatagtgat cttttgcaga ataaataaat atcgtttcat tttcaactct
    10921 atatatcagt ctatgttcat ctgtaattct tctggaccat tttccagata aatcatgctt
    10981 taatggctca ggttttccta atccagcaaa gggggaacga tcgatatctt ttattaactt
    11041 gttaatcttt tttatattgc ttttgtttcc ttgctcatgc caataaagat aatcatccca
    11101 agcatcatca gaccaagcct taatcatcag attcaacctc gattaagtca tgtgttttaa
    11161 atgcaccttt ggagaattgt tcatctcctc gacgaatttt ttccatgacg taattattag
    11221 aaagtgttct caacgtttct tgcatagaat cataatctct ttttgataat acaacaactg
    11281 tatcttctac atctttactt gttacaataa gtgtttcagc atcctcatta acttgtttca
    11341 tataactacg taaattttgg cggaaatttg aataagctac tgcttccatt cctttcaccc
    11401 cttatttatt tctataaaac aattgtacat tatattgtac aattaagcaa atgattaata
    11461 ttctaacttc ttataattaa aagtgctatc acttttagtt tttaggataa aaagtgacgt
    11521 cactttttga gtgctggtac ctcaaatcct gctttttctt gggcaatcaa taaatctaac
    11581 gcaatacaca taatcctgga ttcacttttg ccgtacttct gctttagttg ctgcaattct
    11641 ttttgtctct tttcatccaa cgtaacgggt aaccgtttat ttcctattgc aattggcatt
    11701 agtactctct cctttcatcg aaataaccct atgaaaaata tggctgcaat catcttgaat
    11761 agtagactca caagggctaa tacaaagcgc caaaagatca aacaaaatac cagtattgcc
    11821 aaaaatacta cgaagtttaa atgcacagct gcagcatggc gcataagtat ttcgccaaca
    11881 aataggacta acaatagagg aatgatttta ttcatatgga tctccttctt tctcttaggt
    11941 tatcgggtgt tgcccgctta tttcgcacca cctaagcggc gggggcggtt ggagcgattg
    12001 gtgcgaaata agaaccattc ccttagttac taggtttttt gtccttattt cacacattct
    12061 cacttttcgg tataaatgat tattgccttt ttttcctttc aaatcgttga tatggcattc
    12121 gtttacggtc aagttcattt tggatttctt ctgtatcgtc gtaagtatag gtgtctaaaa
    12181 aatcagttaa agtcgtgggt tctgttgcaa agttttaaat ctactatcaa ataaggtaga
    12241 ataatagaaa aagatagcag gaggaatgac gatga
//
LOCUS       DANKHJ010000081        12074 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000081 DANKHJ010000000
VERSION     DANKHJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            189..419
                     /locus_tag="PW113_002433"
     CDS             189..419
                     /locus_tag="PW113_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2526708.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="PW113_002434"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="PW113_002434"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2526709.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="PW113_002435"
     CDS             1830..2006
                     /locus_tag="PW113_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2526710.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="PW113_002436"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="PW113_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2526711.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="PW113_002437"
     CDS             2622..3089
                     /locus_tag="PW113_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526712.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="PW113_002438"
     CDS             3174..4058
                     /locus_tag="PW113_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2526713.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="PW113_002439"
     CDS             4172..4408
                     /locus_tag="PW113_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2526714.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="PW113_002440"
     tRNA            4496..4576
                     /locus_tag="PW113_002440"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="PW113_002441"
     tRNA            4583..4654
                     /locus_tag="PW113_002441"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="PW113_002442"
     CDS             4779..4949
                     /locus_tag="PW113_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526715.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="PW113_002443"
     CDS             5169..5876
                     /locus_tag="PW113_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526716.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="PW113_002444"
     CDS             complement(5935..6579)
                     /locus_tag="PW113_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2526717.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="PW113_002445"
     CDS             6705..6992
                     /locus_tag="PW113_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526718.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="PW113_002446"
     CDS             7100..7420
                     /locus_tag="PW113_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526719.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="PW113_002447"
     CDS             7501..8814
                     /locus_tag="PW113_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2526720.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="PW113_002448"
     CDS             8811..9530
                     /locus_tag="PW113_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2526721.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="PW113_002449"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="PW113_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2526722.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..>12074
                     /gene="secA"
                     /locus_tag="PW113_002450"
     CDS             10431..>12074
                     /gene="secA"
                     /locus_tag="PW113_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2526723.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKR"
BASE COUNT         4045 a         2026 c         2588 g         3415 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acga
//
LOCUS       DANKHJ010000082        12044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000082 DANKHJ010000000
VERSION     DANKHJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            73..1716
                     /locus_tag="PW113_002451"
     CDS             73..1716
                     /locus_tag="PW113_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526724.1"
                     /translation="MVPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREY
                     ENLYNSTYKESAHLRVQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIP
                     KAGGMALFFPFISVMLLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRE
                     NSEEFQETIELQQEINSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSY
                     VSLAVVILVGVHLLLTGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRI
                     RAMKETSIQEGSDSPLKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGS
                     GKTSILKLVSRLYDYDEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIR
                     IGNSQASDEEVKKAARLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLK
                     NAPILILDEITASLDAENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGK
                     LVDQGTHNELYRRSEIYKNLIKKTKLSEKFVYEKEAQSR"
     gene            2239..2508
                     /gene="rpsN"
                     /locus_tag="PW113_002452"
     CDS             2239..2508
                     /gene="rpsN"
                     /locus_tag="PW113_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2526725.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            2523..2702
                     /gene="rpmF"
                     /locus_tag="PW113_002453"
     CDS             2523..2702
                     /gene="rpmF"
                     /locus_tag="PW113_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2526726.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            2734..3111
                     /locus_tag="PW113_002454"
     CDS             2734..3111
                     /locus_tag="PW113_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526727.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            3132..3281
                     /gene="rpmG"
                     /locus_tag="PW113_002455"
     CDS             3132..3281
                     /gene="rpmG"
                     /locus_tag="PW113_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2526728.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            3305..4288
                     /locus_tag="PW113_002456"
     CDS             3305..4288
                     /locus_tag="PW113_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2526729.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            4318..4440
                     /locus_tag="PW113_002457"
     CDS             4318..4440
                     /locus_tag="PW113_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2526730.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            4458..5981
                     /locus_tag="PW113_002458"
     CDS             4458..5981
                     /locus_tag="PW113_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2526731.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            6005..6244
                     /locus_tag="PW113_002459"
     CDS             6005..6244
                     /locus_tag="PW113_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526732.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6437..6898)
                     /locus_tag="PW113_002460"
     CDS             complement(6437..6898)
                     /locus_tag="PW113_002460"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2526733.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7093..7311)
                     /locus_tag="PW113_002461"
     CDS             complement(7093..7311)
                     /locus_tag="PW113_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526734.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7429..9045
                     /locus_tag="PW113_002462"
     CDS             7429..9045
                     /locus_tag="PW113_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2526735.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEACHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(9286..10626)
                     /locus_tag="PW113_002463"
                     /pseudo
     CDS             complement(9286..10626)
                     /locus_tag="PW113_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10902..11057)
                     /locus_tag="PW113_002464"
     CDS             complement(10902..11057)
                     /locus_tag="PW113_002464"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2526736.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(11353..11685)
                     /locus_tag="PW113_002465"
     CDS             complement(11353..11685)
                     /locus_tag="PW113_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2526737.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         4023 a         1880 c         2358 g         3783 t
ORIGIN      
        1 gctttaagtg atcagggcgc agaagcttta actaaagctt ctatttctag tttttttgta
       61 tactgcatta atatggtacc agcattcatt ataatgatgc tgatagacga attagtttta
      121 gaaaatacaa agccccgttg gctttatttt gccgtatcgt ttgttacttt gcttttcatg
      181 tattggttgt tagataggga gtatgaaaac ttatataata gtacttataa agaaagtgcg
      241 catttaagag tgcaaattgc agacgatttg tcaaatttac cattatccta tttttcaaaa
      301 cataatttat cagatttatc tcaaactatc atgtctgacg ttgaaggtat tgagcatgcg
      361 atgagtcatg caatacctaa agccggcggt atggctctgt ttttcccttt tatttcagtg
      421 atgcttttgg ttggtaatgt caaaatggga ttagctgtta ttttgccaac gttatttagt
      481 tttgtcttaa tcttgttatc aaagaaatcc caaacgaaag ccaatactaa atattacgat
      541 actttgagag agaactcgga agaatttcaa gaaactatag aattgcagca agagattaat
      601 agctttaatc tatctaaaaa agttcaagac agacttttca aaaaaatgga agagagtgaa
      661 aggattcatt taaaggtaga attaagtact ttttcagtca tggccttatc ctctattttc
      721 tcatatgtta gtttagcagt agtcattcta gtaggtgttc acttactgtt aacgggtgaa
      781 gtgactatac tctacgtcgt tggttactta ctagccgcaa taaaaataaa ggattccttc
      841 gattctatga aagaagcagt gcttgaaata ttttatttag ctcctaaaat acagcgaatt
      901 agagctatga aggaaaccag tatccaagag ggatcagata gtcctctgaa atcttttgac
      961 attgagttgc gagatgtctc tttctcatat gataacaata ccccaatttt agatcatatc
     1021 tcttttacgg ctaaacaagg agaagtgact gcgttagtag gtgctagtgg ttccgggaaa
     1081 actagtatct tgaaattagt gtctagactg tatgattatg atgaaggctg tattttaatt
     1141 gatgggtatg atattaaacg tgtatctcca gcttctttgt ttagcaaaat cgctattgtt
     1201 tttcaagagg taactttgtt taatacaagt attttggaaa atatacgtat tggaaatagt
     1261 caagcgtcag atgaggaagt gaaaaaagca gctcgtttag ctaactgcga agactttatt
     1321 gaaaaactac ctgacggtta tcatacattg gtcggagaaa acggaagttc actttctgga
     1381 ggagagcgac aacgcctatc catagcacgg gcttttttga aaaatgcgcc aattttaatt
     1441 ttagatgaaa ttactgccag tttagatgcg gaaaatgaaa aacgtattca ggaaagttta
     1501 aatcgtttaa tacaagataa gacagtacta attatatcgc atcgcttgaa gtctattgaa
     1561 aaggtaaata aaattgtagt gatggatcaa ggaaaactag ttgatcaagg tactcataat
     1621 gagttgtatc gaaggtcaga aatatacaaa aatctcataa agaaaactaa actatctgag
     1681 aaattcgtct atgaaaaaga agctcaatca cgatgatgaa ataggcttta aattaaccta
     1741 caatttttgg gatatagtta taattctttt tcaaaaagaa taattttatt aaacagcatc
     1801 taattttgaa tacacgaaag aatcagtatg attgaattag ttcacttaat gtctattctt
     1861 ataatataag gaataatcaa agttttaaaa aattggcgat aaaccagtta aacataaaag
     1921 attgaataaa aagttatagt ttctttcata ttgccgtttt aagtagagga aaagctactt
     1981 aaaacggtat ttgtgtttga ttggactaat aaaagtgaat tcttattttt cagagcctaa
     2041 actgcatatt cttttaaatg atttgattat ctctatgtac cgataaattc gttttcaaat
     2101 agttatctat tcatatgagt tttatttcat gttatttatt gttgacaaga gcaactcttg
     2161 ttggtaaagt atcagtgtca aaacgtaacg attacgattt gaggtgtcta aatcaaaata
     2221 aaaggagtat aaaggtatat ggcaaaaaag tcgaaaattg ctaaagcaaa gaaacaaatg
     2281 gcgatgattg aaaagtatgc agataaacgt caggaattaa aagccgcagg agatcgcacc
     2341 gctctcgcta aattgccaag agattcaaat ccgaatcgtc tgaggcttcg tgatcagacg
     2401 gatggtcgtc caagagggta tatgcgaaag ttcggtatgt cccgtattaa gtttagagaa
     2461 cttgcccatc aagggttgat tcctggagta aaaaaagcaa gttggtaaaa ggaggaaata
     2521 tcatggcagt accagcaaga aaaacatcta aggcgaagaa acggttaaga agaactcatc
     2581 aaactgtagt aaaaccagaa atttcatttg acgaagcgaa tggtgattat agacgtagcc
     2641 atcatgtatc tttaaaaggg tactataatg gaaagaaagt aattaaagga gcttcaaaat
     2701 agtttaatat ataactttta ggagggaatc aaaatggaaa ttatttaccc acctttagtt
     2761 gaagaaggat taaagtatta tcttgaaacg acccagcaat cgctagataa atgcactttt
     2821 tatcgttcga tggtagagag aggaattatt actgaaacgg gtttaccgac ccaacaggca
     2881 attgaaaatg gattagtcaa agattactat gaggaccaag gcttatcatt tgatgatttt
     2941 ttaagaatct atccaatttt tgaagagtat gacgaggagt tatttcaatg tatcgatggt
     3001 tattgggaga tacctgttga tatgaaagaa aatttagttt cacaattgga atctggcgag
     3061 ttaacttttg aagatgcaca acaaattcaa gcatatcttg aagatcgata agtattgaag
     3121 gaggaagatt tatgcgtcaa aatattattt tggaatgtgt cgaaactgga gaacgtctgt
     3181 atctcaccag caaaaataaa cggaataacc ctgaacggtt agaattaaaa aagtattcgc
     3241 caaaattgcg cagacgggca atttttaaag aagtgaaata gctaagtggt aaaaaggtga
     3301 caaaatgggc ataccagtta caatcgtttc tgggttttta ggggctggaa aaacaacgct
     3361 aatcaatcaa gccttgcaag tctcacccta ccccagggaa gaaattatta taattgaaaa
     3421 tgagtttggt gaagttggga ttgatcacaa attattagtt catagcgagg aacgggtgtt
     3481 acaactaaat aatggatgta tgtgttgcag tcttcgcagt gatttattag caatattgat
     3541 ttctttactg gaaacaatag aagaacatca acaaccaatt tcacaaatca tcattgaaac
     3601 tacaggtata gcagatcccc aaccaatcat tcaaacactt ttaacagcgc ctcaactaag
     3661 aggaagagta tatatcgata gtttattgac tgttattgat agtgacagtt ttgaacttat
     3721 tgaaagagaa ccacaagccc ttaagcaact tgtcatggca gatcgcattt ttgtgtctag
     3781 aaaacaaaac ttgaatttat ctgcactaaa aaaattacag agagaaatca aaagtattaa
     3841 ccctttatcc gatattcgaa tgttcacaca tagtgaaacg attcaaaaaa aggattttta
     3901 taatttgaaa aaatttaatc atcccttagt ttttgaaagt gattcatcag aaaatcacta
     3961 taatggcaaa tactatcatc aacaccatca agtacatgag tttaaatcaa ttctcttaac
     4021 ttcagatcaa gacataaaaa aagatttttt actccaatgg attgactggc tgattttaac
     4081 caaccaaggg agcctgtatc gttttaaagg attgattgct ttacaagagc aggattttat
     4141 agttgccatg caagaggtaa acgaacaggt taattttcaa tacaccactc gacaaaaaga
     4201 ccaaaccact attattctaa tcggaaaaat gttggatgaa gaaaggatca aacagagttt
     4261 tgataaatta ctggcggaaa gcaaataatg ataagtagtg attgggggta atttctaatg
     4321 gagaaaacgg atatatctag tgcatatcga cgacttaaaa gcccgaatat taaaactaga
     4381 aaaagggcat taaaaattat caaagaacat aaacgaaata aaatgaagaa attagcataa
     4441 ttaaggaagg aaaaagaatg aagaaaatca gtgtaggact tattgggata gcagctctag
     4501 gattattggg agcttgctcc agcactaatg atgcaaaagt atcgaatgat aaagatggaa
     4561 aattagaaat tgtaacaaca ttctatccaa tgtatgattt caccaaaaac atcgtcggcg
     4621 atgaagcgaa cgtggattta atggttccag cgggatcaga acctcatgac tatgagccct
     4681 cagctaagga tatggcaaaa gctcatgatg ctgatgtttt tgtatatcat aacgaaaaca
     4741 tggaatcatg ggtaccaaaa gctaaagaaa gttggaagaa agctgggcct aatgtagtag
     4801 aaggaacaaa agacatgatt ttattgccag gtagtgaaga agaagatcat gatcacggag
     4861 aagaagatca ccatcatgaa ctagatcctc atacgtgggt ttcacccaaa atggcaatta
     4921 aggaagtttc aaatatcaag gatcagttag ttaaattgta tccgaaaaaa gcaaaagtat
     4981 ttgagacaaa tgctgaaaag tatttgacta aactgaaacg gttggatgct gactatacca
     5041 ctagtcttaa agaagcgaaa caaaaaagtt ttgtgacgca acacgcagcc tttggatatt
     5101 tagctttgga ttatggattg aaacaagttc ctattgctgg actaagtcct gaagaagagc
     5161 catcttctgg tcgattagct gaattaaaag agtatgttaa aaaaaataag atcaattata
     5221 tttactttga aaagaatgct aatgataaaa ttgcaaaaac tttggccaac gaagctggga
     5281 taaaattgga agtactgaat cctcttgaaa gtcttacaaa agaacaaatg gataatggtg
     5341 aagattatgt ttcagtaatg gaagacaatc taaaagcgtt agaaaaaaca actatggttg
     5401 ctggtgaaga ggtagttcca gaaaaagaag cgaaggatga aaagacagtt gctaatgggt
     5461 actttaaaga tgccgatgtg aaggatcgcg aattgagtga ttatacaggc gaatggcaat
     5521 ctgtttatcc gttgttaaaa gatggaattt tggatgaagt tttcgattac aaagcaaaaa
     5581 tcaataaaga catgacagct gccgagtaca aagattatta tactactggc tataagacag
     5641 atattgatac tatcaacatt aaagacaaca ccattgattt tgtagtcaac ggtgaacatc
     5701 atcaatacac atacaaatat gttggataca aaattttgaa ttatgaaaaa ggaaaccgtg
     5761 gtgttcgttt taattttgaa actgatgatg caggtgctgg tcgttttaaa tatattcaat
     5821 ttagtgatca tggaattgca ccaagtaagg cagagcattt ccatattttc tttggtggag
     5881 aaagtcaaga aaaactctat aatgaattgc ataattggcc tactttctat ccagcttcat
     5941 tgtctaaaca tgaaattgct caagaaatga tggctcatta aaattcaatt tttggaggaa
     6001 atacatggta agaaaagtat tattgtctat ggattccaag aatattgatg cttatcggcg
     6061 ttatcgattt gcaaagcaaa tgagattaaa tgggagtaca ttagctgata ttgctgcaac
     6121 tttacatgtt agtattgaca cagttcatag agtattaaaa cgggatcctg attgttgttt
     6181 tatcaaggtc aatactggaa gaagtagacg tttagcaaaa cggcataata gcttggccaa
     6241 ttaattgata ataccagagt agatcatttt tcccttattt ggatttgatc tgctttttat
     6301 tatgtcttga attgtcaaat caagcgcaac actttatcaa agaagacttc ataaggtgtt
     6361 ctccaatcta aacactttct aggtctgtta ttcagttttt caatcatctg atttatcgtt
     6421 gagttatcta ccgtagctat ctctttcgat tttggcaagt attctcgaat gagtctattc
     6481 gtattttaat ttgttcattg ttgccaaggg catgaggatc tggaaaataa aagggcatat
     6541 tattcaatgc ttctgtaact gcgaaatgat tggagaattc ttttcctcta tctggagtga
     6601 tcgttttgac cgcctcattt ggaatactgc ttaaaagttc aatcattttc tcgctcacat
     6661 aagcggcttt ctttctgggg accttctttc ctgctaaaag gaagcatgat tttctgtctg
     6721 tcaaagtcac taaacaagta ccaccagttt tgccagctac aatatcggct ttccaatgac
     6781 caaaatcact tctatcatct gcggattttg gacggccatg gatcgaatga gtaaggcaaa
     6841 tctttccacg agactcgaca tgattttttg tatgtcttgt ttttcctcga tggtgcaatg
     6901 aacggatcag gcctttattg ccgtgtgaga ggggttctgt atcgaaaaga tcattgcaaa
     6961 ttgcacgtta gatcgtgata aaactaattg aaaagtccat atgttcatgc ttcaaacgat
     7021 tcgagatttc ctctggcgac cactgaaaat cgacaaataa atgacgaaca attgaccaaa
     7081 ctcacggatc atttagaatg tgataacgtc cgcatttttt tcttcgataa gcataatttt
     7141 tttccgcttt ggaaggtgaa tagttttttt cttgtttctt ctcagttcat gtaaaatagt
     7201 aaacgggcta tgctgaatgt cgttgccaat tatactaata gaaaatcctt cttcgtgaag
     7261 cagaaagatt ctttctcgtt cgagtatagt aagatgacga tactggctca taattatttt
     7321 ccttctagga gtgttctcga caaacttata ctagttggat atcgattatg actcattttt
     7381 atgtgttgca cttaaagtgt aaattcaagg tatagaaaga ggttccctat gaaaaaaata
     7441 cctattacaa tcttaactgg ctatcttggt tcaggaaaaa cgatacttct aaatcatatt
     7501 ctgtacggtg atcacggaaa gaaaattgct gtcattgtaa atgacatagg ggaagtaaat
     7561 gttgatgggg tattagtcga caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg
     7621 atgccgaatg gatgcatttg ttgtacatta cgcgaagatt tgattgttga actagatcaa
     7681 ttagtgcaat tttctgactt gaatcaaatt gttattgaag cttcaggaat cagcaaacca
     7741 attccaatta ttcaagcaat tatattagct aaaaaccctt tagacattga tttgacccaa
     7801 cgattagaga ttggtgccat tattatagta gtggatgtaa aacgtttttg ggataatttc
     7861 gaaacgggtg aaaagattca ggaaagaaaa gctgaaggta atgtagttga agaagaacag
     7921 gatattcgtg gcttgttaat tgatcaattc gagtgttgta acatattact tttaaataat
     7981 agtgacttag tgtcaaccaa gacgatttac aaaattaacg gtctaattgc taagttacag
     8041 tccaatgcca aaataattag gacaattaaa ggtgaagtag acttatcgaa aattctaaat
     8101 acaaatcttt ttaatataga tgagacttcc tattcagtgg gctggttcaa agagttagag
     8161 cttggctatc aaaatcatat tcctgaaaca gaagaatatg gtatctcaag ttttatctat
     8221 cgaaatcggt tactccttga cgcacaaaaa tttgcccatc ttacggataa aaattttcct
     8281 ggcaatatta caagagcaaa agggtttctt tggtttgccc aacatagtga tttttcgacg
     8341 atctttgaaa catcgggtag ctataaaagt attgaatcat taaattattg ggtagctagt
     8401 ttacctaaaa ttgagtgtca aatgattata caaaaaaatc ccgaattgaa ggaagcctgc
     8461 catccgttgt ttggtgaacg cattaataaa ctagttttta ttggtatgaa gatggatcaa
     8521 caatgtatta aggaacaact tgagggttgt ttaactgatt ctcttaatgc agataatttc
     8581 cgaaaaattt caccatttcc atggaaaaag tctaatatgt ttagccagat atcgttaatg
     8641 aaatttttaa gaaaagctgg tgtcaagata ggcgatgtca taattattgg cattttgatt
     8701 attacctctt ttattccctt atttgtattc tctatagaaa aggagaatga aacaactgaa
     8761 gatgctatca aatatgccat cgttcgaatt gatggcaagg aaatggatcg cttcgatttg
     8821 gataagatca atcataaact cgtgaattat catcctgcga agggacaata taatattgtt
     8881 gagattaaag atggaagaat tcgagttaaa gaagataata gtcctgatca aatcgcggtt
     8941 agaactggat ggatctcaaa acccggtcaa acgagtattt gtttacccca taagttagtt
     9001 attagtattg agaaaaaaga aagcacagat tactacattt actaatctgg aaatagatga
     9061 ggaggttttt aataagaaaa agatattatt attaataata gcaggggctt tattaagtgt
     9121 gttaagcgct tgtggacaag aaaaagaagc ggtgacaaca ggcacaagta tctttaacgg
     9181 gacctgcaat ggtttagtta acagtcggtt agattactta ttttggctct tcgtcaatga
     9241 gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt
     9301 gtctagccgt gttgttgttc tggctttagt cagttgggta gtattcttag ttttcttaaa
     9361 atattttcct cgatataaaa agattcgttc tcggtagttt tgaaagtttc tatagccata
     9421 ggcaattcgt tttagtactt tgatatggtt attcaaacat tctatagggc cgttggaata
     9481 gggaacgcgc aacgctcgtt tgatttctgt ttggtatttc ttgaaggtcg tgaacactgg
     9541 ttggtagtgc tctggcaact ccttgtagtc ttcttttagg agcgcgacga attcaggaac
     9601 atcttgtctt ctgataacgg aaagaaaatc ctggtagact tcgtagcctc gctttaagga
     9661 atcatcataa gataatagcc gatcgactat ctctgcttct gtcagatgat ctcgaaatga
     9721 cggacgccag tagcgatgct gacctttcaa ttcccacgca ttcttttgaa gtagtttcca
     9781 gtatttcttt aagtgcttcg accgacgatc ccctttttta aaggtcttca ttacttgaat
     9841 tcggtggttg gtaaaagccc gaccaatgtg ttggctaata tgaaagcgat cggcgacgat
     9901 tcgggcattc ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac
     9961 cactagacgc acttgttcac ggacttttat agggtagcgc aagaaatagt cgcgtaaagg
    10021 attgagttgc cgattttcca caatatcaat cagttgatgg gtcttgccgt ccatcatgac
    10081 aaagctcatg gctcccgaga catttttcac tgatttgaat tcatcgaaac acaccacttc
    10141 tggtaacagc tggctcaaag aaatggtttt aggccgaaga ctgcgaagta ttcgaatgac
    10201 agtggtcgga gaaatgtgct tcatacgtgc gatcaaagac atagaaagag gttcgcttaa
    10261 tagctcaaga atggcttgtt tgactctgcg agcaatggag cagcgacgat cactaactga
    10321 atcttctgct aaaaaagtct ttccacagtg tttacatttg aagcgctgtt tgttaatccg
    10381 cagaatggtc ttgtagttac tgacatcatt caataagatg gatacttttt tccagcccta
    10441 tttaatgatc gtttgttcat tttgatagtg gcgatgaaag cattcctccg gttgataggt
    10501 caatgtgcca tcaaaaacaa aacatgtttc gtcattgatt cgctctttgc gaaaggcatc
    10561 ttcatgaaag ataagattta ggtctagaat atctagtaac tctttggtaa actctgaata
    10621 ggacatcttg aaacactcct ttgaatggtt ttcgtcgact ttattctaaa ggattttaag
    10681 gtgtctgttt gcgtttatta gaaaaaagaa aaagtattgg caacagaagg cgtcaccaac
    10741 actagatatt atagatccct tattttcttc gcttcaaatt gtcaaggagt catcataaat
    10801 tttgtatgga ttcttttgtt tgggtagtag tcttctaaat aatcttggat taacaccttc
    10861 atctctattt tacttttata tttgttggag tcgattaatt ctcatttgat tgatcgatga
    10921 aacgcttcaa ttactgcatt gtcaacagga gtcccagttt ggctcactga atgtgtgatg
    10981 tggtggtcgt tcaacagctc ttgataaata aaagaccgat gctggctacc tctatcggaa
    11041 tgaattaccg taggcaattc tccttaataa atttgtaatg gacggacgac aatctctttt
    11101 ttcatgtgtg aatctaccat atagtaacgt agaatacgag ttgctaagtc aatataggtg
    11161 gacaaataga ggcgaccgtc attacatgga atgtaagtaa tatccatcac ccaaacagca
    11221 tcaatttggt tctgttcaaa ttatcgattc aacatttttt taccatttct ttgattgttt
    11281 tttctctacc atgagaagat ttgcgtcgat gaaatcctct tgtgcataaa ttcatttcac
    11341 gcataagaac agctacaaca cgtttattga tttcgattcc ttcatcgtaa agtttttgag
    11401 tgatccctgg agatccgtat cgatctttgt gttcgtagaa cactcgtctt cctcttttag
    11461 tcgtttattt tcctgttcaa gaatgtagaa atctgcttta tcaggtaatc tttttccttg
    11521 tccagcaaaa gcatttttcc cgtatcgacg atattctgaa acccatcggg aaattgttgg
    11581 acgagagata ttgtattctt cagcaatttt cttggtagtt acttttttct caaggatatc
    11641 ctgtgaaatc tttagtttaa atttacgatc atatgttttt ctcaatagag ttcattcctt
    11701 ccggtaacgt aactgatagt caagaaaagt gtatcatagc ccaacttagt atcagggttt
    11761 gtcaactaaa ctgtggaagt tgatagataa gagtttttaa caaccaaaat tctcttgact
    11821 atttttgaaa tcagacaaat tttgattgga cacatagttg aataaatcta ccgtcgttac
    11881 ggaaggtaaa tcgcatgctt ttcaattcta gaggagaagt cctcgttgaa gtaccatagc
    11941 ccagttctgg attcgacctc ctgcccactt catacgtaaa taaagtaatt ttaggagtga
    12001 gactgtgaca aaagttattt ttaagctaaa aacgaattaa aaaa
//
LOCUS       DANKHJ010000083        11784 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000083 DANKHJ010000000
VERSION     DANKHJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            510..1073
                     /locus_tag="PW113_002466"
     CDS             510..1073
                     /locus_tag="PW113_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526738.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1134..1499)
                     /locus_tag="PW113_002467"
     CDS             complement(1134..1499)
                     /locus_tag="PW113_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526739.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1575..1820)
                     /locus_tag="PW113_002468"
     CDS             complement(1575..1820)
                     /locus_tag="PW113_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526740.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1890..2144)
                     /locus_tag="PW113_002469"
     CDS             complement(1890..2144)
                     /locus_tag="PW113_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526741.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2308..2631)
                     /locus_tag="PW113_002470"
     CDS             complement(2308..2631)
                     /locus_tag="PW113_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526742.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2818..2891
                     /locus_tag="PW113_002471"
     tRNA            2818..2891
                     /locus_tag="PW113_002471"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Ile,seq:cat)
     gene            complement(3276..3773)
                     /locus_tag="PW113_002472"
     CDS             complement(3276..3773)
                     /locus_tag="PW113_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2526743.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3813..4622)
                     /locus_tag="PW113_002473"
     CDS             complement(3813..4622)
                     /locus_tag="PW113_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2526744.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4956..5813
                     /locus_tag="PW113_002474"
     CDS             4956..5813
                     /locus_tag="PW113_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2526745.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5806..6708
                     /locus_tag="PW113_002475"
     CDS             5806..6708
                     /locus_tag="PW113_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2526746.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6741..7091
                     /locus_tag="PW113_002476"
     CDS             6741..7091
                     /locus_tag="PW113_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2526747.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7140..8063
                     /locus_tag="PW113_002477"
     CDS             7140..8063
                     /locus_tag="PW113_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2526748.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8141..9034
                     /locus_tag="PW113_002478"
     CDS             8141..9034
                     /locus_tag="PW113_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2526749.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9072..9635
                     /gene="amaP"
                     /locus_tag="PW113_002479"
     CDS             9072..9635
                     /gene="amaP"
                     /locus_tag="PW113_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2526750.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9649..9837
                     /locus_tag="PW113_002480"
     CDS             9649..9837
                     /locus_tag="PW113_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2526751.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9850..10752
                     /locus_tag="PW113_002481"
     CDS             9850..10752
                     /locus_tag="PW113_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2526752.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10784..11026
                     /locus_tag="PW113_002482"
     CDS             10784..11026
                     /locus_tag="PW113_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2526753.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11282..11653)
                     /locus_tag="PW113_002483"
     CDS             complement(11282..11653)
                     /locus_tag="PW113_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526754.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4176 a         1985 c         2233 g         3390 t
ORIGIN      
        1 attcattggt cgttgaatgg gctgaatagt agtgtataaa acaattttgt ggagcgtgaa
       61 gcttttatgg tagaggaaaa tacgaataaa gtgatgttga taaaaagatt cctatcttga
      121 atatgaatcg agatgtcttg aaaatgaatg agtttgacca atcattatcc tatcgaaatt
      181 tacagtttcg ttcgattggt ctgtcaatta atgaaaaagt aaaaaagaat atgttctagg
      241 aagagggcgg cacatctaaa tttttaagta acggacagtt caaatatcaa gcattgaaag
      301 aggctatcct actgtttgaa ggaatacgaa tatataaaaa cgacaaagag gaaaaagtag
      361 ttcattaaat cccttcaagt gatgaaagaa tgagatctaa taaaacctga tctttcatct
      421 gtttttttaa taacgtaata atttttttat tttttgtgct ttcgccgtta ttgtagagtg
      481 atttagcatt cttattttta aaaataactg tgttatatat taaaaaaggc ataatagtta
      541 taagcttttt taaagaatat ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa
      601 gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
      661 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
      721 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
      781 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
      841 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
      901 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
      961 ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
     1021 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
     1081 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
     1141 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
     1201 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
     1261 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
     1321 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
     1381 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
     1441 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
     1501 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
     1561 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
     1621 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
     1681 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
     1741 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
     1801 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
     1861 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
     1921 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
     1981 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
     2041 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
     2101 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
     2161 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
     2221 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
     2281 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
     2341 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
     2401 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
     2461 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
     2521 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
     2581 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
     2641 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
     2701 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
     2761 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
     2821 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
     2881 tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2941 gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat gcaagctttt
     3001 gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagcttaaca
     3061 aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag ggtcagctga
     3121 ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt cataaaaaag
     3181 ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg ctttttttat
     3241 tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat agagacgttg
     3301 aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac taacattgga
     3361 tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt agagagtaca
     3421 cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt gctttcaaat
     3481 aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata ctttttaatc
     3541 actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc tcgttcttct
     3601 tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc attattttgg
     3661 cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc tggaatataa
     3721 aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa cattttcttt
     3781 cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa aatataagca
     3841 acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac ttgtcgctgt
     3901 gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc gttttcttga
     3961 tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag aaaaacaagt
     4021 gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc agagaatact
     4081 gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc ttcactctta
     4141 tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt taaaatttga
     4201 tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt gactagattt
     4261 gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat ggatattttc
     4321 gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt tgaacgtctg
     4381 agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt ggaagaaatc
     4441 aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct gttccttccg
     4501 gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg gatattttct
     4561 tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc
     4621 attttatctt cctactttct ttttacatat ctattttcct gttaccttaa gattaaatga
     4681 gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa aaaacattct
     4741 taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata aaaagccatg
     4801 aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt aaaatattca
     4861 atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta gatgtagaac
     4921 aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac ggcgtagttc
     4981 ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg atcgcaactg
     5041 cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac gatggtctat
     5101 cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt aatgcaagta
     5161 tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt gaccatatca
     5221 caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag gattataaga
     5281 aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa ctatatgatg
     5341 agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg aaagctttgg
     5401 aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg gtgattgaca
     5461 ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc agtgaaaatg
     5521 gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat gaagattggg
     5581 aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt catccagcac
     5641 caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg atcgttcact
     5701 ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc aatttatact
     5761 taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa taaaaaagaa
     5821 atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc attatttgtc
     5881 tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat tgacgatgat
     5941 atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg aatgaactca
     6001 gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat cagcgatcgt
     6061 attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg cacagaagtt
     6121 gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc taaattatgg
     6181 cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt ccatcaacga
     6241 ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg tttgatgctg
     6301 atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga tttcatcgaa
     6361 cataatccag aaaaattacg attccatgac caagatgcat taaatgctat cttacatgac
     6421 cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc aaaagcaaaa
     6481 aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa tccttacatt
     6541 atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc aacgaagaaa
     6601 tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt ccctaaatat
     6661 ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata gaagaccaca
     6721 actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta gagacgttaa
     6781 ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat tccgatccat
     6841 ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt ccttttgttt
     6901 cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc tatacatcta
     6961 aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa cagcttgaca
     7021 gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta aagaactgct
     7081 tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga tactactttg
     7141 tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg ctcgtacttg
     7201 atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt cctgatgata
     7261 ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt tatgagacga
     7321 aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat aatcgtatcg
     7381 aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt acttgtatcg
     7441 atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat ttaaactcgt
     7501 ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg aaagaactaa
     7561 gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca acggaaaatg
     7621 aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat cataccttaa
     7681 gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc aaacgtttag
     7741 acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa aaaatgatcg
     7801 agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg atggacaata
     7861 atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc gttccggcgt
     7921 tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt tctgctctag
     7981 gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt taccatttat
     8041 accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac aaagaaattt
     8101 gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac caaaattaat
     8161 cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg atacaccagc
     8221 tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg aaggcaataa
     8281 tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg gtcgtgcagc
     8341 agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc caggagaaga
     8401 aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga ttgtcttact
     8461 accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag ccgtgagtga
     8521 acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt gtcagtctat
     8581 tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca tcgctatgtt
     8641 tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg taacaacaac
     8701 ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa caaaagcttc
     8761 aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa tccgtgtaaa
     8821 tggtgtagca cctggtccaa tttggacacc attacaacta gacgaaggac aaccttctga
     8881 ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc caggagaatt
     8941 agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag cccaaattta
     9001 cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca cccctatgag
     9061 gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca ttacttttgc
     9121 tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct tttcagctag
     9181 agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta ttttggcttg
     9241 ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt tatccaaaaa
     9301 caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat aaaaaagcaa
     9361 ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca ccaaaagtaa
     9421 atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg aaacgaactt
     9481 cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa gtacaacaca
     9541 tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt gaagaaccag
     9601 ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat gaaagaacta
     9661 ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt agccgtttta
     9721 tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc gatcggcgtc
     9781 gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa atcttaggag
     9841 gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa ccaaaaacca
     9901 gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa cgatgaaaag
     9961 aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt tcaacaagga
    10021 gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg aactccagaa
    10081 tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga agagcaaaaa
    10141 catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac aaacggacaa
    10201 ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga acaggaagat
    10261 tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat cggaatcgca
    10321 attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa tgtggcagga
    10381 aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg taaaaaacaa
    10441 gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa aatctttgaa
    10501 gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga agtgatcgaa
    10561 gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga tcaagaaacg
    10621 gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc gaatcaaaca
    10681 gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa tacagaacca
    10741 cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat ttatttggtc
    10801 acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata aaggttcttc
    10861 gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg gtcaagcatt
    10921 attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat tgattggtgc
    10981 catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag acttttaaaa
    11041 agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca aaaatagctt
    11101 ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga acactgttta
    11161 tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg cgggcaaatt
    11221 cccaattaga tttttaaaag tttttcctta aatgtgtctt tattgataca gagaagcttc
    11281 tttaataaaa caatgcctcc ctaaattttc ttgagatagg aaaggtgaac atacaaaata
    11341 atacgaccac tttgaaaacc cagatgatcc acaaatggat cgtatgatac tgatgaagta
    11401 aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa taatatctct
    11461 tttttactct tgtctgtttt agccaataat agctcgtaaa gtaatctaga caaacaaata
    11521 gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg ttgctctgct agacgattga
    11581 cgagcaggaa aaatgttggt aatggaatat agatcacgag taatgaaaga aaaaagtaaa
    11641 caataaatgg catactattc ctcctaattg tgtgagttcg ccctcatttt aaaataacga
    11701 tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat ttttaaataa
    11761 ataatcatta ttatcaaaat aatg
//
LOCUS       DANKHJ010000084        11735 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000084 DANKHJ010000000
VERSION     DANKHJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            558..1163
                     /locus_tag="PW113_002484"
     CDS             558..1163
                     /locus_tag="PW113_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2526755.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1179..1751
                     /locus_tag="PW113_002485"
     CDS             1179..1751
                     /locus_tag="PW113_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2526756.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2003..2260)
                     /locus_tag="PW113_002486"
     CDS             complement(2003..2260)
                     /locus_tag="PW113_002486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526757.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2386..3201)
                     /locus_tag="PW113_002487"
     CDS             complement(2386..3201)
                     /locus_tag="PW113_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526758.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3718..5043
                     /locus_tag="PW113_002488"
     CDS             3718..5043
                     /locus_tag="PW113_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2526759.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5036..5386
                     /locus_tag="PW113_002489"
     CDS             5036..5386
                     /locus_tag="PW113_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526760.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5368..5553
                     /locus_tag="PW113_002490"
     CDS             5368..5553
                     /locus_tag="PW113_002490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526761.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6457..6732)
                     /locus_tag="PW113_002491"
     CDS             complement(6457..6732)
                     /locus_tag="PW113_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2526762.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6704..7657)
                     /locus_tag="PW113_002492"
     CDS             complement(6704..7657)
                     /locus_tag="PW113_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2526763.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8269..9762
                     /locus_tag="PW113_002493"
     CDS             8269..9762
                     /locus_tag="PW113_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2526764.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9897..10193
                     /locus_tag="PW113_002494"
     CDS             9897..10193
                     /locus_tag="PW113_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2526765.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10294..11145
                     /locus_tag="PW113_002495"
     CDS             10294..11145
                     /locus_tag="PW113_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526766.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11166..11449
                     /locus_tag="PW113_002496"
                     /pseudo
     CDS             11166..11449
                     /locus_tag="PW113_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4036 a         1809 c         2175 g         3715 t
ORIGIN      
        1 tgcttatagt gttggttctt tgataaaggt aaacgtgtca cttgatctat ttactatttt
       61 actagcggtt ggggtatctt cggttattgg actggtcttt tctgtaatgc ccgcttcaga
      121 ggctgcaaaa aaagatttaa ttgatattct tagataaaat cagaatgaca agaatattta
      181 cgttctagat tctaaaataa aacacaaaga gaccaaccag gcaccctatt gtttctttgt
      241 agtatagaga tattctttgg cttgtaagag atcaatagag ttaggtgacc ggagttcgaa
      301 ataaaggaaa tttttgattg tataggagaa ttctttatcc ctaatattta gaattttgag
      361 tatgccatgt tgatgtttta gattctctat aaatatagag gtgtatcaaa ggtcaactag
      421 actgtgcaaa atagagacac ttgaaaaatc tatcgggggt cagaaactta atagtttttg
      481 acacataaac aaaatcgcga tataatgggg ttgagagtgt ttcataaact tatgtcataa
      541 agttaaggag atttcgcatg attttagaga ataaattggg attaaccaat caagttgagt
      601 tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa agaattgtac gattgtggaa
      661 aaataaatga aatcgaagtt ggtacattta aaggtttatc tgaaattcat gaatttctat
      721 tttcggatat ttacgatttt gccggaaaaa tccgatcggt caatattgca aaaggaaatt
      781 ttcgatttgc accagttatg tatttagaac attcgttaca gcatattgat cagatgccac
      841 aaacttcttt tgaagagatt atcaagaaat atgtggagat gaatatcgct cacccatttc
      901 gagaaggaaa tggccgaagt actagaattt ggctggatct cattttaaag gaagaattgc
      961 aaaaagttgt agattggaac ttaatcgata aagcagacta tctttctgca atggagcgaa
     1021 gtcccataaa cgatttggaa atacgctatc taatttccaa tgctctgact gataaaattg
     1081 acgatcgcga gttatatatg aaaggaatcg atgtcagcta tttctatgaa gggtattctg
     1141 agtatacgat agatgatttg taaaagtagg tggaccaaat gacgaaatat ggatatgcac
     1201 gtgtctcaac cgcttctcaa agtacaaaag aacaaattca acagttgctt gagaacggcg
     1261 tagagaagaa gaatattttt tcagaaaaat ttacgggtac aaaacgggat cggccaaaat
     1321 tcaatcagct gttggctggt ctgaagcctg gagatgaaat agttgttacg aagcttgatc
     1381 gatttgctcg taacacgaga gaagcattag agatcattga acctttactc gatgataacg
     1441 taacgatcaa ggttttgaat ctaggaacga ttgaaaatac gtccatgggg cgaatggtga
     1501 cacggacgtt gttgagtgta gcagaaatgg aacgtgatat gattgtggaa cgtacccaag
     1561 aaggaaaaat gtttgcaaga aagaataatc ctaatttcag agaaggacgt ccgaaagcaa
     1621 caatcacacc gaagaagagg cacgcatatg aactgcttac ttcaggtaaa tcgtataagg
     1681 aagttgaagc tataaccggt ttcagtcgca gtacgctttt tagaataaag aagaaaattg
     1741 aagaaagtta aaaaagaagt ctcagcgaac cagacaggta aatacttaat gcgttctaga
     1801 tatgagtcta ttagcatgtt gtgaaatata atattttttt atttacttga tttctgctca
     1861 aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa ggtatgctag ccacgcttac
     1921 tatattagga taccatcata tatttataat gaacgatttg ttcgtctacc aaacaacaca
     1981 tatcgttcag cgtaagtcac gtttaggaac gtggcttttt tagtgttatt atcgataaat
     2041 aatctattga ctctattgat tttatctcca tatccctacg gtgttgattg tctttagatc
     2101 tttcatttaa gaaccaaata actatcataa aaatcagcac tgctgctaat ataataaaca
     2161 gcattcctat tgtgatatta cctattgatt ttaccataac tacgattaaa gtaataatgg
     2221 cattcaagtc tcctttgttc tctatatata aattagccat aaatcatctt cctttcaaat
     2281 taagatactt gtttgatacc aataaatagt aagcgtggct agcatacctt tttctgtaat
     2341 agcaagtgtt ctaatagtcc aaacttaatg atggaccgat gctagttata gtacagtaat
     2401 gttttctttc ttatctagat ttttagtaag ctctatcagc aaatctaaca aatctgagtc
     2461 cttttcagaa actaaattaa atctagtgtt tacaacaaag ttttcatgtt ctatattgtt
     2521 tagattattt gcggaaagga gggcttctag atcttgacat tcaaatctat atgcatcgtc
     2581 ataaatctta ctagaaggag tgcttaattc gaaattactt attttctcat cttggaccaa
     2641 gataaattta tagatagtat ctttttgtgt ttttctttta aaaacacaac agagtgtgtt
     2701 ataaggaacg ttataatttt catcagcctc attttttctc cattcaaaca aatgataaac
     2761 tgcaaccaag ttgccatcaa tttcatggac agtgctctcg gtaacatcct ttaaagaact
     2821 atcaagggct aatttatagt aactagagag aggaagtaaa attgtcgatt tactgagatg
     2881 gttatcgtct atacgtgcaa tcatacctaa atcaactaag gttttattcc aggtgtttat
     2941 agttttagtt gtcactccta gagactctgc tatggtattg accgaatgcc aggaatcccc
     3001 gccttcattc tttgccttaa aacagtaata cagatataaa ttaatagctt tgttattcgg
     3061 taaatatttt tcaagttctg ttggtagaat aaagtaagaa tccttaatgt tttttctgaa
     3121 ctgtctccag tctctgtagt tttgtttata aagttgtgaa acggtttttc cacctttaga
     3181 aagtcctgct atcttaacca atcttattgc tcctttcttg aatctgtcat gatcatacag
     3241 tattttttac ttgtagtcca gcaccttaat atattatcct atgtaattaa tttcgtacta
     3301 gatagtaatt attttttatt taattctcta gtcacttaca aataagttat ataaactacg
     3361 attaataaat tattacaaaa actattaaga aaacttctac atacaactat tacaaaacta
     3421 ttaagaaact gaatactcct attttttgtt tttaaggaaa gttttttctt ttttgaattt
     3481 cttatttttt tctaatataa ctaagcaaaa ctggataata cgacacttat tttcaaattt
     3541 tcacaagact aatagtaaat atccatttaa taaagtgata caatgtgttg attttacaaa
     3601 tcaacagaag aagtagggaa ataacttacc tatgcttctt tttttgtaca tttaatcgat
     3661 tcttctttac aaaaacgaac gtatgtttgt ataatgtgga agaatggagt gttatttatg
     3721 atggattatt caaaagagcc agtaaatgat tactttttga tcgatatgaa aagcttttac
     3781 gcaagtgtcg aatgtataga aaggaattta gatccattaa cgactgaatt agtcgtcatg
     3841 agtcgagcgg ataatacagg atcagggttg attttagctt cttcaccagc tgcaaagaag
     3901 aagtacggta tttctaatgt cagtcgacca cgagatctac cgcaaccttt tcctaaaacg
     3961 ttacatgtcg ttccaccacg catgaatctc tatattaagc gaaatatgca ggtaaacaat
     4021 attttcagaa ggtatgtagc agacgaagat ttattgattt actcgatcga tgaatcgatc
     4081 ttaaaagtga ccagatcaat gaatcttttt acgactgaag gaacaagaag tcaacggaga
     4141 aagaagctgg ctcaaatgat ccaggagaat attaaagaag agctaggatt gcttgctaca
     4201 gtaggtgtcg gagataatcc cttattagct aaacttgctt tagacaatga agcaaaacat
     4261 aatgatggat tccttgctga atggacgtat caaaatgttc ctgataaagt atggaatatt
     4321 cctgaaatga cagacctttg gggtattgga tctcgaatga aaaaaagact caaccaaatt
     4381 ggtatcatga gtattaaaga cttagctaat tggaatccat acactattaa gagccattta
     4441 ggtgtgattg gcttacagct gtattttcat gcgaacggga tcgatcgaac agatatagcg
     4501 atcccaccag aaccgacaaa ggaaaaatca tatggtaatt cacaagtgtt accacgagat
     4561 tataccagga gaaatgaaat cgaactggtg gtaaaagaaa tggcggaaca agtagctatt
     4621 cgtatcaggc aacataactg taaaacaggt tgtgtccatt tatacattgg cacgtcgatc
     4681 ttagaaacga gaccaggttt ttcacaccag atgaagatcc ctataacaga taatacaaag
     4741 gaattgcaga attactgtct tttcttgttt gataagtatt atgaaggaca agaagtaagg
     4801 cacgttggaa taacctatag caagcttgtg tacactgatt cgcttcagct ggatttattt
     4861 agtgatccac aaaagcaaat tgacgaagaa aatttagata aaattattga caagattcgg
     4921 caaaaatatg gttttacgtc gattgtacat gcttcttcta tgttagaagg cgctcgtagt
     4981 attactagaa gtacattggt tggtggccat gcaggcggcg caggagggct taaaaatgat
     5041 tgattataat gatcgtggac gtttaaagtg ggcaggtttt tatttatctg atcatactga
     5101 aaaaattgat gccgacaatg aacaacgagc caatcaaaat ttggcaaaag aacaaatgac
     5161 gactgaagag gttgcggaag tattgaacgc tgcagtcata aaaaatagat cagtatgtat
     5221 tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa gatattgtta gcaagatctt
     5281 aggtagtgat gagctgggga tctacgttgg aaaaaacaaa gttcactacg acgaaatacg
     5341 tcatatcgaa ttttaccagg ataataaatg gggtcgatta gaatgaacat tatccagcaa
     5401 tatgagctaa agtatataac ctttgatcag ctatcagaag agatttgggg ttatggccag
     5461 cgtttgataa atgaggttgg tgttgaacgc ttctctttct atgtagaagc tgcagcagga
     5521 taccataaat acgaatacta tattggctat taaagtgcaa taaaattata tttcttttat
     5581 gttttacatt ttgtatcaat tctgataaaa ttacctatag gtaattaaaa aatataaaaa
     5641 aactagttac aaccttagga cgttgtaact agcttgaagc atactagagc taactaaaat
     5701 agcactagaa agcaatgtta aacccgtcca acaaacttga tttaacattg agaccagctt
     5761 attcagctaa atatagttga ataagcttct tttgttttaa aaaaacaaaa ggtgtactac
     5821 gaataacgca agtaaaagaa atactattac aaccagtagg ctacaagtaa gtattactaa
     5881 acattgcgta gttagaacct ttgctctatt gtctaaagcc acaaaattag agatcagctt
     5941 tatgatgcta agagcgagct gcatccatgc ggacataggt tcttttgaca ctattaattc
     6001 tcctttttag gataagggaa attgctagta gttttttcat tagcaccatc ccccttcatt
     6061 tgattgagtc tagtgctgct gtgatagttt gctgtttaat atgcttacat tcttattata
     6121 acataaatga aacaaacaca tggttagagg tcgtatcaat caaaatagtg tctcacaaac
     6181 gatttattac ttactcctgc tgactaacgg actgttcttc agtaactgta actatatgtt
     6241 tgttaaaacc accattacct gtagataaaa accaatatat ttactatact cactatatta
     6301 aatatattta gtatagtaaa aactagaaac aataaaattt gtgaatgttg tataccccca
     6361 aacaatacac atattttgaa ggattaggat taataaagct aaaaatatag taagtatact
     6421 aaatatattt actatatcta gtatacttac tatattttaa tcttttgatc gtctattata
     6481 tataatccgt tcctcgtcgt ttaaagtatc aacataggcg tccattgcaa gtttgactat
     6541 ttcatacata ggaatatctt ttgcataact aagatcccga atggttttta gtacgggagg
     6601 atctacttga actggttttc tttctttcgc agtgtacttt cgttccccta actcgacatt
     6661 aatatcatct ttaattgaaa tatcttcagc accaattttc ttcttatctt tgttcgttgg
     6721 taataatgcc atttagttta atctcctttc caagaggtag gatatgattg gccattgagt
     6781 cagcatattt aggtgtataa tcaaatcctt caatatcacc atgttgttca tagtattcaa
     6841 ttctttcttc taattctgta aagatgtctg caaaaatagc ccaccatctt cgatctacat
     6901 aatcatttaa ttggatacca ttctcaccat acatttctaa ccgatccgag cctttaacaa
     6961 tgcttttaaa aatgtttgat tcaccatact tatcaattgt tttttgatag tttgcgtgtt
     7021 gagaggaacg tcgcactgag aaaagaacag gcaaaattcc aatgatttga agatgagact
     7081 cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat aaaatcttct gttccttcca
     7141 ttgcgtaccg ttttaattct tgcattgcaa taatataatc tgcagcagtt aagaatgcac
     7201 gaaccacatt atctgttgat ggtgggcagt cgataaagat ataatcgaaa ttttttctca
     7261 attcttctaa aggtttaatg aaggccaatg atctcttacg acgatcagga tatttttcgg
     7321 acaagtattc attaagttct tcagatccag gagagccagc cataatatat aaatgtggac
     7381 ttacttgagt aatacctgaa acccagtccc cttttttgat tgctgcagac atggatcgat
     7441 caaagctagt aatttgatac gttgatccaa tgttggaagt agtatttctt tgttggtcat
     7501 aatcaattag caacacgtct ttttcaaaaa gttgactaga aacaatcgct aataaatcat
     7561 tgtctgtagt cttaccaatg ccgccttttc tacctgtaag cattacaact tttcctagtt
     7621 tatctgatgt tttaaatgca gtccgtaagt tttccatagt tccctccaat ttcctatact
     7681 aaatatattt agtatacttc atatacttaa tatattcgat atatctaata tactccaaat
     7741 tgataagaat tcagacttta taaggaatat agtggatata ctaaatatat ttgctatatt
     7801 cagtatatca gatatattca aaatgtcaaa atatatttgt gcgttttgcg ttgacaattt
     7861 agagtgaaaa gaggtaaact taattctata gaaaccacag ataattcact atttacaaag
     7921 tagcaaaagc agtggtactc tttaaacaga aaaattacat aaaaaaaaga gacggaaata
     7981 attgatttta gcagaatcaa ttatttctga atcacatggt taggacatgt gttcacgctc
     8041 gtctcaagaa tatgtaagta gctaccaact acttatatta aaagttgatc ggataccttt
     8101 tccgattgcc tttattctac caaaaaaagg cgagcaactc aacagaagaa cgtttattta
     8161 gtgtacttaa attaaatatt cttaaacact gaagagagaa cacatactat ggctgagatt
     8221 aaagctaaac tgtgttctct ttttgtttga gaaaggacgt tacaatcgat gttgagcgaa
     8281 atttattcag ctattcttaa aaacggctta cgtacataca aatatcgaaa cagcaccatg
     8341 aaacctgcag gtcatattga ttccagtagg agtggtgcta tctttgcgta ccgtagcaag
     8401 caattaatga atgttgggcg tggtatggtc attacttcgg aagaagctat tttagagaac
     8461 gaaaagaaac taacgcattg gacacctaat acctatcgct ttggtactta tgcggatgat
     8521 tatcgaacag tggttaaagg tcattctgag aaaaacttaa gtcaaatcaa cacatttgta
     8581 gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta ttttagcctg tttagatcaa
     8641 gtgggctaca tgccaacgct aattttagaa tcagatcatg gttatcaagt ttattttgta
     8701 ctaaagacac caggctatgt cacaaagaaa agcaatttca aggtgatcga tgtagcaaaa
     8761 aaaatttcaa aaacaattcg tctacagttg gctgaaaaat tgtccggagt ggatctagga
     8821 tgtaaccact tcggaattgc tcgattccca caagaaaata atattgtttt taaagaaatg
     8881 aactatcagt attcttttgc tgactggatt caatggtcga tgaagcttga atcagatcaa
     8941 aaaacaaaag aagatcgaga acgaaaacta attgtctttc ctgagaaaaa agaaattcgt
     9001 caagtagatg aaccgtggtt cgatatgtta cttcataaag caaacatcgt cggcggagaa
     9061 ggacgtcttg gtcgaaataa tgtgattttc acgctttctt tagcttatta ctcttcaggt
     9121 tatgcattgg atacttgcga atacaatatg ttcacattca acgaacggtt acaggaaccg
     9181 ctatctgaag cagaattagt aaagattgta cgtagtgctt attctggtaa ttatcaagca
     9241 gcaagcagag aattcattat tgaattgtgc cagcaatggg tttcaccaac cttaaaaaat
     9301 aatgatctgt tcattcaacg acgtggctgg tggaaattta aaaagccacg agatcaaaga
     9361 aaatatagtc ataaaaatga atggaaagaa gatctattgg cttatctaag cgaaaaaagt
     9421 cttgctagtg agccttattt gaacctgtca aaaaaagaat tggcttcaca actaacgatg
     9481 cccaagcgaa cacttgataa gatcttaaat gagttacgag aggagaataa agtcttctac
     9541 aaggtaacac gaggaagaaa tggcggaatg atcattgctt cagtcaaaac gttgttcgcg
     9601 agggttattc aactaagtaa agaaaagaga ctagattatt caacaaggat cacagaggta
     9661 tttggactca acgagagctt agtagaagag acatttagac aattatcaac ccccttgaac
     9721 cctcttaaag aagtgaatct ttttgaagta gatacaggtt gaaaaataaa aggtgcaaat
     9781 tgccttatac aatatatcta tgatacttct gtaggtgttg ctccctttgc ctagctacgt
     9841 gtaggtggta tacttataac tgcgtatgca gtcttatgta tcatggagga agttgtatgt
     9901 cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt tatcattggt cgtttgggat
     9961 tatctgtgga agtgttctgt gagctttatg gttttaagca ggggactttg tcttcttggg
    10021 ttaccaggga aaaaacagtt gcttctttgc cgattgagtt tcttcatggg ttgtccttag
    10081 cttctgggga gactatggat caggtatatg attgtttgtg tgtgttagag caggagtata
    10141 ttgagtataa aatagctaac gagttaagaa aaaggaagaa gtatatccag tagtagtttt
    10201 tatttactta aaaaattggt tcattgagca acctatcgct gctaaagtag caaaggaaaa
    10261 cgttaaagga aagaatttag ggaagaaatc gaaatgaaat cggacgactg gattgaacac
    10321 acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat tttgggtatt gcaattatgg
    10381 ccattaggaa aactttgtag ttacggggca cgacttattc ttgatttaac cgtttactcg
    10441 attggagtat ttggaacggc ttattacttg ataaatgatc ccaataggat tgcagcatca
    10501 gacattttta cagtgctaat gacgttgatt tttgtcatgc tattgtctat ttttactcaa
    10561 ttagtcatgc aatttaccag gcaaaaatta acagcaggaa aacttttcaa aagcctattc
    10621 gatttaattg ttgtaaatgc aattctagcg actttaataa aacaaattga acaatctgga
    10681 agaacaacaa aaggagtgaa ggatttttta ttgacgttta ttcactttgt aaatggacac
    10741 atgtatttgt ttgaagtggg tattattctc ttttttgtcg ggtatgtatc cacgaaactt
    10801 attaaacgtt caattgaaaa agaaattgaa attgacctga ttccgacact tcttggttat
    10861 gagtacaatg ttgattttgg aagatatgac gaattgatca atacggatat cgttcacacg
    10921 agtatccatg aatctactgt taagggcatc agatctattt taaaaaatgg ccaggaaatg
    10981 aaagcaatag atcgaagaaa agagatctac aatttttatg aagtcgaagc aagcctacga
    11041 tatggcatcg gaaaattgtg gttagatgga agtaatattc gtcaaaacaa accatacatt
    11101 ttttggaaca gtagtgattt agaacttcca atgaaagata aataattatg gagaatcgtt
    11161 tgaaaatgat tcctctatac aggattgtgc ttatagttgg catggagtta aaaaccttaa
    11221 agaaggagca gttattcaaa gtgaaagaac gtcataatca gttactatgt agtaaaacgg
    11281 gtacgatgaa cattgaatac cgaacagaat atttagaatt aacgaatcaa actgattttt
    11341 tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg gctacttata gccaattgat
    11401 tgatgaatgg gaacaattca aggaatctaa aattccagaa cgtctctaaa agcctttatt
    11461 tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa aagtgttcca ataggctgtg
    11521 cctattacgt ttctcaaaaa taaatgtggg aacaaaatat ttaaaaatag gatagtgaaa
    11581 ggatagtgaa ggatagtaaa aggagggtag taggatagca aaaaggatag gctccttttc
    11641 gctatccttt ttaaattgga ggaattaata tggaagtaag aataagaaat gtggatgaga
    11701 aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DANKHJ010000085        11018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000085 DANKHJ010000000
VERSION     DANKHJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1019
                     /locus_tag="PW113_002497"
     CDS             <1..1019
                     /locus_tag="PW113_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2526767.1"
                     /translation="WIYFASIVFISILVTLLCFLGFIFLLSRHSISPAESAGKPTLYP
                     LFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQQKVAEVQQL
                     YKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLSDEERQIFLY
                     RIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQPKWEKEQLEL
                     DIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIVLEVTDSGVG
                     MNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIEGVGTTAMIR
                     LSKQ"
     gene            complement(1256..2137)
                     /locus_tag="PW113_002498"
     CDS             complement(1256..2137)
                     /locus_tag="PW113_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2526768.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            2296..3024
                     /locus_tag="PW113_002499"
     CDS             2296..3024
                     /locus_tag="PW113_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2526769.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            3180..3497
                     /locus_tag="PW113_002500"
     CDS             3180..3497
                     /locus_tag="PW113_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2526770.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            3490..4098
                     /locus_tag="PW113_002501"
     CDS             3490..4098
                     /locus_tag="PW113_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2526771.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            4153..4944
                     /locus_tag="PW113_002502"
     CDS             4153..4944
                     /locus_tag="PW113_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2526772.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            5082..5570
                     /locus_tag="PW113_002503"
     CDS             5082..5570
                     /locus_tag="PW113_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2526773.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            5621..6493
                     /locus_tag="PW113_002504"
     CDS             5621..6493
                     /locus_tag="PW113_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2526774.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            6669..7031
                     /locus_tag="PW113_002505"
     CDS             6669..7031
                     /locus_tag="PW113_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2526775.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            7040..7792
                     /locus_tag="PW113_002506"
     CDS             7040..7792
                     /locus_tag="PW113_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526776.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            7806..9608
                     /locus_tag="PW113_002507"
     CDS             7806..9608
                     /locus_tag="PW113_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526777.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            9885..10925
                     /locus_tag="PW113_002508"
     CDS             9885..10925
                     /locus_tag="PW113_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2526778.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         3590 a         1804 c         2330 g         3294 t
ORIGIN      
        1 tatggatcta ttttgctagt attgtcttca tttcgatctt agtgacactg ctgtgttttc
       61 tcggttttat tttcttattg tctcgacatt ctatctcgcc tgctgaatcg gctggaaaac
      121 caacgttata cccgttattc gtctttgcgg ggttcagtat ggtcgtgggt acaggaatat
      181 cgatttttgt tgggcggcgt atcctgcatc caatcagtgc gctagggaca aatatgagtc
      241 tggtagcaac gggagatttt tcgattcgaa tggatgaaca gcagaaagta gcagaagtac
      301 agcaactgta taaagatttc aacgttatgg tccaagaact caatagtatc gagacactga
      361 ggaatgactt tgtgtccagt gtatcccatg aattcaaaac accactagca acaatccaag
      421 gatatgttca attgttacaa gccccaaatc tctctgacga agaacgccaa atatttttat
      481 atcggattat cgaaagtatc acgcagcttt cacaattaac agagaacatc ttaaaattga
      541 acaaactgga aaaccagcgt atccaactgg aaaagaaaga gtatcgatta gatgaacaaa
      601 tcagagaagt aatcgttttt tttcagccta aatgggagaa agaacagcta gaattggata
      661 tcgaacttgc tgcagtaaat tacacaggca atgaagagtt tttgtaccaa gtgtggctga
      721 atatcatgga taacgccata aagtacaatc aaatcaatgg acagattcat atcaaactat
      781 ttgagacagc cacagagatt gtactggaag tgaccgattc aggtgtagga atgaatgaag
      841 agaccagaga tcgaatgttt gaaaaatttt atcaaggaga tacaagtcgt caaatttctg
      901 ggaatgggct aggactttca ctggttaaaa aaattctaga actgcatgat ggacgtattg
      961 attatagcag tatcgaagga gtaggaacga cagcaatgat acggttaagt aaacaatagg
     1021 aaaaagaata aaacccctaa aaattctatt gaaggttttt aggggttttt agtcgttttt
     1081 ttgtgattag ggaaatgttc ttacggttct tttggcataa aaatcgctgc tttcttattg
     1141 aactacgaga aatgttagag gtgtcattga aacaaggaca tgatgcaaga aagagagcga
     1201 aagtcatgcg tcgctctctt tttttgggtg gatagatgga cagaaaatgt agggcttagt
     1261 tttcttcttg atttaaatac tcccaaaatg tatctgaagc caaagaatgc tcaccgactt
     1321 tacgagtaaa tgcaagataa ctctctgttg ttggagaaat agggagaatc acagtatttt
     1381 cttttaatag attcttgact gttttttcca taacaatagc aatccccatg tcatttgcaa
     1441 tcatattgat gatcaagtcg attcttgctc ctttataaga gatttttggt tcaaatccag
     1501 cttcgcgaca cagatctagt acaggttgat acaagttcgt agtggggcca aggagcaaaa
     1561 agttctcctt tttcagttcg cttagatcca atttcttttt actagctaaa ggatgatttt
     1621 taggtagaac tgctacaaaa tcgtctgttt cagtaggcaa ccactcaatc gaacttggtg
     1681 gggcctggaa acttctagca aagtggatcg tgtcactatg ttcattggcg tcattgtaag
     1741 ttaattcgac actttccact tctttcaact ggactgtaaa ttcaggatgg cgtttcaaaa
     1801 actcggtgat tttggaaaaa cctttataat tgatcattgt cggtatcgtt aagatattta
     1861 atgaaagatt gttgtctttc gtataggttt caagcacttc ttgcatagca tggtattgtt
     1921 ctacgacatt ttttacatat gggagcaata agcttccagc ttcggtcaat tcgatttttc
     1981 tatgggcgcg ttcgaataat ttgacgtcta attctgtttc taacgaaaga atctgtttag
     2041 aaatatttcc ttgggtggtg aattgtcgat cagcagtttt ggtgaaattc attgtttcgg
     2101 ctaaatcaag aaaaacttcg aatttatgga tatccatcat attcctccta cttattccaa
     2161 taaagaatac tttaacacaa aaaatgaatt ggtgtaatct tctctgtctg ttacaatctt
     2221 tcttgcagcg aaagcaataa ttacagaaat ttttctgatt tttttcgctt cgtagaaaaa
     2281 gagaggagtt ttttgatgcc aactggagtt ttgatcaatg taggttccgt tttgtttggc
     2341 ggactaattg gcggattagt aggaaataaa ttaagtgaac attttaaagc acagctgacg
     2401 atggtttttg gtgtgtgttc gatggggatg ggaatctatt ccattgcgcc gatgaaaaat
     2461 atgccggctg ttatttttgc tttagtgata ggaacagcga ttggtttgat tgttcattta
     2521 ggtaatggga taaataaggg ggctgcgctg atgcaagtac ctatttctaa aatcttccct
     2581 agtgaaaagc ttggtatgac ccacgatgaa tttatcagca cattagtgac ggtcattgtt
     2641 ctattttgtg caagtggtac ggggatctat ggaagtttag attctgggat gacaggagac
     2701 agtacgattt tgatttctaa atctgtcctt gattttttca cagcagctat ttttgcgtgt
     2761 aatttagggt atgtcgtttc tgtgatagca attccccaat tcattatttt ttatattttg
     2821 ttcttattgg caaaatttat ttatccattg actacaccag acatgatctt agattttaaa
     2881 gcttgcggcg gattcttgat ggtcgctacc ggttttagaa tgatcaacgt gaaaatgttc
     2941 ccagtagctg acatgatacc tgcaatgatc gttatcatgc cactgagctg gatgtggaca
     3001 aattggatta tgccactgtt ataataaaaa tcaaagagaa gccatttttt gctgactaaa
     3061 aaggatcaaa tcttttagtc agtttttttg tatttgacaa actgattttg aaactatatt
     3121 atatgttgta taatatagat tgaaataaaa tattacactt gagaatagaa ggtgacgtaa
     3181 tgcacatcca gattccaact gttcttcttg atggtactgt gctagcagca ttaagcaagg
     3241 aagatatgta tgggtatgcc ttaacaaaaa tcgtacaaga aagtctctct gtcagtgaat
     3301 ctacactata tccggttttg agaagattga aaaaagaagg atatcttgag acctatgatg
     3361 aaccttttga aggcagaaac cgtcgatatt actcgttgac accaatggga agtcgtcatt
     3421 tagaagaaat tattgatgaa tggattgttt ttcgagattc tttagaccgg ttattggagg
     3481 agagcacaaa tgaatgaata tttgaatgaa gttgtggatc atcttcaaga aattcctgaa
     3541 gacgatcgtt ttgatttgat ccagtattat gaggaatatt ttttagattc tggaaaaaca
     3601 ttagatcaga tcattgatga atatggtaca ccaaagcagt ttgccttaaa attaaaaatc
     3661 agttactttt cagaagaaga acaagcagaa aacgagttag tgaaaaatcc aaaaagacaa
     3721 attcgcctga tttggttaat cattatcggt ttatttgctt ctcctgtcct cattccacta
     3781 gcactggggt ttatattggt tattggggga ctattggttg ctttactagc gctcattttt
     3841 agtatctatg ttttatgtat cagtatctta ggtgttggcc tattcatgat catctcagga
     3901 atcagtgtgt ttggacaatc gattctgagt ggattattct ttatcggtgt cggtctgttc
     3961 gccacaggaa gcgcagtatt tatttcgcca ctgttgctga aaggaacaaa agggcttttt
     4021 tatgtcatga tgaactttgt taaatggatg ggacgacgtt ttattacaaa aagagatctg
     4081 cattcagcgg gcgtttgaag gaggacacat aaattgagat tgaaacattt tttgattacc
     4141 ggtattggtc tgatgcttac tggtggcctc ctagcaggtg caacttatac attaggcgct
     4201 caaaagagtc tgacttggga taatggtcct cggttgatcg atatggttac tgagacaaaa
     4261 aaaatcaagg atgcaaacaa aatcgtcatc catgcagaaa atcaaacagt ttcggtcata
     4321 cgcggattgg attttgagat aaaaacgact tatgataaga cagaaaagcc agaaataaag
     4381 gagaaaaatg gcgtgttgac tgtgaatgca gtcaaaaaat caacaggtgc aatcattagt
     4441 ttagaagaca caagtgcaca aatcactatt gttgtccctg cagatcatga attatctgaa
     4501 ttgacaatcg aagggaataa tagtgcaatt gatatcaacg aagtatcaat caaaaaagtg
     4561 aatactaaga cacatgatgg ctgggtatcg ttcaatgaat ttacaggagg aacaataact
     4621 gcgaaagatg aattaggctc acttcgatta gatgaggcca atgtggataa cttgatcgtc
     4681 acaggtgaaa acagcaactt ccatctagaa gacagtttga tcagcaaaga aggaaaagtc
     4741 atcctaaacg gtggcaattt atctttgcat gagatggaaa acagtggcta tactatccaa
     4801 acgtctggtg aatcttatat tgaaaaagac taccagccaa taacaaatac tttgttagaa
     4861 caaggacaga aaaagctaaa gatcactgct aaagacacat cgatcaatat tacgacggat
     4921 caagaggaca atgaaacaga ttgatcctgt actcaactgg atattttaag aaaaaacagc
     4981 aaagatcctt ccaatctttg ctgtttttca tcgcttaacg cacataacta tagtaaaata
     5041 agaaagaaaa tatctaaaaa gaaaacaggg tgaaagagta tatgaaggta gaagaaggtc
     5101 aaagagaaaa gttaaaaaca gaaatggaaa gattacatac ttatatcact caactaagcc
     5161 aaaccttcta tgatccagac aaagaaaaag tgatggttaa ttacccgaac aattcagaag
     5221 gtcgccaatt agagcaagtg taccatgaag ttttcaaaca tttattgacg gtgaagaaag
     5281 agctggatta ttattcttta ccgattatcg atacaggaat attgaagtat gatgggaaga
     5341 aggaacgatt tatctttaaa tctgttcggg aagatttgcc tctttcagca gggatggatc
     5401 tagaggtctt ggtagaagac tattttacag aagaaaagca ctgggttcga acaaaattgg
     5461 attatttacc gcaggcagcc ggagggacac aggcaagcgg ttggtacatc actgaagaca
     5521 aagagttaga actagaaggt gtaatggcgc gtattcgaaa aaaaaattaa aaaagtaaca
     5581 ttagatattt attgaaggat aaaggagcgc aggaagtagc atgagagaaa aaagagcatt
     5641 ttatatcaat ggttatgcag ggattgtcgg attggtcgta ttagctttag ttggattgtt
     5701 tttcttctat ttaggtatgt ggcaagcgaa agtttgggca ctttttttaa gtatttttct
     5761 ttggttgatc acgttattgt tgttaagttc ggcaactgta gtcagtccta atcaagcaaa
     5821 agtaattttg ttttttggac aatatttagg tacgattcga gaaaatggct ttttccttac
     5881 gatacctttg gcacaaaaaa tgactgtttc gttgaaagtt cggaatttca atagttctgt
     5941 tttaaaagtc aatgatttgg atggaaaccc catcgaaatc tcagcagtag tagtctttaa
     6001 agtcgttgat acagcaaagg cattattcga tgtagcgtat tatcaagatt ttgtagaaat
     6061 ccaaagtgag acagccattc ggcatatcgc tagccaatac ccgtatgata cattcaatga
     6121 gaatgatttg acgcttcgag gaaacacgac ggcagtgtct gacgagttgc aaaaagagct
     6181 gcaagagcgt ttagcagtag caggtgtcga agtaatcgaa acacgcttga accatttagc
     6241 ctatgcaaca gaaatcgcta gtgccatgct ccaacgtcaa caagcaaaag ccattttgtc
     6301 tgctcgacaa accattgttg aaggagctgt cacaatcact caaatggcct tagagcaaat
     6361 cgaagatgga caagaaatca atttcaccga tgatcggaaa gtccaattga tcaataattt
     6421 acttgtctca atcattacag acaaaggaac gcaacctgtt atcaatacag gcgacatgaa
     6481 agaccgtaag tgacatactt tttgtaagat aggaagaagg agtttaggac ggattattgt
     6541 cttgaactct ttttttagtt gttttaaaga aaatgacaaa attgaaagaa gaatgatagt
     6601 tgattcgaag gcagaattgt tcgctttttt ctataatcag tatataaagt aagagagaag
     6661 gggaaaaagt gaaaaaactt ctgatcggtc tacttgtttt catcgttgtg gtacttgcag
     6721 gggtgaaaat cgcagattac gtagttatgg gtggagaaga atactatgta caaattacga
     6781 cagatggtac aaaaacagtt tcaaaatcag actctggaca agaaacagtc aactatagat
     6841 atatcctgcc tggctatacg aaagagggta aggaaaaaga actggacttc aagggacaat
     6901 tgactcgacc gcttcgtaaa ggagcatatt taaaaatcac ctggaacaaa aataaaggtg
     6961 tcacttcgta tgaagaagtc gaaaaaaaag acgtgccgaa aacagcactg gaaaaactaa
     7021 ttaaagggta gggaaagaaa tgaacaaaat agtcgaagta cagaatgtaa caaaagtata
     7081 cggaaaaaac aatgagaaaa agacgcaagc attaagcggc atcagcttcg atgtagaaaa
     7141 aggcgaattt attgggatca tgggcgcttc tggttctggt aagtcgacac ttttgaatat
     7201 tttatccaca ttagataaac caacagatgg gcatatccga atcaatcaaa aagatgtgac
     7261 cacattaaaa ggaaatcaac tagcagattt tcgtgcgaat gagattggct ttattttcca
     7321 agatttcaat ttattagaaa gcttgacagc acaagaaaat atcgctgttc ctctttcttt
     7381 acaaggcgtc agaccaaaag aaatcaagca acgtgtccaa aaaattgcag aacgtttatc
     7441 catctctcat attttggaaa gctatccttc tgagatttcc ggtggacaaa aacaacgggt
     7501 cgccgccgca cgtgcattga tcacacaacc aacgattcta ttaggggatg agcctacagg
     7561 tgcgctggat tccaaaagtg cccgggatct tttagatacg atggacgaat tgaatacaaa
     7621 agatcatgta tcgattcttc ttgtgacgca tgatccgttt tctgcaagtt attgccaacg
     7681 tattttattc atcaaggatg gcggtatcca tcaagaagtg aaacgtggag atcaaagccg
     7741 tgattccttc tatcgagaaa tactatctat tcttggaaac ttggaacagt agggggagat
     7801 tgactatgct ttggaaatta tcacttactg ggatcaagag ccgtttaaga gactacttgg
     7861 tgttattctc aggattagtt atggcatctg ctatttttta catgtttgaa tcaatggcaa
     7921 gcaacgaagc ttttttgaaa agtaatacga tcgttgcggc tacagttatc attttccgat
     7981 tcggttctgt tttactaggg atcatcacat tcgtttatat tttatatgca aattcctttt
     8041 tgatgacgat gcggcaaaaa gactatgcaa tgtttatgat gctgggagca aaaggcagaa
     8101 aaatcgcaca gatgattttt tctgaaacat tcttagtagg gatcgcagct actttagtgg
     8161 gttcagctgt cggtgtagga ttgacaagta tcgtccaccg attattagtg gatcagttag
     8221 atctcaatat ttcgcatttt acacctttta gtattcacgg gttgttgatc actgttttgt
     8281 tttttgctgt tttgttttta ttagctgctg ttgtcaatgc tttttcgatt gtcaaaaaac
     8341 cgattttagc attgattcgt gcggatcaga caccaacaag aatcaagcag cataaattct
     8401 tgtttttcct tgaagtagta ctgggaattg cttttctagg ctgcggttac tatatgatga
     8461 aacacgtggg gacctatcaa gtctttggta taggggtagc cttagttacg atcgtattag
     8521 gaacttattt cattttccat tctgtgatca ttttcttttt atcattacta aagaaatcag
     8581 aacagatttc attgaaaaaa ttgaacaatt tcacattgtc acagttaaac tttcgtattc
     8641 gtgaatatac acaaatgctc tcaatggtcg ctatgctgtt tgccttggca ctgggcgctt
     8701 tgacggtagg attaggtttt aaaaatgaaa ttgcagactt gacagaaaac aattctggat
     8761 atgatctagt gatgaataat cctcgggcag aagatcagca aaaaatcaaa gaactttcac
     8821 caacactttc tgcttcttat actcaaaaag aagatgcggg aacgatttac tataatgcaa
     8881 gtgaatttgc ggctcaacca ttgatcatga taaaacatga agatggaaca ccgccgaaga
     8941 tcacgaaaga aaaagttcct gctaaaaaaa tgaatgaact gactgtacaa gaggaacttc
     9001 gaagctatga attgccggaa cagcaagaaa aagaaatcaa ggtagtcagt caatcagaat
     9061 ttgatcaact gaacttgcca caatccactt tacagctagt tcaggtaaaa gacttccaag
     9121 caaacctgaa accaatcgaa acacttgttg ctcaaaataa aatgaacaat ccatctttgc
     9181 aaaaagtaga gtacagcaac cagaaatatg aaatgtatga agtgtataat ggtctattct
     9241 ctggttttga atttatggga ttcttcctag gtttagcttt cttgaccatg cttgctagct
     9301 gtttgatgtt caagatcttg tcaggaagca agagtgacat tgctcgttat gaaatgcttg
     9361 aaaagatagg aacacgacgc gggttgctta aacaatcgat ccgtagagaa gtaggcgtgt
     9421 tattcttagc accaggaatc ttaggtgtag tccatgtctt attcggatta caaatgtttg
     9481 gtctattcat gcaaaatcca tacaaagata tttgggttcc gtttacgata ttcttcgtat
     9541 tgtatttcat ctactatgta ctgacgacat ggttatatac tggaatcgta ttgaaaaaga
     9601 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaagcaaaa atagctcctc
     9661 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttgttc
     9721 ggaaaaagag gttgtgacaa tatttttgtc acaacctttt tttcacggtc ttttattaga
     9781 atatagtaaa ttttttctcg ttactcacgg aataagctgt taagaaaacg gtttccatgt
     9841 atactggaat cagtgcaaat gaaagaaaag agggtgcaag gaacatggaa atcaatgaac
     9901 aaaattttgg cgaaaactat cacttgatta cgatcacgaa tcaaaatggg acatctttgt
     9961 ctgtcagtga tcttggggca cgggttgttc attttcaaac aataatcgat aatgaaaaac
    10021 gagagttgat cttaggtttt gatgaagcaa aagaatatct agaaaaagac ccttatatcg
    10081 gtgcttcaat cggacgtatg gctggacgga ttacgaatgg cacattcaaa ttagatgatg
    10141 aaacgttcga agtaaaaaca gacccggaca ctggtcattg tttacatggt ggtgcaccta
    10201 gctttgaagc caagcagtgg cattatacag tcatcaatgg tgaaaacgaa gcatcggtca
    10261 ttttctatca gactagccca gatatggaaa acggatttcc tggaacttta gatgtagaag
    10321 tacggtatac gctgacgaac gatgatacat ggcgagtgac ggtccaaggg atcagtgacc
    10381 gcaaaacagt attcaatcct acaaatcatg tctatttcaa tttaaccggt gatccagcac
    10441 agtcaatcga cgaacatata ctttggttga atagccgatt atttgctacg ctcaataaag
    10501 atacaacgcc tacaggagaa gtagtttcag taagtggcac agcatttgac ttccaacgtc
    10561 ctaaaaaatt aggtgaggtc tttgccagtg attttcgcca atcaatgata gtgggcggca
    10621 tcgatcatcc atttttgttg aaaaaacaat ctggtttact aaatgctgcg gaattgacaa
    10681 gcccagatga aaaagtatcg atccaagtaa aaacagatgc ttcaggaatc gtgattttta
    10741 cagcaaattt tggagaagat ggaccggaaa tgcgcgggaa taaattagta aatcatggcg
    10801 gtatcacatt tgagactcaa gaactgccag gagcggagcg acttgaaagt tttggagatg
    10861 tcactctgga acctaaccaa gtacgcgagt ttgtaacgga atacaaaata aatataaaga
    10921 aatagaataa attaaaaagg gagtgtgaca gtgtcacatc ccctttttaa tttattctat
    10981 ttctttaaag gcagatcaag ccagaatgat ttgtctaa
//
LOCUS       DANKHJ010000086        10852 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000086 DANKHJ010000000
VERSION     DANKHJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            1..329
                     /locus_tag="PW113_002509"
                     /pseudo
     CDS             1..329
                     /locus_tag="PW113_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(231..947)
                     /locus_tag="PW113_002510"
     CDS             complement(231..947)
                     /locus_tag="PW113_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2526779.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(997..1206)
                     /locus_tag="PW113_002511"
     CDS             complement(997..1206)
                     /locus_tag="PW113_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526780.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1483..2756
                     /locus_tag="PW113_002512"
                     /pseudo
     CDS             1483..2756
                     /locus_tag="PW113_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2863..3894)
                     /locus_tag="PW113_002513"
     CDS             complement(2863..3894)
                     /locus_tag="PW113_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526781.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3901..4731)
                     /locus_tag="PW113_002514"
     CDS             complement(3901..4731)
                     /locus_tag="PW113_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526782.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4728..5543)
                     /locus_tag="PW113_002515"
     CDS             complement(4728..5543)
                     /locus_tag="PW113_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2526783.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5540..6364)
                     /locus_tag="PW113_002516"
     CDS             complement(5540..6364)
                     /locus_tag="PW113_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2526784.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6354..7454)
                     /locus_tag="PW113_002517"
     CDS             complement(6354..7454)
                     /locus_tag="PW113_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2526785.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7632..8417
                     /locus_tag="PW113_002518"
     CDS             7632..8417
                     /locus_tag="PW113_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2526786.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8450..8833
                     /locus_tag="PW113_002519"
     CDS             8450..8833
                     /locus_tag="PW113_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2526787.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8909..9040
                     /locus_tag="PW113_002520"
     CDS             8909..9040
                     /locus_tag="PW113_002520"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2526788.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9009..9245
                     /locus_tag="PW113_002521"
     CDS             9009..9245
                     /locus_tag="PW113_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2526789.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9323..10294)
                     /locus_tag="PW113_002522"
     CDS             complement(9323..10294)
                     /locus_tag="PW113_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2526790.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3231 a         2522 c         2071 g         3028 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctatgctatg attgtgggca gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa acaggcgatt ctcatagaac tgtacgaaaa tcaatcacgt
      181 aatctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc ttactcaaag
      241 attccacgac attggagccg ctcaaacggc attatcatcg actgtttaaa cgaccaatta
      301 gtcaaacaga aatcgtcgat gaaccgttgt gcacaatggt gtcctgaacg tacagcgaca
      361 ccttccatat ccagagcagt cgccacatca cggggatgga tgccatccaa attaaaagca
      421 accacgccag tatgatgttc aggatcttgc ggtccgtaaa tggtgatacc gccgatcgct
      481 tgtaatttag gtaaaacgta ggcaaccagt tcttgctcat aacgatgaat attctccata
      541 ccaatctcat tcaagtaatc gattgctcct gcaatgttcg gagtaccagc ttcaaatttc
      601 catggcagct ctttccatga actatcgtaa agattgacaa aatctatcat ttcaccgccg
      661 aattcgactg gctccatttg ttctaacaat tgacgtttgc catataatac accgattcct
      721 gtcggcccgc acattttatg cccgctaaag gcgaaaaaat ctgcatccaa cgcttgtaca
      781 tcaactgtca tatgcggtgc tgcttgggca ccgactggac tatcctcaac tacaacagcc
      841 tgttcaaccc tcagccgttt caagcacaac aaataacctt ctggatctgg tttgccgacc
      901 tgaagatcat cgatcgtcac aaaggaatca attttttcct taatcatcgg atgtgcttca
      961 aaaaaatagt ctagctcccg cagttcagaa ttgagactac tccgattttc cagtgctgct
     1021 gatccgctgc tcaaaatgtt cagcatccac tggtctccca accgcttgac ttatcccttc
     1081 ataaaaggac agttctgctg ccgtcccaac accttgttga tagtctgcaa aaggaatttt
     1141 atagccgaac tgctccagta aataggcttc agtaaatagt gatgaatact ctcgtaaaac
     1201 atccattcac tgtcaatcat cgtcccatca aaatcaaaaa taagtgcttt ggtcatcttc
     1261 tttccccctt acccaaaaca agcaaaagct gtgtcctgct ctctctacag tatcagcata
     1321 aaaactgccg tctgaaaagt caaaggtgaa agaaatgtgg cgctataatt tctaggactg
     1381 atagtaaatc attctatcag tccttttgag cataaaaaaa caaaacatac agaagctccg
     1441 ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatctt
     1501 atcaaactat tactaggaat caaagatgga aacattcaaa tcactcatgt cgagaagaaa
     1561 aaaatacatt caatcaacag tatcgttgtt tctgccacgc tcattaaaac gattcagccc
     1621 tgtcctcagt gtaaacaggt gaatcaacat gggctgatcg ttaagaacgg aaagaaaaaa
     1681 tcgctggtcc ttctcaataa gtgtgcgaat caacggactt atcttgcttt ggcaaagcaa
     1741 cggtacctct gtcgtggctg tcacacctat tttacggcga atacctacat tgttgaaaaa
     1801 aactgtttta tcgccaaaca agtacattat aaaatattgg aggaattgac tgaaaaacaa
     1861 gcaatgacga cgatttctaa acattgcggc gtttcttggt caaccgtttc gcgaacactc
     1921 gcttctctgc tccccatgac aaacgtgaaa ataaattggt tgcctcgctg ttcattggta
     1981 gatgaatttc gttcattgaa aaatcaagtc ggtccctatt ctttttcttg tatggatggc
     2041 gatactggga aattactgga tattcttcct tcacgtaaga agaaagatct catttcttat
     2101 tttatgcaat ttgaacgacg tgcacgttta aacgtaaaaa tactcgtaac agatatgaac
     2161 gcctcttatg ctttgctgat aaaagagtgc tttcctcatg ccaaactcgt tgtggatcgc
     2221 tttcacatcg tcaagcacct cattcgcagt tttaaggata tttgactatg agtcatgaaa
     2281 agcttcgacc gaaacgatcc aatacaagcc aaacactatc gacaagtgaa ggctttatct
     2341 cgacttctga tcaaacgaca ggacacgctt gtttatgata aatggacaaa atggcgtaat
     2401 tttggttggg cctatttaac cgaaagtgaa gtcgtagaac gtctactttc tacttctgat
     2461 gaactacgaa tagcttatgc ttactatcaa gaaattcttc aagcttttta tgacaaagaa
     2521 gcggacatct tctttcagct tgtaaaaaca atgcccaagt cggttccaag agaactgcat
     2581 catataaaaa aagcgtttat aaactatgaa tccggcattc gtttagcctt agagttgcct
     2641 tactcaaatg gcaaaataga aaatctgcat acccacatca aagcattgaa acgaatagct
     2701 tacgggttag gatgagaacc agaattttct taatcaatca tctgatcact tattaaacat
     2761 aaaaaaacat ctaaagcaga aaatcaattc taccttagat gttcttatat cactctatca
     2821 gtcctatttg acaaagagcc agaaatgttt gccgactata cactagcttt gttgtgcaaa
     2881 atgctgagta tcaatttcca cagggattgc tagaatatct ttcagctgaa tgtataaggc
     2941 atcgctaatt ttctcttccc gatcgttcaa ccgatccact aaaatttctt tgccctccag
     3001 cgtttgaaaa acataccgtt gaatgttgcc taaaataaat gattccttcc atagaaccgg
     3061 aatcttaacc ccctgaggaa ccggttcaaa ttgaatcagc tccggtctta aataataaat
     3121 gcagcgattg cccttagcgg aaaactcgtg ggtatactga ttcagctcat accattcgaa
     3181 acgattatag ttgccgataa attccgcaac aaatcggctc tgaggattct gatacaattc
     3241 cttcgaggtg gaaacctgag ccacccgccc agcttccatc acaaaaatcc gatcactgat
     3301 tcgcatcgcc tcttcttggt catgagtcac aaaaatcatc gtcatcccta gcgattgctg
     3361 gatagcgcgc aaatcccgct gcagctgttt tctaattcgg gcatccaaag cacttaatgg
     3421 ctcatccagc agcaggactt tcggttcagt gaccaatgag cgagccagtg ccacccgttg
     3481 tcgttgaccg ccagaaagaa actggacatt tttatctgcc tgctccgtca tatccaccaa
     3541 tgatagcatc tcaaaaacct tttcctgaat cacctcagga ctcagcttct ttaccttcaa
     3601 accaaaggct acattttcaa aaacagtcat agttggaaac aatgcatact gttgaaaaac
     3661 aaagccgata ttgcgatcct tcgtaggctt atcggcaata ttctcattgt ccagcacaat
     3721 ctcaccgctc tttggttgtt ccaatcccgt gatacagcgc agcaacgtag actttccgca
     3781 cccgctggga cctagtaaag taaccagctc acccttttca atctgaattt catcaatagc
     3841 taaaatctgt ttttcatcaa aaaacagatt gagatcatgt atttctaaaa acattttccg
     3901 tcacaactcc tttaagggct tctttttcga ctgcttctct tccctcgtct gcttaaggga
     3961 cagctgttgc ttttgtcgat aagtcagcat aaaggtagtt aaagcaattc ccaccataaa
     4021 cagcaaatac aaaaccatca cagcgctagc caaatggccg ttttcgttca tccgccgcag
     4081 catatagatg cgtaccgtct gaaatcgacc gccaatcaac aggttcgtca gcatgtactc
     4141 cccaaaaacc gaagaaaaaa tcatcagaga aactagggtc acaccgatac ggatgtttgg
     4201 aaacagcacc tggataataa tcgtagacat cggggcgccc aaggtttctg ctgcatccac
     4261 taattccttc agattaatta agcgcatctg gttgttaaga ctaagataga caatcggcaa
     4321 cgccgtaata aacaatgcac ctaccaacac tacaaacatt gggataccag tttcagcata
     4381 cgttcgaagc aaagccgtta ccaaaatcac tcctggtaaa gcatacggca gcaggagcag
     4441 cttttccagc caacgattca aacgagggaa atacaaatga atccaaataa tggtgggaat
     4501 catcaacaac agaatcgtca ccaagaccac acctgctaat aaaagtgagc gacccaccgc
     4561 agcaataaat tccctgtccg tcagcagctg ttggaaccat tgcaaggtaa aatcactagg
     4621 taaaagcgat tttgaccatt tagtggagct ggcatacaaa atcgttccaa taatcggcag
     4681 caagtaatac aggacaaagc agaacaaaat cggttttatt aactttttca cgtctgcacc
     4741 accttgcctt tcttttgaat aatcaacatt gccgttacca gcaggacacc taaagcgact
     4801 gccaacgcac tgcctaaatt aggcttggcg aacacatctc ccgacactaa agccgcaatt
     4861 cgaatcgtta aaagatttac attactgctg gtcagggcat acgctgtttc ataggtcccc
     4921 atgccattag caaataagat gacaaaggtg ctggcaatcg atggtcgcaa aaaaggcaaa
     4981 gccaccttga accaagcata cgctgatgaa gcgcccagca attgtgcgga ctccagccat
     5041 tcctttttca cttccttcat gctgggatac aaaaacaaga cccctaacgg aatttggaaa
     5101 taggtatacg tgatggtcaa tccccaccaa gaatacaaat tgaagtccgc tagaaatggc
     5161 aaaatcattt tcagcacacc tacattcccc aacaaaataa taaaagagaa tgccagcgga
     5221 atcccagcaa aattagctgc caaattggaa acaaaggtga ttttctcctg caatttagtc
     5281 gatagattca ttaagcataa acataaaata aacgtcccga ttaacccaat cacactggag
     5341 ctgacagcaa tcagtacgct gttaacaaag gcctgtgtgt aatatttgtt gctgaaaata
     5401 tcactgtaat tggccaatgt ccactggtta gttaaatcac tgtgcaagct gcacagaacc
     5461 ataaaaagca gcggcagcag taaaaatgca cagaagataa aggaggcagg aaccagaacc
     5521 aagcgttttt tcatctcttt catcccctca tcaattcttg aatgtcctgc attttctctg
     5581 acggtgcaat tcctaataac cagcaaaaca atggagccgc cagcagctgt ggtacagttt
     5641 gttccttcag cttttggcgt ttatttgaaa tcaaataaaa aggaacttca cgatgtgcag
     5701 ccaagctgcc gccatgcagt ccgtgcgcat ccatcccgtg atccgcagtc accacgactt
     5761 gataaccttt ttgcagccat tcaggaaggt aaatagccaa taatctgtcc gcctgattaa
     5821 ccgctgctgc atattcatga gaatcggcgg taaatttgtg accgacatca tcaatattca
     5881 tcgaatgtac gaccaaaaag tctggctgat actgattcac taaataatgg gcatccgcaa
     5941 ataaatgcga atcagggtaa gtgtcctcac tataaaagat gccatgctga atcagtcgct
     6001 cctcctgatg ctggatgcga tcaaccaaac gattataggg ggcttgattg tacaactcgc
     6061 tgtaccagtg gtaagcggcg gctcctgttt ttccgccagc ttccttaact aattcaaaca
     6121 gcgaccactc tttggatcgc tgagcatggt aattactata gatgccattt tcataggctg
     6181 gaacgccggt catcagtact tcgtataacg gacgtgaatt gctgggcagc tcacttttta
     6241 ccttcaataa gcttgcttgc tggtgctcaa ctaaatgatt catgaaccca agctgggcga
     6301 cggctgtgtc gtagcgacag ccgtctagta taactacaat cagtttgtca agtctacttt
     6361 gcataggtca gtacctccgc ctgccaaagc tctggaagct ctacagaggt cttgttccaa
     6421 gcttcattgt cttcaactgg caccgccgct tcataagcat cctccggcag taatttttca
     6481 gctacttctt taggcagctc taccttgcga ataggtcgcg catagccctt tgccaaattg
     6541 atttgccctt catcagataa aatatattcc cgtgctagct ttgccgcatt gggatgaggg
     6601 gcatttttat tgattaaggt tgtatacccg ctaatgatgg agccgtctgt tggcacagtt
     6661 acctcaaaac gctcggagtc aatttggtca cgataattta gtccattgaa gtcccaaaca
     6721 actgccacat cgatttcacc tttttcaaga tttgctacgc tggcatcaac ggtggacaaa
     6781 cggccttttt ctgccatttt tttgtagtaa ttcagacctg gctgaatatc cttttcgtca
     6841 cctccgttgg caatcgccgc agccaataca gcaaattgtg cttggttggc aatcgtcaca
     6901 tcccccacgc taacttgata gtcaccctct tgcagctcct tccagctagt cggtgcttgc
     6961 ttcacattct ctttatcggt gataaaggaa atggttcccg tatagcttaa caaccaatcc
     7021 ccatcatcat cctttgccca atccggaatt tcctcccaat agttggtctt ataaggtaag
     7081 gttaactctt tgctttcagc caacggaccg aagctgatac cgacatcacc aatatcggcg
     7141 gttccatttt ttccttctga ttcaaactta gccaattctt ctgcactgga catatccgta
     7201 tccacgtgac ttaagccgta ttctttttca aggtcgttcc aagtgcctac ccaatttgcc
     7261 caagtatccg gcattcccaa gctggcaatc tctccttctt ccttggcttt ttctataatc
     7321 gtatctaaag agtcgtctgc cgcagccgat tgcttcgttg cttcacctga gccgcagcct
     7381 gtcagcagga tacttactgt tgctgcacaa aataaaccga tagtcatgtt acgaattttt
     7441 tcattcattt tcattcattc ttctcccctt tcgataattc cattctaaca aagctttgta
     7501 atgagagaat cagtttcttg aaaaggtttc atgaacaatg taaaggttgt atatggtttg
     7561 taaatttcga cgaattcccg ttaaccttct aaagcttttg cggtatgata gtagaaaaaa
     7621 ggaggctccc tatgcagtat tatgatcagc atctccacac gtatttttcc tttgattcag
     7681 aggaaaaatt tgaaaactat ttagcctatc agccggagtt tttcgtttct acggatcact
     7741 ttgatttaaa caatccatct accggaaaag atgacattcc ggattatcca gcctatataa
     7801 acaaactgga ctctttaaaa gaaacctatt ccaccaaatt tctgcgaggt atcgagattg
     7861 gcgttgttcc aggacaagaa gagcggatcg tggattacct cgctcagcat ccttatgatt
     7921 taaagctggt aagtattcat caaaatggcc gctttgatta tatggacgat gtggttttaa
     7981 ccaaagatcc cattcaaact accaaggaat attttgatca gatgttgcag gtcttgacgg
     8041 attttccaga aggagatatt ttgactcact ttgattatgg attacgccgc ttcaccttca
     8101 cagcggaaga attcgcggag cattttgagg ctcagctgct aacgattttc aaaaaggttg
     8161 ttgaacgaga attggcgatg gagttaaatg ccaagagctt cttgaaatac ggcaacgaag
     8221 acctttaccg ttatgccgtt cctttgtatc aaagtgtcgg cggaaagctc tttaccttag
     8281 gctcggatgc tcatgttgcc gaagattatc agctgggctt taaaaaaatg agtcagctgt
     8341 tggcagactg tcaggtagaa aagttgctag tcattcaagg aaaagaacgc cgcctgacgc
     8401 cgctgccgat tttgtgattc ctatataata gaagaaagtg gtgaaaaaaa tgagcaacct
     8461 gtgggactac aatcaagagg cacccatcca ttatttaatt gcgcgacatt gggatgcgtt
     8521 gaaaattgag gcggtttgcc gatccttgtt agcagccgtt ccaaaacaac aactggaaaa
     8581 ttttttggtg gctgacagct tgcagcggga aaaagttcag gcttattttg cagcctttaa
     8641 agatcagcct ttggaatatt tgcatgcgca gtttcatctt ttttatcaag tagccgcccc
     8701 agatgactac aacgatttac gaggacagct gcaattgacc tttcaagcgg atgaaaccgc
     8761 ctataccgtt ttattgggaa tggctcgcct tggtgatcag gcgaaggtcg agtggcggat
     8821 tttcgatatt tgatggcagg atcaaggcgt ttcagttgca aaccttcctc tctttcaatt
     8881 agaaaaaccg cagcaggaag aagtttgtgt gctggtcacc gatgctggtg aaattcgtct
     8941 ccgcttattt cctgaccaag ctcccctggc tgtagccaac tggcgctccc tagctgagca
     9001 aggcgtttat gacaacacac ctttcggctc gtgtcattaa ggaatttgtt attcaaggtg
     9061 gcgctttgga tggttctggt gatgaagctg tttccagcta tgagggcttt ttcgctgatg
     9121 aagtgcatcg cggactttat cattttaatg gcgccttggc cttaggggac cacggccccc
     9181 acaccaacgg caaccagttt tttattgtgc aaaacacaaa ggcgcaggct gacctgctaa
     9241 tgtaaagcaa gcatacttgg agcttggagg actaccggag ctggatggtc gttatactgt
     9301 ttttggacag gtataccaag ggttagaaat cgtagcagcg atcgcagctc agccgacaaa
     9361 cagtgaagat gtaccgattg agccagtgtt cattcgacaa attaacttcg aaaaaacatc
     9421 gtaaaaagac actcactgac ttcagcaaaa gctctattct tgagctggct gcagctagtg
     9481 agtgtctttc atttatccat aagataaagc ttggattcaa taaataaata aatgttttgg
     9541 ttaaagctgt gaccagcaaa tgaggaaccg tgcgagccag ttgatctgcc aatgcaaaca
     9601 tttctttagg tgaaggcgct ggcgtgtctg ccaacggata ctccagtccc aatgcttggt
     9661 aaccgtgcat accatagcag tggtacggta gtaattctaa cggtacgaac cgatcaagat
     9721 ctgccacaaa acaagcaatc gtcagcagct cggtcaggtt catattgata cctggaacca
     9781 caggaactcg aattcgcact tcgcagttag gatactcaat taaaaagcga cgaatattct
     9841 tcaaaatttg ttggttatct aagccggtcc attgtttgtg gagcttgctg gtaaacattt
     9901 ttacatccac aaacaatgta tccacatagg gagctacctt ttggatttca ttataagcac
     9961 cacaaaagct ggtttccagt acggtattga ttccaatgta tttcgatttt tgcaaaattt
    10021 ctttagcaaa atccgcctga accagtgcct cgcctccgct aatggtcact ccgccatccg
    10081 agtgaaagta aaaaacagca tccttctcaa tctcatccat tacctcttca gcggtcatgg
    10141 tctggccaaa tcctttttca atctgcttgc tttgtgattc gggaatcgaa caccaggcac
    10201 agcgtaatgg gcagcccttt aaataaacaa cggtccgtaa accagcacca tcatgtagag
    10261 aaccctgttc tattcttaat actgttcctt ccatatagat ttttgaatta cacactagtt
    10321 taaactaaaa cacacaagga aaaacttacc atttaatcat aaaattagct catataaata
    10381 tactaggatg taaaatagac tggtcaaaat ctggtcaaaa aagaggacta atacaggtag
    10441 tcctcttttt tgttatttta atattctaaa tcatttttta ttggtttatt cccttattcg
    10501 atgtagactc aaatcactat atcactagat atcaaacggc gtcatacgga atcaaaaaca
    10561 tcaaatttga cctgttaaat ggtcattgaa taacattcaa aatcaataaa acggagtcag
    10621 acacggagtc aaaaacgctg agagtaaaaa atatatacag ccttgtaggt aaagtaccac
    10681 agggctgtat attttttatt tcagattacc ttaaatgatt ccatttactt tttatctcat
    10741 tttttcctac tagttagtat caaatactct cctattttct caataaacta tttttggata
    10801 catggtatct gaaacagcta aaaccttgtc attacagcaa aaaatcacgt ta
//
LOCUS       DANKHJ010000087        10618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000087 DANKHJ010000000
VERSION     DANKHJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            34..657
                     /locus_tag="PW113_002523"
     CDS             34..657
                     /locus_tag="PW113_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526791.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="PW113_002524"
     CDS             1107..2156
                     /locus_tag="PW113_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2526792.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="PW113_002525"
     CDS             2153..3034
                     /locus_tag="PW113_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2526793.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="PW113_002526"
     CDS             3037..3807
                     /locus_tag="PW113_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2526794.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="PW113_002527"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="PW113_002527"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2526795.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="PW113_002528"
     CDS             5118..6281
                     /locus_tag="PW113_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2526796.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="PW113_002529"
     CDS             6398..6808
                     /locus_tag="PW113_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2526797.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="PW113_002530"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="PW113_002530"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2526798.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="PW113_002531"
     CDS             9381..9665
                     /locus_tag="PW113_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526799.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="PW113_002532"
     CDS             9825..10061
                     /locus_tag="PW113_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2526800.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="PW113_002533"
     CDS             10058..10456
                     /locus_tag="PW113_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2526801.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3712 a         1771 c         2242 g         2893 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagt
//
LOCUS       DANKHJ010000088        10353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000088 DANKHJ010000000
VERSION     DANKHJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            38..1498
                     /locus_tag="PW113_002534"
     CDS             38..1498
                     /locus_tag="PW113_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526802.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1719..3482
                     /locus_tag="PW113_002535"
     CDS             1719..3482
                     /locus_tag="PW113_002535"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2526803.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3566..4024
                     /locus_tag="PW113_002536"
     CDS             3566..4024
                     /locus_tag="PW113_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2526804.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            4133..4816
                     /locus_tag="PW113_002537"
     CDS             4133..4816
                     /locus_tag="PW113_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2526805.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4963..5577
                     /locus_tag="PW113_002538"
     CDS             4963..5577
                     /locus_tag="PW113_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2526806.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5593..5799
                     /locus_tag="PW113_002539"
     CDS             5593..5799
                     /locus_tag="PW113_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526807.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            6338..6895
                     /locus_tag="PW113_002540"
                     /pseudo
     CDS             6338..6895
                     /locus_tag="PW113_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6953..8992
                     /locus_tag="PW113_002541"
     CDS             6953..8992
                     /locus_tag="PW113_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2526808.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(9101..10252)
                     /locus_tag="PW113_002542"
     CDS             complement(9101..10252)
                     /locus_tag="PW113_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2526809.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         3733 a         1739 c         2055 g         2826 t
ORIGIN      
        1 ataaaatcat aagaaaaact ggaggcgtgt atcaggaatg ctggaaaatt acatcgagcg
       61 aaatatattt agaaaagtgt atctgtgtga acaattattt gaattccaag aaatagacat
      121 cgaacaaaca gctatttctc taagggtgac tacaccaacg atccttcacg atctagaaag
      181 tctagccgag tgtctagaat attgtataaa agaacaagta agagagaagc ataagtataa
      241 actcgtattc aaacatggta ttgctctttc tgagctcaca cagtttctgt acggtcaatc
      301 ttatttttta aagtttttaa gttattattt taatggtata ttcacgagta cagaactggc
      361 tgatcttgaa tttatttctt tgagcaaagt atatacaatc aaaaaaatcg tattggattt
      421 ttttaaagaa aatgattatt tgaaaaataa agaaatcatt atcccagaat ttgactcgcg
      481 gaacatcttg ctagctttag tacgttatgt caattgggaa ggctatgaaa ataaaaatcc
      541 gtatgtagaa gaagcttgta acgagttgat tgattatgta gagactcatt tttttaaaag
      601 aagatattcg gaagatgaaa agtcctttat catacgagga atagagatcg caatcggaag
      661 aaaggcattt ccccttggac tgaaagaaga agataagaaa agcgcagaaa cgaagccgtt
      721 atttcaatta gtctgtcaag gattggaaga gcaaaaagga attctagact tacaagaaga
      781 agatgtctat tatatttttt ctttgttcaa cagtagaaat tataccaatg agaacatgga
      841 gctgttaaga aaagatttcg aagttgttta taaaaatttt gttctcaatg acctatcact
      901 gaatgaattg attttacaga ttattagtat cgctgaaatc gacgatgcag aaaattttat
      961 tttcaagcaa tcctttttac aatttgtccg tacgttgtgg gcagatggac aagtattttt
     1021 accggaaagg atctatcttc tatcagaaaa agaacaactt ctgtacggaa aaattgtaga
     1081 aatattggaa gagtggaaaa agaaaaatca aatccaacta agatggaatg aaaatttcat
     1141 tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa caagcgggga gacaggaaat
     1201 agaaattttt gttgtttcag aaagttcagt caaacaattt ttttatcgaa cccaatttca
     1261 tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt cattagaaga
     1321 attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg ctgctagcta
     1381 tcaaaatgga ttagaaactg aaaagacaaa gatctatcca atctcattaa aaggtgcaaa
     1441 catccatttg tatcatattg ctcaggaact tcacctatta aaaaaacata tttcttgatg
     1501 agaccttaat taggagacct tctacaaagc tttttaattg tctgtagaag gtttttattt
     1561 gagtcctgat tcgtgcgaag atctacttat ataacaagct ttctctgaaa tttttttaaa
     1621 atgctattga cttagagtca actctaagta gtagaataga taaagtgtaa caaatctatt
     1681 ttcattaaaa gaagatagta cggaggagag aatgtattat gagtacagta ttacatgtat
     1741 tggatgcaat caatagcccc gcagatgtca aaaaattatc tgttgaggaa atgaaacagt
     1801 tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt gggcacgttg
     1861 gtccgaatct aggtgtagta gaattaacga ttgcgtttca ttatgtattt gattcaccaa
     1921 aggataaaat cgtttgggat gtttcccatc agtcttatgg tcataagctt ttaactggtc
     1981 gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag cggatattcc aaccaacatg
     2041 aaagtaaaca tgactatttt acagtggggc acacatctac atctatcagc ttagcaacag
     2101 gtttagctaa agcgagagat ctgaagaaag aatcaggaaa tgtggtagca tttatcggtg
     2161 acggctccct aagtggtggc ttagcttttg aaggattgaa caatggtggt gttttacaat
     2221 ccaatctaat cgtattagta aacgataatg agatgtctat tgatgagaat catggcagtt
     2281 tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat aacctattta
     2341 aagcaatggg atttgattat cgttacgtgg ataatggaaa cgatctggaa acggtgatcc
     2401 gtttatttga agaagtaaaa gatattgatc atccgattgt acttcacgtc catacggaaa
     2461 aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa atatcattgg catgtaccat
     2521 ttgatctgtc caccggggag gcaaagcaag taacttctgg agaaacattt aatatgatca
     2581 tcatggatta tttggataag aaaatgcaaa caagtacagc tcctattgcc gcagtcaatg
     2641 cagcgatacc aggcgccttt ggactgaaaa cattccaaga aaaacatcca gatcattatt
     2701 ttgacgtggg gatcgctgaa cagcactcta tcactttcgc ttctgctttg gcttctcagg
     2761 gagtacgtcc agtcgtcttc caatcaagta ctttcttgca acgggcatat gatcaattat
     2821 cacatgattt agcgatcaac gagaatccag tgattatttt agtcggtggc ggaacgatct
     2881 cagctagtga ccgaacacac caaggaacat ttgacattcc ttacttatca agtatcccga
     2941 acatcaaata tcttgctcca gcaacaaaag aggatatgat ctctatgcta gattgggcgt
     3001 tcgaacaaac agcgcagcct gtagcaatcc gaattcctac acatggtgta gaacatggta
     3061 cagctatcgc aaatgattat agtattccag cctatcaaac agtaaataat ggaggaaaag
     3121 tagcaatttt agctttaggc ggttttcttg aattaggaaa agaaacagta aaagctttta
     3181 gaaaaaaagg aatcgaagca acgttgatca atccaggaac gatcacagaa aatgacgtag
     3241 aaacattaga taaattaaaa gaaacacacg aattagtcat cacattagaa gatggtagtt
     3301 tgtcaggagg atttggcgaa aatatcagtc gttattatgg tgattcttca atgaacgtat
     3361 taaattttgg tgcagagaaa gaattcacgg atagcgttcc tgtagggcag ctttatgaac
     3421 gttatcattt aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg aatattcaat
     3481 aattcattat gctcaccatc cagataattg tttatactag tatctggatg gttttttcat
     3541 tataaaaagc aaaggggtgg atgttatgta tcagcaagta gaagaatatg tcaaaaagtt
     3601 ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt ttcttttcat ttggggatac
     3661 agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa aagataaaga ctggtacatc
     3721 aagtggttac gatctgtata aacaagagaa tgagccttta ccaaaagttg gtcaattaga
     3781 cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa atgtgaccat
     3841 cttgcctttt agtgaagtca cgaaagagca tgcttggaaa gaaggagaag gggatcggac
     3901 attagcttat tggcggaaag tccatgctga gttttttaaa catgcttata aagaagcgtt
     3961 gaacatagaa tttacagaag agaagctggt cgtatacgaa gaatttgaag taattttctt
     4021 ttagtaagaa aggtctgctg aaagaaatag ctcattattg tctcctgaat agaaaagggt
     4081 tacattttta tatgaagctg tatttttata taacattact ggaggagata ttgtggatac
     4141 actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa acgagccgct
     4201 acacagttct tttattatgg ggagaatggc tttggctgca aaagaaggcg gagcagttgg
     4261 tattcgagca aatacgacag aagatatcaa tgaaatcaag cgaatcgttg atttaccggt
     4321 aatagggatt atcaaaagag attacgatgg gtcagaagtg tttattactg cgacagaaaa
     4381 agaagtggaa gaacttttgg agacgcaagc agaaatcatt gcactggatg caacttctcg
     4441 taaacgtccg acagaaaaaa caactgctga acttgtccaa atgattcacg aacacggtcg
     4501 gttagctatg gcagatattt cgacttttga ggaagcgctt caagcgcaag aagatggttt
     4561 tgatcttttg tctacgactt tagcaggcta tacagaatat tcacgaaaga cgcagacacc
     4621 tgattttgat ttattgaatc aaatcataga acaagtcgat atcccagtaa ttatggaagg
     4681 acatactgac tctcctgaac aagtagagaa agcatatgaa ttaggtgctt tttcagtcgt
     4741 agttggatct attatcaccc gtccgcaatt gatcactaaa agatatgtcg aagccgcaag
     4801 taaagcaaat aactaaaaaa cacgcacggc acagattctt gacaaaaaag aaaaattgat
     4861 agatagttta aagcagattc taagaagatg aaaaaagcat tacttttcat tttgtcttca
     4921 agatctgctt tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga acggagtcct
     4981 tcagaattga cagaacgtga aaattacaag ttgctgattg gttcagtcat ccctagacca
     5041 gtagcagtag taacgactaa atcagcaaaa gatatagtga atatcgcacc cttcagttac
     5101 ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg cgatccagag aaaacaagat
     5161 gcagtaaaag acacagcaaa gaatattctt gagacaagag aagctgttat ccatatcctt
     5221 gatgaggaca acgtagaaga agtaaataaa acagcagcca atctgcctaa tgaggaaagc
     5281 gaactgtacc tgactgactt gactttaact gattcagtaa tcatttcagt accagcattg
     5341 acagaagcaa aagttcgttt tgaaacatcc ttatatgaac atgtcgagat acggaataaa
     5401 aataaagtaa cagctgattt atttttgcta gaagtacaga aatatcattt aaatgaaaca
     5461 gtctatgatg ataagacagg aaggatcgat ccaagaaaat tgaatgctgt aagccgtttg
     5521 gcaggaaatg attatgcagc aataggagaa attttcacga ttgcacgtcc cgaataaaaa
     5581 ggagcactag ttatgttaaa aataagctat aaaccatcga cagattcaaa agaaatgaaa
     5641 aaagaatatg aaacagtgaa tgattttctg caagggcagt acttagaagt tccgccattg
     5701 caagaccatt ttgtcgttac tactgttact ttagatggta aggaaataga aatgccagat
     5761 caaacgatta gcggattgtt taattacttt aacaaataaa tatttattcc gaaaagtgaa
     5821 aaaacatata aaaaaacaca taaaactaaa aaattatcaa agaatcctgg tgcaactgaa
     5881 agaatttgca acgggattct ttcaacttat ttgttatttt tttctaaaaa atattaaaga
     5941 ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga aaagtctatc
     6001 atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta tacaaacata
     6061 ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg ttggattaag
     6121 gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa ctcggacgat
     6181 gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc tttatatttt
     6241 tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg attgctgtgt
     6301 gaggaggaga tgcggtgaaa aaagatcgat tggtggaggt cgctcggatt cacttctggt
     6361 tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga gagattcata
     6421 tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt atgggggagt
     6481 tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct attatgcaag
     6541 tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg gagtcaagat
     6601 cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag agcaagtgat
     6661 ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag gagacttcgg
     6721 gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga aagacgtaac
     6781 ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc aaacgaacct
     6841 tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat gttagaataa
     6901 acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa aaatgaaaag
     6961 aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag tgatcttaat
     7021 agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc aagcagtaga
     7081 agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag attataacaa
     7141 agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg aaaaagagat
     7201 cttagatcaa taccagaata tatacggtgc tgctgatgtc aaaggacttc agatatcgaa
     7261 tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag caaagatgaa
     7321 tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca gaaatgatgg
     7381 tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag gaaatgacaa
     7441 agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa atggtgaacc
     7501 attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac ttggagatgg
     7561 aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa cagaagatgc
     7621 tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc cattgaaaat
     7681 cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag tagacggcag
     7741 atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag atatcacagc
     7801 agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc gctcaggttt
     7861 ggagatggct tttgataagg atcttcgtgg gacaacaggt gagaaattaa gcattacaga
     7921 tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg gcaaagatat
     7981 caaattgaca atcgatgcga aggcacaaaa aacagctttt gacagtctag gaggaaaagc
     8041 tggatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg ctagctctcc
     8101 aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca aagcttatga
     8161 agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc ctggatctac
     8221 gtttaaaata attacagcag caatcggtct cgacaacggc actatcgatc caaatgaagt
     8281 gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt ttaaaacaac
     8341 gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct attccgataa
     8401 tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta caggcttaga
     8461 taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag cacaaatttc
     8521 taatgaagat agctttaact cagatatctt gctagctgat actggatatg gacagggcga
     8581 acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca acaatggcac
     8641 acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga atgtcatcag
     8701 cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag atgtaaatgg
     8761 tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg gtacagcgga
     8821 aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg ctttcaatct
     8881 tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagatgatg atgattcagc
     8941 aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat aaaaaattgc
     9001 tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt caaataaacg
     9061 gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca taaacttttt
     9121 ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc acgataacta
     9181 aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt aaaatgatca
     9241 aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta tgttgagaaa
     9301 aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc gctacaagcg
     9361 tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact tctaatccta
     9421 cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca ttgtggtata
     9481 cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca ccaatgacag
     9541 agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact agcgatacga
     9601 tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa ggcgcaatcc
     9661 atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa acaagcggta
     9721 aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt agaactaaaa
     9781 cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct tttgtattca
     9841 ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc gcaagaataa
     9901 tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc aaccagccaa
     9961 aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg aaatattttt
    10021 tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagaac acaccaatct
    10081 ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct tggcctagca
    10141 aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc gagatatgtg
    10201 agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc attctcatca
    10261 ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa aaaataggaa
    10321 aatatactca caaaaaaacc aacaatccaa aag
//
LOCUS       DANKHJ010000089        10137 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000089 DANKHJ010000000
VERSION     DANKHJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            158..730
                     /locus_tag="PW113_002543"
     CDS             158..730
                     /locus_tag="PW113_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2526810.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="PW113_002544"
     CDS             875..1579
                     /locus_tag="PW113_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2526811.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="PW113_002545"
     CDS             1807..2145
                     /locus_tag="PW113_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526812.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /locus_tag="PW113_002546"
     CDS             2130..2984
                     /locus_tag="PW113_002546"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2526813.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="PW113_002547"
     CDS             3131..3364
                     /locus_tag="PW113_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2526814.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="PW113_002548"
     CDS             3562..5466
                     /locus_tag="PW113_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2526815.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="PW113_002549"
     CDS             5776..6138
                     /locus_tag="PW113_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526816.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="PW113_002550"
     CDS             6155..6766
                     /locus_tag="PW113_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDL2526817.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="PW113_002551"
     CDS             complement(6868..7776)
                     /locus_tag="PW113_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2526818.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="PW113_002552"
     CDS             complement(7818..8132)
                     /locus_tag="PW113_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2526819.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..>10137
                     /locus_tag="PW113_002553"
     CDS             8431..>10137
                     /locus_tag="PW113_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2526820.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDL"
BASE COUNT         3288 a         1680 c         2165 g         3004 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgattta
//
LOCUS       DANKHJ010000090        10020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000090 DANKHJ010000000
VERSION     DANKHJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(25..162)
                     /locus_tag="PW113_002554"
     CDS             complement(25..162)
                     /locus_tag="PW113_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2526821.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="PW113_002555"
     CDS             complement(159..1343)
                     /locus_tag="PW113_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2526822.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="PW113_002556"
     CDS             complement(1599..1853)
                     /locus_tag="PW113_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526823.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="PW113_002557"
     CDS             complement(1878..3020)
                     /locus_tag="PW113_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2526824.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="PW113_002558"
     CDS             complement(3080..4432)
                     /locus_tag="PW113_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2526825.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="PW113_002559"
     CDS             complement(4503..4847)
                     /locus_tag="PW113_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2526826.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="PW113_002560"
     CDS             complement(4857..5231)
                     /locus_tag="PW113_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2526827.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="PW113_002561"
     CDS             complement(5244..5558)
                     /locus_tag="PW113_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2526828.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="PW113_002562"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="PW113_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2526829.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..9640)
                     /locus_tag="PW113_002563"
     CDS             complement(8978..9640)
                     /locus_tag="PW113_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526830.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2702 a         2262 c         1710 g         3346 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcgac atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgattttt ccaaatgata cgtccatcat tttgcttcac gataaaaact cctaatagtt
     9661 atttagatag aagcctatca catcaacttt ttagtagaaa agtacatgtt ttgtcttttt
     9721 tgctaatagc tgaccctttg agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt
     9781 ttggtatttt tcgtattata tttgtcgtca agctgactac taaattttgc cctattttga
     9841 cgacactgat agtcaatgtc ggacaatcta ggacaatttt taagggaata tgattgaaaa
     9901 atggtccaaa acagccaaaa atggcttaaa actgtgatag agaaaaaaga aaaagccctt
     9961 gatatacaag gacttttcac aaatttcaga tttccaagga agttttccgt gtttatcttc
//
LOCUS       DANKHJ010000091        10001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000091 DANKHJ010000000
VERSION     DANKHJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1149
                     /locus_tag="PW113_002564"
     CDS             <1..1149
                     /locus_tag="PW113_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL2526831.1"
                     /translation="PYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAF
                     IGMLASILVLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVT
                     IFKLVDQFTFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLI
                     TEAVTLKDRVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQ
                     ASFVGLFLGLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLAT
                     MIGFAVSCTLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNED
                     SKFQSLLLILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            1198..1917
                     /locus_tag="PW113_002565"
     CDS             1198..1917
                     /locus_tag="PW113_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2526832.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            2396..3343
                     /locus_tag="PW113_002566"
     CDS             2396..3343
                     /locus_tag="PW113_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2526833.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            3355..4275
                     /locus_tag="PW113_002567"
     CDS             3355..4275
                     /locus_tag="PW113_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2526834.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            4316..5773
                     /locus_tag="PW113_002568"
     CDS             4316..5773
                     /locus_tag="PW113_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2526835.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            6118..6732
                     /locus_tag="PW113_002569"
     CDS             6118..6732
                     /locus_tag="PW113_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2526836.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            6725..8449
                     /locus_tag="PW113_002570"
     CDS             6725..8449
                     /locus_tag="PW113_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2526837.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            8446..9897
                     /locus_tag="PW113_002571"
     CDS             8446..9897
                     /locus_tag="PW113_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526838.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         3305 a         1675 c         2030 g         2991 t
ORIGIN      
        1 ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct
       61 acattcatca tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact
      121 ttcgcggcat ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa
      181 catcaagcgt atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg
      241 aaggaattgt tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg
      301 gtaacgatct tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc
      361 gagtattcca atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag
      421 ttgacgatgg tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt
      481 acagaagctg ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta
      541 cagttgtttt cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg
      601 ctaaatacat tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt
      661 tttgtaggac tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt
      721 gaaaacaagg cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa
      781 tacccagcca ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt
      841 gctgtgtctt gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt
      901 tttgtctggc gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg
      961 tggatcacaa agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta
     1021 ttgttgattc ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa
     1081 atccgcttgg cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa
     1141 ataaaataag tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg
     1201 cgtttagata agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggattg
     1261 atcaaaaaag gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta
     1321 aatgaattcg aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt
     1381 tactacattt tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa
     1441 accgtaatcg atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga
     1501 ttagataaag acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag
     1561 ttactatcac ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg
     1621 acagaagaag atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca
     1681 ttgccggcaa aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt
     1741 ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa
     1801 gaagtcttat atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat
     1861 aaagggcaat atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg
     1921 aatggatagc taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc
     1981 cgtctgactt tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc
     2041 tggaacaccg tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg
     2101 ttccttgtga ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac
     2161 gctgaattca caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa
     2221 aaaacggcaa aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac
     2281 gctgatttca caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt
     2341 ctcattattg aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa
     2401 aaaacgaaag gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct
     2461 gatggctgta ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa
     2521 tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga
     2581 aagtccttgg gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct
     2641 catcactaaa aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc
     2701 agtcgtattt gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca
     2761 aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc
     2821 ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc
     2881 tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg
     2941 gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac
     3001 ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac
     3061 gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt
     3121 ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt
     3181 gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat
     3241 gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa
     3301 tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa
     3361 aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct
     3421 aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc
     3481 atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag
     3541 ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg
     3601 cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct
     3661 ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt
     3721 tgtttaatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat
     3781 cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc
     3841 aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg
     3901 gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct
     3961 gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc
     4021 aatgctttac tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa
     4081 atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc
     4141 ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg
     4201 cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc
     4261 ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa
     4321 gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc
     4381 atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat
     4441 gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg
     4501 aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga
     4561 aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa
     4621 ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta
     4681 cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg
     4741 aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa
     4801 caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc
     4861 agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg
     4921 acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc
     4981 tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat
     5041 cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc
     5101 aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca
     5161 aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc
     5221 aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt
     5281 atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga
     5341 tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga
     5401 tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc
     5461 ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat
     5521 tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt
     5581 caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt
     5641 agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc
     5701 tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt
     5761 ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct
     5821 cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta
     5881 aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt
     5941 cacaaactct tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga
     6001 caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt
     6061 gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg
     6121 ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg
     6181 tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa
     6241 acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga
     6301 ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta
     6361 tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt
     6421 tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa
     6481 tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt
     6541 tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata
     6601 attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg
     6661 acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa
     6721 agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg
     6781 gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca
     6841 tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac
     6901 aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg
     6961 gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct
     7021 gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat
     7081 caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact
     7141 accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc
     7201 aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg
     7261 aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc
     7321 attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca
     7381 tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct
     7441 gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca
     7501 agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga
     7561 aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct
     7621 tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc
     7681 catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata
     7741 tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt
     7801 agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc
     7861 acagatcaat ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag
     7921 cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa
     7981 tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt
     8041 ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga
     8101 tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa
     8161 acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg
     8221 caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa
     8281 acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca
     8341 aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa
     8401 taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa
     8461 gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg
     8521 caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta
     8581 cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga aataaatgga
     8641 ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca
     8701 ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg
     8761 atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta
     8821 gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa
     8881 tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag
     8941 aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga
     9001 tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg
     9061 atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac
     9121 tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc
     9181 cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa
     9241 gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca
     9301 agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat
     9361 ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt
     9421 ttactgttta tggagattca acgggaattg attgatctgg aagatgagga atatttacaa
     9481 ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca
     9541 ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta
     9601 atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat
     9661 ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct
     9721 tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac
     9781 atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc
     9841 aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaaa
     9901 aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg
     9961 ttcttattac ttgaggcaag gcagcttttt catgacctct c
//
LOCUS       DANKHJ010000092         9986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000092 DANKHJ010000000
VERSION     DANKHJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(341..661)
                     /locus_tag="PW113_002572"
     CDS             complement(341..661)
                     /locus_tag="PW113_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526839.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1164..2282)
                     /locus_tag="PW113_002573"
     CDS             complement(1164..2282)
                     /locus_tag="PW113_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526840.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2753..4060
                     /locus_tag="PW113_002574"
     CDS             2753..4060
                     /locus_tag="PW113_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2526841.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4181..5380
                     /locus_tag="PW113_002575"
     CDS             4181..5380
                     /locus_tag="PW113_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2526842.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5407..6675)
                     /locus_tag="PW113_002576"
     CDS             complement(5407..6675)
                     /locus_tag="PW113_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2526843.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(6845..7486)
                     /locus_tag="PW113_002577"
     CDS             complement(6845..7486)
                     /locus_tag="PW113_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2526844.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(7895..8398)
                     /locus_tag="PW113_002578"
     CDS             complement(7895..8398)
                     /locus_tag="PW113_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2526845.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8521..9285)
                     /locus_tag="PW113_002579"
     CDS             complement(8521..9285)
                     /locus_tag="PW113_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2526846.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9393..9668
                     /locus_tag="PW113_002580"
     CDS             9393..9668
                     /locus_tag="PW113_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2526847.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         3016 a         2095 c         1765 g         3110 t
ORIGIN      
        1 cctatagtat atcataccgt ttacaaaaaa gctagagttt ttcgactttt tttattaaaa
       61 aaagttttct cagcgattac ataaagtgaa aaaccgaact tttttttaaa ataaacgaca
      121 tattgttcag ttggtgtttt tttgctctga tgttcttttt taaactaaaa aaattattct
      181 ttctgactac ttatcggttc tgtttcaacg gtcgaggctg gggaagattc tttagtaggt
      241 ctgatgttct catggtattt ctgatagcct atgacagtta ccagaagaac aaggcaaacc
      301 aatacgacca acgagattag tttttctttt ttgctcatat ctatacctca taaataacag
      361 gatgatgtcc atcatatttc tgagtatgag taaaagcaaa agctgcagct gtagaatgta
      421 agaaatgcca gcaactttca taatcacagg tcggacagta ccaaaagtaa ccaaatcttg
      481 gctgcaaaga ccgttcttct gtttcaggaa tcgcagaatc aagatctggt gtctgtacat
      541 aaacattttt gtacagacgt gtgcctgtaa taggaaaccg ctgtgtttct tcagcaaaaa
      601 ctggctgact tgttaaactt agggatagag acagcaaaca ccataacagt ctttttttca
      661 taaagacctc cttatcgata agtttttccc aattaaaatc atatctcaag aacgcccgtt
      721 attcacattt tgttcataaa tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga
      781 ccacgtcaca acctcttttt taaacggtgt tccagaagta gcttcttcgg aaataagccg
      841 aaatttcaca aaaatttgag aaataatttt tgtcaatctc ttcttatttc ttgaagctga
      901 ccacttctgt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
      961 gcccaccaaa caaaaaatat gaagagctat ttttgcttgc tgttcttatt acttgatgca
     1021 gaacagcttt ttcacaacct ctcatacacg gtgtttaatc agctgacctt cggtattagc
     1081 tcaaaaaacg gcaaaatatg tgaaactatt tttccgtttg tttctctaat actcaggtca
     1141 aaacactgat ttcacaacct cttttaataa cgaagaatcg ctgtcagact cttttcatct
     1201 ggcgcatctt tttgatccaa aataaacact tttccgccat taatgatcac atcatctgct
     1261 aatgcattca acacttgacg ccgatcatat tctgtttcag gatcttcacc gaaaccatca
     1321 gctaagtttg acgtagcgat aaataaatgt gacacttttc cttctttcgc agctggaacg
     1381 atatcaacta actgatcgat gaatttctta tctaagagtt tattgtattc ttttgattct
     1441 tgagctgcga gttcacttga cagcttcttc gccccttctt cgatatctct aaaggatagc
     1501 tgcgcaggag aagcagaaat cgcaacgtct gcacaataat agggattttt agcaattttt
     1561 ttgaacatcg tctgattttc tggtagagca aataaataaa tcggccattg ttctggattt
     1621 tcgaaagaat ccttcaagta cgtatcaact gcttggtaat agttgaccca atcgatctct
     1681 acttcttcat cttttggatt tacgccatgg aaagttactc catctcgggc tccgcctcct
     1741 ccattagaag agaagttcaa accgccacct gtcagttcag atcccaatgc agtaacgaca
     1801 tcaaccggtg ccccttctgg tagagatact tcttttactt gtttgttgct gacttcatac
     1861 aatttcatgg agtcgcggtt caatcccaat aaataataag aatagttgaa ctgcccattt
     1921 ttgataatag ccagtaaata aggaagatca cttacatagt attgatcgtc taccgggata
     1981 ctcaaacgat ggatataggt ttggtcagca gttataatga ccgctacact cgtagttgca
     2041 ctacgccaaa acgaaggatc tcctaacaaa ttttcaattt tttcttggaa cggggaccag
     2101 tcatgttcgg aatatttttt ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat
     2161 ttgatctgat ctttttccac atcttgatgg gcgacatgcg tattcagcat gatcgtgacg
     2221 aatggtcctt tagcgccttc tgaagtcaat tgtgacaata attctttatc cttttttgcc
     2281 ataaacgtac ccctcctaaa aaaatcattc ctttgtttct atctaaagtc taccacaaca
     2341 ctatttttcc atacaataaa acgtatctaa aaaaataaaa cggtttctac ggttttaaat
     2401 ttagctattt ttttaagtta tcttcataat ttcctcaatt atttagcccc agtcttcaac
     2461 tattcttcat gaaatgctga taaactaaga aaaagaataa aagaggtgga tcttatgcat
     2521 ttgattatga ttttcagttt attaagtatt atcagtttat ttgctttcag cgcttcccgc
     2581 ttattagatg cggaaacaca gcctattcct gtaaaagtac gtaaaaacga ccgctaacct
     2641 tccataacgt gcgttcttat cctaaagttt tttcagaaac attgaaactc tctcccctct
     2701 ctgataaaat tttaatttgt agcgaaacaa attaattaca aggagatcgg atatgtccat
     2761 aaaaaaaaca cgtctagtcc tattattggc tagtctcatt ctttgccttg cttcttttct
     2821 accagctgtt gctgtgcaag ctgaagatac tttcaaagta aatgcaaaag cagcctttgc
     2881 tgtagatgcg caaagcggta agattcttta tgatcaagat ggagaaaaaa ccatggggat
     2941 cgcttcaatc acaaaaatca tcggtcttta catcgtcctt gatcaagtaa aagaagggaa
     3001 actatcatgg gatgataaag tttcaatttc agattatgca gaaaatttga gtgtcacccc
     3061 agatttatca aatgttcctt tgcataaaga aaacacctac accgttaaag aattattcga
     3121 ctctgcaatc atccagtcag ccaacgcttc aatggttgcc ttggcagaaa aaatctctgg
     3181 cagcgaagca aaattcactg aacgaatgaa agaacaattg aaagattggg gtatcaaaga
     3241 tgcaacgatc gttaatgcat caggactgaa taactcttat cttggcgaaa accgaccaga
     3301 aggtactgga gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcta tcgttgcccg
     3361 tcatttgatc ttggattttc cagaaatact ggatgtatct agcactacga cacaaatgtt
     3421 tggcgaaaac acgcaatcac cagtagaaat ggtcaactgg aactggatgc tcccaggatt
     3481 cgtgaattat aaagaaggcg tggacggatt aaaaactggt acgactgaac ttgcaggagc
     3541 ctgtttcgtc ggtacgatca ctaaaaacga tcaacgaatc atcactgtcg tattgaatgc
     3601 agacggccat gcggaaaacc caagtgcccg tttcaatgaa acagctcgct taatggatta
     3661 ttgctacgac aattggtcag aaaaagagct tggaaaagcc aatgcaagta tccctagtct
     3721 aaaaaccatt gatgtaaaag acggaaaaga atcaacagtg cccgttgtgc ttaaaagtcc
     3781 tgtaaaagta tgggtacgga acgatatgga tactggaaaa ttaacaatca caccaactct
     3841 tgacaaaaaa caagtcactg acaatgagat ccaagcacct atggataaag gaacaaaaat
     3901 cggtaccgca acgatcacct tggcagaaga taaattaggt tatcttgaag acaacaacgc
     3961 gccatccaca gaaatcatca cggataaaac cgtagaaaaa gcaaatattt tcgttattgg
     4021 ctggcgacgc gtcgcagaat ttttcggtaa cttgttttaa ttgaacaaaa agaaaaaagc
     4081 atctttccat gtggaagatg tttttttgct ttttctagtt taaataacag cttttttcat
     4141 ctattttcgt tatactgtat aagaaaaagg agttgaattt atgaatctaa ccagagatct
     4201 gatttttttg gttctctatc ttattggggt attctccatc gtttattggc gaaaacagaa
     4261 aaaggatcct tccttgttct tttacagcag tacatggacg tttgcggtaa tcttgactta
     4321 ttttgtcgtg ttggaaattt tttattcgac cttttatttt ttgattccct tagtgttttt
     4381 cagtatcttt gccttttcct atttttcaga aaagcgaagg ctgttgaatg gtcttttatt
     4441 caatattttc ctcattagtt ttggtatcta cttgtttgtc ttgctatacg aaacacaaaa
     4501 tatttttctt ggcggactga ttgccttgat cacgatcccg ttactgcttg tctttctatt
     4561 cggcatttat ggattgatcg tttttctttt ttggaacggt gtcacagttt tacgtagaga
     4621 gtcgcattca ttagctaatc ttttaacgtt gatactagcc atttttctta cattgtttct
     4681 tgtttttgac tttttcttgt taaaatattt accacaatgg ataaatgctt tgtttttctg
     4741 catcccattg atcttgatct atctatttat cgtattctac aactttttga ctgtctcatt
     4801 cttgtatcag ttcaaccgcc ctcgatacaa ccaagacttc atcgttgtgt taggcgccgg
     4861 gttgatcaat ggagaaacag tttctcctct tcttgccaag cgaatcaata aggcgattgc
     4921 tttttattgg gcacaaagcc gtgcgacatt aaatccgcct atcctattaa tgtcgggtgg
     4981 acagggagca gacgaaaaag taccagaagc aatggcgatg aaacaatatg ccatggaaca
     5041 aggaattcct gaaagagata ttcttgtcga aaccaattcg acaaccactt tggagaatat
     5101 gcgctattca aaggaaatca tggatcagca aatgaaaggt ccctatcgcg cgatcttcag
     5161 ttccaacaat taccacattt tcagagctgg tctctatgca cgacaagcga aactgaaggc
     5221 aaatggcatt ggggcaaaaa cagctttcta ttacttgcct aacgcgtttt taagagaata
     5281 catcgccatc ttgatgatga acaaaaaaag acatatgatc atttgcggat tgcttttcgc
     5341 aggttccgca ttcttatcac tgatcacttt acttttctaa aaaaacaagg tgtgacaaaa
     5401 catatgtcac accttgtttt tttcttttat tctcttgacg attggcaaga atagtattcc
     5461 ttttttgacg gctacttgct gtgcagaata aataccgctg tttcctgaac acataaagct
     5521 gatcaagcag atcataaaga agtaagcagc agcttgtgaa ccaaataatt cgattcccat
     5581 cacaaaacag gcaatcggtg tatttgtcgc accagaaaat accccgataa aacctaatcc
     5641 agcaagaaaa ggaacagaaa catggagcaa aggcgctagt actgcgccaa gcgttgcacc
     5701 aatctcaaat agtggtgtca cttctccccc ttggtacccc gcacctaaag acaagaccgt
     5761 gaagaataat ttcccaataa aatcataagc atgtgcctgt ccattaaatg attcctgcag
     5821 taacggaaga ctcaacccaa ggtaacgttg tgtttgcaaa atcaacgctg cggctacaac
     5881 tactgctcca cctatcgtat ttcgccaaac aggatgagga atccattgcg cataccgcgc
     5941 ttttaaaaag acgatcgaac ggctgaatac ccaaccaatc aaaccaaaag caatcccagc
     6001 aacaaaaact ttgaagaaca gcaacatact ccattcagga atcacaccca tttggtaatg
     6061 tagatgagaa acaccatagc tttctgtcat gaaatttgca aaaagcgcag caaagaagct
     6121 aggaaatatt gcttctgttc ggactttacc gattgccaac acttctaatc caaataatgt
     6181 tccagctaaa ggtgtgccaa atacagaact aaaacccgca ctgattccac tgataaccaa
     6241 aatctcccgt tctgctttat ccaatcggaa taaatgcgct atattatcag ctaatgctcc
     6301 ccccatttga actgctgttc cttcacgacc aacagatccc ccaaataaat gggtagtgat
     6361 ggtcccaaac aaggtcaatg gaatgagccg caaagggatc ttctcttctc ctccattccc
     6421 ttgatcgatc actaagttat tcccacgact agaaagaccg ccatgatacg cataaagata
     6481 agcaaaaagc gcgccgctga ctggcaatag ataaagtagc caaggatgtg ctagtcggat
     6541 atctgtcacg aacgtcaggc tatttaagaa aaaagcagac aagctcccca tgaatactcc
     6601 gagaatcgct gtgaccacca gccacttcaa catgtacatc aaaatacgca ctggactcgg
     6661 taattgtttt tccataaaaa attcctcctc aaataaaaaa acgcctacta aagaaagggg
     6721 ctggccccag actttagtag gcgtcattag ctatcttacg ttagcattta aaggcgaaca
     6781 ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa caataaaatt
     6841 atcgttattt ctttacacgt tcagcaaatt catagaaagg agccaatttc gttaactgtt
     6901 gattacattg atcactatca tgacaaatat aattcccctt tttcgtatac gtgccatcag
     6961 aaccggcttt cgttgtagaa agaaagagcg agacattcga tgttttctga cagatcgcac
     7021 agattccctt attaatcgtt ggtgataaag taccgctgat cccttttaat tttccatctt
     7081 cgtaataaac catgaatttt ttttgcgtcc ctggatcatt ccaaccgatg tacgtatgtg
     7141 cttgcaagtc gatttcattc caagcgggtt gtttgagttt tttcactttt cggaataatt
     7201 tctcgatttg tggtttgctc ggctgcataa acggctggac gatttgcttc agctcctcca
     7261 atgctcgttc tgcttgcgtt tttgtcagtt ttacatctaa aactgtcgtt aaaaacgtat
     7321 cgatttctga tggttcgtaa cctaatacat cgcgaatctt ttcagctgct agagcctgaa
     7381 ccgtttgtac cgtttttgag tcattgacag atgagtacgc atttaataac tgctctactt
     7441 ctctttttat aaaaaaatat tgatatggtt tcattgtttg ttccatgatt ttaaagtcct
     7501 ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta
     7561 cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat cacccccttt
     7621 aatgttcatt cttctatgta catgattcat tcgataaata aaagtaaacc gtatcttgag
     7681 cagatagtca actgcaaaca gaaaaaggac tgtgacaaaa gtcgtgtcaa agtccctttc
     7741 tacaaacaaa cggtgttcca gaagtagctt cttcaaaaaa taagccggaa tttcacaaaa
     7801 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgaccc acttctgtca
     7861 caacctcaat atggtctatt attggtttta cctgttaaat cgaggattct tttccgaagt
     7921 aacgttgcca tctcttacgc atacttgctg acataggctg agcggcttct ttgactgcgc
     7981 agtatttgtc tacgaatgtt acgatatatc cttctttgta cttaggcggt gcaatcgttg
     8041 cgccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatctttt
     8101 ctgcattctt tacagcgatt cttggatgga tatacgcatg tgtgccttca tcaaatttcg
     8161 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
     8221 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
     8281 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
     8341 ctaaaagatc ttctacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
     8401 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
     8461 cagtgttcgg attattctag tcgccttgga taaaatagcc aaagcttaat agttccttaa
     8521 tcataaagaa aaatgataaa gaacaatccc tgccgcaacc ccaacattca acgattccgc
     8581 atttcctttc atcgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
     8641 cccctgacct tcgttcccca tgattattgc gaagttatct gatttttcta ctttgtctaa
     8701 ggcgtgtgct tcttcattta attctgtgcc atacactgta aaccctttgt ctttcattaa
     8761 aggaatcact tctgtcaaat ctttttgcaa cacaggcaaa tgataattgc ttccctgcat
     8821 gctgcgcagc acctttgtac tataaatatc tgccgttcct ttgcctaata aaacaccagc
     8881 catatcaaat gcatctgccg tacggatcat cgtccctaca tttccaggat cttgtacatt
     8941 atctaataac agccatcctc ctgaaaaatc tggcgtgcct tcagccgtta acgccatcac
     9001 tgctaagatt ccttgtggtg taggtagatc cgatacagca tccatgacct catccgaaac
     9061 tagaaaagta gaaatctctt tgtttgcggc cagccagtct ccccattctt tcatgcctcg
     9121 ctgtgtcaca aaaagttctt ttatttctgc atcagacgac gcagcttctt caatcagatg
     9181 gaacccttcc aaaagatatt cttccttttc ttcccgatat ttctttttat gtagtttttt
     9241 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcccc
     9301 atgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     9361 aatttataaa ataggaaaga aaggtgacga acatgcgcaa agttaaaatg aacgtccaag
     9421 gacgcgtcca aggcgtagga tttcgctaca tgaccaagat ggtcgctgat caactaggcg
     9481 tgactggaac ggtaaaaaac gaagatgacg gctcagtcac gatcgaagca atcggcaatg
     9541 acgacatcat ccagaaattt attgaagaag tcaagaaatc tccgagccca agtggacgag
     9601 tgcagtatgt ggatgtccaa gaagacccga tgattgagga acgaaagaaa tttgatgtgg
     9661 tgggataagg ttgtgaaaaa gctggtctgc atcaagcatt aaggacaggt tgtgaaaaat
     9721 agctctccat atttttcaac aggctggact taatgccgta gatgcaagct tttgaacacc
     9781 gtcaataagg ttgtgagaaa gtcgcttagt tttgagtagt aaggagaatt ttgtgaaaat
     9841 ggctttccac atttttgcat aaaatttgac ttaataccga gaaactgact tttgaacacc
     9901 gtcgataagg ttgtttttga gacgctttgc ctcaagtatt aaagataggt agtaaaaaag
     9961 ctgatctata acttgcaata aatttg
//
LOCUS       DANKHJ010000093         9948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000093 DANKHJ010000000
VERSION     DANKHJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            265..957
                     /locus_tag="PW113_002581"
     CDS             265..957
                     /locus_tag="PW113_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2526848.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
     gene            1385..1777
                     /locus_tag="PW113_002582"
     CDS             1385..1777
                     /locus_tag="PW113_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526849.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(1950..2132)
                     /locus_tag="PW113_002583"
                     /pseudo
     CDS             complement(1950..2132)
                     /locus_tag="PW113_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            2119..2316
                     /locus_tag="PW113_002584"
     CDS             2119..2316
                     /locus_tag="PW113_002584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526850.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(2359..2934)
                     /locus_tag="PW113_002585"
     CDS             complement(2359..2934)
                     /locus_tag="PW113_002585"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2526851.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(3089..3615)
                     /locus_tag="PW113_002586"
                     /pseudo
     CDS             complement(3089..3615)
                     /locus_tag="PW113_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4072..5583
                     /gene="gadC"
                     /locus_tag="PW113_002587"
     CDS             4072..5583
                     /gene="gadC"
                     /locus_tag="PW113_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL2526852.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            5596..6996
                     /locus_tag="PW113_002588"
     CDS             5596..6996
                     /locus_tag="PW113_002588"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL2526853.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(7161..8596)
                     /locus_tag="PW113_002589"
                     /pseudo
     CDS             complement(7161..8596)
                     /locus_tag="PW113_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            8989..9582
                     /locus_tag="PW113_002590"
     CDS             8989..9582
                     /locus_tag="PW113_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDL2526854.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
     gene            complement(9566..>9948)
                     /locus_tag="PW113_002591"
     CDS             complement(9566..>9948)
                     /locus_tag="PW113_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2526855.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         3310 a         1589 c         1749 g         3300 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc ttttaagtaa aaaatatagg tgattttttt tgacttcata tttataataa
     1321 agacacgtaa atcaattatt tttcatttta ctttaactaa taatgtttat ttggagggat
     1381 tattatgtta gatgatagat tgacgtgttc taattgtggt acattatttt actatgagga
     1441 agttagaaat attgtaaaac atagaaaaaa agaaatgcct atacgttgtc caaattgcaa
     1501 tgaagttatt aagcgaaagt tgtctcatgg ttattttgtc agctacgaac tatatgaaga
     1561 aagtaaaata aatctgaaaa aaaaaccaaa atactataaa aatttgaaaa ctggagctat
     1621 tataactgaa aaagaattta aaaatttagt gactgaagaa gaaagcaggt tttataattt
     1681 gtctggtaat atttgccctg gatttatgga tcagattatt gaaaatgagt caattgaatc
     1741 aattgaaaac tatgttcctg ttgacttacc aaaatagaaa aaataatcct gtttttacag
     1801 tgaattattt taattttgat tttcaataaa aatatattac aataataatc gtaatagcta
     1861 atgaaacaat ttctcaatct ttttaccact ccaaacaggg agcgtcaata attttgtgta
     1921 aataactcgt ccttctgcaa aataatgggt tactcagtaa acattgaagc taatgtatcc
     1981 gtcacttgtt ggaagccttt atggcttcta tttaagaatt tttgattgta tgtatcaaag
     2041 atggatacta ggaaacgctc tagtgattct tcattttgaa actgctcttt tctgcggctg
     2101 tatttcttga tttgtttatt gaaagattcg attgtagaac cagaaggaat ggagaacact
     2161 tttgaacttt acaacaaaac tactgttgat aaattaattc caattttagt tccaactgat
     2221 ttaaatggtc atgatgtttt acttcgtttt aacggatttt taggtgagga ttatagatac
     2281 ctaaaaattt ctgatcctca acctttaaca gaataataaa aaacgtgtaa tcaattattt
     2341 gattacacgt tttttatttc aatcttcgtt gtagtagtgg ttgcgacata ccgaaaggta
     2401 atcttcatta cctcctacta caatctgttc acctgaataa actggaatac catcaacaat
     2461 acgagcattc attacagcct ttttattgca aaaatggcaa atagttttaa tttcctcaat
     2521 tttatcagca aatgtcaata atgcttcgga tcccttgaag agattatttt gaaaatcatt
     2581 cttcaatcca tagcaaatta ctggtacttc ctcattcatt acgataagtg atagatcaat
     2641 aatattctct ctacttaaaa attgagactc atcgaccaaa atacaatcaa cttttccgta
     2701 ctgatcaata atctccacta attctccgta cacatccatt tcagaagtaa ttgcaatagc
     2761 tggtttcgaa acacctatac gactctcaac ttttccaaat cctgatcgag tatctattgc
     2821 gcttgtcatt atgataactc tcttcccttg ttcttcataa ttatgagcca ctttcagtag
     2881 ctcaatactc tttccactat tcattgttcc atatctgtaa aataattgtg ccattaaatt
     2941 tattcccttc ttttacaatt caattattcc ttaattccat ctgcttttat gcagctgctc
     3001 gtttattcct tgatatgtct ataacatcta ctatccctaa aaccaattta taagccatta
     3061 gagatggtat tcctgaacat ctctcatatt gttttttgag tggtatatca tctgaaaaac
     3121 tgaaggaatc atgaaaatag cgacatactt atcatttaga gttctaactt taaatgcatt
     3181 tacttcttca gtcagcactt tagtcatgtt ggacatggtt tggggcgtgt agtgatgccc
     3241 atacattttc tcaatcaagt ctgcgatttc cgacatggta ataccttttc ggaagaggtg
     3301 aatgacggtc tcctctaatg tgtcattcgt ccgtctataa gcaggaacag tctgttgctt
     3361 gaactcgcca ttgcggtcgc gcggaatctg gagatgaagt tccccatact cggtcttgac
     3421 cgtacggtca taggagccgt tacgagaatt acccgtgtta aaaccaatgc gatcgtactt
     3481 ttcgtaatcg aggaaagccg ttaactccgt tttgagaagc gtattgacag ctttttccaa
     3541 atggacacgg aataatccat tcaaatcgcc tttgttcgct agagtcttta ggatttctgt
     3601 agtaaaatcg ttcatatgga agtcctcttt tctgtgaatg tgttgtggta actctattct
     3661 acagaaggga cttccttttt tctatttaca caaaatattt tacactctca acaaaagtat
     3721 cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac cgaggtactt
     3781 tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga atacgatata
     3841 aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt tgttgtcagt
     3901 tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga attttgcttg
     3961 ctatactttg tcttgtagaa aagaggtaga caaaataatg caagctcaaa taagcgattt
     4021 tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa tatgaataaa
     4081 aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt gactgtttat
     4141 gagtatccga cctttgcaac atcacaatta catttggttt tctttcttct tttgggaggt
     4201 attttatggt ttctaccagt tgctttatgt gctgcagaaa tggcaacagt tgaaggatgg
     4261 aaagatggtg ggattttcag ttgggttagt caaactttag gagaacgttt tggttttgca
     4321 gctatttttt tccaatggtt tcaaattaca gtaggtttta ttacaatgat ctacttcata
     4381 ctaggagcgc tgtcgtatgt aattaatttt ccagagctta atacagatcc atttatcaag
     4441 tttattggtg tattagtcat tttctgggta ctgacttttt cacagttggg cgggaccaat
     4501 cgtaccgcta aaattgctaa agcaggattt gtcattggaa ttgttattcc ttcgatcatt
     4561 ctctttggtt tagcagcagc ctactttatc ggcggtaatc cgattcaaat ggctattaat
     4621 gcgaaagctt ttattcctga cttttctcag ctttcaactt tagtagtatt tgtatctttt
     4681 atattggctt atatgggcgt agaagcttca gcatcacata ttaatgagct ggaaaatccc
     4741 caaagaaact acccattggc aatgttttta ctagtgattt tggccatcgc cttagatgca
     4801 atcggcggtt tctcagtagc agcggttatt ccacaacatg acttgtctct cagtgctggt
     4861 gtaatccaaa catttgaaac gcttatttta cattttaaca gtcatttaca ttggttggta
     4921 aaagtgattg ctctcatgat tgcctgtggc gttatgggtg aagttagttc ttgggtagtt
     4981 ggtccatcac gagggatgtt tgcagctgct cagcgaggtc tgttaccagt tttcttccgg
     5041 aaaactaata aacaagacgt tccagttccg ttgattatga tacaaggaat tatcgttact
     5101 atttgggcag cagtcttaac ttttggtgga ggaggcaaca atttatcatt cttagtagca
     5161 atctccttaa ctgttgtcat ttatctcgta ggatatttac tatttttcct aggttacttt
     5221 gtattagttt ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg aaaaactgtt
     5281 ggcaaaacga ttatcggtgg aattggtttt attttatcag tgttcgcatt gcttatctct
     5341 tttgtaccac cagcttcaat tgcagctagt gagcaccgaa cataccaaat catactactt
     5401 attagctttg tagtgactgt tcttttacct tttatcattt atgaagtaca tgataaaagt
     5461 gctcacacaa ctattgagga acctactcac gtgaaagctg aagacgttaa tcccgctgtt
     5521 tatccagctg ctagaggaga gcaccacgtt acaaaaaatg atgaacatat tttaaaacat
     5581 taggaggaat tcattatgtt atacggaaaa gataatcaag aagaaaaaaa ctatttggaa
     5641 ccaatttttg gctctgcaag tgaggatgtt gacttgccta aatataagtt aaacaaagaa
     5701 tccattgaac cacgaattgc ttatcaatta gtacaagacg agatgttgga tgaaggaaat
     5761 gcgcgattaa acttagctac tttttgtcaa acgtatatgg aacctgaagc agtgaaattg
     5821 atgacccaaa cgttagaaaa aaatgcaatt gataaatcag aatacccacg aacaacggaa
     5881 attgaaaacc gctgtgtaaa tatgattgct gatttatggc atgctccaaa taatgaaaaa
     5941 ttcatgggaa cttcaacgat cggctcttca gaagcctgca tgctgggtgg tatggccatg
     6001 aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc aaaaaaacct
     6061 aacctggtga tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt atattgggat
     6121 gtggaactga gagaagtccc aatggatgaa aaacatatgt caattaatct agatactgtc
     6181 atggattatg tggatgagta cacaattggt attgtaggta ttatgggtat tacttacact
     6241 ggtcgttatg atgatatcaa gggtctgaat gatttagttg aagctcacaa taaacaaact
     6301 gactataaag tatacattca tgttgacgct gcatcgggtg gcttttatgc accatttact
     6361 gaacctgatc tagtttggga ttttcaattg aaaaatgtta tctcaattaa ttcttcaggt
     6421 cacaaatatg gtttggtata tccaggtgtg ggttgggtct tatggcgtga ccaacaatac
     6481 ttaccagaag aattagtatt taaagttagt tacttaggtg gagaaatgcc aactatggct
     6541 atcaacttct ctcatagtgc agcacaacta attgggcaat actacaactt tgtacgctat
     6601 ggctttgatg gttatcgtga tattcaccaa agaactcatg atgttgctgt ttatttagcc
     6661 aaagagatcg aaaaaactgg tatttttgaa atcattaatg atggatcaga attgccagtt
     6721 gtgtgctata agctgaaaga agatcccaat cgcgaatgga cactatatga tttatctgat
     6781 cgtctgttaa tgaagggatg gcaagtccca gcctacccat tacctaaaga cttggatcaa
     6841 ttaattattc aacgcttagt tgttcgagca gactttggaa tgaacatggc tggtgattat
     6901 gtacaagata tgaaccaagc aattgaagag ttgaataaag ctcatattgt ttatcataaa
     6961 aaacaggatg tgaaaacgta cggctttact cactaaaatt cttcagagaa atataaaaga
     7021 agttaattaa ctaataaaag gagttgtgat taccacacag ctccttaaat ctatttgaat
     7081 aaatatatga tattcatttg ttacactctc aatcttagct gttgttctat tcatcattac
     7141 tcttattgta agtagttcag tcaaatttgc ttgttcaatt gatcgataac agtatgaatt
     7201 gtttgaaaag taatcggata tataattgtt ttctcactac ctaagccttc ttggtataaa
     7261 gaactatcgc aaagtataat ccgctgagta caaaagaaag tgtcgtctac tatttctcta
     7321 agttgtttac aaatgatatt actaatagaa aagtgttctc ccaaaatgtc ttctaactgt
     7381 tgtcgatagt ataaatacct aaagtctgac ggagctacta tgaacacttc aattggagat
     7441 ttccgtttat gctcatttaa aagacttaaa ctaatggtta gtttacgaat agaattctca
     7501 ttccaacgca gaaataaatt gttttgtttt cgccattgtt caagaatttc atgaacttct
     7561 aagtacaaag cgcgttgctc ttcatttaac aagtaagtct tatcaggttg aaatatctga
     7621 atatcagccc aaagtgagcg gataaacggg agaagtgctt tatcaaaaag tatctgattt
     7681 tcttcagata tattcagaga taagataagt ttttgactga gctcaatcac caaagggtta
     7741 tctgctacat ggttttgata aacaacgttc aagtcttttt gtaacaattc aaagttatcg
     7801 catagataat ttctagagtt gaatagaaag tagatataaa ggcattcatc ttcgtcatga
     7861 cataaagaga aagaaagttg ctctaatcct ttctgtatta gctgaaacaa gggagtatgt
     7921 cgggcaactc ttttttcttc ctgactaaaa tatattgggt tagtcttaga tctctgcaaa
     7981 ccgatagcga tacctaataa aataaattgt ttttcaactg tagggtaact tcttgaaaaa
     8041 aatgtttttc tacataatca atcaattgtt gaccgctttg aataattgat ttatttagct
     8101 ggatcaactc tgtccgattt gtctcataga ttatcgttag taacaaactt cgataatcct
     8161 tttcaggaat ttcaaaatgg ccatcagcat ttaagtactg catttgttta aaaaaattaa
     8221 tcaactcatt tcgtaagttg taaactttgc ttaacgaaat gaaggtagta tcagctatat
     8281 tactccaatt gtgttcccct gataaataat gtcctagtac attgaaaaag tcagagcgct
     8341 gatagatgag ttgagtgagt gccaacctcg aaaacgaaga gtcgaatgcg actgtaaagc
     8401 tacttctaga tttctccact gaaataattt gctgatggag tatgtcggta atgcattcca
     8461 agtcgttagt gacagtgagt gggcaaacgt tcaattgatc tgctatttcg cgaatctgaa
     8521 tggtcccttt ttcgtgtaat tgctcacaaa tgtaaacttg acgaaaaata cttttctcca
     8581 gataggtttc caacatagta ctcctcctag tcattttata cataactatt ataactctac
     8641 caatcggaat agtcctgtaa tatattgaat caaaaatata aattataaaa taattagatg
     8701 gggtatttgg tttaagtatc ccttatatag taataaattt ataggtgtga ttctcgaatt
     8761 aatacccgaa aatgtgagag aatttttatg gattttttag gatacaaatt aattattttt
     8821 gcttttatcc tgattaccag tccagtactg tcggtatccc attttgcagc aaaatcatgt
     8881 tgataaagtg acaagaagac tgatgagata tattatccaa attgagaaga taattaatcg
     8941 gattcttgag tataccctac tttttaagaa agtagggtat acaagcgtat gaataaagac
     9001 ataactttcc ctgtgatgga cgttcggctt gttccaatta ccaaaataca ggcgaacgat
     9061 tataatccca atcgagtagc gtctccggaa atgaacttat tggagctttc tatcattgaa
     9121 gatggcttta ctcaaccatt agtttgttat tacaaggaaa aagaagagaa gtatatcttg
     9181 gttgatgtgc ttcataggta tcgtgttgct aaggaaagac tcaacttaaa agttgtaccg
     9241 gtaacagtta ttgataaacc catagaagaa cgtatggctt caacaattcg acacaatcgt
     9301 gcaagaggga cccatgaaat aagaggaatg gaaattgtca tccaaagatt agtaaaaaat
     9361 ggatggtcag acaagagaat atccaaagaa ttaggcatgg acattgaaga aatttttcgc
     9421 tttaagcaag tgtctggtgg aatggcaaca gtttttttga caaattttat aaggtgcaga
     9481 acttctttcc gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat
     9541 cttcggcagg agctaacagt taaagttaga ctgcctcgct agcgttagca catccgagct
     9601 cattatcaag gattcgctgc gccaatctct ggttacggta accaaccggt gtctcgtagc
     9661 caagtgtacc gtgcaaccga aggtggttcc accaatttac atagtcaaat aactccaaat
     9721 ccaattgttg taaggtttca aatgtgtatt gatagacaaa ttctactttc aacgacttat
     9781 aagttgattc agctacggca ttatcaaaag gacagccttt atgactcaat gatcgattga
     9841 tgtcaaaagt tgttaataat tcatcaatag cttggttatc aaactctttt ccacgatcag
     9901 tatgaaaaat ctcaacctct gtcagaggtt gtttgatacg gctaaatg
//
LOCUS       DANKHJ010000094         9838 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000094 DANKHJ010000000
VERSION     DANKHJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(79..2199)
                     /locus_tag="PW113_002592"
     CDS             complement(79..2199)
                     /locus_tag="PW113_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2526856.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="PW113_002593"
     CDS             2429..3607
                     /locus_tag="PW113_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2526857.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="PW113_002594"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="PW113_002594"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2526858.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="PW113_002595"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="PW113_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2526859.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="PW113_002596"
     CDS             complement(4960..6213)
                     /locus_tag="PW113_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2526860.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="PW113_002597"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="PW113_002597"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2526861.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="PW113_002598"
     CDS             7790..9136
                     /locus_tag="PW113_002598"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2526862.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="PW113_002599"
     CDS             complement(9444..9830)
                     /locus_tag="PW113_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2526863.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3141 a         1790 c         2012 g         2895 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatc
//
LOCUS       DANKHJ010000095         9798 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000095 DANKHJ010000000
VERSION     DANKHJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..809)
                     /locus_tag="PW113_002600"
     CDS             complement(<1..809)
                     /locus_tag="PW113_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2526864.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHS"
     gene            1010..2224
                     /locus_tag="PW113_002601"
                     /pseudo
     CDS             1010..2224
                     /locus_tag="PW113_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2403..2732)
                     /locus_tag="PW113_002602"
     CDS             complement(2403..2732)
                     /locus_tag="PW113_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2526865.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(2722..2994)
                     /locus_tag="PW113_002603"
     CDS             complement(2722..2994)
                     /locus_tag="PW113_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2526866.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3445..4248)
                     /locus_tag="PW113_002604"
     CDS             complement(3445..4248)
                     /locus_tag="PW113_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2526867.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4226..5350)
                     /locus_tag="PW113_002605"
     CDS             complement(4226..5350)
                     /locus_tag="PW113_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2526868.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5366..6181)
                     /locus_tag="PW113_002606"
     CDS             complement(5366..6181)
                     /locus_tag="PW113_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2526869.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(6171..7043)
                     /locus_tag="PW113_002607"
     CDS             complement(6171..7043)
                     /locus_tag="PW113_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2526870.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(7128..8417)
                     /locus_tag="PW113_002608"
     CDS             complement(7128..8417)
                     /locus_tag="PW113_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2526871.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8645..9289)
                     /locus_tag="PW113_002609"
                     /pseudo
     CDS             complement(8645..9289)
                     /locus_tag="PW113_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         3023 a         2061 c         1602 g         3112 t
ORIGIN      
        1 tcagagtgtg aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga
       61 ttgatgtgtt ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagactgtt
      121 ttaagcaact gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg
      181 gcttttctag gaaagtgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg
      241 atcgtaaata gctgatgggt tgtcccatca acacaaataa aactcatgga accagaaaca
      301 gacttcattg atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtgac
      361 tggtaatgct tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac
      421 ttcttagcga ttagtttacg tgattgattt tcgtacagtt ccatgagaat tgcctgcttc
      481 agttcctttg ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtcctt
      541 ccacaatcat ggcagagaaa acgggtacgt tttaattcta aaatagttaa cttttctttg
      601 actttcaata tctgagtgcg tgtcgtatag gaaccgttgc ggataatacg aaccgatcga
      661 cagtgttcac aataggaagg agagtcatcc aattttcctt taataaactg cgcttgaacg
      721 cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat
      781 ccgagtaatt ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg
      841 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
      901 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaagcaa aatcaattct
      961 accttagatg ttcttatatc actctatcag tcctatttga caaagagcca agaaaaagac
     1021 gttggtcgtc gatgctgttc tctcgcctac tcctcgtgct tgtagaagtt gtggttctac
     1081 tgtggtagat ggaaacggga aagcaattat agtgaaaaat gggaagaagg aaacgattgt
     1141 ccgttttgaa caatacaatc atatgccttt ggttacgcgc ctaaaaaagc aacgctatac
     1201 ctgtaaaaac tgtcgtaccc attggacggc tcaaagttat tttgtccgac tcagccattc
     1261 aatcgcagat catgttcgat ataaaattgc ttctttactg actgaaaaag tatctttgtc
     1321 ttttattgcg aaaagttgtc aggtatcttt gactaccgtt actcgtacat tgaaagagtt
     1381 taaaagctat ttaccaaagc cttctaagaa gattctcccc agagtattga tggttgatga
     1441 atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag atggtgaaac
     1501 aggaaactta attgctgtct tgcctacgcg taaattacct cgattaacaa gctatttctt
     1561 aggctgcacc aatccagaag aagtagaatt cttagtcaca gacatgaacg ccgcttactt
     1621 ccagcttacc aaacgtgttc tgccaaatgc gaaagtagtg attgatcggt ttcatattgt
     1681 caaacacatg aatcaggcac tcaatgagtt gcgtatccgt gaaatgaatg agcttcgtaa
     1741 agcaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttc tgctaaaaaa
     1801 tcgtgcaagc atcaaccatt atgagtacaa aacatggaaa agtttccgag caccaaaata
     1861 cccatttctc actgaagcaa tgatgattga tcgattgctt gagttttctg cgcctctaaa
     1921 ggaggcgtat cccttttttc atgaattagt cgaagccttt cgagacaaag accctgactt
     1981 atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc gggagaagct
     2041 tcaaaacctt ctgacctatg aagaaggtat caccagcgca atgatctatc cttattccaa
     2101 tggaaaaatt gaagcgaaga atacccacat aaagacaatg aaacgagtat cctacggatt
     2161 taaatcattc gagaacatga gaattagaat ctttttgatc aatcaattaa tcaatgtaag
     2221 ataacaaaaa atctgaacca gaatgtggtt cattctgacc cggatttgat tttgcacaac
     2281 tcatcagtcc ttattgacaa agagccaaaa atctgagcca gaatgaggtt cattctgacc
     2341 cagatttgat tttgtacaac ccatcagtcc ttattgacaa agagcccttc tttttagtat
     2401 tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact taattcccca
     2461 atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt tcctacgtca
     2521 atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac gtatttcagc
     2581 tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga aattgtgtcc
     2641 agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc tgtgaaagca
     2701 acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt caattttttc
     2761 gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat tatggattcg
     2821 tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa caatttgctt
     2881 ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa tgctttctgc
     2941 ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct ccacctcttt
     3001 ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac tacttttgaa
     3061 ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc taaatggcct
     3121 tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac actcaacgac
     3181 acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt ctgaaaactc
     3241 cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag accgatatgg
     3301 ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt attgatagcg
     3361 attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa agtgaatgaa
     3421 gtctttattt ttaatcaata atttttaatg attaccagta attggttttt tcatgcgccc
     3481 tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc cgtcaattaa
     3541 actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt attcatctac
     3601 accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag cactgctacc
     3661 ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca attcagcaaa
     3721 accttccgga acttcagtta caatagatgg atcttcccac gacaatggat gatgaaccaa
     3781 taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa tcatctgtcc
     3841 tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa taattcgtaa
     3901 cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa cactcattcc
     3961 ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag gtgtggttgg
     4021 gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc cttcatgtac
     4081 taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt gtaacaattt
     4141 ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa acaagttagt
     4201 ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg ctattccctc
     4261 tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa tcaaaaaaat
     4321 gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa gtaggttttt
     4381 cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa ctttgaccta
     4441 atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa aaggttacgt
     4501 gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt ttttgccaag
     4561 ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca taagtaactg
     4621 gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt ttaggtttcg
     4681 tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat aaaacaatcc
     4741 ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa gtttgtttat
     4801 aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc acatccaaat
     4861 ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca cgagccaatg
     4921 ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg tatggcgtta
     4981 aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc ttaattttat
     5041 tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat aaagcataat
     5101 tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct agttgtgtac
     5161 ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc agcaaagtag
     5221 attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc acagctaaat
     5281 caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt aattcaagcg
     5341 tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa gtcccgataa
     5401 tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt aaactaatcg
     5461 ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc atcgccaacg
     5521 gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac tcattccaag
     5581 aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga ggaaaaacaa
     5641 ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct agaaggctca
     5701 ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca gcaaattgtg
     5761 gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac aaataattag
     5821 gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag taccagattt
     5881 tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct acgcctaact
     5941 taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg ataattggaa
     6001 gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc aaactagtaa
     6061 tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac aataatggat
     6121 agataataat gattatgatg aacataaata agagatgtgg caaccagcgt ttaattttca
     6181 tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc aaatattaat
     6241 aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa agccttttga
     6301 taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt gatgatctta
     6361 atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa atatagtcct
     6421 gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg tacacgatcc
     6481 aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa aatgacaatg
     6541 aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat tacccaacct
     6601 tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac agcaattggt
     6661 cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa agaaatcaca
     6721 gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac tattcctaat
     6781 aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac ggtataggac
     6841 aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac atagttggca
     6901 aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact caaatagatc
     6961 gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg cgccagatac
     7021 aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc aaaagcagac
     7081 gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta tttgagtaac
     7141 tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc tgataaaatt
     7201 ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc aggaaatgaa
     7261 ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg attttcagct
     7321 aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg taaataacca
     7381 gtaccttctg tccatttagc taaagattca acactataaa gatattttaa gaaatcccaa
     7441 gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc accagcaggt
     7501 agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt aaaagtacta
     7561 tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac gttaccatca
     7621 ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc ttttaccatc
     7681 ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc tttgccatct
     7741 tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt atcagcaggt
     7801 tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga aaaagtttga
     7861 acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa tatatcttga
     7921 tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt accatctttt
     7981 tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc attatccgga
     8041 tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc aaaataatct
     8101 ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac agaagcttgt
     8161 aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac cttcactttg
     8221 tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc tagggtatcc
     8281 gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt tgtcttggta
     8341 ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac ccctataaca
     8401 agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca tttttctaat
     8461 caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa ataattcgaa
     8521 atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag aataaagccc
     8581 ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga ttataaaata
     8641 gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag gttatattat
     8701 aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc catacagaaa
     8761 atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat ccttcatgaa
     8821 tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc aaatctgctt
     8881 cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa aatatatgct
     8941 ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt tgtggccaga
     9001 tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct tttaaatcac
     9061 agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc tcaagggtta
     9121 ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca tctgtttgct
     9181 gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga aatgcccagc
     9241 tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt ttgacccctc
     9301 ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt aatttcttgt
     9361 taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct tgcatgctcc
     9421 ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc aattaagtag
     9481 ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta ttccttcttc
     9541 ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc aatggcttat
     9601 tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa gtctttgata
     9661 caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag cgattggtcg
     9721 atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc ccacaaaaaa
     9781 agctgtcctc acagacag
//
LOCUS       DANKHJ010000096         9663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000096 DANKHJ010000000
VERSION     DANKHJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..649)
                     /locus_tag="PW113_002610"
     CDS             complement(<1..649)
                     /locus_tag="PW113_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2526872.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMI"
     gene            974..1390
                     /locus_tag="PW113_002611"
     CDS             974..1390
                     /locus_tag="PW113_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2526873.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1387..1857
                     /locus_tag="PW113_002612"
     CDS             1387..1857
                     /locus_tag="PW113_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526874.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1871..2644
                     /locus_tag="PW113_002613"
     CDS             1871..2644
                     /locus_tag="PW113_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526875.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2658..3470
                     /locus_tag="PW113_002614"
     CDS             2658..3470
                     /locus_tag="PW113_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2526876.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3524..4489
                     /locus_tag="PW113_002615"
     CDS             3524..4489
                     /locus_tag="PW113_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2526877.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4605..7328
                     /locus_tag="PW113_002616"
     CDS             4605..7328
                     /locus_tag="PW113_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2526878.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7341..7973
                     /locus_tag="PW113_002617"
     CDS             7341..7973
                     /locus_tag="PW113_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2526879.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8872..>9663
                     /locus_tag="PW113_002618"
     CDS             8872..>9663
                     /locus_tag="PW113_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526880.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1476 c         1884 g         3043 t
ORIGIN      
        1 caatcatatg gaagccaccg attaggtatt ttatgggttt agtcggaaat gtttcaagag
       61 caatttccat catatcgata atgccatgat gactacagcc agtaaagaca atcagaccgt
      121 cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca aatttatcat
      181 ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg atgatatgga
      241 agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc attttaagga
      301 tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa taatggggta
      361 aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat cttaacccgc
      421 cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc aaagtttccc
      481 ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag agatatttct
      541 tccctgagta gttaattaag agagataacc catgtccact tttaaagttc aaatttattc
      601 ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca tgaacgtgta
      661 ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa aaaatattaa
      721 tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat gtggcggggg
      781 attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct ttaaataccc
      841 acactttcat caacactata atttagggaa agaaaagtgt tggcatgata tagaaaaacc
      901 tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa gcgtaaggat
      961 aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg ctcaaggatt
     1021 aaagcaaaca gctacaatga ttattggaga tagtgtacca attcatgcct tgtcagcatt
     1081 tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca caatagatat
     1141 agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg atatgctcac
     1201 tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac ctttagttat
     1261 ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc ttattaaaga
     1321 gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta ctggaggaga
     1381 tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca ggtagtattt
     1441 tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga ggcagcgaat
     1501 gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa attaacaatt
     1561 ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa cgaaaatatc
     1621 atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta tcctaaagta
     1681 caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt tagtaatgcc
     1741 attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct tggcattaaa
     1801 caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat gttataaagg
     1861 aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt tttgtatttt
     1921 agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca gtacgcttgt
     1981 tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt tggaattgat
     2041 gtccttagga ttagtaaata tcggagctgc tgcaccacct gatatgaata tggcatcaat
     2101 tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat taacgattgc
     2161 cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga ttttgtcaaa
     2221 tttgactcac gttgcggatc gctttatcga acaagggaaa tttagccaag caaggagaat
     2281 tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta tttttctagc
     2341 aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg agtggctaac
     2401 aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt tgttattaca
     2461 aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa ttactgctta
     2521 ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt ttgtcatggt
     2581 tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat tagatgactt
     2641 ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat cggcagttct
     2701 tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg aacatgggtt
     2761 ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa gatgaagcaa
     2821 tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct caaacaagtg
     2881 catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac caagaaaact
     2941 ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta tctggtgttg
     3001 gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga attaatttag
     3061 cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt gtaccttcct
     3121 ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa tacttgtcta
     3181 aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt gtggggttga
     3241 tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt caaatgggta
     3301 aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta ccattaggat
     3361 taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg atgttggcaa
     3421 tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa gggaataacc
     3481 aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt caaaagaaat
     3541 tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg gctgtggcgc
     3601 ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga agcagttacg
     3661 gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg tagatgagtc
     3721 aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg aagctaacgc
     3781 agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg actcaccgtt
     3841 aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg ctgctaaatt
     3901 agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag tagaaggtta
     3961 tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg cccaaaaaag
     4021 tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag atgaaaaagt
     4081 tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat caatttgctt
     4141 gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat ttttccacgg
     4201 accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg atggaaaaac
     4261 tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga aaacagaagt
     4321 tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct tagactactt
     4381 taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt cagaagcaag
     4441 agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa aaatagatca
     4501 aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt gattttatac
     4561 atatggtaaa ataactagta catattgtga aagcaggtgg caagatgcag cgattaaatg
     4621 actatcttta tcaactaaca ttgcttttga aatctacgcc ttcagataat tattcagctc
     4681 gcagtatttc gcaagcttta cagttgaata gaagtacaat cagtagctac ttaaatgaag
     4741 gtgttcgtga agggcttttc ataaaagtaa aaagttaccc ggttttattt ttacatagaa
     4801 cagctcttga agaactacat atcacactga ataactccga aatagaatca attgaaagct
     4861 tgttaacaat ttctcaaaaa cccgcattag atcaagtaat tggctctaaa ggaagtttga
     4921 aagaagcaat tgatcaaatt aaaacggcag tcttatatcc tggaaaaggg ttgcctttat
     4981 tattgatcgg cgctagtgga tcagggaaaa cctttctagc gaataaaata tatgaatatg
     5041 cagtagaaga aaaggtcatt cacacatcgg cacctttcat agattataat tgtgcacaat
     5101 atgtgagcaa tccagagtta ctatcctctg ctttatttgg ctacacgaaa ggagcattta
     5161 ccggagcaag ccaggagcat actggtttgc tagaaaaagc cgatggagga gtattgtttt
     5221 tagatgaagt gcatcgctta tctgaagaag ggcaagagaa attatttaca tttatggata
     5281 cgggtgaatt ctctcctatg ggggataata gtattcggaa aaaagccgat gttcgattag
     5341 tctttgcaac aacagaaaat atatatacga ctttcttacc aacttttctt aggagattgc
     5401 cagttattgt taatctacct agatttcaac aacgtccgtc gtttgaacgt ctgtcattga
     5461 ttgatgaatt ttttgtttct gagagtaaaa ttttagctaa ggaactatct gtttctgatg
     5521 cattgattca tttcttaatg aatagtgatt ttgaaggtaa cgtggggaat attaaaaata
     5581 ttatcaagta tgcttgcgga aacgcatata tccaccagaa gagtcagcaa acaattcaag
     5641 ttaaattatt ggatatgcct ttagagtaca gttataaatt caaggagcaa ctaaataaac
     5701 ccagaaagaa aagagcagat cgaatctact atccagaaga aacacgacaa gtgcaactag
     5761 aaagaaacaa tcagcttttt gttcaatttt ttgaccaatt acttactgga ttctctgaag
     5821 tgattgaaaa taacaaggat attaaactat ttttagaaga aatggttacc aaggtaacac
     5881 aagtaatgga tacgcttatt tttcaagagg attacgaaaa agagcagtct ttatacgcca
     5941 ttctaaacta tcatattcgt caaagtattg attttatgcg agaaagctac gggtttgaac
     6001 aagatggtaa ccgggtagtc gctttggcca actatctata tgtcaaggat aatcaagtga
     6061 tttttgaaaa tagtcttcaa gaaaagaaaa ccaaagatca gcttaacgaa ttttttgcta
     6121 atgttcttga aaccccttat tggtatgcaa aaaaaatctt aagcctttta tctgagtgtt
     6181 tagatcaacc tgtgtatgaa gaggacattt tctttattac gctatatttt tatagtttga
     6241 atgtcacgaa tgttaaaaca ggtattaaga gtattgtgct agctcatggt tattctacag
     6301 ctagtagtat ggcgaatgtt gccaaccgta tgctgaataa gaacgttttt caagcttatg
     6361 atatgccttt agatattaca ttagagaaaa ttgaagcaaa attgattcaa ttccttggag
     6421 attatcgaac agattcaggc ttgattttat tggtagatat gggttcacta aaccaactag
     6481 gcggtttatt agtgaatcat ctaaatggac cactgttaat cattgatcat atcaatacac
     6541 cgttattatt ggaagtcggc caacaagtat taaacaataa aagtatcacc gaaataggag
     6601 cagaaattga aacaagtctt actttcaaaa agcagttact acttcccaag aaaaagaaaa
     6661 agaaagccat cattacttgt tgttatacag ggattggtag tgcgacccag attcaggaga
     6721 ttttaacgaa atgcttgaaa gatgcagtta gagaattagt aattattccg tatgattaca
     6781 aaaagctgtt agaaaacaag aattttgaga ctccttttca attatacgat gtcatcatga
     6841 ttgtaggtac agaagatccc aaaattaatc agattccata tattggatta gatcgcctga
     6901 ttaatgggga agcaattggc gaatttgttc attacttgga aaaagagctg actattgaag
     6961 tgaacgattt acaaaaagaa ttaattttca atttttcaat tagtaaaatt gtcgaaaatt
     7021 taaccatttt ggatccagat aaggtgctat cttttgtaca aaaagcaatt aaagaattat
     7081 cgaagttatt aaattttgat ttttctaata atcagctgtt tttgttgtac cttcactgta
     7141 gttgtatgat tgaacgaatt gtacgtaaag aaagtgtgga tgagcaagag gacattgaag
     7201 aatatatgca agcaaatgga gaaataatgg ataaaattca tagcgctttt tttgagattg
     7261 aaagggaata cacaattgaa ataccagatt tagagttgcg attattgagc gatattgttc
     7321 aaggatagga gtgaattcga atgacaatgg gagtaatttt tgatatggat ggggtactca
     7381 tagacagcga gacattttat tttgagagaa gaatgaactt ctttcaagaa ctggagatag
     7441 atccaggatc aaaccagatt gatgattatg tggggaaaac agaagctgca atatgggaaa
     7501 cattagttcc tgaggacaaa tcattaagag aggaacttta tccgaaatat gtagattatc
     7561 gaaaaacgca tccaattgat ttcgctaaag atttacgaaa agaagtgaaa ccgttacttg
     7621 acttcctcaa gaagcgggat ataccattgg cactggcatc ttcttctcca gttgttgaga
     7681 tagagagtat gctcattcaa tgtgaactta ctgaatattt ttcctttgtt ataagtggtg
     7741 agtcattaaa acaaagcaaa ccacatcccg aaatatattt aaaagcgaaa cagcaattag
     7801 actgtgactt ctttattgca gtagaggatt caacgattgg tattaaatca gcaaaggcag
     7861 caaatatcta tacggtggca ctaaaacaac caatgcaaat cgatcagagt gaagcagaca
     7921 tacaaattga aagtctacaa gaactggaac aaattgtttt agaaaaaggt tagtgaatta
     7981 atggaaataa ggctatatat ctgtgctagc tattttgact aaccaaaaaa acttgctata
     8041 attcaggtgt agtaaaaggt gctggtaaca ccttttacga aaatgcttcg gcagtgataa
     8101 tgggattggc tttttaagtt ttttattttg ttcgcaatcc ctaaggtcat gtgaatgatc
     8161 ctagggattg ctagaatatt ttgcaacatt ctatctattt cagaacgtaa ccttccccag
     8221 ttcattggga ttcattagca tacaaccaac atttcaaaaa gaaattgcac tacaccatta
     8281 ttagctattt tgactaaccc caaaaaactt gctataattc aggtgtaggt aaaaggtgct
     8341 ggtatcacct tttacgaaac tgcttcggca gtggtaatga gtgtggctta aaggactagt
     8401 cctttaagct ttttactttg ttcgcaatct ctaagatcaa gttaatgatc gataagattg
     8461 ctagaatatt ttgcaacatt ctatttcaga acgtaacctt tcccattcca ctgtgattca
     8521 ttggcaaccc tcctcacaac caacgtttca aaagaaattg taaagttgtt ggggtaccac
     8581 gaccgaacaa tactacacct gaatgatagt aagttgtctc attaagtata gcatatcctc
     8641 tttttgggta aatagaaatc ggttacattt tttatccaag accaagtttt ttcatttggt
     8701 cttatttgct atacccaaac aattccgcag tatagtcgac ggtattgatt ttatcaggtg
     8761 agtaatagaa acttgttttc cttccccaaa acattaccta taaaggagaa aaaatggtat
     8821 cgtaattata taggtatagg atgaaatttt tttctaagag gagggttgtt gatgaaaaat
     8881 ttacagttaa aatttattgt tgatccatcg attgtacgct ggtttcggat tatcaatcaa
     8941 tttgagcgca atcaacttgc cacaaaagga gaattagcaa agagtaatca tgtatctgaa
     9001 cgaaccattc aaacagatgt caataaaatg aaggactact tttctgaaag tgttcaattt
     9061 gtttcaacga aaagtgggta ttcttttcag aaggaaagac cggttctttt tctcaagcag
     9121 aaggagcaac tactggagaa cgaaatttta tttgagttgt tagggaatgt cttttatggc
     9181 gaattggaag atatgacgga gctaattgat cgttttcatt tatctgaagc gacttttcgt
     9241 cggtatttga agcagattca accagttttg gaagaatacg gcttagaatt gagtctttat
     9301 cctcttgatt taaaaggaga agaggcaaat attcgcaagt tttttaaaga cttctattat
     9361 gaaggggaga tgacgcccca tactcttgtt ccaccaaggg atcttcatga acttgtgcaa
     9421 gctacattct atgataaatt tgagtatttc tcgattggaa cagggacttc tcctgctgaa
     9481 ttttattata gtttgtatat tgcgattgag cgtgtgagac aaggaaaaac gatagccatt
     9541 cctcaaggtc tgattgagcg agtaattgct agtgagaatt ttcgcttaat gtattccttg
     9601 aaagaagcga ttcaaacaaa ttatcaagtt gctttatcag aagaggagtt ttgttggttg
     9661 tat
//
LOCUS       DANKHJ010000097         9640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000097 DANKHJ010000000
VERSION     DANKHJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..199
                     /locus_tag="PW113_002619"
     CDS             <1..199
                     /locus_tag="PW113_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2526881.1"
                     /translation="TPEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLI
                     KNNLKYPVEKSAGVRKKYTEL"
     gene            267..980
                     /locus_tag="PW113_002620"
     CDS             267..980
                     /locus_tag="PW113_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2526882.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            1290..2140
                     /locus_tag="PW113_002621"
                     /pseudo
     CDS             1290..2140
                     /locus_tag="PW113_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            2261..4126
                     /locus_tag="PW113_002622"
     CDS             2261..4126
                     /locus_tag="PW113_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2526883.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            4174..5583
                     /locus_tag="PW113_002623"
     CDS             4174..5583
                     /locus_tag="PW113_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2526884.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            5691..6392
                     /locus_tag="PW113_002624"
     CDS             5691..6392
                     /locus_tag="PW113_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526885.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            6551..7030
                     /locus_tag="PW113_002625"
                     /pseudo
     CDS             6551..7030
                     /locus_tag="PW113_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            7878..8453
                     /locus_tag="PW113_002626"
     CDS             7878..8453
                     /locus_tag="PW113_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2526886.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            8453..8893
                     /locus_tag="PW113_002627"
     CDS             8453..8893
                     /locus_tag="PW113_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2526887.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            8909..9610
                     /locus_tag="PW113_002628"
     CDS             8909..9610
                     /locus_tag="PW113_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2526888.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         3230 a         1568 c         2130 g         2712 t
ORIGIN      
        1 aacgccggaa gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt
       61 attgatctat tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga
      121 agaaaaattg atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa
      181 aaaatacaca gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca
      241 gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag
      301 tcaatatcct cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga
      361 tcaaggatta ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag
      421 aagatctgaa aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa
      481 tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta
      541 gtatcgcagt cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt
      601 cactacttta cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg
      661 ccttagcaag ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag
      721 agatgggtgc caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt
      781 ttaaagaaaa agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa
      841 ttgtcacact aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag
      901 aactggaaag tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc
      961 tagagacaaa aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa
     1021 aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg
     1081 catctctgat ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat
     1141 gaaaaggctt gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg
     1201 ttactgtacg gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt
     1261 ttttattttt cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg
     1321 tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt
     1381 ttcaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgtttaag
     1441 agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg cagaggagat
     1501 cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg aattttcgcc
     1561 taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag caaaagaaaa
     1621 tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga aagaatatca
     1681 agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac tttctgagga
     1741 tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg aagacatgca
     1801 agtgacgatg accgctacca caatggtaag agatatactg agtatcatca gtaaattctt
     1861 cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc atctgcagtt
     1921 ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat ttctctacgc
     1981 attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca atgagtatct
     2041 tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa cgattcatat
     2101 tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac tgtatggcag
     2161 gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt ttcaatctgg
     2221 gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc aagcagttgg
     2281 tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag tacattgtgc
     2341 tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa agttaaaaga
     2401 agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag tgattggcag
     2461 taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag tttcggagga
     2521 tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct ctagtatatt
     2581 tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct tatcattggc
     2641 aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt ttgctgcagc
     2701 agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta aaaagttcaa
     2761 agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag ctatcacagc
     2821 agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc tttcaccatc
     2881 tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa gtaaattaga
     2941 gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc cgcttgtcgt
     3001 tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga cggttgccgg
     3061 taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg tagcaggtct
     3121 aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg gattcgtacc
     3181 gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa tgcttcttcc
     3241 agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa aagatacgaa
     3301 acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca ctgaaccaac
     3361 tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta tttctggagg
     3421 tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca gtttggtcag
     3481 cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa atgtgacagt
     3541 agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat tgattttagg
     3601 atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg caggagaacc
     3661 gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac tgccattgag
     3721 tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg ctattgatcc
     3781 agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt ttccaactgg
     3841 acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata tcgggatgga
     3901 tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg atctcgtcaa
     3961 agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg gctatagcgt
     4021 aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg aattagacca
     4081 aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag gaaatcagga
     4141 tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc aaatcaatta
     4201 gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat gcctggaggt
     4261 aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga tcaagaaaaa
     4321 tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga agatcttgcc
     4381 ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac acgaattttt
     4441 ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga tcagctgatt
     4501 gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta cgaaatgcct
     4561 cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga cttctatgaa
     4621 cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg gatgactttc
     4681 aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt aaaaagcaat
     4741 ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt tgcaagtagc
     4801 aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg catgattatc
     4861 tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac aatgatccaa
     4921 aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata ccctgcatac
     4981 acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca aacggaaaaa
     5041 gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta ttatatgtct
     5101 acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt gctgggagga
     5161 gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc agaagggctg
     5221 agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat tgttgaaaac
     5281 ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga ttatcggatc
     5341 gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg tgtagatttg
     5401 atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg agaaatgagt
     5461 aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac actgaatcga
     5521 tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg cgtaaacttg
     5581 taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt gttctttttt
     5641 tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt atgaaaagga
     5701 cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc tcaggaaaat
     5761 atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt caagtcagca
     5821 aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc gcaaagaaaa
     5881 gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc aaagcggcaa
     5941 gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg ctggatttgc
     6001 aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag caagctgttt
     6061 gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt cattatcttc
     6121 cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg tatcgtttgc
     6181 tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca gtatttttaa
     6241 cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa cggatacgga
     6301 aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac catacggagc
     6361 tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg aaaaagctgc
     6421 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcgtttggtt
     6481 aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg gataaggggg
     6541 tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt tagcttcgat
     6601 ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc gtaaaaatat
     6661 actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga tctgtcagat
     6721 cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca tagcaacggg
     6781 agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata gccgtattcc
     6841 ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca ttggtgcatt
     6901 gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac cagtagatcc
     6961 gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg atgatttgac
     7021 aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg taccttgaga
     7081 caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt ttggtttagg
     7141 agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga aggaaaaatg
     7201 agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag gaaacaaaaa
     7261 aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa aaagaaatta
     7321 ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag ttccaggcag
     7381 gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca agtgatattt
     7441 ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg atgaacaaaa
     7501 atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac atcatttatc
     7561 agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taaaagaaac aagtatttgt
     7621 tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct tctataagta
     7681 agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata aaatggctga
     7741 aaaatgattt ttttctaaat actatatata gtggcgtcta tcctatatac cactaaaaaa
     7801 ctacaaaatc tagtctttta ttttaaaaaa acacgtgtag aatgaagata gacttttgag
     7861 ttagggagtg tttttttatg aaacttactg ttttttgcgg ttcccgtttt ggtaataaag
     7921 aaagttacaa atttattgcg caaacgttag gaaaatacat ggcacaagaa gaaatcgaac
     7981 tggtttacgg tggttcatac tccgggatta tgggagtgat tagtaaaaca gtactggaaa
     8041 ataacggaaa agtcacaggt atctatccga atgggctttt tgaaactgaa ttgcctggaa
     8101 aagatgttac tacatttatt cctacagaaa cgatcgatga aagaaaggta ttattatttg
     8161 aaaagggaga tgctgtcctc gttttccctg gaggtctagg tacattggag gaattctcac
     8221 aacttctttc ttggatagca atcggtctga cgcccgacaa gccaatcgga atattgaata
     8281 ttggcggtta ttacagcggc ttgcaacaat tattagaaac ttttgcaaaa gaggatttta
     8341 tggataaaaa atggttggat caagtaatct tttccaacaa tccattggaa ctggtcaatg
     8401 aattacgagc agtatctatt aaaaatcagt tattattgaa ggaggcaaaa taatgagtca
     8461 aatatattta gcaggaccat ttttttcaga ggagcaaatc gatcgagtaa gtcgtatcga
     8521 aaaagcgttg gaggaaaaca aaacagtcac aagtttctac tctccacgtc atcaccaaga
     8581 aagcaattat gaactattca gcgctggatg ggcgcaggaa gtatacgaaa aagatatgga
     8641 agaactgaca aatgcagaat ttgttgttgc tatccttgac tttgagcatc aaacaatcga
     8701 tccaggaacg gcttacgaat taggtgtggc aactatgttg aaaaaaccga tgatcattgt
     8761 acaagaagag acggtaccta cgaacttgat gattacacaa agtctccata cgtatttaaa
     8821 atcagaccaa gctgtacgtg aatatgattt tgaaacattg cctgttgaaa cttacgtagg
     8881 cgagtactta tagaagagga agggttgtat gtcagtgatt ttgatttcag gaacgattgg
     8941 tgcaggtaag agcagtttga cggatatgct tgcaaaagag attgattcta aaccatttta
     9001 tgaaaatgtc gaagataacg aagtattgcc gctcttttat tcaaatcctg agcagtacgc
     9061 ttttttattg caaatcttct ttttgaacaa gcgattttta gcaatgaaaa atgctttagt
     9121 gaacgatgac aatgtgctag accgatccat ctatgaagat agcctgttgt tccatctaaa
     9181 cgctgattta ggtcgggtaa cggatatcga agtacagcag tacgatagct tattagatac
     9241 gatgctaaac gagttagatg atgttgctcc aaaaaaacgg cctgatttga tggtccatat
     9301 caaagtttct ttggacacga tgctagaacg tatcaaaaaa cgtgggcgtg actatgaaca
     9361 gctagaaagc gatgagacgt tatataccta ttatgaaacg ttgaacaccc gctataatca
     9421 atggtatgaa gattttgatg tatgtcctaa aatacaagtg gatggggata aattcgattt
     9481 tgtagaaaat ccagaagatg caaaaaaggt gatccgtcag atccaagaaa aattagctga
     9541 attggaagga aagcctgctg gaaaatattt tacggataac cagcatgcaa aaggtcaaga
     9601 attaggctaa aaataaaaaa ccaaacgacg ggaaagaaaa
//
LOCUS       DANKHJ010000098         9580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000098 DANKHJ010000000
VERSION     DANKHJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            119..1108
                     /locus_tag="PW113_002629"
     CDS             119..1108
                     /locus_tag="PW113_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2526889.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="PW113_002630"
     CDS             1090..1551
                     /locus_tag="PW113_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2526890.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="PW113_002631"
     CDS             1601..2065
                     /locus_tag="PW113_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526891.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="PW113_002632"
     CDS             2079..2828
                     /locus_tag="PW113_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2526892.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="PW113_002633"
     CDS             2828..3658
                     /locus_tag="PW113_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2526893.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="PW113_002634"
     CDS             3658..4305
                     /locus_tag="PW113_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2526894.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="PW113_002635"
     CDS             complement(4403..5179)
                     /locus_tag="PW113_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2526895.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5323..6282
                     /locus_tag="PW113_002636"
     CDS             5323..6282
                     /locus_tag="PW113_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2526896.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6399..6707
                     /locus_tag="PW113_002637"
     CDS             6399..6707
                     /locus_tag="PW113_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2526897.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6734..7033
                     /locus_tag="PW113_002638"
     CDS             6734..7033
                     /locus_tag="PW113_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2526898.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7207..7758
                     /locus_tag="PW113_002639"
     CDS             7207..7758
                     /locus_tag="PW113_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL2526899.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7904..8197
                     /locus_tag="PW113_002640"
     CDS             7904..8197
                     /locus_tag="PW113_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526900.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8190..8486
                     /locus_tag="PW113_002641"
     CDS             8190..8486
                     /locus_tag="PW113_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526901.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8557..9561
                     /locus_tag="PW113_002642"
     CDS             8557..9561
                     /locus_tag="PW113_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2526902.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3391 a         1371 c         1791 g         3027 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ctaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaaa tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaacg tcgattgtaa aattaagcta gacaactaaa aagatcattt gtgctacact
     5281 caaaatagtt ccgaccaaag aattaataag gagtgagtac aaatgactta cacccatctt
     5341 acaacagacg aactagtaat catagagtct tatttcaata tcgagaaacc tgtcgctctt
     5401 gtcgcacaaa gtttaaatcg ctctagacaa accatttata atgtttattc gtttctcaaa
     5461 caaggaaaaa aagtgctaga ttattaccgt caatacaaac aaaataaatc tcgttgtgga
     5521 cgacgttcaa ccgttttatc tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa
     5581 ggatggtcac ccgatgtgat tatcggccgt ggcgagtttc caattgattg ttctgttcgt
     5641 acactctatc ggatgtttaa aaaaggcctt tttgacacac agtcgttacc tatgaagggg
     5701 aaacgtaaac cgaataatca tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt
     5761 catgaacgga acagtctcta tccacacctg actaaagaat ttggtcatct cgaagtcgac
     5821 accattgtgg gagttaaaca caagagcgct gttattacat tggttgagtg tttatccaag
     5881 gttattatta ctttgaaacc gacaggcaga cgagccgaag atatcgaaaa caggttaaat
     5941 gattggttca aatccatgcc taaacacttg tttaaatcca tgacatttga ttgtggaaaa
     6001 gaattttcca actggcgatc tataagtaac tcgaacgata ttgccatctt tttcgcagat
     6061 ccaggaacac cttcgcaaag agggctaaat gagcactcaa acggcttgct tagacgagat
     6121 ggcttaccta aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc
     6181 tcgaaaagaa accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt
     6241 ttgagctgca tagataaaga catattgtct agcttaattt gacaaatcag atagtataaa
     6301 atgaatcaaa ttgaatatca aatatatatt atatccatga agcccaatct tctttaatag
     6361 aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat aaaggacaac
     6421 gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct ttatgttgga
     6481 atctaatcga tcaaaacgtt caaaagcaat ttcatttaga ctatcttcaa atctttgaat
     6541 tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaacca gaagcaagaa
     6601 aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa atatgggtca
     6661 ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat ctaaaagaaa
     6721 ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg cacagctagt
     6781 cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg aaattgaaat
     6841 gggtatgctt cctgaagatg ttactctacc acgtttagtg atgacacatc tttatattta
     6901 ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata ttaccagcca
     6961 gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc agattggagg
     7021 aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa gatatcctta
     7081 cagcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt ttcaactagt
     7141 attgacactt ataacggcaa tggtgattga attgcttgaa caaaaattag cataggagaa
     7201 aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg agatcctcat
     7261 catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga agcggaagat
     7321 aaattagcgg tagtcattac tattcgtcca acagatacag aaccatttac gaatgatttg
     7381 agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt tattgctgtg
     7441 aatctttttt cgtatacaaa agcgaaaagt cctcgggtat ttcctcgtgg aaatgatgaa
     7501 caatctttgg aaatattaac aactgttttc acagaaaaga aaatcaacac cattattttt
     7561 gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcgaa gaccatttac
     7621 gatcaattga gtgcgaaaca aaaaaagatg accaaagtat tgataaatgc agaaggtaag
     7681 ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga tagtcaatta
     7741 ttatttcaag aaaaataaca ttttattttc ttatgttact tataagtagt tttacatgct
     7801 ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat gtacccagta
     7861 atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga ttgatgaaac
     7921 ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg aactggattc
     7981 agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt ttccaatgaa
     8041 aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac aggaaaaaac
     8101 tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga ttcaaaaagt
     8161 aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta ggtggtttaa
     8221 tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata gataaaggaa
     8281 aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact aagaaaaata
     8341 atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga agggaaattt
     8401 ctactagttt taatccatct gaaagccctc aaggaagaga tcgttgtcat gctggctatc
     8461 gacattcttg ggaaaggatt aactaaatga acaccattga gattaattga ctgataaaat
     8521 aagtacgcga gggagaaatt gaaaggggga aataaaatga aaaaatggtt gttattacta
     8581 attggaatgg ggattttatt cactggttgt acatctgaga atacgaggac taaagagact
     8641 gtagaaactg atacaacaat tgaggctaaa aaaagtaaaa cgcaatcaac tgaacaaact
     8701 acaacagaaa tgaaacaatc tgtggaagca agtacaagtg aaagttcaat cattgaatct
     8761 tcttctgaag aggttaaaaa tactttgtgg gatagtgaga aatctactca actagcaatt
     8821 tttgtcccac aatggggaaa gacattaggt caagaatata aaagctacaa tcctcagaac
     8881 aatgtaagtc tgtatggtac gcctttacca agtgcagtta ttgatggaaa ttggaaaatg
     8941 gcaatcaatg aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat
     9001 ttcaatttag tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgta
     9061 tatttctttg gctttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggc
     9121 aatgataaaa actacatgta cttcaatgaa actcagaacc aacagttaaa acaaggtttc
     9181 attgatattg tgaatggaca aactcctcaa actccagtag tagaagaatc tactcaacaa
     9241 gcaacaacta caacgacaca atctacaagt agctcagcaa tttcaagttg ggaagctgct
     9301 aaagaaactg tactaaataa taaagcttta tggttgtatg attctcctga taaaaatgca
     9361 tcaaatacta atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat
     9421 ggaagctact attcaattaa tattgtaacg gatgataatg gaaatacaac aatggctggt
     9481 ggaaaacaat tccgtgttta tacagatgga agaatttctc aacgtattgg aatgactaac
     9541 aattttcaaa tgattaaata attgaaaata ttatttatca
//
LOCUS       DANKHJ010000099         9388 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000099 DANKHJ010000000
VERSION     DANKHJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1143
                     /gene="ecbA"
                     /locus_tag="PW113_002643"
     CDS             <1..1143
                     /gene="ecbA"
                     /locus_tag="PW113_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family collagen-binding adhesin EcbA"
                     /protein_id="HDL2526903.1"
                     /translation="ENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLN
                     RLMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAID
                     VDATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVY
                     ESKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMF
                     DDVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVD
                     TSKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTV
                     PSGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            1255..1569
                     /locus_tag="PW113_002644"
     CDS             1255..1569
                     /locus_tag="PW113_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2526904.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            1582..1956
                     /locus_tag="PW113_002645"
     CDS             1582..1956
                     /locus_tag="PW113_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2526905.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            1957..2310
                     /locus_tag="PW113_002646"
     CDS             1957..2310
                     /locus_tag="PW113_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL2526906.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            2379..3395
                     /locus_tag="PW113_002647"
     CDS             2379..3395
                     /locus_tag="PW113_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2526907.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            3989..5863
                     /gene="ltrA"
                     /locus_tag="PW113_002648"
     CDS             3989..5863
                     /gene="ltrA"
                     /locus_tag="PW113_002648"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2526908.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            5989..6345
                     /locus_tag="PW113_002649"
     CDS             5989..6345
                     /locus_tag="PW113_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2526909.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            6390..7532
                     /gene="dcm"
                     /locus_tag="PW113_002650"
     CDS             6390..7532
                     /gene="dcm"
                     /locus_tag="PW113_002650"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDL2526910.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            7522..7811
                     /locus_tag="PW113_002651"
                     /pseudo
     CDS             7522..7811
                     /locus_tag="PW113_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8067..9251
                     /locus_tag="PW113_002652"
     CDS             8067..9251
                     /locus_tag="PW113_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2526911.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            9248..9361
                     /locus_tag="PW113_002653"
     CDS             9248..9361
                     /locus_tag="PW113_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2526912.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3122 a         1596 c         2105 g         2565 t
ORIGIN      
        1 gaaaataaag aggattatac caagagtgat tatatcttta ctattaccga aaaagggcaa
       61 aagcaaccga ttaaaacggt aacggtccct tatgggaatc taaccaatga gaatttctca
      121 gtcagtttga accgtttgat gctttatgat ttgcctgagg aagaagatag cttaacttct
      181 cttgcgactt ggaaagaagg agataaagaa ttgactactc ttgattctac cgagctagtc
      241 gataaattga gctataatct acatgaaatc aaagaagact ggtatatcgt agctcaagcc
      301 atcgatgtag atgctacaaa agctgcccaa gaaaaagata aaaaagccaa accggtggcg
      361 attgcggaaa caaccgcaac tttagcgaat aaagagaaaa caggaacttg ggagattctg
      421 cataaattaa ctgtcgaaca agttttagac aagaccattg tcttattcaa ctatgtgtat
      481 gaaagcaaag aagcctatga agctggtgat gagccggtag cgaaggatgc tagcttgaac
      541 aatcaagcgc aaactgttaa ttgtatggtg gaacgtcatg tttccatcca aaccaaagcg
      601 cacttagaag aaggttctca gacctttact catggtgacg tggtagatat gtttgatgat
      661 gtatccatta cccatgatgt actagatggc tcaaaagaag cttttgaaac aattctgtac
      721 gctttatttc cagatggtac aagtaaagaa atttggaaat ctggcaagat tgattacgaa
      781 gtgaatgaca aagaatttac caaaaccgta cttgcggaaa aagtggatac aagtaaatat
      841 ccagaaggta caagttttac cttcaaagaa attaactatg acaaagatgg gaatatcaat
      901 ggaaaacaca atgaagattt gaaagaaaaa tctcaaactt taacgcccaa aaaagttccg
      961 acaacaccaa gtacaccaga acaatctgaa atgccaactg ttccaagtgg ctctcaagaa
     1021 tctagtccta cagtgaaggc attccctcaa accggggaga aaacttccaa tgtgctactg
     1081 ttcattggct ttagcttgat ctttgcgacg gctggctatt atttttggaa tcgtcggaac
     1141 taaggtgata agatgaaaca tgtaaatagt agaaaacaat acgcgcgacc gcctcctaaa
     1201 aaagtggtcg cgctattttt gtattcaaaa ctaacaatga aaagaggaaa aaatatggaa
     1261 ttgaaatttg ttgtaccaga tatggtcgag acgttcggaa aactaacata cggtggtgaa
     1321 ggtgaagttc tatcggaagg gtatggtcga aatactacaa ttgttggtcg aagttaccat
     1381 ctgttttcaa ataaacaacg ggccgatgat attgaagtgg tggtagctgc ggaagccggc
     1441 gaaaaggatt ttgaccaaga tcaaccacta aaagctgtaa atccccactt aattgccaaa
     1501 ggctatgaaa ttgagaatcg tgggtttacc gattacgtgt tgtatgttga tgatttagtg
     1561 aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggatta
     1621 acctttggca agttgcgttt ttccgcatta cgtcgagaag tacgcaagca aaatgaggac
     1681 ggaacggtta gtaacgaggt aaaagaacgg acctataatt tgaaatcctc cgcgcaaggt
     1741 cgaatgatcc aagtcagtat tccagcaaat gtacccttac gcgagtttgc ttatgatgca
     1801 gaggtagagc tggtcaatcc aatcgtggat acggtcgcca attatgtttt tcgggaagga
     1861 accaccgtta attggtttat caaggcagat gatttagtct tgaaacgaca actgaatcaa
     1921 ggaaaaccta cgaaccaaaa tgaaggaaag aaataggtga agaatatgga aattaatgtt
     1981 gaacatattt tagattgtct cgatcaattt ggcaaaggtg agctaacgga ggaacaactc
     2041 actaacgcat taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa
     2101 gtaggtaacg atgccaaaga agactttgat gtactggatt ggttagggga agaagagtct
     2161 ttctttatgg ttgtcgaggt aaatgaaagt ttatgtcggc aggcagaatc tgttttggaa
     2221 gaaatcgatg tggagatgcc ggatgccatc gaaagatttt taaaacaatt agtggaaacg
     2281 aaacaacttc ccgtagtagt aaatgactag cataactttt tgaaattgcc caatgaaatc
     2341 ctattgggct ttttcgactg gaggtggaaa ccatcaaaat gtacaaagga catcgcatac
     2401 gcgcaggaga tcaacacttg atttatcact ttgttcttgg ctggctaatc tttttgttca
     2461 ttggttggat gagcgtcttt tatttacaag aacttagaca atttgatatt tctaaggtat
     2521 cattttccac tagtgaaatt ggctggtcta tgaaggatgt aatctgcttg ctaggaagtc
     2581 tcgctctttc aggaagcatg atgttacttt atatacactt ttttcaggat cattggcgaa
     2641 gtctttggca tcgacaaaag ttagctcgga tgattctaga aaaccattgg tatgaagtaa
     2701 agcagactca aggtgaaggt tttttcaaag atttgaatag tagttggacc aaagagagta
     2761 tcagttactt ccccaaaatt tattatcgaa tgaaagatgg cttactttct attcgcgttc
     2821 aaatttcact gggaaaatat caggatcagc tattgaagtt ggaaaataag ttagaaagcg
     2881 gactgtattg tgagttagta gaaaaagaac tcaaagactc ctatgtggaa tacaccttgt
     2941 tatacgatat gattgccaat cgaattggga tagaagaaat agtggcagaa aatggggcgt
     3001 tgcgattgat gaaaaatcaa gtatgggcgt atgattcctt gccgcatatg ttaattgctg
     3061 gcggcacagg tggtgggaag acctatttct tactcaccat tattcaagca ctattgaagt
     3121 cagatgcaga attgtttatc ctcgatccca aaaatgcgga cctcgccgat ttaggtacga
     3181 taatgcctca cgtatactcc caaaaagaag aaatttctga atgtgtggaa gatttttacg
     3241 aacgcatgat agctcgtagt aggtcaatga aagaaatgcc caactacaaa acaggagaga
     3301 attatgcgta tctcggactt ccgcccaact ttttaatctt tgatgaatac gtggcttata
     3361 tgggtgcgaa tcgttttcct acaagcatcg aatagatgtg aaatggaagc ctattgagat
     3421 tatcaatagt gacaactaat gttttgagag atggaatgaa agccttcaag ttgagctgaa
     3481 acatccccgc taatactcct acatgctcta atcaatgaag tagattggtt ttggtatgaa
     3541 gctaggtgaa gtcgtctgaa atctgcctaa gtgcttgagc ataaggctga ctatagggcg
     3601 ggcggctgaa aaagataact atggtaagaa tgtctgaaaa tgactgacga atctgcgaat
     3661 gtacgggtct aaaaggctga tcttattgaa agataggaac atacggacgt atggtgagat
     3721 accgacaagt aaggttccat gatatgaaaa tcgggatagc taacgaagaa gctataattt
     3781 cacaggctca tagtagatac ctaaggttat acatgatagc taaaaacatt ggaacgatgt
     3841 aagcagaaaa ctatcaatta aatgactact tctagatagg tgcatataag taataacgaa
     3901 attgctttgt ttctgtgaaa gtaagggcac agtaccgatg aagcgtctaa caaatgtgga
     3961 gggatagccc taagtctatt taataacaat gaatgttgaa acagatgaag ttcaaggatc
     4021 tggtaagaac gtgggaaaat ccgttatgga ggtgccacgc ttgaacacaa aaatacgtta
     4081 ctgggaatat tatgatctac aagaaacatt cgatcagcta tttactcaaa gtagaaacgg
     4141 gaaaaagttt tatcaattat atgagttaat catttctgaa aataatattc ttttagcata
     4201 ccgtactatc aaggcaaaca aggggtcttc gacaccagga acggattcct tcacaattga
     4261 taactataag gaaatgaacc aagcagagtt tatccatctt attctcagtc atttagaaaa
     4321 ttacaaacca aaatccataa agcgagtaat gatacctaaa cccaatggtg agaaacgccc
     4381 attaggaatt ccatgcatga ttgatcgaat gatccaacag atgttcaagc agatactgga
     4441 gccaatatgt gaagctaagt tttatgaaca tagctacggt ttcagaccat tgagaagtgc
     4501 aaagcatgct ctaggtcgca ttatgtatct cataaacatc agtaaaatgc actatgcagt
     4561 agacattgat atcaaaggtt tctttgataa tgtaaaccac cgtttactga taaaacaatt
     4621 atggaatata ggaatttgtg acaaacaagt cttagctatt ctttcaaaat ctctgaaatc
     4681 acccattcaa ggagaaggta tcccgagcaa aggaacgatc caaggaggta tcatttctcc
     4741 actattatct aatgttgttc taaacgactt agaccattgg gtctcaaagc agtggcatac
     4801 ctttgaaaca aagtatccct atactaaagg ttacaataaa tttcgtgctc taagagatac
     4861 aaatctaaag caaggctata ttgtcaggta cgcagatgac tttaaaatca tgactaatga
     4921 ttatccgaca gcattaaaat ggttccacgc tgtcaagctt tacctaaaag acagacttaa
     4981 attagatatt tccaatgaga agtctaaaat cgtcaatttg cgtaaatgta agtcggaatt
     5041 tttaggattc gctatttgcg tgaaacaaaa agggaaaaaa tgggtttgta attcacgtat
     5101 ttctaacaaa aaaaaggatc agattaaaga agagattaaa caaagaatca aagatattca
     5161 aaagagtcct acagcacaaa atgctctcct gtttaatgct ctcgtgctcg gtgttcataa
     5221 ttatttcagg tatgccactc atattaattt agatttgaat aggatgaact acgttctttc
     5281 attcactact tacaaccgat tatcaaattg tagtacaaga tgttacccca acaaagcttc
     5341 cccaacttac aaaaagtttt actcaacaac gatgaaaacg tataaagtag ctggagtcta
     5401 tttgtaccct ctttgcgacg tatctacaaa aacaatttac ggatttaata cggaggatac
     5461 accgtttact ccgtttggtc gtcaaagatt ggagcacaaa agcctacagt ctcttgtgtt
     5521 ccaagaatta agaaaattga tggaatcgaa aataccgaat agaacggttg agtatttaga
     5581 tagtagaata tctcgttact caatgaaatc aggcaagtgt gagattacaa aacaattttt
     5641 gccagcaaaa gccgttcact gtcatcacta tttgccaaaa agtcttggtg gagacgacaa
     5701 attcgataac ttgagaatta ttcataagga tatccatctt ctgatccata caacaaataa
     5761 aatgataatc gatcactacg tgcatgaact gaaactggtg ccagagcaaa tagcaaaaat
     5821 caacctttat cgaaagatgt gtaatctgca aaacattcaa tagaaaataa atagatggaa
     5881 cgccgtatga ggtgaaagtc tcacgtacgg tgtggagcgg gggaaaagat ggagattata
     5941 tcaaattctt acctatcgct atagatgtta acgacaaaag aaagtgcggt gattttgaat
     6001 aagcttaaac aaatcgtgat gttaggccgt caatctggtt tctttctgat tctagcttgt
     6061 caaaggccgg atgcgaaata tctaggagat gggattcgcg atcagtttaa cttccgtgtg
     6121 gctttgggtc gtatgagtga gcttgggtat tccatgatgt ttggagaagt cgataaaaat
     6181 ttttttatga aacgcatcaa aggacgaggt tatgtagata cagggggcag tgtgattagt
     6241 gagttttata cgccacttgt accaaaaggg tatgatttct taagggaaat tagcaacatt
     6301 gttggattaa ccgttcatac ggaaagagaa aataatagtg tgtagcctag gaatatcaat
     6361 aatcctgggc ttttttattg aggtgattta tgacattttt agatttattc gccgggattg
     6421 gtggctttcg cttaggtatg gaacaagccg gtcaccagtg tatcggtttt tgtgaaattg
     6481 atgaatttgc tcggaggagt tataaagcaa tccatgatac aaggaaggag gtggaaatgc
     6541 atgacatcac aagtgtatca gacgaatttg ttcgatccct cggaccagtg gacatccttt
     6601 gcggcggctt tccgtgccaa gctttttcaa ttgcgggaaa gcggcaagga ttttccgata
     6661 ctcgaggtac tttattcttt gaaatcgctc ggttcgccac tcttctccaa cctaagtttc
     6721 tattccttga gaacgtccgg ggattgctca atcacgaagg aggggctacg ttcgagacga
     6781 tcctccgaac gttggatgga ttggggtatg atgtggaatg gcaagtgctt aactcaaagg
     6841 cctacgttcc ccaaagccgt gagcggatct tccttatcgg acattctcga gatgcatgta
     6901 ccgaacaagt atttcctatc attggatcgt cttcaacatc tgatcaaaac atcagaaact
     6961 tggtaaatat caatccttcc aatcgaggaa tgggtggcca agtctatggt tcagatagcg
     7021 tagcaccaac cttgaccggt gatgaaggaa ttaaaattgc cttaccggta aaggatggga
     7081 tttctgtagc gggtatgttg tcgggaaatt ttgaacaagg caataggatt tatgaggtaa
     7141 ccggaacagc ccccactttg tcaacgaaac aagggggaac gaagattatg attcggaaaa
     7201 atgaaacaga atatcaagag gtgaagccag gggacagtgt gaacctggct tttcctaatt
     7261 ccacaagtcg ccgaggtcgt ctgggaaaac aaagtgtgca taccttacta acaggagacc
     7321 aacaagcggt agtcacggat cagtaccaga ttcgaaagtt aacacctagg gagtgctggc
     7381 ggcttcaggg ctttccagat tgggcttttg atcgagcttc tcaagtcaat tcagatagtc
     7441 agttgtataa acaggccgga aactcagtaa cagttccggt catttttgat attgctagaa
     7501 gattaaaggg ggtaaaagca tatgatttat gacgagttga ttggagaaat ctactggatg
     7561 attggcaaga ttcaggcaga tccgaaattg gaagaagaac tgtaccggct caattttgat
     7621 attcgcaaaa acggggtgaa agtccaggtg attcgtatgt agtggatgag gaaacggatg
     7681 ctcgaatgga gctcaatcaa gtcatcggtg aattggaacg aattgcggac cttgccaaag
     7741 aacctgatat ccgccaatac ttgtttgaga taaaagcaga gttggaaatt gagggtatca
     7801 ccgcggagta atagggaaat aacccactga taggcactga ccgcggggga tttgcgaagc
     7861 agaaatcctc cgtggtgctg tgcctgttca tggacggcgg cagccggcca tgatgtctca
     7921 ccccccgtat ctaacagggg ggtacaaaaa cacaaaaaac aagcaagtaa ccataaaaaa
     7981 tgcttgttgt atcaaggata ttaagtaatt tgtataggtg ttaactcatt tggaaaagtt
     8041 aacaccttga atttggaggt gaaaacttgg cgcaacgaaa tttagattat cggttattaa
     8101 aagaacgacg caacgaatat ggcgtttcac aaaataaatt agctactgct tgcggactta
     8161 gccgcccata cctaaatcag atagaaaacg gtggcgtaat cgcctctaca aagaccatga
     8221 gaaaaatttt tgatcaactg gaaagtttca atcccgattt acctttaacg ttactatttg
     8281 actatgtaag gattcgcttt cccacaacgg atgtgcggaa aattattcaa gaaattctcc
     8341 atttgaaatt cgagtatatg cttcatgaag attacgcctt ttactcctat caagaacaat
     8401 atgtcatggg cgatattgta gtgatgttat ctcatgaaga agataaaggt gtccttttgg
     8461 aattaaaagg tcgtggttgc cggcagtttg aaactttttt actcgcccaa aagcgtagct
     8521 ggtacgactt ttttgaagat tgtctaaaag ccggtggcgt gatgaaacgc ttggatttag
     8581 caatcaatga tcgagtgggg ctactggata ttcctgattt aacgaaaaaa tgtcagaagg
     8641 aagaatgtat ctccttgttt cgtaccttca aaagttaccg ttccggggaa ctgttgaaag
     8701 ctgatgaaaa ggatggtatg ggcaatacct tatatattgg cagcctcaaa agcgaggttt
     8761 atttttgttt gtacgaaaaa gattatgaac aatatatcaa gttagggatt ccattggacc
     8821 agaccgaaac gaaaaatcgt tttgagattc ggttgaaaaa tgaccgtgct tattatgcga
     8881 ttcaagattt gttaaaaggt cgtagcattg aaagtaccac gttttccatt atcaatcggt
     8941 atttgcgatt tgcggataaa gtagatggaa aacggcgaac caattggccc ttgaatgaac
     9001 aatggggtcg ttttattgga cggaatcgga aagagattca attaacttct gaaccgaaac
     9061 cgtataccat tgaacgaaca ttaaattggc taggtagaca agtcgcccct acgtggaaaa
     9121 tggctaagga gttggatcga ttgaatcaaa caacgtatat ccaggatatg gtacagaatg
     9181 cgcgactctc ggaccggcat aaaaagattt tggagcaaca aagtatggca atcgaaaact
     9241 tgattgtatg aaaagaggaa ttttgatgga aattgtaaaa gatatgttat tggtacttgg
     9301 tggcagtatg cttggtgtta ccgtgatgtg cttgatgaat gcaagtgcag cagcggattg
     9361 agcaatggaa aagaaaggaa agtgacaa
//
LOCUS       DANKHJ010000100         9348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000100 DANKHJ010000000
VERSION     DANKHJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..244)
                     /locus_tag="PW113_002654"
     CDS             complement(<1..244)
                     /locus_tag="PW113_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2526913.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="PW113_002655"
     CDS             complement(237..1829)
                     /locus_tag="PW113_002655"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2526914.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="PW113_002656"
     CDS             complement(2339..3064)
                     /locus_tag="PW113_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526915.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="PW113_002657"
     CDS             complement(3218..3544)
                     /locus_tag="PW113_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526916.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="PW113_002658"
     CDS             complement(3581..3946)
                     /locus_tag="PW113_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL2526917.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="PW113_002659"
     CDS             complement(3983..4198)
                     /locus_tag="PW113_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526918.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="PW113_002660"
     CDS             4327..4662
                     /locus_tag="PW113_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526919.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="PW113_002661"
     CDS             complement(4738..6735)
                     /locus_tag="PW113_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526920.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="PW113_002662"
     CDS             complement(6833..7108)
                     /locus_tag="PW113_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2526921.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="PW113_002663"
     CDS             7340..8074
                     /locus_tag="PW113_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526922.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="PW113_002664"
     CDS             8113..9255
                     /locus_tag="PW113_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2526923.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3060 a         1761 c         1358 g         3169 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacagg
//
LOCUS       DANKHJ010000101         9304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000101 DANKHJ010000000
VERSION     DANKHJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..89
                     /locus_tag="PW113_002665"
     CDS             <1..89
                     /locus_tag="PW113_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HDL2526924.1"
                     /translation="LLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(158..886)
                     /locus_tag="PW113_002666"
     CDS             complement(158..886)
                     /locus_tag="PW113_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2526925.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(899..2179)
                     /locus_tag="PW113_002667"
     CDS             complement(899..2179)
                     /locus_tag="PW113_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2526926.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2320..2922)
                     /locus_tag="PW113_002668"
     CDS             complement(2320..2922)
                     /locus_tag="PW113_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2526927.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3167..3373
                     /locus_tag="PW113_002669"
     CDS             3167..3373
                     /locus_tag="PW113_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526928.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3685..4404
                     /locus_tag="PW113_002670"
     CDS             3685..4404
                     /locus_tag="PW113_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2526929.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4485..5168)
                     /locus_tag="PW113_002671"
     CDS             complement(4485..5168)
                     /locus_tag="PW113_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2526930.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5387..7573
                     /locus_tag="PW113_002672"
     CDS             5387..7573
                     /locus_tag="PW113_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2526931.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7919..8329
                     /gene="spx"
                     /locus_tag="PW113_002673"
     CDS             7919..8329
                     /gene="spx"
                     /locus_tag="PW113_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2526932.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            8343..8699
                     /locus_tag="PW113_002674"
     CDS             8343..8699
                     /locus_tag="PW113_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2526933.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3354 a         1524 c         1740 g         2686 t
ORIGIN      
        1 tattgctgaa agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg
       61 aaattgcaca ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg
      121 gctggtcatt tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc
      181 cacggataga ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat
      241 taatcgaccg cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc
      301 gatgcatttt ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat
      361 ggacttccag tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg
      421 gtaaaaattt gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca
      481 tttgaaaatc ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa
      541 gatcttcttt gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca
      601 tttccgtatg ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt
      661 taccgatcgc tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac
      721 caaaagaaga agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa
      781 tatggacata actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa
      841 cttctaattg ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt
      901 attttatttg tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga
      961 aaagataagg attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt
     1021 ttggatttgt ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg
     1081 ttgttgtatt gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga
     1141 tttttttctt ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca
     1201 tacgtacaag gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct
     1261 ggacgatcaa ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct
     1321 caggaacttt catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc
     1381 gtgtaggtaa tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa
     1441 tatagctgtg aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt
     1501 gatagttttt ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata
     1561 actgatcagc taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa
     1621 cacacagata tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag
     1681 aaaaacgggt ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac
     1741 cgatcatacg gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag
     1801 ctaataaagc agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg
     1861 gttcaataac cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta
     1921 aaagcgacgt caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa
     1981 agaagaaaaa agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa
     2041 tcgaatagac ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt
     2101 tgtatcggat gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg
     2161 ctatggacca taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa
     2221 ttggcgtatt atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag
     2281 cagccagata caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg
     2341 aaagattcct ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa
     2401 aacaatccca agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag
     2461 agaaaaaatt agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc
     2521 aatgggattg attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat
     2581 taacaaaaag accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg
     2641 aacgccccat aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa
     2701 tccataagca tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca
     2761 tgagcctgtc accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa
     2821 aacttctaag acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct
     2881 gaataaggct tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc
     2941 acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac
     3001 atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa
     3061 gtgacagttt tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt
     3121 atgttaaaaa attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca
     3181 attgtttgaa gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa
     3241 aggaaaaata gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt
     3301 agaagacaat gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac
     3361 aatagaagaa taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt
     3421 tagctctgag ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc
     3481 cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga
     3541 cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt
     3601 agcttttaag gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga
     3661 agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc
     3721 ggtgcaggga aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt
     3781 tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat
     3841 acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc
     3901 agccatgatg acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg
     3961 aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa
     4021 acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat
     4081 attcgtgtat cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa
     4141 cagctagaac aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa
     4201 gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat
     4261 ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag
     4321 aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca
     4381 aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt
     4441 tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga
     4501 ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa
     4561 atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata
     4621 gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct
     4681 ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt
     4741 gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat
     4801 gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta
     4861 acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct
     4921 ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg
     4981 gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat
     5041 agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata agtataactg
     5101 tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa
     5161 gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac
     5221 gattgaaaaa aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt
     5281 tttagaaagg tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt
     5341 gaaataaaca aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga
     5401 aattctccga ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca
     5461 agtctttcgg ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc
     5521 cggacttatt gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa
     5581 aatgatggag aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata
     5641 tgatatgaag aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat
     5701 ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat
     5761 gatcactttt acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca
     5821 gagaaaaacc gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga
     5881 aatttgtcga aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga
     5941 tactgcacaa aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat
     6001 atttgatcaa tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa
     6061 cctttcagta gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa
     6121 tgcacgacag ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa
     6181 aagaagccag caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga
     6241 gaatgaggag atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt
     6301 tcaagatact aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat
     6361 caagttgatt gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc
     6421 agcatacatc aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa
     6481 ttatcgttct cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa
     6541 tgatcggata ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta
     6601 tttgttttct gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat
     6661 tcaaaaaaat gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca
     6721 gcagcttgtc caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc
     6781 atatgaaggt atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg
     6841 gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct
     6901 gatggcagac tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca
     6961 aaatatagcc gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc
     7021 agaaatccga gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc
     7081 gcttgttcaa agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca
     7141 agaatggata cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc
     7201 gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg
     7261 aaaacttgat gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa
     7321 tgtctttttg attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa
     7381 agtaatcgac tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag
     7441 actattttat gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat
     7501 gaaaaattca agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa
     7561 aaaaggaaag tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag
     7621 gaatgtacga aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct
     7681 atttctggaa cacagtttat tcggaaaagg gaggtgtgac aagaattttg tcacacctcc
     7741 cttatttatt ttgaaaatga gaatactatt atcaaaatag agaatatata gaaaaattct
     7801 cattattcaa acgtaaataa tcagttattt tgattttttt tatattttat tagctaataa
     7861 ttggtatttt aaaagaaatt actgtagaat tggattataa ttaaaaagga gtgagaaaat
     7921 gataaaagta tatacttcgc ctagctgtac ttcctgtaga aaagcaagag catggttgca
     7981 agcgaatcaa cttgaatttg aagagaagaa tatttttgcc gaaccgttaa cagaaagtga
     8041 gatcaaaaag atcttggcag cgactgaagg aggagttcaa gatattattt ccactcggtc
     8101 gaaaatttac gaaaagctgg gcatagattt taacgaatta actttaaagc aaatgatcgt
     8161 cttattcaaa gaatatcctt cattattgag acgacctatt ttgattgatg aaaaaagaat
     8221 actaatcgga tttaatgaag atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt
     8281 tgccagaaat ctgatctatt tacaaaacaa taacatctta aattcatagg gagggagcct
     8341 tcgtggaagt gaaagtctat ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat
     8401 ggttagaaca ccatcaattg ccttatcaat ttgttgactt agaagaacgc gatttaagta
     8461 cagaagaagc agaagaatta tgcagttttg aagaagtgca agcagaacag ctatttgcga
     8521 cttggtctga ggcttttcac aacattgcga ttgacctttc ctgtgtagga aaaaaacaac
     8581 tgattttttt atgcacacaa cataccaatc tcctcagacg tcctttgatt attatcgatg
     8641 aagttctatt tattggatac aatgagcagt tgatggaaca gcttatctta aataaataga
     8701 ctttaactgt tttaatcaag tcactgagca tttcatacag ttgtaacagt cgaacactta
     8761 gcatcaagcg tgttatgtgt tctttttttg actttatttt tctactatag agacatgctc
     8821 aatttatgtg gtcaaaattc aaatctaggt ggggatatag agttaaaaat atattgacat
     8881 gtgtaaattt acatgatata ttcatttctg ttgttttaag tagcaaagca ataaaatagt
     8941 attttaaaaa acatataaaa tagttgttga catgttagaa atgacatgat aagataactg
     9001 agttgttaaa aaagaaccac ttgtgaagct tgattctatc gaaaaaaact tcaaaaaagt
     9061 tcttgacaaa taacaaatga taagttatga taaatgagtt gctgtaagat ttcgaaaaac
     9121 gaaataaaaa aaacttttaa aaaagagttg acaattactt gtctctctga tatgatataa
     9181 aagttgctac ggcagccaaa aacgaatcgg ggaacactcc tgatgaagta gacctttgaa
     9241 aactgaacaa agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca
     9301 aaca
//
LOCUS       DANKHJ010000102         8751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000102 DANKHJ010000000
VERSION     DANKHJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..304
                     /locus_tag="PW113_002675"
     CDS             <1..304
                     /locus_tag="PW113_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2526934.1"
                     /translation="SEDTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTK
                     ESLDSLRAAGIVKLPEDLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            406..705
                     /locus_tag="PW113_002676"
     CDS             406..705
                     /locus_tag="PW113_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2526935.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            695..2371
                     /gene="mdcD"
                     /locus_tag="PW113_002677"
     CDS             695..2371
                     /gene="mdcD"
                     /locus_tag="PW113_002677"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2526936.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            2375..2968
                     /locus_tag="PW113_002678"
     CDS             2375..2968
                     /locus_tag="PW113_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2526937.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            2992..4068
                     /locus_tag="PW113_002679"
     CDS             2992..4068
                     /locus_tag="PW113_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL2526938.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            4093..4962
                     /gene="mdcB"
                     /locus_tag="PW113_002680"
     CDS             4093..4962
                     /gene="mdcB"
                     /locus_tag="PW113_002680"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL2526939.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            5073..5837
                     /locus_tag="PW113_002681"
     CDS             5073..5837
                     /locus_tag="PW113_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL2526940.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            6326..8419
                     /locus_tag="PW113_002682"
     CDS             6326..8419
                     /locus_tag="PW113_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2526941.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            8444..8746
                     /locus_tag="PW113_002683"
     CDS             8444..8746
                     /locus_tag="PW113_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2526942.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         2909 a         1532 c         1859 g         2451 t
ORIGIN      
        1 tagcgaagat actacacata tcgtgacaga agaaggaatt gcttatttgt acaaagcaaa
       61 aacaaaagaa gaacgacaag cagcaattgc agcaattggc ggtgttacac ctcttggaat
      121 gactagcacg aaagaatcac tagatagctt gcgtgcagcc ggcatcgtga aattaccaga
      181 agatctaggc atcaaacgta gtgaagcgaa acgttcatta ttagctgcac aaaccatcga
      241 tgatttgatc gaatggtcag atggcctgta tgaaccacca atgaaattca gaacttggtc
      301 ataaaattgg cgagaaaaac cttttgtcgc catttaccca gatggaagaa gaaagacgaa
      361 taagcagaac gtgtatgctc tgttttacaa aaaggggaga gatccatgga acaattagct
      421 tataaatatc caacgaaaca gccgatcaat aagaaaatcc atgtaggagt ggtcggttca
      481 ggagatttgg aaatcttgat gtttccaaca gaaaaaacag aatctactgt aaaaatttgt
      541 acaggaagcg atggcttcgg cgaagtatgg aacaatgtat taacgagatt ttttgatcgt
      601 tatccaattt cagcagatat cgtgatcaat gacttcggtg ctacaccagg agttgtgtac
      661 ctacgattga cgcaagcaat ggaggaactt tcagatgaaa aatagttttg tagaattaaa
      721 cgccagagaa cgagtgaacg ctctacttga caatggacgt gaattgattg atccattcga
      781 tcaaatcatc gcgccaagtt taccagcaca aggaatcgtc cctgaaagtg atgacgggat
      841 cgttgtcagc cgtggcacga tcaatggcaa acaagcagta gtaatcgcta tggaaggaaa
      901 attccaaggt ggcggtatcg gtgaagtttc cggtgcaaaa atcgttgctg cacttgaaca
      961 tgtgttagaa gaaaataaaa aaggaaatga gatctttcca gtcattgtac tagatacagg
     1021 tggtgttcgt ttgcaagaag caaactacgg actgttatct atttcagaaa tcggaaatgt
     1081 aatcattgct ctaaaacaat atgtaccagt catcggtttg attccaggac gtgtaggatc
     1141 atttggcggt atgtcgatta cttcagcatt aatgagttat ttaatcgcaa caaaaaaagc
     1201 gagagtcggt ttgaatggac cagaagttat tgaacaagaa gcaggcgttc gagaatttga
     1261 ttctagtgac aaagacttga tttggaatac attaggatct cgtcaacggg tagaatcaaa
     1321 aatcattgat gaattagtga ctgatagtgt agaagcaatc aaagctgctg tcatcgcagc
     1381 aatggacgaa cgaaaagaca cgcatcgcag tgaaagctca gcattttatt tatcattact
     1441 tgattcttta gatctttcaa aaccaatggc cattgacgat tacaatgaag caattgctaa
     1501 ccatcaagca aaatcaattg acttaccaga agtagcagaa ggaaatgaac cagctgcagc
     1561 aagtgttggt tacgattggt ttgcggcact aactggcatg aaaaatccaa cttcttctat
     1621 tagtactgtg tattatggta aatcaagtaa attcatagaa gaagccatcg tgacatcaat
     1681 cgtacctgat cctaaaaacc cgatgtaccg tgtacgaaac ggtgaagttg gtttagttga
     1741 aggtttccgc atgggacaaa ttttggatca cgtttacgaa gaagataaag acaaaaaagt
     1801 gaaacggcca atcatcgttg tgatcgatgt accaagccaa gcatacggct ataacgaaga
     1861 attgatcggt atccatgtgg ctttggctaa tagtgctgct gcttatgcaa aactccgtca
     1921 agctggacat ccagttattg gattgattgc aggaaatgct atttcaggtg catttttagc
     1981 acatggatta caatcaagcc gcttgattgc tttaaacaat aaagaaatta ccgtccaagc
     2041 gatgtctaaa gaaagtgctg cacgtgtgac aaaacgttcg gttgctgaaa tcgaagcagc
     2101 tgctgaaaaa gttccagcta tcgcttacga tatcaataat tatacaaaac taggagcgat
     2161 ctatcatttc ctagaagata tcaccgacca agctgcttca gaacaaaatg tcagcaaagt
     2221 catcgaagca atcaatgaag ccattcaaga tgtccgcaat acaaatgaca cgatgctgac
     2281 aaatcgatac acaaacgaaa cagccaaaac attcggacgt gtcgaaacaa ataaagtccg
     2341 tgccaaaatg gacgaagaat gggatgcata aaaaatgatt caagcacatg atattgtcct
     2401 ttttgataaa catgaaatag aagggttcac cttgcctgaa tgggtcgttc taactgaaaa
     2461 gatgttcgga acagttcgtc gtgcgtcgac ggatcacgca gaaatactac cgattggttt
     2521 acgtggacct gcccgcaatc agagatggtc aacagaaatc tcaaaacatg ccgtcaaaca
     2581 agtcattcat ccgtgggaca tcatcgaaca agaatcgtat agacaagaga aaatcatgga
     2641 ttatcctgtt tatcagcaat ttcatgctgc tagaaacata ttatcaggat gtaaatgggg
     2701 aatcggcggc agcttaggat ttgaattatc gactgggatt ccggcagtaa aagaaaccag
     2761 tgattttgat ttgctgctgt acgcaaattc accgattgaa ttgccaattc aggtaattca
     2821 atcgcatcct gctttttttg agcaattcga tacccaagta gtgactcggc aaggcggttt
     2881 ttcactaaaa gagtatttga gagagccaga gaaaaaaatt ctattgaaaa cagtcgaagg
     2941 acctaaatta accaaagaac tttggtaata aagaaaaaga gagagaaaga gatgtttgaa
     3001 aacaaaaaaa tcaaaatcaa cgtaagtgac ttccagaaag tatcagctgt aacggctgtt
     3061 atgatgcaaa cgatattgag ctttgctttg gcaaacagcc acagcgcaac aaatacagct
     3121 tttcttggta ccttttacgt catcgttaaa tacacagcac caatgtttat ttttgcaatt
     3181 gtttataata tggtaaaaac aagtcagcat ttgtcttatt gggaattttt gaaagaaaag
     3241 ttttttgaac tagtcgttcc gtatcttcta tggacgacgg cttatctttg gctatttcca
     3301 gcagtccaac aacaaactcc gtatacggat actgcaagct ttttactgaa atatgtcaca
     3361 ggtgatggag cgcctcattt atggtatacc gttatgatgc tgcagatcca attgttcatg
     3421 ccgttttttg tttggctcgg ttacaaagta tttgctaata aaaaatatgt atggccagta
     3481 ttgattggtt caacgatttt atatgtcgca tggtatctgt tctatcaaac acaagtattg
     3541 actgggcctt atcatgacag ctggtactta ttagatcggt ttgttgctag tttcatgatt
     3601 tatggggtag ccgcatttgt ttatcacgaa aaagtctatc aatatttgga cagagtacgt
     3661 tatcttttct tacctgttgc cttagttatc gcgttctttt ctgtccgttc attgctcgca
     3721 catccaggag acttgagctt tgcgaatgca ccttacttga atacgatcca aagtttatac
     3781 agtttagtca tcatctttgc tgtcttcata ggcgcttcca aaatgattgt aaacgattcg
     3841 ccaaaattgc cattgttcaa atggttgtct gtttatgctt atcgtacgta tttagccaat
     3901 gtatttgttt tccaagtact gttattattg tttaaagata gttggctgca gttaccttca
     3961 ggagtcatga ttcttgtagc ttatctaatg acggcaagtt gtgcatttgc tttaagctgg
     4021 ttacttcata tcatttgggt agctatcaaa aaaggtttca gcaagtaata gtaggaagaa
     4081 ggagtgagtg tcatgataaa tgacgaacga aaaaaagcag tgactcagca actcgctgcg
     4141 ttggtagagg aagcattgat cgcagaagta acattaagtc caaaacctgg attagtggat
     4201 gcactggatg cgggtgcaca ttccgatatg gactatgctt tatttcttaa gtctgctaag
     4261 acgctaactc ctttttttga agaaatggct caaatagcat ggcatcatgc tattgatcag
     4321 gagttaagag aaagaattgc tgagattggg cgaaaagcgg aaaaggcaat gctgattgct
     4381 acaaatggca tcaataccca taaaggagcg atttgggcaa tggggctatt gatcagtgtc
     4441 attgcacaaa aaatcagtat ggaacagcct ttttctttat cgaaaatact tgatgatgtc
     4501 agcaagcttg ctgcttttcc tgatacaaaa tatcgtcata aaaaaaccac tcatggtcat
     4561 agggtgaagc aaaaatatgg cgtaaatggt gcatatgaag aagcgatatt agggtatccg
     4621 catatcaggc ttgcaattca agcagctaag gagctagata atgattcata tgtacagaaa
     4681 caaatgcata tgttgttagt tttgatgagt tcgctagatg acacatgcgt attgcatcgc
     4741 agtgatcaga aaacgttagt ggaaatgcaa aaacttgctc atgctgctag tcaaagtgct
     4801 ttaccaaatc gtccatttac agcactgttg cacttttgtc aggaaaacca aatctctcct
     4861 ggaggaagcg cagatctctt gtctgcaagc ttgttcatgc tgaagataga ggaattatgg
     4921 actgccgaca catacatttg tccgatgtta ctcaatgact aaataagaca aaattgctaa
     4981 aaaaacagtt tatgcatctg gttattgact cttcctttta gcaaggctat cataaaaata
     5041 gttttttgta ataaaagata acgaggaaga caatgaaaaa aatcaattat ttagcaatca
     5101 ttttgttagt gggcctatgt ttgtttgctc tgccgaccca aggattcgct gtcgcgggtg
     5161 gtcatggtgg aggtacatct agcggtcgaa gttcgggcag aggttcattt ggtggtcgtt
     5221 catatggggg cagttatttt ggcagtcgtt cttacggcag agattcttat gggtattatg
     5281 gtccaggtac aggtccttct agttatgagc aaacgggatt tcttgcatta gctgttttgt
     5341 cagtaggagg ggtttctgct ttttctatcg tcaagaataa aagacaaaag aaaaggctga
     5401 aagcggagat attgtcccgc atgtcaggaa cggctaaaga aaagaaagca cagtttctgg
     5461 aaatccaaaa gatattcatg aagatccaat ccgcttggga agaagaaact cctgaaaaag
     5521 ccagaagctg ttatacagac aagctgtttg ccgaacatca gaaagttctg caaaaaaaca
     5581 aagcagacgg tgtaagaaat catactaaaa aaataactat tcaggaagta ggaaattatc
     5641 gacagatcca cgaaaatagt tttgccatcc gaattgattt cagatgctgt gattattcag
     5701 tcgatcgaaa agatgggcga ttgatctcag gaagtaagac acaacgacag tatttttctc
     5761 agatatggta ttttgatttt tctgagaaag aaaaaagatg gcaagccgat tttattcagc
     5821 cgattatgct ggattgacag gaaatattta tgaacggagt tgtgacaaaa gccttgtcgc
     5881 agctcctttt tctgaataaa cggtgtgcca gagatagcct atcggcaata aggcacgatt
     5941 ttgtaaaata tgggaagcga tttttcaaaa tcgcttctta tcgctcaagg ctgaacatct
     6001 ctgtcacaac ctctccacta aatggtgttt aaaagcttgc atctgcgata ataagaacaa
     6061 ccaaacgaaa aataggaagc actattttag tttagttgtt cttattactt gatgcaggac
     6121 agctttttca caacctcttt tttgtataga tgggcaaaat ttttgtaata ataggcgtat
     6181 gtctttttcg ttgaggagat gaaaaagcat atggcttatt ttttgtttgc tttgcttact
     6241 ttctttccac ttcaagtggc agaaatatct actttttatt atgtaagcgg aaccatatac
     6301 ttgaaatcag aaggagatca tttctatgaa aacaagaaca attgaaatac ttcacagaat
     6361 catgacagcc caatatccag taaaagcaaa acatctagca agtgagttca atgtcagtca
     6421 gcggacgatc cgtcaggaag ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc
     6481 ggagatccgt acaattcgca acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc
     6541 tcgattagaa actgaaataa aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat
     6601 ttttgatcta gttttagccg ttgcttacag ccgaatcccg atccatctaa acaaaaaaga
     6661 agaagaattt caaatctcta aaagtacatt agatgaagat atgagacggt tacgagcaac
     6721 actggtcagt tatgggatag agatcgtcag ttatggaaag caaggactgg tttataaagg
     6781 ttcagaacga tctatccgta cgatgatcta tgattttatc aatcagaatc tgggaatgac
     6841 cgacttcttt atgaatcaag atgcacaatt aacaccggct caaaaaatct ttctccatta
     6901 ttttcctttg gtggaaatca aaaaaataca ggagatctat tccaataagt tgttgaaaca
     6961 agaagatgat atttataaaa aacaaatcct gctttttaca ttgatctgga tccagcgaat
     7021 caggaaacat gaatttatct ctgccattaa ttggaaaaat acagaaataa aagacagtag
     7081 cttttctgca tttattgacc acatgatcga ggaatttcaa ttggacaatg tcccgcaagt
     7141 agaaagaaat tatcttcgtt tcactattga gacgttcaat gctagagata tctccaattc
     7201 aatcgagtgg gtacaagctc aattactaac gatccaattg atccaatttg tagagaacga
     7261 gacacacgta cctttccatc taaaggaaga agctttattc gaaaatctct ataagcacat
     7321 ggctgcactg attgtaagaa tcaagaatca tatccaagtc atgaatccat tgaaagaaaa
     7381 catccgaaca aattatttcc caatctaccg cgcagtcact cattttgttc ctacgatcga
     7441 agaagtaatt ggtggcaaga tactggagga cgagatagct tttcttgtca tccacttttc
     7501 gacgatcgcc agtgtaatca agcgagactt gaattatgtt tataaatcag tcgttgtctg
     7561 taaccatggg atggctactg gaaatctgtt agcagagaat ctgaaagaaa agtttcctca
     7621 gatcgagatt actgcagttt taagttcaaa agaaatcgaa ttagtagata aattggatgt
     7681 caatctggtt ttcagcacct ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga
     7741 tccgatttta actgagacga atatcttggt tatagaagac tttttgaaaa gaaatgaaga
     7801 actacagcgg ttagagccta acagtcatga atcgacggag ttattcactc aaataataga
     7861 tgtcatgaaa gaaagtggtg gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt
     7921 attcgaaaca aacaatttag aaatcaacaa aagggagatc cagcctatgt taaaagatat
     7981 tttgacggac agcaatatca tgatcaaaga aaaagccact acttgggagg aatcgattga
     8041 actagctgct agaccattgg ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat
     8101 gattcgtgca gtgaatgatt acggaccgta tatcgtgatt ggcaaacaca tggcactagc
     8161 ccatgcccga ccagaagatg gggtcaataa attaggagtc agcgtagcta cgattgagca
     8221 accaatcgat ttcggtaatc ctgaaatgga tccagtaaag attatttttt gtttggcagc
     8281 agtagactcc tactcgcatc tgaacatcat gaaagaacta attgaattga tcaacaacga
     8341 agagaagttg gatcgattga ttgaatgcac aaatatcgaa gatttcaaac aactgctttt
     8401 ttcagtagtt gagaaataat aaatgatacg ggggagaaca aagatgaaca aattgaacat
     8461 tttattcgta tgcggtgcag gtttaggaag tagttttgca gcacagatgt ctgctgagga
     8521 tgttttgaac aaattaggag tagaagccaa actggaccat tgtgatattt cttccgcagt
     8581 ttccatgaaa ccagatgtga tcatcacagc agaaaatttt cgttcgcagt tcgaaaagtt
     8641 cgcagttgat cctaaaacgg cgatgatcta tttgaaaaat attgtttcaa aaaatgaaat
     8701 tgaagaaaaa atcaccccag ttctgcaaga taagggagtt atctaaatac a
//
LOCUS       DANKHJ010000103         8608 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000103 DANKHJ010000000
VERSION     DANKHJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..494
                     /locus_tag="PW113_002684"
     CDS             <1..494
                     /locus_tag="PW113_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2526943.1"
                     /translation="MVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNTWLMK
                     GYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFMLAKFL
                     LRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGLTSGG
                     TKG"
     gene            507..1322
                     /locus_tag="PW113_002685"
     CDS             507..1322
                     /locus_tag="PW113_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2526944.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            1473..2639
                     /locus_tag="PW113_002686"
     CDS             1473..2639
                     /locus_tag="PW113_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2526945.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            2869..3516
                     /locus_tag="PW113_002687"
     CDS             2869..3516
                     /locus_tag="PW113_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2526946.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            3600..4544
                     /locus_tag="PW113_002688"
     CDS             3600..4544
                     /locus_tag="PW113_002688"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2526947.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            4679..6130
                     /locus_tag="PW113_002689"
     CDS             4679..6130
                     /locus_tag="PW113_002689"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2526948.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            6151..6987
                     /locus_tag="PW113_002690"
     CDS             6151..6987
                     /locus_tag="PW113_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2526949.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            6987..7646
                     /locus_tag="PW113_002691"
     CDS             6987..7646
                     /locus_tag="PW113_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2526950.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            7772..8461
                     /locus_tag="PW113_002692"
     CDS             7772..8461
                     /locus_tag="PW113_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526951.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            8467..>8608
                     /locus_tag="PW113_002693"
     CDS             8467..>8608
                     /locus_tag="PW113_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2526952.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIE"
BASE COUNT         2792 a         1515 c         1778 g         2523 t
ORIGIN      
        1 agatggtacc gactatggca gcattgactg ccttttatgt catggctttc ttattagaag
       61 cgctcgataa gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata
      121 cttggctgat gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac
      181 ttgatggggc agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga
      241 tgctcgccgt tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta
      301 agttcctgct tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt
      361 ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac
      421 cgatttgtct attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg
      481 gaacaaaagg ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat
      541 tacgtacgcc aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct
      601 tgatatttgt atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc
      661 agatccaagc gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag
      721 gaacccaaaa attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa
      781 cgatagctgt gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg
      841 atcatggaac ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat
      901 ctggtatacg ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta
      961 cgcccgaaga tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa
     1021 tcattttatc ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt
     1081 tattcggggc ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata
     1141 cattcaaaga gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag
     1201 cagttgtagg gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa
     1261 ctgtcttgct gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat
     1321 gacaagttta agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt
     1381 ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac
     1441 gtaatagggt agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat
     1501 tattttcacg ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac
     1561 aatacggaga tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa
     1621 tgaaagagat tacaagtaac gcatctttgg cactaggaga agcctatatg gatcatcgaa
     1681 tcgagatcga gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct
     1741 ttttacgagg aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa
     1801 aaaacaaaga agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc
     1861 tagatccaac tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac
     1921 aagcacaaat caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa
     1981 cgttgcttga tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata
     2041 atgtcaaggc aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa
     2101 taaaagaaga acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa
     2161 aaggcgaaac gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa
     2221 atcttgaagg ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga
     2281 tccatggaat cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt
     2341 tccctggcgg ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt
     2401 tgcaactgat tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga
     2461 caaagaattt tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc
     2521 gaatgtggga tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg
     2581 tgattcaata tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat
     2641 tgaggcaaaa gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag
     2701 aagtgaactt ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta
     2761 tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc
     2821 gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact
     2881 gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat
     2941 caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac
     3001 tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag tgaagcaagt
     3061 actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac
     3121 ccagatactg caattactag tttggaatta gatagtgatt ggggatctta cttctacaag
     3181 atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa
     3241 atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa
     3301 gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc
     3361 gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact
     3421 tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa
     3481 acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt
     3541 gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact
     3601 tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg
     3661 gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat
     3721 ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg
     3781 atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc
     3841 tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt
     3901 ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg
     3961 ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac
     4021 tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta
     4081 agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg
     4141 atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt
     4201 tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca
     4261 ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt
     4321 tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg
     4381 aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc
     4441 cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata
     4501 cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc
     4561 atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt
     4621 actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat
     4681 gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact
     4741 agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc
     4801 gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc
     4861 attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc
     4921 tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg
     4981 gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt
     5041 caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg
     5101 tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt
     5161 agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat
     5221 tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca
     5281 aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta
     5341 tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac
     5401 acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca
     5461 gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg
     5521 gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta
     5581 tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg
     5641 agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa
     5701 gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat
     5761 ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc
     5821 taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga
     5881 ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat
     5941 cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca
     6001 tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga
     6061 tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc
     6121 atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata
     6181 actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac
     6241 agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt
     6301 cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa
     6361 gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa
     6421 atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa
     6481 gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc
     6541 gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca
     6601 gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc
     6661 acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat
     6721 gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct
     6781 gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa
     6841 ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg
     6901 acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa
     6961 acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt
     7021 ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt
     7081 cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc
     7141 tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag
     7201 tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag
     7261 tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag
     7321 caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg
     7381 tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg
     7441 tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat
     7501 ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag
     7561 ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa
     7621 aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat
     7681 ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg
     7741 taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga
     7801 atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat
     7861 gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg
     7921 agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact
     7981 gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa
     8041 aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga
     8101 gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt
     8161 accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc
     8221 aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa
     8281 aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga
     8341 ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga
     8401 ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg
     8461 aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa
     8521 gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat
     8581 tccgctacgc ttaaaaatgt gatcgaat
//
LOCUS       DANKHJ010000104         8587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000104 DANKHJ010000000
VERSION     DANKHJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(46..1512)
                     /locus_tag="PW113_002694"
     CDS             complement(46..1512)
                     /locus_tag="PW113_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2526953.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1899..2495
                     /locus_tag="PW113_002695"
     CDS             1899..2495
                     /locus_tag="PW113_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2526954.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2553..3035)
                     /locus_tag="PW113_002696"
     CDS             complement(2553..3035)
                     /locus_tag="PW113_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526955.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3141..3338)
                     /locus_tag="PW113_002697"
     CDS             complement(3141..3338)
                     /locus_tag="PW113_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526956.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3633..4391
                     /locus_tag="PW113_002698"
     CDS             3633..4391
                     /locus_tag="PW113_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2526957.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4451..4879
                     /locus_tag="PW113_002699"
     CDS             4451..4879
                     /locus_tag="PW113_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526958.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4925..4997
                     /locus_tag="PW113_002700"
     tRNA            4925..4997
                     /locus_tag="PW113_002700"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4958..4960,aa:Gln,seq:ctg)
     gene            complement(5147..5518)
                     /locus_tag="PW113_002701"
     CDS             complement(5147..5518)
                     /locus_tag="PW113_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2526959.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5733..5918
                     /locus_tag="PW113_002702"
     CDS             5733..5918
                     /locus_tag="PW113_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526960.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6001..6189)
                     /locus_tag="PW113_002703"
     CDS             complement(6001..6189)
                     /locus_tag="PW113_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526961.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6213..6476)
                     /locus_tag="PW113_002704"
     CDS             complement(6213..6476)
                     /locus_tag="PW113_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526962.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6634..6891
                     /locus_tag="PW113_002705"
     CDS             6634..6891
                     /locus_tag="PW113_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2526963.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6962..7153)
                     /locus_tag="PW113_002706"
     CDS             complement(6962..7153)
                     /locus_tag="PW113_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526964.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7294..7614)
                     /locus_tag="PW113_002707"
     CDS             complement(7294..7614)
                     /locus_tag="PW113_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526965.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7637..8071)
                     /locus_tag="PW113_002708"
     CDS             complement(7637..8071)
                     /locus_tag="PW113_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2526966.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8406..>8587
                     /locus_tag="PW113_002709"
     CDS             8406..>8587
                     /locus_tag="PW113_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2526967.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1695 c         1361 g         2812 t
ORIGIN      
        1 caggaactgc tgcaaataag caattcctgt ttttatgttc attgattaat gaatagagcc
       61 cattagtttt ttgattggtt ctttcacagt aaatagcaag ataccaccga cgattgctac
      121 tgcaccgata ataccgaagt aagcagattc tgttcccggt gtgtagaagc gtgcaatttg
      181 tgcattgatt gcttgtgaag aagcatcagc taagaaccaa atagcgattg tttgtgattc
      241 aaatgctcgt ggtgctaatt ttgttgtcac agacaaacca actggtgaaa gacacaattc
      301 agcaataacc acgatgaaga aacttcccaa caaccacaat ggacttactt ttccacttgt
      361 accataaagc atacccggca acatcaatag caagtacgat aatcctgcaa ataaaagtcc
      421 aagagagaat ttcactactg tagatggttg acgttcacca agtttcgtcc acatcgttac
      481 gaatactggt gtcagaataa agatgaaaat tggattcaat gattgatacc agcttgcttg
      541 gatcgttaca ccaaatagcg tagattgtgt acgttcacta gcaaataaag ctaagataga
      601 tgaaccttgt tcttgtaatg accagaaaat gattgctgca aggaataatg ggatataagc
      661 ccatactctt ggtttttctt cagcagttac atcttttgaa gtcagcattt tgataaagta
      721 atacaacggt aagataatcc ccaatacact gattgtattg acaaaaaagt caattgtcag
      781 atgaccagtt agttgtgccc cgccgaatac tgcagcgata acaacaagtg cgatgatcaa
      841 gttacggatg aatcctttac gttcttcagt tgtcaaagga ttcggcggtg ttgtacccac
      901 tccgtctaat gtcttgcgtc cttggatgat gtattgtaat aatccaaaga acatcccaat
      961 agcagctaaa gagaacccta aatgatagtt atattcttga ccgattgtac caacgattag
     1021 tggtgctaat aatgccccta agttgacacc catgtaaaaa atcgaaaatc ctgcatctct
     1081 tcgcaagtca gtctttgaat aaagatgtcc gaccatgcca gaaacatttg gtttcaaaaa
     1141 ggcggttccg ataataatca atgccatcga aacaaacaat gtacttacac caaatggtaa
     1201 agccaagacg atgtggccaa gcataatgaa aactccaccg aagaatacag tgcgttttgc
     1261 tcctaacact cggtcagata cccatccgcc gacgatacta gacatgtaga tcaaagatcc
     1321 gtagatcgac atgatagcta atgctgttgc tcgtggtaat ccaagtcccc cgttggcaac
     1381 tgtatcgtac atataataga taagtaatgc acgcataccg taataactaa atcgttccca
     1441 catctcagtg aagaacaaag ttgacaatcc cttcggctgc ccgaagaagc ccttgtcttg
     1501 ctgtttttcc ataatctata ccccaattct ttaaattttc agaaaatatt atacatcttt
     1561 accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc tataaaagca
     1621 agtaattttt aatggcaata tcataaaaaa aagaagatag agatttaata attatatatc
     1681 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
     1741 aaaatcctta cataacgatt tttcttatta aaattgtttt ttgaacttcg cttatttttt
     1801 gtgagcatat ctgtttactt actttttgta aggtgctata cttcatttcg taatcaatta
     1861 aaaaaatgca aaacgcaaat caaaggagaa attttattat gacaactttt actgacactt
     1921 tgaaaaaccg ccgttcaatc tatcatctag gacgtaatgt ttcactttct aatgaagaat
     1981 taacaacttt gatcaaagaa gcaattaaag aaagcccaac tgctttcaat gctcaatcaa
     2041 cacgcgcagt tatccttttc ggagatgcac atgaaaaact ttgggaaatc actgaagaag
     2101 cacttcgccc attgactcca gcagaagctt tcccaaatac acaaaacaaa ttagctggtt
     2161 tcaaaaatgg ttatggtaca gtattattct tcaaagatac tgacgtggta aaaggtctac
     2221 aagaacaatt tgaattatat gcagacaact tcccagattg gtcagaacaa tcaaacggaa
     2281 tcgcaacagc taatacttgg gtagcattag ttgataaagg attgggcgca aacttgcaac
     2341 attacaaccc agttattgac gaagcagttg caaaagaatg gaatatccct tcaaactgga
     2401 aactacgttc gcaattagta ttcggttcac cagaaacacc ggctgaggaa aaagaataca
     2461 tgaacgatgc cgatcgcttc cgcgtatttg gataatttta tttgaaataa aaaacagagg
     2521 ttgtgacaaa catcttgtcg caacctctgt ttttattctg gatcaatcat aatatcaatc
     2581 tctgcctcat cgtttaagcg ttgggacaaa aatggaacta attcttctaa tgatgcagaa
     2641 aatttcagca tcggtcctcg ccatccgttt cggaaaaaat tcatttttac gattttagaa
     2701 ccttttggct gatataatac aattttattg atctcagaaa aaggaacacc ttttttgtca
     2761 aagctattca aaacaagtcc ttcttctgtg attcctctac tgtccaacaa aaaactaata
     2821 aaaatcattg ctgacaagat cccttttgcc aattcatctg ctgtaggatg actgatgagt
     2881 gtataccaaa tgactagtga cactaagaag ggtatcacca agtagaccca tcgatttcgc
     2941 acagtccgat aaataatttc ttttcgaacc agccaaagaa ataaaaacat cacaacaacg
     3001 aataaaaaca atagaaccgt aaagaatgtt accatgacaa ctcgcctcgc tttttcttct
     3061 ccagaaaata ggaaaagaat actgatttac cttctcaaag aaattggact tgcagcacgc
     3121 cgcacgtcca attgttttct ttattcaaat gtatcatatt ttgcaacaac catgcttgct
     3181 ttttcatcta ataattcttg gtcctctgca gcacattggg caactttgac cacggcgcag
     3241 tttgtcattt ttgatacaac ttcaccaatg accgtttttg taaaaccaat tggtttgcat
     3301 gcatattgtt ctcctaaatt aatcatttct tccgtcatca aaatcctcct aaactgctgg
     3361 aacttttctc cacattacca ctgctatttc tttggagatt taactcattg atttttattt
     3421 acagttaaaa atcaacctta caggcattat agcgtactgt tttcgttttc acaattaaaa
     3481 cagctgttgt atttagaaga atttttttca aaaaattaaa acatattcct taaaaactaa
     3541 ttatttccta catattaaaa tataatactt ccatttccaa taaaaataca tttataatag
     3601 gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc aaaaaactat
     3661 tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac ctacatatcc
     3721 attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga
     3781 caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt gattctcctt
     3841 ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt tatggttatg
     3901 aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc aatgcagatg
     3961 ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga gactgggcta
     4021 tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa acggattatc
     4081 acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta tctaaaggaa
     4141 atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact gattttatga
     4201 cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct tgtgtagtca
     4261 ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa aatcgagcag
     4321 taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg ctaactttgg
     4381 agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac ctttgaaggg
     4441 agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa ttccgattat
     4501 ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg agtggaacga
     4561 gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt
     4621 ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc taattgctcg
     4681 aacagagtat actcgctttg aatatattga acgtttagca aacatctatc atattatgaa
     4741 tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag aactgattgc
     4801 ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa agcaacaact
     4861 atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa
     4921 taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa ttgtaggttc
     4981 gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca
     5041 atctttttgc agggggatta ttgtattatt tttactactt gtatttagtt tacataatta
     5101 aattatgaat aataaaatcg ggcgtattct taatacaaaa actgatttac ccaataattg
     5161 ctaatgcttt ctccggaact ttttctctag gaacttcctc atacgtttct atgtgtgctc
     5221 ctttattgct aattttcaaa taatgatttt cttttaacac agaaagacca ttaaattcaa
     5281 ccgttcgttc tttcccattg gcatcagcag ctttctgcac ataccttgct gtaccatatt
     5341 catttacttc ctctggtttt tgtgtcttta catagacctc tcctttttca actaatggat
     5401 ttagctgatc aagtatacca gggatttctc cataacttcc ttcagtataa tatttcagtc
     5461 caaatagcaa aataccacta atcgccgcca gtccaatcaa atatttaatc actttcatct
     5521 ttctacctcc ttgttatata ttcagtatat gggtcagtta aagaaattcc atccgtccca
     5581 agttggagat ttttctccta ctttagaagc gagaatcgag tgttttttta gtaaaaatga
     5641 tcgttatttg atattattga cgtgtggata aatcagtttt tctcgttttg tccatgtaaa
     5701 aaatcatgtt gatagcaaag gatgtgttca aaatgaatca agaagaatta tttcaaaaag
     5761 tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa acgatttatc gaagaacata
     5821 aagaacaatt aggaccttat aaagagaaag cccaaaatct tttaaaagat gtaaatatcg
     5881 acagtgtcaa aaacaaattt aaaaatctat tcaaataagt aaaactttca cttttgtaaa
     5941 atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt tcttttctga atacttcata
     6001 ctaaaataaa cgtgtcggat cgtaatgata agtcacattg ccgttcagtg aggcttttcc
     6061 caccacatat ttacgattct ccaacttcag ccacgcttct cgaaaagcca ttaattcttc
     6121 tctttttaca tcaatctttt cgatttttcc atcgatcaaa tcttgaataa gttgttcata
     6181 tggattcatt tttttcctcc atctgctgtc aatcaataag ttgtaaaaat aggttcatat
     6241 gcttttttgg tatctagatc ttctctgact ttgactgtcg tataaataat tttttctact
     6301 gggatactat aataacttcc ttcaccatta ttgaatcggt gaacgtcttt ctttgctaaa
     6361 ataccattta ttttattcgt cagtacacgt tgttcgacac tgttacttac aacatagtaa
     6421 atcacccgaa tattattgat caaatgcaaa tcgatttcta acactacatg atccatgaaa
     6481 atcccactcc ttctttcact ttgattatag caagtttctc ctctaatttc aaaatcgata
     6541 cagattttac cggataaatc cacaaaactg cttgcgccat ttcgcacaaa gtgttatttt
     6601 gtatcatgga ctttatttat cggaggcaat agtatgatcg cgatcggtca atttgttttt
     6661 tatattcctt tttttattat gataagcatt cttttctatt acataaaatg gacaaaaaag
     6721 aagttttcag ttttactggc ctctttacct gctgtatatt ttacttatca aattttttct
     6781 tttaggcatt gggaaacaac gtctgtatta ataacacaca tcatcgaatt gacgctttca
     6841 gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg aatccctcgc
     6901 atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt aatcgttttt
     6961 tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct tgttttaatt
     7021 ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc ttttcgatca
     7081 ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc tgtaatactt
     7141 ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt tatttaagtt
     7201 atacgcatta ttctagtttc atcccaaata accttttttc atttttactc ctcgaactaa
     7261 tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt cattctgctc
     7321 ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat catagctgta
     7381 taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt tagctgtgac
     7441 agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac ctttttttcc
     7501 tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat
     7561 tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa ccattttcta
     7621 ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt gatacgacat
     7681 aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc gctcccataa
     7741 cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt cttggatctc
     7801 cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca ttgatttttt
     7861 tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa aaggcatagg
     7921 cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg gcttcattgt
     7981 cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg
     8041 ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc ttttattgta
     8101 acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga tgaacaaaag
     8161 caatttcttt caagttattt atacatttct tttctcatca gaacacctgt tctaatccca
     8221 ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac atcattctgt
     8281 tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga aatattttca
     8341 cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa taaaggagat
     8401 ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc ggcgctgcat
     8461 tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca ggtgatactc
     8521 tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc attgcagaat
     8581 caaattc
//
LOCUS       DANKHJ010000105         8493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000105 DANKHJ010000000
VERSION     DANKHJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..173)
                     /locus_tag="PW113_002710"
     CDS             complement(<1..173)
                     /locus_tag="PW113_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2526968.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PW113_002711"
     CDS             complement(593..1714)
                     /locus_tag="PW113_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2526969.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PW113_002712"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PW113_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2526970.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PW113_002713"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PW113_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2526971.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PW113_002714"
     CDS             complement(4304..5215)
                     /locus_tag="PW113_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2526972.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PW113_002715"
     CDS             complement(5232..6239)
                     /locus_tag="PW113_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2526973.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PW113_002716"
     CDS             complement(6236..8365)
                     /locus_tag="PW113_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2526974.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="PW113_002717"
     CDS             complement(8370..>8493)
                     /locus_tag="PW113_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2526975.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DANKHJ010000106         8412 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000106 DANKHJ010000000
VERSION     DANKHJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..208)
                     /locus_tag="PW113_002718"
     CDS             complement(<1..208)
                     /locus_tag="PW113_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526976.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDI"
     gene            complement(299..751)
                     /locus_tag="PW113_002719"
     CDS             complement(299..751)
                     /locus_tag="PW113_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2526977.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(764..1174)
                     /locus_tag="PW113_002720"
     CDS             complement(764..1174)
                     /locus_tag="PW113_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526978.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1449..1661
                     /locus_tag="PW113_002721"
     CDS             1449..1661
                     /locus_tag="PW113_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2526979.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1664..1876
                     /locus_tag="PW113_002722"
     CDS             1664..1876
                     /locus_tag="PW113_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526980.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2016..2171
                     /locus_tag="PW113_002723"
     CDS             2016..2171
                     /locus_tag="PW113_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526981.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2168..2392)
                     /locus_tag="PW113_002724"
     CDS             complement(2168..2392)
                     /locus_tag="PW113_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2526982.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2470..2658
                     /locus_tag="PW113_002725"
     CDS             2470..2658
                     /locus_tag="PW113_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526983.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2655..2828
                     /locus_tag="PW113_002726"
     CDS             2655..2828
                     /locus_tag="PW113_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526984.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2984..3319
                     /locus_tag="PW113_002727"
     CDS             2984..3319
                     /locus_tag="PW113_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526985.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3552..4493
                     /locus_tag="PW113_002728"
     CDS             3552..4493
                     /locus_tag="PW113_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL2526986.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4495..5385
                     /locus_tag="PW113_002729"
     CDS             4495..5385
                     /locus_tag="PW113_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL2526987.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5428..6228
                     /locus_tag="PW113_002730"
     CDS             5428..6228
                     /locus_tag="PW113_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2526988.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6243..7094
                     /locus_tag="PW113_002731"
     CDS             6243..7094
                     /locus_tag="PW113_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2526989.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7295..7756
                     /locus_tag="PW113_002732"
     CDS             7295..7756
                     /locus_tag="PW113_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2526990.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7782..8234
                     /locus_tag="PW113_002733"
     CDS             7782..8234
                     /locus_tag="PW113_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2526991.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
BASE COUNT         2975 a         1314 c         1720 g         2403 t
ORIGIN      
        1 taatatcatt ttttaacata gtaatttgtt tatttaaatc tgatagtgtt tcttccttac
       61 ttaatataaa tctttctcta tcatgtaatt tatttagttt ttcatcattt ttttgttcta
      121 attcataagc ttcttttttt attgtgttag ctttcgacag tttagtgatt cctataatta
      181 ataaaataag tccaatcaat aataacattc aaaactcctc atttctttga tataattatt
      241 gtagagttta ctcagaaatg aggaaagagt ccatgtgaaa gcatgggctt ttttattttt
      301 aatgttgtaa tactaaatgt tcaagattat gaggaagccc taaatattca ataatttgga
      361 actgactcat atcagataaa tcttgttcat ctattgttct agttaacatt tcaattgcaa
      421 acatattagc ttcagcttct ataccataaa tcatacaact aaggttgttt gctctaaaag
      481 ctgctgtgca aataccttta tggagtctaa cgtgggctat ttcatgccat aatacaaatc
      541 ttttcataga aggtgatagg ttttgattaa ttaatatcac ataacatctt ttattgtgta
      601 tcgtttgtcc taatgtttta ttccctaaat cagtttcaat taaatctata tcagaatatt
      661 tgattatttt atatggatta atcgcatcag atccaaactc tacaaataat ttttctattt
      721 cttctaatat ccagtatttg tttaaatgca taccaaagcc cacctattga cgatattttt
      781 taggagtgaa ttttttcttt gctttaactt tggttaaggc aagtgcaagt tgaatggcat
      841 cgtataattt ttccttatct tcatcgtcta aaggttcacc ataataattt gtttcagcat
      901 ctgaattcaa gccttcaagt aatttttcag cttgtaaagc aatatcttgt ttatcttttt
      961 ctgttaaatc ataataatgg cgtttatcag tacgtcctaa aagataatca gttgatacat
     1021 taaagtaatc tgcaactttt tgtattttat ctccagatgg agtactttta ttccatctag
     1081 acatgcttcc atttccgaaa tttaactttc tttcaagttc tgccaacgtc attgaacgtt
     1141 cttttaataa gatttttata cggtctaata gcataggata tgcattcctt tcaaaatacc
     1201 gacaaaaaaa gtatttttct gcaaattatc tatttacaaa tagagaattt gcagttatac
     1261 tagttttgta agcaagatat ttacggcata caaagactaa aagcaataaa ctaatagtct
     1321 ccccagacat cagtaagctc gctttttgtg cgctgtattt tgtatgctta cataatagca
     1381 tatttgcagt ttttgtcaaa cttattagac aaaaatatta gaaaatattc gataaggggg
     1441 tatgaagcat gactttaaaa cagcaagtat taattgcttt agtaaagaaa ggttggagtc
     1501 aaagagagtt agctcgacga atgggtatat caattactta tttacgagat atcttcatcg
     1561 aaaaaagaaa acctaaggaa cgtttaaaac aaatcgaaga acttttggat atcaagttag
     1621 aagtagattc taatgatcag ccatcagaac aggaggcgta aaaatggtac aacgtttgag
     1681 caaagcggag tttaaagaga aatataagta ttctgaaagt acttatcaaa gaagaatgaa
     1741 ggaatttaaa aaatcacgtt ttagtgaggg gtataacgca gttacttcca aagaaataac
     1801 aattgatgtc gatttatatg aacagtttag aacatggcgt tctaaaaatc gatttagagt
     1861 tagagctgtt tgttaatagc taaaaaatct aaattcacca cttctgaaaa gaggagttgc
     1921 tgctctcctt tcaagtttgc caaagtccac gttttttcat tttggcaact cctcttcttg
     1981 gaggtggtga cggcgagaga cgaaggaggg atgacatgaa ggaagaatca aaaataggga
     2041 ttttgaatga gaacaattac acaagattaa ttattgaaac agaagaaggg aaaaaagtag
     2101 cagaagttac attgaccgat gcaactcctg ctgacggtta tcgtattcga ttaactccta
     2161 aatatgatta accttttggt gggtgtggat cgtttccgtg actatcacgg ctttgaattt
     2221 ttccatctct gccatgaata aatagctcag atccttgatt tttagaaatc tctctagcga
     2281 tttttactgc ttctgatttt gtattagtat gtgcagtcgc tttagaattt ccagcacctt
     2341 tgacattcca accaccattg gaattaggta caacatgttg attttttccc atagttttca
     2401 cctccatttc tatagaaata ttttatcaaa tagaaatgga gaaattcaat aaacgaaagg
     2461 agaagtgata tggcatatac cttgcaacaa gaacatcaaa ttctcggttt gattaaacaa
     2521 cgcaggaaac aattacaaga tgaccgtgca gcgcttagaa aagccgatga gctatcagat
     2581 aggcaagctg aactaattgc ttctgaactt gaggatttga gaatgctaga aataaaaaat
     2641 agggagatta gattatgaag aagacagaca cactttttat aggattcatt ttggggttat
     2701 tagtgattgt agcgcaccaa agtattatcg ggggaagctt gttcgcagca ttgatggttt
     2761 taatcaatct gcttgattca aaagaaagga gcaactatgg cacgagaaga agcgctaaaa
     2821 atcggtaaag tgattgctga taatcggtgg ggaaatagcc gccctattat tttaagcaag
     2881 caacatatcg aaaagcaaaa agcatggcaa caaataaaaa agtgactctg ccagccagca
     2941 aagagtcaca tacaaattta taaaactagg agaattatat cacatgaata agaaaattga
     3001 aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa ggtgtttcta ttatttgtac
     3061 agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt gagacaacag aaattttact
     3121 ttgtcttgct atgcaagaag aacatttaga tgaaaatctc ccattgtcag cacatattat
     3181 gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat gaagaagaaa atcaaccttc
     3241 aaatcatact tttgttatta ataacaagga agatttagct gatgtgatga ctcgtattct
     3301 caagggggaa tttcaatgat gaatgaaaaa acaattgatc tactcattat tttatttttc
     3361 acgttagtga ttgtagcgaa aagttatgta atctttggct tgttattcgc aattaccatg
     3421 cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac atgaacgagt tagcaaatct
     3481 tgacaactat ttaactgatc ctgattatgc taaaccacca tatgaagcac caattgatga
     3541 ggaggatgaa gatgagtaaa tctaccttag aaatgagcca tcaagaatgg cttgaagacc
     3601 gtaagaaagg catcggaggt tcggatgttg gaacaatttt aggattgaat aaatggaaat
     3661 ctccttatca actatggcta gaaaaaacag gacaagtagt acttgaagaa tcaggaagtg
     3721 agcccgctta ttggggtaat gttttagaag aagtggttgc taaagagttt caggaacgta
     3781 caggcaaaaa agtacgcaga agaaaccaag tatttgaaca tccattacat ccatttttaa
     3841 gagcaaatat tgatcgggaa gtagtgggag aaaatgccat tctggaatgc aaaacagcta
     3901 atcaatttct cggtaaagaa tgggaaggcg aagaggtacc gcttagctat ctctgtcaag
     3961 ttcaacatta catgaacgtt ctaaacaaag actattgtta catcgctgtc ttaatcggtg
     4021 gtcaaaaatt tatctggaag cggattgaac gagatcaaga gctgatcgat acaatcactg
     4081 aacaattagt agagttttgg gaaacaaacg ttcttggagg tatcgagcca attattgatg
     4141 gtagtcaagc gacagctgat ttcttgaaag agaagtatgc agatgttgaa gacgttcaaa
     4201 cgacgttacc aattcatttc gatgaactag ttgaacagaa aaacgaactc aaacggacta
     4261 agaaagaaat tgaatcagct atccgtcaag tagacaacga gattatcagc gagctaggta
     4321 aacgtgaagc cagtatcggt atcactcaaa aaaacatcat cagttggaaa tttgtccgta
     4381 cgagacgtat gaactcgaag aaactagcag agaaatatcc agatgtcgca aatgatgaag
     4441 agatttataa cgttactgaa tcaagaaggc taaccgaaag ggagatcaaa taatatggca
     4501 acaaatgaat cgttaaaaaa tcaattggca gcaaagccac agaaacaagt tgcaccaggt
     4561 caattaggac ttaaagcttt gatgaataca ccaacaatga gaaagaagtt cgaagaggtg
     4621 cttcatgaca atgctaatgc ttttatgtcg aatgttatga ctcttgtatc taatgacagt
     4681 tatcttgcag atagtgaacc gatgtctatc atgagtggtg cgttaactgc tgcaacatta
     4741 aatcttgggc tagataagaa tttaggttat gcatatttag ttccattcaa tagtaaaaac
     4801 aagcaaacag gaaaatggga aaagaaagct caattcatgc ttggctataa aggatatatc
     4861 caattagccc aacgatcagg taaatacaaa gcattaaatg tgatcgaagt ttacgaagga
     4921 gaactaaaaa gctggaaccg actgacagaa gagtttgagt ttgatccaaa tggtagaacg
     4981 tctgatgaag tcattggata tgttggctat ttcgagttac tgaatggatt caaaaaaact
     5041 gtctattgga ccaaacaaga aatcgaagct catcggattg ctaacaataa agatcgcgat
     5101 aagacaaagt taagtggtgt gtgggcatct gattacaatg caatggcacg aaaaactgtt
     5161 ttgagaaatc ttctttctaa atggggaatc ttgtccatcg aaatgcaaga agccaccaca
     5221 tcggatgaga gagtccaaag ggttcaagaa gacggcagca ttattgctga aacagaagtt
     5281 gaagaagata ttcctgaaag aaaagaagca gaggttatat ctgaagaaaa tgaagatgta
     5341 caaactggat tatttgatgc atctaatccg ccgttaaaca aataatgagg gagctttctc
     5401 ccttgctttt ctagaggaga aatacgaatg aacacaggat atgtcaaatt acatcgaaaa
     5461 gtgatgaatt cattcgtttg gaccaatcct tatatgtata aattatggaa cttgtgtttg
     5521 atgaaagcga gtcatgagaa tcgcaagata ctttttaatg gaaaagagat acaagtgaac
     5581 agcggagaat tcgtcacagg gcgcgatgct atcacatctg agatgaacga aggcgtgaaa
     5641 cgtgaacatc aagtgaacag cggttctgta tggagatggc taaaacaatt tgaaaaaaac
     5701 ggaatgttga acatcaaatc aactacgaaa tacagcgtta tatcaataaa aaactggtct
     5761 ttgtaccaaa gcagtgaaca acaaatgaac atcaaacgca caacaagtga acaacaagtg
     5821 cacacaaaca agaatgaaaa gaattataag aatgaaaaga ataataaaac atcatcgtcg
     5881 caaccacgca aaaagcgtgt ttacgacacc gactcagttt actacattct cgcggaggag
     5941 ttattcaaac agatttgtca gaatcaggaa atcaaaaagc cgaatctgca aagttgggca
     6001 gataacattc ggaaaatgat cgagatcgac aagcggaccg aaaaccaggt acggggaatg
     6061 attgaatgga gtcagcacaa tgtgttttgg gcatcgaata ttttatctgc taaaaaattg
     6121 cgagaaaaat acgacacaat ggcagcgcaa gccaatcgtg attataaaac aaaacaaact
     6181 aaaacgcttg aatacgagaa atttagcaca gatgagttgc ctatttgaga ggaggcgtag
     6241 gcatggaaac tgttggggaa atcatggaaa agctgataca gaaagtgctt gtccaacgtg
     6301 gcgaatgtcc tgaatgcgga cagcctttgt atggatggcg tacgaagaac cctgatggct
     6361 cagaacgttg taagccaacc tgtatgcaat gtgggtataa agcattgcga gttcaggaag
     6421 acttacaaac agaacgaatt tacaacgaga gcctgaaagc aagagcaatc aattttttca
     6481 aaggtggttc tgttgtgcct aatcaagcgt tgtttgattg cacattgcag aattatcaaa
     6541 ttgtcgatca agaaacaaga caagcggttg aagtaaccaa acgctttgtt aattcagtct
     6601 tgttaggaaa tccaagtcac cttgttttaa ctggaaaaca aggaacaggt aaaagtcacc
     6661 tagcgatggc agcggcttgg gaagtcttga agcgatcaaa ctacgataaa aaaatcttat
     6721 ttatcgggtt acaggaaatg ctggatcaaa tcaaattttc gtacaacaat cctgaactca
     6781 gaaaaacgat tgagggatcg ttgattgcag atatcaaaac agcggattta gtcatcattg
     6841 atgatatcgg ttcagaacta ggaaaagatg catcagatag tcgagcgttt ggcataaaca
     6901 cgctaaattc gttcttggat gcacgacaga acctagcaac gattatcaca acgaacttgc
     6961 ttggtgaaga actgaaaaaa gcttacggta cgagaacaat atcaagaatg tttgtcaact
     7021 ctgatggatt tacgatggta ttttctcaaa cagcagacaa gcgcataaaa ccagtcaaag
     7081 gagcgatcgc atgaatttga ctaaacaaga aaaaagaatc tgtcgtcaga taatgttgca
     7141 aattataggt gatcgtgaca tggacgaatt atctcaacaa gagagtcgaa tggttcggaa
     7201 gataagtaag ctaattattt atcgggatac agaaagattt ctcactgttt taggtaacgt
     7261 ctacgagaat ccagagttat tggaggtaga gtaattgcca aaaatattag atgcatgttg
     7321 cggtagtaga ttgttctggt ttgataagaa caattctgac gtaacattta tggattgtcg
     7381 tcagcagtat gaagaattat caacaggtca tgtgatcaat gttgatccag atgtggtcgc
     7441 agactttcgc gatatgcctt ttgaagatga tgtgttcgat atggttgtat ttgacccgcc
     7501 acacttaaga catgtcggcg aaaactcatg gcttgccaaa aaatatggaa aattagacga
     7561 actttggcca gaagacatca ggcaaggttt tgcagagtgt atgcgagttt tacgaccttc
     7621 aggaacatta atttttaagt ggaacgagga acagatacca ctttctgatg tgttagaggc
     7681 tattggagaa cagccattgt ttggtaataa acgaagcaaa acacattggc ttgtgtttat
     7741 gaaaaaattc agctaacgga ggaaacaaag gaggaaaagt aatgaagatt gtattaaata
     7801 aatgttttgg tggctttggg ttgagccatg aagcaaaaat ggaaattttt aaaagaaaaa
     7861 atattgaagt attcccatac atcaataact ttagctatga ctcagatgat gaatacacga
     7921 gatacacagg ccaaaagcta ggttcgatgg attttatcta ctatttcaaa aaagacccga
     7981 aaatcgacaa agttacagga atttatagcg aaattgaccg actgtacgga attgctgatg
     8041 attccagttt ttcaagtgat tcaaacagag gtgataagga cctcgttgct gttgtcgaaa
     8101 aactaggaag tgaggccagt ggaccatatg caagtcttaa agtcgttgat atacccgatg
     8161 gggctgagtg ggaaataagt gattatgacg gcgttgaaac tgcacattat ggttttcaaa
     8221 caggtagtat ttagtccact aatgacaact aaagaggagg cagaaggatg agtgaagatg
     8281 aatactttga acagtagtct attaacgaca gagattttta actggaaagg ggtgtagtca
     8341 tggacgcatc ggaagtagta aataaaatta atagtctcaa agaggttttc ggagacgtcg
     8401 aagtagtgat aa
//
LOCUS       DANKHJ010000107         8231 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000107 DANKHJ010000000
VERSION     DANKHJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..518)
                     /locus_tag="PW113_002734"
     CDS             complement(<1..518)
                     /locus_tag="PW113_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2526992.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSE"
     gene            complement(567..1070)
                     /gene="trmL"
                     /locus_tag="PW113_002735"
     CDS             complement(567..1070)
                     /gene="trmL"
                     /locus_tag="PW113_002735"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2526993.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(1291..2157)
                     /locus_tag="PW113_002736"
     CDS             complement(1291..2157)
                     /locus_tag="PW113_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2526994.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            2266..2907
                     /locus_tag="PW113_002737"
     CDS             2266..2907
                     /locus_tag="PW113_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2526995.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(2969..4330)
                     /gene="mgtE"
                     /locus_tag="PW113_002738"
     CDS             complement(2969..4330)
                     /gene="mgtE"
                     /locus_tag="PW113_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2526996.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(4348..5247)
                     /locus_tag="PW113_002739"
     CDS             complement(4348..5247)
                     /locus_tag="PW113_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2526997.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(5249..6046)
                     /locus_tag="PW113_002740"
     CDS             complement(5249..6046)
                     /locus_tag="PW113_002740"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2526998.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(6024..6728)
                     /locus_tag="PW113_002741"
     CDS             complement(6024..6728)
                     /locus_tag="PW113_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2526999.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            6859..7434
                     /locus_tag="PW113_002742"
     CDS             6859..7434
                     /locus_tag="PW113_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2527000.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            7552..8229
                     /locus_tag="PW113_002743"
     CDS             7552..8229
                     /locus_tag="PW113_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2527001.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         2432 a         1769 c         1478 g         2552 t
ORIGIN      
        1 aactcactta gctcttttcc tgtcatccat tgaatggctg cagttaaaga agctccatgt
       61 cctacgaata aaagtgggcc atctttatgt cgggcaaccg catctgctac aacagtttcg
      121 atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag gagaaggatc atacagatct
      181 aaatgattac gcaaattcgg cagattatcc ggatatttct ggtacatttc atcgatcgac
      241 tgaccttcta aagtacccag ccctagttct cttagcttat ctgtataaaa aatctctacc
      301 ggacgagcaa gctctttgtt gataccttct gctgtatcac gtgctcgctt tgctgtactg
      361 gaataaattg ctttaaaagg aacatatttt acctttttcc caaatgcttt gatttcttcg
      421 taactttgtg gcaaaagcgg agaatcgcca ttgctccctt gaaaacgtct ttcctgattc
      481 cattgtgtct tcccatgtcg ggtaaaatat agttccatac gcagcaaact cctgtctttc
      541 ttttatttta ctctataata aactgtctaa tccagcttgt cattttcata gtgatgcgtc
      601 aattctaaat tagggaatcc ttgctggcga agtgcttcat acactatcat tgcagctgta
      661 ttcgataaat tcaatgagcg gacatgctca tcattcatag ggatgcgtaa acacttttct
      721 tcgttttcac gcataaatgt ttctggcaaa ccggttgttt cttttccaaa taaaaagaag
      781 tgctcttctc catcagcaaa attttcgtca ctatatactt tatgggcaaa ttttgagatc
      841 aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt ctaaattact gtggtaagta
      901 atatttacat cattccaata atccaaccct gcccgtttta gatgtttgtc atcagttgaa
      961 aaacctagtg gctcgattaa atgaagcgga gtattcgtgg ctgcacacgt acgggcaata
     1021 tttccagtgt ttgcagggat taatggttca aataaaacga tatgattcat caggatcatt
     1081 cctctcatat ttatgttcgt ttctattttc tgacaatttg atttttgcat aaaaaaaaac
     1141 gtatcaactc ggtgtcagtc actgcgagtc gttacgttag tgaagctctt ctcactaact
     1201 tttatcagaa caggaacctg ataaaaacca atgaaaaaac aacttgttgc taatataaca
     1261 ccctcaactt ttttttgcaa cctctactca ttaaaaactg ctagaaaaac gttttctctt
     1321 agttcctttt aataattcta tgtcaattgt aatcttcttc aaaggattct tatataagtc
     1381 tgctttgatt cgctcttcta cctgccattc taaaggtgac atggccaaaa gatctttttg
     1441 attttcctct ggtatctcaa attcataggt tacacgctga cgttcagtta atttcattgc
     1501 ttcagcaaac ttggctacta cacgttcatt ggaataactc tgtttttcag gttgattcgg
     1561 ataaaaagcc tgacgtagtt cctttaaata attttcttgt ggcaccacct tcaacacttc
     1621 tccatcttct gagagtacac gttggaattc ttggtaatga gaaggcgaaa agatatttaa
     1681 aaccgcgttg aatgtttgat cagcaaacgg caaattggtc aaatcagcta cacaccaaaa
     1741 tgcttcggca gattgttctg tagctgcgta gactccttct tttgaaatat caaatccaac
     1801 agcattttga atctttttca attttgaaag ttcattcaag aagctacctt ctccgcagcc
     1861 aacatctaat agcgagactt cttcaggtaa atgctgcgca atagtctcaa taacgggctg
     1921 atacatgcca gattggatca ttctacgcct tggaacgaac attccttgat cgtattctgt
     1981 tttaaccgca tgggataaaa aataaagtgt tccttttttg gaaaggtcaa aacggtgatt
     2041 atttttgcat atcaagccat tatcagactt ttttacttct tcttggcaaa ccggacagcg
     2101 gaacagctga tgattttctg ccaaaaactg gcgtcctcgg tcaatttttt ttaacatagt
     2161 tccccctcat ttctgtttta tcttaccgtt agatgctcac tttggcaact gctaaaaata
     2221 tgatacaatg cctagaagaa agtgaaagat aaaggagaag attagatgat caaagaattt
     2281 tgcgctgaaa actacacatc tattcctcta gcaattgcta gaggagccga tcggatcgaa
     2341 ttgtgtgaca acttagcggt cggtggcaca accccgagta ctggtgtgat cgaagaaacg
     2401 ttaagttatg ctggggaaaa agatatcccc gtaatgacga ttattcgtcc tagagggggc
     2461 gacttcgtct ataacgatat cgaattgaaa atcatgcata ctgatttgat tgaagcaaag
     2521 aaattaggga cggatggcgt agtatttggt tgtcttactc cttcaggctg gctagacgaa
     2581 gaggccttag aactattgat cgaaacagca gaagggttgc agatcacctt ccatatggct
     2641 tttgacagta ttcctgaaga tcggcaattc gaagcaatcg attggctggt agaacatggt
     2701 gttgaccgta tcttgactca tggcggtcct gctgacaaaa ctattgtaga aaatttccca
     2761 cggcttaaag aattgattgc ctatacagat ggccgcttaa tcattttgcc tggtggtggt
     2821 gtcactgcag agaatgcagc acatgtagct aaagaattga acgtaagcga agtacacgga
     2881 accaaaatcg tggatcttca gttataatct tataaacaaa aaaacatttg caacaggcat
     2941 aatgcttatt gcaaatgttt ttgttttttt acataaaata gcccataaat aaagaagcaa
     3001 tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt cgtgataaag ggaccgcttg
     3061 ctactgctgg atcaaatcct agacgatcca ttagcatcgg gattaaacta cctgcaagat
     3121 tcgccactgt gattgcaaat agcatagcca tccctatgac aaaccccaag ataaaattat
     3181 gtttccaaaa acctacgatt agaaaaatcg tgatccccgt aacagctcct gtgacaacgc
     3241 cggtcaagac ttcagccaaa acaagtcgaa caaatccatg atctttctca tcagaaatcg
     3301 caagtcgacg aacagccaca gctagcgact gggttccagc attaccggca gttcctgtaa
     3361 tcagcgaaat gaagactgct aaaatactcg cttcacttac caagtcttca tagtgactga
     3421 ttaaagaagc ggtcgacata cctaaaaaca acagagtgat cagccaaggc aaacgtcgag
     3481 acgctgcctt tactggattt tcattaattt cttccacatt gaccccggca agtcctgaat
     3541 aatcgctagc agcttcatca tcaataacgt cgatgatatc atcgactgtg acgattccta
     3601 acaaatgatc atcataatcg gtaacaggaa gagctaagaa gtcataatct ctaaatgttt
     3661 gtgccacatc ttcttggtcg tcgcctacat gaactgaaag cactcgttcg ctcatcagat
     3721 cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg cagagaaata acccctacta
     3781 gctgaccttc ttgattaacg acatagatgt aatagattgt ttcagctaca tctgcttcgt
     3841 tcttcagcac gtacatcgca gagcgaacag tttgattggc tacgatcgaa acaaattcag
     3901 ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag aagttccttg atttcactgg
     3961 catcgtctgc cgacatcaaa cttaaatatt tagcaatttg ctttttatcc agcgtattaa
     4021 gcaagtcaac agcattatct gtatacatct cagaaagcat atcagctgca tagctaggac
     4081 gcatttcatc taggtattct ttcatatgtt cgttgtcttc ttcaatcaca tcgaacatat
     4141 ccgctaattc tttaggcgac agataggaat agatcagctg cctgttctct tctgtaagag
     4201 attggtagaa ttgcccctgc tcatagatat gcatctctaa aaaactgttt ctgaatgcct
     4261 gaatattatt ttcccttaaa tcttgagtca actgggcaaa tctttcctct aattcttgat
     4321 tttcatccaa accaatctca cctcgcctta ttcttcaaac cgtctcacaa ctgcctccat
     4381 gtctgcggct aaaggctgct tgaactccag caactcttct gtaaatggat ggtaaaaacg
     4441 cagatcataa caatgaaggg cttgtctttt tattcccaga tccattcgcc cgccatacat
     4501 atcatcaccg agcagggcac agccaattgc ggaaaaatga actcttattt gatgtgtcct
     4561 tcctgtatgc agctggatat caactaacgc gatttctgtt agtcgcttct ctatccagta
     4621 ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc ttcaatagcg aagaataatc
     4681 tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa tctgaaagcc ttccactgac
     4741 taaggcctga tattttttca caaacttctt ttcacgaagt tgcatatcta attttgcgtg
     4801 cgcaaatcca tgctttgcaa aaagcatcaa gccagatgta tcccgatcta gtcgcgtcac
     4861 cacatggatt acttggtttt cgtaaccttg ttttttataa taagccttta cacgattagc
     4921 cattgtccca tttggatggt attgggcagg aatagacgaa acgcctgctg gtttatttac
     4981 taccagaacg tgttcatctt catacacgat atcgatcggt gtttcatcca aaagtacggt
     5041 ttcatgttct ccttcagcag gaatcacaat ggttacctta tcatccttag ctagagaaaa
     5101 caagacattt tctacttgat cattaacttt gatttgaccg ccttgaaatt ttattttcgc
     5161 taacagaccc tttgagatcc ctttttcttt taaaaaatat tttacttgct gagaatgatc
     5221 tttctcataa tcccatgtaa actgcatatc aatcctcact aataaaggca tctttcacac
     5281 gatgccaaaa atgcatatgt cgataagaag caaaatggat ccgttcttct gctattcgat
     5341 aataaattga gcggatattt gcttttgcga cattcaattg atcaaccgtt acaagataat
     5401 cttctgtatt ttccaatttt atttctagcc attcatcatg ggcaatcacg atcggagaac
     5461 ccaaagtacg aaacactcga ttattcagtg aagcaatctc tgccaactga aaggcattga
     5521 tactaggatg gatcacagca ccgccgacac ttttattata tgctgtcgaa ccagtaggcg
     5581 tagaaatcgt caatccatct cctctaaaac tttcaaacag ctcgtttttg atgtatacat
     5641 cggctaccat cgtacgattg gcccgtttaa tcgttgattc attcagtgct aaaaaatgct
     5701 ggtccggtgt ttcatccaaa taactgatcc gaacatccag caagggataa ctaacacttt
     5761 gttcccggtt cgttcgtaag ctgtctacta gttcttccag ctcgtaatcc cgccaatccg
     5821 tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg cacatcattc agacaatgac
     5881 tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc gactgagatg accaattctg
     5941 gctcatgatc atctcgttga atccctgctt gttctaaaag tgcgttcaaa cgttctgtca
     6001 cttctatagt ctttgcttct ttattatgaa cgatcgctac tttcatcccc tgcctcctct
     6061 ctttcgcttt ctttcttcaa aagcgttcga tagtagacat catctgattt tcctcttcca
     6121 tgggagaata accgctgtgc ttcttggatt tcttcacgga tactggacat ctcttcatcc
     6181 aactgatagg cagcttctgc cgcacgctga agtcgttcac tcatttcttc tggaaaaacg
     6241 ccttgatatt tataattcag cgaatgctca atcgttgccc agaaattcat cgcaagagtc
     6301 cgaatctgga tttccgccaa aattttcttc tcaccgctga tcagctgtac aggatactcg
     6361 atcaccagat gataagaacg gtagccactt gcttttttat ttgtgatgta atctcgttct
     6421 tggagaactt tcatgtcttt tcttttacgg atgatctcaa caacttgatg gatatcttct
     6481 acaaactgac acatgatcct taacccggca atatcagaca tttcttcttc taaacgtccc
     6541 attggaatat gccgtaattg tgattttgtt acaatactat cgactggctt gactctccca
     6601 gtgacaaatt caatcgggac atgtcgattt tgttcacgga actgtttgcg gatcccccgc
     6661 agtttcactt tcaattctga tacagtttgt tcatatggtg ctaaaaatgc ttcccaatct
     6721 cgttccatag ctgatacctc tacttcatta acattcattg tttattctac catatatgca
     6781 aagttttttc gtactcttga tttatgcatt gaccaaaaaa agaaaattag gcaaaataga
     6841 caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg aatataaatc tatgctgaca
     6901 aagcaagagt acgagatatt catcgctcac tatcaattaa cagataaaga tttccgtgta
     6961 cagactaata tttatttcga taccgttgat gaacagctga agaaacaaaa ttgtggccta
     7021 cggatccgtt tcgttggtca tcaagctgaa tatacattaa aaacacctgc tgaaaaaggg
     7081 cgtctcgaaa caacggatac ctttataaca gaagaagcag aagcatttat cttcgaaaaa
     7141 cgtctgcctc gttcaggcgc agtttttcaa aaactttctg atttgaatat cgatcctgct
     7201 tcattaatcc aaaccggaaa gcttacaaca aaacgagctg aattccctat tgaagaagga
     7261 cttttagcta ttgatgaaag ctggggagac aatctacacg attttgaact ggaactagag
     7321 gtaggggatg catctgtggg aaaaattgct tttataaatt ttttaaaacg tttttcttta
     7381 ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg aagctaaaaa ttagaaacct
     7441 ttcattttca caaggcactt gagttcattt ttttactttt ttctgttaag ttataggatg
     7501 tgaaagaatc tagtcactag ttgtatagaa tctattttgg gggagattca tatgatcgaa
     7561 atctatttat ttgttaatcc tttaggggaa tactgtttcg aaatggaaca acagttatta
     7621 caatttatcg aagaagagta cgggacacca tcaaaagaaa agatgcaatt tcgctttctt
     7681 cctttggtca atttgcagac aattggggat gtcatgcaac aaaaaggaat ttctcagaac
     7741 aatcttgagg aacgtaatca cttattctca atgacttatt ctgcagcatt ggactgcaaa
     7801 gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt tgatcaaatt gcaagaagca
     7861 gtcggctgta atgacatacc atattctcaa gaacttgctg aatccatatt tgaagaaatc
     7921 ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg acttcgttaa agaagtattc
     7981 ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa aacacccttc tgcagtcgtt
     8041 tacaattatg cctgcgaccg tgattttggt gttttagtag aagtaaaagg tacaagtagc
     8101 ctttggagaa tcacagagct gtgtaaaaca acagataact catatcaagt ttttaatggt
     8161 gaacatttac atacaaatga ttctcagttc cattcaagaa ctagccattt acacttgctt
     8221 tctaattaaa a
//
LOCUS       DANKHJ010000108         8228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000108 DANKHJ010000000
VERSION     DANKHJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="PW113_002744"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="PW113_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2527002.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="PW113_002745"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="PW113_002745"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2527003.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="PW113_002746"
     CDS             2591..3376
                     /locus_tag="PW113_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2527004.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="PW113_002747"
     CDS             3389..4240
                     /locus_tag="PW113_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2527005.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="PW113_002748"
     CDS             complement(4364..4816)
                     /locus_tag="PW113_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527006.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="PW113_002749"
     CDS             complement(5281..5649)
                     /locus_tag="PW113_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2527007.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="PW113_002750"
     CDS             5799..6281
                     /locus_tag="PW113_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2527008.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="PW113_002751"
     CDS             6318..7328
                     /locus_tag="PW113_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2527009.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="PW113_002752"
     CDS             complement(7443..8156)
                     /locus_tag="PW113_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527010.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DANKHJ010000109         8143 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000109 DANKHJ010000000
VERSION     DANKHJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..989)
                     /locus_tag="PW113_002753"
     CDS             complement(<1..989)
                     /locus_tag="PW113_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2527011.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLE"
     gene            complement(1001..1990)
                     /gene="galE"
                     /locus_tag="PW113_002754"
     CDS             complement(1001..1990)
                     /gene="galE"
                     /locus_tag="PW113_002754"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2527012.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            2239..2406
                     /locus_tag="PW113_002755"
     CDS             2239..2406
                     /locus_tag="PW113_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2527013.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(2468..4279)
                     /locus_tag="PW113_002756"
     CDS             complement(2468..4279)
                     /locus_tag="PW113_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2527014.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(4276..4641)
                     /locus_tag="PW113_002757"
     CDS             complement(4276..4641)
                     /locus_tag="PW113_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2527015.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(4804..5199)
                     /locus_tag="PW113_002758"
     CDS             complement(4804..5199)
                     /locus_tag="PW113_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2527016.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(5217..6179)
                     /locus_tag="PW113_002759"
     CDS             complement(5217..6179)
                     /locus_tag="PW113_002759"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2527017.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(6179..6391)
                     /locus_tag="PW113_002760"
     CDS             complement(6179..6391)
                     /locus_tag="PW113_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2527018.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(6412..7110)
                     /locus_tag="PW113_002761"
     CDS             complement(6412..7110)
                     /locus_tag="PW113_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2527019.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(7130..7672)
                     /locus_tag="PW113_002762"
     CDS             complement(7130..7672)
                     /locus_tag="PW113_002762"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2527020.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(7804..>8143)
                     /locus_tag="PW113_002763"
     CDS             complement(7804..>8143)
                     /locus_tag="PW113_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2527021.1"
                     /translation="RAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHA
                     AKLVVRKENNWTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYAL
                     FIRQKHALNS"
BASE COUNT         2361 a         1782 c         1382 g         2618 t
ORIGIN      
        1 ttttccaata ctgagttagc tgcttcaatc aacaattttt tctctgttga ttgcaaacgg
       61 atcacagaca tcggccattt cacgactcct gcgatgactt gcggataatc tgctagtttg
      121 ataagttttt ctatcggtgc ttttgccata ggaaattcat gacgtcctgc ctgataatga
      181 tcatgagaaa gaatcgatcc tccaacaatc ggcaagtcag cattcgaacc aacaaagtaa
      241 tgaggcaaga cttcagtaat ccgaagcagt ctagcaaatg tttcctttgt gattttcatc
      301 gggcggtgtt gttctgataa aatgatcgaa tgttcattgt aataagcata aggagaatat
      361 tggaatcccc agctttcacc gtctagattc attcgaataa tccggtgatt ggtgcgggct
      421 ggatagttca agcgacctct atatccttcg ttttccatgc aaagcatgca ggtaggatag
      481 tctactttta ccgcctcgcg ttctgctgcg atttgttttg gatctttttc tggcttagat
      541 aaattgattg tgatttccag ttcaccatac tccgactctg ccggaaattt gatgtttttt
      601 gcgatggctc ttgttttgat gtagtcattt ttcttactta aattaaagaa atactcagtt
      661 gcttcctctg ggcttttcga atagtgctgt gcaaagaatg cattgacgac tgaaggtggc
      721 ggtgtgagaa aatccattaa ttgcgcttca aaaatctctt gttctgctgg caattctgaa
      781 agaacaccat tttctcttgc cttagcgaca agctgatcca ataagtctac agattcttcc
      841 ttaactggtc ttacttcaat gctttctaga gcatcttctc ctaccatccc taaaagacga
      901 ttctgtaaat ataagcggtc catttccatc catccgccag cttcgatcgc aagtgtggca
      961 aaatcacaaa tcgtttgact gattgacata tctcattccc ctattcttca taaccatttg
     1021 gatggctgac atgccaatcc catgctgttt tgataattgc tttgatatct gtgtattcag
     1081 gtttccaacc cagaacctct ctcgcttttt cacttgaagc gactaatcgg ctaggatctc
     1141 cagctcttct tggtgctatt tctgctggaa tttcttttcc tgtcacttct ctagctgctt
     1201 ctagcatttc cttgactgag tatcctttgc tgctgcctaa attgaagaag ttgctttcat
     1261 ttccttcttt caagtattcc aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa
     1321 cataatctct gatacatgtt ccatctggtg tatcataatc gtctccataa acagccaatg
     1381 cttttctttg accaagagct acttgtaaaa tgattgggac aagatgcgtt tctggtgtat
     1441 gatcttctcc gatagatgca tctgcttttg ctccggctac attgaagtag cgcaaagcga
     1501 catagcgcat gccatatgct tggtcacacc acttcatcat tttttccatc atcaatttac
     1561 tttcaccata cggattttta ggattagctg gtgtatcttc tgtgattgga gactcttttg
     1621 gttctccgta agttgctgca gtagaagaga acacgatttt attcacattg aactcgtgca
     1681 tgacttcaag taagatttgc ataccgtata cgttgttgtt gaaatacatc aatggttttt
     1741 cgacagattc tcctactaaa gagcttgcag caaaatggat tactccttca atcggttcct
     1801 tttcaaatac ggaacgtaga aattctttat cccggatgtc tccttcataa aaatgagcat
     1861 ctggatgtac tgcttgtttg tgacctgtga gcaaattatc taccacagct acttgatacc
     1921 ccttttgaac tagctggtct acagcgtgcg aaccgatata accggctccg cctagtacta
     1981 aaattgtcat aaaaaacact cctttgatta gtaaaattgt gtttgttgat tatatataat
     2041 ttaaaaaaac gaagtttact tttatggtga aaacaaacaa agtaaaattt cgtaaattca
     2101 tatggtaaat ttataataaa acatttagta aacattttca aggatttttt gcaaaggttt
     2161 acaaacaaac gtaaaactag cctttttttc tcttttgttc cataatagat ataacaaata
     2221 ataaatgagg tggattcaat gaaaaaattt gtatctggaa tattagtagg aagtctggca
     2281 acagccgctg ccgttgctgg tttggtcgct tctgtgaaaa aaacagtgat cgatccaatt
     2341 gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag caatgcgcaa acgcgtttct
     2401 cgttaataat tatttaaaaa cacgcatgaa gacttgtgaa agaacagata agtcatcatg
     2461 cgtattttta tttgtcagtc aatataagtt gtgctatttt atctgcctgt ctcagattcc
     2521 atggatctgt ataaggtcta gctactgctg cagttagcca atccacacct tcaacaggta
     2581 ttccatagca ataaaagtca gactggattt cccctgaagc atcaatcacg cgattcgtac
     2641 ctcgctcaac atctatagct tctgaatgat aacttcctgt attatctgaa aatgaaaaag
     2701 ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg attacttgaa cgagaaaagc
     2761 ttgtttgtgg caatcgagct tcaatcaaat agcgactgct gatttcttta tcttgtagag
     2821 aaataacata gcggttgtgc ttttgctgaa tatcaaatgg cggttcgaac aaatgaaaaa
     2881 tacccgcatc tatcagtgct gctaattctt tcgtccgaat gactggcgga ccgatcgtta
     2941 aaaagctatt tagatgagta aaccatcccc atagtatttc tttgtattcc tttgcagaaa
     3001 aaacgtgcca ttcaatagcc tgccggattg gatcacgcca gtcttttaat gcatcaaatg
     3061 ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc ttttatctgc ccttcaataa
     3121 atgctcggct agcttctgca aaggactcat cctgataggt aagaggaatt tctaaatacg
     3181 accaatccca tctataaggg attagtgctg gatgctttcc gagacactcc tctgaagaga
     3241 gggagagaaa atcatgctca aaatttttct ttggaatcgg caaatgatat tcttcgatga
     3301 ttttcttgta ataaaacaac tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag
     3361 tcaaaaatcc cgactgatgc cattgctcca ggttttcttt tgttagtaac cgtggccatg
     3421 ccattgatcc gccttgcttt tggtttcttc ctcgagggta ataagggagc ccccttcttg
     3481 atccactgta tacaatcggt tccttgcctg atggatgata gacaagtttt cctttttcgt
     3541 tctcgaagcg cccttctctt gatactgtaa acaatccgac ataatcaaaa aaacttaagc
     3601 ctagacctct taaaaagaca ttttctttag gcggtatctt cgaaagatcg acatctgcag
     3661 gattagaagg aagctgatac agtaaatcat ttttctttgc gtagtctacc cacactcttt
     3721 cttcttctgt ctcatcattt tcccagtgac cgcttgctaa aacaagctga tcgatagaat
     3781 agctttgctg atctgtctgt aaaagataac catcttttct tttgctgact ttttttacta
     3841 aagaataaac aaactgaata gaagctggaa attcttcttg caactttgag aaaaaccagc
     3901 gttgatagag tccgtataaa catcgagtag attgctgatc tttttccaga cgattgattt
     3961 cttctataat tagttgctta tttttggtta ccgtctgttc gatataatct gtagcaggtc
     4021 ctttactcca ttgaaacaag ttgggtccct gtgttaccaa tcctccactg gacaatgttt
     4081 catctgtaaa aagcgtcact tgttgagaaa cagaattcat taataactcc ggtggctgat
     4141 tcaaccgcca aactcgacct ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat
     4201 gtctgtcctg attattatgg agaatccttt ctgctatact taatcctcta ggtccggcac
     4261 caatgatccc tattttcatt tgcatgcctc catttcgata aattttttca ataaaacagc
     4321 atggttctga tgttcatttt ttgcaccata aacaaaagta atgactggat gttctttcat
     4381 aagttgactg agtttaaaaa tagcttgctt cttttcttct tcgaaaatca attcttgctg
     4441 gtatttcttt gcaaatgaag caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc
     4501 attactaggt gcaatttctt tcaaccacag atctaactgc gctttttctt ttgaaattcc
     4561 tcgtggccag aacctatcca cgagtattct atagccatcc tctttttcag catcaaggta
     4621 aacacgtttt aatttgatca tagttgtttc cttctttctg ccttttcgct tagcttatat
     4681 atgtagataa tagcacaaaa taaaaaaaat gaacggcaca actatccatt gcgccgttca
     4741 tgtgatcttg gtcaaaatct aatgattgat tcggttttta aagctgtcat acaatcatct
     4801 gttttatact tttttacttt tgatttttcc agaccatccg tcatatccac ccttaagtaa
     4861 ataaatctct tgataacctg ctttacgtaa tttatttgct gtacggatgc tcaagctttt
     4921 cttttgatcg taaatataga caggctggtc ttttcgaata gatcctaatg aatctttcaa
     4981 aacagtgtaa gggatgtttc ttgcacctaa aatatgccca gcatcaaaca catctttttc
     5041 gcgaacatcg attacttgtg cttttctcat tccttctcga aattcttctt ctgtcaaagt
     5101 cgttgctgaa cgcttggcca ttactcgtag atagacttcc caaaagatga ttgcaagcac
     5161 aatcaatagc aaaatgatat taattaccca taaaaccatg taatgcctcc gtttttttaa
     5221 gcaaatttca aggctaatga ggcagcacca atgacaccag cttcattacc aagctctgct
     5281 aatttgattt ttgttgattc tcttacttgc gggaaagtaa attctttaaa atatttctct
     5341 acacggctac gtaaaaattc tcctgctgcc gatactcctc cacctaacac aatacttgac
     5401 ggattcaatg tattccccaa gttggcgcaa gctaaaccta agtagaagca tacgcgatca
     5461 acaaccatca atgcaaacgg atcgtcttct tgagccattt caaagatatc tttacttgtt
     5521 acttcttcac cgttgtctaa cattgctttc agttttgaat ctcctgcgta ttcttctgcc
     5581 aagtaacgac ctagacgtac cacaccagta gcgctagaca cagtttctaa acatccgcgt
     5641 ttaccgcaag tacattcgaa cccttcagga tccacagtga tatggccgat ctctccgcct
     5701 gctccgctca cgccgtgaat caaatttccg ccagcaacga taccaccgcc aacacctgtt
     5761 cctagagtaa cgaatacaac gtctggatcg ttttctccag cacctttcca tcgttcacct
     5821 aaagcagcaa cattcgcatc attatcgata gcgaatttga ttcctgttcc tgcctcgatc
     5881 agttttttca caaattgaac ttctgtccag tttaagttat acgcaccaat aaccgttcca
     5941 gcttcgctgt ccacgcttcc tggggtaccc atgccgattc cgataaagtc ttcggctttc
     6001 atattatata aattcaaacg atggttgatt gattcaatga tttcaggaac gatatgctta
     6061 ccatcatcca aaatattggt atcgatgctc catttttgtt gaatctctcc ttctggtgtt
     6121 aaaattgcaa attttgctgt tgttccacct aaatcaatcc caataatctt cttgtccatt
     6181 acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc tctttttaat atgcgccacg
     6241 ctgtcatgta tgtatcatga tcaatcaaac ggctatcata cagattacgc aattcgatag
     6301 tcattaattc aatgtcatag atgcgtgcac ccacatagat aaagatgcca aattttttga
     6361 acagctgctg tacatcatat aagctctcca tctttaagca cttccttcat attatacaag
     6421 taaaccatat ttttttaaac ccagtaaaat acatattaca aaaagaaata caaaaactag
     6481 tgtagaaaga atcctttggt gtatcgaata gcttgtctgt tgtttcggta gacccaaaac
     6541 attccctaac actaatccgc caattagccc accgacatgt ccccaaacat ctacagaggt
     6601 atccagaaga ccaaaaacaa atgtcaacac aataaataaa agaaaatgtc tgactagctg
     6661 ctggatcgct gggttatgtt taaaatgaaa ccctaagata aacaacgcac cgaacaaacc
     6721 gaatatcgaa gtactcgcac caccagacag aacattcgct tcgttaaaag caaagcttgc
     6781 tgtatttccc attatcccgc tgaacatata aatcaagaaa aaacgccaat ggccatagat
     6841 tgcctcgatt tgctgcccca taaaatacag tatcagtgag ttcattacga aatgcatcag
     6901 tccaaaatgg atgaagattg gtgtcactaa acgccaccat tcattgaagt gaacaagata
     6961 aggaccatac attccggcat aataagggat ctcaagtcgc ggaaataatt ccatcatgag
     7021 gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat aacggtcgtc ttaaccatgc
     7081 acgcaatttt atttgctgtt gataattcat gaaaacctcc catttcattt tagtcaataa
     7141 acaattgttg gatcttctga tcaaagctct ccggttgcca ttgttcattc aattgttcac
     7201 gaaaaacaag gctacatgtt tttttatgaa aattatttaa ataccggtcg taataaccac
     7261 cgccaaaacc gattcgataa ccggctctat tgtagataat acctggtaca atgaggagat
     7321 cgatcatctc cggcaaatag agcttctctg attgtggctc aagaacacca aaactcgaac
     7381 gtacaagaac ggactgttca tccatttcat aaaacgccat ctgacgtttc ggcaaagttt
     7441 taggtacaac tattttcttt tgttcttttt ctgctcgttg gataaccggc tgtgtatcca
     7501 gttcaaattc attcgataaa gtcgtcccta tcacttttgc tgacttccag tactcgctgg
     7561 cgaatagctg tttatagatt cgctgttctt tttccttttt cttgccactt ttggccagat
     7621 ttttcagctt ttcgattcct ttccggcgta atcttacttt caattggtcc aaaaactcac
     7681 ccttccttct ctcataaaat ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa
     7741 taaatgcata aatagaataa aaagaagacc tagaaatgat tggccttcct tttttaataa
     7801 atttcaactg ttcagcgcgt gtttttgtct gataaaaagt gcatacagaa aatatggcgc
     7861 accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca cttggtagaa tcactctagc
     7921 aaccatatct gcactaagca gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca
     7981 gttattttct ttacgtacga ctagcttcgc agcatgagga gctagtagtc ctacaaaaga
     8041 taaactacct gtcaccgcta cactaccgct tgccaaagca acagctaaca acaaaaggat
     8101 tttttgactt ttagccacgt taaggcctaa tccttgcgcg cgt
//
LOCUS       DANKHJ010000110         8128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000110 DANKHJ010000000
VERSION     DANKHJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="PW113_002764"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="PW113_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2527022.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="PW113_002765"
     CDS             complement(1378..1896)
                     /locus_tag="PW113_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527023.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="PW113_002766"
     CDS             complement(1912..2238)
                     /locus_tag="PW113_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2527024.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="PW113_002767"
     CDS             complement(2251..4230)
                     /locus_tag="PW113_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2527025.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="PW113_002768"
     CDS             complement(4251..4565)
                     /locus_tag="PW113_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2527026.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="PW113_002769"
     CDS             4733..5026
                     /locus_tag="PW113_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2527027.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="PW113_002770"
     CDS             complement(5104..6216)
                     /locus_tag="PW113_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2527028.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6603..6782
                     /locus_tag="PW113_002771"
     CDS             6603..6782
                     /locus_tag="PW113_002771"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527029.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6976..7146
                     /locus_tag="PW113_002772"
     CDS             6976..7146
                     /locus_tag="PW113_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527030.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7570..7953)
                     /locus_tag="PW113_002773"
                     /pseudo
     CDS             complement(7570..7953)
                     /locus_tag="PW113_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(7899..>8128)
                     /locus_tag="PW113_002774"
     CDS             complement(7899..>8128)
                     /locus_tag="PW113_002774"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527031.1"
                     /translation="YQLVETQAPIGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEILQELFLSYRTEKEKRYKPV"
BASE COUNT         2556 a         1642 c         1322 g         2608 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatccaat tcatacccta ttggcgcttg agtttctacc agctgatatg ctcctggtgc
     7861 taaaccgtca attgccagtt ttccatcttc tcctgtagtt agaccggttt gtagcgtttc
     7921 tccttctcgg ttctgtaact caaaaacagc tcctgcagga tttccccgct ttggtcatcg
     7981 atctttgtca acacgacacc gcctggtgta agtcggtttt ctttcgttaa ttctaccacc
     8041 gctgtctgac ttctttcgat ctcaaactcg attggtgtcg catctaattc ataccctatt
     8101 ggcgcttgag tttctaccag ctgatatg
//
LOCUS       DANKHJ010000111         8095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000111 DANKHJ010000000
VERSION     DANKHJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            4..1458
                     /locus_tag="PW113_002775"
     CDS             4..1458
                     /locus_tag="PW113_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL2527032.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="PW113_002776"
     CDS             1534..2694
                     /locus_tag="PW113_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDL2527033.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="PW113_002777"
     CDS             2807..3814
                     /locus_tag="PW113_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2527034.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4863
                     /gene="iolG"
                     /locus_tag="PW113_002778"
     CDS             3838..4863
                     /gene="iolG"
                     /locus_tag="PW113_002778"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL2527035.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            4880..5770
                     /gene="iolE"
                     /locus_tag="PW113_002779"
     CDS             4880..5770
                     /gene="iolE"
                     /locus_tag="PW113_002779"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL2527036.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5793..7388
                     /locus_tag="PW113_002780"
     CDS             5793..7388
                     /locus_tag="PW113_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL2527037.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7404..7718
                     /locus_tag="PW113_002781"
     CDS             7404..7718
                     /locus_tag="PW113_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2527038.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
BASE COUNT         2409 a         1534 c         1939 g         2213 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgag tcgtatcagt atgcgaagaa aatggtagaa
     4261 gatggcgaaa tcggcgaaat cacactgatt cgttgttatg ggatcgatcc gagcgaaggc
     4321 ttggaaagct ttgtcaaatt tgccatgaat aacgacagcg gcggcttgtt cttagatatg
     4381 gcgatccatg atattgattt ggttcgctgg tttaccggcc aagaagtcga tcaagtgtgg
     4441 gcgattggga aaaatgcggc gtatccagaa ttggatcagg ctggtgagct tgaaactgga
     4501 gccgcaatga tgagcctgag cgataaaaca atagctttac tagttgccgg cagaaacgca
     4561 gcccatggct atcatgtcga aacagagatc atgggaacca aaggaatgct gcgggtagcg
     4621 caagcgcctg agaaaaatct tgtgacgatc atgaacgatc aagggatcat ccgtccatgt
     4681 tcacaaaact tcccagaacg attcagagaa gccttcattc gtgaagccca agaatttgtc
     4741 tcatgtattc aagaaaaacg tcaaccagcg gttaatgcag aagatggatt gcaatcaact
     4801 aagatcgcgt tggcttgtca agaatcattc gatacgaaga gtttagtaca actaaaaaaa
     4861 taagcaggag tgtgagaata tgaccaaaca aattcaatta gccatcgcac caatcgcgtg
     4921 gaccaatgat gatatgcccg agcttggtag tgaaaatacc tttgaacaat gtatcagtga
     4981 aatggccctt gctgggttca ccggaacaga gattggcaac aaatatccaa aagatccaga
     5041 ggtgttggaa caatatttga atattcgcgg cttaaaggtt gccagtgcat ggttcagtgc
     5101 gtttctgaca actaaacctt ttgaagaaac tgccgaagcc ttcaaagcac atcgggattt
     5161 cttacatgcg atgggcgcta aagtcatcgt agtagctgag cagggtcata gtatccaagg
     5221 gattatgaca gcgccagtct ttgatgataa accagttttc actgatgaag agtggaaaaa
     5281 actggcggaa ggattagaaa aattagggga tttggcccac gaaaaggata tggagatcgt
     5341 ttaccatcat cacatgggta ccggcgttca aacgacggaa gaaatcgatc gtttaatgga
     5401 aatgactgat ccagacaaag ttaaattatt gttcgatacg ggacatttgg tcttttctgg
     5461 tgaaaatcca atcgcggttt acaaccgtca tcaagatcgt atcaaacaca tccatttcaa
     5521 agatattcgt caagaaacgg ctgaagaagt agaaaaggaa aaagacagct tcttaaacgg
     5581 cgtgaagaaa ggcgtcttca cggttcctgg tgatgggatg attgatttca ctccaatctg
     5641 gacagcaatc catgccagcg actatgaagg ctggatcgta gttgaagcag aacaggaccc
     5701 ggctaaagcc aatccatttg aatatgcagt caaagccaga agctacattc gagaggtcac
     5761 cggtctttaa tctattgaaa aggagctaga cgatgaatct tttttctata atcagtttcc
     5821 tagtgattgt catctgtgta tggttatttg cctattctag atccagaagt gtcgatactt
     5881 ctggatcaga agggttcttc atgggagggc ggagcttaac tgctttgcca atcgccggca
     5941 cgatcatcat gacgaacctg tccaccgaac aaatagtcgg acaaaatggt caaagttatc
     6001 atgcagggat ggaagtaatg gcctgggaag taactgctac gattgcgatc gtagctttgg
     6061 ctgtgatatt cttgccaaaa tattttaaat acggcatcaa tacaatctca gatttcatcg
     6121 agattcgtta tgatacggtt accaaacgaa ttatttcgat cttatttatt gtgacctata
     6181 tgacttcttt cttacccgtg gttctttatt ctggatcgct ggtgttcaat aagatcttcc
     6241 atattgatga gctgctgggc gtccagccga tcgtcgcgat cataattgta gcgatggcaa
     6301 ttggtattgt cggcatcctc tatctgctga tcggcggctt gtcgttaagt gccaatagtg
     6361 actccattta tggagtcggt ctgctggctt gtggtctgct ggtcccaatt ttaggcttaa
     6421 tgaagcttgg tgacggcagc tttgttggcg ggatcgaaca cgttgtggta aacacgccat
     6481 ggctgcttaa ctctgtggga tcgatcgact cggctgttgt tccatggccg acactattta
     6541 cagggatgct gtttaacaat ctgtatttct ggtgtaccaa tcaaatgatc gtccaaaaag
     6601 cattgtccgg caagaattta gctgaggcac aaaagggcgc tttcctagtc ggcgtcttca
     6661 aaatcttagg tgcgttattc cttgtctttc cagggatcgt cgctcgcaat atttttggcg
     6721 atagtttaat gtcaaatcca gataacgcgt atcctttttt agtcagtgaa gtattgccta
     6781 aaatgatctt tggtattttc gccgctgtta tctttggcgc gatcttatca tcatttgcgg
     6841 gagctttgaa cgcgacatcg accttgtttt cactggattt ttataaaccg cttatcaata
     6901 agaaagcaaa tgataagcaa atcgctcgtg ctggtaaaac agtaacggtc gttgtgggga
     6961 ttatctccat tatcgtagcg ccattcattt cttttgcacc ggctggattg tatcagttcg
     7021 ttcaagaatt caatggacta tacaacatgc cgctgctcgt gatcattctc tttgcctttt
     7081 attcaaaaaa agcaactgct tttggggcaa aaacgacgat tgccgtgcat atcgtcttgt
     7141 atgcactatc aaaaatgctt ttccaaaata tccatttcct ttatgttttg acggcattat
     7201 tcgttctgga cattatcatt ttgatggtgg tttccaaatt gaaacctgat ggagaatttg
     7261 tgttgaactc attcgataca aaggtcgata tcgagccttg gaaatacacc aaggtgactg
     7321 ctatagcatt ggtagtcatt gttattttaa cttatgtggc attttcacca attggctttg
     7381 caggctaaaa acaggagggt attatgcata aggatctatt gattatttgc gagaccggaa
     7441 tcagtgcgtc gctgctcgta tcaaaaatgc ttgaaacgat cagagccaaa aatctatcgt
     7501 atgagataga ttatgcaacg atccgaaacg ttgaagaaaa actgaattat aaagactatg
     7561 acgttttact tttaacgccg caggtcgcac gttctgagga aaaagtaaaa gaaatcatca
     7621 aaaaggaaaa atgtaccgct cagatcgttt atatcaaaca agaagatttt cgttatatga
     7681 atattgaaaa catcattgca agcatcgaag aagattaacg agaatctttg attattgagt
     7741 gtcgaggagg tggtatatga aaagatggca tgttcgcccc gatcacccca atatgaaggg
     7801 cggacttatt agaaataccc aatcttgtat ttattcaata gggtaagcgg acctcaatag
     7861 ccagctcatc cccaaaaagt cgaatggaga cacttaaatg agtgtaccta ttcgactttt
     7921 tgagactcta agtcaaatcg tgttgttctc attaaatcac tgaaaggaaa tgcttcggcg
     7981 tttccttttt tctttttgtt tttcttcgtg ttttgaaaat tacttactat ttactatacg
     8041 agtagtttca aataattttg acatcctaat cttcaacaac agaaaaaaat ctaaa
//
LOCUS       DANKHJ010000112         8035 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000112 DANKHJ010000000
VERSION     DANKHJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..199)
                     /locus_tag="PW113_002782"
     CDS             complement(<1..199)
                     /locus_tag="PW113_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527039.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="PW113_002783"
     CDS             complement(362..1996)
                     /locus_tag="PW113_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2527040.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="PW113_002784"
     CDS             complement(2000..3373)
                     /locus_tag="PW113_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2527041.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="PW113_002785"
     CDS             complement(3389..3628)
                     /locus_tag="PW113_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527042.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="PW113_002786"
     CDS             complement(3810..6485)
                     /locus_tag="PW113_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL2527043.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="PW113_002787"
     CDS             complement(6668..7591)
                     /locus_tag="PW113_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527044.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2361 a         1580 c         1207 g         2887 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatattgatg ttttttctct cattgtttac aaactagata gcttatgtat tcttattaaa
     7981 aataatagat atttcgaaca tcatcactct catctactta atttcgcatt tttac
//
LOCUS       DANKHJ010000113         7994 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000113 DANKHJ010000000
VERSION     DANKHJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..323
                     /locus_tag="PW113_002788"
     CDS             <1..323
                     /locus_tag="PW113_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2527045.1"
                     /translation="VAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPFLIIGGFLWV
                     FSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILIALSAIFMLF
                     SLLG"
     gene            complement(393..2054)
                     /locus_tag="PW113_002789"
     CDS             complement(393..2054)
                     /locus_tag="PW113_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2527046.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2600..3148)
                     /gene="aac(6')"
                     /locus_tag="PW113_002790"
     CDS             complement(2600..3148)
                     /gene="aac(6')"
                     /locus_tag="PW113_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2527047.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            3700..4803
                     /locus_tag="PW113_002791"
     CDS             3700..4803
                     /locus_tag="PW113_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2527048.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(4996..6063)
                     /gene="rlmN"
                     /locus_tag="PW113_002792"
     CDS             complement(4996..6063)
                     /gene="rlmN"
                     /locus_tag="PW113_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2527049.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(6293..>7994)
                     /locus_tag="PW113_002793"
     CDS             complement(6293..>7994)
                     /locus_tag="PW113_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2527050.1"
                     /translation="KKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPANL
                     EKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVDQ
                     SDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDD
                     IALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRD
                     VSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVEL
                     NMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNL
                     ENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVD
                     VITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGA
                     FISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQT
                     ASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM
                     "
BASE COUNT         2207 a         1687 c         1391 g         2709 t
ORIGIN      
        1 cagtagcttt tctactgatt ctggttattt taataaaaaa tctcatcaac cattccttta
       61 cactcattca attatctaat gatctattct tatggtcatt gccgtttctg attatcggcg
      121 gttttttatg ggtcttttct tcgggtttct tcgatcattt tcagcgatcg gtccatcttg
      181 cacgaacaag gaaccgaaag aaaaaaccag aattttccac gctctcgtct gccagctacg
      241 gtatgtacag cttttggctg atcattgccg gaatactaat tgctctttct gctattttca
      301 tgcttttttc tttgttagga taagtgaata tgagaacgag gttgcgacat ttgccgcagc
      361 ctcgtttttt ctcatgctca cggtattaac gattattctt caacgtatgt ccatttgtaa
      421 tcccatgaag cgcctgccgc atggtgaacg atacctttga ttttttcatt cactaggtga
      481 ccttctgctt tttggtacac cggaacgaca cccatatcat cagagatgat cttgattgct
      541 ttttggaaat catcccaacg ggcttgttca tcgccagcgt ctgttgatga agctgcttta
      601 actgcttcgt catattctgc attgctccag cgaccacggt tgtatgaatt acctgtgaca
      661 aacaaatcag taaagctgct tgggtctgca tagtcagcag cccatccgcc tagtactgcg
      721 tcaaaatcac ccgaagttga gcggtcaaga cgtacagaga atggaacagg tgttggtttg
      781 actgttacgc catccaagtt ttcttggatt gagttttgaa catattcgat tacttttttc
      841 gatgaatcat catcagaagc catcaaatca aattctaaag aatcaacgcc caattctttt
      901 ttcgcttttt cccaatattc ttttgcttta tctttgtcat aagaaactaa ttttccagct
      961 tcttttgcaa agtcttcgtt cgttgttgga ctttttacca tatctgatgg gatcaatcct
     1021 gtagagacaa ctgatccgtc acctagtact tgttttacta aagcttcacg atcgatagaa
     1081 taagaaatag cttttcttag gttcacattg ttgaatggtg aattcttttc acgttgattg
     1141 aattcgatat agcttgtccg cgcttcttta acagaagtat aagctggatc gtttgcgttt
     1201 tgctgtgcta actcaccaga caagatcacg tcatctgctt ggccatcttt gaataagttc
     1261 aatgcagtag aagattcttt tactacactg actttgattt cagaaagttt tactgtatct
     1321 ttgtcccagt aatgatcatt tttcttataa gtccattctg tatctgttcc tggtccatca
     1381 aaatcttcta atgtgaatgg tccattataa acagctttat cacttgcaga tgcataggca
     1441 tccccatttt cttccactac ggattgtttt tgtgggaaaa atgatgggaa agcaagcaga
     1501 tattggaaat atggtgtttg tttcgtcaat ttgatttcta attcatagtc accaactgct
     1561 ttgatcccta gttctgattt atctttcttt ccagctgtaa tgtcttctgc attttcaact
     1621 ggcgcataca ggtaagcata ttcagaagct gtagctgggt ctactgttcg ttgccagcca
     1681 taaacgtaat ccgctgctgt aacaggatca ccatttgacc attttgcgtc ttctcgtaat
     1741 ttgattttgt aagttaatcc gtcttcactg acttctgcca actcgctagc tcctgccggc
     1801 tgtggtttgc tgtcttcatc aagacggtaa attccttcat aaacgttatt caatgctgta
     1861 aaactgattg tatctgttgc aactgataag tcagctgtag gcatttcttg aggcacgact
     1921 aagttaaata cgcctcctcc atctgctgaa ccgctttttg ctgaactact gcttgtggct
     1981 gaactgccgc cataacatcc agcaagtaca aggccgcata ccgcaaccaa accaaatgta
     2041 aatttctttt tcattttacg taccccttta ttggaatttt cagaataatt aatattttct
     2101 gaagttgttt aagagtttaa tgaataatta ttaaaaaatc aacacttttt tttgtctttt
     2161 tcataaaata atgatttttt cgtgaggttt caaggaattt ttcagaaaaa atagctttgt
     2221 acaaggtttt ccatggtctt ccaaaagcca ttaaataatt ttcgttatat tgtctattat
     2281 tgccatacaa agataaaaaa gaataaacaa aaagaaactg tgacaaaagt cttgtcacag
     2341 tcccttatct gaataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
     2401 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
     2461 ttcacaacct ctggtacacg gtgttccaaa agtagcttct tcggaaataa gttggaattt
     2521 cacaaaaatt caagaagtaa ttttcgtaaa ttccttctta tttcttgaag ctgattactt
     2581 ctgtcacaac ctctttttat tattgagaat ttggtcgagg aataatcgtt tttgccatcc
     2641 aaatatccgg tttgtcccag ccatttgcat ttggtaagac acctacgatt ttataaccta
     2701 atttttcata gaattcatac ggatgttcac gaaggttctg gatggaagcc actttatcaa
     2761 atgtatgctc atacaggtcc gtttgactta acgttgttcc atggtctaaa tcatccgtac
     2821 ctaaataaat cgtgattcct cctctggaag ctacttcttt ttctaagtaa ttgactaatc
     2881 gagtacctat ttggttcttt cgtcgggagc tttctacaac taatggatgc aattcccaac
     2941 ctgtgatacc gtattgaggg attgcaccaa taaatcctac taactcatct tggtctaccg
     3001 ctgctaccgc gattcgttct ggattcatca tttcttctac ttcttctgcc gagctgtctc
     3061 catattcttc cggccaagtc agtctcagta aatcagaaag ctgatctttc aatactggat
     3121 tattacggtc aaattcactg attatcatac gtcttccccc ttgttcttgc ttattctaac
     3181 agtccttttg aaaaaacgaa atgttctcgc tcaaaaatct ttagctacca atcagtatct
     3241 ctattctttc attatttatc ttacttataa aataaaaaat tatacagaaa cactttattc
     3301 aaaataggaa agatcaggaa agccatcaat tggaggaaaa aactctgttt gttcatcatg
     3361 atatagatta tcttttgctt taaaaaaaac caattatgaa ttgacaaata aaacctgttt
     3421 tcatataata atcttaattt ttagaatttt tagataatta caaaaaagca aagggaatcg
     3481 gtagaaatac taagaggaac aaaaattcat tattgtcgtt ttcaatgttt ttgtacttgt
     3541 tcgtttttag gatacttatt gatgatttca aggtgttcat gccaccttat tcatcactta
     3601 tcctgtctta ggttcacctc tactccatct acagccgcag tcagatcgat cactctttaa
     3661 aattttaacc cgtttcaacg aagaaagaag gaaatgaata tgagtcaaag tgttttctta
     3721 ccaaattata ccattggaac agatgcgtat gaaaaagcaa atggaatcgt caaagaatat
     3781 gggacaaaag tagtgctgat tggagggaag actgcacttt caaaggcaga aaacaaaata
     3841 aaagtagctt tgacacagga aatagaggtt gtcgccagtc tctggtacgg gggtaattca
     3901 acttatggaa atattgaaca gttgatccag catgaaagtg tccaaaaagc agacgtcctg
     3961 tttgcagtcg gtggtggccg tgcttgcgat actgtaaaag tcattggtga aaaactagct
     4021 aaaccagtct tcacctttcc cactattggt tcgaactgtt cgcccgtcac tgcggtatgt
     4081 gtcatctata acgaagaagg cgtaatggac ggactcttct ttccttcaaa accacctgta
     4141 cacaccttca ttgatacagc aattattgcc gaagcacctg ataaatactt atgggcaggt
     4201 attggcgatg ctttatccaa agaagtcgaa tctacttttt ctgctcgtgg cgatgatcta
     4261 ggctacacgg atagattagg aatccaaatc ggaaaaatgt gcattgatcg tctcttgact
     4321 tatggtacgc aagcattgaa agatagtgcc gacgatcgtc cctcatatgc aattgaacaa
     4381 gtcgtttcgg atatcattgt tactaccgga ttggtttctg ttttagttaa caataactat
     4441 aattctgcta tggcacactc attctattac ggtacgactg ttctagaaaa aatggaggga
     4501 taccttcacg gagcatctgt cagttatggt gtactgacct tattgatgat ggatcagcag
     4561 ttcgatttgt ttgataaaat ctatgtgttt atgaaaaaga accaacttcc agtatcgtta
     4621 accgacatga atctcaatgt cacgaccgat ttagaagcaa tcttagataa agccatggca
     4681 acaaatgata ttaaacatgt gccttatgaa atcacccgag aaaaatttaa acaggccatt
     4741 cttgacgtag aaacttatca tgctacacaa gaaagccagc aaggatataa gaaaatcagt
     4801 taaataattg ctaaaacccc catcaagcag gctgtcatca aattgaggac atttctttgc
     4861 ttgatgaggg tttttcttgt caaaaaaagc tttatagctt tcgctataga atcttttctc
     4921 ttatttaagt tgaacagttc cttaattaag aaaactgctc cttttatggt aattgaaaaa
     4981 acgagtagga tacgattatt tggcaactgt ttttttcatt tgcttgcttc gcaattgtcc
     5041 gcaagctgca tcaatatctg taccatgttc tttacgtatc acacagttta cgccgttttt
     5101 cttcaataca tcataaaatc tcaatacatc agcttttgga ctgcgagaat attgatcatg
     5161 ttcactgact ggattatatg gaatcaagtt gacatatgct aattttttct tatcttttaa
     5221 tagatctgct aattgttgcg catgctctgg tctatcattt acttgattca acatgatata
     5281 ttcaaacgtg attcgacggt ttgttttctc taaatactca tccacagctt ccatcaattt
     5341 ctcaattggg aaacttcggt tgatccgcat gatcgatgtt cgcacctcat tattcggcgc
     5401 gtgcaaggaa atagccagat tgacttgcaa gccgttatta gcgaattcct tgattttgtg
     5461 tgctaatcca ctagtggaaa ctgtaatgtg ccgagcacca attgccaatc ctttggcatc
     5521 attgatgata tgcaaaaagt ccattacatt gtcgtaatta tcaaatggct caccgatccc
     5581 cattacaacg acatgtgaaa cgcgttcgcc aagattgcgc tcatcaaaat aatgttggac
     5641 catcatgatc tgtgcaacaa tttctccagc agtcagatca cggttctttt tgagcagccc
     5701 gcttgcacaa aacgtacagc cgatattaca tcctacttgt gtcgtcacac aaactgacaa
     5761 gccatattct tgtctcatca gtacagtctc aatcatgttt ttatcaggga gttcaaacaa
     5821 atattttact gtaccatccg aagcttcttg gacgaccatc tgttttagag gattgatgat
     5881 aaaatgctcc gataattttt cgatcaattg tttcgataga ttcgtcattt ctgtgaaatt
     5941 acttacacgt ttttgataca accattccca aacttgggca gctcggaatt ttttctcgcc
     6001 gttttctata aaccaattga ttaattcttc attcgtcaag ccataaatgg actgtttttc
     6061 caaagtattt tccctctttt tctcaaagtt tcgctacata ctatacatga ttcatttcct
     6121 taatgcaata cttcacaaat aaatcgaata gaactttaat ctcacaaaaa ctaaaaagac
     6181 aagacatcat accgtattag aaaagatgga aaaaaaccat tttttctttt ggtatcatca
     6241 tgccttgtct ttttattggt tattcgtttt tcaacccttc atcacacaag ttttacatgt
     6301 cattatcgtc cggcaacgtt tctgatgttt cttcctgctc aatgaatact ttccgaggct
     6361 tgcttccttc tgacgggcca acaactccat gagcttccag ttcatctact aaacgtgctg
     6421 ctcggttgta accgatccga aatcttcttt ggagaaggga aatacttgcc gtttgcattt
     6481 caacgacaag tgcttttgct tcttcaaaca gctcatcttg tggctgttct ggagcacctg
     6541 tcgtttcaac ttcatctgtc ggcatcattt tctcttcata atgagcttct tgctgatctg
     6601 taacaaattg aacgacccgt tctacttcgt ggtcagaaat gaatgctcct tgcacacgga
     6661 tcggtttatt ctcacccatt ggcaagaata gcatatctcc tcgccctaag agcttttctg
     6721 ccccattaga atcaatgatc gtccgagaat ctgtaccgct tgataccgca aatgccattc
     6781 tggaaggtac atttgctttg atgattcctg tgataacatc cacacttgga cgttgagtag
     6841 ctaggatcat atgaatcccc gctgcccgag ccatttgcgc taaacgaata atcgcatctt
     6901 caacttcatt gctggctacc atcatcaaat cagctaattc atctacgatc actacgatga
     6961 atggtaaggt tggatgtttc gttccgtttt ccaagttttt ctgttgaaca aattcattgt
     7021 aaccagaaat atttcttact ccggttgccg cgaatttttc atatcgttct tccatttctt
     7081 gtactacttt ttgtaaggct tgcgctgctt ttctagggtt cgtcacgacc ggcgtcaaca
     7141 gatgaggtat gccgttgtac atattcaatt caaccatttt cggatcgatc atcatcagtt
     7201 ttacttcatg gggcttagca cgcattaata tacttgtgat aatcccatta attgctactg
     7261 acttcccact accagtcgaa ccagccacga gtaagtgagg catcttcgaa agatcagccg
     7321 tttgtacccg tccagaaaca tcacgtccta aaggtacttc tagtaattta tctgggtggt
     7381 ttggttgtgc ttcgatgatt tctctaaagg aaaccatact gatctttcca tttggtactt
     7441 caataccaat cagtgattta cctggaatcg gtgcttccat ccggacatct ttcgccgcta
     7501 aagcaagcgc gatatcatct gttaagttca caattttact tacttttacc cctacagccg
     7561 gctggacttc gaattttgtg acggatggtc ctaagctggc tttgacgacc ttggcatcga
     7621 caccgaaact ttggaaagtt tgttctaata cgccgatatt tttttcaatt tttttatact
     7681 catcactttg atccacagtt ggtatagaat caagcaaatc aactgtcggt aattcatagt
     7741 cttgattttc ctgttctgct tcaatctcaa attctaatga ggtaccgtca tcctctggca
     7801 actcatctga aaaaccttct gctgtattgg tctgttttgg tgtatctggt actggtttct
     7861 ctagattcgc aggatggata ttttcttgaa aactgtcgat tggaacaaaa cttagctgct
     7921 ccggctctgt attttctggt tcctgccatt catcagcagt attttccctt tgctggctca
     7981 ccgctttttt cttc
//
LOCUS       DANKHJ010000114         7939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000114 DANKHJ010000000
VERSION     DANKHJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            274..1002
                     /locus_tag="PW113_002794"
     CDS             274..1002
                     /locus_tag="PW113_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2527051.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            1020..2216
                     /locus_tag="PW113_002795"
     CDS             1020..2216
                     /locus_tag="PW113_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2527052.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            2209..3204
                     /gene="lacD"
                     /locus_tag="PW113_002796"
     CDS             2209..3204
                     /gene="lacD"
                     /locus_tag="PW113_002796"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2527053.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            3201..4142
                     /locus_tag="PW113_002797"
     CDS             3201..4142
                     /locus_tag="PW113_002797"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2527054.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            4318..4797
                     /locus_tag="PW113_002798"
                     /pseudo
     CDS             4318..4797
                     /locus_tag="PW113_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4810..5700
                     /locus_tag="PW113_002799"
     CDS             4810..5700
                     /locus_tag="PW113_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2527055.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            5687..6496
                     /locus_tag="PW113_002800"
     CDS             5687..6496
                     /locus_tag="PW113_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2527056.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            6510..6911
                     /locus_tag="PW113_002801"
     CDS             6510..6911
                     /locus_tag="PW113_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2527057.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(6957..7907)
                     /gene="yidC"
                     /locus_tag="PW113_002802"
     CDS             complement(6957..7907)
                     /gene="yidC"
                     /locus_tag="PW113_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2527058.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         2535 a         1308 c         1729 g         2367 t
ORIGIN      
        1 aataaaacga tagttgtagg aatatcatag ggattaatgt ggatcacgct gctcgataag
       61 ttagaccatt ttgacggatc gcttatgtgg ttcgtctttt tttctgttct tttattggta
      121 actggtattt ttgaggtatc tttgtctatt caagatataa agctttaaag agctgttata
      181 atagcttttt tggtctaaaa aacctttgac aaatgattaa taattcacta ttcttgtgtt
      241 gtggttataa ccagtaaaga agaggtgatt tttatggaga gctcaaataa aggaaaatca
      301 ttgtattatc aactagttga tttattgaag gatcgtatcg aaacacagat gactccgcat
      361 gataagctgt tatctgaaag agaactttgt caacactatg gggtgagtcg taccaccgta
      421 cgtctggctt tacaggaatt ggaaaatcaa ggttatattt accgaagaca tggaaaaggt
      481 acctatgtct ctgatttgtc tgataagtca tcgaacttag ctggagcata tagctttaca
      541 gaagaaatgc gcgaaatagg gcgcgtccct aagacggtca ttttagaatt tagacaaatg
      601 gaagctaata agagaattgc taagaatttg aacatccatt taggtgaaac tgtatttaaa
      661 cttaaaagat tgcgtttggc agatgaagag ccattgatgg ttgagcgttc ttatctaccg
      721 gcgaaactat ttatgggact tgatttgccg ttacttgaaa gtaaaccgtt gtatgatgtg
      781 ttttcacagg attatgaaca acaagttcaa ttagctgatg aagagtttta tgcaagtatc
      841 gtgcaaaaaa aagatgctgt tgtgctacga atcgctgatg gcgctcccgt cttacatctg
      901 acgaggataa cctataacgt gaaaaatgag attatcgaat atacagatag tgtagcaaga
      961 gcggaccagt ttcattataa aattcggcat atccgaaaat gattgagagg gagaacaaca
     1021 tgtttgagct atcgaagaac aaattaaaac aaataggtgc agcaatcacg acacaagaaa
     1081 taaaacaaca accagatctc tggaaagaaa caatcgatag attgatgcaa agccaagaaa
     1141 atttagctct attttttgat caaataaaaa aagaagcaaa cggaagaaga atacgtgtta
     1201 tttttacggg tgcaggaacg tctcaatata ttggggatac gctcttaccc tatctaaatg
     1261 aacatggaga caatcagttt tttatcttcc acagttttgg cacgacagat attgtagctt
     1321 caccaaaaga atttttgttt ccaaatgaac cgactttatt agtttcattt gctcgcagtg
     1381 gcaacagtcc agaaagtgta gcggctgtgc aaattgcaaa tgaacaagtt gaaacattgt
     1441 ttcatttgac gattacttgt gctaaagagg ggatgttggc aaaaaacgca caggatgata
     1501 aacagaatta tctgttattg atgccagaga gagcaaacga tgcaggtttt gctatgacag
     1561 gaagttttac gtgtatgtta ttgagtgctt tatggatctt tgatcaaaca gcaatggaga
     1621 aaaaagaaag atatcttgaa caattagtta aaatcggtca agatcttctt tcaagagatc
     1681 aagaaattca aaagttatta cctgattttt ttgaacggat cgcttatctg ggttcaggaa
     1741 gtttggcagg tgcgacaaga gaagcacaat taaaaatttt agaattaaca gcaggtaaag
     1801 ttgctacaat ttttgattct tcgatggggt ttcgtcatgg accaaagtct tttttaaata
     1861 gcgagacact cgttgtaggt tttgtgagca atcaaccata tacgcgaaat tatgacttag
     1921 atattttaga ggaaataaaa gaagatcggg tcactgccaa tgtcattgcg attgcgcaac
     1981 cgggtactat tgatttctca ggtatgactg tgctattgga agactcggtg agtaaattgc
     2041 ccgatgccta tcttgcatta gcgatgatta tgactgcaca aatcattgca ctctatactt
     2101 caatcaaagt agctaataaa ccagatacac catctccaac aggcacggtc aatcgagtag
     2161 tcaagggcgt cattattcat aagtatgaaa ccgaaggaga aatgacagat gaatgaagaa
     2221 aaaaataggg caatggagag actatcaaca aaagatggga ttattagtgc attagcgatc
     2281 gatcagcgtg gtgctctaaa aaaaatgatc aaatcgttgg gaaaagagcc aagtgaagac
     2341 caaatcagtg aattcaaaga actagtatca gctgagctga caccctatgc ttctgctatt
     2401 ttattggatc cggaatatgg attgccagca gcaaaagtca gagcaaaaga gacggggtta
     2461 ttgttagctt atgagaaaac cggctatgac gcaactacgc ctggtcgtct acctgattta
     2521 ttagcggatt ggtcttctct cagattgaaa gaacagcaag cggatgcagt aaaattctta
     2581 ctttactacg atgttgatga agcacccgaa atcaatcatt tgaagcatgt gttcatcgaa
     2641 cgtttaggta gtgagtgcga aggagaaaat cgtcccttct atttggagtt agtttcttat
     2701 gacgcacaaa taagcgatag ttcttctcca gagtatgcga aagtcaagcc ccacaaagtt
     2761 attgagatga tgaaagaatt ttctgatcca aaatataaag ttgatgtgtt gaaagtagaa
     2821 gtaccagtca atatgaatta tgtgaatggc tattccacag gtgaatttat tcacagcaaa
     2881 gaagaggctg ctgccttctt taaggagcaa agtgaagcga ctccattacc attcattttt
     2941 ttgagtgggg gtgtttcaac agaattattt caacaaacac tgatttttgc tcatgaatcg
     3001 ggttccacct tcaatggtgt attgtgtgga cgtgcgacat ggaaagatgg ggtcaaagca
     3061 tacgtcgaaa atggaaaaga cggagctatt cattggttaa gaacaaatgg gagagaaaac
     3121 atcgagactt taaataaagt aataaatgaa accgctaccc cttggttcaa taaaacaaaa
     3181 agcaaaaagg tgaagatgaa atgattacga cagttacctt gaatccttcc atcgatatcg
     3241 cgtatacaat tgaagaatta aacctcgaca aagtcaatcg agttgaaaca gtcaataaaa
     3301 cagcgggagg aaaaggagtg aatgtggcga gggttctaaa tttattagga gaagaggtga
     3361 tagctactgg tttaataggt ggccagcacg gtgaatatct aaagaaccag ctagatcagt
     3421 tagggatcat gcatcagttt gttccaatta aaggagagac aagggattct attgctatcc
     3481 tgcatagttc gagacaaaca gagcttttgg aacgaggccc cttcataaca aagaaagaac
     3541 aacagacatt taccgatcat ttcgagcagc tcctcgaaat atcagatatt atgacgatct
     3601 caggtagttt accccaagga tgccctcttg attactataa gcaattggtt gctttagctg
     3661 ataaaaaagg gaaagacata ttactagata cttctggtaa atttttgctt gaagtattgg
     3721 aaggtaagga gaagccacta cttatcaagc ccaatctgga agaattagga gaactgattg
     3781 gccaacgtat ctgttcttcg attcaattag aaaaaattca gtccttatta gctcatccaa
     3841 tatttgaagg agtgccatgg gttgttgttt ctttaggaga gagaggtgct gtggcaaaag
     3901 ttgctcagtg tttttacgaa gtcattattc ctaaaatatc agttgttaat cctgttggtt
     3961 caggtgacgc gacccttgca ggatcggccc atgcgttatc aaaaaggcaa tcaatcgaag
     4021 agattctcgc attttctatg gcttgtggta tggcaaacgc gcaacaagag aaaactggct
     4081 ggatcgaccc tatgaaagtt acgatgttga tgaaacacat tcaagttaag aaaatcaaat
     4141 gaacttaaat ataaaagaaa gcgttttctt tagttgggaa ggaatttgtt cttagcttaa
     4201 aaatgaaata taagaaagaa aaaatgaaaa cgttatcttt gaaacagaag ggatatttga
     4261 tgatgtcaca gttttaattg aacaataaat agaaaaaata gataaggaga atgcatgatg
     4321 gcaattattg gtgtaagaat cgatggacgt ttgatacatg gataagtagc aaatctttgg
     4381 acaacgaaac tgaatattag tcgaatcatg gtcgtggatg atgaggtagc cacaaacaca
     4441 attgaaaaaa gtggattgaa gttagcgact ccagcagggg taaaattaag cattttgcca
     4501 atcgaaaaag cagcaacaaa tattttagct ggcaaatatg actctcaacg agtattgatc
     4561 gtagcaaaaa agccagatcg aatcttatct ttagtagaaa aaggagttcc tatcaaagaa
     4621 gtaaatgtgg ggaatatgtc acaaacgaat gaaacacggt ccattacaaa atcaatcaat
     4681 gtggtagatg cagatatcga agtatttaaa aaattagatc agcttggtat acatttaatt
     4741 gctcaaatgg taccaaacga taaagcagaa gactttatga atcttttaat caaataggag
     4801 gaatgatcca tggatattca atggtggcag atcttattat taacattata tgcaggttat
     4861 caaattttag atgaattaca gatctattct tcaatgagtg cgccagtgtt tgcagggtta
     4921 tatgcaggtt tagtaatggg tgatttaaac gcaggtttga ttattggtgg cagtatgcag
     4981 ttgacagttt taggagtggg gacgtttgga ggcgcatcaa aaatagatgc caactctggg
     5041 acgattttag caactgcctt ctcgatttct cttggaatga atccagaaca agcgattgct
     5101 gctattgcag tgcctgtagc aagcttaatg atccaattgg atattttagc tagattcgct
     5161 aacacatact ttgcccatcg tattgaccgt cacgtagaag acatgaatta tcaagcaatc
     5221 gaacgcaatt tcttaatggg agctttacct tggtcgctct ctcgtatgat tcctgtattt
     5281 ttagctttag catttggtgg tggattagtt gaaaatgttg tttctgtttt aaacgggaat
     5341 ctaaaatggc taggcgatgg actttcagta gcaggagctg ttttaccagc tgttgggttt
     5401 gccattcttc tgcgctattt accagtaaaa aaacatctat cttatcttat tttaggattt
     5461 acgatcacgg cattattagc aacgttattc tctaatgttc aattattagg aacaagtgtg
     5521 gcaagcgttg ttaaagatct tccggcaact ttcaattcgc tgccgatgct tgctgtagca
     5581 ttgattggtc tagcactagc aatgatcagt tataagaatg atcaaaacaa agaccatcaa
     5641 ccattagcta gtgagccatc acattctgtt gagggggaaa tcgaagatga cgaaatctaa
     5701 ttacaaatta acaaaagaag attttaagca aatcaatcga agaagtttgt tcacttttca
     5761 attaggttgg aactatgaga gaatgcaggg ttctggctat ttatatacta tcttgccaca
     5821 actaagaaaa atatatggag acaatacacc agaattaaaa gagatgatga aaacacatac
     5881 gcaatttttt aatacttcta atttttttaa tacaatcatt actgggattg atcttgcaat
     5941 ggaagaaaaa gagggaattg cttcaaagga aaccgtatcg gggctgaaag ctggattgat
     6001 gggttccttt gcagcgattg gagattccgt atttgctgct ttgattccaa cgatttttgg
     6061 ggctttggcg gcgaatatgg caactgatgg aaatccgata ggtgtgttta tatggatttt
     6121 tgctcagatc gttgtcatgg tttttcgttg gaagcaactt gaatttgcat acaaagaagg
     6181 aatttcctta gttactacta tgcaatctcg cttaactgca ttaacggacg cagccacttt
     6241 attaggggta ttcatggtag gggcattagt tgcgacaatg atcaatgtta aattttcatg
     6301 ggcaccaacg attggcgatg tgacattgaa tatgcaaaac tatatcgata tgatcttacc
     6361 taaaatttta cctgctggga tcgtcggtgg tgtttattgg ttattaggaa agaaaaacat
     6421 gactcctact cgagcgattt ttatcgtatt aattgtgtgt gttgctttat ctgcattggg
     6481 tgtgatttcg aaataaggga gagcgaaaaa tgagtagaca attggtattg attagtcacg
     6541 gaaaattttg tgaggaattg aaaaaaagta cagaaatgat catggggcct caagaggata
     6601 tctacacgtt acctttatta ccagaagatg gcactgaaac atacaaacaa aagtttctca
     6661 ctgtcacaga ttcgttaaat gattttgtgg tactatgcga tcttttaggt ggtacacctg
     6721 caaatatcat ttctaaacta ataatggaag gacaacagat tactctttat gctgggatga
     6781 atcttccgat ggtcattgaa tttatcaact cacaaatgat tggttcagag cccgagtttg
     6841 ttcgtgcagg atgtgaaagc attgtctctg tcaacgaatt aattaatgga atagcagaag
     6901 aggaagagta acaaaaaaga tgagggctta gctctcatct tttttgtcct atgtttttat
     6961 ttccgcttct gctgctttcc tgcgttgcgg cctttgttgt ttgtactgtt ttttggttgt
     7021 ttgatttgtt ttgttgcttg tttgttgtta gctggctttg ctgattctgt cacgtctttt
     7081 cttggagtga cgacttgttt tggcgggttt tgtttcaact cttctgccac ttgtgctttc
     7141 aaacgtggtt tcatcataac gtttgtgatg aatgtttgca gacagctgaa cacaccaccg
     7201 actacccagt aaagtgtaac accggctggc gcactgatcg acatgaagac gatcatagca
     7261 ggagagacga ttagcatcgt acgcatcgtc tttttctgtt cttcaggaat accgatcgtt
     7321 gagatgtatc cttgaagcag ataggcgacc cctgcaatga caacaagtac catacttggt
     7381 gagcctaaat cgatgccgta gaaagatgat tcccgaatac cttctgtata acgtgccgta
     7441 aagtacaagg cagagaagat cggcatttgg atcaatagag gcagacagcc gattccgccg
     7501 gtcatactca atccgttttc gcggtatact tgctgcattt ctgcttgtgc tgccatttgt
     7561 tcttcacgtg ttgttgcagc tttcaatttt tgttgggctg cttcaacttg tggttttaac
     7621 gcttgcattt tttcagattg gatcattgtt tttttcgatt gactaatacc tagtggcaag
     7681 atgatgatac ggacgatcac agtcattgca ataactgccc agccataaga ccaattgaaa
     7741 ttctcaacta gatatgtaat cgcttggccc ataggatgga cgagtactcg ataaacgatt
     7801 cccgaagtgt cagggtttcc gtttgaatcc atccgcacac agccagataa aaagagcagg
     7861 atactgacaa gtccggatcc caataaccag tttttccatt tattcataga atacttcctc
     7921 ttctataatt tttacacaa
//
LOCUS       DANKHJ010000115         7901 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000115 DANKHJ010000000
VERSION     DANKHJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(39..1586)
                     /locus_tag="PW113_002803"
     CDS             complement(39..1586)
                     /locus_tag="PW113_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL2527059.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(1670..2473)
                     /locus_tag="PW113_002804"
     CDS             complement(1670..2473)
                     /locus_tag="PW113_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2527060.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(2476..3258)
                     /gene="phnE"
                     /locus_tag="PW113_002805"
     CDS             complement(2476..3258)
                     /gene="phnE"
                     /locus_tag="PW113_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2527061.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(3255..4034)
                     /gene="phnE"
                     /locus_tag="PW113_002806"
     CDS             complement(3255..4034)
                     /gene="phnE"
                     /locus_tag="PW113_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL2527062.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(4006..4797)
                     /gene="phnC"
                     /locus_tag="PW113_002807"
     CDS             complement(4006..4797)
                     /gene="phnC"
                     /locus_tag="PW113_002807"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL2527063.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(4858..5787)
                     /locus_tag="PW113_002808"
     CDS             complement(4858..5787)
                     /locus_tag="PW113_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2527064.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            5982..6665
                     /locus_tag="PW113_002809"
     CDS             5982..6665
                     /locus_tag="PW113_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL2527065.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            6826..7869
                     /locus_tag="PW113_002810"
     CDS             6826..7869
                     /locus_tag="PW113_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2527066.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2382 a         1664 c         1559 g         2296 t
ORIGIN      
        1 aaaactgctc acaccttttt gaatttttta tttatatact acaaaatcac ttgaaaatta
       61 tgattcactt ttgcttcgat gcgtgggtga atattcaaat agtcggcaat taattcggtc
      121 atgtcgattt ggatttctcg gacaattttc tccgcaccaa acatcgcata atttcctccg
      181 ccgattgccc gatattgatt tgtcaccact tccagcattt gttgcggaag aacggggtga
      241 cctttaaaac gaagttcggt aatccgttgt ccaacaggct gtttcatatc aatagtgtac
      301 tgaatccctt catacatgtc ataattgtag tactggggct taggctgaat aaaggctgga
      361 ttgaatacga tttggttatt ttctacagct aaataatttg cggtctgctc caacgccgct
      421 ctcaaatctg caccatttat ttgaatcact gccagagtat tcggataaat atagttggtg
      481 atgacgtccc gcatcgtgat cgtctcacca aagccttttc cttcattatt gaataacgca
      541 gttccagaaa tctctgagcc gctggcaaat aattgtacac gattaataaa ctctacatag
      601 ggatgttcct caagacgagc ctgcatcgga tcattaatac gcatatttcc gttcacttta
      661 cctaagggtt gatccaacca tttctctagt ttttcttcta ccggtacgat cgctgcaaga
      721 atttctggat caggctctga tttttcaact gagtgtaaag caacctggtg atctgtaaca
      781 accacttgcc cctctttttt ctcgacggta agacgaattt caccgatgaa ctcgccacga
      841 tagcctggtt gaacaacggg aactccatga accgtttcag caattttccg gtgctgatga
      901 ccggtgaaaa gggcgtcaat gccttcaacc tgctgcaata gctcgtagcc ttcattctct
      961 ccagttagcg cttctgtgtc ctcaccggtt aacaagtctt tttcaaatcc gccgtggtaa
     1021 ctaacgatta ccaagtctgc ttgtttcctt aatagaggaa catattcttt tgctgtttcg
     1081 acgatactct taaacgtaat tcctgctaag gtttctggct tttcccagtg aggaatgtat
     1141 tgtgtcgtta atcccaagat agcaattttt acacccacct tttcgataat cttataggcg
     1201 tttccaaagt atggttggtc atttttgttc accatattgg ctcctaatat aggcgcttga
     1261 taattcttaa tcgcttcctc gagataatcc acaccatagt taaactcatg atttcccaac
     1321 acttgaacat catagctcat ttggttaatc aagtctgtaa gctctttggc tgaaccatat
     1381 tctttagcaa gataataact caaaggagac ccttggatga aatcaccatt ttcgatcgta
     1441 attaccggtc catctgcttt tgcccgtaat tccttcagtt tcgacgatac tttagccgtt
     1501 cctaaggcca gatcggttct gccggaatag tctgttggtg aaacatagcc atgcatatca
     1561 cttgtctcta aaaacgtaat gttcattctg cgtgctcctt tagctgactc aaaatagaaa
     1621 aaccgaagcg tattcagctg ttcggtctct ttttttgatt tataatgcct taatccttca
     1681 tttgaagtgt ttttaaaatg ttgcctggaa taaagttttc tgttgtctgg cgaacaatcc
     1741 ttccttgatc gagatgataa aggttatacc catgacgttc ttcaaagcgt acgaagccat
     1801 cgccttcatc ttcaccaaca aaggacatac tcgatacagt aaaatagggg atcccattaa
     1861 tttcatcagt aaatgtgtga tgggtatgac cgtttaggat acagcgaata tcgtaaggtg
     1921 cgattagctt caagaaacgt tcggtaccag gccattttgg tatactgctt tggtgagaca
     1981 gtagatgatg atgggtgacc aagaaaatcg gttggtcttt ttcttgctct aaggcttttt
     2041 tcagccattg aaattgctga tcgtccacta taccatctgc gtatccatag cttgaattat
     2101 caaaggaaat cactgtaaac tcaggatatt tgactatctg gttgtacggg gcagaagagg
     2161 cggcttcatc gcaccagcct acccaaaaat gttctttgat atcgtgattg cctagagtaa
     2221 caagaatcgt cgtttctcct agcgcatttt ccagccaagt cttcaagtat cgataatcgt
     2281 ctatttcgcc atcttcggtt aaatctccgc tgataatcac aaggtccaag ggtgtttgct
     2341 gcaggagatg atgaatgcat tgctccagag ggataagggg attctgcatc gtagccagca
     2401 ttcccttata gccttcttca caagcctcat actctctgcg aaagtgtata tctgatatat
     2461 gcaatatatt cattcttatc tcaatctctt tcttataatt ccggttattt gatctaaaat
     2521 aaacacggtt acgactacgc caatcaaaat aatactcact ttgctccaag accgagtttg
     2581 aatcgcgaat atcaatggag caccgattcc gccagcgcca actaagccta aggtagccgc
     2641 actacgaacc gctatttcaa aatgatttaa tgctaaagaa caataaatcg gcatcaaata
     2701 ggggatgcga acaaagaaaa tcgtttgcca aaaatttgct cctaccgcat gcatttcttc
     2761 aacaagtcgc tcatccatcg cttccatttc ttctgtaaaa agtttgccaa gcattccgat
     2821 ctgatggacg ccaatcgcca taacaccagc aaaggggcca ggtccgacga ctttcacaaa
     2881 aataattgca aaaactagtt caggaaaagc tcgtaaaaca ttacagataa gcttgccaat
     2941 ttttgttacc catggatgtg cttgccagag gttaaccgca ctgatcaagg taatgggaat
     3001 cgctaatacc gtagcgatca ttgtccctaa acaggcgatt ccaatggtta ataatagcaa
     3061 actaatcaaa tcctcgccgc tgccatcata tagaaactgc caatcaggct gacctaaccc
     3121 tctcaaaacg tccattccca tggaaggaga gaaggtgctt aatccagagt aatccagagt
     3181 agcggccgac aaaataacga gaatcactaa agcaactaat gataaaaggg ttcttctgtt
     3241 catttcacga agcatcataa cagggacctc ctgacacgtt cacttaattt atcaacgatc
     3301 gcgacagtta ctaggatcgt tagaataatg attgaaactc gttcataatt caaaagcgca
     3361 agtgaggaat ttagaataac gccaatacca ccagctccga catatccaag gacagtagaa
     3421 gcacgaacat tcacctcaaa cgcgtaaaaa gcatagctcg caaactgact caaaacttgt
     3481 ggagcgatcg accaaatagc gatttgcatt ttatttgctc caacagccgc agcggcttca
     3541 atcgggccaa agtcaatggt ttcaattgct tgaaacatta attgtgacac cataccaaaa
     3601 gtaaagacag cgatagtcaa cacgccagtc gcttcaccaa ttccaaacac agcaacaaaa
     3661 agcgcggcta aaagcagggt agggatggtc cgtactaaac ctaaaagaaa acgaaagatg
     3721 gtagaaagaa gacggttttc tgtcactaca gtggtcgcta aaaaactaac agggatcgct
     3781 aaaattaagc caatgaccgt tccaagcaaa gacatttgaa gtgttttcac cataggctcg
     3841 actaatttag gtaaatagct aaagtctgga tgaatgaagc gtgataaaaa gacagtcatt
     3901 tgattggagt tttgaaaaac ctgagaaaaa tcagctgacg tgatccaggc acttccttga
     3961 aaacaaacaa ttaagaacag aagaataagc aggtgtttat accatttaaa gtgaacggtt
     4021 tctttcaagc tcatcgattt cgcctgcctt ttcttcaaag attgcattgc cataaatttg
     4081 ctctaagcgt tcatcggtta agtcgtctgg acgaccatca aagacaacct gagcagcttt
     4141 taacgcgagc acacgcgtag aaaactcacg agccagaggg actgaatgca gattgacaat
     4201 gactgtttta tccatcgttt gattgatatt ttttaggtcc atcataacct tttgagtcgt
     4261 gattggatct aatgatgcaa cgggttcatc cgctaaaata atgggggctt gctgcacgag
     4321 tgctcgcgca atggatactc gctgttgttg cccaccactg agctcatcac tgcgtgtctg
     4381 caatttatca gacaaaccaa ctttttccag tgcttccttt gtctgctggt agtcttcctt
     4441 cgtaaaaaga cctaaaatac ttttccaagt ggagtaatag cccaaacgtc cagatagcac
     4501 atttttctga accgtactac gctttaccag attgaagctt tgcgagatta gaccaatctg
     4561 gcggcgaatc tgtcgcagct cttttttatt cgcttttgta atagaggttc cattaatgcg
     4621 aatatctcct ttggtggttt ctgccaagcg attgatactg cgcagcagag tgctctttcc
     4681 agcaccactc aaccctatga tggaaacaaa ttctccatct tcaattgtta agtcaatatc
     4741 cttcaagcca acaacgccat tgggatagac cttcgaaaca ttgtcaaaaa taatcattat
     4801 ccttctccat ttttttaagt gtaagcaaaa aaaagcttgc tgacacttac tgatgtttta
     4861 ttctgctgct tttttcgtgt agtctctgat cgtgtcatag gcagaatcag cagcttcttc
     4921 atagccctga tgcccccaca aagccattgc ttctttgcca tctttatcat ccttcatatt
     4981 taagaaggtc tctttaattt tcgcttttaa atcttttgac atgtcaggct gtacagcgat
     5041 tgcgtcattc ggaatatcgc cttttgtcag gtacaggact tttaaatcct taaataaatc
     5101 attatctggg aaattcgaag caaaaacatt tcgcgcgcct tcaaaaacaa acgccgcatc
     5161 catttgcttg ttcaacactg cggtaatctc actcggaata tcattcacag tggtcatctt
     5221 tgtatctgca gtaggatcaa tcccagcatc tttcaattca gcgacaggat agatgtagcc
     5281 gctagcagag ttaggactta aggttgcgat attttttcct tttaaatcag ttaacttatt
     5341 caatccgcta tcggcacgaa ccaatacttc tcctttaaag gtgttcgtaa gtttgtcctt
     5401 taatggcaag ccagtttctt gatcataatc gccgagctga gaagtcagga ttgcatctgc
     5461 agctccagca tcctttgctt gaacataggc tgcaggcggc ataattccga catcgacttg
     5521 accagaagac atcgcttcga cgatcgttga ataatcagta gctaacgtca cttctacctc
     5581 acgatctaac tttttcgata aatacttagc aaatggtttt gcttttgcct ccatactacc
     5641 atcattgttt gttggaacga attgaacaac taatttttct ttgctctctt tttctgagct
     5701 gctacttgta ttcccagaac cgcaggccgc caaagtggtc aataacatga ctccgatgaa
     5761 cccttttgct aactgtttga actgcatttt tctcctcctg ttggatataa tttctaatac
     5821 gtctaaatca tagcagggaa tcaaaatcaa tgaagtgacg cacgtcctat tttacacaac
     5881 ttttacatac aatttacaaa caattataaa taattattcg tattgagccg aaaaacaatc
     5941 taagctataa taaagaaaaa cttcttagag gtgacgattt catgaaaaaa atccatgata
     6001 cgaagcaatt aactgcttac gttgagaagc acaacctgca ggattatatg gattcagata
     6061 tattggcaat ggcttcttta cttttttttg aaaaggaaga gcacctcatt caaacgggaa
     6121 aaacctcaga ttatttgtat tttttagtgg atgggacggt aatggtgtac tcgtattctt
     6181 ctgatacaca aaatatttgc atcgattacg caaagcccgc tacaccactc ggcgaagcct
     6241 cttcattgtg ggaattatcg ccgaaaagca gcgttaaagc agtgactaag tgcatctgta
     6301 tcagcattcc gctaaatcaa tatcgaacac agcttcaaaa agatgttcgc tttttgcaga
     6361 atatttgcca gttactaagt tatcgtttaa attccgggat aaatttggcg aactcactga
     6421 cagaacctgt tgaaactcgt ttagcaaagt ttattttgac ccatcacgaa aacgaacgtt
     6481 tctcatttcg gttaaccacc tgcgcctcaa tcttaaatgt cagttatcga catcttttac
     6541 gaacaatcac gaattttcgt gaggagaata ttatcgaaaa gaaaaagaat tattacttga
     6601 ttcatgatat cgaagcttta gaggacttag ctgagaatat aagtagtcca gtaaaagcag
     6661 aataacagct ataaaaaaac agcagtcaga agtgattgct ggttttttga tagtggagaa
     6721 attgggaaac ggaaaatctt cgttgaagag tagtgttcta tatattggta tgctatactt
     6781 ttaaaagttc aatgtgtcaa gacacataaa aaggggagaa aaattttgaa gaaaatactt
     6841 gtttcattgt tgtttttggt gtcattagta acgttagtcg gctgtagtca gtctcagggg
     6901 gagaagcaaa gtgaaaaaga gaaaagtaaa atcgctttgg ttcttggatt tggcggggtt
     6961 gatgaccgct ccttcaatca agctggctgg gaaggattag aggcttgggg aaaagagaaa
     7021 aagctgacag caaaaaaaga ttttacgtat atagaaacga acaatccaga ggataacagt
     7081 accaatatcg atcaggcaat cagaaacggc tttaccacca ttattagctt gagctacttg
     7141 caagcagagg aactggaaaa tgctgctaaa gataatccaa agttgaaatt tgccttggta
     7201 gatggtgaga ttactgggaa aaagaatatc tcatcagcaa cctttaaaga tcaagaaagt
     7261 gcgtatttag caggactagc tgccgcatat acgacaaaaa gtgatcaagt aggctttatt
     7321 ggtggaatgg aagggcccgt gatccaacgt ttccaaagcg gatttgaagc tggcgttaag
     7381 gcaggggcca cagcactgaa caaggaaatt actgttaacg tacaatatgc tgcatcctat
     7441 gattcgctag ataaaggaaa agctattgct gcttctatgt acaaagaggg agtcgatatt
     7501 ctttatcagg cggcaggaag tactggagct ggggtgttcc aagaagcaaa agcccgcaat
     7561 gaaaacgact atagcagaaa agtatgggtg attggtgtag atcgcgatca agcggatgaa
     7621 ggggattata aaatggagaa cggcgagaca gggaatttca cgttgacttc tacaaagaaa
     7681 aatgtcggtg cagaagtcaa agaatttgca gaaacttctc ataacgataa atttcctggc
     7741 ggcaagcatt tgacctttgg tttgaaagaa gaaggggtag atctgacaaa aggacaatta
     7801 tcagaggaag cctatcaggc agtttcagac gcaaaagaga aaatcatcag tggtgatttc
     7861 agactgtaga caaagtccat tttatggatg atgtctacag t
//
LOCUS       DANKHJ010000116         7886 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000116 DANKHJ010000000
VERSION     DANKHJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            2..74
                     /locus_tag="PW113_002811"
     tRNA            2..74
                     /locus_tag="PW113_002811"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="PW113_002812"
     tRNA            78..150
                     /locus_tag="PW113_002812"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="PW113_002813"
     tRNA            155..225
                     /locus_tag="PW113_002813"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="PW113_002814"
     tRNA            238..311
                     /locus_tag="PW113_002814"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="PW113_002815"
     tRNA            323..394
                     /locus_tag="PW113_002815"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="PW113_002816"
     CDS             660..1853
                     /locus_tag="PW113_002816"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2527067.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="PW113_002817"
     CDS             1983..3455
                     /locus_tag="PW113_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2527068.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSCDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="PW113_002818"
     CDS             3538..4326
                     /locus_tag="PW113_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2527069.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="PW113_002819"
     CDS             complement(4393..5061)
                     /locus_tag="PW113_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2527070.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="PW113_002820"
     CDS             5176..6888
                     /locus_tag="PW113_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527071.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..>7886
                     /locus_tag="PW113_002821"
     CDS             6891..>7886
                     /locus_tag="PW113_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527072.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEK"
BASE COUNT         2335 a         1356 c         1756 g         2439 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatcttg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaa
//
LOCUS       DANKHJ010000117         7859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000117 DANKHJ010000000
VERSION     DANKHJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            278..787
                     /locus_tag="PW113_002822"
     CDS             278..787
                     /locus_tag="PW113_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2527073.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PW113_002823"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PW113_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2527074.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PW113_002824"
     CDS             2322..3302
                     /locus_tag="PW113_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2527075.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PW113_002825"
     CDS             3424..4842
                     /locus_tag="PW113_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2527076.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PW113_002826"
     CDS             4913..6382
                     /locus_tag="PW113_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2527077.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PW113_002827"
     CDS             6392..6859
                     /locus_tag="PW113_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2527078.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PW113_002828"
     CDS             6895..7767
                     /locus_tag="PW113_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2527079.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2689 a         1183 c         1603 g         2384 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaa
//
LOCUS       DANKHJ010000118         7793 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000118 DANKHJ010000000
VERSION     DANKHJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(148..1440)
                     /locus_tag="PW113_002829"
     CDS             complement(148..1440)
                     /locus_tag="PW113_002829"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2527080.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(2220..3587)
                     /gene="dnaB"
                     /locus_tag="PW113_002830"
     CDS             complement(2220..3587)
                     /gene="dnaB"
                     /locus_tag="PW113_002830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2527081.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            complement(3861..4313)
                     /gene="rplI"
                     /locus_tag="PW113_002831"
     CDS             complement(3861..4313)
                     /gene="rplI"
                     /locus_tag="PW113_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2527082.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            complement(4319..6292)
                     /locus_tag="PW113_002832"
     CDS             complement(4319..6292)
                     /locus_tag="PW113_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2527083.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            complement(6435..6671)
                     /locus_tag="PW113_002833"
     CDS             complement(6435..6671)
                     /locus_tag="PW113_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2527084.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            complement(6697..7209)
                     /gene="ssb"
                     /locus_tag="PW113_002834"
     CDS             complement(6697..7209)
                     /gene="ssb"
                     /locus_tag="PW113_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2527085.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            complement(7259..7558)
                     /locus_tag="PW113_002835"
     CDS             complement(7259..7558)
                     /locus_tag="PW113_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2527086.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
BASE COUNT         2154 a         1650 c         1395 g         2594 t
ORIGIN      
        1 ttctgaataa acagtgttca agagacagcc tatcggcaat aaggctaaac gtctctttca
       61 ctacctcttc aagttattat tatcgtatag ataaacactt cgactcgctc tggtaagaac
      121 gtcctgattc agtcgtcgtt tttatcctta gatttgcgcc cagacgcttt ctaagatgtt
      181 tgtttgtgtt ctatcaggtc cgacagaaaa tgttgagata cgtacaccaa caagctctga
      241 gatacgacgt acatagttac gtgcgttttc tggcagttct gcaagtgttt tgcatcctgt
      301 aatgtcttct gaccagcctg gcaattcttc atagattggt ttgcagcggt tcaactcttt
      361 caagcttgct ggatagtggt agattagttc gccatctaat tcataagccg tacaaatttt
      421 tactgtttcc aatccactta atacatcgat cgagtttaaa gataggttgg taatacctga
      481 aactcgttta gaatggcgca taactacaga atcaaaccag cctacacgac gcggacgacc
      541 agttgtcgta ccgtattctc gtcctacttc gcggatttgt tctcctgttt catcaaataa
      601 ttcagttgga aatggtccat caccgacacg tgacgtatag gctttgcaga cgccgacaac
      661 tttattgatt tttgaaggcc ctacaccgct tccgattgtt accccaccgg ctactgggtt
      721 agaagaagta acaaaaggat aagttccctg gtcgatatcc agcattacac cttgtgcgcc
      781 ttcaaacaac acgcgtttgc ctgcatccaa agcatcattc aagataacgg aagtatctgt
      841 gacgtattgt ttgatttgtt gtccgtattc ataatattct tcaaaaatat cgtcaaaatc
      901 aagtggttct gaatcaaaca tttttacaaa ttgacggttt ttgtcatcta gattcaaacg
      961 aagacgttct tcaaaaattt ctttatctaa taagtctgca atacggatac ctacacgagc
     1021 tgctttatcc atataggcag gtccgattcc tttaatcgtt gtaccaatct tattttcgcc
     1081 cttagcatct tcttgtaatt ggtccaattt gatatgataa ggcaagatta cgtgcgcgcg
     1141 gtcagaaata cgcaaattac ttgtttctac acctttttca tgtaaataag ctaattcttt
     1201 aaccaatgat ttaggattga ctacaacgcc gtttccgatc acactgatct tgtctttata
     1261 aaaaatccca gaaggaatca aatgcagttt ataagtcaca ccgttaaact taattgtgtg
     1321 ccctgcattg tcgccacctt ggtaccgtgc aatcacttca gcattttcac tgagaaagtc
     1381 tgtgattttt cctttccctt catcgcccca ttgcgtacct actactacta cagatgacat
     1441 gtgaacacct cgttctatat tatttaatcc tgaaccattg tatcaataat agatacatat
     1501 ttcaaataaa atcgaatgat ttttattttt attttataaa aacgcttaaa aaacgaactt
     1561 tatcttttta gaatggttat taaaaaagag gttgtaacag aagtggtcag cttcaagaaa
     1621 taagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatttcgac ttatttttcg
     1681 aagaagctac ttttggaaca ccgtgaatca gaggttgtga aaaagctgtc ctgcatcaag
     1741 taataagaac agccaaacaa aaatagcttc tcatattttt cgtttgattg ggcgtattac
     1801 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgagcctgc cgtttaactc
     1861 tgagtcacaa ggagcaattt caaaaaactg cttctcatgt ttgatgatat tgtgacttgt
     1921 gaccgaggag ttggcaggag aacaccgttt aaggaaaaga ggtcgtaaga tcagcgtttt
     1981 gacctgagta ttagagaaac aaacgtcaaa atagttcctc atattttgac gtttgctgag
     2041 ctaataccaa gggtcagctg attgaacacc gtttattcgg aaaaagggac tgtgacaaga
     2101 cttttgtcac aatccctttg gagatctgtt taaattaagt actctagtcg ttcctgcggt
     2161 tactccttgc atcttcttac gagagctctc gggattgctc aaggctcaca acctcttctt
     2221 taaaattctt ctcttggcga aagcgacgaa aacttattgt attctttgat aaacagtagt
     2281 tcaactgttc ctcgtgcacc gcttcggttc ttctcgatga tgacttcgat gacgttgtca
     2341 ttttttggct cgtttccatc atcgtcatct ccgccttctc tttcataata atcatctcga
     2401 tacaagaagg ctacgatgtc cgcatcttgt tcaattgatc cggattcacg aatatcactt
     2461 agcactggtc gtttgtcttg tcgctgttct actccacgag ataactgtga aagagcaatg
     2521 acaggtactt tcagctcttt cgccagtttt ttcagttggc gagagatatc tgatacttct
     2581 tgttgacggt tctctttacc gttaccttca atcaactgca aataatcgat caatatcaag
     2641 cctagattgc ctttttcttg cgctagtttc cgacatttcg cacggatttc agagattttg
     2701 atccctggtg tatcatcgat aaaaatattt gctcgagaaa gactccccat cgcaacgatc
     2761 agattcgacc attcttcttc cgaaagctga cctgtacgta aatggctggc ctcaatcgaa
     2821 ccttctgcac aaagcatccg atttacaaga gattctgccc ccatttccaa actaaatatc
     2881 gcaacagaac gatctgtttt tgttcctaca ttttgagcaa tattcaatgc aaacgctgtt
     2941 ttacctaccg ctggacgtgc agcaagaatg attagttctt cgggctggaa acctgctgtc
     3001 attttatcca gaccttgata gccagttggt agacctgtga tttcttcatt gttttgagct
     3061 agtcgatcga tattttcaat cgtggagttc agtacttcag caatagataa aaagccgctt
     3121 cgatttcttt tttccgaaac ctccaagata ctgcgttcgg catcatctaa aattgtttga
     3181 acttcttctc cttgctcgaa tcctttcgta acgattcctg ttgctgtctg gatcaatgta
     3241 cgcagcaatg atttttcttc aacgattttc gcatagtaga cgatatttgc tgctgtcgga
     3301 actgctaatg ccaagtctga gagataactt aaaccaccgg catcttctaa caaattggct
     3361 tgttccagcc ggtctttgac agtaaccacg tcaatcgctt cattacgatc atttaactcg
     3421 atcattgtct caaaaataat ttgatggcca cgacgataaa agtcttttgg ctctatgtac
     3481 tccatggcat caatgatcgt ttctgcgtca agaaaaacag agcctaaaac cgcttgttca
     3541 gcttcaatat tttgcggtgg gattcgatcc tgccacactt cattcatacg tatcgctcct
     3601 tgtcttcaat tgcttcattt tacaagaaaa tgagagaaaa gtcatcattc gtaccgaaag
     3661 tgtaataaaa aaatagttct cttttattat actcgttttc tttatgctat tttgctgttc
     3721 ccaaaaaaga aaccgaaatc aatctgaaat cgatttgcag cttttgcttt cattgctgca
     3781 gctgttttct cagataaatt tcggtttgtc ttttttcctt ctggttatac ttaccagctg
     3841 aacatcgcta tcaagatttg ttattcttgt acgacatgaa ctttgatcgt agccgtcacc
     3901 tcatgatgaa gcttaacagg tacgtttgtg tagcctagtg aacggatcgg attttctaat
     3961 tcgatttttc gtttatctaa tttgattttg tattgtttgt ttaacccttc agcaatttgt
     4021 tttgaaggga tcgaaccaaa caaacggctg tcttcccctg ctttcgcctt caattcgact
     4081 acagtatctt ctttttccaa gaattctttt aactttttag cttcttccaa gatttcagct
     4141 tcgtgttttt cttgtgcttt cttttgtcct gctaactgac tgatactgct tttcgttgct
     4201 tcttttgcca agttattttt gattagataa ttttgtgcat aacctgctgg aacttctttg
     4261 atgtcccctt ttttaccttt tccttttaca tcttgtaaaa aaattacttt catttggatt
     4321 actccttatc atatatttga ttcatatttt ctttgattgc agcaaatagc ttctgttttg
     4381 tttcctcaac tgtcgtattt tccagttgag tagctgcgtt tgtgaagtgt cctccgccac
     4441 ctaaggattc catgatgatt tggacgttga tcgtgccggt acttctggca ctgatcccaa
     4501 tcaagccgtc tgtccgcttt gtaatgacga aagctgcatt gatattgatc atcgaaagca
     4561 atgtatctgc tgtttttgct gctataacac tattatactc tttttcgtct gtccctgccg
     4621 ccacaacaat atcagaagtc acatagtcgc ttccagcaat caaattgctg atctccaagt
     4681 atgtcgctaa atccgagctc aataaatatt gtactaagga tgcgtctgct ccacaagacc
     4741 ttaaatagct ggcaacgtca aaagttcgtg cagttgtacg gacattgaaa ctatttgtat
     4801 ctacaaccat ccctgcaagc aataatgtgg cctcaaaacg atctaagcga tttgttttgc
     4861 tgctttggta ttggatcagc tcagaaacta gctctgaagc tgaagaagct gaagattcaa
     4921 tataagtcag cagaggttta cttggaaact cttccccacg acgatggtga tcaatgatca
     4981 caatcttatc aaattgctca tataggccct gagagatcga tagagaaggt ttatggtaat
     5041 ccaccatcac gaggaggtcc tcttcttttt tcagctctat ggcacgttct ggagagatta
     5101 gttgcatttc taattcagga tgcttatgga tttcttcaag acaccgttct acatctggaa
     5161 tgatctcgtt ctcattgata acgatgtaag cttttttctg gttgaagcgt gccagacaac
     5221 tgacaccaaa agcagagcct aaagcatcca tatccggaaa cttgtgcccc atgataaaca
     5281 catcgcctgt ttcatcaaaa atccgacgca aagcggtact catcgctcga gaacgaacac
     5341 gcgtgcgttt gacattctta gcagatttcc ccccataata aacaggtttt gctccttctt
     5401 ttacctcttt cacgacaact tggtcgcctc cacggactaa agcaacatct aaattattct
     5461 gagcttctcc accgatttca tcgagggttc ttgagccata agcaatcccc atactaaggg
     5521 tgataggcga gctctgtttt tcagcttctg ctcgaaagcg atccagcaaa tcaaattttt
     5581 tatccatcat ctttttcaga tcgatcatct gtgctacgaa gaaaaaccgt tctgcgttga
     5641 ttcttttgaa aaagacatgg tattcattca tccagtcaga aactagtgta gtgacgaaag
     5701 agttcaaata agaaatctct ttgtcatcta atttatcaat ggcatctgca tagttatcaa
     5761 tagataaaat accaatcacc ggctgactgt tcgtctgatg gagttttaac aactggtttt
     5821 gggtaatatc attaaaataa acaacaccct ttactgtatc catatcaaac tgaaatgttt
     5881 tatctgctag atagacttgg tttttctttt tttcttttgc agtaaaaaaa gtatctagca
     5941 attcttcctc tgttaatcca ctttgatctt tgattttcaa tgctaatgga ttgagccatt
     6001 cgattttttg gcttgatggc tgataagcaa tcactcctac aggcatgact tctgagacat
     6061 aagctagatt ggtttcagct acagtcgaag cctgctggat tttttcacgt ttatttaaaa
     6121 taagccaacg tttcattttc aaccaatagc gaacacccca taaattcatc aatagaatga
     6181 ccgaagcgct aacaaaacga aatggtaaaa ttaagactac caatacttcg attaatataa
     6241 acaggataag gaagagaggt ttcggggata aatgattgtt tttattttcc ataattttcc
     6301 tcccatatat gactaatttg attttaccat aactttcgtc attcgagcga ctaaacgaat
     6361 agaggttatt tttacagaaa aaaaggaatt gaatagctga tcaaaaggat cagtcattca
     6421 attcctagga tttattagtc ttcgctaacg aaaggtagca agcccatgat acgagcgcgt
     6481 ttgattgcaa cagtaatttt acgttggttt ttagcgcctg taccagttac acgacgtggc
     6541 aagattttac cgcgttctga aataaatctt tttagtaatt cgatatcttt ataatcgata
     6601 tattcaatat ggttagcagc aatgtagtct actttacgac gtttacgtcc gcctcttctt
     6661 tgttgtgcca ttctatttcc ctcctatcaa attcgtttag aatggtaaat catcgtctga
     6721 aatgtcgatc gttgaacttg agccgaatgg gtcagaagta tcacggtcga aatcaggcat
     6781 accgttggat gattgagatg tttgattaga gttattgttt ccgccaaatc cgccagctga
     6841 ttgaccagaa ctttggaatc cgccgctttg ttggcgattt tcagatgcag aacgactttc
     6901 cagcaattgg aagttgtcag ctactacttc tgtcacgtat acacgttgtc cttgctgatt
     6961 atcataagaa cgagtttgga tacgtccagt aacacctaaa agtgttcctt tacgagcata
     7021 gtttgctaaa gtttctgctg atttgcgcca aatcacacag ttgataaaat cagcctcgcg
     7081 attgccgctt tggtttgtaa aattacgatt cacagccaaa gtaaatgttg cgactgccgt
     7141 accacttgct gtgtaacgca aatctggatc tttcgtcaga cgtccaacta atacaacatt
     7201 attaatcaaa atagtcatct cctctcatcg ttttctattg tttcacatga aacaacaatt
     7261 aagcttcttc tttaacaatc atgtgacgaa tgatatcgtc gttgatttta gcaagacggt
     7321 caaattcatt gacagcattt gctgatgatg gagaagatac tttaacgatg tgatagatac
     7381 cttcacggta accgttcatt tcataagcga aacggcgttt ttcccaatct tttgattcga
     7441 taacttctgc tccgttatct ttcaagattg catcgaaacg ttcgattaaa gcagtttttg
     7501 cttcttcatc aatgtttgga cgaataatgt acatgatttc atatttcgtg ttttccattg
     7561 atattcacct ccctgtggac tttcggctct taggttccta agagcaagga gagttatcta
     7621 attaattaga ctactcacaa gctctaatta tacactctat tttggtcaaa agcaatacag
     7681 aatgaacagt ttcgaatatt ttaagagaaa ttcaactaat caaaaaagcc agcaggaata
     7741 accagatttt ttaatcggtt attcttgcta gcttacacac ttagtcttga tgg
//
LOCUS       DANKHJ010000119         7647 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000119 DANKHJ010000000
VERSION     DANKHJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            17..2344
                     /locus_tag="PW113_002836"
     CDS             17..2344
                     /locus_tag="PW113_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2527087.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="PW113_002837"
     CDS             2470..2799
                     /locus_tag="PW113_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2527088.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="PW113_002838"
     CDS             2839..3843
                     /locus_tag="PW113_002838"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2527089.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="PW113_002839"
     CDS             3840..4286
                     /locus_tag="PW113_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2527090.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="PW113_002840"
     CDS             4436..5251
                     /locus_tag="PW113_002840"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2527091.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="PW113_002841"
     CDS             5457..6629
                     /locus_tag="PW113_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2527092.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7285)
                     /locus_tag="PW113_002842"
     CDS             complement(6839..7285)
                     /locus_tag="PW113_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527093.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(7282..7476)
                     /locus_tag="PW113_002843"
     CDS             complement(7282..7476)
                     /locus_tag="PW113_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527094.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2574 a         1444 c         1525 g         2104 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7081 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7141 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7201 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7261 acaaagcaga ttccgcttct gtcacttttt ttctagtgca gctttttccg ctttttgttt
     7321 ctcttctttt ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga
     7381 gatcgtggta ctgctgctgg aagcctgatc tgaacttgtg ctttgagtag tagacgtagt
     7441 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aatgcaacta attttttcgc
     7501 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     7561 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     7621 gttgatacac gggatttttt gctatag
//
LOCUS       DANKHJ010000120         7600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000120 DANKHJ010000000
VERSION     DANKHJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(943..1356)
                     /locus_tag="PW113_002844"
     CDS             complement(943..1356)
                     /locus_tag="PW113_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2527095.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="PW113_002845"
     CDS             complement(1812..2297)
                     /locus_tag="PW113_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527096.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="PW113_002846"
     CDS             complement(2436..2651)
                     /locus_tag="PW113_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2527097.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="PW113_002847"
     CDS             2875..3675
                     /locus_tag="PW113_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2527098.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="PW113_002848"
     CDS             3694..5562
                     /locus_tag="PW113_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2527099.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="PW113_002849"
     CDS             5555..6046
                     /locus_tag="PW113_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2527100.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="PW113_002850"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="PW113_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2527101.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="PW113_002851"
     CDS             6606..>7600
                     /locus_tag="PW113_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2527102.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DANKHJ010000121         7512 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000121 DANKHJ010000000
VERSION     DANKHJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..389
                     /locus_tag="PW113_002852"
     CDS             <1..389
                     /locus_tag="PW113_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2527103.1"
                     /translation="KKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFVI
                     VFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTFA
                     GKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            373..2823
                     /locus_tag="PW113_002853"
     CDS             373..2823
                     /locus_tag="PW113_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2527104.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            2828..4936
                     /locus_tag="PW113_002854"
     CDS             2828..4936
                     /locus_tag="PW113_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2527105.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            4933..5937
                     /locus_tag="PW113_002855"
     CDS             4933..5937
                     /locus_tag="PW113_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2527106.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            5956..6867
                     /locus_tag="PW113_002856"
     CDS             5956..6867
                     /locus_tag="PW113_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2527107.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            7054..7341
                     /locus_tag="PW113_002857"
                     /pseudo
     CDS             7054..7341
                     /locus_tag="PW113_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2448 a         1280 c         1623 g         2161 t
ORIGIN      
        1 tgaaaaaaat aaaaagctac accagtattt gggcagttga aaaagtcatt tatgcgatta
       61 atgactttca gctgcctttt cctgtgacct ttaaccagat ggcttggttt gttttatcct
      121 tgatgtttgt cattgtattt gcccatgtgc ctccgctatc catgatagag ggggcttttt
      181 taaaatactt gggcattccg atagcggtca cttggttcat gtcgcaaaag acctttgacg
      241 gtaagaaacc attgggattt ttacgatcct ttatcagcta tcacttacgg tttaaggtca
      301 cttttgcagg aaagaaagtt aaagaacaga agaaacgatg ggacgaacca atcaccttgg
      361 taaggagtgt gaattatgta cccgattaag tatattgaaa ataatcttgt ctttaaccaa
      421 gaaggcgagt gttttgctta ctatgaatta gtgccttaca attactcctt tttgtcacct
      481 gaacagaagt atcaagtaca tgataacttt cgccaattaa ttgcgcaaaa tcgtgaaggt
      541 aagatccatg ccttacaaat tgccacagaa agtagtattc gtgccacaca agaacgttcg
      601 aaaaaggaaa ttaccggtag actcaaagaa gtagcaaagc aacgaattga tttacagaca
      661 gatgcattag tatccatgat tggcgatagt cagattgatt atcgcttctt tattggcttt
      721 aaactgattt cgacagatga agaggtcaat ctaaagaatc tcaggaaatc tttcttttct
      781 ggacttcaag aatttgtcta tggagtgaat catcatttga tgggtgattt tgtttcctta
      841 tccaatgaag aaattcgtcg ctataccaag cttgaaaaac taatggaaag taaattggct
      901 cgaagattta aagtcaaacg agtaacgccg agtgacttga cgtatttaat tgaacatatc
      961 tatggtgaaa aaggcacacc gtttgaagaa tatgaattcc agctaccaaa gaaaaagctg
     1021 aaatcagaaa cgctggtgaa acgttacgac ttattacgtc ccactcgttg tttgattgaa
     1081 gaaaagcctc ggtacttacg gatggaacat gaaaatcatg agtcttatgt agcgtactta
     1141 acaattaata ccattgtagg ggaaatggag tttccttcct cggaactttt ctattaccag
     1201 caacaacaat ttacgtttcc gattgatact tcaatgaatg tggaaattgt gacgaataaa
     1261 aaggccctag ctaccgttcg taataagaaa aaggaattaa aagatttaga caaccatgcc
     1321 tatcaatctg ataatgaaac gaactccaat gtattagatg ctttggattc cgtggatgaa
     1381 ttggaaacca ccttggatca gtcgaaagaa tccatgtata aactaagcta tgtggttcga
     1441 gtgagtgcta aatcagtgga tgaattaaaa cgccgctgtg atgaagtgct cgatttttat
     1501 gacgatacca atgtaaaact agtccggccc tttggcgata tgatgggtct acacaaagaa
     1561 ttcttaccct caagtaagcg atacatgaat gactatattc aatatgttac gtctgacttt
     1621 ttggcgggac ttggttttgg ggctactcaa atgctaggag aattagaagg catttacttt
     1681 ggctataacg tagatacggg gcgcaatgtt tacctgaaac cggcattggc atcacaaggg
     1741 gtcaaaggct cagtaaccaa tgctttggca tctgctttcc ttggttcgtt aggtgggggg
     1801 aaatctttta gcaacaatct cttagtctat tatgccgtcc tttttggtgg gcaagctgtg
     1861 attgtcgatc caaaaggaga aagaggcggt tggaaagaaa cgttgccaga aattgcggat
     1921 gaaattaata ttcttaactt gaccagtaag ccagaaaatc aaggcttact tgatccctat
     1981 gtgattatga agaaaaagaa agactcagaa agtttggcga ttgatatcct gaccttttta
     2041 acagggattt ccagtcggga tggggagaag ttccctgtgt tacgcaaagc cattcgaacg
     2101 gttacccaaa gtgatcagcg aggattatta caagtaatcg ttgaattgcg taaagaggac
     2161 tcaccagtcg cggaaaatat cgcggatcac atcgagtcct ttaccgatta tgattttgcc
     2221 catttgcttt tctcagatgg ttcgattacc cagtcgatta gtttagataa gcagttaaat
     2281 atcattcaag tagcggattt ggttttacca gatgcagaaa ccacttttga agaatatacc
     2341 accatggaat tgctgagtgt ttccatgcta attgttattt ccacttttgc cttggacttt
     2401 atccatagca accgagaaat tttcaagatt gtggacctgg atgaggcttg gagttttctt
     2461 caagtagctc aaggaaaaac gttatcgaat aaattagtgc gtgctggtcg ggccatgaat
     2521 gccggtgttt acttcgtcac gcaaaacagt gatgacttgt tggatgaacg tttgaagaat
     2581 aatattgggc taaagtttgc atttcgcagt accgatattc atgaaatcaa gaaaaccttg
     2641 gaattctttg gattagataa agaagatgaa agtaatcaaa aatgcatgcg agatttggaa
     2701 aatgggcaat gcttgatgca ggatttgtat ggtcgagtag gtgtggtcca agttcatcca
     2761 gtctttgaag aacttttcca tgcttttgat acccgtccgc cagttcaaga gagaatggag
     2821 tgaggagatg aaaaagaaac tttttcgtat ctttggaatt gttttaatca gtttggtcac
     2881 tctgataata cttttgagct tagttggaac ggtagctgag gcaacaggtc tggttgatga
     2941 tacagttgga gccggaaatc tttattccca atattcactt ggtaactatc agttggattt
     3001 ctttgtggat agttcgtggg attggctgcc ttggaattgg ggtgatggcc tagggaagag
     3061 cgtaatgtat ggactttatg ccattactaa ttttatttgg acagttagtc tctacttatc
     3121 aaatgccacg ggctacgtgg ttcaagaagc ctataagctg gatttcattt cagatacggc
     3181 tgaaagtatt gggaagaata tccaaacctt agccggcatt accgaaaatg gactccagac
     3241 ctcgggcttt tactttgggt ttctattatt gatgatttta gctctaggag tttatgtggc
     3301 gtatacagga ttattaaaac gagaaactac caaagcagtc cgggctgtca ttaattttgt
     3361 gatgattttt ctactttcgg gttcgttcat tgcgtatgcg cccacctata tcacaaaaat
     3421 caatgatttt agctcagatg tcagcgaagc ggctcttagc cttggaaccg agattgtcgt
     3481 gccgaattca gaaagccaag gaaaggacag tgtggattta atccgagata gcttgttttc
     3541 cattcaagtc caacaaccgt ggttactctt gcaatttgat gattccaata tagaagaagt
     3601 tggcgaagat cgtgttaaca aaatcctgtc tgtaagtcca gatgaaaata aaggcaagga
     3661 tcgggaagaa gcggtcaaag cggagattga agataatgac aatgcgaatc tcagtattac
     3721 caagaccatg aaccgcttag ggaccgtggt ctttttggtg ttgttcaata ttggcatatc
     3781 cttctttgtc tttctcctaa cagggattat gttgttctcg caaattctct ttattatctt
     3841 tgcgatgttt ttgccgatta gtttcttatt gtccatgttg ccgacttatg agagtttggg
     3901 caagaaggcg attattcgct tgttcaatac gattatgatg cgagcaggag ttactttggt
     3961 gattacgact gcatttagca tctccacgat gtttttcaat atttcggcaa cctatccatt
     4021 tttcatggtt gcttttctgc aaattgtgac ctttgccggt atctatttca aattgggcga
     4081 cattatgaat atgttcaatc tccagagtaa tgatagccaa tcaatgggaa gacgggtcat
     4141 gcgaaaaccg caaatgttaa tgaatcgaac attgcggcaa ctcaatcgaa atgtgggacg
     4201 gactttggct tttggtggtg gtgcagcagt tggaaataag ttggcaaagg aacaatcaaa
     4261 atccaaattt aagccatccg gttcttctct acgaaagaat tcacgattac cgaacgatca
     4321 cgagatacca tctgacttga ataaggaaaa gccaattcca aataataaga aacaatcccg
     4381 aatgaatttg gccgggagaa aagtcggcaa ggttctggat acccaagctt tggtgaaaga
     4441 taagaccaaa caagtaaagg atggagtacg gaatacgcca accaacttga aatacaactt
     4501 ccataaagga attgaaaaaa cgaaaaaagc accggaagag tttaagcgag gactggtcca
     4561 agaaaaagca aatcgaggag agttgcgaga aaaacaacaa caacgtagag acgaaaaaat
     4621 agctgaaaag cgcaaagtat tgaacgaaat agggaatcct catgggaaaa gaagggaaaa
     4681 tgttccgata aaagcaaagg tccaatctca aaaggataga gcaccaaagc ggattgtgag
     4741 agcaaattcg aatcccgaaa tcaaacgaaa attcgctagc caagaaatca tttcaaaagg
     4801 aataaaccaa tcctcaagta agaaaaattc ttttcaacaa gtgaaacggc ggtctactct
     4861 gtccaaacga accaatcgaa aagtacaaaa aagtttgaac cctcatccca aaccaaggtc
     4921 aggtgagaaa aaatgaacgt aaagaaatta agtgccattt cgttaacttt cctaacaatc
     4981 tcttttatgg gtatcttgat gtgtgtgggc ttattgtttg gtgaagacag tgaaggaagt
     5041 agtagttcag gtgctcctcc tggggggagt agtgtctcag aagaggtttt gaaacaccgt
     5101 gcaatggtgg aaaaatatgc caaagaatcg gatataacag agtatgttcc gattctttta
     5161 gcgattattc aagtggaatc tggtggcaca atggaagatg tgatgcaaag ttctgagtca
     5221 gccggtttac caccgaattc attaagtacc gaggcatcca tcaaacaagg atgtctctat
     5281 tttgcgtctt tggttaagcg ttccaaagaa ttaggttgtg atcaagatag tatcattcag
     5341 gcctataact ttggcggtgg ttatttggac tatgtggcaa aaaacgggaa gaagcatagt
     5401 tttgctttag ctgaatcttt ttcaaaggaa aaatcttgcg gtcaaaaaat cgattaccct
     5461 aatcccatcg cgaataagga aaatggtgga tggcgctata actatggcaa tatgttttat
     5521 tgcttattgg tgaagcaata cctaactaca acacagtttg acgataaaac agtccaaggg
     5581 atttttgacg aggcgtttaa gtatgaagga acggcttatg tatttggtgg gtcaagaccc
     5641 gaaacaggat ttgattgtag tggcttgact caatggtgtt atgccaaagt tggtctgaaa
     5701 cttcctcggg tggctcaaga tcagtatgat gccatgaccc atattgacct aaaagatgcc
     5761 aaaccagggg atttggtttt ctttcattcg acttatgatg ccggtactta tgtgacccat
     5821 gtcggtattt atgcagggga gaatcgcatg tttcatgcag gagatccagt aggctggacg
     5881 aatctaacag aaagctattg gcagcagcat cttatcggtg ctggacggta caaataagag
     5941 aaaagaggaa aaagaatgaa gataaaaatt gaacggaatc aaaaggaaaa gagcctaaaa
     6001 aagaaaaaag aacgagtcgt atccgtgggt aagcatcgaa aaatggtgct tgccctttgg
     6061 cttttattat cttgtagtct tacttttggc gtttataaaa atttcaccgc cattgatcaa
     6121 catacaactc atgaaaaagt ggttattaaa gagaaagtag tgaatacttc tggtattgaa
     6181 aactttacga aggatttttc gaaagaatat ttttcgtgga aaaataacaa agaagcaatt
     6241 gaaaagagaa tgaacaacct cgaacagtac cttacagaag aaggtttcgc tttaagtcaa
     6301 gatatggttc gagcagatat tcccactaat tccgaagttc agtcagtaaa agtcttagat
     6361 gttgaaaaga gctcggagga atttgtcgtt tcatttttag tggagcaaaa gattacagag
     6421 ggaaaaaagg cacaatcgat ttcttctgca taccgagtga cgatctttga agatgaaaac
     6481 aggaattata tagtaacgag tttgccaact atgattgcaa aacctgatag ggcaaaatac
     6541 aagacaaaac aagtagaaaa tgattcgaaa attgatgcca aaactacaga agaaatcgct
     6601 gagtttttgg aaacgttctt taaattatat ccatcagctt ctgaaaaaga attggagtat
     6661 tatgttgaaa atgatgcgat gcgaccaatc aatattgact taaagttttc gaagttagag
     6721 gatccagttt acctacaaaa cggaaaaaaa attctagtga gtacaaagat aaaatacctt
     6781 gataatgtat caaaaaccac tagctacttt cagtacaaat taatattaca aaaaaatgga
     6841 aactggaaaa tcattgatgt taaataataa tgactcaatc taaatttatg agaagttata
     6901 agtagtttaa cttatttcag gcagaagtcg cccatcctct ataggtgggt gatgaatgcc
     6961 gttcggtaac tcgaaaaccg acaattaaga catagtgcct ttttatttta atccatgtca
     7021 taatcagtct atataagaaa tggttgatat accatggaat tagatacaaa taatcattca
     7081 ttatttcttc tttgttacca ccttatttta gtagtgaaac attgtaaaca tctcattgat
     7141 gatgtaatgt tagcttatgc aaaaaactat ctttgaaaga atatcagaat cacatcacat
     7201 taccttagtt gagtgaaatc gtgataaaga ctactttcat attatgctca aagcacaacc
     7261 taaaaccgaa ttaacaaaat tcagcaatgc ctaagctcat gtctgataaa atgagacttt
     7321 ctaagggtca aaaatttcct ttgaatacga tcattagctt agataaaagt aaaaacctta
     7381 gaactacggg gatagcttgg ttaataagag atatcgctgt taataaagac ataagcttgc
     7441 aagtatgctc tattcccaag aatctcccac ttcaagcact aaggatcgtt aaattcagct
     7501 aagtggcaag ta
//
LOCUS       DANKHJ010000122         7487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000122 DANKHJ010000000
VERSION     DANKHJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..622
                     /gene="uxuA"
                     /locus_tag="PW113_002858"
     CDS             <1..622
                     /gene="uxuA"
                     /locus_tag="PW113_002858"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2527108.1"
                     /translation="LPGWEPERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEE
                     VGVKMAVHPDDPPYSIFGLARIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDV
                     YGILAEFCQRDRIPFAHVRNIKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGY
                     IRPDHGRMIWNETGRPGYGLYDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(730..2283)
                     /locus_tag="PW113_002859"
                     /pseudo
     CDS             complement(730..2283)
                     /locus_tag="PW113_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2431..3572
                     /locus_tag="PW113_002860"
                     /pseudo
     CDS             2431..3572
                     /locus_tag="PW113_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(3698..4777)
                     /locus_tag="PW113_002861"
     CDS             complement(3698..4777)
                     /locus_tag="PW113_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2527109.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4880..5320)
                     /locus_tag="PW113_002862"
     CDS             complement(4880..5320)
                     /locus_tag="PW113_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2527110.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(5377..5787)
                     /locus_tag="PW113_002863"
     CDS             complement(5377..5787)
                     /locus_tag="PW113_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2527111.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5967..6401)
                     /locus_tag="PW113_002864"
     CDS             complement(5967..6401)
                     /locus_tag="PW113_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2527112.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(6501..7349)
                     /locus_tag="PW113_002865"
     CDS             complement(6501..7349)
                     /locus_tag="PW113_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2527113.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2647 a         1353 c         1266 g         2221 t
ORIGIN      
        1 attgccggga tgggaacctg agcgcttagc tcctatcaaa tccttgcttg aagcctatgc
       61 atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat caatcatccc
      121 aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc catactcgat
      181 ttttggatta gctcgaatcg taaagaatcg agaagattta gattggttgt gtcgcatagt
      241 agacagccct tataacggaa tcactctttg tacagggagt attgcagaag atccagaaaa
      301 tgatgtatat gggattttag cagaattttg ccaaagagat cgaatcccat ttgcacatgt
      361 tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccacata aatccgaata
      421 cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat ttaacggata
      481 catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag gatacggctt
      541 atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg
      601 aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa ataagaagga
      661 tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc
      721 ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc
      781 ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt
      841 gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca
      901 gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt
      961 tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata
     1021 gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc
     1081 ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg
     1141 ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca atacgctatg
     1201 ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc
     1261 attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat
     1321 aaacaaatca aatacagtac aaagggactc caccatttct cttgaataaa gaacatgagg
     1381 aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt
     1441 cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag
     1501 attaggtcgc aaaatatcta cagagagatc aataaaaaag tcaatatatt catactctgt
     1561 gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa
     1621 tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa
     1681 aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt
     1741 acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc
     1801 agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact atgctaaaaa
     1861 tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact
     1921 gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac
     1981 tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat caattccttc
     2041 aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg
     2101 ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa
     2161 atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat
     2221 atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt cttgagtttg
     2281 catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc
     2341 ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg
     2401 catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca
     2461 tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga ggagacagaa
     2521 acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa
     2581 ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa
     2641 gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt tacatttgag
     2701 aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat ccaacacaag
     2761 caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt
     2821 actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta tagtcagcgg
     2881 cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga agaaccaaca
     2941 gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa
     3001 cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac
     3061 aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc agaaccagat
     3121 acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa
     3181 aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc agaaccagat
     3241 acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat tcaagaggat
     3301 ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga agagcagctg
     3361 aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa aagtagcaag
     3421 gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc ggagagtcac
     3481 aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca atgctttctg
     3541 caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt gaacgcattt
     3601 ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa atctgatatt
     3661 tggggtcgtt catactttat ctttcttcat atttatttta agggatatct gtttcttttg
     3721 gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta aagatcggaa
     3781 tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag accacaaatg
     3841 tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc acataagact
     3901 caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg atgcctcctt
     3961 gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct actgggatca
     4021 gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca aatacagcca
     4081 aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct tctaatacca
     4141 aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa
     4201 attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca atcataaaaa
     4261 agaaacttaa gatgaatgac atagcaaacg ccactgccat ttttcctata tcctgtaaat
     4321 agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat
     4381 aagtatggat atattgtaaa acaggatctg catcttttgg ctgatgctcg taaaaattaa
     4441 taacggactg gatcatgtca aatgtttgat gaataagcat cgggatatac tttgtgaatg
     4501 ccagatagat aaggtaaacg atcagtgtat aaatcatgat agctaaaaca cctgtaggga
     4561 ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat caaataagta aagataaatg
     4621 tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg acaaataata
     4681 aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga
     4741 caacctccaa ataacaaaaa ataaacacgt tattcattat atgatggaat atgaaaataa
     4801 gaaagagcaa aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa
     4861 aatcgttcat ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt
     4921 ttcgggtcag atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga
     4981 tcttttgtgg aataccttct gcttgttttt gcaacgcagc accttgagca gtcagcgtaa
     5041 catttacgat tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta
     5101 acttttttag taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt
     5161 tcactgaagt ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa
     5221 gattcagcgg cttgaggagg ggagtgtagt gtctaatgat ttctttagag gcggcatata
     5281 aaggaaaaca tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt
     5341 actatagtgt atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt
     5401 aacttctata tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa
     5461 taattcttgc gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa
     5521 gacaaagctc gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc
     5581 agagatattt ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc
     5641 agctgcaaac aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat
     5701 attcaattcg aatgtacgat cgggattatg cacttctcca gagcgtccgc ctgtgttgat
     5761 cattttcgta gaatacattt ttttcataaa gttaccacct ttaattatat tttatacaat
     5821 tgaattttac acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag
     5881 agaaagcaca tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaatatcaga
     5941 gacaaaaaat aatctcatca taaacattac atatatttct caaaaagttg ttttctttct
     6001 tcaaaaatta cttcacgatg gacctgatga agcatccata aataaccaaa aggatctctg
     6061 aacattgagt tagtcacacc aaagtccatc atttctgtca tttcttgaat ctctacacag
     6121 ccatttcgta atgctttatt atgtgtgtcc tcgatatcag agacgatgat attgaaccag
     6181 atcgtatttg gatgttcagc agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc
     6241 atatggaatc tcgtaccata caaggtaaaa atgacttcat tattgcctct agggtaatct
     6301 gttgcctcaa tcagttgtat ttcatcaaag attgtttgat acagttctag tgctttcaaa
     6361 ctatcattta atacaaaatc gatttccgtt cctttatgca taaagatctc ctctcctttt
     6421 cagtcgaaca gttactagat tcttttatta taccagctaa agaaaacgca ttcaaaaaaa
     6481 tagcggattt acttaccaac tcattcgatt ggatgataag taaatccgcg tcccattact
     6541 tctgctacgc tgctgacaat gacaaaggca cttggatcga tttcttctat caatggtttc
     6601 attcgggaaa attcatgatc agtcacaacg atcaataaaa cctcttttga ctttcgatta
     6661 tacccgcctt ttgcctctag cagggtaagg ccacgtccaa tctcagcact taagcgaagg
     6721 cggatgtctt ccaaataatg ttcactcatg accataacag atttctttcg attgatacct
     6781 gtttcgatat aagtcatgac aattgaagtg attgcaatag ataaaatagc ataaagaaac
     6841 tcttctattc caaaaataaa cagattaaga gaaacaacga atgcgtctgt aaaaaataag
     6901 ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg
     6961 ccgctagaag agttgttttt atataaaatc gcaactccta aagcaaaaat cgcacttcca
     7021 aaaatgacag acaacaaccg atctgaagta atcattgttt ctggaactat tgcaatgatt
     7081 aaaggaaaaa ccaagcttcc gaaaagtact ttaaaaaagg gttttttacc taaaaataga
     7141 agagctagta ccaacataac gatattcaac actaaaataa ctatagctcg gtcaaagcct
     7201 aaagcagctt ccagtaatat tcctagaccg ctgacacctc cagcagcaat atgatgaggc
     7261 cctaaaaaca tattgatagc gactcctaat atgaagaggg caaaaataat cttaatgtat
     7321 gtccatataa attgtctcgt ttctttcatt tattcacttc cttaattcat taataatact
     7381 cttatcttat tataacttac ttttcacctt aagttaactt taattaataa aaaaattagg
     7441 ctagtctaaa ttattatgtt tcattcttct agaatatact tctacgc
//
LOCUS       DANKHJ010000123         7353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000123 DANKHJ010000000
VERSION     DANKHJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            86..802
                     /locus_tag="PW113_002866"
     CDS             86..802
                     /locus_tag="PW113_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2527114.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            821..2404
                     /locus_tag="PW113_002867"
     CDS             821..2404
                     /locus_tag="PW113_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL2527115.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2539..3117
                     /locus_tag="PW113_002868"
     CDS             2539..3117
                     /locus_tag="PW113_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527116.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            3063..3581
                     /locus_tag="PW113_002869"
     CDS             3063..3581
                     /locus_tag="PW113_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527117.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3589..4629
                     /locus_tag="PW113_002870"
     CDS             3589..4629
                     /locus_tag="PW113_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2527118.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            4872..5810
                     /locus_tag="PW113_002871"
     CDS             4872..5810
                     /locus_tag="PW113_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2527119.1"
                     /translation="MQITYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVY
                     LFFKQRKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFP
                     ISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSN
                     EFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNL
                     FKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDF
                     NSVDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT         2708 a          882 c         1451 g         2312 t
ORIGIN      
        1 gaggaagaaa cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat
       61 ttttataaaa agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa
      121 aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa
      181 caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta
      241 tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa
      301 cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt
      361 agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc
      421 tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa
      481 aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca
      541 agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac
      601 acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt
      661 gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat
      721 tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca
      781 tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt
      841 agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac
      901 aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct
      961 cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc
     1021 acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga
     1081 atataatata gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc
     1141 gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg
     1201 ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc
     1261 agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg
     1321 aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa
     1381 agatatacag ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca
     1441 aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat
     1501 gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc
     1561 aggaaaaaaa tatcaattaa aaccattatt atatataatt gatgaaatac taaatagttt
     1621 ctatcagaag agttattggg gatattcatt accaaactat atttcagcag ttcataatgc
     1681 tcatcctaat tatgcaagct atctgtcata taagaatact ttaacaattg aagcaatgaa
     1741 tgaaatattc gaaatgatgg atgaagaaaa aaaggtatct tatgataaac aatatataga
     1801 agatttatac tacaaatata tggaaactgg gaaaatacag gaggaatata tggaagaact
     1861 gagaaaattt ctctcagaca aaaaaattct actgattgca ccaggaaaaa gttctgtaga
     1921 tgaggtagaa agaataaaag agtttgttac caaagaagaa gtcgtaataa ttagtgtaaa
     1981 ttttgaatat tctcatatag atatagatta cgtattttta agtaatttaa gacgttttca
     2041 tgaattaccg aaagaaagca gaactaaatg tattgttaca tcaaatatac aagcatcgga
     2101 tgtatattta cggacgagtt ataaaaagtt gttatctgaa agggaagttg taagagacaa
     2161 tgcaggatta atggcaatta gatttttagc cgatatgaat gtggatgaaa tattcttagc
     2221 tggatttgat ggatattcac ataatgaaga tgagaattat ggtgagcaat ccatggaaat
     2281 tataacaaaa tttgcattat tagatgcaat gaacgtgcaa atgagagatg aattgtcaga
     2341 attagctaaa aaagtaaagg tgacattttt aacggatcca cgttgggttc gaattgataa
     2401 gtagtagtaa taatgttttt aaaattttga taaagagata gtgagagaat aaaaatgggt
     2461 aaagtcttaa ataaaaaaga agaaaaatat ttaagggccg atggggcgag atgaaactct
     2521 aattttgaac ttttaagaat catttctata ttgtgtattg ttgcttacca tgctaatttg
     2581 cgtttaaatt ataatgcaga cggaattata gtacatggtt cattaactgc taaccagatt
     2641 ttcttgaatt atattggtat ttggggtcag ctaggagtta attgttttat atttatttcg
     2701 gcatattttt taagtgagaa aagaaacatt cactttgaaa atgttttggg aattgtatta
     2761 gaatgtattt tttatagtgt attagtttat ggaattcttt gtgcatgtgg aatagaaaca
     2821 ttttcattga acagctttgt aaaagaaata ttatcatttg ctctaggtag atattggttt
     2881 attacatcct atttagcatt gtatattcta agtccgtatt taaatatcat gcttaacaat
     2941 ctggaaaaac ttcagttgca aaaaataagt atgttattgt ttataggtgt atgctgttat
     3001 cgtatgttat ttaaaacatc tttgtttggg gattgtgctg ttttaattgc actatatgtc
     3061 atatgttata cactgaaaaa aacgataata atatttttga gagacacaga atcataggaa
     3121 caattgtatg tataacagtt ataccacttc tcatatccat aggaatactt ttcaaattta
     3181 taccaaatgc aatggttaac cagatggttc atattggttc tccattgatg gttatagcaa
     3241 gtatattttt attttatgta tttaaaaatt tttcatttta cagtaagtat ataaataaac
     3301 ttgcgagaac cagtttaggg gtatatattt tacatgaaaa tcattatttc attgatggta
     3361 tgaagtctag aggcttaata tgggatggga tatttggatt aggcaaaagg tttttagata
     3421 gggatttttg gatttggttt ataattgcag ttgtagttgt gtgcataatt tgcatggcta
     3481 tcgatgttgt aagagaaaaa tttgaaagac agatttttaa aattaagagt gtaaagaacc
     3541 tgtgtaaact tatgaatcat gttataaata tatacccata attaataaat gaggagaaaa
     3601 gaagtgaaag ttatatcatt cggagaatta ttattgagac ttgcagcacc cggttattca
     3661 aagttatttc aaagagatag tttggagact acattctgcg gaggggaagc taatgtagcg
     3721 gtatccttag caatatttgg atatcaatca gaatttataa caaagcttcc ggattcagat
     3781 gttggatatg cggcagctaa tgctttgcgt tattttggag taaatgttga taatattata
     3841 tttggagagg gacgcatggg actgtattat ttagaaaaag gagcagctca gagaccatct
     3901 aaagtaattt atgacagaaa attttctgca atttcgttag ctgatagtga tgaatttaag
     3961 tggaaggaga tcctaaacga tgcaaattgg tttcattgga cagggataaa tccagcattg
     4021 tctgagaatc tctttgagac gtccttagat gcatgtcagg ctgccaaaaa aagaggtata
     4081 acaatttctt gtgatttaaa ttatcgatcc aagctttgga catcagagga agcaaaagaa
     4141 aagatggaac agatactacc attggttgat atttgtattt gtaatgaaga agatgcagaa
     4201 aaagtgctag gaattaaagc tgagcataca aatattaata gtggagaatt aaataaagat
     4261 ggctatgtgc aggtggctag ggagattcac aagaagtttg gatgtaggta tgtagctaca
     4321 actctgcgta gaagttattc ggcaagtaga aatggttgga gtgcaatgtt atacgaagca
     4381 gaaaaagata aagcgtattt ttccacagaa tacaacatac aaattgttga tcgcgttggt
     4441 ggtggagaca gcttttctgc tggtataata tatgcgttat gtgaaaatat gtccggacaa
     4501 aaggctattg aatttgcgac tgcatctagt tgtttaaaac atactataga aggtgatttt
     4561 aatcgaacta caattgagga tgtatataat ttaatgaata gcggtggaaa tggtcgcgtg
     4621 ttgcgttagt caacatataa agataaatca cagtaatttt tatggtgatt atctgtctta
     4681 gtagtgatta gaataacgtg aaaataaatt gttgagcgtt tatactgttt tgaaaaataa
     4741 atttaacata ttgaaaaata caaatatata cgtatattat aaagcgccga ttgtaaaatt
     4801 aagctagaca actaaaacag tcatttgtgc tacactcaaa atagttccga ccaaagaatt
     4861 ataaggagtg agtgcaaata acctatacct atcttacaac ggatgaactt gtaataatag
     4921 agtcttattt gaaaattaac caatctgttg ctaaaactgc gcattgcttg aatcgttcaa
     4981 gtcaaacgat ccataaagta tatctgttct tcaagcaaag aaaatcagcc ttagaatact
     5041 atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt ttacccgagg
     5101 aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat gtgattgttg
     5161 gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg
     5221 ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat ggacatcaag
     5281 aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag gattatagcc
     5341 aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg aaacataaaa
     5401 gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg aaaccgtgtg
     5461 gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa
     5521 acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg aaacagatca
     5581 gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct caaagaggcc
     5641 taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct atggatttca
     5701 attcagtaga tgaatttttt attcaatctg tcgcatctaa acgaaataat attcctagaa
     5761 aatcactgga ttatcgaacg attatcgaac acctttggaa gtatttttga gttacgtaag
     5821 tattgatgat ctgtctaatt taatttgaca attaagaaag tattaacttg ttttgtgaaa
     5881 aagagaattt gaaaaacgat tggcgaacaa atgctcggta ggtatggtat aaatacagag
     5941 ctaattggtg tgatgaaatc agatattcgc gtgagattca gtaaagtagg agttcgaaaa
     6001 cagctaggtg acaaacagta ttgctttggt acttcggaat ggtttatcaa attcttatca
     6061 acagttcact tctctttatg tttgagtgat gattcgttag gttattgctt ggaaagaccg
     6121 gatgagagta gaagagttta attttataaa gtgaaaagag gtaatgtacg atatacgtag
     6181 ggctgaacca atagaggata agcaaacgat gttacaggcg caaaattcat agcttactgt
     6241 tttggagata acagaagagg aaataatgca gaagtaaggc gaaaaaagga cggaagccta
     6301 acagcatatg aaataaagaa aaatattata actactgata aatcggtatt gagcaaagta
     6361 ctaataggag tcaaaaacat tcataagcta tgagtgtttc ttggctactt ttttattttt
     6421 atgtatctac aaaagtttcc aggggcagaa cctgtgatta ttaatggaag aacctcaagt
     6481 attgtcttaa atcattgtta tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca
     6541 agttactttt cattacatcg aatttttcag aagttgctta tattggtgat gatattaatg
     6601 atttatcatg tatgacatct gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc
     6661 cacaagaaat gagattgaat tatctgattt tattgtaact cacagtggtg gtgacgaatc
     6721 tgtgatagag tttattgagt ggatattgga atcgatatag tgtagtagta cagaaaaatt
     6781 atcatacaat taatactagg cataagaata ttggcatatt ctagagttaa aagtaactat
     6841 tcgaaaagga taaggttaga attttttgtt ttgccagtgg aagaatataa aaagggcatt
     6901 ggaaattaat atattagaat gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt
     6961 gatgggatgg aaaaatacac ttatttcaaa caacgataat ataattatgt gaaccgaagt
     7021 atactcgctt ctaattcaat cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag
     7081 ttttaatgta tccccatatg atccgaaaaa tggtaattta caattaacag tttgatgatg
     7141 ttgaagatgt ggaaatgctg aaattaccat gatgagaaac acattaatta aagggaatta
     7201 attaactaga atataatctg attcaatttg aattaagcaa aaaaaggctg agttttaaat
     7261 aatcgcgaat tgtaaaatta agttagaaaa ataaaaaggc atttatggta cactcaaatt
     7321 gtatttccga cgaaagaaaa caaaggagtg taa
//
LOCUS       DANKHJ010000124         7307 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000124 DANKHJ010000000
VERSION     DANKHJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(406..780)
                     /locus_tag="PW113_002872"
     CDS             complement(406..780)
                     /locus_tag="PW113_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2527120.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="PW113_002873"
     CDS             complement(803..2203)
                     /locus_tag="PW113_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2527121.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="PW113_002874"
     CDS             complement(2207..2617)
                     /locus_tag="PW113_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2527122.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="PW113_002875"
     CDS             complement(2635..3126)
                     /locus_tag="PW113_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2527123.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="PW113_002876"
     CDS             complement(3136..3927)
                     /locus_tag="PW113_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2527124.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="PW113_002877"
     CDS             complement(3920..4693)
                     /locus_tag="PW113_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2527125.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>7307)
                     /locus_tag="PW113_002878"
     CDS             complement(4849..>7307)
                     /locus_tag="PW113_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2527126.1"
                     /translation="IAMKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFKYNQQKPK
                     DCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIYQYALDRK
                     VIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANGGYLFLDE
                     VHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLATFRRRIP
                     AEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIELFTDLLSKTFEGNVGELR
                     NEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEISIDLKKMFLKSLSEIDLIELFQ
                     NHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNYVVNKISPIIDSTGLCLLD
                     EFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEESKQEL
                     LLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGET
                     SDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIEC
                     GIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRY
                     LDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQT
                     LEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDV
                     KIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYC
                     IEIPKGEYELIYTIFEQTIF"
BASE COUNT         2357 a         1433 c          946 g         2571 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca
     5341 aattgtattc ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa
     5401 atcaaaaatc aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt
     5461 ttcagaaatc ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc
     5521 tttataatac tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat
     5581 tggctttttt tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt
     5641 taaaattacc aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga
     5701 tcccatatca actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt
     5761 gattaggtta attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac
     5821 atatgtattc agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg
     5881 cataactatc agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag
     5941 tatccatttc aacaaaagtt cttgtttact ttcttcgact tctaaactac tccaaatctc
     6001 atttgccaat aatttttgtt tatcattttt catacgaaat tttctgaact tagacagcaa
     6061 aaaatttctt gatgtttctt caattacatc aataaacatt ataaatagac tgatatccgc
     6121 caagaattca tctaataaac atagacctgt cgaatcaata attggtgata ttttattaac
     6181 aacatagttt cttaatgcta taaattcgtc actttctaaa taattatact cagaagaacc
     6241 tgcacactct ttaagtattc tatctcttaa ttgactcatg ctttcttcaa ttgaaatgtg
     6301 attttgaaaa agttcaatca aatcaatttc agaaagtgat ttaagaaaca ttttttttaa
     6361 atctatacta atttctggat ctatccaaaa accgctgtct agtttgtccc ctaaataaat
     6421 tgaagacttt ctcttatttc ctaaataacc ttctgcacaa agaagcttga tttcattcct
     6481 caattctcca acatttcctt caaatgtttt cgataaaaga tcagtaaaaa gttcaatcga
     6541 acaaaatatt tttgaatcaa tcgtaattgc ttcatcttta agaaattcaa ataataattg
     6601 aattttttca gatgtacttc tattattgta atctggcaga gtgatttcag ctgggatgcg
     6661 tcttctaaaa gttgctaaca aagaggtatt tttatcttct gttgtggcta agataaggcg
     6721 aacatttgca tactcccatt gcacactttc accaagttta cgatactttt gagaatccat
     6781 aagaagaaac aatttctctt ggttttctgc acttagattg tgaacctcat ctaaaaaaag
     6841 gtatcctcca ttagcagaag atagcatccc tgcagtatct ttttcagccc cagtaaaggc
     6901 tcctttttta tatccaaata aaactgctga caatagctct ggattatttg cataatctgc
     6961 acaattcaca acaacaaatt ttgcgtcatt atgaattact tttctatcaa gagcatactg
     7021 atagatcaaa cttgctaaaa aactttttcc aacgccactg tttcctttta ttattaatgg
     7081 gaggccatta gggggataca ttacagtagc tttgcattta tctatagtgg atttcaaaga
     7141 tatgtctgct ccaataaact tagaaaagca gtcttttggc ttttgttgat tatacttgaa
     7201 cattactggt ctaacagaat cattctttag taattttcca tctgaaaata actgattcaa
     7261 atagtgggaa gcgacatttc ttttaataca aaatttcatt gcaatat
//
LOCUS       DANKHJ010000125         7256 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000125 DANKHJ010000000
VERSION     DANKHJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1533
                     /locus_tag="PW113_002879"
     CDS             <1..1533
                     /locus_tag="PW113_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HDL2527127.1"
                     /translation="PEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVTIALKH
                     FQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPARANEEK
                     WQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKKKGQKK
                     WWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVATKTEE
                     IADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAEASLKE
                     LGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVTLSSHV
                     GENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYINFVVS
                     QGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGAGTTIT
                     KGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSASTSES
                     SK"
     gene            1698..2597
                     /gene="rsgA"
                     /locus_tag="PW113_002880"
     CDS             1698..2597
                     /gene="rsgA"
                     /locus_tag="PW113_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2527128.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            2610..3260
                     /locus_tag="PW113_002881"
     CDS             2610..3260
                     /locus_tag="PW113_002881"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2527129.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            3261..3899
                     /locus_tag="PW113_002882"
     CDS             3261..3899
                     /locus_tag="PW113_002882"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2527130.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            4054..4908
                     /gene="mreC"
                     /locus_tag="PW113_002883"
     CDS             4054..4908
                     /gene="mreC"
                     /locus_tag="PW113_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2527131.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            4912..5421
                     /gene="mreD"
                     /locus_tag="PW113_002884"
     CDS             4912..5421
                     /gene="mreD"
                     /locus_tag="PW113_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2527132.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            5676..7250
                     /locus_tag="PW113_002885"
     CDS             5676..7250
                     /locus_tag="PW113_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2527133.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         2458 a         1383 c         1530 g         1885 t
ORIGIN      
        1 cctgagcagg ccagaggaag tatggcaacg aagcagtcag atatttatgc tttagggatc
       61 atcctttatg agatgttgac gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt
      121 gctttaaaac attttcaaga tgatcttcca tctataaaag cacttgatcc aaatgtgcca
      181 caagcactgg aaaacgtgat tttacgggca acggctaaag aacctgcaga ccgttataag
      241 tcagccgagg aaatgtctga tgatctttct acagtattat caccagctcg tgcaaacgaa
      301 gaaaaatggc agcctcatgt gatggataat gaaacaaaag ttatcacacc ccttacggaa
      361 gatgcaccaa tgcctgattc attcaaatct atgcctctgc caaaagacaa gccagaaaac
      421 gaaaccttac cagaggagga agaagttctg cctgatccta aaaagaaagg acagaaaaaa
      481 tggtggctga tcctattagc tattcttgct gttttaggaa tcggaacagc tgtattcttt
      541 gcttctggcg ggcgaggaga agttgttgtt cctgatgttt ctgatctttc tgaagcttct
      601 gctagagaaa cactcgcaaa agcgaaatta gaagtagcaa caaaaacaga agaaattgca
      661 gatgacaaaa tcgaagaagg taatgtagtg aagacagacc cggcagcggg aacgacagtc
      721 aagcaaaaac gcgaagtcac tctgtatatt agtagcggca cgaaaaaaat caaattagat
      781 gattacagtg gtaaaagtta tgaagatgcc gaagcaagtt tgaaagaatt agggttttct
      841 gctgacttga ttcaaaaaga agaggaattc agttcagagg tagaaagcgg aatgatcatt
      901 tcgcaatctc catcagaaga tacagaagtc gatcctaaaa cagacacgat tactttcaca
      961 gtcagcaaag gaccagaaac agtgactttg agtagtcatg taggagaaaa ctacgaaact
     1021 gttcgtaatt ggcttgttac acaaggattc aatatcaata atattagtgc tacttatgac
     1081 tatagcgaca ctgtaccatc aggttcgatc atcagtcagg atccagcctc gggtgaagtt
     1141 gtagcagaag aaacttacat caattttgtc gtaagccaag gaacagagcc taaattgaat
     1201 gatatctccg gctatacaaa aagcgaagca caaagttatt tagctagtgt cggtgcagaa
     1261 tatatcgggc atgagacttc cgaatattca aacacggtgg ataaagataa agtgattcgt
     1321 acagtaccag gggcaggaac gacaattacc aaaggaatgg ttgtgaacgt aatttactct
     1381 aaaggaccag atccagcgtc ttctagttct gaaaaagact cttcagacag ttctagcagc
     1441 tcaagtagct ccagtagcac aagcaactct tcaaaagaag aatcttccag ctccacaagt
     1501 tcatcagcta gcacctcaga aagttcaaaa taagtgctga ataaaatata tcagaaggga
     1561 atgcgacatt gatttgttgc attctctttt ttgtgtttct atctcttttg atcaattttc
     1621 aagccatata ctttgagaag tttgcatgga ataacaccta ctttcctctt cgtatggtaa
     1681 aataaacaaa aacggaggtg gcacttatca aaggacaaat cagaaaagca atcagcggtt
     1741 tttactatgt atacgcaaac ggagagacct atcaaacaag aggaagagga aatttccgga
     1801 atcggaaaat cacgccgctt gtcggagatc aggtgatctt tgagagtgaa aatcaaacag
     1861 acggctatct tctggaaatc ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa
     1921 atgttgatct tggaatcatt gtcacaagta tcgtagagcc tgcattttct tataatctac
     1981 ttgaccgctt tctagttacc ttggaatacg agtcgatcga accgattatt tttttgacaa
     2041 agaccgactt gtcagacgac ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa
     2101 taggctatcc ggtgattgta cccaatcata cgggtgatac gacagaactg atccgttact
     2161 tcccagaacg gctatcggtc tttatgggtc agtctggagc aggaaaatcg acgctactga
     2221 atcaaatatc accggaactg aatttagcaa caggagagat ttctgatgca cttggaagag
     2281 gacggcatac aacacgtcat gtggaactga ttcctatcta tggcgggtta gtagcagata
     2341 caccaggatt tagctcgatt gactttttga caattgaagc aggagaactg ccgagacaat
     2401 ttcctgaatt tgtagaagct tctgtccatt gcaaatttag agagtgcatg catgctaaag
     2461 aacctggctg cgaagtaaaa catagagtag aaacaggtgg tatcgcacag acacgttacg
     2521 acaattattt gcaattctta caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag
     2581 aaggtaaacg aaaataggag gaaacaagaa tgaagatagc accatccatt ttgagtgcag
     2641 attttgccaa tttacaaaga gacattgagt tagtggaaaa aggaggcgca gattacatcc
     2701 acgtagatgt aatggatggc cgatttgtcc caaatatcac attagggcca aatattgttc
     2761 aggcaatccg tcctgttact aaattaccgt tagacgtcca tctgatgatc gttgatccag
     2821 aaaactacat tccagcattt gcaaaagctg gagcagatat cattactgtc cacgtcgaag
     2881 caactcctca tattcatcgg gcacttcaaa tgacaaaaag cttaggagtt aaagctggag
     2941 tagttatcaa tccaggaaca ccagtctcca tgatcaaaca tgttttgcct ttggctgatc
     3001 aagtactagt catgactgtc aatcctgggt tcggcggaca aagctttatt gaagaaacag
     3061 tagaaaaaat tacggaactc gctgatctac gtagagagaa caattggact tatgcaatcg
     3121 aagtagatgg cggaatcgtt ccagaaacag caaaaatctg taaagatgca ggggcggatg
     3181 tcttcgttgc aggatcatat atctatggag ccgaaaatcc agtcggacaa atcgaacgat
     3241 taaaagaggc attagactaa atgaaaattt tattagctgc tggaggtaat ccgcaaaact
     3301 ggccgcagtc tctgctgtcc aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt
     3361 acctgcttca agcaggctac ccagtagatc tggcagtggg tgattttgat tcattgtcga
     3421 acgaagagca gaaggaagta tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa
     3481 aagacgatac agatacacag ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg
     3541 aagtcacttt gattggagct acgggcggaa gattggatca tcttttagcc aatctctggt
     3601 taggtatgga acctcgcttc aaaccctttg cccatcagct tattatcaga gattgtcaaa
     3661 attatttgtc ttattatttg cctggagagc atatagtaag acagatacca gatatgaagt
     3721 atttagctta ttgctgcttg actcccgtct cgaatcttac attaaaaggc agcaagtatg
     3781 tattggaaaa taaagaagta gaatatccga catcttatgc aagtaacgaa tttctttcag
     3841 ataccgcaga attttctttt tcagatggta tgattgcagt tattcaaagc aaagattgat
     3901 ccataaacaa ataaaaaaat ttgtctttca ggctaaaaat gacagaatct attatttgct
     3961 aaaaaaaagc ttaagaggag caacttacct tttgtttcac ggcatttagt gataaaattg
     4021 aacacaagag tctaattaat tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat
     4081 attattatta ccttggttat tgttatcatc gtggtgacga tactaagtgt gaccgctgcg
     4141 cagcgagcga atgaaggaaa gcccaatttc ttccaatcca tcgttaatga cagtgttgca
     4201 atggttgata aaacaatctc tgccccagta cggtgggtac gaaatggagt agattctgtc
     4261 cataatcttt tttcaactta tgccgaaaat gagcggctaa aagaaaaaat cgatagctat
     4321 gatgacgttt cacagcaaaa caagaattac gagagagaaa tcgaagcgtt gaaaaaagaa
     4381 cttgagttga atcagacgct gactagttac gaaaaagtaa cggcaaatgt cattactcgt
     4441 tcgcctgaca gttggcagga catgctgatc gtagacaagg gttctaaaga cggtatcgaa
     4501 gcaaatatgg cagtcatgtc gcaaaaagga ttgattggcc gagtgatcga ggtcaatacg
     4561 gcttcgtcta aaatcgaatt actgtcatcc tctaatgaaa gctccaatca ttttccagta
     4621 cgggtatctt cggctaatgg cgaagcgttt ggtttgctta aaaactatga tgaaaagctc
     4681 catgccttag tggtgaccca attaactggt gatacggata tcaaagaagg ggatgttgtc
     4741 cagacatccg gtcttggagg gaattctcca gctaacttgc cgatcggtac ggttattaaa
     4801 acgaaaccag atagttatgg gctggatcgg gaagtttatg tgaaacctta tgcagaaatg
     4861 tatgacgtgt cagttgtgac gattgtccaa cggctagcgg aggattagtc catgttgaag
     4921 aaagaaacaa tgaagtacta tctgccaatc gttttgttct ttttgatgtt gatagatggt
     4981 catttaacaa gaatgctagg ggagtggtcg aaaggcacct atatgtcaaa tgcccacttt
     5041 ctgatattgg cattattatg ttgcagtatg gcgtttgaaa aacgttattt actgattacc
     5101 acgattgttc tcggggctat ctatgacgct tactatattg gcgttatcgg tatctatgca
     5161 gtagctctcc ctttaattgt atggttgatg tatgtaatga aagacgttat ccatgtcaac
     5221 atctttactg aatttttcag tatgatcatc tttgtcacgg gttatgaatt gtttacgatg
     5281 gtggtccagt tgatttttaa attagcagta gtaaataaca cgtattttat tacaaggttt
     5341 ttaggaccta cactgctgtt gaacatgatt atatttgtat tattcatttt tccctttaag
     5401 aaattattca gtgatgaata atttcttttt ttatttttag gtcgaaagcg aaaaagaatc
     5461 gttgttatct taagaaatgc tagacttaac gcggcttttt catgatcatt ttctgaaacg
     5521 aacagttttg tcatttttaa aagttgtttt gaaacatttt tgtaacataa ctattatatc
     5581 gttgacacac tgctatggta caatttttat atcgttaaga aagacttaaa tatatttaga
     5641 tatttattaa attaagccag tcggaggaat gaagagtgaa aaagagttta atatcagcag
     5701 taatggtatg ttcaatgacc ttgactgccg tagcatcacc gatcgctgcc gcggcggacg
     5761 attttgattc tcagatacaa cagcaagatc aaaagattgc agacttgaaa aatcaacaag
     5821 cagatgcaca atcacaaatc gatgccttag aaagccaagt ttctgaaatc aacacacaag
     5881 cacaagacct attagcaaaa caagatacat tgcgtcaaga atctgcacaa ttagtaaaag
     5941 acattgcaga tttacaagaa cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac
     6001 gcgaagcaca agtaagcaac acaagctcaa actacatcga tgctgtattg aatgctgatt
     6061 cattagcaga tgctattgga cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg
     6121 acttgatgga acaacaaaaa caagataaaa aagctgtaga agacaaaaaa gctgaaaacg
     6181 atgctaaact aaaagaatta gctgaaaacc aagctgcttt ggaatctcaa aaaggcgatc
     6241 ttttatctaa acaagctgat ttgaacgtac tgaaaacttc attagctgct gaacaagcaa
     6301 ctgctgaaga caagaaagct gacttgaacc gtcaaaaagc agaagctgaa gcagagcaag
     6361 ctcgtattcg tgaacaacaa cgtctagcag aacaagctcg tcaacaagct gcacaagaaa
     6421 aagctgaaaa agaagcacgc gaacaagcag aggctgaagc acaagctact caagcttcat
     6481 ctactgcaca aagctcagca acagaagaat catcagctac acaatcttca atgactgaag
     6541 aatcatcatc agctacacaa tcttcagcaa cagaagaatc aacaacacct gaatcttcaa
     6601 cagaagaatc aacagcgcct gaatcttcag caacagaaga atcaacaaca gcgcctgaat
     6661 cttcagcaac agaagaatca acaacagtgc ctgaatcttc agcaacagaa gaatcaacaa
     6721 cagtgcctga atcttcaaca acagaagaat caacaacacc tgcgcctaca acaccatcaa
     6781 cagatcaaag tgttgataca ggaaacggca caggaagtag tactccggct ccaacgccaa
     6841 caccaacacc tgaacaacca aaacctgtaa cacctgctcc agctccttct ggaagtgtga
     6901 atggtgcagc aatcgtagca gaagcttaca aatatattgg tactccatat gtttggggcg
     6961 gtaaagatcc aagtggcttt gactgctcag ggttcacacg ctatgtttac atgcaagtaa
     7021 ctggtcgtga catcggtggt tggacagttc ctcaagaatc agctggaaca aaaatttcag
     7081 tttctcaagc aaaagctgga gacttgttat tctgggggtc acaaggcgga acttaccacg
     7141 tagcgattgc tttaggcgga ggacaatata tccatgctcc tcaaccaggc gaaagcgtaa
     7201 aagttggttc agttcaatgg tttgcccctg actttgctgt cagcatgtaa gaatga
//
LOCUS       DANKHJ010000126         7141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000126 DANKHJ010000000
VERSION     DANKHJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..615
                     /locus_tag="PW113_002886"
     CDS             <1..615
                     /locus_tag="PW113_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2527134.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="PW113_002887"
     CDS             612..1385
                     /locus_tag="PW113_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2527135.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="PW113_002888"
     CDS             1382..1780
                     /locus_tag="PW113_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2527136.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="PW113_002889"
     CDS             1965..2636
                     /locus_tag="PW113_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527137.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="PW113_002890"
     CDS             2695..3135
                     /locus_tag="PW113_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527138.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="PW113_002891"
     CDS             3147..3815
                     /locus_tag="PW113_002891"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2527139.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="PW113_002892"
     CDS             3879..4502
                     /locus_tag="PW113_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2527140.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="PW113_002893"
     CDS             4558..4944
                     /locus_tag="PW113_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2527141.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="PW113_002894"
     CDS             5157..6548
                     /locus_tag="PW113_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2527142.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DANKHJ010000127         7097 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000127 DANKHJ010000000
VERSION     DANKHJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(15..1154)
                     /locus_tag="PW113_002895"
     CDS             complement(15..1154)
                     /locus_tag="PW113_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2527143.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="PW113_002896"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="PW113_002896"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2527144.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /locus_tag="PW113_002897"
     CDS             complement(2967..3722)
                     /locus_tag="PW113_002897"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2527145.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /locus_tag="PW113_002898"
     CDS             complement(3826..5016)
                     /locus_tag="PW113_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2527146.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="PW113_002899"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="PW113_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2527147.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..>7097)
                     /locus_tag="PW113_002900"
     CDS             complement(6168..>7097)
                     /locus_tag="PW113_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HDL2527148.1"
                     /translation="RRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEV
                     YQSLENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRL
                     LPEGENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMA
                     AKANGNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRK
                     MTEKELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSK
                     AKAIRAYMKNAPKQTWLITDEAAANEILKGVTL"
BASE COUNT         1822 a         1509 c         1333 g         2433 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacg
//
LOCUS       DANKHJ010000128         6916 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000128 DANKHJ010000000
VERSION     DANKHJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1064)
                     /gene="glmS"
                     /locus_tag="PW113_002901"
     CDS             complement(<1..1064)
                     /gene="glmS"
                     /locus_tag="PW113_002901"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2527149.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQV"
     gene            1474..1764
                     /locus_tag="PW113_002902"
     CDS             1474..1764
                     /locus_tag="PW113_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527150.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            1777..2802
                     /gene="sppA"
                     /locus_tag="PW113_002903"
     CDS             1777..2802
                     /gene="sppA"
                     /locus_tag="PW113_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2527151.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            2805..3464
                     /locus_tag="PW113_002904"
     CDS             2805..3464
                     /locus_tag="PW113_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2527152.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            3621..4553
                     /locus_tag="PW113_002905"
     CDS             3621..4553
                     /locus_tag="PW113_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527153.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            4707..5996
                     /locus_tag="PW113_002906"
     CDS             4707..5996
                     /locus_tag="PW113_002906"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2527154.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6243..>6916
                     /gene="clpX"
                     /locus_tag="PW113_002907"
     CDS             6243..>6916
                     /gene="clpX"
                     /locus_tag="PW113_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2527155.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQG"
BASE COUNT         2338 a         1168 c         1471 g         1939 t
ORIGIN      
        1 acttggcggc tatctgctgt ttcgccactt tgactcaaga aaataaagaa tggttttttt
       61 gaaagaagag gtgtgttata gccaaattca ctggctaaat gaacctcaac agggatctgt
      121 gtcaattttt ccagagtctg tttacctgct aaccccgcat ggtaactagt tccgcaagca
      181 acgatataga ttcgatcgct atcagacaat gtatcgacca gcttttgatc aagagagaca
      241 cgattgtctt ctgttacgta tttttgagcg atacgacgca tgataatagg ctgttcgtcg
      301 atttctttca gcatataaaa aggataggta cctttctcga tatctgaaga atctacttgt
      361 gcttcgaatg aagaacgttc tacgatgtca cctgcaaaat tttgaataat aactttttct
      421 gatgtgacgg taaccatctc ttcatcttca atctcaacga attccttcgt atgagcaagc
      481 atagccattg catcgctggc aatcacattg aatccatcgc cgagtccgat taatagtgga
      541 cttttatttt tagcgacgta aataacatca ggtgctgtcc gatcaatcaa agcgaaagcg
      601 taagaacctt tgatgacccg caaagctttt aaaaacgcct cttgcgttgt agtatccttc
      661 gcgaaagttt caattagatg agcgataatc tcagtatcgg tctcaccaat aaagtggtca
      721 tttgctaaat aagcaatttt aagttcttca aagttttcga ttacgccatt gtgaacgagc
      781 acgaagcgtc cactttggga agtatgtgga tgggcgtttt cttcagaagg ttgcccgtga
      841 gttgcccagc gtgtatgacc aatacctata tttcctgtag tatcaatatt tattttgttt
      901 ttcaaatttt gaattctgcc ttgtgctttg accagataat cagttgtgcc atctgagaca
      961 aagataccag ctgaatcata accacggtat tctaattttt ctagaccgtc aatcaatcct
     1021 ggtgttacat tttttaatcc aatcattcca acaattccgc acataaaaaa catcctactt
     1081 tctataatga attcgtatga atgcttccag ctgcaggaaa atccttttgt tttatctttg
     1141 cagatatttt ttgtggattt tctgtagagt gcagtcccta gggccatccg ccgaatcttt
     1201 cgataaaccc tctcctcgtc atctgtttac acagacctgg cgctatccta aaaggtataa
     1261 ctatcctcct cttttgtttt ttatctggaa acatttaaaa tcataacctt ttgcgaatgt
     1321 tcttgtcaac aaaaaaagaa taaaaaataa attggtatat atcaaatgct ttgtcaaatg
     1381 ctctttttat gtcaaaaaat aatgggtttt tatctatcat ctttttcagg agttcggtac
     1441 aatagaaagt aatcggatag aggaggagta aaaatggatt caaaaattga gatcatgaca
     1501 ctgggaatgt taaaaaaaca attgtccaaa ttcgaagcat cagctggtgt atcagatgat
     1561 acgaaaatat ttttagatac tggctgggat agcatccaag aaatcgcacc tgatgccttg
     1621 gaagttgctg aagctcgtga gtttacggta gaagatgaat tgacaaaaga gtcgttttcc
     1681 gggtatgcgc gggaagaaaa agcagagcgg ttcgacactt ctgagcagtc agaaactgtg
     1741 atcgtcataa aaaacttgta ttagaaagga gaaagaatga ataagagacg ttgggcagca
     1801 gttctgattg ctgcaggatt atttgtggtt tcgttagttt cagctagttt gactaagcct
     1861 caggaaaacg atcaaattga gggattgaac agttggttat atggggatga agaactagct
     1921 tcaaatgtgt tggaagaggg agacagtacg caaaaaatcg taaaattatc agtagatgga
     1981 acgattgctg cgggagggtc ttcaggttta tttgcttctg aaggttacaa tcataaaaat
     2041 tttatggaac aactgaaggc gattgaagaa gatccaacag tcaaaggtat ctttttagaa
     2101 gtaaattctc ctggtggcgg tgtctacgaa agtgcggaaa tcgctaagaa attggatacg
     2161 attcgtaaag aacatgatat cccaatgtat gtcagcatga aaaacatggc agctagcggt
     2221 ggctactata tttctgctca agcagataaa atctttgcaa cagaagaaac agtgaccggc
     2281 tcaattggtg tgatcatgtc aggattgaat tattcaggac ttctagaaaa actgggtgtg
     2341 gaagatacaa cagttaaaag cggagcgttg aaagatatgg gttcaggaac gcgaccagag
     2401 acaaaagaag aagaagcggt gcttcaagca tatatcgaca gcgcatatag tcgttttgta
     2461 gcaatcgtca gcgagggacg gaataaatct gaagaagaag tcaaaaaaat tgctgatgga
     2521 aggatttatg atggtgtcca agcaaaagaa gtaggtttag tcgatgaatt gggatttcct
     2581 gaagatgctt taaaagctat gcgaaaggaa caacagcttg aagatgcgga actagtagaa
     2641 tattcttcca cgccatctgg gtttgctagt acatggttag gaagtaaact agcagaattc
     2701 cagggattga agccttctga gactagtcag atcttatccg ttcttgagaa tctagggaca
     2761 gcggaatcac caaaagcaat gtattattac ggaggtgagt aagcatgtca gagctttcaa
     2821 atgaaaataa ggaacaagaa aaagataagc aacagcagcc tgctgtagga cctgactcca
     2881 acttttccca aaaggtactg agctcttttg aaagagagcc tttgacgccc gaagaaatca
     2941 aaaagaaaca acgtaaatgg aaaaagtata cggacacaag aaatgaaccg ccaaaagcaa
     3001 atgattttcc taagtatttc tatgcaggtt tttttatccg aatgtttgct ttcttggtcg
     3061 atcttttgtg tatcggagct attacgcgtt taacacttgg tattgccaaa aatctaggtt
     3121 ggcttaattt ttcagattct tatctgagta tctacggatt cacagcctta cttatttatc
     3181 tagcgtattt tattttattg acgaaattca atcatggtca aacgattggg aaaatgatct
     3241 ttggtattcg agtcatcagc tttgatgaag aagaacttag ctggtcaacc gttttagtta
     3301 gagaaggtgc ttgccggttc attctaaaat ttcctttgct gatgttaggg tatcttccag
     3361 caatctttaa tcagaaaaag caacatctag gcgatttttt cactaataca agtgttgtta
     3421 cgatcaatct gatcaaagca ttcaaccagg aagtcaacag ctgatgagca ataaaaagca
     3481 gtaacagttg aaagcacctt ctaaaagtca gatctaaaat ctagctttta gagggtgctt
     3541 cagttatttt taattgtcat gctttttttg ttttgctact attgaacatg agctgaacat
     3601 gagcaagagg aggacactgc gtgaagccga ttgattttcc agaaaaatat gagaatttaa
     3661 tgcgagtagc acaacaagca ctagcaaacc agcaatatca gcaggcaaaa gaattatttc
     3721 aacgagctta cgagttgaaa gaaagctttg aggctaatag cctgcttgtt ttttgtcttt
     3781 acgagttgga cgagaaaaaa gaagcattga agcaagcact cttgcatgaa aaacagtatc
     3841 ttcaaaatga ggagtttgct gaattttatt ttgacttgtt aatcagtaca caagattttt
     3901 tatatgctag aaaattgata gcaagtacag atttttatga agctttcgag cagcgggtca
     3961 ttgaaaaaat ccaatttgca gaagaattat ccggacaaat ggagaggcag aagatcaagg
     4021 cactccacaa taaatcggag gaacttcctt cgttggaacc tgcacgtcag cttgcgtcta
     4081 ttgatgagat cgaacagctt ccttatcatg agtttatcca aacagcgagt aaacttattg
     4141 tatcgccaga ggtccatatt ttagcaaggg caaaattact ggagacactt aggcaattga
     4201 acgaatgcca tcctgttttc tatttgacga ttgaagaaaa gttagtcaaa gtcataccaa
     4261 aagatctgcc aaagccgcag cagcaggcaa gttaccagca attatgtgta ttttcagatc
     4321 gttacggtaa tgaagatgcg ctgcttagtt ccgtgttaaa tgaagaattc acgttgcaat
     4381 ctgcaattgt ttatcctgtc tatgatacat acatcgaaga cccgaagcgc tggtttcaac
     4441 tgactgtaga ggcgtgtaca ggaaaaacga tgtatgaagg tacagaagat gaaaaacaag
     4501 attttttaaa aaaaagagaa aaaattttgc agcaaatgat cttcttccat tagattttat
     4561 cagtgaaaaa gataaaaatg ttatgaaaaa caaggattct atccattcaa tatgtttaca
     4621 atttgaaagg gttagtgtac tatttaaagg tatgcatgaa acgtttttga tgtataaaat
     4681 gaatttctaa actcggaggg aattaaatga ctgcaacatt tgaaaaaaaa ggcacgaatg
     4741 atggtgtttt aaaatttacg attgcgcaag acgaaatcca aaaaggatta acaactgcgt
     4801 ttaataaagt gaaaaaaagc ttgaacgtac ctggattccg taaagggaaa gtttctcgtc
     4861 aagtattcaa ccgtatgtac ggtgaagaag ctttatatga agatgcattg aatgctgtat
     4921 taccagaagc ttatgaagct gctgtcaaag aagctggcat cgatcctgtt tctcaaccaa
     4981 aaatcgatgt tgaaagcatg gaaaaaggtc aagactgggt aatcactgct gaagtaacag
     5041 tgaaaccaga agtaaaatta ggacaatata aagatctagt tgttgaaaaa caagaccgtg
     5101 aagtaacaga tgctgacgtg gatgcacgta tcaaacgtga acaagaagca caagctgaat
     5161 tagttatcaa agaagatgct gcagctgaaa atggcgatac agtagtaatc gacttcgaag
     5221 gctttgtaga tggcgaagcg tttgatggcg gaaaaggcga aaattactca cttgaattag
     5281 gttcaggctc attcattcca ggttttgaag atcaattagt tggtcataaa tcaggtgaaa
     5341 gtctagatgt aaatgtaact ttcccagaag attatcaagc agaagatctt gctggaaaag
     5401 aagcagtctt caaagtgaca atccatgaag taaaagcaaa agaagtgcct gaattagatg
     5461 acgaattcgc aaaagatgtt gatgacgaag tatcaacatt agacgaattg aaagaaaaat
     5521 atcgcaaaga attagtagaa acaaaagaaa aagcagcgga cgatgcaaaa gatgaagcag
     5581 cgatccgtat ggctgttgaa aacgcagaaa tcgttgaatt gccacacgtg atggttcatg
     5641 atgaagtaca tcgttcaatg gacgaattct tgaacaacat gcaacgtcaa ggtatctctc
     5701 ctgaaatgta ctatcaatta actggttcta cagaagaaga cttgcataaa caattcgaag
     5761 gtgaagcaga aatgcgcaca agaacaaatc ttgtgatcga agcaattgca gctgctgaaa
     5821 atcttcaagc aacagaagaa gaaatcaatg cagaaatcca agaattatca gaaacataca
     5881 acatgccaat cgaacaaatc aaacgcgtgt tgacagaaga tatgctggaa catgatgtga
     5941 caatgaaaaa agcaatcgaa ttgatcacag gtacagcgac agaagcgaaa gaatagaaat
     6001 caagtaataa atcatttctg aagaagctac ttctagaaaa ttgtttattc gaaataaggg
     6061 actgtgacac gattttgtca cagttcctta ttttttgtta agagtaaaaa atgcaaaggt
     6121 ataggtcagt tgatcctttg ttaaacactt tttcttttta ctaaactatg ttatgatagt
     6181 aaaagactgt aaagtctttg atagtttctt gcatactttt tgcaagttaa ggggtgaaga
     6241 ccatgtatga caatccaagt agcaatgaga ccgtacgctg ttctttttgc gggaaaaccc
     6301 aagaagaagt gaaaaagatc gtagctggtc caggggtcta catctgtaat gaatgtatcg
     6361 acctatgtaa agaaatcatt gatgaagaat tttatgaaga agctgtacgc gaattcactg
     6421 aagtccctaa accactagaa attcttgaag tattgaataa ctacgtgatt ggacaagatc
     6481 gcgcaaaacg ctcgttagct gttgccgtat acaatcacta taaacgtgtc aatcagcaag
     6541 cacaagaaaa cacagatgat gttgaactgc aaaaaagcaa tatctgcttg atcggaccta
     6601 cagggtctgg taaaacattt ttagcacaaa cattagcaaa aacattgaat gtaccttttg
     6661 caatcgcaga tgcgacaagt ttgacagaag ctggttatgt gggggaagat gtagaaaaca
     6721 ttttgcttaa attgcttcaa tctgctgatt acaatgtgga acgtgcagaa aaagggatca
     6781 tctatattga tgagatcgat aaaattgcgc gtaaaagcga aaatgtatct attactcgtg
     6841 acgtttccgg cgaaggtgtc caacaagcct tgctgaaaat tctagaagga actgttgcaa
     6901 gcgtgccacc tcaggg
//
LOCUS       DANKHJ010000129         6899 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000129 DANKHJ010000000
VERSION     DANKHJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..444
                     /locus_tag="PW113_002908"
     CDS             <1..444
                     /locus_tag="PW113_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2527156.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="PW113_002909"
     CDS             444..1643
                     /locus_tag="PW113_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2527157.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="PW113_002910"
     CDS             1659..2303
                     /locus_tag="PW113_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527158.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="PW113_002911"
     CDS             2314..3120
                     /locus_tag="PW113_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527159.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="PW113_002912"
     CDS             3142..3372
                     /locus_tag="PW113_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527160.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3886..4194
                     /locus_tag="PW113_002913"
     CDS             3886..4194
                     /locus_tag="PW113_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527161.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4196..4618
                     /locus_tag="PW113_002914"
     CDS             4196..4618
                     /locus_tag="PW113_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527162.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4638..6227
                     /locus_tag="PW113_002915"
     CDS             4638..6227
                     /locus_tag="PW113_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527163.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6239..6574
                     /locus_tag="PW113_002916"
     CDS             6239..6574
                     /locus_tag="PW113_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527164.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6715..>6899
                     /locus_tag="PW113_002917"
     CDS             6715..>6899
                     /locus_tag="PW113_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527165.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2647 a         1036 c         1419 g         1797 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca aaatatttaa
     3781 aaataggata gtgaaaggat agcaaaggat agtaaaagga tagaagcagg atagcgaaaa
     3841 ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata
     3901 agaaatgtag atgagaaaat cgtaagagaa attgatcgaa gatgtgacga actaagtaac
     3961 cgaacgggta ataaatggag tcgtaacgat tatttaaagc tactcgttga aaatgatttt
     4021 gatcgtcctt tgatggaata taaacaagat aaatttgatc aattattaaa tcagttaata
     4081 gataaatttt cttttgttca aagtcataat acaaaagtat tagaagaata tatttgtcag
     4141 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
     4201 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
     4261 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
     4321 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
     4381 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
     4441 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
     4501 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
     4561 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
     4621 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
     4681 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
     4741 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
     4801 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
     4861 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
     4921 tagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
     4981 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
     5041 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
     5101 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     5161 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     5221 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     5281 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     5341 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     5401 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     5461 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     5521 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     5581 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     5641 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     5701 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     5761 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     5821 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     5881 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     5941 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     6001 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     6061 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     6121 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     6181 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     6241 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     6301 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     6361 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     6421 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     6481 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     6541 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     6601 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     6661 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     6721 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     6781 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     6841 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcc
//
LOCUS       DANKHJ010000130         6800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000130 DANKHJ010000000
VERSION     DANKHJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(118..708)
                     /locus_tag="PW113_002918"
     CDS             complement(118..708)
                     /locus_tag="PW113_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2527166.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="PW113_002919"
     CDS             1231..1392
                     /locus_tag="PW113_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2527167.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="PW113_002920"
     CDS             1411..1905
                     /locus_tag="PW113_002920"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2527168.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1898..2059
                     /locus_tag="PW113_002921"
     CDS             1898..2059
                     /locus_tag="PW113_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527169.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..3566
                     /locus_tag="PW113_002922"
                     /pseudo
     CDS             2543..3566
                     /locus_tag="PW113_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3909..4165
                     /locus_tag="PW113_002923"
                     /pseudo
     CDS             3909..4165
                     /locus_tag="PW113_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4552..4779
                     /locus_tag="PW113_002924"
     CDS             4552..4779
                     /locus_tag="PW113_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2527170.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4795..5961
                     /locus_tag="PW113_002925"
     CDS             4795..5961
                     /locus_tag="PW113_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2527171.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6196..>6800
                     /gene="hrcA"
                     /locus_tag="PW113_002926"
     CDS             6196..>6800
                     /gene="hrcA"
                     /locus_tag="PW113_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2527172.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIP"
BASE COUNT         2407 a         1009 c         1289 g         2095 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     3061 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     3121 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     3181 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     3241 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     3301 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     3361 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     3421 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     3481 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     3541 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     3601 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     3661 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     3721 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     3781 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     3841 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     3901 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     3961 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     4021 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     4081 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     4141 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     4201 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     4261 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     4321 aaaaataata attggaatct agttaaaact cttgaagtga gagatttgac tagatttttt
     4381 tatgaataaa ataaggacaa aattgtttag gaagaatcat gagtgtaaaa aaatcatcca
     4441 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
     4501 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
     4561 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
     4621 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
     4681 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
     4741 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
     4801 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
     4861 catatctgct attactgtga ctttaataaa gtttttttag aagggcagcc agtagatgag
     4921 tatgtggaaa tgctgctgaa tgaaatgaga ttaatgctgg aaagtcatcc tacagcgaat
     4981 ttggaaacac tttatgtagg aggcggaaca ccaacttctt tatctgctaa acaacttgat
     5041 cgactattat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
     5101 gaagcgaatc ctggtgattt gacccgtgaa aaacttcaag tgatgaaaaa ctatggcgtc
     5161 aatcgtttat ccatgggagt gcagacattt gacaatcgct tattgaaaaa aattggacgt
     5221 aaacatactg cagaagatgt gtatcaaaca atgcaatttt tagaagcaga aaattttacc
     5281 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatcgggat
     5341 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
     5401 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
     5461 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
     5521 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
     5581 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
     5641 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
     5701 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
     5761 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
     5821 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
     5881 gaagaagacc gtgtttattt gacaccaaga gggacttttt tggggaacaa tgtgtttgag
     5941 cgctttttat ttgaaaaata gtttgaagaa tatttgtttt ttatcgaaac ttgaaaagaa
     6001 aaagtttaga aagtttgaca atgaggtatt gaagtattga tataactgga tttacatcaa
     6061 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
     6121 ggagaagggc ttcttgctat atttataagt gaattagcac ttagaatata tgagtgctaa
     6181 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
     6241 cactatacga gtagtggcgt gcccgttggt tccaaaacat taatgtctga gggagtcaag
     6301 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
     6361 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
     6421 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
     6481 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
     6541 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
     6601 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
     6661 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
     6721 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
     6781 cttcgtacgg agattccgat
//
LOCUS       DANKHJ010000131         6732 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000131 DANKHJ010000000
VERSION     DANKHJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="PW113_002927"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="PW113_002927"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL2527173.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PW113_002928"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PW113_002928"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL2527174.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="PW113_002929"
                     /pseudo
     CDS             complement(2837..3872)
                     /gene="iolC"
                     /locus_tag="PW113_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
     gene            complement(4029..5045)
                     /locus_tag="PW113_002930"
     CDS             complement(4029..5045)
                     /locus_tag="PW113_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2527175.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5147..5344)
                     /locus_tag="PW113_002931"
     CDS             complement(5147..5344)
                     /locus_tag="PW113_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527176.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5316..6635)
                     /locus_tag="PW113_002932"
     CDS             complement(5316..6635)
                     /locus_tag="PW113_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDL2527177.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1773 a         1639 c         1159 g         2161 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa agcagcgctt
     3181 tcattatctg acatgttagg ctggatcaac ttctcagtta gatcgaactc ttctcttgaa
     3241 cccatgataa tgtctgattc cttagcaatc atcgaatagt aaattgaaat atcatcgttg
     3301 ttcttccagt tataagcacg gtaatcaata tcaaaaataa ttttgacatt attccttttc
     3361 gccagcatca ctgccttgat tacggcttct ctggacgggc tctctgctag tgcagttcct
     3421 gagattaata gtgcttttga ccgtttcaag tagtcctcat caatatcctc aacgctaagc
     3481 tgcaggtctg cggcttgatt tcgatacatc aaaatattac tttcctttga tgagagcatt
     3541 tcggtaaaag tcagccccag cttttcacca tgctgcgctt tgctgatctg agacgtatcg
     3601 atgccttgtt cattgaaata atcgatcaca tattcgccaa actgatcgtc cgagactgtt
     3661 ccgataaaac cagctttcat gccatgttta gtaacaccca cagcaatatt ggcaggagat
     3721 ccgcccacaa atttttcaaa catatggacc tttttcaatg gtttaaactc ttctttgact
     3781 tgatcattgt aggcaggatt aaaatcaata gcgactcttc ctaaaagcag caagtcaaat
     3841 tcttttgatt ggtcaaactt tatataactc aatatatcca cctctttatc cgttcttcta
     3901 tttctgaggc aagtatagca cgccaaaaca aagcgtgcaa tatttatttt agaacgcaat
     3961 attgcgtgct gtttgaaaat gacggcaaaa aaaacgtgaa tcgccttaca tcacgattca
     4021 cgcctagatt aaatattgta ttttgtctta ataattatat cagtaaatag cgcatctttt
     4081 gtaaccgttt tattattttg cagctgattc gctaacatca gtaatgaacg atagccttga
     4141 acaaatccat cttgatcgat tagaaaatca acctgatcgt ttttcagcat ctcgatattg
     4201 ttcttagtca aatcataaat gattacaaag ggccgcttgc tttcatcgat tttttccaac
     4261 gctcgggcaa ttcccgcttg tccgccagaa aagacgacta cgccatctaa gtttgaataa
     4321 ttctccaagg tgttgcttaa aatcttttca acctcagcac tcgaatcaaa gctgctctga
     4381 acacccgtca gctccagttc aggaaacgac tctttcaatt cttcaacaaa gcctgccacc
     4441 cgtaagctgt tgacgctatt tccaaagtag ccggtaatcg ctagaacctt gccctttcct
     4501 tttgtcagca tacctaacaa tcccgcagcc gtttttccgc ttttgaagtt gtccatccca
     4561 acaaaacact gtcgattcgt accaagaata tccgaattaa aggtaatgat gaggatgcct
     4621 tgatcggtca attgattaat tttttctctg ataccatcac tatctactgg catgatcgcg
     4681 atccctttta ctccaagctc caccaatcga tccaacgcct gcagctgggc cgcttcatcg
     4741 accccgtcta cctcttctaa aaaacaatca atgtttcgct tttttaaatc cccgataata
     4801 gattccagac ctgatcggat cggaatcata aaagaagatt tcgccaattg agtgacaatc
     4861 cccagcttga gcgttgtctc ttttatgacc ttctttttct ttggaacata gttcatttcc
     4921 ttggcgattg ctctgattct ttctgcaacc tcaggattga tccttcctcg atcgttcaat
     4981 gcccgatcca ctgtccccct tgacactcct gctttctcag ctatttcttt aattgtaccc
     5041 gccatcttgc ctctcctttt ttcctattgt gtttatttta tcacaaaact taagaaaacc
     5101 accggagaat gaattctccg gtggactttt tgtatgatta accttcttat tgaacatcac
     5161 tgatcttaaa ctctattcca agggcgtttc tataatattc tggatacatc tccttcccac
     5221 atttttcaca ggtaaattgg ggaggattgt tcggatcatt tgcaatatct gcatcatcta
     5281 aaaattttac tacatccatt gggattgatt ctttttcacc acaacttaga catataaatt
     5341 tcacgttggc gttcccaatc ccagcgaatt gctttaaaag ttttttcttt ttcttcttca
     5401 tactgttgcc tgaattcttt ttggtcaatt tttcgattct cctctcgcat aaaccgcttt
     5461 gcatggttat ctttcgcata tttaataact aaccgtccat ttttgcgcac actcatccca
     5521 acgaattcat agtactcacc gcaacaagaa cattctaagg gattttcacc aaattcttcg
     5581 gttattcttt cttcccagga ttttggtctt aaggcctcat tcagattgat aaacagcttt
     5641 tttactcgtt tctgatactc cttcatgacc tttttcatta atgttttgat tctacgacta
     5701 tagattccat agcgacgaat cgttttgaaa ttcttatttt gaatatggcg aatcagtgca
     5761 gctatgaatt cgtctatact catggtcatc gtctctttag tattcgttcg tttgtccatg
     5821 tagttgaaca tgacagtgtc gccatcatac attaagattc ggcttaatgc gatcgggcca
     5881 cgcttcatat aacgactgat gtattggagt agtgacttta gatttgtcct gctcctttta
     5941 ggagcattta cgtaaaaccc ttctccattg ttcttgtacg cagcttgtaa tcttgattga
     6001 actctttgct tgtcccactc acctaacgtt cgtctaatta gtttcaatac agcatttttc
     6061 cagtattttc ttaacataga aaaaggtaaa taatcgtatt cttcccaact tccatctctt
     6121 gttaagccac ccatagtaac aaccatatga acatgcggat taaattctag ttttgatcca
     6181 aaggtgtgga ttgtagctac tatacctgcc ttcacctttc ttttctcaaa ataggcaaga
     6241 ataatttttg cggcttcatc cattagtcct ttcagtaagg aatctcgata gttttccatc
     6301 ataaagattt cccgtaaccc ctcatcgatc gtaaagatta cgtgccgatg tgtcacttta
     6361 aacatatcat tcgccacaac cgctgaccac ctttggcttt cacctgttgc acacgttgga
     6421 cagaactttc cacgacattt caagggaaca aactttgttt gatgacatcc ctcgcagaca
     6481 aacagtcggt atcctttttg aatatctcca caatatctaa acttgtttac ttcccgtttg
     6541 acgactagcc gaatctttcg cccgtatttc tcaacaaagt tatcccaatt atggtgttcg
     6601 tcaaaaaaga ttgcttcgag aatattcgct ttcatagtaa tattttactt gttcaaaaac
     6661 aaaatgaaaa gggctaaaag actggtagtg cagccttttt agcccttaac tttttatact
     6721 ttctttactg tc
//
LOCUS       DANKHJ010000132         6671 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000132 DANKHJ010000000
VERSION     DANKHJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            2..216
                     /locus_tag="PW113_002933"
                     /pseudo
     CDS             2..216
                     /locus_tag="PW113_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            253..615
                     /locus_tag="PW113_002934"
     CDS             253..615
                     /locus_tag="PW113_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL2527178.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            661..987
                     /locus_tag="PW113_002935"
     CDS             661..987
                     /locus_tag="PW113_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527179.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            1830..3653
                     /locus_tag="PW113_002936"
     CDS             1830..3653
                     /locus_tag="PW113_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2527180.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            3667..5076
                     /locus_tag="PW113_002937"
     CDS             3667..5076
                     /locus_tag="PW113_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2527181.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            5094..5930
                     /locus_tag="PW113_002938"
     CDS             5094..5930
                     /locus_tag="PW113_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2527182.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            5995..>6671
                     /locus_tag="PW113_002939"
     CDS             5995..>6671
                     /locus_tag="PW113_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527183.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGK"
BASE COUNT         2167 a         1045 c         1455 g         2004 t
ORIGIN      
        1 catgaaaaaa gtaagtaatc tcagccgata cagaaaagaa aaaggacttt ctcaaacgga
       61 gttagcaaaa aaatgaatgt tacacagcaa tgtgtaagtt cgtggcaaac agggagaacg
      121 attcctaaac cctatcaaat gaagatgctt tctgagatat taagtgtacc aatgaatgag
      181 ctgttttctg atgtatttaa taaagtaaac agttaactaa taaacaattg aatgttttta
      241 ggaggagaag acatgtattg tagaaaatgt ggcaaagaaa taacagatga aagtaagtat
      301 tgccagtttt gcggagcact acaagaaaca gatgagacaa ctggagcaga cgcagtaaag
      361 aaaagtactg agcagacaat taatgaaatg aaggaacaaa tcgtacatgg acaagagaca
      421 gggaagctgt atttgattat tggttggatt tctatggtag tagcgttagc atttattcct
      481 gttgtatttg gtgctgttgc tgttattatg ggctacttat atcgagataa agatgaacaa
      541 atgggtacga ttttgatgat agcaggggta gctggagcaa tctttggtgt acttttaggg
      601 atgtcaatgt actgataaga gggataagag caaattattt tggagatagg agataaaaag
      661 ttgaataaag taaatatgaa caagaagttt atcattatag ttatcgttgt cttggctatt
      721 ataggtatca ttttttcaaa aatgacaggt acatctggat tagagaagtc atcgaaacca
      781 ttggtaacac agattttaca agaacagtac aacttaaatc gatcatgtga tgatgtgacg
      841 attactcatg aaaatggaga taacacatac cgggctaaag ctatattaga caatggaagt
      901 gctatcaaca taaatattga gtactatcca aagaaagacc gtatatatgt ggagatacca
      961 tatgctgagg tgttaatgtt aaattgattt aaatgtatta tgcataccgt ccaaacagga
     1021 actcccaaag gtgtaaattc actagggagt ttttatagtt tcctataaat taaatactaa
     1081 tacaggtgac aaaaaaatat tacaatgatt gtcgggaatt tttatgttat tcactgttat
     1141 aacaatataa aagaaatgaa aaagaaaaaa ttattatgga tatgtttggt atttttattg
     1201 ataattggtg gaattgaaat acttactaca tgtagattag aaataaatca gtttaaacat
     1261 ttgctagtta atttttaaga atactagccc aaatcattct atcgatacct ttatatctcg
     1321 gaatgattga aattggctat tgcttactga tttttaccaa aaaaacaagt atcactattg
     1381 ttgcctatat tgttggaatg ctgtttattc catcctttac tcaccaaatt catcaatttt
     1441 tcccaagagc agaattgttg aagttgtgtg atccgttata tgcatttttg atgctttcac
     1501 gattttggga atttcccgct ggttatgtag gaatggtttt actggtatag ttaatattag
     1561 atatacttat tttagcagta agtatacgca gatataccct agttgatgtg atctaactgt
     1621 tatattaccc tatacggtca aacgaaaaga taaaaaatta aattccctgt tgattgcgct
     1681 atcataatgt gctattattg gattacagca aaataacgtt attggcatgt tactgattat
     1741 gcaggcaaga ccaagacaaa ctaacacttc aatgttgatt ggggcgtggt ttgacttggt
     1801 cttttttact gctttaagga gagagaataa tggattacag agaagtagga agaaatgtat
     1861 tgcaacaggt tgggggaaaa gaaaatgtgg tcagcttaac ccattgtgcc accagactgc
     1921 gttttgaact caaagataaa aggaaagccg atacgaaagc cttagaaaaa acacttggtg
     1981 taattagtgt ggtggatagc ggtggacaat accaagtggt gattggcaat gaagtccaaa
     2041 cagccttcaa ggaaattcaa aaggaagtgg gaacttcaat taaaaagggc ccctcttcag
     2101 acaatcaaga aagtattatt tcaagattga ttagtgtcat ttctaccacc tttacaccga
     2161 tgattccagc tataacaggt gccgggatgg taaaagccat tttggccgtc ttgactttga
     2221 ctggtgtact atcagacagt agtcaaactt acatcatttt gaatactatt gctgatgcag
     2281 ccttttattt tatgccagta ctattagcct atggggcagc cataaagttt gatgtcaacc
     2341 caattttggc gattactgtt gctggcattt tgcttcatcc aaatatttca cagctttttt
     2401 ctactggcga tgccgtttct tttctaggaa ttccggtatt agctgcagat tatgcgggat
     2461 cagttttacc gattatcttt actgtttgga tcatgtccta tgtagagccc tttgcggatc
     2521 gaatttcgcc ttcgtttatt aaattcttta ctaagccgat gctggtattt ttaattatgg
     2581 ggcctttagc gttggttgtc attggaccgt ttggaacgtt gctgaacgat cttatagcaa
     2641 tgggggctga atggattaac ggcaaagcag cttggttaat tccatttttg atgggtggtt
     2701 tgcagccatt tttggtggtt acgggtacag cttgggcaat gacgccaatc gctactatgc
     2761 aattgagcag tacgggttct gaaatgatca atgggccagg aatgctggct tctaatattg
     2821 ctcaatcagg tgctaccttt gctgtagccg cgaaaactaa aaacaaagag ttgaagcagc
     2881 tagctgtgtc cactgggatc actgctttaa tgggaattac tgagccttca ctctatggag
     2941 taaccttgaa gctgaaaaaa ccattagttg cttccatgat tggtggtgca attgctggtt
     3001 tgtatgctgg cttgagcggt ttagtcagat atgcctttgt ttcccctggc ttagcagcct
     3061 taccagcctt tattggagaa aatccaatga atattgttca cgcaattatt acgtgtgtaa
     3121 tcgcctttgt tgcaaccttt atcattgctt atctaattgg atttgacgat acttctgatg
     3181 aatcagagca aacggcaata gccgaagcag tcattgttga aaatccggta aaaggaaccg
     3241 tgtgtcctat ttctgaagtg aacgatgctg tcttttctaa gggactattg ggaaagggcg
     3301 tagccattca tccagaagaa aacagcattt attcacctgt tgagggagaa gtcattacta
     3361 ttttagaatc taaacatgcg attggtctac gttctaaaga aggtattgaa ttattgatcc
     3421 atgtggggat cgacaccgtg aatttaaaag gggaaccctt tgaagtattt gtaaaagcag
     3481 gagatcaggt gtcagcagga caattattaa tcaacgccga tttcgctgcc attagaaacg
     3541 ctggctacga tgatacagtg attgtggtag tgaccaacag tgaaaattac gcaggaatta
     3601 gtgaagcaag cggaagctta aaggaacacg aaccattgat gcaaattagt taaggagagg
     3661 atttttgtgc aatacaaaca gctttcagca tttccagaga agtttttatg gggtgcctcc
     3721 attagtgcct accaggcaga gggtgcctat caggaggatg ggaaacaacc gtctattatt
     3781 gacaagtatc agcatacaga aggggtagct aattttgaag ttgcctcgga tttttaccat
     3841 cactataaag aagatataca gctgtttaaa gaattaggct taaaagcttt tcgcttttcc
     3901 atcgcttgga cgcgaattat ggatattgaa aataataaaa ccaatcaaaa gggaatttct
     3961 ttttatcatc aagtaattga tgaatgcctg aatcagggaa tcgaaccgat tgtcaccatg
     4021 taccatttcg atttacctta ttttctagag gagcagggcg gttggttaaa tcgagcaact
     4081 atcgattatt ttgtggacta tgtacaggta ttgcttactg agtatggtga caaggtgaac
     4141 tattggctga caattaatga gcaaaacacg atgatccttc atcctggagc gattggtttg
     4201 ccaccaagtg gagagctgcc ttcaaaaaaa gaactctttc aaattaatca ccatgtttta
     4261 ttagctcaag ctagagtaat tcacttgtat catcagttga atcttaaagg aaaaatcggt
     4321 ccagcaatca atttaacggc tatgtatcaa gctacctcca gtccagaaga tgcaattgca
     4381 gcccataatt gggaaacatt gagaggctgg tctttcttag atgtagcagt tcggggaaaa
     4441 tacaattatt tgtttgaaaa ctatttgaat gatcgtggat tatatcctaa aattgaggca
     4501 gaggatcagg caattttatc ctccggtaaa cccgatttta ttgcgattaa ttattattca
     4561 actgccacca tcgctgccag caaaaatgac ggatcagatg tttccgctag agcgggggat
     4621 caacagatca tgttgggaga agaaggtgtc taccgggcag ctgaaaatcc ttatgtagat
     4681 aaaacgccat atggctgggt agttgaccct actggactac gattaacatt gaggaaattg
     4741 tacgatcgct atgatttacc tattttaatt actgaaaatg gctatggggc acccgacata
     4801 gttgaggaag atagacgaat taatgaccag gatcgaattg attatctagc caaacatatt
     4861 ctggctattc aagcagcttt aacagatggt gtagatgttt ttggttattt accttggtca
     4921 gcgattgatg ttgttagtac ccatcaaggc ttcaataagc gttatggttt tatatatgtt
     4981 gaccgaaccg atgagaatct taaagaatta aaacgaataa aaaaagaaag cttcttttgg
     5041 tatcaaaaag taatcgcaag aaatggctta gcttagagag aacggtgaat ctaatgagag
     5101 taattaaaac gttaaacaac agtttaattc tttgcttaga taagcaaggc aaagaagtaa
     5161 ttttgatggg gaaagggatt ggatttcaac taaagaaagg cgatttggtt ccggctgaca
     5221 aaatccaaaa agtatttaaa ttagattcac tttctgatgc tctaaaaaag gattacatta
     5281 aactgtttga ggctgctccg gatgatctaa ttatttgcgt caagcgaatt atcgattatg
     5341 ccaacgccaa gtttgacttt aagctgaata aaagtctatt ttttaccttg gttgatcact
     5401 tgaagcatgc cattgaacgc agtcaaaaag gcattgtttt tcaaaatcgc ctgctgattg
     5461 aaattcaaaa gtattaccca aaagaatttg agatcggctg ctatgctatc caattgatca
     5521 acaaaaaaat gaatgttcag ttgccagaag aggaggctgg aaacattgcg tttcacattg
     5581 taaatgccca aagtgaacaa gcaaatatgg aagaaaccat gctctcggta agagcactaa
     5641 aggatatctt ggctatgcta ggatatctgt ttcccgaaaa aaaggttgat aaagattcga
     5701 tgctttactt aagatttgtg acgcatttac agttttttat tgcccgaatg attaagaagg
     5761 aagagttgcc ggagaaggat gattttttgc tggcttcagt gaagcagcgt tttccgaaag
     5821 agtatcgggc ggccttgaaa atcaaggagt acgttcaaga agagatgggt gcctcagcaa
     5881 atcaggacga actgctttat ctaacactgc atatttcaag ggtgtactga atgaagaaaa
     5941 tatcggctgc acaatatata aagacttgtt ggttgtgcta aggctcctga ttccttggaa
     6001 aattcttcta acagtgcgga ggtgaatgtt tcgacctcta ccagtgaagt ggtaaagtgt
     6061 tcacaagaaa ctactagctt gccagaagag ctacttggag attggcagat taataataag
     6121 gatgctggta tagagatatc agacagtaaa gctgtattcc gtataggaag gtctcaagta
     6181 gagtcaaagg attattatgt agatgtaaac aacaaaatgc ttttacatgt ctatttttct
     6241 tttgagtatg atagtgtgaa atacattgaa gtctctattg aaagaactgg ttatagtgaa
     6301 tttattagta aaagcgtggg aattttaact atgcacacta aagatggaca atatataagt
     6361 ttgggtgtga agagagtttc cggtgagaat aatgagacag aagcagttac ggagcagacg
     6421 atggatgaaa gaatcgattc atctaagagt aatgagacag aagtagatac tgggaagacg
     6481 atagatgaaa gaattgattc attcatcgaa acatacacag ctataccacc tacttggaag
     6541 cttgtaaatg aagataaagg aatcacgata aatactgata cagagatttc tacagatgct
     6601 caatctttat cagatttatt agctaatcta acaggtatca gtaatgcagt gacagagcaa
     6661 gttggtaaag a
//
LOCUS       DANKHJ010000133         6644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000133 DANKHJ010000000
VERSION     DANKHJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(1..859)
                     /locus_tag="PW113_002940"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="PW113_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="PW113_002941"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="PW113_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2527184.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="PW113_002942"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="PW113_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="PW113_002943"
     CDS             complement(2143..2373)
                     /locus_tag="PW113_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDL2527185.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="PW113_002944"
     CDS             complement(2603..3421)
                     /locus_tag="PW113_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2527186.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="PW113_002945"
     CDS             3582..4271
                     /locus_tag="PW113_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2527187.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="PW113_002946"
     CDS             4285..5787
                     /locus_tag="PW113_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2527188.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="PW113_002947"
     CDS             5800..6276
                     /locus_tag="PW113_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2527189.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="PW113_002948"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="PW113_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DANKHJ010000134         6387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000134 DANKHJ010000000
VERSION     DANKHJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            330..1175
                     /locus_tag="PW113_002949"
     CDS             330..1175
                     /locus_tag="PW113_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2527190.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="PW113_002950"
     CDS             1456..2676
                     /locus_tag="PW113_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2527191.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="PW113_002951"
     CDS             2673..5393
                     /locus_tag="PW113_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2527192.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="PW113_002952"
     CDS             5377..6321
                     /locus_tag="PW113_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2527193.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DANKHJ010000135         6230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000135 DANKHJ010000000
VERSION     DANKHJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            133..633
                     /locus_tag="PW113_002953"
     CDS             133..633
                     /locus_tag="PW113_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2527194.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="PW113_002954"
     CDS             730..2577
                     /locus_tag="PW113_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2527195.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="PW113_002955"
     CDS             2580..3476
                     /locus_tag="PW113_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2527196.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="PW113_002956"
     CDS             3526..3915
                     /locus_tag="PW113_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2527197.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="PW113_002957"
     CDS             3902..>6230
                     /locus_tag="PW113_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2527198.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag caaagcaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DANKHJ010000136         6224 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000136 DANKHJ010000000
VERSION     DANKHJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(207..1169)
                     /locus_tag="PW113_002958"
     CDS             complement(207..1169)
                     /locus_tag="PW113_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2527199.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PW113_002959"
     CDS             complement(1171..2610)
                     /locus_tag="PW113_002959"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2527200.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PW113_002960"
     CDS             2795..4741
                     /locus_tag="PW113_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2527201.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="PW113_002961"
     CDS             5005..5877
                     /locus_tag="PW113_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2527202.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DANKHJ010000137         6156 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000137 DANKHJ010000000
VERSION     DANKHJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..2422)
                     /gene="esp"
                     /locus_tag="PW113_002962"
     CDS             complement(<1..2422)
                     /gene="esp"
                     /locus_tag="PW113_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL2527203.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAK"
     gene            complement(3039..3473)
                     /locus_tag="PW113_002963"
     CDS             complement(3039..3473)
                     /locus_tag="PW113_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527204.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3490..4695)
                     /locus_tag="PW113_002964"
     CDS             complement(3490..4695)
                     /locus_tag="PW113_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL2527205.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5389..5775
                     /locus_tag="PW113_002965"
     CDS             5389..5775
                     /locus_tag="PW113_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2527206.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5869..6060
                     /locus_tag="PW113_002966"
     CDS             5869..6060
                     /locus_tag="PW113_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527207.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         1996 a         1096 c          848 g         2216 t
ORIGIN      
        1 cttttgcttt caacactgga agtttatctt ctggcaagct tacgccgtct ttcactgcaa
       61 agactgttgt tccactttca atacttgttc cttcccctgc ggtgaaggtt actttgtggt
      121 agcctgcagg aatgttttct cctggatttt cataaatatc tttacttaca gttactgcta
      181 aatcggtcgt gcctacagaa ccatcttggt aaacaatttt aataggaatt gttccagtaa
      241 tcggctccga ttctcctaca cctttagctg gagtgaatgt aacaatacca gtgtttgcat
      301 caacacttgc attaccgaag tacttattat agacatctga aattgaaaag gcatctttag
      361 ctaaaggaac atctggttta tcaaatgtag gttttccact ttgaactgtc gtttctggag
      421 ttgttgttat aggtacgtat gttgcatcat tttccatagc ttgagaagct tttacagcaa
      481 actctacatc cacgtcgaaa gttcgatttc cgatcttaac agttacaata cctttcgtac
      541 tatcagtaac gttagaagta tcaggctctg ttttccatga atactctgca tcttcaggta
      601 agtttttatt attccgaatt gattctttag catctggttt aaatccaact accacggttt
      661 gtttatcaac cgcttttggt gattccttaa taacggttga accttcttct ggtttatcaa
      721 aacctggaga aacgatttgg atttttaatt catctttcgc gattaattta cttgaatcta
      781 cacccgtaaa ttcaaattct acagtatctg aaattggagc cccatctttt tcatttggag
      841 cgatagtttt ttctgctaat acagtacctt ctttatcaac aagttgggct ttgtgacctg
      901 ttcccgctaa ctcgtggatg aatactttcc ccttaactgt tgtgtcaaca tcatatactt
      961 cttctaattc aggtttcccg atatatggat tcgtgtctcc gctctcttct ttatcgtcag
     1021 ggtttccatc accatcaata tcttgcaaat aataataacc tgttccataa gtgttctgga
     1081 ttaatttttt attcgtatcc gacaaccaag aatcaaagat gaaatcttca ccaaatggtt
     1141 tattttcttc aacttgtgct gttgctttca tatcttctaa gatttcatct aatggcttag
     1201 tcaattgtaa aacatatcga ataacgactg gacgtggact tgcattagca aaatcgataa
     1261 gtttagttaa atcattatta gttattgaac ctatgccata attttcatga ccttcccgtc
     1321 gctcgattgc taaatcagct acttttgttc cggattcaaa cgaaacatca tatgttactt
     1381 tgtcagcctg aaccatgtgc atctgtatac ccgctacata tggaactaat cgttcatcaa
     1441 ttttgtaatt agcataccaa ggcgtgccct ttgcggaaga tgaatatgag aaattataat
     1501 ttttcgttaa cttatgatct aagattatag ctccattagc acctgtggat cctgaaccat
     1561 cttttgtttg agtattaggt ttctcatatt ttgcacttac tgatccatgt ttaaactggt
     1621 caggattatt ttcagataca tctaagctat tatagaaatc atctgaatct gttaaaaagt
     1681 agcccgtact atcaataccc ggtacaattt tatcatcgtc ttgatgactt accaagtaga
     1741 ttcgatacat taaacgatcg gtcttgagat tgtctatgcc tatctcttta aaaatttcat
     1801 ctaggctctt ttcaaacgta attaccccat taggagcggt ttgcgtatcg gttgtttctg
     1861 ccccagcaaa taatccatca cttgcacgaa tataattgac cttccaaata tttgtaggtc
     1921 tacccagcga atcattaatt cttacaaagg tccgtctaga agatccaatt cctttctttg
     1981 gatcgacctg aattttttct acatatttag ctaatcgttc atctaattga agacggattt
     2041 cccagttttt agttgatgct gcaatattat gttttccgta aacttcaaat ccaattgtat
     2101 tgccttttag tgtgtctcca gttagttctt gaggactaaa ttgtaaatcc gaaaagttat
     2161 aatttttcgt ataaccagga ttctcctgaa aagtattccc cgaagctact ttgtcaccta
     2221 cttgtacgga gcttatcgct gtttcaagtg ggctgcttgg agaaatttca tcttgtgctt
     2281 taactagttc agttgttccc aagacaagac caacaccaat caaaacagat gcaaccccaa
     2341 cacttaattt tttgatactg tacttcaata ctcgtttctt tgttttttct agaaatactc
     2401 tcttattatt cttgctaacc attattcaaa tcaactccct taataattac tttcctagta
     2461 agaataaaag ataaatctga tacgtattat tcacagaata ggaatcttac taaccttatg
     2521 ttaactaatt tacttagaga atttcatgaa cattacaaca aaaaaagtac atttcttcat
     2581 gtttttttcg gatatttgtg ctttttaacc attcagcagc tgaaaacata taaaaagata
     2641 tgtatgtatt ctttctgaaa ttaagttcat ttctatgcga tgaagcatgt tttcaaaata
     2701 ttaccctttc ttagattaaa cttcagagta ttcaaataac ggaataagga gccttatccc
     2761 gcattactga atgagactgt gacataagta aaaaggtgac ttcaaattcc ttcttcggaa
     2821 tttgaaatca ccttctttat aattataaag tatcggtttc acagcatttc tgaatgattc
     2881 ctagaatctt aaacgaatga ttggagttat caacattttt tcactcgttt ttagcgaaac
     2941 aaaaaatgac atttgtcaca gcctcattaa attattgatt ctttttcttt ataattgaat
     3001 tgtccatatt taaagtgtct agtactctac atgaataatt aattgctttc actcaaatac
     3061 ttataatcta ttaacttgta gttgtttaac ttttctgaaa aagttagacc cacgtaataa
     3121 tcttcccagc tttctctttc aattaactga tccaattttt gataatccat aaattcatca
     3181 tgtatcaaaa agaataaata acttgatatt ttagcatcta gcttaatcag attaaataat
     3241 gtttgtttaa aattgctacc attaatatga ctgatagatt ctctagcttc ttcacataaa
     3301 ctttttactt tattaattac tactttgtca tattcattta acacactatt attaccatta
     3361 cttagacgct cagattctac gaaacaactc aaatcagaaa tttcccaaaa aaattttttg
     3421 ttaaaatcca atttcctcaa ctcctccaaa ctacgtaaat gatatagtct cataataaaa
     3481 taaaacctct cagctaatgt tgttgaaatt gtccactaaa cctaattgat attgcttagg
     3541 agatacacct gcgtactttt tgaatactct cgaaaagtgt gaagaatctg aaaatcctaa
     3601 acctaaagaa acttcactga tagtatggtt tgatttcaac attattttag ccgtagaaat
     3661 ttttcgctta gagatataat tataaatcgt aatatgcatt tcattcttaa agctttctct
     3721 taatttagtt tcacttacag aaaaataatt agctatttcc tttacagtaa ttcttttata
     3781 gatatttaag ccgatgaatt gaacgacaga agaaattaag ggagaaagat gctctatctt
     3841 agtttttccg atttccttag tataataaac aatcccagaa tccctcactt ttaagacctc
     3901 aggtaacgat acagctaatt cgttttcttg tatcaaagca tctctacttt gatacgcttc
     3961 tattatatcc attcctaatg ttatagacgt ttgtgctaac ttttcaaaaa caatgataga
     4021 ataatttttt tcagaccgaa tcgtatctcc actaacactt ggaattattc cattactcag
     4081 attaaaaacc atgttttcta aatttcgaat atctccattt ttaacatatt ccagtatttt
     4141 attttcataa agaaacagat atatttctgg atcgtaattt tgtgataaca gattatctat
     4201 tttgatttgc tcgatagttt tacttagatt ttctgtatat tcaattaacg gttcatggaa
     4261 tagatcttct atttttccag taaaaaaata atgaactaaa atcagaatat ctcgaatgtc
     4321 tcctaaagaa tacaaagtca aattttgcaa tgaattatag agtcgttctc tatcagccat
     4381 tgcaacattc aaattgttca tttttagttt aaaaaaaact ttatcaatat tatttgctct
     4441 aaatggacca aataagtaat atctggtttt aaacatgtat agaagaaaca tctcattaaa
     4501 atcaccagta aagaaatgga aatctttagt ttttttaata ttatcagtta gtaacttttt
     4561 ataatcataa aaaaaataag tagttctatc tgagcgatac tcttttataa tactgaattc
     4621 ggaattaaaa accacgatag gtaactttaa acaaacatga agagacttta ataagacttc
     4681 tttatctgaa aacacttttg tttctctctt tcctttctac atcattaaat agtattttat
     4741 acgttatcta aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat
     4801 ccacttatcg ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca
     4861 tcatttttca tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta
     4921 tgatccgtta ttatctaagc actatacaga taataggaat ttttcgtttc taaatattcc
     4981 aatttgggaa tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag
     5041 ttgttaatta tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta
     5101 tccaaaagga ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag
     5161 ccataaggac atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga
     5221 taactatcca ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa
     5281 tgcatgtgag cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt
     5341 gttttttgcc tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac
     5401 gacgaacgcg taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct
     5461 aatacttatt ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca
     5521 ccatatttgt ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg
     5581 actctccttg ttgaagatat aaaattagca aaaatccttc ctttgttgaa agaacctgaa
     5641 caaatttttc ttatcgaaaa atttatcttt gataaaactg ataaagaaat tggaaaggtt
     5701 ctggggatta caagacaggg tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa
     5761 aaaatgctgg tttaatatct gcataataac taattttgta tgcttctttg ctcgttctcc
     5821 tccttcccaa agaaatagat tataagtcca tgggaagaag gtagatttat gaattcttta
     5881 aaagaagatt tcattttagc aaaggctgga aatgaagaag ctgttgaggc aattctcaaa
     5941 agatttagct ctttgattca taagcaatcc tggagaactg gaaaatatga tcaagattgt
     6001 tatcaagaat gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa
     6061 ctcaactaag ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat
     6121 ttgaagccat cttctttata attataaagt atcgat
//
LOCUS       DANKHJ010000138         6115 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000138 DANKHJ010000000
VERSION     DANKHJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(52..741)
                     /locus_tag="PW113_002967"
     CDS             complement(52..741)
                     /locus_tag="PW113_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527208.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(748..1026)
                     /locus_tag="PW113_002968"
     CDS             complement(748..1026)
                     /locus_tag="PW113_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527209.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(986..1900)
                     /locus_tag="PW113_002969"
     CDS             complement(986..1900)
                     /locus_tag="PW113_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2527210.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1882..2262)
                     /locus_tag="PW113_002970"
     CDS             complement(1882..2262)
                     /locus_tag="PW113_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2527211.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(2712..2999)
                     /locus_tag="PW113_002971"
     CDS             complement(2712..2999)
                     /locus_tag="PW113_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2527212.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3019..3243)
                     /locus_tag="PW113_002972"
     CDS             complement(3019..3243)
                     /locus_tag="PW113_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2527213.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(3698..3868)
                     /locus_tag="PW113_002973"
     CDS             complement(3698..3868)
                     /locus_tag="PW113_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527214.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(3883..4416)
                     /locus_tag="PW113_002974"
     CDS             complement(3883..4416)
                     /locus_tag="PW113_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2527215.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(4437..5381)
                     /locus_tag="PW113_002975"
     CDS             complement(4437..5381)
                     /locus_tag="PW113_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2527216.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            6018..6104
                     /locus_tag="PW113_002976"
     CDS             6018..6104
                     /locus_tag="PW113_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2527217.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT         1616 a         1134 c         1029 g         2336 t
ORIGIN      
        1 tctaatgttt gatcatttgc atgtttagtt taacatgatt gccgatcaaa ttcaattttg
       61 ctgttgctga tctaaccatt ccttgacact ttttagataa tttaatgccg tttctttttt
      121 cgttggattt tgtatgttcg ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac
      181 tgattgttgc cgttcttttc gttcctgttc actagttgaa aaagggattg tatcactgaa
      241 taaattataa caagcttctt ctgtctgcgt atctttaaaa tattgcacgt cttttttggt
      301 tcgctcatag ttgtcttgaa cctctttttg ctgtttttct ggcaagtttt gataaagacg
      361 agtgagtttt tccatgtttt tatccgtcaa acgtttttct tgattcacaa aaatatcgtt
      421 catttcctca taacaagtgg taaactcttc cattttttgt gttaacactt gctccttttt
      481 ctttttatca catgctgctt catacttttg agtgatatcc cctaataaaa tacttcttga
      541 aatacgttta tttccctcta aaacgccgaa taaaccactt aaaacgattg ataatgtaaa
      601 tataacccct ggcaataaaa acctcttaaa tcgaatttta gagcctttta atgaatatct
      661 tgtttgataa gccttatagc taagccaaat aattcctaaa atactggcaa aaatgagtag
      721 atagggtaaa tagaatgcca tttctattta ttctccccat accagccaaa aggctaacag
      781 tgtcatgatt aagcaacata agggggcaag aatatccatt gatcgctgca taattacttt
      841 tttctgttct ttatttcgct ggtttaatag cgcatggtgg aattttttga cttgtgaatc
      901 aattgtagtt tgtattcttt ctttggtata aggtgcaaga aaagcacgtt gtttctttat
      961 ttcattttct atgtcttgta acgttttaga acgataattc ttgttggtag tctgctcctc
     1021 tttcaacgtg ttggtcatgg tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa
     1081 gagttcgttg ttctcttcgt acttcttttg cagtttcgcg aatgctagct cgagctcttc
     1141 gtagctcgtt ggttcgttgt tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa
     1201 tcgtctcctt ttcgtaatcc tttcctagtt tgttggatcg tacttttttc tttttgtgga
     1261 tcaattcgta agtaaaattt ttcccacgat tcaagcaatg aatgccgaat gttttaagag
     1321 aatcacaaaa ttggttatac gttcgagttt ttttgttttt cattgtttga tcaatcgcaa
     1381 accgaatttc gtctttccaa acaggttcat ttttttcttt taatttgatt tcagacatgg
     1441 ttcgtttttc gggtttcttc cctcgttcag tgattgttaa gtcatgttca agacaaatct
     1501 cgtcatttag gtcataacat ttgtttaaaa atgcttgacc atgggcttga aatttgttcc
     1561 cagtttccag attgacggag ttaatgacaa tgtggttatg aatatggtct ttgtctgtat
     1621 gtgtgtaaat cgcgatttgg tggtcagggg ctattttttt cgcatactct gcccctagtt
     1681 gattggcttt ttcagcggta atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt
     1741 gtacacgatc gtcttttcca tacagcatgc gaatttgttt catttcgctt ttggcataat
     1801 caatgtcaca gtttatccct gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc
     1861 ggctacaaga agtggttcgg cttactttaa ctgttgccat atcttttgta gctccttttg
     1921 catgattttt aggttttcgg ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg
     1981 tgtgttgcac cacttggcaa tttgattgat attcccgcca gctttggcta aatcgcgcgc
     2041 gattgcctga gcaatgtctg gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt
     2101 ggctttcacg aaagccggca cactcatttg caaagcttca gctgactgct tcagctttgc
     2161 aaattcggat tcgctcactc gaaaactgat ttgttttggc tcggatcgat aggtatattt
     2221 tttagaatga ttgctagcca aatattgatt ttgttcgctc ataagtccca ccctttcagt
     2281 acgtcctacg gacgtatcgt tcgcaatacc attgtaccta aaaaggtaca ataagtaaag
     2341 ctcactaaag ctcactaaag ggcttggcaa gctctatatt tgctagccaa atacctacgt
     2401 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc
     2461 cccactctcc acaatacaaa aaagactagc aaatgctagc cactttttgt aaagtttcgc
     2521 ttgtttcacc ttcggtgaaa ttgttgcaaa tcaataatta ggtagagcta catttttttc
     2581 gttgttcaaa gattcttatg ccatttacta ttaataaatc tataaatatg ataatttttc
     2641 ggctctataa tttatgggac tgatgaatca gaattgattc ttcagtccca ttttcatttt
     2701 attccaaaga attaactcca aataccaata gaagcccatc ctctatatac ttgagattcc
     2761 ttactaacaa tttcttgtaa ttctattaaa gatttaggta atccaaattt atgttttaaa
     2821 atatatcttc caagaaaact acttaatttc atactaactt caccaacact ttttccctca
     2881 ctgatttctt tagaataaag aaagagcttt tcttttaagt caggatagtc atctatagtt
     2941 gcttcattgt atactttact aattgcattt aaaagttttt cttctttagt aaaatccatt
     3001 ataataacct ccctaccact accttctagg tgcccatgga ccatgattaa cccaaccatt
     3061 aacaataatt tttccaattt ctcctttagc ttgattccaa tctacccaac atttttcttt
     3121 gttacaataa agaccatttc catagtaagt agctgcatca acttttattc ctgtaccgat
     3181 gccagctaga caagttccta atattcctag aataacacaa tgttttaata ctttcttttt
     3241 catgattgtt tttccttttc gtttttttaa aattgcttgt tatattaaat aatcttagta
     3301 attttattat ataacatttt attatataac aatgattaac gtcataaaaa gtagttttga
     3361 attcataatc gtttaaataa aattataaga tcagttattt aaacagctag tagacataat
     3421 gtgtatacta ccaagtaacg cctgtaccaa gggatccagg cgctgatttt gtgaattttt
     3481 attggtctag gttaaaagct ctttttaagg cttggttttc tgcggttgtt tctttctaat
     3541 tctttgtgaa tattgatcgg tcatttttta aatacttgtt atttttgttt gtgtttgtta
     3601 tgctttttac atatctcata ttcatttatt attcttttct aaggttaata ataggataat
     3661 ctctttcaga aagactgctc gtacgagcag tcttttctta tttatcaagt ttttctacaa
     3721 atacttcctc attttgactg ttgtagtaat taggattgag ttttagttcc ccttttttat
     3781 ttcctttgtt ggccactgat aataacattg ggctaaagct taatattcct agtaggatag
     3841 aaccgaatag caagtgtttt tttgtcataa gtatttcctt cttcatttcc atagctgcca
     3901 ccattttttc caattctttg tttgttcgtc cattgtttct gttgcttcag aaagagtcgt
     3961 tgaactattc tctgaagact cttccgtata agtcaactgc agttgctcct gaagtttttc
     4021 gatttgttgg ttggcttgca gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg
     4081 agaattattt tgctctttga gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa
     4141 ctgatctagc aacttgtttg caaccacgtt tttgtttggt atataagcat ttttatcgtt
     4201 gttttgttca tgtttatgca acttatctag caacctttga ttgtctaagt tgcgagcaag
     4261 ttgcaatact ccagaacgca atacaacctt ataaccacgc tcatttttag ctatcatgga
     4321 agaatctaat ttttggatat gtttcgatat tgcttgccta ctcatcttaa aaagatctga
     4381 caattccttt attgtaaagg tttcttgtgg ttgcatactg gttgcctcct aggttgctac
     4441 tggaataaat tatctaaatc agataataaa ctttttttct ctttttccga aagtgcggac
     4501 tctgtttgtt tttcaagagc ttttttctct ttttctagtt gtttgatttg ttggaggagt
     4561 tcaagttctc gtttcttctg tttgattttt tcgttaattg ttgtagcggg ttcttcgtat
     4621 tctttttgtt tatcagctaa agccaatgct ttattttggt cttctttggt tggtgcatgt
     4681 ttgttaaagt cgtctgcttt tttgatctca ggtttgaacg taaattgata gccaataaca
     4741 ggttttcccc gtccttttcc gtatttcttt ttgattgtta gtcctttgaa gattgcagtt
     4801 agttcttgac gtataggttt tataactcgt ttatctatat caggttgctt ataactttta
     4861 ggaatatcta ataattcaag aaagtcttct ttggagaaat atgcgaaacc tttggttctg
     4921 tattgcttta acagacgaaa catagtcttt gcatagccac tttgtagctc gttgaattgt
     4981 tgtaaagaat agcgtaccca ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa
     5041 attcgtatat ctacgtaagg attttctacg tccccgttga ttttaaattc tgtaaacatt
     5101 acaaacatat cgtaagaaag cccagactca cttctacgac caaagcgtaa agataagatt
     5161 ttttcataag ttgcttttaa atcttctaca aatcgtacat ttgcagttgc tttgtagtta
     5221 cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt
     5281 ctagacacga tggaaaaaaa caaattcatt tcaaccggtg taaattttct taaaggaatt
     5341 gtatttaatt catggtggta ttttacaatc tcattagcca tataatcacc tcaatccaat
     5401 tatgaatgac attttttaaa tgtcaagaca ttcatatttg acgttcaaac agacaaaatg
     5461 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataaacagac
     5521 aaaatgtcct tcataacatt ctgtatccat tggtagagta aggacacagc ttacggtaaa
     5581 gaggtttaaa aagaggttta aaaagagata taaaaagagg ggggctaatt ttcccgccta
     5641 aaaaccactt cttttttctt taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa
     5701 ccttgcttcc tacggtcgcc aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca
     5761 gtcgcctaaa ggctcctttg ctaaaaacat gttggtaaag aggcctttga gcggttctga
     5821 atgcttatct aagccgtttt aagacagtct tggataaaaa tccttatcta gctttaaagc
     5881 gccttacagg cggtcagggc aagccctaac aaccctaaaa taagcgatac ttaaaaacac
     5941 cacacaagtt taatttttta agatataata acttgtcgcc tatccgacag tcttgcgact
     6001 ggaaggaggt gtatctaatg tttgaattgt tcatttgccc ccttcttata gaagtttgtg
     6061 tctctttatt cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa taaag
//
LOCUS       DANKHJ010000139         6033 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000139 DANKHJ010000000
VERSION     DANKHJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..439)
                     /locus_tag="PW113_002977"
     CDS             complement(<1..439)
                     /locus_tag="PW113_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2527218.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFA"
     gene            complement(1031..1318)
                     /locus_tag="PW113_002978"
     CDS             complement(1031..1318)
                     /locus_tag="PW113_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527219.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(1315..1524)
                     /locus_tag="PW113_002979"
     CDS             complement(1315..1524)
                     /locus_tag="PW113_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527220.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(1521..1718)
                     /locus_tag="PW113_002980"
     CDS             complement(1521..1718)
                     /locus_tag="PW113_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527221.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(1715..2041)
                     /locus_tag="PW113_002981"
     CDS             complement(1715..2041)
                     /locus_tag="PW113_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527222.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(2038..2319)
                     /locus_tag="PW113_002982"
     CDS             complement(2038..2319)
                     /locus_tag="PW113_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527223.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(2332..2550)
                     /locus_tag="PW113_002983"
     CDS             complement(2332..2550)
                     /locus_tag="PW113_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HDL2527224.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(2547..2858)
                     /locus_tag="PW113_002984"
     CDS             complement(2547..2858)
                     /locus_tag="PW113_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL2527225.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(3035..3349)
                     /locus_tag="PW113_002985"
     CDS             complement(3035..3349)
                     /locus_tag="PW113_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2527226.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(3349..3648)
                     /locus_tag="PW113_002986"
     CDS             complement(3349..3648)
                     /locus_tag="PW113_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527227.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(3651..3812)
                     /locus_tag="PW113_002987"
     CDS             complement(3651..3812)
                     /locus_tag="PW113_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2527228.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(3827..4186)
                     /locus_tag="PW113_002988"
     CDS             complement(3827..4186)
                     /locus_tag="PW113_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2527229.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(4183..5034)
                     /locus_tag="PW113_002989"
     CDS             complement(4183..5034)
                     /locus_tag="PW113_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2527230.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(5051..5884)
                     /locus_tag="PW113_002990"
     CDS             complement(5051..5884)
                     /locus_tag="PW113_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2527231.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            complement(5891..>6033)
                     /locus_tag="PW113_002991"
     CDS             complement(5891..>6033)
                     /locus_tag="PW113_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527232.1"
                     /translation="EYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQETIKKLRI
                     GG"
BASE COUNT         1559 a         1292 c          894 g         2288 t
ORIGIN      
        1 aagcaaattg gatgataatt ctgtttgatt ctactttaac cgattcttcg ctagtattat
       61 tcctttgagc agttacataa atgggcagac cattgatata acgatcatag aatatcttct
      121 tgcgcctttc tgttacatca ggtttatgcg gatgctgaat tgctgaatag ccacgaacaa
      181 ataatttgtg caagtactca aattcttctt gcgcttcctc tctctcaatc atcattcgtt
      241 ctgcttcaaa tgtattattt gctgtagaag gtggaaccaa ggagtaagat gccgttactt
      301 taggctcccg aggttgacct atcctacatc tagctgacag atatgctgaa agaaaaacac
      361 caacattatg ttttgtgcga tccatatcca catcttttgc ctctggtgtc tcatacttct
      421 ttacatcgaa aagtaccatc cattgattcc tccatttatg atataataat tgtgtcagaa
      481 ttattaatta aggtcggagg aatccggctt tttttatacc catgcttatg ctaagctttt
      541 caagtagcga ggttgcactc gttacccata tacatgttga gctatctggc ggaaaacaga
      601 tggctcacta tttcaatatt ctgctaagga cagccagtgg tcggctgtct tttttatatt
      661 ttaatgagaa gccttactta tttttttatc tttattacga aaaatgatat tatctattga
      721 taataaagcg tataggagat ggttgagtga ctatgtggag catgctgtta ttttggattc
      781 ccgtttgtat tggtatcgtc gcattttgtt actttgtcaa acactctaga acaaataagc
      841 tcctcatgtt atctttttta cctatagtat tttttattgt acaaattgtt aaatatacct
      901 atattgaatc gcaagaaata ttcatttttt atgtggtagg tttatttatc tctgtggtct
      961 ttttcataat gatactttcc tatttttata aaaaataaat ttttctctta gaagtacatt
     1021 ttttcgctgt ttatatagcc ttcatatcca ccaatctcgc tacagctaaa ttctctttgc
     1081 ttttcgctaa ccacttatca cattccatcg tgttttcaat acgaatgatc gctgagtgat
     1141 tatagacgtg ttctacatat ccacgaaatg gatagatgaa cccttctgct tcgcagcgaa
     1201 ccatgtcacc gactttgaat tttggtttct tacgtttttt aggattcttt gtcggcatat
     1261 ctagcattaa accgccgata ccatgactac tagcgtaaaa tccgtctttt aatttcatct
     1321 ttctacctcc tcatgatcga cagtgaccag ttcatatact tgtgttaatc caccaagtcg
     1381 tcgtgaaact tggttagctt cttcgaggct atcaaaccat ctcgcacttt ggagtatgtc
     1441 tacaatgaat aatgtgttag cccctataga gcgttcatca cttctataaa atttattccc
     1501 gaactttact acataaatct tcattctgct tcctcctcaa tcgtccaagc atttgtaaat
     1561 ttctctaagc ttattagttc atcttcattt aaatatccgt aaccatataa ggattctaaa
     1621 aggtcttctt ttgaatagcc tctaaaatca agtctgagac cgcttaggat gtatacatag
     1681 gttttatttc cataaaactt gattgtgtat gctttcatcc cacttcctcc tcatcgatct
     1741 ctatctcgac atctctgcct tctaaaatct gtttggtatc aatataggct acgagtattt
     1801 ttcgattttg gcttaaagca atatacggat caatcaaagc aacttctgcg tgtagacgat
     1861 aataagcaat acaatctatt tctaaaaata tcaaacgatc aatagcatca ttaatcataa
     1921 aatctgtaac gttttctgct gtatttaaac catctgcata tttaggcagg acttcattat
     1981 tgatgaactc agctacatgt tttggacata aatcagtcag ttcttgttgt gtatagttca
     2041 tttgctgtcc tcctgattaa tatatttcct caaactacgt atccctttac cacgtttttt
     2101 tgttttgcgc tcaatccacg ttcctgtagt tccccagtag tcaataattc cattatgggt
     2161 attaattgca aattgatgat tgtttggata atgagtaaat tcaaatccca aggtttcaat
     2221 atttttagtt gctgaattgc ccatctttct gacatgttgt tttctacgtt cttttagatg
     2281 cggtttaata tctttccagt attcgcccat atcgcccatt tcgctgtcct cctatctcct
     2341 tgtccacaat aaaaaccaca tcaactcttc tgtcgtaccg tttaaataaa gatttatttc
     2401 tgcaccaatc gcaactaaaa ttccgacaac aaacaaaata atccctactg ctgtatattt
     2461 tttgcctttt gcactctcgt aaatgaggta tgctcctaac atcgctagta agccgccaat
     2521 tacacaccaa aatattgtta ggctgatcat ttgctgtcct ccaaatcact cgacttcacg
     2581 aatacaccat ctaccatttt tcctgtgcgt cctttgattt cgttgtatgc ttggttcaga
     2641 cactcgtaca aatccatatc attttgcata gctaaaataa tcaaggttac tactacatct
     2701 ccaattccgt ctcttaaacc atgttcatct tttctagcta gagaagcggc aacttccccg
     2761 atctcttcga tcgtttttaa catttgcttg ctggaatcag cttgatccaa tcccttatct
     2821 ttagcccact gctctacttt tgtgattagt tcgtccatta ttcattctcc tttatatatt
     2881 taagttggat aatacaactt gtaacaaatg aagctaatgt taaaattgtg ccgattggtg
     2941 ttaaaaaacc gctttttatc gcattaatta atatagctac aaatatcgtt atatagaata
     3001 aaaagtgaat tgacgctaac gttgaattaa acatctagtc ctcctcgaaa tactcattca
     3061 gtatctctct atacttttct acaaatttga aacgatcctg atgaagtttc ttgctccaat
     3121 ttgtttgccg atccagctca cgcatctgat cgaacccttt ttgaatttcg ttgtaataaa
     3181 attcaatgtt tgctgctgct ttccaatgcc tgctacttcg cactcctgct cctgtttcag
     3241 ccatttccaa cttaactaat tccgctcgtt cttttgcttt tttatctttc tgaatcttca
     3301 tcatgatttt cttgaggatg atatcactgt attgtgtaat gagatccatt atttctcctc
     3361 cacataccta aactgtcgcc cctttgaatc aatccataag ctcctagctc tatcccagat
     3421 gatgtttttg ctcagtccag taatttcaga taactgttca gctgtacccg ttactagaat
     3481 tcgatcacca tgccagattg caatttttct cggcgttttc cgtttaggct tttcagtcca
     3541 cattgattta ccgagctttt ggacttctgc aactatttct ttgtcttctt gccaattctc
     3601 agaatgtgtc agttcaatga ttcgtttcat tgctgctttc ttacccacgc tcattcctcc
     3661 aatctacgaa tttcccttct taaattcact atgtgcaaat tgattgcctt tctcgccgtt
     3721 tcattgacca tcactgcctt tatccgttcc agatcgtcaa tctcacgttg aaggcttcga
     3781 atacgcattt gaatcacttc ttctgttgtc atgatggacc acctcgttaa aaacgctctt
     3841 ccttgaacgt attccgatat tttttagcta atatcaacgg cacttgatat tgatgacaga
     3901 acaactttgc cttgatctta aagtcttttg tctgcattcc tttgacatct acgactttga
     3961 caagtttgcc gtttttataa aatgtgaagt cgggaatata ctcgatcttg cgatactttt
     4021 ttccatctag ttcaaatttc ggcatcagct caaatctttc ctgaagtttt actttccagc
     4081 cgttcgcttc tgcttgccac aaggctaaat cgtaatactc tgcttccgcg atagaatcaa
     4141 acttgatacc tcgatgaaca gttttcttat tacggtattt attcatgcga tactaccttt
     4201 cactggtttt atgcgcttgt ctgctgtttg ttggaatttc agcgcataac cttctgaatt
     4261 cttaaatatt ctagaaacaa ttctttcgcc gtaggcttct cttagttcag gaccggataa
     4321 gtttgttgtg atgatcgttg ccttgttctg tctggcttct aagagcgtgt ttaacgtgtt
     4381 gtttgtaaac tgcctactat ttgatacccc gctacctaat tcagctccaa tatcgtcaaa
     4441 aaccaccaaa tcagttgttt tgatatcggc tataagcgat ccttcaattt cttttctcag
     4501 ttcagcattg ttataagaaa actttatttg ctctaataac tcttgatagc ttataaaaag
     4561 tattttcttg tcataatttg agcgctcaag tatttcccaa gctgtcgcca ttgacaagtg
     4621 gctttttccg cttcctgatt tccctgatag aatgaaatgt gcaggatggt tcagtaagac
     4681 atcatttaca aagcttttag ctctttctaa agcaattttc gtttcttggt ccactacgtg
     4741 ataattctcc attttgcatt taaacaaagt tttatctgtt aataccgaac cattttgaaa
     4801 aaaactcaac gctcgtgctt ttaagctgtc gttatatatc cgttcggtct gtatatcctc
     4861 tttcacacgt aacgctttat aaccacaact catgcatgtt ggtttacaac gttctgaacc
     4921 atccttattt ttagctcgcc aactatacaa aggttcgcta cattctggac atttcccgct
     4981 ttgcactaat actcttctta ttagtttctc catagcattt gctaggcttt ccatgtgatg
     5041 catctccttt ttaaattggc aagtcgtcat attcactagg attgctgtac tgtagttttt
     5101 gactttgctt tttatgatta ttcttgtctg ctttgatttc gaatttgagc ttctcaaatt
     5161 tttctctcaa tttcttagca cttctaatat ttccaaacca aaattcattt gtaggtagcc
     5221 aattgatcac atactcaatc gcttctatag acgctttatc tctttcttcc atcaacctga
     5281 ttgtgtctgc ccatttttcg atatctactt tgttcatttc ttttggaaaa tcttcagtta
     5341 aattactttg caatttttta gcaaggcgta agtgttcgtc agaatactta cctttctttt
     5401 cttctttatc tatatctata tctttctcta tctctatctc taactctggt gtagttttgt
     5461 ctggacattt gtccgacact tgtcctccag ttattaaatt ccgttttgcc tcttctattt
     5521 tctttctgta ttctcttttt ctatctgctt cagttgagga ttttccaatg aaactttgta
     5581 tatcagacat ataaattgct ccgttatcta atacgtcaat aagctgcaaa tcacggaaaa
     5641 tttgtaccgc tttttctacg actcctacag aatgtcttgt aatagttgcg agcattgtag
     5701 agttaaatgg aatcctgtca ttaaacatca acttaccttc gtgttttaga cttcttaaat
     5761 aaagtttgag aagaatatta gaataaatat agccatctgg catactttct aagagaacca
     5821 tctcgtcact atcgaaaaaa ttctctttta gttttaaata gtagtagcgt ttgttgtcag
     5881 acaatatttt ttaccctcct attctaagtt tcttaattgt ttcctggttt aacttgatcc
     5941 ctttgatttg atatttattt ttgaaattga tcacacctat attgtgtttc tccgtgtgat
     6001 gaatcctgca gagtgctgca aatgtgtact ctg
//
LOCUS       DANKHJ010000140         6001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000140 DANKHJ010000000
VERSION     DANKHJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..270
                     /locus_tag="PW113_002992"
     CDS             <1..270
                     /locus_tag="PW113_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HDL2527233.1"
                     /translation="LILDDALSAVDAKTEEAILHNLKEIRKEKTTIIAAHRLSSVMHA
                     KEIIVVDQGKIIERGTHHELLALGGWYERMWEKQKLEAKIEGGEA"
     gene            270..2054
                     /locus_tag="PW113_002993"
     CDS             270..2054
                     /locus_tag="PW113_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527234.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            2512..3363
                     /locus_tag="PW113_002994"
     CDS             2512..3363
                     /locus_tag="PW113_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2527235.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            3460..5346
                     /locus_tag="PW113_002995"
     CDS             3460..5346
                     /locus_tag="PW113_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2527236.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(5387..>6001)
                     /locus_tag="PW113_002996"
     CDS             complement(5387..>6001)
                     /locus_tag="PW113_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2527237.1"
                     /translation="IANKMASLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASN
                     SNTLISQDFMLKMKRIKMDVTIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTA
                     EILKQNHVPILALTSIGDSTLSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLY
                     SCVFAENYQQNLVHLISVGQKFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         1872 a         1049 c         1323 g         1757 t
ORIGIN      
        1 ctgatcttag atgatgcact ttctgcagta gatgccaaaa cagaagaagc tatcttgcat
       61 aatttaaaag aaataagaaa agagaaaaca accatcattg cagctcaccg tttaagcagc
      121 gtgatgcatg caaaagaaat cattgttgtc gatcaaggga aaatcattga acgaggaacc
      181 catcatgagc tgcttgcatt aggcggctgg tatgaacgaa tgtgggagaa acagaaatta
      241 gaagcgaaga tagaaggagg cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa
      301 aatcgattcc acttaaagaa cagaagaaga tcatcaaacg actgatgaag ttcgccaaac
      361 cttttagaag aacgttcatc gttgctatct tatttgcgtt tgccctgtct gtgatcaatg
      421 tcctattgcc aaggatcatc caaactttta tggatgatca tctggctaaa cagtcggcta
      481 cgacacaagt tattttgttt tttgctggta tgtacttgtt tggagtcata gtcaaaagta
      541 tcatttggtt tttccaatgg tatttgtatt ccatggcttc attgaagacc tatcagtatg
      601 ttcgggtaag attatttgaa aagctgcata cactaggtat gcgttatttt gatcagacac
      661 cggcaggatc gactgtttct cgagtgacaa atgacacaga aacgctattt gaattttggt
      721 atgtcttttt gatggtattg actgggatct ttgcagtcgt ttcttccttc attgccatgt
      781 ttcaaatcaa tggagaaatt gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg
      841 tcatctggta ttaccagaaa ttcagttctc gtatttaccg gaatatgcgg gaaaaattaa
      901 gtcagctgaa cacgaaattg aatgaatcga tttccggcat gcgtattatc cagcagtttc
      961 gccaagaagc tcgtttagct aaagaatttg aacaagtgaa caacgagtat ttagacacgc
     1021 gctatgcgat gatcaaaaca aattcgattc tgttaagtcc gatcatcaat tttctatatg
     1081 cattggcgat tgcgttggcg ttgactttgt ttgggatcga tgcgctgcat tctcccgtag
     1141 aagttggact gatctatgca tttacgacct atgtccaggg attttttaat ccaatgtctc
     1201 aaatgatgga ctttttgagc acctttacag atggaatggt tgccggtagt cgtattttga
     1261 aaatcatgga tacagaagaa tatgcaccgc aacaaaatcc tggagcagat caagcgatta
     1321 cttcaggtaa gatcgagttt cgcaatgtaa gcttttctta tgatggagag cacaatgtat
     1381 tgaacaatat ctcttttgtc gctaatccgg gagagaccgt tgcattagtg gggcacaccg
     1441 gaagcggaaa aagctcgatc atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc
     1501 agatcttgat tgatgatcat gacattcgag aatatccaat ggaagagtta cgtaagaaaa
     1561 tgggattggt cttgcaagat gccttcttgt tttatggaga tatcgcagga aatatccgga
     1621 tgatgaatcc aactatcaca gacgaacaga ttcgccatgc agctgagttt gttcaagcaa
     1681 acaagttcat cgatgaatta ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca
     1741 gctattctag cggacaacga cagctgatca cttttgcgcg aacaatcgtg acagatccta
     1801 aaatactagt attggatgaa gcgacagcaa atatcgatac tgaaacagaa ggattgatcc
     1861 aagaaggatt gacaaaaatg cgacaaggac ggacaacgat tgcgattgcc catcggctgt
     1921 ccacgattcg ggatgcagaa ttgattttag ttttagacaa aggtcatata gtagaacgtg
     1981 ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa
     2041 gcagtggtgt gtaaaaagtg gttgtgacag ggctccgttc agctttaggt gataagaaaa
     2101 aaattgtaaa aaaggctttc cagatttttg cataaatttg gcttatcacc gataagctgg
     2161 tactggaaca ccgtatatta gaagttggga cagtagcata gtagcatttt gacctaagcg
     2221 ctagagtgaa aataaaacag agcttcccag attcttcatc tgtgagcgtt agtgtcgtaa
     2281 ttaccagatt taaaaaaaat cacgacagct agtcagaagt gcccaatagc cgctaagaaa
     2341 gcgttgacaa aagtgcttgc tttcgataat ataaagaaaa cgatacaact tggatagtga
     2401 ttgcctgatg caagctaaac ctgatgagtc tcccatggtt ccatggacgg ctttttgggt
     2461 ttttcttttt gttttggata cagccctata tatcatggaa aggagtaaaa catgctgatt
     2521 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
     2581 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
     2641 atcgacaaaa aatttcagtt gacaaatgac ggactttcac aacagttcca agaattggca
     2701 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
     2761 gctctacaag tccagttgaa tgattcggtg tatattgcat tgacggatca tttgtatacg
     2821 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
     2881 caactatttc ctaaagagta tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa
     2941 taccacatca agttatcaga agatgaagcc ggatttatcg cattgcattt agtcaatgca
     3001 caaggcattc aaagccaaca agacaatacc tacctgttta ctacaacaat acaggatatt
     3061 ctaaatatcg tccgctatta ctataagatt acatttgatg aagattcagt ccattttttt
     3121 cgattcgtca cacacctacg tttttttctg acacgcgtcc tagatggaga aaaatcagaa
     3181 gtgggagaag tttctgagga attgtaccag gttattcaaa aaaaatataa ggatacgttt
     3241 ggttgcgttg agaaaatagc agacttttta gccgagatat tcaactacca gatgtccacg
     3301 gacgaaaaaa tgtatttaac gatccatatt gcgcgtttag tgcatgagaa tcgttgtaaa
     3361 taaataaaaa aaggaggata aattaacaag aacgcatgaa ataagggtag tgacattcag
     3421 ttggctaaac cttccactaa taaattggag ggaatagaaa tgggaaaata tcaagaatta
     3481 gcgagcaaaa ttgtagaaaa tgtcggcggg aaggaaaata tcaatggttt gacacattgt
     3541 atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag caaatgatga gatcttgaaa
     3601 aatatggacg gtgttgtgac ggtaatgcga gcgggaggac agtaccaagt tgtcatcggg
     3661 aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag gaaaccttac ttttgatgag
     3721 gctgtttcaa cagaaaaaat gagaccgttt gatatgctta ttgatggaat atcgggctgt
     3781 ttccagccat tccttgctat tctagctgct ggtgggatga ttcgtggact gacagcatta
     3841 cttgtcttct taggcgcatt tgaccgcggt tctggcacat ttgttatgtt tgataatatc
     3901 ggtgattctg tattccaatt tatgcctgtt attatcggtt taacagctgc ccgtaaattt
     3961 aaagtaaatg aattcgtagg gatgttgctt ggggcagcat taatgaatcc tagcttgtcg
     4021 ctggaagctt tgagtggtgc agcagaagca ccactgacta ccatattttc tggcactatt
     4081 tttgaagcac ctatttatca aacagtattc ggcattccct ggattgcgcg taactacgct
     4141 tcttctgtca ttcccattat ctttattgtg ttgttagcta gccaaattca gaaaccaatt
     4201 aaaaagttag tacctgagat gatcgcgaat ttcttcgttc cattcttcac cgttcttatt
     4261 acaatgccct taggtttttt actcgttgga ccagcattca cattcgcaac tgatatttta
     4321 atggctggat ttgaaacact tttagcactt tcgccagtta tttatggagc aatcgtaggc
     4381 ttcttctggc aaattctagt gatgtttggt ctccattggg caatcgtacc aatgggattg
     4441 atgcaatttt ctgtgaatgg ttggcaaaat atcatgacac ctgttgcagt agtgtctttt
     4501 ggacaaactg cagcacttac tgcactctac tttaagctta gaaacccaaa agacaaagca
     4561 atcgcaatac cagccattgt ttctggtatt gtggggatta cagagccagc tatttatggg
     4621 tttacattgc cacgtaaaaa aatatttatt tttacctgtg tcggtggtgc gttagggggc
     4681 gcgtatagtg gattaatgaa tttaacttct tggaatccag gtggattagg catctttaca
     4741 attcccaact atattagacc agatggtgat ttaacggatg tgattaatgt attgattgga
     4801 atcgccattg cgatggtggt atcctttgcg ctgacttttt tcttttggaa ggaggaagca
     4861 ggtgaaacag atgatagaca gaaaagatca ggtaaagaaa ttgtaaaaac accgatacaa
     4921 ggacaaatcg ctccactgaa tgcagcaaag gatgcagctt ttgcacaagg aacactaggt
     4981 cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg ccccttttga cggaacagtc
     5041 atgaccctgt tcccgacgaa acatgcaatc ggaatggttt ctgaagcagg tctggaattg
     5101 ctgattcatg tcggattgga tactgttcag ttggaaggga aatattttga aagcttggtg
     5161 cagcaaggag aaagagtgaa acaaggcgat ctgttgctcc gcttcgacaa aaagaaaatc
     5221 gaagaggaag gatacagttt agaaacacct gtgatcgtca ccaattacac ggattatttg
     5281 gatattttgg aaaatcaagg ggagacggtc gggccagacg atgcacttat caccgcactg
     5341 acctgaacgg ttaggtgaaa gaatggacta ggcttaatta ttctttttag tccacttctt
     5401 tattttcaaa attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcaaatt
     5461 tttgtcctac agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac
     5521 tatataacac gtctagaaga taacaaatcg atgtattgat cgtaaaatta ccaattttcg
     5581 aatacagtcg ttcgcgtgtc gtaagtcgca aagcggctgt actcattgaa gatagcgttg
     5641 aatcaccgat actggtcaaa gctaaaattg gaacatgatt ttgtttcaat atttctgcgg
     5701 tacgaaggat cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg
     5761 tgcaattgta cgcttcatag aacgattcac caaaagtggt gacaatcgtg acatccattt
     5821 tgatgcgctt cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga
     5881 aaatcacgat tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat
     5941 gaatgagaga aagtgtatct tgaatcgtca tctgttctaa agaagccatt ttatttgcga
     6001 t
//
LOCUS       DANKHJ010000141         5930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000141 DANKHJ010000000
VERSION     DANKHJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..279
                     /locus_tag="PW113_002997"
     CDS             <1..279
                     /locus_tag="PW113_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDL2527238.1"
                     /translation="SRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQ
                     VSYEEMTKEEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            289..3105
                     /gene="uvrA"
                     /locus_tag="PW113_002998"
     CDS             289..3105
                     /gene="uvrA"
                     /locus_tag="PW113_002998"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2527239.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            3368..4633
                     /locus_tag="PW113_002999"
     CDS             3368..4633
                     /locus_tag="PW113_002999"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2527240.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            4637..5368
                     /locus_tag="PW113_003000"
     CDS             4637..5368
                     /locus_tag="PW113_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2527241.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            5503..>5930
                     /locus_tag="PW113_003001"
     CDS             5503..>5930
                     /locus_tag="PW113_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adaptor protein RapZ"
                     /protein_id="HDL2527242.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTT"
BASE COUNT         2004 a          971 c         1313 g         1642 t
ORIGIN      
        1 tcaagacgcc gtacgattca gcagaagtac aatgaagaac atgggatcgt tccaaaaacg
       61 atcattaaag aaatccgaga tttgatttct attacgaaag aatcggaaga tgatacaaaa
      121 gaagcggtac aagtctcgta tgaagaaatg acgaaagaag aaaaagatac gttattgatg
      181 aaattagaaa aagaaatgaa aaatgcggcg aaagcattgg attttgaaac agccgctaat
      241 gtgcgagata tgattttaga attaaaagct tcaaagtagg aggacaacat ggctaacgat
      301 aaaattacga ttcacggtgc acgtgcccat aacttgaaaa atatagatgt aacgattcca
      361 cgagataaat tcgtggtcgt tacaggtttg tccggttctg gaaaaagttc cctagcattt
      421 gatacgctgt acgctgaagg gcagcggaga tacgtagaga gcctttctgc ttatgctcgt
      481 cagtttttag gacaaatgga taagccggac gtagatagca tcgacggatt gagtccggcg
      541 atttctattg atcagaaaac aacaagtaaa aatcctcggt cgacagtagg tactgtgacg
      601 gaaatcaatg actatttacg cttgttgttt gcacgtgtag gacatccgat ttgtcccaac
      661 gatcatatcg agattaccag tcaatcagta gatcaaatgg cagatcaagt aatggctttg
      721 cctgagcgca caaagatcca agtgttggca ccgatcgtgg taaagaaaaa aggacaacat
      781 aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc gcgttcgagt cgatggtgag
      841 atctatgatt taagtgaagt gcctgagctg gagaaaaaca aaaaacatga tatcgcgatc
      901 gtcatcgacc ggatcattgt gaaagaaggg attcgttctc gtctatttga ttctttagag
      961 gcggctctgc gattggcaga cggctatgct ctggttgacg tgatcggtga agaagaaatg
     1021 ttgtttagcg aacactatgc ctgtccttat tgcggattta ctgttggcga actagaacca
     1081 agactgttct cattcaatgc gccttttgga gcttgtccag actgtgatgg tttaggtgtc
     1141 aaattagaag tcgatcaaga tttagtgatt ccagatagaa cgaagacact tagtcaaggt
     1201 gctatcgttc catggaatcc aatcagctct cagtattatc ctcaaatgtt agcgcaggct
     1261 tgtgaaagtt tcggtatcga tttagataca ccattcgaag aattaccgga agagcatcag
     1321 gatatcatct tgaatgggac agatgatctt ttccatttcc attatgaaaa tgattttggt
     1381 ggcgttcgcg atgtagaaac gactttcgaa ggggtattga agaatatcga gcgtcgttat
     1441 catgatacga acagtgattt cacgcgtgag caaatgcgtc tttatatgac agaattaact
     1501 tgtcagacat gtaaaggtta tcgattgaat ccccaagctc tatctgttca aatcaatgga
     1561 acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag cagtagattt cttctcaaac
     1621 ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga ttttaaaaga agtaaatgac
     1681 agactttcat tcttggaaaa tgtgggacta gactatctaa ctttgagtcg cgcttccgga
     1741 acattatctg ggggagaagc gcagcgaatc agattggcta cacaaattgg atcgaatctt
     1801 tctggagttt tgtatatttt agatgaacca tcgatcggat tgcatcaacg cgacaacgat
     1861 cgactgattg gctcattaaa gaaaatgcgt gatcttggca acaccctcgt tgtagtcgaa
     1921 catgatgaag atacgatgtg ggcagcagat tatctgattg acgtcggacc tggggccgga
     1981 gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag aagtagctaa tgatgaaaat
     2041 tctttgacag ggaaatatct ttcagggaaa aaatcaatcc ctgtaccaaa agaacgccgt
     2101 aaaggtaatg ggaaagcaat caagatcact ggtgcttcag aaaacaatct gaaaaatatt
     2161 agtgttgaat ttccattagg agaatttgta gcagtcacag gtgtgtctgg ttctggaaag
     2221 agtacattgg tcaatagtat cttaaagaaa tcgttagcgc aaaaattaaa taagaattct
     2281 gctaagccag gtaaattcaa gacaatttcc ggctacgaaa gtatcgaaaa gatcatcgat
     2341 atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc cagcgactta tacaagtgta
     2401 tttgatgata tccgtgggtt atttgctcaa acgaacgagg caaaaatgcg gggttataag
     2461 aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg aagcttgtcg cggggatgga
     2521 attattaaga tcgaaatgca ctttttgcct gatgtctatg ttccttgtga agtatgtcat
     2581 ggcaaacgat ataactctga aacattagaa gtgcattaca aaggaaaaag cattgctgat
     2641 attttggaaa tgacagtaga agatgctgta gaattcttca agcacattcc aaagattcat
     2701 cgcaaactgc aaacgattgt tgatgttggc ttaggttatg tgacgatggg gcaaccagca
     2761 acgacattgt ccggtggtga ggcacaacgg atgaaacttg ccagtgaatt gcacaaaatc
     2821 tctaatggaa agaatttcta tatactagat gaaccaacga caggacttca tagcgatgac
     2881 atcgcccgct tgttgcatgt attacaaaga ttagtagatg ctggtaacac agttttagtg
     2941 attgaacaca atctagatgt aatcaaaaca gcagattata tcattgattt aggaccagaa
     3001 ggtggagaag gtggaggaac gatccttgcg actggaacac cagaagaaat cattaacgta
     3061 aaagaaagtt atacaggtca ctatttgaaa aaaataatgg tataaagagg atagatagct
     3121 gagatagtta aaaaaacata aaaaaatgaa tttttgcttg ttttattaat atcagtgttc
     3181 tagaatcatt acatggtagt tctttctaca ctgattggta tagacaaaaa cttcctataa
     3241 gttcaatccg ttcaaaaaag aattgagcaa tcggaggagt ttcctttgat tgcttttttc
     3301 tttatggtat gatattcctt gtgtagttgg aaaagaaaaa caagagatta tgagtaagtg
     3361 agagataatg atgaacagta ttgaaaatga agaatttatc cctatattat taggaagcga
     3421 catgaacgtt tatggtatgg cacgttcttt taatgaagca tacggcaaaa tctgccaagc
     3481 atatgcttcg gatcagttag cccctacccg ctacagtaaa attgtcaatg tagaagttat
     3541 tccaggattt gataaagacc ctgtgtttat cgagacgatg cttcgtttgg caaaagaaag
     3601 atattctgat aagagtaaaa aatatctttt gattgcttgt ggtgatggat atgcagaatt
     3661 gatctcccaa cataaacaag agctgtcaga atattttatc tgtccatata ttgattattc
     3721 tttatttgaa agactgatca ataaagtaag cttttatgaa gtttgtgaag aatatgatct
     3781 tccgtatcct aaaacattga tcgtcagaga agaaatgtta gtaaatggac atttggaaca
     3841 ggaattgcct tttgaattcc cagttgcttt gaaacctgca aacagtgtgg agtatctttc
     3901 tgtccaattt gaaggaagaa aaaaagcttt tattttagag actagagaag agttcgattt
     3961 aattttaggc cggatatacg aagccggtta taaaagtgaa atgattgtac aagattttat
     4021 cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat gtagatgaag atcatcaagt
     4081 tcggatgatg tgtcttggtc atccactgtt agaagatcct acaccagcat cgattggtaa
     4141 ttacgttgtg atcatgccag attataacga aaaaatctat caaaccatta aggcattttt
     4201 ggaaaaaatc gaatatacag gctttgccaa tttcgatatg aagtatgatc ctagagatgg
     4261 agaatataaa ttatttgaaa tcaatttgag acaaggtcgt agcagtttct ttgtgacatt
     4321 gaacggcttg aatctggctc gtttcgtcac agaagatcga gtgtttaaca aaccctttgt
     4381 tgaaacgacc tatggaacga atcaatcaga taaagcaaga ttatggatgg gtgtgcccaa
     4441 aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa aaactagctg agcaaatgat
     4501 caaagaaaat cgttacggta ctactgtgtt ttatgaaaaa gaccggtcaa tcaagcgctg
     4561 gttgttgatg aaatatatgt tccataacta tattccaaga ttcaaaaagt attttcatgt
     4621 gaaagaaggc tagttcatgg agaatttttt cagtatttta ggcggaatgg gcacgatggc
     4681 gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa gctacaaaag atcaagaata
     4741 tttgaattat gtcttattca atcatgcaac agttcctgat cgtacagctt atattttaga
     4801 tcgatcagaa gaaaatccaa tgccgtttct gctggatgat attgagaaac aaaatttatt
     4861 gcagccgaac ttcattgtgt tgacttgtaa caccgcacac tacttttttg aagaattaca
     4921 agcggcaacg gatattccta tccttcatat gccaagagaa gcagcaaatg aacttgtccg
     4981 tcagcatacg acagggagag tagcgattct aggaacagaa ggaagtatga aagctggaat
     5041 ctatgaacgc gaagtcaaaa atcttggatt cgaaacagtg attcctgata ctgttcttca
     5101 agaaaaaatc aattatctga tctatcatga gatcaaggaa tcggattatt tgaatcaaga
     5161 gctatattac gaaatcttag aagaagcggt agagcgccta aactgtgaaa aagttatctt
     5221 aggttgcaca gaattgtcat tgatgcatga atttgctgaa gataaccatt atccagtgat
     5281 cgatgcccaa tctattttag cagatcggac gattgaacga gcgctagctg aaagaagcaa
     5341 ggcattggat acagcatcgg aaaagtaaaa aaattggatt tggggcaaat atcctaaaag
     5401 atatttggtc caaatctttt tgtaaaagaa aaagaaaact ggtaatgata cgcccggaat
     5461 gatataattt tactatgcac aatgtgagag gagttcgtaa ttatgacaga taatttgcaa
     5521 ttagtcatca taacaggtat gagcggtgca gggaaaacag tagctatcca aagttttgaa
     5581 gatatgggct atttttgtat cgataatatg ccgcctcgat tgattccaaa attctgggaa
     5641 ttaatcaaag aatcaggcaa agtcacaaaa atggctttag gagtggattt acgttctcgt
     5701 tccttctttg aagaaatcca aaacatgctg atcgaaatcg aaaatacgaa cttcatcgac
     5761 acttctatct tatttttaga tgcttcagac gaagaactag tggctcgcta caaagaaaca
     5821 agacgagcac atccaatggc gatggacggt ttgatcactg aagggatacg taaagaacgg
     5881 gcgatactag acgacttgaa agcccaagct tccgtgatca ttgatacgac
//
LOCUS       DANKHJ010000142         5881 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000142 DANKHJ010000000
VERSION     DANKHJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(1..191)
                     /locus_tag="PW113_003002"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="PW113_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            212..1414
                     /locus_tag="PW113_003003"
     CDS             212..1414
                     /locus_tag="PW113_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDL2527243.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2147..3115
                     /locus_tag="PW113_003004"
     CDS             2147..3115
                     /locus_tag="PW113_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2527244.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3126..3941
                     /locus_tag="PW113_003005"
     CDS             3126..3941
                     /locus_tag="PW113_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2527245.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4009..4719
                     /locus_tag="PW113_003006"
     CDS             4009..4719
                     /locus_tag="PW113_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2527246.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4755..>5881
                     /locus_tag="PW113_003007"
     CDS             4755..>5881
                     /locus_tag="PW113_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2527247.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2019 a          884 c         1188 g         1790 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga gaacctagag acggaaatga
      841 tagtatagcg tatgacattg attaccaatt acctgaaggt agtcgtagtc gtattaatta
      901 tgagtattta aacacaaact tcaaaaaata catgaatata aaagaagaac gtgattcgaa
      961 tttaattcaa tcattgttaa acgacttaca tcgcacattt gcgcaattac cagtggagtt
     1021 acagccatat gctgagatga ttctaaatga tgtaaataat ggggaattag ttatcaaaga
     1081 aggatgggaa tttactgatt acctcaatga atatcagctt agtgaagagg acgattttat
     1141 ccaagattta gtggattcaa ccaatattaa tgaggcatta ttacgtgaga tgttggattt
     1201 acgcttaacc gaggtaaaca ttaatgaata tagtcgattt gataaattaa aatcaagtgt
     1261 taacgtaggt catttcagtg gatatatcga gaaaaattta ggtaaaatgg taataccaat
     1321 caaggtaaat atgctaattg acagattatt gcgtgcattt ttattagaag atgtattcga
     1381 tgttacagag tacataaaga attactttaa ctagagtaca cctacatctt atagtacata
     1441 aaaaaattca tcttttttag tttaataatt caccaaaaag gtgagtaatg ccatttttga
     1501 aaattgagct tgttttatgt ctacatctgt atccgtatat aatccaaagg attataaact
     1561 cagaagagtc tttatttttt ctgaatataa ctactgtatc aaaaacaatc gattatgaga
     1621 caaaacaaaa tgacctcttt tatttaagaa aggaggtcat tttttgtatc ataaattgta
     1681 ttaaaatcaa tgtatcactt tttaatcaat tttaagacca taaattgcgt actaaaagaa
     1741 attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc acccatctac
     1801 ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg tgcaaaacga
     1861 tttcttctaa acaaatcaag acaaacttat aaaccttcat cccagcacca taattattta
     1921 aatcataggc atttttcgaa attagtatat gtactcaagt ttcactgcag attatttata
     1981 ttttttagag aaataaattt ttcctattga caaacacaaa gaaagcggat acaatacaat
     2041 tatgtcacat gacgttatat gacgttatgt aatatcttta catttaatca gttactttta
     2101 ttttggataa aattaacatt tttaaaattt taaggaggaa atgttgatgg atgtagcaaa
     2161 aacggttaac gcaattgtag aaaaaaatga tggagagatt tctggaattt atttggtggc
     2221 ttgtggcggt tcgctggtag atatgtatgt ctcagattat tttcttaaag ccgaagcatc
     2281 gaagtgtatt acaggcttat atacagcgaa cgaatttaac catgtaaccc caaagcaatt
     2341 agatgataaa tctgttgtca ttctatgttc tcatggagga aatacagcag aaacggtaaa
     2401 agcaggagaa gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc ataataaaac
     2461 tgcggattta ttaaaggttt ctgatcatag tttcctttac gaatggggag atgatgcaaa
     2521 aatagtcaat aatcctatgg ccatcattct tgatttaact gtatctttgg tcaatgttac
     2581 cgaaacattt gaacattacg gtaaattcag agaaggcatg aaggttatcg ataccgtaat
     2641 tgaaaatggc attaagcagg tgcaggatcg tattaaagtg tttgctgata agtacaaaga
     2701 tgaaacaatg ttttatatct taggtagtgg accatcatat ggacatgctt atggtttttc
     2761 aatttgctct ttgatggaaa tgcaatggct tgattcaact tctgttcatt ctggagaatt
     2821 cttccatgga ccttttgaag taacagacaa agatacgaat tttattctct tgatgagcat
     2881 tggacgcact cgtgctttag atgaacgggc aaaacgattc cttgataaat atgcagaaag
     2941 agtagaaatc gttgatgcaa aggaactagg attggatatg atatcagatg aagtttctga
     3001 attcttcaat cccattttat tctacagtat tctttcagaa tatcgttcag ctttagcaga
     3061 tgcacgcaat catgatttag atgttcgtcg ctatatgggg aaagtggagt actaggagga
     3121 ttaaaatgaa ggtattaggc ttaggtgata acgtcgtcga taaatatgaa aatctgggaa
     3181 ttatgtatcc tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa ctgggacatg
     3241 aaagtagctt tatgggagtt tttggctcag atgaggaggc agctcatgtt ttatcagcaa
     3301 taaaaaaaat aggactggac aacagtcact ctcgctttga aaaaggcgag aatggctgtg
     3361 ccagagtgaa gattgatgag ggcgaccgga ttttccttgg cagcaattcg ggaggtgtta
     3421 ctcgtgaata cccgattcag cttactgaag aagacagaga atatttaaac cagtttgaat
     3481 tgattcatat gggattatat agccatgtca atcatctatt agaagagtta caaaatgttt
     3541 ctggaaagat ttcttatgat ttttctgacg actttacgga agaagaggtt cagcgggcaa
     3601 ttgataaagt tgattttggc ttcttttcta tagaagactt ttccgatgaa gaagtaaagc
     3661 agtttttgat gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa
     3721 aaacggctat tggttatgat ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac
     3781 cagttgatac aatggcagcg ggagattctt tcttaacagc tttcctactt tattttctcg
     3841 aaggggacga tatagtcacc gcgatgaaaa aggggaatga atttgctggg aagtcttgtc
     3901 tggttgacgg ttcgttcggc tttggtattc attacgattg agcttgtttt aaattcctgc
     3961 atttgataac ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat gctggataca
     4021 aatgcacaat taccattgta cttacaatta aaagaaatat taaaaaataa aatcaataca
     4081 ggggagtatc ccgagaatac gaaaattccc gctgaatcag agctgataga aagttatgag
     4141 gtcagccgaa tcacagtgag aaaagcagta gaagaactgg tagaagaagg gtacctactt
     4201 aaaaaacaag ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa aattgaatat
     4261 gttatgggtt tcagtgagag ttgtaaatct aatggtttta caccgactag cattgttttg
     4321 gaacgcaaga tcattccagc aactgaaggg atggcaaaaa aactcgatat cgctcaaggt
     4381 gatgatgttt tatatatcca gagaaagcga atggcggata acatccccat tatgttagaa
     4441 aacaattatt tttcttatga aagatatagt ttcttaatgg aggaagagct gactgattct
     4501 ttgtatgaag tacttaaagt ccaaggtacc ttagccatca atcctgggga aactgcactt
     4561 gaaattgtca cagctgatga ccaaattgcc aaaataatgg aagtagctgt tgggacgccc
     4621 tttttcttta tggatacttt aatcaacgat caaaacaaca taccaattca tgtaggtaat
     4681 caatattacc tgggagaagt atatcgtttt tacttatgat ttcctgtttt gatttcaagg
     4741 agtagatttt atttatgaag caagtattgc ggtcaaaaat gatttttgat ggagagcagc
     4801 cacctttcaa aggttatatt gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact
     4861 ggaattacag tgatctatta aatgaaaaca cggaattaat tgcttatgat gatcaattcg
     4921 ttatgccagc aattcatgat aatcatgtat ttttcagtgg ttatttggct atgaatgctg
     4981 gcatagattt aagcgataca tcttcagcta acgaggctgt agagcgtatt aaagctgtaa
     5041 tgaaccataa aaaagcagat gaaccaattt atgctcatgg ctgggatgct gccctgtgga
     5101 aaactgaccc atcagaagag ctattgaatg aaataaacca ctttggtcca atcacagcga
     5161 tcgacaaaaa tcgcagccat tgttggatga atgctattgc taagcagact tatggtttta
     5221 cagaaaaaga aaccagtgct gaaagtcgag tattattaat taaggaaatg ctagcaaaca
     5281 aaaagctttt agcagaggtt tacgctgaat ttgaagcatt actgctttca aaaggtgtta
     5341 tatcaatgaa agaaatcgtt tttgatgacg cagaatttct ttctaatata cctcagagga
     5401 aaatacttag taacctatat gtgcaggcag tccaagaacc tctacagcat gaacttctat
     5461 atgagtataa ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg aagatcatgg
     5521 tagatggtgt tgttgcggat agaagtggag atatttacgg tgtttatcag tcaggagtag
     5581 cttcacccga gattgactat gaggcaatta aagcagaagt tgctgcattc aatgaagtag
     5641 agattccttg ctgtttgaca acggaaggag ataaagcggg ttataaagct gcgtcaattc
     5701 ttgtcgaatt tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt gatttggaaa
     5761 tggttactca aaagacagca gaaaaaatga acgaaggaca agtagtagca gaggtatatc
     5821 cacaaattct cggcttgaat caaacgttag aagaggctta catgtcaact gttataaaaa
     5881 a
//
LOCUS       DANKHJ010000143         5769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000143 DANKHJ010000000
VERSION     DANKHJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(1014..1304)
                     /locus_tag="PW113_003008"
     CDS             complement(1014..1304)
                     /locus_tag="PW113_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527248.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PW113_003009"
     CDS             1706..1942
                     /locus_tag="PW113_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527249.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PW113_003010"
     CDS             1949..3901
                     /locus_tag="PW113_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2527250.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PW113_003011"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PW113_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2527251.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="PW113_003012"
     CDS             complement(4797..5489)
                     /locus_tag="PW113_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2527252.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1893 a          997 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DANKHJ010000144         5727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000144 DANKHJ010000000
VERSION     DANKHJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            196..921
                     /locus_tag="PW113_003013"
     CDS             196..921
                     /locus_tag="PW113_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527253.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="PW113_003014"
     CDS             911..1420
                     /locus_tag="PW113_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527254.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="PW113_003015"
     CDS             complement(1490..2938)
                     /locus_tag="PW113_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2527255.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="PW113_003016"
     CDS             complement(2938..3654)
                     /locus_tag="PW113_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2527256.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="PW113_003017"
     CDS             complement(3635..3985)
                     /locus_tag="PW113_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2527257.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="PW113_003018"
     CDS             4129..4902
                     /locus_tag="PW113_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2527258.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="PW113_003019"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="PW113_003019"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DANKHJ010000145         5703 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000145 DANKHJ010000000
VERSION     DANKHJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..518
                     /locus_tag="PW113_003020"
     CDS             <1..518
                     /locus_tag="PW113_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2527259.1"
                     /translation="RPAQTNTVVKTETFAGGTIEQDIYAIANGKLIPITEVSDDVFAE
                     KMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAGIEVLLHMGINTVDLKGE
                     PFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTNGDKVQSLEIEPARDVKA
                     NDKIGSVSNKA"
     gene            complement(850..1701)
                     /locus_tag="PW113_003021"
     CDS             complement(850..1701)
                     /locus_tag="PW113_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2527260.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1824..2291
                     /locus_tag="PW113_003022"
     CDS             1824..2291
                     /locus_tag="PW113_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2527261.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(2554..3036)
                     /locus_tag="PW113_003023"
     CDS             complement(2554..3036)
                     /locus_tag="PW113_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2527262.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(3136..3999)
                     /locus_tag="PW113_003024"
     CDS             complement(3136..3999)
                     /locus_tag="PW113_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2527263.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            4597..5472
                     /locus_tag="PW113_003025"
     CDS             4597..5472
                     /locus_tag="PW113_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527264.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(5617..>5703)
                     /locus_tag="PW113_003026"
     CDS             complement(5617..>5703)
                     /locus_tag="PW113_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL2527265.1"
                     /translation="LQNVEKYHKIYQQCRKIYPATKEICHDL"
BASE COUNT         1783 a         1186 c          932 g         1802 t
ORIGIN      
        1 cacgacctgc acaaacaaac accgtggtta aaacagaaac atttgccggt ggaacgatcg
       61 aacaagatat ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg
      121 atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa
      181 tcttctctcc aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa
      241 tccaaacaga tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa
      301 aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga
      361 ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg
      421 tattcacaaa tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag
      481 caaatgataa aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt
      541 tttatcgata aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat
      601 aaaaacggcg ttctcctgcc aactccgcgg taataagtcc agccaaacgt aacatatgaa
      661 gagctatttt ttatttgact cttcttatta cttgatgcag aacagctttt ttacaacctc
      721 tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt caaatttatg caaaaatgtg
      781 gaaagccatt ttcacaaaat ttttcttcat actcaaaact aatcggcttt ttcacaacct
      841 ctttatacat tattcctgat ccaatatttc ttttaatgct gcttcaagag aagttaatgg
      901 ttttcccaat actttttcta aatcatcaga ggaaatagct aactgatcct ttttaatatc
      961 atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg aatccggctt cttttagatt
     1021 ttcgacaaac gctgcatcac ttgcttcaat aatttctaac tttttacctg tcgcacgctc
     1081 taatgccttg gctaattctt catagtcagt tagattgccg gataattcta agatttctgg
     1141 agagtcactt tctactagta cttttgctgc agcttctgca tattcacgtt tcaatgccca
     1201 acctgttttt ccatttcctg cagcataaac gaattgacca gtttttaaag cctgcccaat
     1261 gataggtaat tcattctcta cataccaatt attccgtaaa aatgtatggg aaatacctga
     1321 atccttaatc attttttcag tgaattggtg atctggcgct aatacactga tcgagtgatc
     1381 ggcatctgca aaacttgtat aagcgatata agatacccca gctgcttgtg ctgccttcac
     1441 tacattctta tgttcggctt gacgatttcc tggaacgcca gagacaaaaa gcaatcgatc
     1501 tattcctttc aacgcttctt tcattgagtc taaatcatca taatcaccaa tacgtatatt
     1561 aaatccagct tctttcagtg cttctccttt ttcttcacta cgtactagta catagatttc
     1621 attttgtgga accatctttt ttaagaattc cagcgcataa ccaccaaaac ctccagttgc
     1681 tcctgttact aaataatgca tatttgccac tccctattct ttttatttgt taagatttac
     1741 agctgtacat ttataaatta cacctttatc ataaagagtg tggtaagata agtcaagcaa
     1801 aaaaaatgaa aggatgacaa ctgatgaagt attctctgca atttagtgat gcaatccatg
     1861 tacttgccta tattgaaatg tttcaagata cccgatcact ttctagtgag atgattgcca
     1921 gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat gagtcagctc aaaaaatccg
     1981 gtttgatctt tacaaaaacc ggaaaaccgc agccttccct tgccaaaagt ccagatcaga
     2041 tttctttact agatgtctat cgcagcatcg aaggtaatac acagcttatc caagtcgatc
     2101 caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca agaagcttta gaagaaagct
     2161 acgctcggct gcagaaaaag gcagaagaag aaatggctaa gatcacactt gatagtctga
     2221 tacataaaat agccgttttg gaaaaagaaa aaagaccaga aaacacaggc ctcgtagaga
     2281 gatttttata attctctcta ctgtgcctgc atttttctga tcggaatcgt ctacataaat
     2341 tcttttattc tccgggggac aaaagccttg tcatgcccct ttttcctctc taatgctcag
     2401 gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt caagtagctg accctcggca
     2461 ttagttctga aaaatgcaaa atatggaaag ctatttccca tttttcctct ctaatgctca
     2521 ggtcaaagcg ctactttcac gacctctata ttatcatggt ttattgacta ccggtatcca
     2581 gatttctccc atatatttcc cgttggtaac tcccatttcg actacagcat ttggaccccc
     2641 aacataggcg taatcttcta cttctcttaa aacatgccca aaggccgtat tcgtcaaatc
     2701 atttcttaat tcttcaggcg attctgctaa agcgattaca acaagatacg ttccttttgg
     2761 gaatgggatg attcgggtag tgtttggaac atcctgactc gtttgaacac cgacatagta
     2821 catcattttc ccgttatagg gttcgttgac tgtaaagaaa taatgatttt cagcagcttg
     2881 tttcaatgca tctattgttc cattaccgat aactttttgc tcaaatgctt gtttctctgc
     2941 agccaaaccg acaaaatccg tattgctact cttcaattcc acaccaatac ccgtcaccgt
     3001 aaaatctggt ttttcttcga tcctatactt tgccatttct cccacccttt ctattatatt
     3061 aagttcatct tacataaaat taaagggaaa tgacacgatt acgctctttt ttctagggat
     3121 atttttattt tttaactatt tttttgcttt cactgcaatc caaatctcac tgtaaacatt
     3181 actgaaatcc tctccccatt ttgtaaaaga gatctcggga gcttcgacta actcataatc
     3241 tgaagaagga agccattctg catagatttt cccccaagta tcttgtaaag tttggggaaa
     3301 tggcccttta ttaggaaaaa tggcccaagt gtgttcttct atagcaagca tttctaaatc
     3361 agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg tgcatcaaac tgcccttttc
     3421 ttccaagcgg gctccatcaa atccataaga aacgttgatt acttgatttg gatataaatc
     3481 tcctaactta tgcatctctt ccctttgagt ctccgtgatc gtctgcgcta acttttgtat
     3541 ttcttgattg actccttcaa atttgacagg aacttttttc gccacacctg ccacataaaa
     3601 ctgttctttc ttttcgatac gaaattccat cgattttcct cctctgacat caataaaaaa
     3661 tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg atttctgaag gcagatatcc
     3721 tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt tcatatccat atctaaacgc
     3781 cgtttctgtc actcttgatc catctaggag atcgcgatta gccaaagcca atctacggtt
     3841 tttgatatag tccattagcg acatccctgc aataaacgta aagatgcgtt ttaattgata
     3901 ttcagagatt cctgtatttt cggctattat tttataggaa agtccttctg tcaaatgttc
     3961 ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata tttatcttcc tccttgcttg
     4021 cctttttatc atattatcaa agaaagtcaa ttacttgatg aatcaggaac caatttagtc
     4081 agatgtagaa aatgttgtat ggatctaaag aaaattctca agcagcgttt cgtaacggat
     4141 aattggtatt atctgcctat ctatgtttta cctttagaaa aataaatttt ggggaagaca
     4201 cattctaagt aaacaaagcg gtaaaaatat cttttattcc cttagttaat ccgcttacta
     4261 cctactgtac aaagcaaatt gttgttcaaa ctgctgatca gaacgctgct tggatttttc
     4321 tttgcgatac atcatacctc ttttatcaat tggaacaatt atttatttct aacaaaatca
     4381 cttcattttt gtcagataaa taaaaaacaa aaaaagttcc atctttaaaa cacgctattc
     4441 ttttactttt tctcacattc tctaagaaaa gaaaaggaga atgaattttt gacaattcga
     4501 gcaaaagtga ttatcaaata tgtaaaatca gctacttttt tcttaaacgt ttctttatac
     4561 taaatataaa taagtgtttc atgaaaggag aactagatga ataattggct tgagtatttt
     4621 cctgaaaacg ttttggaacg cggttacagc tatcatctgc acggttttgt ccgtcatttg
     4681 aactatacaa gcaagtactt gtccgcaaca gtttctggca cggaagatta taaggtagtg
     4741 atcacttggg atgaaaagac caacatgact tgtgattgtc tatatgccat agaaggaaaa
     4801 aaatgcaaac acatggctgc tgtcttattc gcttacgaag aacgtccaat aaaaaaatcc
     4861 aactactcac tttccgaact ctcgtccctt gtttcctccg ctagttcctc tcttgtacgg
     4921 gagcttttaa cagagattct tatagaacat ccgcaattta tcgaacgctt caaagttaag
     4981 atgccatttc atgcaataaa ttattcagat aaattaacaa ctatcatcca taaatacgat
     5041 catattatca agaaaaacaa aaacaggaaa acagcgaagt tcatcatgga gatgcgaaag
     5101 ttcatccaag aagcagttga atcgctcatt cagcaaaatg cctatcttcc cgcattcgaa
     5161 ttgatcaatg aagtgattgc tactctcgaa acattctatt gggagcctga ggatgaacga
     5221 acccttcttc tgatagagga ctgttattat ttatggaaag aattattagc agaagcacct
     5281 catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc aagtcgatca tactgacgcc
     5341 tcttattcaa aacgctactc cataaaaatc ctgaaagaag actttcggga aaaagaattt
     5401 tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa cggtaaagaa agacgatttc
     5461 aatgaaaaat agcaagtaaa cgataacaac catcctaaat ccacaagaaa tacagcagac
     5521 cttgctttaa aaacacgaat catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca
     5581 atattagtca tttgactatt aggtacgcgt tttgttttat aagtcatgac aaatttcctt
     5641 cgttgctggg tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg
     5701 taa
//
LOCUS       DANKHJ010000146         5588 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000146 DANKHJ010000000
VERSION     DANKHJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..225
                     /locus_tag="PW113_003027"
     CDS             <1..225
                     /locus_tag="PW113_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL2527266.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            225..572
                     /locus_tag="PW113_003028"
     CDS             225..572
                     /locus_tag="PW113_003028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527267.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            569..1618
                     /locus_tag="PW113_003029"
     CDS             569..1618
                     /locus_tag="PW113_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2527268.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1633..1839
                     /locus_tag="PW113_003030"
     CDS             1633..1839
                     /locus_tag="PW113_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527269.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1860..2456
                     /locus_tag="PW113_003031"
     CDS             1860..2456
                     /locus_tag="PW113_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HDL2527270.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3408..4007
                     /locus_tag="PW113_003032"
     CDS             3408..4007
                     /locus_tag="PW113_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2527271.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4056..4772
                     /locus_tag="PW113_003033"
     CDS             4056..4772
                     /locus_tag="PW113_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL2527272.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4786..5565
                     /locus_tag="PW113_003034"
     CDS             4786..5565
                     /locus_tag="PW113_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL2527273.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
BASE COUNT         2034 a          785 c         1084 g         1685 t
ORIGIN      
        1 aaaacaactg aatttgatat tctacacgct ctaatgtcca cacctaataa agtcttttct
       61 cgtagtgatt tgatagagaa agttaaggga atagaatttg atggactaga taggagcatc
      121 gattctcata taaaaaactt aagacataaa attgagaatg atgctaaatc acctatgtat
      181 attttgacag tccatggtac aggatacaga tttggtggcc actaatgaga aaaagaacga
      241 taaaaactca attagctgtt tcctttttag caatcgcaac attaataata ggatcgatta
      301 gtttagtcgc attaagtttg atgaacaatc attttagtaa atacgtggaa gagcggcaag
      361 aagatttgtt gaatcagtat gtatatacaa ttgatttatt gtggttaaat agtggggaaa
      421 catggaatag tgaagaactt gcagcactat ctgagaaagt attagagaat aatatttatt
      481 tttctataga agacgaacaa ggcaacatgg tttgggaact tactggaaaa gatttgaagt
      541 cagcacagga gaaactgaaa aaaatgcatt gaaagtttct gagaaaaaca gtgtgaaact
      601 agacgaaaca atagaagtga aaaagaagtt aattaatgat ggaaacgagt ttggaaaagt
      661 aacgttttat tatttcggac cttttgctta tacagagcac gatgccttgt ttatctccag
      721 tatgaaacaa agtttaatgt atgttgcaat tgcagctctt cttgtatcat tcatacttgc
      781 ttcgtggatc tctgctagat taggattgcc actaaagcat gtaagcgatt ttacacataa
      841 attaactcgt ggggaatatg ctgataaaat cccacaagaa acttcaatta ttgaaataaa
      901 ttccttaatt gattccctaa atgatttaag taatcagttg gagaaacagc atgggcttcg
      961 aaaaagatta actaccgata tttcacatga attaagaact ccattagcta cgcttaaagg
     1021 caatgtggaa ggaatgattg acggagtctg gaaaataact cccgagcgcc tacaatcttg
     1081 ttatgacgaa atagatcgtc taactcgttt aattggaaat atagaaataa tcaacaaaat
     1141 tgaagcaaaa tatgatcatt taaataaaac tgagtttaac atatataagt tgattgaatc
     1201 cgttattgaa aatttcgcca gcaaaattga gtcaaaaaac cttcatgttg aaatccaagg
     1261 ggataacatc aatatatctg cagataaaga taaaatgaac caagttgtta tcaatctttt
     1321 gaataatgct attaaattta cgcaaaagga gggaaccatt aagttttcta taagtaaaaa
     1381 taaagaccat gttcttttaa ttgttgaaga caacggcatc ggtatagaaa aagatcagca
     1441 acttcatatt tttgatagat tttatatggc tgacccctcc agaagtagag ctttaggggg
     1501 gcaaggtatc ggattagcta ttgtaaagag cgttattgaa gcgcataaag gtagtataac
     1561 tgtaaaaagt aaattaggtc taggaactaa atttgttata aagttaccat ttcaataaag
     1621 gaggagagtt atttgtttca aaaacagata aaaattgcta aactagtatg tgaatgttgg
     1681 gcaaaaaaaa tattttttca actgatttcg atagatagtg tcgaaaaagt tcatttagat
     1741 atcttatcaa attctgtagt aatcttttct aagaaggact tatcaatagc tacaattaaa
     1801 aaagaagttt ttggattaca aatttatgag atgacctaaa tgtgattaat taaaggagga
     1861 tgaagacagt gaaaaagatg acaaaattag tatttgcaag tcttggaatt attgtatttt
     1921 taggagcgtg tagcaatcaa agtgaatcga ataattctaa tagcacaaac gaggaatcga
     1981 cttctacagc gtctagtgaa atgaatagta tggagggaat gaatcacgaa ggtatggttc
     2041 cttcttcaat gaaagatgct gcgaacccta aattcccggt gggaagtaat gttatactgc
     2101 taggcgatca tatggaaggg atgaaaggtg caaatgctca agttgtaggg gcctttgata
     2161 ctacaatgta tgaagtaagc tatgagccaa aaactggggg accaatggtt aacaatcatc
     2221 gatgggtagt acaagaagag ttaaaagata ctgagactgt agcaaatgag ggcgatactg
     2281 ttatcttgaa tgccgatcac atggatggga tgatgggagc tgaagcaaaa gtagataaat
     2341 ctattacagg aactgtttac gttgttaatt acactccaac cgatggtcaa gaagaagtta
     2401 agaatcatat gtgggtaact gaagatgaga tggaatatga cgaaaataat gagtaatttt
     2461 atatactaaa ccaacaagct ggtacatggt cttcgtattc tcaacatgag gaatatgagc
     2521 tctttgttaa taaggactga tgagttgtgc aaaatcaaat ctgggtcaga acgaacctca
     2581 ttctggctca gatttttggc tcttcgacat ttagtgctgg tagagagaca actgaaaatt
     2641 tagtcataac cagttaggca gagtgcttaa ctggtttttg ttcgcattta ttattctttt
     2701 gatattggct gcttgccctt caattttatt tggtttctta atcaactctt catataaccc
     2761 caaatcatat attaatgagg tgcctcctat tcctataaat ctatctcttc tttaatagta
     2821 tgagaaaagt ctactcttgt agatttaaat tgtcaacaaa aaatttacag atgtaaatga
     2881 taagttgaat agagaaagat aaattatttt tttggtattc atataaggtt aattatgtat
     2941 tatcacaaaa tgattgttgt cgaataacta aaagtgtcaa aacaatgatg aaatcattta
     3001 gttcttataa attagacaag agaaaataaa tattccatat ctactttatt ggatccataa
     3061 catgactaaa ttctgaatac tttttaaagc ttatcatttg gtaaaactga atataagtta
     3121 attatataga ctgaaaattt tgaattttca gaatagaata acttaaaagt actgacaaat
     3181 tttttaaagg ggcaatatta atgttctgag ataatcaaat cttaccgaaa atatcaaaag
     3241 tccaatgtga cacaattgga ttttgtcaca ttcgttaaaa tcgctccatc cttagaggct
     3301 ctaataggaa atcacttttc ttttagcgcc ttttttctat agcttgttta aatgtgactt
     3361 attatctgtt ataatcgtat taagtaacat ttgagaggtg tctaaaaatg aaaagcgtgg
     3421 aaccgattcg cgacaaaaaa aagatcgatg ccatgaaggc cattttggct tccggaaaat
     3481 atgggcaacg aaatttggtg cttttttcga tcgggatcaa tacagcctat cgaatctccg
     3541 atttaagaca gttaaaatta agggatgtgc tagaaatttc ccgtgggcgg gtgatcgtca
     3601 aagaacgtct ggccatgaag gaacaaaaaa cggccaaaca caattcggtc ttcatttcga
     3661 acaaattgcg aaaagtgatt cttgattatg ttcaatccga atttcctgag cagttgcaag
     3721 cacaagactt cagcaagtat ttgtttccaa gtcgaaaagg agcagatacg cctttgaccc
     3781 gacaaagcct ttggcggatc attcatgaag caggaactgc agttgggtta aaagaaattg
     3841 gtccccattc gatgcgtaag acctttggct attttttata caagcaagga accaaaacag
     3901 agatcattca gtcgctgcta aaccattctt cacaacggga gaccttacgc tatattggaa
     3961 tcacccagga agacaaagat actgcagtaa agagtttgga tttatgacag aataatgccc
     4021 tatataatag aaggaaaaat tcaagaggtg agaaaatgaa cgaagaaaag cagcctaact
     4081 ttcctgataa atatcactta tctcgcaaag agtctgtgta tttattgaag aaaaacattg
     4141 tagaactggt gtataatgcg gggaaatttg aagggttaaa tacaacgttg ttacaaacag
     4201 aagggattat taagtataat cgggccaaca acgtggctgt agatgatgtc ctgaccgtcg
     4261 ttaacttaaa aagaggcttt gaaatgcttt taaatgacgt acaggagccc ctgttagaga
     4321 caagtaagcg gattaatcgg atcgttgcag cagaggatgc gctttttccc ggtgaaatac
     4381 gaaccggtgg ggtagaatta tcgacgattc aaggaagata tgtgccacca atgttaactg
     4441 aagacgaggt aaacaatcaa tatggtgaga taatgaatca agagatatca gatacggaaa
     4501 aagccctgcg actgtttcta ttcatttcga aaaatcaaat attctggaat ggtaatgtgg
     4561 gtacagcctt gttgattgcg aataagatta tgtttagcaa cggtttagga ctattatcaa
     4621 tacccgaaaa tgtatttatg aaatttaacg aacttttatc aaagtattgc aacagtaatc
     4681 tattagtcaa tgagtcaaat attatatcat ttttatatga acagtgtatc tttggtatta
     4741 attatttgaa aataatccaa catacaattt aagaggtata agtaaatgat gactatagac
     4801 gaagcaaaac aattagcaac ttatttttca gttggttcag cgggatatat tttagacttt
     4861 actaatgtaa cgtggaacat atttacaaag aaaatcgtag ggattgaaat acaaaatcat
     4921 tatgggggct caaagggagt atcctttcaa aagtttctgc ttgacgatca atttgatgat
     4981 tcgttaaaga atcggttaat ccaatcttta aaaggtatta tggatcgaga taaaaaatat
     5041 gaatctactg aaatgaatcc catagttcag ttgctttttg aggaagtatt taaagaagat
     5101 acttcaactg cattaagaaa aatgaaattt tcaagcgaga ttgatttact ttatattcgg
     5161 cagctaccca ttaggattca aacagatatt aaaaacaagg attttgactc tgttttgaca
     5221 aaagcaaata ctttaataga tgaagttcta aaatttatta ttgaaaatac cgaaggaccg
     5281 agtgttaata ttaatttatt taagtcaaaa gaattaagaa aagaagtatt tggccgcctc
     5341 aatattaaaa ctgaaaaaga tatggataat agaatcaaag cccttgttgg agcactcaat
     5401 acattatcag ataaaatact cgaaatgaga aatagtcaag gagatgctca tgcacatggc
     5461 tcaaatagaa tagtaattaa ggaagaagaa gcaattcttg ttgcaaattc ttctatggtt
     5521 ttgtgcgagt atgtgtttac taagtataaa ggattaaata gataataaaa tgcatatttt
     5581 atagtata
//
LOCUS       DANKHJ010000147         5549 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000147 DANKHJ010000000
VERSION     DANKHJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..131)
                     /locus_tag="PW113_003035"
     CDS             complement(<1..131)
                     /locus_tag="PW113_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527274.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDK"
     gene            complement(148..2382)
                     /gene="pcrA"
                     /locus_tag="PW113_003036"
     CDS             complement(148..2382)
                     /gene="pcrA"
                     /locus_tag="PW113_003036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2527275.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2537..4096
                     /locus_tag="PW113_003037"
     CDS             2537..4096
                     /locus_tag="PW113_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2527276.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(4207..>5549)
                     /locus_tag="PW113_003038"
     CDS             complement(4207..>5549)
                     /locus_tag="PW113_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2527277.1"
                     /translation="KKKAKEMGVEIKVETNGSEGIKNRLTAEDIARAEGVIVAADKKV
                     EMNRFDGKELINRPVSDGIRKPEELINLALSGTAPVFHGDGKESSSEEGNADGTIGQR
                     IYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGVPHDQLSNLGNYNQAASWFNQIGQ
                     AAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALANTGGAGFLGALIGGFLAGYVIVFL
                     RKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLVNVPMKAINDALNGFLTSMSGSNA
                     ALLGALLAGMMAADLGGPINKAAYVFGTATLATTVATGGSVVMASVMAGGMVPPLAIF
                     VATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIPFAAADPIRAIPSFIIGSALTGGL
                     VGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGAVVSGVIYGLLRKAPAAAEA"
BASE COUNT         1469 a         1200 c         1063 g         1817 t
ORIGIN      
        1 tctttatcat aaacatagtc ttccactgtt ggtttatctt ctacgtagta ttcgcgagac
       61 cattggttca gtcttgtacg aagttcttct gctcgtttag cagcttcgtc aatcgtcatt
      121 tcatgttcca tcatttaccc tccactacta agctttttca attggcgcaa aggcagccaa
      181 aagacgtttt actccttgac ttgggaaagc cacatctagt tctagatcct tcgacgtccc
      241 gcttactcgg acgactgttc ctagacccca ttttttgtgt ttgactttat ctccaggttt
      301 ccagctgtta ttttcccctc cagtagcact tttagaggga acaaccgctg tctctggctg
      361 tttatagcta tatgctgttt ttttagcgaa tacaggtgtt tttttaggcg taggacgcat
      421 gccttcgatt tccagtaact cctgatcgat ttcttctaca aaacgagaag gtcggttata
      481 ttgcgtccgt ccatataatg ttctggagaa agcatttgtt aaatacaacg cttcttctgc
      541 tcgcgtgatc cccacatatg cgagacggcg ttcttcttcc agttcgcttt cttccatcaa
      601 tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc aggaatacaa ctgggaattc
      661 taatcctttg gctgcatgca aagtcatcaa tgtcacttgg gaagcatctt cttctaaatt
      721 gtcaatatcg gaaacaagtg ccagatcatt taagaagacc gttagttttt cttctggtgc
      781 atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg aattcttgtg tgaccgttaa
      841 aaattcctcc aagttttcga tgcgtgcttg tgcttctaat gtattttgga tttttaagtc
      901 ttctagatat ccgctacgat ccaaaacctc tttcgtcaat tctgtcactg tcaagtatgg
      961 gatcatctga gtgacttctt ggatcatctc tccaaatgca cctaattgct gtcctgcttt
     1021 tccaccgata tttgccagat ccacattttg cgctgcttca agcagtggcc attcatgaag
     1081 agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg atgcctcgtt tgggcgaatt
     1141 aacgatacgc tcaaaactga ttgaatcttg aggatttgcc agcacattca aatacgctaa
     1201 aatatctttg atttctttac gatcgtagaa cttgtgccct ccaaccattt tataaggaat
     1261 atttgctttc aacagagttt cttccattac acgagattgt gcgttcgtcc ggtacaagat
     1321 ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct tcttgcatcc gatctacgat
     1381 aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa gtgatcttgt ttccttcttt
     1441 gttttctgtc cacaagtttt ttggttttcg attgctattg ttttcaatga cttggtttgc
     1501 tgcgctaagg atgtttttcg tggagcgata gttttgttct agcaaaatta ctgcagcatc
     1561 tggataatct ttttcaaaat ccaagatgtt ctgcatatct gcaccgcgcc agccgtaaat
     1621 actttgatct gcatctccca cgacgcacag gttcctaaat ctacctgcca ataaattgac
     1681 tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct acatgaatat aatggaattt
     1741 gttttgataa tatgtgagtg aatcaggatg ttcttcaaat aatcgaatcg tattcatgat
     1801 caggtcgtca aagtccatac attgattata ttgaagctct ttttggtaag cagcataaca
     1861 ttttgccgca atctcttcgt aataactgcc ttgcattttt gcaaaatcct gaggggtctg
     1921 caggctgttt tttgcttgag agattgttcc taatatactt cgaggatcat attttttcgg
     1981 atcaatgttc agttctttta gtatccgttt catcaaggtc agctgttcag aagaatcgat
     2041 gattgtaaag ttccggttat acccgataaa atcaacatct cttcttaaaa tacgcacaca
     2101 catcgagtga aaagtcgata cccagacatc ttcgcctcca gatgcgagaa tcgcattgac
     2161 acgttctttc atttcttttg cagccttatt ggtaaaagta atcgctagga tgttccaagg
     2221 attgacttct ttttcctcaa tcaaataggc aatgcggtgt gtcagcactc tcgtctttcc
     2281 acttcctgca cctgccatta gcagtaatgg tccgtcagtg tgaagaacag cttctttctg
     2341 acgggggttc atcccgttta ataattcagc tttggatgac attaacaaca tcctttcttt
     2401 tatcaatctt gttaattata gcattttctt ggctttcatt aaactagcta gttcaaaatc
     2461 atttgtttct ttttatggat ttttccttgt caattcgttg atttcgcgct aaattaaaag
     2521 aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag aaattagata agactcagat
     2581 atattttatt ctaaaaggaa tatttgttgg actgctgaca ggaatcgtcg ttagcctgtt
     2641 tcgattatct atcgaaaagc taagcgattt agttcgaagt atctatgaaa tgagtcggga
     2701 acagccgatt tacttaattg gcctagctat ttgctgcatc cttgcagcat ttttcgtggg
     2761 ttatctggtc aaaaatgaac cggatatcaa gggaagcggg attccacaag tagagggaca
     2821 gcttcgcggg gaattatcaa tgaactggtt ctctgtttta tggaaaaagt tcattggcgg
     2881 tgtactttca gttggtgcgg gcctttttct aggtagagaa ggaccttcta tacagcttgg
     2941 cgcatccatt ggacaaggag ctggacaatt gtttcggagt ccatcttcag aagaaaagat
     3001 tttaatttct agcggtgcaa gtagcggact tgcagcagca ttcaatgctc ctatcgcagg
     3061 attactcttt gttttagaag aaatccatca ctccttttct ccacttgttt ggctgacatc
     3121 atttgcttca gcaatcactg ctaacttcgt ctctctttac tttttcggct tgagacctgt
     3181 tttatatcta ggtgaccttc cttctttgcc gttgaaatat tatggttcct tagtgatctt
     3241 aggagtactt ctaggcatct taggttttat ttatcagaag gtcttgctgg ctttgccaaa
     3301 attttacaac cgattgcctt taccagctta ttttcacggg ttcgttcctt tcttgctgat
     3361 tttgccaatt ggttatttat ggcctagtac tctaggcgga ggcaatcaaa tcgttttagc
     3421 ttttggacat gcttccttgc caatcattat gattatcggt ttgtttgttc ttcgattcgt
     3481 tttttccatg atttcttacg gtggtggtct tccagggggg attttccttc cgatattgac
     3541 attgggggct ttaatcggta gcttttatgg aaacattatg gttgatttcg ggatggatcc
     3601 aatctacatc aaggattttg tcgttattgc catggctggt tatttcacag cgattggaaa
     3661 agcaccgctg acagcaatta ttttagtgac agaaatggtt ggctctctta atcatctgat
     3721 gcctttagga ttggctgctc ttgttgctta tatcgtcaat gaccttctcg gcggtaatcc
     3781 aatttacgaa tcattattgg aacgtctact tggaaatact cagaccgcct cactaaaagg
     3841 tgcaaggaca acttttgatt ttccagtaac ggcagagagt tctttagacg gattaatggt
     3901 acgagacttc aattggccta aaaatatgct gctaatctct atacgcagag gaaatcagga
     3961 aatcttgact cacggtgata cgatcatgaa tgtaggagat attttggtta tcttgactga
     4021 tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct aaatcttcta tcacaaattt
     4081 gaagcaatca ttttaacgaa catggaaaaa ctattttaca acaactattc atctacacta
     4141 cttgattcta tatgattcta taaaaaaaga agagactggg aaaaaccagc ctcttctttt
     4201 ttatttctat gcttctgctg ctgcaggagc ttttctcaat aaaccataaa tcacaccaga
     4261 tacgactgct ccgatagcaa taaacaccaa atacatgacc gggtgactca gtaagaatac
     4321 aacgaaaatc ccgccatgag gagccagtaa tttgatcccg aaagctccga ctaatccgcc
     4381 agttaaagca gaaccaataa taaaacttgg aatggcacga atcgggtcag ctgctgcaaa
     4441 tggaatcgat ccttccgtga caaacgatag tcccatcacg atgttcgtca aaccagcatc
     4501 ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta gcaacgaaaa ttgccaatgg
     4561 aggtaccatt cctccagcca taacagaagc catcacgaca cttccgccag ttgcaacagt
     4621 cgtagctaaa gttgctgtac caaacacata agcggctttg ttgattggac cgcctaaatc
     4681 tgccgccatc attccagcta ataatgcacc aagtaaagca gcattcgaac cactcatact
     4741 tgtcaagaaa ccgttcaatg catcattgat tgctttcata gggacattga ccagcaacat
     4801 caagaaacca gtaatcagta agccaaacac tggataaaag aggatcgttt taattccatc
     4861 caatgatttt ggtaagttct tgaataattt acgtaaaaat acgatgacat aaccagctaa
     4921 gaatccacca atcagtgcac ctaagaatcc tgcaccgcct gtattagcta atgcaccagc
     4981 agcaaatcca acgatcaaac cagggcggtc accgatactt gaagcaataa atcctgctaa
     5041 taccggcagc atgaaaccaa atgcagcttg tccgatttgg ttgaaccagc tagcagcttg
     5101 attgtaattc cctagatttg ataattgatc atgtggcacg ccaataaatt ggtcgatcat
     5161 gaaagaaagc gcaatagcga ttcctccacc aataacgaaa ggtaacatgt gtgatacgcc
     5221 gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca tccgcatttc cttcttctga
     5281 actgctttct tttccatcac catggaatac tggtgcagta ccactaaggg ctagattgat
     5341 cagctcttct ggtttacgga taccatcact gacaggacga ttgatcagtt cttttccgtc
     5401 aaagcgattc atctctactt ttttgtctgc tgcaacgatt acaccttctg cacgggcgat
     5461 gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg ttcgtttcga ctttgatctc
     5521 cacacccatt tctttcgctt ttttcttca
//
LOCUS       DANKHJ010000148         5461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000148 DANKHJ010000000
VERSION     DANKHJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(110..1252)
                     /locus_tag="PW113_003039"
     CDS             complement(110..1252)
                     /locus_tag="PW113_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527278.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(1303..2100)
                     /locus_tag="PW113_003040"
     CDS             complement(1303..2100)
                     /locus_tag="PW113_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527279.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            2302..2601
                     /locus_tag="PW113_003041"
     CDS             2302..2601
                     /locus_tag="PW113_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527280.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            2866..3741
                     /locus_tag="PW113_003042"
     CDS             2866..3741
                     /locus_tag="PW113_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527281.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            3738..3956
                     /locus_tag="PW113_003043"
     CDS             3738..3956
                     /locus_tag="PW113_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527282.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            3943..>5461
                     /locus_tag="PW113_003044"
     CDS             3943..>5461
                     /locus_tag="PW113_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527283.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERA"
BASE COUNT         1838 a          734 c          997 g         1892 t
ORIGIN      
        1 tctgtaggtt aataactatt tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt
       61 tttttatttg tattttaatg gttggctacg tttttggcta cgtttggaat tattcgccca
      121 taaattttgc aaatcggtca ccagttttct taacagattg tggtgtgaca tgagcataga
      181 tattcatggt agttttaata tctttatgac ctaatcgttc ctgtacttct tttatcgacg
      241 caccactttc aaacaataag ctacagtggg tatgcctaaa tccatgtgga gtaatttgag
      301 gctccatcgg tgtctttttg taaatccagt ctaaccaatc gtttactact tgggggtagt
      361 acaacttgtt tgtgtttgta gtgaaaagaa actgttttgg tgaattcgtg ttatagccat
      421 ataagatcat tattttcatt tgttcttgtt gccaaatacg cagttgttta agtgtaaaat
      481 catcaagctg aatcgttctt gatgaatttt tagttttagg tacttgtaga actacttgat
      541 taaactcgtc ttgagcgata gttttaccaa ttgttattgt tttattgaaa tagtctatat
      601 cttcccattg aagcgccaat gcttcgcttt tacgcattcc tgtataagct aataaacgga
      661 aaaaagtgta cagtttaact gaattatttt ctttagtatt ctgtaaaaac agttgtaatt
      721 gatctttcgt gtaaaacttc aagggttgtt cttcttcaat ttttcttgga agcaatgttt
      781 tagacatagg attatcctta cagacttcca ttgctactcc aaatttaaat atttgagcag
      841 ttgtttttct caagaaacca tattgtttta atgttttgtg ccattcattc acctgttttt
      901 ggcaatagct aacacttatt ttatccaatc gtaattcccc aaaagcaggt aatatatggt
      961 ttttgcaaaa acgttgagca acggcaacag aagacggttt tactcttgtt ctgtagttat
     1021 caatccataa ttcatacaac tcttgaaaag ttgtaacatt gattttaaac tcttgttttt
     1081 ctttaaagtc tacaagtagt ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc
     1141 ttctacgctt tgtacgtata tcttttccag tcttgggatc tttccctaaa taagtttcaa
     1201 atccccaata tttttcgcca ttttgtaatt tataactaaa aaatgttgcc atttgattct
     1261 ctccttactt cattattgca ggtagagaag ttatttgaag aatcaattat ttttaaattt
     1321 atcgtgatta tcagttaaaa attttgcgat tatactattt ttctcatatg ggaaattgta
     1381 ttttttacta agatctgaaa taaatttttc tgtttcaaat tggtttttag taattttata
     1441 aaagaaatcc tcgacagatg aatactttga aatatcttta taggctaagt ccatttcgtc
     1501 taatagctgt tttaaagaat caatcattct ttctttttca ccgtatttga aatcaccatt
     1561 tcttatcttg tcttgaagtt tactgttggc tcttttgaaa aatagctctt tagagataga
     1621 gtgtctttgg ttagcgtact cttcaatcag aggattgata ttattagcaa gtatctcatt
     1681 tagataaatt tcaaaatctt ttgagtaata ttttgtaaat atatttttta ttataggatt
     1741 caataactca tagtctttat ctagcgaaaa cgtatcacta tatctttctt gaatgtcttt
     1801 aaagactttg tgaggtattt ctgggaagtc cttaatatat agctcatacc ctatatcgat
     1861 aaggtatgaa gttatatatt cttctaaagt tccccattta atccagtcaa ttgttgaatt
     1921 agtgatagtt gataataatt tcagcttttg tggatttggt aaattggtcc cacgttccca
     1981 gttggatatg gtcgcacgtg gtgaattatc tattttcctt ccaagttctt ccatagtaaa
     2041 accacatgaa aggcgtaatt gtttgattcg ttttcctgct tctgaaggat ttggcttcat
     2101 cttttttctc ctttttattt ttaaagatag tatatcatat ttataaaata aaagtattta
     2161 aaagtattta ctttttatct ggagtatgat actttattct tataaataca aataaataca
     2221 tttttttacg caatttgtat ttatttgtac ttataaaggt agaggagtta tagaataatt
     2281 aattaaaaag gagaggtttt tgtggaagca ataaaattaa tgatttcaga agaacaggag
     2341 gaaatgtttc taaagcagtt ttatcaaatt gctacaaaag ctattgaaaa ggctcaagaa
     2401 aatgcgggga tgacaaggga atatctcaat caaaaagaaa tggcggaata cttaaatgtg
     2461 agtgaaaact ttttaaaaaa tgctgtgaga aatgagggat tgcccgtagt tgaattaggg
     2521 gcaagaaaat tttattcgaa aaaggcagtt aatgagttta tgtttgctcg aatgaaaaga
     2581 ggggaaaata ttggaagatg atgaagtaga aattataaaa aaatgcttta aaaaaagaaa
     2641 aaaagtgttc ccaaaagttt gcagactggg ggaacactca ataataaagt gctgagaaaa
     2701 ctttatttat agctattata tcaaaaaaga aggtgaaaag gtgttttctt ctggaaatgt
     2761 tgtagttgac aaacttagag aaataaattt tgacggaaat gtaatccaca cacatggtac
     2821 gatcaattaa gaaaaaaaac gaaaaaaggt gttgagaagc cctatttgaa tgcaatagtt
     2881 atcttagcag aaattaccta ttggtataga cctaaaaaaa tttttaacga tgatggtcag
     2941 ctacttggtt atcaaaaaaa gtttcttgaa gatattttgc aaaaatcata taagcaatta
     3001 agcaaaaaaa caggattatc agaacgagct ataaaagatg caattgtgta cttagagaaa
     3061 aaggggatta tcaaaagggt ttttagaaat atttattatg agaaaagagc agttacatta
     3121 tcaaatgtta tgtacattgt tttaaatgta cataaattga tagaaattac ttttcctact
     3181 ttggattgcg aaaaaaagga gtatatgccg aaaattcgtc agaggggtat cgaaaaaaag
     3241 agatatgtct ctttaaaaaa aggtagaggt atgtacaaat ttgtatatac gtatacaaag
     3301 actacttcac agaacatatc aaagactact ctcgttatgg aagaagagga tattaatata
     3361 aagaggattt acagtaagat tagtgttctt tatgacgcaa ataacgaaga tggtcgaaaa
     3421 ttatttcaag tgtatcacta tttgagaaac gctaatgtag atacagaaga tatcagaaaa
     3481 atcattgaat atttatctga aaatactcag tatcttattg ctgagtgtat tataaaacaa
     3541 catgaagcat gcttttttca aagaaaaact gaagaagggt tatacgatta tgtaacatat
     3601 tttttgaatg gacttcagaa aagatataaa agtcaatttt tagaaaatgt agagcctata
     3661 gatcagttca aaaaagcttt cgaaatcaaa gagttacctg aaattactat gcataattgg
     3721 ttgaaaggag aagaatcatg attaaaaatc gcttaataga tcaaatgtgg tgtcttgatc
     3781 aaaatacaga tactctcgaa ttaatcgaga gtatctgctt caacacagta gttctcgatt
     3841 taagagctga aaatgacaat tgtgtaactc atatcataat taatcaaaaa atggctcagc
     3901 agctctctga gagcctaaga aaatgggctg aaggagaaaa ctatgaaagc aattaaagaa
     3961 aaaatcgtta gtagaaaaat aaactggtta gcactcagtg tacttgtttt aatgattagc
     4021 gttgttttta caagtaatat tgttgaagct agcattttca attggtttga tgacacaaaa
     4081 agtcaaacag agtttttgaa taattctctt tatagacctt acctaaaaaa atacaatgga
     4141 gatttcctta gtcagttcgg gatttggctt ggatgggctg tggtaaaagg catgtttact
     4201 gtgactgatt cgattcaaaa tatgattcca gatgtgttgg atttgttcaa tttcattgaa
     4261 agtacagggc taaataatgt ttatcaatca gtcataaata caattgtcgt tggattaatg
     4321 atcctttcat tgatgtttgt tggctacaag atgattactg gaaaaggaac aatagattta
     4381 agatctgttg gaatgaatat agtgatgtct gtagcgttaa ttcttttaat gccaacaatg
     4441 atttctagcg gtattcaatt tgccaaaata ttttacaatg atgctactac gattacaaat
     4501 tcagatgacg gagtcgcttg gtcattgata aaacaaggag taacagatct ggcatatatt
     4561 aataaaacag atcaatactc aagcattgat aaaacggagg atcgaaataa gttaacacga
     4621 aaaaattttc aacagacaga tttaacacaa gtgttgacgg ataaagtgat tgataaattg
     4681 gaaaaagaaa atccagctgc ggataatctt cgctatgaat tagttgaaaa ttctaataat
     4741 gaatttgttg ccacaaagtt ttctgataac ttcttatcaa cattttctga tagcttgaaa
     4801 tcaggttatt atcgatatca agcaaatctt tgggggattt cgattgggtt gacagcgttg
     4861 gcaatcgcct atgtgttcag cgcgtttgtg atcattacag caatattaga acttgcattt
     4921 aaacgtgtgt taggtgttct tgtttttgcg acagatattg aaacaggaca acgttcaaaa
     4981 gttgttttat ccgatatttt acaatgttac ttaacagttg gatttcaagg gtttggacta
     5041 tctatgtttg ctatgtttat caatttttta aatgctggac aaggtatctc cacaaatatt
     5101 tttataaaaa caattgctta tatctgtgct gtgtttgtgc tgataaaagg cagtggaaca
     5161 gtcatgagat actttggtgt tgatattgga ctcaaagaag gctatgggca acttgcttct
     5221 gcttttggta tgggcgcaat gctgtttaga aaaggatcaa ctggatttaa tcgtgctaaa
     5281 ggaagcggaa atggaaatgg atcagacagt ggagaaggag aagatcgaaa accagaaaag
     5341 aatttcggcg aaactctatc aaaaaaagct ggcaaaactg gaagagcttt gggatatgcc
     5401 catgaacgtg gattatcagg tttagcttca gatggagcta caatggctag tgaaagagca
     5461 a
//
LOCUS       DANKHJ010000149         5339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000149 DANKHJ010000000
VERSION     DANKHJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(19..414)
                     /locus_tag="PW113_003045"
     CDS             complement(19..414)
                     /locus_tag="PW113_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527284.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="PW113_003046"
     CDS             complement(404..616)
                     /locus_tag="PW113_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527285.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="PW113_003047"
     CDS             1688..2290
                     /locus_tag="PW113_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527286.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="PW113_003048"
     CDS             2645..2881
                     /locus_tag="PW113_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2527287.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="PW113_003049"
     CDS             2878..3291
                     /locus_tag="PW113_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2527288.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3810..4760
                     /locus_tag="PW113_003050"
     CDS             3810..4760
                     /locus_tag="PW113_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2527289.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="PW113_003051"
     CDS             complement(4757..>5339)
                     /locus_tag="PW113_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2527290.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DANKHJ010000150         5312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000150 DANKHJ010000000
VERSION     DANKHJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW113_003052"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PW113_003052"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2527291.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PW113_003053"
     CDS             complement(1297..2133)
                     /locus_tag="PW113_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527292.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PW113_003054"
     CDS             complement(2317..3534)
                     /locus_tag="PW113_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2527293.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PW113_003055"
     CDS             complement(3683..3862)
                     /locus_tag="PW113_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527294.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PW113_003056"
     tRNA            3917..4000
                     /locus_tag="PW113_003056"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="PW113_003057"
     CDS             complement(4240..4818)
                     /locus_tag="PW113_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527295.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="PW113_003058"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="PW113_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DANKHJ010000151         5219 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000151 DANKHJ010000000
VERSION     DANKHJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..439)
                     /locus_tag="PW113_003059"
     CDS             complement(<1..439)
                     /locus_tag="PW113_003059"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2527296.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARI"
     gene            complement(430..2076)
                     /locus_tag="PW113_003060"
     CDS             complement(430..2076)
                     /locus_tag="PW113_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2527297.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2260..2922
                     /locus_tag="PW113_003061"
     CDS             2260..2922
                     /locus_tag="PW113_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2527298.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3066..3629
                     /gene="ahpC"
                     /locus_tag="PW113_003062"
     CDS             3066..3629
                     /gene="ahpC"
                     /locus_tag="PW113_003062"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2527299.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            3639..5168
                     /gene="ahpF"
                     /locus_tag="PW113_003063"
     CDS             3639..5168
                     /gene="ahpF"
                     /locus_tag="PW113_003063"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2527300.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT         1631 a          980 c         1009 g         1599 t
ORIGIN      
        1 cgatgcgcgc gacgatttct cttggatcat ctggaatatc atcagatata aaagctaacg
       61 tggatgagcc gtcttctaag cggtagtcaa ggtcagattt cacccagtca aatacaggca
      121 taaaattata gcagatgact tctattccat attctgccaa gtttcggatt gtctgcttat
      181 agttttcgat atactgctca cgggaaggta atccaatttt aatggcatca tgcacattga
      241 cgctttcaat tacttttagt gttaacccac gagactcaat ttcttctttt agttctttga
      301 tcctctccct aggccatacc tctcctactg gtatatccat caatgtgcct acaatccctt
      361 tgaccgcagg aatctgtctg atttcatcta aggtaatctt ttcttcctgt gatccgtacc
      421 atctaaaagt catttccatg ataaatttcc tccatgttct tgtaagtaat cctgtaatgt
      481 ttttcgtacg gcatttggtc ctgctaccat cttgatgaac atttcttcta tttttctgcc
      541 aagctctgct tggtaaagat ctacaccaaa aatttcttta tttgacaaaa taggagccat
      601 tttctcatga atatgtgatg tatcttgcgt acccagttga atatcggaaa gcttatgctg
      661 aaggtctgtc aatagtgggt ctggactcgg gatgaatggt tttccttgat catcaatgcc
      721 aataaggtat cgaagccaag cagcgagagt caatggtatg aatacaagtg tctgcgggtc
      781 tttaacttct atttccatat attttttaat cgtctcacca taacgaatcg ccaccttttg
      841 agaggtatcc gtggcgattc gctgaggcat attaggaata tttggattag aaaagcgcac
      901 agtaattact tcttccagaa agatctttgg atcaatgata cctggagact ccacaactgg
      961 gaggccttca ttgtacccga tttcagaaac tagcagcgat aaatctggat ctttcatctc
     1021 ctcagctatg ctcctatacc ccagtaaaca acccataata gctaaagacg tatgcaaagg
     1081 attaaggcaa gtcgtcacct tcatcgtatc tgctttatct accgttgcac gatcagtcaa
     1141 gaatacccca gctttttcga atttcggacg tccattggga aaatgatctt ccatgattaa
     1201 ataatggatg tcctctgtat tgacaaatgg agcaatatta tttccttttg cggtttggaa
     1261 aatcgacata tttgaaaagc cctcttttgc caacattttg ctaactgaca aggaaggatt
     1321 cggggtgata cgatcaatca tcgtccatgg aaacgcaaca atttcttcat tcgataagta
     1381 ttggataaat gtattttgta cataaccttc cttttgccat tcttttgcga tcgttagcaa
     1441 agaagtacgt aattgactac cgttttgtga aaaattatct gtagagacaa gtgcaatcgg
     1501 ccaagctcca gataaaaaac gtttataaag tagcacagca agaatactca ttgtatgtga
     1561 tggcttcttt ggaccatttt ttatatcgac tgcaatcggt cctatatatt tattatttgt
     1621 atctttcaat cgataacctt tttcagtaat agcaaaagta actaattgta aactaggatt
     1681 ttcaaagtgt cgaatcatct tatcaaagtg ttcagaacaa tctggatgac aatacagact
     1741 gtcagctgtc gcagctaata tacgtttgac gaactctcca ctgcttggca tgaccacttc
     1801 aagaatcaaa tcatcaaaat cttggtaagc cctttcaatt atttcttcat cgaatgtttc
     1861 acaaacaatg acaccagtgg acaattcgcc tagatccgct aaacgttgag cgatttctgc
     1921 atgaaatcct cgataaagat ttccgccacc gaaatggatc cagacaggat ttttttgtcc
     1981 attttctctt aatgatgcac ggtcaaaatg aggtatttct acttcttttt gagataattc
     2041 agggataaag ttcctccagt tgtttaaatc aatcattttt cccctcctaa actaacatat
     2101 tagtttttgt attcaagtga attttatgca ataaaaatcg ctatgtcaac caaaatttta
     2161 ctatttatac aaatttacta gttaatgtga cataccagat tgtttttcag acctcttctg
     2221 ttatactacg ttcatagaaa tgattttgga ggaataaaga tgaaaagcag cttgcaatca
     2281 caagcttatc aagcgattcg taaacaaatt atctatgctg acttagaacc tggaaggaaa
     2341 atatctgaaa aggcactgga ggaacatctt gctatcggcc ggactcccat tcgcgaagca
     2401 ttgattcaac tacgtcagca agaactcgtg tatacgatac ctcaaagtgg tacgtatgtt
     2461 tccaaaatta atcttgtttc tgcagaaaat gctcgctttg tccgagaaca gttggaagag
     2521 caaatcatga ttgaatgttg ttccaaattg gacacacaaa cacgaatagt gctagaaacg
     2581 attttagaag aacaagaaaa agcagtcaaa ataaatgacg aacgcgcttt tttccgtgca
     2641 gataatttat ttcatgaagc ttgtttcgaa attgcaggaa gaaaagaagt ttggaactgg
     2701 ttagatgacc ataataccca tttagaacgt ttccgctggt tacgagtgac cattcagggg
     2761 cttccatggg aaacgatcat ggaacaacat tatgatttac tcaaggcttt agttgataaa
     2821 aaaccagaag aagctcgatt tcaaacggga ttgcacttac acatgatgct gaaagaacag
     2881 gaaactgtta ctcaacgtta tcccgagtat ttccagcagt agcattttct tgcgacctct
     2941 tctattttta taattaacct tcgtttttga gtaatcttta ttaaataaca aaatatccat
     3001 tgactttgca actccaacta aattacaata attataaata aataattatt attggaggtt
     3061 ttactatgtc attgattggg aaaaaaatcg aagaatttaa tgcacatgct tatcatcaag
     3121 gagaattttt agaagtcagt gaaaaagatc ttatggggaa ctggagtatt ctttgcttct
     3181 atccagctga ttttactttt gtttgtccta ctgaattaga agacttacaa gaacaataca
     3241 gtacattgaa atctttaggt gttgaagtct tttcttgctc taccgatacc cactttactc
     3301 acaaagcatg gcatgataca tcagatgcta tcggtaaaat cgaatacgtg atgatcggtg
     3361 atccgtctca tcagatttct cgtatttttg atgttttgga tgaagagcaa ggattagctc
     3421 agcgaggaac atttattatt gatccagacg gaatcgttca agctatggaa atcaatgcag
     3481 atggaatcgg tcgtgacgct agtgcactga ttgataagat tcgcgctgct caatatgtac
     3541 gtacccaccc aggtgaagtt tgtccggcaa aatggaaaga aagtggcgaa acattgaaac
     3601 caagttttga tcttgttgga aagatttagg tggtggatat gttagacaaa gaaactaaac
     3661 aaaatctcga acaatattta gcactgattg aatctcctat tgttttttct gttagtttgg
     3721 ataatagtga aaatagtcaa aaactagctg aatttacgaa ggaaatagca gaaatgtctc
     3781 ctaaaatctc ttgggaaaaa agagattcga tgcgcacacc tagtttttct atcaatccag
     3841 ttggcaaaga aagtggaatc gtttttgctg gtatccccct cggtcatgaa ttttcctctt
     3901 ttttactggc tatgttacaa attagtggac gggcaccgaa aatcagtgaa tctttgagtc
     3961 ttaaaatcaa aaaaataaca caaacattgc attttgacag ttatgttagc ttgacttgtc
     4021 ataattgccc cgacgttgta caggcattga atatattagc tgtattaaat ccaaatatta
     4081 ctcatacgat gattgaaggt gggatgttcc aaaaagaaat cgatgataaa aaaatcatgg
     4141 cagtgccaac tgtgttttta aatggagaag aatttagtag cggacgcatg acgatcgaac
     4201 aaattgttga aaaaatcact ggccctttga ttgaagaaga gttgtttgaa aaagaacctt
     4261 atgatgtatt agtgattggc ggtggaccag cagctagtag tgcagctatc tatgcagcac
     4321 gtaaaggaat ccgtacagga cttgtcgctg attctttcgg cggacaagtc gttgaaacac
     4381 tagggattga aaatatgatt ggtacctctt acacagaagg tccaaaattg atgcagcaag
     4441 tagaaagtca tgtgcgtagt taccctatcg acattatgat gaatcaacag gccgtttcct
     4501 taaataaaga gcaacatttt attaatattg gcttagctaa cggtatatca cttaaagcaa
     4561 aaaccgccat catcgcgaca ggtgcgcact ggcgttctat caatgtacca ggcgaaaaag
     4621 agtttaaaaa taaaggaatt gcttattgcc cacactgtga cggcccttta tttaaaggaa
     4681 aagagattgc tgttatcggt ggtggtaatt ctggtgttga agcagcaatc gatttggctg
     4741 ggttagcaaa acatgtatat gttctagaat tcttaccaga attaaaagca gatcaaatct
     4801 tacaggataa actagtctct cttgcgaatg tcactgttat caaacatgca gcgacgaaag
     4861 aaatcagcgg tacgaatcag gtagaatctc tctcctatat cgatcgccgt acgatggaga
     4921 tgcatacact agctgtttca ggagtattca ttctagtcgg cttactccct aatacggatt
     4981 ggctagatgg aacgatcgaa atgaatccaa gaaatgaaat agtgaccgag aaaaatggcg
     5041 caacgaatat ccccggcgtt ttcgcagctg gtgattgtac agatagtcca tacaaacaaa
     5101 tcattgtttc aatgggttca ggagctactg cagctttagg agcattcgat tatctgatgc
     5161 gcacttgagc tatgaagatt gtaaaaagtc agagagcgga aaaaaggatg gtgacaaga
//
LOCUS       DANKHJ010000152         5165 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000152 DANKHJ010000000
VERSION     DANKHJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..450
                     /locus_tag="PW113_003064"
     CDS             <1..450
                     /locus_tag="PW113_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2527301.1"
                     /translation="GFGILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGAT
                     RWQTIYKVVLRAAVPGILTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTS
                     ILTMGIGNTIMGTVENNVLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            450..1334
                     /gene="pstA"
                     /locus_tag="PW113_003065"
     CDS             450..1334
                     /gene="pstA"
                     /locus_tag="PW113_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2527302.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            1349..2155
                     /gene="pstB"
                     /locus_tag="PW113_003066"
     CDS             1349..2155
                     /gene="pstB"
                     /locus_tag="PW113_003066"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2527303.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            2171..2929
                     /locus_tag="PW113_003067"
     CDS             2171..2929
                     /locus_tag="PW113_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2527304.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            2942..3619
                     /gene="phoU"
                     /locus_tag="PW113_003068"
     CDS             2942..3619
                     /gene="phoU"
                     /locus_tag="PW113_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2527305.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            3857..4888
                     /locus_tag="PW113_003069"
     CDS             3857..4888
                     /locus_tag="PW113_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2527306.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            4885..>5165
                     /locus_tag="PW113_003070"
     CDS             4885..>5165
                     /locus_tag="PW113_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2527307.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLL"
BASE COUNT         1592 a          915 c         1127 g         1531 t
ORIGIN      
        1 gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc gactatcaca
       61 acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc ttcattagcg
      121 cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg
      181 attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt agctatccaa
      241 atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc ttctacattg
      301 acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga aaataatgtg
      361 ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat cgtgatccgt
      421 atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat aaaattgcta
      481 caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct ttactacttt
      541 atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa ccttccaaag
      601 cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat ttgcttttga
      661 tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt tcagaatatg
      721 ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg agctcgttgc
      781 cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc cagtatggtt
      841 tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt ttgactagaa
      901 cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga ttggccttag
      961 gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca ccagggattt
     1021 tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca ttgatctata
     1081 cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta agtgtttcaa
     1141 gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg aagatcaata
     1201 cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc gtgttgatta
     1261 ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa
     1321 tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt
     1381 catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac atgtctggta
     1441 tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata agatcactgc
     1501 actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa
     1561 tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg tagatattaa
     1621 tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt ttcaacgtcc
     1681 taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac agcacggcga
     1741 aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg
     1801 ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg ggcaacagca
     1861 acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc tagacgagcc
     1921 ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg taaacttgaa
     1981 agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc gtatcagcga
     2041 ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta acaagatatt
     2101 taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg
     2161 aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt attatggaaa
     2221 gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca ctgcgatgat
     2281 cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga tgaatgacct
     2341 gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca tctatggtcc
     2401 aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc agccaaaccc
     2461 atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag gggaaaaaga
     2521 taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg tttgggatga
     2581 tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt
     2641 atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg aaccaacaag
     2701 tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat taagagagcg
     2761 gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt cagataagac
     2821 ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga tattcatgaa
     2881 tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag gaggaaacgg
     2941 aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt tctacgaaat
     3001 ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca aacacgataa
     3061 aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg aaacacgctt
     3121 ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg atttgcgtat
     3181 gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc atgcggtgtc
     3241 tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga
     3301 gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt
     3361 caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag tcaatgacta
     3421 tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg agacagtcat
     3481 cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag actatgtgac
     3541 aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt taaatacgaa
     3601 ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg aaaagaggtt
     3661 gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa
     3721 aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca agcttttgaa
     3781 caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt tcatcatttc
     3841 atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt ttatttgtgt
     3901 gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg tcatcgagat
     3961 agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga tgaacacgga
     4021 tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa tagaaacagc
     4081 aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat ggcagctagt
     4141 agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg gcgccatgtt
     4201 gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca atatcgtttt
     4261 acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat taaatatatc
     4321 tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg gatggaatag
     4381 aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat tttttatttg
     4441 tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac tgaattatct
     4501 gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag ctggaggaac
     4561 actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc gacaaccaga
     4621 aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg tgattggctc
     4681 gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc ttcttttata
     4741 tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat tggaattttt
     4801 acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg tcctcttttt
     4861 tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg gattttagca
     4921 atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg cttattcatc
     4981 aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc gatactggca
     5041 ttgtcggtca taggattttt attgattgct gtggttatct tattttttat caacggttat
     5101 aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg tttcctacta
     5161 ttgct
//
LOCUS       DANKHJ010000153         5112 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000153 DANKHJ010000000
VERSION     DANKHJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            16..1092
                     /locus_tag="PW113_003071"
     CDS             16..1092
                     /locus_tag="PW113_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HDL2527308.1"
                     /translation="MSFEDREAKILVDAEGHPNDIQLRSRGIGERLIESFMLAANETV
                     AEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGILVRGTKGTITPKDLQHVIEGVE
                     DKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYTHFTSPIRRYPDLIVHRLIRSY
                     SEDKSESNQSSWAAELPEIADHSSKMERRAVETEREVDSMKKAEFMMDKVGEEFDGII
                     SSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHLALVGERTGLTFKIGQKVRVKV
                     VKADPDERAVDFELVSAEEVTALERPKARNGKSGKREGKQAGKRSEKNGKKNKRYSDS
                     KQTFGKKKGKKPFYKDVKKKKKKK"
     gene            1114..1578
                     /gene="smpB"
                     /locus_tag="PW113_003072"
     CDS             1114..1578
                     /gene="smpB"
                     /locus_tag="PW113_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2527309.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            1880..2599
                     /gene="atpB"
                     /locus_tag="PW113_003073"
     CDS             1880..2599
                     /gene="atpB"
                     /locus_tag="PW113_003073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2527310.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            2652..2867
                     /gene="atpE"
                     /locus_tag="PW113_003074"
     CDS             2652..2867
                     /gene="atpE"
                     /locus_tag="PW113_003074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2527311.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            2958..3482
                     /gene="atpF"
                     /locus_tag="PW113_003075"
     CDS             2958..3482
                     /gene="atpF"
                     /locus_tag="PW113_003075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2527312.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            3469..4011
                     /locus_tag="PW113_003076"
     CDS             3469..4011
                     /locus_tag="PW113_003076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2527313.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            4034..>5112
                     /locus_tag="PW113_003077"
     CDS             4034..>5112
                     /locus_tag="PW113_003077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2527314.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAG"
BASE COUNT         1702 a          898 c         1099 g         1413 t
ORIGIN      
        1 atcaaacgtg gcgcgatttc ttttgaggat cgcgaagcga aaatccttgt tgatgctgaa
       61 ggacacccaa atgacatcca gctaagaagc agaggaattg gtgaacgctt gatcgaatct
      121 ttcatgcttg cggccaatga aacggtagct gaacattata ataaattaaa tctgccgttt
      181 atttaccgaa tccatgaaca gccaaaagaa gaaaaaatgc agcgcttctt tgattttgct
      241 tcagctttgg gcattctggt ccgaggaacg aaaggaacga tcacaccaaa agatttacag
      301 catgtgatcg aaggagtaga agacaaacca gaagctgcag ttatcaatac tatgttgcta
      361 agaagcatgc aacaagcacg ctattcagaa gacaattttg ggcattatgg cttagcggca
      421 gattactaca cacactttac atctcctatt cgacgctatc ccgatctaat cgtccatcgt
      481 ctgatccgtt cttacagtga agataaaagt gaaagcaatc aaagttcgtg ggctgcggaa
      541 cttcctgaaa ttgcagatca tagttctaag atggaacgtc gtgcagtaga aacagaacgt
      601 gaagtagatt ccatgaagaa agcagaattc atgatggaca aagtgggaga agaattcgat
      661 gggatcatca gctctgttgt gaaattcggc ctgtttatcg aactgccgaa tacagtagaa
      721 ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga aaatcattta
      781 gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacaaaaagt gcgcgtaaaa
      841 gtagtcaaag ctgatccaga tgaacgagca gtggattttg aactcgtttc cgcagaagag
      901 gtcactgcgt tggaacgtcc aaaagcacgc aatggaaaat ctggaaaacg agaaggaaaa
      961 caagcaggca aacgctcaga aaaaaatggc aagaagaaca aacgatatag tgatagtaag
     1021 caaacgttcg gtaagaaaaa agggaaaaag ccattttaca aagatgtcaa aaagaagaaa
     1081 aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg aaaactaatt
     1141 gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga agcagggatg
     1201 gtcttgcaag gaacggagat caagtcgata cgaaacagcc ggatcaatct gaaagatgga
     1261 tttattcgcg tccgcaacgg ggaagctttc ttgcataatg ttcatatcag tccttatgaa
     1321 caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt acacaagaaa
     1381 caaatcgttc gtcttgaaaa cgaattgaaa aacactggaa tcactgttgt tcctttaaaa
     1441 gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcaaaagg gaaaaaatcg
     1501 tatgataaac gggaagattt gaaacgaaaa gatatcgatc gacaaattga tcgaacatta
     1561 aaaaatttct ctagataatt tcattatcct ttttcgcata ttcggtgctt ttgcttttac
     1621 ttaatgataa aatttgttag catttaatct gataaaacat gattatcaca ggttttttct
     1681 tagatcagga gcaccgtgca tggggaaaaa ttcgcttcgt ttttgactga aataaaaaag
     1741 tgtctaattt tcatgaaaat gttttgaatt ttctgtttca tggtggtatg ataccgtgtg
     1801 gttagaaacc gattggaaaa aaccagatac gcacatgaat tgattttatg tgaatttcgg
     1861 ggaaaagagg tgaaatggat tggatgaacg ctcgctaacg tttcacattg gaccaatttg
     1921 gtttgatggt acagtttgta taatggtgtt actgacctgt ttgattgttt tcttcttagc
     1981 ttactttttt acaagaaatc ttaagttgaa accaacaggc aaacaaaatg cgatagaatg
     2041 ggtcattgat tttagtcgag gaatcgtaac agataatctg cctagaaaag aattgagcaa
     2101 tttccatttg ttggcattta cattgttttt atttgttttt gttgcaaaca acataggttt
     2161 agtaacaaaa atcgtcttac ctaacgaaac aacactttgg aaaagcccga cagcagaccc
     2221 gtttgttact ttaactttgg catttataat gatcttactt actcacttat tcggggtgaa
     2281 aaagttaggc ttcaaaggat atttcgtaaa ttcgttcttg aaaccctata gttttatgtt
     2341 tccgatgaaa ctgattgaag aatttacaaa tttattaaca cttgctttac gtctttatgg
     2401 gaatatttat gcgggggaag ttttgctaac cttgattgca aaaatgatga caagcttagg
     2461 ctggttctca ttaccattag caattccatt agagatggtc tggatcgcgt tctccttgtt
     2521 tattgggagc atccaagcat ttgtctttgt cactttgtca atggtttata tgagccataa
     2581 gattgaagta gaagaataat actcggttaa aaaacaaact actatttatc tattttagga
     2641 ggaaaacaat tatgaattat atcgcagcag caatcgcaat catgggagca gcaatcggag
     2701 ccggttatgg taacggtcaa gttatctcta aaacaatcga atcaatggct cgccaacctg
     2761 aaatgtctgg tcaattccgt acaacaatgt ttatcggggt agctctagtt gaagctgttc
     2821 ctatcttagg ggtagttatc gccttgattc tagtttttgc cgtttaattg aatcagtatt
     2881 aaggcagaaa caacatcgtt tgttactgct tttttacatg gaaactagaa atactccaaa
     2941 gaaaggaagg ctcgcctatg ctgaatcaat tggcaattgc agaagttggc aatgcgatgt
     3001 taggtaatat catcgttgtc agcggctcat ttttgatact actggctctc ttgaagcact
     3061 ttgcttgggg accaatcagc gatattttga aaaaacgtga agacaagatc gccaatgatt
     3121 tagattctgc agaacaatct cgcatcaact cagcgaaaat ggaacaagaa cgcgaacaac
     3181 aattgttagc ctctcgttct gatgcagctg atatcatcaa aaatgcgaaa gaaagtggag
     3241 aattaagccg ccaaaatatt ttgaaggatg ctcaagaaga agcagctcgt ctaaaaagca
     3301 aagcccaagc tgatatcact gtagaacgtg attcagcgct gaactctgta aaagacgacg
     3361 ttgcagaact ctctcttcaa atcgcggaaa aaatcttgaa caaagaatta tctccagaaa
     3421 tgcatgaatc attaatcaat caatacattg aaggtctagg ttcttcaaat gaaactagat
     3481 aaatatacag taggaagacg ttacgggaaa gctttatttg aactggcgat cgattcaaat
     3541 caagcagaag aaatctatca agatctcgta agtttacgtc aaatctatga acaagtacct
     3601 ggattaggga atatgttaag tgatgttcgt ctagaacctc atgaaaaacg agaaatcatg
     3661 gacaagcttg tcagcggtta tgatgggatc gttaaaaact ttttagaagt tgtttatgag
     3721 tataaccgaa tggatgacct tttgttcatg atcgaagaat atgaacgtcg atatgatgaa
     3781 catcaagggt tactattggg aagtgtcaca acagcagtcc cactttcaga cgaacaacga
     3841 atacaattag aaaagaatgt tgcaaaaaca atggactatc aatcagttga attaaaacaa
     3901 atcgttgatt cttctattat tggtggcgcg attgttgaag ccaaccatcg agtaatcgac
     3961 ggaagcattc gcacgcaatt agaaaaaatg cgtaatcaat taaacagata gtacaaagag
     4021 gtaggtgaat cgaatggcca tcaaagcaga agaaattagt gccttgatta aagaacaaat
     4081 tgaaaattat caaaacgtgc tttcagtcga agaaatcggt accgtaactt atgtaggtga
     4141 cgggatcgct cgtgcacatg gtttagaaaa cgcgatgagt ggtgagttgc ttgaattttc
     4201 aaacggctca tacggaatgg cgcaaaactt agagtcaaat gatgtaggta tcattatcct
     4261 tggcgatttc gagtccattc gtgaaggaga taaagtaaaa cgaacaggta agatcatgga
     4321 agttccagtt ggagaggcct tgatcggtcg ggtagtcaat ccgctaggtc aaccaatcga
     4381 cggactaggt gaaatcgtta ctgacaaagc tcgtccagta gaagcgatgg caccaggtgt
     4441 tatgcaacgt aaatctgtta acgaaccaat gcaaacaggg ctaaaagcga tcgacgccct
     4501 tgtgccaatc ggacgcggac aacgtgaatt agtcatcggt gaccgtaaaa cagggaaaac
     4561 ttctattgcg atcgatacga tcatcaacca aaaaggtcaa gatatgatct gtatctatgt
     4621 agcaatcgga caaaaagatt ctacagttcg tacacaagtt gaaacattga aaaaatatgg
     4681 cgcaatggat tacacaatcg ttgtgaatgc cggtgcgtct caaccagcac cattgcttta
     4741 tatcgcacca tatgctggta ctgcaatggg tgaagaattc atgtacaacg gtaaacatgt
     4801 attgatcatt tttgatgatc tttcaaaaca agctgttgct tatcgtgaac tttccttgct
     4861 acttcgccgt ccaccaggtc gtgaagctta tcctggggat gttttctact tgcactcacg
     4921 tttattagaa cgtgcagcaa aattaagtga tgatttaggt ggcggttcaa tgactgcctt
     4981 accatttgtt gaaacacaag ctggagatat ctctgcttat atcccaacaa acgttatttc
     5041 gatcactgat ggacaaatct tcttagaaag cgacttgttc tacgcaggta ctcgtccagc
     5101 ggttgatgcc gg
//
LOCUS       DANKHJ010000154         5009 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000154 DANKHJ010000000
VERSION     DANKHJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..909)
                     /locus_tag="PW113_003078"
     CDS             complement(<1..909)
                     /locus_tag="PW113_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL2527315.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFL"
     regulatory      complement(933..977)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1192..2160
                     /locus_tag="PW113_003079"
     CDS             1192..2160
                     /locus_tag="PW113_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2527316.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2273..2551
                     /locus_tag="PW113_003080"
     CDS             2273..2551
                     /locus_tag="PW113_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2527317.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2669..2905
                     /gene="secG"
                     /locus_tag="PW113_003081"
     CDS             2669..2905
                     /gene="secG"
                     /locus_tag="PW113_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2527318.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3008..3763
                     /locus_tag="PW113_003082"
     CDS             3008..3763
                     /locus_tag="PW113_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2527319.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3766..>5009
                     /locus_tag="PW113_003083"
     CDS             3766..>5009
                     /locus_tag="PW113_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HDL2527320.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGEL"
BASE COUNT         1658 a          879 c         1095 g         1377 t
ORIGIN      
        1 taaaaacttt cggaaaaaaa gacgagtatc tacttttgtc ataattttac tgttagcagg
       61 tttctgccaa aattcataac gatcaaccaa tgtttgtcca cgacttattc cttgagtttc
      121 cacagcagtg aagcttttga aaccttcaca gatggcctcc tcgataaaat aagcaacagc
      181 taacggatca ttgatgacac atcctaaaat tctttcttgt ttccagtgga agtcaaaata
      241 aaaacgagtg atcgctttta gatactctcc ctcttcagga ttcatttgac agcagtattc
      301 gaggatagtc ggtgtcaaga cgatttctct tgtcacatct aacccaacca tttctatcgt
      361 ttgtttgaga ttctcgaata cataagaagc tgcttcagga tcacaccagt aattgtattc
      421 agcaactggc gaacagttac catggctttt gtaagttcca cccatcgaga caaaacgttc
      481 catatgtttc ccaatatttg ggttgacttt caaagcagaa gcgatattgg tcaaaggacc
      541 caaagcaatg actgatgtat ttgtcttttc tttgaaataa tcagccaaaa aatcaacggc
      601 atggagaggt tctgcctgtt ttctcaagat catcgggaaa tttgtttccc ctaaaccatc
      661 cataccatga gtgtcttgtg cactgatgaa aggttgcttc aaaggttttt ccattccttg
      721 ataaacagga atgtctaaac gatcacagcg ttccaggcat ttcagcacat tctgcgtacc
      781 tatatctgca gggacattcc cacaaactac ggtaatagca actacttcga tatcagggga
      841 tttgacagct aatgataaag ctaaagtgtc gtcgatccct ggatcgcaat caataattac
      901 ttttcgcatt atttccaatc tcctttcttt agagatactt agttttttat tctgggaagt
      961 ttccgaacca gtccaagacg agcgtcttga ttataatgca cgaaattagt atactggctt
     1021 ttattgcgaa tgtaaatgct ctcttacaag taaatctatt gaggagaact ttaagtaaat
     1081 gatacaatga aaaagaaatg gaatttacgt gtgccgaaaa taaagaatcc tatggatcgg
     1141 tacattggat aatagataga ttatagtttg gcagaaagcg gcgggtgtac aatggttatt
     1201 ttttatttca ttcttattct gataattata gggatcagtt accattttct ttcttatttt
     1261 tatgcaaaga aattagtaag agcgggagta cagagaggat atgactggta tgaagacact
     1321 ggtcatcgtt tgatggaccc tgctgcagtc acgtcagtag aaaaaaaacg acaaaaatta
     1381 gaagaagagt gtgaaagctt ttgggagcag ggaacaccca aaacaatcaa aagttatgac
     1441 gggttgaaac tagctggtca gatgttcatc catccaaata agcagaataa atgggtcatt
     1501 tgcgtacatg attaccgaag cacagggaaa agagacatgt ctcatatagg taaaagatat
     1561 gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga aagtgaagga
     1621 gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat ccaactcatc
     1681 ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg agcttctacg
     1741 atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat tttagacagc
     1801 ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac tgtctttcct
     1861 aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg ttactcctta
     1921 aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt agtcatccat
     1981 ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa tgctacggca
     2041 ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc gatgaaagat
     2101 ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac gatggattaa
     2161 ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga tctggagact
     2221 gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc atatggaaat
     2281 caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga ttggagaaat
     2341 gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg tagatccagc
     2401 atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat tagcaagacg
     2461 agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg aaaagaaacc
     2521 agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt tgaactttca
     2581 gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa gttaaagaga
     2641 cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa tcgtgatcgt
     2701 tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa atagtgcagc
     2761 gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc gcggctttga
     2821 agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct tattatttgt
     2881 cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc gggcggcttt
     2941 ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt aaaatgaggc
     3001 gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac atggaaagcg
     3061 agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa tgctcgcacg
     3121 atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac acggaacaat
     3181 ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca ctaaaaaagc
     3241 catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat tatcaatggg
     3301 cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg gttttttctg
     3361 ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat acgtacgtca
     3421 agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag ttgcttaccg
     3481 ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg tagaaagtag
     3541 gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg aaatgatcga
     3601 ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga cacttcaatg
     3661 gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat tagaaaaaga
     3721 cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac aaaagaaacg
     3781 ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt ttctatggaa
     3841 gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact agtacaaacg
     3901 attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa agtgaaactt
     3961 cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg atttggattt
     4021 gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac tggttttgca
     4081 atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga tatggatcgc
     4141 ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt agcaggggaa
     4201 ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt tgttatcccg
     4261 aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt tcgtccagtt
     4321 gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta tgcgactagt
     4381 ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat ggatatcctc
     4441 tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt gtctgaagca
     4501 gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga tttaagaaat
     4561 cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc agttacggtt
     4621 aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt ttcttactat
     4681 gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag tgtatatttg
     4741 actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg ttcactgaat
     4801 ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa tggacaagta
     4861 gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac ctacacagct
     4921 gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc tcagctcgta
     4981 cctatgttcc aagaaatggg tgaattgca
//
LOCUS       DANKHJ010000155         4972 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000155 DANKHJ010000000
VERSION     DANKHJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            87..572
                     /locus_tag="PW113_003084"
     CDS             87..572
                     /locus_tag="PW113_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2527321.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(676..1269)
                     /gene="clpP"
                     /locus_tag="PW113_003085"
     CDS             complement(676..1269)
                     /gene="clpP"
                     /locus_tag="PW113_003085"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2527322.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1449..1976
                     /locus_tag="PW113_003086"
     CDS             1449..1976
                     /locus_tag="PW113_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527323.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2067..2804
                     /locus_tag="PW113_003087"
     CDS             2067..2804
                     /locus_tag="PW113_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2527324.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2808..3725
                     /gene="miaA"
                     /locus_tag="PW113_003088"
     CDS             2808..3725
                     /gene="miaA"
                     /locus_tag="PW113_003088"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2527325.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3727..4956
                     /gene="hflX"
                     /locus_tag="PW113_003089"
     CDS             3727..4956
                     /gene="hflX"
                     /locus_tag="PW113_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2527326.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1691 a          850 c         1111 g         1320 t
ORIGIN      
        1 tttttttctt atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta
       61 caaaaaatga aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga
      121 ttggtgatgt gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg
      181 acagtaattg gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg
      241 tcgatcatga tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc
      301 tacatggctg tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc
      361 atgcggttat tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag
      421 ttattcctcc taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa
      481 aagaagaaat acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc
      541 atgcgcaagg aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga
      601 tggtcagttt gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc
      661 ttcctttgaa acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc
      721 atatgctttt gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac
      781 ttcaagcggt tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa
      841 aatatggcgt gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg
      901 atggatcatg atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag
      961 caagaagctt cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac
     1021 aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat
     1081 ataaagatag atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat
     1141 cgaattagca agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa
     1201 acgtgagtag atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat
     1261 taaattcata atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt
     1321 tctaatacct aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg
     1381 caaattagtc gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga
     1441 acaacagatt gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg
     1501 tctatatcgg catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg
     1561 aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg
     1621 agatcttgca aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg
     1681 attgttatat cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt
     1741 tttatctgcc aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga
     1801 ttttatctcc agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga
     1861 ttttagagaa ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga
     1921 agactatgac gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt
     1981 ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac
     2041 aatagaactg aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg
     2101 gcacccaccc agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg
     2161 gtatcgaact agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag
     2221 aagtggaccg gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca
     2281 aaaaattgaa tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat
     2341 tgagagaagt gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa
     2401 ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa
     2461 gtagagattg gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa
     2521 tcgcattact ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga
     2581 tccaactagc agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt
     2641 tcgaacatcc agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg
     2701 atgaagaaga aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc
     2761 cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat
     2821 tagtgatcgt cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac
     2881 tatttggagg agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg
     2941 gtacagcaaa aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga
     3001 tcgaacctac tcaacgttac tcagccgctg attttcagca ttcaggtcga caattgatta
     3061 ccgagattgc gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc
     3121 aatcccttct atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa
     3181 aaaagtatga aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag
     3241 aaaaagaccc ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag
     3301 ctctggaagt tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc
     3361 aattgtatga ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa
     3421 tcaatcaaag agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt
     3481 atgagcttgg agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt
     3541 ttgaagggaa agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat
     3601 acgcgaaacg tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta
     3661 taagtgagcc aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg
     3721 aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac
     3781 ttttcagagt caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta
     3841 ttcacaatta cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa
     3901 ttacaagaat tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat
     3961 gagctaactc cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat
     4021 cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg
     4081 caggttgaac ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca
     4141 ttatctcgtc ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca
     4201 gaccgccgac atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa
     4261 gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt
     4321 attggctaca cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact
     4381 tattcgaaag atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct
     4441 gaaggatttg agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa
     4501 ctgatcgatg ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat
     4561 gtagtcgatg ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg
     4621 gatgaactga atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc
     4681 gacccagcga tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa
     4741 gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct
     4801 tatacacttt ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca
     4861 atggttttgg acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca
     4921 aaaaccaatt caaaatggtt aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DANKHJ010000156         4933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000156 DANKHJ010000000
VERSION     DANKHJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            85..972
                     /locus_tag="PW113_003090"
     CDS             85..972
                     /locus_tag="PW113_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2527327.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="PW113_003091"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="PW113_003091"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2527328.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="PW113_003092"
     CDS             2274..2720
                     /locus_tag="PW113_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2527329.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="PW113_003093"
     CDS             2993..4345
                     /locus_tag="PW113_003093"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2527330.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="PW113_003094"
     CDS             4516..4803
                     /locus_tag="PW113_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527331.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DANKHJ010000157         4884 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000157 DANKHJ010000000
VERSION     DANKHJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            509..2353
                     /locus_tag="PW113_003095"
     CDS             509..2353
                     /locus_tag="PW113_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2527332.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="PW113_003096"
     CDS             2409..3875
                     /locus_tag="PW113_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2527333.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="PW113_003097"
     CDS             3912..4754
                     /locus_tag="PW113_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2527334.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          785 c         1028 g         1466 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa atct
//
LOCUS       DANKHJ010000158         4803 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000158 DANKHJ010000000
VERSION     DANKHJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..758)
                     /gene="pfkB"
                     /locus_tag="PW113_003098"
     CDS             complement(<1..758)
                     /gene="pfkB"
                     /locus_tag="PW113_003098"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2527335.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIA"
     gene            complement(755..1507)
                     /locus_tag="PW113_003099"
     CDS             complement(755..1507)
                     /locus_tag="PW113_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2527336.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(1666..1875)
                     /locus_tag="PW113_003100"
     CDS             complement(1666..1875)
                     /locus_tag="PW113_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527337.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(1886..3214)
                     /locus_tag="PW113_003101"
     CDS             complement(1886..3214)
                     /locus_tag="PW113_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527338.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            3350..3505
                     /locus_tag="PW113_003102"
     CDS             3350..3505
                     /locus_tag="PW113_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527339.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(3579..4166)
                     /locus_tag="PW113_003103"
     CDS             complement(3579..4166)
                     /locus_tag="PW113_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2527340.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(4249..>4803)
                     /locus_tag="PW113_003104"
     CDS             complement(4249..>4803)
                     /locus_tag="PW113_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2527341.1"
                     /translation="EFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
BASE COUNT         1404 a          959 c          811 g         1629 t
ORIGIN      
        1 gcaatcattg aatcccctgc acctactgaa tttttcaatg ggcgaactaa tacattcgtt
       61 ttataagttc cttgttccgt gaagagcaaa gcaccgtctc cagccattga gatcagcaca
      121 ttctgtgccc cttcagctag caatttttta ccatacggaa tgatttcttc tacagattgg
      181 aaggttgtat tgtaaagctc agctaattcg tgattgtttg gtttgatcaa caatggtttc
      241 ttaacaagtg cattttttag gtcatcacct gttgtatcaa tcacgaaatc tgcacctttt
      301 tctcgaatga tatcaatcag ttcttgataa aatccttttc gcaggctagc cggcgcgctt
      361 cctgacaata cgacgatatc tccttgtcct actgttccta atgcttgctt caactcatcg
      421 atttcttcat ctgaaataga tgggcccaaa ccgttaattt cagtttcagt atctgatttt
      481 agtttgatat tgattcttgt atcttctttt acggttgtga acaaagactg gatttgttct
      541 ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc tagtgctgta
      601 gattgagaat cgatccgttt caaaatacgg gaaacattga tcccttttcc tccaggtaac
      661 ttgaagtctt ccttcattcg gttcaaatcg cctagtttta acccatctac gcgtacgata
      721 taatcaattg atggattaag tgttactgtg taaatcattg tattgcctcc ttaattgttg
      781 tcttttcacg gaattcatta acgaattcta atggacaacg ctcggttatg atcgtggctg
      841 cttccaaagg tgcaacagag gtgaaggaca actggttgaa tttggtatga tcaatcaata
      901 caaatgtctc ttcggaattt ttcatggcta aacgtttaac agctgcctct tctgggtccg
      961 gagtcgtgaa tccttgttca agatgggcac cgttcattcc cataaatgct ttattaaaat
     1021 gaaaattttt tagttgttct atcgcattag atcctaaaac agcattagtt gccagcttta
     1081 tcgttccacc taaaataatg gtagcaagct ccaaatcaac taaatttgcc gcatgttgaa
     1141 cggaatttgt cactactgtg atctgttttc cttgcagaaa aggaatcatt tctaacgtag
     1201 tagagccagc gtctaaataa atgacatctt cgtcattaat ggtttcagct gccgatttgg
     1261 cgatttgctg tttttcgtga acgtttttga gtgatttttc actcatattc tgctcaaaat
     1321 tcaataattg ctctttctta gctccgccat gaactctttc tagaaaccca ctattctcca
     1381 gctcttgtaa atcacgtctt atcgttgatt cagaagcatt aaaaaggttt acaagatctt
     1441 gtgatttcac gattttctgc tgttcaagca agtctaggat tttttgtcta cgttcctctg
     1501 taagcatttt cacgatcacc tcaatacaaa taatagcata ttttttttct actcgcaata
     1561 taaatcagtc aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaat aaaaaaccag
     1621 tcaaaaagac tggtttttta tttttttgtc tatcatcaag ccgttttatc gttttttatt
     1681 ttcgcttctt gtttttttca tataaacaga agcaggtttt ctttccttat gaagaagata
     1741 ataataaagt cccatcccgc caaacatgac caaaaaaccg atgactgatc gaataggaaa
     1801 attaggaaca aaaaagagac agacggataa taatgatgcc ggaaccatta ttcgataata
     1861 gagttgttta tccattttct tcacctcata ttttttgtaa agtaatcaat tgttctaatt
     1921 ctcggtaatc taaaaattgc acacgcagat gctgctcacc tttcttgaca tcgaattgga
     1981 tacttcgccg ctttttcttt attcgtttga aggtaacatc agagagtcta tgtttgattt
     2041 cctttaacgg taattctgac agggattctg aaggaacata gattcttaag gataaaagat
     2101 actcttgata tttttcatct gttacctcta cttctagata ttccctaata taataacaat
     2161 aagcttcgat tccattatcc aaagcaacag tcggatcgat aactttatag cacatcatgc
     2221 gcgagcgatt ctcgtcaacc caaggaaaat acgcctgagc atttagtgtt ccgcgaacac
     2281 gcctttcttt ataatctcct tgttgaatat cataactttt tgttacaaaa aatgaagcat
     2341 tctttttagg aacaggttgc atttgtgcat tctgatactg gacagattgg atcgtatggt
     2401 cagtgtaaat gtattcaacg aatggcggtt tcaataaacc gacaccattg tcccaagcaa
     2461 gatgatcaaa cccaagctgg tccctaaaat aggattgaaa catttgattg tttggctttt
     2521 gccagttaaa ggggtctcga taaagctgga tacatatgac ttcgttttct aattccttca
     2581 ctttttctac ttgttggatg tcgagaaaaa tcggtttagg gatgatttga tgataatagc
     2641 gactataata catttcccaa cacgaagtga aacataaacc ttggtaaaaa agatcgtatc
     2701 ctggaaattg gttgcaatcg actactactt ccccgttttg ttgtttcatt ttaggtaaat
     2761 gttcgatttc atcctttttc tcaaacaata gccgttgaga gatctgtttt gtattgtgat
     2821 agataaactc gctataagct cgcatatagg cgaacacccc atgagcaatc atttttttct
     2881 gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac agagatagac aatacatttt
     2941 ttatcaataa ttgattatct tccaagcgaa aactacattc gttctcgtca tttcctacaa
     3001 ctaaccaact ttcgtctctt accgatgcta gcattatttt ttcttgaagc tttcgaagat
     3061 catacggatc cgcttgttgt gtgaaagagc ggataaatgt tggctggatc aacagacgtg
     3121 tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc ttcatttaag tctgtgagct
     3181 ggatagtcat caataattta tcttcgccaa tcattttctc acctctttaa aatcttttta
     3241 taatttaatt ataaccaacg ataaagaaaa actaaaactt tatgtcttag aaaccaattt
     3301 ggacgattac atgatagact aagtaaaaag aaacaaagag gtgtttacaa tggggttgaa
     3361 atttgaacgt acagataatt tagataaact tttcgaatca tttgctgtcg atccggaaaa
     3421 aacagttgca ccaaaagaag agatcgatcg atttctaaaa ccacagccac aaaatgaaaa
     3481 gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa agacttacca gccaaacttt
     3541 tatgaaagag tttgactggt aagtcttttt tattcttttt atgattcaat tgcttgttcg
     3601 attgctgccc acgcttcttc ttttccctgt tttgtaacgg aagaaaagag aataaacgtg
     3661 tcattgcgat caaaatccaa cgcttttttg atttcgctct catgcttgtt ccatttacca
     3721 cgagggatct tatctgcctt agtggcaaca acaattacag ggatatcata atacttcaaa
     3781 aattgataca tctggatatc ttcttgagaa ggcttatgac gcaaatctac taaagaaacg
     3841 accgcacgta gctggtctct agtcgttaaa tatgtctcga tcatctttcc ccattttgct
     3901 cgctcagttt tggatacttt tgcataccca tatcccggaa catcgacaaa atgtaatgcg
     3961 ttctcgatca aataaaaatt caatgtctgg gtctttcctg gcttaccgga agtacgggct
     4021 aaattctttc gatcaatcaa tgtgttgata aaagaagatt tccctacatt tgatcgtcct
     4081 gccagagcta tttctggcaa ttgtgtatct ggatattgtt ttggtgccac tgcacttatg
     4141 ataatttcag cttgatggac tttcatggtc tctctcctta acaaggtgat gaataaagca
     4201 agcggctgtc gcaaaagcca aaatgacttt tacggcagcc tcttttcatc aacccgcttt
     4261 tttctctgaa tctttataga ttactgttgg ctcaccagac aattcagctg cttcttttgt
     4321 gataatcact ttttcgatgt tttcatttga agggatatca aacatcacat ccatcatgat
     4381 ttcttcaatg atggaacgaa gtcctcgagc accggtattg cgttcgattg cttttttcgc
     4441 aatggctctc aacgcttcag gttcgaattc aagttcggta ttatctagag aaagaagttt
     4501 ttgatattgt ttgactagcg catttttcgg ctcagtcaag atccgtacca gatcatctgt
     4561 tgtcaatttc tctaaagctg ccataacagg taatcgtccg ataaactcag ggatcaatcc
     4621 gaattttagt aaatcttcag gaatgatatg ctgcatgaca ctttcgtctt cagaaagttt
     4681 ttggttttgt gtaccaaatc caatcgtctt ttcgcccata cgattcttca caatcgtttc
     4741 gatgccatca aatgcaccac ctacgatgaa caatatgttc gtcgtatcaa tctgaataaa
     4801 ttc
//
LOCUS       DANKHJ010000159         4760 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000159 DANKHJ010000000
VERSION     DANKHJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(513..998)
                     /locus_tag="PW113_003105"
     CDS             complement(513..998)
                     /locus_tag="PW113_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL2527342.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(1000..1764)
                     /locus_tag="PW113_003106"
                     /pseudo
     CDS             complement(1000..1764)
                     /locus_tag="PW113_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1934..3127)
                     /locus_tag="PW113_003107"
     CDS             complement(1934..3127)
                     /locus_tag="PW113_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527343.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3211..3411)
                     /locus_tag="PW113_003108"
     CDS             complement(3211..3411)
                     /locus_tag="PW113_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL2527344.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3835..4068)
                     /locus_tag="PW113_003109"
     CDS             complement(3835..4068)
                     /locus_tag="PW113_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527345.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4075..4506)
                     /locus_tag="PW113_003110"
     CDS             complement(4075..4506)
                     /locus_tag="PW113_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2527346.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1229 a         1125 c          911 g         1495 t
ORIGIN      
        1 tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc atctaacaaa
       61 ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta gccaatatcg
      121 tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat ttcaaccatg
      181 aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt atagagttaa
      241 tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa gttgcgtggc
      301 gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga ttaaacattt
      361 tttatcatag agttttactt atctatagaa ttatgtctgt tcctaattca gtttgatgaa
      421 ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt aaaaaaataa
      481 catgtcatta gatagtagac agttccaact ctttatttac cttttttgta tgctgcactg
      541 tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata ttggccatca
      601 ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat tttaattact
      661 gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt aacggccaaa
      721 atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc tcgatcaaga
      781 gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt atctatccag
      841 ttttttatag caatatctcc attacgttta actttctcaa attcagcctt atctacaaaa
      901 ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc tcttgttaca
      961 tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct gaatactatt
     1021 tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat ctaatttttc
     1081 tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat tctcttgaat
     1141 cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta ctatattttc
     1201 attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta tttcacctga
     1261 ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa tgttttctcg
     1321 atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa gtacgtcttg
     1381 tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa tactattacc
     1441 aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc tatatgctaa
     1501 tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat caacagcact
     1561 tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca aaatatcagt
     1621 aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca aatcattgat
     1681 ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa aatcctcaga
     1741 atcgacaaaa ttttccttta aaattatatg ggattttaga ttatcttttt agaagttctt
     1801 gaatgtcggt atagcccgtg tttccgggct tttccggcat ttttgatatt gttagatact
     1861 tacaaatgtt cttattacct ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa
     1921 aaccggcctt gtgtcaggct gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg
     1981 cgtgtgcata atagttcagc gtcatggtga tattggaatg tcccataatg tactgcaagg
     2041 ctttggggtt cattcccgca ttggctaacc gggtgcagaa tgtatgacgc atagcatggg
     2101 gcgtcgtcgt tttcggcaac gcttcttcat ggctcttgtt gtacttcttc acaagcctgc
     2161 caaacatccc gccataatct accgccgtca tgggatagcc gttctgtttg agaaacagga
     2221 aattggcata cccgtcaata atgaacggct ttgcaccttt gcggtttttt atcactcgct
     2281 ggaacgcctg atacacttcc tcattcatgg ggatttttct gacgccgctt tgggtcttgg
     2341 gttcgtcaat atagtaaccg acgccggtat ttctcaaaag ctggtggctc acgatgatta
     2401 cccggttttc aaaatccaaa tctttgtccg tcagtccgca cagttccgaa atgcgaagcc
     2461 ctgtccccag cagaataacg attgcgtcat aatgcttttg gtaaacctta tcaccttgaa
     2521 taaaggacag aaaactttct tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt
     2581 cgtccagaac gtcactaagc tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta
     2641 tggcggtgta aaaagccgct ttcagggaac gcttgtcgtt attgatggtc ttataagaaa
     2701 ggcccttttc tttcatccga agcgcccatt cctttgcgtc ggacagcttc acactgtcaa
     2761 tcgggcagct ccccagccga tcttgttcca gaatacgcat cagccgttcc cggcccagtt
     2821 tggtgttgtg cttcacattg ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg
     2881 tcattttctt gccgatggtg tctatcccgt cgtcaaggtc tttctggatt tctttctcct
     2941 tttcccggag tgaaatatcc tcccgtttac ctgccggggt cttatccgta ggcactaact
     3001 tccatgagta aacaaactgc ggttttccaa aggtatcaac atatttgtaa gcatatcgtc
     3061 cgtcttttct ctggctctct ccagaccgca aaacgcgatt tttgctgtct cgtctttttt
     3121 ctgacatctc gtcatgctcc tttccatgac ggaaagagcc gcatatatgc ttataaatta
     3181 ttataacata tctgcggccc tttttcacta ttagattgtg tccaaagtgt caattatttt
     3241 ctcaaactgc ttgcgtttga tctggatgcg gttgccattc ataatgaccc agccagaggc
     3301 cggattttcc tcggccagct tacgcaattt gttttctccg atacggaaat atttagacgc
     3361 ttcctcaatc gtcagcgtgt atttttccca aataggcaca tcatggttca taatgcagcc
     3421 ccctttcaca aattcaaagg tttgtgtgat ttaggggcat agacggacgg ctgctggcac
     3481 agctccacgg gactttcacc cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac
     3541 gcttttaacg ctcggactgt ggctataagg gagtatcata gtcctgcctg tacgtcgtcg
     3601 cccgcaagcc gccacgctca cattgcggcg tggtgttcct cgctccgctt ttcccgtagg
     3661 gaaaagtatc atggcgcacc agccgtccgg ctcggcacag acagaatgtg tccgtatgcc
     3721 ctatgatgcg ccgccgttat cttgcgggca tatttatcaa ggtgcgatca ggccaccggg
     3781 aaaacaccga aacggggaaa gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt
     3841 cgtgacgata gcgtggatga gcttgatttc cagacggcgc ttcatggcct cgtccagaca
     3901 cacatgcgga tagccgtttt cgtcatagag cgtccgggta cacagcttgt tcatgtaacc
     3961 gctgtaatac tgcaaaacgc gctctacggc tccggcatcc ccagcgcggg cggcctcgat
     4021 caccgaaagg ggcaaaagct caatgccctt gtagttcggg ttcttcatcc gcgtctaacc
     4081 tcccttcggc atcaacgcct ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg
     4141 ccgctgaacg gcactccggg acattcccac aaggccgccg atctcaccat ctgccatatc
     4201 cagaacacag tgcaggatta aaatactctg ctccatgctg ggcagcgcgg caaaggcttc
     4261 ggcaacaagc tcattgttga tatgtaggtc atagccatgc gacgaaaacg taaagctgtc
     4321 gctggggtag tgatctactg tacagagctt gtccaaatct gcctgtgaca aagagcttag
     4381 tgacacttca cggtcacggc ggcgcttcat ttcagccaag tagcttttgg cctcgttacg
     4441 cagtaccgtc ttgcaaaaag catcgaacat gcaccgcgct tcatagtcgc gggggatagc
     4501 atccatcttc tcaccccctt tctgtggggg acttaggtgg ttctctccct ttccgctaac
     4561 aagactttga caaggccgcc agctacccgc taaatacctc ctttttgaaa atttctcaaa
     4621 aaattttttt ggggacagcc gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa
     4681 acctaccggg cgagaaaatg ctttgctatg ctgctgattt acatggtata gtacatactc
     4741 ataaccggaa tagccctgtg
//
LOCUS       DANKHJ010000160         4720 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000160 DANKHJ010000000
VERSION     DANKHJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1097)
                     /locus_tag="PW113_003111"
     CDS             complement(<1..1097)
                     /locus_tag="PW113_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2527347.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="PW113_003112"
     CDS             complement(1227..2603)
                     /locus_tag="PW113_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527348.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="PW113_003113"
     CDS             complement(2603..3259)
                     /locus_tag="PW113_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527349.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="PW113_003114"
     CDS             complement(3269..4546)
                     /locus_tag="PW113_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527350.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="PW113_003115"
     CDS             complement(4565..>4720)
                     /locus_tag="PW113_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2527351.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          857 c          567 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DANKHJ010000161         4696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000161 DANKHJ010000000
VERSION     DANKHJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            500..1201
                     /locus_tag="PW113_003116"
     CDS             500..1201
                     /locus_tag="PW113_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2527352.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1773..2789)
                     /gene="tsaD"
                     /locus_tag="PW113_003117"
     CDS             complement(1773..2789)
                     /gene="tsaD"
                     /locus_tag="PW113_003117"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2527353.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2786..3250)
                     /gene="rimI"
                     /locus_tag="PW113_003118"
     CDS             complement(2786..3250)
                     /gene="rimI"
                     /locus_tag="PW113_003118"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2527354.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3257..3799)
                     /gene="rimI"
                     /locus_tag="PW113_003119"
     CDS             complement(3257..3799)
                     /gene="rimI"
                     /locus_tag="PW113_003119"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2527355.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3783..4505)
                     /gene="tsaB"
                     /locus_tag="PW113_003120"
     CDS             complement(3783..4505)
                     /gene="tsaB"
                     /locus_tag="PW113_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2527356.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1405 a          969 c          777 g         1545 t
ORIGIN      
        1 tttccctatt tcagagaaaa cgtgaatagg tttatccatg ttgtgttgcc tccttctttt
       61 tgattacatg tataggatat cctccttttg taatcacttt cataattttg tctcattcaa
      121 aatgagacat tcctcacatt aatattcgat aattaaataa ttaaacgttt ttctattttt
      181 aagcgtaatt tattcatttt ttattctgta tacactttca aaaaaatatt tattcattga
      241 aaaaaatttt ttgaataaat taaaaaaata aattatatta gcgttgttag cgtttactat
      301 atgtattatt gttgtttttt atacacaaat cattcgcttc aaaacaaatt attcaatacc
      361 attgtatatt ttttacataa taacaaatta tatttgtaaa gtattaaaac ttgtttctaa
      421 tgttggtatg ttaaaacttt acaaattctt taaaaaaagt gacaataaat atagtgaaca
      481 ataaggagag gactttttaa tgcgtgttaa taaaatcgat tttcactttt caaaactcag
      541 atatcaacct gattttgaac atttctcgga tgaagagttc caacagatca aggaacatac
      601 tgttcttcgt tcttataaaa aaggacagat ccttttcgac gatggtgatc ccagacaacg
      661 gttgtatatc ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa gtgcaactta
      721 ttactactat gactatgtga atacaaataa cctttttcct atgtcaggaa tgtttggaga
      781 agagacctac acttatacag ctcaagcgat gactgatatt gaaacattct accttcctgt
      841 ttcctactat gaaaaattag cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa
      901 aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa atcggattga cctctagcgc
      961 ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa gaattgggcg taccaacaaa
     1021 agatggtcat atcaccattc cttatccgat caccttaaaa gaattagcag tgaatagcgg
     1081 cacgacacgt gaaactgctg gacaagtaat caaacaacta cgtgattccg gactgttaga
     1141 ttatgacaaa aagcgtttta ctttctgtaa gacagctact actccagcta tgagagaata
     1201 acgcgaacaa aaataagcag tgaccctgat atttatcagg gtcactgctt attttcctcc
     1261 ctaaaacttc tgaaagatcc ggcagtttta gatcactggg caagactcaa agattttcta
     1321 tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa tactgataga ttaactagag
     1381 gaagcgtctt gtcctcatcc gacctagatt attcgatatt cctcgacaat aaacgattaa
     1441 tcagtgaggg caatcagagg aacggataat gatgaataag ctgtaaaaaa tagggctttg
     1501 acaaaagcgt gtcacagccc ttatttcgat taaacggtgt gtacctgtca actcttcggt
     1561 cacaagtcat aatgttcata aacatgagga gctgtttttt gaaattgttc cttgtgaatc
     1621 agagttaaac gacaggctta cgaactcttg attaaacggc gttcaaaagc ttgcatacgc
     1681 ggtaataaga acagccaggt caaaaacatg agaagccgtt tttcctgggc tgttcttatt
     1741 acttgatgca gggcagattt ttcacaacct ctttattcag ataaaaccaa gcttggatca
     1801 gcattcaact gatagctagc aaaatttctt ttttgcattt ccacatgtgc tgctgctcca
     1861 atcattgcag cattatctcc acaaagtcga agagggggaa tcactagttc tacctcaggg
     1921 agtttagttg ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc cgctacacct
     1981 ccagctacaa tcagttgctt gactggataa ttttgacaag ctcgaagtgt tttagtaatc
     2041 aatacatcga tcacacttgc ttgaaagcta gctgctaaat catttttgtc tagatcttct
     2101 ccacgctgtt gtgcattatg caccaaattg ataaatgcac tttttaatcc gctaaaactg
     2161 aaatcataat tatcttcatg gatcatcgct cgtggaaaat ggtaattatc ttttccctga
     2221 tgagctagct gatcgatttc ttttccgctt gggtaagaaa gacctaatac tcttccaact
     2281 ttgtcataag cttctcctgc tgcgtcatct cttgtctccc caatgatttc atatgatcca
     2341 tcttcttgca tatagactaa ttctgtatgt cctccgctaa caagaagtgc catcaatgga
     2401 aactggaatg gtttaaccag acgggctgca tagatatgcc ctgccatatg gtttacagga
     2461 ataagcggca attggtgtgc ccaagcaaaa gccttagctg cagaaatacc aatcagcaac
     2521 gagccgacaa gtcctggtcc ataagtgaca gcgactgccg ataaatcttc tgccgacaca
     2581 ccagcttcta ccagtgcatc ttccaagcat aaggtgattt gttctacgtg atggcggcta
     2641 gctacttcag gaactacgcc accaaaacgt ttatgactgt ttatttgaga agcaacgatg
     2701 ttagataaga tctctgttcc atttctaacg accgctacac tcgtttcatc acaacttgat
     2761 tcaatcgcca gaatcaattc ttcattcatt tcatcgtctc cgtttttaac tttctgctca
     2821 taatgatcgc atcttccgtc ggattgcgat aatatgattt ccgtttacct aatatacgaa
     2881 acttttctga ttcataaagt tttcttgctg cttcattaga tagacgtact tctaaaaaaa
     2941 catgcagcga atcttttatt tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc
     3001 cttttccttg ttctgaaact gcaacagcaa tatttgtgat ctcgacttca tctattactt
     3061 tcgagtatcc aataaagcca cagatctggt tatcttcaac taaaagcaga taatcagagt
     3121 gaggctgttg gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca tgtggataag
     3181 cattttcgct aatcatccat acgcattctg ccaagtacgc ttcatcaaaa tctttttttc
     3241 tgcaaatcaa ctaatctcac tcattttcta aaagttttat catatccaac gcatcttctt
     3301 ttgtttcagt gtaatatctt tttttgatcg ttgttgcttg aaaacctaac ttccgataaa
     3361 gtctttgtgc atcttgatta ctcatacgca cttctaatga catcgtctca cagcgattca
     3421 ttatagcaaa gttctcgatt tcgtcaataa gcagcgaacc gattcgttta cgctgatatg
     3481 ccggtcgaac ggcaatattc gtgatatggc aatctaaacc gaacgctctt gaaccgataa
     3541 aaccaatcat tcttccatta tcaaagatac ctagatatag atgaggaagc ggtgacttca
     3601 attctgatat aaaggcactt tttgtccatg gcaattcgcc attatagact tctcgttcga
     3661 catccagcag ctctttgata tcttctgtag tcaattctct tagtacgtaa cgacgatcga
     3721 tttgttcgat tgttttttct gtataaatca ttttatcttg aaaaaagctt ctggcatacg
     3781 ctctaaattt tttcaacata actttcgtct ccaggtgtgt gcgtctccaa ccatttttct
     3841 tctgcttcta cccgttttaa atagcgagga agaaaagctt gtttctctgc aactggttca
     3901 gctaaagcac ctaattgagc cacagttaca ccacttggaa tatcccatgc tggaactgta
     3961 ttaatcatcg cactaggcaa tatttccttt atttcataga agaatttgtg acaatcactc
     4021 ccgacgaaat aaatagcttc attcagcttt tgtagttgtg tgaacaaatc agaagcagaa
     4081 atatgtacat cttcaagtac aatttctaat ctattttctt tccagcggta tgccccagcg
     4141 tatacatttt ttcttcgagc atcaaacatg ggcacaataa ttttatccac accgatgcaa
     4201 ttagccgcga tggttttcag actagatact cctactagct ctttatctaa tgtataagca
     4261 atcgtttttg ctgtcgttac acctatacga agccctgtat aagaaccggg accatcagaa
     4321 accgcgattc ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa ttggtcgatt
     4381 gcaggcatca gtgttacact gtggttgcgt ttgatatttg tttgtatttg tccaagaatt
     4441 tgttcgtctt ctaccacacc gatcgccaaa gtttggtttg cagtatctat tcctaatgta
     4501 atcatatatt tgatcctttc tgctatgaca ttctcgcctt ccattgtagc atactttttt
     4561 attctgagga tgaaaatacc aaaggcaaaa ctagtacaat gatacatgat tatctgctgg
     4621 cgggcaaatt ttgccccttc ctgaaacaca aaaaagctgc ggtatcactt catttcccca
     4681 cagcttttcc atcttt
//
LOCUS       DANKHJ010000162         4678 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000162 DANKHJ010000000
VERSION     DANKHJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            1040..1366
                     /locus_tag="PW113_003121"
     CDS             1040..1366
                     /locus_tag="PW113_003121"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527357.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1347..2282
                     /locus_tag="PW113_003122"
     CDS             1347..2282
                     /locus_tag="PW113_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2527358.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2911..3162
                     /locus_tag="PW113_003123"
     CDS             2911..3162
                     /locus_tag="PW113_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL2527359.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3704..3862
                     /locus_tag="PW113_003124"
     CDS             3704..3862
                     /locus_tag="PW113_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527360.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            3934..>4678
                     /locus_tag="PW113_003125"
     CDS             3934..>4678
                     /locus_tag="PW113_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527361.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYID"
BASE COUNT         1560 a          857 c          816 g         1445 t
ORIGIN      
        1 aaaatattta aataaacccc tttcaaaagc atttctaggt tttggacaat aaggaatgac
       61 ttttttcttc atataaatac ctcatttaac tatttgatat catcacatta tcaaaaaaaa
      121 taggtttttt attaaaacta tcttgacctt tcgggagatt tcactgtgat ttatttttat
      181 tcccatactt ttttcgttgg tcactttcgt atacctaatc taagcgatca tcggcgacgg
      241 tgcttttaca acgaatggca ggacaaaaca aggggcggaa aaactttttt gcctatttca
      301 taaaaaactt tttcctacag caatcccttg ttttgtccta ccacacgttg tgaacccaaa
      361 gcacgccgac gaacgcttag ctatacgaaa atgacctcca aaaacaaaaa agtgaggtga
      421 atagaaacaa aaagaaattg aaatctccca aaatgtcgtc cgcctcggag acaacttgct
      481 gctgaaaatg gttggttaat tcatttaagt attaaataag ataaatcatc aaattaatcg
      541 gaaaaatagc atgattggcc ttatcaaaaa taaactgtaa aaacttatat ttctatgagt
      601 ggtggaacaa tgctaatacc aaattaattt atcgattacc cacctgcaca aaccaaagat
      661 agcattatta ggaaacgaat caatcagcga atccagcatt ataatgagga tactaaaaat
      721 aaagttttca taattataaa tccgccttcc tacgaaaggc accaatgagg tgatgaaaac
      781 cttagagcgc tttctttctt tttttattcc gtgttaccct ttccttgcag gtccctggta
      841 cactacagaa cccgaggagg tgagtggcgg ggctgtatag cggcaacccc gcataattat
      901 atggtgtcgg tcatccgtta aggcatcaat gaatggcgca aacccgtagc ccgtaaactt
      961 atctcttccg aagttgactc gatttcgccg gatgaccagt ccctaccaaa cctgtactat
     1021 ttttttacag gaggttgcta tgttcaaaat ctttcgtaaa aattgttgtg gcttggatgt
     1081 ccacaaaacg tggatctatg cgtgtatcgg aatcacagat gcgaatggtc gtacagtgta
     1141 taagcaagct cgcttctctt cctttacaaa aggtctcaat gaattagctg aatggcttgc
     1201 taaatacaac tgtaaggatg tgtgtttgga atcttccggt aaattttgga ttcctgtttt
     1261 taatattctt gaaaaaactt gttttgtcac actggctcat ccgaagtaca ccaaacccca
     1321 gaaaggaaat aaaacagatc gcaaagatgc aaaaatggat ttgtgatctc tttatgtgcg
     1381 acatgatcaa acccagtttt attcctggtc ttgaaatccg ccagcttcgt gatctaatgc
     1441 gttatcggac gaaactgacc aacatgaaaa caagtgaaaa gaatcgtgct ttaaactgct
     1501 taaccgtttc taatctgaaa ctagatgatg tttttagtga tgtgttcgga aaatcatcca
     1561 cttctatcat ccaatacttg ttagaacatc ccggtgaatc atttgatgtt cgaccattta
     1621 tccatgcccg ttgtaaaact cccgttgaag aaatccaagc ggcagttgag ggggcgattt
     1681 ctgtacctca agcgattaag cttcgccaat gcctcaacca tattaacgag attgagaagc
     1741 atcgagaaga gatcaatcta gagattcaag ctcttgcaga acccttctcc cctgttttgg
     1801 aactacttcg taccataccg ggcttagaca ctcagccaat aacagcgatt gcgattcttt
     1861 cagaaatagg accggatatg tccgtgtttc caacttcgaa acactttatt tcttgggctg
     1921 gatgctgtcc aagaaacgat cgcagtgccc gacgaacaaa atcaacacgt atctctcgtg
     1981 cagggcagtc gttgaaaccg ctacttgtac agattgccaa tgctcttctc agatccaaag
     2041 aacatccaga atttaaaaat cgctatcgta gaatcaaagc gaatcgtgga cacaagaaag
     2101 ctatcattgc tatttgtaag atgctcctga ccgctatctg gaatgtctta tcaaaggtgg
     2161 aaccctattc tgctaaagga tatctggagc caaaacctgc gaaagaagag aaagttctga
     2221 cgcaatccca agctctggac cttttaagaa aacgcggata taccattata gatagtgctt
     2281 aaaccaaagt tacgttgatt cttcctaaat agactcgttg tcttccattt caaaaatttt
     2341 gaaaacggta ggcttattta gtgtaccgtt cttcaggtat cttcaaatag atttattttc
     2401 aaacttattt cctttcttct tttactctcc tatagcttag catttaagtg ggcactcgtg
     2461 tcaccttgct agatagctat gaatgccgta ataagcctcc tagtatatag gcagactgcc
     2521 aattataact gattcaatcc acattatgat aaaatccacc ttttcgtttc atatgtgttg
     2581 aaatatcaca tatgtcaaaa gtgcacgagt cttttcattc agtgctttgt aaatactcaa
     2641 aattattcct tttcacttta aaaaaaagat aattagtaca aaatcagaaa tgaggaaagt
     2701 attaggatgg actagaaagg agttgttgaa tttgttgcaa cagattggtc atttatcaat
     2761 taaaaattag gtgaagtttt attcatcact gcagctgctg tttcccagag aataaaacgt
     2821 ctagaagaaa ctggtcagtt actgtattat caagcagcaa ttgactacga acaagcttga
     2881 ttactaacca aagcgtttat ccacctgttg attaaatcaa atcaaaaatc aggtttttat
     2941 acgttcataa aagcaatctc cagtgtactc gaatgtgact gtatcatagg tccttattcg
     3001 atgttaatca aaatggggtt tccttctacc gaatcattgg acagttttat caatgattta
     3061 tcaaagtttg gacgaaccaa cacccaaatc gttttctcga ctcttgtttc cttaatggtt
     3121 tatcagttct ggaatgttta tcatgcgctt ttattaaaat agaaaagagc ttttttcatg
     3181 cgacaaagca gtgatcattt attggcacat gcaatctttg tggatccatt ttttgaaaat
     3241 acgatattta gttttgaccc aaaaatcttt aaatacgtat ttgataattt atcttaacaa
     3301 ccagataatt ttattcagca ccgtattatc tatattgaat tctcggaatc aagataaatt
     3361 tttttccgtt tttgtttctt ttttaatctt cttcttgtta ttaatagtga ttaggatgat
     3421 attcctatat tgatctttga taattagata taatttttga ataacgttcc ctgctattcg
     3481 agctagaaat acaagtacgc catgacctag ttagcgacaa ctacttagag atttcttttg
     3541 ttctgggaca acaaaatgcc atgacaatag cagtaaataa tgatacttcc gtaaggctcg
     3601 ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcggttc aaaaatttta
     3661 aactattatt ttaggagatg attttataaa atcgaaagcc tacttgaata aaaaacaagc
     3721 ctgtgaattt ctaggaattg ataataatac tttagataag tggattcgtg tatataattt
     3781 tccagtaata aaaattgatg gcgtagttcg ctttgacgta gaggatatca aagatttcat
     3841 gaatacattc aaaaaaaatt gaaattttct tctcgtcaca tatactataa atagcgaaat
     3901 gtactgcctt actttttaaa ggagtgagga agtttgccta caaagctttc aaatgggaaa
     3961 tataaaacta atctgaggta tccaaaaaaa ttcagagaaa tcactggaat tgcatcagag
     4021 aagtatcaaa aagtatttcc aactaggcaa ttagctatta aggccgaaag ggaaatgaaa
     4081 aagaagattg aaacagtttt aagagaggag aatgcaaact cactagaatt gaaaggaaaa
     4141 ataaaattta aagattttta tgaatcaaaa tggcttccta gatatgaact aggtcaaact
     4201 actaggtcaa acagggctcc aagtaatgta acaattagta atactaagga tatattccga
     4261 ttgcacatcc ttccaatgtt tggtgaatat gcaatgaatt atctgaattc aaatactgag
     4321 ataatatcag atgaattaac aaagaaatcg aaagaatacg caaatataaa aattatcaaa
     4381 ggctatgttc gctctatgtt tgatattgcc gaaatactat gttatatcga attcaaccga
     4441 actaccaaaa ttattcaatg tattaccgcg cctaagaaaa tggctttaga agaaaaaagg
     4501 ataagggaag ggaatcaagc attatcatca gaagagctga ctgattggat agaagcagta
     4561 aacaatgact taaataatca cttgcttaca cttcacgatt acacactttt catgcttacc
     4621 ttatatctag gtgatagaaa aagtgagact tatgctctgc aatggaaata tattgatt
//
LOCUS       DANKHJ010000163         4663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000163 DANKHJ010000000
VERSION     DANKHJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..588
                     /locus_tag="PW113_003126"
     CDS             <1..588
                     /locus_tag="PW113_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2527362.1"
                     /translation="KHDITTITAASTDAKAAKYVQELFMNNYFRIYTNPDVIGVEMGA
                     ALKNIIAIGAGAIHGLGFGDNAKAAIMTRGLAEISRLGVAMGANPLTFIGLSGIGDLV
                     VTCTSVHSRNWRAGNLLGQGQPLEEVLANMGMVVEGVNTTQAAMELAQMMEIEMPITQ
                     TIYEVLYEQKEIKTAAKEIMLRDGKKENEFSLTIF"
     gene            603..1541
                     /gene="galU"
                     /locus_tag="PW113_003127"
     CDS             603..1541
                     /gene="galU"
                     /locus_tag="PW113_003127"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2527363.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            1666..2106
                     /locus_tag="PW113_003128"
     CDS             1666..2106
                     /locus_tag="PW113_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2527364.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            2109..2711
                     /locus_tag="PW113_003129"
     CDS             2109..2711
                     /locus_tag="PW113_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2527365.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(2770..3876)
                     /locus_tag="PW113_003130"
     CDS             complement(2770..3876)
                     /locus_tag="PW113_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2527366.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            4086..>4663
                     /locus_tag="PW113_003131"
     CDS             4086..>4663
                     /locus_tag="PW113_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2527367.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDP"
BASE COUNT         1594 a          839 c          994 g         1236 t
ORIGIN      
        1 aaacatgaca tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta
       61 caagagctgt tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta
      121 gagatggggg cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta
      181 gggtttggcg ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt
      241 ttaggtgtag caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat
      301 ctagtcgtta cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga
      361 caagggcagc ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat
      421 actacccaag cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa
      481 acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg
      541 ttacgtgatg gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt
      601 atatgaaagt taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag
      661 caacaaaagc tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca
      721 tcgttgaaga agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta
      781 aacgaccaat cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa
      841 aaggaaagac cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca
      901 tccgtcaatc tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg
      961 gaaatgaacc atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga
     1021 cacagcaatt aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag
     1081 ttcctcatga tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg
     1141 ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt
     1201 tagctattat tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac
     1261 aaccaggagc cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac
     1321 aacgcgtatt tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt
     1381 ttatgaaaac aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg
     1441 agtacatcct gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc
     1501 caaaaggaat aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct
     1561 tcagctgcta attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga
     1621 aaatgctatc cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa
     1681 attgcaggat tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg
     1741 ttgttgcaag tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg
     1801 acgatcgaaa tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg
     1861 gtcaaaagta atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg
     1921 ttcacagcag ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac
     1981 atcgcgacga aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa
     2041 gtcggtggga cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga
     2101 aaataaaaat ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg
     2161 cagcaatcgc cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag
     2221 ctgcccaagc ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa
     2281 aatcgactga gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag
     2341 gtgatctagc aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg
     2401 aatctgacga tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca
     2461 aaaaaacggc agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata
     2521 cttcagaaga tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca
     2581 atgaaggaat gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg
     2641 caaaagaaga tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta
     2701 caaatcacta aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct
     2761 tttttactct tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca
     2821 gccatcgctt gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata
     2881 gattcctggt tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac
     2941 aagggaaggg aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata
     3001 ttcgccgtag ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt
     3061 cacacctgga cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat
     3121 tttttcttgg aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata
     3181 gccattccaa acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt
     3241 tcctggtgta ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga
     3301 catttgtgaa acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc
     3361 agtcacgcct tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc
     3421 agcttcgatc agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat
     3481 ggaagttaga tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg
     3541 ctcgacagtc aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg
     3601 aagttctttt ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata
     3661 cgcccaacta ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc
     3721 ttgttcaaga gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga
     3781 aaagtaagcg atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat
     3841 ccattgacgt agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa
     3901 ttttgtattc aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa
     3961 aaagtaaaaa ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat
     4021 attctatttt agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa
     4081 cacaaatgga aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga
     4141 tggctactgt ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa
     4201 aagtcttgga agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg
     4261 caagtaagaa aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg
     4321 cttctttagc acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag
     4381 caaattcaga tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc
     4441 aagtggacgg cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt
     4501 cacgttcaaa aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta
     4561 gcgtaaacat tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg
     4621 gcaataaaaa aatcgctttt gtcagtggtg cgttgatcga tcc
//
LOCUS       DANKHJ010000164         4587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000164 DANKHJ010000000
VERSION     DANKHJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            186..2855
                     /locus_tag="PW113_003132"
     CDS             186..2855
                     /locus_tag="PW113_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2527368.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="PW113_003133"
     CDS             complement(2946..3566)
                     /locus_tag="PW113_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2527369.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="PW113_003134"
     CDS             3680..4219
                     /locus_tag="PW113_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527370.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="PW113_003135"
     CDS             4216..4554
                     /locus_tag="PW113_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL2527371.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          859 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DANKHJ010000165         4521 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000165 DANKHJ010000000
VERSION     DANKHJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..974)
                     /gene="lacD"
                     /locus_tag="PW113_003136"
     CDS             complement(<1..974)
                     /gene="lacD"
                     /locus_tag="PW113_003136"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2527372.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(971..1429)
                     /locus_tag="PW113_003137"
     CDS             complement(971..1429)
                     /locus_tag="PW113_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2527373.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1426..2889)
                     /locus_tag="PW113_003138"
     CDS             complement(1426..2889)
                     /locus_tag="PW113_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2527374.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2871..3803)
                     /gene="pfkB"
                     /locus_tag="PW113_003139"
     CDS             complement(2871..3803)
                     /gene="pfkB"
                     /locus_tag="PW113_003139"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2527375.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3945..>4521
                     /locus_tag="PW113_003140"
     CDS             3945..>4521
                     /locus_tag="PW113_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2527376.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1354 a         1016 c          724 g         1427 t
ORIGIN      
        1 tacttgttaa cataaataga agttgctgtt tcttctaata gtgtacttaa ttctttaata
       61 ttttgttttc cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa
      121 ggtgctactc ccttcgccca tgttgctcgc ccacataaga caccattgaa tgtcgaacct
      181 gattgttttg caaaacgcaa tgtatcttga aatagtgcag cgctgactcc cgcacttaaa
      241 aaaataaacg gttgttcagt cactttcgat tgttcataga aatacgcgga tgcctctgct
      301 tgactataac aagtatcttg cccatatcct tccacaaatc tcatattcac tggtacttct
      361 actttcaata catccacttg ataacgttct tttgaaaatt ctcgcatggc ttcaatcact
      421 ttatgcggtt ttacttttgc atagtcaaga gaagacggat cgccaatagt tgcatcgtaa
      481 gaaaggagct ctaaaaagaa aggtatgcct tctgcttcgc attctgaacc aatccgttct
      541 ataactattt gtttttgcac attaatctct actggttcat caacgtcata gtacaataaa
      601 aatttacaag catccgcacc cacttctttt aaccttttca ctgaccaact attcagtaaa
      661 tctggtagtc ggcctggcat tttcgcatca tagccggttt tttcataagc tagcaacaaa
      721 ccacattctt tctctcgaag cgctgctgca ggcagaccat attctggatc gagtaaaata
      781 gcggaagcat acggagtcag gtactgagaa accatctgtt taaattggat gatatcttct
      841 gatgtagctt ctttatattg gttgatcatt ttcttcaaag caccacgttg gtcgatagct
      901 agcgcgctaa taattccagc ttcatccgtc aatcgcttca aacgttcctg tttttctata
      961 ctcattttct tcatatatct tcacctattt ctttagttaa tattgtagct atttctattt
     1021 ttgattttgc agctctcaac tgttctgtaa cctcttttcg catcaatgat cgtgcaacga
     1081 gagacaataa tttcaaatga gtcgttcctg cttctgaatc tgggataaat aacgcaaaaa
     1141 taaaatcaac tgactgtcta tctaaactgt tccattcaat tccttttttc agtgtgacga
     1201 taatcaaact aggttcattg attgtttgag acttcgcatg agggatagca aaacctgaca
     1261 ttattcctgt agtaccttct ttttctcttt ccagaagttt ttgatatact tctttttcgt
     1321 catgagcgat tcctaaacag aatgtttgct ttgctaaaaa cttaaatgct tcttcttgat
     1381 tatctaatgg ctgttgtaaa aaaatgttct cttcatgaat gattgtcatg ataaacctcc
     1441 tgctcttcct tcttcgataa ctgtacgatt tgttgcttta ttatccctct tcttgtcttt
     1501 atcagttact cttttctttt ggatcaaacc tagtatcact ccaccaacga tcgaaccaat
     1561 aagaatcgct gtaacccatt ggatttttcc cgtcactaca ggtaacacta aaaaaccgcc
     1621 atgtggggct ggtacttgga caccaaacag ataggtcaag acagcagcaa ttgatgagcc
     1681 taacatcatc gttggtaata ctcggatcgg gtctttcgca gcaaacggaa tagctccttc
     1741 cgtaatatga gttgatccta aaataaaatt aaccaaacct gcattacgat cattagtatc
     1801 aaaatattta ccaaacaata aagtagcaaa accagtaatc aacggtggtg caatgcaagc
     1861 tgctgacgca ccagccataa aagtcgtgtt tccttgtgtc aaaagagctg ttcctgttac
     1921 atatgccgcc ttattaaccg gtcctcccat atcaaacgca cacatacagc caacaatcac
     1981 gccaagaaca agtggattcg aatattcaaa tccagctaaa aagctcatca ttccttcatt
     2041 tatagccttc attggacctg ccagcaaaga catcacgatg ccaataaatg ctacaccaat
     2101 aactggatat aagacgattg ccttcaagcc atctagttgt ttaggtaaaa ttatcagtag
     2161 cttttgcaaa aataagataa aataacctgc taagaaccca gctaaaatac ctcctaaaaa
     2221 tccagtaccg ccattcatgg ctacgattcc accaacaaat ccaacgacta accctggacg
     2281 tttcgcgatc ccttctgcaa tataagctgc taaaactgga accatcattc ccattgctgc
     2341 atcgtcaact gcttttacca ttgccgctgt agcattgtat tgtgcatgat ctggatcaga
     2401 agaatagatt ccccaaacgg caaatgaaat agcaatcaat actccgccag ctaccacgaa
     2461 aggcaacata tgagagacac cgttcatcaa atttttatag atgctacgac caaaattagc
     2521 accacttatc gcttctggtt ctaaagtatc agcagcttca ttatctgcta agacagtacc
     2581 ttttcctgcc aatgcttcgt caatcagccg ttgtgcatcc ttgattcctg tactgacaga
     2641 aacatcaatg actcgttttc ccacaaatct ttctgaatgg acatctttgt ctgcggcaat
     2701 aatgacagca tctgcctgtt tgatttcttc agccgttaat tcattctcga tcccaatttg
     2761 tccatgggtc tccactttga tcgtgatgcc ttttttccta gctgcttgct ctaatgcctc
     2821 ttgcgccatg taggtgtgcg caattcctgt cggacaaccg gttgctgcaa ttagttgata
     2881 ttttcccatc tttcgaacct ctcttccttg attttgattt gtttacttaa ttcctccaca
     2941 tccaaaaaat cagtcagtcc tttacgaaaa gcagtagagc tgcctgctgc tatgctttta
     3001 cgtaacgttt catcaagcga tcgtcgattc atgtatcctg ctaaaaaagt gcctaacatg
     3061 gcatcacctg cacatgctgt attgacaact tttccttttg gcgagtttcc tcgaatcacc
     3121 ctgtctttcg taaaaagaat cgctcctttc tcaccgaggg ataacaaaac attttctgcg
     3181 cctgactcta ctagtcgttg tccataaaca aagtaatctt catctgtaac catttttcgt
     3241 ccgaaccaag aagaaagttc ttcctcattt ggtttcagca aaaacggtcg atagggtaaa
     3301 caatccatga cttcttgatc actactatca ataatcaaac aaatccgact gatttctatt
     3361 aaaacctttg gagacagccc tcttggtaaa ctcccagaaa cacaaagata atcccctgct
     3421 tgtaatgctc ttatttgatt taaaaaatta tgaacagctg tctgcggtat ttcaggtcct
     3481 tgattcacta atttgtattc ttctcccgtt tgattgactt gtgtaaatac attgatacgc
     3541 gtcataccag gcacttcaat aaactctgtt tggatttgtt tctgctgtaa atagtcttct
     3601 atatactttc ctgtgaaccc agcagaaaaa cctaatgccg tacttgggaa atctagcatc
     3661 tttaatacta gtgacacatt cacccctttt ccgtttgcct gaatgtcatc ctcttttgtg
     3721 cggtttacca taaaaggatg catctcatct gtttcaataa atagatcaat tgccaaattc
     3781 atcgtgcatg tgtagataga cataaccaac gacctccttg attctattat gaaatacgcg
     3841 aaatagaaaa tgttttctta atcgtcccaa cacaaagaaa acctttacat ttataaactc
     3901 gtcgtgcttt ttttaactaa cttttggtga aagaaggaaa gaatatgaat cacaaattaa
     3961 gtgaaacaga gcagtacctc tggaacttta tcgaacacct catcctagag atccctaact
     4021 attccatcgt gaaactaagt gaacaagcaa atgtctcaac agccaccatt gtgcggacaa
     4081 tgaaaaagaa aggctatgaa gggtttactt cgtttaagca tcatctaaaa gaaggagaaa
     4141 acaagaacat caatttttca tttttagata aagtagacaa aggaatccgc cgagccattc
     4201 taaaaaatga acaagaagtg gtacgtacaa tcaatatgtt ggaaatcggg aacattgaag
     4261 atgcaataca aagaatcaaa tttgctgacc gggtttttgt ctttgcccga ggattttcag
     4321 aaatgattgg ccaagaaatg ctggtaaaac ttcaattaac gggaaaaaat tgtcagatgc
     4381 atactgatcc agaaattatc aaaagcatca gtcggaaact aactaagaaa gacagcgtta
     4441 tttttgtttc tttgaacggt gagaaaacaa ataaaaacat caaaaataag atttatgtct
     4501 ttatttttca attttttaaa t
//
LOCUS       DANKHJ010000166         4462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000166 DANKHJ010000000
VERSION     DANKHJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..996)
                     /locus_tag="PW113_003141"
     CDS             complement(<1..996)
                     /locus_tag="PW113_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2527377.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKE"
     gene            complement(1027..2241)
                     /locus_tag="PW113_003142"
     CDS             complement(1027..2241)
                     /locus_tag="PW113_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2527378.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(2582..2824)
                     /locus_tag="PW113_003143"
     CDS             complement(2582..2824)
                     /locus_tag="PW113_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2527379.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(2856..3413)
                     /locus_tag="PW113_003144"
     CDS             complement(2856..3413)
                     /locus_tag="PW113_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2527380.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(3426..3614)
                     /locus_tag="PW113_003145"
     CDS             complement(3426..3614)
                     /locus_tag="PW113_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2527381.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(3627..4193)
                     /gene="amaP"
                     /locus_tag="PW113_003146"
     CDS             complement(3627..4193)
                     /gene="amaP"
                     /locus_tag="PW113_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2527382.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1358 a          936 c          747 g         1421 t
ORIGIN      
        1 ttcttttgca aaatattcag gaagtaatcc ttcttctgct gctacgtgaa gtgcttttga
       61 aggagaggtt acccaagaag caatttctcc gatgacacct aaacaaacaa gaatcgcaag
      121 aattttcgca atccatgcaa gatttggatc aatgtgtctt aataggaatt ctaacgattg
      181 aatcacccct gtattaagag acaaaccatg ttgaggtact gtagtagcga cggtcaatcc
      241 gccaaaagta tctaaaatga ttgccagaat aaccaataca aacattgcta atggataatc
      301 ctttttaggg ttttccattt catttgcatg actggcagat gtttctacgc ccatatatgc
      361 taaaataaag gaaacaaaaa caaccagcgt aggcaatttc gaaaaatcag gaatcaatgt
      421 tttcatagac agtatcgttt gtagtggagc gcctgaagcg acataatgga tccctaagcc
      481 aaacaagact aatgctggca acaagatacc agctacaaaa ccgatccgag caatcaatga
      541 agtgtatttg gttcctttta actgtgagag taaaatagcc cagaaaataa tcaataggat
      601 tgccaatttc agatatttat tgttttgtat agcaccaata cccgttgcat aggataatgc
      661 accagttaag aaatacagca tggtcaagta gccaactgtt atctcaaacc attggaaaaa
      721 gattgcggca aaaccgaatc gttttcccaa tgtgcgactg acccaagtat agacaccgcc
      781 tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag ggataaacca
      841 tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg cgaatgtggg
      901 atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa agaacgttaa
      961 tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt tctatttgaa
     1021 tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc
     1081 ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg ttgtcagcac
     1141 ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga agtaccagct
     1201 gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt
     1261 gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt gtttcttttt
     1321 acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca gcatggcact
     1381 ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa cggatcctgt
     1441 tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca gccaagtaat
     1501 catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt gttcatctgc
     1561 tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat
     1621 agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct ttctttgacc
     1681 aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga ctgctccacc
     1741 agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc agtggcaaac
     1801 agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt ttaaattcaa
     1861 tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca tcagttggta
     1921 aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga agcttgtaac
     1981 atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat cccgtccaaa
     2041 ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg agagaaaaga
     2101 ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa aaaaagctag
     2161 accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg tatctcctgt
     2221 attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag tataaaagaa
     2281 ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat aaaagacaga
     2341 caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa taaagacaaa
     2401 aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt atttttaaag
     2461 atattttttc ataaggaata aagaatactt catataaaat atataaaaaa gaaccgcagc
     2521 gagtgtcgct acggttctca aatttgttct tcatttcaac ataagttttt cttcaatttg
     2581 cttataattt acgtgcaaag aatgaaacga ctaatactaa aatgattgca ccgatcaatg
     2641 atggaatgat agccattcct gcaattgttg gtccccaagt tcctagtaaa gcttgtccaa
     2701 ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat ccccattgat gatcctttat
     2761 tcgtgattgc tcctgcaatt gctccaatga taccgccgac gattaatgcc caaataaatc
     2821 ccattttaaa ttcctcctaa ctgacttctt acttcttatt gtacacgtgg ttcgttattt
     2881 tctttgactt tttgtgcacc tttgcctaca gcagattttg ctttatctgt ttggcgtgaa
     2941 gcaaattgtc ctgtggattt tgctgcttct gttactttgt cttgtactgt ttcttgatct
     3001 ttttggtatt cttcttgtgt tttgatgtct gcaacattca cattcacttc gataacatct
     3061 aaatgtgtca tattgtttac ttcagtactg atcaattctt tgatttggtt gtaaatatct
     3121 tcgatgtctt taccgtattc tacgatcaca tctagatcca cagctacttg tttcttacct
     3181 acttctgtat tgatacctgc tgttacgtta tctgtgttga ctaatttttc agccatattt
     3241 gagaaaaatc cgccatctaa tgtcaataga ccatctacat ctttcaatgc ataaccgatg
     3301 attttttgga tgactttgtc ttcataagtc aattcaccat tcatcatgcg gttttctcct
     3361 ttttcatttg ccaaaactga tccatttgtt gtattcatgt ttttgttttc catctttgtt
     3421 tcctcctatg atttaaaaaa ttgatcaact attcctgact gtttcaaata aaaaccgatt
     3481 cctgcgccaa ttgctgtcat gattatgatc aacaatgtct tgaaaaatcc aatcgttaac
     3541 cataaaacag ctagcagtag tccaagaccc ccaaagataa tagggagctt gtatgctgaa
     3601 aatagttctt tcattatttg gccctcctat tccactcgag agtgcttgta gtctcttgta
     3661 tcttcttgtt cttcataacc gctgtatgtg actgccactt tgaccgtttc ttttgttccg
     3721 agtactttag ttacttcttc ttcgatatct tgcatcaata cacctgtttt ccctactaat
     3781 gaagaagttc gtttcaagtc tccgttgaca tggatatgga ttcgattttt cgttgaacgt
     3841 atccgtactt taggtgaatg aatgaactct tcttcgtgta aatgggaacg taccaaccct
     3901 tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc gtttcataac gaaagttcct
     3961 cttgactttg gatagaaaag aacgactagc aagatgataa tagcaagaac ggcaaatact
     4021 acagctgccc agaaaatata ttgttgcatg tagtaattcg ttaaagtaaa gaagcgaaat
     4081 cgttccaatt gttcaggcat aatatacggc gcattgatca acgcaatcgc tagaaatact
     4141 gaaagtagca gtaaactcat gataacaccg attattttta accccttatt cattttccac
     4201 ctcctataaa cttattttta ccctgtaaat aactctaccg acaatcacag tatagatagc
     4261 gaacagttag catagcatca gtaaataaag cgctaccaaa cattgctgga tcataacggc
     4321 gttttattgc cagtctgata ctgaaataac aattgcttac ttattctcta tttacttttg
     4381 attgctggct atacttaaag gatattattc aagattaaaa attgcaaaga ttatgctcag
     4441 ttattattac aagattctat tt
//
LOCUS       DANKHJ010000167         4454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000167 DANKHJ010000000
VERSION     DANKHJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..115)
                     /locus_tag="PW113_003147"
     CDS             complement(<1..115)
                     /locus_tag="PW113_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2527383.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="PW113_003148"
     CDS             246..698
                     /locus_tag="PW113_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527384.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="PW113_003149"
     CDS             712..1401
                     /locus_tag="PW113_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527385.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="PW113_003150"
     CDS             1526..3877
                     /locus_tag="PW113_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2527386.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="PW113_003151"
     CDS             3957..4352
                     /locus_tag="PW113_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527387.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1565 a          573 c          885 g         1431 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatt
//
LOCUS       DANKHJ010000168         4434 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000168 DANKHJ010000000
VERSION     DANKHJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(543..2048)
                     /locus_tag="PW113_003152"
     CDS             complement(543..2048)
                     /locus_tag="PW113_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2527388.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PW113_003153"
     CDS             complement(2045..2731)
                     /locus_tag="PW113_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2527389.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PW113_003154"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PW113_003154"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2527390.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DANKHJ010000169         4377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000169 DANKHJ010000000
VERSION     DANKHJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..564
                     /locus_tag="PW113_003155"
     CDS             <1..564
                     /locus_tag="PW113_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2527391.1"
                     /translation="NYHVVDQETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHL
                     SMATAWEILERSNYDKKILFISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVV
                     FDDIGAELGSGVSNSRQFTNNTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRI
                     FKNSEGYALKFQQTADKRIKPVKGSIA"
     gene            561..920
                     /locus_tag="PW113_003156"
     CDS             561..920
                     /locus_tag="PW113_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2527392.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            935..1096
                     /locus_tag="PW113_003157"
     CDS             935..1096
                     /locus_tag="PW113_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2527393.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            1093..1398
                     /locus_tag="PW113_003158"
     CDS             1093..1398
                     /locus_tag="PW113_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527394.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            1398..1715
                     /locus_tag="PW113_003159"
     CDS             1398..1715
                     /locus_tag="PW113_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2527395.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            1712..2098
                     /locus_tag="PW113_003160"
     CDS             1712..2098
                     /locus_tag="PW113_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527396.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            2095..2430
                     /locus_tag="PW113_003161"
     CDS             2095..2430
                     /locus_tag="PW113_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527397.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            2576..3145
                     /locus_tag="PW113_003162"
     CDS             2576..3145
                     /locus_tag="PW113_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2527398.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            3142..3315
                     /locus_tag="PW113_003163"
     CDS             3142..3315
                     /locus_tag="PW113_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527399.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            3312..3617
                     /locus_tag="PW113_003164"
     CDS             3312..3617
                     /locus_tag="PW113_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527400.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            3641..3838
                     /locus_tag="PW113_003165"
     CDS             3641..3838
                     /locus_tag="PW113_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2527401.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            3835..4041
                     /locus_tag="PW113_003166"
     CDS             3835..4041
                     /locus_tag="PW113_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527402.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            4041..4337
                     /locus_tag="PW113_003167"
     CDS             4041..4337
                     /locus_tag="PW113_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527403.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
BASE COUNT         1623 a          659 c         1015 g         1080 t
ORIGIN      
        1 aattatcacg tagtggatca agaaacaaaa atcgctttag aaaaagcaaa aagctatacg
       61 aatgaggttc tgctgaacca tcctgcacat ttcattctat cagggaaatc aggaagcggg
      121 aaaagccact tatcaatggc tacagcttgg gaaatacttg agcgctcaaa ttatgacaag
      181 aaaatacttt ttataagcta tcaagaatta ttagagcaaa taaagttttc ttataacaat
      241 gctgaactga gaaaagaaat tgaaggatcg cttatagccg atattaaaac aactgatttg
      301 gtggtttttg acgatattgg agctgaatta ggtagtgggg tatcaaatag taggcagttt
      361 acaaacaaca cgttaaacac gctcttagaa gcaagacaga acaaggcaac gatcatcaca
      421 acaaacttat ctggtcctga actaagagaa gcctacggtg aaagaattgt ttctaggata
      481 tttaagaatt cagaaggtta tgcgctgaaa ttccagcaaa cagcagacaa gcgcataaaa
      541 ccagtgaaag gtagtatcgc atgaataaat accgtaataa aaaaactgtt catcgaggta
      601 tcaagtttga ttctatcgca gaagcagagt attacgatct agccttgtgg caagctgaag
      661 cgaatggctg gaaagtaaaa cttcaggaaa gatttgagct gatgccgaaa tttgaactag
      721 acggaaagaa gtatcgcaag atcgagtata ttcccgactt cacattttat aaaaacggca
      781 aacttgtcaa agtcgtagat gttaaaggaa tgcagacaaa agactttaag atcaaggcaa
      841 agctgttttg tcatcaatat caagtgccgt tgatattagc taaaaaatat cggaatacgt
      901 tcaaggaaga gcgtttttaa agaggtggtc tatcatgaca acagaagaag tgattcaaat
      961 gcgaattcga agcattcaac gtgagattga cgatctggaa cgaacaaagg cagtgatggt
     1021 caatgaaacg gcgagaaagg caatcgattt gcacatagag aacttaagaa gggaaattcg
     1081 tagattggag gaatgagcgt ggataagaaa gcagcaatga aacgaatcat cgaactgaca
     1141 cattctgaga attggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa
     1201 tcaatgtgga ctgaaaaaac cagacggaga atgccgagaa aaattgcaat ctggcacggt
     1261 gaccgaattc tagtaacagg taccgctgaa cagttatctg aaattactgg tctgagcaaa
     1321 aacatcatct gggatagagc gaagaatatg gatattgatt ctaaaggaag acagtttagg
     1381 tatgtggagg agaaataatg aatctcatta cacaatacag tgatatcatc ctcaagaaaa
     1441 tcatgatgaa gattcagaaa gacaaaaaat caaaagaacg agcggaatta gttaagttgg
     1501 aaatggctga aacaggggca ggagtgcgaa gtagcaggca ttggaaggca gcagcaaaca
     1561 ttgaatttta ttacaacgaa attcaaaaag ggttcgatca aatgcgtgag ctggatcggc
     1621 aaacaaattg gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aaatataaag
     1681 agatattaga cgaatacatg gagggcagca aatgataccg agatttcgat cgtggtacac
     1741 accatttaaa ggtaaaacaa ttggacaaga aatgaaatat gggcaagcag gaaggttgat
     1801 cactcatgct gaaatggctc cagataaata tgtgcttatg caatccacag gactgaaaga
     1861 taagaatggc gtagaaatat ttgaagggga tgtagttctg gttaatgtaa gtaatggttt
     1921 tgatcattta gttaatgaaa aaacggttgt acaagaatcc gagtttcatt ccggattagt
     1981 ttgtaaatca ttaattagtg gaatggaata tagaatattc agccaccaaa agatgggata
     2041 cgaatatgaa gtcaccggaa atatacacga gaatagcgag ttattggagg tagagtgatt
     2101 atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt cccagcattg
     2161 ctaaattgtg gaattaaaag taagaataga atcattttgt tacggatatc ggctgattta
     2221 gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac acgttatcag
     2281 tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc tgacatacat
     2341 ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc cgatgatttt
     2401 ccaaatttat ggttgaagga ggaacagtaa tgaaagactg gttagaagaa gcaagcgcct
     2461 tagttggctg tttcataaac tgtgcgttta tatctgtaat tattgcactg gtgggatttg
     2521 tatcattgaa agtgattata ttgctttggc agttaatttt ttaggaggaa cagcgatgaa
     2581 taaacaggaa gcaatcaaaa agttagaaag tattaaagtg ataggaaatg atgcactagc
     2641 tgcttgctat aacgagagta taaatgcagg tattacgtta atgaaaaaaa tagacgaacc
     2701 gaagaagcta gttattccac aactcgtggc cggttggctt gagaaatcta cggacccttt
     2761 tacgaaagct gaaaaaatag cgtatttaat caaatctaaa gatggtgatt catattattt
     2821 ctgtgattgg tttgtacgag atggcatagt gacgcaagaa caaggagaag aattactcgc
     2881 ttgggcaacg agacaatcat atgaaacact attgagcttg tacaacggct acgaggtcga
     2941 gaaagagccg ttgtacagtg taataattgc tggcgagtac cttgtcaagg agatcagttg
     3001 cagtaatgaa gttaaactag tatcaacagc ttacctgcca caataccttg cacaccacta
     3061 ccaactgacc gaaaaacaga tcaaagcaat tgatgaaaga tactggccat ttgctgtgcc
     3121 agtggaagag gtggcggaag gatgagtaga cagataatgc tccatatacc agacggaaca
     3181 aaggcaatga cgattaccat tgttgctgca caaaaagacg gtgggttggc gttgaaaaca
     3241 aaaagcattg ataccaaaca gattttagaa ggcagagatg tcgagataga gatcgatgag
     3301 gaggaagcgg aatgaatgtt caagtttatt taaaaagaga tttggccagg tttcctcgaa
     3361 gctgggatac aaattcccac ccatccgagg cagaagaaga gggctatcta atgtctgcta
     3421 acatgataga aattacaaga gatgatgcgg atgagtttga taaaaaaaga tcatggtact
     3481 cggttccgaa tccaatgtat acggctgtaa tagaagatta tcatatatcc gacagattta
     3541 ttctgattga tctagaaagg cctaagaaac gaattaaaca gtatagacgt tgtggatttt
     3601 caacaaagaa atggtgataa caaaacagag gtgaagaaaa atgaacaaca gacaccgcag
     3661 agtagcaaaa ctaagaaatc aagaactgaa tgtagcgaaa gtaaagttcg aacgggagta
     3721 tggtgtttct gcagaagaaa catataaagt ggcaagtcag tgtgttgctg atgcaagtga
     3781 tgctattcgt aagtttggaa tttcgatact agatattaaa tgggaggata cagaatgaag
     3841 atttatgtag taaagtttgg aaatcaattt tacagaagcg atgaacgttc tataggggct
     3901 aacacattat ccattgtaga catactccaa agcgcaagat ggtttgataa tctcgaagaa
     3961 gctaaccaag tttcacgacg acttggtgga ttaacgcaag tatatgaact ggtcactgtc
     4021 gatcatgagg gggtagagta atgaaactaa aagacggatt ttacgctagt agtcatggta
     4081 tcggcggttt gatgctagat atgccgacaa agaaccctaa aacacgtgag aaaccaaaat
     4141 tcaaagtcgg tgacatggtt cgctgtgaag cagaaggatt tatctatcca tttcgtggat
     4201 atgtagaaaa ggtattgtcg aactcagcaa tcattcgtat tgaaaacacg atggaatgtg
     4261 acaagtggtt agcgaaaagc aaagagaatt tagctgtagc gagattagtt gacatggaag
     4321 taatcgagag caaataaaaa agccggatcg ctccgactaa cataataaaa cagacaa
//
LOCUS       DANKHJ010000170         4349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000170 DANKHJ010000000
VERSION     DANKHJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            109..564
                     /locus_tag="PW113_003168"
                     /pseudo
     CDS             109..564
                     /locus_tag="PW113_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            661..2910
                     /locus_tag="PW113_003169"
     CDS             661..2910
                     /locus_tag="PW113_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2527404.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            2913..4088
                     /locus_tag="PW113_003170"
     CDS             2913..4088
                     /locus_tag="PW113_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2527405.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4091..>4349)
                     /locus_tag="PW113_003171"
     CDS             complement(4091..>4349)
                     /locus_tag="PW113_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2527406.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1515 a          633 c          791 g         1410 t
ORIGIN      
        1 atgtaataat ataaacataa taaaacaaaa attatccaaa tctaacatag gagggtggat
       61 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga tcaggacaat
      121 ctagtcagtg aattgtccag acaaaaacaa gtttataaat ttttagaact cctcttaaat
      181 tcagctagtt atctgaacga agggataatt actaaaaaga tgaatccttt tgttcgtcaa
      241 acaattgaaa taatacgtga gaatgcgtat caaaaattat ctgttaaaga tattgctgtc
      301 agtctatcga tacacgaaaa ttatttatca cgattgttta agaatgatat gggaatatca
      361 ataaaagagt atattagtga aatcaaaatt tctattgcaa acgatctgtt atcatcaact
      421 gaatattcaa tccagcaaat cagtgacaag ataggattct caacagtaca atctttttct
      481 aaagccttta agaaagcaaa tggtgtttca ccgggagtat atcgaaaacg tagattaggt
      541 ctaggaatca ttaatggtga ataaactaaa catttgattt tgataaatat tcgttacttt
      601 aaacgcttac tatttatgtg agcgtttttt ataatgcaat aaagaaacgg aggagagctt
      661 atgtataagg ataatgaaat gaatattatt tatcgatcag aaaaccaaca atttcatttg
      721 tttaatcagg agataagtta catttttaag atatcggaag atggtaaatt attgcattta
      781 tattatggtg agaacttacc agataatgac tatagtcatc taattgagat gcatcatcgg
      841 cctatgacaa cctatagaaa tgaaaacgat ttgctatatt cgttagagca cttaaaacaa
      901 gaatttcctg aatatggtac aacagatttt agacatcctg ctatttcatt actacagaaa
      961 aatggctctc gcttatcaga ttttgtctat caagggtacg aagtggaaga aggaaaacct
     1021 aaactagaag gtttacctgc tacctatgtt gataataatg atgaaagcaa aacattgaaa
     1081 gtttatattt tagattcagt tacaaatgtt caagtagaat tattatatac aatttataga
     1141 gattatcctg ttattactcg tagcgcgcga gttatgaata aaggaaaaga tattcaaata
     1201 atcgagaata ttagcagttt atcattggat ttacccgatg caaattatga atggcaacaa
     1261 ttatctggtg cttggggtag agaaagaact attaagtcgc gtactttaca acaaggaatt
     1321 caatcaatcg aatctactcg aggtatttca agccatcagc ataacccatt tgttacttta
     1381 aaaagaaaaa atactgatga aaatcaagga gaagctattg gtgcagcact agtttactca
     1441 ggaaacttct taattcaagc agaagtagat acatgggatg ttactagact acaagttggc
     1501 atcaatccat ttggatttaa ttggaaactc aatccaggag aaatatttac agcaccagaa
     1561 gcgattctgg tttattcaag taagggttta aatgccatga gtcaaacttt tcatcaacta
     1621 tttagaaaac gcttagctcg tggtaagtgg cgagaaaaag accgtcctgt cttaattaat
     1681 aattgggaag caacatattt tgattttgac gaagaaaagt tggtaaatat tgctaaaaaa
     1741 gcatttgatg ttggtattga attatttgta ttagatgatg gctggtttgg agaaagagaa
     1801 aacgattgtg caggattagg agattggcat gtaaatccta aaagactacc tagaggaatc
     1861 ggcagccttt cagaaaaaat tcgtagtttt ggtatgaaat ttggtttatg gtttgaacct
     1921 gaaatggtga ataaggatag tgagttatat cgcacacatc cgaattggat tatttctaca
     1981 cctaatcgaa aacctaatca tggtaggaac caatatgtat taaattttgg gttagatgag
     2041 gttgttgaaa atattttcga tcaaatgtgt aaaatcattg atgaatcaaa cttagattac
     2101 attaagtggg atatgaatcg tccattgact gatgtatttg acagtcactt gtcagcagat
     2161 caacaaggag aagtattcca cagatatgta ctaggcgtat atcgcttata tgaaaaactt
     2221 ataacaaaat atcctaacat actatttgag tcttgttcat caggtggtgg gcgctttgat
     2281 cctggaatgc ttcactatgc acctcaagct tgggctagtg atgattcaga tgcaattgaa
     2341 aggttaaaaa ttcaatatgg aactagtatg ctgtatcctc tttcgtctat tggtgctcat
     2401 gtatcaattg ttccaaatca tcaaacaaac cgtataacac ctataaaaac tagaggaaat
     2461 gtagcttttt ttggtgcatt tggatatgag cttgacttaa atgaattaag tgaaggcgat
     2521 ttgaggattg taaaagaaca aattgccttt tataaaaaat atagaaccat aattcataat
     2581 ggtacttttt atagactatt atcaccattc gatgatgata atcaaaccgc gtggatggtt
     2641 gttagtcatg ataagaaaac ggctattgtt gctcattata aaacattaaa tgaggtaaat
     2701 gctccttacc gccgactgaa attaaaaggg ttagataatg acggacttta taatgtagga
     2761 caaaatagtt tgcgatctgg aatggaattg atgagagcag gactagtctg ttcagatgca
     2821 tctagtggac aaataaatga tcagaatcga cgcccagaaa catttgattt tgattccaag
     2881 atttgggtta taacacagga ggaaaagtag agatgaataa ccaaaaaagc ttacttcaaa
     2941 aaggagcaat cttaagtatt tcactaatgt taacaagctc tcaagcaatt aatggtgtta
     3001 ttccacaaat gaaacagtct ttggggatat cacaaagtca aagtgaactt ttaggaactg
     3061 caccaagtat aactgttatt ttgtttatct tattatctag ttactttgcc aaaagaatag
     3121 gaatgaaaaa gacaataatt ttaggtttat tattagctgg tataggagga attattccag
     3181 tatttacagc taccttccaa accgttctta ttagtcgagt aatattaggt gccggtttag
     3241 gagtatataa ttcattggct gtgacatata tcagctcttt atactctggc aatactagag
     3301 caacgctatt aggtattaga aattcaatgg aagctttagg tcagacaatc cttatttttc
     3361 ttgctggagt tttagtgaac attaattgga cggcttcgtt tttagtctat gcaattgcgt
     3421 ttcctgtagc tctcttattc gcgctaaaag ttcctgaaat tagagatgaa aatagtgaca
     3481 tacctgaaaa tcatgtaaaa gaaaagatga atccaatggt ttttgcccta gttctatttg
     3541 caatcttact cgtaatgaat agtattgcta tttctgtacg atttgcatca attgcaactg
     3601 aaattaaagg ggtaaacttt aatgctagta actatttagc tctaatgcct atcttaggaa
     3661 tactagcagg atttatgttt ggaccaataa ataagtggat aggaaaagga accctatact
     3721 tgggaattat attctttata atttcaaatt tgttaattgc tgtgtcaaac ggtaatatga
     3781 cgttcctgct aacgggttta tttttatcaa gtataacagg atcatggtgc ttcccattta
     3841 tctttagtaa cttagataaa gtcactacaa agaatacaat taattttgct acttcattga
     3901 tttttattgg ctgtaatatt ggtaatttta tagctcctat tgctatgcaa ttagcacaat
     3961 ttctaacaag aagtacaaat ttgactgctc cattttttgt attcgcagga atatttattg
     4021 tgatcctatt tgtaacattt tttgcgacaa ataaaaaaaa tactagagta acccgtggtg
     4081 ctagttaatt ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc
     4141 gacttaatag aacactgtaa gccagtaata tgctttcaaa tctactctct agccctttaa
     4201 cagaacgtga gttgaaattt tgacaaccta atttttctaa ggaagaaaaa acagtctcaa
     4261 ctgtttttct ttgttttcca agtaagctgt ggtctagttt ttctgattta tccatgtttt
     4321 tacgtggcgg aacggatata gtaatgccc
//
LOCUS       DANKHJ010000171         4259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000171 DANKHJ010000000
VERSION     DANKHJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..2016
                     /locus_tag="PW113_003172"
     CDS             <1..2016
                     /locus_tag="PW113_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2527407.1"
                     /translation="DEEQEITREIKNQNITDSDLIVELSKRIFDGVLDRNSFAYEKLG
                     MSGGNKFKNRYTFTYAQVLDQRPYKQAGNAEMTVNFISPYSDLAGQEAELALKSSRNL
                     LFVELPSDKEFKDDIAMYARMNLYLTTTYSSVVKNFDAIRSEKSGELQELSASINDQL
                     NELLKSAKFFYNGAEIQLTGSDFKAKFQSALQKMAEEVYHKLNQIDAPKEKEDILYLV
                     KESKNEIIEDPSNEQAVQEIFNFINDQKRNHLTITMKTIRDRFETKQPYGFTELDVEW
                     CIAKLFMSGKIDLLYNNVQISRMDDPNSIVMIIQKRRESEKIKIEPSAVISPKAKKLV
                     DEIGRELFQSRNIVDENNNEHTMENFKKYLKGQLDNLRSYFDLQNTYTYPSAYEYEQI
                     KKYLEVIVQTSDVEDFFYTIKKYRDELFDWAETYDKIKNFHKQNSTQKKIWENAQDKI
                     RLIEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQEFIEQYNQLLELESQ
                     EILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQANTIDDLVFLSLNVA
                     EQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDSAAKAEEIAIVKPTIQ
                     QKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEITIQF"
     gene            2080..>4259
                     /gene="pglX"
                     /locus_tag="PW113_003173"
     CDS             2080..>4259
                     /gene="pglX"
                     /locus_tag="PW113_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2527408.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRK"
BASE COUNT         1681 a          570 c          782 g         1226 t
ORIGIN      
        1 gatgaagaac aggaaattac tcgtgagatt aaaaatcaaa atattacaga tagtgatttg
       61 atcgtagaac tatctaaacg aatttttgac ggcgttctag accgaaatag ttttgcctat
      121 gaaaaattag gcatgtcagg tggaaataaa tttaaaaacc ggtatacgtt tacttatgca
      181 caagtgttag atcaacgtcc atacaagcaa gctggaaatg ctgaaatgac agttaatttt
      241 atttcaccat actctgattt agcaggtcaa gaggcagaac ttgcgcttaa gagttcccgt
      301 aacttattat tcgtagaatt gccatcagat aaagaattta aagatgatat agcaatgtat
      361 gctagaatga atctttattt aacaacgact tattcgagtg tagtaaagaa ctttgacgcg
      421 attcgttcgg aaaaatctgg tgaattacag gaactaagtg caagtatcaa cgatcaatta
      481 aatgaactgt tgaaatctgc aaaatttttc tataacggtg cagaaattca actgacaggc
      541 tccgacttta aagcaaaatt ccaatcagca cttcagaaaa tggctgaaga agtataccat
      601 aaattaaacc aaattgatgc gcctaaagaa aaagaagata tcttatattt agtgaaagaa
      661 agcaaaaatg aaattattga agatccatca aatgagcaag cagttcagga aattttcaat
      721 tttatcaatg atcaaaagag aaatcaccta acgattacga tgaaaacaat tcgtgatcgt
      781 tttgaaacaa aacaaccgta tggattcaca gaacttgatg tggaatggtg catcgctaaa
      841 cttttcatgt ctggtaaaat tgatttactt tataacaatg tacaaatttc acggatggat
      901 gatccaaata gtattgtaat gattattcaa aaacgacgag aaagtgaaaa gataaaaatt
      961 gaaccaagcg cggtgatttc gccaaaagca aaaaaattgg ttgacgaaat cggtagagaa
     1021 ctgtttcaaa gtcgtaatat tgttgatgaa aataacaatg aacatacaat ggaaaatttc
     1081 aagaaatatt taaaaggtca attagacaat ttacgctctt attttgattt acaaaacact
     1141 tatacctacc ctagtgctta tgaatatgag caaataaaaa aatatcttga agtaattgtt
     1201 caaacgtcag atgttgaaga ctttttttat acaatcaaaa aatatcgcga tgaattattc
     1261 gactgggctg aaacatatga taaaattaaa aatttccaca agcaaaatag tactcagaaa
     1321 aaaatctggg agaatgcaca agataaaatt agattaatcg aaaaaagtcg gcgtcaaact
     1381 tctagccaag aaattgatca attgattcat gagatgaaaa ccattttgaa aaaggctgat
     1441 ccttattcag atatgatgaa actaaatgag ttgattcaag aatttattga acaatataat
     1501 caactgttag agttggaaag tcaggaaata ttaaataaat taacggaaga aaaacaagca
     1561 acatttaaat tattagaagg aaaagatttt aaggatcaat ttgtaccaat agttgaccaa
     1621 gaatttcaag aactgaaagc aaaaattgaa caagcgaata ctatagatga tcttgttttc
     1681 ttatcattaa acgtagcaga acagtatttg atctttaatc aacgtttcga taatgaagaa
     1741 aaccgcagac gtcaagaaga acaagcacgt aaacaaattg aagcagcaaa cttgattcgt
     1801 gaaactgaaa aagatgaaat agactctgca gcaaaagctg aagaaatagc gatagtaaaa
     1861 cctactattc aacagaaaac agtaccaatg agaacactta acgttcatgt taaaactatt
     1921 aagtcagcag cagatattga tgagttaaca gctgaaatta atcaaaaact gaaggaacaa
     1981 cttcaagaaa atacagaaat cacgattcaa ttctagtgaa aagacaggca taaaaaggtg
     2041 cctgtctttt caaaggttac atatagaagg gaaagaaaaa tggataagaa agcgataaag
     2101 aatttcgcgg tcaatgcccg tagaaaatta attgatgaag taatgactaa agcaaaacgt
     2161 ttaggaattg atgaatcaaa aattgaagat gtgaagaaaa ttgatagcaa tttacaagaa
     2221 atcgaaaaat caggaataag aattcaagga aaagaaatag cgcaaagaaa caaattgatt
     2281 gcagaactaa aacaacgtgc ttctgtcact tcttattcag aagcttttca agagttaatc
     2341 gaagaggttg cttatacgtg gtttaaccga ttgattgcca ttcggtttat ggaggtaaat
     2401 gattatttac ctgacacgta tcgtgtatta tctagtgaaa taagtgggaa aattgagcca
     2461 gatattatta cagatattta tgatgccgat atctttgaag aattatctag taatgaacag
     2521 caacaagtca gaacttggaa aacagataat tcagcagaag caatggacaa gctgtatcaa
     2581 ttgattttta taaaaatctg taatcaattg aatgattact tacctgagct atttgaagaa
     2641 gtagaagatt atacagaact tttgtttaca gcttcttata ttaaaaaaga tggtgtaatc
     2701 gctgatcttt taagtattcc agaggatgat ttctatgttc aagctggtgg acaggtagag
     2761 attattgggt ggttgtatca gtactataat agtgagccac ataatcaagt ggtgaatatt
     2821 tataaaggga ttgtcaaaaa acaagatatt ccggcagcaa ctcaactatt taccacagat
     2881 tgggtcgttc gttatatggt tgacaatagt ttaggaaagt attggttaga tcattatccc
     2941 aatagtgaat taagaaataa gttgaaatac ttaattccag ataaatatgc ttttgaacaa
     3001 gcaacatctg taagtaattt aaaaccagaa gaactaaaaa ttatcgataa cgcaatgggg
     3061 tctgggcata ttttagttta cgccttcgat gtattgatgg aaatttatct tacgttagga
     3121 tattctgagc gtgatgctgc acgtgcaatc gttgaataca atttatatgg tcttgaaatc
     3181 gataaaagag cgtatcaatt gtcttatttt gcaattatga tgaaagggcg tcaatacgat
     3241 cgtctttttt tgaaaaaaag tctatcgccg aatttaagag agtttattga ttctcctaaa
     3301 gtgacagacg agtatcaaga gcgtattagt gaattgaaca atgataagta tagtgaacgc
     3361 tggaatgaat acaaagagtt aatttttgat caatttgaaa atgggaaaga acttggatca
     3421 atcattaagt tagtcgataa aaataatcag gcaataacta aagaatactt aggaaaagta
     3481 agagagtata tatcaaaatt caacgaattt acgaatatgg atattcttta tgggatgaag
     3541 gaaatacaag aaaaatactt gcacattctt gacgtggctg aaatgctagt tgataaatat
     3601 gtagcggtag tcactaatcc gccttatctt aataaaatgg ataagaaatt aaaaaaatat
     3661 gtaaatgata actattctga tgtcaaaaaa gatctatttt ctgtgtttat taaattgaat
     3721 agccaaatgc tgattgataa tggatatgct tcctttatga cgccatttgt ttggatgttc
     3781 atttctagct atgaaaagtt acgctcattt ttaattaatg aaagaaacat ttctagtttg
     3841 attcaaatgg agtattcagc ttttgaagaa gcaaccgttc caatttgtac gtttacgata
     3901 aaaaattcaa atgaacaaga tggaagctat ataaaattat cagattttaa aggtggaatg
     3961 gaagttcaaa aaattaaaac gttagaagca attcatcagc atggtgaatg cgattatttt
     4021 tatagtacta atcaagacaa cttcaaaaaa atcccaggaa tgccgattgc ttactgggca
     4081 agcgataacg taatcactga ttttgctcaa ggaaaattaa ctaacgatgt tgcagatgca
     4141 aaacaagggt tggcaaccgg aaataacaat aaatttttgc ggtattggtt tgaagtagaa
     4201 tttaacaaaa taaagtttga tgctaaaaca ttagaggacg ctaaaaagtc tagaaaaaa
//
LOCUS       DANKHJ010000172         4205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000172 DANKHJ010000000
VERSION     DANKHJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            994..1962
                     /locus_tag="PW113_003174"
     CDS             994..1962
                     /locus_tag="PW113_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2527409.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1979..2503
                     /locus_tag="PW113_003175"
     CDS             1979..2503
                     /locus_tag="PW113_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2527410.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2879..3160
                     /locus_tag="PW113_003176"
     CDS             2879..3160
                     /locus_tag="PW113_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527411.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3162..3578
                     /locus_tag="PW113_003177"
     CDS             3162..3578
                     /locus_tag="PW113_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527412.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1605 a          736 c          693 g         1171 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caaacacttt agtgagtttt gtagaaagta
       61 cctttaaagg tactttcaca ttacgaacga tacgtccgta ggacgtactg aaagggtggg
      121 atttatgagc gaactaatgc aaaatttggc tagcaaaaaa agttaaaaaa tatatgctaa
      181 taatcgagcg aaatgaacac tttttcaagt gagcgaatcc gccatgacaa agctgaagca
      241 gtttatttca gctttgtcat ggcgtgtgcc tagactttta aaaaagtcgg cacacgagca
      301 aatatcttta tcaagctcta atttgcgttt taagacgttt gaaacgtcta agtatataaa
      361 tatataagag taggtttaag agtgcttaaa tcgaatttta gagggcattc agacaacttt
      421 agggttgtta gggcttgccc tgaccgcctg taaggcgctt taagaacaag ataagggatt
      481 tatactaaga ctgtcttaaa acgtctcaaa cgaaggctca tagcttctca aagagttcct
      541 tatgaacatt tttagcaaag gagccggaag cgactgcgca gtataatgtg agagcagagc
      601 gatcacacct ttttaagcga ctgtagggag taagattccc agttgagaaa aatgcgcgaa
      661 tctgtttgga gaaaaaagaa gtggtttttt aggcgggaaa ttttgtcccc ctcttttata
      721 atctcttttt aaacctcttt ttaaacctct ttaccgtcgg ctatatcctt actctcccaa
      781 gggaatgacg cttttatcta catcgttttg tctgtttatc tacatcgttt tgtctgttta
      841 tctacatcgt tttgtctgtt tatctacatc gttttgtctg tttgtacatt gaattagtac
      901 atcgacgatg ttaatataag atgtagataa acaggcattt tgtatttcat aacgtgtatc
      961 tcttgtgcga gtaagaaagg agctatttta aatatgtcta atgaaatcgt aaagtatcat
     1021 catgaattaa atacaattcc gttgcgaaaa tttacaccag tcgaaatgaa tttgttcttt
     1081 tctatcgtat caagaatgcg tgatgtaggt gataaaaaag tacaatttac attcgagcaa
     1141 ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga tgatttagaa
     1201 acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga cggtttgcaa
     1261 cgtgaacgtt ttgttttatt taatcaattt aaaatcgacg gaaaatctga tattcctttt
     1321 gctgaaatac aagttcatga aaaagcctta cccttactta ataatcttga agaatgggtg
     1381 cgctattctc tccaacaatt caatgaatta gaaagcagtt attcaaaaac aatgtttcga
     1441 ttactaaaac aatacagaac caaaggtttc gcttattttt caaaagaaga ctttcatgaa
     1501 ttacttgata ttcctaaaag ttacaaccaa ggtacgattg atcaacgtgt gttcaatcca
     1561 atacgacaag aactaaccgc aatctttaaa ggtttaacaa tcaaaaagaa atacggaaaa
     1621 ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat aaaaaaatca
     1681 gacgacttta acaaacatgc cccaacaaaa gaagaacaaa acaaagcatt agaactagct
     1741 aaccaacaag ttcaatctga aaaagcacct acttcattac aagaaaaaat caaagaacaa
     1801 aagcgaaaat tggaactttt acaacagtta gaaaagttag aaagagaaca aaatacccaa
     1861 aataaagaaa ataatgcaca aaaatcagag ataaattccg atattccttc agaactaaaa
     1921 aaagacttat tagacaatct tcaaaactta ttcaaagatt gaggggtttt tagattttat
     1981 gacagaaaaa acaatcaaag aacttgctga agaattagga gttagtaaaa cagcaattaa
     2041 caaaaaagtt tctgacgaaa atcgaaaact ttggttttcg aaaatcggaa acaagtttgt
     2101 gattaatgaa accggacaaa aggctataaa atcaatgttt tttaacgaaa acgaaaactc
     2161 aaatcaacaa ctagttggcg aaaaaacgaa aaccgaaaac cacaaaaacg ccaaccaagt
     2221 tcccgaatta gaagtccttt ctctcctaga acaacagtta aatcaaaaag atatgcaact
     2281 acaagaaaaa gacgagcaaa taaaacaaat gcaaaaatta ctcgaccacc aacaacaact
     2341 caccctgcaa gccaaccaac aaatcgagaa actgcaggaa caactacagt tgacttatac
     2401 ggaagaatct tcggaaaata aatcaacgac tctttctgaa gaaacagaat cgatagaaaa
     2461 acaatcaaag gcggagaaaa aatggtggca attctggcaa taaaaccagc aatttaaatt
     2521 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
     2581 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
     2641 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
     2701 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
     2761 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
     2821 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
     2881 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
     2941 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
     3001 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
     3061 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
     3121 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
     3181 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
     3241 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
     3301 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
     3361 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
     3421 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
     3481 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     3541 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     3601 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     3661 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttggggcgct
     3721 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     3781 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     3841 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     3901 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     3961 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     4021 gtgagcgtct ctatccattg gtagaggggc tacttggtct agtcgacatt atgttaacta
     4081 gctcgataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     4141 ttatcgcata ttgtatattt taattatata attataggtg tataccactt aaatttaaca
     4201 caaag
//
LOCUS       DANKHJ010000173         4205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000173 DANKHJ010000000
VERSION     DANKHJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..538)
                     /locus_tag="PW113_003178"
     CDS             complement(<1..538)
                     /locus_tag="PW113_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2527413.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIR"
     gene            complement(538..1674)
                     /locus_tag="PW113_003179"
     CDS             complement(538..1674)
                     /locus_tag="PW113_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2527414.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1706..3157)
                     /locus_tag="PW113_003180"
     CDS             complement(1706..3157)
                     /locus_tag="PW113_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2527415.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3201..3965)
                     /locus_tag="PW113_003181"
     CDS             complement(3201..3965)
                     /locus_tag="PW113_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2527416.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(3993..>4205)
                     /locus_tag="PW113_003182"
     CDS             complement(3993..>4205)
                     /locus_tag="PW113_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2527417.1"
                     /translation="FDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLD
                     MVQARVLGVVYNGAEHSKDAGYYYGN"
BASE COUNT         1249 a          918 c          557 g         1481 t
ORIGIN      
        1 ttctaatact ttcatcataa taaaattttt tagcatccac tgcatttgga atcaaagtga
       61 atttatcatt tcgaaacaaa aaatcacctg ctacttttcc acatgaaaat tcatgtgtca
      121 aaacagacgt taacttacat cgataatatt gctttaaaag atatttaaaa tctctatcta
      181 tcgatgtatt atggctatgt gctatccgta tcggaatccc agcttttttt gcagctttta
      241 aaggtaaata actcatcgca tcaatatgag aatgaacaat ctcatattct ggatgatcct
      301 taaaaaattg ttccatccat ttaaaatatt taggataatt ttgtggataa agtctcggtg
      361 cataatagac ctttcctcca agacttatta tttcctcatc ataatcactt ttatatggac
      421 gatgtgtcaa aaaatcaaac tgaattttag ttcgatctat attcctataa tagttcatta
      481 gcatcgtctc caaccctgca cgatgcatat cattcacaca ctgtaatatt cgaatcatta
      541 tatatttccc ctatacaaat ttaatgtttc ttgtacgatt ttatcccaat tatatttact
      601 acaaataaac tcagtagctt gattcttcaa aactttaacc atttccgatt gattgcatgc
      661 ctcttctaat cttatcttta aatcacttac atccgatttc ttaaaaatca tagccttgtc
      721 ttctactact tccgtacact ctgagatatt tgaaactata caacagttcc cataactcat
      781 tgcttctaac aaacttaatg gcattccttc taaatcactt ggaagtgtat agatatacgc
      841 attactatat aattcttcta attcctgtcc ttgtacaaat ccagtaaata taattcgctc
      901 atcacctttt gctaattcct ttagttcatt tgcaaattca tctgtatcag agcttcctcc
      961 ggctattata agttttttat ctgtctgaac atctttaaat gcctcaatta gataccgaat
     1021 tcctttttcg ggaaccaatc gccccaaaaa caaaatataa gaatcttttg ttaatccata
     1081 cttttctgta atcaatcctg ctgattttat ttcaattttt ttaacgccat ttggtataaa
     1141 ccttgttttt cttccatata tatcttcaaa ataattttgg acgctttcac taagtacaat
     1201 tatttcatct gcaaatttta cagcacactt ttcccctaac atgatgtatg tactagcaag
     1261 cttgttccat ttttctcttt gatgatctag accatgaatt gttgcaacac atcttttccc
     1321 aaaaagtttt ggcaaccaca acatagcaca tggtccttct gcatgaaaat gaacaatatc
     1381 atattttccg aatgctgctc taattccacc aaatactgaa gaactcattg ctgcaattcc
     1441 ttttatattt aatgtaaaaa tagtctttaa ttttacacct ttatattctt tatttttatg
     1501 ctggtcaaat tcctttccgc taacatgatg accacttcga ttatagcaag tcacttcatg
     1561 cccaagtgca accattcgcg ttgccaattc ttctaccact atctcaattc caccttctct
     1621 tgatggaatt cttttatgac ctagcataca gatttttaat ttttttcttg ccatcacgtt
     1681 ttactccttc tatagtatct ttatcttaca tggaaccgtt tctatgaagc acagttttga
     1741 ctgtcctaaa caaaattcta aaatctaatc ccatactcca attcctgata tattctgcat
     1801 caagttttac aacctcttcg aagtctgtga tatcacttct accactcacc tgccacattc
     1861 cagtaatccc aggtttcatc gcaattctcg cacgatgatg aaaatcatac ttctcatatt
     1921 ctgacttcaa gcaaggtcta aaccccacca ggctcatttt tcccattagg acattataaa
     1981 attgtggaaa ctcatccaat gatgtagatc gaataaattg tccaattcct gtcttctttg
     2041 ttccatcagg aagtatttta ttgccaatga ttcttgggtc aaaatccatc ttaaacatga
     2101 gattattact catcttattt tctttcatca actctgcttt gcgttcttct gcatccatat
     2161 acatactacg gaacttatac atcttaaatg tcttaccatt ttgaccaata cgttcttgtg
     2221 caaagaaaat cggccctggt gatttgatgt aaataactgg tgctacgaag ataaaaataa
     2281 cacctgttgc aatacatcca ataattccag caagaatgtc aatcgttctc tttataaatg
     2341 cttgcctcat cgtcataaca ttaatactag atgtaataac agtataatca ccaactcggc
     2401 caataaattg ttttccacgt gtactatcag acacttttgt aagattcata tgtacagcaa
     2461 gtcccatttc cgaacatctg gcgattaatt ctttgggata tggaacactt tcgtcaacat
     2521 tgatgaatac ttcatctacc caattctgta aaagatattc cgctgcatta tcagcatttg
     2581 caactactgg gacacctccg atggtagttc catacatatc tctgtctact acaatcacac
     2641 cactaagttt atacatgcca taattcttct ctttaagatt acgaactaca ttttttgcaa
     2701 tcttttgaga tgttacaata actaacgata catcttcacc gtttttcatg cggtgatgca
     2761 acaacccttt ccaaagtata cgaattatat atgttaataa tccatacacc acccccatta
     2821 taaataaaga aactctggaa taatcatttc catcctgcat tgtaataagg tataaactgc
     2881 acacaagtaa cagcataaaa gattgcttta tagaagctac agcctcttta taatatcctc
     2941 tttttaaaac atttttatat gcctcaaata aaaatataat cgtaagattc tcaagttcta
     3001 taaaaattgc catgtggcga taaataggaa tcacatacgg attccagtca ccctgtcgca
     3061 ttacatatga tatataaaaa gcaagctgga cacaaaataa gtctaataaa ataaaatcaa
     3121 tatgtttaga ccaaccactt gagttttttc tatacatctc tcactctctc ctcatttcct
     3181 ttttatctac gaataaagaa ctaaaataaa ccaaacttct tcttttttac ctcttcatag
     3241 attggataat tgatctcatc cccgttgatc agatcctttg cgacttgttt catctgtact
     3301 actttttcct taccaaactc cttggcaatc tgctcatagc actcttttaa gtaaaaggtg
     3361 cgcttagaga ccccgtgtgc atcagaagca gccatctgca cgagattatg tttgaccatt
     3421 gttttggctg ttttctggat atctttgcca aatacaccaa cataacttgg cgcagtaagt
     3481 tgggccaaac atcccatatc taggaacgga agcaaatgat ttgggtcttc tcgaaaatgc
     3541 gcgttgcgtt ctggatggac gatcactggt atctttccta gttctaacaa ctcaaaaaaa
     3601 agccgttctg catataacgg cacttccagt gtcggaaatt cgatcagaag atatgtatca
     3661 tctaaatctg taaataaaat ttcatctcgg tggatgtctt cgatgagcgt tccggtaata
     3721 cgtacttctt gtccttccaa taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt
     3781 aacgaagcga ctagagaaat gacttgtgat ttttcattgc tgtattttcc attattgtgg
     3841 tgtggtgtgc ataaaatatg cgtaatgcct tgctgaatcg ctttttcagc cattgcaata
     3901 cttgcttcta agttttctgc cccgtcatcg attcctggta ggatatggca atgtagatcg
     3961 atcatttctc ttgtctcctc tcttcttatt ccttaatttc cgtagtaata accagcatct
     4021 ttgctgtgtt ctgctccgtt gtatacgaca cctaagactc gtgcttgtac catatctaac
     4081 agttcttttg ctttagtcaa tgattctttt cgtgacgtat tttcccgtac gaccaataag
     4141 gtgccatccg tttttgaaga catgatctgt gcatctgtta cggcaacgac tggcggcatg
     4201 tcgaa
//
LOCUS       DANKHJ010000174         4195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000174 DANKHJ010000000
VERSION     DANKHJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(61..2424)
                     /locus_tag="PW113_003183"
     CDS             complement(61..2424)
                     /locus_tag="PW113_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDL2527418.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="PW113_003184"
     CDS             2872..3093
                     /locus_tag="PW113_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2527419.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="PW113_003185"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="PW113_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2527420.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..>4195)
                     /locus_tag="PW113_003186"
     CDS             complement(3575..>4195)
                     /locus_tag="PW113_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2527421.1"
                     /translation="EGELKEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVG
                     AINESDVTLAAASNAIIIGFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKG
                     MLDPEFEEKITGQMIVRETFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQL
                     ASLKRFKDDVKEVKMGYECGAMIEKFNDIKVDDVIEGFIMEEVKND"
BASE COUNT         1111 a          896 c          751 g         1437 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttc
//
LOCUS       DANKHJ010000175         4094 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000175 DANKHJ010000000
VERSION     DANKHJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1420
                     /gene="pglX"
                     /locus_tag="PW113_003187"
     CDS             <1..1420
                     /gene="pglX"
                     /locus_tag="PW113_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL2527422.1"
                     /translation="KWYGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGI
                     TWSTLSSGQVSFRLQNQAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTL
                     DYHEGPMGKLPFLKVTSERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMF
                     KKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPD
                     KAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYF
                     KDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHL
                     KTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQ
                     QISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKL
                     QRDPKTGEKFQIFAKGVKEPKK"
     gene            1536..4058
                     /gene="pglZ"
                     /locus_tag="PW113_003188"
     CDS             1536..4058
                     /gene="pglZ"
                     /locus_tag="PW113_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL2527423.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
BASE COUNT         1490 a          566 c          771 g         1267 t
ORIGIN      
        1 aaaatggtat ggaaacaatg aatacgtggt taactgggag aatgatggag atgaaataaa
       61 aaattatgtt aatgaaaatg ggaagttaaa atctagacca caaaatctta attactattt
      121 taaaaaggga attacttggt cgacactttc atctggacaa gtatctttta gattgcagaa
      181 tcaggcaatt tttgaaacta aaggatctgt tctgttctca aaggatataa ctgactttga
      241 agaaatattt ggctatgtaa actctaaatt agttaatcac tttttaactt ttatttctcc
      301 caccttggat taccatgaag gaccaatggg aaaactacct tttcttaaag ttacatctga
      361 gagaaaatta aaaaaaataa gagaaaaagt ggatgaaaat atcgatatag ctagaaaaga
      421 ctataatgct cttgaaacgt catacgagtt tagaagaaac attttgttaa gttatatgtt
      481 taaaaaagat ctctcagagg catttaactg tctaagtagg cagttggtta atcaatttaa
      541 tactctaaaa tcgaatgaag aggaactgaa cgacatattc attaatcatt attctcttga
      601 aaaagaatta acaaaagagg ttcaagatga atatatctct gcaaggttac ctgataaggc
      661 gagggaaatt aaggatttta tttcttattc aataggttgt atcttcggtc gttactccct
      721 agacgaagat ggtctcatct tcgctggcgg cgaatgggat ccttcccgct atcaaaaatt
      781 taaacctgat actgataatg ttttactcat caccgatgat gagtatttca aggatagcca
      841 aattgacatt gtcaatcgtt tcattgattt cgttgagatt acttttggta aagaaaccct
      901 tgaagaaaat ctccagttta tcgctgacac tttaggtggc aaagggacaa gtcgagaaat
      961 tattcgtcgt tacttcgtac aagattttta caaagaccat ttaaaaacat actccaatcg
     1021 accaatttat tggcaattgg actcaggaaa acaaaatggc tttaaagcgt taatgtactt
     1081 gcatcgctat acaccagatc aattaggaaa agtacgtact gaatacctac acgaactaca
     1141 aaaagcgtat gacaatcgaa tcgaattgcg tcagcaacaa atttcaacaa gtgataaccc
     1201 aaaagaaaga aatgcaatga caaaagaaat tgtaaagatt cagaagcaat taaaagaagt
     1261 tcgtgagtat gacgagaagt tgggtcactt agcattaaaa cgaattgaat tagacttaga
     1321 tgatggtgta attgtcaatt atgataagtt gcagcgtgat cctaaaaccg gcgagaaatt
     1381 ccaaattttt gcaaaagggg tcaaagaacc aaaaaaatag ttatctaaga aagttgtttt
     1441 ctaacaactt tcttttttat tcttaaattg cgtgcgaaat tatccaaatt atgtcaatat
     1501 aaggggtata gaaagagaac gattaggtga gattgatgac agatataaat acgcaacaaa
     1561 ttgaagaaaa attaaatcaa cgttttccta attatggatg taggaaaatc attttttggt
     1621 ttgattcaaa tcaggatttt cttgaagaaa ttgactcatt ggtcattcaa aatgcaaaaa
     1681 tctatcgatt agaaaaggac gcacaattta aaactaagcg tttattggaa tatgaagatc
     1741 ccgaaactaa ctatttaatc tatgcgccat ttcctcgttg ggatgataaa gatgaagata
     1801 atcatttgtt gagtgtattg aattattcag aggaatttag tgcagaccgc ttagcgatta
     1861 tcatgaatga attggctatt ccacttagat ttcatcagac agttaaacag tatcgtaatt
     1921 tctttaatgc aaaggatcgc atgagaagtt ttggtgaatt tagtacgcat atgacaatta
     1981 ataaatcaga aacgattgaa ttaatgataa tggctgtttt gttgaaatcg aaaagtattc
     2041 gattaaatga tttactgcgt gtagttctaa aaagctttac agaaagtagg gagaatcctt
     2101 taagggaact tgataagtat cagttaacag ctaaattttg ggaatttatt caaatgaaat
     2161 ttggctttac ggatagccag ccaacattgg aaaagctgac gatgtgtctt tatttgaact
     2221 atttttatta tcaaattgat caagagatgc cgaagaagtt caaaggattt gaaatactac
     2281 atttgaaagc aaacattatt actttcatgg atcagtttat gaatgataca cgttacatgg
     2341 aaagctttga acagctatct aaaaaagtat atcaggctat tgatggtgat ggattattag
     2401 acagtataga aattgatgat ctaattgaag cagacgtatt ttctgagatt catgcaaaaa
     2461 tattaatgtt ttatgcagaa cgattaaagg cttctgacac tacaacgaaa attgcaggac
     2521 aaacaatttt tgatagttta aagcagcgcg aaaaaatgca tttcgcatcg gattatattt
     2581 atgagtatca attattgaaa catgcttatt atcttgtgga taaaaacaga ttgtgcttgc
     2641 cgatgaacat ggataatttg ctaactcaat atgagaagga aatgtattgg gttgatacgc
     2701 attatcggaa atttatttgg aatagtgatc atatagaaga aaagcaaaaa tatggccaat
     2761 tgtatcgatt agttgaagca aattattcga agtttcttga tcaatcagga caaatttgga
     2821 atcaaacttt tgaatttaag aatcgtccat ccattcgaaa attttataat caagctgtaa
     2881 tgaaacgctc tgttaaaact gtagttatta tttcagatgc ttttcgatat gagttaggga
     2941 tgaatttaga acgtaagtta gttagagaaa agaagtattc aacaaacatg gctagctttc
     3001 tttctgtatt accttcagtt actgaattcg gtaaagccgc cttattacca aacaatcaac
     3061 taacttacaa tgaaggaaca gaagtactga ttgatgggaa aaaaacgaat ggtttatcca
     3121 atcgaaatgc gattctccag caacgagatg caaatgctgt agctttaaat tatgatgatg
     3181 tggttaagat gaataaggct gaggtacgag aagtatttaa tggcaaatca gtcatttata
     3241 tttatcacga tcaagtggat agaacaggtg atcactcgaa tgaaaatcga gtttttgagg
     3301 cctgtgattt agcgataagc gaaatttccc gacttattaa tcgcttgcat aatgatggca
     3361 gtatctatcg ttttatagtt acggctgatc atggatttat ttataaaaga agccacgttg
     3421 aagaagtaga taagattgaa aatccatcta gaaatgaaaa agatcgtgta gaacgtcgtt
     3481 ttattcttag tgaagaagaa tacgaaataa ttggtacgaa aaattactct ttgggagatt
     3541 ctttaggaaa tgatgacact agaaacattt gtgtcccgat tacttcaagt atttttaaga
     3601 aagctggcgg gggacaaagc tacgttcatg gagggtcatc tattcaagaa atgctagtcc
     3661 ctgttctaga aattacggta gccaaaggta gcaatgcaaa ggaaccagta gcaattgaat
     3721 taatgactag caaccgcaga attacaggat tatcaaccac gttagaattc tatcaaaaaa
     3781 atgcaattag tgatgtttat gaccccgcat ctttctggct ttattttgaa gatgaatatg
     3841 gtgaacgtat ctcaaatgaa gaaatctatg ttgcggattc aaaaacagaa gatgcaaatc
     3901 agcggtttac taaatttgtt tttgaatttg taaatcgtcg atatacagtt gaagatactt
     3961 attatttagt gataaaaaat caaaaaaatg atttgaccga gcgaataaaa ttcattatag
     4021 ataatccttt tgcagaatca tttgattttg atatataaaa acgaggtatc cacaattttt
     4081 tgtggatact tttt
//
LOCUS       DANKHJ010000176         4093 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000176 DANKHJ010000000
VERSION     DANKHJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..153)
                     /locus_tag="PW113_003189"
     CDS             complement(<1..153)
                     /locus_tag="PW113_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2527424.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="PW113_003190"
     CDS             complement(475..696)
                     /locus_tag="PW113_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527425.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="PW113_003191"
     CDS             complement(794..1039)
                     /locus_tag="PW113_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527426.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="PW113_003192"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="PW113_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="PW113_003193"
     CDS             complement(2263..3264)
                     /locus_tag="PW113_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL2527427.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="PW113_003194"
     CDS             complement(3423..3689)
                     /locus_tag="PW113_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527428.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="PW113_003195"
     CDS             complement(3679..4035)
                     /locus_tag="PW113_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2527429.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1295 a          744 c          542 g         1512 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga att
//
LOCUS       DANKHJ010000177         4066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000177 DANKHJ010000000
VERSION     DANKHJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            85..1434
                     /locus_tag="PW113_003196"
     CDS             85..1434
                     /locus_tag="PW113_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2527430.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="PW113_003197"
     CDS             complement(1562..2152)
                     /locus_tag="PW113_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2527431.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="PW113_003198"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="PW113_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2527432.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DANKHJ010000178         4053 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000178 DANKHJ010000000
VERSION     DANKHJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..528
                     /gene="recO"
                     /locus_tag="PW113_003199"
     CDS             <1..528
                     /gene="recO"
                     /locus_tag="PW113_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2527433.1"
                     /translation="YLLNLVDAAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFE
                     VQILQRFGIAPIWTHCVVCGETKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFV
                     RMFSAISYDKISGIHLKEETKQAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKT
                     PEKNMPEKEIPEKEE"
     gene            912..1829
                     /gene="glyQ"
                     /locus_tag="PW113_003200"
     CDS             912..1829
                     /gene="glyQ"
                     /locus_tag="PW113_003200"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2527434.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            1831..3906
                     /locus_tag="PW113_003201"
     CDS             1831..3906
                     /locus_tag="PW113_003201"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2527435.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         1392 a          746 c          850 g         1065 t
ORIGIN      
        1 tatttgctaa atctagtaga tgcagcaatc gaagatcatc aatatgaccc gcatttattc
       61 caatttacgc aacaagctct gcagcggatg gatcaaggag atgatgcaga aatcatcacg
      121 aatatctttg aagttcaaat actgcaacgc ttcggaatcg cgccaatttg gacgcactgt
      181 gtcgtatgcg gggaaacaaa aggaaaattt gactattctt ccaaatctgg cggtgtgata
      241 tgcgagaaac actggccaat ggacgaacac cgttatcatg cagatcctcg cgctgtttat
      301 ttcgttcgaa tgttttcggc tatttcttat gataagatca gtggaatcca cttgaaagaa
      361 gaaacgaagc aggcaatccg tgacctcatt gaccagttgt atgatgaata tgtcggtatt
      421 catctaaaaa gcaaaaagtt cattgatcaa atgaagtctt gggcacacgt attaaagacg
      481 ccagagaaaa atatgcctga aaaagagata cctgaaaaag aagaataaat gaaagataca
      541 acaaaaaaat caaaactatt tgacaaatac agggacttcc gtctattatt aaccgtagat
      601 taaatagatc gttcactgag aggatctatt atacaaacca gaaaactgca aaatgaatac
      661 gtacattgac caagatcagt aaaaaacacg tggtttagcg agttcgggag agtggaagcc
      721 ggacccaaac gatttttgaa gaggcacttg cgagtacgat aaatgaagct gccgaatctt
      781 tattcggaag tagggtggaa ccgcgaaatt attcgtccct atgtctgtaa tcagacatag
      841 ggactttttg tcgttttctt tcctgtattc ttattgacag caacgcttaa aaattgaaag
      901 gggcaaaaca gatgagtaaa aaattgaccg tccaagatat gattttaaca ttacaaaaat
      961 tttggtcatc aaacggctgc atgctgatgc aagcttatga tacagaaaaa ggagcaggta
     1021 cgatgagccc atatacattc ttacgggcaa tcggaccgga accatggaat gctgcttatg
     1081 tagagccatc ccgccgacca gctgacgggc gttatggaga aaacccaaac cgtttgtatc
     1141 agcatcatca atttcaagtc gtaatgaaac cttcacctga aaatatccaa gagctttatt
     1201 tagaaagctt gcgtctttta ggaattgatc cattagaaca cgatattcgc tttgttgaag
     1261 acaactggga gaacccatcg atgggttgtg ccggtgtggg ctgggaagta tggcttgatg
     1321 ggatggagat cacacaattc acatacttcc agcaagtcgg aggactacct tgtaaaccag
     1381 ttacttctga gatcacttat ggactagaac gtcttgcttc ttatatccaa gaagttgaaa
     1441 gtgtttatga tctggaatgg gctgatggcg tgaaatatgg tgagatcttc aaacaaccag
     1501 aatatgaaca ttctaaatat tcttttgaag taagcgacca agacctgcta ctaagcaatt
     1561 ttgatcgttt tgaaaaagaa gctaaacgct gtatcgatga aaatcttgtt catccagcgt
     1621 atgattatat tttgaaatgt agccatacat tcaatttatt agatgctcgc ggtgcagtat
     1681 ccgtaacaga gcgtgcaggt tatctttccc gtatccgcaa catggctcgt gctgtagcca
     1741 aaatctttgt ggcagaacgc gaaaaattag gattcccttt attgaataaa aacacagcaa
     1801 atccagaaaa cgtgaaggag gctgaataag atggcaaaag acttattact tgaaatcggg
     1861 ctggaagaaa tacctgctca tgtagtcaca ccaagcatga aacaattaga agaaaaaaca
     1921 gcaaaatttt tagatgaaaa ccaattgact tatgatacga tcgaaacgtt ttctacaccg
     1981 cgccgcttag caatcaaagt aacaaacatt ccagaacgtc aagcagacag cgaagaagaa
     2041 gtcaaaggac cagccaaaaa aattgctttg gacgcagatg gaaactggac gaaagcagct
     2101 gaaggatttg tccgcggaca aggtctgacg acagatgcta tcacttttag agaattaaaa
     2161 ggcgtggaat atgtctatgt aacgaaatac actcacggca aagaagcaaa agaagtattg
     2221 acaggtttag cagatgtcat taaaagcctc actttcccag taacgatgca ctgggcagaa
     2281 tacgattttg aatatattcg ccctattcac tggatcgtgg cattgttaga tgacgaaata
     2341 atcccgtttg agatcttgga tgtcaaaaca gacagagctt caagaggtca tcgtttctta
     2401 ggcgaagatg tgacatttac gcatgcaaaa caatacgaag aaaaattgaa tgaacaattt
     2461 gttattgctt caccgcaaaa aagaaaagaa atgatcatca cacaagcaaa cgaattagca
     2521 gaaaaaaatc actggatcat tgatctagat gaagatttac tagaagaagt caacaattta
     2581 gtcgaatatc caactgtctt ttctggcgac ttcgaagaaa aatatctatc tgttcctgaa
     2641 gaagtactag tcacttctat gaaagaacat caacgttatt tcgaagtacg gaacgatcaa
     2701 ggtatgttgc ttcctcattt tatttctgct cgtaatggcg atcgggtaaa attggaaaat
     2761 gtggtacgag gcaatcaaaa agtattgact gctcgtttag aagatgcgga attcttttat
     2821 aatgaagaca agaaagcttc aatcgaccaa tacgttgaaa aattaaaaac tgttactttc
     2881 catgaaaaaa tcggtagcat ctatgagaaa atgcaacggg tacaagtcat cgctcgcatc
     2941 ataggcgaac aagtaggact atctgaggaa gaattgatgg atctgcaacg cgctgctgaa
     3001 atctataaat ttgatctagt aaccaacatg gtaggagaat tccctgaact acaagggatc
     3061 atgggcgaaa aatatgctct gcttaaaggc gagaaaccag ccgtagcaac agcgatccgt
     3121 gaacattaca tgcctatatc cagtgaagga gatttacctg agactacagt aggttctgtt
     3181 ttagcgattg ctgataaatt agatagtatc ttttctttct ttgcagttgg gatgatccca
     3241 agtggctcaa acgatccata tgctttgcgc cgtcaagcat atggtgtcgt tcgaatcatc
     3301 gaatcgaaaa aatgggcctt tcctttgtct gttctgcaag aaacgattag tgaagttatc
     3361 agtaaagata cggatcgttt tggcattggc ttgtcagcgg gacaacaaca ggtgatcgac
     3421 tttatcaaag gccgtctgcg ccaattattg acaacgaaga atatccgtca tgatgtcatc
     3481 gaagctgttt tgaatgcaga acaaaaagat ctgacaaaag tatttgcagc agcacaattg
     3541 ttcaagcagc atcttgcaga tgaagacttc aaaccatcga tggaagcttt gacacgtgtc
     3601 gtgaacttag cgaaaaaagc ggagcttgaa aaacaatcag aagtagaccc tgaactgttc
     3661 gaaaacgaag cggagaaaga actgcacaaa gcagtagagg caatgaaaac aagctttgct
     3721 gaccaaacga tcaacgaaaa ttatgatcag cttgtaagcc ttcgcccact gatcgaaaat
     3781 tattttgatc agacgatggt catggcagaa gaaccagcag tacgtaagaa ccgattaacg
     3841 caattaagtc aaattgctgc catggctttg tcactagcaa gtttagatca aatcatcaca
     3901 aaataatttc taatatgtaa ataaaccaga gaacaggcac aaaataggct atttctctgg
     3961 tttatttgtt ataaatataa ataactatta ttaattcaat gttaattttt ttgttatttt
     4021 attttttgtg taacttttaa aaacacctat gaa
//
LOCUS       DANKHJ010000179         4030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000179 DANKHJ010000000
VERSION     DANKHJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            958..1926
                     /locus_tag="PW113_003202"
     CDS             958..1926
                     /locus_tag="PW113_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2527436.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1936..2424
                     /locus_tag="PW113_003203"
     CDS             1936..2424
                     /locus_tag="PW113_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2527437.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            2720..3001
                     /locus_tag="PW113_003204"
     CDS             2720..3001
                     /locus_tag="PW113_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527438.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            3003..3419
                     /locus_tag="PW113_003205"
     CDS             3003..3419
                     /locus_tag="PW113_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527439.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1502 a          702 c          671 g         1155 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgtgtttt gtagaaaggg
       61 accttatagt cactttcaca ttacaaacga tacgtccata ggacgtatca aaagggtggg
      121 acttatgagc aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg
      181 atcagagtca aaataaatca gttttcgagt gagcgaatcc aaatttgcaa ctctgaagct
      241 ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata
      301 gggctctaat ttgcgtttta aggcgtttta aacgttctgg tatgtaatta taccaaagcg
      361 actttaaaac gccttaaaac gaattttaga ggttctgtag aacgttttag ggttgttagg
      421 gcttgccctg accgcctgta aggcgcttta aaaacaagat aagggatttt atctagactt
      481 gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa ggactcctta cgaactgttt
      541 ttaagcaaag gagcctttag gcgactgcgc agtttaatgt gagagcgtag cgatcacacc
      601 tttaaagcga ccgtagggag cagattccca gtttaggaaa tttcgcgaac ctgtttggag
      661 aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc ctcttttata atctctttta
      721 taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt actctcccaa ggtgtgcaga
      781 atcttttagg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
      841 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttaatta gggggttgac
      901 ccccattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
      961 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     1021 tcagtagaaa tgaatttatt cttctctata atatctagaa tgcgtgatgt aggcgataaa
     1081 aaagtacaat ttacattcga acaattaaaa gatttaagca attataaagc aactgcaaat
     1141 gttcgattca tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt
     1201 agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt tatttaatca atttaaaatc
     1261 gatggaaaag ctgatattcc ttttgctgaa atacaagtcc atgagaaagc cttaccctta
     1321 cttaataatc ttgaagaatg ggttcgatat tctctccaac aattcaatga attagaaagc
     1381 agttattcca aaaccatgtt tagattatta aaacaataca gaaccaaagg aattgccaat
     1441 ttttcaaaag aagactttca tgaattactt gatatcccta aaagttatag agaaagtgat
     1501 ataaataaaa aaattataaa gcctattcgc caagaactaa ccgcaatctt caaaggtttg
     1561 acaatccaaa agagatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca
     1621 tttaaacctg agataaaaaa atcagacgac tttaacaaac atgctccaac caaagaagaa
     1681 caaaataaag ctttggaact agctaatcaa caggttcaat ctgaaaaaga acctacttca
     1741 ttacaagaaa aaatcaaaga acaaaaacga aaattagaac ttttacaaca gttagcagaa
     1801 ttagaaagaa aacaaaatac cccaagtaag aaaattaaca aacaaatagc agataacaat
     1861 tccgatatta ccccagaatt taaagaaaac ttactagata atctccaaaa cttatttaaa
     1921 gaataagggg tttttatgac agataaaact atcaaagaat tagcagatat tctaggtgtt
     1981 tcaaaagata aagtgaagta tcaactgagg aaattaccta gtaattttac ctacaaaaaa
     2041 ggaaatatta cctacctgaa aaaagaagca gtaacaacaa ttacaaaggt tttatcaggt
     2101 aatgaatcgg gtaacttacc tagtgaatta cccgattcat tacctaccga ttacctactc
     2161 aaggagattg aaactaagaa taatcatatt gaagaaaaag ataggcaaat tgaaaaactt
     2221 caaatagctt tagaaacgca gcaaaaatta ctcgaccagc aacaacaact caccctacaa
     2281 gccaatcaac aaatcaaaaa acttcacgaa caactccagc taacctatgc agaagaatct
     2341 tcagaaaaag aacccctttc tgttactgaa gaaactcaaa aaggacaaaa caaggagaaa
     2401 aaatggtggc aattctggca ataacccaac aatttttatt cataaaaatt gttgggttac
     2461 tttctttttt tgcttttaca agcatagcga catgatctca cgtcgctatt ttttaaagag
     2521 cataaggaaa cgcggtatca ctttcttacg aaaggtgcta ggtaggctgt gcctaccacg
     2581 tttctaagaa aaaaatttgc tatcactttt tcgtcattgt ggatagctta tgtatagtgt
     2641 ttcggcaaaa aacagctata caaatactat atttgtatga tatacataaa ttatacacaa
     2701 atgcgaaagg aattcttcta tggacatttt agttcggaat attgatccaa aagttatttc
     2761 agaaatagat agacgttgcc aagaactcac aacaaaaact ggcaaaaaat ggagccgtaa
     2821 tgcgtaccta aaagttctaa tcgaaaacga ttttgaccag gcacttaggc attacaaaaa
     2881 agatcaattc gatttattac tcgaaaaatt tatcgatata caagcaaaaa atactgaaac
     2941 attgaacgac tatgttcata caaacaacca actcattgaa acacttatcg gaggaatata
     3001 acatgagaaa aacttctgtt caaaaacacg ttacccttta tcccagtcac attgacgttt
     3061 tagattccat catcgaaaaa aatccagata tcaataacta ctcggaagcc attcgttttc
     3121 tatgtatgag ttatgaacct aatactaata cgacaactac cagcaaccaa gcaaaactaa
     3181 atgcgatggg aaaagaaatt tctattatgt ccgaaatcat tgcagaattt gctgatttat
     3241 atctaaaaga tactggtgtt cttatcggta caaattctaa agtatatcaa gaagtgaaag
     3301 aaaaaattga aaatcaactc aatccctccc gacgaaatca acccaaaaat cgtcaaaaag
     3361 aaaccaatca aacattcact tctagaaatt ttgacccaac aaactttcta aaaagatgaa
     3421 aaagaaaccg aaaatttttt ctatttctca gtttcttttt tagggggcag tcttccagca
     3481 agatttgcaa gttaccggta acttgcacaa aaaaagaaaa atccgcttgt gcgaatcctt
     3541 ctttttgcat tttggggcgc tgccccaatc tccaaatatc ggttatttaa tagcgtggta
     3601 actcaaaaaa atagaacgta ctctgaaata aaaaaagact gctctaacga gcagccaata
     3661 aaaaataatg agcgtttatt cttgaaaaag cagcgcgctt atgaagcgct gccaaaaatt
     3721 taccatattt tatgaaaaat aacaagtttc taaaaaggaa tcggttaaga ttcacaaaga
     3781 atcgtaaaca gacaatagcg gaaaatcaag cttccgaacg taccttttag ctagaccaat
     3841 gaaaaatcac aaagtgagcg gctggatccc ttggtacagg cgttacttgg tagtatacac
     3901 attatgttaa ctaacgctat aaataacgat tatgttccca aaacaatatt ttatgaacta
     3961 taacaaaaat ttgttatcgc atattgtata ttttaattat aggtgtatac cacttaaatt
     4021 taacacaaag
//
LOCUS       DANKHJ010000180         3877 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000180 DANKHJ010000000
VERSION     DANKHJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(717..3866)
                     /locus_tag="PW113_003206"
     CDS             complement(717..3866)
                     /locus_tag="PW113_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2527440.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DANKHJ010000181         3846 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000181 DANKHJ010000000
VERSION     DANKHJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..266
                     /locus_tag="PW113_003207"
     CDS             <1..266
                     /locus_tag="PW113_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2527441.1"
                     /translation="EEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLARELTDNSLPK
                     IGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            464..1594
                     /gene="dnaN"
                     /locus_tag="PW113_003208"
     CDS             464..1594
                     /gene="dnaN"
                     /locus_tag="PW113_003208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2527442.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            1823..2065
                     /gene="yaaA"
                     /locus_tag="PW113_003209"
     CDS             1823..2065
                     /gene="yaaA"
                     /locus_tag="PW113_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2527443.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            2052..3176
                     /gene="recF"
                     /locus_tag="PW113_003210"
     CDS             2052..3176
                     /gene="recF"
                     /locus_tag="PW113_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2527444.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            3173..>3846
                     /locus_tag="PW113_003211"
     CDS             3173..>3846
                     /locus_tag="PW113_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2527445.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVIN"
BASE COUNT         1314 a          667 c          764 g         1101 t
ORIGIN      
        1 aagaagaagt ggcaaagtat tatcacatcc aattgaaaga tctcaaagga aagaaacgcg
       61 tcaaatctat tgttgttcct agacaaatcg ctatgtattt ggcacgtgag ctcacagata
      121 attctttacc gaaaatcgga gctgaattcg gtggcaagga tcatacaacg gttatccatg
      181 ctcatgaaaa aatccagcag ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag
      241 aaatcaaaaa ctttttacta aattaatttg tcgattctat caactgaaaa tagcatgtgg
      301 ataaaagaaa aaaacgttca aaagttatac acactttttc cacagctgtt ttttcgtttc
      361 tctcttacgt tttttgagtt ttccacagaa tcaacatgcc ctattacttt tattattctt
      421 tttaactaat aataaataaa caaacattca aaggagatac atcatgaaag ttactttaaa
      481 ccgagctagc tttatgcagg aattgcaaac tgttcaacga gctatttcaa gcaaaaccac
      541 gatccctatt ttgacaggtg taaaaatcac actgacacaa gaaggtttga ctttgacggg
      601 gagcaacgct gatatatcaa ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat
      661 gcaaatcgaa tctactggtt ccattgtttt acaagcacgt ttctttagcg aaatcattcg
      721 gagacttcct gaagaaacat ttactttaga agttttagaa aataaacaag tagcgatcac
      781 ttctggaaaa gcgaatttta tcgtaaatgg attagatgca gataactatc ctcatcttcc
      841 tgttgtcgaa agccataacc agatgaaatt acctgtacac gtattgacta aactaatcaa
      901 cgaaacagtt tttgctgtct cacaacatga gagtcgtcca atcttgacag gtgtgcattt
      961 cattttatct gataattctt tattagctgt agctactgat tctcaccgtc taagtcaacg
     1021 cgtgattcca gtagaacaag cggctgatca ttttgatatt gttattcctg gaaaaagttt
     1081 gatcgaatta tctcgctcat taacaaatga agaagaaatc gtcgaaatca gcattatgga
     1141 aaaccaagtg ttgttcaaaa cagaaacgat gtatttctat tcccgtttgc tagaaggaaa
     1201 ctatccagat accaatcgtt tgattccttc aagttttaat acagaggttg aattttctgt
     1261 tcctagcttt ttagcggcga tcgaacgggc ttctttactt tctcatgaag gccgtaacaa
     1321 catcgttcgt ttatctattc gtccagatgc ggttgtttta tacggaaatt cgccagaaat
     1381 cgggaaagta gaagaaagct tgagttatac agcaagcagt ggcgatccat tagatatttc
     1441 tttcaatccg gattatatga aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt
     1501 gaaatttatc tctgctattc gtccatttac attggaacca acggaagatg gcgttcaatt
     1561 tattcagctg attactcctg tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat
     1621 ttaattgaaa agaaatagaa aaaataaaat tttaaacaga aggccctaaa atctctttta
     1681 aggctttctg tttatttttg tttaaatcac cttttttaga ttaaatcgtc ttatatggaa
     1741 aaatatatgg ataaatttgt tttattgcga aaaaaaaggt ataatagagt atacttttaa
     1801 taatgaaaat gagggatggg cattgaaaca acaagtcatt ctaaatacgg aattcatgac
     1861 gttgggccaa atgttgaaag aagtcacagt tatcggtagt ggcggacaag caaaatggta
     1921 tttggcagaa aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact
     1981 ttatcccggc atgatggttg aagttccaga agtaggaact ttttttatgg taaaaaaagg
     2041 aaacgacgat aatgaggctg aataagctgt atttaaaaaa ttatcgaaat tacgaagagc
     2101 tgaacatcga attttccaag aacctcgtca tctttttagg agaaaatgcg caaggaaaaa
     2161 cgaatctttt ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg acaagtagtg
     2221 agcaggaatt gattggctgg actgacgatc aggcgatgat tcaaggagag atcacaaaag
     2281 gttcatccac tcttccatta gaaatcttat tatctaaaaa aggccgaaaa accaagatca
     2341 accatatcga acagaaaaag ttaagtactt acgtgggaca aatgaatgtc attttatttg
     2401 caccagaaga tctttcttta gtaaaaggca gcccgcagat caggcggaag tttttagata
     2461 tggaattagg ccagatcaat ccaatctatc tttatgatct tgtccaatac caagcaattt
     2521 tgaaacaaag aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt
     2581 atttggatat tttgtccgaa caactagcag cgtttggcag caaagtattg aaagcaagag
     2641 cacagttcgt caagcgattg gagttttggg ctaattcttt gcatcagcaa atcacccatc
     2701 aaaaagagca gctggaaatc gagtatctca ccgcagtcga ttctttagaa acacatacgc
     2761 aagagcaaat ccaagaacaa tttctcgcac ttttgaacca aaataaaaag aaagaccttt
     2821 ttcggggaac aacgacagtt ggtcctcatc gtgacgatct ttcctttttt attaatcaga
     2881 aaaatgtgca gacttatgga tcgcaaggac agcaaagaac aactgcacta agtgtcaaat
     2941 tagcagaaat cgatttgatt aaagaagaga caggggaata tccaatcctt ttgttagatg
     3001 atgtgatgag cgagctggat gataatcgtc aactccatct gctagaaaca atcgaaggaa
     3061 aagttcaaac gttcttaaca acaacgacac ttgaccatgt aaaaaataaa atgactgttg
     3121 aacctgagat tttttatgtt caacaaggga aactaaaagg agaagaaact gaatgacaga
     3181 agaaagaagt ttagtagaac gcgctaaaga gtatgatgcc agtcagattc aagtacttga
     3241 aggacttgaa gccgttcgta aacgacctgg catgtatatc ggttcgacta gttctgaggg
     3301 tctgcatcac cttgtttggg aaattgtcga taattcgatc gatgaagtat tagcaggttt
     3361 tgcaacgaaa atccatgtga ttattgaaaa agacaacagt atcaccgtta ttgacgacgg
     3421 tcgggggata cctgtagata tccaggcaaa aactggacgt cctgcagttg aaacggtctt
     3481 tactgtttta catgctggag gaaaattcgg cggtggtggg tacaaagttt ctggtggact
     3541 tcacggagta ggatcttctg ttgtaaacgc gctttctaca cagcttgatg tcaaagtata
     3601 caaagaagga aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa
     3661 gattatcgaa caaacggatc gccatggaac aacggttcac tttacgccag accctgaaat
     3721 ttttacagag acaactgagt ttgatttttc aaaattggct acccggatcc gtgaacttgc
     3781 tttcttgaac cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag aaccagtcat
     3841 caatga
//
LOCUS       DANKHJ010000182         3718 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000182 DANKHJ010000000
VERSION     DANKHJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..353)
                     /locus_tag="PW113_003212"
     CDS             complement(<1..353)
                     /locus_tag="PW113_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527446.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="PW113_003213"
     CDS             complement(613..1161)
                     /locus_tag="PW113_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527447.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="PW113_003214"
     CDS             complement(1162..2019)
                     /locus_tag="PW113_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2527448.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="PW113_003215"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="PW113_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="PW113_003216"
     CDS             complement(2581..2910)
                     /locus_tag="PW113_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2527449.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="PW113_003217"
     CDS             complement(2987..3259)
                     /locus_tag="PW113_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2527450.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="PW113_003218"
     CDS             complement(3259..3522)
                     /locus_tag="PW113_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2527451.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DANKHJ010000183         3714 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000183 DANKHJ010000000
VERSION     DANKHJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            72..155
                     /locus_tag="PW113_003219"
     CDS             72..155
                     /locus_tag="PW113_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2527452.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="PW113_003220"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="PW113_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2527453.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="PW113_003221"
     CDS             complement(1280..2143)
                     /locus_tag="PW113_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2527454.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="PW113_003222"
     CDS             complement(2145..2417)
                     /locus_tag="PW113_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2527455.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="PW113_003223"
     CDS             complement(2434..2643)
                     /locus_tag="PW113_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2527456.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="PW113_003224"
     CDS             complement(2721..3617)
                     /locus_tag="PW113_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2527457.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1142 a          652 c          575 g         1345 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttt
//
LOCUS       DANKHJ010000184         3701 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000184 DANKHJ010000000
VERSION     DANKHJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..663
                     /locus_tag="PW113_003225"
     CDS             <1..663
                     /locus_tag="PW113_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HDL2527458.1"
                     /translation="YESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDEIMTVH
                     YEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMKLAQQI
                     PSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVKALFEA
                     DFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAEAIDYV
                     TV"
     gene            complement(858..1304)
                     /locus_tag="PW113_003226"
     CDS             complement(858..1304)
                     /locus_tag="PW113_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2527459.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            1532..1681
                     /gene="rpmG"
                     /locus_tag="PW113_003227"
     CDS             1532..1681
                     /gene="rpmG"
                     /locus_tag="PW113_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2527460.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            1769..2287
                     /locus_tag="PW113_003228"
     CDS             1769..2287
                     /locus_tag="PW113_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2527461.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(2364..3320)
                     /locus_tag="PW113_003229"
     CDS             complement(2364..3320)
                     /locus_tag="PW113_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2527462.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            3486..>3701
                     /locus_tag="PW113_003230"
     CDS             3486..>3701
                     /locus_tag="PW113_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527463.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSS"
BASE COUNT         1144 a          668 c          707 g         1182 t
ORIGIN      
        1 tatgaatcac ttgctcctgc aaaagaatat tttatcggtc atgctcgagt gttcgaagaa
       61 tcagaagcat cagaagtgtt aacaccaaaa tatcaaacag caccatctgt ggatgaaatc
      121 atgactgttc attacgagaa aacagaagga atcaaagatg aagtcaataa gatgcaatat
      181 gtcgatcttc atcaatggat gccaaaagat atcttgttaa aggcagataa actttctatg
      241 gcaagttctt tggaagtccg agtaccttta ttggatatcg aagtcatgaa attggctcaa
      301 caaataccaa gtaagttttt attgaatcaa aacaacacaa aagatatttt cagacaagct
      361 gcgaacaaac atcttccgga agaatggtcg aatcgtgtga aattaggatt ccctgtgcca
      421 atcaaagctt ggttgaaaga agaacacggc tacgaacaag tcaaagcatt attcgaagca
      481 gattttgcaa aagaattttt tgatcaagaa aaaatcatga agttgctaga tgaccatcac
      541 gaaggacgta aagaagagca aagaaaaatc tggacaatct tcagtttctt gacatggtat
      601 aaagtctact ttgtagatga aacgattccg caagcagaag caatcgacta tgtgactgtt
      661 tgatgaaaat caaatatcaa taaaaaagag gttttctcct accgtttaac actgagtgat
      721 aagaaacaat ttcaaaaaac agctcttccc gttttataga tattatgact tatggttcgg
      781 agttggcaga ggaacaccat tgtttcgaac ataagggggt tgtgacacga ttttcgtcac
      841 aaccccttta catatgttca ataatcgatt tctttttctt ttgtcccatc ttttttataa
      901 atgaacaaag ttgattcttt gttttgtgct acttgctttc ctttttcgat tgcttctttt
      961 tttgtatcaa aatggttgct tgcctttttt gcccctttag atttcacgat ccattgttct
     1021 tcctcatatt ctacgatcac atctgaatca agcagttttc cagcacgagg attcgtatcg
     1081 tgttcatccg tcttgcttgg atttttttct ttttcgaaag ttcgtttttc tgattcacta
     1141 gcatcttcat accatttttt tgcttgatca atggcaattg ggatcgcacg gttatcaggg
     1201 tacccttcat ctaataatgc atttgcgatg tcgattgctt tttttcttgt caatttatct
     1261 aagtttttca ttgatgcagg aaagtccttc atgttccatg gcatattagt atcccccttt
     1321 attaaaaaac ataatctata taaacaagtg taagggtatt tatacaaaaa gaaaaagatt
     1381 ttgtttgaga agtaaaaccg ttgtatttat cgtcaagtag ttttacttaa caaaatcgtg
     1441 aaaaacacta gcaatctaga aaaaatcatg gtaagataat caagtctgag agtcatctta
     1501 gaggattctt gtaaaaatgg gagggaaaaa catgcgcgta aacatcactt tagagtgtac
     1561 ttcttgtaaa gaacgtaact atctaacaag caaaaacaaa cgtaacaatc ctgatcgttt
     1621 ggaaaaacaa aaatattgcc cacgcgaacg caaagttact ttgcaccgtg aaacaaaata
     1681 atgtttttaa agttctcaat tccttgctgg tcaaagaatt gagacttttt ttatctcaaa
     1741 acatctaaaa tcataaggag cggataatat gtttatttcc atgtgggcac aagataaaaa
     1801 tggtctgatc ggcaaaaatg gtttgttacc atggcgattg ccaaatgata tgcgtttctt
     1861 tcgcgaacat acaatggatc gaattttagt gatgggaaga aagacctatg aaggaatggg
     1921 ggatctttca cttccttatc gccatatcat cgtgttgaca acgcaaccaa attttcgcac
     1981 gaaagaaaat gcggaagtaa tgcattcgat cgatgaactt ttgtcgtata gtcaaacgat
     2041 ctcagaagat atctatgttt cgggtggtag tcggattttt caagaacttc ttccttacac
     2101 tggtcggatc tggcgaacat tgatcgatag cacgtttgaa ggcgatacgt atattgggaa
     2161 tattgatttt tcagacttcg cccttataga ggagtatgag gggataacgg atcaagaaaa
     2221 tctatatgcc catcgttttc aaaaatggga aagagtaaaa aatagggttg cttcaaaaga
     2281 agaataaaat ctatgcgtct agtttgttaa taagaaaaaa agcatctcac gactaatcgt
     2341 gaagattctt tttttagtta aaatcaattt agtttttctt tgatatcatt gaccatttcc
     2401 tgactggcaa taggtccgtt atatagccaa ctagattcag gtccgaattg cagtacatga
     2461 ttgtctgtca ctgctttaag attcttccag cttggttcat tgaacatggc agcatctttg
     2521 tcactgttca caaggatgat gtagtcagca tctaattcag ctagcttttc agaggaaaca
     2581 gcagaccagt cagaagtggc ttttttggag acctcttcca ctagatcagg aacaccaaat
     2641 tccagatcgt cgtatacgat tcttccactt gaccgatttt tgctgaccat aaaggctgag
     2701 ttgttggtca cccacaacac tgcagctgtc ttgttctcga ttttatctgc tagttcttct
     2761 cgagttttct tgatggattt ttgataatcg gaaatgattt tttctgcctc tttttctttt
     2821 gccaaagctt tgccgacatc ttttagttgt tcttcccatg tgacatctgt tccgtttttt
     2881 acaacataag ttggcgcgat tttgctatat tgttcgtatt tcccgccttc gacagtagcg
     2941 gaagaagaaa tcaacagcaa atcaggctca aagcttaaca ctttctcata aggaaggtca
     3001 taagaaatcg ttgggacatc ctttaaatca tcttgcagat aatcttgaat ggaaccactg
     3061 ccaacagtcc actgggcgat aggtttttcc cctagtgcaa tcagatagtc ttctaagtaa
     3121 ctgccgatga tccgtttggg cgcgtttgga atctctactt tatgtccaag tgtatctgtg
     3181 actgtatgag tactattgga tgaagtcgtc gaattggcag ttgtagctgt ttctttatct
     3241 gaagaggggg agcaagcgct cagtactcca gccagcagca atatgctaga taatgtgaat
     3301 acttttgttt ttaatttcat ttctttcctc ctaacataat tgagaatctt tctcattggt
     3361 tattattcct gatatttcga acgctgtcaa ccgcttcatt tggctataga tatgaaagta
     3421 gcgcttgacg aaatgttgaa acagcgtcat aattgagaat gattttcagc gaaaggatgg
     3481 gtgaaatgac gagattaaga gcagctgaca ttgcagccgg ctacgaaaac aaaaaaatca
     3541 ttgaaaacat gtctgttacg attcctgaag ggaaaatcac tagtttgatt gggccaaatg
     3601 gaagtggaaa atctacttta ttgaaaagtt ttacccgtat actacctgtg aaatcaggaa
     3661 agatcattgt caataactct gatatctcta catacagttc a
//
LOCUS       DANKHJ010000185         3656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000185 DANKHJ010000000
VERSION     DANKHJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..2124
                     /locus_tag="PW113_003231"
     CDS             <1..2124
                     /locus_tag="PW113_003231"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2527464.1"
                     /translation="TRDRAGYEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVN
                     KFGFIETPYRRVDRETGRVTDQIDYLTADIEDHYIVAQANSPLNEDGTFAQDVVMARA
                     QSENLEVSIDKVDYMDVSPKQVVAVATACIPFLENDDSNRALMGANMQRQAVPLINPQ
                     APWVGTGMEYKSAHDSGAALLCKHDGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNS
                     GTSYNQRPIVHLGEKVEKGDTLADGPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMS
                     RRLVKDDVYTSIHIEEYESEARDTKLGPEEITREIPNVGEDALKDLDEMGIIRIGAEV
                     QDGDLLVGKVTPKGVTELSAEERLLHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFT
                     REAGDELSPGVNMLVRVYIVQKRKIHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTP
                     IDIMLNPLGVPSRMNIGQVLELHLGMAARQLGIHVATPVFDGASDEDVWETVREAGMA
                     SDAKTVLYDGRTGEPFDGRVSVGVMYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPE
                     SFRVLVKELQSLGLDMRVLDIEETEIELRDMDDEDDDLITVDALTKFAEQQTAKELEK
                     KAAEQVEDEKDDVIQNFETAEDNLD"
     gene            2182..>3656
                     /locus_tag="PW113_003232"
     CDS             2182..>3656
                     /locus_tag="PW113_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2527465.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDG"
BASE COUNT         1132 a          726 c          870 g          928 t
ORIGIN      
        1 actcgtgacc gcgccggtta tgaagttcgt gacgttcact actcccacta tggccgtatg
       61 tgtccgatcg aaactcctga aggcccaaac atcgggttga tcaatagttt gtccagttat
      121 gcgaaagtaa ataaattcgg atttatcgaa acaccttatc gtcgtgtaga ccgtgaaaca
      181 ggtcgtgtta ctgaccaaat cgactacttg acagctgata tcgaagacca ttacatcgta
      241 gcccaagcaa acagcccatt gaatgaagat ggaacatttg ctcaagacgt tgttatggct
      301 cgtgctcaaa gtgaaaactt agaagtatct attgataaag tagactacat ggacgtatca
      361 cctaaacaag tagttgccgt tgcgacagca tgtattcctt tcttggaaaa cgatgactcc
      421 aaccgtgcct tgatgggtgc caacatgcag cgtcaagcag tgccgttgat caatcctcaa
      481 gcaccatggg ttggtactgg tatggaatac aaatcagcgc atgactcagg agctgctttg
      541 ttatgtaaac atgacggtgt cgttgaatac gttgatgctt ctgaaatccg cgtacgccgc
      601 gacaacggtg cattagataa atacgatgta acaaaattcc gtcgttcaaa ctccggaaca
      661 agctacaacc aacgtccaat cgttcatttg ggtgagaaag tcgaaaaagg ggatactctg
      721 gcagacggac catctatgga acaaggcgaa atggctctag gccaaaacgt tttagttggt
      781 ttcatgactt gggaagggta caactatgaa gatgcgatca tcatgagccg tcgtttggta
      841 aaagatgacg tatacacttc tatccatatt gaagaatatg aatcagaagc ccgcgataca
      901 aaattaggac ctgaagaaat cacacgcgaa atccctaatg tcggggaaga cgcattaaaa
      961 gatttagacg aaatgggtat catccgtatt ggtgcagaag tccaagatgg cgatttgctt
     1021 gtagggaaag tcacgccaaa aggggtaaca gaattatctg cagaagaacg tctattgcat
     1081 gcgatctttg gcgaaaaagc tcgtgaagtc cgcgatacat cattacgtgt accacatggc
     1141 ggcggcggta tcgttcacga tgtgaaaatc tttactcgtg aagctggaga tgaattgtca
     1201 ccaggcgtaa acatgttagt tcgtgtttat atcgttcaaa aacgtaaaat ccatgaaggg
     1261 gataaaatgg ccggtcgtca cggaaataaa ggggttgtat cccgtattat gccggaagaa
     1321 gatatgccat tcctacctga tggaacacca atcgatatca tgctgaatcc tttaggggtg
     1381 ccttcacgta tgaacatcgg gcaagtattg gaattacact tgggtatggc tgcacgtcag
     1441 ttaggtatcc acgtagcaac gccagtattt gatggtgcaa gtgatgaaga cgtatgggaa
     1501 acagttcgtg aagccggtat ggccagcgat gcgaaaactg ttctgtacga cggacgtaca
     1561 ggtgaaccat ttgatggacg tgtgtctgtc ggtgtgatgt acatgatcaa acttgctcac
     1621 atggtcgatg acaaattgca tgcacgttca atcggacctt actcattggt tacacaacaa
     1681 ccattgggag gtaaagctca atttggtgga caacgttttg gtgaaatgga agtatgggca
     1741 ctggaagctt acggtgcggc ttatacttta caagaaatct tgacatacaa atcagatgac
     1801 gtagtcggac gtgtgaaaac atacgaagca atcgtcaagg gtgaaccaat tccaaaacca
     1861 ggtgtaccgg aatcattccg cgtattggtg aaagaattgc aatcactagg tctggacatg
     1921 cgtgtcttgg atatcgaaga aacagagatc gaacttcgcg acatggacga tgaagacgac
     1981 gatttgatca ctgttgatgc tttgacaaaa tttgccgaac agcaaactgc caaagaatta
     2041 gaaaagaaag ctgctgaaca agtggaagat gaaaaagacg atgtcatcca aaactttgaa
     2101 accgcagaag ataatttaga ctaatccggt ctatgagctg caaggcggtc gaagatcagc
     2161 cgtcttgcgg tttgccatta aatgaaatgg agggaacccc ttttgatcga tgtaaataaa
     2221 ttcgaaagta tgcaaatagg tttggcttct cccgaaaaaa tcagaagctg gtcttacggt
     2281 gaagtaaaaa aacctgagac gattaactat cgtaccttaa aacctgaacg cgaagggttg
     2341 ttctgtgagc ggatttttgg tcctacaaaa gactgggaat gtgcctgcgg aaaatacaaa
     2401 cgtatccgtt ataaaggaat cgtttgtgat cgatgcggag tggaagttac tcgttcgaaa
     2461 gttcgccgtg aacgcatggg acatattgaa ttagcagctc ctgtttcaca tatctggtac
     2521 tttaaaggga ttccttcacg tatgggtctt gtattagaca tgagcccacg cgctttggaa
     2581 gaaatcattt attttgcttc atatgtagtc atcgaaccag gaaatacgaa tttagagaaa
     2641 aaacaattgt tgacagaacg cgaatatcgc gaaaaacgtg aacaatatgg acaagaattc
     2701 caagctgcta tgggtgcaga agcgattaaa caattacttg ataatgttga tctagatgca
     2761 gaagcagccg aattgaaaga agaattgaaa tctgcacaag gacaaaaacg tacacgtgca
     2821 atccgccgtt tggatatttt agaagcattc cgcgcttcag gaaatcaacc aagctggatg
     2881 gttatggacg ttatccctgt tattccacca gatttacgcc caatggttca attagaaggt
     2941 ggacgttttg caacaagtga cttgaatgat ctttaccgtc gtgtaatcaa ccgtaataac
     3001 cgtttgaaac gtttgttaga cttgaatgca cctggaatca tcgttcaaaa tgaaaaacgt
     3061 atgctgcaag aagcagtaga tgcattgatc gataacggtc gtcgcggacg tccagttact
     3121 ggaccaggta accgcccatt gaaatctctt tctcatatgt tgaaagggaa acaaggtcgt
     3181 ttccgtcaaa acttgctagg taaacgtgtc gactactcag gtcgttccgt tatcgttgtt
     3241 ggacctttct tgaagatgta ccaatgtgga cttccaaaag aaatggccat tgaattattc
     3301 aaaccattcg tgatgcgtga gcttgttcaa cgcgaactag cttcaaacat caaaaatgcg
     3361 aaacgtaaaa tcgaacgtca agaagacgaa gtatgggatg ttttagaaga cgtaatcaag
     3421 gaacacccag tattgctgaa ccgggcacct acgcttcacc gacttggtat ccaagcattc
     3481 gaaccagttt tagtacaagg acgcgcaatc cgtttgcatc cacttgtatg tgaagcttat
     3541 aatgccgatt tcgatggaga ccaaatggct gtccacgttc cattgaatga agaagcacaa
     3601 gcagaagcac gtatgttgat gttagctgct caaaacatct tgaaccctaa agatgg
//
LOCUS       DANKHJ010000186         3615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000186 DANKHJ010000000
VERSION     DANKHJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(217..660)
                     /locus_tag="PW113_003233"
     CDS             complement(217..660)
                     /locus_tag="PW113_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2527466.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(794..1132)
                     /locus_tag="PW113_003234"
     CDS             complement(794..1132)
                     /locus_tag="PW113_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2527467.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(1120..1497)
                     /locus_tag="PW113_003235"
     CDS             complement(1120..1497)
                     /locus_tag="PW113_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2527468.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            1721..2914
                     /locus_tag="PW113_003236"
     CDS             1721..2914
                     /locus_tag="PW113_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2527469.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(3080..3502)
                     /locus_tag="PW113_003237"
     CDS             complement(3080..3502)
                     /locus_tag="PW113_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527470.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          964 a          670 c          658 g         1323 t
ORIGIN      
        1 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
       61 gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt tatgtaaatc
      121 tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc atacgtctcg
      181 cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag cttctttgaa
      241 acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc agactagcat
      301 gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg aaagatgact
      361 tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg caaatacatt
      421 gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc ctaaccagtc
      481 agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc
      541 ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga gcaatcccca
      601 tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg gatctagcaa
      661 aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac ttgttccgct
      721 gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta atagtgccaa
      781 tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga ataattgttt
      841 ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt cttctgctcg
      901 gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg cgtacaaaca
      961 gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa
     1021 gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc caaaaatatt
     1081 ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac attccatttt
     1141 atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt ccaaacactc
     1201 ttgcatttca gcaatttctt tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg
     1261 ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca aagactcgat
     1321 cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt cttcttctgt
     1381 ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga ttcgttcata
     1441 atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga tattcatctg
     1501 atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat tctacaactt
     1561 taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta tctatatcgg
     1621 ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt tggatatact
     1681 tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa
     1741 ggtattatta tcgacatcgg cgggaattgc tttagagaat atggatatta tgtttttagc
     1801 tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag cgggtttcat
     1861 ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca tcatagctga
     1921 taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga ttgcttcttt
     1981 attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta tcgcaggtgt
     2041 aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa
     2101 acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg cggctttggc
     2161 tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt atgtcgtagg
     2221 tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt
     2281 caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg aatcaaaaca
     2341 tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg ccggctattt
     2401 tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt cggtttctgg
     2461 gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga tgacttttgg
     2521 cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa tttgttcatc
     2581 cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag cagccgctgt
     2641 gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga ttagtagttt
     2701 gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg gacgagctgt
     2761 tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt taactgccgt
     2821 aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa ctcttactgg
     2881 tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa
     2941 gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa aacatgagac
     3001 actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca actgctaatt
     3061 tccttctatc tggcggcgac tattctttga cgtccgtcat ttcaaagtac tttactaata
     3121 acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca taatcttcca
     3181 aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat gattttctgt
     3241 agaaatgaag ataaataatg tagattggga agttctcctg cttttccata gaaaagcgcc
     3301 gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat tcttctaaat
     3361 ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt agttcttcta
     3421 ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc ttttgagaaa
     3481 cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta tactacatac
     3541 aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca taaacctgtt
     3601 attatttctc ttttt
//
LOCUS       DANKHJ010000187         3535 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000187 DANKHJ010000000
VERSION     DANKHJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            284..1324
                     /locus_tag="PW113_003238"
     CDS             284..1324
                     /locus_tag="PW113_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2527471.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2148..2480
                     /locus_tag="PW113_003239"
     CDS             2148..2480
                     /locus_tag="PW113_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2527472.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3099..3440
                     /locus_tag="PW113_003240"
     CDS             3099..3440
                     /locus_tag="PW113_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2527473.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1306 a          470 c          658 g         1101 t
ORIGIN      
        1 tttatttttt gttttgacac tggtagtact taattatctt taattttaaa actaaatttt
       61 attcatacgt ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta
      121 atgactttaa aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa
      181 aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg
      241 taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt
      301 ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt
      361 ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc
      421 aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac
      481 ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat
      541 caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca
      601 gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga
      661 tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg
      721 tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga
      781 gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc
      841 agaacttaat aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg
      901 cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc
      961 tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga
     1021 agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtga
     1081 tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata
     1141 cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa
     1201 cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc
     1261 aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact attacgaaag actggttaga
     1321 gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt
     1381 tttattattc taattatatt acgaatatta caaaataata gctattttaa tagaatttat
     1441 aagttaaaat attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag
     1501 aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag
     1561 ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat
     1621 tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt
     1681 ctgctatgct tgtgatgaat tgattttcgt tttgcacttt tggatgataa atttggacaa
     1741 ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc
     1801 gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt
     1861 acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt
     1921 taaggttaaa aatacgtgat attacgatga atctatgcct aagcataatg ttataaaaat
     1981 gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat
     2041 gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac
     2101 aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag
     2161 gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa
     2221 tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa
     2281 tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa
     2341 ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc
     2401 aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga
     2461 tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct
     2521 tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt
     2581 agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta
     2641 acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg
     2701 tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag
     2761 cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa
     2821 taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag
     2881 tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct
     2941 actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt
     3001 aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt gatataaaac
     3061 atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata
     3121 tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg
     3181 tgacgtttag aaggggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg
     3241 atttatacga tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg
     3301 atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg
     3361 ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg
     3421 tagcggataa agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg
     3481 agtcacgacg attaataaaa aggtagtatt accttaggca ttgagtaagg aattt
//
LOCUS       DANKHJ010000188         3513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000188 DANKHJ010000000
VERSION     DANKHJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..554)
                     /locus_tag="PW113_003241"
     CDS             complement(<1..554)
                     /locus_tag="PW113_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2527474.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIG"
     gene            complement(880..1770)
                     /locus_tag="PW113_003242"
     CDS             complement(880..1770)
                     /locus_tag="PW113_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2527475.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1967..2449)
                     /locus_tag="PW113_003243"
     CDS             complement(1967..2449)
                     /locus_tag="PW113_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527476.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2657..3022
                     /locus_tag="PW113_003244"
     CDS             2657..3022
                     /locus_tag="PW113_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527477.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            complement(3113..3430)
                     /locus_tag="PW113_003245"
     CDS             complement(3113..3430)
                     /locus_tag="PW113_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2527478.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
BASE COUNT         1051 a          675 c          546 g         1241 t
ORIGIN      
        1 cctatttctt tggttatttc atctgcttcg cctgtcaata aagcctcatt aacttgcagt
       61 ttcccttcca cgacctctgc atctgctgga atttgatgtc cagtctctaa aataacaatg
      121 tcatctatta caaggtcttc tatatctatt gtttgttcta tcccgtctcg tatgacattg
      181 acttttgctg tatttaaaac atttagttcg tccaagatct ttttggatcg aatttcctga
      241 aaaatggcga ttcctgtatt ggctatgata actggtaaaa aagtcaagtt cttataggat
      301 tctactaaac aaagcaaaat ggctaagata agaaagatca aattgaaata cgtaaatatg
      361 ttttccaata cgatttgctg atttgttttg aatgtgggat cgactgaatt atttgctgca
      421 cctttttcta ttcgttcctt gacttgatct gccgttaatc cttctttata cgatggaaaa
      481 agacggatga ctttatctga tgatctttgt gttactttct tttttttact tcgatttatc
      541 catttattct tcatgtggct acctcgattg atttcgtttt ctggatattt ccaaacttct
      601 cctatccagt atagaacaga tatggaaagt gttcctcaca ttaagcttgc aaacaccttt
      661 cattcctgtc atatagcaaa aaaaaagcta cgacagttct atgtcgtagc tctttttttc
      721 gaataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
      781 ggaactattt ttcacaatta aaggaaaaat gtatatgtgg ggaaacatag ttgatttttc
      841 cttaatagta acgactaccg tggcatgttt atctgctagt tatttgatat ttccttcgtg
      901 atcacgttct acttccatca ttgtgattgg taaatagccc aattctttga caggtccttc
      961 ttggatttct tctgtcatca tgtagtctaa gaatttttgg acttcagggg aaggttttcc
     1021 gttcgtgtac atatgttcat atgaccagat tccccagctg ttatctgcta cattctcttc
     1081 ttttggttct acaccgtcga tacttagtgc ttgtgtggaa tcatcgagat atgagaaagc
     1141 taaatagctg attgctcctg gtgtttgact taccagctgc cgaactgttc ctgatgaatc
     1201 ttgttcttgg gactgcacag gggtagcacc atccagtccc catttttcga atgtcgcgcg
     1261 ggtaccgctg ccgtttgcac ggttcacgac gttgattttt tgatctttcc cgccaacttc
     1321 cttccagtta gtgatttttc ctgtaaagat atcaatcaat tcctgtttgg tgatatcttt
     1381 gacatctgta tctttattga cgattggtgc catcccgacc actgctactt tatgatccac
     1441 taattttgaa gcatcaactc cgtctctttc ttctgcaaat acatcagagt ttccgatagt
     1501 aactgctcca gcttcgactt ggctcaaacc agttccgctt cctcctcctt gtacagagat
     1561 ttgataattt ggattttctt gtacaaagct ttcttgtgct gcatcgacta acggctgtaa
     1621 tgctgtcgaa cctaccgcaa cgatttttac ttgttcattt gagtcacttg aattattttg
     1681 tgtggcagag ccatctgtat ttcctgatcc tgagccgcat ccggctaaaa gaattcccat
     1741 catacctaga gtaaacaata cttttttcat tttattgctc ctttcttatt tacaactaaa
     1801 gtttatcagt cgactgtaaa tccctcgtat ggttgctgta aaaatatagt taagttatgt
     1861 aaaaatcatg taaacgtttt tcgcattctt tacataataa taaaaaaagc atatgagaaa
     1921 catgcatttt acgcagctca tatgcttttt tactgatgat ccaccatcaa tagttatttt
     1981 ttatagatac agatcgttca ctttttatcc tgatttctgt ccagaagtaa aaaaatgttg
     2041 caagaataaa tgaggcaatt actaaataga accatggttt gatcgtcaag aaatacatgg
     2101 aggaataagc tgaatccttc aatccactct cgctcaaacc attagggccg ataatcaagc
     2161 gaggcagcaa agaaaacata gatgtaaata ctaataagca agaaaagtaa agcagctgcc
     2221 aattgtagtg attgatcaga gattttggta ataagagacc tacacttatc actaaataga
     2281 aaacagtcaa agctggaaaa ttcctagaaa ccactaatcc gcttccttgg atcaggccta
     2341 atatctccgc accttgtaca acagcattta cccatgtaaa aggtagcaaa acgataggaa
     2401 aaaaccaaaa aaataaataa agccgatcga ttactgcttt tcttctcatt aacgttcccc
     2461 caaactactt tttctctcat catactatac aattttctat ctaaaaaaat tagggggaaa
     2521 aacttttttt ttagagataa tggcatgaga gtttattcat ttaatctaga aataatgaaa
     2581 aagtaattga ttattgcagg attcaattta caataaaact aacaaaataa taacacttgt
     2641 taggtggtga tgaaagatga taaaaacttg ctggaaaaac cttccgttgc ttttatcttt
     2701 tgttccttat gtgcatttcg ccttactgct tgattttcgt tatcattccg taagcgggtt
     2761 catcacattg atatttttat ctctttttgc tggatattat tttcaaagga acaggcgtat
     2821 tatatcttta ttcattgcca atattatatc tactgtcact tcctatttat tttgcgccaa
     2881 ttttactgaa tggcgttact tctatcatcc tttaaaaccg acccagctga tcttgcttct
     2941 tgcaggtatt tacttagttc cacaaatcct tgggtcgtta tgggcggtag ccttatctta
     3001 taaaaaagct cggcatccat aaaaacaaac catatatgta caaagagaag ctactttctt
     3061 ctttttatac atatatgttt ttcttatatc accaaaacta tcactagttt ttctattttt
     3121 tacttctttt cttctctcca aacactaaaa acagttgtgt aataattgca gcaataatga
     3181 ttccaataat agatattgaa tttgaggtgg aaacatctat aatattcaaa gaataaagtg
     3241 ttgaagaaag taaactaatt aaaacaattc ctccataaaa aatagtttgt gagtataccc
     3301 aatttttttt agaagacaga cttcgtttag ttctaaaacc tatacttgca tttctactcc
     3361 tatcaaaaat ccatatattc agaagtaata atatcgctac tattaagata aaattactat
     3421 aaaaaagcaa aatattacct cctaattaaa tattaaatac cgaaaaaatt atgtttagat
     3481 aataccatag ttaagttttt ccttgtgatg ttt
//
LOCUS       DANKHJ010000189         3509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000189 DANKHJ010000000
VERSION     DANKHJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(55..1557)
                     /locus_tag="PW113_003246"
     CDS             complement(55..1557)
                     /locus_tag="PW113_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2527479.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1816..>3509
                     /gene="uvrB"
                     /locus_tag="PW113_003247"
     CDS             1816..>3509
                     /gene="uvrB"
                     /locus_tag="PW113_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2527480.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDS"
BASE COUNT         1080 a          661 c          725 g         1043 t
ORIGIN      
        1 gtgttatcgc cgtatttttt gactaaatgc tcaactaaga ttttttctgc catcttactt
       61 caccttcttt tctaaaactt ttgctaattt tgtcaatgct gtgatcaaaa tcaagtacat
      121 gatcgcaatg atcaagtaaa catacgtact ttgtgttgta cgtgcaacaa tgatcttacc
      181 agtttgcagc aattcgacta caccgattgc ggaaatgatc gttgtgtctt ttaatgagat
      241 aacgaactga ttgacgaatg atgggatcat gatgcgtatc gcttgcggca aaatgatttt
      301 ttgcattgtc cgattataag aaagacctaa acttctggaa gcttccattt gtccgacagg
      361 aacggcgttg atcccgcctc gaacaatctc tgcgatataa gcacttgcgt taagagtcaa
      421 ggtgataaca ccagcaagga agtcaggaat cgtaaatcca acgataccag gcaacccaaa
      481 gaaaataaag aacgcaagaa ccatcattgg aattcctcgg atgatatcga cataaatact
      541 agcaattgtg cgaagagcgc gtattggtgc tacactgaat aatccaaaaa tcacaccgat
      601 tactatagcc aaagcaaagg agatcaaagt taggagaatt gttctccaaa gtcctttcaa
      661 caatgcttga tagttgtttt ggataagtcc aagaaatgtt gattcatcag cagtggaaga
      721 ctcactggaa gtttctgaac catccgctac atattcttta ataatcttat cgtattcgcc
      781 tgttcttttt aattctttca atccttcatt gaacatatta agaagctcag gattttgtcc
      841 ttttttaacg gcaaatgcat attttccgcc agattctttt ttgatcggtg tttccaattc
      901 ttgtccttgt ttgatcgcat aaccgattac cggataatca tccatcattg catcgattgc
      961 gccaactttt aacccgtcgt acaattgatc agcagcatca aaatatttga tagaatagcc
     1021 atattcagat tcatggtcgc ttaaaaattc agcactttcg gtaccaactt ttgctccgac
     1081 tgtttttcct ttcaaatctt cataagactt gatcgaagta tctccttttt tgacagctaa
     1141 ttggatcccg ctatcaaagt aagaatcaga aaaatcatag cttttctttc gttcatctgt
     1201 tacagtcatt ccggcaatca ttccgtctgc ttgtcctgat tccaaagctt gcaaagcagc
     1261 agagaaacca cggtagtcca ttcggatgtt aaatccttgc agtttggcga tgttttccat
     1321 caattttaca tcgatgcctt cgtactcgcc ctttgcgttt tgaaattcaa atggagcaaa
     1381 agcagagtca ctggcaatta cgtatacgtc ttttttgggc tctatttttt gcattgtctc
     1441 gcttgtgctt gactctgccg ttgtagtgtt gtcagcttgt gcaacatgtc caaacgcaag
     1501 tgtcacaagt cctgtcatta agaaaacaaa tgagtgaaat aaatgctttc ttttcataat
     1561 caacctccta aatttccaat attttattat tataggcgaa aagtgctgaa tcagacaaga
     1621 ctgtaagctc gaaaaaatga aaaaaatgcg agtttctaac attttatgtt ataaaacgta
     1681 acatgcataa taagataaaa aagtggcgaa agtatgttcg gtgtggtata attttttagt
     1741 aaggaatgcc tttgctttcc ttttcttttt attggtcgta aaatgaaaag gaatgaatga
     1801 aaaggaggac gaagaatgat tgagagagat acctcccgac actttgagct tgtctcgaaa
     1861 taccaaccgg ctggtgatca gcctgaagcg attaaccaat tagtggatgg cgtagtcggc
     1921 gggaaaaaag cacaaatctt attaggtgcg actggaacag ggaaaacata tacgatttcc
     1981 aatcttattg agaaagtgaa taaaccaaca ctaattattg cccataacaa aacattggct
     2041 ggtcagctgt acggggaatt caaggaattt ttccctaaca atgctgttga atattttgtt
     2101 tcttattatg attattatca gcctgaagct tatgtgcctt caagcgatac gtatatcgaa
     2161 aaagattcaa gtgtaaatga tgagatcgac aaattacgac attcggctac tagttcatta
     2221 ttagaaagaa atgatgtgat cgtcattgca tccgtttcat gtatttttgg attaggttcg
     2281 ccttttgagt accaaaaaca agtagtttcg attcgacagg gagcagaact agatcgcaat
     2341 cagttgattc gtgacttagt cagtatccaa ttcgaaagaa atgatataga ttttcagcgc
     2401 ggtcgttttc gagtgcgcgg tgatgtcgtg gagatttttc cggcttcaag agatgaacgt
     2461 gccttacgtg tggagttttt tggagatgag gtcgaacgta tccgagaagt caatgcactg
     2521 acaggtgaag ttttaggaga aacggaacac gtggcaatct tcccggcgac tcactttgtg
     2581 acgaatgatg aacatatgga gcatgccgta gcgaatatca aagcggaatt agaacagcgt
     2641 ttaactgtct tgcgaaatga aaacaaactt ctggaagctc aacgtttaga acagcgaacc
     2701 aattacgata ttgaaatgat gttagaaatg ggctacacgt cgggaattga aaattattct
     2761 cggcatatgg acggacgtaa agaaggtgaa ccgccttata cgctattgga tttctttcct
     2821 gaagattttt tgatcgtggc ggacgaatcg cacgtaacca tgcctcaagt cagaggaatg
     2881 tataatggtg accgagcgag aaaacagatg ctagtagatt acggtttccg tttgccatct
     2941 gctttagaca atcggccact tcggttagaa gagtttgaaa aacatgtcaa tcagatcatt
     3001 tatgtctctg cgacgccagg accatatgaa catgaacaaa cagataccgt tatccaacaa
     3061 atcatccgtc cgacagggct gcttgaccca gtgatcgaag ttcgtccaat catgggacaa
     3121 atcgatgatc tagtaggcga aataaatgaa cgagtcgaaa aagaccagcg ggtgtttgta
     3181 acgacattga cgaaaaaaat ggcagaagac ctcacagatt atttcaaaga attagggatc
     3241 aaagtcaaat atttgcatag tgatatcaaa acgctggaac gaacagaaat cattcgagat
     3301 cttcgattag gtgaatttga tgtgttagtt ggaatcaatt tgcttcgtga aggattagac
     3361 gtacctgaag tttcccttgt tgctatcttg gatgcggata aagaaggctt tttacgtagt
     3421 gaacgttcct tagtccaaac gattggacga gctgctcgga atgaagaagg taaagtaatc
     3481 atgtacgcag acaaagtaac agattctat
//
LOCUS       DANKHJ010000190         3424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000190 DANKHJ010000000
VERSION     DANKHJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            24..665
                     /locus_tag="PW113_003248"
     CDS             24..665
                     /locus_tag="PW113_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2527481.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            684..1319
                     /locus_tag="PW113_003249"
     CDS             684..1319
                     /locus_tag="PW113_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2527482.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            1337..2152
                     /locus_tag="PW113_003250"
     CDS             1337..2152
                     /locus_tag="PW113_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2527483.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            2471..>3424
                     /locus_tag="PW113_003251"
     CDS             2471..>3424
                     /locus_tag="PW113_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2527484.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGK"
BASE COUNT         1133 a          573 c          744 g          974 t
ORIGIN      
        1 ttattttaaa ggaggaaaag aagatggagt atttgattga agttatgccg caattattag
       61 taggagcact cacaacattg aaattatttt tatttacctt gattggctca ttaccattag
      121 ggattgtttt agcattagga ttgtctttgc attttaagcc gctacgctac ttgcttcaaa
      181 catatgtatg ggttatgcgg ggaacaccgc tgttgttgca actgattttt attttttatg
      241 ggttgccgac gattgggatc gtttttgatc gtttcgaagc agcactcatt gcctttatct
      301 taaactatgc agcttatttt gcagagatct ttcgtggagg gatacaatcg attcctcaag
      361 gtcaatatga agcagcgcaa gttttgcaat taagtccctt tcaaacggtg acgaaaatcg
      421 tcatcccaca agtgacaaag attgttttac cttctgtagg gaatgaagtt atcaacttgg
      481 tcaaagattc ttccttgatc tatatattag gtttaggtga cgtaatgcgt gctgggaaaa
      541 tcgcgatgga acgcgacgcg actttactgc ctttgctagg cgtggcaatc atctatctta
      601 cattgactgg tattctaaca atcgtaatga aacaagttga aggaaaatta aattattacc
      661 gctaactgat aggaggaaga aatatgattg aattgaagaa agtgaacaaa gcatttggtg
      721 aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga tgggaaaaca ttagctatcg
      781 taggtccttc cggtggcggt aaaaccacgc ttttacgtat cttggctggc ttagaaagtt
      841 ctgacagcgg agaattttat ttagacggtg taccgttcaa cccattcgag cttaaaatga
      901 aagaacaagt tgtaggagtc gtattccaag acttccagct atttccacat ctgaatattt
      961 ttgaaaacat taccttagca ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca
     1021 aaaaagcaga agaattagcg gcttcattag ggattagtga tttattgaat atctatcctt
     1081 atcagctttc tggcggacaa aaacagcggg tagcgattgc cagagcattg gctatgaatc
     1141 cacgggtatt ggcttatgat gaaccaacaa gtgctttaga tcctgaatta cgccagcaag
     1201 tcgagcgttt gatcctatca ttgaaagaag atggggtaac ccaaattatt gtcacccatg
     1261 atttagtatt tgcagaaaat gtcggtgatc aattactaaa agtttcccca attcattaag
     1321 aaaggagaag atagagatga aaaaaaccag ttttctattc gctttgatcg caggtcttct
     1381 tctttttgca ggctgttcga acaaaaaaac ttcagcagat aattgggcga ctatcaacga
     1441 agagaaaaaa atcgttatag gtttagacga tacatttgtt cctatgggat ttagagataa
     1501 agacgggaag ctcacaggat ttgatattga tttagccaaa gcagtctttg aacaatatgg
     1561 gattacggta gactttcagc ccattgactg gtcaatgaag gaatttgaac tgaacaatgg
     1621 aacgatcgat ttgatatgga acggctattc caaaacgccg gcaagagaaa agaaagtgca
     1681 atttacaaaa ccttatatgg aaaacgatca agtgctgatc acacctaaag aatcaggaat
     1741 cacaaacttt ggacaaatga ataataagag attaggtgcg caaaatgggt catctggtta
     1801 tgatgtattt acgaaacaac cagaagtatt aaaagagata gtcaaaaacc aagatgctgt
     1861 tttgtatgat agttttaatg aagcattgat cgatctgaaa agcagacggg tcgatggtct
     1921 gctgatggat aaagtatacg cagactatta tttgaaacaa agaaacgaac tagaagactt
     1981 caacataaca aaaggttctt atgagagtga ggattttgca gttggtgcac gcaagtcaga
     2041 tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt
     2101 ccaaaaaata tccgataaat ggtttggcga agatgttacg ccgagaaatt aacaaatagg
     2161 ggactgtgac aaaagacgtg tcacagtccc tttttccgaa taaacagtgt tctcctgcca
     2221 actccttgtg actcggagct gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa
     2281 agccagtttt tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
     2341 atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcctttaca taattttatt
     2401 ttgttgtgga aggggtttcg tgttattcta aaagaggaac gattgaaggt tataggaaag
     2461 gaagagatga atggaacaca aagaacttac ctcatttcca aaagattttc tgtggggctc
     2521 tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag gagaatcagt
     2581 ctgggatcga tttgtacgga tacctggaaa gacatttaaa ggaacgaacg gtgatttggc
     2641 agtagaccac tatcatcgtt ataaagaaga tattgcttta atgaaagaac aaggtctgaa
     2701 agcttaccgc ttctctgtag cttggacacg gatttttcct aatggacgag gagaagtcaa
     2761 tcaagctggg ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa acgatattga
     2821 acctatgttg actttatatc attgggatct tccacaagca ttgcaagatg aatataatgg
     2881 ttgggaatca cgtcagatca ttgaagattt cactcattat gcagaaacat tatttgaggc
     2941 attccgcggg aaagtccatt actggattag tttgaatgaa caaaatatct tcacttcatt
     3001 aggttatttg ttagcagcac atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt
     3061 taaccatatt gctaatttag caaatgcatc tgtcatcaat aaatttcacg agttagaaat
     3121 gcctggaaaa atcggaccaa gctttgcgta tacgccaaat tatccaattg acagtaatcc
     3181 tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg gccaattatt ggatggatgt
     3241 ctatatgtgg ggaaaatatc cgattgctgc catgaggttc ttagaagaaa aaggatgggc
     3301 accaacgatt gaagctggcg atgcagaatt gctagaatct gctaaaccag attttcttgg
     3361 gatcaattac tatcaaacgg cgacgaatgc attcaatcca ttaaacggag ttggtgctgg
     3421 aaaa
//
LOCUS       DANKHJ010000191         3389 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000191 DANKHJ010000000
VERSION     DANKHJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..141
                     /locus_tag="PW113_003252"
     CDS             <1..141
                     /locus_tag="PW113_003252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527485.1"
                     /translation="KDGPFLSKEDLATRGKVSKTLIEYMNDNGVLKDLPDENQLSLFD
                     ML"
     gene            285..758
                     /gene="rimP"
                     /locus_tag="PW113_003253"
     CDS             285..758
                     /gene="rimP"
                     /locus_tag="PW113_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2527486.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            782..1957
                     /gene="nusA"
                     /locus_tag="PW113_003254"
     CDS             782..1957
                     /gene="nusA"
                     /locus_tag="PW113_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2527487.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            1979..2272
                     /locus_tag="PW113_003255"
     CDS             1979..2272
                     /locus_tag="PW113_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2527488.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            2269..2580
                     /locus_tag="PW113_003256"
     CDS             2269..2580
                     /locus_tag="PW113_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2527489.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            2593..>3389
                     /locus_tag="PW113_003257"
     CDS             2593..>3389
                     /locus_tag="PW113_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HDL2527490.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPD"
BASE COUNT         1290 a          608 c          710 g          781 t
ORIGIN      
        1 aaagacggtc catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca
       61 ttgatcgaat atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta
      121 tcgttattcg atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt
      181 atggtatagt tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc
      241 tttttaatgg aattacgcac ttaattttta aggaggcgaa acttttgagt agcgtcgttg
      301 aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag
      361 tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg
      421 gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag
      481 cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc
      541 ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct
      601 tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg
      661 aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa
      721 aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa
      781 aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa
      841 agaaatcgtg atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg
      901 ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc
      961 ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc
     1021 acttttaatc aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa
     1081 agattttgga cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga
     1141 agcagaaaga acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg
     1201 aatcgtcgaa cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt
     1261 tctttctaaa caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt
     1321 ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca
     1381 tccagaccta ttgcgtcgct tatttgaaca agaagtacca gaagtctatg atggaatcgt
     1441 tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa
     1501 tgatccaaat atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc
     1561 aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc
     1621 tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac
     1681 aaatccaaag gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa
     1741 acgcggacaa aatgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc
     1801 agaatctgac atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact
     1861 gcatgatgaa gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt
     1921 caacaatgaa acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat
     1981 gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa
     2041 agagatggtc cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa
     2101 aatgcctgga cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga
     2161 taaaaaaatt ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact
     2221 tcttgactat gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt
     2281 aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg
     2341 ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca
     2401 accgatgcaa gtgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt
     2461 ccttgctttg agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag
     2521 gtattcggaa taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag
     2581 gaaggtgatt gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct
     2641 agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt
     2701 gcgattacta gcagtgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa
     2761 caacaagctg cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt
     2821 aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc
     2881 ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg
     2941 ggaaatacca gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact
     3001 aacaataaca acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat
     3061 cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca
     3121 ccacgcaaat tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca
     3181 gatattgcga aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt
     3241 ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg
     3301 gactatggca tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc
     3361 ttcgaggcag aagaagtcga tccagataa
//
LOCUS       DANKHJ010000192         3388 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000192 DANKHJ010000000
VERSION     DANKHJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(63..2555)
                     /locus_tag="PW113_003258"
     CDS             complement(63..2555)
                     /locus_tag="PW113_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2527491.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="PW113_003259"
     CDS             complement(2562..3029)
                     /locus_tag="PW113_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2527492.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="PW113_003260"
     tRNA            complement(3165..3238)
                     /locus_tag="PW113_003260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
     gene            complement(3296..3369)
                     /locus_tag="PW113_003261"
     tRNA            complement(3296..3369)
                     /locus_tag="PW113_003261"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Pro,seq:tgg)
BASE COUNT          924 a          750 c          557 g         1157 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
     3301 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     3361 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DANKHJ010000193         3382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000193 DANKHJ010000000
VERSION     DANKHJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            80..880
                     /locus_tag="PW113_003262"
     CDS             80..880
                     /locus_tag="PW113_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDL2527493.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            896..1774
                     /locus_tag="PW113_003263"
     CDS             896..1774
                     /locus_tag="PW113_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2527494.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1787..2767
                     /locus_tag="PW113_003264"
     CDS             1787..2767
                     /locus_tag="PW113_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2527495.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
BASE COUNT         1172 a          553 c          705 g          952 t
ORIGIN      
        1 actaactctt gacagataat caaaaagaat ttatagttgt taccgtagta gctactaaaa
       61 ataaagtgag gattaatcaa tggaaacgat tattcaaatc atgaaaaaat atgatttttc
      121 agagatggag acaagagtgt ataccacact attagaaaaa ggtaatctta caggatatga
      181 agtaagtaaa atctcaggtg tgccacgctc gaaagtatat aatattttgg aaaaactatt
      241 aaaaaagaat ttgattgtcg ttaataaatc agaacctaaa ctttatcatg ccatctcggc
      301 aaatgagttt ttagagaaat tagaaaaaag tgttaaaaat gatctttctt ttcttaccaa
      361 gaatctaggg atgatcaaag aaaaagatga agaagaaatg ctgtggaaag ttgatggtat
      421 cgagtatgtt ctggataaag tagagcattt ggtgaaaaac gcaaaagaaa gtttgctcat
      481 tcaagtttgg catgagaatt tcacggattc actactaaag gcattgcagc aagctgaaaa
      541 aagagttgat aagtttgtac tgattttatt cagttctacc catgaatatg atcttccctt
      601 agaaaagtat tatattcatg gttttgaaac agacaaactt gctgactttg gagcaagatg
      661 gatcaacatc gtcgccgatg agcaagaagt cgtgtttggt accattaacg aagaactgca
      721 atcaaccgat gtcacttgga caaaaaacca cgccatggtc aatctagcaa aagaatatgt
      781 caaacatgat gcatatacac taaaagtgat tgcagaatct cctgaagaac ttaaagcaaa
      841 atatggtgag ggctttgagg gaatcagaaa gatttactag taggaggaat tttttatgat
      901 tcggaatatt ttgctgacac taattgtcgc aatcaatcta tattttatta tttactttct
      961 aagagacctt ttcaaaaaca aacaaaattt caaagaagaa ccaggggata tgcgtctgtt
     1021 gcccttcacg tcatttatca tcttctttct ttcaacattt ggcgtttccg actttgctat
     1081 cggaaccgtc ctttatccaa agttgaaatg ggtcagcatg aaaaaactac ccggaacgtt
     1141 gaatacccaa tgtgtagtac cggtagccgt catggcattg tcttacatca ctgccatcaa
     1201 cgtagggatc aagacactag ctgtttgtat tatctgccaa attatcggtg catatttagg
     1261 accaagattt gtcgtgaagt taccagagaa aacgatcaaa ctgtttgtcg gcataggatt
     1321 ggtcattgct gcattgttga tttttgcagg acaaatgaac tggattccat caaacggaac
     1381 agccagtgaa ttgtatggcg gaaaattaat cttggcagga tttttactct ttgtctatgg
     1441 tgcattgaac aatatcggca ttggttctta tgcgttaacg atggtaactg tctacttgct
     1501 aggcttgaat ccagttgcag cctttcctat tatgatgggg gcttgtacct tctcagtacc
     1561 gatcggcagt gttcaattca ttaaatttaa tgaatacagc agaaaaatca cactttttac
     1621 ttcaaccttc ggtgtattgg gggtattggt cgctgtattc ctagtcaaat ctttagatac
     1681 ctatatcctg aaatggatcg tgattttggt cttactatac agtgcatata cgatgttggg
     1741 cagtcaatta aaaaaagtta cagcagctaa ttaaggagcg attcaaatgt tatttcttaa
     1801 aaaagaagat attatcaaaa gtttttcaat gagagaagcc atcgatgcgg acaaaaaagc
     1861 actgtcattg tattcagcag gaaaggcaag tgtgcctctt cgcaccaata tcgatgtgcc
     1921 aaaaagcaac ggacaaagct tatatatgcc tgcttatgta gaaggtggcg aaggcgcgct
     1981 aggtgtcaaa atcgtttccg tgtatcctga aaacatcaag aaaaacttac caagtgtacc
     2041 agcaacgatg atcgtattgg atccagaaac gggtatggta tccgcttgtt tggatggtac
     2101 ttatttgaca caattaagaa caggcgctgt tcaaggagca gcaacagaac ttcttgcaag
     2161 agaagatgct aaaattggcg cgctgattgg aaccggcgga caagcacagt cacaattaga
     2221 agcaatgttg accgttcgta aattagaaga agttcggatt tttgatattg attttgagcg
     2281 tgccagccaa tttgctgaaa aaatgatgca acaattttct gtaacgatgc gcccaacaaa
     2341 aaccagtcaa gaatgcgttg aaggagcaga catcattaca agtgtgacca cttcaaaacg
     2401 tgcgacattc tctgctgagt gggtcaaaaa aggtgcgcac atcaatggag tcggtgctta
     2461 cacaccggaa atgtgtgaaa ttccacgaga aataatcaaa gccgcagatg ttgttatttt
     2521 tgataccatg gacggtgttc taaaagaagc tggggacttc atttcaccgc tgcaagatgg
     2581 gtatattcaa agagacagct atcatgggga actaggccaa ctgatcaatg aagaattgat
     2641 cggtcggaca agtggagaac aaatcacgat cttcaaaaca gttggttcgg ctgtattaga
     2701 tgtggtagtt gcgacagaaa tcgttaagaa agctaaagaa aataatttgg gaaaattgat
     2761 tttctaatta aaaaggctga aattaaaacc tttattattg cgtttaaaaa aagaaaacaa
     2821 agaagtttgt aatcaaatgg aaactgaccc cataatttaa ataaaataaa aaacatctct
     2881 tgttgaattc tagtaatgaa gcgaccctaa aacttagacc aaaaaatcta acttttgggt
     2941 ggagttgtca agaaaaaccg ggcacttagt taaaaaataa gttggacggg ttatactaaa
     3001 attgacacca aattaagaga aagcgaagat ctggattatg ccaagacgtt catttgatga
     3061 aatatttaaa attaccgctg taaaactggt aacggaagaa gggttttctg ttaaagaagt
     3121 tagtctatag ctagaagctc gctctaatag cgtgtattga tgagttcaag aagttgaaaa
     3181 atatggataa cgtacttttc tcggtagagg aagtcatgaa atccattcgc agaaatgaaa
     3241 ttacacgctt agaaaaagag aataaacggt taaaagaata ggtagaactc ctaaaaatta
     3301 ccgaagtctt cctgaaagaa aaggctcttc gtcaaattgt gttggcgaag acaaatttga
     3361 ataaaactta gtccgatatg cg
//
LOCUS       DANKHJ010000194         3352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000194 DANKHJ010000000
VERSION     DANKHJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1450)
                     /locus_tag="PW113_003265"
     CDS             complement(<1..1450)
                     /locus_tag="PW113_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2527496.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGE"
     gene            2178..3146
                     /locus_tag="PW113_003266"
     CDS             2178..3146
                     /locus_tag="PW113_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2527497.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            3164..>3352
                     /locus_tag="PW113_003267"
     CDS             3164..>3352
                     /locus_tag="PW113_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527498.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTA"
BASE COUNT         1021 a          685 c          646 g         1000 t
ORIGIN      
        1 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
       61 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
      121 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
      181 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
      241 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
      301 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
      361 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
      421 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
      481 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
      541 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
      601 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
      661 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
      721 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
      781 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
      841 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
      901 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
      961 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     1021 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     1081 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     1141 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     1201 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     1261 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     1321 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     1381 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     1441 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     1501 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     1561 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     1621 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     1681 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     1741 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     1801 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     1861 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     1921 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     1981 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     2041 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     2101 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     2161 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     2221 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     2281 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     2341 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     2401 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     2461 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     2521 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     2581 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     2641 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     2701 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     2761 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     2821 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     2881 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     2941 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     3001 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     3061 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     3121 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     3181 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     3241 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     3301 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag ct
//
LOCUS       DANKHJ010000195         3224 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000195 DANKHJ010000000
VERSION     DANKHJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(238..387)
                     /locus_tag="PW113_003268"
     CDS             complement(238..387)
                     /locus_tag="PW113_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527499.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            849..1061
                     /locus_tag="PW113_003269"
     CDS             849..1061
                     /locus_tag="PW113_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527500.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(1339..3138)
                     /locus_tag="PW113_003270"
     CDS             complement(1339..3138)
                     /locus_tag="PW113_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2527501.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         1145 a          561 c          565 g          953 t
ORIGIN      
        1 taaatattat acagataata acggaatatt atcatcgaaa cagctaacaa aattgtcata
       61 aaaaacgaga tggttgatat tagaacttgt gtttttgttt aggtctagag ataaagagag
      121 acaatttcca aatatatgta atgaatacaa aaagacgttt gtcgcggatt accgttacaa
      181 acgtctttta ttgattttcc gagatctcat cagtcgccat ttggaatggt tcactcctta
      241 tctagattcg tttaatgctc ggactgcttg ttcgattgta ataccaaacg caacgttatt
      301 ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt gtatcaaaag ctacgcggta
      361 ttgcatattt gtttttgtaa acgtcataaa attcatctcc tttgtaatgt aatttagttc
      421 ttgaaagttt cctttaaact ttcgaactta taccaagttt agcatcgtac gttttttgat
      481 gcaattaaaa agcatttatc attttcctta tttatttttt cttaaatggt ttagctattt
      541 tcttttaatc cgataagtag cccaatcatc aaggttacaa atactattgc ttgaatataa
      601 atcatatgct cacctcttta taaataactt agcaatataa agaaaaaaac gcacgaacaa
      661 cataggaata taaacaaacg tttttgtcac aacgtctaag atcgtgttaa aaaatagtta
      721 tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa atgaataaaa acgttttcaa
      781 atacaaggta atttgttatt ataaaataaa gaaaaaacaa aataaaaata aagaaaagag
      841 ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat atgcaaaccg atcggagtat
      901 ctcatgaact aatcggctgt attgaacgta catatatcaa cacggctgtc gtgtatgttg
      961 aaagttatga ccaacgagac tatcaactta tcaatgaaag caagtatcgc atgctcgtaa
     1021 aattctgtga tattagcgca cctactgaat tggtttctta aacagtattt ttgagaattc
     1081 gcaagaactc ccttataata taaataaaga cagaagggag cgagaagata tgccaaaaag
     1141 aaaaagttca gggaaaatgg gaattattct tgcagtgatc tctgcaattc ctgctatttt
     1201 agctattgtt cgtgcgatga aggaatcaag agaaagttaa gcacacatta tgagcgtcaa
     1261 acaaaaaacg catatacctg cggtggcagg ttatgcgttt tttgttgtat aaagactttt
     1321 ttcattaacg atttttgatt agccgactcc caaaacaaaa tttagcagtt tatctgaata
     1381 agtgatttct tcatctgttt gtttcacact ttcatttagt agtcccatct catcggttat
     1441 gataccatcc acaccataaa acatcatgcg actcatggca tctgaatcgt tgattgtcca
     1501 tacatatact tcttttccgt cttggtgggc agaattaatg aagttacggt tgatcgtact
     1561 atattccatt gtcagaaaat cagcaggcgt cacagggggc ccaacaatat tgaaaggtaa
     1621 gatgtagccc acataaaagt ttggttctgc ttcttttaaa tctgaaacgg tttggtacgt
     1681 caaactttgc aaaatatgat ggttctctaa gataactttg cggtatttat ggacaaatcg
     1741 ttcgacaagg tcatccgaat cttttttaga ggtcttgatc tcaataagta atttttggtt
     1801 tagcttatgt gctttttcca aataagaatc aaaagaacaa acaggggctt gcactccgtt
     1861 ttcagtaact gtaagatttg tcaattcttt caatgtaaga tcctgtggtg ttccatttac
     1921 ccctgttaag tgacgaagat ttgcatcatg catcactacg aaatgaccat cttttgtttc
     1981 ctgaacatcc atctctacat aatcaggatg atagtctctg cttgtttttt caagtgcttc
     2041 taaagtattt tgcgccccat tttgggtact cacacctctg tgagagacag tcactggttt
     2101 tgtctgaaca gctgaagtca gataatccat attgtaaaga cagacgccaa taccgaaaaa
     2161 aactgcaaaa agagtcaaac cagtgttttt cagcgcagag aaacgcaagt tgggggcttg
     2221 tgggataaac cacttaggga tctcaggcaa gaagccttcg tcatccatga aatcaacaat
     2281 gatataaaaa atccctacgg tagacatcac gatattgaat aataaaatcc cttgtaaaag
     2341 cgtcatagca aacacagcgc tgatcaatgc atagtctggg aaaaattgtt caaccatact
     2401 ttgtgccaaa ataacaacga tatatcctaa agaagaaagg agtaaaatcg taccgccaat
     2461 cacaataaat tggcccgtga tggccaaaag acgcgatttg gtcaatgacc agctttcccg
     2521 aatggctgct cgaaaagaac gatctcgtag aatcatttcc ggtaaagcaa aaatcagtcg
     2581 aatcccaata tagccaagga aaatataaac gagtaaaaag gaactgacga tgatccaacg
     2641 attagcaaaa ataaaatcca taatgaaagc ggggattttt atttttgaca gtagatcaga
     2701 attaaaactt aatccgctga tcggtagaat caataaaaaa taagcaagga aaaacaaaaa
     2761 agtaatgggt ctcactttct tcagctgaag tatcgtcaag tgtaagagtt gcttcaaaga
     2821 gattggttct ttctttttaa taaaaaaaac actcatcaaa agaaaagtaa attcgaaata
     2881 aactaacagt aaaatcgtca tcaaaactac gattaacgag agtagaaccc caggatgttg
     2941 agaaaaaatt gaaggaatat tgtcataaga caaatagtca attgcccctc ggtgtaaaat
     3001 aaaacgggtc gtactgctca ataagggtaa acaaataaat aaaataaagc catgcatcag
     3061 caagacatca cggaaataag cttgtgttcc ttttaaaaag tcccaagcat tacctaagct
     3121 acgtttcaaa taattcatcc tatcacctgc tcttcacatt gtagcgcaaa tctaatgaaa
     3181 aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga ttta
//
LOCUS       DANKHJ010000196         3208 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000196 DANKHJ010000000
VERSION     DANKHJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1863
                     /gene="ligA"
                     /locus_tag="PW113_003271"
     CDS             <1..1863
                     /gene="ligA"
                     /locus_tag="PW113_003271"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2527502.1"
                     /translation="ITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            1969..2274
                     /gene="gatC"
                     /locus_tag="PW113_003272"
     CDS             1969..2274
                     /gene="gatC"
                     /locus_tag="PW113_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2527503.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            2274..>3208
                     /gene="gatA"
                     /locus_tag="PW113_003273"
     CDS             2274..>3208
                     /gene="gatA"
                     /locus_tag="PW113_003273"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2527504.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIA"
BASE COUNT         1086 a          570 c          751 g          801 t
ORIGIN      
        1 atcacgtctg attctcccac tcaacgagta ggcggaaaag tattagaagg ttttgaaaaa
       61 gtcactcatg atattccgct ttacagtttg aacgacgttt tcagcaaaga agagctgata
      121 gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag ttgactattg ctgtgaacta
      181 aaaatagatg gactttccgt ttctttgcgt tatgaagatg gaaatttcgt gagaggtgcg
      241 actcgcgggg atggaactgt aggagaaaac atcactgaaa acctaaaaac tgtccggtcg
      301 gtcccaatca aattaaaaga accaatgaac atcgaagtaa gaggcgaatg tttcatgcca
      361 aaacggtcat ttgtccaatt gaaccaagac agagaagcag agggaaaaga tatctttgcg
      421 aatcctcgga acgccgcagc tggttctttg cgtcagttag attctaaaat cacagcgaaa
      481 agaaatctgg acactttttt atacacagta gctgatttcg gtcctatgca agcaaaaacc
      541 cagtatgatg cgctagaaga gctagaaaaa attggattcc acacgaatcg ggaaaaacgt
      601 ctatgtcatt cgattgatga agtgtggtca tacattgaag aatatcatga caaacgtgtg
      661 gatttgccct atgaaatcga tggaatcgtc atcaaagtga atgaattctc gctacaagat
      721 caacttggtt ttactgtcaa agcaccgaga tgggcagcag cttacaagtt tccgccagaa
      781 gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg gtcgaacagg ggtagtaacg
      841 ccaacagcta tcatgacacc agtccgagta gcaggaacga cagtaagccg tgccagcctg
      901 cataacggtg attatattaa gttgaaggat atccgcttga aagatacagt tctgatttat
      961 aaagcgggag acatcatccc agaagtttcg caagtcgtgt tagataaacg acctaaagac
     1021 agcgaagaat accaattgcc tacacattgt ccagtttgtg gaagtgaact tgtccatttg
     1081 gatgaagaag tagccttacg gtgcatcaat cctaaatgcc ctgctcaaat gaaagaaggt
     1141 ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg ggttggggcc gcgcgtacta
     1201 gaacagatgt atgataaaaa actagtagca gatgttgctg atctttacaa attgacagaa
     1261 gaagagttat tgacgttgga caaaataaaa gaaaaatcag caaataatat tttgacagca
     1321 atcgacaaca gcaaagataa ttcggtcgag cggttgattt tcgggttagg aatccgacat
     1381 gtcggagcga aagcagcaaa aattttagca gaacatttcg gtgatctaga aacattgagt
     1441 aaaagtgact atgagtcgat tattgcgctt gatacgatcg gtgatatcat tgccgacagt
     1501 gtagtcactt atttcagtaa cgaagaagtc catgaattga tgaatgaatt aaaacaagca
     1561 ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat tgcaagaagt cgagtctcct
     1621 tttaaagaaa aaacagtagt ccttactggt aaactaacac gatttactcg tgaagaagca
     1681 aaagagacga tcgaaaatct tggcgggaaa gtgactggta gcgtatcgaa aaaaacggat
     1741 atcgtggtag caggagaaga tgctggcagc aagctgacca aagcacaaga attaggaatc
     1801 gaagtttgga cagaagatca aatggcagat gcattggcta aaagtcgctc agtagaagaa
     1861 tgaaaaatga gttttttcag acaaatttca ataaacaatt tcagacacgg cactcttgtg
     1921 ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa ggggtagtat ggcgattagt
     1981 gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg cgttcgcaga tgaagaactt
     2041 gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg ttgaaatgct ggaagaagta
     2101 gatacagaag gtgttccatt tacgtcgaat gtggttgaaa cagtaaacgt gatgcgacaa
     2161 gatcgggcgc aagctggatg gaaccgcgat gaattattga aaaatgtgcc ggaaacagaa
     2221 gacggcttta tcaaagtacc cgcaatcatc gataatgggg aggctggcgc ataatgacag
     2281 aattacacaa tcgttctcta gaagaactgc atcatatgct tgtatcgaaa gaaatcactg
     2341 tacaagattt gacaaaagca acatttgaac gaatcaaaga aacagagcct gaggtcgaag
     2401 cattcattac attgaacgaa gaaaaagcat tggaacaggc aaaagcttta gatgaaaaag
     2461 ggattgatga aagcaacgtc cttgctggta ttccaattgg tatcaaggat aatatcgtaa
     2521 caaaagatat cttgacaacc gctgcatcta agatccttta caattttgaa ccaatttatg
     2581 atgcaacggt catggataaa atctatcaag cagatatgat ccctgttggt aagctaaaca
     2641 tggatgaatt tgctatgggt ggaagtacag aaacttctta tttcaagaaa acaaaaaatg
     2701 cttgggaccc aacaaaagta cctggtggct cttctggtgg ttctgcagca gcagttgcag
     2761 ctggacaaat tccagtttct ttaggtagtg atactggggg aagtatccgc caaccagcag
     2821 cttttaacgg catcgtaggg atgaaaccta cttatggccg tgtctcacgt tttggtttga
     2881 ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac ccgtacggtg aaagataatg
     2941 cattagcatt gacagctatc agcggttatg acgaaaaaga cggtacttct tcaggcgttt
     3001 cagttcctga ttttgcagag ggactgacag gcgacatcaa aggaatgaag attgctttac
     3061 ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa agaagctgta ttgaaagcag
     3121 cagaaacatt ccgttcatta ggtgctgttg tggaagaagt cagcttgcct cattcaaaat
     3181 acggtgtggc tgtttattat atcattgc
//
LOCUS       DANKHJ010000197         3084 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000197 DANKHJ010000000
VERSION     DANKHJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            144..1076
                     /locus_tag="PW113_003274"
     CDS             144..1076
                     /locus_tag="PW113_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL2527505.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1148..2269
                     /locus_tag="PW113_003275"
     CDS             1148..2269
                     /locus_tag="PW113_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527506.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2351..>3084)
                     /locus_tag="PW113_003276"
     CDS             complement(2351..>3084)
                     /locus_tag="PW113_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2527507.1"
                     /translation="KGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTT
                     IMNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKP
                     DASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSL
                     LILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEE
                     GSHHELLAENGFYAELYHNQFVFES"
BASE COUNT         1224 a          454 c          459 g          947 t
ORIGIN      
        1 ttaatttatt aaaaaaagag aaagaagatt taaatgatga aataaatcaa aagtatgatg
       61 aaacattaaa aaatgtctat tcttcactta tcgtttcaaa tatagaagaa acttcatctt
      121 ctgaaataaa aaataagctt tctttgttaa aattaaaaga aaaggaatat attattgacg
      181 atgctgtttc tgtccctctt attttaaata aacgagagaa aaataattta aagaaaaaaa
      241 tgctcattcc atttgattca gaagtgaaag gattactcaa taaactaact atttctaata
      301 tagactcgac tagggaaaaa atcataaaaa tatttgataa aattaataat ttattcaaga
      361 atgacaatgc tgaattaagg aagggtcttt tagagtttaa acttcaagaa ctagaacttc
      421 attactctta tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac
      481 agatgataga agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata
      541 aagaacaacg tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta
      601 atgcagatgt ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa
      661 aagaattaga agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt
      721 atgtgtatgt aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga
      781 ctagaagatt agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg
      841 aatttgacgt tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc
      901 ataatcattt tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag
      961 tttcactaga cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta
     1021 caaaattagc taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat
     1081 ataaaaagcc ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat
     1141 agaaataatg tggttagaaa aaataaatga caaaaaattt aagtacaccg aacgttatac
     1201 ggatccctta actgaaaaaa agagaaaagt gtccgttact ttatctagca attcacgcca
     1261 agcttggaat caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa
     1321 tgaagaatta ccactaactt ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc
     1381 aactgtaaaa gaaagttcct atcgaacaac tcaggtttat ctatcattaa tttcaaaata
     1441 tataaaagat gatgtacttg taaaaaacgt taatagtaat cttatacaag atatgctaga
     1501 ttactactat tttgaaaaaa atttatcata taattatgta atacatttaa aaacttttac
     1561 aggaatgatt tttaaattcg ccaaccgtcg atatggatta aaatataatc ccgtaaatgg
     1621 ggtttcttta gcaagaaaac ctaagacaaa cgaagaaata aaaaaagaaa aagatggtta
     1681 tttatcaaaa gaagaagttc aaatgattgc tagtaaacaa aaaagttcac atcaacagtc
     1741 acgttattct ttaattacta aatttttgtt tttaactgga ctacgttatg gtgaattgat
     1801 atctttgact gaatctgatt atgatggtaa taaaattacg gtttctggta cttatgatta
     1861 cgagttaaaa attaaaacaa caactaaaaa tacaggttct tatagaacca ttgaattatc
     1921 aagtaatgct aaagaaatca tagatcaatt aattgaagaa aacaaattaa ttaagaataa
     1981 taaagataaa tacattttca tctctaaaaa tggtaacccg atatctattc aagcatataa
     2041 tcaatcttta agagcagtat ccaaagaatt aaacctagat aaaaaagttt cttctcacat
     2101 gttaaggcat tcccacattt ctctacttac agaattagga atcccattaa aagccattat
     2161 ggatcgtgta ggacatgaag attcaaaaac aacattaaag atttatactc atacaactaa
     2221 aaatatgcaa aatcaactag tagaaaaact aggaaaaata gaaatttaaa ttgccccttt
     2281 tctgcccctt ataaaaacaa acaacataaa atatataaaa agaaccccgt tataacgagg
     2341 ttcttttaat ttaactttca aatacaaatt gattatgata aagttccgca taaaatccat
     2401 tttctgctaa taactcgtga tgactgcctt cttcaatgac ttccccatca cgcaagacaa
     2461 cgattctatc tgcattcaag attgttttca gccggtgagc aatcacaaag ctggttcttc
     2521 ctttgattgc ttcatccatt gctttttgga tccttgcttc tgttaccgtg tctacattac
     2581 ttgttgcttc atccaaaatc aataaagatg gatttgtgat gatcgttcga gcaatactga
     2641 tcaactgctt ttgacctgta ctgaataagt tattttcttc ggatatctct gtatcataac
     2701 ctttttctaa cgtcatgatg aagtcatgaa tattcgcttg ttttgctgca ttgatgactt
     2761 cttcatctga agcgtccggt ttgccgaatg tgatattgtc tttgatcgtt ccggagaaca
     2821 atacagattc ttgcaaaacg attcctacat gtctacgtaa tgcatcaaga tcataatctc
     2881 ggatatctat tccatcgatg actactgaac catcattgac atcgtaaaaa cggtttagca
     2941 gattcatgat cgtcgttttc cccgatcctg ttggaccaac aagagcaacc atttctcctt
     3001 tgtccacatt gatcgagaca tctttcaaga ctagtttttc tggagaataa ccaaaatcta
     3061 cgtgctgcaa cgcaagacct tttt
//
LOCUS       DANKHJ010000198         3049 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000198 DANKHJ010000000
VERSION     DANKHJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..605)
                     /locus_tag="PW113_003277"
     CDS             complement(<1..605)
                     /locus_tag="PW113_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2527508.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHL"
     gene            complement(772..1122)
                     /locus_tag="PW113_003278"
     CDS             complement(772..1122)
                     /locus_tag="PW113_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2527509.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            complement(1122..1442)
                     /locus_tag="PW113_003279"
     CDS             complement(1122..1442)
                     /locus_tag="PW113_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2527510.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            complement(1739..>3049)
                     /gene="liaX"
                     /locus_tag="PW113_003280"
     CDS             complement(1739..>3049)
                     /gene="liaX"
                     /locus_tag="PW113_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2527511.1"
                     /translation="KILDELATEANKTSARLDEVNAQFADNKAARNEKQEALMQLNTK
                     EELGELTEEELAQRQTLEAEIKELEASGDTLLEEQLKLEAELKDIRKNQWSEKKETFT
                     DKFELPDDWKDQATDTLNQVGEKMSEAGSQLGKFLKKTFQTVSETVNDNMEWKDVSLR
                     VPGIATTKFEHEFYYEAPAASILDIKAANGNVTLKTWDSNDVKVEAKIKLYGKMGAEP
                     FEAFSERSQIEVNEDHISFQIPNKRVRADLVFYLPKRVYDHAAIKLLNGNIMIETLEA
                     KDIYTKSTNGNIIVDQLTATMLEVEGVNGNIDIRNGNILDSIIETVNGTVTFGATAEN
                     LSVSLVNGDVRLTIKEDNLKKVEASSVNGNVKVALPDGIGLEGHAKTSLGSINSRLSN
                     YEVVREKKERTNQMLQFRRVSDGEIAQVQLSTTTGSIYLKDTDK"
BASE COUNT          848 a          660 c          504 g         1037 t
ORIGIN      
        1 agatgttcca ggattttagg cggttctccg atcaccgtta attcatcttg ctgataaaca
       61 tctactcgat cgtctgcgat caatcgatgg ccgcgtttga tcaactccaa cgctgtttca
      121 ctttttccta tcccactgtc accttgaatc agtacaccta atccgtaaac atccactaaa
      181 acaccgtgga cacttgttcg aggggcaagg cgaccatcta aataactgga taattctcct
      241 aataaacgag aagttgagat gggagaacgc agtacagcta tcccattttt atttgctgcc
      301 tggatcattt cttctggagc tggtaatcct cttgaaataa taaatgcggg catatcttca
      361 ctacataatc ttctcatgat gatcaaacgc tcttctggga tcatccgttc cgcaaaactg
      421 atttctttac tgccaaatag ctgtaaacga ttatgggaat aataattaaa ataaccagtc
      481 aactctagtc ctgggcgtga gatgtcccca gttacgatga cacggtctaa gcttttctcg
      541 tcgcctgtca cgatttccaa agataatttt ttcactaatt ggctaacttt tacaacttct
      601 gacattcatg ctacctcatt tctattagag tgttctctat cattttatca ttgtctagcc
      661 aaaagtacta ccaattaaaa aaaacgtttt tatttaatta ccaatgtaat agaaaacgca
      721 ttcctgattc taaataaaaa aactgcgccg atagttcggc acagctttct tttattttct
      781 actgttatgt tcgctgacaa ttacattgac aagcgacatc aagatagcta ctaacaacgc
      841 tgcaccgaat gaagaaaaac taaaatgttc tgctcccaca aaaaaggagg tcattttaag
      901 aatcgctgca ttgataacaa acgtaaacaa tcccaatgtg actaatgtaa agggcaggga
      961 cagtatcgtc aaaatcggtt tgacaaacat attcaacaca gacaatacga agctggcaag
     1021 aacggccgtc caaatactgc tcacatggac catcgcagga aaaagaacag ccaatgatat
     1081 gaatgttaac gtattaacta aaatacgttg aaagtacgac attaaaagtc actccattcg
     1141 tcatcatcga ttttttccgc ttctttgcgt ttttttgttt gttcacgagt ttgttcacga
     1201 taattgtttt tttcataata tgcattgtta tgtccatagt ttgttcgtgc agaacgcgca
     1261 aatgggataa tcaaagctaa taaaatgtat aaaataattg gacttccaaa agcaactaag
     1321 ctcaggaata cataaagcac acggataaca gtcggatcaa tcccgatgta ttctcctatt
     1381 cctgctaaag tccctgtcaa aacaacattt ttattagatt ttgtcaactt ttttttcatc
     1441 atgtctcacc tctgttctac tattttaaat gaagaaaatc gttgtgacaa ccttcttctt
     1501 aatcggtgtc tttctgcaaa attctcggtc acaaattacc atattcataa aacgtgacga
     1561 gctttttctg aaattattcc ttgtgactcg gcgataattg gcaggttcat aatctcttgg
     1621 taaacggtgt tcaagagata gactatcggc aataaggcac aattttgtaa aataggggaa
     1681 gctattttac aaaatcgctt cttattgctc aaggctgaac atctctttca caacctcttt
     1741 atttatctgt atcttttaaa tagatacttc ctgttgtcgt tgaaagttgc acttgcgcga
     1801 tttcaccatc tgaaacacga cggaattgca acatttggtt tgtccgttct tttttctcac
     1861 gaactacttc gtaattggac agacggctgt tgatacttcc aagacttgtt ttagcatgtc
     1921 cttccaaacc gatgccatct ggtaaagcga ctttgacatt tccgttcact gaacttgctt
     1981 ctactttttt caaattatct tctttaatcg taagacgtac atcaccattt accaaagaca
     2041 cagaaaggtt ttctgctgtc gcaccaaatg tcactgtacc atttactgtt tcgataatag
     2101 aatctaagat gttcccgttt cgaatatcga tattgccgtt tacaccttct acttccagca
     2161 tcgttgcagt taattggtcc acgatgatgt taccattcgt agattttgta taaatatctt
     2221 tagcttccaa tgtttcaatc atgatgttgc catttagaag tttaatagct gcgtggtcgt
     2281 atactcgttt aggtaagtag aaaacaaggt ctgcacgcac acgtttattt ggaatttgga
     2341 aggagatatg atcttcatta acctcaatct ggctccgttc agagaatgct tcaaatggtt
     2401 cagcacccat ttttccgtaa agtttgatct tagcttcaac tttcacatcg ttgctgtccc
     2461 acgtttttaa cgtcacgttt ccgtttgctg ccttgatatc aaggatactt gctgcaggag
     2521 cttcatagta aaattcatgc tcaaatttcg ttgtcgcaat cccagggaca cgtaagctga
     2581 catctttcca ttccatgttg tcattcactg tttctgaaac agtttggaat gttttcttca
     2641 agaatttgcc taactgtgat ccagcttctg acattttttc gcctacttga ttcagtgtat
     2701 ctgttgcttg atctttccaa tcatctggca attcaaactt atcagtaaac gtttcttttt
     2761 tctcagacca ttgattctta cggatatctt ttaattctgc ttctaatttc aattgttctt
     2821 ctaacagtgt atcgccacta gcttctaatt ctttgatttc tgcttctaac gtctgacgct
     2881 gtgctaattc ttcttctgtc aactcgccta attcttcttt tgtattcagc tgcatcaatg
     2941 cttcctgttt ctcattgcgt gctgccttat tgtcagcgaa ttgagcattt acttcatcta
     3001 gacgcgccga agttttattc gcttcagttg ctagttcatc taagatctt
//
LOCUS       DANKHJ010000199         3001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000199 DANKHJ010000000
VERSION     DANKHJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            4..963
                     /locus_tag="PW113_003281"
     CDS             4..963
                     /locus_tag="PW113_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2527512.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="PW113_003282"
     tRNA            1152..1225
                     /locus_tag="PW113_003282"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1740..2039)
                     /locus_tag="PW113_003283"
     CDS             complement(1740..2039)
                     /locus_tag="PW113_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527513.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2045..2275)
                     /locus_tag="PW113_003284"
     CDS             complement(2045..2275)
                     /locus_tag="PW113_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527514.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2525..2725)
                     /locus_tag="PW113_003285"
     CDS             complement(2525..2725)
                     /locus_tag="PW113_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2527515.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT         1019 a          509 c          519 g          954 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcat aagtatggtg ataaaatccg ttccgggcta cctttttagg tagcctactt
     1741 taatctttgt atctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgcttcgag cgtctagcac atcagtcacg atatcaaaca aaaaatatac gtctttcttc
     1861 attctagttt tcgcagcagg gattttaaag tagttcttat tagaatagta gagattaatt
     1921 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
     1981 ttgacaaatc tattcgaagg atcaaattta aaccataatt gcgtctcatt aaaaagcata
     2041 accattactc cgatctattt ttaaacttag tctctacctc taatatatat cgagttttta
     2101 ttttgccttc agagaatact gtttcttttt ttgcagttac aggttgttta ttttcggaaa
     2161 aagctaatat agctaaaatt gaaacatcca tctggaattt gtctttttcg ttgctttgct
     2221 cataaaaatc tgcatattca tcactaatat tttttctaat aaattcttcc atcataaaga
     2281 tcacctcgaa aagagtatac gaacaaacgt tctttttgta aaggtggaat ttattatact
     2341 aaataaatag gtgaacaagt acttgtgcca aattatgtgc caaaaaaaat cgaatttaat
     2401 aaaacacaaa caaaaagaaa tcctattatg ataacatttc ttataataac aaacacaaca
     2461 gaagacgtgt aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag
     2521 ggggttattt tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
     2581 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc
     2641 catcaataaa cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta
     2701 ggtcgacggt gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg
     2761 cttataccag tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga
     2821 agctttacgt aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata
     2881 gctgatgggt taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg
     2941 ttgtttagcg aaataaagga tctaagccaa tagcttagat cctttattct atgctttata
     3001 t
//
LOCUS       DANKHJ010000200         2935 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000200 DANKHJ010000000
VERSION     DANKHJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(70..756)
                     /gene="ftsE"
                     /locus_tag="PW113_003286"
     CDS             complement(70..756)
                     /gene="ftsE"
                     /locus_tag="PW113_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2527516.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(861..1962)
                     /gene="prfB"
                     /locus_tag="PW113_003287"
     CDS             complement(join(861..1889,1891..1962))
                     /gene="prfB"
                     /locus_tag="PW113_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2527517.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2088..>2935)
                     /locus_tag="PW113_003288"
     CDS             complement(2088..>2935)
                     /locus_tag="PW113_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDL2527518.1"
                     /translation="GEEDAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQ
                     REVIYKQRQEVIMEEKSLSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTL
                     VHEDSITLADIEGKTAQEIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDS
                     KWTDHIDAMDQLRQSIGLRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEI
                     RQNVQREQVSQGTASHSEDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCH
                     GKNAN"
BASE COUNT          712 a          673 c          515 g         1035 t
ORIGIN      
        1 gaagcaactg tcatccagcc gttacgtttc aaactcttga tactttctaa taaatggcgg
       61 aaaaaggttc taatcatcgt atccatattc cccttccgct tgatctcgaa taatgcggcc
      121 gttttcgata gcaattactc ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc
      181 catcacgaca gtagttcctt gggcattgat ccgatctaat aatttcatga tttcccatga
      241 gttttctgga tccagatttc ctgtcggttc atccgcgata agtactttag gcgtattcac
      301 tattgcccga gcaatagaga cacgttgctg ttccccaccg gataattcgc taggaaagac
      361 tcgaactttg tgtttcaaac caacaaggtc cagcacttcc attactcgtt ttttgatatc
      421 ccgaggtttt ctgccgataa cttgcatagc gtacgcaaca ttttcatata ctgtttttct
      481 tggtaataat ttatagtctt ggaacaccac gccgatttct ctgcgtagat aaggaacttc
      541 gcggtttttg attgctatta gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt
      601 ttcttctcga tacatcaatt tgatgaatgt agatttaccg gctccagacg gaccaaccac
      661 atagacaaac tctccttgtt cgatgacaat tgagagattg cggatagcgg tcgtaccatt
      721 tgagtacttc ttcattacat ccttcatttc aatcatggcg ttctctccat atctttttca
      781 gtctttagct attatagcat ggcaatgttt caacatgctt gcaaaatctt acaattttat
      841 ttcaatttaa caatgggttc ttattgtaga tgcattttta aatagccgtc aataaataaa
      901 tcaaggtcac cgtccataac tgcttggaca tttcctgtct ctacgtttgt ccgatggtct
      961 tttaccatag aatatggatg gaaaacataa gaacggatct gtgaacccca gccgatttct
     1021 ttttgttctc ctcgaagtgc agctgcttct tgttctttct tttccacttc taattgataa
     1081 agcttagctt ttaacatccc catcgcttgc tcacggtttt tcaattgtga acgttgtgcc
     1141 tgactagcta ctactgttcc tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta
     1201 ttgatgtgct gaccacctgc accacttgct cggtacgtat cgattttcaa atcatctgga
     1261 ttgatttcta tatcgatcgt ttcatcaagc tctggcatca catctaccga acaaaaagaa
     1321 gtatgacgtc gtttcgctga gtcaaaaggc gagatacgta caaggcgatg aacacctttt
     1381 tctgatttca gataaccaaa tgcgttgtgt cctttgatca gtagggtcac actcttgatt
     1441 cctgcttcat cacctgcttg ataatctagt gtctctactt gatacccatg ctgctctgcc
     1501 cagcgtgtat acatacgtaa aagcatactg ccccaatctt gggactccgt tccgccagcc
     1561 cctggatgca attcaacgat ggcgttgttt cgatcataag gaccatctaa caacatagat
     1621 aattcatatg tcttcatttt ttcatctagc gcattgatac gtgtttctgc ttcttcttgc
     1681 atggctgcat caggttcttc ctgcaacatc tccaccatca attcgatttc ttccagttct
     1741 tcagcaagag aatggaattg atcataagtt tctttatttg cattgttttc actgatcact
     1801 ttttgagcag cttcgctatc atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc
     1861 gcaatgtctt cttccagctg gtctaagtca aagagacctc ctgaagcttg tgaccttttc
     1921 ctgcatttct gtcaggagat ttcgaatttc ggatatttcc atgttttttc cttctttcac
     1981 tatacttttt tcattatatc tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga
     2041 cgaattctta aaaaaagaat ttgtctcacc aatcctgctg tcagaaatta gtttgcattt
     2101 tttccatgac agttcttata ttttttcccg cttccacaag gacaaggatc atttcttccg
     2161 accttgttca catggattgg ctgttttttc gcgctagtat tactattttc tttggcatct
     2221 ccgtcttctg agtgagaagc tgttccttga gaaacctgtt cacgctgtac gttttgacgg
     2281 atctcagctt tcataaagat tcgagtgact tcatactcga tggcgcctac catatcttca
     2341 aacatcttat agccttctgt ttgatattcg actaatggat tgttctgtcc ataagcacgt
     2401 aagccgattg actgacgtaa ttgatccatt gcatcaatat ggtctgtcca tttagagtcc
     2461 acaacacgca agatcacgac tttttcaaat tccaacagtt gctctggacc atttagttga
     2521 gccgctttgg tattaaatac ttcttgtgct cgttcgttta gatactcttt gatctcctga
     2581 gctgtttttc cttcgatatc tgcaagagta atactatctt catggacaag agtactgccg
     2641 gcaaaatcga tgatactttc caaattccaa ttttctttct ctaattgtgt atgcgcatct
     2701 actacacgac tgattgtccg tttgaccatg ttcattagtg gcttggataa gcttttttct
     2761 tccatgatta cttcttgtcg ctgtttatag attacttcac gttgttcacg catcacatca
     2821 tcgtattgta agacgttttt acgtgtgtca tagttatttc cttctacacg tttttgtgca
     2881 gattctactt gtttcgtcag catcttgctt tggataacag catcttcctc accga
//
LOCUS       DANKHJ010000201         2930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000201 DANKHJ010000000
VERSION     DANKHJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1625)
                     /gene="brxC"
                     /locus_tag="PW113_003289"
     CDS             complement(<1..1625)
                     /gene="brxC"
                     /locus_tag="PW113_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL2527519.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVR"
     gene            complement(1650..2225)
                     /locus_tag="PW113_003290"
     CDS             complement(1650..2225)
                     /locus_tag="PW113_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL2527520.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(2225..2824)
                     /locus_tag="PW113_003291"
     CDS             complement(2225..2824)
                     /locus_tag="PW113_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL2527521.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT          895 a          575 c          385 g         1075 t
ORIGIN      
        1 cgcacaaagt tttccttaat taaaaggtcc aatgcttctt ctactcgttt tttagtttcc
       61 aggcggtctt cgttgatatt tcttatcatc aagctcgtaa tattttcaac atttggtttg
      121 atggcttgat caacatattt aatcatgaat aaaattcgta agacatttac gttaaagtta
      181 tctatatcag ttggtctttg aatatgagaa ttatttgtag cttgtgtaat cactcggcta
      241 attgctgtat caatccaacg ttctaacgag taataaaact gatctaaaga aaccaaactg
      301 gttgtatcat tttccttatt tcgttcagcg gcttctttaa acattgctag taatgaacgt
      361 tcaccagacg ataagtttga tccttgatag ctgtgattac gaatctcggt tagaatttgc
      421 cccaataaat tgaattgata gggaataaat ggataaactt ctgcgtaatt ttcagcatct
      481 tggaagagct ttaattcagg actatcaaca aagagtaatt tatttttcaa ttctgtttga
      541 acagtttcaa attgtaaggc taaagaatca ttggcaaact cttttttctt caaaatacgt
      601 tctttaatta cttcatcggc atttgctgaa gataaactca cagttgtggc aaaacgatcc
      661 ttgatcttag aaaaatcatt atcagaattg ttatcaaagt ttgttacgtg atcaatcgct
      721 tcctgacttg tcactaatac ccaagcctta ccttcacaaa ttcgacccaa atcttccaca
      781 acggtttgta actgcaaagt caactcacga ttaccgccga tatattgtcc gacctcatcg
      841 actagaaagg caatatgatg gtctgcttct ttggaatcaa gatagctttt aactagattt
      901 gcaaagtcat caatgcttat taaataattt cctgatgctt tttctaaaaa attatcaata
      961 gcggcttcac tcataaaatc gattttaagt gccgcttctt tgatatcatc ttggatccaa
     1021 tcaaactcat ggcgctcttc ttgccatttt gccccattaa tctcttcaaa agcagctttg
     1081 aatgcatcaa acttttcttc tttaattaac cgacgttcaa aatctgctag atgtggaatc
     1141 gtgcttaatc cttgcatttc gttaaatacc tttaaaaaaa cattcacgat tccgtttttg
     1201 tcttttttac cacctgcatc actttttgaa tcaatattaa acagaataac atctgtattt
     1261 atttgtgagc accgttccat cgttgccaaa gtcagtggat ctttgatttt cccatcttct
     1321 ttgaagtaat caatggcttt cttgtcattt atttctcgat ttgcgagcaa gtaagaaata
     1381 atttttaaga aatgagattt acctgaacca aagaacccct gaatccaaac acccatttta
     1441 tctgttggtg tattgatact ccgttggtag gcatcaaaaa ttgttttaaa gtgtttttgt
     1501 agttctttcg taaccacgta ttcttctaat tcttgttgaa cattttcatc ctttgcttga
     1561 ccgaccttaa taactccttg aatatttcgg tcaattggtt ttgcaaaaat atctttaatt
     1621 agcatttaat ctcctacctt ccatcccaac taaattctaa atgctcgata ataattatca
     1681 tctttattgg tattaagaat tgtcaaactt aattcatcat attcccctgg atacatcatc
     1741 acaactggta cgcgatcaaa tacctgatgc attttattta aaatagagtg cgctctaatt
     1801 aatggatata attcgccaat tcctgtcagg aacacaactg atttttctgg tgtatgggca
     1861 tccacatatt caaaaaatac attttcttcc tcaggtaaat ccattttcaa taaacgacca
     1921 atgctttcca ctaatttttg taaaccacgt cgttcttcca tcttaatcgc tttttcagtg
     1981 aaaccgcgtt tttctagata ctctaacata atcacataga gattataaac tttcacttca
     2041 taattatcac caacagatgt tccagatata tcattaatat aatctcttac aattaactcg
     2101 tctttcggat cgtagtcaaa gatataatag cctacttcat tacctaaacc acgattcaat
     2161 aaaaaatctt ctttttgaat tacttcattc aacttttcca aatgctcttg aatcgataat
     2221 gacattataa aattcctccc agtgctagta caatatcttt acgattcttg tcaactaacc
     2281 attgaatcaa tcgtttatct aataatatcg gagtcattcg tccatattct ttttcccctc
     2341 tgactaatcc tgtttcaatt agattcgttt gataagcggc aataagccgt ttaatcgtat
     2401 aatctttcca ttttgaaata tcttctgaac ttctccgctt ctctgtaaaa aaagaacggt
     2461 aatcaactgg tgttaattgc aaatttcctt tacgtagatt ttcagcatat acctcagtca
     2521 taaattcatt aaaaagatca tcaatcataa ggatggaaat caaattcact aacttttgat
     2581 tatctatatt taactgattg aaaagttgca atccagcttc atccagttga ctaactctcc
     2641 ttttcacgtt ctgatagatt tctttttgac ggttactgct cactgcaaaa aaaatatttt
     2701 catgttcatt tttctcttta atttctatat ctgtatatcc ttctagtcgt aattgaatat
     2761 ataacttgaa ctctgaaaac caaaagcgtt tagaaactag accagcagaa tattttttat
     2821 tcaatagatt gcctcctaat catctataat tcaataagta aacaagtgtt ttttgctcat
     2881 cgttagcatt cattttttag tatctactat acaatacata tttattatag
//
LOCUS       DANKHJ010000202         2927 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000202 DANKHJ010000000
VERSION     DANKHJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(45..1364)
                     /locus_tag="PW113_003292"
     CDS             complement(45..1364)
                     /locus_tag="PW113_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2527522.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="PW113_003293"
     CDS             complement(1361..2014)
                     /locus_tag="PW113_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2527523.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2927
                     /locus_tag="PW113_003294"
     CDS             2302..>2927
                     /locus_tag="PW113_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527524.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          959 a          477 c          322 g         1169 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgt attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgcgctttc ctagaaa
//
LOCUS       DANKHJ010000203         2888 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000203 DANKHJ010000000
VERSION     DANKHJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            438..1397
                     /locus_tag="PW113_003295"
     CDS             438..1397
                     /locus_tag="PW113_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2527525.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1384..1989)
                     /locus_tag="PW113_003296"
     CDS             complement(1384..1989)
                     /locus_tag="PW113_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527526.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2229..>2888)
                     /locus_tag="PW113_003297"
     CDS             complement(2229..>2888)
                     /locus_tag="PW113_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527527.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          929 a          496 c          454 g         1009 t
ORIGIN      
        1 gcttttcact aattacaaaa tatgtgatat aggaagttta gttattgttc tcataatgcc
       61 atcctgtttt tagattatct agtaattttt ttgttattta aaattaggag cagtgccaat
      121 tgttttaatt ttaaaggctt tttttatatc ttctaaatta gaagatattt gtatatcaat
      181 atggaatata ttaccttttt tgtatttttt tatgttatct ttagctaaaa catcaaatac
      241 atgtccttga aattgtatcg aggcagctgg atttagttta aattgaggaa actttaccac
      301 agctgattca aaacacttgt cacatttttt tattttttta ccttcgccga ttgtaaaatt
      361 aagctagaca actaaaaaag tcatttgtgc tacactcaaa atagttccgt cgaaagaatt
      421 ataaggagtg agtacaaatg acctataccc atcttacacc aactgaactc gtaatgatag
      481 aagcttattt caatcaatca cactctgttt caaaagtagc ccatctggta caacgttctc
      541 gacaaaccat ttataatgtc tatcgttttc ttaaatctgg tggaagcgca ataagctact
      601 atgaacaata taagaaaaac aaacgtaact gcggaagacg tccgattgtg ttaccagatg
      661 ggcaacaaga atacattcaa aaaaaggtgg ctcaagggtg gactccagat gtccttattg
      721 gtcgagcaga gtttcctatt gcttgttcgg tacgtacact atatcggatg tttaaagata
      781 aaacctttga ccctcacgat ttaccaatga aagggaaaag aaaaccaaac ggacataaag
      841 aaaaaagagg aaaacaagct tttcgccgtt cgattcagga tagaacaact gattatatac
      901 agttcaatca tgagtttggt cacctagaag gtgacaccat tgtcggtgga aaacataaaa
      961 gtgcggtgat cacgttagtt gagcgattat ctaaagtgat catcaccttg aaacccgctg
     1021 gtaggcaagc tatagatatt gagaatagac tgaatcaatg gtttcaatct gtaccgaaac
     1081 acttgtttaa atccatcaca tttgattgtg gaaaagagtt ctcgaattgg aaaaagatta
     1141 gtaataccaa tgatatttcg atttatttcg ctgatccagg aacaccttct caacgaggac
     1201 taaatgaaca ttcgaatggc ttattacgta aagatggctt atataaagct atggatttta
     1261 ataaggtatc cgaaaccttt atccaatcgg tggcatcaaa acgaaatcat attcctagaa
     1321 aatcattaca ctatcgcaca ccattggaag tatttttgag ttacgtaggt aaagatgatt
     1381 tgtctagctt aatttgacaa acgaaacttt gtaaaaatat tcgattgaat attctatttg
     1441 gcacgactca cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc
     1501 aaaatctaaa ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa
     1561 atttatcttt ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc
     1621 tctatcaaaa tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat
     1681 caaacctata taatcatgaa tgctggcaaa aatttttttt aagtcaagtt ctaaatcttc
     1741 ttcgaaaaca catttaattt ctcctactga taattgatac tctatattac atgacttaat
     1801 tagtttttta gatcccccct ccagataggc atatgtttca ataattatgt tctttttatt
     1861 gcttggcaaa tttccgttat ttattgcata aattaaaaat gactgtctat ttagcccata
     1921 atctaataca acttcttcat actcatattt ctttaaatat ttaatatgaa aatatgctga
     1981 attaatcaaa actcgagtgt tactgttgtt atttattgtt gttctattgt gtataaaatt
     2041 gtataggttg tcatattttc ttggtttagt gaactactca ccacttagct aaacctaacg
     2101 gtttcttacg tttgaagtgg gagcttcttg ggaagagagc atacttgata gcgtattact
     2161 ttactaacag aggtgcctct tatttaccaa gctatccccg tagttcctac ggttcttgtt
     2221 tatttgatct aagccaatcc catagagtgt agccttagac cttcggtcag gatattgata
     2281 ctagcgttga tatctcggtc atgatgagta tgacaaatag ggcaagtcca ttttcgaatt
     2341 tcgagagatt tcttgccgtc tttatgtcca caattcgaac agatttggct agatggaaac
     2401 cacttatcta ctttgatgat tttccgaccg taccagtcag ccttgtattg taatttagac
     2461 acaaaactag accaagaaac atccgaaata ctttttgcta gtttatgatt acgcaacatg
     2521 ccttttgtgt ttaagtcttc aatacagatg atatcgtgat ttttgataat ttctgtactc
     2581 aacttattca gaaaatcagt acgttgattc attacttttt catgcaatcg tgctacttta
     2641 cgtttttgtg tttgataatt tctagcttca aacagattga tacctttttg tttagctaat
     2701 aaggcacgtc ttgacaactt acgttgttca cgtttgagtt tcttttccat tttggacgta
     2761 aatttattat tatcaaattt ttgaccggta gaaagaattg caaagtctat aatacctagg
     2821 tcaataccaa cagcagaatt agatttaggt agttcgcgaa cttcttcttt gcataacaaa
     2881 gaaatata
//
LOCUS       DANKHJ010000204         2861 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000204 DANKHJ010000000
VERSION     DANKHJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..290)
                     /locus_tag="PW113_003298"
     CDS             complement(<1..290)
                     /locus_tag="PW113_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL2527528.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKT"
     gene            complement(449..679)
                     /locus_tag="PW113_003299"
     CDS             complement(449..679)
                     /locus_tag="PW113_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527529.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            complement(1426..1893)
                     /locus_tag="PW113_003300"
     CDS             complement(1426..1893)
                     /locus_tag="PW113_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL2527530.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(1968..2408)
                     /locus_tag="PW113_003301"
     CDS             complement(1968..2408)
                     /locus_tag="PW113_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2527531.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(2438..2731)
                     /locus_tag="PW113_003302"
     CDS             complement(2438..2731)
                     /locus_tag="PW113_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527532.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
BASE COUNT          812 a          602 c          453 g          994 t
ORIGIN      
        1 gttttcaaag cgtttttatt acccactggc ggagaagctc ggctatttcc tttgttacct
       61 ttagcatttt gattaccaat aggagcgcca ccttgattag taacgttact tttgccattg
      121 gtaacgttac taatcaattc agcgctccat ttgtcttgcg atttccattt tctaacctga
      181 gaatctgaaa catttaattc agatgcaatt tcctttaact gcttttctcc gttggattct
      241 aaccaaattc ttttggcttc atcacgtctt ggatcacgtt gtcttgccat tcaatacaca
      301 ccacctcaca ttctgtttag gttgagtttt gttttctaat tttcaatatc ttttagcatt
      361 aaatcagctt caatcaatat ctttaaatcg gaaactttgt ctaacttgat ttgtcctgat
      421 tggagatttt taagccattt cttgcattct aatccctcaa attcttgtta atattatttt
      481 gaatgtttgg ttcatcatag aaaccatgac cgcaataaac gagcttgcac gcatcaatct
      541 cacatggttc tgcttctctc cccatttcga caatagagta cttccctttg atttgtacag
      601 aacgcacaac acgcactgaa caatcatcaa tggttcgagg atattcatta gttagcgata
      661 cataccagta gtttctcatt atgtagcctc ctttatgtaa agaaaaagac cactcaaaga
      721 gtgatctaat atgtaatgca caggcaggga cgtttccgat cctgtgcttg agacatttga
      781 cgattcattt gtaccgaatc ccaaatctca atctaaccta accagttatg taatagcaac
      841 ctacacgatg cacaaaacgc gtacgaaatt gcgcacccct atatttttaa accgccgatg
      901 cctcggttgc caaagtcact ggcaaggaat cgaaccttgc atggttgccg aagcattgac
      961 ctagcacaca tgcttagcgt ctaccctttc cgccacagtg acactataaa attattcttg
     1021 gctgctacta ttttttattt tgcccatttt taaatccaat catatataca ttaagacaaa
     1081 gcgcaaaaat tgaaattatt aatggaatca tttctcttca cttaccttta gttatcgtgt
     1141 gaataattaa aaaaacaata gacagcaacg gatgatagat aataagaaca atttagaagg
     1201 agttgaaatt cacatcctta ttcttaatat ttccgttgct gcctatcgaa gcttaattaa
     1261 acgatgaggg agatttcctc ccttacattt tattttgtcg atcctgtttc ctaatttttc
     1321 gacactatca taatacaacg ttgaataggt aagtgattgg tataaaaaag gtataaaatg
     1381 gaaaccaaat tggtaataaa agggtataaa aagtgtaaaa actggctact tgaaagcaac
     1441 cagttctaac gatgaagcaa attggatgat aattctgttt gattctactt taaccgattc
     1501 ttcgctagta ttattccttt gagcagttac ataaatgggc agaccattga tataacgatc
     1561 atagaatatc ttcttgcgcc tttcagtcac atcaggctta tgcggatgct gtatcgctga
     1621 atagcctcga acaaacaatt tatgcagata ctcaaattct tcctgtgctt cttctttatc
     1681 gatcagcatt ctttctgctt caaatatatg atcagctgta gaaggtggaa ccaaggaata
     1741 agatgctgtc acttttggtt ctcgatgttg ccctactcta catctagctg atagatatgc
     1801 tgaaagaaac acagcgacat tatgttttgt gcgttccata tcaacatctt tcgcgcttgg
     1861 tgtctcatat ttctttacat cgaaaagtac catcctctga ttcccccaat tatgatataa
     1921 tacttatgtc ggaaatatta ttcatagtcg gaggaatccg acttttttta ttttgtacgt
     1981 gaaatgagtt ctcctgattt atacgcctca gcaaattcaa ccaaagctac tgccttcatt
     2041 ctctcgatag ttctatcgga atatcctaat ttttctccta tttgatacat tgacatctta
     2101 ttaggaaaac aataactata ataaagtacc tggaaatgga ttctacttag ccgtgttaat
     2161 gcttcgataa ccgaatctcg agtacttatc gcttcaatga tttcttcgtt ttgactgtca
     2221 attgtgaatt tcaaattttt ggtaacagtc atcacggaaa agtctatttt cactggtccg
     2281 accaaacgtt ctaaatttct gtatcttttt agtaatcgtc tagcattttt ccttgtttgc
     2341 ttaatatcta tattctctaa aatgttcaca attgcctctt ctccttccag ttgtggaata
     2401 acgctcattg ttgtaggata gttgtaaaaa agcaaaatta agattttata acttccatgt
     2461 ccaccaatct caccactgct aaattctctt tgcttttcgc taaccactta tcacattcca
     2521 tcgtgttttc aatgcgaatg attgctgagt gattatagac gtgttctaca tatccacgaa
     2581 atggatagat gaatccttct gcttcgcagc gaaccatgtc accgactttg aattttggtt
     2641 tcttacgtgt tttaggattc tttgtcggca tatctagcat taaaccgccg atgccgtggc
     2701 cgctggagta aaatccgtct tttagtttca ttccgcttcc tcctctttag ctgtcaatta
     2761 cttactcgca agtcactaaa atggctactt catcgataaa gtttccgtct tcgtcttcca
     2821 ttccttgctg aatgctaatg tcggtaatct gcttaaaaaa t
//
LOCUS       DANKHJ010000205         2859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000205 DANKHJ010000000
VERSION     DANKHJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..667
                     /locus_tag="PW113_003303"
     CDS             <1..667
                     /locus_tag="PW113_003303"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2527533.1"
                     /translation="DSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFD
                     ELPQYTIIAASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISH
                     IIIGIGLNFSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKA
                     LDFYKEKSFVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSI
                     RTQ"
     gene            complement(984..1508)
                     /locus_tag="PW113_003304"
     CDS             complement(984..1508)
                     /locus_tag="PW113_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2527534.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            1795..>2859
                     /gene="scm"
                     /locus_tag="PW113_003305"
     CDS             1795..>2859
                     /gene="scm"
                     /locus_tag="PW113_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2527535.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          924 a          574 c          520 g          841 t
ORIGIN      
        1 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
       61 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
      121 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
      181 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
      241 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
      301 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
      361 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
      421 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
      481 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
      541 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
      601 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
      661 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
      721 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
      781 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
      841 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
      901 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
      961 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
     1021 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
     1081 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
     1141 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
     1201 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
     1261 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
     1321 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
     1381 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
     1441 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
     1501 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
     1561 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
     1621 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
     1681 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
     1741 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
     1801 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
     1861 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
     1921 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
     1981 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
     2041 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
     2101 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
     2161 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
     2221 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
     2281 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
     2341 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
     2401 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
     2461 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
     2521 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
     2581 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
     2641 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
     2701 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
     2761 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
     2821 acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DANKHJ010000206         2843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000206 DANKHJ010000000
VERSION     DANKHJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..99
                     /locus_tag="PW113_003306"
     CDS             <1..99
                     /locus_tag="PW113_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDL2527536.1"
                     /translation="AHIRFVPTVILGKEIFDESISIEELKELIQTK"
     gene            complement(798..1363)
                     /locus_tag="PW113_003307"
                     /pseudo
     CDS             complement(798..1363)
                     /locus_tag="PW113_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1542..2198)
                     /locus_tag="PW113_003308"
     CDS             complement(1542..2198)
                     /locus_tag="PW113_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527537.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(2766..>2843)
                     /locus_tag="PW113_003309"
     CDS             complement(2766..>2843)
                     /locus_tag="PW113_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527538.1"
                     /translation="ISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT          912 a          596 c          366 g          969 t
ORIGIN      
        1 gcacatattc gatttgttcc gactgtcatc ttgggtaaag aaatctttga tgaatctatt
       61 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtaggaca
      121 aaagtaaaga acacttttgt ctcacgctct aaacacctat aaacggcgga agcagaagca
      181 actccttctt atttcttgga gttaaacgct tctgtcccag cctccttatt ccgaataaat
      241 ggtgtccaaa agcttgcatc tgccagatta tctcatttct tatgacaagc tgagcaaatt
      301 atctccacca aataatttat tcatattttt gacttgcgca tactaactaa ttcttaaatt
      361 ttcatacaaa atttctcaaa ttcccttaac gagtaatatt tatgtacggt gattcacact
      421 agtatcccaa tcacttttta agttatcccg atttcaattc tacttcaaaa atagttgctt
      481 ttatgtatgc tgttagttga ttagatggtc gtttgttgag ctaggtcgtt tattttaaac
      541 aagttgtttc tttagcttat acctatctcg tctaccaaac gcaagatggc aaacacaaat
      601 gcatacgcat ctgtatttgc cactttgatt cgtataaagc taacaactcc taactcctct
      661 catttaatag ttgctctctt ttttattcag atacattttt gctgatcaca gcctagtatt
      721 tcaatggttt tgaacactaa accaaattgt tccattacaa cattcactgg ctgatgaaat
      781 ggattaatag atttcgatta ttcaaagcga ttttctattg gtggattgtg ttcccaacta
      841 acaagacgga ttccaaagtc attatataac tcttcaagag ttaacccttt cctgttccca
      901 atagatgtat ctctcccacg ctcgatataa aatatggagt ttcgaatgcc aagacctggc
      961 aaatctttcc ttgttacagt tacactgcta ctaacttccc catataaaat ccagctcctc
     1021 caaaatgata atatattttt cgaccatcat ctaactcatg taacaataat tgttgtgccc
     1081 tcttgtcact cgccaaatgc aaacgccacg tgcgatacca tgccatgcta cctgcatctg
     1141 cagaaaatcc atcaaataat gcaacaattg tatcttctat cacaatcctt tccccatcta
     1201 cttcatattc taatctaaaa tcaaactccc ctcgggtaat cgcaggtctt ggtggcttag
     1261 gacccaatat tattctactt aaaaccgcat tcgttcccag atgaaacaca gctataacaa
     1321 caataatgat taatattttg tttctatttg ataaatttct cattattcgc aaatcacctc
     1381 tacattctca catactagtt cgtaatactc ctttaatatc aaaatatcta ctgatcgtaa
     1441 taatattcgc tatgaattcc tactactcgt ataagaaagg ccaaatcatc agcttttact
     1501 tgctgatgat ttggcttgtt tctatttatc gtaattgttt tctaatcacc aaaattgtta
     1561 acaatcggtt cagatatttc ccaattcact agccttatat tatattgatt taacaattct
     1621 tcttcattta ttgtattttg atcaagcgga ccaaattgtt ctaataattc tttgtcatgt
     1681 aaaatatccc ttctagtaat gcctctttcc ctttcaatga aagaagcact aggaaaagta
     1741 tgattataac cattcaacct atctcccatg taataattag caggtcctgg aaaataaaat
     1801 atttgcttat tatcactaac ttctaacagc aacactaaat cttctccgct ggctaaacga
     1861 cttgtccatc tacgatatct ccctatgcct tcgttcattc caaatccatc aaattcaact
     1921 attaacgtgt cttctatcac atgtaattct tcattcaatt catactcaag tctaaaagga
     1981 aattctccat atgtaatctc tggcaatggt ggtgctggag ttctccaaat aaatacaata
     2041 actgtgaata gaaaaaatcc aacgatcgat ataaatgtta tgagacctgc tcgaagtaaa
     2101 acccgatata ataaaaatgc tgtatttcgc tttgctatat ctagaaaaat aaaaagtact
     2161 gcaatcataa tgatagtaaa agttgtaaaa aaacgcattt ttgccctccc tttataaaca
     2221 tcgattctca aattctaaac caaatcttgg tagttcaagt tcaggtataa cgtattccaa
     2281 ccatacatct caattactat ttatgaactt atgattatca ctgaataaat cactccatcc
     2341 ccttgttaaa acttctacat taattacacc tggttgtttt cgactagcta atccaatgaa
     2401 cttatcaacc atccttatcc accaaccacg agaatcttga ccagaagtaa gaagagcagt
     2461 tctcaaggat gatcgagcag ttccttccag ttttttaatt aatcaggcca gttatagata
     2521 aatacttgat cttatctata tcaggacttc tatattaagc aaaaatttat gtacaaaact
     2581 aaaatcttca ttcagttgtc tccccgcttt ttatacgatt gtattaaaaa caatagaagc
     2641 ctttccaaat tatgttactc cagtaaagat ggaattatag tttgacggaa gaaaagcaag
     2701 tataactttt aaaaaaaact gatacattcg catctcctgc ttcatgtatc agttttttct
     2761 ttggattaaa ctgcttcttc acgcagtttt tccgcaaatt catttatttt gcgaattcgg
     2821 tgattaattc ccgattttga tat
//
LOCUS       DANKHJ010000207         2815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000207 DANKHJ010000000
VERSION     DANKHJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(91..417)
                     /locus_tag="PW113_003310"
     CDS             complement(91..417)
                     /locus_tag="PW113_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527539.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(429..629)
                     /locus_tag="PW113_003311"
     CDS             complement(429..629)
                     /locus_tag="PW113_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527540.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            752..1354
                     /locus_tag="PW113_003312"
     CDS             752..1354
                     /locus_tag="PW113_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527541.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1409..2575
                     /locus_tag="PW113_003313"
     CDS             1409..2575
                     /locus_tag="PW113_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527542.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          995 a          469 c          416 g          935 t
ORIGIN      
        1 tattattgag tgttcccccg atctgcaaac tgacgggaac actcttttta attttttcta
       61 cttcatcaaa tttaaaaaac gaggtgtttt ttaaatttga tgactcaata tccatttttt
      121 gcattcttct tttgaaaaca actccatttt ttctaataaa ataactggca atcccattct
      181 tttaaatttc aaaaaagttg tatatgatac attcaaatat ttagccatct cttttccttt
      241 tagaaatggt ctttctaatc cggtatcaat tcttgcttga tcaatagcct cttttgtcat
      301 ttcaaaaaca taatttttca attgattcgt ttgttgttta tctaacatta ctgtaattcc
      361 tgcatccatt atttttctcc tctcattttc tatttcttca atcagccaac tcgccaagtt
      421 gtatttattt agattttcaa atcattaagt aaccaattta ccaacttttg ataagtagat
      481 gctctaatac tttctttgct cccattttcg attttgacat atgttttccg atttatacct
      541 atcaattttg ctgcatcgtt tttagtaagt ataagatcgg ctctatgtct tcttaagctt
      601 ctaatgaatt gatcattaag agttatcatt cagcgcctcc tttcaaatta ttcactttgt
      661 ggtttttatc ttaataagca cattttaaac cacaaaacga gtaatatcaa ttaaaatatt
      721 actcaatttg tggttttcat gctataatag tttggaattt actatagggg gcgttacttt
      781 gaatagaata aaattttata ttgattccag taaaaagacc attaaacaaa ttagtacaga
      841 aactggtatt aactatacaa ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa
      901 tactgaaaca tggaatatcc ttgctgatta tttccatgtt cctgtaagtt atttgatggg
      961 attagacgaa acaaattcca tagaacaacc taatcttgat agaataaaaa ctgctcgttt
     1021 agaaaaaaag gacaaaatag tcaagaatat tgccagtctt gactcagatt attttactga
     1081 attatctaat ctatactttt ccatgacttc tttacttgaa gtcaaacttc aagaagatga
     1141 ctccgacttt ctttttttaa aaagtattct agaggactcc tttgaataca cgattctatt
     1201 tgccaaacaa aagaagttta ttaataaagc tttaaactta aatatcgatg aaagaaaaga
     1261 gttagaatct gagttatttg gcaacttaac ccaagtacag gttaacaatc taaaaagaga
     1321 gataacagct cgattaatat caaaacttca ataaaactaa ttattcaatc agccaactcg
     1381 ccaagttatc agattggaga gattagttat gtcaatacaa aagtacacaa caaaaagcgg
     1441 tgcacgatat agtgtccgct tgtatgttcg taaagatgaa aatggaaagc aagaatacta
     1501 tgtaaaaaaa gggtttaaaa ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt
     1561 cgagattgaa gataccggct atcataagcc ttctaatgac ttgtttataa cagtgtatga
     1621 agaatggttg ccttcttata ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga
     1681 ccttttccgc ttgcatatac taccaaaatt cgggaataag tccatttcta agattactcc
     1741 tgttcactgt cagaatgcag tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct
     1801 taagtcttac agctctcaaa tatttgagta tgccattttt gcagagctat tacaccgaaa
     1861 tcctatggca aatacaaatc taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc
     1921 attagaagaa ctaaaagctt tttgggatat tctcaataaa gaagagcctt taaaacatat
     1981 tttaatcttc cagcttttga ttactactgg tatacgtaaa ggagagctag ctgctcttca
     2041 ttggtctgac atttattttg aagaacagct aatgtatgtt agaaagagct atgcaactat
     2101 tcgaaatgaa gagaagaatg caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa
     2161 tatatcttcc gaacgaatat taccaattga tgcacaaact attgaaatgt taaaaatctg
     2221 gaaaaaagaa caagctcgag agcttttgca gtttggcata aatacaaata caaaaaatca
     2281 acttatcttt acctatgtaa atgcagacgg tgacattaat caacctttgc atgcggacta
     2341 ttcaaataat atattgaaac gtttagagaa gaaatataaa tttaagcacg ttactattca
     2401 tggattccgg cacactcacg ctactcttct actagaaggt ggagcaagta tcaaagaaac
     2461 acaagatcga ttgggtcata aaaatgctga aactaccctt aacacttatt ctcatgttac
     2521 tgaaaaagct caacgaaatg cgttagaaaa attcattgct tacactggtt tataatattg
     2581 tcattacctt tctcattacc agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc
     2641 tgaaatcgtt ggtataacaa cgattaacgt tttgaaaact gtgaagcttt acgtgctttc
     2701 ttaagacctg gttttttctt cgtatcattg aataagtttg aaaagccttg atttataaag
     2761 gtttttccgt aaatatattt tgttatattt gattaaattt gaaatgtttg tgttc
//
LOCUS       DANKHJ010000208         2792 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000208 DANKHJ010000000
VERSION     DANKHJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1566
                     /locus_tag="PW113_003314"
     CDS             <1..1566
                     /locus_tag="PW113_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2527543.1"
                     /translation="PGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQS
                     PEFAMEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYK
                     VYGEDGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEE
                     IKAVTIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDF
                     TTVKSPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQI
                     GALFAQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEK
                     IQQFEEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGL
                     QTIFETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELT
                     RTDREGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKV
                     AAYETIIQKMTEE"
     gene            1582..1932
                     /locus_tag="PW113_003315"
     CDS             1582..1932
                     /locus_tag="PW113_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2527544.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2016..>2792)
                     /locus_tag="PW113_003316"
     CDS             complement(2016..>2792)
                     /locus_tag="PW113_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2527545.1"
                     /translation="TMAQYPSVEFNLFRRTFVTDEKTLMQALQDALEEKALVLHTLIN
                     QELIEMTRSFCEEHQLFSFDVMTPPISEIERLTGIKPIRQPGALHLLNENYFKRIKAM
                     EFAVKYDDGKDPRGFLEADVVLLGVSRTSKTPLSLFLANKNLKVANLPLIPQAHIPKQ
                     LWEIDPKKIVGLTNNPDILNNIRKERMRSYGLNPDTAYSDIEKIRAELDFANDLYEKL
                     GCVVINVASLSIEETASLILNALDLEDHSYYGTETTEETD"
BASE COUNT          912 a          556 c          595 g          729 t
ORIGIN      
        1 cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg acttgcacgt
       61 ttcatggata cacaaggcga agaaacaaaa cgccgaggtg tcgctatcgc ttatgactca
      121 cgccatcaat caccagaatt tgccatggaa gcagccaaaa cattagcaca tcacaatatc
      181 ccatcttatg tattcgaaag tctacgtcca acaccagagc tttcttttgc agtccgtcat
      241 ttgaagacgt ttgcgggaat catggtaacc gcatcccaca atccagcagc ttacaacgga
      301 tataaagtct acggagaaga tgggggacaa atgcctcctg cagacgcaga tgcattgacg
      361 aaatatgtcc gtgaagtcag caatccgtta aaagttgaag tattatctga cgaagaagct
      421 gaacatagcg gattgatcac gatcatcgga gaagaagtgg atgccgctta tctagaagaa
      481 atcaaagcag taacgattga tcatgaattg gtggaaacaa tggggaaaga tttgaaactt
      541 gtgtacacac cacttcacgg aacaggaaaa atgttgggcg aacgtgcgtt gaaacaagct
      601 ggattcgaac aattcgtact agttccagaa caagctgtcg cagacccaga tttcactaca
      661 gtcaaatcac caaatccaga agaacactct gcttttgagt atgcgatacg acttggcgag
      721 aaagaaggcg ctgatttgct gattgcaaca gatcctgatg cagaccgttt aggtgcagcc
      781 gttcgtttgc caaatggctc ctatgaagta ttgaccggaa accaaatcgg agcactgttt
      841 gctcaatata ttttggaagc acaccaatcg cgaggcactt tacctgaaaa cgcagcggta
      901 ttgaaatcga tcgtttctag cgaattacct acagcaattg cggaacacta tggggctacc
      961 atgttcaatg tattgacagg attcaaattc attgctgaaa aaattcaaca atttgaagaa
     1021 gatcattccc atacttttat gttcggattc gaagaaagtt acggttattt agttaaacct
     1081 ttcgttcgcg acaaagacgc gatccaagca ttgctcttat tagcagaagt agcagcttac
     1141 tataaaaaac aaggcaaaac gttgtatgat ggcttgcaaa cgatttttga aacctatggc
     1201 tactacgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc
     1261 tctgctttga tggaaaaatt ccgtgaagaa gcaccaacag aatttgccgg agcaaaagtc
     1321 atccaaacag aagacttcaa agagttgacg agaacagacc gtgaaggaaa cgtagaaaaa
     1381 ctaacgacac cgccatctga cgtattgaag tatacgctgg aagaaaatgg ctggatcgct
     1441 atccgtcctt caggaactga gccgaaaatc aaattttata tcggtgtaaa aggaaactct
     1501 tcagaagacg cagctgaaaa agtagccgct tatgaaacga ttatccaaaa gatgacagaa
     1561 gaataaaaaa acttgagact gatggaaaaa gtggtaaact ttagaagtca gtcttttttc
     1621 aagttggagg tgaatggatt gccagacacg caaaaaacaa aagtagagaa agtcagccaa
     1681 ctattcaaaa tgttgagtga ccccactcgt ctcaaaattc tgctgtatct gaaagacggg
     1741 gaacaaaatg tgacagcaat catccaagca gtggaaatgg aacaatctgc tgtatcgcat
     1801 caactgcgtt tattaagaga aaaccatgtc gtcaagtccc atcgtgaggg aaaagcgatt
     1861 ttgtatagtc ttgatgattc tcacgtactg gatattttga accagacact caaacatgtg
     1921 gaacatcaat aacgataggt gcaaatagag acaaactgga ctgtgtcaac gaatcttctt
     1981 cgctgacaca gtccagtttt tttaaagaaa aattgttagt ctgtttcttc ggtggtttca
     2041 gttccataat aactatggtc ttctaggtct aaagcattca aaataagcga agctgtttct
     2101 tcaatagata aagaagcgac attgattact acgcagccca atttttcata aagatcattc
     2161 gcaaaatcga gttcagctcg tattttttcg atatcggaat aagcagtatc aggatttaat
     2221 ccgtaagagc gcatccgttc tttacggata ttgtttaaaa tatctgggtt attcgtcagt
     2281 cccacgattt ttttaggatc gatctcccaa agctgtttgg gaatatgtgc ttgtgggatc
     2341 aaaggtagat tggcaacttt taaatttttg ttagctaaaa acaagctcaa aggcgttttg
     2401 gaagttcggg atacgcctag caaaacgaca tctgcttcta gaaacccacg aggatctttc
     2461 ccgtcatcgt attttacagc aaattccatt gctttgatcc gtttgaaata gttctcattc
     2521 aataaatgaa gggcgccagg ttgccgaatg ggcttgatac cagttaagcg ctcgatttct
     2581 gatatcggcg gagtcatcac atcaaaacta aataattgat gttcttcaca aaaagaacgc
     2641 gtcatctcga tcaattcttg attgatcaat gtgtgcagga caagagcttt ttcttctagt
     2701 gcatcttgca gtgcctgcat caatgttttc tcatctgtca caaatgttct gcggaataaa
     2761 ttgaactcaa cgctaggata ttgggccata gt
//
LOCUS       DANKHJ010000209         2751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000209 DANKHJ010000000
VERSION     DANKHJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..2448
                     /locus_tag="PW113_003317"
     CDS             <1..2448
                     /locus_tag="PW113_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2527546.1"
                     /translation="GIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVT
                     GQLLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIV
                     DGLALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKD
                     ESKDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLL
                     DQPIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHL
                     TNLNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRK
                     EPLLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPK
                     EHEALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPL
                     ESGTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLL
                     MDQWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKI
                     GPNRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETAL
                     TGLIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILS
                     EKSVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQE
                     SVPKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAME
                     EIYQDIHEFLKKNEEGS"
     gene            2495..>2751
                     /locus_tag="PW113_003318"
     CDS             2495..>2751
                     /locus_tag="PW113_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2527547.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGT"
BASE COUNT          891 a          519 c          602 g          739 t
ORIGIN      
        1 ggcatcgata cagtagaact tgcacaaatt ttcttgccga cggaaccaag ctttcgctta
       61 gctgatttgt cagaaagcct tggatttatt catgagaatc ctcatcaggc agatagtgat
      121 gccgaagtga caggtcaact tcttttgctg atcgaagaac ggatgagaaa attgccaatc
      181 atcacgttag agcagattgc gcgattaagt agacacacag gtatggatac gagtcgtttc
      241 atttatcatg tgattgaaga aatgaaagaa aaaccagagc cacttgatcc ctcattagaa
      301 atcgtcgatg ggctagcttt gcagaaaaaa gaagtggaac tattcacatc cgtccattat
      361 ggcgagcgga catatccaag aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg
      421 atgtatcgaa aagaacagaa cagactgatg aatgcggtct acgaccattt cacaaaagat
      481 gaatcaaaag atttgatgat cgaagcagct acaggaatgg gtaaaacaat cggttatctg
      541 ctgccattga gttatttggc tacacctgaa aaacccgctg tcatcagtac cgtttcgcta
      601 gttttgcagc agcagatcat agaaaaagac attccattgc tgaatcaatt gctagatcag
      661 cccatacaac ctgtaattat caaaagctat cgccactata ttgatttgca gcgttttaaa
      721 ggcacacttg tggaaccgcc agaacaaaaa caatatgctt tgtatcaaat ggcggtctta
      781 gtttggctga cgcaaacgaa gactggtgat ttggatgaat tacatttaac aaatttgaac
      841 cattcattct ttgcagatat cgcgcacaga ggtattggat ttttagcaag aaatcagtca
      901 ttttacgaac aagactttgt tcgtttcttg caaaagaaaa tcaggcaaag taattttttg
      961 attgtcaatc atgcgttttt agtacaagaa acacagcgaa aagaaccatt gcttccagct
     1021 agtccatttt tgttgatcga tgaagtacat catttgccgg aaatcgctga aaaagtaaat
     1081 gacaaacgat taagtattaa ctatttccat aaacaaatcc agcaattaga agaaccaaat
     1141 cagctgtttg accgaataca aagctattta ccgaaagaac atgaagcctt acgctggtta
     1201 tcgatttatc gagaagaact ttctgcgcta tgcgaagcac agatttgtat ggcagaagga
     1261 ttaatgagtt tagtgagcgg caagcaacct aacggacagt atccagaaga agtcatgatg
     1321 acaaaagaga tgttggatca gctgccactg gaatctggga cagccattga tcacctgcag
     1381 cttttttatc aggagatcca gcagctacag tcacaaatgc gtctgacttt tgagattatc
     1441 caagaaagct ggacaaacca gatgcggatc gatttagcaa gtttatatag cctatttgat
     1501 caattagaca agcaatactt gctgatggat caatggctgg aagactggcg agatcatttg
     1561 atccattggt tcgttcctgg gaacacagcg cagcagatga tttttcatct ccatgatttt
     1621 caagcggcta tgttgccgtc gactgtttgg tatcctcgat atgatcgtat tctttatatt
     1681 ggaggaacgc tgaagatcgg ccctaatcga cactatctct ctaagaggct tggcattgaa
     1741 gcagcacctc ttaaagtgat tgcctctcct tatgattatg agaaacaggc aaggatctat
     1801 gtgccaaaag aggcacctgc cgtccatcaa gtcagcaatc aagcatatat cgattattta
     1861 gagacagcac ttacaggtct gatcgaacaa gaagatcggc caatacttgt gttattcact
     1921 tctcatgatg ttttgcagaa ggtctatcag aaaatgcatc tgtctatgct agaaaaagga
     1981 cgagaaatac tagctcaagg gtttggtgga agccgggaga agcttttgaa acgcttcatt
     2041 ttgtcagaaa aaagcgtatt gtttggcgct gacagctttt gggaaggtgt agatttgcct
     2101 ggagatgctt tgcagatcgt tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg
     2161 atggtcaaag ctaggaacgc ttatttagaa tcagagggga tcaatccttt ttaccaggaa
     2221 tccgtaccta aagcagcttt gaagttacgc caagcattag gtcgcttgat aaggacggag
     2281 acagatcgtg gcgtattgct tattttggat cgaagactag tcactgcaaa atatggcgca
     2341 cggatcatcc atgcattacc gaaaaaagta ccgatcaaag agctagctat ggaggaaatc
     2401 tatcaggata tccatgaatt cttaaagaaa aatgaagaag gaagttagag gagctgtctt
     2461 ttttcataat taaaatctgc tataatgaaa aggaatgaag ataaaggagc gacgaccctt
     2521 gaagaaaaaa cagacaaagg aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt
     2581 tttattagcg gtcatcgtga tcagctcgat tttttacttg cgttccagtc gtccggcggt
     2641 gcaggcgaag aaagaagcag tagaaatcgc aaagaaatat gcaaatcttg aggaaaccga
     2701 tcatttttat tggtttactc gaaagaagac atacttttca ctgacaggta c
//
LOCUS       DANKHJ010000210         2655 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000210 DANKHJ010000000
VERSION     DANKHJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            690..785
                     /locus_tag="PW113_003319"
     CDS             690..785
                     /locus_tag="PW113_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2527548.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1332)
                     /locus_tag="PW113_003320"
     CDS             complement(1087..1332)
                     /locus_tag="PW113_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527549.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="PW113_003321"
     CDS             1677..1961
                     /locus_tag="PW113_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527550.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="PW113_003322"
     CDS             1964..2380
                     /locus_tag="PW113_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527551.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          877 a          494 c          425 g          859 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt ttttt
//
LOCUS       DANKHJ010000211         2548 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000211 DANKHJ010000000
VERSION     DANKHJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            42..>2548
                     /locus_tag="PW113_003323"
     CDS             42..>2548
                     /locus_tag="PW113_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527552.1"
                     /translation="MVTSVFADVEKTSESTQSENQSFDKTTFSEESITSETTESQSEA
                     PTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGYSEAATTEELTQLLADESVA
                     KIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYINETPQSIEFQNFKGTAAHPG
                     GLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLYDGSKLGLIYAPRATVTLDG
                     VSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYGPYLGKFYGFLDSGDETGSG
                     KTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVTFKVSGSGSEFSVNTKINIT
                     NDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQGDSSQIHVSSGGQISVKMAG
                     DGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAVRFENGTQKLTVTNGGSLK
                     VRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTVTGEKSNIDIRADSGATVD
                     TTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVDLESPTYFDFQNKRIGSETN
                     GIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDPDYFSDMTDLSINGTDLGNF
                     VSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVPTNADKKIYGHVVVPEGVE
                     AKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDENEGTGYNPWGEGEQGGLFE
                     VQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTVDVIPPEPAKLSTTLINAAS
                     RSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIP"
BASE COUNT          814 a          467 c          612 g          655 t
ORIGIN      
        1 aaaagctgat tgcgtcgatt ttgtttgtct tccctctctt gatggtgaca tccgtttttg
       61 ccgatgtaga gaaaacaagc gaatcaacgc aatcagagaa tcaaagtttt gacaagacta
      121 ctttttcaga agaatcaatt acttctgaaa ctacagaatc ccaaagtgag gcgcctactt
      181 ctaatacaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
      241 ctgatagcag tgaagaggta caggttagag ttgtttcagc ggatgggtac agcgaagctg
      301 ccacaacaga agaactgacg cagcttctcg cagacgaatc agtagctaag attcgtttaa
      361 ctcagccttt gactttagac agggaattgg agatcaagcg agatatcgtt atcgattttg
      421 gcggacttgc tcataatttc ggcacacatc acatctatat caatgagaca cctcagtcga
      481 tcgagtttca gaattttaaa ggaacggcag ctcatccagg tgggttgata ccaaatatcg
      541 atggaaatgc cattatcatt gcttatatgt ccgatttttg gggaaacaga tatcatttta
      601 cgggagaaat aaaatttacc gggacacttg acttatatga tggttctaaa ttaggcctga
      661 tttacgcccc tcgtgcaact gtcacattag atggtgtttc cggtgtattg gatgtccaac
      721 cagtgaaaga agggatgaat gccgctccgg gtaaagctta tttttgtaga agctatcagc
      781 taaatgtaat caatggtagt cagctttatg gaccatatct aggaaaattc tatggttttc
      841 tagattcagg tgatgagacg ggcagtggaa aaacagcacc aggactccat atcttgagtg
      901 gttcaaaggt ctctcttgac tatgatcggg cagatggcgt cagcgatgaa ggagaggctg
      961 tcgatacgct acccgggaat gtcacattta aagtcagtgg ctcaggaagt gaattttcgg
     1021 taaatacaaa gatcaatatt accaacgaca acaatcgcgg aattatccaa atgagaggct
     1081 caggatcaag agtcgctgtg tcgaatgatg ggaaaatcac aatcaataca gcgactactg
     1141 gcggatttcg cctgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
     1201 cggtcaaaat ggctggagat ggcgaccaac ctggaaacaa tgggatgcgt tttgtaggga
     1261 aaggactttc tttgatcgta gaaggagccg gatcagtcat cgatattgat aagcaaagtg
     1321 gtcgttctag cgcagttcgt tttgagaacg gtacgcaaaa actaacagta actaatggag
     1381 gctcattgaa ggtaagaaat gcaggggatg gcaaatctta tgtgaacaga ctcaatcaag
     1441 gaaaccaagc gatccaattt tatgacgctt ctggttttct agaaagtcca gggtcagctg
     1501 attttacggt tactggagaa aaatcaaata tcgatatacg cgcagattcc ggagctaccg
     1561 tagatacaac tggagatatt gatttgaact ttatggctgg cgaaaaaact tatttagtca
     1621 tggtcgggaa aactggggaa gataagactg gcatcatcag tggggatgtg ttgaatgtag
     1681 acttagaatc tcctacctat ttcgatttcc aaaacaaacg gatcggaagc gagactaatg
     1741 gcataggtgg ctgggtattt caaggaaatg gcgattcttc tttgaagatc gctcattcag
     1801 aaattgcttt atggcgaaag gcaggagtaa cgttcgataa ctttgaaaaa gatccggatt
     1861 atttttctga tatgacagac ttatcgatca atggaaccga cttaggtaat tttgtatcaa
     1921 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
     1981 atcgaatcag tgccaataac cagcttccga tgattgaatc cttaagagta ccaacgaatg
     2041 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
     2101 atgcttggac aggcgaagta gaagtgactt tacgtattac ttatgcagat acgacgaaat
     2161 cacctgtaga aattactgct ctcacacaag gacaaacaga tgaaaatgaa gggacaggat
     2221 ataatccgtg gggagaaggc gaacaaggcg gtttgtttga ggttcaagta cccaacgaag
     2281 aaagactaac aacgggggat caagtgcaaa tagttgctgc taaaaaagta agaggcggcc
     2341 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgagcctg
     2401 ctaaattaag taccacattg atcaatgcag cttctcgctc aatcacaggt acaggcgaag
     2461 caggagcttt ggtttcattg aaaaaaaatc aagctgtttt gtccgaaaca actattgatg
     2521 atacaggtaa atttgttctt tctatccc
//
LOCUS       DANKHJ010000212         2541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000212 DANKHJ010000000
VERSION     DANKHJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..405
                     /locus_tag="PW113_003324"
     CDS             <1..405
                     /locus_tag="PW113_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2527553.1"
                     /translation="PFMGNGDVKTPEDAKWMLDYVGADGVMIGRAALGNPWMIHRTQH
                     YLETGELIPEPTPAEKIATAKLHLQRLADLKGEKVACREFRTHAAYYLKGIPRAAKVK
                     VAINQTEKQQEINDLLDQFVEKAEKVLLKQTL"
     gene            516..2012
                     /gene="lysS"
                     /locus_tag="PW113_003325"
     CDS             516..2012
                     /gene="lysS"
                     /locus_tag="PW113_003325"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2527554.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT          941 a          437 c          533 g          630 t
ORIGIN      
        1 ccatttatgg ggaacgggga tgtcaaaacg ccagaagatg caaaatggat gcttgactat
       61 gtcggtgcag acggtgtgat gattggtcgt gcagcgctgg gcaatccatg gatgattcac
      121 cgcacacagc attatctcga aacaggggaa ctgattcctg aaccaacacc agccgaaaaa
      181 attgctacag ctaaacttca tttacaacgc ttagcagatc tcaaaggaga aaaagtagct
      241 tgtagagaat tcagaacaca tgcggcttat tatttaaaag ggattcctcg agcagcgaaa
      301 gtgaaagtag caatcaatca gacagaaaaa cagcaagaaa tcaatgacct gctggatcaa
      361 tttgtagaaa aagcggaaaa agtactattg aaacaaacgt tgtgatttct aagtttttcg
      421 tgaattttca agccgaaatt ccaagttcgg cttgcttttt ttgtgtttca ttggcattat
      481 aggaaagaga ttaacatgag aatggaggaa cttttgtgac acaggaacaa caacatcaag
      541 tagaagattt aaatgatcaa atgcttgtac gtcgtcaaaa aatggaacaa ttgcgtgaag
      601 atggaatcga tccatttgga aaacgatttg aacgcacgca caactcagaa gaattgcatg
      661 aactatttga tccaagaaca aaagaagaac tagcagaaat gggattgacc gctagtgtag
      721 ctggtcgtat gatgactcgc cgaggaaaag ggaaagctgg atttgcgcat ctacaagatc
      781 gtgaaggcca aattcaaatt tatgtaagaa aagaccaagt aggtgatgag gcctatgaag
      841 tattcaaaca tgcagacctt ggagatttct ttggtgtaac tggacaaatc atgaaaacag
      901 acacaggcga agtttctatc aaagcttcag aaatcacgat tttgtcaaaa gcacttcgtc
      961 cactgccaga taaataccat ggcttaacga atattgaaca acgctacaga caacgctact
     1021 tggatttaat cagtaatcgc gaaagctttg accgatttat gaaacgaagc caaattatca
     1081 gcgaaatccg tcgctattta gatggcaatg gctatattga agtagaaaca ccagtattgc
     1141 acaatgaggc aggtggagct gccgcacgcc catttatcac gcatcataat gcactggaca
     1201 ttgatttata tttgcgtatt gcgctagaac ttcatttaaa acgactaatt gttggcggaa
     1261 tggaaaaagt ttatgaaatc ggccgcgttt tccgtaatga aggaattgat actacacata
     1321 atccagaatt tacgatgtta gaagcataca cagcatatac tgatttcaat gatgtaatga
     1381 atctgacaga aggtattatc cgtaacgcag ctgaaaaagt actaggaaca gcaaaaatca
     1441 catatgatgg acaagctgtc gacttagaaa gtgactttaa acgcatccat atggtagatg
     1501 caatcaaaga acaaactggt gtagacttct ggcaagaaat gacattggaa gaagcattag
     1561 ctttagccga aaaacacaat gtagaagtaa ccgaagctat gggtgtcgga catgttatca
     1621 atgaattctt tgaaacattt gttgaagaaa cattgacaca accgacgttt gtatacggac
     1681 atccagtcga agtttcgcct ttagcgaaga aaaatccaga agatcctcga ttcacagatc
     1741 gattcgaact attcatagtg gggcgcgaat ttgctaatgc ctttacagaa ctgaacgatc
     1801 caattgatca aagagaaaga ttcgaagcgc aagaaaaaga acgtgaacta ggaaatgacg
     1861 aagctcacgg tgtagacgaa gacttcttgg aagccttaga atacggtatg ccgccaactg
     1921 gtggtctagg catcgggatc gacagattag taatgttgct aactgatgca caatcaattc
     1981 gggatgtttt gctattccca acaatgagat aatcgaataa aagctgtcta aggaattcct
     2041 taggcagctt ttgctgtgta gaaaggaata aataataacg aaagttcaaa agctaatgcc
     2101 gactattcta gacattaaag aagacgaaaa cgaacgataa agaaagaaaa gttatgaata
     2161 atcgtttatt gaaaaaaact tgaaaaaata caaaaaattc gttgaccaag ctaaatttag
     2221 ttggtatatt attacttgtc caaaagacag aagaaaatat ttattcaaaa acataacaaa
     2281 aaagttattg acatgttact aacgacatga taagataact gagtcgttaa aaaagaacca
     2341 cttgcgaagc ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaacg
     2401 ataagttatg ataaatgagt tgccgaaaga tttcgaaaaa cgaaatcgaa aaaaactttt
     2461 aaaaaagagt tgacaatcgc ttgtctctct gatatgatat aaaagttgct acggcagcca
     2521 aaaatgaatc gggaaacact c
//
LOCUS       DANKHJ010000213         2539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000213 DANKHJ010000000
VERSION     DANKHJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..390
                     /locus_tag="PW113_003326"
     CDS             <1..390
                     /locus_tag="PW113_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2527555.1"
                     /translation="FQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFE
                     QEYIFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDEL
                     TVSYNRARQGAITQEITEIVAGASALE"
     gene            413..1819
                     /gene="atpD"
                     /locus_tag="PW113_003327"
     CDS             413..1819
                     /gene="atpD"
                     /locus_tag="PW113_003327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2527556.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            1833..2255
                     /locus_tag="PW113_003328"
     CDS             1833..2255
                     /locus_tag="PW113_003328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2527557.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT          816 a          475 c          572 g          676 t
ORIGIN      
        1 ttccaaaacg aagtattcga tgaattatac gtatgttata accatcatat caattcgcta
       61 actagtcagt ttcgtgtaga aaaaatgctg ccaatctcag acttagatcc agaagaagca
      121 acgactttcg aacaagaata tatttttgaa ccttcaaaag aagaaatctt gtctcagtta
      181 ttgcctcagt atgctgaaag tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa
      241 catgctgctg gtatgacagc aatgaaaaca gcaactgata atgctgctaa tatcattgat
      301 gagctgacgg tttcttataa ccgtgcaaga caaggggcta ttacccaaga aattaccgaa
      361 attgttgccg gagcttcggc actagaatag tattgagatt ggaggaaaaa agatgagttc
      421 aggcaagatt gttcaagtaa tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc
      481 cttaccagat atcaacaacg cattagttgt ttataaaaat gacgaaaata aatcaaaagt
      541 tgttcttgaa gcagcgttag aattagggga cggagtgatc cggaccattg cgatggaatc
      601 aacagatggt ttacaaagag gaatggaagt cattgataca ggcaaagcaa tctctgttcc
      661 tgtaggtaaa gaaacattag gtcgcgtatt caacgtacta ggagatacga tcgatttaga
      721 aacacctttc ccagaagatg cggaaagaag cgaaattcat aaaaaagcac cagcctttga
      781 cgaattaagt acaagtacag aaattttgga aacagggatc aaagttatcg atttgcttgc
      841 cccatattta aaaggtggga aagtcggact attcggtggt gccggtgttg gtaaaaccgt
      901 actgatccaa gaactgatcc ataatatcgc ccaagaacat ggtggtattt ctgtatttac
      961 cggtgtaggt gaacgtactc gtgaaggtaa tgacttgtat tatgaaatga aagattcagg
     1021 agttatcgaa aaaacagcca tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat
     1081 gcgtgttgct ttgactggtt tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga
     1141 tgtactgttg tttatcgaca acattttccg tttcactcaa gctggatctg aagtatcagc
     1201 cttgttagga cggatgcctt ctgcggttgg ttatcaacca acattggcaa cagaaatggg
     1261 tcaattacaa gaacgtatca catctacgaa aaaaggttcg atcacatcga tccaagcaat
     1321 ctatgtgcca gccgatgact acactgaccc ggcgccggca acagcattcg cccatttgga
     1381 tgcgacaacc aacttggaac gtaaattaac agaacaaggg atttatccag cggtagatcc
     1441 attggcttct tcttcaagtg ctttagcacc tgaaatcgtg ggggaagaac attataaagt
     1501 agcaacagaa gtccaacacg tattgcaacg atatcgtgaa ttacaagata tcattgctat
     1561 cttaggtatg gatgaattat ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt
     1621 ccaattcttc ttgtcacaaa actttaacgt ggctgaacaa tttactggtc ttccaggttc
     1681 atatgtccct gtagaagaaa cagtgaaagg attccgcgaa atcttggaag gcaaatacga
     1741 tgatcttcct gaagaagctt tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc
     1801 gaaaacacta ggctactaga aaggggtgcc ccatggatca atatttaaca gtaaatgtgg
     1861 taactcctgc cggtttggtc tatgatcacc atgcagctat cgtcgttgca cgtacgacag
     1921 ctggtgagct tgggattttg cctaaacatg caccaatcat cgttccattg acgattgatg
     1981 aagttcgtgt aaaacgaaca gattcagata cccacgtcga ttggatcgct gttaatggcg
     2041 gaatcatgga agttcgtgat aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac
     2101 gtgatatcga tgtatctcgt gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag
     2161 aagcaaaagc aaaacaagat acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag
     2221 caatcaatcg tatcaaagta tctaagcact cataaaatga gatgcggatg tttatccgtg
     2281 tttaagaaca gtaaaaagag gttatgacag aagtggtcag cttcaagaca taagaaggaa
     2341 tttacgaaaa ttgcttctca aatttttgtg aaatctcagc ttatttttcg aagaagctac
     2401 ttctggaaca ccgtatatca gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa
     2461 acaaatggaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagat
     2521 gattgaacac cgtttattt
//
LOCUS       DANKHJ010000214         2512 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000214 DANKHJ010000000
VERSION     DANKHJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..494)
                     /locus_tag="PW113_003329"
     CDS             complement(<1..494)
                     /locus_tag="PW113_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527558.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(487..801)
                     /locus_tag="PW113_003330"
     CDS             complement(487..801)
                     /locus_tag="PW113_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527559.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1017..2408)
                     /locus_tag="PW113_003331"
     CDS             complement(1017..2408)
                     /locus_tag="PW113_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2527560.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
BASE COUNT          710 a          465 c          360 g          977 t
ORIGIN      
        1 tataaagtaa atggcaaccc aactatgtaa ccattctctc tacgtactac gatttggtag
       61 tcctgccttt caatcggtaa ttcataatag taatttactg tttcttcttt gccactttca
      121 atatactctg gattatccaa tatcatatca tactcataaa gttcttcacc atccttattt
      181 tcaatcgacc attgttgtgg acgtatcgga ttttctgtat catttataag tgtataggtc
      241 acttgtaata cacgatcatg ttgtgcaaat tcatttcttt tttcggtcac ttcaatattt
      301 ttaaatgtta gttcaacccc atctgcctga taagtttcac ctatttttaa atcttttgtt
      361 acaattttta ttggcttttc ttctggaaaa tgtctggctt taattgttag ataacttcca
      421 aaaagaatca atcctagtag cactaaaaga attcctttaa cgagccactg aaacgggaag
      481 taaactttaa acatcttttt tctcctccgt tttgtatttg ttttttagag aatacgataa
      541 cttttgatca atgtcatcca gcttttcaat tacttcatta cttatcattg attctagttc
      601 tgaaattttt tgttcaaatt taatgctttc aggatcatta aatttattat ttgatggact
      661 agctaacact ggtaaatttg aaaccataac acgaaacata tttgcctttg aaccaaaaat
      721 tcctttgttt tggttgtaaa aattgttgaa tatttcttta gtttcttctg aagccgagac
      781 tttaaaatat ggatagttca tttttttctt ccacctttct ttttttggga gggagtctgt
      841 agggagcttg ctccctcaga gatttacccg ccatctgact tgccagaaag tagcctagcg
      901 tgagcggggc ggattgactg gcaagtagat gggtagctgg cgtatgtcct aaaaactgct
      961 tctttccaag caactcttca taggcaaaaa actggaaagt ccactaacaa gcccattcag
     1021 aattccattt caaaattttg ttgccttttt tgctgatttt caatcatttg ttgtgtgaat
     1081 tcataatcct ttttagcacg atacattttt acatcgtcat tcattatgcg actgatttga
     1141 tagtgaatct gtttagcaga aggtgtatga gaacaatttt caaaaccctt tgccggtaga
     1201 aataactctt tctgatcagt ctttttaata gaaattgttt tacttttcgc actacgtaaa
     1261 gtttgaggat tcacatccgt atgaagttta atcgtgtatt cttgatcatc ttctaaagac
     1321 attgtattta caaaatgctt gtctacctct cgcaagaagt catcatattt aatgaatttt
     1381 tcatcgcctt cacggtcgct aaattgcttt tgtgcgatgg aatctaattc cacaaacggt
     1441 acatttaact caccagtttt gtaatttact ccactaatta gcgacataac ttgctctctt
     1501 tccatattaa aaaaattttt tatatgtgat tcttctaaat tcttttcaag ttgttcagaa
     1561 acagattcaa atgaatatct tttccctaac tttcttgcac taaaagtacg ttcgacatac
     1621 tgactttttt cttttctaga ttgtttatct cttcctaaag taattaactc acctctacgc
     1681 tcatagaaat ttacaccttg tttgtaaaga acatagccaa gtttgttatt tgtttttgaa
     1741 aattgataaa cttcaatgtt atattctttt gccaaatgat ccgaaaaatc ttcaaaagaa
     1801 ttcacttcgc atttttccaa cgttgcttcg atcgcatttt gcacaaactc ttgataatta
     1861 atttttccat atttcatctt agaaggatcg tttgaataaa gattggttct cttttctaag
     1921 ttttcatagt actcattatt cactgattca agaccatgtt cattgaggac ttgatcgtta
     1981 atttttcgca ttagttctaa atctttaggg tcaatacgca accttttaca tgtttccatg
     2041 ttaatattat taatgacaag atgactatga aaatgatctt tatcattgtg acttgcaaca
     2101 atgatttgcg aatttggaaa agctttctct gcaaattcga cagctatttc gtgcatttta
     2161 tcaatactta actctttaga agtactctta tcaaacgaat gaataacatg aaacgcttgt
     2221 gctatatctt tagatttatt catcttggta cgtactaaat tcatttcttg cagtgcttcc
     2281 tcactatcat cactacaatt tattcctgag actgcaacac atttttcttt ttttgaagga
     2341 ttagctgcat attgtaatag cgaggataaa tttttcttgc gtcctttcga tgttatagta
     2401 gctcccaaag ttgtttcacc tcatctttta aacttgcaat aatctgaatt gagtgaatta
     2461 aattccgatc aactttttgg gaatactctt ctttcgtacc cggtctagtt tg
//
LOCUS       DANKHJ010000215         2496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000215 DANKHJ010000000
VERSION     DANKHJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1251
                     /locus_tag="PW113_003332"
     CDS             <1..1251
                     /locus_tag="PW113_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2527561.1"
                     /translation="KSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            1271..>2496
                     /locus_tag="PW113_003333"
     CDS             1271..>2496
                     /locus_tag="PW113_003333"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HDL2527562.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKAT"
BASE COUNT          799 a          480 c          591 g          626 t
ORIGIN      
        1 aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga acctgtatat
       61 ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac agacggctac
      121 cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg aacacatgaa
      181 tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg
      241 atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt gacagctgtt
      301 atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa attgggcaat
      361 tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa attcttgctg
      421 gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc cagagctcgt
      481 ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga gattagtaat
      541 ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga attgttcatc
      601 gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc
      661 atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga caagatcttg
      721 gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg agaagatttt
      781 gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga tgtcgacggc
      841 gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc tattgttgaa
      901 gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg gaagaatatt
      961 acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt tgcttcactt
     1021 cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat ggatgatcat
     1081 caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt cagtgtggac
     1141 gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt agaaccccgt
     1201 cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta atcatgaagg
     1261 aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc taaccagcga
     1321 aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg cacttcctga
     1381 tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga acgaattagg
     1441 tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa
     1501 ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg cacagccatt
     1561 cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg acggtgacgg
     1621 tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg
     1681 tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag aaaaagaacc
     1741 agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt
     1801 aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg ctatcgattt
     1861 gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac ctggaccaga
     1921 ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt atgaaacagg
     1981 taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa atggtaaaga
     2041 acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga tcgaacggat
     2101 ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg acgaatcttc
     2161 ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat ccgttatctt
     2221 gaataactta tataaattga catccttgca aaattcattt ggattcaata tgttggcgat
     2281 cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact atatcgaaca
     2341 tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag aagcaagagc
     2401 acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca ttgcgattat
     2461 ccgcgggtct aaatcagatg acgaggcaaa agcaac
//
LOCUS       DANKHJ010000216         2480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000216 DANKHJ010000000
VERSION     DANKHJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            145..648
                     /locus_tag="PW113_003334"
     CDS             145..648
                     /locus_tag="PW113_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2527563.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            775..1812
                     /locus_tag="PW113_003335"
     CDS             775..1812
                     /locus_tag="PW113_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527564.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1827..>2480
                     /locus_tag="PW113_003336"
     CDS             1827..>2480
                     /locus_tag="PW113_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDL2527565.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     "
BASE COUNT          860 a          305 c          529 g          786 t
ORIGIN      
        1 ggatttaaac gggagttttc caaactgatt ggcttcttgg tgattgcttt gattgcggta
       61 ggtctggtct ttaatgcttc tggtgtgaag gatgtcctac tcaatttgtt taaccgaatt
      121 gtcggtgcct gagggggaat cacaatggaa cagatgcgtg tttatattgc gaaccttgga
      181 aaatacaatg aaggtgagtt ggtgggggca tggtttaccc cacctgtcga ttttgatgaa
      241 gtgaaagaac agattggatt aaatgacgaa tatgaagagt acgcgattca tgattatgaa
      301 ctgccttttg agattgatga gtatacgcca attgaagaga ttaatcggtt gtgtaactta
      361 gcggaggaac tagaaggaac gccaattggt gaagctgctt ctgagattca acacgctttc
      421 ttcaattctt tcgaggaaat ggtagagcat gtggatgata ttgtctatta tccagattgc
      481 gatgatatga gtgatgtggc gcattacttg attgtagaag ctggtgattt tggcgaagtt
      541 ccagaacaat tgaagccgta tattgatttt gatgcttgcg gtcgagattt ggagattgga
      601 ggaaattatt tggttactag tcgtggagtt tttgagtatc cagaatgaat caaacactat
      661 atatagtgtt tgattagtat atgtaagcaa aaaaacacaa tatatagagt gcaaactgaa
      721 gggtaatgtg atataatatt tgccgaagaa atatttttga aatgaggtaa tattatggca
      781 aatattcaaa caagttttat tgatttccat aattcaatta gattggacgt agaagacaat
      841 acgctactta aggactataa agaccaagta attgatggat taaaagatta cttacctgat
      901 gatgtgaagt ttgaaacttt tttacaggga agctattctg tttataccgg aataaaaagc
      961 tgtgatgaaa aaattgattt tgacatcgat attgcagttg ctttcgaaat tgatcatact
     1021 gtttatgaag atccaaggga gcctaaactg tgggtaaaag aggcgctagt tgaaattttt
     1081 cctaatgctc aagttaattt aaaagtacct tgtgtaactg ctacatttac tggaaaaaag
     1141 acaaaaaaaa atgtgcacgt tgatgttgcg gtttatgcta aagaagatga aaattatttc
     1201 ttagcaaaag caaaagaatt cagtgctccc gaaaaccgat gctgggagga agcggatccc
     1261 aaagtattaa aagagaaaat taatagtcat gtagcagatt cagatgatcg aaaacaattt
     1321 cgaagatgta ttcgctattt aaaaagatgg aaagataaca attttaacca agaatataaa
     1381 ccaacaggaa ttggacttac aattaatgtg atggatacgt tcctagttaa taagtccact
     1441 gattttttaa ctagaaaagt tcagtataat gatatggaat gtatgaagca gattgtttct
     1501 tcgctgaaag attcatttgt gtatgagtac agtgaaacag atggttggca ttatcgtcta
     1561 catgcatatc ttccagtgaa gcctaacagt gacacctatt caaaaatgac agtgaatcag
     1621 atgagtgatt ttaaaaacaa actttctaaa ttatatgatg atttgatttt cgctatcgat
     1681 actgaagatg agtatgaagc tacaaaacga ttaaataatc aatttgggga ggatttttta
     1741 attatttcag aagaagaagt tacagaaaaa aacttaagaa atgcatttgt tacagactat
     1801 ccgagtgctt aggttggtgt taaagattgg attatactga tattttaaaa gaaaagctaa
     1861 cagatgttgt gcgtttattc gatggtaatt actatgggga tttttcattt gctacaattg
     1921 gaaagttgcg tttaaaaatt gaaataccaa aacattttcc attggtactt cctaggattt
     1981 acattcataa ttttgatcat ttaggacttt acctaccaca tgtagaaaaa gatgggttgg
     2041 tatgtttttc ttcagatata aattatttag tagatgaaga aaattcagaa ttggttttat
     2101 tgcagtgtgt taataaaact ataaaaacaa ttgaggaagg tttgaaaggg ttaaataaaa
     2161 atgattttcg aaaagagttt gttgcttttt gggacaaaca agagaatcaa atttcgttat
     2221 tctacctttg tgaaccagcg ttgtttatca agtcagttga tacaataatg tacggtaagt
     2281 ctttggttgc tttggataaa gagaacgaat tacaattaaa tgcagcgagt agattgttta
     2341 atggagagaa agaagatact catgaattag atgcatacta tgttccaatt agaaacaaga
     2401 ataatattat gcccccgaag aataataaag catggtcaaa gaaggatatt ttacaaatag
     2461 taaaaaaaaa tgtaacaggt
//
LOCUS       DANKHJ010000217         2438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000217 DANKHJ010000000
VERSION     DANKHJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            316..570
                     /locus_tag="PW113_003337"
     CDS             316..570
                     /locus_tag="PW113_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2527566.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="PW113_003338"
     CDS             571..888
                     /locus_tag="PW113_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2527567.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="PW113_003339"
     CDS             1447..2382
                     /locus_tag="PW113_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527568.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          884 a          345 c          458 g          751 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DANKHJ010000218         2422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000218 DANKHJ010000000
VERSION     DANKHJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            8..697
                     /locus_tag="PW113_003340"
     CDS             8..697
                     /locus_tag="PW113_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL2527569.1"
                     /translation="MKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAENWADLFW
                     EDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKELKDQLP
                     SIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMVRNKIRV
                     ICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMPSTATMT
                     IIKQMELLEDE"
     gene            876..1217
                     /locus_tag="PW113_003341"
     CDS             876..1217
                     /locus_tag="PW113_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527570.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1210..1956
                     /locus_tag="PW113_003342"
     CDS             1210..1956
                     /locus_tag="PW113_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2527571.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            1962..>2422
                     /locus_tag="PW113_003343"
     CDS             1962..>2422
                     /locus_tag="PW113_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527572.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRV"
BASE COUNT          892 a          389 c          509 g          632 t
ORIGIN      
        1 agtaattttg aaaaataaag aagctgtgac tacgagctta caagttgcag aaagcttcga
       61 taaaaagcac agacatgttt tgacggctat tgatgaacta aaagaggggg ttgccgaaaa
      121 ttgggcagac ctattttggg aagatactta tgttcatcct caaaataaac aatcataccg
      181 aattatttat atgaatagag acggcttctc tctattagca atgggcttca ctggtaagaa
      241 agctttaagt tttaaacttc aatatattga ggccttcaac aagatggaaa aagaattaaa
      301 agaccaattg ccatcaattc cagcaactaa acgagaaatg gcattacttg ctctagctgc
      361 aaacgaagaa acgaatgaaa gagtagacgc tatcgagtct gatttaaacg atttgaagaa
      421 caatcaactt ctagctgaac cagattaccg aacaatttct aatatggttc gcaacaaaat
      481 tagagttatt tgtaatcagc agcatttaaa tagcaaagca aaagcagaat tgtttaaaga
      541 cttgaatggc ggaattaagc gaatcactgg agcagtagct agaaatcgta tcaaggcaaa
      601 acagttcgat gatgttatcg attttattaa caattggatg ccttctacag cgacgatgac
      661 aattattaag caaatggagt tgttagagga tgaatagagc tgaagcgcta aaaataggcg
      721 taataattgc taatcgctgg tggagacaca ataaaccaaa catcctaagc caacaacata
      781 ttgataagca aaaagcatgg caacaaataa aaaagtgact cagccgacca aagcaatgag
      841 tcacaaagaa aatacatcta aggagatgtt accacatgaa aaaagaactt tccactctag
      901 atcaatattt gactgatcct agttggggca aatcgaatat caaggaaaca agcaatcgaa
      961 aaatcagacg taatcttttg acggatgaag aactagcatg tgatcaagac gatttgggaa
     1021 attttgtgag tatttgggat catgtctatc ttatccattt atcgaggaag tccaaaaaaa
     1081 ctgaatatat ctatgtcatc gaagatggct tgattgatgc gctagaagag tatgacagag
     1141 ataacttgat tgatatctct tattacggat caggtaagaa atacattgct gaaatggagg
     1201 cagaatttga tgagtgaaaa ggaacaacct ttgaaaaata gaagtgataa tacgcttttt
     1261 aataccttat acaaaataaa tgtgaaagat gttactgaaa aacgaaataa cctcacttat
     1321 ctttcgtggg catgggcttg ggcagaagtt agcaaagtgt gtgaggcagt agattatgaa
     1381 atctatcatg atccagaaac atatctgccg tatgtctttg ataagaaaac aggctatatg
     1441 gtttttacca gtatcacagt caacggagta aagcgtgaca tgtggttacc agtcatggat
     1501 ggtgcaaaca aggcaatgaa agatgagcca tatacctacg aagtcaatga ttatcagtgg
     1561 aataacgaaa cgaagaaaaa agagattgtt ggaaaaatcg aaaagcgagt tgaagcagca
     1621 actatgtttg atatcaacaa aacgatcatg cgctgtcttg taaaaaatct agcaatgttt
     1681 gggctagggc tatatatatt tgctggcgaa gatatgccag aagatgtctc gatgcttgaa
     1741 ccagctactc aaagaagcaa aaagctattc ttggatgctt tacaactggt tgctaacaag
     1801 tacgaaaaat caattgatga agcaattgtt gcattgactg atgcggcttc tataaccgct
     1861 gatgacagta aatggaccaa gagagacttg ggcattctaa aacgaggcgt taactggatt
     1921 gaagatcagt acagagaaga aacaaaagag aagtgatatg agtgtttaaa ccattgatcg
     1981 attcatattc agcggttctg aaaaagttca aaggaaaaga cataggtgca acgatcaatg
     2041 aagaagtgaa cattgatcga ctaaagacga tgtatgacgg atatgatggc gatcggatta
     2101 ttgaagttcg atttattgat ccacgtcgat ttactgtgca gcaacgaaac ttcatctatg
     2161 cgctgatagg cgatattttc atcgatacag gcatgccaac ggacttctgg aaggaattct
     2221 tttacttccg ttttgaaggc gtcacagggc gcaaaataag cctcaaagac gaatcgaata
     2281 caactgtgag tgatgctaat gtcttagcaa atatcatctt agatttcatc tttgaacatc
     2341 atattccttt caaagaaggc tatgagattt taccagcgaa tcaagaatat tacttctaca
     2401 aatgcatcac aaaaagagtc tg
//
LOCUS       DANKHJ010000219         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000219 DANKHJ010000000
VERSION     DANKHJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            207..2069
                     /locus_tag="PW113_003344"
     CDS             207..2069
                     /locus_tag="PW113_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2527573.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="PW113_003345"
     CDS             2069..>2400
                     /locus_tag="PW113_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2527574.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DANKHJ010000220         2370 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000220 DANKHJ010000000
VERSION     DANKHJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..335
                     /locus_tag="PW113_003346"
     CDS             <1..335
                     /locus_tag="PW113_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527575.1"
                     /translation="VDDVIIKEKVTSADVARVRENMSFFKDSPWKTVVMQYLGFADIQ
                     IAQVTQLDQLFDTMLKDGQVTATATYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQ
                     MGYSNISY"
     gene            381..1325
                     /locus_tag="PW113_003347"
     CDS             381..1325
                     /locus_tag="PW113_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2527576.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1341..2120
                     /locus_tag="PW113_003348"
     CDS             1341..2120
                     /locus_tag="PW113_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2527577.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2132..>2370
                     /locus_tag="PW113_003349"
     CDS             2132..>2370
                     /locus_tag="PW113_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2527578.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRV"
BASE COUNT          841 a          442 c          510 g          577 t
ORIGIN      
        1 ctgtagacga tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg
       61 aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat
      121 tcgctgatat tcagatcgcg caagtcacgc aactagatca gttattcgat acgatgttaa
      181 aggatggtca agtgactgcc accgctactt acgatcaata tttgacggtg ctcagtcaaa
      241 tagagcagat ccggaatgaa aaaataagcg cagcatacgc aacaaaagca caaactgttg
      301 ctgagcaaat gggctatagc aatataagct attgacagtt aaaacgttga ttcaagcaag
      361 gttagcaaac ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat
      421 ttctgattat tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa
      481 aattatatct ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc
      541 aaggagaacg aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga
      601 tggggattga tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg
      661 ttgccacagt cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgtgatacgt
      721 atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg
      781 gagctgccat ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg
      841 tctctatcaa tatgaaaggg ctaaaggaac tcgttgatgc agttggtggt atcgaagtca
      901 ataatgacct cactttctca caagatggct atgactttcc aatcggtaaa attacattga
      961 acggggatca ggcattatct tattctcgta tgcgttatga agacccaaat ggtgattacg
     1021 gtagacaagg gcgccaaaga aagatcgtag aaggcgtagc gaaaaaagta ttgagtctaa
     1081 acggtgtcag caactatcaa tccgttttga atgctctaga gtcgaatatg aaaacagaca
     1141 tgaactttga tgaaatgaaa aaaattgctt tagattatcg ggaggcattt ggtacgatta
     1201 agcaagatca gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca
     1261 gtgatcaaga acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata
     1321 aataggaaag tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttgaaacat
     1381 tacgcaaaca cttggtaata atcatcatct caatgtttgc tggcttggcc atctcaggaa
     1441 tcgtaacatt tttcatcttg acaccaaaat acagttcaca agcacaattg atcgtgacac
     1501 tgccacaatc agacactgca aatgtcaatg atgtcaatac gaacctgcaa atgatcaata
     1561 cgtataaaga tatgatcgtc agtgatttag tgctcaatga agtcaaagat cgtttagaaa
     1621 cagaagacaa tgtgaaaatg actgctgaac aaatcaaaga tgccatctca gttaatcagg
     1681 cagaaaattc acaaatgttc tcgattcagg caataagtac gaatgcagtg gctgctcaac
     1741 agatcgctaa cacaacttca caagttttcc aagagaacgc aaaagatgtg atgaatgttg
     1801 ataaaatctc gatcatttca ggtgcagttg ccaatacgac acctgtttcg ccaaataata
     1861 aattgaattt aatgactggc ttaatgttag gaatgatggt aggtgtaggt ctggcattct
     1921 tattggaatt actcgatcgg acagtgaaag atgaaaaata tgtgacagat acattaggat
     1981 tcccaatctt aggaacagtt ccagaaatgt cagcgaaaga attgaatgct ggtatccgta
     2041 aaaaaggacc agctcagaca caaccaaaag ctacaaacga taaaacagcg ccatcacgca
     2101 gaagccgctc aagagtctag gaggaaagaa aatggcacga acacagaaac aaaaagcagc
     2161 ccaaactcat gcagtcagtt tggtcacact caccaatccc tcttcaccaa tcgctgaaca
     2221 atatcgtacg attcggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac
     2281 gattgttgtg acttcatcag gacctggaga agggaaatcg accactgcag caaacattgc
     2341 cgttgttttt gccaaatctg gtcaacgggt
//
LOCUS       DANKHJ010000221         2336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000221 DANKHJ010000000
VERSION     DANKHJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1151)
                     /locus_tag="PW113_003350"
     CDS             complement(<1..1151)
                     /locus_tag="PW113_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2527579.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGV"
     gene            1406..1972
                     /locus_tag="PW113_003351"
     CDS             1406..1972
                     /locus_tag="PW113_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2527580.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            2117..2204
                     /locus_tag="PW113_003352"
     tRNA            2117..2204
                     /locus_tag="PW113_003352"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Ser,seq:cga)
BASE COUNT          892 a          410 c          426 g          608 t
ORIGIN      
        1 acaccagcta ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc aagacctgtc
       61 ccaaacacag ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa
      121 gaagcaataa atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca
      181 ctgaaaccca ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc
      241 agcaaaacat aagaagacag catcagacca actaagccca tgttgatcaa agtagcacca
      301 ataccagaat tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga
      361 atccccgaaa gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag
      421 ccaaaaataa tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg
      481 attgaatagc tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg
      541 aatccaatat tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc
      601 ggaagaagta taccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa
      661 ggaaaacgca taccaaaagt gatataacta acgaccggtg ataatacgct accaaacagt
      721 ccgatcatga tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct
      781 aaaatgattg agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt
      841 agaatcgaag caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca
      901 gaaagtaagg tcaaaatccc tacatttatg aatgcgcttc cgaaaacacc taaagcaaaa
      961 taatctgtca aaagattgct tggcgaggta agaatagtta gcaatccact ccaattatca
     1021 gtaggagtat tagaaagcaa gccaataaaa agaaaaaaaa cacctgtaaa accaagataa
     1081 aaatactttc ttttctgtga aacgattcgt ttttgaggat gaatataaat aacgtcagtt
     1141 tcgttttcca ttacacacct tctttttcat tttctttaac tataacttga aatgaaagat
     1201 ttggacaact atcatttagg tcaaaactaa aaatgtaagg gaacccattg aatttaacca
     1261 aattttttta attttgcaaa aaaaagttaa agaagactga aaaaaattta aaatataacg
     1321 ttttgagtaa aatgtttgca aaaaatgcta aaaatagggt acaataaaac aacatatact
     1381 gcagtaaaga agtgggggag ttttaatgag aagaaaagtc tataccaagg atcaaattct
     1441 aaaagctgct tatgatgttg ttgcaaagga agggtttaaa ggatttactg cgagaaatat
     1501 tgctaaaaag atggggattt cgacacagcc gatttatctg gaattcaaaa acatggaaga
     1561 tttaaaaaat acgttgctgg attctatctt tgaacaacta aaagaagatg ttttccctat
     1621 tgaacgtact ggtgataaaa tcattgatct cggtttgaac tatatctact ttgcaaaaaa
     1681 gcatcatagt ctttacatgg cattgtacat tgatgaacat ggggacggaa agaaaatgca
     1741 tgatttttca tacaattatt tccgtgaaat agttaaagaa gatgaaagat acaatcaatt
     1801 atcggatgaa tatgtcgatg ctctgcatac tggtacatgg gtagcagtga ctggcttagc
     1861 gtcattgatg tcatcaaaca tcatgcatcc aacagaagaa caaatcattg cactgatcaa
     1921 acggacaatt gactcgattt tagcattaga taatcctgaa ataatcagat agtgttctat
     1981 cagtatgaat ttgatcggta agtccgttgg ctgcaaactg tcaatggact tttttgctaa
     2041 aaaaagcgat ttctgttatt tatctattag aaataggctt gacatttcgt gaaaaaagtt
     2101 taaaaatagt acttacggaa ggttggcaga gtggtaatgc accggactcg aaatccggcg
     2161 aaccggctaa taccggcgcg caggttcaaa tcctgtacct tccttatatc aacgattaga
     2221 ggttgtgaag aagctgtcct gcatcaagta ataagaacgg ccaaacaaaa atagcttttc
     2281 ctattttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtt
//
LOCUS       DANKHJ010000222         2330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000222 DANKHJ010000000
VERSION     DANKHJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            290..697
                     /locus_tag="PW113_003353"
     CDS             290..697
                     /locus_tag="PW113_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527581.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="PW113_003354"
     CDS             711..1112
                     /locus_tag="PW113_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527582.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="PW113_003355"
     CDS             1114..1485
                     /locus_tag="PW113_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527583.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1521..1826)
                     /locus_tag="PW113_003356"
     CDS             complement(1521..1826)
                     /locus_tag="PW113_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527584.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     LHYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          874 a          316 c          393 g          747 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1561 atatagtcat aacgccgatg atcgctacgt gcgtccggca catcagtcac gatatcaaac
     1621 aaaaaatata cgtctttctt cattctagtt ttcgcagcag ggattttaaa gtagttctta
     1681 ttagaataat ggagattgac tagcaggcta gtttcaattg ctaaaaagac tacttcagta
     1741 tcccagactt tatataaatc tttgacaaat ttttctgatg gatcgtattt aaaccaaagc
     1801 agatgttcct ctagctcgat tgtcatgttt ttcacctcaa cgaaagtata cgaactaatg
     1861 tttgttttgt aaaggataaa aatagggtag caaccggtac ccaaactagt acccatttat
     1921 caaaaacgac gaaatttatg taaatagaaa aataagaata aacattgatg tatcaacatt
     1981 tatatatctt tagaacaaat gaaaagggtg gttctcataa gaaaaacatg ttcgcacttc
     2041 taggcaaacc aggatttgaa gaccttgcta aagaattgaa cgaacgcctg taaaataagg
     2101 atttgtcgct agatttaaaa aatcagtgat agaaacatgt ttgctttaaa atgaaactag
     2161 tgatagtttt agtgatagtg aacttaaaaa aagcactcaa tttgagtgct tttttattgt
     2221 tattaaaaat taaaaaacta aaatacatac ccgaatttca tacccgaaat ataaaaaatg
     2281 tcatcacgta catctttttc gaaactaccg aagtagccaa agatgtactg
//
LOCUS       DANKHJ010000223         2317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000223 DANKHJ010000000
VERSION     DANKHJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            289..543
                     /locus_tag="PW113_003357"
                     /pseudo
     CDS             289..543
                     /locus_tag="PW113_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            613..1125
                     /locus_tag="PW113_003358"
     CDS             613..1125
                     /locus_tag="PW113_003358"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527585.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1122..2159
                     /locus_tag="PW113_003359"
     CDS             1122..2159
                     /locus_tag="PW113_003359"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2527586.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          591 t
ORIGIN      
        1 tataaattat ctaaagagaa ttaagttttg attatgtaat aagttttggt actaaaaaag
       61 acttgatcta attagaccaa gtcttttgtt agtgttatat taataacaaa ataaaaataa
      121 gaagtcgctc actccccgac caaagtttgt gagcaaaata aacagtcgat gtttgtttct
      181 taattgtata tcttgatatt aaacgattta agattattct tcaagatata tattcgcaaa
      241 tgtaattgga aaagcgactt cttaaacaaa attaaggagt cgctttttat ggcaaaaata
      301 acgaaaaatg agcaagaaaa tttggtggaa aattacacaa aaaaaccggc gtactctaat
      361 agccggttag acgcacattt cgtgtgcact tctaatccga aattgagctt tgacgctatg
      421 acgatagtcg gaaaccttaa taaaaacagc gctaaaaagc tatccgattt tatgagttta
      481 gatccacaaa ttagactttg ggatatactt caaacaaaat ttaaagctaa agcactacaa
      541 gaccccctat gttaggctag ttgtcgtata ggataataag ttataataaa gactatacgg
      601 aggatgatta ccatgacaaa taaaaacaat gaacgttact ctaccgaact gaaagcgaca
      661 gccatcaaga aaatgatgcc acccgaaaat agggggattc gccaattaag tcaaactttg
      721 ggcattccag aagcgactct ttattcttgg cgtaaaaaag ctagaattca agggaatact
      781 acacccggaa cgaaaaaaaa tgtcgaaaaa tggtcaagtg aagataaatt tttaattgtg
      841 atggaatcat tctcaatgaa ccaagtagaa cttgcggaat attgccgaaa gaagggctta
      901 tttaaagaac aaattgaggc ttggaagaaa acgtgcttat ctgcgaatga acaggaagaa
      961 aatcgaacga gagaacttgc aactgaacta aaagaagaga aaaaacaagc gcgtcaatta
     1021 gagaaagaat tacgtcataa ggaaaaagca ttagcggaag ccgctgctct cttattacta
     1081 agaaaaaaag cacacgcgat ttggggggac caagaggaag aatgatttca tcttcagatc
     1141 gcaaacaagc tgttgaactt attcaagaag ccaatcgcaa tggggcccgt ctcacctatg
     1201 cctgtaatga gctgaatatt agcgttcgaa cctacgaacg atggacgcgt gaaggtacga
     1261 tagcgcatga tcaacgccct ttggcgaaac gtcctgtacc caaaaataaa ctgacggatc
     1321 aagaacgcga aaaaataata gaaaccgttt caaaaaaaga atttatgaat ttgccaccta
     1381 gccaaattgt accaaaactt gcggattgct cgatttatat tgcatcagag tctagcttct
     1441 atcgtgtatt gcgagaaaaa aatatgcaac atcaccgtgg ccgaagtcaa gtttcacaaa
     1501 aacgcatacc gccaagtcac cttgcgacga agccaaatga agtttggact tgggatatta
     1561 cttggctaaa aggtccaata aaaggactct tttaccggtt atatctcatc attgatttat
     1621 ttagccgtaa aatggtaggc tgggagattt gggaatcaga agaagctcgt catgccgaaa
     1681 aacttattaa acagacggtg cttaaggaac gtatccaagg acagccgttg gttttacact
     1741 ccgataatgg cagccctatg aaagccgcga cttttcaagt cttactcgaa aaattaggaa
     1801 ttcaaagttc ttattcaaga ccacgagtga gtaatgacaa tccttattcg gaagcggctt
     1861 tccgaacgtt aaaatatcga ccagaatatc ccaataacgg ctttaaaaca atagatgatg
     1921 ctcgaaaatg gacgaaagag tttgtcgatt ggtataacga ttctcatttg catagtggga
     1981 ttcaatttgt gtctcctgca gcgtgtcatg aaggacgaca tctcgctatt ttagagaaac
     2041 gaaagcaggt ttacgaggac gctaaaaagc gccatcctga acgctggtca aaaggtgttc
     2101 gaaattggac tccacatcaa tcggtggcgt tgaatccttt aaaagaaaca gaaagttaac
     2161 aggagcataa tctttcaaga ttatcaaata accctcgtca cccaataggg gaaaaagtta
     2221 ttcttcccct attggataac gaggccaacc gaccataggg aggttctgct ttttgatagg
     2281 tggaaaataa aaatacgaca actatattga caaacac
//
LOCUS       DANKHJ010000224         2315 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000224 DANKHJ010000000
VERSION     DANKHJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..159
                     /locus_tag="PW113_003360"
     CDS             <1..159
                     /locus_tag="PW113_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2527587.1"
                     /translation="NVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGK
                     RIIKKNHR"
     gene            125..475
                     /locus_tag="PW113_003361"
     CDS             125..475
                     /locus_tag="PW113_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527588.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(567..986)
                     /locus_tag="PW113_003362"
     CDS             complement(567..986)
                     /locus_tag="PW113_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2527589.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(987..1331)
                     /locus_tag="PW113_003363"
     CDS             complement(987..1331)
                     /locus_tag="PW113_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2527590.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1681..2277
                     /locus_tag="PW113_003364"
     CDS             1681..2277
                     /locus_tag="PW113_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2527591.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          651 a          435 c          402 g          827 t
ORIGIN      
        1 aacgtcagca ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc
       61 gttgcaggta tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag
      121 acacttggaa aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc
      181 ccatgaaata actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga
      241 gatacagggt acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa
      301 caatcctcgg gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca
      361 tgatcgggaa gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc
      421 ccagccgatt atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta
      481 ttttctattt ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta
      541 ctttcaggag ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct
      601 gttacaggtt ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg
      661 tcttttgtcg caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc
      721 attaatcgtc ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcacaagt
      781 aagatttgat agccgtctgt atggacatgc cagtgattgc gacaacctgg ttcaaaagtt
      841 acattaccta caccaacaga aacttcggga tcattcacta acatattcag atagctttgc
      901 ccggtaaata cttgagcgta agcttcgttt ttttcacctg ctggaaaaat cacgccattg
      961 tgtatttctt cgtgttttgt catcatttac gcctctttct tatcttcgct attttcttct
     1021 aaatagattt cttttgctaa actaaaagct gaccaagctt ttggccattc tacataaaaa
     1081 gctaaatggg taatgatttc tgctatttct tcactagtaa tgccattttg cttgccaatc
     1141 ttcagatgag ctggcaattg ttcaaaattt cctcctgaaa ttaatgcaga tatggtgatg
     1201 atactacgct gatgagcaga taattcttct ctggaccata cttgtccaaa taaaacatca
     1261 tcatttaatt ctgcaaattt tggtgcaaaa tctcctaatt gatctcgtcc tgcggtttgt
     1321 ttttctgcca tttctcttat tctcccttca cgactgttag gtgattcaac tatggttcta
     1381 aggcaacttt attttctatt gtttcttcgc atttcatttt aaatgtagtc tttcagcctg
     1441 tccaatgctt agtttattca gtcgatatgc tttttaagca tagttgctaa cgcttttttt
     1501 attttaaaaa aaacaagtta ttaaataagt ctttagttgg atgttttttt gagatcaatc
     1561 ttttatactt atgatgaact tacaaaattg tttgttttct gtcatatcaa aaaatggcgc
     1621 agttagttgc ttttctagta aagtatagtt aacaaagaac ctctaaggag ttgatgaata
     1681 atgaatattg ctgcctctcg aaaactgata aatttcatat ccattatcgg attaggccta
     1741 tcaatcgcgc tgacgattta ttttattaat ttaggtgttt tcaaagattt gaatgcgtta
     1801 agaggattag taggtgattc catcatttta ggaccgatta tttttgtctt catccaaatc
     1861 ctgcaagtag ttattccaat cattcctgga gggatcagta ctgcagctgg tgtcctgata
     1921 tttggccctt atgctggatt tatctataac tatattggaa tctgcattgg ttcgattatc
     1981 atttttctgc tagggcgtcg ttatggaaag ccattcatac tttccatgat tagcgataag
     2041 acatataaca aatatattgg ctggctggat aatcaaaacc gttttgagaa gctgtttgct
     2101 ctagctattt ttcttccagt tgctcctgac gatgcgttat gtttgatggc tggattgaca
     2161 aacatgtcag tgaaacgata tacactaatc attttgattg ctaaaccact gtcgattttc
     2221 ttatatagta tggccttgat ttatggaggt caatatctaa gtggtcttct tggataatat
     2281 ttattttgta aaattagtca ttgattgaca atgtt
//
LOCUS       DANKHJ010000225         2299 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000225 DANKHJ010000000
VERSION     DANKHJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..435
                     /locus_tag="PW113_003365"
     CDS             <1..435
                     /locus_tag="PW113_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adaptor protein RapZ"
                     /protein_id="HDL2527592.1"
                     /translation="REKINESFKSKGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPN
                     PHYIPELRPLTGKDQPVYDYVMSFDETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIG
                     CTGGQHRSVALTERLANALGKNYKVNVTHRDKDKRKETVNRS"
     gene            432..1430
                     /locus_tag="PW113_003366"
     CDS             432..1430
                     /locus_tag="PW113_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2527593.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            1464..>2299
                     /gene="whiA"
                     /locus_tag="PW113_003367"
     CDS             1464..>2299
                     /gene="whiA"
                     /locus_tag="PW113_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2527594.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELG"
BASE COUNT          732 a          413 c          529 g          625 t
ORIGIN      
        1 cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg gatcgaattt
       61 gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat ggatgtgcgt
      121 tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa agatcagcca
      181 gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa atttttggat
      241 cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt gacagttgca
      301 atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt ggcaaatgct
      361 ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag gaaagagacg
      421 gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc gtagtggtcg
      481 gcggtggcac tggtctgcca gttatcctga aaagtttgcg aaatcaagga gcagatatca
      541 cagcagtagt gactgttgca gatgatgggg gaagcagcgg tgcaatcaga gaatcgatcg
      601 ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat atgccacaat
      661 tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta gccaatcata
      721 cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact tatgaggcga
      781 ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct tcagaaactc
      841 ctttgacctt gcatgctgtc tttaaagacg gaacgactgc agtaggagaa tctaaaatcg
      901 cgatcgaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat aaaagtgaag
      961 ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc gttttgggac
     1021 ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc ggcgaagcaa
     1081 tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa aaaggggaaa
     1141 cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg aatcagcctt
     1201 ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg gacccagaga
     1261 tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga aatgaaggat
     1321 gtcgagtgat ttccaccgac tttttggaat tgaaagatgg tggtgtcttc cacgatggag
     1381 acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa gcaatttatt
     1441 gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa gaactaactg
     1501 gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg cgaatgaacg
     1561 gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa aatgctgcaa
     1621 ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt gagctactcg
     1681 ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg aagcaaggca
     1741 caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat ggacacgttt
     1801 cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt gcttttatgg
     1861 cctcaggttc agtcaataat ccggaaacga gccgttatca tttggaaata ttttctattt
     1921 atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta catgctcgaa
     1981 cgcttgaacg gcgcaatggc tatatttctt atttaaaagg agcagaaaaa attgctgatt
     2041 tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt cgaatcgtac
     2101 gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat ctgaacaaga
     2161 cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca aaggtcggtc
     2221 tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa catccagagg
     2281 tcagcttgaa agaattagg
//
LOCUS       DANKHJ010000226         2253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000226 DANKHJ010000000
VERSION     DANKHJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1087)
                     /locus_tag="PW113_003368"
     CDS             complement(<1..1087)
                     /locus_tag="PW113_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2527595.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVD"
     gene            complement(1179..1769)
                     /locus_tag="PW113_003369"
     CDS             complement(1179..1769)
                     /locus_tag="PW113_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2527596.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            1948..>2253
                     /locus_tag="PW113_003370"
     CDS             1948..>2253
                     /locus_tag="PW113_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2527597.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     "
BASE COUNT          720 a          419 c          421 g          693 t
ORIGIN      
        1 agtccacata gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac
       61 gagcttcttt taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa
      121 tgatcgcact ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag
      181 tatcgcagat agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat
      241 ctgaacggat cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct
      301 ggaatagatc ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa
      361 ctgtttttga attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat
      421 gttcgatcgg actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca
      481 agccgtatac gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca
      541 taccattttc aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca
      601 aatgggattt gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg
      661 tttttttatt tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac
      721 cttttgttcc agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca
      781 tagcaagaag cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt
      841 cgtatggttg ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt
      901 cttctttgaa attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat
      961 cataacttaa ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg
     1021 aaacaaattc ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga
     1081 agctcattga ttgatccctc gctttattca atttcttggt ttattatagc acactctttt
     1141 taatatatcg tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca
     1201 ggggacaagc ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg
     1261 aatagtgtct ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta
     1321 agtaaaagtc cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc
     1381 gttataccgc catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa
     1441 agacttcctg aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc
     1501 tctaaaacaa caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct
     1561 aataggacag cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt
     1621 tggatagaaa aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata
     1681 tcgtgaggga gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata
     1741 acgggtagtt tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag
     1801 gctgtatatt tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct
     1861 tataaaagtt atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat
     1921 gttttgtaga aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa
     1981 ttaacaccgc aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt
     2041 tcaagattgc taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct
     2101 aaggctgcca atggattatt caatatggga tacaatatat atgcattgtt tctactgatc
     2161 tctacagcag gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat
     2221 gaatatggca cttcacgacg attatttatc cgt
//
LOCUS       DANKHJ010000227         2249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000227 DANKHJ010000000
VERSION     DANKHJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(136..2169)
                     /gene="brxL"
                     /locus_tag="PW113_003371"
     CDS             complement(136..2169)
                     /gene="brxL"
                     /locus_tag="PW113_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL2527598.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT          723 a          492 c          303 g          731 t
ORIGIN      
        1 tgattctttt tggaatcatg atcaaggacg gacatacaca aaaatatttt gcccattcga
       61 tccgaataga ttaacgacta atctagtaag taatatagat tggtacgtag tcaaaccagc
      121 aaatggaaaa gttgtctatt ccaccccaag ggctttaaag acagcatctt ccggcgtttg
      181 ataaaaaatc aaattaaagg tacccattaa ttctgggggt acagttccaa tatcagcagc
      241 gcttatcata ggtacaagta cttttttcgc tccggcatct tttgctactt gtaaacagtt
      301 agctaaatta tctaccttaa tcatagaacc accaattgta atatccccta aaataactaa
      361 acttggtaat acaggtttat ttaaggaaac tgagcataaa gctattatag ttgataacga
      421 taatgttttt gtcataccaa tgccattcat atcttgaaca tgaacgacaa aatcttttgt
      481 cgtcaaacta atagactgac taacacttcc ataattagct cttaaatatc ggtatgccga
      541 atccatcgct tctcttgctc cagcatctga attgatccca gtacgttcaa actttccatt
      601 tcccgaagtc atttgcgttt ccaatttata ggtacctaat tgcccagttt tgccataaga
      661 taccgtgtac aaatgccctg gcttccctaa cccttctgga atcaaagttc ctccaccttg
      721 ttcaggaaca gaaacatact cttctcgctc ttcttctaaa tcaatgtatg aaaaattgac
      781 atcataaaat tccattccac caattttctt taattgttct tttacacgac gacgcatttc
      841 aagagcaaat cttagtattt cttcgacatc acttttttca aatctaccat caggatacaa
      901 cagtttcatc atcccagata caatccgacg aactgcaatc gtatcccgct gatttaattg
      961 agaaccaagt ttgaaatatt tatcaaaact atcgccaaaa ctaacttttc gaagctcacg
     1021 catgacttca gaaaaataat ctgtaatgaa gccatagtca tttgtaaaaa aatctggtcg
     1081 atatttagga atttcccaac ctggtaaata acaatgcatc cgatccaaaa aggctgaatc
     1141 cgtccccata acctccggaa atggctcaaa taaattcgaa gttttcaata ttacatcaac
     1201 gctttgatta atatttccta caaaagccat tgatgctgaa gcattctttt cttctttgcc
     1261 tctagcaaaa gatcctgaag ccatgtaatc tttcatgatt tgaacgccat cattatcctt
     1321 aaatttaatc ccagcaactt cgtcaaaagc aacacagtcc cacataccta caagtcccat
     1381 tgtacgacta gccatattat agaaaaggtt tgctactgtt gtttgtccac ctgaaacaag
     1441 gattgagttt ggtgaaattt ctttatatac atgcgattta cctgtaccac gtggtcctag
     1501 ttcacagaga ttaaaattat tttcaattaa tggaataagt cttgctaaat gaagccattt
     1561 taccctttta ttaaattgtg taggttccat accaatggac cgtagaagta agtcaatcca
     1621 ttcatttgta gtaaactcct tgcgagccat cttgtaagcc tcaatgtcta aaccaggcat
     1681 ttggattgga tcaacctttt ttgcaataaa agggctattc caacgatcat tctcatcata
     1741 ccaatattcc atcgaaacaa tacaccagat ccccccacct aataatcgat caaatttttt
     1801 cggatactct tcactaattt gaacattttt taatcccagg ttagagaatt ctgcttcata
     1861 acaatctttc ttttcattta aatgtacagt tactttatca ataactgtat aaccacccaa
     1921 ttcacgaatt tttgctttta ctttttctgc ttcatcagga cgcacatagt ttcgagcaag
     1981 aatattttta acattttcca cacctttttc aaccaatgtt tcatccaatg acgaagcata
     2041 cataccaagt aaatattcca atacataaac aggtacattc gcaccttctt taattttctt
     2101 tgttaaatct tttctgacaa tctttccggg aaagaattca gataacttta aaatttcctg
     2161 ttgttccact agaattcctc catgtgttca gtcattataa tagcaattaa agtatatcat
     2221 acttaaccag taggcttgat aatacaaaa
//
LOCUS       DANKHJ010000228         2207 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000228 DANKHJ010000000
VERSION     DANKHJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>2207
                     /gene="nifJ"
                     /locus_tag="PW113_003372"
     CDS             <1..>2207
                     /gene="nifJ"
                     /locus_tag="PW113_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2527599.1"
                     /translation="GFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVS
                     GTNQNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIV
                     SMGSVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKE
                     PGAGGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKK
                     RFTIGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDK
                     YAQGYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLK
                     PGGIFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAME
                     TAFFKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHW
                     RTLEMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRG
                     VALEVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKY
                     RIQVSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKN
                     TVLSTQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASP
                     YTTNECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELR"
BASE COUNT          687 a          421 c          511 g          588 t
ORIGIN      
        1 ggattccgta cgagtcacga gattcaaaaa atcgaagttt tggaatatga agaattagct
       61 cctctgatca atcaggagaa attagcttca tttcgtaaac gagggatgaa tcccaatcat
      121 ccgactgtta gtggtacgaa ccagaatcca gacatccatt ttcaacaacg ggaaacgatc
      181 aacccctatt atgataaatt acctgaaatc gttcaaaaat atatgaaaga gatcaaccga
      241 ttacgaggaa cttcttatga cttggtgact tattacggag cagacgatgc ggaagaagtc
      301 attgtttcta tgggatcagt tgcacagacg attgaacaaa cgattgatta cttgaatgca
      361 aacggacgga aaacaggttt tttgaatatc catctttatc gtccttttcc agtggaaaca
      421 ttcgtgcaaa agttgcctca tacagtaaaa acaattgctg ttcttgatcg aagtaaagaa
      481 ccaggggcag gtggagaacc tctcttatta gacgttcaaa gtgcgttata tgattctgaa
      541 cttcgcccgg ctgttattgg tggtcgctac ggattggggt ctaaagatgt gactcccgac
      601 caaatctcag cagtttttga tgaattgaaa aaagacccaa gcatacgcaa aaaacggttt
      661 accataggga tcgtggatga tgtgacttat caatctttgg aaccaaagga aagtctggat
      721 ttgacagaac cgcaaacatt ccaggccaag ttttggggat ttggatctga cggaacagtt
      781 ggagcaaata aatcagctat caagattatt ggagatcaca ccgataaata tgcgcaaggc
      841 tatttttatt atgattcgaa aaaatctggc ggtctgactg tctctcatct tcgttttgga
      901 gatacgccga tacgttcagc gtatcttgtc gagcatgcgg accttgttgc ctgtcacaca
      961 ccagcttacc tgcatagcta tgacttggtc aaaggattga agccaggtgg cattttttta
     1021 ttgaatactc tttggagcga cgaacaatta gaaacgcatc tgcccttaaa attaaaacgt
     1081 tacttggctg aaaataacat tagattctat acgatcaatg ccatgagatt ggcccaagaa
     1141 gtcggtttgg gacgccggat caatacggca atggaaacgg ctttttttaa attagctgac
     1201 atcattcctt ttgatgaggt gcttccgctg ctaaaagaag aagccctgaa aagctatgga
     1261 cataaatcaa tggctatcgt agaaaaaaac atacaagcaa tcgatcggac gaccgaactg
     1321 cttcatcaag tcgcgatccc agaccattgg agaacgttgg agatgcctga aaaaatcaat
     1381 aaaaggaaca ctggttatat acaagaaata cttgaaccag tgaatgcaca ggaaggaaat
     1441 caactctctg ttggtacact tgtagcaaat ggaatgacaa ctggcgaaat gcctttagga
     1501 acggcttcta tggaaaagcg gggcgtcgct ttagaagtac ctgagtggat tagtgatcgc
     1561 tgtacgatgt gtaatgaatg tgcctttgtt tgtcctcatg ctgccatccg tccattttta
     1621 gcagatgaag aggaaatgga agaagcgcca gaaggattta tcgtgagaga aatgcgaggc
     1681 gcagacggcg taaaataccg tatccaagtt tcagtggaag actgtacagg ttgtggtctg
     1741 tgtgtagagg cttgtcccgc aaaagaaaaa gctttagtga tgaagcctta tgaagaacag
     1801 aaagaacaag ctgtgaattg ggcttttgca atgacattaa gacaaaaagc aaatccagtc
     1861 aaaggaaaga atactgtctt gtctacacaa ttcaatcaac cgcttcttga attctcgggc
     1921 gcttgctcag gctgtggcga aacaccttat gtaaaattat tgacacaaat gttcggtgat
     1981 cgaatgatga tcgcgaatgc gacaggctgt tcttctattt ggggcggtgc ttctcctgct
     2041 tctccttata cgaccaatga atgtggtcaa gggcctgcat ggtcaaattc tttattggaa
     2101 gataatgctg aatttggtta tggtatgctt ctagcggcac agactcgtcg cgaggctttg
     2161 gcaagttcaa tcaaagaagt attgccaaaa gtatcagaag aactacg
//
LOCUS       DANKHJ010000229         2207 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000229 DANKHJ010000000
VERSION     DANKHJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..2157
                     /locus_tag="PW113_003373"
     CDS             <1..2157
                     /locus_tag="PW113_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2527600.1"
                     /translation="YYLTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNS
                     LGEKPFTEWQKERTMITTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTD
                     IPAHIKEQELVLPFKKKNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVG
                     IADISVLNEKQEIIENAHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLM
                     EGLEAKNPIFMMSDSGARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLE
                     MFISTHGARKGMTDTALKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGN
                     EIIESLEDRLVGRYTRKEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFT
                     CNTKHGVCKHCYGRNLATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDI
                     TQGLPRVQEIFEARNPKGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPY
                     TARMKVAEGDMIHRGAPLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGD
                     KHIEVMVRQMLRKVRVMDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLG
                     ITKASLETNSFLSAASFQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRY
                     RDMEPKEVGVASENVYSISDIEAQMAAEDAMNNQTK"
BASE COUNT          724 a          451 c          482 g          550 t
ORIGIN      
        1 tactacttga caatggaaga agaaggacgc gaaggcgaag gaatggtctt cagagatcaa
       61 aacgaagccg ttatcgcatg gcgcaacgga tatgttcatt tacattcacg tatcggtgtg
      121 aaccctaact cattaggtga aaaaccattt actgaatggc aaaaagaacg tacgatgatc
      181 acaacagttg gtaagatcat cttcaacgag atcatgcctc ctgaattccc ttatctgaat
      241 gaaccaacag acttcaactt gaccgtccaa acaccggaca aatactttgt tgaagctggt
      301 actgatattc ctgcacatat caaagagcaa gaattagtat taccgttcaa gaagaaaaac
      361 ttaggaaaca tcatcgcaga agtcttcaaa cgattcaaag taacagaaac ttctaagatg
      421 cttgaccgta tgaaagactt agggtacaaa cattcgacac atgccggtat cacagtaggg
      481 atcgctgaca tcagtgtact gaatgaaaaa caagaaatca ttgaaaatgc ccataaacaa
      541 gttgaaacta ttacaaaaca attccgtcgt ggtctgatca cagatgacga acgttatgaa
      601 cgtgtcattg ctgtttggaa tgctgcaaaa gacagcatcc aacaaaaact gatggaaggt
      661 ttggaagcga aaaatccaat cttcatgatg tctgattctg gagctcgtgg taacatttcc
      721 aactttacac agcttgccgg aatgcgtggt ttgatggccg ctcctaatgg tcgaatcatg
      781 gaattgccaa tcatttcgaa cttccgtgaa ggactatccg tcttggaaat gtttatctca
      841 actcacgggg ctcgtaaagg gatgaccgat acagccttga agactgccga ttcaggttac
      901 ttgactcgtc gtctggttga cgtggcccaa gatgtaatca tccgtgaaga agattgcggt
      961 actgaccgag gtcttgaaat ccaagccatc cgcgagggaa atgaaatcat cgaatcatta
     1021 gaagatcgtt tagtgggacg ttacacccgt aaagaagtac gtcatcctga aacaaatgaa
     1081 ttgatcattg caggaaacca attgatttcc gaagacatcg caaaacaaat cgttgatgca
     1141 ggtgtcgaaa cagtgacaat ccgttctgtc ttcacatgta atacaaaaca tggtgtctgc
     1201 aaacattgtt acggacgcaa ccttgcaact ggctctgacg ttgaagtcgg agaagcagtt
     1261 ggtacgatcg ccgctcaatc aatcggtgaa cctggtactc agttgacgat gcgtacattc
     1321 catacaggtg gggttgccgg agacgatatc actcaaggtc ttcctcgtgt ccaagaaatc
     1381 tttgaagcac gtaatccgaa agggcaagca gtgatcacag aagtaacagg ggatgtcatc
     1441 gatatctcag aagatcctgc aacacgtacc aaagaagtaa caatcaaagg aaaaacagat
     1501 acacgtacgt atactgttcc ttatacagca cgtatgaaag tagcagaagg cgatatgatc
     1561 caccgtggtg caccattgac agaaggatca atcgatccaa aacaattgtt gcaagttcgc
     1621 gatgtattat ctgttgaaaa ctacttgctt cgtgaagtac aacgtgttta ccgtatgcaa
     1681 ggggtagaaa tcggcgacaa gcatatcgaa gtaatggttc gtcaaatgct gcgtaaagtt
     1741 cgtgtaatgg atccaggtga tacagatatc ttaccaggta cattattaga catcgcagac
     1801 tttaaagatc aaaactacaa tacgctagtc tcaggaggcg ttcctgcaac ttctcgacct
     1861 gtcttactag gtatcacaaa agcatcatta gaaacaaata gtttcttatc tgctgcatca
     1921 ttccaagaaa caactcgtgt cttgacggat gctgcgatcc gcggtaagaa agatcctctt
     1981 cttggtttga aagaaaatgt tattataggt aagatcatcc cagctggtac tggtatgcaa
     2041 cgttatcgtg acatggaacc aaaagaagtc ggtgtagcaa gcgaaaatgt ctacagtatt
     2101 tcagatattg aagcgcaaat ggctgcagaa gacgcaatga acaatcaaac aaaatagata
     2161 ttttaaaaaa gaggttgtga caaattattg tcacaacctc ttttttg
//
LOCUS       DANKHJ010000230         2164 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000230 DANKHJ010000000
VERSION     DANKHJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1400
                     /locus_tag="PW113_003374"
     CDS             <1..1400
                     /locus_tag="PW113_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2527601.1"
                     /translation="YFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIACLLAIYYRKY
                     SSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLGQDFSFKEAKK
                     LKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAINDRSSGEFGRLM
                     KQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYTTHAWTQGGKV
                     KELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGTIKKKKEFYLM
                     GSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQAQELLNLPLMD
                     NWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMILGTAFAKADR
                     AYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVPLTQFIVVTSM
                     LVFSKLNLKKALSSK"
     gene            1657..>2164
                     /locus_tag="PW113_003375"
     CDS             1657..>2164
                     /locus_tag="PW113_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527602.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLT"
BASE COUNT          686 a          409 c          425 g          644 t
ORIGIN      
        1 tctattttta ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacgt
       61 atcgtaatgc cctcgtgatt ccggctgttg agatcattat cgcttgttta ctagctattt
      121 attaccgtaa atatagttcc agcaaaaaaa tgctgccgtg gtttttaggg ctagctgttt
      181 cttttccttt cttttggttc attcgcgaag attccatctg gcttcttcct tttactgtag
      241 tcgcgttagg catcagtttc gtccagatct tgctaggaca agacttttct ttcaaagaag
      301 caaagaagtt aaaagaacga ccatttctcc tttcgaaaca agaatggctg aaactttcat
      361 tgtttttact ccctctagtc ggtttctcca tcgtctatca gtggatcgaa tataaaaacg
      421 agacaacata tagtattgcc gccatcaatg accgttcgag tggcgaattt ggacgtttga
      481 tgaaacagct gatccgaata gatgacgaaa cgaacttaaa taaagaaaac agtgagatat
      541 gggtctcgca tgctgcatta aataaagcat tagacgcatc tcctacattt catgctgttt
      601 ctactcgtat tgacgaattg tatacgactc atgcgtggac ccaaggtgga aaagtaaaag
      661 aattaccagg agatatcatt ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt
      721 accaaaacaa tgcaaaagaa accaatgatt tttggaaaaa agtcaatcaa gaattagaag
      781 aagcctatca aaagggaact atcaaaaaga aaaaagaatt ctatctgatg ggatcagggg
      841 atggaaagac gatgaatgat ctgccaatcg tatttgcttt tctgaaatca ggatatgcct
      901 atgcatggcg atacaaagat ttccatcaag gggggaatta tagtttcggt acgcaagaac
      961 aagtcgaaca ggcccaagaa ttattgaatc ttccactaat ggataattgg atcgatagca
     1021 gtgatccgag aacacctgat ttccagttga caaaaacagc aaaagtcgcg aatgtcatga
     1081 taaaaattta ccaagttctg gccattcccg tgctgattgc ttcaatagca gggatattac
     1141 ttatgatact aggaaccgca tttgcaaaag ctgatcgtgc ctattatagt tcctctttga
     1201 ttgtagtcac cggattaata ctgacacaat tggtttttct tttcggcgtc tcgtggtttt
     1261 gttcttatgc gccagaaaaa aaggattatt ttttaagtgt ctacactggc gcaggcgtgc
     1321 cattaacgca attcatcgta gtaacaagca tgctagtttt cagtaaattg aatctgaaaa
     1381 aagctttatc ttccaaataa aaacatacta agattagagg agaacatcat tgacgaatga
     1441 tgttctcctc tattttctat ttttagatta tagtgaagag tgaaagaaag ttcgaacaaa
     1501 gccttgagga ctatgagtcc ctatcaaaaa ccgaacttct ttactgtcat tttagaatca
     1561 ggtttacgta tgttggggcc atgagcccgt gtataggaaa ttggaatcga aatgaagcag
     1621 taaagttctt tcaaaactta aaaaggagga aaacaaatgc cttacattct tgcattagat
     1681 gtttcaatgg gacacagcta cagtgtccta tacaaagacg acacctgctt atttgaagac
     1741 gagatcgaac acactcaaag aggatttcat gcattactcg aagagatcca gcacttgcct
     1801 gaacgtccat tgattgtttt tgaagcaacg gggatttatt ccagaccagt cgaaaagttc
     1861 tttcaagatc atcacttttc ttactgtttg ttaaacccgt tagaagcgaa aaaacagatg
     1921 gaagaaaaca ccttatgcag ttggaaaaca gataaatcgg atgcccatcg gctcgcacag
     1981 actcatctta aaaatcaacg tcagccaaaa gaagtacaaa gcaattttta cttagagatg
     2041 cgcgatttag cccgttttta tcaagaaatt gagaagaaaa tcactcgtct gcgcatggat
     2101 ttacacaact gccttcagtt gacttttcca gagcttgaac agttcttttt aaacaggctg
     2161 accc
//
LOCUS       DANKHJ010000231         2133 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000231 DANKHJ010000000
VERSION     DANKHJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1008
                     /locus_tag="PW113_003376"
     CDS             <1..1008
                     /locus_tag="PW113_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDL2527603.1"
                     /translation="KNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTV
                     LVQRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQ
                     AENLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPG
                     FFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGC
                     FNCLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFH
                     KVNQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELD
                     D"
     gene            1001..1462
                     /locus_tag="PW113_003377"
     CDS             1001..1462
                     /locus_tag="PW113_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDL2527604.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            1475..>2133
                     /locus_tag="PW113_003378"
     CDS             1475..>2133
                     /locus_tag="PW113_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527605.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     E"
BASE COUNT          744 a          271 c          416 g          702 t
ORIGIN      
        1 aagaatagac ataatgtttt acttattcta gatatcccaa ttgaagaaaa gtcaaataat
       61 gtcataattg gatttctttt tacaaaacaa tctggatcta taaaagatta taagggtacg
      121 cctaggactg tattagttca gcgtcatgat gaattatatt tggtgaatag aacgagcgga
      181 gaaaactatg gattgggtgc taatatagct attgttgggg tagggtcagt tggcagtaaa
      241 attgctgaaa atcttggtcc attaggtgtg agaaagatga cactcataga caatgattgt
      301 tttcaagctg aaaatttgta tcgaaattca cttggtgcag atagtttaaa aaatgcccga
      361 tctcagaata atttttttaa agttgattta atgcaggagg agctcgaaaa aaaaatccca
      421 tacctttgtg ttgaagtgga gtcaatgaac gttttagact taattagtat gcaacctggt
      481 tttttcaagg aatatgacta tgtttttgtt tgcataggag atacaatggc aagcctggct
      541 ttaaataatt atttttccaa aagtagtcaa aaagttttct attcttggct ggagccatat
      601 ggcattgggg gacacactct atttataaat tattctctaa atcaaaaagg ttgtttcaat
      661 tgtctttata cagattcaaa ctctaaaaat aggtgttcaa atagagcttc tctggtaaaa
      721 gaaggacaaa tttttgaaag gactttagcg agttgccgct caaaatttat accatataat
      781 gctattgcct cgacggaatg tgcaattaat gcaataaaac ttttccataa agtcaatcaa
      841 aataatatga ccaatagtac tcttttttca tggataggag atgggagtac ttttgagaat
      901 ttaggatatc aatatagcga cagaaatact tttcttaatg gagtcaataa gtttgaagaa
      961 agattctttt ctaaatattg caaagtatgt ggggagttag atgattaaat tttcgtgtga
     1021 ccgtattgtc attgaagtaa gggatgaagt tcttaagata tttgaacaat atcggcaggg
     1081 aaaggatgac ttgcctgaag ctggaggagt tcttcatgga aaaagactta agaatgggag
     1141 cataataatt accgatataa caacacctca aataggagat atacaaaaaa gattactctt
     1201 taaaaaaaac cgagaaattc atcaacagct ttctaatgag aaatggatag aatcaaataa
     1261 gttttctatt gcattgggag aatggcatac tcatcctgaa gctgtaccaa ctccatcaaa
     1321 tgttgataag aaaagctgga gactgaacgt gagcaagcaa catgatgata gagtttatgt
     1381 ttttattatc gttggatttt ctgatttgag aatgtggtta ctgtcaaaaa aatcagatat
     1441 taggcaggct ataaaactat aaaggagtga ttaggtgaaa tcacaaaaga atatttggtg
     1501 gtgggggttt ttagtaggaa aggtaatgat accagccctt ctcttaattt ctctaccgat
     1561 gattactttt ttctttacca ataaaaaaat gagtaaagaa gccgtgattt ttttctttgg
     1621 agaccaaaaa gctaccttta ttgaaacgtt agtaacatct cttcagctta atttgaatta
     1681 cattttttca attattattg ttatatcgtt attgaatttt tttaaaaaaa gaaattcagg
     1741 aaaggtgttt aactctaatg gaaatgtgta ctacaactat ttttattttg tattttgggt
     1801 agcagcaact ttacttggtt atgataaaat tcagattgca ggaattccta tacacatgca
     1861 atataagttg gttttgagtg gaattttttc tgaggtgttg ccagatatat acgatgatca
     1921 ttacgacagt gatggaacat gtaaagtaag cattgaaaaa gaaaatttcg atgacatcga
     1981 tggatatgac tctgtgaact tactgattat tgatacttac gatataaaaa tgagtgaatt
     2041 atctatggaa aatcaaacat atcctacgat aattgttaga gggaattcaa tagatggagt
     2101 tcgtaaagtt aatagatctt taatcttgga aat
//
LOCUS       DANKHJ010000232         2111 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000232 DANKHJ010000000
VERSION     DANKHJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>2111
                     /locus_tag="PW113_003379"
     CDS             <1..>2111
                     /locus_tag="PW113_003379"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2527606.1"
                     /translation="KWGHRAIAITDHGGAQAFPEAHSAGKKAGVKILYGVEANVVDDG
                     VPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKMYKGNVIESFDEFIDPG
                     HPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVAHNAAFDMGFLNTSYAR
                     YGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQHHRAIYDAEATGHLAW
                     IFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKNQAGLKDLFKLISMSNV
                     EYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVEEARNRAKFYDYIEVMP
                     KAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGNVHYLNEEDAIYRKILI
                     NSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVVENTNKIADICEEVIPV
                     KDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEKELNSINGNGFSVIYLI
                     AQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHYYCPECQYSEFFEDGTY
                     GSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDIDLNFSGDYQAEAHNYTK
                     VLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEVDRLAKGATGVKRTTGQ
                     HPGGIIVIPDYMDVYDFTPIQ"
BASE COUNT          693 a          373 c          490 g          555 t
ORIGIN      
        1 aaatggggac accgagctat cgccattaca gaccatggtg gtgcgcaagc ctttccggaa
       61 gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc
      121 gtagatgatg gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca
      181 tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag
      241 ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat
      301 ccaggacatc cattgtcacg aacaacggta gatctgactg gtatcacgga tgaaatggtt
      361 cgagggtcaa agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca
      421 attttggtag cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg
      481 tatggtattc ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg
      541 tatcctcaat tcaaacgttt cggattaggt gtgttatcta aaaagtttgg tgttagcttg
      601 gaacagcatc accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt
      661 gtgaaagagg caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc
      721 ggagaaggag attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat
      781 caggcaggac tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa
      841 cgcgtcccac gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc
      901 tcagcttgtg ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca
      961 agaaaccgag caaaattcta tgattatatt gaagttatgc caaaagcggt ctatgcgcct
     1021 ttgatcgagc aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg
     1081 gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg
     1141 aacgaagaag atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg
     1201 ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca
     1261 tttcactttt taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc
     1321 gcagatatct gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca
     1381 ggttcagaag atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat
     1441 ccgttacctg agatcattca aaaacgatta gaaaaagagt tgaattctat taatggtaat
     1501 ggcttctcgg ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc
     1561 tatctagtag gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc
     1621 actgaagtta atccattagc ccctcattat tattgtccag agtgccaata ttccgagttt
     1681 tttgaagatg gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt
     1741 ggtgcacgct taaacaaaga cggtcatgat attccatttg agacattttt aggattccat
     1801 ggagacaaag tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat
     1861 aactatacga aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca
     1921 gtcgccgaca aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag
     1981 tttcgaagtg cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc
     2041 ggccagcatc caggggggat catcgttatt cccgattata tggatgtcta tgattttacg
     2101 ccgatccaat a
//
LOCUS       DANKHJ010000233         2102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000233 DANKHJ010000000
VERSION     DANKHJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            236..1207
                     /locus_tag="PW113_003380"
     CDS             236..1207
                     /locus_tag="PW113_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2527607.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1231..>2102
                     /locus_tag="PW113_003381"
     CDS             1231..>2102
                     /locus_tag="PW113_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527608.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPL"
BASE COUNT          739 a          369 c          464 g          530 t
ORIGIN      
        1 agagaatacc tctgaatgaa acggaatatt ttgttctcca ctttcatata acttacattc
       61 tcagattctt tttatcaaaa cgagaccttt agcgaatgga aacagctatt ttttaacgtt
      121 tcttggtacg tatggttaaa cgactctttt acaacctttt cttatttgct atatagtttc
      181 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
      241 ggaagagtct agttctttag agattagatt gacagctgct gatgatgtca gcatgctttt
      301 aggtgcacac gacaagcata taaaactgat cgaggaaaca acggaaacaa ccatccatac
      361 acgtggagaa atcattcaga tcattggtga acaatcccag atttccttgg ctgcctcagt
      421 cattcggaca ttacaggaat tgatcaaacg aggaatccac gtaagtacac cagatgtcgt
      481 gacggcattg aaaatggggc aaaaaggtac attagattat tttgttgaga tgtatgagga
      541 agagatcgtc aaagaccgta atggaaaacc tatacgggta aaaaacagcg gtcaaaagaa
      601 atacgttgaa tcggtagcca aacatgacat cgtatttggt gtagggccag ccggaacagg
      661 gaaaacgttt cttgccgtag tattagcaat tgctgcccta aaaaaaggcg aagtgcaaaa
      721 aatcattttg acgcggcccg ctgtagaggc aggggaaaac ctaggctttc tacccgggga
      781 tctgaaagaa aaagtggatc cttatctgcg gcctgtttat gatgctttat accaaatttt
      841 tggattggat catacgaacc gattgatgga acgaggcgta atcgaaatcg caccattagc
      901 ttacatgcgg gggcggacat tggatgatgc attcgttatc ttggacgaag cacaaaacac
      961 gacagtcgct cagatgaaaa tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt
     1021 caatggggat acaagtcaga tagacttgcc tcgagggacg acaagtggtc ttgttcatgc
     1081 agaacgaaca ctaaagcaaa tcaaaaaaat cgattttgtt aattttgaag caagtgacgt
     1141 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcgg atcttcatca
     1201 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
     1261 gaaaaactcg gaaaggcagc attgcctttc cttctgttct tgttttttat cattgtctgc
     1321 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
     1381 gaagagagta tccgtgcgaa taaaacgatc gagaacacgg aagaaaccga acaaagaaga
     1441 aaactggcag cagaagcagt gactccagaa tatacttatc aaaaagatct ggcagatgac
     1501 cagaacaatc ggatcaagca attattcgaa ttgatcgata agacgaatga taccatcaac
     1561 aaatcttata atgacaaggt agataaggcg aaggaagatg aaaatatccc gaaacctact
     1621 acagaagagc ggattgctac actgaagaaa aattttgaaa acgtgaatgc agaaaacgtt
     1681 gcgttttacc aaaaactgcc gaacaatttt tattcgacga tatttgaaat gactgaaacg
     1741 gaaattcaca cggttaggga tgaaagtttg aagctcattg acgaacagat gagtaaacaa
     1801 gttcgtgaaa gcgaattgga agcttttaaa caagaagcag aagatcagat tcagtatttg
     1861 aatgtcacaa cagagcagca acaaatgatt cgctacttag tggatcaagg cattgtagtc
     1921 aatgatgtat tgaatgagaa aaagacagaa gaattaaaac aatctgcacg agaagctgta
     1981 caaccagtga tgatcttcca aggagaaatc attgttcgcg aaggaaatca aattgatgct
     2041 tcagcgatga aaaaattgga attactagga ttgacgaatc aaacgacttc gattttccca
     2101 ct
//
LOCUS       DANKHJ010000234         2079 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000234 DANKHJ010000000
VERSION     DANKHJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            220..837
                     /locus_tag="PW113_003382"
     CDS             220..837
                     /locus_tag="PW113_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2527609.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            878..1177
                     /locus_tag="PW113_003383"
     CDS             878..1177
                     /locus_tag="PW113_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2527610.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1211..1378
                     /locus_tag="PW113_003384"
     CDS             1211..1378
                     /locus_tag="PW113_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2527611.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1423..2028)
                     /locus_tag="PW113_003385"
     CDS             complement(1423..2028)
                     /locus_tag="PW113_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2527612.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          676 a          345 c          396 g          662 t
ORIGIN      
        1 tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg
       61 ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt
      121 tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt
      181 tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc
      241 cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga
      301 atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa
      361 tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat
      421 agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag
      481 cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta
      541 gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa
      601 gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag
      661 ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct
      721 gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga
      781 aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag
      841 atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt
      901 cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc
      961 tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca
     1021 gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga
     1081 cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat
     1141 tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag
     1201 gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa
     1261 tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca
     1321 aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa
     1381 acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt
     1441 ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg
     1501 aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt
     1561 tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa
     1621 tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag
     1681 cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc
     1741 taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt
     1801 attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga tgacgcaatc
     1861 accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat ctttaccact
     1921 ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca attgtcgatc
     1981 caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa aaaactcctt
     2041 tttaaaagtg atataattta catgtaaaat ttacttgta
//
LOCUS       DANKHJ010000235         2056 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000235 DANKHJ010000000
VERSION     DANKHJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            493..714
                     /locus_tag="PW113_003386"
     CDS             493..714
                     /locus_tag="PW113_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527613.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="PW113_003387"
     CDS             715..972
                     /locus_tag="PW113_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527614.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="PW113_003388"
     CDS             complement(1108..1710)
                     /locus_tag="PW113_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2527615.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="PW113_003389"
     CDS             complement(1777..1932)
                     /locus_tag="PW113_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2527616.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DANKHJ010000236         2030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000236 DANKHJ010000000
VERSION     DANKHJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..622)
                     /locus_tag="PW113_003390"
     CDS             complement(<1..622)
                     /locus_tag="PW113_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2527617.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLG"
     gene            complement(619..1608)
                     /locus_tag="PW113_003391"
     CDS             complement(619..1608)
                     /locus_tag="PW113_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2527618.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(1605..>2030)
                     /locus_tag="PW113_003392"
     CDS             complement(1605..>2030)
                     /locus_tag="PW113_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527619.1"
                     /translation="GLAGFRMRDIATLSGGQTQRVWIAMALAQETDILILDEPTTFLD
                     PAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKLITEGTPEEVIT
                     KECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
BASE COUNT          722 a          467 c          353 g          488 t
ORIGIN      
        1 ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg
       61 caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt
      121 ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata
      181 agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag
      241 cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg
      301 aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac
      361 tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac
      421 aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg
      481 ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag
      541 gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataga
      601 acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac
      661 gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc
      721 tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata
      781 acgatagtcc gttccaacca ttccgcgaac aactggcgga accaacagcc caataaatga
      841 aacaggtccg actaacgaaa cggcaatggc tgctaacact gcaactagac agaaaacaat
      901 caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat
      961 caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa
     1021 caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag
     1081 atcctgctgc agattaaaga attgggtaag tgcttggctg atagcagtaa aaaaagaact
     1141 aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa
     1201 aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaacacaga
     1261 aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc
     1321 gttgattcct agtaagcctg aatctgctaa aggatttctt gtcactcctt gcatcaatgc
     1381 accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat
     1441 atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa
     1501 atctgctttt cccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag
     1561 aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc
     1621 tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg
     1681 acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca
     1741 ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt
     1801 gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt
     1861 tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca
     1921 tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt
     1981 tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc
//
LOCUS       DANKHJ010000237         2026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000237 DANKHJ010000000
VERSION     DANKHJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            89..754
                     /locus_tag="PW113_003393"
     CDS             89..754
                     /locus_tag="PW113_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527620.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT          705 a          368 c          320 g          633 t
ORIGIN      
        1 taccgttttt catcaaaaaa acgtatctat caagtgtata ggtcatgtag aataaatcta
       61 tatactgtag aatcgaaagg agaagaagat gaacaccgga gaaaaaatac gaacttaccg
      121 cagaaattgt aagcttacgc aaaaagaatt aggcgaaaaa attggtgttt ccgataaaac
      181 gatttctagt tgggaaaatt ctcgtacaat gccagacctt gaaatgctgt cacttttaca
      241 tcatacttta ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga
      301 accaacattg ccagatgaac ctcttgttaa caagaaatct tattcatcaa aacaaccctt
      361 ctcactcaaa aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct
      421 tatcaattat caatacaaaa atgaaacgat cgatcgattc aatccttttt tgaaaccaca
      481 aatcggctat accacactcc ctgtaggata cgtggacaat tccatcgaac gttgggtatg
      541 taatgatatc tttggccatg gtacatggct gtcttttcac gctggagagg tagaaaaaga
      601 gaaaaaccat gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac
      661 ttgggaagcc attccctcag attaccaaga aatcctcagc caagactatg acgacgcctt
      721 aaaaagaagc cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa
      781 aaatcaatgc acatgatctt atccaaaaaa caggggtaca aattaattga aaaagccttg
      841 agatgaaagt tcctaaataa aaaagttcag aatgataggt atttctggct tatagtataa
      901 tatttacgcc acagcctccc aatgattctt tttggaatca taatcaagga tggacattca
      961 caaaaatatt ttgctcattc gatccgaata gattaacgac caatctagta agtaatatag
     1021 attggtacgt agtcaagctg cacataattt agatcaattt tagttctttt ttatatttct
     1081 atgatctgct catttacttt tatatgatgg cttacagtaa cataaataaa tatgataatc
     1141 gctggtagtt tatccaagag tactcaataa aatacaaaat ggattgttca agcgcctatc
     1201 ataatgataa aatgaaacaa ataaacgata caaggaggaa gagactttga gtaatggcag
     1261 aacaattttt tcattgtaaa aattgttcag ttgttaatct atgggatttc ggtagcatgg
     1321 gcattttttt atagaggaat cacaccaacc tggccgtttt atctcttgtt agtgggaata
     1381 ttagtaggaa attttgcccc tcttcactat cgtacgaact tttttccaac tgattctggc
     1441 ctttttctta aaacaaagac gcgaaaatta gaaaacatta ttgaaatgac aattacctgt
     1501 ataatcattt tcttagttat attcctaaac gtcttctaat aagaatctct taaaattagc
     1561 ttgttacagt tagttaccta gtttatataa cacagttatc ttatcttcct tatcaacatc
     1621 ttttgccaac tctttgtctt aaattcgaaa catataagca gagctacgaa caccaagact
     1681 gtttcggctc tgttttttgc atcaaattca ctcttatata gctgataatc tcggctcttt
     1741 aaaagaaatt attttcgctt cattttatta taaaaaggct aggaaccttg atgttatcag
     1801 gttcctagcc tttatttaag tattacccca cagacccttc catatagatt tttaaattac
     1861 acactagttt aaactaaaac aaacaaggaa acacttacca tttaatcata aaattagctc
     1921 atatgaatat attagaatgt aaaatagact ggtcaaaatc tggtcaaaaa agaggactaa
     1981 tacaggtagt cctcagactg tagacaaagt ccattttatg gatgat
//
LOCUS       DANKHJ010000238         2011 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000238 DANKHJ010000000
VERSION     DANKHJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(97..639)
                     /locus_tag="PW113_003394"
     CDS             complement(97..639)
                     /locus_tag="PW113_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527621.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
     gene            complement(952..1503)
                     /locus_tag="PW113_003395"
     CDS             complement(952..1503)
                     /locus_tag="PW113_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2527622.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            complement(1796..2011)
                     /locus_tag="PW113_003396"
                     /pseudo
     CDS             complement(1796..2011)
                     /locus_tag="PW113_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
BASE COUNT          609 a          386 c          354 g          662 t
ORIGIN      
        1 ccacatgaat gatttgaaat cgtcttaaaa tgctttattt tataacccaa aatagaataa
       61 ttacttcaaa atgacgtcgc atatcactat taatgattaa tcgatgactt ccataatcat
      121 tttaagttcc ccggatgtta aatcctcgct aaaaatgcca gtaacttttt gtccaggtcc
      181 aatttccttt gatgataggt ttaacaaaga gatagggata tagattaatc cgttttgttt
      241 caaagacaga gccaatattt ttttccttgt tcgaatagct gcatcatagc gaagagaaga
      301 gtaaacacct ctgccaacaa tagaaatatg cttccccaat aataaatcaa ggctacctat
      361 tacttgtaat ttctgaaagg tcgtcccatc cgttttgatt tgaatatcct gtaaatttat
      421 tttcctgtct aaggcatctt gaaaaaacaa gtgtgtgcct ctgcgataat caatgccaca
      481 gaggtgcata aaattagttg tcgaaaaaat aatcgctacc gttgagactt ctccgacaaa
      541 ggatactttt ttatctacaa aatgttcttg taagagatga gcagccttat aaatcgcgtt
      601 taactgtctg gtaatttggg ttgtctcttt tatagacatg cgtttgtatt ctttttttgc
      661 cgcgtgcgaa gaatgctcct ttttcccaat aaaaaaagct gctcaaaaga gcagcctttt
      721 gtacaggttt agttttggtg cttgccaccc gccggaccct tacgttccgg atttgtatcg
      781 agtttagttt tggtgctcgc cacccgccgg acccttatgt tccggattta tatcaagttg
      841 actatgagcc tgctctcatt tgtatatata atataccaaa aaaaggttga tttgtccaat
      901 actgtgggtg attataaccg tcttactgtg cttatctatt atataataca atcaaaaacc
      961 taaccgaaaa tttaataacg tttcactgat ttcatcttca ttaattccga tgtagcgttt
     1021 ggtaatcttc tcgctgctgt gaccaaagat ttccattaag gtggccacgt ctttggtttt
     1081 cttgtaatag tggtaaccaa aggtttttcg tagggtgtgg gtgccgatat cgtcccttcc
     1141 taacagcttc gcgacctttt gaaacatctg gtagaccgta ttgacttcta aatggccgcc
     1201 cttagtactg ggaaacaagt aagcctccgg atctaagggt gcggtatagc cgtgaatcaa
     1261 gtcctgtaag ctgcttaagt ataaaatgcg tgttttccct gtcttttgct ccacgatgcg
     1321 cgggtttttt gaggtaatca cgtctttttt cttgagttta acgatgtcgg acatgcgcag
     1381 accgctgtta attccaatta gaaacaagaa aacgtcacgt tccgcatttt tattccgtcg
     1441 caggcaaaat aaaaaatcat taatctcctg ttgcgaccgt agtggctgaa cattatagcc
     1501 catgacaatc cctccttttt agtctgttcg aatacatgat tatagttatt acaacacgaa
     1561 atcgtgtgtt tagggggagt aaaaaaaaag aaccctataa ggataggatt cttgatacat
     1621 gattttatcc aatttcatgt atcttatatt caacccgcgc cacaagagta tggttgatat
     1681 atcattttga ctttgaacat ctagataaat ataaacaagt aatagagata aaaaatgtag
     1741 tgtaatcctt aattatctct attactcgat taatttgaag ttgtgcaatc attctttaga
     1801 ctgcctttag caatcgaagt ccattcaaaa taacaaccaa agtacttcct tcatgaataa
     1861 tgacactgat tgcaatatcc gttaagccta agaaactaac gacaattaaa aaagcaacaa
     1921 cggccattga aaaaataata ttctgtcaaa taacacggtt cattttcgaa gaaatattat
     1981 gagattgtac caatttagat aaattgtttt g
//
LOCUS       DANKHJ010000239         2001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000239 DANKHJ010000000
VERSION     DANKHJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..376
                     /locus_tag="PW113_003397"
     CDS             <1..376
                     /locus_tag="PW113_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2527623.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /gene="yfcC"
                     /locus_tag="PW113_003398"
     CDS             424..1824
                     /gene="yfcC"
                     /locus_tag="PW113_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2527624.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1857..>2001
                     /locus_tag="PW113_003399"
     CDS             1857..>2001
                     /locus_tag="PW113_003399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527625.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DANKHJ010000240         1986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000240 DANKHJ010000000
VERSION     DANKHJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1151)
                     /gene="cls"
                     /locus_tag="PW113_003400"
     CDS             complement(<1..1151)
                     /gene="cls"
                     /locus_tag="PW113_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2527626.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAEL"
     gene            complement(1303..>1986)
                     /locus_tag="PW113_003401"
     CDS             complement(1303..>1986)
                     /locus_tag="PW113_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527627.1"
                     /translation="TKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQNEMGDSWKL
                     FEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIFQIAGAVRTW
                     GLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFFLEGGWIGLI
                     GAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIGVIIGSLGSI
                     ISMRRFLKF"
BASE COUNT          624 a          423 c          334 g          605 t
ORIGIN      
        1 agctctgcag cgaaagaata cgttgcccag taaacgagaa gatgatctgg tttgttaggt
       61 atttggatat gaacttttac gcctgaaaga agagccaact tcagtgcttc atgaatcgat
      121 ccatccggta tgtaataagg cgtttggatc aatatctccc gtttggctaa cgaaatcatt
      181 ttcaaataag tcattttgat ttgttcatgt tcggtatctg gtccgcttgt cacgatttga
      241 acagctatat caccagtaga gtgaatggac ggaaaaaatt tgggagaata gcctacttct
      301 tttgtatgct gagaattcca gtccatcaaa aaacggtttt gcaaggtata caccgcttct
      361 ccgtaaatac gcaaatgatt gtctcgccaa taaccaaatt tctttaccaa tcctagatat
      421 tcatttccaa cattgaaccc acctgtatat ccgattttcc catcaataac tacgatttta
      481 cgatgattgc gataattgat ccgaggatta atatacggaa caaatagagg aaagaaaaat
      541 gcgacttctc cgcctgcttt tttcaaattc tgaaaaaagc tggaagaaac ttgtgtggaa
      601 ccccatgcat caagaaggac tctgactttt acccctcgtc ttgccgcctc tgtcaatgca
      661 tctcgaactt cttcacctaa tgcatcactg cgataaatat aatactggaa gtggatatgg
      721 tcttctgcct gcttgatatc ttcaatcagc gcatcgaatt tttcacgacc atctgtaaat
      781 aaagtgattt catttttagt cgtatagagc gtactttcaa ataccgtgag catatagatc
      841 aattgtttca catctacttg tccagtaggc ggatgaggat aaagccctcg catcaatgcc
      901 tgtttgtctt cttcaatttc caggttctta ccgattttcc cttggatctt caaatcaaag
      961 attttctctt tggaaatccc tcttcctaaa aaaatatata aaataaatcc tacaactgga
     1021 atgaacatca aaacgagcac ccatgcccat gtttgagcag tttcctttct ttctcgaaaa
     1081 acgatgataa aggaaagcaa aatattcata atcaaaagga tcgtaaaaaa attgtcaacg
     1141 ataattttca cggcttcacc tcttcttcta ccaacttaag aatacccaag ttgtttttta
     1201 aagtaaaggg aaaaaagccg cttccttcta ttctcctagc caataaaaaa agaaagctgg
     1261 aacgaaactg tccaactttc tcattttatt tatattgtgg tattagaatt tcaagaaacg
     1321 tctcatagaa atgatcgagc caagtgaacc aatgatcaca ccaatgccga ccattaaaag
     1381 attgattttc caaacgatat tttgtggatg aatcaatgaa taatgggaac gtaataatga
     1441 tggattgatc aattggaaaa accatgcata accaaatgtg atgatcaaaa tcggaagaat
     1501 cgcaccgatc agaccgatcc agccaccttc aaggaaaaac ggccagcgaa tatatccatt
     1561 ttttgcaccg actaaacgca tgatttgaat ttcacgctgt ctagacaaaa tcgtgatccg
     1621 aatcgtgttc gaaatgagaa acatcgctac gaacagcagc aatgccgctg caactaatcc
     1681 ccatgtccga actgcaccag cgatttggaa aatccgatct gaggaagctc caccgtaatc
     1741 agaacgagaa acattcttca aatctgctgc ttcttctgct acttgttttg tataagaagg
     1801 atcagtagca cttactacat acacatcata taacgggtta ttgtcacctt caaacaattt
     1861 ccaggaatca cccatttcgt tttggatttt tttcaattcc tgttctttgc ttgaaaattc
     1921 aactttttta acatgatcta agtcattcaa ttctttttct agctttttca tctcttcgtc
     1981 ttttgt
//
LOCUS       DANKHJ010000241         1942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000241 DANKHJ010000000
VERSION     DANKHJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(254..751)
                     /locus_tag="PW113_003402"
     CDS             complement(254..751)
                     /locus_tag="PW113_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2527628.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(881..1591)
                     /gene="budA"
                     /locus_tag="PW113_003403"
     CDS             complement(881..1591)
                     /gene="budA"
                     /locus_tag="PW113_003403"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2527629.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1604..>1942)
                     /locus_tag="PW113_003404"
     CDS             complement(1604..>1942)
                     /locus_tag="PW113_003404"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="catalyzes the formation of 2-acetolactate from
                     pyruvate in stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2527630.1"
                     /translation="GGFLFSAQDLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRA
                     SGVDFGPVDFVKYAEAFGAKGIRATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKL
                     GETILPDQFY"
BASE COUNT          558 a          411 c          291 g          682 t
ORIGIN      
        1 cactttttgt ttcaagccaa taggttggca aatatatttt tttcctaaaa tgatagtttc
       61 gttattcata tttcatccat ccttaatgtt ttttcattat ttaaaatgat aataacatta
      121 aaatatgatt atgaaaatca ataacacttg tgattttata aaaaaataaa aaaaccgaaa
      181 aacagaaatg cccactgtct ttccggctct ttttattctt gatttctctt cttttttgtt
      241 tcaatagacg attttagtta ttaataaatg aaaaagcatt tatattaaaa tttgctaata
      301 aaagatctac atatttttct agaaatgctt gatcgatttc atcataccct ttcgttactc
      361 cgcaatcgat atctaaaaca ccgattaaat taccttcttt taccattggc actacgattt
      421 ctgattgtgc ggctgaatcg caggaaatgt aatttgcatg ggttttgaca tcatcaacga
      481 tcaatgtttc ttgtttttca gctgattctc cacaaactcc ttttcccatc ttgatacgtg
      541 tgcaagaaac tcgcccttgg aacggtccta aaatcaattc attaccatcg aataaataat
      601 atcctgcaaa gacagtctca ggcaaagtct cattcaacaa tgcagatgaa tttgctaaat
      661 ttgctaacca gttatcttct gcttctaata atccttgttg ctgtaataat aaaatttcgt
      721 atgcatcttt tttctgtttt tctgtccaca tgataaaaac cgctcctttt tgatctatgg
      781 agaagattat agctcagttt ttttgtaaac gctaggtttt ctcctcaaaa aaacgagata
      841 cgtcaggtta atcaccttga acgcatctcg ctgtcttttt ctattctgct tgttggattt
      901 ctgctgctaa atcagcataa tcaatcgttt cgtgaacaaa agtcggatca tcaactggaa
      961 aatgctgtac aagggacgag atgcggctaa tctcaacttt tccttcagcc acttcaaaat
     1021 ccattacatg tccgccaaat gtatgtgtat catcgataaa atgcaggtga aatcccgctg
     1081 cagcaactcc ttggaataat tcaggcgtaa agaacccgac taatgttcct tggacattgc
     1141 tttgttggaa ttctggttga gactctgaaa tttttgctag tcttctgtaa ggtttttctt
     1201 gcttcggcat gatacggatg tgcatatttt tgaataatcc agttatcttc actgccaaaa
     1261 ataggttttc cccggcttct cttactaata tgtctttttt caattgttct ccatctgcag
     1321 tttttgagac agaaaaacta gtatctggcg taaaatctgt aattgctgca tagggggtaa
     1381 gttcacttcc atctaattgg gcaactgagc catctgatct tccttgataa gctacgccat
     1441 ctaaaaagat gacttcacca tcaagcccat gcaatgttcc caatcccaga gttccttttt
     1501 ctaatatatg agcaatcgtt tctgttccat ccatcaagcc ggccatcaaa gctcctaatg
     1561 tcccgtgctg gtataagatt ttttcagtca tgtcgacact tccttaatag aattgatctg
     1621 gtaagattgt ttctcccaat ttctcattat cacggtaatc aatcggaata tcaataatta
     1681 cgggtccttc tgtcgcaaat ccttcttcta aagctttttc taattcctct acacttgtag
     1741 ctcggattcc tttagcgcca aaagcttctg catatttcac aaaatcaact ggaccaaagt
     1801 ctacacctga cgcccgctga tatttcattt tttcttggaa ctctaccata ttgtagtgtc
     1861 catcattcca aatcaaatga acgatattca attttttacg taccgccgtt tctaaatcct
     1921 gggcagagaa taaaaatccc cc
//
LOCUS       DANKHJ010000242         1913 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000242 DANKHJ010000000
VERSION     DANKHJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..674
                     /locus_tag="PW113_003405"
     CDS             <1..674
                     /locus_tag="PW113_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527631.1"
                     /translation="GLLHTKLKIPALLAGIITMTGLYSVTSRVMGAPNISLLGQPTIF
                     HWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETGLAIRATGDNLAMSEANGINT
                     DNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTIVIGLASIIIAEVLLRNQPLF
                     LRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVLVICLSFPQIQQKFHLPQKKK
                     GGSVK"
     gene            671..1477
                     /locus_tag="PW113_003406"
     CDS             671..1477
                     /locus_tag="PW113_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2527632.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            1613..>1913
                     /locus_tag="PW113_003407"
     CDS             1613..>1913
                     /locus_tag="PW113_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2527633.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKD"
BASE COUNT          544 a          405 c          406 g          558 t
ORIGIN      
        1 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
       61 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
      121 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
      181 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
      241 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
      301 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
      361 ctggctcctt gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
      421 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
      481 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
      541 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
      601 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
      661 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
      721 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
      781 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
      841 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
      901 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
      961 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
     1021 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
     1081 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
     1141 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
     1201 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
     1261 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
     1321 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
     1381 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
     1441 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
     1501 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
     1561 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
     1621 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
     1681 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
     1741 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
     1801 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
     1861 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atg
//
LOCUS       DANKHJ010000243         1912 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000243 DANKHJ010000000
VERSION     DANKHJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>1912
                     /locus_tag="PW113_003408"
     CDS             <1..>1912
                     /locus_tag="PW113_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527634.1"
                     /translation="SDDGVAWSLIKQGVTDLAYINKTDQYSSIDKTEDRNNLTRKNFQ
                     QTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDNFLSTFSDSLKSG
                     YYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLVFATDIETGQRSK
                     VVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIAYICAVFVLIKGS
                     GTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGEGEDRKPEKNFGE
                     TLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDIANDTKNKFKEGL
                     DDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVENADRIMSSSERMR
                     EAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQKRHEAKYTPEAMN
                     REEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKENPQNSTSSHGGRS
                     VDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSSSTSQPKTVDVRE
                     NVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVDVRENIQKD
                     SGEMKEKTQKINI"
BASE COUNT          719 a          280 c          412 g          501 t
ORIGIN      
        1 tcagatgacg gagtcgcttg gtcattgata aaacaaggag taacagattt agcatatatt
       61 aataaaacag atcagtactc aagcattgat aaaacggagg atcgaaataa tttaacacga
      121 aaaaattttc aacagacgga tctcacacaa gtgttaacgg ataaagtgat tgataaattg
      181 gaaaaagaaa atccaattgc ggacaatctt cgttatgaat tagttgaaaa ttctaataat
      241 gaatttgttg ctacaaagtt ttctgacaac ttcttatcaa cattttctga tagcttgaaa
      301 tcaggctatt accgatacca agcaaatcta tgggggattt caattgggtt gacagcgtta
      361 gcaatcgcct atgtgttcag cgcgtttgtg atcattacag caatactaga acttgcattt
      421 aaacgtgtgt taggtgttct tgtttttgca acagatattg agacaggaca gcgttcaaaa
      481 gttgttctat cagatattct acaatgttac ttaactgttg ggtttcaagg atttgggtta
      541 tcaatgtttg ctatgtttat caattttctg aactcaggac aaggtatctc aacaaatatt
      601 tttataaaaa caattgctta tatctgtgcc gtttttgttt taatcaaagg cagtggaaca
      661 gtcatgagat actttggtgt tgatattgga ctcaaagaag gctatgggca acttgcttct
      721 gcttttggaa tgggcgcaat gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa
      781 ggaagcggag aaggagaaga tcgaaaacca gaaaagaatt tcggcgaaac tctatcaaaa
      841 aaagctggca aaactggaag agctttggga tatgcccatg aacgtggatt atccggttta
      901 gcttcagatg gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt
      961 gatatagcta atgataccaa gaataagttt aaagaaggtt tagatgatgg tacagtgtcc
     1021 gctattaata aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa
     1081 tatgctgatt ccgtgccaac acgtctttca gatagaaaag atggtgctaa agttgaaaat
     1141 gccgatagaa ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa
     1201 tcgaataata atcctgcaag cgcaattcag caaaaagtcc aacaagatat tgaagaacgt
     1261 aaaaatgcta tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgtcat
     1321 gaagcgaaat acacgcctga agcaatgaat cgcgaggaaa taatgttaag gaaacgtgta
     1381 gaagggcgga caggcgctaa tattgacgga aaagaagcgt tgaccaaaga aagaattcaa
     1441 gaagctaaaa attcaaatac aggattagaa aaattagtca aagaaaatcc acaaaattct
     1501 acaagtagtc atggtggccg atcagtagat gtccgagaaa atattcaagg ttcactgaat
     1561 ggtcatgttg gacggaatgt tgatgtaaga gagaatttac aaagttcttc aaatgctcaa
     1621 ggtactaaga caattgatgt tcgagaaaat ctgcaaagta gcagtacaag tcaaccaaaa
     1681 acagtagatg ttcgagaaaa tgtacaaaat tctacaagta gtcaagggtt taaatcagtg
     1741 gatgtccgag agaatgtgca aggtagcagt gcaagtcaac caaaaacagt agatgtccga
     1801 gaaaatgtac aaagttctac aagtagtcaa ggattcaaaa cagtagatgt tcgggagaac
     1861 attcaaaaag atagtggaga aatgaaagaa aaaacacaaa aaatcaatat cg
//
LOCUS       DANKHJ010000244         1911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000244 DANKHJ010000000
VERSION     DANKHJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..969
                     /locus_tag="PW113_003409"
     CDS             <1..969
                     /locus_tag="PW113_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2527635.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="PW113_003410"
     tRNA            1159..1232
                     /locus_tag="PW113_003410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          639 a          327 c          362 g          583 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DANKHJ010000245         1886 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000245 DANKHJ010000000
VERSION     DANKHJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..122)
                     /locus_tag="PW113_003411"
     CDS             complement(<1..122)
                     /locus_tag="PW113_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2527636.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSS"
     gene            complement(133..330)
                     /locus_tag="PW113_003412"
     CDS             complement(133..330)
                     /locus_tag="PW113_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2527637.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(353..646)
                     /locus_tag="PW113_003413"
     CDS             complement(353..646)
                     /locus_tag="PW113_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527638.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            complement(683..820)
                     /locus_tag="PW113_003414"
     CDS             complement(683..820)
                     /locus_tag="PW113_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2527639.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(822..1268)
                     /locus_tag="PW113_003415"
     CDS             complement(822..1268)
                     /locus_tag="PW113_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527640.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1265..1414)
                     /locus_tag="PW113_003416"
     CDS             complement(1265..1414)
                     /locus_tag="PW113_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527641.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            complement(1431..>1886)
                     /locus_tag="PW113_003417"
     CDS             complement(1431..>1886)
                     /locus_tag="PW113_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527642.1"
                     /translation="DGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKID
                     ERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRP
                     PISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
BASE COUNT          510 a          394 c          296 g          686 t
ORIGIN      
        1 gaactacctt cgtttacacc taaattaaat tcattgttaa tcgtataggc aaacccatta
       61 atcggcaata aaaaaagagc cgttaatagg ctcatcgcag taatagtaat tttctttttc
      121 atttgtttcc tcctattttt tcaaattata agccgacaca ccagtgataa cacctaaaaa
      181 tgttgctgct gcattgatag tcagaactgc catatctgtt ccattccatc catatgcttt
      241 gcctaatgtg gctactaata cagataaagc tggcaatact gttaaaactg tccatttaat
      301 gacttgataa tacttatcgg gtaaaatcat ttcttcttaa ctcctttctt ttttaccgag
      361 acctttctct aaataaagtt ttaatttgtt gtgtgtgttc taccaatttt tctgcatgtg
      421 tatctaatct ttcatcgtgt ttctttagtt cttcatgaat catcaatcga tctgatttgc
      481 tcgattctaa atctttagtc agcaaatcta aattgtgact tacttttgaa agagtttcag
      541 taatcttcga gaaagatgca gtgactggtc ttattactaa taaaatcaaa gaaacaatcg
      601 cagtaattga tcctgcgatt gttccccatt cccctaaatt gatcatgtga caactccttt
      661 accttaaata aaaacgcatc acttaagatg cgctttcttc tttgctaatg attttatctg
      721 cttcttcgtc tgtaatgcat agtggaacga atagtcgaac ttgatcgtca gtaaaacagc
      781 cccaatcata catcattttc acatcgctaa aactaaacat actactcacc tccctttgaa
      841 gctggattta gttgctcttt aatttctgaa atgtctttgc tattttgtaa cgaagcaagc
      901 atcatttttg aattgatttg tgctaaacta tccgcttttt ctttcaatgc agtattttcc
      961 tgtttaattg ctacatcgct tagcatgagt ttggcattga tctgttttaa atcgccgttc
     1021 tcattttcta acgactcata cattgctttg agattgttta aatcgttgtg atctagtgcg
     1081 ttcgctaaaa taatccattg attcaattta ggatcaaaca tttgatcagc gattgttaac
     1141 ggttcgccat cagcacgaat tccttcaagc ggtggctgat ctgtgtaagg aacggataca
     1201 agcatgtcgt ccaatacttt tcctgcgtac tctccgccag tacgtccata tttccaaatg
     1261 tttttcattt atttccctcc cagtaattga atttcggttt ccaatttaga atcggtggtt
     1321 cgacttctgt gcattcttcc ggtaaatgtt cttcatcatt cacaatgatt tgctcgaatc
     1381 cgtaaggttc aattggtcta tatgctgcct tcatatcgat tcacgctttt ctaaatcgtg
     1441 taagtaattg caaaggtata atccgatcca taacttgagt ttcttcgcca tttaatggct
     1501 ccatctgcac caatagataa ctgagcactg ttcaaagtag aacggtctat cgagccaacc
     1561 agttgctcaa aactaattgg tggccgatag ccttctggaa ttgttagtat cgttgaatca
     1621 tttccaccac tgctttttcc gtttaaagcc acaaaatata tagaaactgt ttttccttca
     1681 cgataaagct ttgctgatcc ggtattcccg tttgtaactg ttaatgtggt agtagctgta
     1741 tcattaatgc gttcatcgat cttattgtct aattcatcta tagcagtcgc attagcattc
     1801 gcttttgttt gagcatcctt agctgtggtg tctacttcat taattgaagc agtcaactgc
     1861 gaattaatct ccgatacttt cccatc
//
LOCUS       DANKHJ010000246         1872 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000246 DANKHJ010000000
VERSION     DANKHJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..537
                     /locus_tag="PW113_003418"
     CDS             <1..537
                     /locus_tag="PW113_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527643.1"
                     /translation="NTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFE
                     GGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSG
                     DFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKT
                     MKNILKNVKIAESLDDIL"
     gene            534..>1872
                     /locus_tag="PW113_003419"
     CDS             534..>1872
                     /locus_tag="PW113_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2527644.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILII"
BASE COUNT          659 a          221 c          331 g          661 t
ORIGIN      
        1 aatacaatac aaattccatt agttacagct gggtcaagaa ccgcattgca actttcggat
       61 atttttaacc ctatgataga tatttataaa gaaaaaaaat atatattttt tacgtctgtt
      121 tatgattttg agggcggaga acctgtcgga gaatatgaat tagtatgcaa aatagcagac
      181 ttagtaggaa aagataatct aattgttaag acgcatccta gagatgtaag aacaatttat
      241 aaagataatg gatttatcga ggataaaaat tcatcaatac cttgggaggc gattcaattg
      301 tcaggcgatt ttaacgataa aatatttatg acaattaatt caggaagtgt tatttcaggt
      361 agtacaatgt ctgcaaaacc tgtacgcacg tattatatgt ataaattgtg taacatagaa
      421 ggaaatccgt cttgcaaaaa gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg
      481 atgaaaaaca ttttaaagaa tgtgaaaata gcagaaagct tagatgatat actatgagca
      541 aaactaaata tttaattaaa aatatgggaa tattaacaat aagtaatttt tcttcaaaaa
      601 ttttagtttt tttgttagtc cctttatata caagtgtatt atccacaaca gaatatggca
      661 catatgattt gacaatttca acagccacat tattgtttcc aattttaacc ttaaacattg
      721 tagatgctgt gatgcgcttt ttaatggata aagataaaaa taaaaaaaat atagcatcta
      781 tagcgattaa agttatattt ttaagcgtga tagtctttgg atccgtaatg tatttgttaa
      841 atattacaaa attatggcaa gatgtttatg gattagaaaa atatattttt ttgtattata
      901 tatcctatgt atggaatcaa tttctaatac aatttgctaa agggttagaa aaagttaaag
      961 ctatgggcat agcaggtatt atcagcacaa ttgtaacaat aattgccaat attttcttct
     1021 tgcttattct gaaatgggga ttaattggtt ttttcattgc aaatatttta tcacagatta
     1081 tttcagctac ttatctgtca gtattagtga gaatatggga atatatcgat tttgaggcta
     1141 aagatcctca gctaaagaga gaaatgctaa attactcgat tccattaatt gcaactgttg
     1201 ttggatggtg gattaatagt acagctgata ggtatgtggt ttcctttata gtaggtgtct
     1261 cggcgaatgg cttattgtct gtggcatata aaatacctca aataattaat actttgcagg
     1321 gaatatttat acaggcctgg caaatttctg caattaaaga atatggagat aatgagactg
     1381 caaaatttta tggaaataca tttgttatga ttaatttgtt aatgtgcata acctgttcaa
     1441 gtcttatcat attaactaga ccgttttcac atattcttta tgcgaaagac ttttatgaag
     1501 cgtggaaata tgttccattt ttattaatag ctaatgtttt aaattgtgca tcaggtctgt
     1561 taggacctat cttatcagca aaaaaagatt ctaaatccat gatgtggtct gcaatcatag
     1621 gggcattagt taatgtgatt atgagtgtag tccttgttta taagataggg gttcaaggtg
     1681 ccacaatagc cactgttata tgttcatatg taatatatgt tgttcgtaaa cgagctgtgg
     1741 gaaaagatat ttttataaag cattatcata ttgttgtgga atcttgggga ttattgtttg
     1801 ttcaggcaat catagaaata tattttaaaa attatgtaat agaaattata attatgatta
     1861 ttttaattat ta
//
LOCUS       DANKHJ010000247         1859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000247 DANKHJ010000000
VERSION     DANKHJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="PW113_003420"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="PW113_003420"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2527645.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DANKHJ010000248         1858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000248 DANKHJ010000000
VERSION     DANKHJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            71..385
                     /locus_tag="PW113_003421"
     CDS             71..385
                     /locus_tag="PW113_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527646.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            489..1667
                     /locus_tag="PW113_003422"
     CDS             489..1667
                     /locus_tag="PW113_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527647.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          631 a          272 c          385 g          570 t
ORIGIN      
        1 gtactggagt gtttttttac aattttaagg ggtaccgtgg tggtcgtttt gattttgaga
       61 gagaagggtg atggagttgg aagttaaaac aaatgttaat tctctggaaa tagagttcta
      121 ttcaatttta caaaaaaaac tggaagatta cacggaaaaa ttgcttgcag tcagtcattt
      181 acctctggta ttaacaaata ctgatttaaa gcgactattt cagataagtg atagtacact
      241 gaaccgttta gtaaaacttg gtgagtttcc tccctgttgg tacgggattc gaggacacta
      301 ttttcgtgat gatgttttag aatggatgag aaagagtgat tcgaatgact atcgagaaaa
      361 attgcgacta cttcgatctt tataagttac attttctttc taagatgcgg tacaatgatg
      421 acatagatag aaagatttcc aaagggtttc tgcttgttgg gttccttcaa aataggagga
      481 atcaataaat ggaaaaaaca gatcgattca agaagcaaat cagttctact ggtaggatca
      541 catggtactt tcaagcatat cgaacaacaa gacgtggctt taattcaaaa cgtgaggcgc
      601 aactcgctta tctggaaatg gagaaacaac aaaggcttaa gaaaaaaatt gttgcaagca
      661 atgacaagtt tagtgttgta ggtgagcaat ggttccaata ttaccgttcg ttaaatgaac
      721 aaaaggaaat aacctatgat aagcgagagg aacaattaaa aattattgtt cgatggattg
      781 gtgataaaaa gttgtctgat ttgacaccag agtgccttca agaattgctt tttaccttaa
      841 aggaatgtgg cgttaatgga acaaatgttg gctatgccaa aaatagttta caatcactta
      901 ttcaagttct aaatatgatt tttaaatatt gtcaaagaaa gaatttgata agtgaaaatc
      961 caatgaagtc tgtgagaatg ccaaaatatc agttaacggt aactgattta aaagaagcca
     1021 taaataatat tgaggacaag tttcttactg tcgatgaatt gagaactttt ttgaattatg
     1081 gcattgttca tgaggaatta ccgatgtctg tacttttcca tgttctattt tacactggct
     1141 gccgagtcag cgaggcgctt gctctacagc ctggagatat agatttcaaa agaaatgaaa
     1201 ttctgttcta taagcaaacg gcagttaaag gtaagagtaa ggattttcga attgaaacta
     1261 ctaagacagt tagttctgct agacgagttc tagtaactcc tctagttatg gaaaagctac
     1321 aacagttgat taaggctttg ggtgagatga ggcaacatta caggtttatt atagaagaaa
     1381 catatttgtt tgtgtatctt gattctagta aacgtggtgt tccttatcgt cgagagtatg
     1441 taaatgatca cgtcaaacgg tgcgtgaatc gctcaggaat taataagcca tttcatactc
     1501 atttagctcg tcatacaatg gcaagcttag tagcggaaca ctgtagctgg gaagtcttga
     1561 aagatcgctt aggccatact gaccaaacaa cttcgaaaat ttatcgccac ttaacgtcaa
     1621 atgaaaaggt aaaacctctg attgctttta gttctttaga gagttaaaaa aacggtgaaa
     1681 gcactggtat tacaggattc ttgaaaaacg attttttaac aaattagtca atatgtagtc
     1741 agaggtaata aaaatccttt tatatcaata ctttaacgac ttttcacaag tatcaaagaa
     1801 atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca agggttct
//
LOCUS       DANKHJ010000249         1844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000249 DANKHJ010000000
VERSION     DANKHJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            469..1641
                     /locus_tag="PW113_003423"
     CDS             469..1641
                     /locus_tag="PW113_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL2527648.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          644 a          341 c          301 g          558 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
     1561 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
     1621 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
     1681 tggacttgac tcacttcctt tattattttt cattttttta aactatttaa tactaatgtc
     1741 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
     1801 ttttctacca ttttcgataa attcctgttt aatattttta attc
//
LOCUS       DANKHJ010000250         1844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000250 DANKHJ010000000
VERSION     DANKHJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(132..1511)
                     /gene="brnQ"
                     /locus_tag="PW113_003424"
     CDS             complement(132..1511)
                     /gene="brnQ"
                     /locus_tag="PW113_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2527649.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT          615 a          363 c          359 g          507 t
ORIGIN      
        1 tcattgtccg cagttttttt gtgctcgtaa aaaagagatc taaaaaatag ctagacgaaa
       61 gaataggatt ctttcatcta gctgtttttt tcctgtttaa tatttcttat gttaaattgt
      121 gtttgatctg gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga
      181 taggcgtaat agaagagtaa gctgccagca tacccaagta acgttgggac catccagcca
      241 agtcccaatg cagcaaaagg aacgtgatga tgataaaatt cgagcatgct tgctacaact
      301 ctttgttgac tcactaaagc aggcgaattt gccagcatat ccaatacggc gggaacagca
      361 gcaaacaaca tcgttacttg atagacaaat ggtgtctttt ggaaaaattt tgctgttaag
      421 gaaagcaaaa tcaacgcaag cgacaatgga tacaaaagca tcaatactgg taaagaccat
      481 tggatgatat tgtccaaacc agcatttgca acgacaaaag agacgaaagt cgttaaccgc
      541 agccatgtca tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca
      601 acgagtccca tagcggtagt gaagacacct aatgtgacca tcactcctaa gaagctagcg
      661 ccgaagttac ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat
      721 aaagacatct tattcaaact catcgcaccg agaagaacaa gaccaaaata gatcatgact
      781 tctagcgcaa cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatctttg
      841 aaaccaaggt cacggactgc atggacaaaa gtgacactta aggcaagcaa ggctactgca
      901 tcaaccgtgt tataaccttc taggacacca tttgttaaag cgtttgtttg gtaagtatct
      961 gcagcagatt gatttagatt tcccattggg tggaataaag aaacgacaaa aatgattgct
     1021 aataatgcaa gaaacaagga gtttagatac tttccgacgt atttgaccaa ccgtgtggga
     1081 tggacagtaa ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat
     1141 gaattatatt tttcaggcaa aaatggttta gcagccattt caaaagcagt agcagctgtc
     1201 cgtggtgtac cgaaaaatgg tccgatcgtc aaatgaacaa ggattaagaa caaggcagca
     1261 taccattttc cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca
     1321 gctaagatag ctagtaatgg gaaaagagcg cctgagatgg caaatcctac gccagcactg
     1381 acccagtttg cgccagccat ttgacctaag tggacaggaa agattagatt acctgacccg
     1441 aaaaacatcc cgaagataag tgaactgacc atcaatagtt gtttccatga ataagttttt
     1501 gtttcttcca taaatttttc ctccaataat tatcattttg attgacgata taaacgttgc
     1561 agcaattagt aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa
     1621 aaaagccctt ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc
     1681 gcttaaaaaa gcttcttcat tcacggacgc tttttgataa agttatccgc tagcctgata
     1741 atggaggcag ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat
     1801 tcgatttatt gtcatttctc acttacatga ctcactggga ataa
//
LOCUS       DANKHJ010000251         1823 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000251 DANKHJ010000000
VERSION     DANKHJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(70..141)
                     /locus_tag="PW113_003425"
     tRNA            complement(70..141)
                     /locus_tag="PW113_003425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Arg,seq:ccg)
     gene            201..1535
                     /gene="rpoN"
                     /locus_tag="PW113_003426"
     CDS             201..1535
                     /gene="rpoN"
                     /locus_tag="PW113_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2527650.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            1684..>1823
                     /locus_tag="PW113_003427"
     CDS             1684..>1823
                     /locus_tag="PW113_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HDL2527651.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RN"
BASE COUNT          644 a          310 c          380 g          489 t
ORIGIN      
        1 ttggcggaca gttttcactt aaaaataaag aagcaactat tcgtaaatta tgctaaaata
       61 gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca agaaccggaa tcttacgtga
      121 tatccactac actaaggacg caagcactgg gataattgta actaaacaga taaaaaatta
      181 caaggggatt ggacaaaaaa atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga
      241 cacagaaact agccatgacg caacagttgc aacagtccat ccaagtttta caatataaca
      301 gtgaggagct gttggcattc gttgagaatc aagcaatgga aaatccattg gtggaagttg
      361 ttgagccgga atggcagcca gattacataa aagcttcttc atcttcttat gaaggagaag
      421 agacaaatta cctaaatcag attcctgata caaaagggtc tttatttgat tcattgatcg
      481 aacaagtcca tttgaattac cgagatactt ttttgaggaa aatcgttctt tatcttgtag
      541 aatacatcga tttgaacgga tttttgacga ttagtttaga agaagccgga aaagagattg
      601 gagctacgcc tatccaaatg ctcgatgcgt tgactctgat tcagcaatta gaaccagctg
      661 gagtaggtgc acgtagtttg caagaatgtc tcatgcttca aacagaacga gatgattatg
      721 cgccggaatt agcttatatc gtgttggaag aatgttttga ggaactggtg gagcgaaaat
      781 ggaaagaaat cgcacaacgt ttttctgttg atctacatgc tgttcagcag atattcgact
      841 atttacaaac gttgagtcca agccctggcc ggattttcga ccgtagcagc gaattgtaca
      901 ttattcctga tgtccgggta ttagtggacg acgataaaaa tgtacaagtt atctcaaacc
      961 gaaaaaatca gccaaatata cgctttcaag agagttactt caaacaaatg tcacaacaag
     1021 cagataaaga aactgaaaat tatttaaaag aacggaaaca ggaattcgaa tggttgaaga
     1081 aaacgatcct acaacgtggg gatacaattt tgcgggttgc tcaagtcatt gtttcccggc
     1141 aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc tcttacgtta aaggaagttg
     1201 caactgagat agatgtgcat gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg
     1261 aaacagactt cggtgtattt gaattaaaaa aatttttcac tacacgaatt cctacgaata
     1321 gtacagaaca gacagaagac ctttcagcag atacagcgaa gaaaaagctg caggaattag
     1381 tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa attggttgaa ttgctgaaaa
     1441 aagatgagat agcgatctca agacgaacag tagcaaaata tcgtgattta ttaggaatcc
     1501 cgagttcatc aaaaagaaaa cgttatgata actaatcagt caaaagacta gaaaaagtat
     1561 ttcacttttt ctagtctttt tagttgaaaa acgagagcaa aactgttaga cttacagtcg
     1621 tgaggttgag atttcccttt attgatgtga aattcgaata gttaatttct ttttttatcg
     1681 tatatgggtc gttttaagtc tacattggac gtaaagtgtc caactaagga gatcgctatg
     1741 ctggatgaaa taaaaatgat tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa
     1801 cgctataaaa ttttacgaaa tat
//
LOCUS       DANKHJ010000252         1777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000252 DANKHJ010000000
VERSION     DANKHJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(95..1021)
                     /gene="trxB"
                     /locus_tag="PW113_003428"
     CDS             complement(95..1021)
                     /gene="trxB"
                     /locus_tag="PW113_003428"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2527652.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(1166..>1777)
                     /locus_tag="PW113_003429"
     CDS             complement(1166..>1777)
                     /locus_tag="PW113_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2527653.1"
                     /translation="AYALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTT
                     DNELLEQTAGNIFASVKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLG
                     AVDTPDENGYVEDDYRIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMK
                     KRYGFIYVDRDNEGNGTLARSKKKSFNWYKKVIDSNGENLSND"
BASE COUNT          503 a          417 c          293 g          564 t
ORIGIN      
        1 ggttgcaagt attcgcataa atcggattca gtagatttca cgcgaaaaaa aagagtaaaa
       61 gaacaattaa accgttcttc cactcttttt aaacttatgc agttgtttct tctaaagatt
      121 ccaagtattt atacacttgt tgtccagcga tcccaccatc accaacagca gtagtgattt
      181 gtcgcaaatc tttttcacga acatctccga tggcaaaaac gcctggtata ttcgtcttca
      241 tttcgttgtc tgttggaatc cagccagctt cgttcgtcaa ccctgcttct ctaaatggtg
      301 cagttaatgg atccagtcca acatagatga atactccgtc tgctgccaaa tcaaactcat
      361 tgccggtttt gacattacgt gctttcacac ctgtcactac catttcattc ccaacgattt
      421 cgtcaacgac tgtatcccac ataaaggaaa ttttttcatt cgcaaatgca cggtcttgga
      481 tgatcttttg tgcacgtaat tcatctctac ggtggacgat gactacttca gaagcaaatt
      541 gtgtcaaata gatggcttct tctactgctg agtctcctcc tccgatcact actagacgtt
      601 tgttacggaa aaacgcacca tcacaaactg cacagtaaga aacgccacgt ccagcaaatt
      661 cttcctcgcc aggtacgcct aatttacggt ggatacagcc tgttgcgata atgactgttt
      721 ttccttcgta agttttatct tcacaaataa cttctttgtg gctgccgtga tccttgatat
      781 cttgcacgat tccataagca ttttctgtcc caaatcttga aacaccttca tacatcttat
      841 aagctagatc aggacccatt attgaatcga aaccaggata gttttcgatt tctgccgtat
      901 tgttcatttg gccgccaggg gcgccttgtt cgatcatcaa tacggataag tttgaacgtg
      961 aagcatataa agcagcagtc atcccagcag gacctgcgcc aataacgatt acatcataca
     1021 tatgtagatt ctcctttact taatcaagta agcatacttt acagccaatg cgaaaaaaag
     1081 tctattgttc tgcttgcgtt tctcgaatag ctttattata ctcgaaaaat ccgctgaaaa
     1141 acaatagact tttataaatc attgatcaat cattcgaaag attttctcca tttgaatcaa
     1201 ttactttttt ataccagttg aatgatttct tcttcgaacg ggctaatgtg ccatttcctt
     1261 cgttgtcacg atcgacataa atgaatccgt accttttctt catctcacct gtaccagctg
     1321 atactaaatc aatacatccc caagtcgtat agcctaatag atcgactcca tctaattcta
     1381 ctgcatcttt cattgcctgg atatgtgctg ccagatagtc gattcgatag tcatcttcca
     1441 cataaccatt ctcatcgggc gtatcgactg cacctaagcc gttttctaca ataaatagtg
     1501 gtttttgata acgatcataa atatcattca tcgttattct caagcctaat ggatcaattt
     1561 gccaacccca ttcacttgct tccagatatg gatttttgac agaagcaaag atatttcctg
     1621 cagtctgttc caagagttcg ttgtcagtag tcgaaactcg agaagaataa taagaaaagg
     1681 agataaaatc aacagtatgt tctttcagca atgcggcatc cccaggttcc attttgatct
     1741 gaatgccatt tcgttccatt tctttcaatg cataagc
//
LOCUS       DANKHJ010000253         1773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000253 DANKHJ010000000
VERSION     DANKHJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            81..1604
                     /locus_tag="PW113_003430"
     CDS             81..1604
                     /locus_tag="PW113_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL2527654.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          284 c          359 g          489 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcatatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DANKHJ010000254         1765 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000254 DANKHJ010000000
VERSION     DANKHJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1394
                     /locus_tag="PW113_003431"
     CDS             <1..1394
                     /locus_tag="PW113_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2527655.1"
                     /translation="ITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQMDLAQAI
                     RARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITVLDVFVLL
                     LLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFSDSPVIGG
                     ETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDSNIQLSMA
                     FLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGILSTLFAV
                     ALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVLFTSTKGT
                     IEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKLLGWFSVI
                     SLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGDLYKGNKK
                     YEQRLQETIEEIE"
BASE COUNT          489 a          339 c          367 g          570 t
ORIGIN      
        1 cgatcactgg tggacaaaat ttccaatatt tattgatgtc agttattttg atgtctagtc
       61 tgattgctat gcttttacaa tatatggcag caaaattagg aatcgtgacc caaatggatt
      121 tggcccaagc aatccgagca cggacaagta agacattggg tgtcatttta tggatcttga
      181 ctgaattagc cattatggca acagacatcg cagaagtcat tggtgcagca atcgccctct
      241 acctgctttt tgatattcca ttggtaattg ccgtatttat cactgtgtta gatgttttcg
      301 tccttttatt gctgacaaaa gtcgggtttc gtaaaatcga agctttggtt gtctgcttga
      361 ttttcgtcat tttattcgtt ttcgtctatc aagttggctt gtctaaccct gattggcaca
      421 gtttgtttgc cggatttatc cctaacagtc acacattctc agactcaccg gtcatcggcg
      481 gcgaaactcc gctgactgga gctttaggga ttattggtgc aacagtcatg ccgcataact
      541 tatatcttca ttcagcagtt tctcaaactc ggaagatcga tcaccaagac gaagaagatg
      601 tagcacaagc ggtacgcttt tctacttggg attctaatat ccagttgtca atggcttttc
      661 tagtcaatgc actgctattg atcatgggtg tggctgtatt caaaaccggt tctgtccaag
      721 atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta
      781 tcttgattgc tgtagcaaaa tctggtatct tgtctacttt atttgctgta gcacttcttg
      841 cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggct
      901 tcatccatat gcgtatccca atttggctaa gaagactagt cacacgattg ctttctgtga
      961 ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg
     1021 ctttgaataa cttgatgaat gaatcccaag tttttctggc ttttgcattg ccattctcga
     1081 tgattccttt actgatgatg accaatagcc aagcggagat ggggagccga ttcaaaaatt
     1141 catttgttgt aaaactactt ggctggttct ctgtgattag tctgacttac ttgaatttac
     1201 gaggattacc aggtcaaatc gaaagtttct tcggcgagca agccacctca gctcaactag
     1261 cattggctga tcaaatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga
     1321 cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcgtttgcag gaaacgatag
     1381 aagaaatcga gtagaaactg attcttttac acaaaggagg ttgtgtcaga agtgggcagc
     1441 ttcaagaaat aagaaggaat ttaagaaaat tgcttttcaa atttttgtga aatttcggct
     1501 tatttccgaa gaagttactt ctggaacacc gtgtatcaga ggttgtgaaa aagctgcctt
     1561 gcatcaagta ataagaacag ccaagtaaaa acagcttttt atgtttttaa cttggttgga
     1621 cttattaccg cagatgcaag cttttgagca ccgtttattc ggaaaaagga actgcgacaa
     1681 gacttttgtc acagttccct ttcctacgat tagtcaagta aaaggtgtaa gaaaaatagc
     1741 tcataaacga aagcgacaaa atcaa
//
LOCUS       DANKHJ010000255         1713 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000255 DANKHJ010000000
VERSION     DANKHJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>1713
                     /gene="fss3"
                     /locus_tag="PW113_003432"
     CDS             <1..>1713
                     /gene="fss3"
                     /locus_tag="PW113_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2527656.1"
                     /translation="HYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKNPLPDMSDKAKLV
                     SVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAIEAKINQAIADYQ
                     KKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVNGNQLIISPNANS
                     KESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVEMEGSLKIKKVDK
                     ESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTITEKSVPAPYTIDT
                     TPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSLAGNTFAIRKDSP
                     TGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTFKPVKVELNYVNQ
                     TVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTLFTVKDGKAVKWT
                     ENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAPEGYTLDETKYPV
                     SIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGFNDLTFKVSPLEG
                     TNEIT"
BASE COUNT          602 a          313 c          379 g          419 t
ORIGIN      
        1 gcattatgca gtgtacaacg gcgttaaaca agatattttc tgtgttgaac caggagtccc
       61 gatttacaat gagttcactc ctggatatga gaagaacccg ttaccagata tgtccgataa
      121 agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata ctcatattgt
      181 ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa taaaacgatc
      241 agatggtagt gccgtaaata tcgcagccat tgaggcaaaa atcaatcaag ccattgccga
      301 ttatcagaag aaaccaagct tccataatag tacggctaaa acagtattag ggcaatcgac
      361 tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg tggaaaatac
      421 cgcaaacatt gattatcgtg tgaatggcaa tcagttgata atcagcccaa atgccaattc
      481 aaaagaaagt ggtgtgttga cgcttaagaa atctgctggt accggaacac ctgtcgccta
      541 taaaaaggct ggccaacaga ctttaatggc cggggcgatc gataagccga acacttatac
      601 ggtcaaaatt gatgtggaaa tggagggttc tttgaagatt aaaaaagttg ataaagaatc
      661 gggtgatatt ataccaggaa cggttttcca tttagacttt ggaaaaactt tacccgcaaa
      721 agacgtgacc accgataaag aaggcattgc gaccttagat gagattcctc atggtacaaa
      781 ggtaaccatt actgaaaaat cggtgccggc gccttatacg attgatacca cacccatgac
      841 tgccacaatt aaagcaggtg aaaccatttc agtgacttca aaaaatacgc gagaaaaagg
      901 tcaaattatt ctggataaaa ccggaattga aacagggagt gatctttgga acgataatta
      961 ctctttagcg ggcaatacgt ttgccattcg caaggatagc ccaactggtg aaatcgtcca
     1021 agaaatgacc acggatgaaa aaggtcatgc agaaacacca aaagagattg ccaatgcgtt
     1081 ggaattggga acctactatg tgactgaaac caaggctagt catggctttg tgaatacttt
     1141 caaaccagta aaagtcgaat tgaattatgt caatcaaaca gtggctcttg ttaccagtaa
     1201 cgtaaaaggg caaaaccaag aaattactgg tgaaaccact ttgacaaaag aagacaaaga
     1261 taccggtcat gaaactcaag ggaaagctga gtttaaaaga gctgaataca ccttgtttac
     1321 tgttaaggat ggaaaagcgg tcaaatggac cgagaacttt aaaccagaat tgttgaaggg
     1381 aacgaaagct tctgatgaaa ccgtaacttt gactttagat gaaaagaatc aagttgccgt
     1441 taaacactta gcgattaatg agtattactg gcaagaaacc aaagcgcctg aaggatatac
     1501 cttggatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg aaaaaaatgc
     1561 tgtgattact cgagatgttg cggcaaaaga acaagtgatt cgctttggct ttgatttctt
     1621 taaatttgct ggatcaacta ctggcactgc cgaaactggc ttcaatgatc taacctttaa
     1681 agtgtcgcca ttggaaggaa ccaatgaaat cac
//
LOCUS       DANKHJ010000256         1702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000256 DANKHJ010000000
VERSION     DANKHJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(60..278)
                     /locus_tag="PW113_003433"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="PW113_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="PW113_003434"
     CDS             complement(544..738)
                     /locus_tag="PW113_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527657.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="PW113_003435"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="PW113_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          596 a          250 c          226 g          630 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cc
//
LOCUS       DANKHJ010000257         1685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000257 DANKHJ010000000
VERSION     DANKHJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(74..496)
                     /locus_tag="PW113_003436"
     CDS             complement(74..496)
                     /locus_tag="PW113_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2527658.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(514..834)
                     /locus_tag="PW113_003437"
     CDS             complement(514..834)
                     /locus_tag="PW113_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527659.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1127..1318
                     /locus_tag="PW113_003438"
     CDS             1127..1318
                     /locus_tag="PW113_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527660.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            1332..1583
                     /locus_tag="PW113_003439"
     CDS             1332..1583
                     /locus_tag="PW113_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527661.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
BASE COUNT          545 a          279 c          297 g          564 t
ORIGIN      
        1 cctcacttct tgttataata tatttgtgat ctcagaaatg aggtatgagt ccgtgttgca
       61 gcacgggctt tttttactgt gcataagagt attttttctt gaaatatgac tggcaaacat
      121 taaaacattc tgttcttaac ttattattga tagagtagaa ttccatgaaa ttttctaatt
      181 tgaactgaga ttcatcagtc aattcgttct caataaagat atttagtaaa atcataattg
      241 cgattctatc agcttcagct tcgaattttg aatgaaaagt tgtagagtta tcgtacagta
      301 ctgaatattc aaaatgtgaa gcaatgaaat gaccgagctc atgggctaaa tgaaaagctt
      361 cagaactgtc ttcgtgtagt ttttcattca aaaatactat tcttggtttt ggataataaa
      421 aacctggttc ttccatttcc atatagatta actttaaatt atactcactc agcatttctt
      481 tcaactttaa atacatacaa cccatcactc caactattca ttttcctcta aagctttagc
      541 aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa aagaaaatac
      601 agtatcatct tctgataaat ccacatgttt aggcgtttct ttttcttctc tacctagaag
      661 atagtctaca gagacattga aatagtcagc taccttttgc aaatctcttg ctttgggttc
      721 tgttgttttc catcgataga aaagattttc gctaaatcct aattctaaag cgacttcttt
      781 catgctttta tctctttggc tagctaatga ttttatcctt tcaaacagtg tcataataat
      841 gttctccaat cctaaacaaa taaaagtaca caaaatgtaa attatactgt tgacataagt
      901 ataagtttgt attattattt gtttgtaagt taatttgata gaaaaaaagc aaactaaaaa
      961 tacaccttat agcattaagt ttggcgaccg attgcataat aaagggtttg ttgtaggctt
     1021 atttaactat gtttatatag tatacgattg tatactatta gtcaatagtt tatacgtttt
     1081 ttctatcatt ttgacttaca aatctatatg taaggggagt gaatagatgt caaatctaga
     1141 taacggccgt gctgcaatca agaagtttat gcgagaaaac ggagtaacta tggaagattt
     1201 ggctactgca tatggataca cacgtgttcg aatgcagcaa attattgatg gccattggag
     1261 tggaccaaaa gctaataaaa ctatcttaga aatcatccgt gattacagaa ttcgatagga
     1321 ggaattaatc aatggaacaa ctagctttag ttaacctctc agccctcaaa gcttctttgg
     1381 cagaatctga gattgcaaat gaagtgtggg atacaaagca agctgcggaa tatttgaaaa
     1441 ccacgacacg cacgctaact aaagatgccg aatctggaaa gattccagca gcgaaagttg
     1501 gcagagagtg gaggtttagt agtatcgctt tgtatcagta tctaaaagga ggaaaaacat
     1561 gggcaaattc aacagagcac tagtattcag cgcaccgcta atcatctacg ctttagggct
     1621 ttggggaaat agacaagcgt tgataggaac gattgtgtac atggtttgga tttttatagg
     1681 gcttg
//
LOCUS       DANKHJ010000258         1668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000258 DANKHJ010000000
VERSION     DANKHJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..830)
                     /locus_tag="PW113_003440"
     CDS             complement(<1..830)
                     /locus_tag="PW113_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL2527662.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..1451)
                     /locus_tag="PW113_003441"
                     /pseudo
     CDS             complement(901..1451)
                     /locus_tag="PW113_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT          539 a          288 c          210 g          631 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat
     1141 attcttattt tctctgtaaa caattaaatc ttcatagcta ttaagtaaag catcaaaata
     1201 attgatagca gctttggtat catcattaga atctatatac cttttcaaaa acattttaaa
     1261 aaactcatca gataaattgt gggtttcatt tggagcaaac aaaaaagcta aaaaattact
     1321 atgtcttatt tcatttttag atagcttaag tatctgaaat atatttgttt tcccagtcca
     1381 tttagatagt tttcttcaat atcatccata tcctctaata gattttttaa acactttttt
     1441 tcatctatca tgttttttct ccttgccatt attctaacta aaattttacg tatttgtttc
     1501 tatgtctaca ttatcttttt aacagtatct atttttcttt tgcaacttac ttggctacca
     1561 aagcaaataa aaaagttatt tataaatttc tccctaccac tacctattgc tattatttaa
     1621 aatagggata attttttctc ttaaaaaata tcattatttg tggtatga
//
LOCUS       DANKHJ010000259         1664 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000259 DANKHJ010000000
VERSION     DANKHJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..909
                     /locus_tag="PW113_003442"
     CDS             <1..909
                     /locus_tag="PW113_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HDL2527663.1"
                     /translation="VILVGGSTRIPAVVEAVRKETGKEPNKSVNPDEVVAMGAAIQGG
                     VITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFSTAADNQPAVDI
                     HVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDKNGIVNVSAKDLGTQKE
                     QKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRNDVDALLFSVDKTLKELE
                     GKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIVQNLTVKLYEQAAQQQAQ
                     ENPEAAQGGADDVVDADFEEVDGDDK"
     gene            1060..>1664
                     /locus_tag="PW113_003443"
     CDS             1060..>1664
                     /locus_tag="PW113_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HDL2527664.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHG"
BASE COUNT          576 a          297 c          391 g          400 t
ORIGIN      
        1 gttattttag tcggtggatc tactcgtatc cctgcagttg tagaagcagt acgtaaagaa
       61 acaggaaaag aaccaaacaa atcagttaac cctgatgaag tagttgctat gggtgctgct
      121 atccaaggtg gcgtgatcac tggtgatgtg aaagatgttg ttttacttga cgttacacca
      181 ttgtcattag gtatcgaaac aatgggtgga gtgttcacta aattgattga ccgcaataca
      241 acgattccaa caagtaaatc acaagtattc tcaactgcgg cagataatca accagcagta
      301 gacatccatg tattacaagg tgaacgccca atggctgctg acaacaaaac tctaggaaga
      361 ttccaattga cagatattcc agcagctcct cgtggtattc cacaaatcga agtaacattc
      421 gatattgata aaaacgggat tgtaaatgta tctgcgaaag atctaggtac acaaaaagaa
      481 caaaaaatca cgatcaaatc ttcttcaggt ctaacagacg aagaaatcga acgtatggtg
      541 aaagatgcag aagcaaacgc tgaagcagat aaagctcgta aagaggaagt tgacctacgt
      601 aatgatgtag acgcattgct attctctgtg gacaaaacat tgaaagaatt agaaggcaaa
      661 gtggacgaag aagaagtgaa aaaagcagaa acagctcgtg acgaattaaa agccgctgtt
      721 gaagcaaaca acatcgagga aatgaaaact aaacgcgatg cattgaacga aatcgttcaa
      781 aacttgactg ttaaactata cgaacaagct gcacaacaac aagctcaaga aaatccagaa
      841 gcagctcaag gcggagcaga tgatgtcgtt gatgctgatt ttgaagaagt cgatggcgac
      901 gataaataat cagttcactg aatttcgaga gaaggccaaa gttgctactt tggctttttc
      961 ttttgaaaaa tagtttgaag tatcggtttt tgtttcaaac aaaatatgat atgataacaa
     1021 gcgacgtgaa aatgatgaga accaatagga ggaaagccta tggcaacaaa acgtgattat
     1081 tatgaagtct tagggctctc aaaaggagca tcagaagatg aaataaagaa agcttatcgt
     1141 aagctttcga aaaaatacca tcctgacatc aataaagaac ctgatgcgga agaaaaattc
     1201 aaagaagtat cagaagcata tgagatttta agtgacccgc aaaaacgcgc tgcatacgac
     1261 caatacgggc atgcaggcac agatccaaat tacggtgctg gtggagctgg tggcggattt
     1321 ggtggattcg gcggcttctc tggtggcggc ttcggtggtt ttgaagatat cttcgactct
     1381 ttctttggag gaggcggtcg gtcagtcgat ccaaatgcgc ctcgtcaagg tgctgatttg
     1441 caatatacgg tcgacttgtc ttttgaagaa gcgatttttg gtgtagaaaa agaaatcaag
     1501 tataatcggg aagaaatctg tcatacttgt ggtggaaacg gtgcaaaacc aggcacacaa
     1561 ccaactactt gtcataaatg tcatggtgca ggtacgatca atgtagaacg ccaaacgcca
     1621 cttggacgag tgatgagccg tcaaacttgt gatgtttgtc atgg
//
LOCUS       DANKHJ010000260         1659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000260 DANKHJ010000000
VERSION     DANKHJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..828
                     /locus_tag="PW113_003444"
     CDS             <1..828
                     /locus_tag="PW113_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527665.1"
                     /translation="LSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELD
                     DTSVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVS
                     DPVQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGD
                     SIAVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQE
                     NTSTSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            852..>1659
                     /locus_tag="PW113_003445"
     CDS             852..>1659
                     /locus_tag="PW113_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2527666.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICA"
BASE COUNT          526 a          314 c          404 g          415 t
ORIGIN      
        1 ttgagcagtg ctgtcacagc aaagatcgaa ccatcgacta ttacagtaac ggttgagaaa
       61 aaggtaataa aagatttcga tgtggaaacg ttgattcctt caacgttcac accatctgga
      121 tatgaacttg atgatacttc cgttagtcct aaaaaagtct ctatcactac aggtgacaaa
      181 acattggcag aaatcaaacg agtagtagct gatgtggacg cttcgatggt cacagatgat
      241 gggattaata gtgaggtccc aatacaggcg ctgaatgcag caggggaagt attaagtatc
      301 gtctctgatc cagtgcaagt aacagtgaga gctgattcga tcaagccaag caagtcggta
      361 cgactttatg ggatacagca aggaacacca gctgccgggg tagaaagcta tgatttcagt
      421 ttttcaaaac tggaagcaga agtaagcggc tctagcgatt tgcttgcatc gatcggtgac
      481 agtattgctg taccgatcaa tgtctcaggt atcaatcatc gaacaactcg taaaattgaa
      541 ataccagtag aagaggggtt aagtatcaag cccgaatcag ttagtgtgga gattacaccc
      601 gtgttgcaaa cgtcaagcag tagctctgct tcgacgagca gtcggacaca agaaaataca
      661 agtacgagta gaagtatttc atctgctgat tcaggaacgg aaccatcgac aacaaccatt
      721 tcttccagtt cggaatctgg gacaagcagc tcgaccacaa gtacagaaga aacatcagaa
      781 acaagcgaat caacacaaat gagcagaaca aattcctcag aaaattaagt taaaggagta
      841 attttacaac tatgggtaaa tattttggaa cagacggcgt acgcggtgaa gccaacaagg
      901 aacttacgcc agaacttgca tttaagctgg gacggtgcgg aggttatgtc ctaagccagc
      961 atgaagaaga aggaaaacgc ccacgcgtgt tagtagggcg ggatacaaga atctcaggtc
     1021 aaatgttaga agaagcgctg atttcaggac ttttatcggt gggtatcgaa gtgttccagc
     1081 taggtgtgat ctctacacca ggtgttgctt atctgacacg tttgcaaaaa gcaagcgcag
     1141 gggtcatgat ttctgcttct cataatccag cacaagataa tggaatcaaa tttttcggtg
     1201 cagatggttt taaactagta gatgaccaag agctagaaat tgaagcatta ttagatgctg
     1261 aagtggatga attgcctcgt ccatcagctg aaggattggg aactgtagaa gaatttccag
     1321 aaggcttatt gaaatattct caattccttg tacaaacgat caacggtgac ctttccggtt
     1381 tgaccatttg tgtcgatgca gcaaacggag caacttcaac aagtgttaat cgtttgtttg
     1441 ccgatcttga aacagatttt tataccatgg gcacaaatcc agacgggtta aatatcaatg
     1501 atggcgtagg ctctacccat ccagaacgtt tagctgagat ggtcgttgaa aaaggagcag
     1561 atgctggact agctttcgat ggcgatggcg atcgtattat tgcggtggat gaacttggaa
     1621 acattgtcga cggtgataaa atcatgttta tctgtgcta
//
LOCUS       DANKHJ010000261         1651 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000261 DANKHJ010000000
VERSION     DANKHJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..567)
                     /locus_tag="PW113_003446"
     CDS             complement(<1..567)
                     /locus_tag="PW113_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2527667.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPY"
     gene            complement(568..1350)
                     /locus_tag="PW113_003447"
     CDS             complement(568..1350)
                     /locus_tag="PW113_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527668.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(1343..>1651)
                     /locus_tag="PW113_003448"
     CDS             complement(1343..>1651)
                     /locus_tag="PW113_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527669.1"
                     /translation="GILHEGHLIKELSKEELAQESRQFIKIDTSATEQAVTVLDSLGY
                     RDYFVQSSRVIQLFEGIDQVAAINQALVEAKVPVDGIHLVGQKLEDYFLQLTGGNPHV
                     "
BASE COUNT          503 a          362 c          297 g          489 t
ORIGIN      
        1 atatggttct tgccaaactg tttcataaag atttgctcgc gaaaatactt ttttcggatg
       61 ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc aaatggacag ccacaccatt
      121 cacatttaca tccctcgact ctttgtccag taagatttcc ttgtaataga tgcgttgcga
      181 cagatctttt acttggacag aagtctgatg acgaagttgg atgccgattc gtaacaacag
      241 ttcttcgata tcaaatggtt tcgtcacata gtcatccgct cctgctgcca acaaggaaac
      301 tttatctctt tgatcgcttt ttgctgaaag aacgatgatc ggaacattcg atgtctgtcg
      361 gatttctgct aacaatgctt cacctgaaag ccctggaagc atgagatcta gaaggatcaa
      421 atcgacagga tatgtgctta acaataattt tccttccgta cctgaataag catcaagaag
      481 tttgtatctc tgttcaagaa cctcctccag taaagaatga acagcttcat cgtcttcgat
      541 aatcaaaata gtttccagca ggaacaccta cttaatgtca gatttttgga aaataaacat
      601 tcctagagct gatgatacca cgattgttac tagactgacc agaatcattt tcggtgcttg
      661 cgaataagtt gcttgttgcg caaagtccgt atacatgagc cacgtttccg taaaccactc
      721 cgttagaaat tccaagcctt tgatttttgc gaaaacaccg ggaacgaagg ccgttcccat
      781 gatataaaga aaagaaagaa gcaaactgac actggtactt ctagtgatga atgtcaaagc
      841 cactggaaca gataatgtcg ctaatatcag cagaagcaat acaggaaata cttttagaac
      901 ataatttcca tagtttttga ttccctcaac ttcaccaaat ccgaaagcaa gtgtgtaagc
      961 aatcatggct agccccgtaa tcaaaaccac gccaagtgtt gtgatcaaaa gcgtaacgag
     1021 aagtttcgac acataataag tcattcgatt ccgtcctaaa gataacgtat tgcgaatcgt
     1081 accgttttga aattcttccc caatataaaa gcacgcaaat gctgcgaaag ccagaagaat
     1141 ccatgcagcc attttttcca ccattaaaat ccctgttgca ccgttgatga ctgtatccgt
     1201 tgcttggttt aattcattgc cgtattttgt atgcatatac cattcgttcc aagaataaaa
     1261 aagcggaagt acgaccatca gtcctagtga gacccacatt ttacggctct tccatacttt
     1321 tattttttca gcagttaata gattaaacat gtggattgcc tccagttaat tgtaagaaat
     1381 aatcttccag tttttgccca actaggtgga taccatccac aggaactttt gcctctacca
     1441 atgcttgatt gatggctgct acttggtcaa ttccttcaaa aagctggata acacggctag
     1501 attggacaaa ataatcccta taaccaagag agtcgagaac cgttactgct tgttccgtcg
     1561 ccgaagtgtc aatttttata aattgacgag attcttgtgc caattcttct ttcgataatt
     1621 ctttgatcaa gtgtccttca tgcaaaatac c
//
LOCUS       DANKHJ010000262         1623 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000262 DANKHJ010000000
VERSION     DANKHJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(213..410)
                     /locus_tag="PW113_003449"
     CDS             complement(213..410)
                     /locus_tag="PW113_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527670.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(486..686)
                     /locus_tag="PW113_003450"
     CDS             complement(486..686)
                     /locus_tag="PW113_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2527671.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(806..>1623)
                     /locus_tag="PW113_003451"
     CDS             complement(806..>1623)
                     /locus_tag="PW113_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2527672.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
BASE COUNT          479 a          329 c          284 g          531 t
ORIGIN      
        1 atccagccat tctttagcca gcgatctacc tcatcaaaac aaaaaatttt cctctgatgg
       61 ttccttaaat aagactattt tgatcctttt tccatgaata agattttgca tcagacaata
      121 cttcatttct gaaaaagttg cggcaccaat atagcctaat acccctttcc gatcttgatt
      181 aaatacaaaa aataaatccg ctgctgcgat atttaaaaaa aagtcgtcaa gatttctgga
      241 tactctttgt ctaggatcgg acatgagcga ggataatcaa ttaattcaat gcctttttta
      301 ctgaaatctt tttccatatc tcgatattct tcttggaaaa caatacttcc agaaataaca
      361 gctttcacaa aattggcctc cattttttta tttttcagac actccaccat accggatacc
      421 tctcaaagaa aagacatagc aaaaatcaat agcagctatc tctccacggt tttttactgg
      481 taattttatt tttcttttgg aaattgtatc aggaaggtcg ttccttcatt ttttcggctg
      541 gtcacttcaa ttgaaccgtg gtgcaggcgc actagttcct tcacgattga taatccaaga
      601 cctgactcgc ctatctttgt atttttactt gacggatcgg ctttataata gcgatcccaa
      661 atgttttttt gttcctcttc aatcatgccg atgccattgt cctcgacagt aataaataca
      721 ggctctttgt caaatgatgt tggtaaagag aaatcttaga ataatttgga cagaacagaa
      781 agaagaaact ctctttctgt tctgtttatt tggttaaaaa tgttttgcca tgacgaatca
      841 tgatccgtag tttgaaattg taaaagctgc gaaagccgta cgcattgcgt tttaatactt
      901 tgatgtggtt gtttaaacat tccaatggtc catttgaata gctgtagtga agcgcatttt
      961 ggatctgtgg cagaaacttt ttaaacgttc gtaaagtggt tgtataggtt tcaggaagac
     1021 cgctgacttc tctgtttaaa cagtcctcaa ataatgactg atctcttttc tttaccgcat
     1081 aaagaatgtc ttggtataac tgatagcctt ggcgcaggtt gggacaagca gataacagtc
     1141 gatcgacaat ctcactttca gtcaaataat ctttaaaact ggcacgccaa accctttttt
     1201 cgtagtttaa ctttgcttct tctttttgta ggagtttcca atagcgtttc aattttttcc
     1261 ctaaccctcg ttgagagtgg gaagttcctt taagccgtgc cgtggtttct ttcactcgat
     1321 gattcaagaa ggttctgcca atatgttgga cgatgtggaa acggtcgatg atcaactgag
     1381 cattcgggaa aagctttttc accaatgaaa cataaggcgc atacatatca ataacaatcc
     1441 attgaacggc ttcacgagtc gctaatgaaa accgagaaaa atagcattct aaataaggca
     1501 agcgtcgatt ttcgacaata tccaataatg catggctttg tccgtccatc atgataaaac
     1561 tcattgcact ggcaacactt cgaaccgatt tgaattcatc aaagcataag acctttggca
     1621 aag
//
LOCUS       DANKHJ010000263         1616 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000263 DANKHJ010000000
VERSION     DANKHJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..416)
                     /locus_tag="PW113_003452"
     CDS             complement(<1..416)
                     /locus_tag="PW113_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HDL2527673.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGP"
     gene            complement(460..1290)
                     /locus_tag="PW113_003453"
     CDS             complement(460..1290)
                     /locus_tag="PW113_003453"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2527674.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(1305..>1616)
                     /locus_tag="PW113_003454"
     CDS             complement(1305..>1616)
                     /locus_tag="PW113_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2527675.1"
                     /translation="IIDVMNLFGASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTV
                     EIAEVGIPQIKIPVKTGRNVAIIIEVAAMNFRARTMGFDATKTFEERLSRLIEENSGN
                     D"
BASE COUNT          486 a          349 c          292 g          489 t
ORIGIN      
        1 ggtccagaaa gaacaacaac agcttttctt ttttctgctg ggatttcctc taacaagatg
       61 tcagagatcc ttttatgtgt tccctgttct aatcctttac ttgcatggat gatcactggc
      121 tttgtattca atttaggcac tagttctttt gcaacagaac gtattgcttt cgttggaatg
      181 acgaatagca ccgcatctgc gtcttttact gcatcttcca tattggtgaa tgcttggatg
      241 gaatcgggta gataatgatc tggtaaataa cgttgattcg tgtgttgggt gttgatttct
      301 tctacttgtg aagcacgatg tccccaaatc cgaacgtcat gtccgttttc ggcgaggact
      361 tgtgccaaag cagtacccca agagcctggt cctaaaaccg ctactctata attcatttta
      421 ccactcctga aatttttttc atttttaacg ctctaaaaat cagataattt gttgattttt
      481 tcctaatgag cggtcataat ccggtaaatt tccttttttt ctgcgccaaa taaatagaat
      541 caatgtaccg acaaataaca cagcagataa taattgagaa atacggatca cacctaaaaa
      601 gtaaagactg tctgtacgta atccttcaat aaagaaacgc ccgaaactat accacattag
      661 ataacataaa aggacttcgc cttttttcaa aaggtgtggt ttctttcgta aaaacaaaag
      721 caaaataaat ccaatcaaac tccaaagtga ttcataaaga aatgttggtt gacgatagat
      781 cccttcgata tacatattat caatgatgaa ttgcggtaaa tgcagacttt ctaagaagct
      841 tcgcgtcgtg tctggtccat atgcttcgtg gttcatgaaa tttccccaac gaccgattgc
      901 ctgtgctaat aatacacttg gtgcagcgat atctaaaaaa gtccacaaag aaataaaatg
      961 atatttagta aaaaaataca gcgtgatcgc gcctgcaatc agtccgccat aaatagctaa
     1021 gcctcctgaa cggatattga atatttggat tggatcagct atatactggg gaagatcaaa
     1081 taaaacataa tataagcgag cgccaataat ggagataggc aatccccaca acataaaatc
     1141 tgtcacatca tctgctttta atcctacccg aacagcctct ctgctgctaa gccacatagc
     1201 gataatgata cctgatacga tgatcaatgc ataccagtag ataggaatcc ctacaagctc
     1261 aaaagcaact cgattgattt gtgctaacat attgtcactc cttgctagtc gttgcctgag
     1321 ttttcttcaa tcaagcgact taatctttct tcaaatgttt tcgttgcgtc aaagcccatt
     1381 gtacgtgcac ggaaattcat cgcggctact tcaatgatga ttgcaacgtt tcgtcctgtt
     1441 ttgacaggga tttttatttg tggaatgccc acttccgcga tttcaacggt cgtgtcttca
     1501 gagcccagac ggtcgtattt cttatctttt gcccatgctt ccagataaac agccaactgg
     1561 acttgcatag agccacgtac cgcactcgca ccaaaaagat tcattacatc aatgat
//
LOCUS       DANKHJ010000264         1608 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000264 DANKHJ010000000
VERSION     DANKHJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(248..1018)
                     /locus_tag="PW113_003455"
     CDS             complement(248..1018)
                     /locus_tag="PW113_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2527676.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(1011..>1608)
                     /locus_tag="PW113_003456"
     CDS             complement(1011..>1608)
                     /locus_tag="PW113_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2527677.1"
                     /translation="MKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRL
                     LILDEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELI
                     ENTSMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFD
                     QLTRQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
BASE COUNT          507 a          365 c          283 g          453 t
ORIGIN      
        1 aataaatggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg
       61 aggagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      121 ctccactaaa cggtgttcaa tcagctgacc ctcggtattc gctcaaaaaa caacaaaata
      181 tgaggaacta ttttcccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac
      241 ctcttttcta acttctcaat cctcttcctt tattgatcaa ataccaagta acggcgtaca
      301 aaacaacgat gaataacaca agtacgatca tcgatgttgc tactggcaca tcgatcgttc
      361 caaggaatcc gtaacggaaa ccggagatca tataaacgat cggattgact tttgaaacag
      421 cttgccaaaa gggcggtaac atagagatgg catagaaaac gccacctaga taagttaacg
      481 gctgcaagac aaatgtaggc acgatcgata catcatcata agactgagca aaaatcccgt
      541 tgagcagtcc tgctaatgaa aacaagattg ccgtcatcaa taatgtaatg atgaccatga
      601 accatgaata cacatgaagt ggtacaaaga acaaggaaat gatcgttacg agagagccga
      661 cgagaatact gcgacccaca ccaccaatca caaagcccca aataatcaca tgcgtaggaa
      721 caggtgctac caagatttct tcgatatttt tctgaaattt ttgagagaag aaggacgaag
      781 aaacattggc atacgaacta gtgatagcag acatcataat caaaccagga acgatgaatt
      841 ccatatatga aaaaccaccc atgtcaccga tccgcccacc gatcattttt ccgaaaataa
      901 caaaatacaa ggatgtcgtg atcactggcg gaaccagtgt ttggacccat atacgcaaat
      961 agcggttcgt ttccttgata gctaaacttt ttaatgcagt aaaataaaga ttaaacattt
     1021 ttctcctccg tatgattatt ttcttctgtg attttcaaga acagttcttc taaacgattc
     1081 gacttattcc tcatagaaag aactttgatt ccttgtcttg taagctgatc gaagattccg
     1141 ttcacccctt gatttcgttc aacttcgaca gcgattgtct gctcatcttc aaatgtacaa
     1201 gcatatcctt caatcacagg tttggtatca tatggcgcta aatcaaagat aaatgtttca
     1261 aattgcagtt ttgacagaag ctctttcata cttgtattct cgatcagttc acccgattgg
     1321 atgattccga tgttgcgaca aagcatttca gcttcttcta gataatgcgt cgtcaaaatg
     1381 atcgttgtac cattttcgtt caattctcgt agaaactccc acatctcgcg tctcaactcg
     1441 atgtcaactc ctgctgttgg ttcatccaag atcagcaaac gtggttcatg catcaatgcc
     1501 ctagcaatca tcagtcttcg cttcattccc ccggaaagca tacgtgcacg gacatttctc
     1561 ttttcccaaa gattggactg cttcaaatat ttttcgctgc gtttcatc
//
LOCUS       DANKHJ010000265         1597 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000265 DANKHJ010000000
VERSION     DANKHJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            1..1232
                     /gene="aph(2'')-I"
                     /locus_tag="PW113_003457"
                     /pseudo
     CDS             1..1232
                     /gene="aph(2'')-I"
                     /locus_tag="PW113_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1216..>1597
                     /locus_tag="PW113_003458"
     CDS             1216..>1597
                     /locus_tag="PW113_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2527678.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEK"
BASE COUNT          654 a          128 c          272 g          543 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt attgatgata atatggtttt tgatattgat
     1261 aatttaaaag gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat
     1321 aaaattatag gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt
     1381 tatttacact caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta
     1441 ttatctttta ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact
     1501 gataaaggta atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat
     1561 aaagatgaaa tagtatatgt atatgattat gaaaaag
//
LOCUS       DANKHJ010000266         1592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000266 DANKHJ010000000
VERSION     DANKHJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.278"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..1190)
                     /gene="asnB"
                     /locus_tag="PW113_003459"
     CDS             complement(<1..1190)
                     /gene="asnB"
                     /locus_tag="PW113_003459"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2527679.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGR"
BASE COUNT          483 a          312 c          254 g          543 t
ORIGIN      
        1 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
       61 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
      121 ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa
      181 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
      241 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa tagttcggtt
      301 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
      361 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
      421 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
      481 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
      541 tgctgaatgt ctagttttcc atctttatac gtataataat ggccttcagg caaacgatag
      601 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
      661 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
      721 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
      781 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
      841 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
      901 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
      961 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
     1021 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
     1081 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
     1141 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
     1201 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
     1261 cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg
     1321 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctacc
     1381 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa acagcaaaaa
     1441 aagacacgac ctaaagatcg tgtctaatta tgactgtatc agaaaatatg ataccaaacg
     1501 gatctattca ttaattaatt gacatagttt atctgttgct cttttatgac tggataactg
     1561 accaattcat gacgatccaa gtcttcttta tt
//
LOCUS       DANKHJ010000267         1591 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000267 DANKHJ010000000
VERSION     DANKHJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..449)
                     /locus_tag="PW113_003460"
     CDS             complement(<1..449)
                     /locus_tag="PW113_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2527680.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFV"
     gene            complement(564..1421)
                     /locus_tag="PW113_003461"
     CDS             complement(564..1421)
                     /locus_tag="PW113_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2527681.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
BASE COUNT          485 a          343 c          254 g          509 t
ORIGIN      
        1 acaaaaggca cgataactga taaaccgata aaaccataaa cgacagaagg aatacctaca
       61 agcagctcga tgaccggctg taagatcttc gttccgtttt taggagagat ctccgtcatg
      121 aatactgctg caccaatggc aaatggcgtg gcaacgatag cagataaaaa ggtcacaata
      181 aacgaacctg tgatcatagg caatgcccct accattggtt tcccatttgc tccgatttgg
      241 cttggattcc aatctgtacc aaacagaaaa tcaaatacat tgatcttatc agtaaaaaag
      301 gtggccaatc ctttactagc tacaaaataa aaaatagata atacaaccaa cacgatcagc
      361 gcgatacata aaaaactgat gaattttcca cgctgttcca tttttgaacg ttttgatttt
      421 gttaataata cttttttcac atcttccaca gatgactccc ccttaatcag gtttaacata
      481 ctcactcagt gtacaaattg aacatcaatt tggttttaat aacatgtaaa agtttagcta
      541 agtttgtaaa ttttgtgtaa agattactct ttaacgatat ttccttgcca atcacgttcg
      601 actttcatat gagaaacagg gatataacct agttctccaa cgatgttatc ttgtacttca
      661 tcagataaga tatagtctaa aaattctttt gtcagttttt ttggatgatc tcttgtgtac
      721 atatgctcat atgcccaaat cgtccatttg ttcgttgtca cattttcatc tgtcggttct
      781 acaccgttaa cattcaacgt atctacatca tcggaaacat aagaaaaagc aacatagctg
      841 attgcaccgg gtgtgtcggc aacgatctgg cgcaccattc cgcttgaatc ttgttcttgt
      901 gcctgaacag ctgtttctcc gtccaatacc catttttcaa acgtgctacg ggttccgctt
      961 cctgaagcgc ggttcaatat aactactggc tgatcttttc caccgacttc actccagttt
     1021 ttgatctcgc ctgtaaatag ttttttcagg ttttccatac tgatgtcttt tacgccaata
     1081 tccttgttca caataggagt gatcccaaca actgctactc gatgatcgac taatgctgag
     1141 gcatcgatcc ccgatttttc ttccgcaaac aaatcagagt ttccgatgtc gactgctcct
     1201 gactgtactt gagacaaacc agttccgctt ccgccccctt gtacattgat gaattttcca
     1261 ggattttctg tctggtaagt ttcagcaact gtttctacta aaggctgtaa agcagaagag
     1321 ccaacagccg taatggattc tcctcgatca atccactttg aacagcctga taaaagaata
     1381 gctaatccta ttattggtaa gaataattta agttttttca ttagaaatct cctttaatcc
     1441 taaagtatgt ttattttatc attttcatta cttgatgaaa acgaatcaaa atcattaaat
     1501 aacaaaataa attctcataa ttgtaaaaaa aactatctca ttaaaagttt ctatgctata
     1561 ctggctttga aatgtgtctc ttttaatagc a
//
LOCUS       DANKHJ010000268         1587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000268 DANKHJ010000000
VERSION     DANKHJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..663
                     /locus_tag="PW113_003462"
     CDS             <1..663
                     /locus_tag="PW113_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2527682.1"
                     /translation="SINEGDVVCIIGPSGSGKSTFLRCLNRLEEPSDGEIIIDGAHLM
                     DKSTDINLVRQHIGMVFQHFNLFPHLSILENIILAPMDLKKESREEAEKKAIKLLQTV
                     GLEDKKDMYPEMLSGGQKQRVAIARALAMNPDIMLFDEPTSALDPEMVGDVLNVMKKL
                     AEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFLEDGTPQQIFENPQNERTKDFLNKVL
                     NI"
     gene            complement(708..881)
                     /locus_tag="PW113_003463"
     CDS             complement(708..881)
                     /locus_tag="PW113_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527683.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            857..1522
                     /locus_tag="PW113_003464"
     CDS             857..1522
                     /locus_tag="PW113_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527684.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
BASE COUNT          508 a          314 c          298 g          467 t
ORIGIN      
        1 tccatcaacg aaggagatgt cgtctgtatc attggccctt caggttcagg aaaaagtaca
       61 ttcttgcgtt gcttgaatcg tttagaagaa ccttctgacg gtgaaattat tattgacggc
      121 gcccacttga tggataaaag cacagatatc aacttggtga gacaacatat cggaatggta
      181 ttccaacact ttaacctatt ccctcatctt tctattttgg aaaacatcat tttagcaccg
      241 atggacctga agaaagaatc tagagaagaa gcagagaaaa aagcaatcaa attactgcag
      301 acagttggtc tagaagataa aaaagatatg tatcctgaaa tgctttctgg tggacaaaaa
      361 cagcgtgtgg ctattgctcg tgctctagcg atgaaccctg acatcatgct ttttgatgag
      421 cctacttctg cactcgatcc tgaaatggtc ggtgatgtct tgaacgtcat gaaaaagtta
      481 gcagaacaag gtatgacgat ggttatcgtt acgcacgaaa tggggttcgc taaagaagtg
      541 gccaatcgcg tcatgttcat tgacgacgga aactttttag aagatggaac gcctcaacaa
      601 atctttgaaa atccgcaaaa tgaacggaca aaagatttct tgaataaagt attaaatatt
      661 taataaaaac caaaaatgca cggtcgccat gaagggaccg tgtattttta tcttcttatt
      721 ttctcaccta atcgtatcag ccgcatcgct tgttttcctg cacaaacaag caatcgctct
      781 acctgttcca tcgcataaga aagtaccatc ggttcattga tgatatcaag tacatagtcg
      841 ataccaccct cacttaattg agcttgattt ttcttctcca tattatgtag ttgtgggaca
      901 actttctgac aatcagcaat tgtgtcaaga acggatcaat cctttattgg agttgctcga
      961 aagggtcttc tcttattacg gacaggcttt gctcactgta catagacaac aaatcattat
     1021 cctagtcaat cggatttcca gagcctcatt gctatcattg cttgataaaa tccagacaaa
     1081 gtttaataaa atgtatcagt tacagctaaa ctttggaatt gggtcgcttt gttatacaga
     1141 gcaagagaca ccgcaatctt tccttcatgc caaacaagtt tgcgaatgga tagctttcca
     1201 tcaatctgtg aacgagatcc gcttttttga agatttagac ctcgggatcg ttttaccagc
     1261 aatcccgtca gatcaacgaa cattatatgt aaaacgtatt ttgaaaagcc tgacagaaga
     1321 agaagtacat ttgttcaaga agactttggc atgcttcagt aaaaataacg gaagcatcaa
     1381 aaattgctca gaagaattat ttattcataa aaacaccttg caatatcgat tgaacaagtt
     1441 ccattctctc actggttata cccctagaaa ctatgacgat tatcatattc taaagctggc
     1501 ttttttgttg gtacaaacat gacaactaca aaaaaaccag gcgaacatat gattgttcgt
     1561 ctggttttta attattaatg acttagc
//
LOCUS       DANKHJ010000269         1582 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000269 DANKHJ010000000
VERSION     DANKHJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..563)
                     /locus_tag="PW113_003465"
     CDS             complement(<1..563)
                     /locus_tag="PW113_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2527685.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAP"
     gene            complement(563..>1582)
                     /locus_tag="PW113_003466"
     CDS             complement(563..>1582)
                     /locus_tag="PW113_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2527686.1"
                     /translation="VLAIVEDMKRLCPNAWLINFANPSGMVTEAVIRYGKWDKVIGLC
                     NVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTAELIDRLYDEDNG
                     LPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTIGTRAQQVKETEA
                     ELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIVVSTKNDGAVPDL
                     PADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEAAVTGDYGTALQA
                     FTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQDELARELSSNTH
                     VMEEV"
BASE COUNT          419 a          380 c          262 g          521 t
ORIGIN      
        1 aatggtgcat gaaggatcat attgatctca ttgaatgtca accaatattt gacaagtcct
       61 ttatagcgat tgaagatgac tcggcacaga ttttcataaa aaccaacgag ttctctggaa
      121 cgccacgcac catattcttt gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca
      181 agcggctcga tcccgtactt ttgacattct ttaaagacat cctcataaaa tttcaaacca
      241 gattcatttg gctctttttc atcgcccatt ggaaaaatac gactccaagc aatggacatc
      301 cgataagtct tgaaccccat ttctgcaaat aaagcaatat cttccttata atgatgatac
      361 atatcgatcg caatttttgc tggataaaaa tgattttcat caaattcaaa cattttcttt
      421 tttccagtaa tgacaggaaa acggtcttct ccgattggca caacatctac gttcgctaac
      481 ccacgtccgc cttcatcata accgccctcg cattgattgg ctgctgtagc tccgccccat
      541 aaaaaatctt ttctgaatcc catcatactt cctccattac atgtgtattg cttgataatt
      601 ctcttgccag ttcatcttgg acttcgattc cttctgcttt taaccggctg attgtttcct
      661 tgaattgcgg taaatactgc tgatgtgcaa taaacaactc attcatgatc cgttttgctg
      721 tctttcctga tcgtataagc ggattgatcg taaacgcttg taaagctgtt ccataatcac
      781 cagtcacagc tgcttcgatc gtcagcaatt ccatattctt cattacttgc aaccagcctc
      841 tttcagcagc aggaagggaa ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg
      901 cagtgacttc tactacacaa tctgccggca gatcaggaac agctccatca ttctttgtag
      961 ataccacgat ctgtgtttct ttatttccat agatcgaagc aatcgtttca caggcagcat
     1021 ctgaataata agcaccacct cgtttttgca attgttcagg tttatgatcc aattctggat
     1081 ctttgtataa ttcaaacagc tctgcttctg tttctttcac ttgctgcgct cttgtaccaa
     1141 tcgtttgata ttcttctaaa gcatgttcca gcatttcttc ttcacggtag taatagcggt
     1201 ggtagccaca agggatcatg ttcatttgtt ttaattgttc tttgaaaaac gggatatcaa
     1261 agatattttt cggcaatcca ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc
     1321 ggtcatttcc tttattatct gctactttgt gccaatggaa gtgatttaga ccggcaaact
     1381 tgtagatcag ctcttctttt ttcttgccaa gcattgttgg ttctgtcatc atcgccatta
     1441 ctggcacatt gcataatcct atcactttat cccatttgcc atatctgata actgcttctg
     1501 tcaccatccc gcttgggttc gcgaaattga tcagccaagc atttggacat aaacgcttca
     1561 tgtcttcaac gattgccaaa ac
//
LOCUS       DANKHJ010000270         1571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000270 DANKHJ010000000
VERSION     DANKHJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            97..660
                     /gene="grpE"
                     /locus_tag="PW113_003467"
     CDS             97..660
                     /gene="grpE"
                     /locus_tag="PW113_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2527687.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            711..>1571
                     /locus_tag="PW113_003468"
     CDS             711..>1571
                     /locus_tag="PW113_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HDL2527688.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERT"
BASE COUNT          581 a          283 c          339 g          368 t
ORIGIN      
        1 taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt aagagcaatc
       61 aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca agaagaacta
      121 gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat
      181 tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa agctgagtta
      241 gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga
      301 ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc caaaaaatta
      361 cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg
      421 gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa
      481 gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct tcatcaagca
      541 gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga agtactacaa
      601 gaaggctaca aattacatga tcgcgtgtta agaccaacta tggtcattgt tgcacaataa
      661 ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt atgagtaaaa
      721 ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa ggaaacgaag
      781 caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta tcattcaaaa
      841 atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga
      901 tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta gaaggtaaat
      961 catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga ttcgcagaag
     1021 actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac tttaatgatg
     1081 cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta gaacgtatcg
     1141 tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat cgcgacgaaa
     1201 aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt gaactaggag
     1261 acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg gacgactttg
     1321 ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc attgacttgt
     1381 ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt
     1441 tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga gaatctggac
     1501 cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca gctgacttag
     1561 tagaacgtac a
//
LOCUS       DANKHJ010000271         1541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000271 DANKHJ010000000
VERSION     DANKHJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..939
                     /locus_tag="PW113_003469"
     CDS             <1..939
                     /locus_tag="PW113_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2527689.1"
                     /translation="VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNS
                     QWMIGGDGWAYDIGFGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFS
                     ASGKYASKKDLGMMAMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCI
                     THGLLGGMKESLKEAKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMR
                     KQVRFSSLENANPNLAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPV
                     KTRKEKTTDLEAEERRAARAERRKKMEQATKEKKEK"
     gene            1128..>1541
                     /locus_tag="PW113_003470"
     CDS             1128..>1541
                     /locus_tag="PW113_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2527690.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYM"
BASE COUNT          535 a          274 c          332 g          400 t
ORIGIN      
        1 gtacacatgt ttgagagcga aggaacgcag caaagagcag caaaactgca agctgcgatc
       61 gaaagcgaac gatcaattgt ccctgaactg gaggatatat acaagagaaa ggatctattc
      121 gtaaaaaaca gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc
      181 gggatcgatc atgtacttgc cagcggagca gacgtgaata ttcttgtttt ggataacgaa
      241 gtctattcca atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa
      301 ttttcggcta gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat
      361 ggaaatgttt atgtggcgca aattgcttca ggtgccaatc aaatgcagac gataaaggct
      421 tttgaagaag cagaaaagta tcctggacct tctttaatca ttgcctatac gccttgtatc
      481 acacatggtt tgcttggtgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct
      541 tctggctatt ggtcattata tcgatataac cctgcaatgg tcgaaaaaaa caagaaccca
      601 atgactttgg actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa
      661 gttcgctttt cctcgctaga gaacgcaaat ccaaatcttg caaaagcttt atttgaaaag
      721 actatccaag acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa
      781 aaaatcaaga aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa
      841 gaaaaaacga cggaccttga agcagaagaa agaagagctg ccagagcaga acgacgtaaa
      901 aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata taatgaataa
      961 ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact tgcaattttc
     1021 ctgaaaatta tgcgtttttc tcaagaaaaa tacgagcaga tcagcctcta aaattaaaac
     1081 atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg tcatttcaaa
     1141 tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac ttgttctctc
     1201 tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta tacaaactga
     1261 tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct atttttattg
     1321 ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg atgaaccaag
     1381 tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc cggagaggac
     1441 tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag caggaagatg
     1501 agaagatgat cttgtctttt gacaaagcca tccaatatat g
//
LOCUS       DANKHJ010000272         1536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000272 DANKHJ010000000
VERSION     DANKHJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            91..540
                     /locus_tag="PW113_003471"
     CDS             91..540
                     /locus_tag="PW113_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527691.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="PW113_003472"
     CDS             540..1034
                     /locus_tag="PW113_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527692.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="PW113_003473"
     CDS             1048..1479
                     /locus_tag="PW113_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527693.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          580 a          234 c          329 g          393 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacga
//
LOCUS       DANKHJ010000273         1526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000273 DANKHJ010000000
VERSION     DANKHJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..734)
                     /locus_tag="PW113_003474"
     CDS             complement(<1..734)
                     /locus_tag="PW113_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2527694.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="PW113_003475"
     CDS             complement(731..1150)
                     /locus_tag="PW113_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2527695.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="PW113_003476"
     CDS             complement(1220..1360)
                     /locus_tag="PW113_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2527696.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1353..>1526)
                     /locus_tag="PW113_003477"
     CDS             complement(1353..>1526)
                     /locus_tag="PW113_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527697.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          417 a          327 c          236 g          546 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccact
//
LOCUS       DANKHJ010000274         1513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000274 DANKHJ010000000
VERSION     DANKHJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(143..457)
                     /locus_tag="PW113_003478"
     CDS             complement(143..457)
                     /locus_tag="PW113_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527698.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            743..982
                     /locus_tag="PW113_003479"
     CDS             743..982
                     /locus_tag="PW113_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527699.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          529 a          266 c          209 g          509 t
ORIGIN      
        1 taaactctag agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc
       61 tagaacaatt aaaacagcag gcttcacatt cgctatacat ttgtaagaag taataattcc
      121 tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac acaagaagta
      181 gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt gacatggaaa
      241 cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa ataacccctc
      301 cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt cgtttggttc
      361 taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga agtaataata
      421 ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct agcaactatt
      481 taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac ttataaattt
      541 tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc aatgtatctg
      601 gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata gatactataa
      661 aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta tgctaaacga
      721 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
      781 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      841 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      901 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      961 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
     1021 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
     1081 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
     1141 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
     1201 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
     1261 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
     1321 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
     1381 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
     1441 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
     1501 acacaatttt cta
//
LOCUS       DANKHJ010000275         1508 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000275 DANKHJ010000000
VERSION     DANKHJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>1508
                     /locus_tag="PW113_003480"
     CDS             <1..>1508
                     /locus_tag="PW113_003480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2527700.1"
                     /translation="KEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIHWIVALLDDEV
                     IPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKRQEMIVTQANE
                     LAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTSMKEHQRYFEV
                     RNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDKKASIDQYVEK
                     LKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIYKFDLVTNMVG
                     EFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSVLAIADKLDSI
                     FSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISDVISKDTDRFG
                     IGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFAAAQLFKQHLA
                     DEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEAMKKS"
BASE COUNT          503 a          270 c          335 g          400 t
ORIGIN      
        1 aaagaagtat tgacaggttt agcagatgtc attaaaagcc tcactttccc agtaacgatg
       61 cattgggcag aatacgattt tgaatatatc cgccctattc actggatcgt ggcattgtta
      121 gatgacgaag taatcccgtt tgagatctta gatgtcaaaa caggcagagc ctctcgagga
      181 catcgtttct taggcgaaga tgtgacattt gcgcatgcaa aagaatatga agaaaaattg
      241 aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag aaatgatcgt tacacaagca
      301 aatgaattag ccgaaaaaaa tcactgggtt attgatctag acgaagattt actagaagaa
      361 gtcaacaatt tagtcgaata tccaactgtc ttttctggcg acttcgaaga aaaatatctt
      421 tctgttcctg aagaagtact agtcacttct atgaaagaac atcaacgtta tttcgaggta
      481 cggaacgatc aaggtatgct gcttcctcat tttatttctg ctcgtaatgg tgatcgggta
      541 aaattggaaa atgtggttcg aggcaatcaa aaagtattga cggctcgttt agaagatgcg
      601 gaattctttt ataatgaaga caagaaagct tcaatcgacc aatacgttga aaaactaaaa
      661 actgttactt tccatgaaaa aatcggtagt atctatgaga aaatgcagcg ggtacaagtc
      721 attgcccgta tcattggcga acaagtagga ctatctgagg aagaattgat ggatctgcaa
      781 cgagctgctg aaatctataa atttgatcta gtaaccaaca tggtagggga atttcctgaa
      841 ctacaaggga tcatgggcga aaaatatgct ctgctgaaag gcgagaaacc tgctgtagca
      901 acagcgattc gtgagcatta catgcctata tccagtgaag gagagctacc tcagtctaca
      961 gtaggttctg ttttagcgat tgcagataaa ttagatagca ttttttcttt ctttgcagtt
     1021 gggatgattc caagcgggtc aaacgatcct tatgccttac gtcgccaagc atacggtgtc
     1081 gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt ctgttctgca agaaacgatt
     1141 agtgatgtta tcagtaaaga tacggatcgt ttcggcattg gtttatcaga agggcaacaa
     1201 caggtgatcg actttatcaa aggccgtctg cgccaattat tgacaacgaa gaatattcgt
     1261 catgatgtca tcgaagctgt tttgaatgca gaacaaaaag atctgacaaa agtatttgca
     1321 gcagcacaat tgttcaagca gcatcttgca gatgaagact tcaaaccatc gatggaagct
     1381 ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg aaaaacaatc agaagtagac
     1441 cctgaactgt tcgaaaatga agcggagaaa gaactgcaca aagcagtaga ggcaatgaaa
     1501 aaaagctt
//
LOCUS       DANKHJ010000276         1506 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000276 DANKHJ010000000
VERSION     DANKHJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(169..1095)
                     /gene="glsA"
                     /locus_tag="PW113_003481"
     CDS             complement(169..1095)
                     /gene="glsA"
                     /locus_tag="PW113_003481"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2527701.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1118..>1506)
                     /locus_tag="PW113_003482"
     CDS             complement(1118..>1506)
                     /locus_tag="PW113_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2527702.1"
                     /translation="VATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLK
                     LVFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETIL
                     IVSYLIVLVIPFTIYACRHKFQHAEK"
BASE COUNT          450 a          321 c          271 g          464 t
ORIGIN      
        1 aatcatatct gtaaatcgta tttttgtttc ctttttattt agaaaagccc tttcaaaaac
       61 caataaaaaa agaaactggg acaaaagtca aacaagctca caatttcagc ctcattgtag
      121 gtagctttga gccgtttgga cttttacccc agcctctttc aagaatggtt attcaaagat
      181 gtcagcgtct agttctttga ctacatcttt caacaattgg ataccagctg cgctgttacc
      241 aaatggatct agtgccgggc tgaagacacc tatgccgtaa cgatttggca cagctgccat
      301 caacccgccg cctacaccgc tttttgcagg tacaccaaca tggacggaaa actctcctga
      361 ttcatcatac aaaccagcag ttgtcataat tgatttgacg attgtagcac tttcttcagt
      421 aatgatttgt ttgccattcc aaggagcaat tcctttattt gccagcacag cagctaattt
      481 tgctatgcct gtagcggtga cattgaggga gcattgtctg aaataagcat cgagtatctc
      541 cggtacgtct ccttcgatca tttgattacc tttcatatag taagcaagag agcgattgat
      601 atcacctgtt cttgattcag aatgatagat ttcttcattc aatgtgatat taggatcatc
      661 acagatctct ttcataaatg acaagattcg ctcaaatttt tcatctgcat ctttaccggc
      721 aatcaacgag gttgttgcaa tcgctcctgc attaacaaaa ggattcattg gatgtttgcg
      781 atgattgatt tccatattca tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat
      841 tgtttgaaag actttttcta ttccgttgtc tttgattgct aataacaata cgggaacttt
      901 tgacatgctt tcgatggcaa agcgcacatc ggcgtctcct ccctcaatca gttgttttgt
      961 atccaaatca taaatcgcca tacctaattg tttgggatcc acttcagcta atgctggaat
     1021 gtaatccgcc acttgtcctg tttgataata gtggaaattt tttccaatca attgttctaa
     1081 ttcttttact tccatttgaa taaccagctt tcatttttta tttttctgca tgctggaatt
     1141 tatgacggca tgcgtagatc gtaaatggaa taactaagac gataagataa gaaacaatca
     1201 gaatcgtttc ataaatccca tgctgtccag ctgcgatact agcaggtgga acaaatgaga
     1261 tacaaaaagc aaaaagtgaa gtcaaaagac ctaaacaagc aacgattact tttacaactt
     1321 ttccgcctgg aacctgatag gctctttcaa gactgtcatc tttaaaaacc agcttcaaat
     1381 agcccgcgaa aaacataaag tacaacatca ggtagattac aacagtcaat gacatcgctg
     1441 caagaaatga aagattattc cccccgccgc ttaatgtaaa gacggctgcc cagattgttg
     1501 caacga
//
LOCUS       DANKHJ010000277         1499 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000277 DANKHJ010000000
VERSION     DANKHJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            219..849
                     /locus_tag="PW113_003483"
                     /pseudo
     CDS             219..849
                     /locus_tag="PW113_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            987..>1499
                     /locus_tag="PW113_003484"
     CDS             987..>1499
                     /locus_tag="PW113_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HDL2527703.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSI"
BASE COUNT          580 a          268 c          268 g          383 t
ORIGIN      
        1 tgttactttt cactgcttgt ggtaaaaaag taactacaga agatttaaaa gctaatgatt
       61 ggttgattga agcaaccaaa gatgacgaac ctaatatgat cgtatcattt tctgatcacg
      121 tcgtatcttt tagtgttgac acaagttcta tgaaatcaac ttcaagtaac gattgggagg
      181 ctatgggaga agaatttgct aaaagcttag tcgaccaaat gaattacaaa tttgagtata
      241 cacttgaagg cgatgttatg acttggaaag atgataaaga caaagataat gatgccaaat
      301 atactgtcag caaagaagat aaaaatatta ttttaactcc agaaaagcct aacaaatctg
      361 atgataatga gaaattagtt ttgaaaccct acaaaaagga aaaaaactaa agaatctaga
      421 acaactacta gctcaacaag tagcaacgaa caatcatctt cgactacaac agaaagtact
      481 attgcatctt ctagtactgt agaacaagct gctattgcgc agaatacgcc tcctgaaact
      541 agtagtactt actctgctga aagtgtagaa aactatgcag aagctactcc tagttcaagt
      601 gttgaacaag tttcacccac tcccagttca acggagcaat cagtaccaca agattatgta
      661 ccagctaatc aagctcaacc aacggcacct tcttcatctg aaaccgaaac cttgactttg
      721 caacctggtg aagggaccaa acaaatagct gaacgagctg gaattacggt agatcaactg
      781 tttgcattaa acggtatgga tccaaacaac tacatgcttt atccaggaca agaactaaga
      841 gtgaaataaa aaaagcacat ttgccgcccg accaagaacg caaatgtgca aagagctacg
      901 gtaaagtagg tctatttgtt gtaccctatt ttaccatctc gaaatccgct gtacaaccga
      961 acaaacgtac gaaaggaaag ataaagatgg caaaaaaaga aattgataaa cgaattaaaa
     1021 agcacttaac aaaaaaaggc gaagaaaaat atcagtttag tctgtactta ggagttgatc
     1081 ctctaacagg aaaaaaaaga aaaacaacga gacgaggttt tagtacgcca ttagccgctg
     1141 agagagcttt aaaaagattg gaggcagata tacaggaaaa aggactgcaa gcttctacag
     1201 ctcaaaagag caagaaattc gaatatgttt acggcttatg gtttgaaaat tataaaaaga
     1261 cagttaaaga aagcacttgg tcctccacca aacaaatttt tgacacacac atcttaaaag
     1321 tgtttggaga taagttcatt gacaaaatag acgttttttt ctgtcaagaa gcagtaaaca
     1381 cttggtccga tagccatcct aaaattttta agaaaataaa aaattacaca tctaatgttt
     1441 ttgattacgc tgcttcttta caaattataa ctagcaatcc tatgaaatta gtttctatt
//
LOCUS       DANKHJ010000278         1487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000278 DANKHJ010000000
VERSION     DANKHJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..506
                     /locus_tag="PW113_003485"
     CDS             <1..506
                     /locus_tag="PW113_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2527704.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
     gene            568..1185
                     /locus_tag="PW113_003486"
     CDS             568..1185
                     /locus_tag="PW113_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527705.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          539 a          221 c          275 g          452 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggaataaca gcctaagata tttggagttc agagagggcg cgtttgattt
      541 tcaaacttcg caacagaacc gagaaccttg ggttttcaag tgctttttgg ttcattaatc
      601 ttaaccatag tgtttttaaa gtggaaaaca agcaacaaaa aaagtatgtg gagccttgta
      661 ggaatagttc ttattatttt tactgtgact gcagcaacag aagcaaatta ttcgcataaa
      721 attcagtcgt ataaaggtcg tttagttatg accaaaaaag atgttacacg ttatacatcc
      781 tcaagaactg gaggcctata tgactatgca ccattagatg cattgaaata taaagatcat
      841 ttaaagaaac atgaagtaaa agttggtgat cggtgggaga atgcatctca taaagcatat
      901 aattccaaaa tagtctatac agtagaagat gccgatgagc aaaatatcac attacctgta
      961 ttcaattatt ggggaacagt tgtgaaagta aatgggaaaa agagcactat agaaaatgac
     1021 aatggtttag tgtctattaa aggtacgggg aaaagaacta tagttgaggt ttcatctacg
     1081 tatccaacta cgatgattct atctttatta attagttcaa taacttttat attcttattt
     1141 acacgttatt taataagatg ctgcggtgaa atgaaaatag attagataga tattataaat
     1201 atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata aaagaaggta
     1261 tcaagagtca cagtgaattt attccgaaaa gtgaaaagag caaatataaa atttaatgaa
     1321 aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt ttcatatata
     1381 ttaaatatta cggaaaatta tataaactaa tttagtgttt tgtttgtaat tcctaatttt
     1441 gctccacttt tagataattt ttaggtctat ttaactcatc agacatt
//
LOCUS       DANKHJ010000279         1483 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000279 DANKHJ010000000
VERSION     DANKHJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.291"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            64..972
                     /locus_tag="PW113_003487"
     CDS             64..972
                     /locus_tag="PW113_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2527706.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="PW113_003488"
     CDS             972..>1483
                     /locus_tag="PW113_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527707.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DANKHJ010000280         1480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000280 DANKHJ010000000
VERSION     DANKHJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            64..972
                     /locus_tag="PW113_003489"
     CDS             64..972
                     /locus_tag="PW113_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL2527708.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="PW113_003490"
     CDS             972..>1480
                     /locus_tag="PW113_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527709.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DANKHJ010000281         1479 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000281 DANKHJ010000000
VERSION     DANKHJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..474
                     /locus_tag="PW113_003491"
     CDS             <1..474
                     /locus_tag="PW113_003491"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="catalyzes the formation of alpha-D-glucose
                     1-phosphate and UDP-galactose from UDP-glucose and
                     alpha-D-galactose 1-phosphate in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2527710.1"
                     /translation="TVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHP
                     DGIFHPHKDVQHIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPW
                     AEQLKKEHPDLTKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            498..1478
                     /locus_tag="PW113_003492"
     CDS             498..1478
                     /locus_tag="PW113_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2527711.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT          500 a          268 c          323 g          388 t
ORIGIN      
        1 acagtatcta tcagagcttt ttcagcagat ggtacaccac accatacgat tacgccaatt
       61 gcacgaagaa gggacaatta ctttgagttg gaccttgtcc ttcgagacaa caatgtttca
      121 gaagaacatc ctgatgggat ttttcatcca cataaagatg tccaacatat caaaaaggag
      181 aacattgggt tgatcgaagt aatggggctt gctgttttac cacctcgttt gaaaccagaa
      241 ctagtagaag tagagcattt cttattagga gaagaaaacc agatggctga atatcatcgt
      301 ccgtgggcag aacaattaaa aaaagaacat cctgatttga cgaaagatac ggtcaccgat
      361 gtcgtacaaa aatctgtagg tcaggttttt gcacgtgtac tggaagacgc tggagtattc
      421 aagcgagatg aaaaaggaca ggcagcgttt gaacgattta ctgcaatttt ataaacgtaa
      481 aaaaggagga gagacaaatg gcaacaatta aagacattgc gcagttggca ggagtttctc
      541 ctgctactgt ttcaagagtt ctcaactatg atcctgatct ttccgttggc ttagccacaa
      601 agcaaaaaat ttttgaagca gcagaacgct taaattatac gaaacatcgt aaaaatgcag
      661 caaatactaa gcaaacgctt cgcctgatcc agtggtacga cgatacggaa gagcttgaag
      721 atctttatta tttatctatt cgtctaggaa tcgaaaaaaa ggcagaagaa gcaaacgtta
      781 ctttattgaa agaaacgtgg gattccatta gtgatcttcc gacagatgga acgatcgcac
      841 taggaaaatt cgacgaagct cagttattag aaatgaaagg atcccaaaat gccttgcttt
      901 tggtagattc tgacggcact tctcaaggaa tcgactctct agttgtcgac tttaagtcaa
      961 gtgttcaaaa agtcattgaa cattttgtct cccataaagc gcaacatatc gctatgcttg
     1021 ctggaacaga atatacgaag aaaaaccatc agcggctaaa agatcccaga attatggcat
     1081 ttcaacaagt tttggcagaa aaaggctacg acgaaggtcc aattatcgaa gcagattttt
     1141 ctgtcgagtc aggatatcaa gcagtgaagc agtatttaaa aacgaatccg catgttcctg
     1201 atgcgctttt tgcagctaat gatgcattag cgattggcgc actgaaagcg attcaagaaa
     1261 aaggtttagt ggtaccagaa gatatttctg tgatgggttt caatgatgtc agtgtagcga
     1321 aatatgtaac gccctctctt tctactgtaa aagtttatac cgaatggatg ggagagctag
     1381 cagtggagac gattcttcaa ttaagtaatt ccaaagcacc ggtttcaaga aagattattg
     1441 tggaaacaga gttgattatt cgagaatcaa cgaaataaa
//
LOCUS       DANKHJ010000282         1477 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000282 DANKHJ010000000
VERSION     DANKHJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..277)
                     /locus_tag="PW113_003493"
     CDS             complement(<1..277)
                     /locus_tag="PW113_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2527712.1"
                     /translation="MESLASTMEKLIRRVLVQSGRCPECSEPLYSWRGKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFK"
     gene            complement(294..1124)
                     /locus_tag="PW113_003494"
     CDS             complement(294..1124)
                     /locus_tag="PW113_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2527713.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDIDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(1127..>1477)
                     /locus_tag="PW113_003495"
     CDS             complement(1127..>1477)
                     /locus_tag="PW113_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527714.1"
                     /translation="NVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCI
                     CGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGI
                     KLNQETIKKLRIGG"
BASE COUNT          360 a          314 c          217 g          586 t
ORIGIN      
        1 atttaaacaa agttttatct gttaataccg aaccattttg aaaaaaactc aacgctcgtg
       61 cttttaagct gtcgttatat atccgttcgg tctgtatatc ctctttcaca cgtaacgctt
      121 tataaccaca actcatgcat gttggtttac aacgttctga cccgtcctta tttttccctc
      181 gccaactata caaaggttcg ctgcactctg ggcatcttcc actttgtact aaaactcttc
      241 ttattagctt ttccatagta cttgctaggc tttccatgtg atgcatctcc tttttaaatt
      301 ggcaagtcgt catattcact aggattgctg tactgtaatt tttgactttg ctttttatga
      361 ttatttttat ctgccttgat ttcgaattta agcttctcaa atttttctcg cagtttctta
      421 gcacttctaa tatttccaaa ccaaaattca tttgtaggta gccaattgat cacatactca
      481 atcgcttcta tagatgcttt atctctttct tccatcaacc tgattgtgtc tgcccatttt
      541 tcgatatcta ctttattcat ttcttttgga aaatcttcag ttaaattact ttgcaacttt
      601 ttagcaaggc gtaagtgttc gtcagaatac ttacctttct tttcttcttt atctatatct
      661 atatcttctt ctatatcttt atctgtaccg tcacgtgacg tcacgctaac gtcattttcc
      721 aatttgagac gttcctgtct ctttctttct ctgtatttac ggtttctttc agcgtttttt
      781 agccttactt tatccatccc ttcgatattt tgatgttttt cccaattact gatggcaatc
      841 agtccatcac tgcctagatc aatcatgttg aaatttgcca atgtagttag cgctaggcga
      901 accgtattta cgtttttgcc gaacaatgta gcaagcattt cttcggtata aggcatgttc
      961 ctctggatat atatcagacc atcgtcgtta gtctttcctg ctaaaactag taatcgaatc
     1021 catataacga tgatggcatc cgactcagga acagcttgga ttaatcgtat tttttcatcg
     1081 tcaaacatag tagttttaag tttgatccaa cttatctcag ccaaatttat cctcctatcc
     1141 ttaacttttt aattgtttcc tggtttaact tgattccttt gatttgatat ttatttttga
     1201 aattgatcac acctattttg tgtttctctg tgtgatggat tctgcagagt gctgcaaatg
     1261 tgtactctga atgatcaact tccttgcgct ttcgtcttcc cagcgctttg tcaaagtgat
     1321 cgatatcagc tcctgttttg ccacagatgc agcagactct ttttgtaatg catttgtaga
     1381 agtaatactc ttgattcgct ggtaaaatct catatccttc tttgaaagga atatgatgtt
     1441 caaagatgaa atctaaaatg atatttgcta agacatt
//
LOCUS       DANKHJ010000283         1451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000283 DANKHJ010000000
VERSION     DANKHJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            51..1391
                     /locus_tag="PW113_003496"
     CDS             51..1391
                     /locus_tag="PW113_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2527715.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          459 a          320 c          285 g          387 t
ORIGIN      
        1 aaatccttta gaataaagtc gacgaaaacc attcaaagga gtgtttcaag atgtcctatt
       61 cagagtttac caaagagtta ctagatattc tagacctaaa tcttaccttt catgaagatg
      121 cctttcgcaa agagcgcatt aatgacgaaa catgttttgt ttttgatggc acattgacct
      181 atcaaccaga ggaatgcttc cattgccact atcaaaataa gcaaacgatc attaaatggg
      241 gctggaaaaa agtatccatc ttattgaatg atgtcagtaa ctacaagacc attgtgcgga
      301 ttaacaaaca gcgcttcaaa tgtaaacact gtggaaagac ttttttagca gaagattcag
      361 ttagcgatcg tcgctgctcc attgcccgca gagtcaaaca agccattctt gagctattaa
      421 gtgagcctat ttctatgtct ttgattgcac gtatgaagca catttctccg accactgtca
      481 ttcgaatact tcgcagcctt cggcctaaaa ccgtttcttt gaaccagccg ttaccagaag
      541 tggtgtgttt cgatgaattc aaatcggtga aaaacgtctc gggagccatg agctttgtca
      601 tgatggacgg caagacccat caactgattg atattgtgga aaatcggcaa ctcaatcctt
      661 tacgcgacta tttcttgcgc taccctagaa aagtccgtga acaagtacgt ctagtggttt
      721 cagatttcta tacaccctat cgcacattgg tgaaagagtg ttttccgaat gcccgaatcg
      781 tcgccgatcg ctttcatatt agccaacaca tcggtcgggc ctttaccaac caccgaattc
      841 aagtaatgaa gacctttaaa aaaggggatc gtcggtcgaa gcacttaaag aaatactgga
      901 gactacttca aaagaatgcg tgggaattga atggtcagca tcgctactgg cgtccgtcat
      961 ttcgagatca tctgacagaa gcggagatag tcgatcggct attatattat gatgactcct
     1021 taaagcgagg ctacgaagtc taccaggttt ttctttccgc tatcagaaga caagatgttc
     1081 ctgaattcgt cgcgctccta aaagaagact acaaggagtt gccagagcac tatcaaccag
     1141 tgttcacgac cttcaagaaa tatcgaacag aaatcaaacg agcgttgcga gttccctatt
     1201 ccaacggccc tatagaatgt ttgaacaacc atatcaaagt actaaaacga attgcctatg
     1261 gctttagaaa ctttcaaaac taccgagaac gaatcttttt atatcgagga aaatatttca
     1321 agaaaactaa gaacactacc caactgacta aagccagaac aacaacacgg ctagacaaga
     1381 tagctgtcta gccgtgtgag tatctcgatt attctattga atttcttcac caacactcat
     1441 tgacgaagag c
//
LOCUS       DANKHJ010000284         1442 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000284 DANKHJ010000000
VERSION     DANKHJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            81..1030
                     /locus_tag="PW113_003497"
                     /pseudo
     CDS             81..1030
                     /locus_tag="PW113_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1075..1442)
                     /locus_tag="PW113_003498"
                     /pseudo
     CDS             complement(1075..1442)
                     /locus_tag="PW113_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
BASE COUNT          463 a          254 c          261 g          464 t
ORIGIN      
        1 agaggacagc atatcgatat tgcttccttt tttttgctat ttatttaagg gatagaagtt
       61 tagaaaggac tgtttataag atgaaaaatg atatagtgac ttatgcaaac acaaaatgtg
      121 aagaaattgg catcagtaat ttagaagagt gcttttttga cacaaatgac ctgtcgcaat
      181 ttttaagaac gggaagaaca aatgccagta aacttgccaa tgacttagtt gcaaagaacg
      241 ttctatttaa aatcaatact cgtccagtgt tatttgccat caaagagcgc tttgaagagc
      301 actttttgat tccggtaaaa gatgtatacg aaagtatatc tgaattaaaa acagacttgg
      361 agcccaattc tacagattcc attctacaaa agatgattgg gtatgatatg agcttatccg
      421 atgcggtgga acaaatgaaa actgctgtct tctatcctgg aagagggctt cctatgatga
      481 tcactggaga tactggagtt ggaaaaagtt tttttgttca tactgcttat cagtttatga
      541 aaaaagtgaa cgttattcat gagaatgctc cttttaaagt acttaattgt gcgcaatacc
      601 ataataatcc tgaattattg tcggggatac tatttggcta tgttaaaggt gcttttaccg
      661 gcgcagatga agatcgaaat ggattgttgc aagatgcaga cggtggagta ctgtttttag
      721 acgaagtaca cagattgcct cctgaagggc aagagaaact atttgtttat atggatggcg
      781 gaacttattc tagagtcggt gaaactatcc atcacgcctc caatgtacgt ttagtttttg
      841 cgactacaga aggcaaagag gattttctgg aaacctttat gcgaagaatc cctatacata
      901 tccatatccc aaatattgag gaacgaggta ttttagaaaa aaacaaatta tcgaaaatac
      961 tctctttagc gaaagcaaaa actttagcaa ggctattgat gtttctccta aagtcactag
     1021 tgttgcataa atatagtcag aatattctga attattattt tcttgaaaaa agtaaaaaaa
     1081 atcgtatccc aaactttcat ctacatttcc agaaagatct tcatgttgat aatcaattcc
     1141 tgaatcaatg accgctacct tcacatcctt agaacccttg gtttcttccc aagcggatgg
     1201 catgctaatc tctcttaaat tagcttgttt actccaatta ggatcgttat cgatttccta
     1261 aagaatcagt tgtatttggc tcctcaacat cattcaacgg ctctagttca accacatagt
     1321 ttggctctgc ataattgacc atatcagaag cattaaatgt tcttactgcc tcttccacac
     1381 taatttcttc gttcaatttc actaataata cttgatctcg cttattataa tcgctttcat
     1441 tt
//
LOCUS       DANKHJ010000285         1440 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000285 DANKHJ010000000
VERSION     DANKHJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            474..725
                     /locus_tag="PW113_003499"
     CDS             474..725
                     /locus_tag="PW113_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527716.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="PW113_003500"
     CDS             759..1037
                     /locus_tag="PW113_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527717.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          513 a          197 c          267 g          463 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
//
LOCUS       DANKHJ010000286         1390 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000286 DANKHJ010000000
VERSION     DANKHJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(1..144)
                     /locus_tag="PW113_003501"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="PW113_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="PW113_003502"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="PW113_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2527718.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          397 a          251 c          232 g          510 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc
//
LOCUS       DANKHJ010000287         1372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000287 DANKHJ010000000
VERSION     DANKHJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            121..804
                     /locus_tag="PW113_003503"
     CDS             121..804
                     /locus_tag="PW113_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527719.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(841..1338)
                     /locus_tag="PW113_003504"
     CDS             complement(841..1338)
                     /locus_tag="PW113_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2527720.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          432 a          239 c          231 g          470 t
ORIGIN      
        1 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
       61 atagtagtct caaaactccc aagaaatgag gactatttaa caaactacga tggttacgtc
      121 gtgacaactg aacattttga cttagccaat cctgttacta aagtcgatga tatccccgat
      181 tatgaaatgt acagtcaaac aatcgatagc ttaaataagc gttttggcaa tcgtgtttta
      241 aaaggcatca aaatcggtta catcgcttct gaaaaggatc ggatcatcga ctatttagcc
      301 gataaagatt acgatttaaa attactcagt gtgcatcata atgggcaatt tgattattta
      361 gatgacgaag tcaaagatat ggatcctgct attgtgattc ctcagtattt tgcacaactt
      421 tcagaagcac tagttgttat agaagcagat gtctttgctc attttgacta tggttttcgg
      481 gtatttggtc tatccgttgc tgaatttaaa caatatgaag cgcagttttt accgattcta
      541 gatcaagtaa tcaaaaataa attggcattc gaattaaatg ctaagagcgc ttacttgtat
      601 gacaatttag cgttatatga atatgtcatc gatttatatc tttcacgtgg tggtaccttg
      661 tttagtgttg gatcagatgg gcattacctt gaacatttcc gtttccattt cgatgatctt
      721 tttgctctgt taaaagcaaa tggtgtgacg gaattagcga tttatcaaaa aggaaagcga
      781 attatggttc cattaccagt ctaaaaaaac gtcctcagca tcaaaagtga ggacgttttt
      841 ttagatttct ttttctaaaa tcggacaaat acgatcttgt tttcctaaag gaaaatgttt
      901 gatcccgatt tttttaaatc catgacgttc ataaaaacga atacctgcat gatttttttc
      961 ataaacagtc aaacgtaaat aacgtttttg tttcgtttta gcataacgat tcgcttcatc
     1021 gagtaaccgt tgccctaacc ctttgttttg ataatctttg agcagataga gccgttgaat
     1081 ctctatagca tctttttcat cattgacttt catataacca atgagtattt gatgatcctt
     1141 aataccaata aatttactgg ttgactgtgc caattctact gccaatattt cagttgtcaa
     1201 ttgtgtctgt aaatatcctt gcatatcaag tggatctgcg ccatcgataa acgattcata
     1261 aaaactgaca acacttacaa cttgtaaagc atctagatca tcataatcta aatacgctag
     1321 tgtgatttga ttgttcatct aactcctcct tttgacatag tatactataa at
//
LOCUS       DANKHJ010000288         1369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000288 DANKHJ010000000
VERSION     DANKHJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..651
                     /locus_tag="PW113_003505"
     CDS             <1..651
                     /locus_tag="PW113_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComFA"
                     /protein_id="HDL2527721.1"
                     /translation="GLAHAVQTAIRSNGRFIYLSATPDEKLLKEIKQTFELHKLALRF
                     HRRLLPEPVLVFMNNWRQKCGNKKKINSLVRIIQQLIKENRILLFCPSITMMHRLKIT
                     LQEAFPEYEITDVSSQDDQRAKKVQKMREAIYEILLTTMILERGVTFERISVIVLGAD
                     HPVFTKSSLVQIAGRADRKGPYTNSRVYFFYEEKTSAIRKACQEIKEMNEEGKQML"
     gene            648..1367
                     /locus_tag="PW113_003506"
     CDS             648..1367
                     /locus_tag="PW113_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2527722.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
BASE COUNT          474 a          264 c          278 g          353 t
ORIGIN      
        1 gggctagctc acgctgtgca aacagcaatc agatcaaacg gccgattcat ctacttaagt
       61 gccacgcctg atgagaagtt actaaaggaa atcaagcaga catttgagct ccacaaactt
      121 gccttgcgct ttcatcggcg cttgcttcct gaacccgtgc ttgttttcat gaataattgg
      181 cgacaaaaat gtggaaacaa aaagaaaatc aactctttgg tgcggatcat tcagcaacta
      241 atcaaagaaa accgtatcct tttattttgt ccaagtatta cgatgatgca tcgtttaaaa
      301 atcacactgc aagaagcttt tccagaatat gagataacag atgtttcttc acaagacgat
      361 caacgggcaa agaaagttca gaaaatgcga gaagcaatat acgagatact gttaaccaca
      421 atgatcctag aaagaggagt cacatttgaa agaatctctg tcatcgtttt aggagcagat
      481 catccagttt ttactaaatc ctctttggtg caaatcgcag gaagagctga tcgtaaaggt
      541 ccttatacga acagtcgagt ctattttttc tatgaagaaa aaacttcagc tattcgaaaa
      601 gcctgtcagg aaatcaaaga aatgaacgaa gaagggaagc agatgctgtg agatgtagtt
      661 gctgcggtaa agacatcatt caaaatttaa cattcagcga gatcatcgga gtgtcgaaat
      721 ctagacttcc tcagacatgt tcagtttgcc gcgcacaatt ccatttatta gaagcaaaag
      781 gttgtgcagg ttgtcaaaaa ccaatccatt tgaaatacga ggagacgaaa aagaaagcaa
      841 atactagttt cgtttactgc aacgactgtc aagaatggaa gaagaaatat cctgcatatg
      901 attttcaaca ccgcgccttt tttacgtacg atgaaaagat gcaggcttgg atcaatcggt
      961 acaagtttat gggagatatc cgattagcag ggacttttgc atataaatgg actcagataa
     1021 aaaaagatta cccggcatat atttattgtc caataccttt atctgaaaaa aggtggctag
     1081 aaagaggatt caatcaagta agcgagatgt tgaaagcagc aaaaattcct tttcagatgc
     1141 tgctcaaaag agagcaacat ctgcttgctc aagcaaaaaa cacgcgaaag gaaagattgg
     1201 agatgcctca gccttttgag cttgccgtac ctctttctaa gatacaacat aaaaaaatat
     1261 tgattattga cgatgtctat acaacaggac agacgctttt tcatgcagca gattgcttgc
     1321 agaacgcaca gccgaaaatc atccgaacca tttctttggc aagatgaat
//
LOCUS       DANKHJ010000289         1364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000289 DANKHJ010000000
VERSION     DANKHJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(140..1288)
                     /locus_tag="PW113_003507"
     CDS             complement(140..1288)
                     /locus_tag="PW113_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527723.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DANKHJ010000290         1362 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000290 DANKHJ010000000
VERSION     DANKHJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            82..>1362
                     /locus_tag="PW113_003508"
     CDS             82..>1362
                     /locus_tag="PW113_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2527724.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DANKHJ010000291         1333 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000291 DANKHJ010000000
VERSION     DANKHJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.304"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..705)
                     /locus_tag="PW113_003509"
     CDS             complement(<1..705)
                     /locus_tag="PW113_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2527725.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRL"
     gene            complement(894..>1333)
                     /locus_tag="PW113_003510"
     CDS             complement(894..>1333)
                     /locus_tag="PW113_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2527726.1"
                     /translation="PGMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLIT
                     ENGLGEYDKLTEDKQIHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLN
                     GYQKRYGFVYVDQDETQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT          360 a          299 c          216 g          458 t
ORIGIN      
        1 taagcgcctt gcttctttcg cttctgcgcg ctgtttcatt ctctcttcct ttgccgcttg
       61 ctttcgagct tgcttggcag gatcaccttc caataattcc tgtactcgtt ctccaattga
      121 ttggatacca tttaacagct gatagccttc cagattactg aacaggaatg ctcctcctgc
      181 cagtaatagc actgctgcta aataacttcc aggctgtgca atcaagaagt aagtaaaatg
      241 ataaaggatt gctccaatca tcccaccacc aacgttattg tctacttgac tttgttttat
      301 atcactggct aacaaatccc atgtcgtccc catgatattt ggttccccag tatggagttt
      361 gccaaataaa tgggcatgta acaataataa gataccgaaa taaaagagta tgccgccaaa
      421 ccagcgtcgg tttttaccaa ttgaaaactc tgtattctta atgacaatcc aaaatcctaa
      481 tacggctaat aaaagacaga ggatttggta agtatttcct gcaatgatcc gaaagccgtt
      541 tgcaatcaat attcctaaaa aacctaattt taaaaagccg aagacagcaa ataaaatcaa
      601 ggcaaaccca acaccaatcg tcagcatttg ttcctgctgc tgtttttgct ttttcgtttt
      661 tttcgtcttt ttcctttttt tagtaggccg tttcttttgt gccatttatt atccctcatt
      721 tcatcaagtt tcattataca aaaaaaagta agggaactaa agttttgttt gaaagaactg
      781 acgctttctt tcatttttca gcttatttta cagtttttct taatttctaa atgattttat
      841 caagaaaagg cacggccata tccagccgta ccttcttgtt ttaagtatgt gaactaaagg
      901 ttctctccat tttcttcaat cagtttttgg taccagtaat aactgtcttt cttatatcgt
      961 tccaaagatc cttcttgtgt ttcgtcttga tcaacatata caaatccata tcgcttttga
     1021 taaccgttca gccagctaag caaatctgta taagaccagg tgcaatagcc taggacttct
     1081 gctccatccg aaattgcttc tttgatggct ttgacgtggc tttgtaagta attgattcga
     1141 tagtcgtcat ggatttgttt atcttctgtc aacttgtcat actctcctaa gccattttcc
     1201 gtaatcagta ccggcatgcg gtaacggctt gtgatacgac gcagtccgat ccttaatcct
     1261 tctgggtcaa tctcccagtc ccaatttgtt cgttcgacaa atggattctc aactttttta
     1321 tacattccag gaa
//
LOCUS       DANKHJ010000292         1325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000292 DANKHJ010000000
VERSION     DANKHJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            181..603
                     /locus_tag="PW113_003511"
     CDS             181..603
                     /locus_tag="PW113_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527727.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            620..1042
                     /locus_tag="PW113_003512"
     CDS             620..1042
                     /locus_tag="PW113_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2527728.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            1071..1271
                     /locus_tag="PW113_003513"
     CDS             1071..1271
                     /locus_tag="PW113_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527729.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
BASE COUNT          522 a          191 c          234 g          378 t
ORIGIN      
        1 tataacaaaa ccttttattt gcacttggtc gccaaacttt atgctataag gtgtgttttt
       61 agtttgcttt tttctatcaa attaacttac ggataaataa taactctata tcgtaaaatt
      121 gtaaagctat ttttactctt tttcgtaaat tattttttcg tgttggaaag gaacgttgga
      181 atgacaacat ttgaaagaat aaaagaacta gcgaaaaaca agaataaaaa ccttcaacaa
      241 gtcgctaaag atttgaagta cggagagaat tacttttata gtttaaacgc aggaaaacag
      301 ccaactgccg aaaagttaca agaaattgct gactacttcg acgtatctgt agattatttg
      361 ctaggacgta ctgagaaaaa aagatactat gatttaacag aaaaagacga gcgtgatatc
      421 caaaaagaat tgcagtcatt aatcgacgat ttatctaatg ctgatggaat ggctttttcg
      481 aagaaagata atgaaatgag tgaagcgaca cgtgaagctc tcattatttc tttagagaat
      541 gctttgcgaa tagctaaaat agaagctaag aaaaaataca cacctaaaaa atatcgcgat
      601 taaaaggagt acgttgttta tgtatttacc tcaaattgat aacaaaatta ataagctggt
      661 aaaactatat caaacacgca atccttttag aattgcacga gaattgaaga tactagtttt
      721 agaagaagat ttgggagaaa tttatggtta ctatagtaaa ttcaaacaga taaaaatgat
      781 ccacataaat tctagtttag atgagatggg aaaatggata acttgtagtc atgaattagg
      841 ccactgtgtt ttgcatccga atgaaaacac acctatgttg tctaaaaaaa cgctagtatc
      901 tgaattaaaa attgaaaaag aagcaaatta tttcgcaact catctgacta ttgatccaac
      961 tttagacggc tttgatcata tgacaaaata tgaaaagctc gtctgctttg gattaccaga
     1021 tgaattcgat cgtttcttgt gaattataat acaacgtaag gaggatatag atgaaaatag
     1081 gaatgagaaa accaagtata aaaaaatcca taagcgctag aaccactgga aaagcaaaaa
     1141 gaaaagtaaa aaaagcagtt attcctggtt atgggaaaaa aggtagcggc tggattaaag
     1201 acccaaaaaa ggctgcttac aataaagtat acaagaaaac tacattcagt ttttgggatt
     1261 tatttaaata aaaaaagccc gtgcagcaac acggactcga aaccttattt ctaagattcc
     1321 ccaat
//
LOCUS       DANKHJ010000293         1310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000293 DANKHJ010000000
VERSION     DANKHJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            128..>1310
                     /locus_tag="PW113_003514"
     CDS             128..>1310
                     /locus_tag="PW113_003514"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2527730.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
BASE COUNT          411 a          262 c          235 g          402 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaatcgt
      301 taaaaatggc aagaagatct ctatgattct cctgaatcgt agcggtaata aacggaccta
      361 tcttcggttg aagaaacagc gatacctctg ccgagcatgt aaaaaatatt ttaccgctcg
      421 gacgtatctc gttaccccat tctgctttat ttctaagcag attcattaca agatcttaga
      481 agaattgaca gagcgtcaat ctataaaagc aattggtaaa cattgcgatg tttctgtgac
      541 aactgttcag cggacattgt ccgccttgtc atcagatatc cgtcagaatc ttgagtattt
      601 acctcggtgt ctattattcg atgagtttcg ttctctttct acatggcacg gtaaattttc
      661 tttttcttgt atggacggtg agaccgggaa actgtttgag atcttaccta gccgccgtaa
      721 aaaagatctc gttgcttttt tcatgcgttt ttctctcaaa gcgaggttgg gtgtacatta
      781 catcgtaacg gatatgaacg ctccttattt ctcactagtt aaagagtgtt ttccaaatgc
      841 acaagtgatc gtcgatcggt ttcacattgt tcaacatctg aaccgcttct tcgatgcggt
      901 tcgaaaacgt gtgatgaaaa cacttgatca aaaagaccca atacaagcta aacagtatcg
      961 ccaattgaaa tccttattca aattactttt aaaatcagag gatcgtttag attacaatac
     1021 actaacgaaa agacgcaatt tcaattggag actattgact gaaacagaag tagttgaccg
     1081 ccttctaaag atttctcctg aacttaaaac agcttatcgt tactaccaag acctgcttac
     1141 tgcgtatcag gaaaaagatc cagatacttt tttccagctg cttcgagcaa tgcctggaga
     1201 agttccattg gaactgcacc atatcaaaaa agcctttttc aaatacgaga aagggattcg
     1261 gctggcatta ctgaagaagt attcgaatgc acgactggaa aatctccata
//
LOCUS       DANKHJ010000294         1295 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000294 DANKHJ010000000
VERSION     DANKHJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            143..400
                     /locus_tag="PW113_003515"
     CDS             143..400
                     /locus_tag="PW113_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527731.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="PW113_003516"
     CDS             complement(632..1063)
                     /locus_tag="PW113_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL2527732.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          488 a          165 c          162 g          480 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccct
//
LOCUS       DANKHJ010000295         1266 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000295 DANKHJ010000000
VERSION     DANKHJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..402
                     /locus_tag="PW113_003517"
     CDS             <1..402
                     /locus_tag="PW113_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2527733.1"
                     /translation="KGYKEALSENNLPYSEGLIFEAQYNFKDGLSLVDRIHNSGATAV
                     YVSDDELAIGILDGLLDRGVKVPEEFEIITSNNSLLTEVARPRLTSITQPLYDLGAVS
                     MRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(739..>1266)
                     /locus_tag="PW113_003518"
     CDS             complement(739..>1266)
                     /locus_tag="PW113_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2527734.1"
                     /translation="TTPFKDAISSLNGNLGKADWVGKTGSTNEYRDSWLIVSTPSITI
                     SSWAGHDDNTGMDSKARIRSANYLANLINQAYQAKPDIFGTDEKFELSSDVMKKKVSG
                     FTGQTPGKVTVNKKSIQTPGKTVESLWAKNGPKKSAFKFGVGGTDENYTDYWNTASAY
                     ARANQATKESDEKDD"
BASE COUNT          380 a          232 c          251 g          403 t
ORIGIN      
        1 aaaggctata aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt
       61 gaagcacaat acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt
      121 gcaacagctg tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta
      181 gaccgtggcg tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg
      241 acagaagtag ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg
      301 gtatctatgc gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt
      361 atcttgcctt atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca
      421 gaggttgtgt gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc
      481 tcatgtttga tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt
      541 gggaaaaggg gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga
      601 gaaagtggtt gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt
      661 tgttcaccac catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt
      721 actgtgtatt ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc
      781 tcttgcataa gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac
      841 gccaaattta aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt
      901 tcctggtgtt tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa
      961 tccagatact tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa
     1021 aatgtcaggt tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg
     1081 tatccttgct ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt
     1141 aatagacgga gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt
     1201 tccgacccaa tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg
     1261 tgtagt
//
LOCUS       DANKHJ010000296         1259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000296 DANKHJ010000000
VERSION     DANKHJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.309"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..579
                     /locus_tag="PW113_003519"
     CDS             <1..579
                     /locus_tag="PW113_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2527735.1"
                     /translation="NVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRV
                     GPEIFVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSV
                     TIENLGLAGSMNSFARNLGMVIGISLSTTVLYQAMSAKMGQRVTNYIADRPDVFLYGM
                     KITFLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            924..1091
                     /locus_tag="PW113_003520"
     CDS             924..1091
                     /locus_tag="PW113_003520"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527736.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
BASE COUNT          352 a          228 c          282 g          397 t
ORIGIN      
        1 aatgtagtga ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgccgga
       61 ctgttgatga tggtctttcc cttgatgatg gtcgtcgggg cgcctttaag cggatacctg
      121 accgaccggg ttggcccgga gatttttgta ttagtaggct tgggtttatt gtcggtaacc
      181 caagtcatgt atatgttttt gtcagtggac tctcctttgt ggctgtacat tgtcgctact
      241 gccatcatgg gtgccggcaa tgccttgttc cagtccccaa ataatacgat ggttatgagt
      301 tctgtcacta tcgaaaattt gggactggca ggctccatga attcctttgc ccgaaatttg
      361 gggatggtca tcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag
      421 atgggccaac gagtaactaa ctatatcgca gatcggccgg atgtcttttt atatggaatg
      481 aagattacct ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg
      541 cgtatcaaaa aacaaggagg tattcagaaa aatgtctgaa aaaccattgt tgaagaattt
      601 ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg aagtgaactt
      661 ctgaaaaccg aaaaactcaa gtattatctt caaaatacta gtactcaatt agatgctcga
      721 tttgattaca agaagactgc gaaagccact ggcaaccgca agctaggtat ccaccgatgg
      781 actacgtttt agaggcaact ggttatgaat acggagctaa cgctctcatc tgcataagaa
      841 tgcaacaccc agattatgta aagattttcg atacttctgt caaaaaaatc atcaagatag
      901 ataaatgatt tccgtctagt caaattggtt cagaatgtgg acacacagat ggtaagaagt
      961 cattcacaat tcgagaatgg acttgtccta tttgccgtac acatcatgat cgaaacgtca
     1021 acgttagtat caatattttg atcaagggct atgtatacaa gcattgcctt agaaaacatc
     1081 aagaacctta ggaacaacgg ggatagattg gttaataaga gatatcgctg ttagtaaaga
     1141 cataggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgataggttt
     1201 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataa
//
LOCUS       DANKHJ010000297         1235 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000297 DANKHJ010000000
VERSION     DANKHJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.310"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..344
                     /locus_tag="PW113_003521"
     CDS             <1..344
                     /locus_tag="PW113_003521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527737.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="PW113_003522"
     CDS             425..1045
                     /locus_tag="PW113_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527738.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
BASE COUNT          487 a          148 c          207 g          393 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtt
//
LOCUS       DANKHJ010000298         1228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000298 DANKHJ010000000
VERSION     DANKHJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.311"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            200..742
                     /locus_tag="PW113_003523"
     CDS             200..742
                     /locus_tag="PW113_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527739.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1228
                     /locus_tag="PW113_003524"
     CDS             898..>1228
                     /locus_tag="PW113_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2527740.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKAS"
BASE COUNT          510 a          136 c          236 g          346 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcat
//
LOCUS       DANKHJ010000299         1210 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000299 DANKHJ010000000
VERSION     DANKHJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.312"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..545
                     /locus_tag="PW113_003525"
     CDS             <1..545
                     /locus_tag="PW113_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527741.1"
                     /translation="TRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNDAVNV
                     YDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVIV
                     NGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYVRKDLGTIITN
                     SEFAANEWFTFHGNYWVGEE"
     gene            548..994
                     /locus_tag="PW113_003526"
     CDS             548..994
                     /locus_tag="PW113_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527742.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            996..1133
                     /locus_tag="PW113_003527"
     CDS             996..1133
                     /locus_tag="PW113_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2527743.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
BASE COUNT          429 a          179 c          271 g          331 t
ORIGIN      
        1 acacacgaac cccgaatatt agtcagttta aatactttgg tgaaggcttg aaagacagta
       61 acaatcccaa tgattacagt tgggatgtca cacctgaata tactgaaaaa ggcttgaatg
      121 atgctgttaa tgtgtatgat cctcagagag ttgaaggttt gaaaaacttt gccgatggta
      181 ttcaaatagc aggagataaa gtgatcagtg aaaatgattg cactgtttat acattaacta
      241 aagacaacag tcaatcgttt atcgatgggt atgcaacatt tattaaacac ggaaaagaag
      301 ttattgtaaa tggtacagta aagttcaaaa aagcttatgc ttttggtgta ccacttgatg
      361 atgaagtccc agatgaattt gtcgcaaaaa ttgttcatgg catgcttaca ggtccgtcag
      421 gaacaagtag tgtatcaaaa gcgatgtacg tgcgaaaaga cttaggaaca attattacga
      481 atagcgaatt tgctgcaaat gaatggttta cattccatgg taattattgg gtaggggagg
      541 aataaaaatg aaaaatattt ggaaatacgg acgaacaggt ggagagtatg ctggacaagt
      601 gcttgatgat atggttatga ctgttccatt taccgatgtt ccaccactcg aaggaattcg
      661 tgctgatggg gaaccactaa cgattgctga tcagatgttt gatcctaaac tgaaccaatg
      721 gattgtttta gtgaatgtac tagatcacaa cgatttaaac aatctcaaag cgatgtatga
      781 gtcgttagaa aatgagaacg gcgatttaaa acagctcaac gccaaactca tgctaagcga
      841 tgtagcgatt aaacaggaaa atactgcatt gaaagaaaaa gcggatggtt tagcacaaat
      901 caattcaaaa acgatgcttg cttcgttcca aaatagcaaa gacattgcag aaattaaaga
      961 gcaactaaat ccagcttcaa agggaggtga gtagtatgtt tagttttagc gatgtgaaaa
     1021 tgatgtatga ttggggctgt tttactgacg atcaagttcg actattcgtt ccactatgca
     1081 ttacagacga agaagcagaa aaaatcatta ataaagataa gagcgcatct taagtgatgc
     1141 gtttttattt aaggtaaagg agttgtcaca tgattaattt aggagaatgg ggaacaatcg
     1201 caggatcaat
//
LOCUS       DANKHJ010000300         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000300 DANKHJ010000000
VERSION     DANKHJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.313"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1076
                     /locus_tag="PW113_003528"
     CDS             <1..1076
                     /locus_tag="PW113_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2527744.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANKHJ010000301         1193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000301 DANKHJ010000000
VERSION     DANKHJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.314"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..446
                     /locus_tag="PW113_003529"
     CDS             <1..446
                     /locus_tag="PW113_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527745.1"
                     /translation="APPLPEITYGEFPFSLEYEIDEERILIEDTVIAEFIGSFRGNVT
                     TQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMNDGGSKPQLTVR
                     DRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            633..>1193
                     /locus_tag="PW113_003530"
     CDS             633..>1193
                     /locus_tag="PW113_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2527746.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT          392 a          143 c          242 g          416 t
ORIGIN      
        1 cagcaccacc attgccagag attacatatg gagaatttcc gtttagtctt gaatatgaaa
       61 tagatgagga aagaattttg attgaagata cggtgattgc agaatttata ggaagtttta
      121 gagggaatgt gacaactcaa cctagacgaa gttggagtat aaaactttta aataatagtg
      181 gagagttgaa agcatttcat tatgaattct taataataga aagtgaagat gtaacaatca
      241 ctttaagtag tggctctgct ggatatttta tgaatgatgg tggaagcaag ccacagctca
      301 cagtccgtga tcgtattgca ggttttatgc atttttctca ctatgactta gaagaagctc
      361 gtaaaatact tgcagaatat gggatcacat taatcaattg ggaatatgcg ccaccgataa
      421 gaaatttttt caatgattat tattagtctt ataaaaaaca gaatgtaaaa tttatgtgcg
      481 cgagtattta gttttccatt ttttgtttga tagatatata gcattgttga agcatgggat
      541 agtggtgcca attttatcaa tgaggttggt caggcagtta acataattgg tgatgctata
      601 aatccattta attggaattg gaggaattag gtgtggaaac agtgagatta tggatattca
      661 tgaaaaattt tcgttcggct ttgagatatg ttattctgga atcaaaatca ggttattatc
      721 tgattgacac agatcactat actttttggg gaggctacct ttttccgatt tttaattgga
      781 taatccccca taaggttatg aaaattaact tgcctgcaga agaaataaat agattaatac
      841 tcaacaaaga tttacaaagg aaaagagaga aagaacattc taatgttgct tggataggtc
      901 cagcatttgc agtagtgact tcttcaattg tcggtcctat gctagagagt atttatagtg
      961 atttttctat tggaatacgt tatttatttg ttactttaag tatagctgtt cttttgtgca
     1021 taaagggtta tgttagcaag aagaataaag atattttaac actaatcggt gtaaacaatt
     1081 tatctagtac taaaattata ctgtttcctg aatctataaa agaaggatca aggttgttga
     1141 taatttttat aatgcttata ctgttttctg ttttatttgg ggtgatgttt ata
//
LOCUS       DANKHJ010000302         1181 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000302 DANKHJ010000000
VERSION     DANKHJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.315"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            49..1128
                     /locus_tag="PW113_003531"
     CDS             49..1128
                     /locus_tag="PW113_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2527747.1"
                     /translation="MPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEE
                     STLRSWKTDKSDAHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMD
                     LHNCLQLTFPELEQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKIS
                     ANRAEQKADQIIHYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAE
                     GSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQD
                     RINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNK
                     LLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          388 a          274 c          215 g          304 t
ORIGIN      
        1 gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt
       61 ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa
      121 gatcatcact tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa
      181 agcaccttac gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat
      241 cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat
      301 ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac
      361 aactgccttc aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct
      421 tacgcattga cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg
      481 acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc
      541 gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa
      601 gacagtatcc actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa
      661 caaaaaaaag cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg
      721 ttgtaccaaa cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta
      781 ggagatatca cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc
      841 cgacggtatc attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc
      901 aaagcacgga aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct
      961 cctaatcata ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaagat
     1021 aaggttgcca cagttgcttg tatgaacaag cttctgaaat gtatgcacgc tatggttagg
     1081 gcacatacag agtacgacta tgcgtacgcg gtctcagtgg accattaact aaaccactta
     1141 cagtatacca cttctttgcc cagatcgaaa attttttacg a
//
LOCUS       DANKHJ010000303         1176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000303 DANKHJ010000000
VERSION     DANKHJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.316"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(445..687)
                     /locus_tag="PW113_003532"
     CDS             complement(445..687)
                     /locus_tag="PW113_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527748.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(674..1102)
                     /locus_tag="PW113_003533"
     CDS             complement(674..1102)
                     /locus_tag="PW113_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2527749.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT          375 a          234 c          186 g          381 t
ORIGIN      
        1 attcggaaca ggaatcaact tgagtacagt aaaaaaactt aaaagtggag agagagctga
       61 agaaaaatta aatcttgccg atgctattaa aatcacagaa tttggcatga aaaatatgcc
      121 aaccaaaatc gaaatatgga aatagtagtt tttctgatct atcagctgtt ttgccaaagc
      181 acataggaat ctcacctccc cccagtaatg gcgggccgat ttggttacgg aagtatcatt
      241 atcgtagtcg cgattctcat attaaaattt gtcgcttaaa agctaacctt gtcatggcgt
      301 aactagagaa tcaaaatcta catgtactga tagatttgct attcatttgt caaagagcgc
      361 taggctattt tcagcctatg gaaatccttt attttgaccc gataaaaaac ttccatagaa
      421 tgaaaaggaa agggaaaaaa tactttaacg agttgtatca aattcaagta tcttctcaat
      481 caattttgtt tccagttgtc tacgcatata ttcatctaca tacataacca ttcctttttc
      541 ttcttcataa actggtttca tggctaaccg tgaaatataa ttttgatatt ttgacagaac
      601 gatggacatg gcaattgaat caccagaagc tgcggctttg attgtcgtta gtggtatgaa
      661 tggtatatat cgtttatttt gcttcatatt tgacacccaa atactttcct agctcttcca
      721 aagccatttg tcgttggtac caaacactgc cgagactttt cccaataata tcgccaattt
      781 cacgatcatt aagaccgatg aagaaggaca taaaaatgat ttcttgtctt tgactaggta
      841 atgctgcaat cgcatttgcc aactcctcat tggaaataaa gattttcata ccaaggacaa
      901 taaaagtacc actactattc aaatttggtt ccaaataata gaactctgta cctaaccctt
      961 tttgttccaa tatttccatt gatatttgcc gttggcgaaa tctagcgtac ttcttttgaa
     1021 tatttaacgc ctcattgcag actactttct tacaaaaact gtcaaacgta tgttgaatgt
     1081 gggtttcctc tgagtcgccc ataatattct ccccctcaaa aaattcattc aactcgtttt
     1141 acttctatcc ctttccatta ttactacaat ctagga
//
LOCUS       DANKHJ010000304         1135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000304 DANKHJ010000000
VERSION     DANKHJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.317"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(51..581)
                     /locus_tag="PW113_003534"
     CDS             complement(51..581)
                     /locus_tag="PW113_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2527750.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(680..1030)
                     /locus_tag="PW113_003535"
     CDS             complement(680..1030)
                     /locus_tag="PW113_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2527751.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          410 a          205 c          181 g          339 t
ORIGIN      
        1 ttttttggat tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata
       61 gtcatttttc ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata
      121 aagccgaata tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat
      181 ccgccaatca acaacagaat actactataa acactccaac ttttagcttt tgtattcatg
      241 ctgaagaaca actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg
      301 atagaaacga ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga
      361 ccactgaaca gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa
      421 agattcaaca acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa
      481 acagcagaag ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat
      541 aaggcgtatt tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga
      601 cttattttaa cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag
      661 aagaatatct ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta
      721 gctcatcgat cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt
      781 ctactcgtac accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg
      841 cttccgcaaa aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt
      901 gggaataacc cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc
      961 tctccgcttt tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat
     1021 tttgcgccat aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa
     1081 gaaaaagaga agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaat
//
LOCUS       DANKHJ010000305         1123 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000305 DANKHJ010000000
VERSION     DANKHJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.318"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..436
                     /locus_tag="PW113_003536"
     CDS             <1..436
                     /locus_tag="PW113_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2527752.1"
                     /translation="ESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCL
                     KLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLR
                     DLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            512..730
                     /locus_tag="PW113_003537"
     CDS             512..730
                     /locus_tag="PW113_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527753.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            717..>1123
                     /locus_tag="PW113_003538"
     CDS             717..>1123
                     /locus_tag="PW113_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527754.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGY"
BASE COUNT          420 a          144 c          205 g          354 t
ORIGIN      
        1 agaatcacat aatgatgatc ttcttgaatc actttcaact aaaaataata aattagatga
       61 agaattgatc gaaacattta ttgaagaaaa tcttcttaaa caaatatggg gagaaagtat
      121 tgtgaattgc ttaaaactgg ctagcaatag tgattatcgt caattcgaca actggtacaa
      181 aaaattcaaa tatgcgatta gatctgctga aaaagaacaa aaaattcaac tgaaaattat
      241 ctatgaaatt tgtaataaca agtattttgt agatcatgtt agagaacagc tatcaatgac
      301 tttacgtgac ttgatacgaa gagcaaaaac agatcatcgt atcaaacaaa aagacgatta
      361 tatctttgct tctctaaaaa ataaagcgtt agaactaatt gaattgcaaa tcagcgaagg
      421 aatagataag caataagaat agaattgagt caaatgaaat ggaggggtat ctccacttca
      481 tttgagaact ttgaatatag gaggggttat tttgattaaa aatcgtttaa tagatcaagt
      541 ttggtgtcta gatcaaaata cagatagtct cgaattaatc gagactattt gcttcaacac
      601 tatagttctc gatttaagag ctgaaaatga taattgtgta actcatgtca taattaatca
      661 aaaaatggct cagaagctct ctgagagcct cagaaaatgg gctaagggag gtaacgatga
      721 aaataattga aaaaaagatt gccaataaaa aaataaattg gttggttgtt aattttcttg
      781 ttctgatagc tatgattatt tttacaagta atattgctca tgcgagtatt ttcaattggt
      841 ttgatgacac aaaaagtcaa acagagtttt tgaataattc tctttataga ccttacctaa
      901 aaaagtacaa tggagatttc cttagtcagt tcgggatttg gctcggatgg gctgtggtaa
      961 aaggcatgtt tactgtgaca gattcgattc aaaatatgat tccagatgtg ctggatttgt
     1021 tcaatttcat tgaaagtaca gggctaaata atgtttatca atcagccgta aatacaattg
     1081 tcattggatt aatgatcctt tcattgatgt ttgttggtta caa
//
LOCUS       DANKHJ010000306         1108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000306 DANKHJ010000000
VERSION     DANKHJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.319"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            164..715
                     /locus_tag="PW113_003539"
     CDS             164..715
                     /locus_tag="PW113_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDL2527755.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          416 a          158 c          212 g          322 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaa
//
LOCUS       DANKHJ010000307         1102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000307 DANKHJ010000000
VERSION     DANKHJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.320"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          373 a          178 c          232 g          319 t
ORIGIN      
        1 cgtgaagcta ttccattccc acgtttgttg aaccgaattt atccataaga ggtgatgaaa
       61 aagctgttct gcatcaagta attagaacag ccaagcaaaa acagctgttc atgtttttga
      121 attggcggga cttattaccg cagatgtaag cttttgaata ccgtttagtg gagaggttgt
      181 gaaaaagctg tttagtttcg agtattaaga aaaattttgt ggaaatggct ttccacattt
      241 ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta aggaagaggt
      301 tgtgaaatca gtgccttgac ctgagtatta gagaaacaaa acggaaaaat agcttctcat
      361 attttaccgt ttttttagct aatatcgagg gtcagctgat tgaacaccgt ttatatcaag
      421 aggttgtgcg cctgccgttc agctccgagt cacaaggagt aatttcaaaa aacagttttt
      481 catgtttgat gactattgtg acttgtgacc gaggagttgg ccggagagca acgtttattt
      541 aaaaaaaaga actgcgaact ctcgcagttc tttttttaaa atacgggata aaggaagttt
      601 ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg attcacaagg ttctataacg
      661 aaatcgcaat caatcaaaca tatacaaaga aggttattca aatggaaatc tgatttgatg
      721 atgcttctgc tgttgaaata aagcagcatc tagtacctga aaacaaacta atagtaacat
      781 tcgaagatgg agtaggtccg tattttcagc atgcaatgat ccatatgtaa gtttaattca
      841 tccttgaaat catcggacca gataaagtta tgaccaatct atcccaagca atctagaaga
      901 gatattagta aaagaacatt tactggaagt cttagatgag cctataaata tatgttctga
      961 tggcgtcatc agtggtctga ttgacggaag cttaggtgtg atcgatttct ctggaccaaa
     1021 caacgaagga atcaagggga atccggcacg ttaagaaaaa aagtataaaa aactagacac
     1081 ttgatgagcg ttcattcaat tg
//
LOCUS       DANKHJ010000308         1098 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000308 DANKHJ010000000
VERSION     DANKHJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.321"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(1..254)
                     /locus_tag="PW113_003540"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="PW113_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(286..447)
                     /locus_tag="PW113_003541"
     CDS             complement(286..447)
                     /locus_tag="PW113_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL2527756.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(771..>1098)
                     /locus_tag="PW113_003542"
     CDS             complement(771..>1098)
                     /locus_tag="PW113_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527757.1"
                     /translation="NREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKEGF
                     KTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMAQF
                     RSENVN"
BASE COUNT          311 a          237 c          145 g          405 t
ORIGIN      
        1 gtcccttaat tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt
       61 tcttcttcca aagataatcg aggactttgc tgtactcttg cacccaacga taaatcgtag
      121 tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga tagcttagat
      181 tgtaacgcag gtagtaacta acagcgacaa taatgacgtc attttttgaa ttgtttgcct
      241 ttaaaatgat tcattactct atcctctctg tcttttttct caattttaca ctaaaataga
      301 ttttttggaa aactttgcaa cagaaccact tttcgtaata tcaatgtctt tattcgtatg
      361 atttttcgtt ttacgatcta catgattttg aagtccattt aacgacccaa ctttatgctt
      421 ttgtgttcgt gccaccatat aggccataac tgtgtacttc ctttcgttaa aattattgcg
      481 agaatcacaa gctaaattcg gaaaaacctc catgtaaggt ataacttact agacttatac
      541 tgcgtaaagt ctgtaagctc tgccgagggc tatatcggct taacagccga tgagaaactc
      601 cgtttctctc aaacgaaaaa gcgaacgaaa aacgactgcc ttcgcattcc ttttttacgt
      661 ccttttttcg ttcaaaacca ttggcttttt gcgtcattgt tttcacaatt tttattccgc
      721 aaaaaaccaa aagtgaaaag tattgctttt cacttcaacc cctcctcaat ttagttcaca
      781 ttttctgaac gaaattgtgc cataaattga gtcaaaccct taacatcatt cgtacgccaa
      841 tatgcaccat tttcttgcca aactaatcca tctgcaaatt ttcttagcga tactaaaacc
      901 tcatcattta ctgaatgatt tcttttattt gattccaaaa aatctaatgc ttcagcaggt
      961 gtcttaaagc cttccttttt acaatataat ttcgctaaag aaatcatttc tttctgtcta
     1021 atctgttttc gtttcgctct aatcttcgct tcttttgctt tcaaatccgc catttctttt
     1081 gccaatattt ctctattg
//
LOCUS       DANKHJ010000309         1098 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000309 DANKHJ010000000
VERSION     DANKHJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.322"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..569)
                     /locus_tag="PW113_003543"
     CDS             complement(<1..569)
                     /locus_tag="PW113_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527758.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLI"
     gene            complement(682..939)
                     /locus_tag="PW113_003544"
     CDS             complement(682..939)
                     /locus_tag="PW113_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527759.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          320 a          246 c          147 g          385 t
ORIGIN      
        1 ttgattaagg taattccgta ttgtgtcaaa acttcttgcg cttctccgaa attaagtaca
       61 gttcttatgt atccactttc tcctaatata aagtctacct ctcttttaaa accttcctcg
      121 agatttctaa gggctatatg tggttttaat gacattgatt gtacattttc ccccatataa
      181 tattgcgcac ttcctcgact aaaatagatt cctagatcgt ccccaaaagg tagatcttct
      241 ctaccactag ctaatctact tctccatctt cgttctcttt tcatagaacc tgcgctgaac
      301 tcacacccgt taaactcagc tatgacagta tcttcaatta tgttaatctg tccatctagt
      361 tcgtattcaa tcctaaatgg aaattctcca taggtgattt ccggttcggg tattttaggt
      421 gagattaatg caaagaaagg aaatattaag cttggcaaca tcaaaaaaac tcctcctatg
      481 atcacaattc ccgatgtaat ccaaaatata atttgtacaa ttctcattga tccattgtat
      541 tccattttat tccccctatt actattcata tccgcctatc tatatcgatc ttccaatgca
      601 gagataattg cttccatacc ttgatttttt actgagttac ctatgcgaga tatcattctc
      661 cactaaggtg catcatattg tctatctaac atgattaatt ctccaatagg tgtatactcc
      721 tccaaaaaca acatttaatg catctccctc gacaacataa gctaccatgt ttgctgtata
      781 tgtgtttgag tctaattcat aagcagataa atttactgtc gctggattaa atataatcgc
      841 attcgtattt atagctactg aatttgctgc ttcagcacca ccttttgaat gtccgatcat
      901 tgtgatttca atagtaggat ttacgtggac agccctcacc gtttaccctg accattatag
      961 aaatcgctta cttttttgaa ctacgcaaca aaggatacaa tgctcctaac ttcactgtct
     1021 ccatttttgt ccactaagga atataaaaaa taggcattaa ttcatttaat tcggtcacaa
     1081 cacgttacct tttaaatc
//
LOCUS       DANKHJ010000310         1069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000310 DANKHJ010000000
VERSION     DANKHJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.323"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..777
                     /locus_tag="PW113_003545"
     CDS             <1..777
                     /locus_tag="PW113_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2527760.1"
                     /translation="SQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPL
                     STEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAE
                     TQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYA
                     NYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQ
                     TYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            771..>1069
                     /locus_tag="PW113_003546"
     CDS             771..>1069
                     /locus_tag="PW113_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527761.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRS"
BASE COUNT          414 a          174 c          155 g          326 t
ORIGIN      
        1 tcacaatttt tagcaaaaga acttcaaaat ctagtagata gtaatataac agatacaacc
       61 ttattttttc aagataatag catttattat tccgataatg gacaaattaa actactttgg
      121 aaatcacctt taagtacaga aaaaaatggt gtttcttccc ttacagaaaa aaatgatctt
      181 atatccgtaa atataaaaaa taaaattaaa aatttttcta aaaaacaaaa ttttcctgcg
      241 gttaaacgtg taaaaagtgt ctcctccgga gataacttta ttatgattaa ttggcgtata
      301 gcagaaaccc aaacaagttt tccgtggtgc gctgcttacg tagaagctgc tatcttatgt
      361 aacaaaacag atgtacgtgc tacttcagct agagccatta tgcattatac ctatcctaat
      421 ttaaacaaca gtcaaattga atcaaaaagt cttaatcaag atcaaataat aaaatatgca
      481 aattactgtg gtgtttatcc taaaaaaata gcacgaatac taaacctttc agaggtccaa
      541 acacaactaa gaaaaggaaa tgccatttat atgggaactg ctggttatgg aaattatagt
      601 aacagccgtc atgcacttgc attatttggg tgggtcaatc aaggtggtta tcaaacttac
      661 tatgtgtgga atccttggta taactaccca actgtaataa gtgcaaatgc aggaccagtt
      721 acaattcctg tacctggagg tggttttact tgggacaata ccattacaaa ttggtaaata
      781 aactcattgc tttagttatg ggtctagtgt tagttctttt cttaactagt tgttcaaaaa
      841 aacaagaaat acaaaagact gatctgattc cttttctagg aacttggcaa actaaagatc
      901 attcagaacg tctagtaatc actaatttaa atacttcaaa aaatatagaa caatatacca
      961 tcttagtaaa agagaacaat cataaaattc aaatcattga taaaaattta aatataaatg
     1021 aacaagtttt catttacaaa gcaacaaata atttactaac actgcgctc
//
LOCUS       DANKHJ010000311         1069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000311 DANKHJ010000000
VERSION     DANKHJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.324"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..777
                     /locus_tag="PW113_003547"
     CDS             <1..777
                     /locus_tag="PW113_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2527762.1"
                     /translation="SQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPL
                     STEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAE
                     TQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYA
                     NYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQ
                     TYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            771..>1069
                     /locus_tag="PW113_003548"
     CDS             771..>1069
                     /locus_tag="PW113_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527763.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRS"
BASE COUNT          413 a          174 c          156 g          326 t
ORIGIN      
        1 tcacaatttt tagcaaaaga acttcaaaat ctagtagata gtaatataac agatacaacc
       61 ttattttttc aagataatag catttattat tccgataatg gacaaattaa actactttgg
      121 aaatcacctt taagtacaga aaaaaatggt gtttcttcct ttacagaaaa aaatgatctt
      181 atatccgtaa atataaaaaa taaaattaaa aatttctcta aaaaacaaaa ttttcctgcg
      241 gttaaacgtg taaaaagtgt ctcctccgga gataacttta ttatgattaa ttggcgtata
      301 gcagaaaccc aaacaagttt tccgtggtgt gctgcttacg tagaagctgc tatcttatgt
      361 aacaaaacag atgtacgtgc tacttcagct agagccatta tgcattatac ctatcctaat
      421 ttaaacaaca gtcaaattga atcaaaaagt cttaatcaag atcaaataat aaaatatgca
      481 aattactgtg gtgtttatcc taaaaaagta gcacgaatac taaacctttc agaggtccaa
      541 acacaactaa gaaaaggaaa tgccatttat atgggaactg ctggttatgg aaattatagt
      601 aacagccgtc atgcacttgc attatttggg tgggtcaatc aaggtggtta tcaaacttac
      661 tatgtgtgga atccttggta taactaccca actgtaataa gtgcaaatgc aggaccagtt
      721 acaattcctg tacctggagg tggttttact tgggacaata ccattacaaa ttggtaaata
      781 aacccattgc tttagttatg ggtctagtgt tagttctttt cttaactagt tgttcaaaaa
      841 aacaagaaat acaaaagact gatctgattc cttttctagg aacttggcaa actaaagatc
      901 attcagaacg tctagtaatc actaatttaa atacttcaaa aaatatagaa caatatacca
      961 tcttagtaaa agagaacaat cataaaattc aaatcattga taaaaattta aatataaatg
     1021 aacaagtttt catttacaaa gcaacaaata atttactaac actgcgctc
//
LOCUS       DANKHJ010000312         1066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000312 DANKHJ010000000
VERSION     DANKHJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.325"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(39..995)
                     /locus_tag="PW113_003549"
     CDS             complement(39..995)
                     /locus_tag="PW113_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527764.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          382 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaact
//
LOCUS       DANKHJ010000313         1036 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000313 DANKHJ010000000
VERSION     DANKHJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.326"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..173
                     /locus_tag="PW113_003550"
     CDS             <1..173
                     /locus_tag="PW113_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2527765.1"
                     /translation="ELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITELKHA
                     KELREEWFTNDD"
     gene            163..429
                     /locus_tag="PW113_003551"
     CDS             163..429
                     /locus_tag="PW113_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527766.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            588..866
                     /locus_tag="PW113_003552"
     CDS             588..866
                     /locus_tag="PW113_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527767.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(863..>1036)
                     /locus_tag="PW113_003553"
     CDS             complement(863..>1036)
                     /locus_tag="PW113_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2527768.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT          391 a          148 c          205 g          292 t
ORIGIN      
        1 acgaattaag aagtaaggta ggatcaaaca tccaaagagc catctatttc catgtggaca
       61 atggaaggta tgtcattact cacggattta ctaaaaaaac ggagaaaaca ccaataactg
      121 aactcaagca tgcaaaagaa cttagagagg agtggttcac aaatgatgat taaaaaattg
      181 attgaagaaa agaaaaaaga taaagatttt gcagaagcat atgaccgtga aggaagaaaa
      241 ctcgaaactg cggttgctct ttatcatgct cgggaggaag cagggctaac tcaagcggag
      301 cttgcacaaa aagcacatac aactcaatct actattgctc gaattgagag aggggataac
      361 gtatctttcg ataaacttga tgatatcgcc agggcattag gtaaaaagct ggtagtagaa
      421 tttgcttaaa agcgagttga aaatatatct ataataggct cctaactaat caattagttt
      481 agtgggcctt tttttactag atagataaag gttgtactat agtgtaaaag acaataaatt
      541 ttatactaaa attactttaa agagaactag agagaaaggt acaataaatg aaaaccataa
      601 atcttataaa agtatttata gcatcaccag gtgatatagc agcaaaaagg aatgaagtag
      661 aagaaaccat atatagatgg aatatagaaa atacagataa tacaaaagtg attttgatgc
      721 ctatacgatg ggaaactaat gcgattccta cttatgtaaa taatagctct gggcaaagtg
      781 ttattaataa acaaattgta gaaaataggg ttctgctgca aagttttaaa aataaaatca
      841 gtttttgtat tatttcttca ttttaagctg gaattcccaa taatatcctg atttcagtgc
      901 agactgaaaa gccgaagaga gtgccttctt ttcggctttt cttgtagatt cctcgaatgg
      961 tttccatccc tttaattgtg gtggaagctg tacgtagact ttgatacaat ttatgtcgac
     1021 gttttatcga tcgatg
//
LOCUS       DANKHJ010000314         1022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000314 DANKHJ010000000
VERSION     DANKHJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.327"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1013
                     /gene="xerS"
                     /locus_tag="PW113_003554"
     CDS             <1..1013
                     /gene="xerS"
                     /locus_tag="PW113_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2527769.1"
                     /translation="QDREDKDQSPRTTLEYLKNYKLFYEWLLSESIVPDTSITSIRQL
                     TAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLSTSTINRNITALKVLFKYLSK
                     SSNNPLGKPYLENNPMDQVATITDKTTLAARANAIEKKLFLDEDTQNYLDYIANEYKK
                     TLSKRALIYYQRDVERDLAINALILGSGLRLSEVVNINLEDLSLEKNNVVVTRKGNKR
                     DAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGIAIERMVAKYS
                     KGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHTSTNTTTLYTHIDNAATIDALN
                     AL"
BASE COUNT          403 a          173 c          165 g          281 t
ORIGIN      
        1 ttcaagatcg tgaggataaa gatcaatcac ctcgaactac tttagaatat ctaaaaaatt
       61 ataagctctt ttatgaatgg cttctgagtg agtctatcgt tcccgatact tcaatcacct
      121 cgataagaca gttaactgcc tatgatctaa acttgtacaa atctcatttg aaacgtcgca
      181 caaaggaaaa tgtaaaaaaa gagacaacaa aaactaagct caaagataat aataacttag
      241 gtctatctac ttctactatc aatagaaata tcacagcatt aaaggttcta tttaaatatc
      301 tttcgaaaag ttccaataat cctttaggaa aaccatactt agaaaataat cctatggatc
      361 aagtagcaac tatcacagat aaaactacgt tagctgcccg tgcaaacgca atcgagaaaa
      421 aattatttct agacgaagat acacaaaatt atctagatta tattgccaat gaatataaaa
      481 aaactttgag caaacgagct ttgatctatt atcaacgaga tgtagaaaga gacctggcga
      541 tcaatgcatt gattttagga tctggattaa gactctccga ggttgtaaat attaatttag
      601 aggacctatc tttagaaaag aataatgtgg tagtaactag aaaaggaaat aagagagacg
      661 cagtaaatat agcagcattt gcaatggaat atttagctaa ttatctagct attcggaaag
      721 aacgatacaa agtaacagaa aacgaaaaag ctttatttct agcaatttat caaggagaag
      781 ccaagcgtat atccggtatt gcaatcgaac ggatggtagc aaaatattca aaaggattca
      841 gagtccaagt cagtccacat aaattacgtc atactttggc tactagactc tatcaacaga
      901 caaattcatt ggtattaact gcccaacaat tgggacatac aagtacaaat acaacaactt
      961 tatatactca tatagacaat gcagctacaa ttgatgcttt gaacgctttg taaatgtagt
     1021 aa
//
LOCUS       DANKHJ010000315         1018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000315 DANKHJ010000000
VERSION     DANKHJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.328"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            115..>1018
                     /gene="bsh"
                     /locus_tag="PW113_003555"
     CDS             115..>1018
                     /gene="bsh"
                     /locus_tag="PW113_003555"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2527770.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNK"
BASE COUNT          358 a          147 c          191 g          322 t
ORIGIN      
        1 tactattttt tataaatgct agtaaatcaa tcttctttgg cctttttttt gataaaaaaa
       61 aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggaa aacgatgtgt
      121 accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg
      181 tcttataatg aagtagtcac tattactcca agaaattatc gtttcgattt tagaaaggta
      241 aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca
      301 ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc
      361 aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt
      421 ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc
      481 aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta
      541 gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat
      601 aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac
      661 aattatcgct cgctatcaaa tggaacacca gaaaatcatt tttcaaacca gatcagcttg
      721 aatgtttata gtcgtgggat gggaggattg ggcttacctg gagatctctc ttctgtttct
      781 cgatttgtaa aagccacatt tactaaaatg aatgctgcat caggtgattc agaatctgaa
      841 agtatcagtc aattttttca tatactaggc tcagtcgagc aacaaaaagg agtatgcgat
      901 actggagaag gaaaatacga atatacgatt tattcttctt gctgtaacgt tgataaaggg
      961 atttattatt atcgtacgta tgaagacagt caaattactg ctgtaaatat gaataaag
//
LOCUS       DANKHJ010000316         1014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000316 DANKHJ010000000
VERSION     DANKHJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.329"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>1014
                     /locus_tag="PW113_003556"
     rRNA            <1..>1014
                     /locus_tag="PW113_003556"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          272 a          229 c          310 g          203 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgt
//
LOCUS       DANKHJ010000317         1008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000317 DANKHJ010000000
VERSION     DANKHJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.330"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..491)
                     /locus_tag="PW113_003557"
     CDS             complement(<1..491)
                     /locus_tag="PW113_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2527771.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEV"
     gene            689..>1008
                     /locus_tag="PW113_003558"
     CDS             689..>1008
                     /locus_tag="PW113_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDL2527772.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWK"
BASE COUNT          307 a          210 c          180 g          311 t
ORIGIN      
        1 acttctcctt caatagaagc aagatgggca acaggtgcta aatccatcac ttcttgtaca
       61 ctgctttcag ctaacatagc aaatcctgtt tgtcttgcag ccatcacgtc tccgtgatct
      121 ccgaaaatat tcaacgcatt tgtcgtcact gcccgactag caacatggaa aacacttggc
      181 agtagttccc cagcaatctt gtacatattc ggtatcatca aaagcagccc ttgagaagcg
      241 gtatatgtag ttgtcagtgc accagttttc aatgatccat ggaccacacc agctgctcct
      301 gcttcggatt gcatttctac tacttttact ggttgaccga atacattttc ttttcctgct
      361 gctgaccatt gatctactaa ttcagccatg gtcgaactgg gtgttattgg atatattgca
      421 gcaatctcgg taaatgcata agaaatatat gcggctgccg tattgccatc cattgttttc
      481 atttttcgca tgattcatca tccttgttca tattctctct tattatctgt tccattataa
      541 cgtaaaacat tccatttttt cttttattta taaaaatatt tcacatatgt ctatttgaat
      601 tggtcatcta atttttagac aaacagtcta aatcgttgcc tttttttgtc agacatgtat
      661 aaactataag cgaaagaggt gttttaacat ggatatatca gtgattgatg caacgaaaac
      721 aaatacacaa aaaggaatcc tttatggttc atcgaatgct cctaaaaaaa tggtcgaatt
      781 catcaaccta tcttgtccgt attgccgaca atggtttgaa gaatcttatg agctattaga
      841 agaagcagtc caaagcgggc aattgcagcg agtgatcaaa ttgttcgata aagaaaaaga
      901 aagcttgcag cgaggaaacg tgatgcaccg gtatctcaca atcagcgatg gtcaaaaagc
      961 gatcaaagag atcaaacaaa tattcgatac tcaagacgag tggaaaca
//
LOCUS       DANKHJ010000318         1001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000318 DANKHJ010000000
VERSION     DANKHJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.331"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            370..1001
                     /locus_tag="PW113_003559"
                     /pseudo
     CDS             370..1001
                     /locus_tag="PW113_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          342 a          180 c          185 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctag tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DANKHJ010000319          993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000319 DANKHJ010000000
VERSION     DANKHJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.332"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..526
                     /locus_tag="PW113_003560"
     CDS             <1..526
                     /locus_tag="PW113_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2527773.1"
                     /translation="LKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKN
                     DYNFGGEQSGHMIFLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNI
                     RVSDKNGAMDVPAIKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYV
                     DKIAAVVKEEIGLD"
     gene            complement(598..>993)
                     /locus_tag="PW113_003561"
     CDS             complement(598..>993)
                     /locus_tag="PW113_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2527774.1"
                     /translation="ADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIEEGTPVI
                     GIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTTLTTVVP
                     TQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT          304 a          184 c          226 g          279 t
ORIGIN      
        1 attgaaaaaa gatacgatcg ttacgactgt catgagtaac ttaggattcc acaaagcggt
       61 ggaaggaatc ggtttgaaag atgtcgttac acaagttggt gatcgttacg ttgtagaaga
      121 aatgcgcaaa aacgattata actttggcgg cgagcagtct gggcacatga ttttcttaga
      181 ttttaacaca actggagatg ggatgctttc aggaattcag ttactgaata tcatgaaaca
      241 gacaggtaag aaattatcag agcttgcagc agaggtaaca atctatccgc aaaaattagt
      301 gaatatccga gtaagtgata aaaacggtgc gatggatgtt ccggcaatca aaaaagtgat
      361 tgaagaggca gaagctgaaa tgggatcaga aggaagaatc ctagttcgtc cttccggtac
      421 agaaccatta cttcgagtga tggcagaagc gcctactgat gagaaagtta attactatgt
      481 cgataaaatc gcggcagtag tcaaagaaga aatcggactg gattaagaga tattttcata
      541 aaaaataaaa gaacaaggac cagtgtcaga tgtctgacac tggtccttgt tcttttatta
      601 ttctactgtc actgatttcg ctaaattacg tggtttgtca acgtcgaatc cccgtaatag
      661 cgttgcgtaa tatgcaagta attgagtcgg tacgactgtg gttagagtag ttagataagg
      721 gtggacagtt ggtagcacca gctggtcact ttccttttct aatccctctg agacaataac
      781 tagtgtttta gcgccacggc tttcgacttc tttcaggttt cctcgagtat gcgcaccagt
      841 cacttcttca ctgatgattc caatcactgg tgttccttct tcgatcaaag caatcgttcc
      901 atgtttcaat tcgccagcgg caaaaccttc tgcctggata taactgattt ctttcaattt
      961 caaagcagct tcaagagaaa cgttgtagtc agc
//
LOCUS       DANKHJ010000320          989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000320 DANKHJ010000000
VERSION     DANKHJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.333"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..197
                     /locus_tag="PW113_003562"
     CDS             <1..197
                     /locus_tag="PW113_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2527775.1"
                     /translation="NNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGIPA"
     gene            280..897
                     /locus_tag="PW113_003563"
     CDS             280..897
                     /locus_tag="PW113_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527776.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
BASE COUNT          339 a          125 c          188 g          337 t
ORIGIN      
        1 tgaacaattt aatagaacaa gaccatcgac ctattaagcg acggaataaa ttttatcgaa
       61 gtctccggac tgcctcatcc acgattaagg ggatggagac cattcgagga atatataaaa
      121 agaaccgaag aaatggaacg ctcttcggct tttcggtgtc tactgagatc aaggtattaa
      181 tgggaatacc agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacagaac cattatttta aagtgggagg aaaattgttt tgtggatatt taaacaagaa
      301 atctttgcta aattatttag tttagatgga ttaggttggg gaatcttatt ttttattttt
      361 ttatttttag gatctatgtg gatctggttt gttgataaaa aaaattctta tgagaaagta
      421 atagtgaaaa aaatgaatcg aaatgaattc gatgattttt caaaggaaca tagagttgaa
      481 ttgtacgata gtcatgaaat taacagtact tctccatctt attattgggt atttggttgc
      541 gaagaaaaac cattggagat taaattgttg aaaatgcttg aaaatcctcc agtcattgaa
      601 gatttttctg caaaaaacgt tgaaaaacag atgtttgaac aggtacaaat aagaggtcaa
      661 aacaaaattt cattagaacc agatgaatat gcagcctttt ttttagaaac aaattcaggt
      721 acttcaatgc ctgttttcaa agttaaatta aaaatgaatg gtcgtttatg cacttatgat
      781 cttgttgcaa atatgcgcta tggttctact ggtgatagac gtactatttt aaaatttgag
      841 caaaccttta gaagtttttt gaaaaataga gtatcccaaa tttttaattt ggattgaaaa
      901 aattaaaaat ttggccaaaa atactacagt tttttagagc aatcatctta tgatgattgc
      961 tctttttttg ttgtttttgt gtcattcgt
//
LOCUS       DANKHJ010000321          986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000321 DANKHJ010000000
VERSION     DANKHJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.334"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>986
                     /locus_tag="PW113_003564"
     CDS             <1..>986
                     /locus_tag="PW113_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL2527777.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DANKHJ010000322          979 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000322 DANKHJ010000000
VERSION     DANKHJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.335"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>979)
                     /locus_tag="PW113_003565"
     CDS             complement(<1..>979)
                     /locus_tag="PW113_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2527778.1"
                     /translation="RQQLFKHFTEMDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGIL
                     TFADSVITGGTTIIAMILFIDWRLTLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFS
                     SINDKTQESITGIKVIKTFGQEKEDLADFEEKIEDAIIKNKRVNFLDSLFDPFITLII
                     GLSYVATIILGGKYVLEGTISIGQMVSFISYIGMLVWPMFAIGRLFNVLERGNASYDR
                     VNELLHEKTHIVEQLGAVQTPAKGELNVAISSFNYPKEESPALEQIKFAIQEGETLGI
                     VGKTGAGKTTIIKLLMREYDDYQGKIQFGGCSIKEYSLNALLGSIGYVP"
BASE COUNT          292 a          213 c          169 g          305 t
ORIGIN      
        1 tgaggcacat agccgattga tcctaataaa gcattcaatg aatattcttt gatcgaacat
       61 ccgccaaatt gaatttttcc ttgataatca tcgtattcac gcatcagtaa tttgatgatt
      121 gtcgtttttc ccgcaccagt tttcccaaca atacctaatg tttctccttc ttggatcgca
      181 aatttgattt gttctaatgc tggtgattct tctttcggat agttaaaaga ggaaatagct
      241 acattcagct cgccttttgc cggagtctgc actgcaccca gttgttccac gatatgcgtc
      301 ttttcatgca aaagttcatt gacgcgatca taagaagcat ttcctctttc caatacgttg
      361 aacaatcgac caatcgcaaa catcggccaa acaagcatac cgatatagct aataaatgag
      421 accatctgcc cgattgagat cgtgccttcc aagacatact ttccacctaa aataattgtt
      481 gcaacataag acagaccgat aatcaaggta atgaatggat caaataagga gtctaaaaaa
      541 ttgactcgtt tgtttttaat aattgcatct tcaatctttt cttcaaaatc cgcgagatct
      601 tctttttctt gtccaaatgt tttaatgact ttgatcccag taatactttc ttgtgtctta
      661 tcattgatac tagaaaaagc ttcttgcgaa tcacgaaaag catcgtggag ttttgacccc
      721 agcactcgtg aagtaacggc tagtaaaggc agaggaatca gcgcaatcag tgttaagcgc
      781 caatcaataa acaaaatcat cgcaatgatc gttgttccgc ccgtgatcac tgaatctgca
      841 aaggtcaaga taccagctcc tgctacattt tggattgcat tcaaatcatt cgttgcatga
      901 gccatcaaat ctccagtccg atatttttgg taaaagagat tgtccatttc tgtaaaatgt
      961 ttgaatagct gctgccgaa
//
LOCUS       DANKHJ010000323          977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000323 DANKHJ010000000
VERSION     DANKHJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.336"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            172..>977
                     /locus_tag="PW113_003566"
     CDS             172..>977
                     /locus_tag="PW113_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2527779.1"
                     /translation="MFEVFEGINSTYSSVGSEALYQRLRSFDFGEDYQLEKLIAFYQE
                     NPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRVGLFIFLGLLPLVGVGLLLLG
                     QVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVSTISAANQLAKMTTPLQKELK
                     QQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLPFISYHFVLTKLEKHSTEAIK
                     LWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKDIYHPLLKEAVVNP"
BASE COUNT          317 a          159 c          213 g          288 t
ORIGIN      
        1 gacatttttt cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct
       61 tatcaaccga gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa
      121 ttccgttcat gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa
      181 gtatttgaag ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt
      241 ttacgtagct ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag
      301 gaaaatcctc aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat
      361 cataattttg caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt
      421 ttatttattt ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa
      481 gtactaggaa tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg
      541 aagaaacaaa cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca
      601 gccgccaatc agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact
      661 aaaccattaa gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa
      721 gcagaattga tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat
      781 ttcgttctca ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg
      841 ggagaattgg aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc
      901 cgaccagagt ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa
      961 gaagcggtag ttaatcc
//
LOCUS       DANKHJ010000324          971 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000324 DANKHJ010000000
VERSION     DANKHJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.337"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..84)
                     /locus_tag="PW113_003567"
     CDS             complement(<1..84)
                     /locus_tag="PW113_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527780.1"
                     /translation="MPITILTGYLGSGKTILLNHILYGDHGK"
     gene            210..428
                     /locus_tag="PW113_003568"
     CDS             210..428
                     /locus_tag="PW113_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527781.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            449..>971
                     /locus_tag="PW113_003569"
     CDS             449..>971
                     /locus_tag="PW113_003569"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2527782.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTE"
BASE COUNT          329 a          183 c          181 g          278 t
ORIGIN      
        1 ctttccgtga tcaccgtaca gaatatgatt tagaagtatc gtttttcctg aaccaagata
       61 gccagttaag attgtaatag gtattttttc atagggaacc tctttctata ccttgaattt
      121 acactttaag tgcaacacat aaaaatgagt cataatcgat atccaactag tataagtttg
      181 tcgagaacac tcctagaagg aaaataatta tgagccagta ttgtcatctt actatactca
      241 aacgagaaag aatctttctg cttcacgaag aaggattttc tattagtata attggcaacg
      301 acattcagca tagcccgttt actattttac atgaactgag aagaaacaag aaaaaaacta
      361 atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga aaaaaatgcg gacgttatca
      421 cattctaaat gatccgtgag tttggtcaat tgttcgtcat ttatttgtcg attttcagtg
      481 gtcgccagag aaaatctcga atcgtttgaa gcatgaacat atggactttt caattagttt
      541 tatcacgatc taccgtgcaa tttgcaatga tcttttcgat acagaacccc tctcacacgg
      601 caataaaggc ctgatccgtt cattgcacca tcgaggaaaa acaagacata caaaaaatca
      661 tgtcgagtct cgtgggaaga tttgccttac tcattcgatc catggccatc caaaatccgc
      721 agatgataga agtgattttg gtcattggaa agccgatatt gtagctggca aaactagtgg
      781 tacttgttta gtgactttga cagacagaaa atcatgcttc cttttagcag gaaagaaggt
      841 ccccagaaag aaagccgctt atgtgagcga gaaaatgatt gaacttttaa gcagtattcc
      901 aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa gaattctcca atcatttcgc
      961 agttacagaa g
//
LOCUS       DANKHJ010000325          965 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000325 DANKHJ010000000
VERSION     DANKHJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.338"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(202..>965)
                     /locus_tag="PW113_003570"
     CDS             complement(202..>965)
                     /locus_tag="PW113_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2527783.1"
                     /translation="WGGELLIEILDFVDFDALQPKWIVGYSDISTLLLAVTLKTGIAT
                     AHGTNLIDLRGEYLDETTAMWESVLKTREGESILQQSSEKYQKVWPHEHSSSSVFHLT
                     EPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGTPYGDVQSFKRKYNDGKPILWFLENC
                     ELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAAEDLLDYKMKDVYEDLSKELKLPILY
                     DIDCGHVPPQLTLINGAYAEVAVEDRKATVLQTFI"
BASE COUNT          286 a          216 c          148 g          315 t
ORIGIN      
        1 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
       61 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
      121 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
      181 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
      241 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
      301 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
      361 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
      421 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
      481 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
      541 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
      601 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
      661 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
      721 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
      781 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
      841 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
      901 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
      961 ccaag
//
LOCUS       DANKHJ010000326          952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000326 DANKHJ010000000
VERSION     DANKHJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.339"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            134..307
                     /locus_tag="PW113_003571"
     CDS             134..307
                     /locus_tag="PW113_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527784.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          326 a          149 c          117 g          360 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aa
//
LOCUS       DANKHJ010000327          948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000327 DANKHJ010000000
VERSION     DANKHJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.340"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..272)
                     /locus_tag="PW113_003572"
     CDS             complement(<1..272)
                     /locus_tag="PW113_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2527785.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNP"
BASE COUNT          269 a          200 c          150 g          329 t
ORIGIN      
        1 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
       61 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
      121 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
      181 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
      241 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
      301 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
      361 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
      421 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
      481 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
      541 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
      601 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
      661 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
      721 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
      781 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
      841 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
      901 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataat
//
LOCUS       DANKHJ010000328          943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000328 DANKHJ010000000
VERSION     DANKHJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.341"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>943
                     /locus_tag="PW113_003573"
     rRNA            <1..>943
                     /locus_tag="PW113_003573"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DANKHJ010000329          919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000329 DANKHJ010000000
VERSION     DANKHJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.342"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(257..>919)
                     /locus_tag="PW113_003574"
     CDS             complement(257..>919)
                     /locus_tag="PW113_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2527786.1"
                     /translation="SINEGDVVCIIGPSGSGKSTFLRCLNRLEEPSDGEIIIDGAHLM
                     DKSTDINLVRQHIGMVFQHFNLFPHLSILENIILAPMDLKKESREEAEKKAIKLLQTV
                     GLEDKKDMYPEMLSGGQKQRVAIARALAMNPDIMLFDEPTSALDPEMVGDVLNVMKKL
                     AEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFLEDGTPQQIFENPQNERTKDFLNKVL
                     NI"
BASE COUNT          255 a          184 c          188 g          292 t
ORIGIN      
        1 ttttgtagtt gtcatgtttg taccaacaaa aaagccagct ttagaataag ataatcatca
       61 tagtttctga aggtggtatc gactatgtac ttgatatcgt caatgaaccg atggtacttt
      121 cttatgcgat ggaacaggta gagcgattgc ttgtttgtgc aggagaacaa gcgatgcggc
      181 tggtacagtt aagtgaaaaa acaagaaact aaaaatgccc ggtcgtcatg aagcgaccgg
      241 gcatttttgg tttttattaa atatttaata ctttattcaa gaaatctttt gtccgttcat
      301 tttgcggatt ttcaaagatt tgttgaggcg ttccgtcttc taaaaagttt ccgtcgtcaa
      361 tgaacatgac acgattggcc acttctttag cgaagcccat ttcgtgcgta acgataacca
      421 tcgtcatacc ttgttctgct aactttttca tgacgttcaa gacatcaccg accatttcgg
      481 ggtcgagtgc agatgtaggc tcatcaaaaa gcatgatgtc agggttcatc gctagagcac
      541 gagcaatagc cacacgctgc ttttgtccac cggaaagcat ttcaggatac atatcttttt
      601 tatcttctag accaactgtc tgcagcaatt tgattgcttt tttctctgct tcttctctag
      661 attctttctt caggtccatc ggtgctaaaa tgatgttttc caaaatagaa agatgaggga
      721 ataggttaaa gtgctggaat accattccga tatgttgtct caccaagttg atatctgtgc
      781 ttttatccat caagtgggcg ccgtcaataa taatttcacc gtcagaaggt tcttctaaac
      841 gattcaagca acgcaaaaat gtactttttc ctgaacctga agggccaatg atacagacga
      901 catctccttc gttgatgga
//
LOCUS       DANKHJ010000330          918 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000330 DANKHJ010000000
VERSION     DANKHJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.343"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..435
                     /locus_tag="PW113_003575"
     CDS             <1..435
                     /locus_tag="PW113_003575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2527787.1"
                     /translation="MKKVAGTLRLDLASYRELEAFTQFGSDLDAATQAKLNRGRRTVE
                     ILKQKLHAPLAVEKQVVILYALTHGFLDSIPVDSILDFEHELFEYLDTNHSDIFETIR
                     TTKDLPEEEALNSAIQEYKDMFLATKGSNSSTEDKLKSIQNA"
     gene            449..>918
                     /locus_tag="PW113_003576"
     CDS             449..>918
                     /locus_tag="PW113_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2527788.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQP"
BASE COUNT          316 a          173 c          189 g          240 t
ORIGIN      
        1 atgaaaaaag ttgctgggac tttacgccta gacttagcaa gttaccgtga attagaagca
       61 tttacacaat tcggttctga cttggatgca gcaacacaag caaaactaaa tcgtggacgc
      121 cgtacagtag aaatcttgaa acaaaaattg catgcaccgc tagctgttga aaaacaagta
      181 gtgattttgt atgctttgac acatggattc ttagatagta tccctgtaga cagtatttta
      241 gacttcgaac atgaattatt cgaatatctg gataccaatc attcagatat ctttgaaaca
      301 atccgtacaa caaaagatct tccagaagaa gaagcgttga acagtgcgat tcaggaatat
      361 aaagacatgt tcttagctac aaaaggaagt aattcctcta cagaagataa attgaaatcc
      421 attcaaaatg catagtgagg tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg
      481 tatcgcttca acgaaaaaaa cgagccaaat cacgcgggca atgcagatgg tgtctgcttc
      541 taagttgaca aaatctgaag cttcttcaca gaagtttcag gtttatgcaa caaaagtaag
      601 agaaatcgtt acgcatttga ctgctactca gttaaatgat attgcctcag aaaatcctag
      661 aggggatatc aattacaaca gcatgctgct cagccgtcct gtgaagaaaa ctggttatat
      721 cgtcatcact gcagatggcg gtttagtcgg tggatataac agctcgatat tgaaacaaac
      781 gatgtctatt ttagaagaag accaccaatc acccgatgat tacgtgatga tcgcaattgg
      841 cggaactggt gcagacttct ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa
      901 tctatctgat cagccaag
//
LOCUS       DANKHJ010000331          896 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000331 DANKHJ010000000
VERSION     DANKHJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.344"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            138..>896
                     /locus_tag="PW113_003577"
     CDS             138..>896
                     /locus_tag="PW113_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2527789.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNN"
BASE COUNT          349 a          140 c          175 g          232 t
ORIGIN      
        1 agtcctcaag gaagagaccg ctgccatacg ggctatcgac attattggga aagaatcgat
       61 taaaagatta ccatttagat caattaattg tctgatagaa taagtacgtg agggagaaat
      121 tgaaaggggg aaatataatg aaaaaatggc tgttattact aataggaatg ggaattttat
      181 tcactggttg tacatctgag aatacgagga ctaaagcgac tatagaaact gatacaacaa
      241 ttgagactaa aaaaagtaaa acgcaatcaa ctgaacaaac tacaacagaa atgaagcaat
      301 ctgtggaaac aagtgcaagt tcaatcactg aatcttcttc tgaagaggtt aagaatactt
      361 tgtgggatac gaataaagct tccaaattag aaacttttgt cacacaatgg ggaaagacat
      421 taggtcaaga atataaaagc tacaatcttc agaataatgt aagtctgtat ggcactcctt
      481 taccacaagc cgtgattaat ggcgattgga aaatggcaat caatgaagca ccagttaccg
      541 tacaatggtc agaagatgga actggtcatg cagatttcaa tttagtagca gtttattctg
      601 atgtagaaac tggagaatat ttagggcagc atgtttactt tttcggtttt caaaatggac
      661 aacctaaagt ttatttaacg caacaaaatc aaggtaatga aaataattat ctgtacttta
      721 acgaaactca aaatcaacag ttaaaacaag gtttcagtga tattgtgaat ggacaaactc
      781 ctcaaacacc agtagtagaa gaatctactc aacaagcaac aactacaacg acacaatcta
      841 caagtagttc aggaatttca agttgggaag ctgctaaaga atctgtgcta aataat
//
LOCUS       DANKHJ010000332          883 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000332 DANKHJ010000000
VERSION     DANKHJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.345"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>883
                     /gene="prfB"
                     /locus_tag="PW113_003578"
     CDS             <1..>883
                     /gene="prfB"
                     /locus_tag="PW113_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_122645302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2527790.1"
                     /translation="SGGLFDLDQLEEDIAEAEAKMAEPGFWDDSEAAQKVISENNANK
                     ETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRINALDEKMKTYELSMLLDGP
                     YDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGYQVETLDYQAGDEAGIKSVT
                     LLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCSVDVMPELDETIDIEINPDD
                     LKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQRSQLKNREQAMGMLKAKLY
                     QLEVEKKEQEAAALRGEQ"
BASE COUNT          303 a          164 c          219 g          197 t
ORIGIN      
        1 ttcaggaggt ctctttgact tagatcagct ggaagaagac atcgcggaag cagaagcaaa
       61 aatggctgaa cctggttttt gggatgacag cgaagccgca caaaaagtga tcagcgaaaa
      121 taacgctaat aaagaaacct atgaccaatt ccattctctt gccgaagaac tagaagaaat
      181 cgaattgatg gtggagatgc tgcaagaaga acctgatgca gcaatgcaag aagaagcaga
      241 aacacgtatc aatgcattag atgaaaaaat gaagacgtat gaattatcta tgctgttaga
      301 cggaccttat gatcgaaaca atgcgatcgt cgaattgcat ccaggggctg gcggaacgga
      361 gtcccaagac tggggcagca tgcttttacg tatgtataca cgttgggcag agcagcatgg
      421 ctatcaagta gaaacactag attatcaagc aggtgatgaa gcagggatca agagtgtgac
      481 cctgctgatt aaaggacata acgcttttgg ttatctaaaa tcagaaaaag gtgttcatcg
      541 ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt catacttctt tttgttcggt
      601 agatgtgatg ccagagcttg atgaaacgat cgatatagaa atcaatccag atgatttgaa
      661 aatcgatacg taccgagcaa gtggtgcagg tggtcagcac atcaataaaa cagaatctgc
      721 tgtacggatc actcacttgc ctacaggaac agtagtagct agtcaggcac aacgctcaca
      781 attgaaaaac cgtgagcaag cgatggggat gttaaaagct aagctttatc aattagaagt
      841 ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa caa
//
LOCUS       DANKHJ010000333          845 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000333 DANKHJ010000000
VERSION     DANKHJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.346"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>845)
                     /gene="infB"
                     /locus_tag="PW113_003579"
     CDS             complement(<1..>845)
                     /gene="infB"
                     /locus_tag="PW113_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2527791.1"
                     /translation="VTIMGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDG
                     KPITFLDTPGHAAFTSMRARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIV
                     AVNKIDKPGANPQHVMQELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAE
                     VEDLKADPTQRAIGTVIEARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTN
                     DLGRRDKEAGPATPVEITGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAAT
                     SRVTL"
BASE COUNT          191 a          207 c          183 g          264 t
ORIGIN      
        1 tccaaagtca cacggcttgt ggcagcacgg ttttccaata atgcacgttt tgcgcgttct
       61 tcacccgctg cacgtgctgt cttttcgtct tcgaagacta cgaaacggtc acctgcttga
      121 ggtacatcat tcaagccagt gatttctact ggcgttgcag gtcctgcttc tttatcgcga
      181 cggccaagat cgtttgtcat tacacggaca cgtccgtaag tattaccgac aacgattggg
      241 tcaccaacgt gaagtgttcc ttcttggaca agtaaggttg acacaggacc tttaccttta
      301 tcaagacgtg cttcgatcac tgttccgatt gcgcgttgtg ttggatctgc ttttaaatct
      361 tctacttctg cgactaacag gatcatttcc agcaattctt cgatgttttg gccgaatttc
      421 gctgagatct caacaaaaat cgtttctcca ccccatgctt caggaattaa ttcatactca
      481 cttaattctt gcataacatg ctgaggattt gcacctggtt tatcaatttt atttacagcg
      541 acgatgattg gaacgcctgc agctttggca tggttgattg cttcaactgt ttgtggcatg
      601 acgccatcat ctgcagcaac aactaagatc gtgatatctg tgatacttgc tccacgtgca
      661 cgcatacttg taaaggccgc atgtcctggt gtatccaaga aagtgattgg tttaccatca
      721 atatcgatct ggtaggcacc gatatgctgt gtgattccgc cggcttcgcc gcttgttaca
      781 cgtgaatggc gcaatgtatc caataaggtt gttttcccgt ggtctacgtg tcccataatc
      841 gttac
//
LOCUS       DANKHJ010000334          843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000334 DANKHJ010000000
VERSION     DANKHJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.347"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>843)
                     /locus_tag="PW113_003580"
     rRNA            complement(<1..>843)
                     /locus_tag="PW113_003580"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DANKHJ010000335          842 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000335 DANKHJ010000000
VERSION     DANKHJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.348"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..618
                     /locus_tag="PW113_003581"
     CDS             <1..618
                     /locus_tag="PW113_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2527792.1"
                     /translation="RLLGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFT
                     YFQQVGGLPCKPVTSEITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSK
                     YSFEVSDQDLLLSNFDRFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTE
                     RAGYLSRIRNMARAVAKIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            620..>842
                     /locus_tag="PW113_003582"
     CDS             620..>842
                     /locus_tag="PW113_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2527793.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGP"
BASE COUNT          283 a          157 c          183 g          219 t
ORIGIN      
        1 cgtcttttag gaattgatcc attagaacac gatattcgtt ttgttgaaga caactgggag
       61 aacccatcga tggggtgtgc cggtgtgggt tgggaagtat ggcttgatgg gatggagatc
      121 acacaattca cgtacttcca gcaagtcgga ggactacctt gtaaaccagt tacttctgag
      181 atcacttatg gactagaacg tcttgcttca tatattcaag aagttgaaag tgtctatgat
      241 ctggaatggg ctgatggtgt gaaatatggc gagatcttca aacaaccaga atatgaacat
      301 tccaaatatt cttttgaagt aagtgaccaa gacttgctac taagcaactt tgaccgtttt
      361 gaaaaagaag ctaaacgctg tatcgatgaa aatcttgttc atccagcgta tgattatatt
      421 ttgaaatgca gccatacatt caatttatta gatgctcgcg gtgcagtatc cgtaacagaa
      481 cgagcaggtt atctttcccg tatccgcaac atggctcgtg ctgtagccaa aatctttgtg
      541 gcagaacgcg aaaaattagg attcccttta ttgaataaaa acacagcaaa tccagaaaac
      601 atgaaggagg ctgaataaga tggcaaaaga cttattactt gaaatcgggc tggaagaaat
      661 gcctgctcat gttgtcacac caagcatgaa acaattagaa gaaaaaacag caaaattttt
      721 agatgaaaac caattgactt atgatacgat cgaaacgttt tctacaccgc gccgcttagc
      781 aatcaaagta acaaacattc cagaacgtca agcagacagt gaagaagaag tcaaaggacc
      841 ag
//
LOCUS       DANKHJ010000336          829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000336 DANKHJ010000000
VERSION     DANKHJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.349"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          322 a          117 c          130 g          260 t
ORIGIN      
        1 atcttaattt tttaaattgt ctaaattata tagaaagaac aatctttcta tataattaga
       61 cataagagag actacggttg atttgatatt aacaagcgta tatgaatgta tatacaaata
      121 aaaaagggga gggagaagtt aaagaaaatt cagagaagtg gggcactcag tagtattaac
      181 aatttaaaag gaatttctta aagaaaatgg gttgcatcct ggagatacaa tttttttaga
      241 taaagaaaaa attcaagcaa caattcaaaa aaatcttaac tgctgagatt gatgtagcaa
      301 tatctcaatc atcaacaaag tacgatcctg cctttcaaaa gcttattgat aaatgtttat
      361 tcattataaa aataaaagaa aaatttatgt cagctttttt catcaaagtg gtaatgataa
      421 ttcgtctatt aattgtattt tttaggaaat atggtataat tgttttaacg aaaaggtaat
      481 tcatgataca ccaaaaacca ctagctggcg ggctagtggt tttcttttac gttctattca
      541 gattaacggg taggcttact gtcttttttg gcaataattt tgcaatcaag ccatttacag
      601 atgtaatgcg cggatacttc tgctaagatt gcaaaaaaca tatcgaaaag gtaattcata
      661 atacaacctc ctccccttgc cataataggc cggggtagca gacagcataa atattatacc
      721 ataactgaaa atttcatttt agtacatttc aatatattga tgaaccagca caataaaaca
      781 tgccatgact aacagaaaaa aacttattaa accagatata acttgtaaa
//
LOCUS       DANKHJ010000337          809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000337 DANKHJ010000000
VERSION     DANKHJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.350"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(174..344)
                     /locus_tag="PW113_003583"
     CDS             complement(174..344)
                     /locus_tag="PW113_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527794.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(356..622)
                     /locus_tag="PW113_003584"
     CDS             complement(356..622)
                     /locus_tag="PW113_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527795.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(633..>809)
                     /locus_tag="PW113_003585"
     CDS             complement(633..>809)
                     /locus_tag="PW113_003585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527796.1"
                     /translation="NGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHWSGPKA
                     NRTILEIIRDYRIR"
BASE COUNT          232 a          179 c          113 g          285 t
ORIGIN      
        1 cgtatctttt atagctctat tttcgtttgc tgttcctaaa acttttcaaa gcaaatctag
       61 caacgcaaag gttaatgtag aaaacactga tagaaaaaat gatgaaagtt attaaaaaaa
      121 ataattcttt catttattgt caaacctttc ttatagacat tagacttttt tagtcagtcc
      181 ctcccgactg gctttttcgc tctgtactca gcttcatcaa gcccaatgaa aatccaaagc
      241 atgtaaacga tcgtgccgat taatgcttgt ctactacccc atagacctaa agcgtagata
      301 attagtggcg cgctgaatac taatgttcga ttaaactttc ccatgttgac ctcctttaaa
      361 ttttgtattt agccatcagt tcatcgatgt cctttttgtc atatcttaaa aagccatcta
      421 tacgaatttc ttttaaaccg tgagctatta acttctcaaa accttgatcg tttacacctc
      481 ccacatattt tctagcttcg atctttttta aatatcgtgt ttctgacaaa ttcttttttg
      541 aaagttgact aagtgcgtcc tttacaactt caacaattgc aattcttaaa cgagtcaaaa
      601 actcttcgct tagaatattc atgacttgcc tcctatcgaa ttctgtaatc acggatgatc
      661 tctagaatag ttctgtttgc ctttggtcca ctccagtgtc catcaagaat ttgttgcatt
      721 cgtacacgcg tgtgaccata agcagtagcc aaatcatcca tagttattcc attctctttc
      781 ataaactctt tgatcgccgc acgaccgtt
//
LOCUS       DANKHJ010000338          800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000338 DANKHJ010000000
VERSION     DANKHJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.351"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..465
                     /locus_tag="PW113_003586"
     CDS             <1..465
                     /locus_tag="PW113_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2527797.1"
                     /translation="IQGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASV
                     AISSGTFEVYQANPQLSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDL
                     DIPFQQVINEKSFVLEYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            565..>800
                     /locus_tag="PW113_003587"
     CDS             565..>800
                     /locus_tag="PW113_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527798.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERE"
BASE COUNT          278 a          127 c          156 g          239 t
ORIGIN      
        1 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
       61 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
      121 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
      181 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
      241 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
      301 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
      361 atggctattc ttaccacatt gtccgcacta cttgttcttc ctttaacctg cgtgactaca
      421 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
      481 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
      541 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
      601 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
      661 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
      721 caggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
      781 gatccaaagg aaagagaaag
//
LOCUS       DANKHJ010000339          788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000339 DANKHJ010000000
VERSION     DANKHJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.352"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            55..720
                     /locus_tag="PW113_003588"
     CDS             55..720
                     /locus_tag="PW113_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527799.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT          276 a          155 c          141 g          216 t
ORIGIN      
        1 tgtataggtc atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac
       61 atcggagaaa aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc
      121 gaaaaaatcg gtgtttccga taaaacaatc tctagttggg aaaattctcg tacaatgcca
      181 gaccttgaaa tgctgtcact tttacatcag actttaggtc tgccgattga ttttccttct
      241 attgaatcag ctaccgaatc aatagaacca acattgccgg atgaacctct tgttatcaag
      301 aaatcttatt catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc
      361 gtcttttgtt tggtctatct ttttcttatc aattatcaat ataaaaatga aacggttgat
      421 cgattcaatc cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg
      481 gaaaattcca tcgaacattg ggtatgtaat gatatctttg ttcatggtac atggctgtct
      541 tttcacgctg gagaggtaga aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa
      601 cacgtcatga acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc
      661 ctcatccaag actatgacga tgccttaaaa agaagccctc aggagccaga taggcattaa
      721 gtaggagatg aacgtagaga tagaaaaaat caatgcacat gatcttatca aaaaaaaaca
      781 ggggtaca
//
LOCUS       DANKHJ010000340          779 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000340 DANKHJ010000000
VERSION     DANKHJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.353"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..372
                     /locus_tag="PW113_003589"
     CDS             <1..372
                     /locus_tag="PW113_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2527800.1"
                     /translation="GLIHINELKQDYFHFVENHLALVGERTGLTFKIGQKVRVKVVKA
                     DPDERAVDFELVSAEEVTALERPKTRNGKSGKREGKQAGKRPERNGKKNKRYSDSKQT
                     FGKKKGKKPFYKGVKKKKKKK"
     gene            394..>779
                     /gene="smpB"
                     /locus_tag="PW113_003590"
     CDS             394..>779
                     /gene="smpB"
                     /locus_tag="PW113_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2527801.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKS"
BASE COUNT          305 a          124 c          176 g          174 t
ORIGIN      
        1 ggattgatcc atatcaatga attgaaacag gactacttcc attttgttga aaatcattta
       61 gctcttgtag gtgaaagaac tggtctgact tttaaaatcg gacagaaagt gcgagtaaaa
      121 gtagtcaaag ctgatccaga tgaacgagca gtagattttg aacttgtttc cgcagaagag
      181 gttacagcgt tggaacggcc aaaaacacga aatggaaaat caggaaaacg cgaaggaaaa
      241 caagcaggaa aacgcccaga aagaaatggc aagaagaaca aacgatatag cgatagtaag
      301 caaacgttcg gtaagaaaaa agggaagaag ccattttaca aaggtgttaa aaagaagaaa
      361 aagaaaaaat aaaatgagtt ggaggaaaca gctatgccaa aaggcgaggg aaaattaatt
      421 gcacaaaaca agaaagctcg ccatgattat tcgatcatcg acacgatgga agcagggatg
      481 gtcttgcaag gaaccgagat caagtcgata cgaaacagcc ggatcaatct aaaagatgga
      541 tttattcgcg tccgcaacgg agaagctttc ttgcataatg ttcatatcag tccttatgaa
      601 caaggaaata tttttaatca tgatccgttg cgcacgagaa agttattatt acacaaaaaa
      661 caaatcatcc ggcttgaaaa tgaattgaaa aatactggaa tcactgttgt tcctttaaaa
      721 gtctatattc gtaacggcta tgccaaggta ttgattggtc tggcgaaagg gaaaaaatc
//
LOCUS       DANKHJ010000341          772 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000341 DANKHJ010000000
VERSION     DANKHJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.354"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            6..>772
                     /gene="bsh"
                     /locus_tag="PW113_003591"
     CDS             6..>772
                     /gene="bsh"
                     /locus_tag="PW113_003591"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2527802.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVE"
BASE COUNT          261 a          110 c          138 g          263 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa ca
//
LOCUS       DANKHJ010000342          769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000342 DANKHJ010000000
VERSION     DANKHJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.355"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            1..459
                     /locus_tag="PW113_003592"
     CDS             1..459
                     /locus_tag="PW113_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2527803.1"
                     /translation="MLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            468..695
                     /locus_tag="PW113_003593"
     CDS             468..695
                     /locus_tag="PW113_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527804.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          252 a          120 c          142 g          255 t
ORIGIN      
        1 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
       61 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      121 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      181 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      241 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      301 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
      361 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
      421 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
      481 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
      541 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
      601 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
      661 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
      721 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacg
//
LOCUS       DANKHJ010000343          757 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000343 DANKHJ010000000
VERSION     DANKHJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.356"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..485
                     /locus_tag="PW113_003594"
     CDS             <1..485
                     /locus_tag="PW113_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527805.1"
                     /translation="AVLSRIILGPKPPRPEITRGEFDFRLEYEVDGERIVIEDTIVAL
                     FDGFSADAGSMAWYRTWRLHLASDRRSRNILLDELEDGRRIYYVPESANYSMGDVQKK
                     REPNPNWYPFNGVTIEYPRNKTPEIGAKFISGLEDLYDRFGIRLISWEHDPPIENRFE
                     "
BASE COUNT          268 a          104 c          168 g          217 t
ORIGIN      
        1 atgcggtttt aagtagaata atattgggtc ctaagccacc aagaccagaa attacccgag
       61 gggagtttga ttttagatta gaatatgaag tagatgggga aaggattgtg atagaagata
      121 caattgttgc attatttgat ggattttctg cagatgcagg tagcatggca tggtatcgca
      181 cgtggcgtct acatttagca agtgatagac gatcacgaaa tatcttatta gatgaattag
      241 aggatggtcg aagaatatat tatgtcccag aaagtgctaa ctattctatg ggagatgtac
      301 agaaaaaaag agagcctaat cctaattggt atccatttaa tggcgtaaca atagaatatc
      361 cacgcaataa aacacctgaa ataggtgcaa aatttataag tggtttagaa gatctgtacg
      421 ataggtttgg aatccgtctt attagttggg aacacgatcc accaatagaa aatcgctttg
      481 aataatcgaa atctactaat ccatttcatc agccagtgaa tgttgtaatg gaacaatttg
      541 gtttagtgtt caaaaccatt gaaatactag gctgtgatca gcaaaaatga atctgaataa
      601 aaaagagagc aactattagc tgagaggagt taatagttgt tagctttata cgaatcaaag
      661 tggcaaatac agatgcgtat gcatttgtgt ttgccatctt gcgtttggta gacgagatag
      721 gtataagcta aagaaacaac ttgtttaaaa taaacga
//
LOCUS       DANKHJ010000344          745 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000344 DANKHJ010000000
VERSION     DANKHJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.357"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..143)
                     /locus_tag="PW113_003595"
     CDS             complement(<1..143)
                     /locus_tag="PW113_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HDL2527806.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSG"
     gene            complement(158..>745)
                     /locus_tag="PW113_003596"
     CDS             complement(158..>745)
                     /locus_tag="PW113_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2527807.1"
                     /translation="KHDITTITAASTDAKAAKYVQELFMNNYFRIYTNPDVIGVEMGA
                     ALKNIIAIGAGAIHGLGFGDNAKAAIMTRGLAEISRLGVAMGANPLTFIGLSGVGDLV
                     VTCTSVHSRNWRAGNLLGQGQPLEEVLANMGMVVEGVNTTQAAMELAQIMEIEMPITQ
                     TIYEVLYEQKEIKTAAKEIMLRDGKKENEFSLTIF"
BASE COUNT          177 a          180 c          134 g          254 t
ORIGIN      
        1 cctgatttca acgcttcttc aacgatgaac tggattgttg gtttatcaac aattggcaac
       61 atttcctttg ccatagcttt tgttgctggt aaaaatcttg ttcctaatcc tgccgctgga
      121 ataactgctt ttttgacttt catatacatt cgctccttca aaaaattgtt aatgagaatt
      181 cattttcttt tttcccatca cgtaacatga tttcttttgc ggctgttttg atttcttttt
      241 gttcatacag cacttcatag attgtttgag tgatgggcat ttcgatttcc attatttggg
      301 ctagttccat cgctgcttga gtagtattca caccttcaac gaccattccc atattggcta
      361 gcacctcttc taggggctgc ccttgtccta acaagttgcc tgcgcgccag ttcctagaat
      421 gaacacttgt gcaagtaacg actagatcgc ctactccgct taatccgata aaggtcagcg
      481 ggttggcacc cattgctaca cctaaacggc tgatctcagc taatccgcgc gtcatgattg
      541 ctgcctttgc attatcgcca aaccctaagc cgtgaatagc gccagcacca atagcaataa
      601 tatttttcaa agctgccccc atctctacac cgatcacatc aggattggta tagatccgaa
      661 aataattatt catgaacagc tcttgtacat attttgctgc ttttgcatct gtacttgcag
      721 ctgtgatcgt agtgatgtca tgttt
//
LOCUS       DANKHJ010000345          745 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000345 DANKHJ010000000
VERSION     DANKHJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.358"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>745
                     /locus_tag="PW113_003597"
     CDS             <1..>745
                     /locus_tag="PW113_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2527808.1"
                     /translation="VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNS
                     QWMIGGDGWAYDIGFGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFS
                     ASGKYASKKDLGMMAMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCI
                     THGLLGGMKESLKEAKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMR
                     KQVRFSSLDNANPNLAKALFEKTVHDAKTR"
BASE COUNT          244 a          145 c          166 g          190 t
ORIGIN      
        1 gtacacatgt ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc
       61 gaaagcgaac gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc
      121 gtaaaaaaca gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc
      181 gggatcgatc atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa
      241 gtctattcca atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa
      301 ttttcggcta gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat
      361 ggaaatgttt atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca
      421 tttgaagaag cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc
      481 acacatggct tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct
      541 tctggttatt ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca
      601 atgactttgg actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa
      661 gttcgctttt cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag
      721 actgtccacg acgctaaaac acgtt
//
LOCUS       DANKHJ010000346          737 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000346 DANKHJ010000000
VERSION     DANKHJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.359"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..320
                     /locus_tag="PW113_003598"
     CDS             <1..320
                     /locus_tag="PW113_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527809.1"
                     /translation="AKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTEPNLDSL
                     TALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQENERNQL
                     LKD"
     gene            complement(396..>737)
                     /locus_tag="PW113_003599"
     CDS             complement(396..>737)
                     /locus_tag="PW113_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527810.1"
                     /translation="TYLMTEKELTNTVCERLRNVGNNSVENALKENRGYFVKQGFTLE
                     RASKDIKRLYNVKASSSFLVYLAKGAGIDEVIKELILSGVELVPLYQFTSVTRDDGRR
                     VRKRKDPIVLN"
BASE COUNT          234 a          144 c          126 g          233 t
ORIGIN      
        1 atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg
       61 aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta acagagccta
      121 acctcgatag cttaaccgct ctttctagag ccctaggaac ccctgtaagc cattttctag
      181 acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg
      241 aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca
      301 atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg
      361 agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt
      421 ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata aagaggaact
      481 aattcaactc ctgataatat caattctttt attacttcat ctatccctgc tccttttgct
      541 aggtagacta aaaaactact acttgctttc acattgtata gtctcttaat atccttagaa
      601 gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttcttt taaagcattt
      661 tccacgctgt tattcccaac attacgtaga cgttcacaaa cagtgttagt aagttctttc
      721 tctgtcatta gatatgt
//
LOCUS       DANKHJ010000347          724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000347 DANKHJ010000000
VERSION     DANKHJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.360"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(546..>724)
                     /locus_tag="PW113_003600"
     CDS             complement(546..>724)
                     /locus_tag="PW113_003600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2527811.1"
                     /translation="EMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNG
                     VLRCTAKGMGRLIS"
BASE COUNT          215 a          162 c          105 g          242 t
ORIGIN      
        1 gttcatattt tatcgttttt cgtttactta aaaatacata aaaaatatcg actcgtttat
       61 ggcgtccgaa acgagaaacc ctagtagaaa tcggtaaatg ctcgattgat ccaatgacca
      121 acgtcctcgc aagtatctac atgtactgca gcacacgaca caatttcttg tgcgtcctcc
      181 ccattacgag aagacctact ccttattcca tgagtttgcg tcaaagccta gatcccttag
      241 tagctgctca tgattaatct aggcgctatt ctaacttgtc taacttctaa attccctctt
      301 gtttactctg tgcatattat ttacacaaat tcatttcacc actatccaaa caagggcatt
      361 tactcgattg accaattttt tacgttacca atcgtaaaaa cctaccccgt caccccccga
      421 aaggaattgt tgatagatca agtctactcc ttatttttct agtaattcta agttctttta
      481 gaagtctcga caatattttc aagcaagcga aaaacgataa aatacaagtt atgagaatga
      541 tcaattcagc tgattaaacg ccccatccct tttgcggtac aacgcaagac tccatttttt
      601 cttcgaccaa tcagtacacc gttttggtca taaacatgtt tgattgttgg atatagttct
      661 tttaaaggga taggatcttt taaaattatt tgcttttgtt taccagcgta ataaatcatt
      721 tcct
//
LOCUS       DANKHJ010000348          723 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000348 DANKHJ010000000
VERSION     DANKHJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.361"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..66
                     /locus_tag="PW113_003601"
     CDS             <1..66
                     /locus_tag="PW113_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HDL2527812.1"
                     /translation="GRKIFEKTKERFHDFQKTASS"
     gene            complement(152..>723)
                     /locus_tag="PW113_003602"
     CDS             complement(152..>723)
                     /locus_tag="PW113_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2527813.1"
                     /translation="FTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAALQNGTD
                     STGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNAVIRNS
                     ATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGRQSVEL
                     FVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
BASE COUNT          179 a          156 c          144 g          244 t
ORIGIN      
        1 ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa aactgcttcg
       61 agttgatgaa taagagctga gacgtaagca tagcagattt cagggagttg gccttgaatc
      121 ggctccactc cctgaagtca ttgatttgat tttacgcttt cccttctgta ggagcatctg
      181 ttaacgacca tgtgaatgtt gaaacgtagt tgtctacggc ataattggat acaggaacaa
      241 ataattccac tgattgtctc ccttgttcca cattctctcc taatgaataa atccatgtgc
      301 caatcccttc atttgctcct gcagttacta gatctactgc ctgatctgct gggatctcaa
      361 ttccttcagc gccagtacca gaaatagtag atggcgttcc actgctatta gttgcgctat
      421 ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt tagtttattc gtatcagatg
      481 ttgtaccaat aaattctgtt cgtttgactg atagtttcca accggcataa attccgcttt
      541 tatccgttac ttgtacgaaa ttctgtactt gtgttgctgt ccctgtactg tccgttccgt
      601 tttgtaatgc agcatatact gtttttgctg tacctaactt gattgttcca aaatcaagat
      661 ctggagcttg gtcaatcgtc agtaatccgg atgttccctg atttggttcg ttaggtgtaa
      721 acg
//
LOCUS       DANKHJ010000349          719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000349 DANKHJ010000000
VERSION     DANKHJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.362"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..135
                     /locus_tag="PW113_003603"
     CDS             <1..135
                     /locus_tag="PW113_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2527814.1"
                     /translation="RTKDPIEAFKWGVACGSATAFSDDLAARELIDELLPEVEITKID
                     "
     gene            153..>719
                     /locus_tag="PW113_003604"
     CDS             153..>719
                     /locus_tag="PW113_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2527815.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAH"
BASE COUNT          256 a          140 c          163 g          160 t
ORIGIN      
        1 cgtacgaaag atccaatcga agcctttaaa tggggagttg cttgcggaag cgcaacagct
       61 ttttctgatg acttggcagc tcgcgaattg attgatgagt tgctgccaga agttgaaatc
      121 acaaaaattg attagtttca aggaggataa tcatgaatat caaggattta atggtcaaag
      181 aagcgatgat catggatttg caagcgactg ataaaaaagg cgcaatcgat gaaatggtcc
      241 aaaaaatgtt tgatgccggc cggatttccg atatcgaaac atataaagaa ggaattctag
      301 cccgcgaagc acaaacttct acaggtcttg gtgatggaat cgccatgcct cacgcgaaaa
      361 acagtgcagt taaagaagca acagttttat tcgcaaaaag ccaaaaagga gtagattatg
      421 aagccttaga cggacagcca acttatctgt ttttcatgat cgcagctcca gaaggcgcaa
      481 acgacacaca tttgcaagca ttagcagcac tatctcgatt gctgattgat gcagatttcg
      541 taggaaaatt aaaagaagca aatacaccag aagaagtcca agacttgttc caaacagctg
      601 aagagcaaaa agaagcagaa gaaaaagcag aacaagaaac acaagctgca gaagcttctg
      661 ctcatccaga aagaaaattc gttgttgccg ttacagcttg tccaacaggg atcgctcac
//
LOCUS       DANKHJ010000350          715 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000350 DANKHJ010000000
VERSION     DANKHJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.363"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>715
                     /locus_tag="PW113_003605"
     CDS             <1..>715
                     /locus_tag="PW113_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2527816.1"
                     /translation="TAKWTETKWANVNRITFQDEFSSFNSGVAKLRKGQTATIKAKID
                     YSGDLAPILQSAVTFENVDPALSIGYDENTLTIQHKQAIFTFTVTLNQDLLKPALFTV
                     KVSDGLPNDHHHLTPYSQRQTIEQDSAIDSGGDLVEEPTDKPENENKPEVPPTENPDG
                     EQKPEIEPGEEPDTETQPEPDNESKPEITPGEKPDVDPEEKPDVTPEPDTDSGNQTVP
                     ETNPDTDNETENPEKPEVAP"
BASE COUNT          281 a          162 c          137 g          135 t
ORIGIN      
        1 acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa
       61 ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa
      121 gcaaaaattg actattcagg agacttagca cctatactac aatcagctgt tacatttgag
      181 aatgttgatc ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag
      241 caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt
      301 actgtcaagg tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag
      361 cggcagacaa ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca
      421 acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga
      481 gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca accagagcca
      541 gacaatgaat caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa
      601 aaaccagatg tgactccaga accagatacg gattctggaa accaaacagt accggaaaca
      661 aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtagc cccag
//
LOCUS       DANKHJ010000351          707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000351 DANKHJ010000000
VERSION     DANKHJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.364"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>707
                     /locus_tag="PW113_003606"
     CDS             <1..>707
                     /locus_tag="PW113_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2527817.1"
                     /translation="QKWSIDTNILDDGKHIVPEIIESINHRLNLYNMKAEDFIGIGMG
                     TPGSVDSEAGTVIGAYNLNWTEVQFVKKLIEAGTGIKFAIDNDANVAALGERWKGAGE
                     NDPDVVFVTLGTGVGGGIVAGGNLIHGVSGAGGEIGHITVDPEGFECTCGKRGCLETV
                     SSATGVVRLGRFLAEEYAGDSKLKAMLDNGEEVTSKDIFEMAQEDDPFALMVVDRVCF
                     YLGLACGNLGNTLNPSS"
BASE COUNT          216 a          115 c          192 g          184 t
ORIGIN      
        1 caaaaatgga gcatcgatac caatattttg gatgatggta agcatatcgt tcctgaaatc
       61 attgaatcaa tcaatcatcg tttgaattta tataatatga aagccgaaga ctttatcgga
      121 atcggcatgg gtaccccagg aagcgtggac agcgaagctg gaacggttat tggtgcgtat
      181 aacttaaact ggacagaagt tcaatttgtg aaaaaactga tcgaggcagg aacaggaatc
      241 aaattcgcta tcgataatga cgcgaatgtt gctgctttag gtgaacgatg gaaaggtgct
      301 ggagaaaacg atccagacgt tgtattcgtt actctaggaa caggtgttgg cggaggtatc
      361 gttgctggcg gaaatttgat tcacggcgtg agcggagctg gaggagaaat cggacatatc
      421 actgtggatc ctgaagggtt cgaatgtact tgcggtaaac gcggatgttt agaaactgta
      481 tctagcgcta caggtgtggt acgcctaggt cgtttcttgg cagaagaata cgcaggggat
      541 tcaaaactga aagcaatgct agacaacgga gaagaagtaa caagtaaaga tatctttgaa
      601 atggctcaag aagacgatcc gtttgcattg atggttgttg atcgcgtatg cttctactta
      661 ggtttagctt gcggaaactt ggggaataca ttaaatccgt caagtat
//
LOCUS       DANKHJ010000352          696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000352 DANKHJ010000000
VERSION     DANKHJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.365"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(54..629)
                     /locus_tag="PW113_003607"
     CDS             complement(54..629)
                     /locus_tag="PW113_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2527818.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DANKHJ010000353          679 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000353 DANKHJ010000000
VERSION     DANKHJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.366"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>679
                     /gene="polC"
                     /locus_tag="PW113_003608"
     CDS             <1..>679
                     /gene="polC"
                     /locus_tag="PW113_003608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2527819.1"
                     /translation="FAELLQISGLSHGTDVWLGNAEELIRRGDATLAEVIGCRDDIMV
                     YLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDWYIDSCSKIKYM
                     FPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKGKDAVKQAMKEI
                     TDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIEGDTLIAPFRAV
                     PSLGTNVA"
BASE COUNT          205 a          115 c          162 g          197 t
ORIGIN      
        1 cttttgcaga actactgcaa atttccggac tatctcatgg tacagacgta tggttaggaa
       61 atgcggaaga attgattcga cgaggagatg cgacactggc ggaagtaatc ggctgtcgtg
      121 atgatattat ggtttatttg atccatgctg gattagatag cgggatggca tttaaaatca
      181 tggaaactgt tcgtaaagga caatggaaca agatccctga tgaattgcga gaaacctatc
      241 tatcggcgat gaaagaaaat aatgtgcccg actggtatat cgattcttgt tcaaaaatca
      301 aatatatgtt tccgaaagcc catgctgcag cttacgtatt gatggctctt cgtgtcgctt
      361 atttcaaggt ttatttccct attttgtatt actgtgcata cttttctgta cgtgcagacg
      421 actttgatct agtctccatg tgcaaaggaa aagatgctgt gaaacaagca atgaaagaaa
      481 tcacggacaa gggattagat gcttccgtaa aagaaaagaa tcaactgaca gtgctggagt
      541 tagccaatga aatgctggaa cgaggattca aatttggaat gattgatttg tataaatcgg
      601 atgccgttaa ctttgttatt gaaggtgata cgttgatcgc accatttaga gctgtcccaa
      661 gtttgggaac caacgtggc
//
LOCUS       DANKHJ010000354          678 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000354 DANKHJ010000000
VERSION     DANKHJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.367"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          240 a          113 c           80 g          245 t
ORIGIN      
        1 caaattactt catcaaaaca ttgatataat ttttattata tcagtattta ttcttgatac
       61 ataaatgaga aaataaatac tttaaatcta ttgactacaa gtagtcatta agtcttcata
      121 agatgtacaa tatgacatta aaaaaataaa agcttacgac catccgttaa atctgacact
      181 aaataatgga ctgctttctt ttgtattagc aggttacgtt gactatgaat attcaccaga
      241 ttatttggag aaacctcttc aacacgagaa caatgatatt aattttagat ttgtctacga
      301 gtcaaaaagt aaaacatggt tagttgatca attaagcacg ataaacacaa gcttccatga
      361 attacctaaa cctgaaaatg aagttgagat ggtcattaaa atcttctaaa aaagaagcgt
      421 tgaaggggaa atattccttt caacgctctt ttttatatgc ttagaaacat aaataaattg
      481 tatactttct ttttagcccc tattactttt cacgaaaaat atcttttttt cgacgctagc
      541 gtttctctac ttctaatact gtacacagtt tttgtattat tgaaaaatta ctcattattt
      601 agcattaatc cacatcgttt tatcaatcga ccttcatact aaactacttt aaagtcatct
      661 tccttctcgt ttttcatt
//
LOCUS       DANKHJ010000355          675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000355 DANKHJ010000000
VERSION     DANKHJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.368"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..608
                     /locus_tag="PW113_003609"
     CDS             <1..608
                     /locus_tag="PW113_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527820.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
BASE COUNT          240 a          117 c          146 g          172 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tactt
//
LOCUS       DANKHJ010000356          655 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000356 DANKHJ010000000
VERSION     DANKHJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.369"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>655)
                     /locus_tag="PW113_003610"
     CDS             complement(<1..>655)
                     /locus_tag="PW113_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2527821.1"
                     /translation="GLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSR
                     RGLPYYPRGRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKK
                     IIEEYHLPIPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEAS
                     RIFIEGQIKEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFT"
BASE COUNT          174 a          154 c          119 g          208 t
ORIGIN      
        1 tgagtaaacc atccccatag tatttctttg tattccttag cagaaaaaac gtgccattca
       61 atagcctgcc ggattggatc acgccagtct tttaatgcat caaatgttga agcaaatgct
      121 cctgtgcaat tgcctttttc cgcttctttt atctgccctt caataaatat tcggctagct
      181 tctgaaaagg gctcattctg ataggcaaga ggagtttcta aatacgacca atcccatcta
      241 taagggatta gtgctggatg ctttccgaga cactcctctg aagagaggga gagaaaatca
      301 tgctcaaaat ttttctttgg aatcggcaag tgatattctt cgatgatttt cttgtaataa
      361 aacaactcaa cgtctttttt cagatagtca aaaaatgttt cgccagtcaa aaatcccgac
      421 tgatgccatt gctccaggtt ttcttttgtt agtaaccgtg gccatgccat tgctccgcct
      481 tgcttttgat ttcttcctcg agggtaataa gggagccccc ttcttgatcc actgtataca
      541 atcggttcct tgcctgatgg atgatagacc agttttccct tttcgttctc gaagcgccct
      601 cctcttgata ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag acctc
//
LOCUS       DANKHJ010000357          634 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000357 DANKHJ010000000
VERSION     DANKHJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.370"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>634
                     /locus_tag="PW113_003611"
     CDS             <1..>634
                     /locus_tag="PW113_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL2527822.1"
                     /translation="ASKIGVLVKGSNYLELLAQTETVVFDKTGTLTKGDFSIQTIDTK
                     IDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLPTTNIQEFAGEGILAEVDGKQVLA
                     GNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYLVIADTLKEDAVDALVQLKQAGVK
                     NTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKVQRLEEILQRNNKKTAF"
BASE COUNT          217 a          108 c          142 g          167 t
ORIGIN      
        1 gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact tggagttgct tgcgcaaacc
       61 gagacagtgg tattcgataa aaccggcaca ttaacgaaag gtgacttttc gatccagacg
      121 atcgatacaa aaatcgatcc aaagaaattc atgcaatata ttgcgagtgc agaacagttt
      181 tctacacatc cgattgctca atctgtttta gaaggctacg caggtccgct tttaccaaca
      241 actaatatcc aggaatttgc aggtgaaggg atcttagctg aagttgatgg caaacaagta
      301 ttagcaggta atcacaaatt gatggaaaga tttgaaatct cttttccttt aagtcaagaa
      361 atcggtacat tgctttatct agcgatcgat caaagttaca gtggttatct tgttattgca
      421 gataccttga aagaagatgc cgtagacgca ttggttcaat taaaacaggc tggtgtgaag
      481 aacacagtca tgttgacggg cgactcgaaa aaaattgctg atcatatcgg gaagcaagta
      541 ggcgtggaca agatatatag cgaattgctt cctgaagata aagtgcagcg gttagaggaa
      601 atcttacaaa gaaacaataa aaagaccgca tttg
//
LOCUS       DANKHJ010000358          631 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000358 DANKHJ010000000
VERSION     DANKHJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.371"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>631
                     /locus_tag="PW113_003612"
     CDS             <1..>631
                     /locus_tag="PW113_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HDL2527823.1"
                     /translation="DADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKV
                     DVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPN
                     AEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSN
                     EKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFT"
BASE COUNT          265 a           96 c          131 g          139 t
ORIGIN      
        1 gatgccgata aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca
       61 gacgcatctg agggaattaa gaacaaagaa gatctaccaa aaggaactac ttatacttgg
      121 aaagagaaag tagatgttag tacagctgga aacaaaaaag ggattgtagt agtaacatat
      181 ccagatggat ctaaagaaga agtagaggtt actatttctg tagaagataa aaaagcaccg
      241 aataaacctc aagttgatcc tattacagag ggtgaccaaa ttgttactgg taaaactgaa
      301 ccaaatgcag aggtgacagt tacattacca aacgggagtc aataccatgg gacagctgat
      361 aaaaatggta attttacagt taaagttcct aaattagagg caggaacaaa agtgatagta
      421 actgcaactg atgaatctgg taatactagt gaacctacta atgtagttgt ttcttcaaac
      481 gaaaaagaca gtgaaaaagc cgttagtaaa gataataaga ctgacaatca aggtagcaag
      541 caaaacaaaa atagaggcaa aagcagtcct caaaagcagt cctcaaaagc ttatcctaag
      601 acgggagaga ttgatagtaa tatctttact a
//
LOCUS       DANKHJ010000359          627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000359 DANKHJ010000000
VERSION     DANKHJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.372"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            3..269
                     /locus_tag="PW113_003613"
     CDS             3..269
                     /locus_tag="PW113_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2527824.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            296..595
                     /locus_tag="PW113_003614"
     CDS             296..595
                     /locus_tag="PW113_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL2527825.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
BASE COUNT          211 a           89 c          147 g          180 t
ORIGIN      
        1 taatggacag tttgccggtg gaattacaag ctttatgttg gaatctaatc gatcaaaacg
       61 ttcaaaagca atttccttta gactatcttc aaatttttga attttctacg gaaaaaggaa
      121 atcaaaagtt ggttcatcgt caagaagaac cggaagaaag aaaagagtat ctgatttcac
      181 ctaagcttcg tttgaaatca gtgtctcaaa aaatatgggc cattgattcg ggtgagtatc
      241 aaacgatgtt attaccagaa gaatactagt atctaaaaga aaggaacgaa aggacttgtt
      301 agaaacggct atcgaagtac tggaaaagtg tgcacagctg gtcactgctt ctgaagagtg
      361 gggctatgaa tccgttacga tggaaaaaga ggaaattgaa atgggtatgc ttcctgaaga
      421 tgttcatcta ccacgtttag tgatgacaca tctttatatt tattgcgcac cagaagatgg
      481 aaaagactat gtagtttatt tcattacaga tattaccagc cagcgagaat ttgttcgtgg
      541 gttactggta gaaggacgat tggtttggag tcagattgga ggaacggatg aataagatcg
      601 attatttgag aatgattgcg aaatgtt
//
LOCUS       DANKHJ010000360          625 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000360 DANKHJ010000000
VERSION     DANKHJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.373"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            1..625
                     /locus_tag="PW113_003615"
                     /pseudo
     CDS             1..625
                     /locus_tag="PW113_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DANKHJ010000361          624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000361 DANKHJ010000000
VERSION     DANKHJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.374"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..579
                     /locus_tag="PW113_003616"
     CDS             <1..579
                     /locus_tag="PW113_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527826.1"
                     /translation="FSLLAFTGIRKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLI
                     VNTPKSKSGKRKIYLDNDLISLLRKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSW
                     LNVIYKHHPELTKRITTHGFRHTHASLLFESGASLKDVQERLGHADIQTTSNIYTHVT
                     ETQNKKVINNFVAFMKNSTQGESKGESKNILG"
BASE COUNT          234 a          108 c          102 g          180 t
ORIGIN      
        1 ttttccctat tggcttttac aggtataaga aaaggagaag cttttgctct gacctggtcg
       61 gatattgact ttaagagcaa aacactcaat atcaataaaa ctgtaacaag aggttatcaa
      121 ggaagattga tcgtaaatac tcctaaatca aaatctggaa aaagaaaaat ttatctggac
      181 aatgatttga tcagtttatt gagaaagtat tatacaaaaa acaaaacgat cgttacaatc
      241 caaagggaaa acttaatttt tcaccatgat ggtcttctct ataaccctac agtttcacgt
      301 tcgtggctta atgtcatata taagcatcat ccagaattga ctaaaagaat cactactcat
      361 ggttttcgac atactcatgc ctctctcctt tttgaatcag gagcttcttt aaaagatgtt
      421 caggaacgtt tagggcatgc agacattcaa actacttcaa atatttacac tcatgtaact
      481 gaaactcaaa acaaaaaagt catcaataat tttgtagcat ttatgaagaa cagtactcag
      541 ggggagtcaa aaggggagtc aaaaaacatt ttaggctaaa tatacaacaa aaaagacacg
      601 atcgaaatcg tgtctaatcg ttga
//
LOCUS       DANKHJ010000362          611 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000362 DANKHJ010000000
VERSION     DANKHJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.375"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>611
                     /locus_tag="PW113_003617"
     CDS             <1..>611
                     /locus_tag="PW113_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2527827.1"
                     /translation="WGIQPLILKKIGGRPTNEILGTGIGAVLVGLLVQLFASPAGISV
                     TTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSLIGVFAFGEWSGS
                     MGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGYWVYSALPKLVDA
                     SGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAI"
BASE COUNT          160 a          105 c          145 g          201 t
ORIGIN      
        1 tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa tgagatatta
       61 ggaactggta ttggagcggt gcttgtcggc ttactcgttc aattattcgc ttctccagct
      121 ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattttg ggttattgga
      181 caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat gccaatttcg
      241 actgcattac agttagtcgg aacctcgttg attggtgtct tcgcttttgg agaatggtct
      301 ggaagtatgg ggaaaatcat tggagcagca gcaatcattc ttcttgtatt tggttctgct
      361 ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg aattagtcta
      421 ttagcatcaa caagtattgg ttattgggta tacagtgcat tgcctaaact agttgatgca
      481 agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc agtgatttat
      541 gtgattttcc gtaaaccaac cgcattttct gaagacaaga gttggaaagc aacgatcgtt
      601 ggagccatct t
//
LOCUS       DANKHJ010000363          610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000363 DANKHJ010000000
VERSION     DANKHJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.376"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            61..>610
                     /locus_tag="PW113_003618"
     CDS             61..>610
                     /locus_tag="PW113_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2527828.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQL"
BASE COUNT          226 a           96 c          132 g          156 t
ORIGIN      
        1 tcattcggct gttggaaaga gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa
       61 atgaaaaaaa aaggaaaagc aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta
      121 ttagtaggac tagctttgat tttcaatgaa cagatcaagg attatcttgt cagagagaca
      181 ggagataaat atgctatagc gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa
      241 gatgtcagtt ttgactttga tgcggtagaa ccaatgacga cagaaggggt catgaggtcg
      301 cagatgagag gaacagatct accagtgatt gctagtatcg cagtcccttc agtttcaatc
      361 aatttaccga ttttcaaagg cttggataat acgtcactac tttacggagc aggaacatta
      421 tctccagatc aagaaatggg aaaaggaaat tatgccttag caagccatcg tgcaacgaac
      481 cctgaattgt tgtttacacc gctggaaaat ttagagatgg gtgccaagat ctatttgaca
      541 gatttagaaa atgtgtatac atacaaaaca ttttttaaag aaaaagttgc tcctacagat
      601 acccaattac
//
LOCUS       DANKHJ010000364          609 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000364 DANKHJ010000000
VERSION     DANKHJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.377"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..485
                     /locus_tag="PW113_003619"
     CDS             <1..485
                     /locus_tag="PW113_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2527829.1"
                     /translation="FNKNTVRLKYALDKYQRKTHTKGWKDTVIQAPEVVMTLLREWKS
                     AQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRINAIKKRHPELAQLSPH
                     KLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVVDKSVFEAFQKGLNKCD
                     "
BASE COUNT          231 a          103 c           97 g          178 t
ORIGIN      
        1 attttaataa aaatacggtt cgtctcaaat atgctttaga taaatatcaa agaaagacac
       61 atacaaaagg ttggaaagac accgttattc aggcaccaga agttgtgatg acattattaa
      121 gagaatggaa atcagctcag gctgaacaat tgttgaaact aaaaattata caaacacctg
      181 accagtacct ctttacctat tccaaacctt ctggagaagt aaattgtcct gttcacgctg
      241 actacttaaa ctatcgaatc aatgcaataa aaaaaaggca tcctgaattg gctcaattat
      301 ctccacacaa acttcgtcat acctatgcaa ctatagctag ggaaggtgga gcaaatatga
      361 atcaaatctc taatgcttta acgcactcgg atatttctac tacaaaaata tacgtgaaca
      421 cacctgacgt agttgataaa tctgtttttg aagcatttca aaaaggattg aataagtgtg
      481 attaacatgt gttctcaaat gtgttcttat tataaaaaaa gacactttcc aaataattga
      541 aaagtgtcct gaaatattga tataaaaaga atattaacgt tttgagaatt gtgaagcttt
      601 acgagcttt
//
LOCUS       DANKHJ010000365          609 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000365 DANKHJ010000000
VERSION     DANKHJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.378"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..308)
                     /locus_tag="PW113_003620"
     CDS             complement(<1..308)
                     /locus_tag="PW113_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL2527830.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     "
     gene            394..>609
                     /locus_tag="PW113_003621"
     CDS             394..>609
                     /locus_tag="PW113_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2527831.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIV"
BASE COUNT          164 a          135 c          112 g          198 t
ORIGIN      
        1 tagattttcc ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt
       61 tccactgcat ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac
      121 cctaacccta gtgcagcaat cattgttgcg cctaatgcag gtccttcctc cacacttaaa
      181 caatagaccg ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg
      241 cctcctccca ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat
      301 cccaccattg acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat
      361 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
      421 gcgatcaaag acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt
      481 atcgttggga aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc
      541 tatgctggtt ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt
      601 tccatcgtt
//
LOCUS       DANKHJ010000366          602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000366 DANKHJ010000000
VERSION     DANKHJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.379"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>602
                     /locus_tag="PW113_003622"
     CDS             <1..>602
                     /locus_tag="PW113_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HDL2527832.1"
                     /translation="ETTDTQTAPNGVITFEKSLDEIFKEIGIDNLKTDRLMYRIYLVS
                     HQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVSAKYEEPNTQTKDGS
                     GSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVPYVAGIQMHMVQADK
                     VTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKL"
BASE COUNT          217 a           92 c          118 g          175 t
ORIGIN      
        1 gaaacaactg atacacaaac tgctcctaat ggggtaatta cgtttgaaaa gagcttagat
       61 gaaattttta aagagatagg catagacaat ctcaagaccg atcgtttaat gtatcgaatc
      121 tacttggtaa gtcatcaaga cgatgataaa attgtaccag gtattgatag tacgggctac
      181 tttttaacag attcagatga tttctataat agcttagatg tatctgaaaa taatcctgac
      241 cagtttaaac atggatcagt aagtgcaaaa tatgaggaac ctaatactca aacaaaagat
      301 ggttcaggat ccacaggtgc taatggagct ataatcttag atcataagtt aacgaaaaat
      361 tataatttct catattcagc ttccgcaaag ggcacgcctt ggtatgctaa ttacaaaatt
      421 gatgaacgat tagttccata tgtagcgggt atacagatgc acatggttca ggctgacaaa
      481 gtaacatatg atgtttcttt tgaatcaggg aaaaaagtgg ctgatttagc aattgaacgg
      541 cgcaaagatc atgaaaatta tggtgtaggt tcaattactg ataatgatct taccaaactt
      601 at
//
LOCUS       DANKHJ010000367          595 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000367 DANKHJ010000000
VERSION     DANKHJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.380"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>595
                     /locus_tag="PW113_003623"
     CDS             <1..>595
                     /locus_tag="PW113_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2527833.1"
                     /translation="FNKVFLEGQPVDEYVEMLLNEMKIMLESHPTANLETLYVGGGTP
                     TSLSAKQLDRLLSGAREILPFQNGNEFTVEANPGDLTREKLQVMKNYGVNRLSMGVQT
                     FDNRLLKKIGRKHTAEDVYQTMQFLEAENFTNVSIDLIYALPGQTLEGYLDTLEKALA
                     LDLPHYSLYSLILENKTMFMNWVRQGRLELPDQETETR"
BASE COUNT          204 a          102 c          127 g          162 t
ORIGIN      
        1 tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat gctgctgaat
       61 gaaatgaaaa taatgctaga aagtcatccg acagcgaatt tagaaacact ttacgtaggg
      121 ggcgggacac caacttcttt atctgctaaa caacttgatc gactgttatc aggtgctaga
      181 gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc aggtgatttg
      241 acccgtgaaa aacttcaagt aatgaaaaac tatggcgtca atcgtttatc catgggagtg
      301 cagacatttg ataatcgctt attgaaaaaa attggacgta aacacaccgc agaagatgtg
      361 tatcaaacta tgcaattttt agaagcagaa aattttacca atgtaagcat cgatttgatt
      421 tatgcattac cgggacaaac actagaagga tatctggata ctttggaaaa agctttggct
      481 ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac aatgttcatg
      541 aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac cagaa
//
LOCUS       DANKHJ010000368          591 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000368 DANKHJ010000000
VERSION     DANKHJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.381"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..151
                     /locus_tag="PW113_003624"
     CDS             <1..151
                     /locus_tag="PW113_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL2527834.1"
                     /translation="CCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQ
                     IKYIN"
BASE COUNT          215 a           70 c           86 g          220 t
ORIGIN      
        1 ttgttgcaat gttgacaaag gaatctatta ttatcgaaca tatgaagaca gtcaaattac
       61 tgcaattgat atgaataaag aagacttaga tagtcataag ttaattagtt atccaattat
      121 agaaaaacaa caaattaaat atataaatta gttaatgtgt tgtgattgat tatttaatat
      181 agatataata caaaaaggca aagatatttt caaaacattt tttcttgatg tgatatcctt
      241 gtcttttttg taatgttttt aacttattgc ttttgcaaaa tttatttacc tgttttctgg
      301 atcaacgata gtgtatatta tgctatcctt gtttggattt taggacgttt aaagaggcac
      361 ttttttacat aacttaatcc atagaccttt taagcatgct gtgagataat aataaagaag
      421 agtttaaagc gcaccccaaa ccacttcccc ataagtgtat tacgctttaa actcttttat
      481 atttgaattc actaagaagc ataccatatt tttgaaaaaa agtgagaaaa aaggcttata
      541 attaggattt agagtaatta attagatgtt tattttatag gactgatact a
//
LOCUS       DANKHJ010000369          572 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000369 DANKHJ010000000
VERSION     DANKHJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.382"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..505
                     /locus_tag="PW113_003625"
     CDS             <1..505
                     /locus_tag="PW113_003625"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL2527835.1"
                     /translation="PNFIVLTCNTAHYFFEELQAATDIPILHMPREAANELVRQHTTG
                     RVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINYLIYHEIKESDYLNQELYY
                     EILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQSILADRTIERALAERSEA
                     LDTASEK"
BASE COUNT          201 a           99 c          124 g          148 t
ORIGIN      
        1 accaaacttc attgtgttga cttgtaacac cgcacactac ttttttgaag agttacaagc
       61 ggcaacggat attcctatct tgcatatgcc aagagaagca gcaaatgaac ttgtccgtca
      121 gcatacgaca gggagagtag cgattctagg aacagaagga agtatgaaag ctggaatcta
      181 tgaacgcgaa gtcaaaaatc ttggtttcga aacagtgatt cctgatactg cgcttcaaga
      241 aaaaatcaat tacctgatct atcatgagat caaggaatcg gattatttga atcaagagct
      301 ctattacgaa atcttagagg aagcggtaga gcgcctaaac tgtgaaaaag tcatcctagg
      361 ttgcacagaa ttatctttga tgcatgaatt tgctgaagat aatcattacc cagtgatcga
      421 tgctcaatct attttagcgg atcgaacgat cgaacgtgca ttagctgaaa gaagcgaggc
      481 attggataca gcatcggaaa agtaaaaaaa ttggatttgg ggcaaatatc ctaaaagata
      541 tttggtccaa atctttttgt aaaagaaaaa ga
//
LOCUS       DANKHJ010000370          558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000370 DANKHJ010000000
VERSION     DANKHJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.384"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            59..>558
                     /locus_tag="PW113_003626"
     CDS             59..>558
                     /locus_tag="PW113_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface adhesion protein"
                     /protein_id="HDL2527836.1"
                     /translation="MVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPT"
BASE COUNT          176 a          111 c          164 g          107 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaac
//
LOCUS       DANKHJ010000371          555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000371 DANKHJ010000000
VERSION     DANKHJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.385"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            73..>555
                     /locus_tag="PW113_003627"
     CDS             73..>555
                     /locus_tag="PW113_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2527837.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          208 a          102 c           90 g          155 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      481 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      541 tatgtcgcta ttttt
//
LOCUS       DANKHJ010000372          554 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000372 DANKHJ010000000
VERSION     DANKHJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.386"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>554
                     /gene="polC"
                     /locus_tag="PW113_003628"
     CDS             <1..>554
                     /gene="polC"
                     /locus_tag="PW113_003628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2527838.1"
                     /translation="VLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGP
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSIS"
BASE COUNT          208 a          103 c          123 g          120 t
ORIGIN      
        1 gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat gaagcaagca
       61 gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct
      121 cttgaaggac ccatccagct aggacgaaat attccagccg atgaaccaac gacaccaatg
      181 atcagtatcg ttgaagaaga acgaagagtc acacttgagg ggtacgtatt cgacaaagaa
      241 gtcagagaac tacgttcaaa acgcaagatc ttgactttga aaatcacaga ttatacttca
      301 tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc
      361 agtgtcggta gctggttaaa ggtacgagga agtattcaag aagatacgtt tgtccgtgat
      421 ttggtcatga atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa agattatgct
      481 cctgaagggg aaaaacgagt agagcttcat gttcatagca atatgagtac gatggatgca
      541 acaaacagca tctc
//
LOCUS       DANKHJ010000373          539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000373 DANKHJ010000000
VERSION     DANKHJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.387"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..517)
                     /locus_tag="PW113_003629"
     CDS             complement(<1..517)
                     /locus_tag="PW113_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2527839.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKV"
BASE COUNT          189 a          101 c           73 g          176 t
ORIGIN      
        1 atactttttg atcaatgtta cccatttgat agtattctgg gatatccatt aagctcctaa
       61 actcttcaat acgaactaca taataaccag tagaacggta ctgcataagc agtcggaata
      121 gagtttttgc atacgtagaa cgcaacgacg tgaaagcaga taattcaaat ctactaaatt
      181 cagctgttaa accattaata atatattcta ggtccggatt aacactgact tcaacaaact
      241 tttttgtttt attgatttca aaaccagtaa acaaaacaaa gtagcgaata gaatcctcat
      301 cttcagtacg gtaagtaaga ttaagcatat ctttatagag cttttctaaa tcaccgacaa
      361 aggcttttgt agcagtcatc ttataatcac ttaactcttt taattcctcg aaagaaaatc
      421 taactttttt caaaccacga tctttcatac gagcgcataa tgcaaaaaat aaatccatct
      481 gcttagcatt gaaatttttc agcggtacta aattcaattc attttgatat ttgactgtt
//
LOCUS       DANKHJ010000374          539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000374 DANKHJ010000000
VERSION     DANKHJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.388"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>539
                     /locus_tag="PW113_003630"
     CDS             <1..>539
                     /locus_tag="PW113_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2527840.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPM"
BASE COUNT          156 a          113 c           96 g          174 t
ORIGIN      
        1 ttcctattct ttatttaatg gaacaacctg ctttgtctgt ttctcaatca tttttagaca
       61 aaaatgaaat ttcttatatg acccatactt ctggtacaac tggtatccct aaacttattt
      121 gtcattctgc tcattctatg ggatggcgga cgaaatggca aaaaacagtt ttcacaaaaa
      181 tttcggaaaa gaaattaatc gcttttcaca tctctccagt gcattccaga ttcaatattg
      241 gtatctcttc tttgatgtcc atgggatttc ctatgatgcc tttagcaaac gcacaaagtt
      301 caaaagttgt tcatatgttg gaagctcacc ggccaattgc actagagacc catccgaata
      361 actttgttca gtggcgtttt actgctaagg agcatccaga agctttttcg ggtatccgat
      421 actatcactc cacttttgat gctataaaca accagacaat gaagacattt ttgcgaacgt
      481 cgatggcaca ggatgcggta tttcttcaag tatatggcca aagtgaatgc ggaccaatg
//
LOCUS       DANKHJ010000375          536 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000375 DANKHJ010000000
VERSION     DANKHJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.389"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          185 a           79 c          113 g          159 t
ORIGIN      
        1 acctttgaaa aagaacatcc aacgcttttc agtgaacaat gatttcttgc ttgaacaccg
       61 tgaattagag gtcgtgaaaa agctgtcctg tatcaagtaa taagaacagc caaacaaaaa
      121 tagctcttca tattttgtcg ttttttgagc taataccgag ggtcagttga ttgaacaccg
      181 tttatatcag aggttgtgag cctgccgttt aactctgagt cacaaggagc aatttcaaaa
      241 aacagcttct catgtttgat gaatattttg acttgtgacc gaagagttgg taggagaaca
      301 ccgtttataa gaggttgtga caaaagtctt gtcgcaacct cttttttaac caaaaaagtg
      361 caaatagttt actttaagta agagaataat tatgatgaat aaaaaaggtg gaacaaatag
      421 catactatat atattgaaat gaacactgta tggatgctcc aacttgatga agctaaggag
      481 gaattcgtat gtcagaaaag gaattaatga aggtagtcgg tttttatgaa gggttg
//
LOCUS       DANKHJ010000376          534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000376 DANKHJ010000000
VERSION     DANKHJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.390"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>534
                     /locus_tag="PW113_003631"
     CDS             <1..>534
                     /locus_tag="PW113_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL2527841.1"
                     /translation="ARSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVAD
                     DIYGRLVYNGNEFTPIATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMI
                     AVASQSTSNPTAVSQYAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKP
                     QGAFYLFPNVKETLRMC"
BASE COUNT          172 a          102 c          120 g          140 t
ORIGIN      
        1 agcacgttca gaaaaaacaa aagccgtaat catcaattct ccatcgaatc cgactggaat
       61 gatttatagc aagaatgaac tgcaggcaat cggtgaatgg gcagtaaaaa atgatatttt
      121 gatagttgct gatgatattt atggccgttt agtttataat ggcaatgagt ttactccaat
      181 tgcaacgatt tcagaagcaa tcaaaaatca gaccatcatc atcaacggcg tgtctaaaac
      241 atatgcgatg acaggctggc gcattggtta tgcggtggga aatcctgtga tcatcaatgg
      301 aatgatcgct gttgcttccc aatcaactag caatccgaca gcagtcagtc aatatgcagc
      361 tgttgaagca ttgaaaggtg agcaagatac agtagaagaa atgcggaacg cctttgaaga
      421 acgcctgaat acgttatatc cgcttgtggc agaactgccg ggtgtttctc ttgaaaagcc
      481 gcaaggagct ttctatcttt ttcctaatgt aaaagaaacg ctacgcatgt gtaa
//
LOCUS       DANKHJ010000377          534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000377 DANKHJ010000000
VERSION     DANKHJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.391"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>534
                     /locus_tag="PW113_003632"
     CDS             <1..>534
                     /locus_tag="PW113_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2527842.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DANKHJ010000378          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000378 DANKHJ010000000
VERSION     DANKHJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.392"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(5..90)
                     /locus_tag="PW113_003633"
     tRNA            complement(5..90)
                     /locus_tag="PW113_003633"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="PW113_003634"
     tRNA            complement(107..178)
                     /locus_tag="PW113_003634"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="PW113_003635"
     tRNA            complement(190..262)
                     /locus_tag="PW113_003635"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="PW113_003636"
     tRNA            complement(280..361)
                     /locus_tag="PW113_003636"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="PW113_003637"
     tRNA            complement(369..441)
                     /locus_tag="PW113_003637"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="PW113_003638"
     tRNA            complement(445..517)
                     /locus_tag="PW113_003638"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DANKHJ010000379          516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000379 DANKHJ010000000
VERSION     DANKHJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.394"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>516
                     /locus_tag="PW113_003639"
     CDS             <1..>516
                     /locus_tag="PW113_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL2527843.1"
                     /translation="PLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFVDYFLSLKNPIA
                     NQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTATTNSDNKYAAQA
                     SKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMKIIDGKNAQVIV
                     GTEVQFVADEMA"
BASE COUNT          156 a          109 c          109 g          142 t
ORIGIN      
        1 ccattatatg tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc
       61 cattggacag caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa
      121 aatccgattg cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt
      181 tactactttg gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag
      241 gaagcagatc tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca
      301 caagcaagca aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac
      361 tgtactacac gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc
      421 aaagccactg gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta
      481 ggtacagaag tacaattcgt tgcagatgag atggca
//
LOCUS       DANKHJ010000380          510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000380 DANKHJ010000000
VERSION     DANKHJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.395"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(109..>510)
                     /locus_tag="PW113_003640"
     CDS             complement(109..>510)
                     /locus_tag="PW113_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2527844.1"
                     /translation="GDLYVVFRVEESDIFDREGSEIYYELPLSFVQAALGDEVQVPTV
                     HGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGDQHVKVKLITPKNLNEQQREALRA
                     FAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT          136 a          118 c           85 g          171 t
ORIGIN      
        1 ccaggcgaac gtatgattgt tcgtctggtt tttaattatt aatgacttag cgtttttact
       61 agaaatagaa aatttgggca agtctacttt atatttttac tagaaaaact acttccgctt
      121 tttcccgcca aaagcatctt taaacttatc gaagaaaccg tcttcttgct gttcttcaac
      181 tttgatgccg ccagcttcag cgaatgcacg taatgcttct ctttgttgtt catttagatt
      241 tttcggtgtg atcagtttta ctttgacatg ttggtcgcct gttgcaccac cacgtaactt
      301 cggagcgcct tttccacgta aacggaagtt cgtacctgtc tgtgttccag cagggatctt
      361 caatttcaca tctccatgga cagttggtac ttggacttca tcccccaaag ctgcttgtac
      421 aaaacttaaa ggcaattcat agtaaatttc agaaccttca cgatcaaaga tatcactttc
      481 ttctacacga aaaaccacat acaaatcacc
//
LOCUS       DANKHJ010000381          506 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000381 DANKHJ010000000
VERSION     DANKHJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.396"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..380
                     /locus_tag="PW113_003641"
     CDS             <1..380
                     /locus_tag="PW113_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2527845.1"
                     /translation="KFNHPLVFESDSSENHYNGKHYHQHHQEHEFKSILLTSDQDIKK
                     DFLFQLIDRLILTNQGSLYRFKGLIALQDQDFIVAIQGVNEQVNFQYTTRQKDQTTII
                     LIGKMLDEERIKQSFDKLLAESK"
     gene            410..>506
                     /locus_tag="PW113_003642"
     CDS             410..>506
                     /locus_tag="PW113_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2527846.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHK"
BASE COUNT          206 a           79 c           81 g          140 t
ORIGIN      
        1 aaaaattcaa tcatccctta gtttttgaaa gtgattcatc agaaaatcac tataatggca
       61 aacactatca tcaacatcat caagaacatg agtttaaatc aattctctta acttcagatc
      121 aagacataaa aaaagatttt ttattccaat taattgaccg gctgatttta accaaccaag
      181 ggagcctgta tcgttttaaa ggattgattg ctttgcaaga tcaagatttt atagttgcca
      241 tacaaggggt aaacgaacag gttaattttc aatacaccac tcgacaaaaa gaccaaacca
      301 ctattattct aattggaaaa atgttggatg aagaaaggat caaacagagt tttgataaat
      361 tactggcgga aagcaaataa tgataagtat tgattagggg taatttctaa tggagaaaac
      421 ggatatatct agtgcatatc gacgacttaa aagcccgaat atcaaaacta gaaaaagggc
      481 attaaaaatt atcaaagaac ataaac
//
LOCUS       DANKHJ010000382          496 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000382 DANKHJ010000000
VERSION     DANKHJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.398"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          166 a           91 c          102 g          137 t
ORIGIN      
        1 acgaatatca tcacaccaat cgtcttgatc ttattaggta tgatcttgcc agcaattgct
       61 cgtcgagaca aaaacaaaga aatcactgaa ccagaaacag tggttcagcc ttaaaataga
      121 aatacttgtt acaagaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt
      181 acgaaaattg cttctcaaat ttttgtgaag tttcggcttg tttttcgaag aagctacttc
      241 tggaacaccg tgaatcagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc
      301 caaacaaaaa tagcccctca tatttttcgt ttggctgggc ttattaccgc agatgcaagc
      361 tttggaacac cgtttaagga agaggttgta aaatcagcga tttgacctga gtattagaga
      421 aacaaacgga aaaatagctc ctcatatttt gccgcttttt gagctaatgc tgaaggtcag
      481 ctgattgaac accatt
//
LOCUS       DANKHJ010000383          493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000383 DANKHJ010000000
VERSION     DANKHJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.400"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..300
                     /locus_tag="PW113_003643"
     CDS             <1..300
                     /locus_tag="PW113_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527847.1"
                     /translation="EEGKYEQVQFKSAEFLPGTVVEDAAGVMEEMQTATDKQSVKKAL
                     SRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAGFDETYTETKKK"
BASE COUNT          170 a           66 c          111 g          146 t
ORIGIN      
        1 gaagaaggta aatatgagca agtacaattt aaatctgctg aatttttacc aggaacagtt
       61 gtagaagatg ctgcaggagt tatggaagaa atgcaaacag cgactgataa gcaatcagtt
      121 aaaaaagctt taagtcgtgc ttattcattt attgcagata ctttatttga gggacaattt
      181 actggtgaag attattgcaa aggaattgat gctcgtgaga ttgcctcatt gacaggaaaa
      241 ttattgaagt ctgttactgc aggttttgat gaaacttata cagagacgaa aaaaaagtaa
      301 gtgaggcgct caagtcacct tcatttaagt attcgattac ttaccgagaa ttagatataa
      361 agacgcaatt actcgaagca ggttggacgt taccagaaat tgaacgtact gacttggatg
      421 agttaatgcg tctttttgct tttagagatg cggtaaaaga gcatgaagat gttgagtact
      481 atgataattt cac
//
LOCUS       DANKHJ010000384          488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000384 DANKHJ010000000
VERSION     DANKHJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.401"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..132)
                     /locus_tag="PW113_003644"
     CDS             complement(<1..132)
                     /locus_tag="PW113_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2527848.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          173 a           91 c           90 g          134 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaa
//
LOCUS       DANKHJ010000385          487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000385 DANKHJ010000000
VERSION     DANKHJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.402"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>487)
                     /locus_tag="PW113_003645"
     CDS             complement(<1..>487)
                     /locus_tag="PW113_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL2527849.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DANKHJ010000386          487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000386 DANKHJ010000000
VERSION     DANKHJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.403"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>487)
                     /locus_tag="PW113_003646"
     CDS             complement(<1..>487)
                     /locus_tag="PW113_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2527850.1"
                     /translation="SFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLNYTVY
                     EVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLLFIVD
                     AMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSLSLDL
                     Y"
BASE COUNT          146 a          110 c           88 g          143 t
ORIGIN      
        1 tcatatagat caagtgacaa acttcggcta acattttttc gtttttccag ttccgcttta
       61 ttagcaagta caaaagaaaa gcctggaaca ccttgaatac atttgtttga gctggaaatc
      121 aaaaaatcaa tccctagttc ggctacacaa atcggtatcc caccaaagct tgacatcgcg
      181 tccactataa acaaaagatc tctttcttgt gtgacagcag caatctcttc caaattattc
      241 aaaataccgg aagtagtctc gctatggacc attgctagtg cggagataga cggattctct
      301 tcaatcagct gtgtaatcac atgagcttgg aggatttgat gttccgatac ctcataaacg
      361 gtatagttca gtttcagaca ttcgcacatg ttgatcattc gttgtctata tgcgccgttt
      421 gaacaaatca gtacagtatc ttctggcctg atcacagaac ttaataccga ttctaccccg
      481 aaggagc
//
LOCUS       DANKHJ010000387          482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000387 DANKHJ010000000
VERSION     DANKHJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.406"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..294
                     /gene="truB"
                     /locus_tag="PW113_003647"
     CDS             <1..294
                     /gene="truB"
                     /locus_tag="PW113_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2527851.1"
                     /translation="DALTLEEVAQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKV
                     KNGMRLDYTVFALPEMPSEEIALFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            298..>482
                     /locus_tag="PW113_003648"
     CDS             298..>482
                     /locus_tag="PW113_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL2527852.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPS"
BASE COUNT          185 a           74 c          104 g          119 t
ORIGIN      
        1 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
       61 ttgctgccaa tagaaaaagg agtggaacgg ttcaaacgag tagatattac tgatgaagta
      121 tggcagaaag taaaaaacgg tatgagactt gattatactg tatttgcgct cccagaaatg
      181 ccgtcagaag aaattgcttt gttttataaa ggaaaagttg tcagtattta tcagccaaac
      241 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
      301 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
      361 ttagtgctgg gatttttcga cggcgtgcat atcggacacc agaaagtcat aaagacaggt
      421 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
      481 tc
//
LOCUS       DANKHJ010000388          481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000388 DANKHJ010000000
VERSION     DANKHJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.407"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..209)
                     /locus_tag="PW113_003649"
     CDS             complement(<1..209)
                     /locus_tag="PW113_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2527853.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..>481)
                     /locus_tag="PW113_003650"
     CDS             complement(308..>481)
                     /locus_tag="PW113_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527854.1"
                     /translation="AEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFRE
                     FRNKIIGFSQTTK"
BASE COUNT          146 a           89 c           58 g          188 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 c
//
LOCUS       DANKHJ010000389          476 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000389 DANKHJ010000000
VERSION     DANKHJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.408"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>476
                     /locus_tag="PW113_003651"
     CDS             <1..>476
                     /locus_tag="PW113_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2527855.1"
                     /translation="DDGAGSQVETGNVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTER
                     QAGSSIKPVLVYGPAIDMGLIGSESRVSDYATTWQEGENAGEKIVNATNEGSNTFQTV
                     RESLEWSNNIPAYHLYQDVLNSGGSKQYAYEHYLAKMNYPANDNWGVESAPLGTVDV"
BASE COUNT          149 a          101 c          112 g          114 t
ORIGIN      
        1 gatgatggtg ctggcagtca agtagaaacc ggaaatgtat tgatggataa cagaactggc
       61 cgcatctacg gattcgttgg cggacggaat tacagtcaga atcaaaataa ccatgccttc
      121 gatacagaac gtcaagctgg ctcatcaatc aaaccagttc ttgtttatgg acctgcaatc
      181 gacatggggc ttatcggatc agaatcccgt gtatcagatt atgcaacaac ttggcaagaa
      241 ggagaaaatg ctggcgagaa aatcgtcaat gctactaacg aaggatcgaa caccttccaa
      301 accgtccgtg agtcactaga atggtccaac aatatcccgg cttatcactt atatcaagat
      361 gtgttgaatt ccggtggatc gaaacaatat gcttacgagc actacttggc aaagatgaac
      421 tacccggcaa atgacaattg gggcgttgaa tctgctcctt taggtacagt agatgt
//
LOCUS       DANKHJ010000390          470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000390 DANKHJ010000000
VERSION     DANKHJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.409"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>470
                     /locus_tag="PW113_003652"
     CDS             <1..>470
                     /locus_tag="PW113_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2527856.1"
                     /translation="TETEINGLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLR
                     KGFYQELIDIIREKGADFVIDTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEE
                     IIPYGKKLLAEGAQNVLISMAGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIA"
BASE COUNT          162 a           83 c          109 g          116 t
ORIGIN      
        1 actgaaactg agatcaatgg cttaggccca tctatttcag atgaagaaat cgatgagttg
       61 aagcaagcat taggaacagt aggacaagga gatatcgtcg tattgtcagg aagcgcgcca
      121 gctagcctgc gaaaaggatt ttatcaagaa ctgattgata tcattcgaga aaaaggtgca
      181 gatttcgtga ttgatacaac aggtgatgac ctaaaaaatg cacttgttaa gaaaccgttg
      241 ttgatcaaac caaacaatca cgaattagct gagctttaca acacaacctt ccaatctgta
      301 gaagaaatca ttccgtatgg gaaaaaatta ctagctgaag gggcacagaa tgtgctgatc
      361 tcaatggctg gagacggtgc tttgctcttc acggaacaag gaacttataa aacgaatgta
      421 ttagttcgcc cattgaaaaa ttcagtaggt gcaggggatt caatgattgc
//
LOCUS       DANKHJ010000391          468 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000391 DANKHJ010000000
VERSION     DANKHJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.410"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>468
                     /locus_tag="PW113_003653"
     CDS             <1..>468
                     /locus_tag="PW113_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2527857.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DANKHJ010000392          467 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000392 DANKHJ010000000
VERSION     DANKHJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.411"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>467
                     /locus_tag="PW113_003654"
     CDS             <1..>467
                     /locus_tag="PW113_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2527858.1"
                     /translation="SDRQSQAILDMRLRRLTGLEREKIENEYQELLKLIEDLTDILAR
                     PERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVITLTNNGYIKR
                     LANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKVYRAKG"
BASE COUNT          164 a           78 c          103 g          122 t
ORIGIN      
        1 tccgatcgcc agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa
       61 cgggaaaaaa tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac
      121 attctggcaa gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat
      181 caacgatttg gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa
      241 gatgaagatt taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc
      301 aaacgtttgg caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga
      361 atgggtatcc acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca
      421 ttgttattct ttacgaataa cggaaaagtg tatcgggcaa aaggata
//
LOCUS       DANKHJ010000393          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000393 DANKHJ010000000
VERSION     DANKHJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.412"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..430)
                     /locus_tag="PW113_003655"
     CDS             complement(<1..430)
                     /locus_tag="PW113_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2527859.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANKHJ010000394          460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000394 DANKHJ010000000
VERSION     DANKHJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.413"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(20..91)
                     /locus_tag="PW113_003656"
     tRNA            complement(20..91)
                     /locus_tag="PW113_003656"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="PW113_003657"
     tRNA            complement(113..200)
                     /locus_tag="PW113_003657"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="PW113_003658"
     tRNA            complement(225..298)
                     /locus_tag="PW113_003658"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="PW113_003659"
     tRNA            complement(312..382)
                     /locus_tag="PW113_003659"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="PW113_003660"
     tRNA            complement(384..459)
                     /locus_tag="PW113_003660"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DANKHJ010000395          414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000395 DANKHJ010000000
VERSION     DANKHJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.420"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..362
                     /locus_tag="PW113_003661"
     CDS             <1..362
                     /locus_tag="PW113_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2527860.1"
                     /translation="DGRVIVVLGSPGNKAISRRHDFGKVLSETADIAFLTADDPAFED
                     PKKIAEEINEAITNPDLIVHYEMDRPEAIRRALAESAPQDSVVIAGKGVDPYQKINGV
                     DEPYEGDYAIVKRLIEE"
BASE COUNT          135 a           74 c           99 g          106 t
ORIGIN      
        1 cagatggccg tgtaattgtt gtattaggca gcccaggaaa caaagcaatc tcccgtcgac
       61 atgattttgg aaaagtgcta tcagaaacag ctgacatcgc ctttttgacg gcagatgacc
      121 cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa tgaagcaatt actaatccag
      181 atttgatcgt tcattatgaa atggatcgtc ctgaagcgat tcgacgcgct ttagcagaaa
      241 gcgctccgca agattcagtc gttattgcgg gaaaaggcgt ggatccctat caaaaaatca
      301 atggtgtaga tgaaccttat gaaggggatt atgcaatcgt gaagcggttg attgaagaat
      361 aatggtattt atatactagt ggtttcccat ttctaagaaa aaaagggact gtga
//
LOCUS       DANKHJ010000396          414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000396 DANKHJ010000000
VERSION     DANKHJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.421"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..122)
                     /locus_tag="PW113_003662"
     CDS             complement(<1..122)
                     /locus_tag="PW113_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2527861.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSS"
     gene            complement(133..330)
                     /locus_tag="PW113_003663"
     CDS             complement(133..330)
                     /locus_tag="PW113_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2527862.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
BASE COUNT          115 a           80 c           63 g          156 t
ORIGIN      
        1 gagctacctt cgtttgggcc taaattaaat tcattgttaa tcgtataggc aaacccatta
       61 attggcaata aaaaaagagc cgttaatagg ctcatcgcag taatagtaat tttctttttc
      121 atttgtttcc tcctattttt tcaaattata agccgacacg ccagtgataa cgcctaaaaa
      181 tgttgctact gcattgatag tgagtactgt catatctgtt ccattccatc catacgcttt
      241 gcctagtgtt gcaactaata cagatgcagc tggcaatact gttaaaactg tccatttaat
      301 gacttgataa tacttatcgg gtaaaatcat ttcttcttaa ctcctttctt ttttaccgag
      361 atctttctct aaataaagtt ttgatttgtt gtgtgtgttc taccaatttt tctg
//
LOCUS       DANKHJ010000397          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000397 DANKHJ010000000
VERSION     DANKHJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.423"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..387
                     /locus_tag="PW113_003664"
     CDS             <1..387
                     /locus_tag="PW113_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527863.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          155 a           73 c           63 g          113 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc agga
//
LOCUS       DANKHJ010000398          399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000398 DANKHJ010000000
VERSION     DANKHJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.424"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..85)
                     /locus_tag="PW113_003665"
     CDS             complement(<1..85)
                     /locus_tag="PW113_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2527864.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNE"
BASE COUNT          154 a           71 c           58 g          116 t
ORIGIN      
        1 tctcattttg gtttagtgtg ctagtaatat aagcaccttc ccaatcggca tattcctcta
       61 atagaaacgt aatcgcagtt ttcattttca agctctcctt tactatctat aacaaggaca
      121 acagttttaa cctacagaca ttcattaaaa ttgggtagcg acagcgaaaa caacaattct
      181 taagttttag acagagtata cagatagtca acttaattaa atacgaacta aataaaaatg
      241 acaggtaatc atttttgttt accagcatta taacattttg catacatgga tgacaaaaga
      301 accaattccc acttgcttga gtacaatatt aaaagaaact ggaaggcaaa aagtaattat
      361 agaaacgaaa aataaatcat atcgggcact cagacatga
//
LOCUS       DANKHJ010000399          385 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000399 DANKHJ010000000
VERSION     DANKHJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.425"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            189..>385
                     /locus_tag="PW113_003666"
     CDS             189..>385
                     /locus_tag="PW113_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2527865.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLD"
BASE COUNT          158 a           63 c           75 g           89 t
ORIGIN      
        1 aaaataatgt attgcaagca caaacaggtt actttggaac agagacagtt tcaattgctc
       61 ttcataacta attagtataa aaaacacgcc ccaccgacca aagcgagcgt gttctaagaa
      121 aaaacaaacc tacacaatag gcttctttat agtacctatt gtatcagaga aagagagctg
      181 attcaattat gtcagaagta acaggatact tagaacaagt agacaatgaa acatatagac
      241 taagagctgt attagggtac aaacctgatg gctcggcaaa aagaaaaagc aaaactataa
      301 aagcgaatag taaaagagcg gcatataaag aattgaacat ctggttagag caattcgaag
      361 gaatgaccga tgattcgttg gattt
//
LOCUS       DANKHJ010000400          384 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000400 DANKHJ010000000
VERSION     DANKHJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.426"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>384
                     /locus_tag="PW113_003667"
     CDS             <1..>384
                     /locus_tag="PW113_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL2527866.1"
                     /translation="FKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEYE
                     VLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRAL
                     GATRKSIMLQFLLEGLILTLSGGLI"
BASE COUNT          129 a           52 c           88 g          115 t
ORIGIN      
        1 attttaagtc tgaaaaggat acatcgtctc taacgttaac tttagaggaa ggagttgaac
       61 ctgacaaggt gacctctaaa gtgataaacc aattgaaaga aaaaggtagt ttgaaacatt
      121 tgggggaata tgaagtgtta gatacggcta tgttaacgaa aggaattggt caaatcctca
      181 gtacgatcac ttattttata acagctgtag caggtatatc tttatttatc gcaggggtag
      241 gtgtaatgaa catgatgtat atatctgtat ctgaacgaac aaaagagatc ggtataagaa
      301 gagccctagg agctactcga aaatcgatta tgctgcagtt ccttttagaa ggtttgattt
      361 taactctttc aggagggtta attg
//
LOCUS       DANKHJ010000401          381 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000401 DANKHJ010000000
VERSION     DANKHJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.427"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(21..94)
                     /locus_tag="PW113_003668"
     tRNA            complement(21..94)
                     /locus_tag="PW113_003668"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="PW113_003669"
     tRNA            complement(108..181)
                     /locus_tag="PW113_003669"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="PW113_003670"
     tRNA            complement(209..281)
                     /locus_tag="PW113_003670"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="PW113_003671"
     tRNA            complement(301..374)
                     /locus_tag="PW113_003671"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DANKHJ010000402          367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000402 DANKHJ010000000
VERSION     DANKHJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.431"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..289
                     /locus_tag="PW113_003672"
     CDS             <1..289
                     /locus_tag="PW113_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527867.1"
                     /translation="NGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQL
                     RLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          106 a           70 c           92 g           99 t
ORIGIN      
        1 gaatggtttt ggaattactc gttcattgag taggaagggt tgtccgtatg ataatgccgt
       61 tgtggaatca acctataaat ctgtcaaagt agaattcgtg catcaatacc aatttgagac
      121 actggcacag ctacgtctag aattgtttga ttatgtgcat tggtggaact atctacgctt
      181 acatggcacg ttggcgtatg aaacaccgat ccaaattcga caacagagat tggcgaagcg
      241 aatccttgat aatgagcgcg gatctgatac ctctggagag gcagcgtaat tgaatgattg
      301 tgcttctgcc ggagaaaatc agatccgagg atgctcattg tcaagggcaa tcgtagccat
      361 aacaccg
//
LOCUS       DANKHJ010000403          361 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000403 DANKHJ010000000
VERSION     DANKHJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.433"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(2..226)
                     /locus_tag="PW113_003673"
     CDS             complement(2..226)
                     /locus_tag="PW113_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2527868.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           94 a           84 c           54 g          129 t
ORIGIN      
        1 ttcatttgtt tcctccttct tcgctttcag ccgagaacat tttgtaggtt cgatttgata
       61 cacccaacac actccctaaa aacgcgccaa aaccagtaat gatgacaaca cagatatctg
      121 tgtactgcca attgagcgct ttaccaacta accccacgaa agtagctagt gcgggaataa
      181 ttaccagtgc gaaccatttt agtacttcga acgttttatt attcattttc ttctctccct
      241 aaacaaagtt ttgatttgtt gcgtgtgttc caccaatttt tctgtatgtg tatctaatct
      301 ttcatcgtgt ttctttagtt cttcatgaat catcaatcga tctgatttgc tcgattctaa
      361 a
//
LOCUS       DANKHJ010000404          351 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000404 DANKHJ010000000
VERSION     DANKHJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.435"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            17..>351
                     /locus_tag="PW113_003674"
     CDS             17..>351
                     /locus_tag="PW113_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2527869.1"
                     /translation="MKNNGFKYTKKREALLTYLVKRNRYVSAKEVFDYMNQEFKGVSY
                     DTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICTVCGTTKEIHMCPM
                     NYFEEQLGG"
BASE COUNT          126 a           55 c           71 g           99 t
ORIGIN      
        1 acaatcacta gaaacattga aaaataacgg ttttaaatat actaaaaaaa gagaagcatt
       61 gttaacttat ttagtgaagc gcaatcgcta cgtatccgct aaagaagttt ttgactatat
      121 gaatcaggaa ttcaaaggag taagttacga tacgatttat cgaaatctcc atgattttgc
      181 agaaatcgat ttactggaag aaaccgattt aaatggagag atgaagtttc gttttcattg
      241 ctgtcaaggg gaaatcggcc atcaccatca ctttatctgt acggtgtgcg gaacaacgaa
      301 agaaatccat atgtgtccaa tgaattattt cgaagaacag ctcggtggat g
//
LOCUS       DANKHJ010000405          346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000405 DANKHJ010000000
VERSION     DANKHJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.436"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(71..>346)
                     /locus_tag="PW113_003675"
     CDS             complement(71..>346)
                     /locus_tag="PW113_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2527870.1"
                     /translation="ETYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYD
                     ERMASRVRKAFFEDSKISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          100 a           74 c           58 g          114 t
ORIGIN      
        1 cagaaatggc cccgttagaa aaggacacga gatctccatc tgttgatggt tctcatgtcc
       61 tttttatgtt ttaaaggagc ggcgaaatca atcttgctaa tccttctttc atcttgatga
      121 ttattcctcg gttttcatac atttcttttg tgagatgcgt agaaattttc gagtcttcaa
      181 aaaaagcttt tcttacacgt gaagccattc gttcatcata aaccagtgta ttgacctcaa
      241 agtccaagcg aaatgaacga acatcgatat ttgctgagcc aactgaaaca atgccaccat
      301 cgataatcat cgtctttgca tgaataaatc cattctcata tgtttc
//
LOCUS       DANKHJ010000406          346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000406 DANKHJ010000000
VERSION     DANKHJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.437"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            4..219
                     /locus_tag="PW113_003676"
     CDS             4..219
                     /locus_tag="PW113_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527871.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWQTTQPRLRDSLIFLEIQLE
                     NLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            222..>346
                     /locus_tag="PW113_003677"
     CDS             222..>346
                     /locus_tag="PW113_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HDL2527872.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIG"
BASE COUNT          108 a           63 c           70 g          105 t
ORIGIN      
        1 ctgatgtcaa cgataacaac gattaccatg gtggccagtc cacttggatt agcactgttt
       61 ggtgcgctct ttgattggca gacaactcaa ccacgtctgc gagacagtct gatttttctt
      121 gaaattcaat tggaaaattt acactttctt tatacatttc tggctgcttt ctttataccc
      181 gtcaactata cttcacttat caataagaaa gaaggataag tatggatttt ggaaatgaaa
      241 cggtcttgaa agcaactggt ataaccaaaa aatacggtgc tgccaaagca ttagataaag
      301 tatcgatcga aatcaaacgt ggaatgatct atggtttgat tgggga
//
LOCUS       DANKHJ010000407          341 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000407 DANKHJ010000000
VERSION     DANKHJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.439"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(45..>341)
                     /locus_tag="PW113_003678"
     CDS             complement(45..>341)
                     /locus_tag="PW113_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2527873.1"
                     /translation="PIITDMPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLIS
                     RQILYYSFCVPDSFSNYRISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT           93 a           88 c           55 g          105 t
ORIGIN      
        1 taaatagcta aaaaccgcca tcattccgcc aaaaaaccgc caaattattt ataagcaatt
       61 attcttccgt gtttatatgc ttctgcaaac tctattagag cttccgactt catccgttgt
      121 atacttcttt ctgaataacc cacttcacgg ctaattctgt agtttgagaa gctatctggc
      181 acacaaaagc tgtagtagag tatctgacgg ctaatcagac taagagccat caaagccgct
      241 aaaatcgcat ctctctctgc ttctatatcc atcatctgaa tgatcgcgtc ctctgcctta
      301 ttgccgtgct tcggcgcctt cggcatatcc gtaataattg g
//
LOCUS       DANKHJ010000408          336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000408 DANKHJ010000000
VERSION     DANKHJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.441"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..>336)
                     /locus_tag="PW113_003679"
     CDS             complement(<1..>336)
                     /locus_tag="PW113_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2527874.1"
                     /translation="KKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQAIN
                     FAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIK
                     YIYTVKKNT"
BASE COUNT          112 a           40 c           58 g          126 t
ORIGIN      
        1 tttgtatttt ttttgacggt ataaatatat ttaatcgtat tatcagcgtc tgaaaatact
       61 ggaaagaaaa tagaactatt tgccgtattt tctccatcga gcattttaaa tggcgaacca
      121 agagtatagt taatatcagt ttcggtgtta ttttccatta aaagaattga agttatggag
      181 tgttcaaaat cagttttcgc aaaattaatt gcttgttctt caattggttg ggagttgata
      241 ttgaaattag aattttctga agcataggta tttgtaattc ctatactaaa aattccaatc
      301 atcaaggaaa agaggagacc tagataaatt ttcttt
//
LOCUS       DANKHJ010000409          333 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000409 DANKHJ010000000
VERSION     DANKHJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.442"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          124 a           54 c           59 g           96 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaa
//
LOCUS       DANKHJ010000410          330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000410 DANKHJ010000000
VERSION     DANKHJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.444"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          102 a           70 c           53 g          105 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta
//
LOCUS       DANKHJ010000411          329 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000411 DANKHJ010000000
VERSION     DANKHJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.445"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(60..>329)
                     /locus_tag="PW113_003680"
     CDS             complement(60..>329)
                     /locus_tag="PW113_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527875.1"
                     /translation="KFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIP
                     ATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          104 a           64 c           49 g          112 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattt
//
LOCUS       DANKHJ010000412          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000412 DANKHJ010000000
VERSION     DANKHJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.448"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(69..321)
                     /locus_tag="PW113_003681"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="PW113_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DANKHJ010000413          309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000413 DANKHJ010000000
VERSION     DANKHJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.452"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            1..309
                     /locus_tag="PW113_003682"
                     /pseudo
     CDS             1..309
                     /locus_tag="PW113_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DANKHJ010000414          309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000414 DANKHJ010000000
VERSION     DANKHJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.454"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PW113_003683"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PW113_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2527876.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DANKHJ010000415          304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000415 DANKHJ010000000
VERSION     DANKHJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.457"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
BASE COUNT          103 a           55 c           54 g           92 t
ORIGIN      
        1 atcgtttaaa acagtctgtt gctcttagat tccaacaagc cggcgctgat atttgtgtaa
       61 acaatttaaa agagttaaca gaatacattt tgtacgctga catgaatcca gaatgacttt
      121 tatacaaaat aagaggttgt gaaaaagctg tcctgtatca agtaataaaa acagccaagc
      181 aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg caagcttttg
      241 aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg acttttgtca cagtcccctt
      301 attt
//
LOCUS       DANKHJ010000416          304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.458, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000416 DANKHJ010000000
VERSION     DANKHJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.458"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>304
                     /locus_tag="PW113_003684"
     CDS             <1..>304
                     /locus_tag="PW113_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2527877.1"
                     /translation="EKGYGKRTKVSEYPVKGRGGKGIKTANITEKNGPLAGLTTVSGD
                     EDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEADTKVVTMAAVEPEAAEQTV"
BASE COUNT          106 a           56 c           77 g           65 t
ORIGIN      
        1 cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag
       61 gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca
      121 ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca
      181 acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg
      241 aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag
      301 ttga
//
LOCUS       DANKHJ010000417          302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.459, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000417 DANKHJ010000000
VERSION     DANKHJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.459"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..>302
                     /locus_tag="PW113_003685"
     CDS             <1..>302
                     /locus_tag="PW113_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2527878.1"
                     /translation="GYPSLTITNVAGSTLSREASFTLLLHAGPEIAVASTKAYTAQIA
                     VLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYLEELAEVYLSDTR"
BASE COUNT           95 a           61 c           58 g           88 t
ORIGIN      
        1 ggctaccctt cacttacgat tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc
       61 tttacactgc tattgcatgc tggacccgaa atcgcagtag catcaacgaa agcttatact
      121 gcacaaatag ctgtactaac tttattagcc aaagctatcg gtgataaaaa agaatttgaa
      181 acttctcttg tattcgatat cactcatgaa ttgagtttag ttgctaatgc gatggaatcg
      241 gtcatcgccc aaaaagatta tttagaagag ttggcagaag tatatctcag cgatacaaga
      301 aa
//
LOCUS       DANKHJ010000418          298 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000418 DANKHJ010000000
VERSION     DANKHJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.denovo.461"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..298
                     /locus_tag="PW113_003686"
     rRNA            <1..298
                     /locus_tag="PW113_003686"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           81 a           54 c           91 g           72 t
ORIGIN      
        1 agacagttcg gtccctatcc gtcgcgggcg ttggaaattt gagaggagct gtccttagta
       61 cgagaggacc gggatggact taccgctggt gtaccagttg ttctgccaag ggcattgctg
      121 ggtagctatg tagggaaggg ataaacgctg aaagcatcta agtgtgaagc ccacctcaag
      181 atgagatttc ccatttcttt aagaaagtaa gatccctgag agatgatcag gtagataggt
      241 caggagtgga agtacagtga tgtatggagc ggactgatac taatcgatcg aggactta
//
LOCUS       DANKHJ010000419        29400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.029, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000419 DANKHJ010000000
VERSION     DANKHJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..215
                     /locus_tag="PW113_003687"
     CDS             <1..215
                     /locus_tag="PW113_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527879.1"
                     /translation="YDFSSSLDLFELEAEIIENSREENGKIIISVEKFNELFSETFEI
                     DTDYYQPQEVFQSFRNQIIRSGKTVN"
     gene            complement(268..1041)
                     /locus_tag="PW113_003688"
     CDS             complement(268..1041)
                     /locus_tag="PW113_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527880.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1146..1391
                     /locus_tag="PW113_003689"
     CDS             1146..1391
                     /locus_tag="PW113_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527881.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1547..2398
                     /locus_tag="PW113_003690"
     CDS             1547..2398
                     /locus_tag="PW113_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2527882.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2431..3105
                     /locus_tag="PW113_003691"
                     /pseudo
     CDS             2431..3105
                     /locus_tag="PW113_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            3362..3550
                     /locus_tag="PW113_003692"
                     /pseudo
     CDS             3362..3550
                     /locus_tag="PW113_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3596..4561
                     /locus_tag="PW113_003693"
     CDS             3596..4561
                     /locus_tag="PW113_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527883.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4583..4795
                     /locus_tag="PW113_003694"
     CDS             4583..4795
                     /locus_tag="PW113_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2527884.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4870..5346
                     /locus_tag="PW113_003695"
     CDS             4870..5346
                     /locus_tag="PW113_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL2527885.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5343..7151
                     /locus_tag="PW113_003696"
     CDS             5343..7151
                     /locus_tag="PW113_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2527886.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7228..7443
                     /locus_tag="PW113_003697"
     CDS             7228..7443
                     /locus_tag="PW113_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL2527887.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7535..8398
                     /locus_tag="PW113_003698"
     CDS             7535..8398
                     /locus_tag="PW113_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527888.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8435..8971
                     /locus_tag="PW113_003699"
     CDS             8435..8971
                     /locus_tag="PW113_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL2527889.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8985..9407
                     /locus_tag="PW113_003700"
     CDS             8985..9407
                     /locus_tag="PW113_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2527890.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9337..11736
                     /locus_tag="PW113_003701"
     CDS             9337..11736
                     /locus_tag="PW113_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL2527891.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11768..13759
                     /locus_tag="PW113_003702"
     CDS             11768..13759
                     /locus_tag="PW113_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2527892.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13782..14033
                     /locus_tag="PW113_003703"
     CDS             13782..14033
                     /locus_tag="PW113_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL2527893.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            14023..15252
                     /locus_tag="PW113_003704"
     CDS             14023..15252
                     /locus_tag="PW113_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL2527894.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15249..17330
                     /locus_tag="PW113_003705"
     CDS             15249..17330
                     /locus_tag="PW113_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL2527895.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17479..21399
                     /locus_tag="PW113_003706"
     CDS             17479..21399
                     /locus_tag="PW113_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL2527896.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21400..22344
                     /locus_tag="PW113_003707"
     CDS             21400..22344
                     /locus_tag="PW113_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527897.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22348..22551
                     /locus_tag="PW113_003708"
     CDS             22348..22551
                     /locus_tag="PW113_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL2527898.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22851..23087)
                     /locus_tag="PW113_003709"
     CDS             complement(22851..23087)
                     /locus_tag="PW113_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527899.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23270..23656)
                     /locus_tag="PW113_003710"
     CDS             complement(23270..23656)
                     /locus_tag="PW113_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL2527900.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(23638..24966)
                     /locus_tag="PW113_003711"
     CDS             complement(23638..24966)
                     /locus_tag="PW113_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2527901.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24927..25256)
                     /gene="mobC"
                     /locus_tag="PW113_003712"
     CDS             complement(24927..25256)
                     /gene="mobC"
                     /locus_tag="PW113_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2527902.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25514..25884)
                     /locus_tag="PW113_003713"
                     /pseudo
     CDS             complement(25514..25884)
                     /locus_tag="PW113_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            26029..26214
                     /locus_tag="PW113_003714"
     CDS             26029..26214
                     /locus_tag="PW113_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2527903.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26372..26539
                     /locus_tag="PW113_003715"
     CDS             26372..26539
                     /locus_tag="PW113_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527904.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            27157..27816
                     /gene="vanR"
                     /locus_tag="PW113_003716"
     CDS             27157..27816
                     /gene="vanR"
                     /locus_tag="PW113_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL2527905.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27819..29180
                     /gene="vanS"
                     /locus_tag="PW113_003717"
     CDS             27819..29180
                     /gene="vanS"
                     /locus_tag="PW113_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2527906.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(29271..>29400)
                     /locus_tag="PW113_003718"
     CDS             complement(29271..>29400)
                     /locus_tag="PW113_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2527907.1"
                     /translation="IENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         7628 a         7409 c         7720 g         6643 t
ORIGIN      
        1 tttatgattt tagttcgtca ctagatttat ttgaattaga ggcagaaata atagaaaaca
       61 gtcgagagga gaatggtaaa atcatcattt cagtagaaaa attcaatgaa ttattctcag
      121 aaacatttga aatagataca gattattatc aaccacaaga agttttccaa tcatttagaa
      181 atcagattat tcgctcaggt aagaccgtaa actgaaaaac aataaaagaa acgttgaaag
      241 aataattatt ccttcaacgt ttctttttta gaatgtttta atgaccaaag cagtttcatt
      301 gtcaggctta ggtaattcat gaaatcccgt atttatagtg ctcaattgat caactaacca
      361 tgttttactt tttgaattgt agataaatct aaaattaata tcgttattat cgtgttgaag
      421 gggcgtctcc aaataatctg ctgtatattc atagtcaacg taacctgcta atacaaaaga
      481 gagtatccct ttatttaatg ttagatttaa tggataatca tacgacttta tttttttcaa
      541 cttcatattg tacatatctt cagccataaa agttgagttc gtgatttcat cttgggtctc
      601 attaaaaaag ttatccgaat aatcgcttat ttttgaaaag tctttattgg caccagcctc
      661 aaaaaattgt tcaacttttt cagtataaag ttgtgctaac gctccttttt caatattaac
      721 tttaatattt tcatgtctat caatttgtgt gctgttcagt aagggaagat cagcttttag
      781 ttttatatcg tcttgatttc tttcaatttc aattggcaaa atcttttcag tagattcagt
      841 ttctgataaa ggaacatcta gttttttatc atgataataa gcttcaaatc catctggaac
      901 cggaaaaaaa tgtattgggt aattcttttc tgaggttgtt gtactagttt ccgtacttgt
      961 agaacttgtg tcagaagtgg tacacccgct tatagttccg atgagtaaag cgcaagttag
     1021 aatgattgca aatttcttca aaaaaattcc tccttaaaat tgaatatgga ctaatactag
     1081 tcccattgta gcataccgaa tctttaagat taataagaaa aaggactaat tttagtccaa
     1141 aaggtgtgct attcttgaaa gaggaaaaaa ttattgataa agaaaatgta atcggtagca
     1201 atattagacg agtccgatta gaaaaaggaa ttggtcaaac cgagctgatt agagacctac
     1261 agcttcgtaa aattgctata actagggaaa cgttggttaa gattgaaggt ggccgccaac
     1321 acattaagtt ggatcaattg aaagccatca aagatattct acaggtgtct tatgaagatt
     1381 tgcttcaatg actacttgta gtcatttcgt ttgaaattat tatgttacac ccttatgtgt
     1441 cacgaagaaa tactggtata ataatgaatg ggatatgtaa cgatagggta tactgttatg
     1501 ttgcctaata aattgtggat caaaatacaa gaatggaggg atatccttgt ataaggcata
     1561 tgaattggat tttacaaatg ctgacttaaa tgcattctcc tcaagctcgc aatcatatac
     1621 acatgtagta aatcaaatag atcacaacca aaaatctatt aacaagagaa tagaaaatct
     1681 atttgaaagt gaaaatgatt tagttgatta ttattctaag gacagcaaaa ttctagatgc
     1741 agacgaaatt attaaagatt ggtttccaac tataaaagca gatattttta tatctcactc
     1801 tcatgctgat gaaaaattag ctatacgttt tgcatcatgg ctatttgaaa attttggtct
     1861 tactgcattt attgattctt cggtttgggg atacagttct gatttattaa aaaaaataga
     1921 ccaaaaatat tgctataagg aacaaacaaa aacatatgat tatgataaaa gaaatgtaac
     1981 aactagtcat gttcatatga tgctttctac agctttaaat aatatgattg atagtacaga
     2041 atgtctgttt tttcttaata cacctaattc aattagtttg agcaatgaaa tcactaatga
     2101 acagaaattt acgtattcgc catggctata tagcgaactg acgacagctt caatagtgga
     2161 aaaaaagaat ccacgattgg aaagtaatcc tcaaatgagc accgaggatg ttagaagcat
     2221 tataaagcac tattcttcgc aagaaaatct gcaaataaaa tattctttaa atttaagtga
     2281 attaacttat ttacgaaaag ctgatttgaa aaaatgggct ttagatgctg tggaatgcac
     2341 taaaggaagt aatacattag atattttata taaaaacacc attttcacta atctctaatc
     2401 aatttactga atcgaaagga accgggtaga atggataaga aagttttaat aaagaggttc
     2461 tctgaagcta ttgaaaaagg aaatgcagca atatttgcag gtgcaggatt atcgatgtca
     2521 caaggatatg taagttggcc ggaactactt aatgatcctg caacagaaat cgggttggat
     2581 tctaaaaaag aaacagactt agttactctg gctcaatatt ataaaaatga aaatggtgga
     2641 tcaagaggaa ttttgaatca gatacttatg gataattttg gtgaagaatt agaaatatct
     2701 gaaaatcatc gaatactagc aagtttacct attgaaactt attggacaac taattatgat
     2761 catctaatag aaaaatcaat ccgagaagct tataaaaacc ctcaggttaa gaaaaattat
     2821 actcagttag caacaactaa tccaaatgtg gatacaattg tttataaaat gcatggtgat
     2881 atagatgatg tcagttcgac agtaataaca agagatgatt atgaaaaata tgatgattcg
     2941 tacgcgttat ttaaagaaac tttaaaagga gatttgttaa caaaaacttt tctcttttta
     3001 ggatttagtt ttacagaccc taatttagaa agaattttat cagatataag atgggttttg
     3061 agagaaaacc aaagacctca ttattgtata atgagaaaaa tattatagaa aaaattttag
     3121 gttatcgctg aaagccccgg aaatacgggc atttcgcagg atatgagaac tcaatttact
     3181 accaatttac tactttcgga ttgagccgca tatatgctga accatactgc taatacccgc
     3241 caccccaaat acggcacatc ataaattgaa tacgacaccg taaacgcggc gttcctcttt
     3301 cccgtcatgg gagaacagga cgccgctttt ttatgcccaa atagagaaag gaggcgacgc
     3361 catgctattt acttccggcc ctgaccgggc gtttgaacgg ctgatgcagg aaaagcccgg
     3421 tttcgatcac tacgacggcg gcggggcgga cgcgccggag gattgcggca cctgccgatt
     3481 ttaccgcccg gattggaaat atcaattctg ccagtttgca gagtgtccct atcagccggg
     3541 caagctgacc gactatgcag cggtcaaatt cacagtgaaa ggagcgaaca acgctatggc
     3601 agttttccgt gtggaaaaga acaaaggcta tacggtgatg tcaaatcacc acctgcggaa
     3661 taagaacctg accttaaagg ccaagggcct gctgtcgcag atgttgtcct tgccggagga
     3721 ttgggactat accctaaaag gactgtccta tatcaaccgg gaaagcatcg acgcgatcag
     3781 gacagcggta tgggagcttg aaaaagctgg atatatccgg cgtgagcagg gccgtgacgc
     3841 aaaaggcaaa atggccgata tggtctatac catctacgaa cagccggtat tggaaaaccc
     3901 ggtattggaa aatccaacat cggataaccc ggtgttggaa aatccgacat cggataatcc
     3961 ggcgtcggaa aatccaacgc aattaaataa agaagtacaa agaactgact tatcaaaaaa
     4021 agaaaaaata attacagatg gacaaagtac ccattccatt cctatcccat ccccgacccc
     4081 tgcccctatg gagggcgagg cagcggctac gccgccggaa cggaaaagga caggaaagga
     4141 tgaggctgta cggatttatc gggaaatcat tttggagaac atcgagtatg cctatctcat
     4201 tcaggacagg agcattgacc gtgaacagct tgacgaaatc gtagacctga tgctggaaac
     4261 cgtctgcacc gcccgaaaga caatccgtgt cgccggggac gactaccctg ccgagctggt
     4321 gaaatccaag tacatgaagc tgaccggcga acatatccgc tttgtccttg actgtctgcg
     4381 ggagaacaca acgaaagtcc gcaatatcaa acaatacctg cgggctatgc tgttcaacgc
     4441 ccccagcacc atttccaact actacacggc ccttgtcgcg cacgacatgg cacagcccga
     4501 ttggggaaag cctaaatccg gcctgccgga ctattcctgc ggcgagggcg aaagcctgta
     4561 acgagaaaag gaggacgacc ctatgaagca gggcgcattg atttttgacg agcgaacaga
     4621 ccgttacgac atccgctttg atctggccga ctattacggc ggcctgcatt gcggggagtg
     4681 tttcgacgtg atgatcggcg gcaaatggag gcccacccgc attgaaatgg ccgaaaactg
     4741 gtatcttgtc ggcatccggg ccgacgacct ttccggcctg cgggtgcgga tttgacctat
     4801 atctacctgc ggcgtctggt ggttccgcct tagacgccgc ttttgacttt ctgacaagga
     4861 gggatgcgat tgcaggaaga agttactcaa aagaccattg ccctgtccat gaaaacgggc
     4921 aagctcacgg ctcaagtgct gcaagcggcg ctgaaaaagt acctgcaaca ccgggccaag
     4981 ggcaaaaaca cgctgcacca cggccagcag agcctaaagc agcttaaaaa gcatggggct
     5041 gatctctcca acattgagat caccgaggcc aacattggag cgttcaagcc atgcgccaag
     5101 aaatacggcg tggattttac cttacgaaaa gataagacca cccagccgcc ccactacgtt
     5161 gtgattttca aggccaagga tgcagataac cttgatctgg cgtttcggga gtttaccgcc
     5221 aagacgcttt ccaaggagca gcggccctct atccgcaagg ttttgtccgc tgtgaagcaa
     5281 aaggcggcgg cccagaccaa gcagcgggcc aaggagaaaa tcaaggaaag ggggctggaa
     5341 ctgtgaagcc tgacgtaaag aaattgctga tcctgaacct cccgtatctg atttttgtgt
     5401 acctgtttgc gaaatgcggg gaggcgtacc ggctggccgc tggtgctgac ctgtcgggga
     5461 agctcctgca ttttgcggat ggttttgcgg cggcttttgc caaccctctg ccaagcctgc
     5521 acctgtttga cctgtgtatc ggtatcgccg gtgccgtgat cgtgcggctg gtggtgtact
     5581 gcaagagcaa gaacgtgaag aaataccgca aaggcatgga gtatggcagc gcccgctggg
     5641 ggacggccaa agacatagcg ccttacatcg accccaagtt tgaaaataat atcctgctca
     5701 cccaaacgga acggcttacc atgaccggca ggcccaaaga ccccaagaca gcgcggaata
     5761 aaaacgtgct ggtgatcggt ggcagcggtt cagggaaaac gagatttttc gtgaaaccca
     5821 acctgatgca atgctttccg accacggact atccgacttc gtttgtggtg acagatccga
     5881 aaggcacttt ggttctggaa accggcagga tgttccagca ggcgggctac cgcattaaaa
     5941 tcctgaatac cattaacttt tccaagtcca tgaagtacaa cccctttgtc tacatccatt
     6001 cggaaaagga cgtgctaaag ctggtaaata ccctgatttc caacaccaag ggcgaggggg
     6061 aaaagtcagc ggaggatttt tgggtgaagt cggaacggtt gttttattcg gccctgatcg
     6121 gctatatctg gtacgaggcc ccagcggagg aaatgaactt ctcaacgctg ctggaaatga
     6181 taaacgccag tgaagcccgc gaggacgacc cggagtttca aagccccgtc gatctcatgt
     6241 ttgagcgttt ggagcagaaa gaccccgacc attttgcggt gcgccagtac aagaaattcc
     6301 tgctgtccgc aggcaagacc cgttcttcca tcctgatttc atgcggtgcc agacttgcgc
     6361 cctttgacat ccgggaggta cgcgagctga tggaggacga cgaaatggag ctggacacca
     6421 tcggggacga aaagacggtg ctgtatctca ttatgagcga tacggacacg acctttaatt
     6481 tcattctggc tatgctccaa agccagctaa tcaacctgct ttgtgaccgg gctgatgatg
     6541 tgtacggcgg gcggctgcct gtccatgtac gcctgatttt ggatgaattt gccaacattg
     6601 gccaaatacc caatttcgat aaactcattg ccaccattcg aagccgtgaa atatcggcct
     6661 ctattatttt gcagagccag tcgcagctaa aagcgattta caaggacgcc gccgagatca
     6721 tttcggataa ctgcgatagt gtgcttttcc ttagtgggcg cggcaaaaat gccaaggaga
     6781 tttccgatgc gctggggaaa gaaacgattg acagtttcaa caccagcgag aaccggggaa
     6841 gccaaacgtc ccacggattg aactatcaaa aattaggaaa ggagttgatg tcccaagacg
     6901 aaattgccgt gatggacggc ggcaagtgta tcttgcagct acggggtgtg cgcccgtttt
     6961 tatcggagaa attcgatatt accaagcatc cccggtacaa ataccttgcc gacgccgaca
     7021 agaagaatac ctttgacgtg gacagatttt tgactatcac gcgccggaaa cggcagcaag
     7081 tggtaacgca ggatgaagta ttcgacctgt atgaaatcga cctgtcggac gaggacgccg
     7141 ccgctgaata aacagcggct tttttcattc caacaacaaa atcaaaaaat cattatgagc
     7201 cggaaaccgg cagaaagagg taaatttatg gcatttttta attccgcagt tactactttg
     7261 cagactatcg tgatcggact gggcggcgct ctgtgcgtat ggggcggcat caatctgttg
     7321 gagggatacg ggcaggacaa tccgggggca aaatcgcagg gtgtcaagca gcttgtcgct
     7381 ggcggcggcg tggccctgat cggtgtcact ctggttcccc tgctgtccgg gctgctgggc
     7441 tgatcctggc ctttgtccct gtccaaaccc ttaactaatc gcctccgcgt cctcccggcc
     7501 agaccgggag ggcgcgggaa aggaggtgct acccgtgatt agtgaaatca ttgaagaatg
     7561 gataaaaggc atcctcatgg acggtatcac gggcaacctg tccgggctgt tcgatacggt
     7621 aaatgccaaa gtcggagaaa tcgccgccga cgtgggacga accccgcagg catggaacag
     7681 cggtattttc aatatgctgc acagcctgtc ggaaaccgtg atcgtcccca tagccggggc
     7741 catactcgcc cttgtcatgt gctatgagct tatccagatg ataacggaaa agaacaatat
     7801 gcacgacttt gacagctcca tgttcttccg ctggatattc aaatccgcgt ttgcaatcct
     7861 gatcgtaacg aacacatgga acatcgtcat gggcgtattc gacgccaccc aaagcgtcgt
     7921 caatcaaagc gccggtgtga ttatcgggca gacatccatt gattttaaca ccctgatacc
     7981 agaccttgaa agccaattgg aggctatgag cgtgggcgct cttttggggc tgtggttcca
     8041 aacccttgtt gtgggcctca ccatgaatat cctttccatc tgcattttcc ttgtgaccta
     8101 tggccgcatg gtggaaatct atgttgtcac gtcgctgggg cctatccccc ttgccacaat
     8161 gggcaacgcg gaatggcgcg gcatgggcca agggtatctg aaatccctgc tggcgctggg
     8221 cttccaagcc tttttaatca tggttgtggt gggtatctac gcggtgctga tacagaatat
     8281 cggttccgct tccgacatat ccggcgcaat ctggggctgt atgggctata cggtgctgct
     8341 gtgcttctgc ctgttcaaga ccggcagtat atcaaaggcc gtattcgggg cgcattgatc
     8401 ccaaaatcca agcatacaca ggaggaactt gtttatttca acgtacaaca tcatttacgc
     8461 tgacccgccg tggcggtatg actgcaaaaa cgtacagggc gcagcggaaa atcactattc
     8521 caccatgagc attgacgagc tgtgcgccct gccggtggaa cggctggcgt ccaaagactg
     8581 cctgctgttt ttgtgggcca cattccccat gctgccggaa gctctgcggt taatcaaagc
     8641 gtggggcttt tcattcaaga ccgtggcttt tgtatggctg aaacagaacc gtaaaagcct
     8701 tacatggttt tacgggctgg gccgctggac aaggggaaac gcggaaatct gcctgctggc
     8761 aacacacggc aagccgaagc gtcgttctgc ctctgtccat cagtttatta tcagcccgat
     8821 tgaacaacac agcaaaaagc cggatgtgac ccgtgaaaaa atcgtagagc tggcgggcga
     8881 tttgccccgc gtggagctgt ttgccagaca gaaaaccccc ggctgggacg tgtgggggaa
     8941 cgaggtggaa tgtgacctta tcctgacgtg aaaggaggtg ccaaatggct tatgtaaccg
     9001 tccccaagga cttaacccat gtgaaaagca aggttgtgtt tgggcttacc aaacggcaac
     9061 tgatctgttt ttccggggcg ctggtgatcg gcgtcccctt atattttctc acccgtgact
     9121 atcttaccaa cagcgcggcg gccctgctca tggtatttgc catgctgccg ggctttttgc
     9181 ttgccatgta cgagcgtcac ggccagcccc ttgaagtggt gattatgcag atcatccagt
     9241 gctgcttcat ccagccaaag gagcgtccct atcagaccga caacacctac accgcccttg
     9301 tgcggcaata ccagatggaa caggaggtaa aggccattgt ccaaaaagga aatcccaaga
     9361 gaaaccgaaa aaacaaagct gacccgcgcc cagcgaaaag aaattgacgc ggtaatccgt
     9421 aaatataagg gcgacggcag gccccacacg gcgcagcaga gcatccccta cgaggtcatg
     9481 tacccggacg gcgtgtgccg ggtatcgccc ggcgtctttt ccaagtgcat tgagtttgcg
     9541 gacatcagct atcagcttgc ccagccggac acgcagacgg ctatctttga aaaactgtgc
     9601 gacctgtata actatgtgga cgcctccatt catatccagt tttctttcct caatcggaaa
     9661 gttgacccgg tgcagtacgc caagagcttt gagatcgcgc cgcaggggga cgacttcgac
     9721 gacatccggg ccgagtacac cggcattctg caaaagcagc ttgcaaacgg caacaacggc
     9781 atggtaaaga ccaaatacct gacattcacc atcgaggccg aaagcgtgaa agcggcgcga
     9841 gccagattga agcgcatcgg ctttgacctg ttaggctatt tcaagagcat gggggccgta
     9901 gcccatgtga tggacggctg ggaacggctg aacctgctgc atggcgtgta ccacccggac
     9961 ggcgagatat tcaattttga ctggaagtgg ctggccccgt ccggcctctc caccaaggat
    10021 tttattgccc cgtcctccct gtgcttcggc aatgcaaaga cattcggcat gggcggcaaa
    10081 tacggcgcgg taagtttcct gcaaatcctg tccccggagc tgtcggacga tatgctggcc
    10141 gacttcctca atacggaaag cggtgtttta gtcaatctcc atgtgcaggc cattgaacag
    10201 accaaggcga tcaagaccat caagcgcaaa attaccgacc ttgacgctat gaagattgcg
    10261 gagcagaaaa aggccgtccg cagcggttac gatatggaca ttcttcccag cgaccttgcc
    10321 acctacggcg aggacgccaa gaagctgctg acaaagttgc agacccgcaa tgaacggctg
    10381 ttccagttga cgttccttgt cctcaacgtg gccgacacca agcaaaagct gaataacgac
    10441 gtgttccaag ccgcaggcgt ggcccaaaag cataactgtc cccttgtccg gctggactac
    10501 cagcaggaac agggccttgc aagcagcctg ccgctgggcg tcaatcagat caagatacag
    10561 cgcagcctta cgacttccag cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt
    10621 cagggcggcg cggccatgta ttacggcatc aacgccaagt cccgcaacat gattatgctg
    10681 gacaggaagc aggcccgttg ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa
    10741 tccatgagct gtaaatccga gatcgtcagt gtgttcttaa cgacgcctga cgatattttt
    10801 atttcagacc cggaggccga atattacccc cttgtcaaac ggctccacgg acaggtaatc
    10861 cggctgtcgc ctaccagcaa ggactttgtg aacccgctgg acatcaacct gaattattcg
    10921 gaggacgata acccgctggc cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc
    10981 atgggcggca agaacgggct ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc
    11041 gtgatctacc ggccctatct ggcagacccc cgcccggaga atatgccgat cctgtccgac
    11101 ctccacaaag cgttgttaga tcagcatgtc ccggaggctg accgggtggc gcaggccctt
    11161 gacctgtatg tgtccggctc cctgaacgta tttaaccacc gtaccaacgt ggacatcggc
    11221 aaccggcttg tgtcctttga cattaaggag ctgggcaagc agctcaaaaa gctggggatg
    11281 ctcattgtcc aagaccagat atgggggcgc gtcacggcca accgaagcca aggaaaggcc
    11341 acatggtatt ttgccgacga gttccatttg cttttgaaag aggaacagac ggcggcctat
    11401 tctgcggaga tttggaagcg gtttaggaaa tggggcggca ttccaacagg tgccacccag
    11461 aacgtcaaag acctgctttc ctccccggaa attgaaaata tccttgaaaa cagcgacttc
    11521 attacgctgc tcaatcaggc gtccggggat agaaaaatcc ttgcggaacg cttgaatttg
    11581 tccaccgaac agcagaagta catcgacaat tccgaacccg gcgagggcct tttgatcttt
    11641 gaaaacgtgg tgctgccctt taccaacccc atcccgcaca acacccagct ttacaagatt
    11701 atgacgaccc gcttaaacga ggtggcggga gtatgacccg gcgacgccac aaaggaggtg
    11761 aaccaccatg aaagagctga aagccaaggc gaaagtgacg caggccatga cccgcgacgg
    11821 gctgacgctg gaaaatcagg ccaccggcga ggtggagaac atttccagcc gggaggccga
    11881 acaggatttg tccgcgccgg ggccgggcgg caccgccgat aagctgctgg aacgggccga
    11941 cgccatccat gacggccata aggccaagaa agccgcgaag caggccgggg ctgtggccgc
    12001 cgagggggac gctgccctgc accggccatc cgcccggctc caactgtcgg aggaagaacg
    12061 ggccgacccg gatttacagc cctatatccg aaaatcggac aaacgggccg ataagctgga
    12121 taaggcccgc gccgccatcc ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga
    12181 ggcaagcggc aaagcaaaga ccgtcctgca ctttgacaaa caggacaagg ggcctccctc
    12241 cctgaaaccc aatccggcag gccgtcctgt ttccgaggtt tttctattcg gccacgggaa
    12301 aatccatgaa gtcgagcatg aaaacgtggg tgtggagggc ggccataagg gggaggaact
    12361 tgccgagcgg caggccggaa aagccatccg cagcaccatc cggcaccaca ggctgaaacc
    12421 ctactgggac gcggaaaagg ccgagcgcag gctattgtct gccaacgcgg agtatttcta
    12481 ccagaaatct ttgcgggata acccggagat cgcacaggcg gccagcaacc ccatttcccg
    12541 gttctggcag aaacagcaga tcaagcgcca gtacgccaag gccgcccgga cagcgggcca
    12601 gaccgcaggc gcggcggcac agggcgcggc caagacagcc ggggcctcca cgaaagcggc
    12661 caaggccgca gccaaagaaa gtgaacgggc ggcctccttt gtggcccgcc actggaaagg
    12721 ggcgctggcc gtccttgtgt tggggctttt gatgctgctt atcatcggcg gcttacagtc
    12781 ctgcaccgcc atgttcggca gcgccggaag cggcatcacg gcgtcctctt acttttccga
    12841 ggatagcgat atgttggccg ccgaggacgc ctacgcggag ctggaagccg agctgcaaga
    12901 ctatctggac aactacgagg ccacccatga ctatgacgaa taccattttg accttgacga
    12961 gatcagccat gacccctatg tgctgatttc cattctgtcg gcgctccatg acgggatatt
    13021 taccatcggg gaggtacagg gtgatttgtc catgctcttt gacaagcaat atatcctgac
    13081 agaaaacgtc gtggtagaaa cccgttaccg caccgaaacc gatacatgga cggacgcgga
    13141 gggcaacacc catacagaca cctaccaagt gccgtatgac tattacatct gctacgtcac
    13201 gctggaaaac ttcgacctgt cccatgtgcc agtctatatc atggacgagg aaacgctaag
    13261 tttctattcc ctgtatatgt ccacgctagg aaaccggcct gatctgtttt ccggcagcca
    13321 gtaccccaac gcctccaccc tgacgccgcc gacctactac gacgtacccc cggaggcttt
    13381 ggaggatgcc acctttgccg ccatgctcaa agaggcggaa aaatatatcg gttatcccta
    13441 tgtgtggggc ggcagctctc ccagcaccag ctttgattgc agcggcttca tttcgtgggt
    13501 aatcaatcat agtggatgga acgtgggacg gctgggggcg cagggcctat ataacatctg
    13561 cacccctgtt tccagttcgc aggccaagcc cggcgacctt gtatttttca agggaaccta
    13621 cgacaccccc ggcgtgtccc acgtcggcct gtatgtgggc aacaatgtga tgctgcattg
    13681 cgggaacccg atttcctaca cgaaccttac ttcaaactac tggcaatccc atttttacag
    13741 ttacgggcgt ttaccttaac gccagaaagg agctttcaat tatggcaacc aaacttgacc
    13801 gcatcgaaaa ggacatccag aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc
    13861 gggagctgga aacgcagaaa accgaacagg agaatttgca gatcatccag cttgtgcgcg
    13921 gcatgaacat gaagccggag gaatttgccg cgttcttacg cagcggcgca atgcaggccg
    13981 cccctgccgc aaccccttac catgaacagg aggacaatgc ccatgaagaa taagaccaga
    14041 cggatttccc gtattctccc cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc
    14101 gttaccgccc ttgcaggcgg caatgacccg gcaccggccc cgctgccgga ggctacaacc
    14161 gaacccgcta cgggcggttt ggagcctgaa acagacgaaa ccggcaccgc cacggacggg
    14221 acagaaaccg aggccgaaaa agataccggc cctctgaccg gcaaggacat caccgacctg
    14281 ttttccgcgc tgttcggttc caaggtgagc attgccgcca cggacgacgg catccagatc
    14341 accaccggca ccgacaaaaa agaacctgaa cagaccggca ccgtcaccac caacggcggc
    14401 aggctgaatg tccgcaccgg cgcggggctt gacaagaccg cgttcaccca gcttcccaat
    14461 gggacgacgg ttgaggtagt cggcacagac ggcgactgga ttaaaattct cttgccggag
    14521 cgcatcggct atgtccattc cgactatatg accgtcagcg aaaaggaagt agcggctacc
    14581 ggcgagggcg gtttttccct ctccattgac ccggaggaaa ttgcttctct gctggaactg
    14641 ttcaatggcg gcggggccag cgccgccctg tccccggacg ggaacctgtc actgattgac
    14701 gacatcggca gcgccgcgaa aaatggcaag cagtttatca ccgtggaaac caaaaacggc
    14761 aatgtgtttt atctgattat tgaccgggac gacgagggaa aagaaactgt ccatttctta
    14821 aatcaggtgg atgaagccga tctgctgacg ctgatggagg acggggaaac cgtcaccccc
    14881 gctgctgtct gctcctgtac cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg
    14941 gtatgtaaaa ccaacctgac cgagtgcagc ggcccggagc cgcaggaacc ccagccggag
    15001 gaacccgaag ccccggagga agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg
    15061 ctggtggcgc tggcgggcgg cggtgcggcc ctttactatt tcaagttcaa aaagcccaag
    15121 gctgaaacca ccggcagcga cgatttgggc gagtatgatt ttggggagga cgaggatttg
    15181 gaggacgagg aacccgaaac cgagtttgac ccggaagctg attttgtgag tgaacaggag
    15241 gacgaggaat gacatataaa cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg
    15301 cgggcgtgat cggggccacg gagaaacacg acggctattt ggaggggaac ggctatctgg
    15361 tgagctggtg catcggacat ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt
    15421 tcaaaaaatg gcgctatgag gatttgccca ttctcccgga aacatggcag tacatcgtcc
    15481 ccgacgacaa gaaacagcag tttgacgttc tctgttcgct gatgcagcgc cccgacgtga
    15541 ccggccttgt gtgcgccacc gacgccgggc gcgaggggga attgattttc cgtttcgtct
    15601 accagatggc gggatgccaa aagccctttg agcgcctttg gatttcctct atggaggaca
    15661 gtgctattaa ggacggcttt gcccacctga aaccgggcgg cgactatgat ccgctgtatc
    15721 aatcggccct gtgccgggcc aaggccgact ggctgatcgg cattaacgcc acccggcttt
    15781 tttccgtcct gtaccacaag aacctgactg tgggccgcgt ccagacgccg accttaaaaa
    15841 tgctggtgga cagggacgcc aagattaccg atttcaagaa agagaaatat cacatcgtcc
    15901 atatcacggc gggcggcgcg gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa
    15961 acaccgtcaa ggcggcttgt gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga
    16021 aaaagacgga acagccgccc aagctctatg accttaccac cttgcagcgg gaggctaacc
    16081 ggcttttcgg ctataccgcc aagcagaccc ttgactatgc ccaggcactg tatctaaaga
    16141 agctgctgac ctatccgaga acggatagtc aatatcttac cgacgatatg ctgcccgccg
    16201 tggaaagcct tgtgtccggc ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt
    16261 ccccgcagtt tgaccgcatc ctgaacagca aaaaggtgtc cgaccaccac gccattatcc
    16321 ccaccgctga atttgtgaag cagggctttg gcactctggc cgagagtgaa aaaaagctgc
    16381 tgtccctgat ctgctgcaag ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg
    16441 tcaccgccac atttacctgt gccgggcagg agtttaccgc caagggcaag accgtccttt
    16501 cccccggctg gaaagagatt gaacgccggt tccgttcttc cttaaagacc gacgccgacg
    16561 aggacgccga ggcggtgcgg gagctgccgg agctttgcga gggccagacc tttgcagacg
    16621 tggccgcctc tgtcaccgag catttcacca cgccgcccaa gccctacacc gaagatacgc
    16681 ttttgtccgc tatggaacgg gccggggccg aggatatgcc ggaggatgcc gagcgcaagg
    16741 gattaggcac accggccacc cgcgccgcca ttctggaaaa gctggtgcag atgggctttg
    16801 tccagagaaa aggcaagcag ctcattccca ccaaggacgg catcaatctg gctgtggtgc
    16861 tgccggaggc gcttacttct ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga
    16921 tcgccaaggg gcaggccgat cctgacgaat ttatggacgg catcgagacg caggcgcggg
    16981 agctggtgca gacctattcc tgtatcagtg aggaaaagca gaaattgttt caggccgagc
    17041 gtgttcctat cgggacgtgt ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact
    17101 actactgcgg aaaccgggcc tgccagttcg tcatgtggaa gaatgaccga ttttttgagg
    17161 aacgcaagaa agcgttcacc ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca
    17221 aggtaaaggg cctctattcc gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg
    17281 ctgataccgg gggcaagtac gtcaactacc gcattgaaca caggagctaa aggacagtaa
    17341 cacagcaagc atagggagtg tggctacact cccggtaaaa tcccccggcc gcgtccctgc
    17401 cgggccgggg atttttgctt gttggggaag tccccaaccc cctatttttt gaaacagaac
    17461 agagaaagga gtgaccgcgt gaacgaagta ttttctatcc aagtccataa ccggcagttt
    17521 ttggaacagg gcaaacaagg cgtctggctg gatttgccca ccacaaccga aacgctgcaa
    17581 gccgccctgc gggatgttgg cattactgcc gacaatccgc aggatttttt tatcaacggg
    17641 tattcctgcc cggaggacag acaccttgcc attccctatg acatggtgct ggccgccgac
    17701 gtggacgagc tgaatttcct tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca
    17761 gagctgaacg ccgccctgca aaatccccgt ggcggctttg agaatatcgg ccagatcatc
    17821 gactacaccg aaaatgtgga ttactatgtg catcttcccg acgttagggg ggatggacag
    17881 ttgggcgact actacctgaa ccgttccggc atggtggata tgccggagga atggaaagcc
    17941 gggattgata cggcgcagtt tggacgccac attgcccagc aggaacaagg cgtttttacc
    18001 cagtacggct atctggtgag aagcggcgac gaatggcagc gcgtccatga gggccagccg
    18061 gtgccggagg aataccgggt gatgcactac ccccagccgg aagctgaccg ggaggaaccg
    18121 caggcccagc agccggaggc atccgaaagc cccgacaaag cgccgccccc ggttatcccg
    18181 atcatgctgg acggcatgaa cagcaaagaa cgcatgaaag tgattactga ccgtctggaa
    18241 accggcgtac aggagctttt tgaaagtagc cgctatatgg cttacttatc cgctatgtcc
    18301 aagatgcacg attacagtct gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt
    18361 gtggcgggct ataacaggtg gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa
    18421 ggcattaaga tactggcccc ggccccgttc aaggttaagc agcagatgga gaaaaccgac
    18481 ccggccaccc agcagcccgt gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa
    18541 gtgacggttc cggcctttaa ggtggtgaca gtctttgaca tcagccagac cgagggcaag
    18601 gattttccca tgccctatat cagtgaactt accggcgacg tgcagcagta tcaggatttc
    18661 tttgccgcgc tggaacggac atcaccctat cccataagca ttgagccgat ttcagacggg
    18721 gccaagggcc gctgcttcta cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa
    18781 ttgcagaccc taaagaccgc catccatgag attgcccatg ccaagctcca cgccattgac
    18841 cgggaggccc ccgccgagga acaggcggcc cggccagacc gcagcacccg cgaggtacag
    18901 gccgaaagcg tggcctatac cgtctgccag tattacgggc tggacacgtc ggactattct
    18961 tttggctatg tggccgattg gagcagcggc agggagttat ccgagctgaa agcctccctt
    19021 gaaaccatcc gggccacggc cagcgaattg ataaccgaga tcgacggcca ttttgcccag
    19081 cttcagcagg agcgcgaggc ccggcaggaa caggaggccg tcacggttga cgttaccccg
    19141 gaggcagcgg ccccggcgca ggccgaggaa gaacaggccc cggcccttga tccggcagcc
    19201 gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg ccagcaaatg
    19261 ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc ggcagaggca
    19321 ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc ggacagctac
    19381 gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat ccagagctac
    19441 caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac aaccggcata
    19501 gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt tgtcccctat
    19561 atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg cttacagtcc
    19621 ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc ttatgtgcag
    19681 gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc gaaactggct
    19741 gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat cgcgcaggag
    19801 gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga agcaccccaa
    19861 caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga ctatcggttt
    19921 gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt
    19981 gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg cacattcaac
    20041 atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt ggtggtgctg
    20101 caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat ggaagtgccg
    20161 gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca gaacgaaaac
    20221 atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga acccgaacag
    20281 ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc cccggaggct
    20341 gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc ccgccgcgcc
    20401 aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga ataccggcac
    20461 tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga gccagagtat
    20521 cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga gaacatgaat
    20581 aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc
    20641 ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga ggaatttcag
    20701 tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat cagcgccgac
    20761 gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc gtcgcaggag
    20821 ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg atgcccccat
    20881 ctgatcgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg gcactatgag
    20941 aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg ccggttaaag
    21001 ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg ggagtttgac
    21061 ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag
    21121 ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc
    21181 ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc
    21241 atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg cagggagaaa
    21301 gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc caagcagcca
    21361 ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg
    21421 gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta tcaaagtgac
    21481 tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag
    21541 accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg tgatgttttc
    21601 ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac ccgtgacaag
    21661 atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg gggcaatctc
    21721 tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca gccggtgagt
    21781 gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca gcgttttgac
    21841 ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc ccatgacccg
    21901 gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc cgtccccggc
    21961 gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga ggcccggcgt
    22021 attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac gcatttcatg
    22081 gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga ccggctcttt
    22141 gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca tggcgtgtat
    22201 gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg gccctctatc
    22261 cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc ggccagaacc
    22321 aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga agaaaacctg
    22381 atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac cgccgtcatg
    22441 ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa gctggaacgc
    22501 atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata acgaacagca
    22561 gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt
    22621 cctacgcatt tagaacccgg tattgttggg gataggatga agtattcgct tgcccctgca
    22681 aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag
    22741 ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg
    22801 tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt
    22861 tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg ccgtatatct
    22921 tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt ggaaagctcg
    22981 ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc accggcaacg
    23041 gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct gatacccccg
    23101 tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga aaacggcgct
    23161 tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag gataaaacac
    23221 ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt tacagctcta
    23281 agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc
    23341 gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac gtggccccgc
    23401 actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac agggccgcat
    23461 ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa atgctctgta
    23521 cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa tagttgcagc
    23581 actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt ggaatactca
    23641 tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca cattctgctt
    23701 gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat acagccgttc
    23761 cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg ggagcttttt
    23821 cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg tgctttcatg
    23881 ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca cgggccagag
    23941 ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc ggtgttccat
    24001 agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca ctttgctttc
    24061 caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg
    24121 cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg ccttaatgtt
    24181 gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc tggtgataac
    24241 gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca atgtcttgca
    24301 gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg gcttgatctc
    24361 atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc
    24421 ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct gggcgtccca
    24481 ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc cgttttcctt
    24541 gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg aaatgtactt
    24601 gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt ggcccttatc
    24661 aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg caagctgctt
    24721 cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg
    24781 gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa attcaatgtc
    24841 cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag ttttgtccgg
    24901 gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta tctttgtaac
    24961 tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta caccggcccc
    25021 ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg tatctcggcg
    25081 gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat gagcatcttc
    25141 cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc aatcatagcc
    25201 cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt tgccatgctt
    25261 caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg gacgcggccc
    25321 ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc ttgctacgtt
    25381 actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa atagccctct
    25441 ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa
    25501 cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca attagtatgg
    25561 cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt ttcagcttgt
    25621 ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta aagtagcgtt
    25681 ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg ggaatggccc
    25741 gtcgttttcg atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt
    25801 gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc
    25861 gggacaatct tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc
    25921 tgcccgtgga atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct
    25981 tttattcttc gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg
    26041 acgttgaaag aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt
    26101 gctgcgggac gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact
    26161 gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat
    26221 gaaaccggcg cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg
    26281 aaaaactata agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg
    26341 aaacagaaag aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga
    26401 aatggaaaat gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag
    26461 aattgaaaag gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc
    26521 tggtctaatg cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt
    26581 ttgaggaaaa tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg
    26641 acacggatga tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt
    26701 attaggactg aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg
    26761 ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt
    26821 tgctgaggct tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt
    26881 ggatcagaat atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt
    26941 ttgacaaccg ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct
    27001 tgatttaatt cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc
    27061 ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag
    27121 actgttatgt tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc
    27181 gaggatgatg atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag
    27241 gtggatgcct gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa
    27301 ctggttattc ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt
    27361 cgtgcgaaaa atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa
    27421 atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt
    27481 ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt
    27541 gtcggcaggc tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg
    27601 cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc
    27661 ttaacgcatg aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc
    27721 acagtccaca ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc
    27781 attcgcggtg taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca
    27841 ttaaggtttt ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac
    27901 tgtttgcaca gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat
    27961 cctatcagcc attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg
    28021 cagggctgtt tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa
    28081 gcgtactcta tgccacacca aatgccaata catcaaatag ttttagaccc aactttcttt
    28141 atgtggtaca tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat
    28201 tgctttatca agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca
    28261 gccttttatg cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg
    28321 acagtgcgaa taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg
    28381 agcttggcgc actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg
    28441 caaggctgga ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct
    28501 ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg
    28561 gaatgcttga aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca
    28621 aaatgatgga taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg
    28681 atgggagaat cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc
    28741 tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag
    28801 ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat
    28861 tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg
    28921 ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc
    28981 caaggctgtt caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa
    29041 gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt acaaaaaacg ctggatgcca
    29101 tgagcctcca gtatgcgctg gaaaacacct cggatggcgt tttgttctgg ctggatttac
    29161 cgctcacatc aacattgtaa atatttaaaa cttaaatgat tttgggctct ttgtcaaata
    29221 ggactgatag agtgagataa gaacatctaa ggtagaattc cttttctact ttagatgttt
    29281 tttgattcat aggtaatcag attatttagt aagaaaattc tggtcttcat tttccgaaag
    29341 cttcgaaagc cataagcgac tcgtttcaat gctttgatgt gggtatgcag attttctatt
//
LOCUS       DANKHJ010000420         9706 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.097, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000420 DANKHJ010000000
VERSION     DANKHJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            complement(<1..504)
                     /locus_tag="PW113_003719"
     CDS             complement(<1..504)
                     /locus_tag="PW113_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527908.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRF"
     gene            1016..1651
                     /locus_tag="PW113_003720"
     CDS             1016..1651
                     /locus_tag="PW113_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2527909.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1993..2628
                     /locus_tag="PW113_003721"
     CDS             1993..2628
                     /locus_tag="PW113_003721"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2527910.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            2735..3973
                     /locus_tag="PW113_003722"
     CDS             2735..3973
                     /locus_tag="PW113_003722"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2527911.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            4200..4619
                     /locus_tag="PW113_003723"
     CDS             4200..4619
                     /locus_tag="PW113_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2527912.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            4624..4863
                     /locus_tag="PW113_003724"
     CDS             4624..4863
                     /locus_tag="PW113_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2527913.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5088..6011)
                     /locus_tag="PW113_003725"
     CDS             complement(5088..6011)
                     /locus_tag="PW113_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2527914.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(6043..6807)
                     /gene="map"
                     /locus_tag="PW113_003726"
     CDS             complement(6043..6807)
                     /gene="map"
                     /locus_tag="PW113_003726"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2527915.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6965..7405
                     /locus_tag="PW113_003727"
     CDS             6965..7405
                     /locus_tag="PW113_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2527916.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(7583..8152)
                     /locus_tag="PW113_003728"
     CDS             complement(7583..8152)
                     /locus_tag="PW113_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2527917.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            8407..9639
                     /locus_tag="PW113_003729"
     CDS             8407..9639
                     /locus_tag="PW113_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2527918.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         3135 a         1697 c         1827 g         3047 t
ORIGIN      
        1 aaaacgtcgg ataacaccgc cacagtcaag acagcggttt aagaatgaat accagtccct
       61 gttctgtgcc tgtaagacat attccatgtt gcgtgagcct gcccctttta attctaaaaa
      121 tacgcccatg tgttcatcat cagaagccat gaccttgatt tccccgtaag cataatgctc
      181 cttatagcca taatacccat agtcataatg gataaaataa tctgctttca tgccaagcac
      241 ctttcggata acttccaatg cgtcagtcgt tggaaaacgg acagacagat aatcaacgag
      301 cagataaaaa ggcttatcgc agatatagta attcagacaa cgcaggataa tgttctgtgt
      361 ttccttgctg gcgttagctt ttccgttctc aaagcggttg atactatatc ttgatacatg
      421 ggtaagactt gcaagttctc cttgtgaaag accgctggca agacgtttgt tcttcatttc
      481 aagtgcaaat tctaaattgt tcattgtatt cctccaatcc cacaatagcc ttgaaatacc
      541 tgtaaaccac ctttcaaaaa gtgtgatatg tactatcttc ttaaaacctc tcattactac
      601 tcacaggttt gggggtgtat ggtttttgac ttgtcttttg tgaaatagtg cccccctgtt
      661 agataacggg ggcacagaca aggctcgcag gctcgccagc cgacaaagca tttcttcccc
      721 ttgacacaag gtgtcatggc ggtaagcctg agaggcttcc gccaccgtgt cagaaatact
      781 ttgtcagcaa gttctgtttt gccttttcta acaagttcca tttcaaccga tattcatatc
      841 tacggattta atcagatagg tatttctttt tgaatgtttt tatatttcat tctattgtgc
      901 ttattcgatt acaagaacat agtaattctt atatgcttat ttgtcaatat ctttttttgc
      961 ctttacaaat catattaagc atgatagact tataatagcg aagatataac gagatatgga
     1021 gggttatgca atgaaaataa gccatttagg aaataatata cagaccataa gaaaatttag
     1081 gggaatgaaa caacaggaac ttgcggacaa aatcggtatc aatatgcaga gcctttccaa
     1141 gattgaaaga gggttgaact atcctgccta tgaaacgctg gaaaagataa tggaagtgct
     1201 ggacgttacg cccaatgaat tgttgtcggg agaatggaag tatgtcaatc aatctgaaaa
     1261 ggaagtctgt cagtttttaa gagccgaaga acgtctaaat gcagaactga aacatggaca
     1321 ctatgataac ttctttgaca gcgaggaaga atggctggaa tatgagttag aaaagttacg
     1381 ggaatatatt acagactata tcaacgggaa aagcattgaa gcgtccgacc tttacccaat
     1441 caaagaattt atccagcatt tgaagtttca aaaattatta gaccgctatg atgatttata
     1501 cagtatggat atgtttgggg aaagcataga gggacataag tataagacac cttatcaagt
     1561 cgtgaaaatg ataaacccga actcaaaaga agatatggaa ctgttacgag aagtattaag
     1621 gaataacaat tttgatgatg aggacgagta acttttttcc tcttgcgaga agatacagca
     1681 aacaaaaagc ccgatcaaga gtgcaaagca ccaccaatgg tggcaaagct cttgacaggg
     1741 ctttttcttt tctgttgtgg gcaatcaaga ggaagaaagg gataaaatct gatttgtggt
     1801 aaatcaggca aaggcaacgc aggtatcaca gtatgaattg acaccgcata aggaaagcaa
     1861 ctggaagatt gtagaataaa tgaaaaaggc ttgcattctg catttttata aaattgcaag
     1921 tctttatatt gacaagcata aaatactaag atatacttta atcagaccaa cggtttgtat
     1981 tggaggtaag ttatggcacg aaataaatat ccagaagaaa cagtaaaact cattttagat
     2041 gtttctacaa aaatgttttg tgaaaaagga tatgataaca catcattgca agatattatc
     2101 aatgaaacaa aactttctaa aggtgctatt tatcatcatt tcgattcaaa ggaagatatt
     2161 ttaaaagcaa tttttcaaag aattggaaat gaaaatgcag taatatttgc taaaattaga
     2221 gataacgata gtttaaacgg tttagaaaag ttaagagaaa tatttaagac agcggtattc
     2281 aattctaacc aaagcgtttt attgacagtt tctcctcaac tattaaacaa tcctcgtttt
     2341 cttgctatgc aaatagaaca aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt
     2401 ttagaacaag gtatcaagga taagtctttg gagattgaaa attcacatga aattgcagaa
     2461 gttatcatgg tattaagtaa tgtatggctc aacccattag ttgaaatgac ggataaagag
     2521 ggtatggaaa aacgttgcaa tacattcaat gcccttttaa atggaatagg catagacaca
     2581 ttattagacg aagaaacaat ttctgcattt atacagtttt gtaaataact gtatttgtta
     2641 tccctatacg atagactgct ctataaaaga gcagtttatt taaaccatta tataaaccga
     2701 cggtctgttt gattaaataa aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa
     2761 tcgtgatttt atacttgttg ttataggaca gataatttct ttatttggaa acgctatcct
     2821 acgatttgca ttaccgtact atttgttaaa agaaactggt tcatcagctt tgtttggtat
     2881 tgctacagcc tgctcttttt tacctatggt tattttgtcg ctggtaggtg gtattattgc
     2941 tgacaggaca aataaaagaa acattatggt catactagat tttgtaacag caatcataat
     3001 tttagtattt tatttgtcat tgggaaaaat tcctatcata cctttgttta ttgttgcttt
     3061 gatgttactt tatggtattt ctggtgcata tcaaccagcg gtacaagcaa gtattcctct
     3121 attggtatca tcagataaac ttaccgtcgg taatgctata ataaatcaag taaatacttt
     3181 agcaaacttt atcggaccag ctataagcgg agtagttttt caattttatg gtatcaatcc
     3241 cattttaatt gttagtatta tatgttttac tttttcagcc attatggaaa tttttatcca
     3301 tattccacac caaaaaagac ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt
     3361 aaaagatagt tatacctttt tgaaaaagga gaaacccatt tttatatccg taatcttttt
     3421 ggtttgcatt ttcaatttgg ttttaagtgc tgttttaatt gttggtctgc ctatcttaat
     3481 ttctgaagtt ttaaaaatgc cagatacact atatggtttt tcacaatcag cattatcatt
     3541 aggtggttta tgtggtggta ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa
     3601 cctttatacc ttgttactta tttgttctgt ttctgtttta cttatgggaa tttcgctatt
     3661 tttagaattg cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat
     3721 gggtgtatct actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc
     3781 gcaattagta ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat
     3841 tggacaaatg gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat
     3901 gtttgtagct tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa
     3961 gttagagata taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga
     4021 aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta
     4081 aggctcgcac aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta
     4141 aagggtataa acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta
     4201 tgaggacatc ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg
     4261 tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag
     4321 tattgttttg tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt
     4381 tttatgattg tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac
     4441 ttggtacagt tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt
     4501 ttcaaggaat gaacgacaga gaaatttcag aactgtacca tgtatcacgt tctgctattt
     4561 gcagtagaag aactagagga ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa
     4621 gaaatgagta aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga
     4681 gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc
     4741 ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta
     4801 aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag
     4861 taatcaactg tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt
     4921 ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc
     4981 tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa
     5041 atttcaaata aattactgat ataaatttac aataagaact cttgtttcta ctttttttta
     5101 tgagaaaatt tagttttcaa tttattgaac cattttcggc ttggatctcg acgttttttg
     5161 atttgatgac ccgtcacgta ttcttgaaca actgcattga tgattccgcc caaaatgatg
     5221 atcgtagcag caaaattaag ccaaagcatg agtacaataa aacttcctat gatctgataa
     5281 ccgctgatcc tcgttgcaaa atacctagca tagatactaa acacttgtga taacagcatc
     5341 cagccgacag tagcgaagat cgtgcccgga atgatcgtct tcaacttcaa ctgagcatta
     5401 gggacaatta aataaatcaa gaacatgatg acaaataaag ctaccaatgt gattggccac
     5461 ttcaaagttt ggaactgatc gataaagttc acgggtattt gtacgattgg ctgaatagca
     5521 tccaaaatca gctgtcccaa cccaagaacg atagttaccc cactaatagc aatcatgaac
     5581 aagacaatga caattagtga gaagaaacgg acgatcaaaa aattttttct attttctacg
     5641 ccatacgctt tattcatcgc aatttgcaaa gcattgatac tttgacttgc agaccataaa
     5701 gccgctaatg cagaaacgga taacaagcct cctgaacttt gtgtcaaaag gtctttgatt
     5761 gcaggaccga taaactggta aacggtctca ggaatcactt ctcgtaaata ggtcagtaca
     5821 gtctcttgat cgatgtggag aaacggtaaa atattaccaa aagcaataat caatggaaat
     5881 aaagacaaga gtaaataata agcaacgacg acagaggtcg tactcatttc tgaatcagtg
     5941 atgcgctttt gagtcgtctc aataaagcgc atcagatttt cattttgttt tattttgttc
     6001 caaactttca tgtacctctc ccgtacctat tgactggata gatcaataag tgccttcttc
     6061 tccttgtgat gtcaaaattc ttggaccatc tttagtaata gccaatgtat gttcatattg
     6121 gcaagaaagt ccgccgtctc ttgtatacgc agtccagccg ttaggatcca ttttcatttt
     6181 ccaagttccg gtattaacca taggttcgat cgtgataacc atgccttctt tcaaccgcaa
     6241 cccttttcca gcttctccgt aatgaggaac tgctggttct tcatgaatcg taggaccaac
     6301 gccatgacca ataaaatcac gaacgatcgc tagattttcg ccttctacat aagtttggat
     6361 agcatgaccg atgtcaccaa tccgattccc tacttgtgct tgctcaatac cgatgtacaa
     6421 agctttcttc gttacttcca tcaatcggtc gatctcaggt gtagattctc caactacata
     6481 ggcccaacaa gaatcagaaa tgccgccttt caaatcgata cacatatcga cttttaccaa
     6541 atcaccgttt ttcaaaggct ttttacgagg gaatccatga caaatttcat catttatact
     6601 gatacaagtc gcatattcat acccttcaaa gccaatttgc gcagcaattc cgccatgact
     6661 ctcgatatag tttctgacaa atacctcaat atcccaactt gtgattcctg gttttataaa
     6721 atcacgcaga tttttatgta catctgctaa taaagctcca gattctgcca tcatttcaat
     6781 ttcacgtgct gattttaaag taatcatatt ttcgtctcct ttttacaaat ccctctcatt
     6841 ttaacatagc ggcatacatt tagccaaatg acattcccca ggtgtctcta ggttttattt
     6901 tcttttcaat gatgttttgg tataattcat aattgttaaa taattataaa cgaggaggca
     6961 catcatggct ttagcaaaaa tcgtatatgc tagcatgaca ggaaacactg aagaaatcgc
     7021 cgatattgtt gcagaagcat ttgaaaatct ggatatcgaa gtcgaaatca acgaatgtac
     7081 ccaagtagat gcagatgaat tcgaggaggc agatatttgc gtagtagcta cttatacata
     7141 cgatgatggc gatctgcctg atgagatcgt agacttttat gaagacttgc aagaattgga
     7201 tctattggga aaaatttacg gtgtctgcgg atctggtgat actttttacg atgaattctg
     7261 caaatcggtg gatgatttcg atgctgcttt tgcaaaaaca ggcgcatcaa aaggagccga
     7321 aaatgttaaa gtggatctga atgctgaaga agaagatatc gaaaatttag aagctttcgc
     7381 taaagaacta gtcgcaaaaa tttaatatac ttgcaataaa agagccatcc agatttattc
     7441 aatctgtatg gctcttttat tcattgcatt catggaagag ttcttcggga gttaaaaatg
     7501 catacgctgt aagaaaaacg aacttttcct tattttgaca catctcttgt tacctactga
     7561 tttgtggaac ttttgagtat attcaatcga tttcgattgg ttctttgaat ttcaagatag
     7621 catctatact gtcttggatc atcttttcaa tctgttcatc agttggatgg atgatgcttg
     7681 aagacatcaa agctgcgatc ccagtagcta tgatccatgt cccattatac aaagattcga
     7741 tctgctcttc tgaaagattc acataagctg gatcgcgttt cactgtattc acaaagtaag
     7801 tataagagaa attctgcatc tcttttcctc caccatattc ttctaagtac agtgaacgat
     7861 acaacttctt ctctcttctt gcaaaatgga tataattaaa agcaagatcg ataatcgtat
     7921 tccctgtatg ctcaacagtg aacacttcat attgtaaatg atcatagatc tgctcaaaaa
     7981 gagcttgtct caagtcatcc atatttttaa actcaagata aatcggttgc gtggaacaat
     8041 tcattttgtt cgcgatgttt ctcgcagtaa atcgtgtaaa tccatcggtg gcagctactt
     8101 cataagcagc tgccaaaatc tgttctctag taatcgtctt ttttcttgcc atatttttta
     8161 atctccttaa tttttttata acgcttccac cattgattaa tttcattgta acatgttttt
     8221 tttgatatgc aaaattagtt tttgattttc ttcacaagct gaaatagcaa gtagtaacat
     8281 atatcatgaa acttaacctt aaaaaaataa cctccttttt ttatttaaag cctcattgtt
     8341 tgaatcataa ttaaatggta tcatgctaat agttaccaga aaaaatggtg aggaggaatt
     8401 tttattatgt tatctgattt taatgaactt ctaaaaaaat atgcacgctt aattgcagaa
     8461 actggcgtag ccacagaaaa aggacacaca gttgttttac agatcagcgt ggaacaggca
     8521 ccccttgctc gattgatcac acaagaagct tataaactag gagccgctga ggttatcgtt
     8581 caatggactg acgaccagat ccaaagagaa tttcttttac atgctgcaac ggatcgtatt
     8641 gagaatgtac cacaatctaa aatcgatcaa gcggatgaat ggcttgaaaa aggagcaagc
     8701 aggatcagcg ttgtttctgc cgatcccgat gcatttgctg gtgtagatag ccatcgagta
     8761 gcgtcttatc aatcagcgaa tggcaaagca ctaatgaatt tacgcaaggc aactcaagca
     8821 aataaagtca gttggactgt cgtggctgcg gctggtaaac aatgggcagc aaaagtcttc
     8881 ccagaactgc cagaagaaaa acaagttgag gcattatggg atcagatttt caaaacgact
     8941 cgtgtctatg aagaagaccc tgtcttagca tggaagaagc atgatgaaaa attagcaaaa
     9001 aaagcagaag aattgaatcg agaacaattt tctgcccttc actatactgc tcccggtaca
     9061 gatatcatta tcggattgcc taaaaaccat ctttgggaag gcgctggaag ctataatgct
     9121 cgtggagaaa aattcatggc aaatatgccg acggaagaag tgttcacagc acctgacagt
     9181 catcgagtag atggttacat ttcaagtaca aagccactga gttatgctgg caccatcatc
     9241 tcgggtatga aatttacttt taaagatgga aaagttgttg atttttctgc cgaacaagga
     9301 gaagatgtct tagcaaaact gcttgataca gatgaaggtg cgcgtcgttt aggagaagtc
     9361 gcacttgtac ctgacccttc acctatttct caatctggca tcatcttttt caatacatta
     9421 tttgatgaaa acgcatcgaa tcacttagca ctaggttctg cttatgcctt cagtgtgaaa
     9481 ggcggtacag aaatgtccga tgaagaatta gcagaagctg gattgaatag aagtcaaacc
     9541 cacgtagatt tcatggtagg atctgacaag atggatatcg acggaatccg tgaagatggc
     9601 tctacagtac cgattttccg aaatggcgat tgggcttgag tatactttcg cacacatgca
     9661 aaaaataatg gctctttgtc aataaggact gatgagttgt gcaaaa
//
LOCUS       DANKHJ010000421         5873 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.145, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000421 DANKHJ010000000
VERSION     DANKHJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            147..1043
                     /locus_tag="PW113_003730"
     CDS             147..1043
                     /locus_tag="PW113_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2527919.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1193..2134)
                     /locus_tag="PW113_003731"
     CDS             complement(1193..2134)
                     /locus_tag="PW113_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2527920.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2260..3381
                     /locus_tag="PW113_003732"
     CDS             2260..3381
                     /locus_tag="PW113_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2527921.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3639..5141
                     /gene="eat(A)"
                     /locus_tag="PW113_003733"
     CDS             3639..5141
                     /gene="eat(A)"
                     /locus_tag="PW113_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2527922.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5169..5735
                     /locus_tag="PW113_003734"
     CDS             5169..5735
                     /locus_tag="PW113_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2527923.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1930 a         1031 c         1227 g         1685 t
ORIGIN      
        1 gaattttaat agaagaatga tttatttcat catattttca tacaaatgtt aaattttcat
       61 acaaatatag gaaacgtttt actggctttt tactatttaa acaggctatc ataggaaaaa
      121 gtgacagaga aagtgaggcg gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa
      181 ataaatcact ttattttttg ccttccatcc gctctttaga tgtttggtcc ggaaaagatt
      241 tactacagtc ggaattcatt aaatcagcta gacaacagtt ggcaaattat cagctgatcg
      301 tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgccca tttaccaatg
      361 caaaaattgt gttatttttt tggaatcact tcaaagcaga acacgaggcg atgctgaagc
      421 aggcacaaga tgaaccggca atcgatgaga tttatcattt tgattttttg gaagcaaaag
      481 aacttgggct gaaacataat agttcatttt attcgaaaca tatgaatttt tcttctagcg
      541 aaccaaagac ggatctattt tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa
      601 gctataagca ggaatttata aaaagagatg ttacgaccaa ctatttcatt cttccacatc
      661 gagggaatga acaacctgga tatcttactt atgaagagta cctgaaaaaa acaggggaat
      721 cgcgcgggat tctggaactg ttgcgggagg gtcagcaagg agtgactttg cggacatttg
      781 aatccatgta ttttcaaaag aaactagtga cggataatca agcgattgct cattatcatt
      841 tttatcgtcc tgaaaatatt tttttgcttc gagaacgtga tttagatgaa ttaccggaat
      901 ttctgcatac accatttaag ccaatcctat cagagctatt ggaattttat gatgcagaaa
      961 attgggcaaa acgatttatc actaacgatt ccactattta tgagcaatac gaatatgacc
     1021 tctcctctgt tgaaaacaaa taaaaaacct acattttgaa gagattatgt actgaaacaa
     1081 acaagcacaa ttcttatggc atcatgagag ttgtgttttc tttttgatct tttattgggc
     1141 ttaaaaaaag agaaccagct gctgattcag ctgattctca tcagagagcc ttttattctg
     1201 cgccaaggat tccttctttt gcatggacta attgggtcaa aaaggcacaa tatggtgtag
     1261 caatatcata tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg atttcagtca
     1321 aacgatggtt tttgatcagg tcttggtaca tagatgggta atgcaacccg atgttgtcag
     1381 gatcataagt agaagcaata tgttggtaca cttcttcttg atctagtatg attccttctt
     1441 ttgctgcgat agaagcaaat tcagatacga ttgttcgaac catgctctct gccgcttttt
     1501 gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca taaaccattc aatgttccat
     1561 ttacacaagc ttttcgccaa atggaataac gaacgttatg gctatagatc ggatttaaat
     1621 ttgcttcttg aaagacttct acaacttttt ttgtaaatgc ttcgccttct ggttccaagt
     1681 tctctagttc gatttctcca tcacctaaca atgtgacttt tccgggacca gctagaccag
     1741 ctgtccacat cgtgatccca aataagatat ttttctttgg cacatatttt tccagtacat
     1801 cttcatgacc taatccattt agtaaacaca aaacataagt attgtcagta ataatggatt
     1861 ggatcgaaca aaacatatca tctaattgat tggcttttgt taatgctacg atcaagtcca
     1921 cgtgttcgtt gctttcgatg atttcttctg gtgaatagat tggcagtttt gcgaccactt
     1981 ctttaccgtt aaagtcagca atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc
     2041 aacgatcgat caagaggacc tcatttccgc tttgatgtag catcaatccg attcggctgc
     2101 ccattgctcc ggccccagct attgcgattt tcatgttttt tctctccttt attcaatgag
     2161 ttccctttta gtatatagaa aaatggaaga aaatagcatt gttttgtttg aaaaaaatga
     2221 aaaaaagtgt cttacttaag aagaaagtga gggatacaga tggatatttt ggcggcaatt
     2281 gattcattta aaggaagtgc ttcttcattt gaactaaatc aggcagttct aaatggctgg
     2341 gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag atggcggaga aaatactgca
     2401 gaagcaatct attacgcgct caggggaaca tggcgtacca ttgatgggat ggatctctta
     2461 ttccgtcagc gaaaaatcag ctatttattg acaacttggc aaggaaaaaa caccgcagtc
     2521 atcgaatctg cacaagcatt ggggttagat ctacttgata aaccaacgga tcaaactatc
     2581 cgacttgctt ccagttatgc cttaggagaa cttattaaag atgcactgct tcaagaagta
     2641 gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg atggtggtct aggattactc
     2701 caatccttgg gtgcttcttt agaagggtac acagaaggaa atcctttatt atctactaaa
     2761 aaacttcatt ttgatcgaag aaacgttctt gtttctaaag taaaattgat tatcgctgca
     2821 gatgttacca atccatatac aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa
     2881 ggaggaatag atacgacgct agctttctta gataaacaag caggaaagat tgctcagcaa
     2941 ataaaagatt tctgggaggt ggatttagat gatattccag gaagtggagc agcaggcggt
     3001 attggtggag cccttttttt gttgggagcg aagatggttt ctggtttcga tttagtcagc
     3061 caaatcactg gattggaaaa aaagatcgaa caagcagatg tcatcattac aggagaaggg
     3121 cggatcgatc agcaaacgat ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa
     3181 aaatatcgaa aaccaataat cgctatttgc ggaagtagag accaagagct agaaggactt
     3241 tctgattttc tcccaattgt cttaagtatt catttgggtc caataggttt agaagaagca
     3301 atggaacaca ggcaaacatt agaaaaaaca aaaatcttag gacagacact tagcagattt
     3361 ttcaccatcc tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa
     3421 tataggacaa agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat
     3481 tcagactgta ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt
     3541 ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg catcctcttt agccagttct
     3601 gaaaatagga ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa
     3661 atctgacatt cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt
     3721 ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac
     3781 tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg
     3841 cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata
     3901 ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg
     3961 aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg
     4021 cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg
     4081 atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg
     4141 tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa
     4201 aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc
     4261 gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga
     4321 aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac
     4381 aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg
     4441 ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg
     4501 agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag
     4561 cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt
     4621 tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta
     4681 acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta
     4741 taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag
     4801 acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt
     4861 tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga
     4921 tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac
     4981 tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac
     5041 aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg
     5101 ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac
     5161 aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa
     5221 cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc
     5281 cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc
     5341 ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag
     5401 cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt
     5461 ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt
     5521 tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt
     5581 tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct
     5641 tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca
     5701 tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa
     5761 aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga caattctttt
     5821 gataatataa actcaacctt tagaaatgaa atttaattat tttaataaaa gta
//
LOCUS       DANKHJ010000422         5481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.151, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000422 DANKHJ010000000
VERSION     DANKHJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1723
                     /locus_tag="PW113_003735"
     CDS             <1..1723
                     /locus_tag="PW113_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2527924.1"
                     /translation="KEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQE
                     NIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLD
                     SIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSK
                     IVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLL
                     EVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMI
                     DPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNE
                     FVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILA
                     TQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENK
                     PIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKA
                     LVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSET
                     LPDDNDM"
     gene            1953..3020
                     /gene="rlmN"
                     /locus_tag="PW113_003736"
     CDS             1953..3020
                     /gene="rlmN"
                     /locus_tag="PW113_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2527925.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3213..4316)
                     /locus_tag="PW113_003737"
     CDS             complement(3213..4316)
                     /locus_tag="PW113_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2527926.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4868..5416
                     /gene="aac(6')"
                     /locus_tag="PW113_003738"
     CDS             4868..5416
                     /gene="aac(6')"
                     /locus_tag="PW113_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2527927.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1831 a          952 c         1168 g         1530 t
ORIGIN      
        1 aaaagaagct gcagaatatg agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc
       61 tgatgaatgg caggaaccag aaaatacaga gccggagcag ctaagttttg ttccaatcga
      121 cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa ccagtaccag atacaccaaa
      181 acagaccaat acagcagaag gtttttcaga tgagttgcca gaggatgacg gtacctcatt
      241 agaatttgag attgaagcag aacaggaaaa tcaagactat gaattaccga cagttgattt
      301 gcttgattct ataccaactg tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat
      361 cggcgtatta gaacaaactt tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag
      421 cttaggacca tccgtcacaa aattcgaagt ccagccggct gtaggggtaa aagtaagtaa
      481 aattgtgaac ttaacagatg atatcgcgct tgctttagcg gcgaaagatg tccggatgga
      541 agcaccgatt ccaggtaaat cactgattgg tattgaagta ccaaatggaa agatcagtat
      601 ggtttccttt agagaaatca tcgaagcaca accaaaccac ccagataaat tactagaagt
      661 acctttagga cgtgatgttt ctggacgggt acaaacggct gatctttcga agatgcctca
      721 cttactcgtg gctggttcga ctggtagtgg gaagtcagta gcaattaatg ggattatcac
      781 aagtatatta atgcgtgcta agccccatga agtaaaactg atgatgatcg atccgaaaat
      841 ggttgaattg aatatgtaca acggcatacc tcatctgttg acgccggtcg tgacgaaccc
      901 tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa atggaagaac gatatgaaaa
      961 attcgcggca accggagtaa gaaatatttc tggttacaat gaatttgttc aacagaaaaa
     1021 cttggaaaac ggaacgaaac atccaacctt accattcatc gtagtgatcg tagatgaatt
     1081 agctgatttg atgatggtag ccagcaatga agttgaagat gcgattattc gtttagcgca
     1141 aatggctcgg gcagcgggga ttcatatgat cctagctact caacgtccaa gtgtggatgt
     1201 tatcacagga atcatcaaag caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg
     1261 tacagattct cggacgatca ttgattctaa tggggcagaa aagctcttag ggcgaggaga
     1321 tatgctattc ttgccaatgg gtgagaataa accgatccgt gtgcaaggag cattcatttc
     1381 tgaccacgaa gtagaacggg tcgttcaatt tgttacagat cagcaagaag ctcattatga
     1441 agagaaaatg atgccgacag atgaagttga aacgacaggt gctccagaac agccacaaga
     1501 tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg caaacggcaa gtatttccct
     1561 tctccaaaga agatttcgga tcggttacaa ccgagcagca cgtttagtag atgaactgga
     1621 agctcatgga gttgttggcc cgtcagaagg aagcaagcct cggaaagtat tcattgagca
     1681 ggaagaaaca tcagaaacgt tgccggacga taatgacatg taaaacttgt gtgatgaagg
     1741 gttgaaaaac gaataaccaa taaaaagaca aggcatgatg ataccaaaag aaaaaatggt
     1801 ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa
     1861 agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt
     1921 agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga
     1981 cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc
     2041 aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat
     2101 cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg
     2161 tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca
     2221 tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag
     2281 taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc
     2341 tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca
     2401 atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt
     2461 acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg
     2521 ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata
     2581 acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat
     2641 cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt
     2701 atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg
     2761 atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat
     2821 atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa
     2881 aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac
     2941 gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga
     3001 aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca
     3061 gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct
     3121 ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg
     3181 acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg
     3241 ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg
     3301 gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct
     3361 tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt
     3421 ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc
     3481 agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc
     3541 gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa
     3601 accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga
     3661 tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt
     3721 tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa
     3781 gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt
     3841 gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag
     3901 agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag
     3961 ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt
     4021 acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt
     4081 tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg
     4141 gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt
     4201 gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct
     4261 ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc
     4321 atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg
     4381 gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa
     4441 caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga
     4501 caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt
     4561 atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat
     4621 aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc
     4681 ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt
     4741 ttattttata agtaagataa ataatgaaag aatagagata ctgattggta gctaaagatt
     4801 tttgagcgag aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg
     4861 aagacgtatg ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc
     4921 tgatttactg agactgactt ggccggaaga atatggagac agctcggcag aagaagtaga
     4981 agaaatgatg aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg
     5041 atttattggt gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt
     5101 agaaagctcc cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga
     5161 agtagcttcc agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac
     5221 aacgttaagt caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa
     5281 ccttcgtgaa catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt
     5341 accaaatgca aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg
     5401 accaaattct caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga
     5461 aggaatttac gaaaattact t
//
LOCUS       DANKHJ010000423         4759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.164, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000423 DANKHJ010000000
VERSION     DANKHJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            251..1057
                     /locus_tag="PW113_003739"
     CDS             251..1057
                     /locus_tag="PW113_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDL2527928.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1075..1782
                     /locus_tag="PW113_003740"
     CDS             1075..1782
                     /locus_tag="PW113_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL2527929.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1779..2750
                     /locus_tag="PW113_003741"
     CDS             1779..2750
                     /locus_tag="PW113_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL2527930.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2743..3771
                     /gene="vanB"
                     /locus_tag="PW113_003742"
     CDS             2743..3771
                     /gene="vanB"
                     /locus_tag="PW113_003742"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL2527931.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3777..4385
                     /locus_tag="PW113_003743"
     CDS             3777..4385
                     /locus_tag="PW113_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL2527932.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4451..4624)
                     /locus_tag="PW113_003744"
     CDS             complement(4451..4624)
                     /locus_tag="PW113_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527933.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1342 a          996 c         1256 g         1165 t
ORIGIN      
        1 tgattcaatt ctaacggagc ttctgtatgt tttgtttttt catgctcaaa agggaccgat
       61 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
      121 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
      181 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
      241 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
      301 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
      361 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
      421 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
      481 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
      541 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
      601 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
      661 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
      721 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
      781 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
      841 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
      901 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
      961 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
     1021 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
     1081 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
     1141 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
     1201 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
     1261 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
     1321 aacggcttat tgataagacc gggggaaacc ttttctttct ggttgttcct gcatacgcca
     1381 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
     1441 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
     1501 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
     1561 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
     1621 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
     1681 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
     1741 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
     1801 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
     1861 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
     1921 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
     1981 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
     2041 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
     2101 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
     2161 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
     2221 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
     2281 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
     2341 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
     2401 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
     2461 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
     2521 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
     2581 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
     2641 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
     2701 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
     2761 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
     2821 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
     2881 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
     2941 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
     3001 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
     3061 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
     3121 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
     3181 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
     3241 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
     3301 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
     3361 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
     3421 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
     3481 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
     3541 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
     3601 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
     3661 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
     3721 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
     3781 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
     3841 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
     3901 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
     3961 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
     4021 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
     4081 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
     4141 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
     4201 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
     4261 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
     4321 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
     4381 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
     4441 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
     4501 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
     4561 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
     4621 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
     4681 taatccactt ccaccactca aaaaaagcct tgcccctcca tgaactgaac cccaaaagtt
     4741 gaactattta atggactgt
//
LOCUS       DANKHJ010000424         4653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.169, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000424 DANKHJ010000000
VERSION     DANKHJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            143..640
                     /gene="dfrG"
                     /locus_tag="PW113_003745"
     CDS             143..640
                     /gene="dfrG"
                     /locus_tag="PW113_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2527934.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(712..2664)
                     /locus_tag="PW113_003746"
     CDS             complement(712..2664)
                     /locus_tag="PW113_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2527935.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2671..2907)
                     /locus_tag="PW113_003747"
     CDS             complement(2671..2907)
                     /locus_tag="PW113_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527936.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3309..3599
                     /locus_tag="PW113_003748"
     CDS             3309..3599
                     /locus_tag="PW113_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2527937.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            4313..4615
                     /locus_tag="PW113_003749"
                     /pseudo
     CDS             4313..4615
                     /locus_tag="PW113_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1483 a          758 c          817 g         1595 t
ORIGIN      
        1 aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg cttattcaac
       61 taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag atggtaaatt
      121 agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg ataagaatag
      181 agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg aatatgttaa
      241 aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat caatcggaag
      301 agccttacct gacagaagaa atattattct gacgagagat aaggggttta cctttaatgg
      361 ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg aagaagaaat
      421 ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg agaaaatgta
      481 catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag tgaattatga
      541 ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc cgtataacta
      601 ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt aattgaagaa
      661 gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt gtcatttgaa
      721 ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt gagcaggtgt
      781 caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag ttcttagaat
      841 tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc tcttcttatc
      901 accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta tctgttgaat
      961 aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt cgtttggttc
     1021 cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct tagtggctaa
     1081 tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt tactaatagc
     1141 ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg ccaaagtccg
     1201 tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg cttttgcttg
     1261 tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc aaataaaatg
     1321 atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt ttctaataga
     1381 gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt taattaacca
     1441 atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa atctactcct
     1501 attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat cgttagcgga
     1561 tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa aagtattgag
     1621 atggtcttct ttgaattttc ttgtatcttc atttaattca tccattgaaa tattccaatc
     1681 ataggcaaca tcagaacgga acacatatct tgataagacc tcagcagata caactatata
     1741 cgtttgcata ttaaggtctt caaaccccgt atatttcttt gaaccttgtc ctttcttacg
     1801 gacgttattt aggtagtagt gcatcttgtc cgtattaagc cacatctcgt taaatttctt
     1861 tgtttgaagg ggttgtgtct catatcgttc taaaaactcc aaacaacgcc tatacagcca
     1921 ctccagctta tgatagtagg ttccaactcc aattcctaaa atatcacaag tacgactgac
     1981 tgaaaccctt ccaacaacca tcttagcgaa cattggaaga atggtgttct tttgttggtg
     2041 ataagtagtg gtttcttctc tttttggtag aacattagtg aacttcttac acgccttaca
     2101 ttgatagcgt tgagatttgc ccctactttt cccccttttg taaaattgct tcggctcatt
     2161 gaatggggtt gattcttctt ccgagcagcc ttctttatga aattgataat ctggctcaac
     2221 atcttggatg ctattgattt ctataaggcg tttaatttct tcaacaacag accaattaga
     2281 taaaggggtt actgtacagt tttgcgatac acctctgcca ataggattgt cattgcagta
     2341 cataccttta tgacctttat ctttggaact accagtcatt ttatatcgac ttggtttccc
     2401 ttttacatct ttgaatttgt gctgttcttt cccaaaatta caacaaaacg gattgatgca
     2461 gtaattatat tgaatgggat attcagaacc gttccattta aaagacgttg gcaagaacaa
     2521 cattttgtta taccttgttg caaatcgttt tggagttaat aaatcatatt gtttagccct
     2581 atcttttaat tcttcgtttg aaatcggaat tttaacatct acaatttcat cttccttcgt
     2641 ggctaatctt ttaagcttgg tcattgtcta tcaacccact ctatctttga tacccatttt
     2701 ttcaacaatc cgcttattac ttcgcttgtt ggcaatccat tcaataaact tcttatcgtc
     2761 taaaatgttc aataagaatt catccactgc ttcgctttca gtacgctctg catattcagc
     2821 gtattgctta actatcaccc ttacttgctc tgatatttcc caatccacag acttagcgtt
     2881 cttatttttt ggctcaagaa attgcatagg aaaatgcacc cctttttgct tagtaaattg
     2941 taaccatttt aacacaccaa tttgcattcc tacaataatt atttcacgtt cgcattttta
     3001 tatacatttt aacttacatt ttgtggtgta aattaacaac aaacgttgat ttaacaataa
     3061 aaaacgagta tcaaattcag ttgcaatttg atactcattt aggtttacat tttcactttt
     3121 tgtactccaa aactgaacta cttaaatcta tactgctgcc tctttttttt agtacatgtc
     3181 cttcatcttc gcaggaagtg ccaatatgat tttaagaaag gtaatagaca aaaagtcaaa
     3241 aagataaaac agagtttctt ctttacaaag ggaatgtatg ttcgtataat ggtattgagg
     3301 tgaaggttat ggagaaaaaa tatggaacag tagataagat ccgtactttg caagttagtg
     3361 ccagaccgtt gatccgtttc agtttaaatg gggtcaattg tttgattgct tcccatagtt
     3421 tgaacttcct agctgaagta gacgaaggga tgaagttagt cgttacaggt tattataata
     3481 atagaaagca atttgttgtg agatcctatc atgtgttagg gaaacccaaa attgtggtag
     3541 aatatgagaa aagtctatac ccaagaaaaa aggtccgaca agatgaaaga ataaactaga
     3601 aaacaagcgg ctgagacaaa aatgtcctag ccgcttgttt tggaaagatg gatgcagacg
     3661 atgggatttg aacccacacg agcatacgct cacaagaacc tcaatctata tataactaca
     3721 tgtatttaag tatttgtgat gaatcactaa actttccaaa ctcatttgag ggatattgct
     3781 taaagattga acagatcaat tcgaagccct attagtattc gctcaatggg gggatcagag
     3841 gccataaaaa gagactagtc attactagtc tcaggtagtg gaattgtagg attttaaacg
     3901 ttcctggaga gtaccggtat gtaactcgaa gaggtgatta tcatagtcgt aaaagtaaat
     3961 tgaagaagcc tcgcctttca cacgagaacg tcctggttcg acagttaaac ctaacatttg
     4021 gatttttgag acaaacgaat ctatatcttg ttcgtcaatt ttaaaagcaa tatgattgta
     4081 tgtctttggc agtttattcg acgaattctg cattactgca atccataggt ccttaactaa
     4141 gaaaaatttt tctggatata atgaaaattt ttttgttgac tatcgtagat ttcttttgca
     4201 tcaaataagg tatttaacaa ttaagtattt aaaagataac ttttaagtta tctttaagta
     4261 atgcttaaag ctatgttata ctctattagg aaatataaga aggagtataa aaatgaacaa
     4321 acataataga aaaataacga ataacaaaga actgatcttg ttactaatag ggatttgggg
     4381 tatcttttta ctctttaata tgtatatgcc gactatgaga gcggatgacg tagtttatgc
     4441 aagtagatta gatgaacttg gctatttggg tgcttcgata gaacattata agacatggag
     4501 ttcaagaatt attatagaac tattcttaat gttcttttct aaacacttta tgctatggaa
     4561 attattaaat tctacgatta tgttaggaat tgtagtatta ctttgcaagt atgttgagag
     4621 tataaaatat tttgtgtaaa tgaaaaaatc cat
//
LOCUS       DANKHJ010000425         3793 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.188, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000425 DANKHJ010000000
VERSION     DANKHJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            110..193
                     /locus_tag="PW113_003750"
     CDS             110..193
                     /locus_tag="PW113_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL2527938.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="PW113_003751"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="PW113_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2527939.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="PW113_003752"
     CDS             complement(1318..2181)
                     /locus_tag="PW113_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2527940.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="PW113_003753"
     CDS             complement(2183..2455)
                     /locus_tag="PW113_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2527941.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="PW113_003754"
     CDS             complement(2472..2681)
                     /locus_tag="PW113_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2527942.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="PW113_003755"
     CDS             complement(2759..3655)
                     /locus_tag="PW113_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2527943.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          661 c          586 g         1372 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tcc
//
LOCUS       DANKHJ010000426         3121 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.204, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000426 DANKHJ010000000
VERSION     DANKHJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            364..1842
                     /gene="msr(C)"
                     /locus_tag="PW113_003756"
     CDS             364..1842
                     /gene="msr(C)"
                     /locus_tag="PW113_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2527944.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1889..2335
                     /locus_tag="PW113_003757"
     CDS             1889..2335
                     /locus_tag="PW113_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2527945.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2534..3049)
                     /locus_tag="PW113_003758"
     CDS             complement(2534..3049)
                     /locus_tag="PW113_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2527946.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT          989 a          528 c          643 g          961 t
ORIGIN      
        1 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
       61 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
      121 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
      181 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
      241 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
      301 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
      361 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
      421 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
      481 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
      541 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
      601 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
      661 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
      721 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
      781 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
      841 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
      901 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
      961 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
     1021 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
     1081 ccggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
     1141 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
     1201 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
     1261 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
     1321 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
     1381 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
     1441 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
     1501 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
     1561 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
     1621 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
     1681 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
     1741 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
     1801 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt
     1861 cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt
     1921 tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca
     1981 tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt
     2041 cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt
     2101 aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat
     2161 ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt
     2221 ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat
     2281 catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa
     2341 cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc
     2401 tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt
     2461 ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg
     2521 ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt
     2581 ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt
     2641 gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca
     2701 gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag
     2761 catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc
     2821 attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct
     2881 cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga
     2941 tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac
     3001 gtggtttgga taaaagcacg cataataata tataggctaa agtattcata aaaatcctac
     3061 ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat
     3121 t
//
LOCUS       DANKHJ010000427         3121 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.205, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000427 DANKHJ010000000
VERSION     DANKHJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            364..1842
                     /gene="msr(C)"
                     /locus_tag="PW113_003759"
     CDS             364..1842
                     /gene="msr(C)"
                     /locus_tag="PW113_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2527947.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1889..2335
                     /locus_tag="PW113_003760"
     CDS             1889..2335
                     /locus_tag="PW113_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2527948.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2534..3049)
                     /locus_tag="PW113_003761"
     CDS             complement(2534..3049)
                     /locus_tag="PW113_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2527949.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT          989 a          527 c          643 g          962 t
ORIGIN      
        1 gttcgaataa aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt
       61 ggtaacctaa taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa
      121 cttttgaata acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg
      181 agtatgtcca ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat
      241 ttagtcatag ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag
      301 atttttctgc tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa
      361 catatggaaa atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta
      421 gaactatcta ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa
      481 aatggtgtcg gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat
      541 gggaaaatcc aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa
      601 gcaaaggacc taaatttgga attagccagt catttccagt taagactgga agaaacttca
      661 gagcggaaat ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct
      721 tatgaacaag ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat
      781 cgactgattg aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc
      841 tattttctag atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag
      901 ttttcgggga attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta
      961 cgagaagcag agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag
     1021 aaaaaacaag cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga
     1081 ctggatcggt tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat
     1141 gcgaaaacat tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg
     1201 aaacaaatcc gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg
     1261 ggacaaaatg tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag
     1321 ttttctttag gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta
     1381 ttagaacata tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa
     1441 gtatatcagc aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg
     1501 agacaaacag agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct
     1561 caggaaactt tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg
     1621 attgctttgc tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt
     1681 attgatatgg caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt
     1741 ttgtttactt ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata
     1801 aaagggcaga aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt
     1861 cgtgacaaat ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt
     1921 tacaacaatg atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca
     1981 tcaagatctt catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt
     2041 cattttggat gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt
     2101 aaatccagaa agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat
     2161 ggtgacatgt atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt
     2221 ctctgcttat atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat
     2281 catcgttggc ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa
     2341 cactgttatt tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc
     2401 tagaaaaata gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt
     2461 ccaactgtat tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg
     2521 ttctgtttat cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt
     2581 ggagttcctg ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt
     2641 gtttcttgtc tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca
     2701 gctgttgttc tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag
     2761 catagatttt tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc
     2821 attcctctac aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct
     2881 cgtctttgtc caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga
     2941 tctgactgtg atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac
     3001 gtggtttgga taaaagcacg cataataata tataggctaa agtattcata aaaatcctac
     3061 ctctcgtctt attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat
     3121 t
//
LOCUS       DANKHJ010000428         3118 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.206, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000428 DANKHJ010000000
VERSION     DANKHJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            <1..1311
                     /locus_tag="PW113_003762"
     CDS             <1..1311
                     /locus_tag="PW113_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL2527950.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1441..2613)
                     /locus_tag="PW113_003763"
     CDS             complement(1441..2613)
                     /locus_tag="PW113_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL2527951.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1085 a          405 c          559 g         1069 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg agtcaagtcc agactcctgt gtaaaatgct atacaatgtt tttaccattt
     1441 ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat gtaggtccat
     1501 aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc gaataatctt
     1561 ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct ttagtcgatt
     1621 gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat ccaatgatgc
     1681 gcaagctttt gaatattttg gttgatcgat ataatcatga atcaatcgat ttttagcctc
     1741 acgcgctaag ttaatatctg tgaacttaaa aattccttta acagcttctc tgaaagattt
     1801 tgaatttttt ttaggaatgg tggtaaagat atttcttagg aagtgaactt ggcatctttg
     1861 ccaacttacg ttggtgaagg attttctaat ggcagagact aatcctttgt gcgcatcaga
     1921 aataacgagt tccgtacctt gtaaaccgcg ttcttttagg tattcaaaaa atgttgtcca
     1981 ggtctcttcg ctttcgccac tttgaatcat gaagccgata atttcacggt cgccatcttt
     2041 ggttattcca atcgctatat gacagctttt tgagagtact cgattttctt ctcgtacttt
     2101 tatatagagt acatcggtca ttaagtaagg ataatttttt tctgataata aacgattctg
     2161 ccactcgtta accataggtt ctagctgttc tgttaagcta gaaacgaagg acttagagac
     2221 ggatttacca caaagttctt ccacaatttt tgatacttta cgagttgaaa cgcctgatac
     2281 atacatttcc aacattgaag ccatgagggc tttttcgttt cgttgataac gttcaaacac
     2341 tgtgggtgaa aaatggccat cacgtgttct gggtactttt aattctagcg tgcctacacg
     2401 tgtcgtaaag ctgcgctcat aatagccatt tcgttgactt tgtcggtttt ctgttcgttc
     2461 atattctttt gcttgaatat attctgttcg ttgattttcc attagttgat taaataccgt
     2521 tgttaaaata tttttagaaa cgtcatcctt tacagaatat tcaataatgc tttgaatctc
     2581 ttcgcttttc agtgtaaaat gtacttgggt catgtaaaag tcctcctggg tatgtttttg
     2641 tcgttaaaaa cattgtaccg taaaaggact gttatatggc ctttttactt ttacacaatt
     2701 atacggactt tatctatata aaggcgctgt cagagttttt tgacttagaa catacattca
     2761 ttcaagagac tttaaacaag ctagcagaac gcccaaaaac ggacacacaa ctcgatttgt
     2821 ttagctatga tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga
     2881 tacgtgtttg tttttctgta ctgtttcctg agtgattagc agaaatataa agattaaaat
     2941 tttaattgat tattaggaag agaaggagaa tgtatcccga aaacttttta gttggcttgg
     3001 actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg gagtgggagc gaagcgcaca
     3061 cttgatcttt tgaacttttt tcggttctgt tgcaaagttt taaatctact atcaaata
//
LOCUS       DANKHJ010000429         1781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538950-rid17234053.guided.263, whole genome shotgun
            sequence.
ACCESSION   DANKHJ010000429 DANKHJ010000000
VERSION     DANKHJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538950
            Sequence Read Archive: ERR10688513
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638267.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638267.1
            Genome Coverage       :: 257x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:17:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,765
            CDSs (total)                      :: 3,702
            Genes (coding)                    :: 3,608
            CDSs (with protein)               :: 3,608
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 41 of 94
            Pseudo Genes (incomplete)         :: 41 of 94
            Pseudo Genes (internal stop)      :: 25 of 94
            Pseudo Genes (multiple problems)  :: 11 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538950-rid17234053.guided.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2021-08-07"
     gene            24..416
                     /locus_tag="PW113_003764"
     CDS             24..416
                     /locus_tag="PW113_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2527952.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="PW113_003765"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="PW113_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL2527953.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
